LOCUS       DAUCWO010000001       101957 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUCWO010000001 DAUCWO010000000
VERSION     DAUCWO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 101957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101957
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.001"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(19..1518)
                     /locus_tag="VGY20_000001"
     CDS             complement(19..1518)
                     /locus_tag="VGY20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4272895.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILVLLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            complement(1508..2854)
                     /locus_tag="VGY20_000002"
     CDS             complement(1508..2854)
                     /locus_tag="VGY20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4272896.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNYRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGNSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            complement(2963..3664)
                     /locus_tag="VGY20_000003"
     CDS             complement(2963..3664)
                     /locus_tag="VGY20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ4272897.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            complement(3675..5504)
                     /gene="glpO"
                     /locus_tag="VGY20_000004"
     CDS             complement(3675..5504)
                     /gene="glpO"
                     /locus_tag="VGY20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ4272898.1"
                     /translation="MEFSKEIRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQT
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVIKEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQ"
     gene            complement(5520..7046)
                     /gene="glpK"
                     /locus_tag="VGY20_000005"
     CDS             complement(5520..7046)
                     /gene="glpK"
                     /locus_tag="VGY20_000005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ4272899.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     INTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDTK
                     "
     gene            complement(7407..7799)
                     /locus_tag="VGY20_000006"
     CDS             complement(7407..7799)
                     /locus_tag="VGY20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ4272900.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            complement(7926..8183)
                     /locus_tag="VGY20_000007"
     CDS             complement(7926..8183)
                     /locus_tag="VGY20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ4272901.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            complement(8337..10376)
                     /locus_tag="VGY20_000008"
     CDS             complement(8337..10376)
                     /locus_tag="VGY20_000008"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ4272902.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENHLSFEDI
                     QTFSTPRRLAVRVSGLAAQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLITS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVARAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKATPKDIREAVLESSTFVVPEMLAAAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            complement(10753..11670)
                     /gene="glyQ"
                     /locus_tag="VGY20_000009"
     CDS             complement(10753..11670)
                     /gene="glyQ"
                     /locus_tag="VGY20_000009"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ4272903.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEMSDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDGE"
     gene            complement(12041..12574)
                     /locus_tag="VGY20_000010"
     CDS             complement(12041..12574)
                     /locus_tag="VGY20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ4272904.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            complement(12706..13545)
                     /locus_tag="VGY20_000011"
     CDS             complement(12706..13545)
                     /locus_tag="VGY20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ4272905.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLGRLGLAYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVYHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTPEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            complement(13667..14815)
                     /gene="nagA"
                     /locus_tag="VGY20_000012"
     CDS             complement(13667..14815)
                     /gene="nagA"
                     /locus_tag="VGY20_000012"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ4272906.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKNPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLIHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            complement(14933..16564)
                     /locus_tag="VGY20_000013"
     CDS             complement(14933..16564)
                     /locus_tag="VGY20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ4272907.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            16766..17488
                     /locus_tag="VGY20_000014"
     CDS             16766..17488
                     /locus_tag="VGY20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4272908.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILL
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            complement(17617..18459)
                     /locus_tag="VGY20_000015"
     CDS             complement(17617..18459)
                     /locus_tag="VGY20_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4272909.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYAGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            18752..19309
                     /locus_tag="VGY20_000016"
     CDS             18752..19309
                     /locus_tag="VGY20_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4272910.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            complement(19346..20170)
                     /locus_tag="VGY20_000017"
     CDS             complement(19346..20170)
                     /locus_tag="VGY20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4272911.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(20172..20789)
                     /locus_tag="VGY20_000018"
     CDS             complement(20172..20789)
                     /locus_tag="VGY20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ4272912.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            complement(20986..21963)
                     /gene="lacD"
                     /locus_tag="VGY20_000019"
     CDS             complement(20986..21963)
                     /gene="lacD"
                     /locus_tag="VGY20_000019"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ4272913.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            complement(22010..22463)
                     /locus_tag="VGY20_000020"
                     /pseudo
     CDS             complement(22010..22463)
                     /locus_tag="VGY20_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(22473..22988)
                     /gene="lacB"
                     /locus_tag="VGY20_000021"
     CDS             complement(22473..22988)
                     /gene="lacB"
                     /locus_tag="VGY20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ4272914.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLETHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            complement(23003..23428)
                     /gene="lacA"
                     /locus_tag="VGY20_000022"
     CDS             complement(23003..23428)
                     /gene="lacA"
                     /locus_tag="VGY20_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ4272915.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            complement(23685..25136)
                     /locus_tag="VGY20_000023"
     CDS             complement(23685..25136)
                     /locus_tag="VGY20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ4272916.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFSVESVKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIESVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            complement(25162..25467)
                     /locus_tag="VGY20_000024"
     CDS             complement(25162..25467)
                     /locus_tag="VGY20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4272917.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            complement(25460..25933)
                     /locus_tag="VGY20_000025"
     CDS             complement(25460..25933)
                     /locus_tag="VGY20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4272918.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSCLASLKDDKQAISNYLIEKGVFQHD"
     gene            26170..26940
                     /locus_tag="VGY20_000026"
     CDS             26170..26940
                     /locus_tag="VGY20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4272919.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            complement(27144..27347)
                     /locus_tag="VGY20_000027"
     CDS             complement(27144..27347)
                     /locus_tag="VGY20_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ4272920.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(27361..29592)
                     /locus_tag="VGY20_000028"
     CDS             complement(27361..29592)
                     /locus_tag="VGY20_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ4272921.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGRAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTYQAIQKLLSLKATDVRVWRNSQWQLLPLEEISYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGQGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFIQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQNLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNATLMATLGIDLASVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVITNLQSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            complement(29592..30026)
                     /locus_tag="VGY20_000029"
     CDS             complement(29592..30026)
                     /locus_tag="VGY20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ4272922.1"
                     /translation="MQQISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKTL
                     IGRLVKKNLLTSYREGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYDL
                     PMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            30198..31184
                     /locus_tag="VGY20_000030"
     CDS             30198..31184
                     /locus_tag="VGY20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4272923.1"
                     /translation="MKHPIRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKR
                     DKSYYSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKP
                     DQKTIIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVN
                     LYQHYYHKNQNIFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEI
                     PEYEDADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFY
                     PDIRDYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTP"
     gene            complement(31318..31668)
                     /gene="rbfA"
                     /locus_tag="VGY20_000031"
     CDS             complement(31318..31668)
                     /gene="rbfA"
                     /locus_tag="VGY20_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ4272924.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRDLDNKS"
     gene            complement(31874..34735)
                     /gene="infB"
                     /locus_tag="VGY20_000032"
     CDS             complement(31874..34735)
                     /gene="infB"
                     /locus_tag="VGY20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ4272925.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNINAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVVEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            complement(34755..35057)
                     /locus_tag="VGY20_000033"
     CDS             complement(34755..35057)
                     /locus_tag="VGY20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ4272926.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            complement(35050..35346)
                     /locus_tag="VGY20_000034"
     CDS             complement(35050..35346)
                     /locus_tag="VGY20_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ4272927.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            complement(35362..36519)
                     /gene="nusA"
                     /locus_tag="VGY20_000035"
     CDS             complement(35362..36519)
                     /gene="nusA"
                     /locus_tag="VGY20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ4272928.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEELVVDDVMTAEKV"
     gene            complement(36694..37230)
                     /gene="rimP"
                     /locus_tag="VGY20_000036"
     CDS             complement(36694..37230)
                     /gene="rimP"
                     /locus_tag="VGY20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ4272929.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            complement(37338..37424)
                     /locus_tag="VGY20_000037"
     tRNA            complement(37338..37424)
                     /locus_tag="VGY20_000037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37388..37390),aa:Ser,seq:gga)
     gene            complement(37489..38124)
                     /gene="trmB"
                     /locus_tag="VGY20_000038"
     CDS             complement(37489..38124)
                     /gene="trmB"
                     /locus_tag="VGY20_000038"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ4272930.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            complement(38124..38915)
                     /locus_tag="VGY20_000039"
     CDS             complement(38124..38915)
                     /locus_tag="VGY20_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ4272931.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            complement(38979..40013)
                     /locus_tag="VGY20_000040"
     CDS             complement(38979..40013)
                     /locus_tag="VGY20_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4272932.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            complement(40016..40741)
                     /locus_tag="VGY20_000041"
     CDS             complement(40016..40741)
                     /locus_tag="VGY20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4272933.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            40813..41232
                     /locus_tag="VGY20_000042"
     CDS             40813..41232
                     /locus_tag="VGY20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ4272934.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            41229..41585
                     /locus_tag="VGY20_000043"
     CDS             41229..41585
                     /locus_tag="VGY20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ4272935.1"
                     /translation="MKVKTMIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQGDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(41696..42979)
                     /locus_tag="VGY20_000044"
     CDS             complement(41696..42979)
                     /locus_tag="VGY20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ4272936.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(42988..43512)
                     /locus_tag="VGY20_000045"
     CDS             complement(42988..43512)
                     /locus_tag="VGY20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4272937.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSRTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            complement(43487..43948)
                     /gene="tsaE"
                     /locus_tag="VGY20_000046"
     CDS             complement(43487..43948)
                     /gene="tsaE"
                     /locus_tag="VGY20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ4272938.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            complement(44102..45562)
                     /locus_tag="VGY20_000047"
     CDS             complement(44102..45562)
                     /locus_tag="VGY20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ4272939.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFNSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            45826..46638
                     /locus_tag="VGY20_000048"
     CDS             45826..46638
                     /locus_tag="VGY20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4272940.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALGYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTRHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNINKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            46990..47982
                     /gene="manX"
                     /locus_tag="VGY20_000049"
     CDS             46990..47982
                     /gene="manX"
                     /locus_tag="VGY20_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ4272941.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            48071..48880
                     /locus_tag="VGY20_000050"
     CDS             48071..48880
                     /locus_tag="VGY20_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ4272942.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            48897..49808
                     /locus_tag="VGY20_000051"
     CDS             48897..49808
                     /locus_tag="VGY20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4272943.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            49922..50281
                     /locus_tag="VGY20_000052"
     CDS             49922..50281
                     /locus_tag="VGY20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ4272944.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTTINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            50673..51950
                     /gene="serS"
                     /locus_tag="VGY20_000053"
     CDS             50673..51950
                     /gene="serS"
                     /locus_tag="VGY20_000053"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ4272945.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(52183..52953)
                     /locus_tag="VGY20_000054"
     CDS             complement(52183..52953)
                     /locus_tag="VGY20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ4272946.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(52950..53816)
                     /locus_tag="VGY20_000055"
     CDS             complement(52950..53816)
                     /locus_tag="VGY20_000055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ4272947.1"
                     /translation="MALFRKKDKYIRITPNNFLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            complement(53825..55189)
                     /locus_tag="VGY20_000056"
     CDS             complement(53825..55189)
                     /locus_tag="VGY20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ4272948.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     SIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            complement(55221..55643)
                     /gene="fabZ"
                     /locus_tag="VGY20_000057"
     CDS             complement(55221..55643)
                     /gene="fabZ"
                     /locus_tag="VGY20_000057"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ4272949.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            complement(55640..56140)
                     /locus_tag="VGY20_000058"
     CDS             complement(55640..56140)
                     /locus_tag="VGY20_000058"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ4272950.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            complement(56142..57374)
                     /gene="fabF"
                     /locus_tag="VGY20_000059"
     CDS             complement(56142..57374)
                     /gene="fabF"
                     /locus_tag="VGY20_000059"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ4272951.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            complement(57389..58123)
                     /gene="fabG"
                     /locus_tag="VGY20_000060"
     CDS             complement(57389..58123)
                     /gene="fabG"
                     /locus_tag="VGY20_000060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ4272952.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            complement(58113..59051)
                     /gene="fabD"
                     /locus_tag="VGY20_000061"
     CDS             complement(58113..59051)
                     /gene="fabD"
                     /locus_tag="VGY20_000061"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ4272953.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            complement(59076..60047)
                     /gene="fabK"
                     /locus_tag="VGY20_000062"
     CDS             complement(59076..60047)
                     /gene="fabK"
                     /locus_tag="VGY20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ4272954.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAQRWQSVSIEK"
     gene            complement(60248..60472)
                     /locus_tag="VGY20_000063"
     CDS             complement(60248..60472)
                     /locus_tag="VGY20_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ4272955.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            complement(60533..61507)
                     /locus_tag="VGY20_000064"
     CDS             complement(60533..61507)
                     /locus_tag="VGY20_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ4272956.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            complement(61508..61942)
                     /locus_tag="VGY20_000065"
     CDS             complement(61508..61942)
                     /locus_tag="VGY20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4272957.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFSDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            complement(62019..62810)
                     /locus_tag="VGY20_000066"
     CDS             complement(62019..62810)
                     /locus_tag="VGY20_000066"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ4272958.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            complement(63030..64166)
                     /gene="dnaJ"
                     /locus_tag="VGY20_000067"
     CDS             complement(63030..64166)
                     /gene="dnaJ"
                     /locus_tag="VGY20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ4272959.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(64447..66282)
                     /gene="dnaK"
                     /locus_tag="VGY20_000068"
     CDS             complement(64447..66282)
                     /gene="dnaK"
                     /locus_tag="VGY20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ4272960.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(66463..67035)
                     /gene="grpE"
                     /locus_tag="VGY20_000069"
     CDS             complement(66463..67035)
                     /gene="grpE"
                     /locus_tag="VGY20_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ4272961.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(67038..68072)
                     /gene="hrcA"
                     /locus_tag="VGY20_000070"
     CDS             complement(67038..68072)
                     /gene="hrcA"
                     /locus_tag="VGY20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ4272962.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(68207..68776)
                     /locus_tag="VGY20_000071"
     CDS             complement(68207..68776)
                     /locus_tag="VGY20_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4272963.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(68773..69516)
                     /locus_tag="VGY20_000072"
     CDS             complement(68773..69516)
                     /locus_tag="VGY20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ4272964.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQESAEALVQTYLQPPGA
                     SEHHTGLAIDISTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(69506..70213)
                     /locus_tag="VGY20_000073"
     CDS             complement(69506..70213)
                     /locus_tag="VGY20_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ4272965.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEIDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(70605..70844)
                     /locus_tag="VGY20_000074"
     CDS             complement(70605..70844)
                     /locus_tag="VGY20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ4272966.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(71059..71274)
                     /locus_tag="VGY20_000075"
     CDS             complement(71059..71274)
                     /locus_tag="VGY20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4272967.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCLHYY"
     gene            complement(71271..72710)
                     /gene="gatB"
                     /locus_tag="VGY20_000076"
     CDS             complement(71271..72710)
                     /gene="gatB"
                     /locus_tag="VGY20_000076"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ4272968.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSTYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLLD"
     gene            complement(72710..74176)
                     /gene="gatA"
                     /locus_tag="VGY20_000077"
     CDS             complement(72710..74176)
                     /gene="gatA"
                     /locus_tag="VGY20_000077"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ4272969.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFI
                     TVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFN
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(74176..74478)
                     /gene="gatC"
                     /locus_tag="VGY20_000078"
     CDS             complement(74176..74478)
                     /gene="gatC"
                     /locus_tag="VGY20_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ4272970.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(74710..74958)
                     /locus_tag="VGY20_000079"
     CDS             complement(74710..74958)
                     /locus_tag="VGY20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ4272971.1"
                     /translation="MIRSRSRPLIRKGLAVSLARSVKPELKLGICGEHGGDPASIAFC
                     HSQGLTYVSCSPFRVPLTRLAAAQAAIKASGHSLTQDK"
     gene            complement(75473..76027)
                     /locus_tag="VGY20_000080"
     CDS             complement(75473..76027)
                     /locus_tag="VGY20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ4272972.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSHMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(76174..76956)
                     /gene="codY"
                     /locus_tag="VGY20_000081"
     CDS             complement(76174..76956)
                     /gene="codY"
                     /locus_tag="VGY20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ4272973.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(77174..78388)
                     /locus_tag="VGY20_000082"
     CDS             complement(77174..78388)
                     /locus_tag="VGY20_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ4272974.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            78619..79071
                     /locus_tag="VGY20_000083"
     CDS             78619..79071
                     /locus_tag="VGY20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ4272975.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(79193..80581)
                     /locus_tag="VGY20_000084"
     CDS             complement(79193..80581)
                     /locus_tag="VGY20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4272976.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGVAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            80653..81618
                     /locus_tag="VGY20_000085"
     CDS             80653..81618
                     /locus_tag="VGY20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ4272977.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFNLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(81967..82671)
                     /locus_tag="VGY20_000086"
     CDS             complement(81967..82671)
                     /locus_tag="VGY20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4272978.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYFLGNVFLFSKRELK"
     gene            complement(82684..83586)
                     /locus_tag="VGY20_000087"
     CDS             complement(82684..83586)
                     /locus_tag="VGY20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4272979.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            complement(83879..85894)
                     /gene="recG"
                     /locus_tag="VGY20_000088"
     CDS             complement(83879..85894)
                     /gene="recG"
                     /locus_tag="VGY20_000088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ4272980.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKKEVYD"
     gene            complement(86269..87723)
                     /locus_tag="VGY20_000089"
     CDS             complement(86269..87723)
                     /locus_tag="VGY20_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4272981.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(87660..88340)
                     /locus_tag="VGY20_000090"
     CDS             complement(87660..88340)
                     /locus_tag="VGY20_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ4272982.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(88337..88930)
                     /locus_tag="VGY20_000091"
     CDS             complement(88337..88930)
                     /locus_tag="VGY20_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ4272983.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(88927..90597)
                     /locus_tag="VGY20_000092"
     CDS             complement(88927..90597)
                     /locus_tag="VGY20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4272984.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     RTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            complement(90590..92353)
                     /locus_tag="VGY20_000093"
     CDS             complement(90590..92353)
                     /locus_tag="VGY20_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ4272985.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQEIINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLSTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(92350..93186)
                     /locus_tag="VGY20_000094"
     CDS             complement(92350..93186)
                     /locus_tag="VGY20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4272986.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(93183..94205)
                     /locus_tag="VGY20_000095"
     CDS             complement(93183..94205)
                     /locus_tag="VGY20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4272987.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(94207..95091)
                     /gene="isdE"
                     /locus_tag="VGY20_000096"
     CDS             complement(94207..95091)
                     /gene="isdE"
                     /locus_tag="VGY20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ4272988.1"
                     /translation="MIKRCKGIGLALMVFFLVACVNQHSKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(95075..95950)
                     /locus_tag="VGY20_000097"
     CDS             complement(95075..95950)
                     /locus_tag="VGY20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ4272989.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(96147..99974)
                     /gene="shr"
                     /locus_tag="VGY20_000098"
     CDS             complement(96147..99974)
                     /gene="shr"
                     /locus_tag="VGY20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ4272990.1"
                     /translation="MKKISKCAFVAISTLVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVIITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEELSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKSSNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(100464..>101957)
                     /locus_tag="VGY20_000099"
     CDS             complement(100464..>101957)
                     /locus_tag="VGY20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4272991.1"
                     /translation="LSLLAPTAESMTVLAQDVMLETHKATTNETSDSSSKEENNKNAA
                     PTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKAEAENQVDDKSTD
                     HSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPIQSLSPYVSDLDL
                     SEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYDSTRING
                     EKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQASKFND
                     DSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSGKRRAQI
                     MEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSYSWGQCT
                     WYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAGADGTYGHV
                     SIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
BASE COUNT        30973 a        21944 c        17850 g        31190 t
ORIGIN      
        1 tttagaaaag accaagtatc acacagtttt gtgtgcaaaa ctagacttag ctgctgggct
       61 gagtgagagt tcctcttctc gtgtattttt ttgctggaaa aggtctttga gaaaagcttc
      121 taaggcagtg agttcatagg gggagctata ctgttttaac cggtaatagg gtaaatcttg
      181 tctcggtaaa cagacagtct gataggtaat cagacaatca taaactttct gatgatcata
      241 gggcattaat tgaaaatccc tacgatgccc caaatgacgt ctaatggcca attccgcaat
      301 ggctgcttcc accttatttt gccccaaggc aagccctact aaaagaggct catggtggcg
      361 ctcaatggaa aaaatcaaca atactaaaat gtcgtcactc aactgctgac tgaaatccgt
      421 ttcctgaccc atttgcagca ctaaatggtt tatgataacc cttaaaagcg ctcttttatc
      481 ttcgcctact aattgataaa ggtattggct attggttggt tgactaagga tagctccttt
      541 aaaaaagtaa gctttggaaa aaaatatccc caagccataa atgatttcct cctttgtctg
      601 atggataatc acatgagcct tctttaaaag ccataaggct tctgaaatgt gatcagcaat
      661 aggacctcca aaacccaaac tatacttcat gctggcaata ggaagtagag gataagcatg
      721 caaaaagaca aagaggctct tagcttcaaa agcatcaaat tcaagggcat agcgtcttaa
      781 gtaatgtaaa agcatgcttt ctaagcgctt aaagaaaata ttagatgtca taaagtcact
      841 atctcttaaa aaatgatcat tgtagccttt ttgaaaggac attcgcgctt gcgaaatcgc
      901 caaccaaatc aataattgtt ctaaggcttc tgccgataat gactggccaa tcagccgctc
      961 aattaaggta gccaaatgcg aagcgtctaa ctgattgacc aaggcgtcta tgccttgtct
     1021 ggtcaaggta tggcggaaca attcaaaata gaaataacgc cactgaagct cacttcccag
     1081 ttgtctgcct tgcgagagcg ccagatcaaa ttctgataaa agctggttga ggtgagctag
     1141 ttgtctgttt agagtagctt cactaaccat cagttcaacg gataagttgg tgatgttaaa
     1201 gtgttcatgg cagtacatgt aggtcaatat ctgataggca accgattctt tgagaagcag
     1261 agctaccaaa tcctcccaat ggaactggcc attttctttt aagctcagac tgtctttttc
     1321 taaaagtaat tgacaggcta aaccttgttc tcgacacaaa tgatttaggt tgtcaatgta
     1381 ttttaatagg gtcactttag acaagtttga tagcctcatt aggtctttta gccttaacgt
     1441 ttctttagca tgataaaggg tcaccaagag acgatattga gcttttcgtt ctttatccat
     1501 taagtcttca atcttcatgg cggagcctct tttcaaataa tttgagacaa gcttcatgaa
     1561 gatgataagc caaatcactg tgaccttgta aaaagagaaa atcttgaaat gctaagtcac
     1621 cgtctgtaac tttcagagcc agagcctgac ctaattggtt ggcgatgaca agagcattgg
     1681 ttttggatag gagctgagcg cctattaaaa tcccatccgt ttttctagct attaatttca
     1741 gccaaaagag gtttttagaa ttcccatttt ggtacaaata gctacacgta tccatataaa
     1801 gctctgcttc ttcttctgtc acaccaacac tcgcgcgata ataaccaaag tgagaactac
     1861 ctaatacctt gaccgtctgt aatggaggaa taggatatcc agctaaatgc agagccagag
     1921 cctgacctgt cttcattgct tggttaatca aaggtgtata gtaatccatt cccaatatac
     1981 caaaatgcaa tgaaaccatg tctccaatgg catagatatt agcctggctg gtctgtaagt
     2041 tttcattgac caaaatggtc ttatccagag cacaagccac ttgtccttgc aaaaggtgtg
     2101 tatttggcct aaagttaatg gctaaaatag ccaaatcacc ttggaaggtg tccccttgct
     2161 cggtatgagc gacaactttt ccctctatct catgaatagc cgtaacacgc ttattattaa
     2221 taaaggtgac atggtgagtg gacatttctg ccattacagg ggctatcagt tctgcgtcag
     2281 tctgatagaa atctggacgt tcggctgctt caatcagttt gacccgtttg ccttgtaagg
     2341 acaaactgta ggcaaggtct aagccaatca agccggcacc aataaccaag acctcttggc
     2401 tagcctcaat cagttttaaa ctagcttggc tttcttcata atatttggtt accaaaactt
     2461 tgttggtctg tgacgtctca atataatgag actgggggct agcacccatg gcacaaacaa
     2521 gcgtttcata accttcttca aaaactcgtc cttgactgtc tttgagaagc aaggtgtttg
     2581 agggagcttc gatagctaac acttctgctt gaataaaccg gtagttagat tctatttgtg
     2641 ctgctaggca ggcccttccc cacatagcat cagacaggtc ttgaatgtct cctctgaata
     2701 attggttgat accatttgga atataattag gacagctttc cttatcaatg agaatgattt
     2761 gggaatctgg attaaggtcc ttatatttat caacaaaggc tagaccagca aaggatgccc
     2821 cgataacatg tatggtcttt gtcgttaatg tcataatagt attattatag cagttgtttt
     2881 taagatatga aagcgtttta cctaatccta taaagaaaaa gcaaaggagc tggcctatga
     2941 tagccaaccc ctttagtgat ggttaggaca tgacttggaa aagcaaaact gctaaagccg
     3001 ctccaataat tggtcctaca acaggtatcc aagcataaga ccaatcagaa tctcccttgt
     3061 tgttgagtgg tagaatagca tgaactagac gaggtcctaa atcacgcgct gggttaatgg
     3121 cgtaacctgt cgttcctcct aaggataatc caatcccaat aaccaaggta cctacacaga
     3181 gggttcctag tcctgctggc atatcatata agccaaatgc caaaatacca agcatcaaga
     3241 caaaggtccc aaagatttca ctcaataggt ttgagctagt atctcgaata gcaggacctg
     3301 tcgcaaatgt ccctaaaata tcagcctgac tctcagcagc cagataatgt ggcctgaact
     3361 gaaggaatac cagagttgaa ccaaccatag ctcccaatag ttgggcaagg ctataggcaa
     3421 tggctgttga ccaagcaatg gttccactca tggcaaaagc aaggctaaca gctggattga
     3481 ggtgggcagg ggcaactttg ccactgataa agactgccac agcaactgca attccccagc
     3541 cagtcgcaat cacgatccag ccagaagcat gggttttcgt tttgggcaag acaacccctg
     3601 ctaccacacc atttcctagt aaaaccaata aggctgtacc caaaaattca ccaaagatat
     3661 ccatattaac cctcctactg gccttttagg gcactcaaat cattgtctgc aatcacgcga
     3721 cgaagatcgt cttcttgtgc cactctttcc tgatcagacc attcaaaatg tttagccatt
     3781 tctttaatca ctgggtcaat caaagcatct agactatctc gcataaataa gaggtgattt
     3841 gttcttctca agaaataatc tgtcggttta agagccattt cataatccat cgcataatgt
     3901 aaggacaagg tttcagctaa acttaaccct tcagctgctg ttaattgacg agttaaggca
     3961 aagacttttg gcgcattaga accataaagg tttgccaaat acctagcatc atccatcgaa
     4021 agaccactaa gagttcctaa ttgagcatag gcttctattt ctgaatctac attggctgga
     4081 ttgatttcac ctcctgaaac aggataggtt tttgaattga taagcttgaa ggatttgcca
     4141 aactcttctt tgagggtttg aataatccct gtcaatgctc cttcagccat tttgcgatag
     4201 tcggtaatct taccacctgc caaggtaaag aggccattct catcacggtc aaaacttgaa
     4261 cctcgtgaca ctgcagacgg atccaattct ttttcagatg tgctggtttc aacctgctta
     4321 atagcttttt caacagcttc tcggctatct tcgtggttaa tataggcttt gacagtatca
     4381 accaagtgat caaaactatc atcactgact ttaccgctgt tgccaccatt gtagtcagaa
     4441 gcactattac ctgataacaa ggggcgaagg ccagcccaac tgctttcaat atcatcaatg
     4501 gtcacgttgg catttggaaa gcggttattg acaacgccta acaaataatc cacatcttct
     4561 tgagtaactt gtgggtgctc caagtctcca gtgtagtccg tgtccgttgt tccaaaataa
     4621 gttttttctt cacgtggcaa gacaaagacc atacgaccat catttaaccc tgtgtcaaca
     4681 tagacaggtt gagacactgg taatttttga cggtccacta ccagatgcac cccttttgta
     4741 gggcgcattt gatgaatcgg ttgtccctta tgagagaatt gacgaatctc atcactccat
     4801 ggacctgtgg tgttgatgac taatttagcc ttgataataa tttcttgatc tgacagcaaa
     4861 tcacgcgcct tcacaccaat aatcttacca ttgtcatcta gcaagaaatc ttctgctttc
     4921 acatgactag caataagagc cccatcacga ttagctcgtt tgatattttc aataacaagc
     4981 cttgcgtcat tattgcggaa atcaaggtaa accccaccac caagcaagcc ttcttgtttt
     5041 aagtctggtt ctcgttttaa gacttcttct ttagttaaca ccttgttggc cgctggcgta
     5101 ttggacacgc ctgctaaaag atcatacaaa tccatagcga ccttcaaacg gaacatgcta
     5161 aaggtactgc caggttcgtc ataaacaggt aataacatag ggtctggttt tggaatgtgg
     5221 ggtgcaattt gttgcaccac agcccgctct gacaccgtat ctgaaaccac ctccacatca
     5281 aattgtttca agtaacgaag ccccccgtga accaatttgg ttgaacggct agaggttcct
     5341 tgcgcaaaat cttgcatctc aatcagaccc gtatctaggc cactagctgc cgcctgaagt
     5401 gccacaccag cacccgtaat acctccccca ataattagta ggtctaaatc tctttcttgc
     5461 attttttgaa gagctaagcg tcttatctct tttgaaaatt ccattccatc ctcctctagt
     5521 tattttgtat cgtcatctgc atcttcttct tgggtgaaaa cttgagttgc tttaacagcg
     5581 cgcttccaac ccttgtataa tttttctttg cgcgattcat tcatgctagc cttaaagagc
     5641 tggcctgtgg catttaactc tttcaaagca tccatgtctt cccagtagcc cacagcaagt
     5701 cctgctaaga aagctgctcc tagagctgtt gtttctaaat ttttagcacg ggcaatatca
     5761 attcctaaaa tatcagcctg aaattgcatg agcatattat tcatggctgc accgccatcc
     5821 acacggagct gttgaatgtc aatacctgaa tccacctgca tggtattaat aacatctcgc
     5881 acttgatagg cgatagattg taaggttgct ttgacaaagt cttctttgct ggtaccacgt
     5941 gtcaagccaa agacagatcc acgcgcatta gaatcccagt aaggagctcc taaaccggta
     6001 aaagcaggca caacataaac ttcatcgtca ctagttgatg ctagagcgaa ctgttcagat
     6061 tctggtgaag tctcaatcat tttaagacca tctcgcaacc attgaatggc agagcctgca
     6121 ataaagatgg aaccttccaa ggcatagtga accttcccat taatcccgta accaatggtg
     6181 gtcaaaagat tattactcga taactgcatc tcatcaccag tattcatgat aatgaaagaa
     6241 cctgtgccat aagtgttttt caccatacca ggctcaaaag ctagttggcc aaacaaagct
     6301 gcctgttggt cacccgccat acctgaaata ggcacttcgc ccccataaaa atgaaaggca
     6361 gctgttttgc cataaatttc agaattggat ttaacctctg gaagcatatc ttttggaata
     6421 tttaaaagct ctaaaatctc atcatcccat gttaagtctt taatattata gagcatggta
     6481 cgagccgcat tggaataatc cgtcacatga acagcaccat ctgtcaattt ccaaaccagc
     6541 caagtatcaa tggttccaaa aagtaactcg cctttttcag ctcgttcttg agcaccagga
     6601 acatggtcca aaatccaacg gatcttggtt gcagaaaagt aagcatcaat aacaaggcct
     6661 gtcttttcat ggatcatttt tgtatggcca tcttgtttga gttgctcagc aattggtgcc
     6721 gtctgtcgtg attgccacac aatagcatta taaataggta cacccgtttt tttgtcccaa
     6781 accactgtgg tctcacgttg gttggtaatt ccgattgctt caatttggct tggtttgatg
     6841 ctggactcaa taaaagcacc cgcaataaca gactgaaccg aattccaaat ctgattagca
     6901 ttgtgctcta cccaaccagc gtgtgggaaa atctgaggga attctttttg gctactgcta
     6961 accttttccc ctttttgatt gaaaataatc gctcgtgaac ttgttgttcc ctgatcaata
     7021 gccatgatat acttttcttg cgacattcat taccctcctt taagtaagcg ttttcctatt
     7081 tgattctatt atagctaatt ttatcagaaa tatattatca agaattttta aagctaaaaa
     7141 aaaagctgat aagataggta gcttatccca ctagatgatc ttgaaaaatc cactgatgac
     7201 aaccacgtct cctcatttct aacgaggtaa tctttcttat caaagaaaat caaaagaccc
     7261 aacaatcact ctgctaatcc agagtcatca ttgggtcata aagagaacta agcctcaaag
     7321 gcaacacggt atcaaacagc cgtgcctaag aggcaaagac aattggacac aagagttaag
     7381 gctgtcttat tcattacagt tcttgattag tcattgattg gttcagaaga tttttccaca
     7441 atatcccctg agttggcgtc aatggtatag ctgtattctt gatctttgtt ttgaaactcg
     7501 atttcataca cggctttgcc atcttcatta tcttgtgtaa ctgatagcat ttgagcatca
     7561 gcttccgtaa cagaagcatc ttttaacgca attgatttgg ctttgtcttc tgaaagttta
     7621 actttcttgc tagtttgact tgtctttggt tgattatcca tatgatcgtc tttatcatcc
     7681 atatcacagg ctgcaagact gagtaaagag aggctggcta tggctagtag agagaatttt
     7741 ttaattgtca taagtactac cttctttctt tgatttcttc tattataact caaacacatg
     7801 gtttttccca atattttgac tatacaatat aaaaacgtgt ggtcacttcg aagtatcata
     7861 ccatttaaaa taaaagctaa cagtcatcac gataaacaaa aagcagttca aagctgcttt
     7921 ttgtcttatg attttgggtc atctaatgaa cggccgtgca agcctttttc acgttgcacc
     7981 tgtcttaatt tttcaggggt cacgtcgttt ccttcttcgt ccaccaattt gatgccttcg
     8041 atatggtgtc tgacagagcg acgatagcct tcaatgtatt cttcacggag ttttgcctgt
     8101 tccacttttt caggtccagt caagcctacc gtttttttct ttttagccag ctcattgata
     8161 cgagcaattt ttttaggatc catgttaagc cactaagagc ctgggaaaaa agtgtaagac
     8221 ttaaaaaact gacttgactc tcactgtgaa aagtaatgtt taaaggcctt gttctttcca
     8281 ctcgggtact gaactcactt ttctagctaa gtccccaacg gctctttcct ttctatttac
     8341 ttggtgttta gttggttaaa agcagcaatg gtcttagctt tgcttactaa atctgataaa
     8401 atagctacac ggttgttctt aagggcttga tcttctgcca taaccattgt attatcaaag
     8461 aaatcattaa tcactgggct aagggcaaag acctgttcta actgctgggc agcactgcct
     8521 gctagagtca agccttgaat agctgcaaac aaagtgtttt cttgctcatt ttcaaagagg
     8581 ctagggtcca cctgaacaga agcgtccgct ttttctgcca aattaaaagc acgtgacaaa
     8641 gattcaactg ctggtttgta attttccgta tggctggctt tgacaagtgc ttcagctgcc
     8701 gctaacattt caggaaccac aaaggttgaa ctctctaaaa ctgcttcacg aatatctttt
     8761 ggtgtggctt tgcccatcat cttatccaca cgagcacgga taaagttcat cacatccgcc
     8821 ttattggcat aggtcaaact atcaaaggac aagtcatata agctatctac cagcttatcc
     8881 attgggatgc gccatccaaa atgatcaaga atacgaacga taccctgcgt tgcccgacga
     8941 agcgcataag gatcattaga gccagatgga atcaagccaa cagaaaagaa tgacaagaga
     9001 gtatccaatt tatctgccaa ggctaggaca gcgccaactt tggtttcagg tagcgcacct
     9061 cctgcagcat caggtaagta gtgttctcgg atagcccttg ccacagcagc atcttcacca
     9121 gctagaagag catatttttc acccataatc ccttgcagct catcaaattc accaaccata
     9181 cctgttaaaa ggtcaaattt gtaaatttgg gcagcacggt caacagctgt gacttcctca
     9241 gcagataggt tggcttcctt agcaagtgat gcggcaatga cacgcgtacg atccatgtgt
     9301 tcagcaagag agccgatttt ttcatggaag gtcacattgg tcaatttggc aaccaaatca
     9361 gcaatttgca atttctgatc ctcacgccag aagaattcac catcttctaa gcgagctacc
     9421 aaaacttttt cgttcccttt aataacattt tcaatggctt gatcattccc attacgaact
     9481 gatacaaagt ttggcatcaa gtgaccagcc tgatcacgta ctacaaaata acgttggtgg
     9541 tttttcattg atgttatcaa tacttcttct gggacatcaa ggtatttagc ctcaaaactt
     9601 cccatgaaag ctgttggaaa ctcgaccaag ttcaaaactt cattaagcaa gtcttcgtca
     9661 atgtcaactt gcacaccctg ctccacttcc agcgtcttga tttgctccac aatcatctct
     9721 tgacgctctt tagcatcagc gataacaaac tggctgcgca aatcagcctc ataagaatca
     9781 gcacttgtga tagtggtttc agtacctaaa aaacggtgac cacggctgac gcgtccagaa
     9841 tgaatatcca agaagtcaag ttccaaggcc tcatcattca acagaactgt caaggtgtga
     9901 acaggacgaa tgtattcaaa actattgtta gcccagtgca tgctgactgg gaaggtcatt
     9961 gctgacaaca cttcagtaac gcctagaagc acttccttag caggttttcc agcttcgtgt
    10021 ttggtcacat aaacgtattc ttctcctttg acttcgcgga attcgatagc atctgtggtc
    10081 aagcctttgc cacgaacaaa tccttgagca gcttttgaga aattcccatc agcatcaagg
    10141 gcaattttct tagcaggccc tttaaagtcc tcagtcaaat ccgtttgctg agcagctaac
    10201 ccagacacac gcactgccaa acggcgtgga gttgaaaagg tttgtatatc ttcaaaagag
    10261 agatgatttt ctgttaagaa agtcgccaag cgctctccca gttgtttttc acttggtgtg
    10321 accacgtagg ctggaagttc ttccagacca agttcgatta gtagattttt actcataatt
    10381 aagataccaa gggcgttaag cggacaccat caaaatagca aagcgacgca ggtgcttgca
    10441 cctaggaagt cttggtcttt ttgacatagt ccgtagcccg tgctcagttc ctttctctaa
    10501 aaagtatctt tttgattgtt cccttaggta gggggacgca aggtgaaatt atccaatgga
    10561 taatttcaga aaacaaaagt gttccattcc tagttagcca gcctaaagtc tcgccaactc
    10621 cctcgttcga taagtaacct tgccgaattg tcactacagc gggagtaacc ttgactcggc
    10681 gaccttcggt cgcccacttc ttgctcgctc ttaagattac aaagctcgca actagatcat
    10741 ctttaaatcg acttattcgc catcttcagc tagcaagatt gcgcgtgttg cttcatccaa
    10801 cagtggaaat ccaagtttct tgcgttctgc aacaaaggtc ttagcaacga cacgtgccaa
    10861 attacggata cgagcaatgt agcctgcgcg ttctgtgacg gaaacagcgc cacgggcatc
    10921 cagcagatta aacgtatgag aacatttgag cacgtaatca taagctgggt gaaccaaacc
    10981 ttcttctagg gcacgcgagg cttctttttc aaacttttca aagttttcaa gcagcatgtc
    11041 ttggtctgac atttcaaagg aatattttga atgctcatac tcaggttgca agaagatttc
    11101 accatattta acacctggtg cccactcaat atcatagacc gaatccactt cttgaatata
    11161 ggaagctaag cgctcaagac cataagttac ttctgctgtc actggagatg tcgcaaggcc
    11221 accaacttgt tggaaatacg taaattgtgt gatttccatt ccatcaagcc aaacttccca
    11281 accaagccct gctgatcctg ttgaggggtt ttcccagtta tcttcaacaa aacgaatatc
    11341 atgttctaaa ggattgatgc ccaatttttc caaagaagca aggtaaagtt cttgaatatt
    11401 tgatggtgat ggtttcatga ccacttgaaa ttggtggtgt tggtaaagac gatttgggtt
    11461 ttctccgtaa cggccatctg caggacgacg tgatggctct acataagccg cattccatgg
    11521 ctcaggacct atagcacgta agaacgtgta aggactcatg gtcccagctc ctttttcatt
    11581 gtcataggcc tgcatgagca tacaaccctg atcattccaa tattgttgta aggtcaagat
    11641 gatttcttga aaggtaagtt ttttagacat ttcgtactcc tttgatagta gtaaaatggt
    11701 atatcattga caatcgtttt ttgtcatcag taaaatacct gacgatgtta ctagcatgta
    11761 ttacaggtct aggcaccaaa aaaccgcatt ttgatgctcc taaatcataa gacctagggg
    11821 cgtcaaaacg cggttccacc ctaatttatt acttcgttta tatagtatct ttttgggcaa
    11881 tattgacaaa tatacttaat cacactgccc aaacggaagc gccaatctgc agtcctacca
    11941 ttcggctcta actaccccga actcgcttta gataagatac ttgcatcctt tttcctagag
    12001 tctagtttag cataaagcca ttagtttgtc catcatgagc ttaaggtttt gttcgagtac
    12061 cgtttggaat aatcacttcc gctaatagct tgttttcttc tcgaatacgt aaatatatta
    12121 ctacaatgta gcatgggaaa agaagcatag ccgtaatttt agcatggcaa agtaaggcta
    12181 tgcccaccag ctcaggggcg atatttaaaa agtagttagg atgtttaatc gttttgaaca
    12241 accagtgatc cacatatttg tggtcttttg ctaacatcaa ttttacagtc caaatgtcac
    12301 ctaagatacg tgtcacttca tataaaacag ctactgataa taacatcaac aaaagaccta
    12361 aaaaactgat gccatcaaac ttaatttgtt taagcagcgc ttcaatcacc gcaaacacat
    12421 aaatcataat gtgaagtaac gtcaaaaatt tcgtgttctg agcaccaaat tcctgtcctc
    12481 cttgggctaa tatggctttt tcatttgtta tcgatttctt caaaaatact aaacgaatag
    12541 taaacatggc taacatcact aaaaagatgg tcacaatacg gctccttatt ttcaataata
    12601 gtttctttca caaaacacta ataaagatta tagctaaatg ataaaccttt gtcaaaccgt
    12661 tttcaaaaaa gatttagcga attatggcta aatctttgta gtttcttaaa aacttgtagt
    12721 atcagggtta ggaatcgctg acattcctga gagatagcga attgtttttt tatcttctgg
    12781 tgtcaagctc acatcaaaaa tagccatatt ttccttgatg cgctcatcat gcaccgattt
    12841 tggcaaggga ataaaacctt ctgccagcga ccaagctaag gcaacttgtg ccactgtttt
    12901 gtcatattta ttagctaact gctgcatagt ctcattatca aaaatctccc cttgtcctag
    12961 aggactccaa gcttccaata aaatctcatt tgccttacaa taatcaacca cttccttttg
    13021 atagcaacct ggggctaatc gaatctgatt aattgctgga gttattttag ctgtctcttg
    13081 aagagcttct agatgataca ccataaaatt gctgacgcca atgcttttaa taagccctgc
    13141 ttcaaccgcc tcttccatat attgccatgc ttgggcattt gcctctttcc aagtgttacg
    13201 cagagctttt gggtttggcc aatggataag atagagatcc acataagcca gaccaagacg
    13261 gccaagagaa gcagctaaag cgtcttttgc cccttcataa ctatgggcgt cattccagag
    13321 tttagttgta ataaataagt cttctcgtag aaccccagaa tccttgatgg cacgacccac
    13381 actttcttca tttttataaa tagctgcagt gtcaatatga cgatagccag ccttgatggc
    13441 tgctaaggta gattggtaag cttcctcacc atcggctgct tggtaagttc caaatcctaa
    13501 aacgggaatc tcatagccac ttgtcatttt tactgttgtt accatcacaa aaacctcctt
    13561 ttatcaatct ctgtaagcga ttactgtaat ctattctatc ataaagaaag aaaatacaaa
    13621 aagaattggc ctgccagaca tagctacccc aatccttttg tatctcttag gcatcaaaag
    13681 ctttttgccc atttaaatag gttgcaacaa gtgttaaatc cttatcaaga acaataaagt
    13741 cagccgcatg cccagctttg atttgaccac atacgtcgtc aataccgaca gaaacagctg
    13801 gcacataggt tgccatatga atggcttctg ccggagttgc gattccccag gccactacat
    13861 ttctaaggcc gtcttttaat ttcaaaatag atcctgccaa gtttccactc tctttaagac
    13921 gagcagtgcc atttgccaca accacagaga attcccccaa gaggtaatca ccatctggtg
    13981 aaccacctgc tcgcatacag tccgtaatca tggctacatg atcatgccct ttttgctgca
    14041 tcaaaatatc acaagcaatt ggtgagacat ggtggccatc acaaatcaac tctgcgtaag
    14101 tatttggaag attataaacc gctcccacca ttcctggttc acggtggatc aatcctcgca
    14161 tgccattata agcatgaacc caaacactag caccagcttg aacagcttct ttagcctctt
    14221 gataagtacc gtttgaatgc cctaaggcca ctgttacgcc ttgttttgtc actgcggaca
    14281 caaattcttt gacaccatca cgctcaggag caagggcaat ttttttgatg agccctttag
    14341 cggctttttg ccaagcatca aattcctcca agcgtgggtt tttcatataa cttgggtttt
    14401 gagcaccctt atattcttcg gtaaaataag gaccttcaaa gtaaatgccc tgaatcttag
    14461 cccctttaac ttgatcagcg actgaagcaa tagtccctga tactttttct aactgctcaa
    14521 aggtagaagt taaggtcgtc ggtaaaaaag acgttactcc tgttgctaat agtccttcac
    14581 tcatttgatg aatcccttgg gcagaattat ccatgacatc agctcccgca tagccatgga
    14641 tatgcgtatc cacaagacct ggtgcaattt gataaccggt gtagtcaatg atttgcgcat
    14701 ctgccggaac aatttctgtc cactccccaa aaacaccatc atgaagactt aggtagccag
    14761 ccggtttcac ctctgttggg taataaaaac aatctgcttt aagatagcat gtcatctttt
    14821 ttcctccctt ttttccttat tatacgcctt attctttact ttgtaaatgg tatataccac
    14881 tttttggatt caaaaaagaa aacgtggatg atcattcacg ttttctcttt ttttaaagct
    14941 ggtgtgaaag tactttctct gctaaattca aggcatgatc tgtgatgcgg gtatagtggg
    15001 aaataatatc aataaaatta atacctgctt gtgccgtaca ttctccacaa ttcaagcgct
    15061 taatgtgggt tttacgtaat cgtcgctctt tttcttcaat ttctttatgg cgagtcacaa
    15121 tagtttgcgc caaatctgta tcactatcaa cgatagctct aatggcatcc agtgttaaac
    15181 aatgcgtcaa ttgatacatc tctgtcaatt cttgacgagc agatattgaa aaaccaattt
    15241 gtttagatat gatgccttca attagaatgc ctaaagactc agaatgatct ccaattcttt
    15301 ccaaatcacg tgaagagtca agaataccag ccaagacttc attttcactt gggctgaggg
    15361 cttcgttaga aatatcaacc aaataagtgg ttaactcctc atcgatggta tcaactgctc
    15421 tttcataacg cttgaccttt tccccaaatt tatcgtcagc tgtcataata taactgtaag
    15481 aagcttcaaa agcttgaatc gcataagagg ctaaatgaac caattccttg tgggcatttc
    15541 ctagtgcgat agaaggggct tgggtaataa gtaagcgatc taaataaagg gcttcgtatt
    15601 tgaccacttc atcttctcct ggaatgagtc tagtcacaat catagctagt agactaatga
    15661 aaggaattaa taagatggta ttagtgatat taaaagagcc gtgagaaaaa gcgatagtca
    15721 tctcaggtgt taaactcaac tttgattgta gccataacat caatgatgtg aatgggacta
    15781 ggataatcat aaaaataatt gtaccgatca aattaaataa cacatgagct gctgccacac
    15841 gtttagcagc aatatttgaa cctatagctg ctaagacagc tgtaatgcag gttcctatat
    15901 ttgagcctaa caaaatagga atagccccct gtaaggtcaa aagaccgcct gaaaacagac
    15961 cctgtaaaat cccgataatg gctgcagatg actgaatcaa catggtcaaa gccgttccga
    16021 taaagacacc ttggaagggt ttatcaccta atgtcgctaa ataattttga aaggctgaca
    16081 cagatttcaa aggatccatg gcatctccca ttaggttcag cgagaagaaa attcctccga
    16141 caccaaaaat gatacgccca aaattgttga gctttttatt agaggtaaag aaaagacagg
    16201 ctgcaccaat aaaaatcatg ggcaaagcgt aatctcctag tttaaaacca attaaaaacg
    16261 aagtgatagt cgttccaata ttagccccca taacaattcc aatggcttgt cgcaaattta
    16321 gcaaacccgc agaaaccaaa ccaaccgtaa tcacagtgac gcctgaactg gactgaatta
    16381 aggcagacat ggcaattccg actaagatac caaaaaaggg attactggtg tatttatcaa
    16441 tgtaatacct tagtttatct cctgctgctt gttgcagacc atctcccata tacttgatac
    16501 tgaaaagaaa gagacctaag cctccaaaga aatgaaatgc aatgtcttgc caatttactg
    16561 acacaatatt gtcctcgctc aattttttag tctattctat tttaatgtat tacggtcttt
    16621 atcacaaggt ttttttaagc gtttctgtgt ataccgatgt caacatttgc atattattgg
    16681 tttgataagg tgttaggctt ttccgatatt ttacatcatt tgtttttgca caaatctttt
    16741 catttcgttt aattagaggt ataatatggc tacgatgaaa caatatcata aaggagattc
    16801 catgactaaa tcacaaaaag aggcgcttta ttggatgttg agtgttctaa ctatcactct
    16861 aataggtggc tcctgcctta tttttggatc ccaccctcaa acgcaagata aggtagctaa
    16921 acattctaaa agtgctgcta gtttactaaa aaaagcagtt aaagcagtta atgacgctga
    16981 tcgtttagcc actgcagctg ctatccaaga agcccagaaa gctgttgata aattagcaga
    17041 atcttctaag aaaaaaacgc tacaagaaca gctaaacgtt gccaaagcaa agcaagagca
    17101 agaagatgca gctactcaag cagttaaagc tgctgaggag actctcaatc aaaatcttaa
    17161 agatatcgcc caaaaagcag ttaacgactt aagtaacaaa ggtaaaaaag ccgctttaca
    17221 atctcggtta gacgctattt tactagcgaa acccattatt gatgaatttc caagacaaag
    17281 tggagaaata acagataact cttactggac acctttccca ggagacgtct cagatactta
    17341 tgataattcc cagtcaccta ctttagaccc aagctcagaa tcttcagctt cagatgttac
    17401 gccacaacct agtcatcctg atccaattcc acctcagacc tcttcagaac cttccgactc
    17461 aggagacaag caatcatcaa aagagtaata caaaagtttt tcgtaaaatc aatcaataaa
    17521 cgttttagac gactatctta gtgattagtc ttcctgacat cttaaaaaag ctagctcaaa
    17581 tcaaaagagt ctagcttttt gttttgttag atagcattaa atttcgtagc ccattaaaag
    17641 tcctccgtct tcagcataaa agctacacaa acctgaggtc cttccagttt gaatcacagc
    17701 atcaggatat aaagatttga ttttttcact gatctgctca caaatttttg gattttgagc
    17761 atgagcaatg taaacttttc cgcctgcata accctctttt tgaatttctt ctatcaaagc
    17821 agaaactgct tttttttgac cacgcgcttt ttgaagaagt tcaagcgttc ctttattgct
    17881 agctttccca accattctaa tattgagtaa gccaattact ttacctacta atttactcag
    17941 tcgaccattt ttaaccaaat tatcaacctt ggcaagcaca aaaatcaaac gtgttttttg
    18001 ctgataagct gtgatctgtt tgacaacttc ttcaaaggat aatccaagat tgatcaaccg
    18061 ctcaagctcc agtacgagca aatccatctc gccacctgct gataaagaat cgattaggtg
    18121 aatattgaca ttaggatttt cttcaagaag ttcattttta gccagtcgtg cactattatg
    18181 actgccagat aaggtccctg taattgtcat aacgataaca ttatctgcac ctctataggc
    18241 ctgcaaaaag gcttctgggc ttggacagct agatgtcgtt gctttagacg actggtacat
    18301 agttgtcatc atattatcga tatctaatcc atcatcatct ctaaaaattt ctgtgccaat
    18361 ctgtagggtc aaaggcacac gttcaaagcg caactctttg gattgtctag tcaaacttct
    18421 aagatcacat cctgaatctg ttacaatttt ccaagtcatt ttttatcctt ctttttctct
    18481 aatttgtgtt cggtaacaaa gaccagatta ttaagccttc gctaagatgt ctaggctaat
    18541 ggtaataact aatcattaaa tcctatgttt tattactagc cttttcaata gttagtaaag
    18601 tcatccaata gagctataca aggaggtaat gaggctcctg ataactaact ttagttgaca
    18661 aactaagact tttcccataa aattatatca ttattaagtc ggtttttgct actgataact
    18721 catcagatgt aacacttgaa aggtatttgt gatgactgat aaagcactct caaaaagatc
    18781 acttcaaaac ttaacacaat ttaataaaga aacacgacgc atagcacgtg aatctatgga
    18841 aatcgcctta ctaaatcttc tagaaacaaa acctttaggt gatattacga tttcagaatt
    18901 ggtgacgaaa gcaggagtat ctcgtaacgc tttttatcgt aattatacct ctaaagaagc
    18961 cattatagaa caactgttag taggggttat ccgtcgtatt tttcgtggac taaaacaatt
    19021 tgatattaaa actcaggctt atcaagcctg gctctatctt tttactgaag ctaaaaaaga
    19081 agctcagctt cttaaaatga ttttcaaaca tcacctgcac catcttctaa cacgattagt
    19141 cactaaacgt ctgaaagctt atcaaaaatt aaaagacaaa aaacaatcgc actataagcg
    19201 ccttttttgg agcaatgcta ttgtttctgt cttgacaaac tggatttcag atgacatgat
    19261 cgttcctgcc gaagaaatgg ctgctatggg cttgcctcta ttaacgtaaa aaggttcctt
    19321 ttgggaacct ttttttatat aatagttagt ctttttgagc tgcaagtgca aactgacgga
    19381 tagcctgggc cacacccgcc atatcattag tcaaagtgac tttgtcagct aatggcttga
    19441 tagctgctga agcattttcc atagcaactc ctaaaccagc ataagttaac atctcaatgt
    19501 cattgggagc atccccaata gccatcactt ggtcagcagt taaacccaaa tcttctacta
    19561 attctttgag agccgatgcc ttgctgactc cctgagggag aatttctaaa atattatctt
    19621 ggctacgaac aacatgaaaa ctctgactaa gctgatttct caccgcacgt tcaaaggcat
    19681 ctagcgcagc tttttctcct aagtacatgg cttgaaaaat caggcgagga gtgtccgaca
    19741 gctctgctaa ggagacaggc ttgacaatgg taaataccaa atctccatct tcctgcacaa
    19801 ggtctggcac ttcttcttca agcaccaaat aatcgttttc ctcagttaaa gtcaggtaaa
    19861 tatctggaaa actttggctt agctcttcca gcaattcaat atctgaaacc ttcaacattt
    19921 ttgaatggcg taattgccag tcaggactac tataagtact acagccatta ttgataatga
    19981 ggaattcctc ttgtgtcaaa cctaattggt caaaataggg tcttgtccct gactgaggcc
    20041 ggcctgtgca taagacaatt tttaagcctg actgagctgc ttcttggatg gctgtgatat
    20101 tctcttttgg aatctgtttg tcctggttta ataaggtgcc gtccaaatca attgcaatta
    20161 gttgaatcat cctatctttc ctctttccat ttgattttag ggaggttcca ttttttacgg
    20221 ttgccaatca tgcgtaaagt cgtgagtaaa acaaccaagg cataacattc aatcgttgaa
    20281 tctgctagtt taaaatgcaa aacaatagct gcaagaatag accacccagc atagatttca
    20341 ctacgcaaaa cactaggttt tcgccctgcc agaatatctc tgacaacacc tccgcctgca
    20401 ccagtcaaca cagctgctac aatgacagca cttaaaggct ggttaagacg gacagcatgc
    20461 aaggctcctt ggacactaaa agctgccaga ccaatggcat cagtcaaaac agctgcccgt
    20521 acccaacctc tagctattag cttaggaaaa agcataatca aaaccatagc aaagaaagcg
    20581 caagtgaatt ctggcttttg cccccataag gcctctatag gtaaaccaat tagggtgttt
    20641 cgaatggctc cgcctccaaa ggctgtcacg aaacctaaaa taaaaatccc taaaatatca
    20701 aattcttctt ccatggcaac aatagcacca gaaagagcaa aggcaatggt cccgatgata
    20761 tttaaaattt cccacatatc aattgtcata agctacccta ttgtctttta gtcttctttt
    20821 agtttaccac gttttccttt aaaaaataag tagaaaaaga taaaatgtaa ggataaaaaa
    20881 gcaaggataa aacccatttt catccttgct acaaaagatt caaacaatta tccccatact
    20941 tatcttatta catcaatgag agctgcttga taggataagg ttgttttaga ttttttccgt
    21001 ccaagggcta gcggtctctt cgagtaccct gtttaactcg tcaatatttt ggaagccttc
    21061 agtgcatagc cactttcttg cttcgtcctc tcctttagta atataaactg gaacggatcc
    21121 tgcccacgtg gcacgcccac acaaaacacc gctaaattga gcacctgctg ctgctgcaaa
    21181 gtataatgtc tcttgaaaaa gtttggcaga tacgcctgca ctcaaataga tataagggag
    21241 gtggctggca gcttcttggt cttgaaaagc tttaatagcc tcagcttgac tgtaatggac
    21301 ttccccttca gtaaaacctt ctacataagc catattaaca ggaacctcta cttttagcac
    21361 atcaacgcca aaacgcttat ccgaaaatac tgacatagca ccgttaacct tatgtggctt
    21421 aagctttgca tatgccgcac tgttgttatc tgatattctc tcatcataac tcaaaagttc
    21481 aagaaaaaaa ggaatgtctt cagctgtaca ttctgaacca atgcgttcaa tataagcttg
    21541 tttttgtaaa ttgatttgtt catctccatc aacatcataa tagagcaaaa atttaatagc
    21601 atctgcacct gctgctttca accgtttgac cgaccattcc actaagcaat cgggtaaacg
    21661 actagtggtt gtagcatcat aacctgtttt ttcgtaagct aacagtaagc ctgtctgatt
    21721 agctcttacc ttagtagcca gcaacccata ttctggatct aataaaatag atgaagcata
    21781 aggcgtcaat tcctcagaaa cgagaacttt caatgtttct atctgtgtaa ctgtcgcttc
    21841 tccttcttga tgtgctgcca tcatttgctt cagtgcacct cgttgatcaa aagctaaagc
    21901 tgatatgatt ccttgatggc tcactttttc taagtaatgt cttttatttg ctgtgattgt
    21961 catgtgatac ctctcttact tgaagttgct gatataattg tttaaaagtt gccatagtca
    22021 tcaaatcttg ataatagtga gtagctagtc tcttaggcag actgcctgag aagttaagac
    22081 atcaaacggc ttagcaatag tactcatgtg gctcaaagag acttcggctt cgtccacatc
    22141 actgtaggga ccagcttcca aaatttttgt ctgcatgcct tcatgtaaaa cagcaataca
    22201 atttctcgtt tcccctttga ttttgaaaaa ttggttagaa atgccctgtt cttgtaactg
    22261 atgaatgata aagtcaccca gtttactacc actaaatcct gttgcaataa ctgaccagct
    22321 tttgttaaca ttctggtcac atttagctcc ttatcaccag ctgtttttgc cgttctgtca
    22381 acacgattaa ccgtgtccat agccagacaa tctaaaggat aggaaatagc gacagaagga
    22441 tttaaggtaa ctgttaaact catctcttct tcctaatcgt gatactcacc acggtcccat
    22501 ttttctaaaa attctgtaaa gaaatcagga tcttcttggt tagcatgatg agtttccaag
    22561 tgggctattt tagcaattag cttcttattc tcttcagttt ctttgtattc tgccttgata
    22621 aaagcgtcta taatatcaca cataagaagt tcaccagtga ttttaccacc aaagccaatc
    22681 acattggcat tcaattcttc cttggcataa agcgctgttg tcatatctcg aacaagggcg
    22741 gagcgaatac ctggtacctt gttcacggca ttattaatgc caactccagt tccacaaata
    22801 caaacaccca agtctgcttg accactaacc acagcttcgc cgactttttt tccaaaaata
    22861 gggtaatggg ttcttgtatg gtcataagtg ccacagtcaa taacgtcata gcctttagat
    22921 ttcaaaaaat cagaaacagc cattttttcg ttggtgacaa tatggtcaca accaatcgca
    22981 attttcatca ctttatcctc ctttaggcca ttttattgag catgtcaaca cgaatttgat
    23041 gacgaccacc atcgtaggga ccagtcacaa aacctttgac gatattcttc gctaattctg
    23101 gacctacaat ctctgcccct attgtgatca tgcgggcatt gttgtgacca cgcgtcatgt
    23161 aagcagagcg ttcatctgac acttcagcag caaccattcc ttttaatttt gtggctacca
    23221 taaagggacc tgctccgtaa gcatccacca taatgcctaa acgcccttct gcttcattaa
    23281 cggctttagc caccgctaat gtgttatcaa taaaatcact gttaatatca gtcacgtcaa
    23341 taatgtcata gccttcttct tgaagaaaac ttttgatcaa ttcttttaat gcatttccgt
    23401 gtgcatctgc acctaaaata atagccatcg tttctccttt tctaatccaa atcgcttgcc
    23461 aatctacaaa aagataacag gattacctat cattaggtca ggattattgc cagtaatctt
    23521 agcgtcttcc attatttctt tcaagacaaa gcacttgtgg gttcccacag agcgccttgc
    23581 cttacatgag aaatactctt ttcacgttag ggggtaaggc agaaatacct tacgcagtta
    23641 tctgctattc tccctaattt tttagtcagt cacctgacct gattttattt cattgttttc
    23701 gcatactcag cgttacgtgc cttcatttgg ttagcatacc aagcaaagag acctaggtaa
    23761 atggccacaa agatgattaa cgtgatgatt gaggcaatct gacctgttgt cgcttgacca
    23821 atcacatagc caaagatttt ttccattggt ccttcaagtg ttgaatgcgt aatcaaggaa
    23881 ccagcgcttg tacctgctgg aaaagcccct acttttttag ccaactctgt cgcaaatgga
    23941 gcaatcatag ttcctgacaa gaggaataat ggcaataaga gggaaccaaa aataatcatg
    24001 cgaatcaaac gaccacgtgt gaccacaaga agagctggtg tcatccccat agcaataatt
    24061 cccgccagtg gtaaaatccc attgccaact tttgatagga gaacggattc aatcaacatg
    24121 attggggcaa gaatattggc acaggcccag atttcagcgc gaccagcaat gaagggccag
    24181 tccaaaccaa tattgaaacg acgtccatgc attctagcat tggcaaaatt ggtaatccct
    24241 tgtgacaaag gctcaacagc tgcgataaac caagaaccaa tcaaagagaa aagttccaaa
    24301 caggcacccg cggtaaagcc tagaccaaac caatttttaa cagattcaac actaaaatga
    24361 ggatctccca taatcccaat cacaaaacct agagccattc cgataaagaa cttagagccc
    24421 cagaagccaa tttttttgtt caatttagca gcatcaaagt catatttatc tagccccgga
    24481 aagaccttgt caaaaatctt atctaagacc ataatgattg gattcatcat gtagttcatg
    24541 tgagttgaag tcattggtga ttgagaccct gtcccaagaa ggtcatcaaa ggtcggtttc
    24601 atcaaatcag agttaatgat tttcaatacc ccaacaagaa taactgccac agtagcaatc
    24661 aaaagagaca caaggactgg caaatggttg tgatcagcat accacataat caatagacct
    24721 gtgatagaca aatgccaaat atcaaaaata tcgacatcaa gggtgtccgt ttttttcatg
    24781 acaatcatga caatattaac caccagcatc accagcaaga aataaagggt ataaggggaa
    24841 ccccaggtaa ttgttgcaag cggtgcccaa ccgacatcaa taatattcaa actgattcct
    24901 gtatttttga caaaggcctg caaagactct gaaaaagccc ccgtcaaaat cccaataatg
    24961 gctccaatac ctgtaagagc aatagctaac ttaatcccgc cttccagtgc ctttgtgaat
    25021 ttaaccccga aaatcatggc taaacctgtc atgacaatca gcatgaccac acctgatccc
    25081 aactgaatag gaaatcccag aatcttgtta ataatgtcta aaaaaggttg catcatcttg
    25141 tctccttatg tttttattaa ctcactgcaa atggttttct ttaatcacgc gctctaattc
    25201 agcaaaaaca ggctcactca tgattgggat acgatagaga atcggtcctg cttctactgt
    25261 gggaattggc atctcaaatc ccaagtcagt ttttgcaatc ggcgtaaaaa tatcataacg
    25321 agacaccaat tcttctgtga tgtctttgac catcaccgca tcacaatgca catcatagcc
    25381 acgatcactc atctgagttt caatcgcatc tttaatttga tggcttgagt taacccctgc
    25441 accgcaggct gctagaattt taatcatgct gaaacactcc tttttctatt aaatagtttg
    25501 aaatagcttg tttatcatct tttagactgg ctagacagct aagattgcct ttcgtaataa
    25561 agaaggtaat aagattagat agaatcgtcg tttgaccctc gttttgacta ttgataataa
    25621 agaagaaatc cgttaccaaa cagtcttcct ctggattaat catgtgctta aaggtaaggg
    25681 cttgactatt tctaacatag accacttgat caaccaagca atacttggtt tctgtatgcg
    25741 gaatagccgc atacaaaata tctgacccat ctttgaggtc cactttgagt cctgtcggaa
    25801 ataccgcctc acgttccact aaagcctgat ggtactcttg cgtcacatac ccaacctttt
    25861 ccaagtggct agcaacaaga tcaaaaagct ctggctgcgt ccttgcttca gtaaatagaa
    25921 tattaggaaa cactccctac cgcctttcta taagtatcaa tttttaacaa ctgtcatctc
    25981 atcatcaaca aattggtaac cgtttacaaa atcattatag tataaccttc aacataattc
    26041 aacattttta cgcaatattt tttgttctat atgtttgttt ttgttttgtt tattttgttt
    26101 tattgaaata tgaaggtcta tatgctatga taaacataag taaataaact atagaaagga
    26161 gccccttaga tgttaaaaag agaaagactt ctaaaaatta ccgaaatagt caatgagcaa
    26221 ggtattgtta ctgtgaatga tattattcag actttaaacg tttctgatat gacagttcgt
    26281 agagacttag atgaactgga aaaagccggc aaattgatcc gcatccatgg aggtgcacaa
    26341 agtattacca tgcctaataa aaaagagcgg tcaaatattg aaaaacaaac cgttcaaaca
    26401 aaggaaaaat gggaactagc ttcatatgcg acacaactag tcaatgatgg tgaaacaatc
    26461 tttattggtc caggaacaac tttagaatgt ttcgctgagc aattaaaaaa tcgtcagatt
    26521 cgcattgtga ccaatagcct ccctgtcttt aacatccttc aggacagcga aaccattgac
    26581 ctgattctta tcggtggtga ataccgatct attacaggag cttttgtagg gtcccttgct
    26641 agtcaaaata tctcatcctt aaaatttgcc aaggctttta ttagctgtaa tggcatttat
    26701 aaaaacgata ttgcaactta tagcgaaact gaaggagaga ttcaaaagct agcttttaat
    26761 aattctattg aaaaatacct cttggtagat aaccaaaaat tcaatgccta cgacttcttc
    26821 attttttacc atttaaacaa cattgatgct gttgttacag attcgcaaat aactgaggac
    26881 gtcatcgaac gttatagtca gttcacacaa ttattagtag cccacgaaaa caaggagtaa
    26941 ccgtttcatc aagaatggac agcctgtcag cgcatcgtcc ttaaacacct tctggcaaaa
    27001 gtgatttgcc cccagagagg tgtttttgtt ttgctcggaa cacacctgct aacgtcagag
    27061 tctttatgag tgaccacaaa aaaagaagcc cagattaaaa aactggaacc tcttttgaga
    27121 tgaagtaagg ctaccaaaat aacctaagac catttatgta attcaaattt ggtatccttc
    27181 aaagcacgct tgagtaggaa agttaaagga cgtcctgtca ccttagcctc tcctttttcc
    27241 aaatttacct ggaccgattg cacaccgggg acagctgata atttttcagt aaccgttctt
    27301 gcacaaccgt cacacgtcat tcctgtaacc tgatagtgtt tttccatttc gttttctcct
    27361 tcaaatggtt tttcctttta atcgcagggc attcaaaaca actgatactg aactaaaact
    27421 catggcaaaa cctgctaaca tcgggttcaa tagtggacct ccaaaaagat gaagtaagcc
    27481 catggccact ggaatcatga ggacattgta tatgaatgcc caaaataaat tttccttgac
    27541 aatcctcata gtcgctcgac tcaatgacat agctttcacc aaatccagca tatcaggttt
    27601 catcagaatc acatccgccg attcaatggc aatatctgtc cctgacccca tagctatccc
    27661 aatgtctgct acagccaatg ccggagcatc attaatacca tcacctacca ttgcaacttt
    27721 tctcccttga gattggaggt ttgttatcac gcctgctttt tgatcaggta agacttgact
    27781 gatcacatct gtaatcccca cttgagaggc aatagcctta gcggtagcat cgtggtcacc
    27841 tgttagcatg atggtgtgaa ttcccagttg ctgcaaagct tcgacggtag cttgactatc
    27901 ttctttgaca gcgtcagcaa tactgaaaag agctcttagc tgcccctctt tagcataata
    27961 tacaagggtt tgacctttcc ctgaagcctt agcacaaact gactgaacag aagctaaatc
    28021 aataccaagg gttgccatta aagtagcatt tccaatcgcc aaacgacgcc cttcaagctg
    28081 accagtgact cctaggccgg ttaaagactc aaactcttcc actggtagac tatcgtaacc
    28141 agcttcctga gcagcacgga caatagcctc ccctaagggg tgttgagaat aggcctccaa
    28201 ggaagcagct tcttgcaaga taaggtgatc atctcccgaa aaagttagga ggttttgaag
    28261 acttggtttc ccctccgtaa tcgtccccgt tttatcaaaa acaaccgtat ctacctgatg
    28321 ggcttgttct aagatgtcac cacctttaaa aagaatacca ttttcagctg cacgccctgt
    28381 tccaaccata atcgctgtcg gggttgctaa accaagcgca caaggacaag caatgactag
    28441 aacagcgatg gctgttgtca aactaaaacg aagactttcc tgtatgaata ccaaccaaaa
    28501 cacccctgtt aacagagcta ataccaaaac caccggaaca aagacacctg agactctatc
    28561 agcaatggct gcaataggtg ctttattttc ttgagcattc tcaactagtt ggataatttg
    28621 tgctaacaag gtatcattcc caagcttatc tggtttaatc gtgagggcgc cttgaccatt
    28681 caagctgccc gcaaagacat tatctttttc tcccttttca acaggcaaac tttccccagt
    28741 taaaaaggat tcatccaaac tgctatgtcc ttccacgata atgccgtcaa tagaaacttt
    28801 ttcacctggt tgaaccagga caaggtcatc atagctaatc tcttctaacg gcaacaattg
    28861 ccattggctg tttctccata ccctcacatc agtagccttc aaggatagga gcttttgaat
    28921 agcttgataa gtacggccct tagaacgact ttcaaaatac tttcctagac caatgagtgt
    28981 caaaatcact accaccgatt caaaataaag ggagtgcaca aagtgcgccc ggcccagact
    29041 gatttcaacc actccataaa gactataagc aaaagcaaag ctggttgcta aagctaccaa
    29101 ggagtccata ttaggatgtc ccttgaacaa agctttgaac ccattatggt aataatgacg
    29161 atttaatact aagactggaa tcgtcaagag tagctgacta aagctgtaaa cgaaaggata
    29221 gcgatctggt tgcagaaatg aaggaagcca taagcccacc atgtgaccca tagctaggta
    29281 taatatagga atggtgaata gggcagacca caagacctga gaccaaataa cctttaggtc
    29341 ttcgtcacga cgacctgttt gcatgtgttt tgcatgttca tcaaagactt gtgcaccata
    29401 accagcagct tttaccgctt gaataacttt ggccgtttct aaatcgtcat cttgataagt
    29461 aaccgtgagt ttctcggtcg ctaaattcac aacagcctgt tctacttcag gcaattgatt
    29521 gaccgttttt tcaattgtta gagcacaaga ggcacaactc attccctcca ctaaaaatat
    29581 ctctttagcc attaatacgt ctcctttttc tgacagtggc actgcccagg caggcaatta
    29641 cacggcacct caagaactgc attttctttt ttgacttcta gtagctcttg gaatgcctca
    29701 atttcctcta aggtcatcgg taagtcataa agccgttcaa gaagtagacg cgtgtgttgc
    29761 cgctgacaaa taccgtctaa aacagtcgct agtgcttctt tttgtagcaa agtctcatcc
    29821 aaaagagctt gataaatata ggccctacct tcccgataag atgttaaaag atttttttta
    29881 actagcctac caatgagggt cttaatagtg gaatctgacc attgatattt tttccttaaa
    29941 atagtaataa tatcgctact tttaatatca ccactggccc acaccacacg catgacttcc
    30001 cattcggcag ctgatatctg ttgcataacg tcaccttcat tcacaatata tttacgtttg
    30061 taaattaatt ataacgaatt taactacatt tgtaaataaa taacttttct catttttcta
    30121 tccttatatt gacaaatgaa tcgtttttac tataatatta ttgcaataaa aataacattt
    30181 acattaagga gatacccttg aaacatccta ttcgaaaaac acttgtcacc ttaggtctat
    30241 tactgacctt atgcctgccg actccagttg cagctagttc tcgttcttgg aagagctggt
    30301 ttatcgagca atatttctgg ttaaaacgtg acaagagtta ctattctaaa caagatgacc
    30361 caagcttcca aagatacctt gatgcctgtc gtgaacaatc tgataaacct tatcaactag
    30421 atactaattt agtcaatggt ccccttgttc aagaaaatct ttatggcatg caggtttact
    30481 cttggaatga taatggaaaa cctgatcaaa aaacaattat ttatcttgcc ggtggttctt
    30541 atcttaacaa tccaacgaca taccatatta atatgttaaa gacattatcc acaagtcttg
    30601 acgctaaaat tgttttaccc atttatccta aagccccacg ttatacctat aactatacta
    30661 tgccaaaatt ggtcaatctt taccaacact attaccataa aaatcagaat attttcctta
    30721 tgggagattc agcaggtgga ggtttagcct taggtctagc tcatgcccta cacaatgaat
    30781 cagtccctca accaaagcag ctcgttcttt tatcaccttg gttagatgtt actatgtcac
    30841 acccagagat tccagaatac gaagatgctg atcctattct atcttcttgg ggattaaaac
    30901 gagtgggaga attgtgggct tactctgctg ataataccaa ccatatttat gtcagtccta
    30961 aaaatggtcc gatcacttat ctaccaccaa ttactttatt tactgggaca cgagagatct
    31021 tttatcctga tattcgcgat tatgccgcaa aactgaaagc cgctaaccac aatattacct
    31081 tcattaccca agaaggcatg aaccacgttt acccaattta tcctatcgaa gaggcaaaaa
    31141 cagctcaata ccaaatcatt gatgccatca acaaaactcc ttaatgaggg gaataactcc
    31201 tacctgccac cagttaatct ggtggttttt ttgtagtttg caccacaaaa taacaattaa
    31261 aaagagccct atcataatga taaggctctt tctgtcataa catccgcacc gcaatactta
    31321 ggatttgttg tcaagatctc gtaagagttg atcaatttta ttaccatagg cgatggaatt
    31381 gtctttttca aacaccaaat ctggaatttt gtacatggtc aattgtttgc caagttcacg
    31441 tttaatcgtt ccagtcgctt tttcaagccc tgtttgtgct ttttgattat cagatgccaa
    31501 gtcactcata atagtgtaat aaactttagc aagtgacaag tcaccctgca tttgcacttc
    31561 tgtaatcgtt actccctgaa cgcgtggatc acgcactttt ttctgaagaa tatcatttac
    31621 ttcgcgcttg atttccatcc caacgcggtc aatacgatga ttagccatag ccatttcctt
    31681 tccacatttt taaaacaaaa gctaggctga aagcccagct tatgacattg ttacctgttt
    31741 tgatagcgac aattaaaggg aatctgagaa ttccatgacg agactggaag gaatgcccca
    31801 agtagctcat ctggtagaaa aagtcattat ttccgacaga ggaaaaacgt tacatctcag
    31861 accttcttag cccctatttt ctcacaattt cttccataat ataggcttca atagtatcgt
    31921 caactttaag atcgttaaag ttttcaatca tcaacccacc ttcttgagca ttaccgactt
    31981 ctttaacgtc atctttgtaa tgtttcaagc tggccagttt accatcaaaa ataacgacac
    32041 tatcacggat aacacgaaca ctcgaatcac gtgtcacctt accgttgatt accataaagc
    32101 caccaattgt tcccacttta gaaactttga aggtttcacg aataatggct tcaccaagaa
    32161 ttttttcttg gtaaacaggg tcaagtttcc ctttcatggc ttcttctact tcttcaatga
    32221 ctttatagat aatgctatga agacgaattt ccacatcatc cgtatctgct tgttgacgag
    32281 cttgtggggt agggcgaacg ttgaaaccga taataacagc atttgatgct tcagcaaggg
    32341 tcacatcaga ctcattgata gcacctacag cagagtgaac gacattgaca cggacacctt
    32401 ccacttcaat ctttacaaga gatgctgcaa gggcttcaac agaaccttga acgtctgctt
    32461 tgatgatgac attaacagtc ttgatttctc ctgctttaag ggtatcaaag agattatcaa
    32521 ggctaacacg ctgtgtattt tgacgttgtt tgaggagggc acgttttgat cgctcctcac
    32581 cagctgcacg cgcagctttt tcatcagcgt aaacagcaaa gtgatcacca gccattggtg
    32641 tttcgttcaa accagtaatg gaaactggtg ttgatggctc cgcagattta acacgacgcc
    32701 caaggtcatt taccatggca cggacacgac caaaggtatt accaacaacg attgggtctt
    32761 gaacatgaag agttccttgt tggacaagaa gtgtagcaat agcaccttta cctttatcta
    32821 agcgtgcttc aatcacagta ccaatagcac gaaccgttgg atcagctttc aattcttcta
    32881 cttcagcaac caaaaggact gtttctaata gttcatcaat atttttatta aatttagcag
    32941 atatctctac aaattcgcac tctccgcccc aagcagttga gatgatacca tattctgcca
    33001 attctgcgat aacacgttct ggattagcac ctggtttatc aattttgttg atagcaacaa
    33061 taattggaac accagcagct tttgagtgat tgatcgcttc aatcgtttgt ggcataacac
    33121 catcatcagc tgcaacaatc aaaatggtga tatcggtaac ggaagctcca cgtgcgcgca
    33181 tgcttgtaaa ggccgcgtgt ccgggagtat ccaagaaggt aattttcttg ccagcttctt
    33241 caatctggta agcaccaata tgctgagtaa ttcctccagc ttctcctgtt gccacacgtg
    33301 agttacgcaa agtatcaagc aaagttgtct taccgtggtc tacgtgtccc atgatagtta
    33361 caacaggtgc gcgttctaca atattttcag gattgaggta attttcatcc tcaaagaagc
    33421 gctcgatatc agcatcatct acttctacct tagccttagc ctcaatacca taatctacca
    33481 tcaataattc gatagtatcc ccatctaaag actggttttg agttgccata acacccatca
    33541 taaacagctt tttaacaatt tcagcgggtt cgcgtttgat acgttttgcg atttcgacaa
    33601 ctgtcatgcc ttcagtatat tcaaattctt ttggcaattc atggaattta cgctcggtta
    33661 ctggttttgg agcggtgttg gtattcttaa catttttgcc ttttttggtc tttttatttt
    33721 tattccagtt actatttttt tgatttctca cttggttttg actattccaa ctcttcttat
    33781 ttttcttttg tccttcttgt gaatgatgag ttaactcacg attcttttca ggacgtgctt
    33841 gttttttacg gcgattatca gcagtagatt tagcattaat atttgatgac tttaatttag
    33901 tttctgcttc ttcaatagat ttcagtgctg cttcacgatg gctcatctcc tcagcttgtg
    33961 cttttagagc cgcttcttta gcttcttgtc ttgccaactc agctaaacgc ttagcttctt
    34021 gctgtgcacg aaaacgtgtt tcactttggc gtgagtattc agcattttgt tcagctttta
    34081 aggcagcagc acgagctttg aaatcaattt taggactttt cgattgaggc tcttgtcgtc
    34141 cttgcttttt ttggtttgtg aatcgaccat tcttgtgatt ctccttagca aaggaacggt
    34201 tacgatcacc aactagctga tgattatcat gtctaggtga tacattcgat gcttttcgct
    34261 caatacgctt atcgtttgta tcaccttgag aacgatgagt aatcctctta tctccttgtt
    34321 cgttagtttg atgattattg ggctggcgat aacctcctcg acgttcttga gaacgatgag
    34381 aagagccatt tgcttgtttt cgagcagcct gttcctttgc acgagcctct ctctcagctt
    34441 taaaattacg actctttggc ttagagacct gtgttttctc tacatgctca gaacctgttt
    34501 ctttgaccac tgttacagag ggttgagcaa cttctttggg tttgactgtt tgagaagctg
    34561 tcacatcagg tttagaagcc tttgaaaaac ttgaaataat ctttttcgca tccgcttctt
    34621 caacacttga tgcatggctt ttcacatcta atcctaagta tttagcgtgt tcaaccacct
    34681 ctttactact tttaccaatt tccttggcaa tttcatgtaa tctttttttt gacaatttgt
    34741 gtcctcctat tcatctattc cataagagtc ctcattttct ttgaaaatcc agcatctgca
    34801 atggcagcaa ccttccttgg tttacctagt gcagcactta attccagtgc attcaacact
    34861 gtggagactt ctacattata ataattactt ttatctgtta ctttttttgt cacattagga
    34921 ccagcatcat tggctaaaaa tactaatatt acctgctggt gttgaatagc ttttactact
    34981 agttcttcac ctgaaatcac ttttccagcc cgttgagcta gaccaataag actagataat
    35041 ctttctaaat tagtcaagac ctaactctct tcttttgatt ttgtgatcaa cataagcaat
    35101 caagtcatcg taaaaacttt ctggaatgtc cattgagaaa ctcctgttga aaacttgttt
    35161 tttcttggcc attaaggcct cttgattgtc taatttgata taagcaccac gaccattttg
    35221 tttaccagtt ggatcaatga aaacttgacc atccttagtt tttacaatcc taagaagatc
    35281 tcgtttagca ataatttcac cagaaacaag tgattttcgt aaaggtattt ttttgacttt
    35341 agacatcaaa cacctctttt cttatacttt ctcggcagtc ataacatcgt caacaactag
    35401 ctcttccaca gctgttgctg cttctttttc cgcctcaaga cgatcatatt cgctagctga
    35461 tttgatatca atacggtagc cagtcaaatg tgccgctaaa cgaacatttt ggccgcgacg
    35521 cccgatagct aatgataatt tgctatcagg aactactacc gtagcacgtt taagatcttc
    35581 atcgtcaaaa agtaccatgt ctacttcagc cggagcaatg gcattgtaga tgaactcagc
    35641 tggatcatct acccattgaa tcacgtcaat attttcttca actggaattt caagccccgt
    35701 cttagcatct acacgttttg gatggaactt gctgatgacc tttttaatat tacttccacc
    35761 acgtccaaca attgttccga tggcatcaac gttaggatta tgactgcgca cagcaacctt
    35821 ggtgcgatca cctgcttcac gagaaacact catgatttca acagtaccat caaagacttc
    35881 aggaatttct tgctccataa tacgtttgat gaattctgga tggctacgac taacaaagac
    35941 gttaactcct ttaggattat tttcaacttt gtagacataa acgtcaatac ggtcatgtga
    36001 tttgaaagtt tcacctggaa tctggtcttg gtgagacaat tgtgcttcta aggaaccaag
    36061 attgacataa ataaaacgtt gatcaaaccg ttcaactgtt cctgtcataa tttcaccttc
    36121 atgctctttg tattcattga acatcacttc acgcatttgg cggcgcatct tttccataat
    36181 agtctgttta gctgattgag ctgcgacacg tccaaattca ttgacagatt cttcaaagcg
    36241 aatcttatca cccaattcat aggcagaact gatagcaaga gcatctttca aactaatctc
    36301 aagccggctg tcaaaaactt cctcaacaac ctcacgaacc gtgaatactt gaaaatcagc
    36361 tgttttttca ttaaattcaa taacacatga ttctgattga ccatagcgac gtttataggc
    36421 agactttaat gattctgtca ctgcgtcaat aatatctgct ttatcaatgt gtttttcttc
    36481 ttccaaaata cggaaggctt ctagcatttc tttgctcata atgttatcta ccttgcttgt
    36541 aaagcttggc aaaaatcctt tcttaaatta atacgatcaa tagcccccta aaggctccct
    36601 taattccttt tgtttagctt gtataaactc tataggtgtt gtttcaaatt tgcttaagcg
    36661 ctcctaaaaa agtttagtgc tttgaaataa aagttataat ttgacagcca ttctcacctt
    36721 agcaactgct tgatatggta tattcacgat cttatgacgt gttttatcaa gataatcaat
    36781 ggtcaacgtt tcaccatcaa aggctaataa gtctccttga aaaactttca ccttatcaat
    36841 agcttgataa agactcacat tgatataaga gccaactgct gcttttaaac tgtcagctgt
    36901 cttaagcgga cgctccaaac ctggactaga gacttctagc atatattggt ttggaaaagg
    36961 atctggatca atcgtatcta acagcggact aataatattc gtcaagtcag atgtatcttc
    37021 cactgtgatt ccaccttcct tatcgactaa aatactaagg atgtagtcac tacccatttt
    37081 gtcatactca acgtctacta attcataagg tgccttaatc tcaggcgtta ctgtcttggt
    37141 tacgatatcg ataatagatg tatttgctat tttgattacc tcctctattt ttatgctctt
    37201 ctctaagata attggtcctt gggaatccac ttaatacaaa agtggcgaga catatcccac
    37261 cacctttctg tattatttaa gcctattata ccataattta agaggaatgc aaggatatgc
    37321 actttgtttt actcacaagg agaggagggg attcgaaccc ccgagccccg ttaaggacta
    37381 cacgctttcc aagcgtgcgc actaagccac tatgcgacct ctccattact aaagaagtta
    37441 atactgtatc aactccttta tgttatcata ttttgaaaca aaatactact agaaatttgc
    37501 ctcaactcgg taaatgactt gacctttatt agagaacttc tcttcatatt ctgtcatgac
    37561 atttccttca tagttactag cgtgtaagtc aagccagatc tgtctcaagg tcatgccgta
    37621 ttgagaaaaa ctagctaaac tgtactcaaa caagccccga ttatcggttt taaaatgaat
    37681 ttcaccgtgt tctggtaaga tacgcttgta agtgtctaag aaatccttgt aggtcaaacg
    37741 acgtttttca tgcttggttt ttggccaagg atctgaaaag ttgaggtaca tcatatccac
    37801 ctcaccatct tcaaaatagt tggttaaact tgatccatcc actcgcagaa gtttgacatt
    37861 gggtacttct gaagctaata ccttatccaa ggcgtagctt aggacagata attgaatatc
    37921 aatgccaatg tagttaatat caggattttt caaggccatc cctgtgataa atccgccctt
    37981 tcctgaacca acttcgatat gaataggacg gtcatttcca aaaacgtcat gccaacggcc
    38041 cttggcatct tctggattta atatcacata gtgtgggtta ttagctaagt gctcctcagc
    38101 accttttcgt tttctaactc gcattatttc tttctaaata tttctctaaa ttttctgagg
    38161 gcataaatct cctgattcac atgctccatg tcacgcttgt caaaacactt gagaatttgt
    38221 gtcaggtgag aaaattgacc ataccaaata attttttgca taaccttgtc attattttta
    38281 tagccataat aagacagcca ttctgaccaa cgagaccgtg gaatatagtg gctcaacagg
    38341 taagcaacat catacatccg atcagtcaga cgaacagaat cccaatctac taaaaaaatc
    38401 ataccgctag tagtaatcac ccaattgcta tgtttaatat ctccatgcac aatcgttgct
    38461 acttctgatt tgaactctgg taagctccgt tttaattctt taacgatagc ttgtaagtat
    38521 gaattttgct gaatttgcaa gggtgcattt tgctcaaaat caaccaataa atcgtatggg
    38581 ttttcaatct tataattgag ctgaagcagt tgattgacta attttttaga tttgtgaagg
    38641 cgcaatagaa tatgaatgat ttgcttactg ttcatatctt ctttggtcaa tgtacggccg
    38701 ttaagccatt cttgggcact catcatatca ccattgccca tgcgtttggc ccaaagtaac
    38761 tgtggcgcaa tctgttcttt tgctaaggca ggtagaatag gggtcgtatt taattttata
    38821 aagacacatt ccccatttgg ataagtgcct ttataagctt tgccactttt cccacgtaag
    38881 ggagtcaagg taagttcttg ttccgtcgtt gtcaccttat cgccctcctt ttctatcttt
    38941 tatagtcgtt tacccctatt ttacttgttt taggctactt agtcaatgat cttctttaac
    39001 ttataaggta gatatataca agctatgaaa accataccta caatcaaaat gagtgctttt
    39061 ggcgctgatg agcaacacag catattgacg attaacatta aaaagcttaa acctcgtaaa
    39121 aaacttaaca catttttctt cttgctccga ctattttcag gataaagact agtcatataa
    39181 tggtaatcgt agtgataata aagtgaaagt aattggaata caactaaata gttaaaaatg
    39241 agtgctaaac ttactgacaa ataaagatta tggataaaaa atacagataa tgagcttaat
    39301 agcattagcc gtaaaaataa gcccaaataa tctgaactac gtaaaaaggc tctagcataa
    39361 agggacagcc aaaggtttga aggagtttgc ttaactaatt ttaaaagagg atttagataa
    39421 gttctttctt tcacttttgt agaaattcct ttgactgttg taaaaagaga ataaaacttt
    39481 aaaatacttt gcttgcgatt gctttcaaat gcgacagctt ttgcccaatc taaacgattt
    39541 cctctcaaaa aatatcttgt ctttcgacta agcactactc tttttagtgc caataatatc
    39601 aaaactaaga taataaaaat aaataaagac agacctagac gtctaaaaat aggatataaa
    39661 aagagcaata cagcagtctg caaagttccc caaagcaaaa aagagagtcg ttcagcttgt
    39721 ttgatataag aaatcacttc ttcttctttt ggaagtaaga aatgctgatc tgcctcttct
    39781 agatacgttg ctatagatcc catactgagc aataatggga tcaatatccc aagacaaacc
    39841 tgaataggta gatgattcgt tggaaaatgg tttagtagct gaccatactg caccatagca
    39901 aagcctaata aaaacatcaa caccaaaaca aagtgatcat ttaagacgta acgaaggtat
    39961 ttattttgct gtttttgaaa gtcttgacgg cgtttcagaa ataatgcttt catacttaat
    40021 cctcttttgt taatgcaaga tagatatcat ttaagctcgc tgaacggtca ccaaaagctt
    40081 cttgcaagtc agcaagagtt ccttgggcac gaacttgtcc atgatgcaaa atcacaaaac
    40141 gatcacacat tctttcagca gagtccaaaa catgtgtact catcaaaata gattttcctt
    40201 ttgctttttc gacttctagc gtttggatta agtctgaaat agctaaaggg tctaacccta
    40261 agaatggttc atcaagaata aatagacttg gatcaatgac aaaggcacaa ataatcatga
    40321 ctttttgttt catcccttta gaaaaattga caggaaacca ttcaagtttg tcagttaaac
    40381 gaaacatttc caagaaaggt tgtgctcgtt tttgtgccac ttctaagtcg atatcataag
    40441 ccatagctac ggtgttgata tgctctgata aggtcaattc ttcatagaga ctaggagttt
    40501 ctgggataaa acctattttt tgacggtagg caactgcatt ttctgctaga gttaaaccat
    40561 caatagaaat acttccttga taaggcttca aaaagccaat aatttcgttg atagttgttg
    40621 actttccagc accatttagc ccgatcaaac cgactaactc tccattgtct acagaaaatg
    40681 agacatcgtt taaaacaggg atattgtgat atcctcctgt tagattttta atgtttaaca
    40741 tataatttct ttctagtttt tgatatactt ataatatatt ataacaaatt caagtagaga
    40801 aggtgatata aaatggaaaa ttgtattttt tgcagcatca ttcaagggga tatcccctct
    40861 tcgaaagtct acgaagatga gcaggtcctc gcctttttag atatctcaca aactacaaaa
    40921 gggcatactc tagtcattcc taagcaacat gttcgtaact tactagagat gactgctgag
    40981 acagctagtc atctctttgc tcgtattcct aaaattgctc gtgctatcca gtcagctact
    41041 ggcgctactg ccatgaatat cattaacaat aacgaagcct tagcgggaca aactgttttc
    41101 catgctcatg ttcatctcgt tcctcgttat aatgaagaag acggtatctc aattcaatac
    41161 accactcatg agcctgactt tcctgtcttg gaaaagctcg cccgacaaat caatcaggag
    41221 gttagttcat gaaagttaaa accatgatag ccgtcggatt attatctttt accgcttaca
    41281 aagcatatca aaaacgttgt accataaaag agctattatc catcagtcga caagcaaagg
    41341 atgctgcaca acttgattta gataacatta aagcaaacct tgacctcatt cacagtcaag
    41401 gaaaagtaat tcaaaatatt agtcaggact tggcccataa atggcgctac tttaaccaag
    41461 agacacaagc acacctcact gaaatccaaa atcgtatggc caaatatcaa ggagatagtg
    41521 agctaatttc tagtgaatca cctacttgcc aaaccgtcat tttaactaac aaccattcca
    41581 gatagtatta tctccctcat atactttatt tgaaatcaca gacagctatc atctttgata
    41641 cctgtctttt ttatattcaa aatcggatat tttaaacata aaaaccctca gaacgttagg
    41701 tctgagggtt gatattagca ggaggaacta atgatccaga ccctgatgag ctagactcat
    41761 cagctgctaa agcatctgat gaagaagacg gcgtagttgg cgtatggctt gaagcagtag
    41821 agtcctggtc tgtactagca ctctgataag atgagccggt atctactcca cttgaataat
    41881 tagcgtaaga gctatgacta tcagaataag atggagcctg gcctgaagag tcatagttgt
    41941 tgtttactgt ctgagactta gttgacccat acaaattttc ataaacagta gcatttgttt
    42001 ttaattgact aaccttatga agtcctaatt ctgttcggat acgattttgg atttctaaca
    42061 aatgattaga ggtaacaatt tggtatgatc caccatctga taaagtggca tcttctcctt
    42121 ttagttgata agtcttaata gttctaagtg cgtcacgata acctaataga ctagggatag
    42181 tgcgagaaga gatttcgata ttcgtttgca tattactact tacagcagat aaaatcttcc
    42241 gataagagct aatgctatca agagcaagga tttttttcaa taccttttga atgacttcac
    42301 gttgacgctt ttgtcgacca taatctccct caggatcatc ataacgcata cgagcataaa
    42361 ccaaagcttg ttcaccgtta attttgtgtg ttccaggcgc aacagtagct tgatattcag
    42421 gttcgttttc agcaatcgag ataggaaaat caaactcatt tgtaactgta atccctccaa
    42481 ctgcattcac aagatcaata aggccttgca tattaatttg aacatagtta tcaatggtga
    42541 tattcaaaag atcttgcacg gtcataatag ccatctgagc gccacctgct gcataagcag
    42601 cgttaagctt agcttcaaca ccattcattt cattattttt gggtccagat aacgtggtta
    42661 aggtatctcg ttctaaacta gtcatagttg ttttcttggt ctttggatta accgtaacca
    42721 aaatcatcga atcactgttt ccttcccact tggaggcacg ctctgaagag cctgtatcaa
    42781 cacccatcaa caagatagaa aaagctcttg tttgtttaat ggcatcactt ttctttgaag
    42841 atgtcgaaaa atctttaaag gtctttgata attctcctgt tgagaaggta taagcactag
    42901 ttagatagac acctaatgcc aagacagttg ttaacacaat agctgtgaac attaaaacta
    42961 ttttttttcc aattttcatc gattcactta ctgacttaca gatttacgca tatgataaac
    43021 atctaagtca tctccatttt ttgatttgat gtcgtttttt cgcatacttt caatacgaaa
    43081 accatacttt ttatacaggt aaatagcctt agtatttctt acttgaacgt ccaacttaag
    43141 gctttcgata taagggtttt cttcagccca atcaagagca atctctaaca ataattgacc
    43201 gataccataa ccacggtaag tcttagcaac taacataaat acatctgctt cagcttggcc
    43261 ttgggataat acttccccag aaagatttaa aagccctact actttttcat ctaatctagc
    43321 tagcaggcaa aaatcaataa aagaggttcg acttcttgat aaaaagtctg acaattcttg
    43381 aggagtagcc tctaatatag tctctacgcc tgttataaaa tctgtttctt tggcagctgc
    43441 cgtcactaag gaaagtacag cttttgcatc ttgaggtacg ccttcttcaa taacgagact
    43501 ttgctgaatc atgtgacatt atctccagaa gctgacgaga gcgctcacca tgcgctaaca
    43561 agtctagttg acgacctttg tctcgtttag taatggtaat ttgcaaatag tcttgtaata
    43621 atccctctcc taataattct ccccactcaa taacggtaac accattacca aaaagaaaat
    43681 catctaaatc aatagagtct ggatcatcac caacacggta aatatctaag tgataaagag
    43741 gtagacgtcc ttcatattct ctaacaatag tataagttgg gcttttgatc atttgactaa
    43801 tccccatccc tttagcaata ccttttgcca atgttgtttt accggcacct aagtcgccag
    43861 acaatacaat cacatctcca atagacaaat aagtacctag ggtttcgccg taagctttaa
    43921 gagtatattc attttcacta taaaacatag caacattata tcagaaaaaa aacatacttc
    43981 caagcactac ctagaagatg actcaaataa ggtattcgta aagtaaaaac gcttctttat
    44041 attaaaggtt aaaatcaatt aaagaagcgg cagttagtag caaaataagt aaattatttg
    44101 gttataagat agttaatatg atgaaattta caatgaacaa gaaggttgct ccccaaataa
    44161 tagggtgaat atcttttgtt tttccctcaa caacttttac tagacaatag aaaataaagg
    44221 cagcggcaat accataggaa atagagtaac acagcgccat aaagaaagct gcaaaaaaag
    44281 ctggaagagc atctgcaaat ttactccaat taacatcaag aaaagaagac accatcatga
    44341 cacccacaac aattaaagct ggagccgtag cagcagccgg gacaatacct acaagcggta
    44401 atagcaatat tgataataag aagcatactg cggtggagac tgctgttagt ccagtacgtc
    44461 caccttcagc aattcctgct gctgattcaa cataggtagt cgtatttgaa gttccaacca
    44521 aagccccaat agaagtaccg atagcatctg caaaaagcgc acgatccatt tttgaattaa
    44581 agcctatact attttccaaa gcattctcat cgtcttgaga gaaaatacct gttcgacgac
    44641 cagttccgat aaaggtacca attgtgtcaa aagtatctga tagactaaaa gcaaaaatag
    44701 tcatgaaaac tagcggtaaa cgacttgaat cgctaaaaag agaactcaaa ccatcgaaag
    44761 ctgcaaggaa agttgtacct aattcagtcc aagcttcacc aatatggctg atcccaaaat
    44821 tgaggttgga cacatccact attcctaaag gaatacctac aagagttgtt gcgacaatac
    44881 caattaaaat agcacctcta aaatttcgaa tcacaagagc tgtcgttaaa agtaaaccaa
    44941 aaatagcaag taatacactg gaatccgtaa aactagaaat cgtaggtaca actccaccat
    45001 tggcgtctac aaataataga ccatctgcaa atgttttagc cgatgcttta gccggttcaa
    45061 caccatttac cattactata ttttcagcag agatagaaaa agtaatgata tttgcatttt
    45121 taaatcctaa ataagctaca aagacaccaa ttcccccacc aatagcatgc tgtaaactaa
    45181 ctggaatcgc cttgatgata cttttacgaa acttggttac ggtaataaaa atattgaata
    45241 atccacaaat gaaaaccatt gccaaggctt cttgccatga aaaccctaaa gcaaaaacaa
    45301 ctgtataagt gaaaaaagcg ttaagtccca ttcctggtgc caacgcataa ggcacattgg
    45361 caaataatcc cataattaag gttgatatgg ctgctgcgat aatcgtagcc aaaaagacag
    45421 cattagaggg catccccgct gcacctaaaa tacttgggtt aacaaacaaa atataggaca
    45481 tggcaaaaaa ggtcgttaaa ccagccataa tctcagttga gacagttgtc ccattttcgc
    45541 ttaacttaaa aaacttttcc attgaaatgg agtctcctta attaaatagt aggtaaaatc
    45601 aaaaagcgct tatcactaga taagtctttt tgacacctca ttattattgt tattgttcgt
    45661 attaaaatga acaataacac tatgttacca tttttttgtt cacttttcaa cgtctatacc
    45721 ttcatttatt cattaaaaaa acagttatcc tatctttttt actaatgaat acgcttaact
    45781 tatcttttct ctttatcttg gagcaaaatt tgctataatc taattatgac taaaaaattg
    45841 attgccattg atttggatgg aactttgtta catcatgaca acaccatctc tacctatact
    45901 caaaaaacca tcaaagctgt ccaagacaag ggccaccacg tcattatttc aacaggccgt
    45961 ccttaccgca tggcactggg ctactatctg cagctcaatt taaagacccc aatcatcact
    46021 tttaatgggg ccttgaccca catgcctgaa caaaaatggg cctatgaaca taatgtcact
    46081 ttggacaaag gctacctact aaggttacta aaatatcagg atgatttcca gatggatttt
    46141 attgccagtg agtatcgaaa aaatgtctac atcaccatga ccaatccaga aagtatcgac
    46201 ccgcaactct ttggtgttga tgaaattact caggacatgg cattagagat taccaaaatc
    46261 actcgaaatc caaacgcctt attgatgcaa acgcgccacg aagacaagta tgccttagct
    46321 aaaaacatgc gtgctttctt caaagatgaa attgaaattg attcttgggg aggccctctc
    46381 aacattcttg aaatttcgtc taagaatatc aataaagctt acgctctgaa ctatctcctt
    46441 ggaatttaca atatggataa aaaggattta atcgcctttg gtgatgaaca caatgacact
    46501 gagatgctag cttttgctgg aactggttat gccatgaaaa atgctagccc tgtgctactg
    46561 ccttatgctg atcagcagct taatttttcc aacgaagaag acggtgttgc caaaaaattg
    46621 gaagaacttt tcttatagta gcaaacgaaa ccactatttt tcagaaaatg gtggtttttc
    46681 ttctgtcaac agctatcaaa aaatgaaaat cattttaata atttcaacat ttttcagcat
    46741 atttttgttt taaacagctt aaaaacacct ttaatatcaa taatctgcct attcaatcct
    46801 aaattctgaa aattttttca tgttttgtac tatttttccg aaagggcttg attttttagt
    46861 gaaaacgttt taaaatagaa acgttcttga atttcgttta agtgaaaaag aattgccaaa
    46921 atttagtttt ggaaggagat tattcttctt catttcaacg aaaaatattt taaaggagga
    46981 agaacaagaa tgggtatcgg tattattatt gccagccatg gtaaatttgc tgaaggtatt
    47041 catcaatctg gttctatgat ttttggtgaa caagagaaag ttcaagttgt tactttcatg
    47101 ccaaacgaag gaccagatga tttgtatgga cacttcaaca atgctatcca acaatttgat
    47161 gctgatgatg aaatccttgt gctagctgac ctttggagtg gttctccatt taaccaagct
    47221 agtcgtgtgg caggtgaaaa tcctgaccgc aaaatggcga tcatcactgg tctcaacttg
    47281 cctatgctta ttcaagctta cactgaacgt cttatggatg ctggtgcagg tgttgaacaa
    47341 gttgctgcaa acatcattaa ggaatctaag gacggtatca aagcccttcc tgaagacctt
    47401 aacccagttg aagaaactgc tgcaactgaa aaagttgtta acgcacttca aggagctatt
    47461 cctgctggaa ctgtcatcgg agatggtaaa ttaaagatca atcttgctcg tgttgacact
    47521 cgtcttttac acggtcaagt tgcaacagca tggactccag cttctaaggc tgaccgtatc
    47581 attgttgctt ctgatgaggt tgctcaagat gaccttcgca aacaattgat caaacaggct
    47641 gcacctggtg gtgtcaaagc taacgttgtt ccaattagca aattgattga agcttctaaa
    47701 gatcctcgtt ttggtaacac acacgcactt attcttttcc aaacccctca agatgccctt
    47761 cgcgcggttg aaggtggtgt tgaaatcaac gaattgaacg ttggttcaat ggctcactca
    47821 actggtaaaa caatggttaa caacgtttta tcaatggaca aagaagacgt tgctactttt
    47881 gaaaaattac gcgacctagg tgttacattc gacgtccgta aagttcctaa cgattctaag
    47941 aaaaacttat ttgagcttat ccaaaaagct aacattaaat aagtttcaaa cgctataagg
    48001 ttttaaaacg atgactagct aacttattag caaaaagtcc tcgtttacta tactttgaaa
    48061 ggattacaac atgtcagata tttcgattat ttctgcaatt ttggtcgtta tcattgcctt
    48121 cttcgctggt cttgaaggta tcctcgacca atttcaaatg caccaaccac ttgttgcctg
    48181 tactttgatt ggacttgtaa ctggacacct tgaagctggt gtcatccttg gtggtacact
    48241 tcaaatgctt gctcttggtt gggcaaacat cggtgctgcc gttgcccctg atgctgccct
    48301 tgcctctgtt gcagctgcta tcatcatggt caaaagtggc gactttactc aaaaaggaat
    48361 tacctttgct tactcaacag ctatcccact tgctgttgct ggtcttttcc ttacaatgat
    48421 cgttcgtact ttgtcaactg cccttgtaca cgctggtgac aaagctgccg ctgaaggtaa
    48481 tttcgcaggt attgaacgct tccactttat tgcccttctt cttcaaggat tgcgtatcgc
    48541 tgtcccagca gctcttcttg ttgctgttcc tacttcagcc gtacaatctg ttttgaatgc
    48601 tatgccaaac tggttgaacg aaggtatgca aatcggtggt gcgatggttg ttgctgtagg
    48661 ttatgctatg gttatcaaca tgatggctac tcgtgaagta tggccattct tcgccctagg
    48721 ttttgctctt gcagctatca gccaattaac ccttatcgct atgggtgtta tcggtgtagc
    48781 aattgccttc atctacctta atctttctaa aaaggggggt aacggtggta atgcagcagg
    48841 ttcagctgat ccaatcggcg acatcttaga agactactag aaaggggtaa gaacaaatga
    48901 ctgaacaaat taaattaact aaatcagacc gtcaacgtgt ttggtggcgt tcacaattct
    48961 tacaaggttc ttggaactac gaacgtatgc aaaacatggg ctgggcttat gcacttatcc
    49021 ctgctcttaa aaaattgtat acgtctcctg aagaccgtgc agctgctctt gagcgccaca
    49081 tggaattctt taacactcac ccatacgttg cagctccaat cattggtgta acacttgccc
    49141 ttgaagaaga acgcgcaaat ggtacgccaa ttgatgacaa ggctatccaa ggggttaaaa
    49201 tcggtatgat gggacctctt gctggtatcg gtgacccagt attctggttt acggttcgtc
    49261 ctatccttgg ggcacttggt gcttctttgg cttcaacagg taacatcgtt ggtccacttc
    49321 tgttcttctt tggatggaac cttatccgta tggccttctt atggtatact caagaatttg
    49381 gttacaaagc tggttctgaa attactaaag acatgtcagg cggtatcctc caagacatta
    49441 ctaaaggtgc ttcaatcctt ggaatgttta tccttgcagt gcttgttcaa cgttgggtat
    49501 ctatcaactt tactatcgac cttcctggaa aacaattgtc agatggtgcc tatgtggtat
    49561 tcccagatgg cgctgttaaa ggtgctgagt tgaaaactat ccttgccaac gctattggtg
    49621 gtatgagcct tgataaagtc caagctcaaa cccttcaagg acaattggac tctcttatcc
    49681 caggtttagc tggattactc cttacattcc tttgcatgtg gttattgaag aaaaaagttt
    49741 ctccaatcgc aatcatcatc ggcttgtttg catttggtat ccttgctcac cttgcaggta
    49801 ttatgtaatc aaccaatgga agtctgagat gaactcaggc ttctttttta tgctttatgt
    49861 caggaaaatc ccacttaaaa tatggtataa taactacaac tacgcaatga aaggaactta
    49921 aatggctcaa tctttaaaca ctagtgtcga atataagaca aaagctgtct cctaccttgg
    49981 aatgggaggc aaagttggtc atattttact cggtgacaag gcccttgaat tttacaacga
    50041 taaaaatgtg aatgattata ttcaaattcc ttggacaact atcaatcaca tcggggctaa
    50101 tgtttcaaga aaaaaagtta gccgccactt tgaaattttt acggaccaag gcaagttttt
    50161 atttgcttct ggagactctg gaaaaatctt aaaaatcact cgtcaacata ttggtaatga
    50221 gaaagtgatt acattaccaa ccttgatgca aacctttatt aacaaatttt ttcgacatta
    50281 aaaaaataat gcgcagtgcc gatttatcat ttcaagaaag caaagaatag tagctctaaa
    50341 ttgctattta gtggaaaaga ttccctcatt tgagtttact ttttcttgtt ttttgttata
    50401 atagtcctaa cggataagta ataccttttt ttcattcaga gagtctgtgg atgctgtgaa
    50461 cagataggga aaatgatatg aaattctacc gtgtcatcaa taaaatagag attaagtgca
    50521 cagattgtga agctggatgg aaccgcgcca aagcgctcca gcatcttcat aagctgtgct
    50581 ttttctatcg tcagagaatc acaagatcca actgtcttac ttagccttgt ttcactgacg
    50641 gctactaata actatcttaa aggagcttct gtatgctaga tcttaaacgt atccgtaccg
    50701 attttgacac tgtcgctgcc aaattaaaaa accgcggtgt ttctgaagac accctgaccc
    50761 atcttaaaga acttgatgaa aaacgtcgtg ctctattagt gcaatcagaa gaattaaaag
    50821 cagagcgtaa tattgcttct gctgccatcg ctcaagccaa acgtcaaaaa gaagatgcca
    50881 ctcaacaaat tgctgatatg caaaaagtct ctgcagatat caaaactatt gacaatcaac
    50941 tagtagctat cgaccagcaa gttactgaca tcatcactgt tttacctaat actcctcacg
    51001 attctgtccc tgttggagct gacgaagaag acaatgtcga aattcgccgt tggggaacgc
    51061 ctcgtgactt tgacttcgaa gttaaggctc attgggatct aggcgaagat cttgatattt
    51121 tagactggga acgtggagca aaggtaactg gtgctcgttt cttattctat aaaaatcttg
    51181 gtgctcgttt ggagcgtgct ctttataact tcatgctgga tgaacatatc aaagaaggtt
    51241 atcaagaaat catcacacct tacatggtta accacgattc catgtttggt acaggacaat
    51301 atccaaaatt caaagaagac acctttgaat tagcagatac caactttgtc ctgattccaa
    51361 ctgctgaagt gcctttgacc aattattacc gtggggaaat cctagacggc aaagaattgc
    51421 caatttactt tactgcaatg agcccatctt ttcgttctga agcgggttct gcgggtcgtg
    51481 acacacgtgg ccttatccgc ctacaccaat tccacaaagt tgaaatggtt aaatttgcta
    51541 aaccagaaga atcttaccaa gaactagaaa aaatgactgc taatgctgaa aatattcttc
    51601 aaaaacttgg tttgccatat cgcgtcattt ccctttgtac tggagacatg ggcttctcag
    51661 ctgctaaaac ctatgacctt gaagtctgga ttcctgcaca aaacacctac cgtgaaattt
    51721 catcatgttc aaatacagaa gatttccaag cacgtcgtgc tcaaatccgt taccgcgatg
    51781 aggctgacgg taaggtcaaa ctccttcaca cccttaatgg gtcaggactt gctgtcggtc
    51841 gtaccgtagc agccatttta gaaaactacc aaaatgaaga tggttctgtt accatccctg
    51901 aagtactccg cccttacatg ggtggcgaaa ccgtcatttc acctaaataa taaactcacc
    51961 ccagagatca ttaaagtgat ttctggggtt tactatttta aagagccacc acatcacttg
    52021 aaactcacta cattgcccct ttaacaacac ttttcttcac actagtttat caaaagtctt
    52081 attagtatac ccatttcctt atttacaaga ctagttaaaa acccttttgg aaaggcctcc
    52141 cttagcttga taaacacgta gggagactcc aaaaggatgt tgttaatatt tacgaaagcg
    52201 ttggtagcgc tcatctaata attggtctaa tggcttagct tgcaaactgg ttatttgttc
    52261 gatgaggtta gctttgatga tgtcaacgat ttcacttgaa aaataaccat gttctgggat
    52321 aatacgatct actatgccca tttgatacaa ttcgcctgcg gtaattttca tcaattcagc
    52381 agcctctgtc gctcttgaac catctttcca taaaatagaa gcaaaacctt ctggactaag
    52441 gactgcatac atggtatttt caagcatcca gacctgatcg gcaaccgcta aggctaatgc
    52501 accaccagag cctccttcac caataatgat agcgataatg ggaaccttga gatcactcat
    52561 ttccatcaaa tttttagcaa tggcctcacc ctgtcctcgt tcttccgcac cgacacctgg
    52621 ataggctcct gcagtattga taaaggtgac aacgggacgt ccaaattttt ctgcctgttt
    52681 cataaggcgc aaagctttac gataaccttc tggattgggc tggccaaaat tccttgccaa
    52741 attatcctgt aaattcttac ctttttgaat accaatgacc gtaacaggtt gtcccgccaa
    52801 ataagctaaa ccacctacaa tggcaccatc atctgaaaaa tggcgatcgc catgcagttc
    52861 cataaagtca tcgaaaataa ggttggcgta atccaaagtt gttaaacgcc cttgatcacg
    52921 cgcttctttt aaaactcttg atacatctgt cattggccac ctccatgaaa tgctactaag
    52981 tgggcaatct tgtctcgcaa ctctgttcgt ttcacaatag catccacaaa accatggtct
    53041 tgtaaaaatt ctgctttttg gaaatcatct ggcaagtttt cacgaactgt ggtctcaatg
    53101 acacgacgac ctgcaaagcc aaccaaggat tgaggttctg ccaaaataat atccccttcc
    53161 atggcaaaac tagctgttac cccaccagta gttggatctg tcaaaatagt gaggtaaaag
    53221 aggcctgcat tagaatgtct tttcacagct gcagagacct tggccatttg catgaggctc
    53281 atgataccct cttgcatacg ggcgcctcct gaagccgtaa aaatcaccac cggcagattt
    53341 tcttcaatag ccaattcaaa caatcgggta attttttccc caacaacagt tcccatacta
    53401 gccataataa agtgagaatc cataatagca agagcaattt gttgtccttt aaccatagct
    53461 ttccctgtca aaacagcttc atgcagacct gtcgtttcct tggctttttg taatttttcc
    53521 tgataacctg gaaaacgtaa cggatccttg gtttcaatac ttgtaaataa ttcctggaaa
    53581 gagccttcat caactgttaa ggtcagccgc tcttgggcag aaatcctaaa attgtaagaa
    53641 caggttgggc aaatctttgc caatcccaaa tctttcttat aaatcatgtg cttacaagct
    53701 gggcatttcg caaagagttc atctggaact tctgggacat tatggctgac agatcctttg
    53761 aggaaattat tgggagtaat acggatgtat ttatcttttt tacgaaataa agccatattt
    53821 tctcctaatc tttattgtaa tgtggcagga acgtttccat caagaaagag gtatcataat
    53881 ctcctgcaat cactcgctta tctgaaatca aatcgagctg gaagtctgta ttggtgataa
    53941 tgccttcaat ttccaattca actaaggcac gttgcatttt catcaaagcg tcaaatcgat
    54001 tgcttccatg aacgatgatt ttagcaatca tgctgtcata ataaggagga atggcatagc
    54061 cattgtaaac tgctgaatca actcgtagtc ctacaccacc acttggcata tacaagtctg
    54121 taatctttcc tggactcggt gcaaagttaa aagcagtatt ttcagcattg atacgacatt
    54181 caatagaatg tcccgtgata gtgatgtctt cttgattaat cgccaaaggc tgacctgctg
    54241 caattttgat ttgctctttg acaatatcca ctcctgtcac aaattcggtg acaggatgct
    54301 caacttggat acgggtattc atttccatga aatagaattt ctcgctgtct tcgtccaaca
    54361 aaaactcaat ggttcccgca ttttcatagg ctacagcttc agctgctcta accgctgctt
    54421 gccccatttc gtgtctgagg gtattgccaa tagcaatgga tggactttct tccaagactt
    54481 tttgattatt ccgttgcaag gagcaatccc gttctcccaa gtgaatgatg ttaccgtaag
    54541 cgtctcctaa aatttgcacc tcaatatgtc tagctggata aattactttt tccaaataca
    54601 tggcgccatt tccgaaagct cccaaagctt cttgactggc agcattgaaa gccgcctcca
    54661 aatctgcttc tgtctccact ttacgaatcc ctttacctcc accacctgcc gatgctttta
    54721 gcatgacagg ataaccaatc ttattggcaa tagctaaggc ttcttgagcg ttataaactt
    54781 caccatctga ccctggaata acgggcactc ctgccttaat catttcagaa cgagcattga
    54841 ttttatcacc cattttatcc ataaccgatg cggatggacc gataaattta atattcatct
    54901 cttcacacat ggtcgcaaac tttgagtttt cacttaaaaa gccaaaccct gggtgaatcg
    54961 cttgcgctcc tgtcacaata gctgctgaca agacggaatt catattaaga taggattcct
    55021 tggaacgggc aggaccaata cagatagcct cgtccgccaa tatcgtgtgc agagcttcct
    55081 tatccgcttc cgaataaact gcaactgtgg aaatccctaa ttctcgtgca gcacgaatga
    55141 tacgcactgc aatttcacca cgattagcaa ttaagatttt tttaaacata agggtttctt
    55201 cctccgcaga acaatcattc tcattgccca caggcaaagg ttaatgttcc acttgctgct
    55261 agcttgccat caacttctgc cctggcttca acgacagcaa tggtaccgcg acgcttgata
    55321 aaggttgctg tcatcacaag ttgatcacca gggacgactt gttttttgaa cttcacttta
    55381 tccattccag cgtaaaagac caacttgcct ttattttctt cttttgacaa ttctaagaca
    55441 ccagctgttt gagccaaagc ttccataatc agaacacctg gcatcacagg atagtgagga
    55501 aaatgcccgt taaagaaagg ttcattaata gtcacatttt taatggcaac gatatggtcg
    55561 tctgacactt ctaatacacg atcaaccaat aacattgggt agcgatgagg caaagccgct
    55621 tgaatttctc taatatccat catttgatcc gcaccaatcc ttgtccaaat tcaatgacat
    55681 cttcatttga taccaaaatt tctgtaatca caccatcaca tggcgctggc acttcgttca
    55741 tcaccttcat ggcttcgata atcacaaggg tttgtccttt ttttacagta tctccgactg
    55801 ccacaaatgg tggcttgtca gggctagctg caagataggc tacaccgact aaaggacttt
    55861 caacaatatc accttctgct acaaaagatt ctgcttgtgg ttgctcctct acaggaacat
    55921 ccttaactga tgctggacta gacgcttccg aagcagttgg attcggaaca agttgtacct
    55981 gtggtacagg cacagcatgt tcttgatttg atgtcgatgc attgaggtgt tgttcgtttt
    56041 tactaaagat tagctcacct tcatttgttt tgaataaaaa ttcacgtagg cttgaggtat
    56101 caaactgagc catcaaatct ttgatttctt gaatattcaa cctactcctc ccaacatttg
    56161 aaagctaata cagcattatg accaccaaat ccaaaagtat ttgaaatggc atagttgata
    56221 gctgtttctt ttccttctcc aaagatgaca tttgcttcga tatcctctga taaagcttgt
    56281 gtcccagccg tcatcggaac atagttatga cgcatagcct cgatagtggc aatagcttca
    56341 atagcgcctg ctgctccaag aaggtgaccc gtaaacgatt ttgttgaaga caccggaaca
    56401 tccttaccaa gaacggctac aattgcttga ctttctcctt tttcattagc tggggttgaa
    56461 gttccgtgcg cattgacata attaatagca ctggcttcaa taccagcttc ttgtaaggct
    56521 aagtggatgg ctttacgagc tcctaatccc tcaggatttg gagatgtcat atggtaggca
    56581 tcgcaggtat ttccataacc cacaatttct gctaagattg tcgccccgcg ttcttgcgca
    56641 tgttctaggc tttctaaaac aagcattcct gatccttctc ccatgataaa accattgcgg
    56701 tctttatcaa atggaataga gcttctgctt ggatcttcag ttgttgagag agctgttaaa
    56761 ctttggaagc cagcaatggc aaacttggtt atggccgcct ctgacccacc aactatcatc
    56821 acatcttgag tcccaaattt aatggcacga aaggcatccc caatcgcatc atttgaagaa
    56881 gcgcaggcag tattaattga tttacatact ccttgagctt ttaaagacat ggcgacattt
    56941 cctgcagcca tatttggtag cgcttttggc aacgtcattg gcttcacacg ttttggccct
    57001 ttttcatgta agcgaatcac ttgctcttca atctccgcaa ttccaccaat acctgaagca
    57061 acaataacgc caaatcgatc ggaatcaatc atctcaatat ttaaatcagc atgattaata
    57121 gcctctttcg ctgcgtaaag agcataaaga gaatatctat cgaaacggtt caaatctttt
    57181 ttcacaaagt atttgtcaaa tggaaaatcc tgaatttcag cagcattctt gactgcataa
    57241 tcagtcgtgt caaatttagt aatagggcca ataccaattt ggccagcttt cagattgttc
    57301 caaaacgttt caggatcatg cccgattgga gatgttagac cataacctgt taccactact
    57361 cttttaaatg tcatttcact acgtctcctt attgcattgt cataccgcca tcaatagcga
    57421 taacttgtcc cgtaatataa tcttgttcta ccaagaagct tgctaagtga gcaacttctt
    57481 gagcttttcc aatacgtttc attgggattt gacttaagat ttgctcttgc attttttcag
    57541 gaaggacacc tgtcatgtcg gactcgataa atcctggagc aatagcattc acgcaaatgt
    57601 tacgagcagc aacttcacgg gcaacagact tggtaaaacc aatcattcct gccttagaag
    57661 cggcatagtt tgcttgccca atattgcctg ttaaaccaac cacactagag acattaataa
    57721 ttgctccttg gcgggccttg atcattggtt ttaacactga ttgagtcatg ttaaaagcac
    57781 cggtcaaatt gattttcaag acgcgctcaa aatcctcttc cgtcatttta agcatcaatt
    57841 tatcattagt aatgccagca ttgttaacta aaacatcaat cgaacctaaa ctttcgatag
    57901 cttcattgac catacgttta gcttctgatg cttctgaaac atccccagag atggtaacaa
    57961 ctgtaacacc gtagtcagta aaactagcaa caagctcctc agagatagca gagcgaccat
    58021 ttaagacaat attagctcct aaactggcga attgatgagc cattgctaaa ccgatacctc
    58081 tcgttgagcc tgtgataaat atattttttc ctttaatttc catgaatgtc ctctctatct
    58141 aaaaataaac gtaagcttac catgttttca acactagtac atagtaaatt tttatcaatt
    58201 ttcttcacaa aacctgtcaa aaccttacct ggtcctacct caatgaattg tgttatgcca
    58261 ctttctacta aagtcgcaac actgtcataa aaacgaacag gctccatgac ttgacgggct
    58321 aatagttctg ggatacggtc tttttccata atattagctt cggtattacc aaccaaagga
    58381 atcttgaagt cactgaagtt gtatctttcc aactctttag ccaacaagcg gctagctggt
    58441 tctaacaaag cagtgtggaa aggacctgac acgtttaaag ggattaaacg cttaactccc
    58501 ctttccttta aaagttcaac tgccacgttc acagcatctg tctgaccacc aattacaatt
    58561 tgactaggag tattatagtt tgctggagca accactccat gtttagcagc tatttgacag
    58621 acttcttcga tgacttgcac gtctgtattc ataacggcaa ccatctttcc agatccttgt
    58681 ggtgctgctt cctccatcaa gcgccctctc ttagctacta aggatagggt atcttcaaaa
    58741 gagagtgccc ctgatgctac caaagctgag tactctccta aggaaagacc agctaccata
    58801 tccggtttaa cgtgatgtaa gcccaaaaca cggtaaatag caattgatga tgtcaaaata
    58861 gctggttgcg tatagctagt ttgattaagt tttaactcgt cactatcaat caaacggcgc
    58921 aaatcgtatc ccaatacttg actagcttga tcaaaggttt ttcttacaat agcaaagtta
    58981 tcataaaaat cccttgccat ccctaatttt tgggcacctt gaccggcaaa taaaaaggct
    59041 gtctttgtca ttatcattat ccttcatgtg tttatctact tttctattga aacagattgc
    59101 cagcgctgag cttcattttg aataacacga gctgcaccat aataaatatc ttttaaaatc
    59161 gtttcacagc tttcttcttt tctcacaagc cctgcaattt ggccagccat aacagatcca
    59221 ttgactacat cgccttcaat aacagcatgt cgaagcgatc ctgctcccat ttcttcaata
    59281 tcagtagctg ttttttgacc aattaaaaat gctttttctg ctttagcgta agctgaggtc
    59341 aatttatttt taatagaacg gacagggtgg cccacaacct gcgcagaaat caccgtatca
    59401 atatcttttg ctgctaagat tttatcttta aaattttggt gggcattgga ttctttagca
    59461 acaacaaagc gagttccaat ttgaacagcc tctgctccta acataaatgc tgctgctgca
    59521 ccatgaccat cagctatacc acctgccgca atgacaggaa tcgaaaccgc ttcaacaact
    59581 tgtcttacta aagacatagt cgttaacttg ccaatatgtc ctccagcttc cataccctca
    59641 gcaatcactg catctacccc aagcttttcc atacgtttgg ctagcgcaac gcttgggaca
    59701 acaggaacaa cgattatacc cgcctggtgc agtctttcca tatactttcc tggatttcct
    59761 gcgcctgttg ttactacttt aacaccttct tcaatgacca gatcaacgat atcatcagca
    59821 aaaggagata aaagcatgat attaacccca aaaggtctat cagtaatagc tttaacacgg
    59881 tcaatattgg ctttaacaac ttcttttgga gcatttcccc caccgatgat acctagccca
    59941 ccagcattgg acactgctcc tgctaaatca ccatccgcga cccaggccat acctccttga
    60001 aaaatgggat aatcaatatt aagtaattct gtaatacgtg ttttcataat aactttctat
    60061 aaaaaataag gagaataatt tgataatcaa actattatag taagaagaaa gagcgagcta
    60121 gatcctaaaa agagtctaat cccaccctaa tggtttgatt aatacttatt gtttttttag
    60181 tgatgaacat gaacataaca gcagtaagct tttcatagac ttctgatgtt acttgtcact
    60241 tttatcctta ttttgacttt tcctcaacgt aagcaacaag gtcaccaaca gtatttaatc
    60301 cttcttctgt ttcaatttga atatcaaaag catcttcaat ttctgaaata acttggaaaa
    60361 catcaagtga atctgcatct aaatcatcaa atgtagtttc tagtgttact tcttctgtct
    60421 ccttaccaag ttcttcaaca ataatttctt gtactttttc aaatactgcc atgtttattt
    60481 ctccttaaaa aatataaatt tgtttttata aatgtgctaa gcacatgaat aactagattt
    60541 taacaattag acttccccat gtcaaacctc caccaaaacc ggaaagtaag attttttggg
    60601 taccatctaa cctaatctgt cccttttgaa cagcctctga caagaggatt gggatactag
    60661 cagcactagt attaccataa cgcatcatgt tttcaaggaa tttttcccga ggaacatcaa
    60721 tcttgcgtgc tattttatca agtatacgac gattagcttg atgcaataaa caatagtcta
    60781 tatcatcttt tgtaatatct gattgcgcca ttagtgtgag gatgcttttt gacacatccc
    60841 taatcgcaaa atcaaatatg gcacgtccat ccatctgaat agtcgctatg gcttttttgc
    60901 caattgagta aggagatgaa agactagttt ctcctgaaat taaactctga catctcgcac
    60961 catctgtgtg taatgtttcc gctaaaacat gtttatcttt actggcttcc agtagcacgc
    61021 caccagctcc gtctccaaaa agaacggctg tcgctcgatc ctgccaattt actaacttag
    61081 ataaagtctc tgcacctatt accattccgt tttggtaggc acctgatgca ataagcttat
    61141 ctgccatagc caaggcaaag acaaagccac tacaagcagc agtcatatca aaagcaaaag
    61201 cacttgtagc cgcaatagct gcttgaactt tcgcagctgt tgatggcata gtggcatcag
    61261 ggctgatagt tgcaacaata ataaaatcaa tagcatcagc ttttaaccca gactgagtca
    61321 aaagctgatc agcaacctga atagctaggt cactagtcat ttcatctcta gaaatatggc
    61381 gttctgctat tcctgttcga gaaaagatcc actcatgact agtgtccatt atgctagcta
    61441 aatcattatt agtcactaac tgctgtggaa catagtgggc tacctgactg attttagaaa
    61501 aaatcattta aaccaaatcc tccaaaaatt gatgtagatt ccctagtcca cgcaccaatg
    61561 cctgcatctc ttcatcactc atatcagcaa tgacatgtcc aaccatattt ttatggaact
    61621 ttgcatggag gcggtccaaa agtcgacccc tttttgttaa tgacaggtaa acaactcgcc
    61681 tatctgagcg tgatcgcgtt cttgcgatat aaccttttgc ttctaattta tttaagctag
    61741 tcgttactgt ccctaacgtt accattaatt ctctggcgat atcacttgga gtcacctgat
    61801 catatttgcc gataatttca atagtgtgca tctcttttaa agagacatca ctaaactgac
    61861 tcgtctttaa gctcatttct tcaataacca aaatccggtt gaaaatatca actaagtatt
    61921 ggttaatttt gtcatattcc aagtaaacac ctcctagcaa taaactttga caatcaaatt
    61981 atatcaaaga tcaaagtatt ttgcaagcaa tagctgtctt acttaccttg aaaatttggt
    62041 cggcgccttt ctccaaaagc tatgacacct tctttaaaat cttccttgaa agctagctcc
    62101 tcttgaatag caagctctgc cttagcataa tcttcccatc ctgtgaaaaa gctttgccag
    62161 accaatgatt tcattccagc ataagaattt gaagagccac gtctcaatcg ctttaaaagt
    62221 tgaagacaaa ctttgtctag tttatcagat tctgccgtac ggtagacaaa cccataatcg
    62281 agacctttat cagcagtaat tccttcacct gtcatcacaa ggtgagttgc acgattaagt
    62341 cctactgcac gagtcaatag aaataaacca ccggcatccg gtgctagtcc aacatttaca
    62401 aaagcttgga taaattttgt ttgagtacta gcgatacaaa aatcaacagc taaagctata
    62461 ttaaaagccg ctccagctac agctccatcg gcgcaaagga tgactggttt tggcaaatgc
    62521 ttgattgcaa aagaaatttc ttgtaccagt tcagcaatct tgactagcga ctgcacatta
    62581 tcttttgcaa cagcttcttg catttcaact aggtcaccac caactgaaaa cacttttcca
    62641 acggctttaa ttaataaaaa acgcacagat gtatcgcgtt taacttctgc taatgctacc
    62701 agaatctctt gacaaattgg aatattaaaa ccgtttgaaa cctctggacg gtttaaagtc
    62761 aatgtcgcaa gatcatctac aacatcaaag atgatgtgtt taaactgcat aacaatcctc
    62821 ttttctgata atttatttga ctaagaaatt atttctcttt aaaatcattt gataatcaaa
    62881 tcaattacct aattatgata tactaaataa accattttga caagagaaaa aaacgatatc
    62941 tataaaagaa aacgttttct ctaactgttt tgacgtaaaa aaacgtataa cttcgggaaa
    63001 attatcctga aattatacgt ttgacatctt taaatatcct ctaaggcatc tttcacttta
    63061 tcaaaaaaac cttttttctt tggatgtaac attttttcac cactcgcttc tgcaaatgct
    63121 tgcaaggctt ctctttgagc gtcattgagt ttggttgggg tcacaatatt aacagtaaca
    63181 tgttgatccc cttgacctcc accacggagt tttggtgctc ctttgccttt gaggcggaag
    63241 gttttacctg tttgtgtccc agcagggata gccatctcaa catcgccatg aaccgttgga
    63301 atttcaacag tatctcctaa ggctgcttga gtaaaagaaa tatctaagtt gtagtaaata
    63361 gtggaaccat tgcgttcaaa ttgcttactt ggtaagacat tcaaaataac aaataaatca
    63421 ccataaggtc cgccattaaa gccagcttct ccttgacctt gtaaacggat ttgctgacca
    63481 gtctctacac cagctggaat cttaacagac actttatgag cttgcttttc atgaccagta
    63541 ccgtgacagg tttgacaggg ttctttaatt tccttaccac taccatgaca aatatcgcag
    63601 gtgacttgtc gacgcatcat accaagtggt gtttgggtgt caattgtcat tacacctgaa
    63661 ccatggcact tacgacaagt tactggagcg gtaccaggtt ttgcacctga gcctaaacaa
    63721 gtgccgcagg ttgcttcgcg attatagcta acttcttttt caacaccaaa tacagcctct
    63781 tcaaaagata aattaacacg atattgcaaa tcgtcacctt gacgaggggc atttgggttt
    63841 cggcttccac cgccaccaaa gaagcttgag aagatatctt caaaaccacc aaagccacct
    63901 ccgtcaaaac caccaaagcc gcccgctccg ccaccaaagc cgccttgagc acctgcagcg
    63961 ccgtattgat cataagcagc acgcttttgt gaatcactta aggtttcgta agcttcttga
    64021 acatccttat acttttgctc cgctccagct tctttattga tatctggatg atatttctta
    64081 gacatttttc gataagcttt tttgatatcg tcctgagaag cgtccttaga aacccccaga
    64141 cgatcgtaat attctgtatt gttcataatg taagatacca agccgtaaaa cgactcaatg
    64201 aaaaatagga aatccgacaa agatactctc gtatctagga ggtatttctc ttttttcgca
    64261 gagtcgtagg cgtgttcgaa tccgcaaaag agtctttaca cgctttagcc tgtgttcgaa
    64321 taatcgaact tgtattcctt tcttaaaata ttttgacgtc agcattttta cattttattt
    64381 agccaaaaac agaggtttgg tacaacctct gaattcggat attaatgtta ctagataact
    64441 aaatcattac ttttctgtaa attcgccatc tacaacatca tcattatttg ctgaatcatt
    64501 agcttgtgca ccttctgcac cttgtgctgc ttgtgctgct tgttgagctg ctgcagcttg
    64561 ctcgtacatt ttaacagcca aagcttgcgc tttttcattt aatgcttcaa gtttagcttt
    64621 catgtcgtca aggttgccag attcttgcgc agcttttaac tcgtcaagag ctgattgcgc
    64681 tgcatcgcgt tctgtatcaa agcctttacc ttcagtttct ttgattgttt tttcagtagc
    64741 aaagatagct tggtcaactt cgtttttaag gtcaacttct tctttacgtt tcgcatcggc
    64801 ttcggcatta gcttcagcgt ctttcatcat gcgatcaatt tcttcttcag aaagtccgtc
    64861 gtttgatttg ataacgatgt gttgttcctt ttgcgtacca aggtctttag cttttacaga
    64921 aacaataccg tttttatcga tatcaaatgt tacttcaatt tgtgggattc cacgaggtgc
    64981 agccgggata tcagtcaatt ggaagcgacc aagagtcttg ttatctgctg ccattgggcg
    65041 ttcaccttga agaacatgga tatcaacagc tggttggttg tctgctgctg ttgagaagac
    65101 ttgtgattta gatgttggga tagtggtgtt gcggtcgatc aatttagtga agacaccacc
    65161 cattgtttca ataccaagtg acaatggtgt tacgtcaaga aggacaacgt ctttcacatc
    65221 cccagtgata acaccacctt ggatagcagc acccatagca accacttcat cagggtttac
    65281 agatttattt ggttctttac cagtttcagc ttttacagct tcgacaactg ctgggatacg
    65341 agttgatcca ccaacaagga taacttcatc aatttctgac aatgacaatc ctgcatctga
    65401 aagggcttga cgaactggag ttttcgtacg ttcaacaagg tcacgagtga gatcgtcaaa
    65461 tttagcacga gataagctca tctccaagtg aagaggacca gcagaaccag cagtgataaa
    65521 cggtaagcta atttgtgttt gtgtcacacc tgaaagatct tttttagctt tttcagctgc
    65581 atctttcaag cgttgaagtg ccatcttatc ttgtgataag tcaataccat tttctttctt
    65641 aaattcagcc actaagaaat caataatttt ttggtcaaag tcgtcaccac caagtttgtt
    65701 atcacctgct gttgcaagaa cgtcgaagac accatcacct aattcaagga ttgatacgtc
    65761 aaatgtacca ccaccaaggt caaaaactaa gattttttca tccttgtcag tcttgtccat
    65821 accataagca agtgcggctg ctgttggttc gttaacgata cgttctactt caagacctgc
    65881 aattttacca gcgtctttag ttgcctgacg ttgtgcatcg ttgaagtaag ctggaacagt
    65941 aataactgct ttttctactt tttctccaag atagtcttca gcataacctt taaggtattg
    66001 aagaatcatt gctgaaattt cttgaggagt atattcttta ccatttgcag aaactttttc
    66061 agaagttccc atctttgatt tgatagagat tattgtttct gggtttgtca ctgcttgacg
    66121 ttttgcagca tcacccacga taatttcacc atttttgaat gatactactg aaggagttgt
    66181 acgattgcct tctgggttag caatgatttt tgattcagtc ccttcaagaa ctgctactgc
    66241 tgagtttgtt gtacctaagt caataccaat aattttagac atgttaatac ctcttatttt
    66301 ataatacttt attacttttt atttcaattt atcttagtaa gcactcgaat tattttactt
    66361 ccaattctaa gcaatcacta tagtttaatc agcaagcgaa atagcttcta acttgctatc
    66421 tagtttactg tcattacgga caaaaacgaa aactcattta ccttagttat aaacaactac
    66481 catcgctggt cttagcagac gctcgtgcaa tttatatccc ttttgaaaga cttctgcaat
    66541 gctatcggct ggatgttcat catcggctgg gagggtctga actgccatat gaaagttgtg
    66601 gtcaaacgag tcgacttcta cttcttctac gccttcttcc tttagtgctt gaataaggct
    66661 atcacgagtc atttctaaac cttttttaac atcatctgtc aacccttcaa cagccaaggc
    66721 acgttctaga ttgtcaagcg atggtaagat tgcttttgcc agatcctgag aacgatagcg
    66781 ttgaagttgt tgacgctctt cactagagcg acgctgtata ttctgcattt ctgcatgagc
    66841 tcgcaagtat ttattttcaa actcatcagc acgttcgttt gcaagttcta actctgattt
    66901 ttcaggagtt tcttcaataa cttcctcaac tgtctcttca gtttcagtgg cttccacttc
    66961 ttcttgtaaa tcgtcttttt taatttcttc tgacactgag tgcctccttt taaataactt
    67021 attgaatact gccattatca gtgaacttca taatggttag aatttaggta tcgataataa
    67081 tcacctaatt tagcagccaa tacctttcca ataatgttta ccaaactaac agacctacga
    67141 taatccatat caataggacc aatcaaactc aggagtccaa acccacggta aggaatcaaa
    67201 aatttgtgag ttaaaactga cacatctgca agtgcggctt cttgactatc tgccacttgg
    67261 acagaagcca tctcaccttc tttcagactt tgcctcaaag aaattgctac ttgctgttca
    67321 ttatccaaaa actgatacgt tgatagatct gaatacgtta aagaatttac cttgccagca
    67381 acaaataccg tttccaaaaa cagctctgaa aaaacataat caaatagttg taaaacatta
    67441 tccgtagtca caaaatactt ttgaacgatc tgaggaattt ctgtcctcaa cttataatga
    67501 atatctatga cactattatc cagcaagcgc tcctctacga tagctttaaa tgctatcaag
    67561 tcacgtgtca aaaagtttct tggaatagca aactgcactg tcactggctt agattcatct
    67621 aaagtcatga ctgctagagc atcatggttg ctaagctgaa caacatcaaa ccctgttaaa
    67681 cgttgacgag ctggctccac atctaaaata acggatgtat agcccgtcat ctccgctaaa
    67741 atgtgactag ctttttgaag catgtcttct aatttgaatg cttcaaaatc aaaagctttt
    67801 atgacatgat aaatatcttg ctcatcaata ctatctagcc tgagggagtg ctctacaaaa
    67861 tacttaaaac cagctggact tggcatccgt ccactagaag tgtgcgcttt ttccaacaat
    67921 cccaacttct ctagttttgc catatcatta cgaatagtgg cgctactaga atcaatcgtt
    67981 ctctgtaaag ctttagaccc tactggttca tgagtttgtg taaatagctc aacgattaaa
    68041 ttcaaaatat cattttgacg ttgtgtaatc acaaaccaac cactcccttt cgtttagcac
    68101 tctatttagt agactgctaa tttataattc tattatactc tttatgggag acatgtcaag
    68161 aaaaaaactc aaaaaaatta gcactctttt ataaagagtg ctaatttcag tcatagtttg
    68221 ttaaatcgtt actttcaatg acctgaatta gtcgcctagc ataggtttta tcagtataat
    68281 gcaaatactt ttgtaaactt ttagctggtg ctttataccc tttttctgta gcaagattac
    68341 gatacaaacg cttatcgact tctttgccct gccgaagcat tcgtaaataa tctctaattg
    68401 cactagcatc atctttataa atagcatatc tcacgttttg gttggtttgt ttaggggact
    68461 ttagggtaat ggctgcttgt cctggagttg ctttcttgct aaaaagatta tgatacttag
    68521 acgctagtaa tgtttttcca tcatgggttt ccaaaagaat ttgagcaatc aaaatagatg
    68581 gtctaatgcc ataatatttt gccaatggtt taacttcttt acccattttt gtaataaatt
    68641 gtttttggct gtagttcacc cttacttctt tatctgcact agctatcctt tggctactca
    68701 gtaagggaca aacaatcagc aataaaaagc aagctagaag cgttaaaaaa tacggaaact
    68761 ttagtcgttt tctcatcgtt tctcctttaa agcagcaatg tactcttcca acgttaggtt
    68821 gtgctgagcc atataacgag cagacgcctt gcctacatag cggtaatgcc aatcctcata
    68881 atcaacccct gtactagtct tttttccttc tggaaagcgc aagacaaacc cataatcagg
    68941 tgcaattttt tgaactgctt tagataccga tgggtcactg gcatttaaag tatctacggt
    69001 gcttatgtca atagcaagac cagtgtgatg ttcactagcc cctggcggct gtaaataggt
    69061 ttgcaccaaa gcctccgcag attcttgtgt caatgttgga tcgttagcca tctctttttt
    69121 aatataagat tggtaaagct ctgcttggta agctacggag cgatagcctg aaataaggtg
    69181 ctcttgtggg tcaatagctt gtgctgctgc taaaaagtca ctagtggctt gctcaatgcg
    69241 tttatccaca gaaataccat taatgtcagc taattcagga ctcatttctt ttgtaatgtg
    69301 atcgcggttt actaaaacca actcccaatc ttcaacagat accttaggta aagctcgtaa
    69361 acggtccgtt tttttgatat ctttttgttg gatttctttt ttattcaact gagttggtgt
    69421 tactgactct tctggtttta taaagaggta aagccctcct aaaaaaacaa taacaattag
    69481 caattgcact aaatagttta acgaattata tttcatctga ccgttcttct attatttcac
    69541 gaccgcggta gcggtaactc atatctccaa tagtctttat cgtaaattgg ccatcatcaa
    69601 attcaacaac tgtaacgctc ccattatcaa tatactgttt gtctcgatct ggatcaatca
    69661 accacagaaa cgttccaatt gtcataccat ggctaaccac aagagcattt ccgccgccag
    69721 cattttgaat ggacaaggca atagcttcaa acccctccca aatgcgctta cgaagaactt
    69781 cccatggttc tgcccaatta gcggtatcta tttccacgat gctttctgcc agttcagaat
    69841 agggaacatc acgtaggtta tcacgatttt caaaggcttt tgttcttgga aggacaccta
    69901 gaaataattc tgaatcataa gtcccttcta gactcccaaa acaccactca cgaatacgat
    69961 tatctttagt ataaggcaaa aattcgtttt ctgattctct taaaataatt tctattgtct
    70021 gcatggttcg acctgaatca cttgaaaacg cagccttaaa tggtatagtt gcatctttta
    70081 aacctaagcc aagttctcga ataccttctt ctcctttttt tgtaagaggt gtgtcactcc
    70141 aaccctgagc gcgaccaatg gtattaaaca tcgtttttcc atgtctggca atatacaacc
    70201 ttgtttttgt catttcaacc tcctaaactg tcttattaat tattatagca tttaaagaaa
    70261 acacttgcaa aacacttcct gatggctatt tgagatcaaa tagaccactg acttattgtc
    70321 tattttatta aaaataatgc tattgctaca attcaaatca tcacataaat ttccaaacga
    70381 ttacatttgt gtgtttcgct caaatgtata taacaacaga cccacactac aactaaactg
    70441 acgttacaaa atgatgtcaa aacagtaaca aaatctaact atttgtcaag aaaacgatat
    70501 taaattttca cacaactatc ttataatgag gatgcaacca ataaaaagat aagacatttt
    70561 atgttgcaaa aaaggctgct tggaaacttc catgcagcct ttttttaata cctttttcca
    70621 aaaatagctg aaacaagtac aattacaatt gctgctccca aaatggacgg gaaaatagcc
    70681 atacctgcca actgaattcc ccaatcccca aaaagccttt gcccaacagc tgaccctatg
    70741 agacctgcga taatattagt aaaacaaccc atggactggc ttttttcagt cattttacct
    70801 gctatcatgc cgataaaggc acctacaatt aaagaccata tcatcattaa cacctccttt
    70861 cttgtaaaat ccttttagaa gaactgctat ccattaaatg ttcttaactt tagttgattc
    70921 ctttaccttc ttctttagta agtgtttccg tgcctgagta aaatagaaaa aacaattcct
    70981 tctattaaga tagatcgaga acgactagaa gtcttcgcac actaaataac ttaacaaact
    71041 ttatcacaca gctaatcgct aataatagtg tagacaaacg tgatggttca ttaaataccc
    71101 gtcagaaact cctgaaggtt ctaaagcaat acctgtcagt cttattttag catagatgat
    71161 ggtcttttta taggcggtca ctaacatgac atacaaaaag gcagcctgtg aaagaccgac
    71221 cgcctttttt tcagagacaa aatactttaa cttattgcta tcagcaacta tcaatctagc
    71281 aatttttgca attcttgtgc caataattgt tgagcgactt gtggattagc ttgacctttg
    71341 gttgccttca tcaagaagcc tgtgaaagcc ttatcagcat tacgtttgcc tgatttgaaa
    71401 tcagccactg cagcttcatt atcagcaaag acttggtgaa taattggtac caaaacagcg
    71461 ggatcagaaa tctgaacaag ccctgccttt tcgacgtagg cgcgagctga gccaccgttt
    71521 ttagccaaat gcacaaagac cttcttagcc attttagaag agattgtacc atcagcaatg
    71581 atagcaatca tctccaccaa attttctggt gtcagagcga tttcttcaat ggttttgcct
    71641 tctgcattta agaattgggc aacttcccct tgcaaccagt tagagacttg cttggcgtcg
    71701 ccaccaagac ttactgcagt ttcaaagaaa tctgacaagg ctttggtagc tgttagttgg
    71761 ctagcatcat aagttgagag accaagttcc tcttcgtatt tagcacgacg ttgagctggg
    71821 aattgaggta attgagcacg catctcatca atccaagcat catcaatttc atataagggt
    71881 aggtctggtt ctgggaaata acgatagtcc gctgcccctt ctttgacccg catcaaaata
    71941 gtccccttat tcgcttcgtc atagcggcgt gtttcttggc gtatcacacc acctgaacgc
    72001 aagagtttgg cttgacgttc cacctcaaat tcaagccctt tacggacatt tgagaaggag
    72061 ttcaagtttt tcaattcggt ctttgttcca aattgttctt gcccgtaagg acgaagagaa
    72121 atattggcat ccacacgcat ggagccttct tccatcttga catcagaaat acccgtatac
    72181 tggatgattt ctttcagagc tgtcaaataa gcataggctt cttcaggaga acgcatgtcg
    72241 gcttctgaca cgatttcaat caaaggaact ccttggcggt taaggtccac ataagaataa
    72301 ccatctgtgc catgagtgtt tttaccagca tcttcttcca agtgtgcgcg ttcaatacga
    72361 attttcttgg ttgagccgtc ttccaattta atctcaatcc atccgttgta gccaatcggc
    72421 tcgtcaaact gtgaaatttg ataagcttta ggattatctg ggtagaaata atttttgcgg
    72481 tcgaaatgca tctctttatg gatgtccata ttgagggcaa gcgcagcttt tatgcccgca
    72541 tcaataacgc ctttgttcat gactggtaag acccctggga aagaccaatc aatcacattc
    72601 gtgttggcat ttgggtcttc accaaagtga gctgacgaag gtgagaaaat tttggaattg
    72661 gtatttaatt ccacatggac ttcaagccca ataatggttt caaaattcat tacttgtcac
    72721 ctccaaaaat aatcggttgt tgcttgtgat aatctgtcac cgcttcaaag gctgctgctg
    72781 cctgatagat ggtttcttca gcgtatttcg gaccaattaa ttgaagccct actggcaagc
    72841 catcgacgaa tcctgccgga atagaaatac ctggcagacc agctaagtta accggaatgg
    72901 tcaataagtc ggccaaatac atggcaacag gatcatggtt gagtgtgtct aagccaaaag
    72961 caaccgtcgg tgttgttggg cctaaaataa gatcataatc cgcaaagacc ttgtcaaaat
    73021 cttgaataat caaagtgcgc acttgaccgg cctttttgaa ataagcgtca taataacccg
    73081 atgacaaact aaaggttcca agcatgatac gacgtttcac ctcatcacca aagccttggc
    73141 tacgagtgtt aacatagatt tcatccaagt ttttagcatc gtcagcacgg aaaccatagc
    73201 gaatgccatc aaaacgttgt aagtttgaag acgcttctga cgaagcaata atgtaataga
    73261 cagccacacc atatttgcta tgtggaagac taacttcctc aacagtagct cctaaggctt
    73321 caaattgttt agctgccgca agaaccgttt ccttaatctc aggatcaatc ccttcgccaa
    73381 gatactcttt aggaagggca atcttcatgc ctttaatgtc acgaccaatc ttagatgtgt
    73441 agtctgcaat gcggacaggc gctgaagtgg catccttgac atcgctactt gcaatcacat
    73501 ttaataattg ggcgttttct ttgactgtcg gagcaaaagg tccaatctga tcaagtgaac
    73561 ttccaaaagc aatgagacca tagcgagaaa cagtgccata ggttggtttc aaaccgacaa
    73621 caccattaaa tgcagctggc tgccgaatag aaccaccagt gtctgaacca agagataaac
    73681 ggacttgtcc tgaagcgact gctgtggccg aaccacctga tgagccacca ggtacttttg
    73741 tgtggtccca agcatttttc gttttcttga aataagaggt ttctgttgaa ccacccatgg
    73801 caaattcgtc catgttggtt tttccaataa caatcatgtc tttagcgtag gcatttgcta
    73861 ctgatgtggc attaaaaatc ggctcgtagt tatacaacat ttttgaagcg gctgtggtta
    73921 ggatttcctt ggttgaaata ttatccttaa cagctaacgg aatacctgac atgaggttat
    73981 cagcatcaat acctttggca tcaattgccg cagcttgttt aagagcaact tcttctgaaa
    74041 cagtgataaa cgatccgaca gcctcctcac gagacttgat gtcttcaagt gttgcttggg
    74101 tcaattctgt tgctgaaatc tccttagcta caaggaggtc atgcaactct tcaatggttt
    74161 tatgattaaa agacattagg catctcctcc gtcatccaaa atagcaggta ccttgataaa
    74221 gtgattttct ttttcaggaa catttttaaa gagcaaggca cggtctgttc cttcttcagc
    74281 gacatcttga cgcatcacat ttttcttatc tgccatggtt gttgtaatgg cgacaccttc
    74341 ggtatcaact tcgttcagca attcaaccat atcaacgatt ttggataagg tcgttgcaaa
    74401 ggttgttgtc tcactttctg aaaatgataa ttttgatagc ttcgcgacat ggcgaacttc
    74461 ttcttcagaa attttcatga tttctcccat ccaagatgat cagaagtatt ccaattccaa
    74521 agaacagccc tgctattctc caaaggtagc ggaaaaactc ctcacatcat ttttacatag
    74581 tctcaattat tatatcacaa ttgctcccat ttaccatagg tgactaatgc aattctagaa
    74641 agccatacag caaaaaactt cctgactaag gtcagaaaac cttagtcagg aagtgtgaca
    74701 aactaaactc tatttgtctt gggtaagact gtggcctgaa gctttgatgg cagcctgagc
    74761 agccgccaag cgagtaagcg gcactctaaa tggcgaacaa gaaacgtagg tcaggccttg
    74821 gctgtggcaa aaggcaatgg aagcaggatc gccaccatgt tccccacaaa taccaagttt
    74881 caactcaggc ttaaccgaac gggctaaaga gacggccaat ccctttctga tcaaaggtct
    74941 ggaacggctc cttatcaaaa atacccctgt caacgtattc ttccaagtat tttcctacaa
    75001 acatcacaga aaaagtaacg ttctttcaag tcggtaatat gtttacgaag gtagtaaagg
    75061 ggtgctttat tctaatttac caaatcctca acgattgtaa caacatgtga tgtgataagc
    75121 ctaagataaa aatggtacag actctagcat tcaagagttt gaagctttat ttatattcta
    75181 atgctgaaaa gactagagtt tgtctaatgg ctgtgagacg atcctctaaa ccatcaactc
    75241 aaatcatttc tcaaacagct aaaatcctgt ctccttcaaa aaaacatgtg ttctatctga
    75301 cacagtccaa agtttagttg ccttttagac agcacaagaa tcatcaggac taattctgac
    75361 cagcatgtca catacctcat ctacctaacc catacatacg gtaaaaccac atagagcaat
    75421 gattacggtt accaaactct tagtcactga atcaatagtt tggtaggctc ttttagtaat
    75481 cgttaataag cttagcacca agaacctgct caaagtgagc caaagaccac tcataagatt
    75541 cttttgtaag gctagcgaca gccgatttaa caatctctaa ttgataacca agatgataag
    75601 cgtcaatagc agtatgtaag acacagatat cagataaaac tcctgttaac actaattgag
    75661 tgatacctct ttctcgtagt cgaatatcta aatcagtccc cgaaaaagct gaataataac
    75721 gcttatcaat ccaaaaaact ctgggatgtt gcttcatatg actgtaaact tcagctagtg
    75781 gaccataaag atggcgtcca gtagttcctt tgatattatg tgctgcaaat agcttactct
    75841 caggatgcca aggatcattt tgatcatgac aatcaatagc aaaaaaaata tagtctcctt
    75901 gatcaaatgc cgttttagtt acctctgcta ttttagtggc aatagcctga gcagatttac
    75961 ctgcacttaa tttaccatca tctgctacaa aatcatttgt gtaatcaatt gaaatcaatg
    76021 ctctcatcac tatctcctaa acagggaaac ctctcccctt atctcttata tatggcattg
    76081 tatcagtttt tattacaggt atcaagacat ctatttgcat ctaccattaa cagcaaaaaa
    76141 catctctttt caagagatgt tgtctttttc tatttaaaat tcttttaatt tagcaaaaat
    76201 accttcgttg ataactttga ggtatgtccc tttcatacca agagaacgac tttcaataat
    76261 ccctgcactt tctagtttac gcagagcatt gacaataaca gaacgggtaa taccaatacg
    76321 atcagcaata acagaagctg ttaatcgtcc ttcattacca tctaactctc caagaattgc
    76381 tgcaacagct ttcatttctg aataagaaag cgtattaatt gccatgttga ccgctgtttg
    76441 tttacggatg gtgtcttcta agttttctgt ctgaagattt aataattgaa tccccacaac
    76501 agtacttgag atctcaacca aaatcaaatc atcatcacta aactcattgt cattacgcca
    76561 gataatgagt gatccaaggc gcatccctcc accataaatc ggcgcaatag tcgttagacc
    76621 tcctggataa gtgtctttag actcaacagg gaatatagtc aattcatttt cgacagaaag
    76681 gttagcttct gtatcgtaaa ctcggctagc agcctttaca taagtatctg gaaattgttt
    76741 agcttcaaaa aattcctcaa cacgatcagt gttagtttta tatttcatgg cataaccaag
    76801 cagtgtgccg ccgccattga taatacaagc attgcagtca atgatatctg ctaggcgaga
    76861 tgccattgtg ttatacggta attctgtttc taggctatct acggaacgct gcaaaataga
    76921 tgtgatttta cgagtttttt ctaataagtt aggcattttt ttccttttcc tttgaattat
    76981 ttgcgctttc attataacaa agctaactcg cttttgcaag gaattatcag aaaattttct
    77041 attcttacta caattccaac tattgatgcc ttattatcac ttcatttaaa ggagataagc
    77101 actagcttgt tagagcatag ggtcacattc ttttaagaaa cgctattttt attaatcaaa
    77161 gatgagcatt gcattaacgt ctatactggt ataagacacg tgtaatcttt tcttggacat
    77221 cttctaattc ttctacactt ggtaggtaaa caatacggaa gtgatctgga tctttccagt
    77281 taaaaccacg tccatgtacc aacatcactt tttcttgctt taataactgt aagacaaatt
    77341 cttcatcatc atcaatacga tacatctgac gatcaatttt cggaaataaa taaagacctg
    77401 cttcaggttt aactgccgat aagccaggga tagcattaat agcttcataa ataaaattac
    77461 gttgctcaaa aatacgccct cctggcagta acagtgcatc aactgattga tggcctccta
    77521 aggaagtttg aacaacttgt tgggctaaaa cattggagca taaccgcata ttggcaagca
    77581 tgttcaaccc ttcaatgtaa cctttaacat ggtctttagg acctgataag accatccatc
    77641 caacacgaaa accagcaata cgatgagatt tggaaaggcc attcatagat acacaaaaaa
    77701 catcaggtgc caaacttgca atagcaatat gctctttacc atccatcacc aaacgatcat
    77761 agatttcatc ggcaaaaata atcagctggt gttctcttgc caaagcaacg atgtcttcta
    77821 aaatctcctt aggataaagg gctcctgttg ggttattagg attgatgacc acaatagctt
    77881 tggtgcgact cgtgattttt gatttgatat cagcaatatc aggataccaa ccagcttctt
    77941 catcacaaag ataatggacc gcttttcctc caccaagact cacacaagcc gtccataaag
    78001 gataatccgg cattggtact aaaacttcat cgccattatc tagcaaagct tgaagggaca
    78061 tggatatcaa ctctgacacg ccatttccca aataaatatc ttcaatgtca acatctggga
    78121 agcccttgag ttggcaatac tgcatgatag ccttacgcgc tgaaaaaatc cctttactgt
    78181 cagaatagcc ctcacttagc ctcgcgttga caattaaatc acgaatcact tcatctggtg
    78241 cttcaaaacc aaaagcagca ggattgccag tatttaaacg cagaattttt tcaccactcg
    78301 caatcatgcg attagcttca tccaaaacag gccctcgaat atcataggca acatgttcta
    78361 attttgatga tttttcaata attttcatag tctcatacac ctcgtattcc atattataaa
    78421 ccaaaatact taaaatagaa agaatttatc catcaaagct taggacagct aatcgtttta
    78481 acacaggtct tatgagacga taatacagtc tttagtttag caaaaataat ggatcgcttt
    78541 caaaatgact caaaaaagag tataatgaaa aaaagaataa aagctattct tgtcaaactg
    78601 ttaaaggagg acagttctat gtcactaaaa tataaacgta tcctcgttgc tattgatggc
    78661 tcttatgagt ctgaattagc tttcaataaa ggcgttaacg tagcactacg taacgacgct
    78721 accttactac tagtacacgt tattgatacc cgtgccttgc aaagcgtagc tacttttgat
    78781 acttacatct atgaaaaatt agaacaagaa gctaaggatg ttttagatga ttttgaaaaa
    78841 caagcacaga tagcaggaat tacaaacatc aaacaaatta ttgaatttgg caatcccaaa
    78901 aacttactag ctcacgatat tccagatcgt gagaacgctg atttaattat ggtaggagct
    78961 actggtctta atacctttga acgcctcctt attggctcat cttctgagta cattatgcga
    79021 cacgctaaga ttgacttact tgtagtccga gatagcacta aaacactcta gtctattaca
    79081 attttcaaac accaaaaaac aagcagcatc tacaacgaaa ttgcttgttt tttgtgctga
    79141 gctaaggcta gtcaatatct ttttatttct tgatatttaa aagagtaaac ctctatttat
    79201 cgaatcgttg tagtgatttt tgtaattgtt tgtctaactc tttttttatg gctacttcag
    79261 gtgcgtgacg ctcttgccaa tagtcaggtt tttgaatttt atgggtaacg gggtcaaagt
    79321 gtgccttgcc atctggaaaa attttagcca tatttgcttc atgtactgct tcaaatatag
    79381 gttgtggatc tactcccatc agtacaaaag aaccataagt aaaataaagc aaatccgcta
    79441 aggcatcaac ttgcccaatc aggggcgttt caacatgttt tttagcttgt accttattag
    79501 ctgcttggtc aacagcacca tgcaaatcga aaatagcctg tgtaaattgt tgttggtctc
    79561 ccttgcttgc tgcgtaaaga aactcaacga tttcttctac tttgaagcct gacctataac
    79621 cagcttctga aatggtataa ctacgcggtg tttcaatagt atctgagtcc atcagacgat
    79681 gaaaatcttt gactttatta aaattttcat cacggctttt aaagctcttc tctacatcaa
    79741 aatggatgag accgtagtga gctaaggctt tggaaatccc atcattatta ttagagtcgg
    79801 tagtgaaatg ggctccatct tttaccacag tttcagcatt tcccatagca acaccgatac
    79861 caactcctga taacatttca aggtcattat ctgaatctcc aaaagccatc acttcagaca
    79921 agtcaaatcc aaacatctct cccaaacgtt caattccttt tatttttgat tgatcaacag
    79981 agatcagatc caaggaataa ggactacttc ttgtaatttt tatatgaggg aacttctctt
    80041 gtattttctt agtttcagct tgtgacgcta ccagaacaac ctgataaata ggctcccgca
    80101 taatagttac taaatttcta aaactctgtg gcttgattcg gcgaatcaaa tgcttaaaac
    80161 taccctcaac tgttcttgcc caacttttgg gcacaaaact actaatgact tgtccaaatg
    80221 ggctagtccc catatcaata atacgcgatc ctgctaatcc agaagctgtt ccaagagaaa
    80281 tttctcgctt tttttcgttg gcatagcgaa tcactttgta aatcatcgac tttggcaatt
    80341 gattctgata taagacctta tctcttgtta agatatattg gccattgtaa gtaacggcaa
    80401 aatctaatcc aaaattttct agaaaaggtt gcacaaatcc cggtccacga ccagttgcta
    80461 aaccgaccat aatgccttgt tttttgagtt gttggatggc tttttgcgtt gttttttgga
    80521 tatttttacg atcattgaga agggtcccat caatatcaaa aaagactgct tttactgtca
    80581 aaaccttctt ttgcctcctg tgtttatgat agaatagatt ataccataaa taaaaggaag
    80641 tttgacaatc aaatgaaaaa aatcctagtc ttacacactg gtggaaccat ttctatgcaa
    80701 gctgataact ctggtagggt tgttccaaat caagataatc ctatgacgaa aatacatgct
    80761 gcagcccaag atattcagct taccgttagt gattttctaa atcttccgag tccccacatc
    80821 acgcctcacc acatgttatc tatttatcat catattcaag aaagaactga tgtctttgat
    80881 ggtatagtca tcacacatgg aacagatacc ttagaagaaa cagcttattt tttagatacc
    80941 atggcattgc ctactaatat acctgttgtc ctaacaggtg ctatgagatc ttctaatgaa
    81001 gttggtagtg atggcatata taattacctt accgcactta gagttgcaag tagtgataaa
    81061 gctaaagaaa aaggggtact tgttgttatg aacgatgaaa tccatgctgc taaatatgtt
    81121 actaaaacac atacaaccaa tatttcaact ttccaaacac ccacacacgg accgcttggt
    81181 atcatcatga aaaatgacct actttttttc aaaactgctg aacctcgtat tcgttttaac
    81241 ttgagatgca tttctggaac catccccatt atcaaagcat acgcaggtat gggcgatggt
    81301 agtatcttga gtctattaac tccaggtagc attcaaggtc tagttatcga agctctaggg
    81361 gcaggaaatg tcccaccatt agcagttggg gagatcgagc acttaattac tttaggtatt
    81421 cctgtcgttc ttgtttcccg ttgtttcaat ggtatggcag aacctgtcta tgcttatgaa
    81481 ggtggtggtg cgatgctaca agaagcaggt gttatgtttg taaaagaatt gaatgctcca
    81541 aaagcccgtc taaagttatt aattgctctc aacgccggac ttactggtca agagctaaaa
    81601 gattatattg aaggataatc tagcttcctg tagaaatgtc ccactaaaac atattagtgg
    81661 gacatttcta gtactttaag ctttaaatta ggacatttcc taaatgtcta taataaacaa
    81721 ctatgtttca aactatgaga ttgtgcactc ataatcattt ctataacagt ttgagaccag
    81781 tattccaata aatccaactc ttagcaatca gatactgggt aaacaagtct ttattttgtc
    81841 tcattaagta tgagcttact tgaataataa caaaactact tgctcatcct tttttagtaa
    81901 tgatgaagac gacctttttc taagaacttc tttgtctttt aaaataaaca aagcacatat
    81961 taccctttat tttagttctc gtttggaaaa aagaaagaca ttgcctaaaa aataacaaag
    82021 agttaaacca aaggccacta atatgctagt actagaaagg tgtttatcac caagagacaa
    82081 ggataggtgt ttaaacatca aaactgggct aatatctaac ttaaaaaaat ggtttgctaa
    82141 agtagcagat actttatcaa aaagaagaac ataaagtata taagctgtga ttgactgata
    82201 agttttacga agtatattaa acaaaaattg acacaaagca ataatagcaa tataaaaagg
    82261 cagttgttta atcatccgat ctatgactat tccatagtat gcttgaccag taaataaatg
    82321 aaatgctaaa gcactaagaa gataagttaa actaataaat aaaagagata agccaaaact
    82381 aactagcagt ttatagatta taattaaagt acgattttgt tgtttgacca caacattatt
    82441 aatcgtacgg tagttatagt catcactaaa aaagatacgg gctggtgtta aaaagaaaag
    82501 cgtcacaaaa tcagtaataa tattaatgat ttgtatcatg atgacttggt taggtgtctt
    82561 gcgaaaacta taggctacac caacagcaat gacaacaagt agcatacaag aaaatagagc
    82621 taaacgctct tggcgtatcc gaaataaatc tgctttaaga taggaaatca taaaaacctc
    82681 tttctaaatt aatggtgacg ctgcatcttt acctaacaaa tcgaggtaat aatgttcaaa
    82741 gctcacttga tgatgataaa tgtcaaaaat ctcaacaggc agatctagaa caattttgat
    82801 aagctccatt actgataaag ttggttttat tagtagcatc tgaccatcag tctcaactct
    82861 aagccccaat tctaaaagtc tattttttac cagctgatca tcgcttgtat gaagggcaat
    82921 ttgtactttt acttgagctt taagctctgc tttgtccata ctcttaataa ctttgccttt
    82981 atgcataatc acgtaacgat caacaactaa gtctaattct gataaaatat gactggaaat
    83041 caaaatcgta ataccatagc aatctcttag gttcaaaata atctgacgca tttctttgat
    83101 tcctgctgga tcaagaccat taataggttc atctaagatt aaaaattctg gaaaatccag
    83161 tatcgaaaga ccaatagcca aacgttggcg catccccaaa gaataatctt tcaccttttt
    83221 ctttttgctc actttttcta acccaataag ttccaaaacc tcatgtatgc gctcttttgc
    83281 atcagctaat ctcaattgca acgctgcata gtaaagatta tcatgagctg tcatattggg
    83341 ataaagtgtc ggtgactcaa taattgatcc cattcgatag ggttgtaact gtttgatttg
    83401 ccctgaatct tttccaatta ggccagataa aatacgcatt aacgttgtct ttccagcacc
    83461 attttgaccc acaaggccgc aaatttctcc tttgtaaata tcaaaagaga tatcacttaa
    83521 aacctgattt ttaccatagg atttatttac ttgctcaata cttagtaaca ttgattgctg
    83581 tgtcattttc cgctcctttt ttgtacttat cgattaatac aatatcttac acataataac
    83641 atctgtaaac ggttgtgaca agttcttctt tttattaaaa aaatataaat aagaatttta
    83701 ataatatgat tattttagca ataaaaaagt tatactgaaa atgggttaat actgcttcaa
    83761 atagcagtct catcgtaaag cataagtaaa aaaggtcaga aatagtgacc tctttatact
    83821 ttataacagt ttatctcaaa gctaactaaa tatttgtaaa acaacgactt gttttaagtt
    83881 aatcataaac ttcttttttt ctaatatttt ttgctactaa ttgccactgt ttttcatata
    83941 tccagttagg atcagaaaca atcgctgcag aaactttgcg tgcttcttct aaaataggat
    84001 aatcctcaac gatatcagct acttgaaatt ctggaattcc agactgacga gtaccaaaga
    84061 tttcaccaga accacgcatt tttaaatccg actcagccaa aacgaaacca tctgtcgttt
    84121 ctgtcatgat tgtcattcgt tttttccccg aatccgtttt gggattagcc actaaaacag
    84181 cgtatgattg tttatgtcca cgaccaacac gcccacgaag ttgatgtaac tgacttagtc
    84241 caaaacgatc ggcatccata ataatcatga tcgttgcatt tgggacattt acccctactt
    84301 caataactgt tgtggatact aaaatatgac tttttttatc cttgaaatct tgcattatgg
    84361 catctttttc atcatttttc atacgtccat gtacaagagc aaccttagca attccttcaa
    84421 aataagtaga taattcggca tgcaatgcta ctgcattctt taaatctaaa gcttctgatt
    84481 cttcaatcaa cggtgaaatg acatacactt gagcatcttt ttgcaattca ccttttaccc
    84541 attccaacac agtccctagc tgctcgtgtt tcacccagcg tgtcataata ggtttacgac
    84601 cggcaggtaa ttcatcaata acagaaacat ccatttctcc aaaagctgtg attgctagag
    84661 ttcggggaat tggggtggct gtcatcatta aaacatcagg attttctccc ttttcacgga
    84721 aaattctacg ctgtttaaca ccaaaacgat gttgctcgtc tgtaatgaca agccccagtt
    84781 tatggtactg taccgagtct tggataagag catgagttcc tacaatcata tccactgagc
    84841 catttgcaat agctgctaaa accgtacgct tgacagcggc tttcataccc gaagttaata
    84901 tagcaattga tagatctgga aataactctt gcagactaat gtagtgttgt tcagctagga
    84961 tttccgttgg aaccatcaaa gccgattgaa aacctgctgt ataagctgca tacattgata
    85021 gaccagcaat gactgtcttt cctgatccta catctccttg caataaacga ttcatatgag
    85081 ctcccgatga catatcagat aatatgtcat ctaaagagcg cttttgagca tttgttagaa
    85141 taaagggtaa agaggaaatc tttgtctcca tagcatgttt actataaaga ataggcaaac
    85201 catttgtttc agatttattt tcggctttca aaacttgaag gttcatttga aagtaaaata
    85261 attcttcaaa tttaatccga cggagcgctt gcttatactc tgtgatatct tttgggaaat
    85321 gcatagctaa acaagcctga ctacgaccca tcaatcggta tttttccaat aaagtagctg
    85381 gtaaattttc cttcaattcc aaatgcgcat cgatttcaaa agctgactta atagctttaa
    85441 tcaaagtaga ctgtgaaatt ccctgagcta cgcgataaac aggttgcatg tcatcttcaa
    85501 cttgagctaa aaccttcatc ccagtaatag ccgatttagt ggcatcccat ttaccaaaaa
    85561 cagctacctc ttgaccaagt tctattttat cagctaaata gggttgatta aaaaaactaa
    85621 catttaagac agcttcccct tgacgcaatt tgaaacttaa acggttacgt ttaaaaccat
    85681 aatattgtac attagctgga gtaacgacta agcctgtaat gactgctttt tcaccatcca
    85741 ctaaatcaaa aacagatttt gatttaaaat cttcatagcg aaacggataa taaagcagta
    85801 aatcttctac tgtataaata tctaatttct gaaatttttc tgctgatttt ggtccaaatc
    85861 cctttaagtt ggacatagga gctgttaata tcattatctt tttcccttca caaaaactct
    85921 tgtcagattg aagacaaagt ttgtcgtgat actttcctta ggtggtgctt acggcagcga
    85981 cgttctttgg aattccctgc ctttattttt gagcctgagg tctcaaaaat cccgagtggc
    86041 gtcaatctcg tattgacgcc actcttttac cacagcaaaa agcatctgtt ttggctcact
    86101 ttatatgtaa aaatcggaaa ccgaatgtca aatcagcaga gctatgcatg ctttttgaac
    86161 ataaaactgg ggttgtctac gttctgactc ttgtatctat tatacaagag ttcctattta
    86221 ttttcagata aaagcctttt agtactttta aacagttcta ggggatcgtt aaggtagcat
    86281 ttgataaatg gtatcgcaga cactacttag aagttcttca tcatgtgaaa tgaggatgac
    86341 aaccttatgt tgggtcttca gctgcatgag aagattagct aaagccttca tttgtaacaa
    86401 atcaagtccg ctacttggct catcaaaaat taagatatct ttatcagcca aaagactagc
    86461 ggcaatcatc actctttgtt gctcacctcc tgacaaacta gctggatgac gttctaaaag
    86521 agtcgttaaa gataaccgct ctattacttc tgtcgtccgt ggatgtcgtt catggcccaa
    86581 gttgacttcc tttgaaaccg attctgcaaa tagttgcaaa gaaacttctt gcaagacaat
    86641 actagtcttt gataaacgtt gcttggcaga taaaggtatg ccttgaaact taatagttgc
    86701 ttttttatcg tctaagatac cagctatata ataaactaat tgcgattttc caagcccgtt
    86761 tgatcctgta atgccagaaa tacttcctgc agcaaaacta agctgttcaa tacatctaag
    86821 tattttgctt cccgctctaa ctgttagatg ctcaatacac aggctatcat ctgggcgata
    86881 atgttgactc tcaatcttac cagctaacac aggttttagg tcagacaagt ctaagcgacg
    86941 tagtcccata tcttgccgtt gttcatctgt caaggctagc aaatcttgtg ttgttaactt
    87001 atctgttaat ctaccatttt taaagtagaa aaagttatct gccaaatcca tcaagtaatg
    87061 taaacgatgc tcggctacaa taatggtttt tcctgctgct tttaattggg taagataggc
    87121 tttaacagca gctatgcctg cactatctaa gttagcagtt ggctcatcaa aaagcataat
    87181 attagtacct tgcataatgg ccgttgcaat agcaactctt tgtttttgcc ctcctgacaa
    87241 tccaaacata tccttgttaa ggagctctgc aaaagcaaaa tcttctgcta aagtccaaag
    87301 acgcttcatg ataacctttg catctagacc ttgattttca cagggaaaga ccagttcgtg
    87361 ttggacttcg cgatagaaaa actgactggc tggattttga aaaacagagg ccacagatct
    87421 agaaaatgtt tcaacactgt ctctgcctgc ttggcaatca gccacatcta gtctaccttc
    87481 atattttcca gcataataat caggaattat gccattaaga agttttaaaa aggtagattt
    87541 acctgaacca gaaggtccac acaaaacaat gaattgtcca gatgcaattt gacaagtcgg
    87601 tacctgacaa gctggatttt tttgatcatg ataggtaaaa acaagttgtt ctgctgaaat
    87661 cattgaagta tcgtgatgat tatgataaca gtaatccaaa tctgcacccc ccaatctttc
    87721 catgttaagt gtgagctaaa gcattcactg gtgcctttgt tgactgctag ccctttagtt
    87781 agactagcga tagttagttc ttggctactt cttatcaaag ataaaagcaa tggaaccatt
    87841 aaatactcca agtattgttt aggtctgatc aaaatagacc attttgttaa aataatcccc
    87901 ctgattgtta aagagcgatg aataacacaa cattcttgtc taatcattgg tatgaaacgg
    87961 cacataacgg ctaaggtcaa tagccagact tctgggaaat gccatttgcg taatccatga
    88021 accaattcgt agatggtagt tgtcttaata gtaattaaac ctgctagtaa agaagggaaa
    88081 actaaccttc ctgctattga gacaaaactc aataagtgat caagataaga agggaaaata
    88141 gatagcggga ttacagatag accgatcatt aacagataaa caaccgccaa cttgaacgct
    88201 agctttttct tatttaaagc agacaaaagt aataagagaa aaataacaat tataaattct
    88261 aaaaagccat caacacgaaa aaagaagcag gcattggcaa gtaccaacaa aagcagtttg
    88321 gtgcgaacat caagcctcat gataacaatc ctgatttgat aaaatgtttc tttagtaaat
    88381 aatgtgcaaa agctgaccct aataaggctc ctgcaagaac aattcctaca aaccaaataa
    88441 catgccctaa actaaaatca tacataatgc gattgacata ggtcatactt ttaccgcggg
    88501 ctaacaaact ttcaacatag gcatctttca acaaccacat cagcaaaata gggccactat
    88561 tgacaaaagc aaaaaccata taagataggt cattaaaaaa ccttgacttg tattttccta
    88621 aagaagcaat aatatccgct attaaaccga aaataatgct gggaaacatc gcggcaaaaa
    88681 agtgaccact aaggaagaaa aaacctccca tcatcagtcc taataatgtt attggcccaa
    88741 atgtttgcaa tttagcaatc aaaagaaaat aaattgttcc ccccagtaaa gctgcaaagg
    88801 caggggcata taacatattt cctgagcgat caaaaaagat gttaataaac gtgcctagac
    88861 caacacaaat aaagtagagc gcggcaaatg cgcctgttat cataatatct tttattgtta
    88921 actgtttcat tttttattca cctcttttag gaaaccattt tttacgcaaa atagatgatc
    88981 agcacaagct agggttgatg gcctgtgaga aattaagaag accaatcctt gacaatgtgt
    89041 tttaattaaa tcaagcaaga tagcttcatt tagagaatct aaatttgacg tgggttcatc
    89101 aaaaatatag caactcgcat ctttcaataa agctcgcatc aactctaaac gttgaccttc
    89161 tccagctgaa aagtcagaag gactcttgat aatcgtatcc aaaccttcct tacaagctaa
    89221 cagtctgtct ttcataccac aagcctcagc taaattccaa atggactcat ctgaaatatc
    89281 ttgacggcca aaggtaagat tttcacggat agactgttgg aaaagctgcg ctgtttgtgg
    89341 cacataagca atagtatctt gtaatttagc agcatttagt aagcaactat tcctatcatt
    89401 aagaaaaata tctcctgtcc tccaattata ccatttcata attaatttca ccaaagttga
    89461 tttaccagat cctgattcgc ctttaatgcc aataatcccc ttttcttgaa aggtaactga
    89521 taaatcctta aaaaccagct cttggcgctt tggataagca aaactgaggt cctcaaaaga
    89581 aacactcctt aaacgatcta tctgttttgt gccttcgtct actatgacat tttcgtctag
    89641 aaggtcaaaa atatttcgag ccgcattcat agcacgctta aagcctaatg gcaaacgtcc
    89701 tagttctaaa aaaggagaaa acgaagcagt gaaagcaata aaagttaata agcctttatc
    89761 aaatgataag ctgtcatggc atatcccatc aaaaaccata taagaaaatg ctaagatcat
    89821 taagccaagc cagaaaaaag tcaatgccat ttgcaaaaat tgagcttgcg ctacgttacg
    89881 atcaagagca tttacatgat tacttttttt tattaatcgt tcaaattgct cgtctaatac
    89941 ctgaaattgc aaaagatctt tgactgatcg taaactctct aaaaaataag acaggtaatc
    90001 ttttcgacct tcattttggc tttttagtaa aacctgtaaa atattagcaa aataaatggg
    90061 tataacaatg gctaaacaag cataggttgc caaagctaat aaggctaact gccaactact
    90121 ttgacaaaag taccagaaca ttaaacctgc agacaatatt gctgtacaaa taggcgcgat
    90181 tgtgtgggca aaaaaaactt ctaatgcctc aatatcttct ccaatcattt taagcaaata
    90241 accactgtct tgactatcca agcaagctgg agaaagagct cttagtttct taaagatcaa
    90301 gttcctaaaa gctgctaaac tatgaaaagc aacaaaatga ccaaaataat gctctccata
    90361 acgaacaaaa cctcttaaaa aggctagagc aatcaaaaga tataaagcta atattggaat
    90421 cacttcttgt ctaatagcta gtagtcctaa ataagctatt ccagttggaa tacttaccgt
    90481 gatgacaaaa cccataacag caaagccaac agccaagata atccaaggta gcagagtttt
    90541 cattagggtt aataaacgcc aaaccaggct taaggtagat ctgttaggtt tagccataaa
    90601 tacttgcctc cagtcttgct tgagtatcaa ctaggtgacg ataagttggt tggtcccgat
    90661 aaaggtcgca aggtttgcct aaacaagcag gctgccctgt gcttaaaaag agtacttggt
    90721 tagctccttt cagtcgagac atcttatgag taatcacaat aactattgcc gtcttagcta
    90781 atctcgttat taaattgtca ataatacgtt cattttctgc atctagacta gatgttgcct
    90841 catcaaagat ataaagagac ctcttactta atagagctct tgcgcaaatc acctgttgct
    90901 tttggcctgg tgataacagg ttaccatttt caccaactat tgtgtcaagg ccatctggta
    90961 gccagttgat gaagcttaac aaaccatgtt gatctatcca atctaagatt tcttttttag
    91021 agagatttgc ggctaacctt aaattatcgt aaatgctacg gttcaataag gtagattggt
    91081 ccgatacata aagaacctgc tggttgataa tttcttgcga caaattgtca ctgtctaaac
    91141 cgtcaaataa aatatgccca gtcgttgcag actggcgttt caagaggagt tgagctagac
    91201 ttgtttttcc ttgtcctgac acccccgcga tagctgttag ttggcccttc gttaatgtca
    91261 tggtaacccc actaagcact gtttttttct cataagctaa agaaatattt tggatgtcaa
    91321 tctggttaaa gtttattgct gttttactct tgttatcttt ccttgctgga acactatcca
    91381 agaaactaaa gatacggtca gccattttgg tattcatcat gaccaagtgc atgccataac
    91441 cctgctcacg aatcggtgta aaaaattctg tagcaatcaa gagaaaaaag aggaaattaa
    91501 aaaaagataa ctgacctgtt gccaaagctt gaacggctaa aaaacctgac aaaccaattc
    91561 ccagatacat aactgcatcc atataaccaa ctgcttgtaa ctgaaagcca agcaaagaca
    91621 tggtagcctt tcgaaactgt tctgccttac ccgaaaattc ttgctcgtaa cgttctgttg
    91681 cttgataaga atacagtgta ttaagacctt ttaagtcatc cataaataaa ttaccaacat
    91741 ccatataaga agaccaataa tgactcataa tttgcttaga ccgcttttgc atagcaacga
    91801 ttgaaagtgg aatcagaggc agaactccta aaaagacaag gcctgccaat ggataaatta
    91861 aaaagactag tatcataata gttgtgcagt tccacttggt tctcatagac aaagacaaat
    91921 agtaaccata ataagtatcc aaactatcaa tcccctgtga agccaacgtt aaaatatcag
    91981 catcactggc atgagcatca aattgcccgt ctaaatcaat aaaagcctca aagaaagatt
    92041 gtttgagtga atctctagca aattgtgaag cgatcccttg cagttgcttg cctaatatag
    92101 caagaacaaa gcctaccaca tttaaaagta ggagtaaacc tgccaagcgt cctaaggcaa
    92161 tagcatgacc caaaataaag gtcgaaaacg ttttggcaat gaggtaaaaa ctgatcatcc
    92221 tcatcacaaa ttgtaaccaa gataaaaagg ctgacacata aagcaaatac ctttttggtg
    92281 caattcgttc acgtaatcgt tttaacagtc ttttcctttt tccccttgat attgtatctt
    92341 ctgtttccat catctgttct tgttcctatc tctcctttga gttctaatat cattggtttc
    92401 attgaccaat gaagtgagga caaactgttg accatttctc tcaacaaagg gcaattcaac
    92461 accaaagaca tctctgataa actgttgatc aaggaggtct cttggttgcc cttgggcata
    92521 aagtcgccca tctttcagtc caataagatg gtctgaaaga gacaaggcct gattgatgtc
    92581 atgtaatact aacacaatcg ttaagcctag ctcttggttg attttagtaa tcagcttgag
    92641 aatgtcaagc tggtacttaa tatccaaata agtcgttggc tcatctaaaa aaataatatc
    92701 tgtatcctgg gcaagggcca tagcaatcca aaccctctgc tgttcaccac cagaaaggtg
    92761 ttggatgtca cgataaacat aatcactcaa agccgttact tctaaggccc aagcaaggcg
    92821 ttcctcatct tctttggtag tagttttaaa aaaagaacga cgggcaagcc tagccatggc
    92881 taccaattca cccactaaca aaccttcgac cacttgatgg tattggtgta ccactgccac
    92941 ttttttcgca aaagccttga gagaaatcgt tgctaattct tcctgctcaa gccaaacctg
    93001 cccttgtttt aacggaaggt ttttggttaa taattgtaat aaggttgact tgccactgcc
    93061 atttgccccc ataatagtgg taattttccc ttttggaatg ctaaaggtca ggtcatctaa
    93121 aacaagtttt tcctcataag caaaggaaag gttttgcacc tgaatatgac tgctgttctt
    93181 actcatataa tatcagacct ccttagcagg taaataaagt aaggcccgcc tacaatactc
    93241 attataatcg cagggctaat ttccaatggg taagcaagac ttcttcctaa agtatcggct
    93301 agcaaaaaga caaaggctcc aagcaaagct gaaaaaggaa ttaagatttg gtgcttactt
    93361 ccaaccagca agcggctcat atgagggaca atcaagccta ggaaagaaac cactccaacg
    93421 atcgaagtgg ctaccgaagc caataaaaca gcaaccaagg aaattcccaa ccgtaacgct
    93481 gtagcatcga ttcctaaatg acgaatcacc tgatcatcta aaagaagaag gttacaggtt
    93541 tttgacaata acaaagctag taacaagcca atgaatgtat aagggaataa aacccctaca
    93601 tcagcccatg ttttttgact aatatgacct gctaaaagag cattgaccat gggactagct
    93661 gaggtgaaaa aactggttaa agcagttgat aaccccataa ataaagcatt aatagcaatc
    93721 cctgttagaa taagccgaat aggattaagc cctttcttcc atgctaaacc ataaatcagc
    93781 aggaaagaca ctccgcctcc taaaaaggat acgattggac catacagcag caactgcggt
    93841 agaagtaaac tagagactaa aaccataaaa ctagcaccac tacaaatccc tataatagca
    93901 gggtctgaaa taggattttt caacactgct tgaaaaagaa ctcccgaaac agctatccct
    93961 gctcctgcta ataaagcaat gacaatcctt ggaaaacgca aatcataaat caaagccact
    94021 tgggggtcat aagccacaaa cagtcccttg acaattgccc cataagaaac cgccaaacca
    94081 ccaagggata aggcaataac cgccaaaaat aggaggctga gaattaaaag aactagataa
    94141 agaccacttc tgaaacgttt tttagccagt agggattgat ccctttgtaa atctgatgct
    94201 gtcatgttac ggatgatctc ccacgtggtc aaaaagctgt gttaaggtgt ccaaggcttc
    94261 tgggtagttc aatttagcac tcatgccaaa cagggtattg tccaaatcat agactttccc
    94321 ttccttgact gccgtaaaat gtttccaaat atcattttca gcaaattctt tgtcaaacat
    94381 cacttttacc ttgtctggaa tggcgtgagc tgttcgtaaa atcaagtcag gctccttagc
    94441 cagcatgtct tcaggattaa ctgatagaaa ttctttctca tctgactgat aaacattctc
    94501 accacctgcc aaatccaaaa gattccctac ataagattgg ttcgtcgcca ccaaataact
    94561 acctggcaag cccataagaa taagcacttt aggctttttc ttccccttac gtttagcttg
    94621 gaaagcacga taatagtcct ggtattgctg gcgcaattct tttgcttctt gttgacgttg
    94681 gaaaaggttc cctaaatcat caatggactg gtacatgccc tcaacacttc gtaagttcaa
    94741 aaaaccatac tcagtatcca atttttgata cttgggttcc aaatcttctt gtaaagaatt
    94801 gggactcaaa atccaagttg gtttcaaaga agcaatcaac tctatatcag gattcatggg
    94861 taaacccaca cgcttaacag catcatagcg tttaggaagg gtatataatt tactatcaca
    94921 aaccccaacg aggtctaaat ttaaacggtc acagatatca accacagcaa ccgaagtggc
    94981 tacaattctc tgctgttcag tctctttagc cgttttagag tgctgattca cacaagctac
    95041 caaaaagaag accattaagg ctagaccaat tcctttacat cgttttatca ttttttttcc
    95101 tctttttcca aacccataca gcaaaccctc ctaacatcag aaaaaaagtt ggaaagtagt
    95161 aaatcatgat tgataagtct tttttagacc gaaacttatc ttcttttttt aactctaaag
    95221 aagtcgttaa gcccatctta gaaggaagaa tttttttatc tgatagactc ttattatttg
    95281 atgagttggc tttaggctta ttctgagtta tcatagcacc tgtgtgagac tcatcttgac
    95341 tttctcctag ttttgtatca acaggctttt gactgtcttt tacttcttga ttttgagaat
    95401 tgtctgtttc agtaacaagc tgggccaaca tattacctga atacttttgt attaactctg
    95461 aagctgacag gtaaaagacc acttctctgc ccataggttc aacaaacatg cttcctcgaa
    95521 tgatactatt tacagtaggc aaactaatag caatatctag tgttgtccca ttggtatcag
    95581 tacctttttg ggtgatattg taatccactg cctgaaagct tccagttcct ccaggttgta
    95641 tccagaactg ataattgccc gaataatcag ctaagctcat acgaaaagta agaacgatct
    95701 ttccagcatc agatacttct agcatggcat cactatagac agtcccttca accatacctt
    95761 gaccaatatc aaaggaatgc tcccctccgc tatcttctat ctgcccagat atcggatgac
    95821 gataatttct ctgaataata catccataaa tttgaccttt atctgcatac acaacacctg
    95881 tcattgtcga taaacttatc atgaacacta ctattacctg caacaattgc tttattacta
    95941 cctttgtcat atgctcctct tgtctatctt ttcttaaaca tcttatttca ttttttaaat
    96001 gaatcccatt ttataacatc tccaaatgtg aaaaacacac ctatcttgcg actacttcaa
    96061 cctgtatcag gagtcctgtc ggaaataggt atgtttttag ctcttgctga ctagcttttt
    96121 tgttatttca cttggtacta agtttcttat ttaaataatg tctttcgacc aataatgaat
    96181 cccagagcta cgatcataaa gctagataga cctgcaatta agtgataacg agagctactt
    96241 tcttcaccat ttgattgttg attggaacga tgtttcttat cagatttttt agtcttagta
    96301 tcttttgccg ttttaccttc aattgatgtt tgatgatcac gcactaactg cttcaatttc
    96361 tctgccttat cagtatcatc tgtcttagaa gtttcttgag ttgattggcc ggttgctttt
    96421 ttagccagga gatcagttaa gttagcaata ccagtagctg ctgaaggagg acttgtaggt
    96481 gttttttctt gttgacgatt ttgaggtcct ttcaactgat ctgttgaagt tagatggcta
    96541 ttttcttgtg gattattagt ttctttgaca acctttgcct tctctgttgt aaggcttgcc
    96601 cagtctaaac gcataaaaac ggtttgaagg ccagaccctt ttgaaatggc atccatgata
    96661 ggcacaaaga cctgtaatgg cacaagtccg tctttcttcg cttgttcaat caatttcata
    96721 cgcagcactt ttggatagtt ttttccataa cgatctgcca atggtaggcc agttacatcc
    96781 gtgaaataag acacaacctc tgcctttttt gttcgaccaa ctggtttacc agctaaatct
    96841 ctttggtaac catctacgaa atagctcaat tcaccaaggt agcctaacat tttaccaacc
    96901 tttatacctc taaactcaac ttctaaatag gaaacacctt ttttaactac caaagtcact
    96961 cgatgattaa tagccttgtt tgacatggac tcagatgcta agtcagtttt taacatgctg
    97021 gcatttaaat agtaaatacc atcacgcaat tgtttttgat ttaatgctct aaggccgttc
    97081 tcaactaaat ctttctgatg tgaatgtggt cctttcgcca caaggatatc attagatgtc
    97141 aaatagatat gttctttttg agtagcaaat ttctttgtta ttaaagcttt gcgaatccga
    97201 tatgtacctg gatcttttaa agaaaactct cccgttaagt catctttcgg atcatttgag
    97261 agaagctttt cgtcaaccgt cacatactgt ccattggaag attttttttg caactgaatc
    97321 acaatactcc aatcgacttg catatcatta agtaaagaaa cagtatctga acctttttct
    97381 tgtaacaatt gttgatattc ttcaatagac actagttctt tatcaatggc agaatttgtt
    97441 ttttgctccc aataatacaa atctaaaaga ctgaaagctt gatgatattt tattttttcg
    97501 ccatctaaag aagcttcaag ttttgcaata tgtttttgtg gagtcagttt gtgttgtccg
    97561 ataagagctc ttacttttgg actcacactt tttatctgat tgaacttcac atctaaatta
    97621 agcgtttcaa catctggaaa tactgattct tcaacctgag taagcaagtt attgaaaaaa
    97681 tcaagttttg ttaattggtt attctgttta aatataccat ttggaagatt tgaaatttca
    97741 ttagttgata aatcgagtac agataattta ggaagatttt taaatgaaat attatcaata
    97801 cgagtaatat gatttctact tgccacaata gtgcttaagg cgcgcaattt ttctattgtt
    97861 tttggcagta gtgctaaatt attttgagat aagtcgataa aattaagtga tgttagaggc
    97921 tctagcgctt tttcaggtat ctcctcaaga ctattttctg cgaaacctaa tgaagtcaga
    97981 cgagacaatt cttcaaatgg gttatcacaa agtcggccaa tacgattctt ggaaagatca
    98041 agttgctcca ggtgatgaag cgactggaat agtcctcctt caagagagtg aataaagtta
    98101 cttgataagt agagttctct taattgagca ttgcttttaa atgtatcttt gtctagataa
    98161 gttaaatgat tacttcttaa gttaagaaat cgaagttgct tcatttgact aaataactct
    98221 tttggaattt ccgtaatttg gtttccatcc agatgtagtt ctgtaatatt tttagcatgt
    98281 ttcaataaag aaatatctgt taactcgtaa tggtctagac gcaactcacc tcgaatctgt
    98341 tctaattccg tttttgataa tttaccatcg ccgtctaaat ctttgccaag tttaactaaa
    98401 actttttcaa gacgttcagt cccaacccct ttttctttat catcagtttc tacctgataa
    98461 ccactccaac ctttggtaac ggtcttacta aaggtcatgt caagtttggt gtatttgtca
    98521 tagtggttta aatcactttc ttgacctccc atggctgata ccaaaacagc acctttgtgt
    98581 aatttatcca aatcatcaat aggcatggta aattcgcttc gaatatagct accattgata
    98641 tctttttggc caacttgttt acgcactgct gctgggtagg tccctttgct ttcaatagaa
    98701 aagtcaatca aaaattgtcc caaagcagta ttaagcatgg aaatttccat tgtaccatct
    98761 gctttaacca ctaatttggc tcttttatca aaagcacctt gaagcatgga ggactcttct
    98821 gagttttctt tattagcttt aaagtttagc gtataggttc cttctgtgag attgttaata
    98881 gaaacctcac ctgcttgata agactcgatg gtatctttta acagtcgatg agtatcggcg
    98941 agtagcttac tatctttgat ttctttgtaa gaatccttga tgacttgaag tttctcagcc
    99001 agatctttga ccgcgtcttt gttgctttct ttagcaataa tcgcatccgc ttctttgata
    99061 gctttttcca aagtggtgac atccaacttt gttgattgtc tgtcttcagc agtcacatgt
    99121 tgtttttgtt tggcagatac aaaatggatt tgaccatctt tcttggtaac ggtaatgata
    99181 acgtcttcaa atccttttga ggatatcacg attgtatttt ctccatcttt gaaagctttt
    99241 gttccaagat gaagccctgt catattcgag acccagccat tagtatcttt tacagaatct
    99301 gaaataagat ctttataaac tgtaccattg actgtaacct ttgtgatttg ctgagtcaat
    99361 gcttttatct gtttgttatc agaataacga tcaatttctg gaaataaaat tgcaccatct
    99421 tcttgactca attttgttat taatgacaag ttagcagtat ttttcccaga tttaacaact
    99481 ttttgtgtgc cttggttttg tggcactggt ttatcactga gcacatcttt ttttaatgaa
    99541 tcctgatgat ctgttgttcc taatttttga gctgaaataa aggtcacttt atcgcccttt
    99601 ttaataaagg taattaggat atcaccatct tttttattaa caatagtcaa ttcatgctta
    99661 ccgtctttaa taaggttagc attgatctta atcccttggt agttaatatc aaaggttcgc
    99721 tcgccttcca ggcttttttc atagacatca ttatcaacaa taaccttttc aatatgttta
    99781 taatactctt ttccagctga ttgactagcg taagggccta tctcttcaac taaaagtcga
    99841 ttatcctgag ttaaagctac tgttgtcacg agttgtgact gaactaaagg ctcttgtgat
    99901 tttacagttt gagtagcctg aatgagaaca agggtagata ttgccacaaa cgcacatttg
    99961 ctgatttttt tcattttcaa tcaactttct aaaaattttt atgaataagc taactttttt
   100021 attaactgaa cttaatctat cactttgtga ttattttgtc aagattgttt ccaatggtcc
   100081 aattgtctgt taaaaagctc cactatgtga gccaagacac tagcaataag cctgaattga
   100141 taatgaggat atctctgata aaagaattcc tctacaacaa tattactgag ataaagctag
   100201 ccttaggata gttattacta gggctgattc aatcattact tcattcacaa aacatgtaac
   100261 acgttataag ctccataatt gtgttaggag acaacatgag atgtcactac tgcatattca
   100321 tcacaataaa aaaagacaat agccttaatc gtgctattgt ctcaaaatca tttatttact
   100381 tgaaactttg tcgtgttaca ccaacagttt aaaaggccta actcccgcta agaaaattaa
   100441 gccgtaagaa aagtcattag ttattagttt ttactcttgc caataacata tgttagctgt
   100501 tcagcctgct gagctgtaaa ggtacgataa gaaatcttac ctaagccgat acagttagac
   100561 tctgaaataa gaatagaccc atcttttcta acatcttcta caattgatac gtggccataa
   100621 gtgccatcag cgcctgcttg cccgggtgca aatgaaatag cataacctac ttttggagtc
   100681 tttgacaggg cataccctgc tttatacttc caatctccac catttcccat aaaaggatca
   100741 aattggtaac ccaattcttt ggcgcgatta tacacatacc aagtgcattg tccccaagaa
   100801 taactcgaag cttgataacc caagacatca taactcttag agagcttata acctgctggc
   100861 acagatgcaa aactagctga tgactgcact ttcaattcgg ctggaatggc tggtgtgcca
   100921 ccatggtcat caatccactt atccaggtct tccataattt gtgcgcgacg tttaccacta
   100981 ccagtagtat cagtaaaata aacacccccg tcacttgcaa agtttaattg acctcgtgaa
   101041 aacttagccg cttttaaatc ttgaagtgca aaattgctat ttttgttttt aataatggta
   101101 tcttgtgtca ttttgacaat agcactatca tcattaaact tacttgcttg agtcggatct
   101161 agatcaaaag ctgcatagcc aaacatatta gctccaagca aagtcgcaat cccttgagtt
   101221 cctaaagaac tctcagacat cgcaatagct acgatagctc gaacatccag cccacttttc
   101281 ttttcccatt gcaatagctt ttcaccatta atacgtgtgc tgtcataggc aatgcctgtt
   101341 gattttaagt aactatcaat ctggtcagct ttaataccat aacggcgaga taaaagatgg
   101401 tgggtatagg catttttacc actccaatgc tctacatatg ccgcgtatgt gttgacagaa
   101461 gggatatcta tctcactcaa atctaaatct gatacgtaag gtgacaacga ctgaatagga
   101521 gttcttacct ctggcaactg tctcgacaat gaagaagcag gtgccgtatc aactttagat
   101581 ggtgatggtt tgggctgatc agtcggtttt ggctggtcag ttggtttcga atgatcagtt
   101641 ggtttcgaat ggtcagttga tttatcatct acctggtttt ctgcttctgc cttgttgtcc
   101701 gaagaaccta cagcagactg actagaatcg cttctttttt tatcatccgc gttaacaaga
   101761 ctattagagt ttgtttctgc agaagcatca aggggacctt ggtcagtttt atctgatgtt
   101821 gtaggtgctg catttttatt attttcctct tttgaagaag aatcactggt ttcatttgta
   101881 gttgctttat gcgtctcaag cattacatct tgagccaaca ctgtcatgct ttctgctgtc
   101941 ggggctaaga gacttag
//
LOCUS       DAUCWO010000002        70032 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUCWO010000002 DAUCWO010000000
VERSION     DAUCWO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70032
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.002"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(6..569)
                     /gene="thiT"
                     /locus_tag="VGY20_000100"
     CDS             complement(6..569)
                     /gene="thiT"
                     /locus_tag="VGY20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ4272992.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     regulatory      complement(614..710)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(837..1685)
                     /locus_tag="VGY20_000101"
     CDS             complement(837..1685)
                     /locus_tag="VGY20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ4272993.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKASTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     gene            2010..2513
                     /locus_tag="VGY20_000102"
     CDS             2010..2513
                     /locus_tag="VGY20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4272994.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVIMMLFRR
                     LIGEQRI"
     gene            2497..2880
                     /locus_tag="VGY20_000103"
     CDS             2497..2880
                     /locus_tag="VGY20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ4272995.1"
                     /translation="MNKGFKIGFLALVAMLLVACSQGTKQTQTPSVPKSDHHVRLVVK
                     EDTNTVDEKVSFEEGDTVLEILKDNYEVKEKDGFITAIDGIEQDMKANKYWLFKVNGK
                     MADKGANQITVKDGDSIEFYQEVFN"
     gene            complement(2926..3405)
                     /locus_tag="VGY20_000104"
     CDS             complement(2926..3405)
                     /locus_tag="VGY20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ4272996.1"
                     /translation="MPNLYDFTVKAQDGSDISLVNYKEKVVLVVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFAKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(3398..5197)
                     /gene="pepF"
                     /locus_tag="VGY20_000105"
     CDS             complement(3398..5197)
                     /gene="pepF"
                     /locus_tag="VGY20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ4272997.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEEALQDIDLFKRN
                     YEDRLTSVDDFTQALIEIEHIYTQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMDDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYTSVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            5356..8154
                     /gene="ppc"
                     /locus_tag="VGY20_000106"
     CDS             5356..8154
                     /gene="ppc"
                     /locus_tag="VGY20_000106"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ4272998.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQTIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINHQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMNSSVGLNTSAWPSPASLEKPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     SNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            8294..9598
                     /locus_tag="VGY20_000107"
     CDS             8294..9598
                     /locus_tag="VGY20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ4272999.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            9952..11148
                     /gene="tuf"
                     /locus_tag="VGY20_000108"
     CDS             9952..11148
                     /gene="tuf"
                     /locus_tag="VGY20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ4273000.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            11390..12148
                     /locus_tag="VGY20_000109"
     CDS             11390..12148
                     /locus_tag="VGY20_000109"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ4273001.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(12247..13482)
                     /locus_tag="VGY20_000110"
     CDS             complement(12247..13482)
                     /locus_tag="VGY20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ4273002.1"
                     /translation="MALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTKQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(13469..14695)
                     /locus_tag="VGY20_000111"
     CDS             complement(13469..14695)
                     /locus_tag="VGY20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ4273003.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVALSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLGIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGESSENLYAGMDDNYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(14695..15504)
                     /gene="yidA"
                     /locus_tag="VGY20_000112"
     CDS             complement(14695..15504)
                     /gene="yidA"
                     /locus_tag="VGY20_000112"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ4273004.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFMSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(15959..17260)
                     /locus_tag="VGY20_000113"
     CDS             complement(15959..17260)
                     /locus_tag="VGY20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ4273005.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPLFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            17342..17728
                     /locus_tag="VGY20_000114"
     CDS             17342..17728
                     /locus_tag="VGY20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ4273006.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            17958..20639
                     /locus_tag="VGY20_000115"
     CDS             17958..20639
                     /locus_tag="VGY20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ4273007.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQANERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(20723..21718)
                     /locus_tag="VGY20_000116"
     CDS             complement(20723..21718)
                     /locus_tag="VGY20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4273008.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(21782..23689)
                     /locus_tag="VGY20_000117"
     CDS             complement(21782..23689)
                     /locus_tag="VGY20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ4273009.1"
                     /translation="MSLAFARFKETVNPDFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLSDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSLCLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQIDSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGILLIIDTIQAQGNHCLVSQFILDNHLDVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(23776..24597)
                     /locus_tag="VGY20_000118"
     CDS             complement(23776..24597)
                     /locus_tag="VGY20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4273010.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFFPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            complement(24584..25366)
                     /locus_tag="VGY20_000119"
     CDS             complement(24584..25366)
                     /locus_tag="VGY20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ4273011.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWSFAILGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(25385..25873)
                     /locus_tag="VGY20_000120"
     CDS             complement(25385..25873)
                     /locus_tag="VGY20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ4273012.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQNIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(25909..27108)
                     /locus_tag="VGY20_000121"
     CDS             complement(25909..27108)
                     /locus_tag="VGY20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ4273013.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKIYE
                     AAMHSMLRALIKDYANKDLKPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(27108..27545)
                     /locus_tag="VGY20_000122"
     CDS             complement(27108..27545)
                     /locus_tag="VGY20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4273014.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            27881..28675
                     /locus_tag="VGY20_000123"
     CDS             27881..28675
                     /locus_tag="VGY20_000123"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ4273015.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDRGMAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQSDGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            28700..29341
                     /locus_tag="VGY20_000124"
     CDS             28700..29341
                     /locus_tag="VGY20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ4273016.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVTYLKSVLEQ"
     gene            29370..30371
                     /locus_tag="VGY20_000125"
     CDS             29370..30371
                     /locus_tag="VGY20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ4273017.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLD
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGIRHFHFSGISLALGKKTQDLIEVLVREAKKRDI
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADATMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDVQR"
     gene            30376..31011
                     /locus_tag="VGY20_000126"
     CDS             30376..31011
                     /locus_tag="VGY20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ4273018.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGVSLDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            31308..31958
                     /locus_tag="VGY20_000127"
     CDS             31308..31958
                     /locus_tag="VGY20_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ4273019.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITADYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            32655..33779
                     /gene="queG"
                     /locus_tag="VGY20_000128"
     CDS             32655..33779
                     /gene="queG"
                     /locus_tag="VGY20_000128"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ4273020.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGFDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKDEDSQKELELIRHKWQF"
     gene            33848..34946
                     /gene="prfB"
                     /locus_tag="VGY20_000129"
     CDS             join(33848..33919,33921..34946)
                     /gene="prfB"
                     /locus_tag="VGY20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ4273021.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMNGEINGFIDAYLKWRIED"
     gene            34965..35657
                     /gene="ftsE"
                     /locus_tag="VGY20_000130"
     CDS             34965..35657
                     /gene="ftsE"
                     /locus_tag="VGY20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ4273022.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            35650..36579
                     /locus_tag="VGY20_000131"
     CDS             35650..36579
                     /locus_tag="VGY20_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273023.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(36888..37586)
                     /locus_tag="VGY20_000132"
     CDS             complement(36888..37586)
                     /locus_tag="VGY20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4273024.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            37754..38518
                     /locus_tag="VGY20_000133"
     CDS             37754..38518
                     /locus_tag="VGY20_000133"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ4273025.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            38626..41127
                     /locus_tag="VGY20_000134"
     CDS             38626..41127
                     /locus_tag="VGY20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ4273026.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLGHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGPKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYNYDFWRVNYEKAKTARLLITNHAYFLHRVQDDKDFAR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRL
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            41462..42655
                     /locus_tag="VGY20_000135"
     CDS             41462..42655
                     /locus_tag="VGY20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ4273027.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            42676..44022
                     /gene="asnS"
                     /locus_tag="VGY20_000136"
     CDS             42676..44022
                     /gene="asnS"
                     /locus_tag="VGY20_000136"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ4273028.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            44437..45327
                     /gene="rapZ"
                     /locus_tag="VGY20_000137"
     CDS             44437..45327
                     /gene="rapZ"
                     /locus_tag="VGY20_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ4273029.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            45324..46301
                     /locus_tag="VGY20_000138"
     CDS             45324..46301
                     /locus_tag="VGY20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ4273030.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNENDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            46298..47209
                     /gene="whiA"
                     /locus_tag="VGY20_000139"
     CDS             46298..47209
                     /gene="whiA"
                     /locus_tag="VGY20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ4273031.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            47342..48739
                     /locus_tag="VGY20_000140"
     CDS             47342..48739
                     /locus_tag="VGY20_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ4273032.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYSYEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPCSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            48891..50438
                     /locus_tag="VGY20_000141"
     CDS             48891..50438
                     /locus_tag="VGY20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ4273033.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHERNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            50585..51307
                     /locus_tag="VGY20_000142"
     CDS             50585..51307
                     /locus_tag="VGY20_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4273034.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKMGKMPKTEILS
                     FEQYQVTPYLSGLLELDPDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            51327..52526
                     /locus_tag="VGY20_000143"
     CDS             51327..52526
                     /locus_tag="VGY20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ4273035.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEVYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKTLALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(52623..52883)
                     /locus_tag="VGY20_000144"
     CDS             complement(52623..52883)
                     /locus_tag="VGY20_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ4273036.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(52998..53939)
                     /locus_tag="VGY20_000145"
     CDS             complement(52998..53939)
                     /locus_tag="VGY20_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ4273037.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            54333..54782
                     /locus_tag="VGY20_000146"
     CDS             54333..54782
                     /locus_tag="VGY20_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ4273038.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            54958..55242
                     /locus_tag="VGY20_000147"
     CDS             54958..55242
                     /locus_tag="VGY20_000147"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ4273039.1"
                     /translation="MRLEEIRQEINSIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDTND"
     gene            55235..56497
                     /locus_tag="VGY20_000148"
     CDS             55235..56497
                     /locus_tag="VGY20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ4273040.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGVAVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNPYLRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLLAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            56612..56959
                     /gene="rplS"
                     /locus_tag="VGY20_000149"
     CDS             56612..56959
                     /gene="rplS"
                     /locus_tag="VGY20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ4273041.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            57206..57277
                     /locus_tag="VGY20_000150"
     tRNA            57206..57277
                     /locus_tag="VGY20_000150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57239..57241,aa:Arg,seq:cct)
     gene            complement(57240..57554)
                     /locus_tag="VGY20_000151"
                     /pseudo
     CDS             complement(57240..57554)
                     /locus_tag="VGY20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            57962..58531
                     /locus_tag="VGY20_000152"
     CDS             57962..58531
                     /locus_tag="VGY20_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ4273042.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            58532..60484
                     /gene="gyrB"
                     /locus_tag="VGY20_000153"
     CDS             58532..60484
                     /gene="gyrB"
                     /locus_tag="VGY20_000153"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ4273043.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQKFKRGAVV
                     ADLEIIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            60852..62576
                     /gene="ezrA"
                     /locus_tag="VGY20_000154"
     CDS             60852..62576
                     /gene="ezrA"
                     /locus_tag="VGY20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ4273044.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLLEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(62708..63166)
                     /locus_tag="VGY20_000155"
     CDS             complement(62708..63166)
                     /locus_tag="VGY20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ4273045.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKKTPEKTKSFWKRFLKK"
     gene            63393..64700
                     /gene="eno"
                     /locus_tag="VGY20_000156"
     CDS             63393..64700
                     /gene="eno"
                     /locus_tag="VGY20_000156"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ4273046.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAVLGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERGVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(65182..66211)
                     /locus_tag="VGY20_000157"
                     /pseudo
     CDS             complement(65182..66211)
                     /locus_tag="VGY20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(66478..68019)
                     /locus_tag="VGY20_000158"
     CDS             complement(66478..68019)
                     /locus_tag="VGY20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4273047.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKILCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHVVGPKYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYHDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNPFHKDKWT
                     PKKVKELFELVMTKTIGKTLKASLRPLLGNNILKSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
     gene            68491..>70032
                     /locus_tag="VGY20_000159"
     CDS             68491..>70032
                     /locus_tag="VGY20_000159"
                     /inference="COORDINATES: protein motif:HMM:NF019202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ4273048.1"
                     /translation="MFLKHQDVKQKNWRMRKVKKLFVSSCMLLTVGLGVAVPTGFSQS
                     NGVMVVKADVTQEGSPENVLKIANSLKATTDGTKIGRYSAINTQNLDVSEYKFYYVNS
                     TKLNKNPQQRLWHYEKPKEIYDSTIRVAGDGTANIRFYADGDGSKTPNGFSFAYKFNT
                     TNLKTDHKYKLSFKQTQNNDEEVYTKYLVGKASEGVANYLTSEETSVARNVELFEGNQ
                     KTTAERKFAKHQSGEKSLQFISKTDGTASLVLAAGVKNTPKKNTDITFDSFEFIDITE
                     PAQVSSGEAIAGNKNLTVNLSGSDGRKNFSGEIIEVYKNGQLLKKETVTTAGNKVNIA
                     LSDDVVLKKDDEITFKVKQGSSGKKNQAAGKIIVQQNPAVVAEQENSKKSLEEVYNKA
                     KKAISDDEWLSDEEKRVQTAAVEKTYNEGQTSINNAETVTEIKDAFKKYSTQGNKISI
                     PDQYKKGEKAIKQEQARKSLKQVHEDTLGKIRSDDWLTEEEKATQTENAKQSYNQGEN
                     KINQSD"
BASE COUNT        21397 a        12388 c        14790 g        21457 t
ORIGIN      
        1 aaaaattagt ctttgggatc aaataattga ggatgaattg gtaaagcaat cattaacgct
       61 aaacatgtta ttaaaaaagt taggacacca gctgtaccat taacgctaaa agaataccag
      121 acagcggaca tgcctttagg ggcgtaactg ccccagaaaa tgattcctgc aaggaagtgc
      181 cacaagtaac gtactgtaac ggctagtaaa ctagccatta ttgcaagaaa aacggcaaaa
      241 aaggtttgtt gtcttcttaa agttttaatc aaactatcag agaaactacc ggctaatccc
      301 atagaggtaa aggctaagat atattcaata aagacttggg acatgctgag atagtaaact
      361 tttccaagta caaagtgaag cagcccccaa atgagacccg ccaacatgcc gtactttaac
      421 ccacggcgca aagagaaaat aaccagtgca atagcaccat aagaaggact aaaccagcca
      481 gcaaaatctg gaataaatga aagggccatc gccaatgcag caaaaatagc agcttcgata
      541 aggtatttga cattggtgtt tggtgacata aaaaactcct tttgtgcaca aaaagagtat
      601 cgctttccaa tcattgatta ttggtcacaa tccctacgct tgtactaaca agatcaggtc
      661 ctcaggattt cgcaattgcg atctcagcca aagcactcct ttgtgaaata ttatagtgct
      721 ttcattatat gatgagataa tctccttgtc aaggataagg ggggttaaat tattgaccta
      781 gagtttttct tggtaagatg taaactacaa aaaagattag ccatgatgct aacctcttaa
      841 ttgttggagt tgccaaataa cttctcaaac gtttttttag tgtcttttgt tacggcaatt
      901 tgatttaaat ctaaagcatt gttaaaacca ctgagataac cttgtgaggt atattggtgt
      961 aaatcgtaat ctaaggttgt attaggagca gcctcataat agccagaatc actaccataa
     1021 gtcggaatcc atacagcatc gaaacctttt gttgaaatat cttgttctgc cataaaataa
     1081 gttccaatat agagaccaac attttcagca ccaagttttt taagctcttc tcggaaggct
     1141 gttacgcctt tattcatatc tttcatagtg gcttcttcga catcaatcca gtagtaagtt
     1201 ggattatagg gagcagcgtt tttgtagaaa gctcgggcct cttctttcat ctctttagta
     1261 ctacgaccaa gtgcgtaact gtaaacagct acaggaacat ttcgtttttg aaattctttg
     1321 atatgcgttt taaaagattt gtcaattcca gttgtaaaag cagcgttgtt atgagctgta
     1381 atttgcgatc ctccatagac acgtacaatt gcgccagaga tgtgacgtga caatgtgtca
     1441 taatcaattt cttcagggag ttgccagccg gaaacatcaa caataggatt taaaataaag
     1501 tcttcggttt cagaagtgct agcttttgct ttactaggat tactggttgc aataggaata
     1561 tgactcttag cgtcttcaac ttctttttgt ttcgcatgat tagcttgtct ttttccgata
     1621 atcaatacca ttgccagtaa aataaagaag actagcacaa caataggttt tatttttctt
     1681 ctcattaact gttattgtac cctaattact aaaaaaaatc aaaatatcct aactgttttc
     1741 ttatattgtc ttgcatttta aggtattctc tttgagaagt tacaaatgag atcctctcta
     1801 actatgtcaa gtttatgtca ctatctctta tagttggtag ctatattctt tacaatatta
     1861 gtttagtctt tgactatatc atgacagtca gcaagtactg ttttgttatt taattctcat
     1921 gttattatca tttgataaat aagtgagctc caaactggta aacgctttgt aaatctgtca
     1981 taatagagaa aaaaacgagg aagaagttta tgtcagctaa gcttatgagt cgcattgcta
     2041 tcatgtcagc cctatgtgtt gtgttgagaa tggtttttag cagcctgcct aacgtgcagc
     2101 ctgtgacggc atttttactc agttatcttc tttactttgg gttagccgag gctgtattag
     2161 ttatgatgtt gtgcttgttt ttatcagcct ttttgctggg atttggccct tgggtctttt
     2221 ggcaagttac ttgttttgtg cttgttcttt tgttatggcg ttttgtcttg taccctttgt
     2281 ctcagcaatt ccccaaatat cagcttggct gtcaggcttt cttggtcgcc ttatgtggcc
     2341 tcctttatgg ggtgctgata gatacttgtt ttgcgtattt atatagtatg ccttggtgga
     2401 gctatgtttt agcagggatg ccctttaata tagcgcatgc tttatcaact ctcgtctttt
     2461 ttccagttat tatgatgtta tttaggaggt taatcggtga acaaaggatt taaaatagga
     2521 tttttagcac ttgttgctat gctactagtg gcttgctcgc aaggaacaaa acaaacacag
     2581 actccaagcg ttcccaaatc agaccatcac gttcgcttag ttgttaaaga agacaccaat
     2641 actgttgacg aaaaagtttc ttttgaggaa ggagacacag tgcttgagat tctcaaagat
     2701 aattatgagg tcaaagaaaa agatggcttt atcactgcta ttgatggtat tgaacaagat
     2761 atgaaagcta acaaatactg gctttttaag gtgaatggaa agatggcaga taagggtgcc
     2821 aatcaaatta ccgttaaaga tggtgatagc attgaatttt atcaagaagt ttttaattag
     2881 taactataat ataaaataga cttggcacac caagtctatt ttatattaac caagaagtgc
     2941 tttaaggtct tcttcaatta gtttaggatc tgtctttgag gaatagcgtt taatgacttg
     3001 accattttgg tcaatgagaa atttagcgaa gttccattcg atgcgtttcc cgaggggccc
     3061 acttttttct tcttttaacc aggtgaacaa aggatcagcg tctttaccat tcactttgat
     3121 ttttgcaaag cgagggaaag tagtgtgata ggtcagactg caaaagtgat taatctcctc
     3181 agcatctcct ggagcttgat ttaaaaattg gttgcaagga aaatctaaga cctcaaatcc
     3241 cttgtcatgg tatgtgtcat atagtgcttg caaggcctgg tattgtgggg ttagcccaca
     3301 tttggtagct gtattcacga ccaaaaccac tttttctttg tagttaacta gtgagatgtc
     3361 actgccgtcc tgagccttaa ccgtaaaatc atagagatta ggcatgtgac atctcctcgg
     3421 tgtaattgat aatttggtca acggtatcac tcaagaattg aatggtatct cgaagaggct
     3481 tttcagtggc aatgtctgcc ccaatgagca ttgcagtatc aagaggcgta cggctaccac
     3541 ctgattttag gaaatcaagc cattcttttg ccccatctgg gttgttttta aggttaaggt
     3601 aaccagctgt tgacataaca agcccagcag aataggtata actatataag cccatgtagt
     3661 aatgcgcttg gcgcatccag gtaagagctg cgtcatcgtc aatgtcaacg gcatctcccc
     3721 agaagtctgt taagacctct ttcatcattg catttaattg gtcagctcca aaggtaccgc
     3781 cttcttcaat cagggtgtaa accttacgtt ggaaagccgc ttccaatagg tgagtgatga
     3841 agttatgaaa gtaggtgtct gttaaacggt gagcaagagc aaaacgtttt tgacgagggt
     3901 catcaaactg atgttccaag taatcactaa gcatcaattc attaaaggtc gatggcgctt
     3961 cgacgtaata agttgacata tgggtgttga agtaactttg gtgattatca gagaaaataa
     4021 attggccaga gtgtccgatt tcatgaatga gcgtgtagac atcagacatt ctgccagtcc
     4081 agctcattaa gacatacgga tggaccttgt aaggatcagc ggcataacca ccagaatctt
     4141 tattggcatt agcagcaaag tcaacccagc gttctgtctg ataacgttcg atttctttcg
     4201 tgtattcttg tcctaatggg gctaaagact tcatcacgag gtcataggca ccatcaatgc
     4261 tgacttctgg attgagatca ttgtcaatat ctagcttcca gtcagcaaaa gtcattttct
     4321 caagaccatt gacttgggcg acatgtttta agaatttctg agcaaccggt ccaaattcag
     4381 tcatgatgag gtcaatttgg cggtcgaaca agctacgatc cacttcctgt tcagctagga
     4441 ggtaatcaaa gacagaagtg tagcctttca tatcagctaa caatttttca gatttaacct
     4501 tggccaaata agcagcagca gcagtatttt ggtgtttacg gagacctttt gagaaggaac
     4561 ggaaagcttt gtcacgaatt tcagcatttt cgtgattttg atagaaattt tcgtaagaaa
     4621 caaagctatt tttataggtt ttaccatcca cctcaaaatc atccatgtca aagtcaccag
     4681 cccgcatttt ggtgtaaata tcatagggcg cattgataac ctcgcgtaag ttagccaaag
     4741 ccttttccac atcaggactt aagaggtgtt ctttttggat ttttgccatg cgaatagctg
     4801 cgctgaaata agggttcttt tctaatgtat ctaacacgtc aaggtcggca ttggctaagg
     4861 cggtgtcaaa gaagctaagg gcaacgctag ctttagtcat gaaatcatcg ccagcctgag
     4921 caatttgagc aaaactttca tctgaaaagt cagtggtttg aggcataaag gcataagtgc
     4981 caatatgact catttgagta taaatatgct caatttctat taaggcctga gtgaaatcgt
     5041 ccaccgatgt taagcggtct tcataattgc gcttaaacag gtcaatatct tgtaaggctt
     5101 cttcaatagc gagtaaaaaa tcttggcggt ctttgtagag cgctgttaga tcccaaagtt
     5161 cattttcagg gaattctgaa cgttttttca attccatagt tgtctcctta acaatatagt
     5221 caagactagt ataacagaat tttagtggaa tatggagact aaacctatcc aaacattttt
     5281 tgaaataaag cgcttacttt tgttaaaaaa caaacgttat gaacatgctc acttttattt
     5341 ttggtaaaaa gtggtatgat agaagaaact agcaaagaag gagatggtca tttgccactt
     5401 aaaaagttag agagtagtaa caatcaaact atcattgctg aagaagttgc tcttttgaaa
     5461 gaaatgttgg aaaatatcac acgacggatg attggagatg atgcgtttac agttatcgag
     5521 tctattatgg tgttatctga aaaacaagat tacatagaat tagaaaaagt tgtcgccaat
     5581 atttcaaatc aagaaatgga agtgatttcc aggtattttt ctattttacc cttgctaatc
     5641 aatatttcag aagatgtcga tttagcctat gaaattaatc atcaaaataa tacggatact
     5701 gattatttag gaaaattagc cctaaccatc aaggatcttg ctggaaaaga taatggtaaa
     5761 gacattttgg agcaggttaa tgtggttcct gttttaacag cacatccgac gcaagtacaa
     5821 cgtaaaacga ttctggaatt gacaactcac attcataaac ttctgcgcaa ataccgtgat
     5881 gctaaggctg gtgtgattaa tcttgagaaa tggcgtcagg agctatatcg ttatattgaa
     5941 atgatcatgc agacagatat tattcgcgag aaaaagcttc aggtgaaaaa cgagataaaa
     6001 aatgtcatgc agtattacga tggttctctc attcaagcag tgaccaagtt gacaacagag
     6061 tacaaaaact tagcccaaaa acacggtcta gagttagaca atcccaaacc cattaccatg
     6121 gggatgtgga ttggtgggga ccgtgatggc aacccctttg tcacagctga aaccttgtgc
     6181 ttgtcagcta cggttcaaag tgaggttatc ctcaattact acattgacaa attagcagcc
     6241 ctttaccgaa cattttcact gtcgtcaacc ttggtacaac ccaactcgga agtggaacgt
     6301 ttagccagtt tgtctcagga ccaatcgatt taccgtggaa atgaacctta tcgcagagct
     6361 tttcattata ttcaatcgcg tcttaaacaa acacaaattc aactaactaa tcagccagca
     6421 gctcgtatga atagtagtgt gggactaaac acatctgctt ggccctcacc agctagttta
     6481 gaaaaaccca tcttagccta tgactctcct gttgatttta aagctgattt aaaggctatt
     6541 gagcagtcgc tacttgataa tggcaactcc gctttgattg agggggattt gcgagaagtc
     6601 atgcaagctg ttgatatttt tggcttcttc ttagcaagca ttgacatgag gcaagactcc
     6661 agtgtccaag aagcttgtgt ggcagagtta ttaaaaggag ccaatattgt tgacgattat
     6721 agctctcttt cagaaactga aaaatgcgat gttctcttac aacaattaat ggaggagcca
     6781 agaaccttat cctcagcggc agttgctaaa tcagacctat tagaaaaaga actggctatc
     6841 tatactacgg cacgagaatt gaaagataag cttggagaag aggttattaa gcaacatatt
     6901 atttcacata cagaaagtgt ctctgatatg tttgagttgg ccattatgct taaagaagtg
     6961 ggattggttg accagcagcg ggctcgtgtg caaattgttc ccctctttga aaccattgag
     7021 gatttagata atgcccgtga catcatggca gcctatttaa gccatgacat tgtcaaatct
     7081 tggattgcaa cgaatcgtaa ttaccaagaa atcatgttag gctattctga cagtaataaa
     7141 gatggtggct acttggcatc gggttggact ctttataaag ctcagaatga attaacagct
     7201 attggtgagg aacatggagt gaaaatcacc ttcttccatg gtcgtggagg aacagttggt
     7261 cgaggaggtg gcccttctta tgatgccatt acttcacaac cttttggttc catcaaagat
     7321 cggattcgtc tgacagagca aggggaaatt attgaaaaca aatacggcaa taaagatgtg
     7381 gcttattacc atttagaaat gttgatttca gcttctatca atcgtatggt gactcaaatg
     7441 ataacagatc ctaatgagat tgatagtttt cgagaaatca tggatagcat tgttgctgat
     7501 agcaatacca tttatcgaaa attggttttt gacaatcccc atttctatga ttatttcttt
     7561 gaggcaagcc cgattaagga agtttccagt ctcaacattg ggtcaagacc agcagcccgt
     7621 aaaacgatta cggaaattac aggcttgcgt gccattcctt gggtcttttc atggtcacaa
     7681 aatcgtatca tgttcccagg ttggtatggg gtaggttcag cctttaaacg ttatattgac
     7741 cgagcacaaa gtaaccttga acgcctccaa cacatgtatc agacgtggcc ttttttccat
     7801 tccttgttat caaatgttga tatggtatta tctaaatcaa acatgaatat tgcctttcag
     7861 tatgcacaac tagctgaaag tcaggatgtt cgagatgttt tttatgagat tttggatgag
     7921 tggcaattga caaaaaatgt tattcttgct atccaagacc atgatgatct attggaggat
     7981 aatccttccc ttaagcatag cttgaagtca cgcttgccgt attttaatgt gttgaactat
     8041 attcaaattg aattgattaa acgttggcgt aataatcaac ttgacgaaaa tgatgaaaaa
     8101 ttgatccata caactatcaa tggcatagcg actggtttac gtaattcagg ctaatgccct
     8161 tgagtattaa ttggtattta tagaagtttt caagtgacta atcagaattt taaaggttag
     8221 cccttggagc ttcttttttt gaggattctt gacttttcct tttgtttgat atagaaagat
     8281 gttataatgc taaatgatta tctcaagaag tagagggaaa actatgaaga ttgataaaag
     8341 gcacctctta aattattcta tattattgcc ttatttaatt ttatcggtga taggcttaat
     8401 aatggtttac tcaaccacaa gtgtcagctt gattcaagca catgctaatc catttaaatc
     8461 ggtgattaac cagggagttt tttggataat aagtctagtg gctattacat ttatctataa
     8521 attaaaatta aattttttaa cgaatacaag ggtattaact gtggttatgt taggagaggc
     8581 ctttctttta attattgccc gcttctttac aactgctatc aaaggagcgc atggttggat
     8641 tgtgatcgga ccagtcagct ttcagcctgc tgagtattta aagatcatta tggtatggta
     8701 tttggcattg acctttgcta agatccaaaa aaacattagc ctttatgatt atcaagcact
     8761 aactcgtcga aaatggtggc caacacaatg gaacgattta cgggactggc gtgtttattc
     8821 tttgttgatg gtacttttgg ttgctgctca gcctgacttg ggaaatgctt ccattattgt
     8881 attgacagcc attattatgt tttctatcag tggcattggt taccgatggt tttcggctat
     8941 cctagtcatg attaccggtc tatccacggt ctttcttggg actattgccg ttattggggt
     9001 agaaagagtt gctaagattc ctgtttttgg ctatgtcgct aaacgtttta gtgccttttt
     9061 taatcctttt catgacttga ccgattcagg tcaccaatta gccaattctt actatgctat
     9121 gagtaatggt gggtggtttg gccaagggtt aggaaattcc attgaaaagc gtggctattt
     9181 gcccgaagca cagacagact ttgtcttttc agttgtcatt gaagagttag gtctaatagg
     9241 agcagggttt attttggcac ttgttttctt cttaatttta cgtattatga atgttggtat
     9301 taaagcaaaa aatcctttta atgccatgat ggctttagga gtaggcggta tgatgctcat
     9361 gcaagtcttt gttaatattg gtggtatctc aggcttaatt ccatctactg gtgttacctt
     9421 cccttttttg tctcaaggtg gaaatagctt acttgtctta tctgttgcag taggttttgt
     9481 actaaatatt gatgctagcg aaaagcgaga tgatatattt aaagaagctg aattatctta
     9541 tcgaaaggac actcgaaaag aaaatagtaa agttgtaaat atcaagcaat ttcaataata
     9601 ttctaaaatc tcctttaagg agattttaga atatagacaa tcccagtttg atgctcaaaa
     9661 aacttgaact gctctgctga tttcttattt ttgcgtgtga catgagccca aacagctgct
     9721 ttttgctgtg gcaaaaataa ggcgtggaat tccaaaggaa gtcgctgcta taagcaccac
     9781 ctaaggagag tatcgaaaaa gactttttct tctatttaaa aaataaagaa atggcaaaaa
     9841 acataaaata cttgcatttt cattgtaatt tgctacaata gtaaggtaaa gttagactgt
     9901 atggcctacc gtctatctat aaaatatatt ttattggagg cttttcctaa aatggcaaaa
     9961 gaaaaatacg atcgtagtaa accccacgtt aacattggta caatcggaca cgttgaccat
    10021 ggtaaaacta ctttaacagc tgcaatcaca actgtattgg cacgtcgctt gccttcatca
    10081 gttaaccaac caaaagatta tgcttctatc gatgctgctc cagaagaacg cgaacgcgga
    10141 atcactatca acactgcaca cgttgagtac gaaactgcaa ctcgtcacta tgcgcacatc
    10201 gacgctccag gacacgcgga ctacgttaaa aacatgatca ctggtgccgc tcaaatggac
    10261 ggagctatcc ttgtagttgc ttcaacagat ggaccaatgc cacaaactcg tgagcacatc
    10321 cttctttcac gtcaggttgg tgttaaacac cttatcgtgt tcatgaacaa agttgacctt
    10381 gttgatgacg aagagttgct tgaattagtt gagatggaaa ttcgtgacct tctttcagaa
    10441 tacgatttcc caggtgatga ccttccagtt atccaaggtt cagctcttaa agctcttgaa
    10501 ggcgacacta aatttgaaga catcatcatg gaattgatgg atactgttga ttcatacatt
    10561 ccagaaccag aacgcgacac tgacaaacca ttgcttcttc cagtcgaaga cgtattctca
    10621 attacaggtc gtggtacagt tgcttcagga cgtatcgacc gtggtactgt tcgtgtcaac
    10681 gacgaaatcg aaatcgttgg tatcaaagaa gaaactaaaa aagctgttgt tactggtgtt
    10741 gaaatgttcc gtaaacaact tgacgaaggt cttgcaggag acaacgtagg tatccttctt
    10801 cgtggtgttc aacgtgacga aatcgaacgt ggtcaagtta ttgctaaacc aggttcaatc
    10861 aacccacaca ctaaattcaa aggtgaagta tatatccttt ctaaagacga aggtggacgt
    10921 cacactccat tcttcaacaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    10981 ggttcaatcg aacttccagc aggtacagaa atggttatgc ctggtgataa cgtgacaatc
    11041 aacgttgagt tgatccaccc aattgccgta gaacaaggta ctactttctc aatccgtgaa
    11101 ggtggacgta ctgttggttc aggtatcgtt tcagaaatcg aagcttaatt ttaattaagt
    11161 tcccagaata gcaatttaag tcagacaact taagtttgaa gaagccccca attcgggggc
    11221 tttttgctat cttaaactcc ataaaatgct gataaataag aatgaaaagc tttcagaaaa
    11281 agagttttta tttgtgaaaa tattcctaaa atataagcaa ttccttttaa tcgtcggtca
    11341 tttgtgctaa aatagaagat ataaataaaa aaaagaagag gtatgtgata tgtcacgtaa
    11401 accaattatt gctggtaact ggaaaatgaa caaaaaccct caagaagcaa aagcattcgt
    11461 tgaggctgta gcaagcaaat taccttcaac tgaccttgtt gatgttgccg tagcagcccc
    11521 agctgttgat ttggtaacca ctattgaagc tgctaaagat tcagttctta aagttgctgc
    11581 acaaaactgc tactttgaaa acacaggggc attcactggt gaaacatcac caaaagtgct
    11641 tgctgaaatg ggagctgact atgttgtgat tggtcactca gaacgccgtg attacttcca
    11701 tgaaacagat gaagacatta ataaaaaagc caaagcaatc tttgctaacg gtttaactcc
    11761 aattgtttgt tgtggtgagt ctcttgaaac ttatgaagct ggtaaagccg ttgagtttgt
    11821 aggtgcacaa gtttcagcag cacttgcagg tctttcagca gagcaagtag cttcacttgt
    11881 tttagcttat gagccaatct gggctatcgg tactggtaaa tcagctactc aagatgatgc
    11941 tcaaaacatg tgtaaagctg ttcgtgacgt ggtagcagca gactttgggc aagaagttgc
    12001 tgataaagtt cgcgttcaat acggtggttc tgtaaaacct gaaaacgtta aagattacat
    12061 ggcttgtcca gatgttgacg gtgcccttgt aggtggcgca tcacttgaag ctgatagctt
    12121 ccttgctttg cttgatttcc ttaactaagt catctaaaaa agaaaaggtt aaatcctttt
    12181 cttttttaga tgacttaact cttactatga gagaataaag gtgattctac aggcaaatgc
    12241 attagcctaa tagcggttta atagcttttt tgccaatcta atgaaatgat atttgattgg
    12301 aaatggataa catctaaatg ttcctggttt ttgcaaaata aagccattga aattctgttt
    12361 aaagcgcaag acaccgtctg aaccatcaaa ttttccagta ataccaagaa aattatattg
    12421 tttgatgccc ttgtgaattg ccttgagcat agcatgctct tgtaatagag ctgggctata
    12481 aaatttatta aatttggggt aagatcctga aaagaggtag acggcctctt gtttggtata
    12541 gataaaaaga cttcccgcaa ggaacacatc tttagttccg tactcttcaa gaaaatggag
    12601 agcttcagaa attcttacct gaaaggtttc gaattggctg gatagttctt ttaatctatt
    12661 ttgctttttt tcagaatgag gatttttgcc aaggtctcct ttaactttgt tgatgctggt
    12721 tgctagttgt agttgtcttt gtttaaggtt attaaggtaa tcttcaaaat taagagttgc
    12781 aaccataaat tcacaagagt ctccaaaact atcataaaag tcctgatagt aagataatga
    12841 tttgtcaagg taatctctac gatcagaggt tgcttcagtg atttctttga aatggtgtaa
    12901 ctcatctcgt ttcagttgac gtaatttaat accaaaagta ttagcttttt ttattaaagc
    12961 ttttcctttt ttgctgaagg aatgggttaa acgtgaggaa tcaattcctt ctaacttttt
    13021 aacgtaatgc caaacaggtt caccctcagg atagcctgtt tttaaaccgt catgtttata
    13081 tcctaacgcc gttaaaaggc taataaggtc agtgtttgga tgagaaatag ggatgccatc
    13141 agtatcaaat gactgataat tgtcgtaagg ttttaaaatg agttcgataa cgtctttttg
    13201 tttggcatag tctttaagtt gagtgtaaaa atgttctaat tcttcgggat gattggtatt
    13261 agggcctgca ttgagttcca tacgccaacc tccagccact ttttggctaa ataccatagc
    13321 agcaaccttt agttcaccat ctttttcaag tccaagaaat tgtggttttg cccctctttt
    13381 agccatcaag ctggccattt ctgaggtttg gataaaggaa tgacggacga gactgtggca
    13441 gtaatggtca aattgttctt gtgaaatttc aattagtgcc attagttgtg ctccttaatt
    13501 tttttcttaa gttataggtc aagatggcta agcttgaaac aaggccgaca ggaatgttga
    13561 attcacctgc aaattcttcg atggtggggt tgagtctagc tttgaaatga tagagtccac
    13621 catcttgttg attttccacg cctcctaaat tatgccagcg gcagcctctt ttaaaagctt
    13681 ctttagccgt ttcataccag gttaaaagag gagcttgata attgcgataa ttatcgtcca
    13741 ttcccgcata aagattttca gaagactcgc cataaatcaa ggttagggtg gcggctagtg
    13801 ggatgcgggt tttgcctgca gcgagctgct cgcttaaaat ggttaaatct ttttgtagcc
    13861 gtgcaatggt tttttgattt tctgctactt tgctaggttt actcttatct gttaatcgtg
    13921 cttgttctgc taaggctttg tccaactgtt ggatcaagag ttttttttgt tcaggtaagt
    13981 ctaaactagc catagtaatg taggactgac cagcataaat acctagtaat ttttgatagt
    14041 aggatttgcc tcgaaggata atgccttttc gattctcggt ttttttcatc aaggctgaaa
    14101 agtcgtctag caattcactg ccaccaatcg tgacattaac acctttattg gtagcggtac
    14161 gaatggattg cttggctttt ttgggcaggc tgtcaaagtc aaaatccttg gcgtagatgt
    14221 tggcttgtat tcttggttga atagtgtctt cgagttcttt tgttctgcca gaccattcga
    14281 caccaagttt ttttagaaaa gcaataatag agagggccac atcattttct ttgctctctt
    14341 ttccttctaa agtttgtttg atgactaaac ttggatcaat tttgacaaaa agcgctcgtt
    14401 tgctttttcc gaaagttttg agcgttttaa tgacgaaatc aaggagttca aaattggcgt
    14461 agtccatgat tggcccacgt gggatataaa tcatagtgaa accaagagga agttttctga
    14521 ttaaacaagc agcagcagca acttgtaccc cattttcata aaagctgatg cgttcgtgtt
    14581 tccagttatc tttgactttt ccccactttg aactttgaag taagccagct tgtggctgtg
    14641 ctaagacaaa ctgatcatgt tcttcaggag atattccgat tttatatgta tacattagtt
    14701 taatacccac ttgcgaatag catgtgccac accactgtca ttgttggatt tggtgatgta
    14761 tttggctatt tttttcaatt caggaacccc attttccatg acaacagggt tggcaacgac
    14821 ttctaacata gcacgatcat tttcagcatc cccgatggcc atggtttgtg acatatctag
    14881 cccgagtttt ttagctaagt gtttgatggc attgcccttg ctgacggttt ttggcatgaa
    14941 ctctaaataa aaaggggttg acttgacgat ggtgtactta tcaaagaaat gttgaggaat
    15001 ttgcttgatg gccgcgtcca aaaggtcagg ttcatcaatc atcatcattt tgatgatttc
    15061 tttattggtc atttcttcgg gagtgcgata gaaaataggc atgtttacca aagtcgattc
    15121 atgaactgtg tattttccaa tattccgatt ggcagtgtaa ataccttctt tggtaatggc
    15181 atgcatgtga actcctaatt ttcggctcat aaattccgtt tccagataat cgtcgtaagt
    15241 catcaattct ttaacaattt cttcgccggt ctcggcatct tgcaccaagc caccattaaa
    15301 agtgataacg tggtttcctt tgtggttgag ttctaattgt tcgagtaatg aaatcactcc
    15361 ggcaataggt cggccagtag caatcaccac atgaacacct tgggctttag cctcttggac
    15421 agcttgaaaa acatcatctg taattcgacg gtcatcagtg agtaatgtgc catcaatatc
    15481 gacagcaacc agtttgatag acatggcatc ctccttgttt tttgtggttt atcttaaaat
    15541 tgttctccct cctatcttac taaaaatcgg tgcttttgtc atgggatggg aaaggaaact
    15601 taaggaaaaa cgagagaaaa cgttttagtt tagctcaatc gcctctgtag taactaaaaa
    15661 gattgaagaa agtactcttt cttcaatctt agaatggaga caaacccagt ttgatgctca
    15721 aaaagcatga actgctctgc tgatttgaca actgatttct tatttctgcg tgtgacgtga
    15781 gccaaaacag ctgctttttg ctgtggcaaa agtggtgtca atactggcag tgacgttacg
    15841 cggaattttt gagaccccag gctcaaaaat aaagcataga attccaaagg aagtcgctgc
    15901 cataagcatc acctaagaaa agtatcaaaa aagacttttt ctgcaatctt aaagggcttt
    15961 actgactaaa gtgaaaatgc ccattggaaa tataactcat aaaagtttcc ttgctaggag
    16021 caaagaggtc atcgagctct aacatttcct ttggaaaata aaagcgacgg tctccgtagg
    16081 ttgtccctgt taatgctttg acaataggag ataactgaga taattcagca aggctcccgt
    16141 ctttttgtat catttcaatc tgagttcttg gattttcgag ctctggtcga taaatatcat
    16201 aaggcaggtc gaagttgata tggataccgg tgtaataatc tggatcaaag ccaactgatt
    16261 cgaccaattg gcgcaagcgt tctaactctc cttgggaatc ttggtcaaag gtaacagact
    16321 tcagaatttt tcgattaata aagcgactgg ccaaatctga taagatatgg tcttcgcttg
    16381 ccatccagac ttggaagtaa gtattcatga ctccgtcatc tagggctata taatctgcta
    16441 aattagcctt cttttcaaag agggggatga gtccaggagc tgttttttgg aaataagcct
    16501 gttgctcagg atataagtgt tgggctcttt tgaggagatt ttgtaagatg agctcaacag
    16561 cacggctggc gggatgaaaa tagacctgca tgtacatttg aaaacgactg acaatatagt
    16621 cctcgacagc atgcatacca ctatgttcaa agacaatgcc atcttcaaca ggtcgaatca
    16681 cacgtagaat gcgcattaaa tcaaactgac cataattggc agcagaaaaa taagaatctc
    16741 ttaaaaggta atccatacgg tcgcagtcaa tctggctgga aatcagttgc acaacttgct
    16801 tgttaggata ggtatgatta atcacgctgg caactttgtc tggaaaatca agagcatgac
    16861 gcaccaaaat ggcattgatt tctgtctcag gattggtaat tatctcctga gtaaaggcct
    16921 cgtgatccgt atggaaaagg gcctcaaagg tatgagaata ggctccatga ccaatgtcat
    16981 gtaagagggc agccgtcata gtgaccagac tttcatcttt attccagata tcagcgtatt
    17041 tctcttcaaa aatagctgtc acccgacggg caatttcgta aacacctagg cagtgagaaa
    17101 aacggctatg ttctgcgccg tggaaggtga aagctgttgt agggacttgt ttgatgcgtc
    17161 gtaagcgttg gaattctttg gtattgataa ggtcataaat taatggatta tcaatgtgga
    17221 tatagttgtg aacagggtca cgaaatactt tttcattcat aatttttatt atatcaaaaa
    17281 ttgataaaat aaggagaaat tgatagaata aacagacgag cttagaatag aaagaagagt
    17341 tatgaaatta cagttaacca accatattcg ctttggagat gagacagaga ttattcaaga
    17401 aattcatgat tgtgagtggc gagaaaaagg gggctatcag tacctcatct atcaaaacac
    17461 tgacaaggaa aaggttgtca tcaagtacaa tgagacagaa ctgaccatga gccgtttttc
    17521 caaccctcag tcaatcatga aattttttgc gggcaagaag gtcctgattg ctctacctac
    17581 accgatgggg gttcaacaat ttttgacaga taccagtcat taccacttag attgttcttg
    17641 tcaaaaattg gaccttcatt atcatttgct tcaagctcaa acagaaatgc tatttgcaag
    17701 ctatcatctt gagctgagtt gggattaact aaaaagatag cagtaaatca tctgattagg
    17761 gattattctt gttttttagt caggttttta ctataatggt tattatatca agggagtagc
    17821 agacggctag ccgtgattgt atcgtcaata cggaatttcg gtttccggta caatcttaaa
    17881 cagcgagact tgtttaagaa aataaacagg tctttttatt tgaacaacta aaataaaaag
    17941 accaaggagg tactaatttg tctaaagaac aaagacatga agcattttac acgcaaagtg
    18001 aagagaccgt actggctcaa ttagagacca gtcgcgaagg tcttacgtct gcccaggcaa
    18061 acgagcgctt agcagagtat ggtcgtaatg agcttgatga aggcgaaaaa cgaagtttat
    18121 ttatgaaatt ccttgatcaa tttaaggatt tgatgattat tattttgatt gtggcagccc
    18181 ttctttcagt tcttacagag gggatggaag gtttgacaga cgccattatt atcttggctg
    18241 ttgttatcct aaatgctgct tttggtgttt accaagaagg acaagctgaa gctgctattg
    18301 aagccttgaa atcaatgtca agtccactgg ctcgtattcg ccgtgatggg catgtgactg
    18361 agatagactc taaagaattg gtccctggag atattgtctt gctagaagcg ggagatgttg
    18421 tccctgctga tctgcgtctt ttggaagcta actccttaaa aatcgaagaa gcagcgctta
    18481 ctggagaatc tgtaccggtt gaaaaagact tgtcaacggc tgtttcagaa gatgccggta
    18541 ttggtgatcg cgtcaatatg ggatatcaaa actcaaatgt gacatatggt cgtgggattg
    18601 gggttatcac taacactggt atgtacaccg aggttgggca tattgctggt atgcttgcta
    18661 atgctgatga gacagataca ccattgaagc aaaaccttga caatctttct aagattttga
    18721 cttatgcgat ccttgtgatt gcagcggtga cttttgctgt tggagtcttc ttgcgtggtc
    18781 aacacccgct tgaaggcttg atgacttcag tagcgcttgc cgttgcagct attccagagg
    18841 gacttcctgc gattgttact gttgtgcttt ctcttggaac tcaagtactt gctaaacgta
    18901 acgccattat ccgtaaatta ccagctgttg aaacgctagg atcaactgaa atcattgctt
    18961 ctgataaaac gggtaccttg acaatgaatc aaatgaccgt tgaaaaagtt tataccaacg
    19021 ggacactcca aagttcatca gctgatattg cttttgacaa tacgacactt cgtgtaatga
    19081 actttgctaa tgatactaaa gttgatcctt cagggaagtt aattggtgac ccaacagaaa
    19141 cagctttggt acagtttggg ttagaccaca attttgacgt acgtgaggct atggtggcag
    19201 aacctcgtgt ggcagaatta ccatttgatt ctgaccgtaa gttgatgtca actattcata
    19261 aacaagctga tggcaaatat ttcatcgctg ttaaaggtgc gccagaccaa ttgttgaagc
    19321 gtgtcacaca gattgaagaa aatggacaaa ttcgtcctat tacggatgct gataaaaaaa
    19381 ccattcttga taccaataag agtcttgcca aacaagctct tcgtgtcttg atgatggctt
    19441 acaaatattc tgatgctctt ccaacactgg aaacagaaat tgttgaggct aatcttgtct
    19501 tctctggttt ggtagggatg attgaccctg aacgtcctga agcagcacag gctgttaaag
    19561 tagctaagga agctggcatt cgtccaatca tgattactgg tgaccaccaa gatacagcaa
    19621 aagctattgc gaaacgtctt ggtattatcg aagaagatgg cgttgatcat gtctttacag
    19681 gtgctgaact caacgaatta tcagatgaag agttccaaaa agtctttaaa caatactctg
    19741 tgtatgcccg tgtatctcct gagcataaag ttcgtatcgt taaggcttgg caaaatgaag
    19801 gcaaagttgt tgccatgact ggcgatgggg ttaacgatgc accatctctt aaaacggcag
    19861 atattggtat tggtatggga attacaggta cagaggtttc taagggagct tctgacatgg
    19921 ttcttgccga tgataacttt gcaaccatta tcgttgcggt tgaagaagga cgtaaggtct
    19981 tctcaaatat tcaaaaaaca attcaatact tactttctgc taacatggca gaagtcttta
    20041 ccatcttcct tgcaaccttg tttggttggg atgtgcttca accagttcac ttgctttgga
    20101 ttaacttggt aactgatacc ctaccagcta ttgctcttgg tgttgaacct gctgagcctg
    20161 gtgtgatgaa acacaaacct cgtggacgta agtcaagctt ctttgatggt ggggtgaagg
    20221 aagccattct ttaccaaggt gccttccaaa ccattttggt gcttggggtt tatggttttg
    20281 ccttgatgtt cccagaacac acaagctacc atgatgtcca tgcagatgcc ttgaccatgg
    20341 cttatgtgac cctcggtttg attcagttgg tacatgccta caacgtgaaa tctgtttacc
    20401 aatccatctt tactgttggg ttgttcaaaa acaaattgtt caactattct attccagtgg
    20461 cctttgtagc attgatggca acagttgttg ttcctggatt taatcaattc ttccatgtga
    20521 cacacttgac gatcacacaa tggttagtgg ttattatcgg aagtctcttg atggttgttc
    20581 ttgttgaatt ggtgaaagct gttcaacgat cacttggtca agatgaaaaa gcaatttaat
    20641 agacgctaaa aggtttgaga caattgtctc aaaccttttt ttataatcgt tacaacgata
    20701 aggactattg gtatgcctga cattaaaaag ttgattcttg ccaattgact tgacagtcaa
    20761 aaatttgttg tttggcttct tgactgtgac cttcaatatc atcgattaaa atcttagtag
    20821 cctgttcgcc ttcttcatag gcgggttgga taatagttgt tagtgttggt gaagaaaatt
    20881 ttgtccactc aatgttatca aatcctacta gtccaatctc tggtatatta aatttaagag
    20941 acttcatagc tgtaaatact tttggaagag cccaacaatt ggggacaaaa accaaactct
    21001 ttttagtcag tgatccttgc aaaaactgag caattgcctc tgaagaagtc tgattttcat
    21061 caataatcat ttcttgatga gtaagatgat tagcttctaa cacatcaatg aagcctgaag
    21121 cgcgttcaat acgagtgcta agtaaattgg ggttacccgt gatcatgata aaatgttcat
    21181 aacctttatc gatacattgt tgaatggtat cataaacagc atcataatta ttagttttga
    21241 cccagtttgt gcgatgttcg taaagttggc tatcaaaaaa gaccactttt ttcttcttaa
    21301 tatcgataat gcgagaatac ttcctgaaat tagaggtagg ctgaatgata aagccatcca
    21361 ctccgaggtt cagcatcttt tcaatgagtt catcttcgcg agaagggtcg tagttgctgt
    21421 ttccgatgat aatttggtaa ccaaattcct gggctttgct ttcaattccc ttaacaattt
    21481 gattggaaaa actattggta atatcaccaa taaccacgcc aatgagttta gtagatttgg
    21541 cgtttagact tctggcagca atgcttggtt tataattggt agcttcaatc gtgcttttaa
    21601 tgcggttttt agtttcttca gacatcttgt caaacctacc atttaagtag aaagaaactg
    21661 ttgtttttga agttttcgct aattcagcaa tatctttgat ggttactttg cgctgcattg
    21721 catacactcc ttaaaaatag taatggttag tcatattata acataaggac tcgtaaaaga
    21781 tttagtgttt caatcgaata atctcattcg tgatatggtt gataacaacc acctttcctc
    21841 tgattttatg accatttagg agaactagtt tttctccttt aattaagtcc tcttggagaa
    21901 cgcagatact gtagtcaaac tgctttcctt ttaaatgcca ggaaagggcg gtctcaattg
    21961 ggttgcgttt gcctgtttga agtggtgtta agggagtaac tttggtatcg tcagggacta
    22021 acagggtact ggtgcaaccc ttgtctttaa agggttttcg tttgatgaat ttgtgactag
    22081 tcaggtattg gttgtatttt tttgacaaaa tcgtttcttc gatagtgaaa gggcagtcac
    22141 tgattagtct gaggtcacta aggtgatctg tctttacgtc taagtgatta tcaagaataa
    22201 attgactgac aagacagtgg ttaccttgag cctgtatcgt atcaatgata agcaaaattc
    22261 ctggtggtaa gatgagaatt tgtcggtcat gttggtagtt gttcctatcg gagaattgcg
    22321 tctggtagct accttcaata aagtaaacgt gtccattttg tcgaaggtga caataattgg
    22381 atttggggta agaaaggtaa gcccaggagc tatcaatctg ctcaggtaat tgttcttcta
    22441 agaaggcggt agaatgatgg ctagcgcttt ttagggcata gcgtaatggt tcttctttgt
    22501 aagtgtatcg cccagcatca cagaaaagtg gttgtccttt ataatagaga cagagactat
    22561 tttggtcgct atgggtatgg gaactaccga tgggcccatt tttgaaaaaa agataataat
    22621 tttcttgatt gatggtaatg tgcccggaat gttcaaagtg atgcgcaaaa gttggtaagg
    22681 tttggacggg taactgttca aaagtgtgga cacctttttc tcctagaagg agaagactat
    22741 cgatatcaag tgtgggaaaa gcagcagctt ttaagtgtgg ttcttcaagg atagtggctg
    22801 ctagtgttag aatatctctg gtatcggtaa agtcactgtc tcctaagggg atctgtttgt
    22861 ggtcagggcc agtcattttg aggagataat cactcatggc aagcaaagta ggtctaagct
    22921 gtggaagata atcagggact agagcagtta attccagaag ggctttgagt acttccacat
    22981 gatacatggt tgattgttca aattgactgc cgtcttcgag aatttggagt gcgatttgtt
    23041 gcgtcaattc ttttctagcg aaagctgttg ctgcagcaat atcaaggtca gaaccaaagt
    23101 aggcatcagc taataaaatc gcagtggttt gcaagattcc ccaattgctg agactgtatt
    23161 tatcaaggta gttggcatgg agaaagtgaa gttgtttttc cagggaagct aggatgagcc
    23221 tttcctcttg cttagttaag gcgtcagaaa gattaaggta aatcaaacat ttgacccaag
    23281 caaaacaacg gatgccagta tctaaggttc tagtagctaa ccccttaggg tctaaaggaa
    23341 tagctgattc aatccaattg aggatgaaac ttttggcttt taggagatac cgttcatctc
    23401 tttcaacaag gtagacgagg attaatgttt ggagataggt ctgtcgattt aacataaaat
    23461 tccactcagg atcatctgtg acagcctctt gccaagtaat gggatctaag tggtaaggga
    23521 tgtggcaggg ctccatatcc caattatctt caaataaaaa ggtattggtt aagagtaggt
    23581 cagctatcct ttttcgatca gcatagctgt cagcctgata gtctagcagg taattcctgc
    23641 aaaagtctgg attgactgtt tctttaaagc gtgcgaaagc aagagacata ggacatttcc
    23701 tttccaaaag aaaagagacc tcatcatgtg cttaggaact atttccctat gctcataaca
    23761 ggtctctgtg aaccattaag ctaaaatacc taaccagcta ccgataatac cgataagaac
    23821 agtgaggaga accaatttgt aggttgtcca ttttttgttt ttaataaggt aatacatgag
    23881 taaggtaaag agagctggta aaagtgcagg agcaatttta tcaagcattc cttgaatagt
    23941 cacaaatttt tgtgctgtat tggccgcatc caccttgcca gccgcaaaag taatgggaac
    24001 agttatttta acagaagtag ccgctagtcc agcgataacg gtaacgccga caatatttgc
    24061 catgcgcgac acaaccgcca tttgttcgct tagtttatca ataaagcttg tgccgacctt
    24121 gtagccatag agaccagaac cgattttaat agcggttagg ataatattca tggctaggaa
    24181 gaagaaaatt ggacctaaaa ctaaaccatc actggctaag gaagctgcga tcgttgaaaa
    24241 gagtggcgct aagcaaaatt gtgataagga gtcaccgata ccagctaaag gccccataag
    24301 tgccattttg atgtttcggg tttcttcttc aggacgatca ttttctaaca tgaccaagtg
    24361 cagactggtg atgaaaggaa agaagtgagg gttggtattg taaaactcac agttatcttc
    24421 taaagcttga tacaagccct ttttatcact gccaaaatgt tttttcaagg caggatagat
    24481 gacattggcg tatccgatac cttggtaatt actgtaatta aacccatttt gtaggaagaa
    24541 agctctcaga gctgttttga ggtaatcttt cttggttaat ttattagatc cagtcatctt
    24601 gttggcctcc ttggacagtt gttgcatcga cttgtttagg tttgttgtaa aattcattca
    24661 aggcgaaaat gataccaata atggcgatac cgatggttgg aatttggagg taggccgcac
    24721 aaacataacc aatcagtaca aaaggaatca actccttctt ggccataaca gataagatca
    24781 tggcaaatcc gatagcaggt aacattttac cagcgacagt caatccattg agcaagacag
    24841 gtgggatata atccactaag tgaaggagag tatccattga caaggcacct aataaaccaa
    24901 ggcctaatcc aaggatagca aaagaccaaa tggtcccatt agcagcgaat ttgaagcctc
    24961 taatattgcc tttttgcaat tgttttttag cggtttcagg agccccagca aaagcggtat
    25021 aggcgaatgt ctgaagaaat tggatggcaa ctgcgattgg tgttgataag gctagggccg
    25081 cttctggggt gactttacca gcactggtaa tagccatcaa ggtaccaaag ataccagggc
    25141 cgataggatt aggtggaacg gtaccaccgg cacccactcc aaatcccata taagctaatt
    25201 cggagatagc tcccatgcta agtgcagttg gcaagtcacc taagatcatc cctacaccaa
    25261 aagacagaat aatacaacgg ttggtgtaga tgccaagtaa cattccgctg aaacaaaagg
    25321 ctgtccaaag accaattaaa agtgcctgta acaaattgat atccatatca atctccttta
    25381 ttcattaaat ataatctaag atgtcaacgt cagaggcgct attgccagct ggagttgttt
    25441 tggtgttaaa aaccacttgg tggtcatggg ctaagcaacg aatagcagac ttgtctgttt
    25501 ctcccagcga aataaactgg gtaatcgcaa ccttatcatc agttttatga atattaccga
    25561 tattgatttc tgtgatggga acaccccctt caactaggcg tttggcatct tgcaaatctt
    25621 tgaccacgat gaagatattt tgagcaggac ttgctttatg aatgatgtca atcacttttt
    25681 gaatggagaa aaaacgaatg gcaatactgg atggaataac tgttttcatg agggattgtt
    25741 gaatcttatc ttcagaaacc gcatcgttag cgacgataac ggtgttgcag ttgaggaatt
    25801 tgacccataa ttgcccctgt ccgtgaatca aacgctcatc aactcgcgtc atgataatgt
    25861 ttggttgtgt cataaaatct cctttttaaa tgtaggtatc ggtgtttgtt accagtaagg
    25921 gttccagtct ttgtagaaac gaagcagagc ttcaagatag taatagtctc cccagatatt
    25981 tccctcgtca accccttttc cagagtgcca agaatagact ccgtgaagta gtagaggagc
    26041 gccaggcttg agatctttgt tagcataatc cttaatcaag gctcttagca tgctgtgcat
    26101 ggcagcttca tatatttttt tatcaggatc atgatctggt aaggttttta acatttcatg
    26161 aataccacac acggcaatgg ctgttgctga actatcacga gactgctcgc taccatctcc
    26221 aaaaattaaa tcccagtagg aaacctggtc tttaggaaga cggttcaaga aataatgggt
    26281 catgcccttg aagagttgaa tgagttcagg ttcttttagg aaacgatagg tcaaaggaat
    26341 accataaatg ccccaagctt gccctctggc ccaagcagaa tcatcgctat aaccttgtcg
    26401 ggtgacccct ttaacaggat cgcctgtttc tggatcaaag tagaaggtgt ggtaagcaga
    26461 ggcgtcatct cggatgacat gattggctga ggcgtagaaa tggttgatcg ccatgtcacg
    26521 gtaacggttg tctccagttt cttggctagc aaaaaagagt aattggatgt ttaggagaca
    26581 atcaatgatt aaacggtaat cttctttttt ccccagttct ccccaggcct gaataaagcc
    26641 gcctttgtct tgatagcgtt ggactaattt gtcggctgct ttcaaagcag cttctctagc
    26701 ctcaggtgtt tttaaaagtt tccactcagc catgcaagaa ggggtgtaaa gaaagcccaa
    26761 atcatggtga tctagctcaa tgtctcttgt tactcgatct aggaaagaaa ggtcatttgc
    26821 ttgagccaga gctttaatag catcatcccc actgtattcg taagctaacc aaaggcaacc
    26881 tgtccaaaag gcattggtcc attccgtgtt gtccataatg gcgtattggt tatctttggt
    26941 agctggcgtt ggaaaatctt ctttgaagta gtccatgtta agccgaactt gtttgagggc
    27001 taagtcaagg gcctgagtaa tgtcttcttg gcttagaaag tcttctttgg taaatcgttc
    27061 aggctgcttg ataggttcaa gagcaatggt ttttaaagat cgtgtcatta aatgcctcct
    27121 aactcatcgt ctgtcgcacc gtcttcttgc tgagaaagag ttttccagtc aacaatgcct
    27181 tctttagaac atgttataag tcctgatact aagtcgtcaa aagaagctgc tgtttgtctt
    27241 gcaaaactag cttcaatcag catggctaag ttaaggccag aaagaacatt acacgtcgtg
    27301 tttggaagag attccattaa ggttgcagcg actttaaaag gggtacctcc tagaaggtca
    27361 caaagaacta aggtttcctc ttctgggatt aaagcacgag ataattggtc ctgcacatca
    27421 gcggcggtca tctcagcagt aaaatcaatt gcagttacct tctcttgtgg tcctgcaatt
    27481 aactctaggg atgagacaat cccacttgca aagtgtccat gtcccataac aataatagca
    27541 atcatgataa aactcctttt tggtttaccg gtttacttaa aaatattgta gcgctttcaa
    27601 aattctttgt caatactttt ttaaaagatt tttgaaaaag aaaaagtgag cagttgtgct
    27661 aaaaaagaat caaaaatggt cttttttgtc actatttaat gtagaaaagt cttaatagct
    27721 attaagtata ggacaagact aaacaagaat gacagtttgg ataagagcac tcttttaaat
    27781 ttttgaaccg ttttcattaa aatgattgac aaaactagtt tactttgtta tagtcttggt
    27841 aaacaggttt acttaattta ataacaaaaa ggagaatcct atggaaaata tgttttcgtt
    27901 acaaggtaag attgccttga ttaccggagc atcctacggt attggttttg aaattgccaa
    27961 agcctatgct caagcgggtg caactattgt gtttaatgac atcaaacaag agttagttga
    28021 tagagggatg gctgcctatc gagaattagg gatagaagcg catggctatg tttgtgacgt
    28081 gactgatgaa gctggtatcc aacagatggt tagtcaaatt gaagatgaag taggtgttat
    28141 tgatattttg gtcaacaatg caggtattat taggcgtacg ccgatgcttg aaatggccgc
    28201 agaagatttt cgtcaagtta ttgatattga tttaaatgct ccttttattg tgtcaaaagc
    28261 tgtattacca tcaatgattg caaagggaca tggtaaaatt ataaatatct gttctatgat
    28321 gagcgaattg gggcgtgaaa cagttagtgc ctacgcggcg gctaaaggtg gcttgaaaat
    28381 gttaaccaaa aatatagcct ctgagtttgg agaagccaac attcaatgta atgggattgg
    28441 tcctggctac attgcaacac cacaaacagc tccgttgaga gagcgacaat cagatggtag
    28501 tcgtcatcct tttgatcaat ttatcattgc taaaacacca gcagcacgtt gggggacaac
    28561 agaagactta gctggtccag ctgtttttct agctagcgat gccagtaatt ttgtcaacgg
    28621 ccatattctt tatgtcgacg gtggcatttt agcttatatt ggaaaacaac cttaatcaca
    28681 cactagaaag agattttata tgaaaattgc attaattaat gaaaatagcc aagcggctaa
    28741 aaatggtatt atttacgatg cccttacctc agtgacagat aaacacggct accaagtctt
    28801 taactatggc atgtatggca cagaaggtga aagccagttg acctatgttc aaaatggact
    28861 cttagcttct attttattga ccactaaggc ggctgatttt gtggtgactg gttgtgggac
    28921 aggtgttgga gctatgttgg ccttgaacag cttcccaggt gttacctgtg gttttgcgag
    28981 tgagccaaca gaagcatatc tcttttctca aatcaatggt ggtaatgcct tgtccattcc
    29041 ttttgccaaa ggttttggtt ggggagcaga gttgaatttg accttaatat ttgaacgcct
    29101 ctttgcagaa ccaatgggtg gtggttaccc taaagagcga gctattcctg aacagcggaa
    29161 tgcacgtatt ttaagtgatt tgaaaaaaat tacttacaga gatttgttgg caattgtcaa
    29221 agacatcgac caagatttcc tcaaagaaac gatttctggc gctcatttta aagagtactt
    29281 ctttgccaat gcggagccgt cagaattagt tacttatttg aaatcagtac ttgaacagta
    29341 attccatttt gacgaggaaa ggaggtcaca tgtctaagtt attattggtt ggtgaaccgc
    29401 tgattcgcgt tagccccaat cagtttcagc ctttgaccaa tgcctgcgac gctcagcttt
    29461 ttttcggcgg ttcagaagtt aatattgcaa gaactctaga cggttttggc ttagaagcaa
    29521 ggctatttac ggccctacca gataatcctg ttggtcacgc ttttcatcag tttttgaagc
    29581 aaagcggcgt ggatatgtcc ttgacggctt ggcaaggtca tcgcgttggg ctatattacc
    29641 ttgaaaatgg ctttggctgt cgggctagtc aggtttatta tgaccgctgt ggctctagct
    29701 tttcggcctt agataaggat agtcttgatt tagctgccat ttttgaaggc attcgtcact
    29761 ttcattttag tgggatcagt ttggcgctgg gcaaaaaaac tcaggacctg attgaggtac
    29821 ttgtgagaga agctaagaaa agggatattt gtgtttcttt tgatcttaac tttagatcta
    29881 gtatgattgc ggtagctgat gctaaacgac tcttttctca ctttgctcaa tacgcggata
    29941 ttatttttgg catggaacct ttgctgttag atagtgacga ttttgacatg tttgatagga
    30001 ataaggcaga tgctacgatg attagagaac gtctggcagg actttatcag cgctatcagt
    30061 tacaagctat ttaccatacg gagcgttcaa atgatgccca aggcagtaat cgtttcaagg
    30121 cttatgccta tgatgggcag ttttacgaat cctgtgaagt gacgacccca gtcttgcaac
    30181 gggttggaag tggagatgct tttgtggcgg gtttacttta tcagttgtta gaaggtaacg
    30241 aaaagcagcg caaccttgat tttgctgtgg caacagctag cctaaaatgt acagtagctg
    30301 aagatcagct ctatcacagc gtccaacaag tagaagcagt cttagctaat caacgagatg
    30361 tccagaggtg atgatatggg aaaaatagaa atcttaacca aacttaaggc taatcggctt
    30421 gtcctggttg tacgaggaga gtctagtgag gaagctttag cttgcagcct ggccagtatt
    30481 gaaggtggca ttaagacaat tgaagtcacc tatactaacc cctttgctag tgaggttatc
    30541 ggtcaattag ctgaacgctt caaagaagat cctgaggtat tgataggtgc tgggacagtt
    30601 ttggatgatg tcactgcccg ccaagctatt ttagcaggag cacagtttat tgttggtcct
    30661 aatttcaatc gagcagtggc tttgatttgt catcgttata gcatacctta tttaccaggt
    30721 tgtatgacgg ttaatgaagt ggttacagca ttagaatcag gggttgatat ggtcaaaatt
    30781 tttcctggtt ctactgtagg gatgtccttt attcgggcaa tcaaatctcc tttgccacaa
    30841 gtagaggtta tggttacagg cggagtttct ttagataatc tcaaagactg gctagctgct
    30901 ggagtagatg ttttaggcat aggaggcgag tttaatcagt tagcgagcca aaaacaatat
    30961 aacctaatca ctaagaaagc cgctcattat atcaaaagtt tgcgacaata atctaaaaaa
    31021 cgtttttacc ttggctttag tgctagtaaa aacgcttttt tatcattcat tcaattaata
    31081 tgcttctgct tatgtttttc cagatagtat agatataaga gcagctaatt taacaagatg
    31141 agcctgtcgt ttgatatata ggatataaac tttttatgat caaacaaaag taataccttt
    31201 tgaattcgtt agtattgacc tgatctaaag tgatatcact tagaatgaac tagctgtaca
    31261 gcttttacag caacttatgc taaaatagaa aaaaaggaga aatgttgatg attaaaggaa
    31321 ttatttttga tatggacggt gttttatttg atacagaacc tttttatctg aggcgacgag
    31381 aagatttttt taagacaaag ggaattccca ttgatcattt gaactctaaa gattttattg
    31441 ggggcaatct ccaagaatta tggaaagagt tgttaggtaa aaatagggat gatgctatcg
    31501 ttaaggcaat tacagctgac tatgacgctt acaaacaagc gcataagcct ccttatcaaa
    31561 aactgttgat tacagaagtg aactcttgtc ttgaacagtt gaaaaaacaa ggtattaaac
    31621 tggctgtggc atcaaactcg aagcgtcagg atgttttgtt ggcgttggag acaacgcaaa
    31681 taaaagatta ttttgaaata atcctagcgc gtgaagatgt ttctagaggc aaaccttatc
    31741 cagatatcta taataaagca gtacaaaaac taggattaca aaaaaaacaa ctgcttgtag
    31801 tagaagacag ccaaaaaggc attgctgccg ccaaagcagc caatctgaca gtttttgcca
    31861 ttaccgacta ccgatatggc attgatcaga gtcaagctga tcacaagata gatcatttag
    31921 gacaactgtg tgtaaaaatc ggttgttttg gatcgtgaag taacgcaact aactgatcat
    31981 tgatgatact tcaagacact taagaatacg tctaggactt tctacagacc aataacaaga
    32041 atacgtttat gtgtttttac tgttttttta gctacctatt tacaatatga cactgaaaag
    32101 agtactatgt ttaagttatt atcaaaaaaa ccgtcgcata agatcaccta gaaaaggatt
    32161 atgatttttg tgatttggat gcgatcgtta gtcgttgacc atctttatgt ggcaggagat
    32221 atgtttaacc gtggtcatta tttggatttg tttttagatt ggctagtgag taaatataga
    32281 aggatcataa tactgctttg acaaaggcag tatctgagtc catgagttgc acggataatg
    32341 tgactcggtt ttggttagct ataataatat ttggaagtgt tatgctgctt gtcttcgtag
    32401 gtccattgat tatagtcaga cttactttag agctgagctg actgtctaga caagacgtga
    32461 tggagatgtg atttctgacc aagtagaggt aaccagaaaa gggactttta cgatgtaatt
    32521 gaatttactt gttgcattct tgtgcatgtt aagagagaaa acccagataa atcaggaagt
    32581 aaaatggtta tgtttgatta gaattgtcct tctttttcct tgaagtctta acttattttg
    32641 attatgatag tagtatgact attaaggcag aaattaaggc actcgccaaa gaaattggta
    32701 tctctaaaat tggttttacg acggcagata actttgatta tttggaaaag tcattgcgtg
    32761 cttctgtgga agaggggcgc aattcaggat ttgaacataa agtcatagag gatcgtatct
    32821 cccctgaaag gcttttagaa tcggctaaaa cgattatttc aattggcgtt gcttatcctc
    32881 acaaacttcc tcagcaacct caaaaaacac cttataaacg tggcaaaatt acacctagtt
    32941 cgtgggggtt tgattatcat tatgtggtcg gcgaaaagtt agacagacta tcaaaaggca
    33001 ttgaagagct gtgtcgagat tttcctttgc aacaaaaagc aatggtcgat actggagctt
    33061 tagttgatac tgcagttgcc caaagagcgg gtattggttt tataggaaaa aatggtctag
    33121 ttatttctaa agaatatggc tcttatatgt ttttgggaga attaattacc aatttagaga
    33181 tcgagccaga caagcctgtc gattatgatt gtggtgactg tcgtcgttgt ctagatgcct
    33241 gtccaacttc ttgtttaatt ggtgatggct caatgaatgc caagcgttgt ctatcatttc
    33301 aaacccaaga taagggaatg atggacatag agtttcgaaa aaagattaaa acagttatct
    33361 atggttgtga tatttgccaa atttgctgtc cttataacaa aggtataaat aatccaccag
    33421 ctacagagat tgatcctgag ttggctcaac cagaattaat accatttttg agtttatcaa
    33481 atggacaatt taaagaaaaa tttggtatga ttgctggaag ttggcgaggt aaaaatattt
    33541 tacagcgtaa tgccattatt gcgctagcaa atgctcatga taaaaccgca gtggtcaaat
    33601 taatagagat tattgataaa aataataacc ctattcatac tgcaacagct atatgggcat
    33661 tgggagaaat tgtcaaaaaa ccaaacgatg agatactggc gtttatgagt catttaacct
    33721 taaaagatga agatagccaa aaagagttgg aattaatccg tcataaatgg caattttaag
    33781 ccttttatgg tatttttaag aaccttatga taaaataaaa cagattaaca aagattggag
    33841 aaatctcatg gaagtggctg aaattcgcca aaaaatagta gaaaataaag agaagttgac
    33901 tagcttcagg aggtctcttt gacttagatc gtttggaaga ggagattgct cttcttgaaa
    33961 atcacatgac tgagccagac ttttggaatg acaatattgc agcgcaaaag acatctcaag
    34021 aactaaatga attaaaaggt aagtatgata ctttccataa tatgcaagaa ttatctgacg
    34081 aaacagaact cttacttgag atgcttgacg aagatgattc tttgaaagaa gaattagaag
    34141 aaaatctaat gcagctagat aagataatgg gtgcttatga aatgacgctt ctcttatctg
    34201 aaccctatga ccacaataat gctatcttag aaatccatcc tggttcagga ggaactgaag
    34261 cgcaagattg gggagattta ctcttacgaa tgtatacccg ttttggcaat gctaatggtt
    34321 tcaaagtaga agtattggat tatcaagcag gggatgaagc tggtattaaa tcggttaccc
    34381 tctcttttga aggtcccaat gcttatggtt tgttgaaatc agaaatggga gttcatcgcc
    34441 tcgttcgtat atcacctttt gattcggcta aacgtcgtca tacctcattt gcttcagttg
    34501 aagtgatgcc agagttagat gatacgattg aagtagaagt acgtgatgat gacattaaaa
    34561 tggatacttt ccgttccgga ggagctggtg gtcagaatgt caataaagta tcaacaggtg
    34621 ttcgtttgac gcatattcca actggaattg tggtatcgtc taccgttgac cgtactcaat
    34681 atggaaaccg cgatcgcgct atgaagatgt tgcaggctaa gctatatcag ttagaacaag
    34741 aaaagaaagc acaagaagta gatgctctca agggtgataa aaaagaaatc acttggggaa
    34801 gtcagattcg ttcttatgtt ttcactcctt atacgatggt gaaggatcat cgtactaact
    34861 ttgagcttgc tcaggtagat aaggttatga atggagaaat caatggcttt attgatgcct
    34921 accttaagtg gcgcattgaa gactaaaaat aataaaggag aaacatggca ctaatagaaa
    34981 tgtctggtgt tactaaaaaa taccgccgct caacgaccgc cctcagagac gtcaatgttt
    35041 ctgttaacca aggtgagttt gtttatctcg taggcccatc aggtgctggt aaatcaactt
    35101 ttatcaaact cctttaccgt gaagaacaac tgacaactgg taagctatat gtgggtgaat
    35161 tcaatttaac taaactaaaa gcacgtgatg ttccgattct tcgtcgtcac atcggagtag
    35221 tttttcaaga ttacaaactg ctaccaagaa aaacagtctt tgaaaatgta gcttacgcca
    35281 tggaagttat tggtgaaaag cgccgccaca ttaaaaaacg cgtgccagag gtactcgatt
    35341 tagttggctt gaaacataag atgcgttctt ttccaagtca gctatcagga ggggagcagc
    35401 aacgtgtggc cattgcgcgt gcgattgtta acaaccctaa attattgatt gcagatgaac
    35461 cgacagggaa tttagatcct gagatttcat gggaaattat gcagttactt gaacgtatta
    35521 atgttcaggg aacaactatc ttaatggcga cacacaacag tcatattgtg aatacattcc
    35581 gtcatcgtgt tgttgctatt gaagatggtc gcatagtacg tgatgaagaa aaaggagatt
    35641 atggttacga tgattagata ctttttccgt catatttggg aatccattaa aaatcttaaa
    35701 cgcaactttt ggatgacatt tgcttcagtt agtatggtgg ctgttacatt gactttagtt
    35761 ggtgtttttg cggcgacatt gttgaatata cagcgtgttg cttcgggtgt tgaaaataat
    35821 gtccatatca atacctactt acaagttgat tcaactgatg ctgcaaaagt tattcaaaat
    35881 actgccggtg aacctgttaa taacgataac taccacagtg tttacgacaa aattgctcag
    35941 ataaaaggtg tcaaaaagat tacattttct agcaaggatg aacaattgaa aaaattgcaa
    36001 gaaaccttag gtgatgtttg gaatatgtat gaccaagaca ctaatccgct ccaagatatc
    36061 tatcttattg agacccagac gcctaaacaa gttaaggcga tcactaaaaa gatcagaacg
    36121 attgaaggcg tagaagccgc agattatgga gggattaact ccgataaact atttaagttt
    36181 tcaacattaa ttcaaacttg ggggctgatc ggaacagcaa tgctattatt tgtagctgtc
    36241 tttctcattt caaacactat tcgtatgact attatgtctc gtaaacgcga cattgaaatt
    36301 atgcggttag ttggtgctaa aaattcttat attcgagggc ctttcttttt tgaaggggct
    36361 tgggttggct tgttaggagc tgttttacca tctcttctga tttattatgg atatgatctt
    36421 gtttataagc attttgcaca agaattacaa cgtaataatc tctcgatgta tccgcttgat
    36481 ccttatgtat attacctgat tggtgctttg tttgtcatag gtattatgat tggttcgctt
    36541 ggttcagtgc tttctatgag acgttattta aaattctaaa aaagcgctgc tgacgaaacg
    36601 tagcgaccct caaaagttgg acctcttatg ttattccaag gacttcgttt tgtattgcac
    36661 aggactggtc cttttcattt tgttttgatt catttgttta tttgtaatag gcaatatagt
    36721 ttaaaatagc agtttctaag gtttcaagag aagaaaagca ccccaaaagt tagatgtttg
    36781 atgtatctaa cttttggggt gtcattcaaa atttagtggc tcttttttag taaaatacta
    36841 tgatttcagt tttaggataa cctcaaacag tatctaaaat agctatgtca atgaaaaaag
    36901 gggttagcat ttttttcgtg acaaatggta gtagaaggac catgcccagg ataaactcgg
    36961 taatgattag gtaaggtgaa tagttcttgg cgaataccag taatgaggtc ctcaaagtta
    37021 ctggtaggta agtcagtacg accgatagtt tcacgaaata gagcatcgcc agtaacgact
    37081 aattcatctg agtggaaaac aaatgagact cctccccaag aatgaccagg tgttggtaaa
    37141 actgtgaatt caaaaccatt tagttgatag ggttgcttta atttaaaaaa gttctctgcc
    37201 ggtcttgcta taacagtaat gatatcatca tgacggccta agccagataa attatcatct
    37261 ggggaagata gccaagcggc ttctttttct gaaacataaa caggtggatg atcaaaagca
    37321 tctctgacca aatctaaact aaaaatatga tcataatgag tatgcgttaa gaggatagcc
    37381 actaaaggtt tttcaaagga tttaatttta gcaatgattt cttggccgtt acttcctgga
    37441 tcaatgagga taacagcttg atcattaacg aggtaataag tattttcacc agcaatgtgg
    37501 tttattgttt tatagatttt cataaggata gtgtaccaaa aaatcaaggc tttgttaaaa
    37561 aaagagtggc ggaaaataaa tggcaattag tttcagaaaa tatgagatct cacagcaatt
    37621 tcttgacacc gttttcataa aggtgtatca taaaagtaat caataagcca agcctaaagc
    37681 tgaacttagt ttaagaacac tgactttttg agagcaacct aatcggttga ctaaaaaata
    37741 tgaggtggaa gatatgtcaa aagttgctat tgttactggt gctggtcaag gaattggatt
    37801 tgctattgcg aaacgtcttc atgcagacgg ctttaaaata ggcattttag actataatga
    37861 agagactgct caggctgcgg ttgataaact atcacctgag gatactgttg cagttgtagc
    37921 agatgtttca aaacgtgacc aagtttttga tgctttccaa aaagttgtag atacttttgg
    37981 tgatttgaat gttgttgtta acaatgcagg tgttgcccca acaacccctc ttgacacgat
    38041 tactgaagag cagtttgaaa aggcttttgc aatcaatgtc ggaggaacga tttggggaag
    38101 ccaagcagct caaaaacatt ttagagaatt aggtcatggc ggaaaaatta taaatgcaac
    38161 atcacaagca ggatgtgaag ggaatcctaa tttgactgtt tatggtggta caaaatttgc
    38221 agttcgtggt attacacaga ctttggcaaa ggatttggca tcagaaggaa tcacagtcaa
    38281 tgcttacgca ccaggtattg ttaaaacacc tatgatgttt gatattgccc atgaagttgg
    38341 taaaaatgca ggcaaagatg atgaatgggg tatggagcaa tttgcaaaag atatcacttt
    38401 gaaacgcttg tctgaaccag aagatgttgc taatgcagta ggcttcttag cgggagatga
    38461 ttcaaattat atcactggac aaacaatagt tgtcgatggt ggtatggtat tccattaaaa
    38521 atgattaata ctgacactgt gagtttcact cacagtgttt tttaagaagt ataaaaaatt
    38581 atcctatctt ggttattgga gtgatggcac ctttgagata tagtcatgtt ttgttttata
    38641 gatatagcgt gctataatag acttactatg acccagaaaa aacttagaaa atatgcagta
    38701 gtggacttgg aagctactgg agcaggtccc aatgcttcta ttattcaggt tgggattgtt
    38761 attatccaag gaaataaaat tattgatagt tatgaaacag atgttaatcc tcatgaatct
    38821 ttagatgagc atattgttca tttgacaggt attacggata agcagttggc taaagctccg
    38881 gattttggtc aagtggcgca tcacatttat caattgattg aagattgtat ttttgttgcc
    38941 cacaatgtaa agttcgatgc aaatttattg gcagaagctc tttttttgga aggatatgag
    39001 ttaacaatac cacgtgttga tactgttgaa ttagcccaac tatttttccc tcgctttgaa
    39061 aagtataacc ttggtcacct atctaggcag ctcaatattg atttggcaga agctcatact
    39121 gctattgcag atgcaagagc tacagctata ctgtttttaa gacttttaca aaaaattgag
    39181 agccttccaa ttgagtgctt agaaagctta ttagtctatt cagatagcct gctatttgaa
    39241 acagcaatgg tcattcaaga aggattagcc aaagccaaac cttatgatcc caacaaatac
    39301 attaaaatca gacagattct cttaccaaag ggcccaaaag ctctcaagcc ttatcaaata
    39361 tcaaaaagct tccctattaa tatggcacta ttaggtctgg aagaaaggcc taaacagact
    39421 caatttgctc agttaattga cgaagattac catcagggag tagctagttt tattgaagct
    39481 cagacaggta ttggaaaaac ctacggctac ctgctacctc ttttggcaaa agaagatcaa
    39541 aaccaaatca ttgtcagtgt gccaaccaaa cttcttcaag accaattaat ggctggtgaa
    39601 gtagctgcta ttcaagaaca atttcacatc gcctgccata gtttaaaagg acccgccaat
    39661 tacctaaagc tagatagctt tgcagatagt ttggaccaaa atgaccagaa ccgtttggtg
    39721 aatcgttata aaatgcaatt attggtttgg ctcttagaaa ctaagacagg agaccttgac
    39781 gaaatcaaac aaaagcagcg ctttgccgct tattttgagc agcttaagca cgatggagac
    39841 attaagcagt cttctgaatt ttacaattat gatttttgga gagttaatta tgaaaaagcc
    39901 aaaacagccc gtctcctgat taccaaccat gcttattttt tacaccgtgt tcaagatgac
    39961 aaggactttg ctagaaacaa ggtcttggtc tttgatgagg cccaaaagct gatgctccaa
    40021 ttagatcaat tatcacgtca tcagcttaac ctgacggttt ttttacaaac aatacaggcc
    40081 aagttaagca accctttacc tcttttagaa aaacgcttat tggaaagctt gtcgtttgaa
    40141 ctgggacaag tgtcaagtga ttactatcaa aataaagagc accagcttgc tcatgattgg
    40201 tctcgtatag ctggctacgc caaagaatta acaggagcag attatcaaga gttacaagcc
    40261 ttttttgcta cctcagatgg tgactactgg cttagtagcg aaaagcaaga ggagaagcgg
    40321 ttgacttatc taaacagtgc tagtaaggca tttattcatt tccaacagct cttacccgaa
    40381 acggtgaaaa cttattttgt ttctgccact ttaaccatca gctcagaagt aaccttggca
    40441 gacctattag gttttgaaga gtatctctat catgtcattg aaaaggataa aaagcaagac
    40501 cagttggtgc ttgttgatca agaggcgcct atcgtgacag aagtcagtga ccaaatctat
    40561 gtggaagcaa ttgcaaaacg aatcgaaagc ttgaagcaag agggttatcc tattttagtg
    40621 ctttttaatt ctaaaaaaca cttgctgttg gtttcagact atctagacca atggcaggtg
    40681 cctcacttag cgcaggaaaa gaatggaaca gcttataata ttaaaaaacg gtttgatcaa
    40741 ggagagcaaa ctattttgct tggtttaggt tctttttggg aaggagttga ttttattcaa
    40801 gcggatcgta tgattaccct tatcgcacga ttgccctttg ataacccaga ggatttcttt
    40861 gttaagaaaa tgagtcatta tctattggaa aaaggtaaaa atcctttcag agattacttc
    40921 ttgccaatga ctattttgcg attaaaacag gctattggtc gcacgatgcg tcgacaagat
    40981 caaaagtcag tggttattat tttggatcgt cgtctcctta caaagtccta tggacaagtt
    41041 attttggaag gtctcggtca ggaatttttg atttctcaac aaaattttca cgattgtcta
    41101 gtagaaacgg actgtttttt gatataatag aaaggatcta ttatatcaga aaaattttta
    41161 atggcgttaa gcacaatcaa ctaacattta aattagtttg aacttttatt atgctctttt
    41221 agacaactgt tcacatgttt tcgaattgtt tttttgataa ggacaataaa tgaacaaatt
    41281 tttggtttta gtaaacccaa ttactgttct tttcattagt gtcagttgct atcaaaacag
    41341 cttgtcatag ttcgtgcgat cagtagcgag tattggtaaa actgaaaccg tagattatga
    41401 aaaaatgatc tgttgatcat taattaatct gaagataaga agtcgataaa aaggagatac
    41461 gatgcccaaa ctatcaaagc gagttttaga aatgaaagag agtgttactc tggctgcagg
    41521 tgctagagct aaggctttga aagcgcaggg ccgcgatgta ctgaatttaa ctttaggaga
    41581 accagatttt tttacaccta agcacattca agacaaggcg attgaatcta ttcaaaatgg
    41641 tacggctagc ttttatacaa atgcttctgg gcttccagaa ttaaaagctg cgattgccac
    41701 atacctgaaa aatcagtatg ggtatcactt gtctcctgat cagattgtgg caggaacagg
    41761 tgccaagttc atcctttatg ccttttttat ggctgtttta aatcctggtg accaagttct
    41821 cattccaaca ccatattggg tatcttattc tgaccaagta aaaatggcag aaggtcagcc
    41881 gatcttcgtt caaggactag aagaaaatca attcaaggta actgttgacc agctggaaag
    41941 agcaaggaca agtaagacta aagttgtcct tatcaattcc ccttccaatc caacaggtat
    42001 gatttatggt gctgaggaat taagggccat tggagaatgg gcggtgcaca acgatattct
    42061 tattctggca gatgatattt acgggtcctt agtctataac ggcaatcagt ttgttcctat
    42121 ttcaacactc tcagaggcga ttcgtcgtca aaccattacg gtcaatggcg tggctaaaag
    42181 ttatgccatg acaggctggc gggttggttt tgcagcaggt gaaccagaga tcatttcggc
    42241 aatgagtaag atcatcgggc agactacctc taacctgaca accgtttcac aatatgctgc
    42301 tatcgaggct ttctgtggta gtcagtcatc acttgaagag atgcgattag catttgaaga
    42361 acgccttaat atcacttatc cattgttgtg tcaggtccct gggtttgagg ttgttaaacc
    42421 acaaggagct ttttacttct ttccaaacgt taagaaagcc atggaaatga caggtttttc
    42481 cgatgtgaca agctttgcta atgctatttt agaggaagtc ggattagctg ttgtgagtgg
    42541 agcaggcttt ggcgcaccgg aaaatgttcg tctgagttat gctacagata tcgaaaccct
    42601 taaagaagct gttcgtagac tgcacgtctt tatggaaagt aatgaaatta attaaataaa
    42661 aatagagaga aacttatgtc aaaaaaactt atttcaattg ttgatgttaa agattatgtt
    42721 ggtcaagaag ttactattgg tgcttgggtt gctaacaaat caggtaaagg taagattgcg
    42781 tttgtacagt tacgcgatgg ttctgctttt ttccaaggtg tcgcttttaa accaaacttc
    42841 attgaaaaat atggtgaaga atcaggtctt gaaaaatttg acgttattaa acgtctcaac
    42901 caagaaacat ctgtttatgt aacagggatt gttaaagagg atgagcgttc taaatttggc
    42961 tatgagcttg atattactga ccttgaaatc attggagagt cacatgaata tcctataact
    43021 cctaaagagc atgggacaga cttcttgatg gacaaccgtc atctttggtt gcgttctcgt
    43081 aaacaaatgg ctgttatgca aattcgtaac gctattattt atgcaactta tgaattcttt
    43141 gaccaaaatg gttttatcaa atttgatagc ccaattttat cagaaaatgc agcagaagat
    43201 tcaacagagt tattcgaaac agactatttt gggaaaccag catttcttag ccaatctggt
    43261 caattatacc ttgaagctgg agcaatggct ctaggccgag ttttcgactt tggtccagtc
    43321 tttcgtgctg aaaaatctaa aacacgtcgc catttaacag aattttggat gatggatgct
    43381 gagtattctt tcttatcaca tgaggaatca cttgaccttc aagaagctta tgttaaagca
    43441 ttgatccaag gcgttctaga tcgtgcgcct caagcacttg atattttaga acgtgatgtt
    43501 gaggctctca aacgttatat cacagagcca ttcaagcgtg tttcttatga tgatgctatt
    43561 acacttcttc aagagcatga agcagatgaa gatacagatt atgaacattt agagcatggt
    43621 gatgactttg gttccccaca tgaaacttgg atttctaatt actttggagt accgacattt
    43681 gtagtgaact acccagcaag ctttaaagca ttttatatga aaccagttcc tggaaatcca
    43741 gaacgcgttc tttgtgctga tcttttagct ccagaaggtt atggagagat cattggcggc
    43801 tcaatgcgtg aagacaacta cgatgctctt gtggctaaaa tggatgaact aggtatggat
    43861 aaatcagaat atgactttta ccttgacctt cgtaaatatg gctctgttcc tcatggcggt
    43921 tttggtatag gtattgaacg tatggtaact tttgttgctg gtaccaaaca tattcgcgaa
    43981 gctattccat tcccacgtat gttacatcgg attagaccct aataatacaa aaaagttcat
    44041 gtcatctatg aactttttgt attggtaaga agtgagtgtg ttataaaaac atcaatatgt
    44101 cctaaaggag agataagaaa tagtagagtt tgaacctaca tcctttgtga agataaattt
    44161 tggaagttta gtagatcagt tagcagataa gacaatatag ccttgctcaa gggatatttt
    44221 atagacaagt atttggacta cgattatcaa atagacactc tcatttgata ctaaaaaggt
    44281 ctcgtctatt cctataatag gagagctacg ggagttatta ttaaaattag ctgattttta
    44341 cagttcaaaa tgtgttacca ctttttcact tcttagcttt cttatgtatg tggtataatg
    44401 aaggtggacg gttcgataga gaaagggaaa ttgtaaatgt cagacaaaca cattaattta
    44461 gttattgtga caggaatgag cggcgctgga aaaacagttg ccattcagtc ttttgaggat
    44521 ctaggctact ttaccattga taatatgccc ccagccttgg ttccaaaatt tttagaatta
    44581 attgaacaaa ccaatgaaaa tcgtagggtg gctttggttg tcgatatgag aagtcgtttg
    44641 tttttcaagg aaattaattc taccttagat agtattgaaa gcaatcctag cattgatttt
    44701 cggattcttt ttttggatgc aacggatgga gaattggtgt cacgctataa agaaaccaga
    44761 cggagccacc ctttggctgc ggacggtcgt gtgcttgatg gtattcgatt ggaaagagaa
    44821 ctcctatctc ctttgaaaag catgagccaa catgtggtgg atacaacaaa attgacccct
    44881 agacaattgc gtaaaaccat ttcagaccag ttttctgaag ggtctaatca agcctctttc
    44941 cgtattgaag tgatgagctt tgggttcaaa tatggtcttc ctttggatgc ggatttggtt
    45001 tttgatgtgc gttttctacc caatccttat tataaggtag agcttcgtga aaaaacagga
    45061 ctagatgagg acgtttttaa ttatgtgatg tctcacccag aatcagaggt gttttacaag
    45121 catttgttaa accttattgt ccctatctta ccggcttacc aaaaagaagg gaagtctgtc
    45181 ttgacggtgg ctatcggctg cacaggaggc caacaccgca gcgttgcctt tgcccattgc
    45241 ttggcagaaa gtctggcaac agattggtcg gttaatgaaa gccatcgtga tcaaaatcgt
    45301 cgtaaggaaa cggtgaatcg ttcatgaaaa atcctaaaat gactgttata ggtgggggta
    45361 ctggcatctc cattatccta aaaagccttc gtaatgaagc agttgatatt actgctgtgg
    45421 tcactgttgc agatgatggt ggttcctcag gagaacttcg taatgccatg caattagccc
    45481 cgccaggaga cttacggaat gtcttgttag cgatgagtga catgccaaaa ttttatgagc
    45541 gggttttcca ataccgtttt aacgagaatg atggagcctt ggctggtcac cctctaggca
    45601 atctcattat tgctggtatc tctgaaatgc aaggctcaac ctacaatgcc attcaaattt
    45661 taaccaaatt tttccatatt acaggtaaaa tttacccctc tagcgagcag gccttgaccc
    45721 ttcatgctgt ttttaaagat gggcatgaag tagcaggaga aagttctatt gccaaatacc
    45781 aaggcatgat tgaccatgtc tatgtgacca acacctacaa cgaccaaaag cctcaggcca
    45841 gtcgtaaggt ggttgaggct attctagaaa gtgacatgat tgtcttggga ccaggctcac
    45901 tgtttacctc tattttacct aatttggtca ttccagaaat taaagaagct ttgcgccaga
    45961 ctaaagcaga agtggtgtat atttgtaata tcatgaccca gtatggtgaa acagaacaat
    46021 tttcagatgc agaccatgtg gctgttttaa atcagcactt aggaagagat ttgattgata
    46081 cggttttggt aaatgtggct aaggtgccgc aggcttacat gaattctaac aaatttgatg
    46141 aatacctcgt tcaggttgat cacgactttg ctggcctttg cagggctgct aagcgtgtga
    46201 tttcgtctta ttttttacgg ttggagaatg gcggagcttt tcacgatggt aacttagtgg
    46261 tggaagaatt aatgaattta gtgaggatag tcaagcaatg agttttacaa ccaaggtcaa
    46321 agaggaatta attcacttgt ccacaggtga caacaatgaa ttggccgcca ttatcaaatt
    46381 atcaggcagc ttgggcttag ctcaccaaag ccttcattta tccattacta ctgaaaatgc
    46441 taaaattgcc cgctacattt attctcttat tgaagacgcc tatgttatcg tgccagaaat
    46501 caggtatcat caaaagacga acctgcgcaa aaaccgtgtt tacaccgtct atgttgagca
    46561 aggtgtggaa accattttag ccgatttgaa attagcggac tccttttttg gtctggaaac
    46621 aggcatcgaa cctcaggttt tatcagacga taatgctggc cgttcttatc ttaaaggggc
    46681 ttttctagcg gcaggtagca tcagagatcc tgagtcaggc aagtatcagt tagaaatcta
    46741 ttcagtttat cttgatcatg ctcaagactt agctcaactc atgcaaaaat ttatgctaga
    46801 tgccaaaacc attgagcata aaagcggtgc agttacttat ttgcaaaagg cagaagatat
    46861 catggacttt ttaattataa ttggtgccat gtcctgcaaa gaagactttg aagccatcaa
    46921 attacttcga gaagctcgaa atgatattaa ccgtgccaat aatgcagaaa cagctaatat
    46981 tgccaaaact atcagtgcta gcatgaaaac cattaataat attattaaaa tcatggacac
    47041 aattggttta gaaagtttac cgattgaatt gcaacaggta gcccagcttc gggttaagca
    47101 tccagactat tccattcaac aagttgctga tgcccttgag ttcccaatca ccaagagcgg
    47161 ggtcaaccat cgcttgcgta aaatcaataa aattgcagat gacttataga acatttttat
    47221 cgtatgagcc tttgttcata cgatttttct gtctaaaagt cgttttccat tgtttttttg
    47281 aagtgtctga tgttctgtga tatgataaaa gggataataa cgtttgtaaa ggagactgat
    47341 aatggcatgt acaactatac tcgtcggtaa aaaggcatct tatgatggct caaccatggt
    47401 tgctcgaaca gaagattctc aaaatggaga tttcacgcct aaaaaaatga ttgtggtgaa
    47461 accagaagat caaccgagac attaccgttc ggtgcaatct tcctttgaga tggacttacc
    47521 ggacaatccc atgacttaca cgtctgttcc cgatgcgctt ggtaaagatg gtatctgggc
    47581 agaagcaggc gtgaacgagg ctaatgtggc catgtcagca actgaaacca tcactaccaa
    47641 ttctcgtgtt ttaggggcag atcctctagt agcttctggt attggtgagg aggacatggt
    47701 gaccttagtg ctcccttata tccgttctgc gcgtgaaggg gttttgcgcc ttggtgccat
    47761 tttagaagac tatggcactt acgagtcaaa tggtgtggct ttttcagatg aacatgacat
    47821 ttggtggctg gaaacgattg gtggtcatca ctggattgca agacgggtgc cagatgatgc
    47881 ttatgtcacc aatcccaatc aatttggcat tgatcatttt gaatttaaca atcctgaaga
    47941 ttacctttgc tcagctgatt taaaggactt tattgacacg tatcacttag atttaaccta
    48001 tagctatgag cattttaatc cgcgctatgc ctttggtagt caacgtgaca aagaccgtca
    48061 ctataatacg ccacgtgctt ggatcatgca aaaattctta aacccagaaa ttgtgcaaga
    48121 cccgtgcagc tttgcccttg cttggtgtca aaaaccttac cgtaaaataa cagtagaaga
    48181 tgtcaaatat gtattgagta gtcactatca ggacacggtt tatgaccctt acggttcaga
    48241 aggaactcct gttagtaaga aagtctttcg ccctattggc attaaccgta ccagccaaac
    48301 cgctatctta cacattcgtc ctaataaacc tcaagaaatt gcggctatcc agtggatggc
    48361 ttatgggtca atgccattta ataccatggt gccttttttc acacaggtga agactattcc
    48421 agattatttt gccaatactt acgaaaatgt gtctaccgac aatttctatt ggaccaaccg
    48481 tctgattgcg gctctagcag atcctcacta caatcatcat gaaactgatt tggacaatta
    48541 cttggaagaa accatggcaa aaggtcatgc catgttacat gctgtggaag cacagttatt
    48601 ggctggtgaa accgtcgatt tggaagagga aaatcaaaaa atgagtgact atgtgcaagg
    48661 agaaacgcag acgctgttga acaagattct ctttgacgct agcaatctaa tgactaatcg
    48721 tttcagtttg agcgattaaa agtgaagcta aggctgagca actgcttagc cttttttacg
    48781 ataacttttt aggaaaagta atcgcaaaaa gacaaaaact tggaaaaaaa atcaaagtat
    48841 gctatactta actggtcaat ttttaaccag ttaataacaa aggaggaatc atgaaaaaga
    48901 aaattctttt aatgatgagt ttaatcagtg tcttttttgc ttggcaactt actcaggcaa
    48961 aacaagtctt agcagagggt aaagtgaagg tggtgacaac tttctatcct gtttatgaat
    49021 ttacaaaagg ggttattggt aatgatggcg atgtttccat gcttatgaaa gcaggaacgg
    49081 aacctcatga ttttgagcct tctacaaaag acattaaaaa aatccaagat gcagatgcat
    49141 ttgtttatat ggatgacaat atggaaactt gggtttctga tgtgaaaaaa tcattgacat
    49201 ctaaaaaagt gaccatcgtc aagggaactg gtaacatgct cttggtagca ggagctggac
    49261 atgaccatca ccatgaggat gctgacaaaa agcatgagcg taataaacat agcgaagaag
    49321 gacacaacca tgcttttgac ccacacgtgt ggttgtcacc ataccgtagc attacagtcg
    49381 ttgaaaatat tcgcgacagt ctttcaaaag cttacccaga aaaagcagag aacttcaaag
    49441 ccaatgccgc tacttatatt gaaaaattaa aagagcttga caaagactat acggcagcac
    49501 tttcagatgc taagcaaaag agctttgtga cacaacacgc agcttttggt tatatggcac
    49561 ttgactatgg cttgaaccaa atttctatta atggtgtcac accagatgca gaaccatcag
    49621 caaaacgtat tgctactttg tcaaaatacg ttaaaaaata tggcatcaaa tacatttatt
    49681 ttgaggaaaa tgcgtcaagt aaagtcgcaa aaaccctagc taaagaagca ggagttaaag
    49741 cggctgtgct tagtccgctt gaaggtttga ctgaaaaaga gatgaaagct ggccaagatt
    49801 actttacggt catgcgtaaa aaccttgaaa ccttacgctt aaccactgat gtggctggta
    49861 aagaaattct tccagaaaaa gacacgacta agacagttta caatggttat ttcaaagaca
    49921 aagaagtcaa agatcgtcaa ttatctgact ggtcaggtag ctggcaatct gtttacccct
    49981 atctacaaga tggtacttta gaccaagttt gggactacaa ggctaaaaaa tctaaaggta
    50041 aaatgacagc agccgagtac aaagattact acactactgg ttataaaact gacgtggaac
    50101 aaatcaaaat caatggtaag aaaaagacca tgacctttgt tcgtaatggt gaaaagaaaa
    50161 ccttcactta cacatacgcc ggcaaagaaa tcttgaccta tccaaaagga aatcgtggag
    50221 ttcgtttcat gtttgaagct aaagaaccaa atgctggcga attcaaatac gttcaattta
    50281 gtgaccatgc cattgctcct gaaaaagcag agcatttcca cctgtactgg ggtggtgaca
    50341 gccaagaaaa attacataaa gagttagaac attggccaac ttactatggt tcagacttat
    50401 ctggtcgtga aattgcccaa gaaatcaatg ctcattaatc ttaactcact aagcaaatct
    50461 tcttttaagg aggtttgctt tttctttgtg gagtaaaaaa attaataatt ctcaacatta
    50521 atttgacggg cacttatgat tataatatac tttaagtggt tataacaagt tagaggtgaa
    50581 taagatgcca aaagaacaac cactctattt acagattgtt gatgacttgg aagtgaaaat
    50641 caaaaaaagc atgacagaaa acgataagtt gttgtcagaa cgagaattaa gtgaccttta
    50701 cggtgttagt cgtatcacga ttagactggc tcttaaggaa ttggaattac gagacttaat
    50761 ttataaaaag cagggcaagg ggacttatgt ttcaggaatc aaagaacctg tcactgattt
    50821 atcaagtaca tatagcttta cagagcagat gaaaaaaatg ggaaaaatgc ctaaaacaga
    50881 aattctttcc tttgaacagt atcaggtaac accttacttg tctggtctct tagagcttga
    50941 tcccgataca gaagtttttg aattagaacg cctaaggatt gcagatgata tgcccttgat
    51001 gtttgaacga tcctatattc ctgctcagcc ttttcagggg ttaagcattg cggacttgaa
    51061 gcgtaaagct ttgtacgata tctttgcaaa ggactatcaa gagacaatcc gtcttgcaga
    51121 agaggagttt tatgccagca ttgccttgga ctatgaagcg gaccttttgg gaatcaaaaa
    51181 aggagatcct gtcttacata ttattcgtaa aacttataac gataaaaata ttttgatcga
    51241 atttactttc agtattgcaa gagcagacca gtttaggtat agggtacaac atcaccccaa
    51301 tggttaaaga aatgggaggt taatacatgt ttacaaaaac gcaggaagct ttggaggctc
    51361 ttggtgcagc tatcactacc aaggaaatta aacaagaacc ccgtttatgg caagaaacca
    51421 tgacgttttt tgaagaaaca agagattctc tggattcttt tttaaagaga gtttgtaaaa
    51481 gcgctaatgg aaataacgtt catgtcatct ttacaggtgc gggtacatct gagtacatcg
    51541 gtaataccat ttgcccttac ttgaagaagg tggggaatcg gcagtgctat ctctttgaga
    51601 gtgttgcttc aaccgatttg gttgcggcgc cagattatta tcttgctgag gaagagacgg
    51661 tactattagt ctcctttgct aggagtggta atagtccaga aagtgtagcg gctgttaacc
    51721 tggtcaatca actagtgcca aatagttatc atttaaccat tacgtgtgcc aaagatggag
    51781 aactggcgaa aaaagcacag caagatgagc gttcttacct ttatttgatg cctgaagacg
    51841 ctaatgatgc tggttttgcc atgactggta gttttacctg tatgatgcta gcggctcttt
    51901 tgatttttga tgatgctaac agtatagctc agaaagaagt ttatgtgact gatatgattc
    51961 ttgctgggca agctgttatt gaccaagaaa gacggctaca agagctagct gatttaggat
    52021 ttgaacgttt agtttatcta ggttctgctg gtttagccaa gttaacccaa gaagctcaat
    52081 taaaaatgtt agaattgaca gctggacaag ttgcgaccct ctacgagtcc tctatgggct
    52141 ttaggcatgg tccgaaatct tttattaatg accgtacctt agtcattgga tttgtcaata
    52201 atgatgctta cgtcagacaa tacgatttgg acatgttaga ggaaatccaa gcagatggca
    52261 ttgcccttaa aaccttggct ctattgcaag gaggggatat caacttttca gaggaccaat
    52321 ttcgactaga cacccgtcac ttattgccag aagcttactt agcttttccc atgattttag
    52381 tcgcgcagac cttggccctc ttaacggccg ttacattagg aaattcccct gacaccccat
    52441 ctgcgacagg aacggttaat cgtgtggtca aaggagtaac gattcaccct tacccgacta
    52501 gtagtcgtca atgcaacaaa gcttaaagct tttgtgtaga agccgtaaaa cgaaatgatt
    52561 ttgtgctagt aggccaaaag aaaaaccctc ctgatacagg aaggtttttt gtattgtctt
    52621 gcttattttg ctgcgtttgc gtctttgaga ccgtattttt tgttgaagcg gtccacacgt
    52681 ccatctgctt gagtgaactt ttgacgtcct gtgtagaatg ggtgtgaatc tgatgaaatt
    52741 tccacacgga taagtgggta agtttcacct tcaaactcaa cagtttcttt agaagctttt
    52801 gttgagccgc taaggaactg gtaacctgta gttgtatcaa ggaaaacaac tggacgataa
    52861 tctggatgaa tgtcttttct catcttaaaa aattcctttc tgccatgaca cttgatgcgt
    52921 catagttatt aactattcta gtgtaacaaa acttgaatga cttgacaagg cttttctttc
    52981 cttatttttt aaaaatccta aatctcttgg caaacagcga tgagttctcg gaaaatagct
    53041 tgattttctt ctaaattagc tgagttggct ccgctagcaa ggggatgccc ccctccaccg
    53101 tgacgcttag caatgccatt aataatcttt tctttactgc gcatacgcac acggtagttg
    53161 ccgtcagtta actcaacaaa aatagcccaa gcttgaacgt tatcaatttt accaggagca
    53221 cagacaatgg cagagctttc tgctagggtc acgtcaaaat gttttaaggt ttcttggctg
    53281 acaaggacat aagcagcccc actctcatca attgttaaat gctcaaagac gtagctttgc
    53341 agctttgcta ttttcaaagg aaacgaatcc atttgccttg aaatcgcagc aaagtcgaac
    53401 tcgaaatgtc tgagttggct agcaatggaa agggttttac tagtggttga ggcataaaga
    53461 aatcgccctg tatcaccaac gataccagtg tataagagct tagcagcctt gtcagagaga
    53521 gtaagattct gactaaaggc aaagtctgca atgatttcac ttgcgctaga agcgcttgtg
    53581 tccacataat agaagtcacc atacacatca tcgttgggat ggtgatcaat cttaattaag
    53641 cactgcccca gtgtgtagcg ctcatcatca atccttggtc gattcgctgt atctgtaatg
    53701 atgaccaaag cctctttata gtctttgtca gtcacctgat ccatttggct aatccaagct
    53761 aaactaggct catcaaaacc agtcattaaa acctttttgt ctgggaaatt ttgtgcaata
    53821 atttctttca agccggcctg actaccaaga gcatcagggt caggattttg atggcgatgg
    53881 ataataatag tttggtgggc ttttatttta tctaaaattg tttcaaaagt tgttatcatg
    53941 ctttaatctt tctttttcaa aaagaggaaa ataaccgttt aacatagtct tcaaaaaacg
    54001 gtttgtgtca taaggaggtg tgcgttatgt taagtggcta atgctagaat tttttaagat
    54061 tgtcaaagac cataaattga cattttatct gaaaatcata tcctatttta ttatagcata
    54121 aaagtaagct ctagataatg gtaaattaat ctaggaagaa aagaggttag ttatcagtta
    54181 tgatactatc aaagctagtc ttgcaaatgc aagtgaaaaa atagttttta agtgttttaa
    54241 gtaatcatat caaccttctc gttaaacaat tagtgctttt cctaaaaaag tgtgttaaaa
    54301 taaacatagt ttagtaattt tgggaggaat ttatggcatt agcaaaaatc gtttatgcca
    54361 gcatgacggg aaatactgaa gaaatcgctg atatcgttgc aaataaattg caagaattgg
    54421 gccatgatgt tgacattgat gagtgcacaa cggttgacgc ttcagagttt gagaatgctg
    54481 atatcgctgt cgttgcgact tatacttatg gtgacggtga cttaccagat gaaatcgttg
    54541 atttttacga agatttacaa gatttagatt tagaaggtaa aatctatggc gttgtcggct
    54601 ctggtgacac tttttatgac tatttttgca aatctgttga tgacttttca gagcaatttg
    54661 cgcttactgg tgccatcaaa ggtgcagagc cagtcaaagt agatttggca gcagaagatg
    54721 aagacatcga tcgtttagag gcctttgcag agcaactctc acaggctgtt aataatctat
    54781 aaaactattt agtaaaaatg tcagggactt ccttggcatt tttagatttt taacctaaaa
    54841 taaagggaat gtcacttcag ggtaggaatt gaaaaaaaca atcagcttca ccttgcttct
    54901 gatcctctta ttaactataa taaaaagata atctaataca tctagaaaag gaaagttatg
    54961 cgtttagaag agatacgaca agaaatcaat agtattgatc accacttggt tgccttatta
    55021 gaaaagcgga tggcacttgt agagcaagta acagcttata aactagccaa tcacttgcct
    55081 gtcttagatc aggcgaggga aaatcagatc ctagataggg tttcttatct cgttaaagac
    55141 caagcatttg agccagctat ccatgagacg tttaaaacca ttatgtctct ttcacggcag
    55201 tatcaaacgc aacgcttaac aggtggtgac actaatgatt aaatcaattg caacactgcc
    55261 taaaccctcc ttaaaagaag cgttaggact aataggcttg gcgcttccca taggggtcgc
    55321 ggttggagtg gttgatgtga tttttggtaa aggacttttg tttctttccg agtatcgcga
    55381 tcaccacctt ttcttgcttc ttccttttct tgccctagca ggtcttgtta tcgtttttct
    55441 ttacgataag ttaggcaaag aggttcgtca agggatggga ttggtttttc aggtagggca
    55501 tggtcaaaaa gatcagattc ctcctattct cattcctctt attttgttct cgacttgggt
    55561 aacccactta tttggagcga gtgcgggtag agaaggggta gccgttcaaa taggagctac
    55621 catctctcat tattgtcaga gatttgtgac tagtcaagaa gccgcacgac accttttgat
    55681 tatggggatg gcagccggtt ttgcaggact ttttcaaacg ccgattgctg ctgtcgtatt
    55741 tgccctcgag gttttattgg taggaacgct tcgctactgc gctttattac cttccttagt
    55801 tgctgcttgc gtggcttctt ggacctctca ttctttggga ttagagaaat ttacgattgt
    55861 tcttgaagaa acattgacca taactccctt aaccttagtt aaattaattg ggttaggtct
    55921 tatttttggt ctggtgggaa atagttttgc ctgtttactt ggctggttta aaccttacct
    55981 gagtcaaaag ctaccaaatc cttatctcag gattgctttc attggtgttt tgttatctat
    56041 ctgtttgatg attggtcacg ttggaagata tagtggtcta gggactcatt tgcttgcggc
    56101 agctttttct ggccaaacta tcctaactta cgactggctt ttaaaaatga tcgtgacggt
    56161 gattagctta tctgcaggct ttcaaggtgg tgaagtgacg cctctatttg ctattggagc
    56221 ttctttaggt atcgttttgg ccccttactt aggtcttcct gtactgttgg ttgctgcact
    56281 tggttacacc acggtatttg gaagtgcaac aaataccttt tgggccccta tctttattgg
    56341 gatagaggtc tttggtccag agaatgccct agcatatttt gtcacctcgg ctgcagccta
    56401 tatggttagt cacagacatt ccatttacag ttatcaaaaa gtagtcatga tcaatttaaa
    56461 agacatgacg gtaaatcaga tggatttaaa aaaatagctt tacaataaag aagctttttg
    56521 ttagaataaa tctcgtgtgt aatggacaca cgaaaattac ggctaatccg ctaagacaag
    56581 tacttaagat tagtaagata ggagaataaa aatgaatcca ttaatccaaa gtttgacaga
    56641 aggtcaactt cgctctgata tccctaactt ccgtcctggt gacactgttc gcgttcacgc
    56701 aaaagttgtc gaaggaactc gcgaacgtat ccagatcttt gaaggtgttg ttatttcacg
    56761 taaaggtcaa gggatctcag aaatgtacac tgttcgtaaa atctcaggtg gtatcggggt
    56821 agagcgtaca ttcccaatcc acacaccacg tgttgacaaa atcgaagtta tccgccacgg
    56881 taaagtccgt cgtgcgaaac tttactactt gcgtgcatta caaggtaaag cagctcgtat
    56941 caaagaaatt cgtcgttagt ttgaaccgaa acgtaacgta atagaataaa aagactagag
    57001 tctctagtct ttttatgtcg tcgtgacaat gtgacacttg gtgattaacg ctatgctcgt
    57061 aggacacttt gaaagttcct tagcagttat aattagtata ttgtaataac aaaatgaagc
    57121 aaaataaact ttcagttatc tgatatttca tcttgcatta ccttattttc taggttaaaa
    57181 tagaagacaa ggtgaatata agataggccc cttagttcaa tggatataac aactccctcc
    57241 taaggagtag ttgctggttc gattccggca ggggtcatta ttgtcactga aaaagccttt
    57301 acatcaaagg ctttttgttt tattccggtt ctaaaagggg cagtttgttt atttataatt
    57361 tctttcatgt ttacagttgt gtgactgtaa atagataatg ttgtttttgg atcgctgtga
    57421 ccaactctat ccattatcgc atttagcggt atcccttttt ctgctaaaaa tgatatatgc
    57481 gagtgtctaa ataagtgcgt gtgataatct ccgtaaattt tcaatcgctt attgatgtac
    57541 gcgtttaaaa ttggtaaacc ttttgaatat ggaaagctgt tagcactctg cttgtgatta
    57601 atctatcgta tgttaaaagt gacatttaaa ttaaatcatt ttttatacat atatttcaac
    57661 aggatttatc atattggaaa atagcggtgc ttctattggc attttactat tgacaggaat
    57721 ttccctgcct tttgtctcct aaagaagatc agctttgatt tctaacttga ttagagtggg
    57781 cttggtactg tttatgaatt atcagtgcat actttctgga gatcaaaaag gaaaggagca
    57841 gctaaagagg tgtatgtgtt accattagag gaatagagca tcgcaagact cgtttattca
    57901 gtcattacga tacctgtttt atttgggaaa taactataat attaagtgaa tttgtgatat
    57961 aatgaaaggt atgaattatc aagattatat atgggatttg ggaggtacct tactcgataa
    58021 ttacgagctt tcaacccaag cttttgtcca aacgctagct tttttcagcc ttccaggaga
    58081 tcacgatgct gtttaccaaa aattaaaaga atcaaccgct atcgctgtgg caacgtttgc
    58141 tcctaatgag ccagaatttt tgcatgtgta taggctaaga gaagcagaca aattggcaca
    58201 acccatttgg tgtttgggag ctaaagaaat tttggaaaag atagcgactt caggttcgcg
    58261 aaatttttta atttctcatc gagattgtca ggtgaaccaa cttttagagc aagctgggtt
    58321 gttaatctat tttacagaag tcgtgacagc ttcaaatggc tttgctcgga aaccaaatcc
    58381 tgaaagtttg ttttatttaa aagaaaagta tgatattaat agtggattag taattggaga
    58441 tcggctaatt gataaacaag caggacaagc agcgggtttt aataccttgc ttgttgacgg
    58501 tagaaaaaac ctattggaga tagtaacgta gatgattgaa gaaaataaac attttgaaaa
    58561 aaaaatgcaa gaatacgatg ccagtcaaat tcaggttcta gaagggctgg aggctgtccg
    58621 aatgcgtcca gggatgtata ttggctcgac agctaaagag ggtttgcatc atttagtctg
    58681 ggaaattgtt gacaactcaa ttgacgaagc attagcaggt tttgcctctc atattaaagt
    58741 ctttattgaa gcagataatt ccattacagt agtagacgat ggccgtggaa ttccagttga
    58801 catccaagcc aagacaggac gtcccgccgt tgaaacagtt tttacagtct tacacgcagg
    58861 tggtaaattt ggcggaggcg gctataaggt ttctggagga ctacatggtg tagggtcatc
    58921 tgttgttaat gctttatcaa cacaattaga tgtgcgtgtt tataaaaacg gccaaattca
    58981 ttaccaaaaa tttaaacgcg gagctgttgt agcagatctt gagatcattg gaaccactga
    59041 tgtgactgga acaacggtac actttacacc cgatccagaa atttttactg aaacgactca
    59101 gtttgattac agtgttttag caaaacgtat tcaagagtta gcctttttga atcgtggttt
    59161 aaaaatttcc attacagata agcgctcagg tatggaacaa gaagaacatt tccattatga
    59221 aggtggaatt ggttcttatg ttgaattttt aaatgataaa aaagatgtta tctttgaaac
    59281 gcctatctat acagatggtg aattagaagg tattgcagtt gaagtagcca tgcaatacac
    59341 gactagctat caagaaacag tcatgagttt tgctaataat attcatactc atgaaggtgg
    59401 aacgcatgaa caaggcttta gagcggctct tactcgggtc atcaatgact acgctaagaa
    59461 aaataaaatt cttaaagaaa atgaggacaa tttgacagga gaagatgttc gtgaaggttt
    59521 gacggcggta atttctgtta agcatccaaa tcctcaattt gaaggtcaaa ccaaaacaaa
    59581 attgggcaac tcagaagtgg ttaagatcac taatcgtctc tttagtgagg cctttcaacg
    59641 ttttcttttg gaaaacccac aagttgctcg taagattgtg gaaaaaggga ttttggcttc
    59701 taaagctaga attgcagcta agcgagcccg cgaagtcacc cgcaaaaaat caggcttaga
    59761 aatttcaaac ttacctggaa aattagcaga ctgttcgtca aatgatgcta accaaaacga
    59821 acttttcatc gtcgaaggag attcagcggg tgggtcggcc aaatcaggtc gtaaccgaga
    59881 gtttcaagct atcttgccta ttcgtggtaa aattttgaac gtggaaaaag caactatgga
    59941 taagattctt gccaacgaag aaattagaag tctttttacc gctatgggta caggttttgg
    60001 tgcagatttt gacgtgtcaa aagctcgcta ccaaaagctg gttatcatga ccgatgccga
    60061 tgtggatggc gctcatatta gaaccttgct tttaaccttg atttaccgct ttatgaggcc
    60121 tgttctagaa gctggctatg tttatatcgc ccagccacct atttatggtg ttaaggtcgg
    60181 tagtgagatt aaagagtata ttcagccagg tattgatcaa gaagaccaat taaaaacagc
    60241 tcttgaaaaa tatagtattg gtcgttcaaa accaactgtt caacgttata aaggtcttgg
    60301 ggaaatggat gaccatcaac tttgggaaac tactatggat cctgaaaatc gtttgatggc
    60361 gcgtgtgaca gttgatgatg ccgcagaagc agataaagta tttgatatgt taatgggaga
    60421 tcgtgttgaa ccaagacgtg atttcattga ggaaaatgcg gtttatagta cactggatat
    60481 ttagtcacct ttttagtttc acaaggaatc tgctaccaaa gaggacgaat tgactaaaaa
    60541 tcatgatgca tgatgccaac agctttgtta gttagtacgt cttcatcttg agggacagcg
    60601 ttaatgcata ggtattgaga ccgcccctta atacctatgt tagtccttat taagaaagcc
    60661 ttttgagtcg ctggtacgag attgttgaat tagtcaaata tattttgaaa tgcattttaa
    60721 agtatgttat aatcgtaatg ttatagtgta ttttaaataa aattttaata aaaggcgatt
    60781 gaggaatttc agtttttagt tattactgga acaatgttga taaatcgtgt ctaaataata
    60841 aggagattca gatgtcaagc ggaattatct tgctgattgt ggctatagtc ctactagtga
    60901 ttatcgccta tctagtgggt gtcatcatac gaaagcgtaa tgattcccta attaccagtc
    60961 tagaagaacg aaaacaggcc ttgtttgctt tgcctgttaa tgatgaaatt gaagaagtaa
    61021 agtcacttca tttgatagga cagagccaaa cctcttttcg ggaatggaat cagaaatggg
    61081 tagacttaac tgtaaattcg tttgcagata ttgaaaatca tatttttgaa gctgaaaatt
    61141 tgaatgatac gtttaatttt attcgcgcca agcatgagat taatagcgtt gaaagtcagc
    61201 ttaatcttgt tgaagaagat atcgcttcta ttcgtgaggc acttaatatc ttaaaagagc
    61261 aagaagaaaa aaatagtgct cgtgtcaccc atgctcttga tttatatgaa aagctccaag
    61321 cttctatttc tgagaatgaa gataattttg gttcaactat gcctgaaatt gacaaacaga
    61381 tgaaaaacat tgaaactgaa ttttcacagt ttgtcgcact caactcatca ggtgatcctg
    61441 tagaagcttc tgaagttctg gatagagcag aagagcatac tattgctcta gggcagatta
    61501 cagagcagat tccagctatt gtggccaagt tggaagatga ttttccagat cagttagatg
    61561 atttggaaac aggatatcgc cgtttacttg aagaaaacta tcattttcca gaaaaaaaca
    61621 ttgaggcacg ttttcaggag attcgtgagt ctattcgtgc caattcatca gagttagtta
    61681 cacttgactt ggatcgtgct agagaagaaa acacgcatat tcaagaacgc attgattctc
    61741 tttatgaagt ctttgagcgc gaaattgccg cttataaggt agctgctaaa aatagtaaga
    61801 tgctacctcg ttacctagca catgtgaaac gtaataatga acaactcaaa gatgaaattg
    61861 cacgtctatc acgtaaatat attctaagtg aaacggaaag tctgactgtt aaggcatttg
    61921 agaaggatat taaagagatc gaagacagta cacttgctgt tgcagaacaa tttggtttac
    61981 aagaaaaacc attttcagaa ctgcaagtga cttttgaacg cagtataaag acgcttacta
    62041 atgtcgagtc aggtcaaatg gatgtttttg cagcagttaa agacattgaa aaaattgaat
    62101 cacaggcacg ccataacctt gatgtttatg ttactcagct tcatatgatt aaacgttata
    62161 tggaaaaacg tcacttgcct ggaattccac aagactttct tagtgctttc tttacaacga
    62221 gttcacagtt ggaagctttg atggatgagc ttagtagagg acggatcaat attgaggcag
    62281 tgtcacgttt gttggaagtt gcgacggttg ctattgctaa tctagaagat ttaacctatc
    62341 aagtggttca aaatgctacc ttgactgaac aattactaca atattctaat cgctatcgtt
    62401 cttttgaagc aggagttcaa agtagttttg aacatgcttt gagacttttc gaagtagaaa
    62461 atgattatca ggcttctttt gatgaaattt cttatgctct tgaaacagtt gaacccgggg
    62521 taacagatcg gtttgttaac tcttatgaaa aaacacgaga gcacattcgt ttttgatact
    62581 tgaaagtcgt gatattctca cgacttttta ttagcttttt ggaccaatac gtgtttttga
    62641 ctagaatctt ctttttaatg atcaaaagaa gaagtagtaa cagtgttttt tgctattgag
    62701 aggatagtta ttttttcaaa aatcttttcc aaaaggattt ggttttttca ggagtttttt
    62761 tgctaagttg atcgtttttt gtctgcttta agatgacttc taaacgtgtt tcctttagat
    62821 ttattgcgtg gtcggtatga aggataaacg caaaaggtga agtcatatgc gtttctgtaa
    62881 taatagtaac aggtaaatgg ttttcttttg ctaatttcat atagagtatt tgaagttgag
    62941 gtgccagctt ggaagagatt tttaagtgta aaggttgata gactaactga aggctcggta
    63001 attctctttt caaatagtct tttactgttt tagtttgagc ttcgtctaat aaagcactta
    63061 aaacgactcg ttcagcatac gttcctagat agtagcgttg ctgctcagga tttaatcttc
    63121 tttctcccca ggctcctttt aatagcttat cttctaaatt agtcatagat accctcgctt
    63181 ctttataata gtttctttat tatatcgatt tctacaataa aataccaatt tgaaaatttt
    63241 cggtaaatgt gttaaaaaac aagaaagaaa gtgatttttc ttgcaaaaaa caaaggaagc
    63301 ttttcatgag attgcttttt aagttgatta aaggcctagt ttgtggtatt atgaacatgt
    63361 aaaatattaa aactcataag gagagtatta caatgtcaat tattactgat gtatacgctc
    63421 gcgaagtcct tgactcacgc ggtaacccaa cacttgaagt agaagtttat acagaatcag
    63481 gtgcattcgg acgtggtatg gttccttcag gagcttcaac tggtgaacat gaagccgttg
    63541 aacttcgtga tggtgacaaa tctcgttacc ttggtctagg tactcaaaaa gcagttgata
    63601 acgttaataa cattatcgct gaggcaatca tcggttacga tgttcgcgat caacaagcta
    63661 tcgaccgtgc aatgatcgca cttgacggta ctcctaacaa aggtaaactt ggtgctaacg
    63721 ctgttcttgg tgtttctatc gctgttgctc gtgctgctgc tgactacctt gaagtgccac
    63781 tttacactta ccttggtgga ttcaacacta aagttcttcc aactcctatg atgaacatca
    63841 tcaacggtgg atcacactca gatgctccaa ttgcattcca agagttcatg atcatgccag
    63901 ttggtgcacc tactttcaaa gaaggtcttc gttggggtgc tgaagttttc cacgctctta
    63961 agaaaatcct taaagaacgc ggacttgtta cagctgtagg tgacgaaggt ggatttgctc
    64021 ctaaatttga aggaactgaa gacggtgtag aaactatcct taaagctatt gaagcagctg
    64081 gttacgaagc tggtgaaaac ggcattatga tcggtttcga ctgtgcatca tcagaattct
    64141 acgacaaaga gcgtggcgtt tacgactaca ctaaatttga aggtgaaggc gctgctgttc
    64201 gtacatctgc agaacaaatc gactaccttg aagagttggt taacaaatac ccaattatta
    64261 ctatcgaaga tggtatggac gaaaacgact gggatggttg gaaagctctt actgaacgcc
    64321 taggcggacg tgttcaattg gttggtgacg acttcttcgt tacaaatact gaataccttg
    64381 ctcgtggtat caaagaaaat gcagctaact caatccttat caaagttaac caaatcggta
    64441 ctttgactga aacttttgaa gctatcgaaa tggctaaaga agctggttac actgccgttg
    64501 tatcacaccg ttcaggtgaa actgaagatt caacaatcgc tgacatcgca gttgcaacaa
    64561 acgctggcca aatcaaaaca ggttcattgt cacgtacaga ccgtattgct aaatacaacc
    64621 aattgcttcg tatcgaagat caacttggtg aagttgctca atacaaagga atcaaatcat
    64681 tctataactt aaaaaaatag acggtaagac agaaatcggt cattcgtaga atgcgatttc
    64741 gtcgtcgcac ctccgtcagg ctgactaggt tggtcacgac aaaaggagtg gacatccagt
    64801 cagctacgac gcaaaagtta aagattaaag aatagaaagc ttgggaaaat cccaagcttt
    64861 ttgatttcta aatagtgaaa atttactgtg gactaattta tcctgagcta atgtattagc
    64921 gaggtaaagt tattgaggaa ggcttggatt tgtactgccc ccaaacagtt ggacactata
    64981 ttttaaatac agaactaagt ccttttatac caattgtcaa gcacatcaag gttttaactg
    65041 aaaaaatgtt ttagacaata gcctaggttt agctgttgtc ttacttgttg tgctctgatt
    65101 agcataaaaa agtgtacaag aaaaaaacct tgcctagtat ttttaagatc aaggtgttac
    65161 aatgatagag caacaacgac tttaatgatt ttgggtcgaa gcgtaattgt aaagtttgtt
    65221 atgcgttatg aggtaaagca ttgtcctgac aaggcaatgt ctagaggcaa tcgtatgtgg
    65281 cttggttgaa gctgtttgcg attgcctttt tcgtttctca tagaagtctg cgatatgaca
    65341 aggattggta tgactagcag aagcaatgtc atggatacac ttgaaaagaa tctttggagc
    65401 ataaggattc caacgtttgg taatgtgctc ttgagctaga tagttgccag actcataatg
    65461 tctaaagtca atcccaatga aagcattaat ttgattagca gattgaaaac gacgaatagc
    65521 accgagttcg ccaataatac ttgttgcagt tgtttctgcg atacttggaa tagagcgtag
    65581 aatcttgtct tctggtaaag gttgagccaa cgccaccatc ttatcaaaaa tagcttgacg
    65641 acgttcagaa agtctaagta attctttggc atagcaacga acctcttcta tcatcggaga
    65701 ggttttcgta acagtaaaat aagactgctt ggctaatgag acaagttttt ccactaagta
    65761 gatcccacac ttgtcagaga tacgtttaga ggtagattga cggatgatag ctgacaatgc
    65821 tttgtcagct agctctagta caaagctatt acatggaaaa gccgttacca cattccagta
    65881 ttactcacca gttggtgcaa ataagatgtg ttccagttca ggaaaagtga cttgtaagat
    65941 cttgtgaagg cgattcttaa ctctcgaaca atattatcct cagtcaattt ttgatagaag
    66001 cgactcaagt cacgtagcag ctgataaact tcttcctgaa cgtaagttgg ttttctaact
    66061 agaataaatt gagactgagc caatttttca gtgtctaact tatccgcttt acgcacccgt
    66121 agactatcta gttttctaaa ggattgagtt gtgtataagc gtagccattt ttctcaagga
    66181 aggactgaag ccgacgagaa tagactcctg ttgtgatggt ctcccttttg ccttttaaac
    66241 aatagtatag ccgtttttca agatgttatt aatgattttt tcctttattc tagaagaata
    66301 agtctgatac tgtttcttct taataatctc aatgccctaa cagtccatca gtcgaacggt
    66361 atttctcaaa ttactgcagt tgactcttgc gttcagttcc ctcaccttgt ggatgccgtt
    66421 tagaacaata ttagcttgct gagccttttg gtaatgtgaa caaagcatct ttatcactca
    66481 aggagaagac aggttcttgt ggtaagtgtc caacaaggtc acgataaact catcgtaccg
    66541 tttttggcgg aagtctagaa atgccttatg aatgtaagcc tcagaaacgt ctctaaaact
    66601 aaacaagaaa tacaagaaca aatgcccttg attctggtac aaatgctgta tcaggttcag
    66661 gtattgccta tctgcgataa tgacactctg cttttcctta acaatatctt tgaaaggaat
    66721 gatttcgaca ttgacaggca taaccgttgc aatagctggc gggaagtaaa tcgagagtgc
    66781 attggtcatc aaatccagcg cagcagtgtt attggtcaaa agatagacag gtatcggtgg
    66841 caaatatgtt tttaggagtt cctctaactg tatcgtgaag agcagtaaat gataggacgg
    66901 cagtttcccc acgatgcctt ctgcgctcat ccattcctca acgatggttt ttagagtatt
    66961 gtagaagctc ttgtttcgtt tttgctgtct tctgaggtaa tggaacaagt gtatgtcagg
    67021 taacaaaact tgaagattac tgataaagag acgtgagaag gaaaccaaaa tccgtttgaa
    67081 aggaagggat tttaggatat tattgcctag tagtggacgc agggatgctt tcaaggtttt
    67141 tccaattgtt tttgtcatca ccaactcaaa caattctttc acttttttag gtgtccattt
    67201 atccttatgg aaaggattat tggtggtgca aaaacataag aaaatataat ccaaatcctc
    67261 atcagttaaa tcaggatggt accattttcc gatgatttcc tgactggtca ttttgatatc
    67321 gtgatagata gacagttttt tcaagtgttt gaatgcatct gtctgaggga tccccaagtt
    67381 atcatgtcgc cgtttccata taagggatat cagaatggca aagtacgaaa attcataagg
    67441 ggctttactg gcatcattaa gtgtgatggc ttgattggag tttataatca gcgaaaccac
    67501 tttattcatt gatgttttat caaaatcata aatggtcata ccaaactgat agtgcaacaa
    67561 ggcgatcaga aagcggatac ggtattttgg tccaacaaca tggttttgcc gaacgtttaa
    67621 acctaccttt ttcaagtatt tccgacaatt ggtcctgata cggtaagcgg tggcttggga
    67681 aatatactct cgtttaatga agtcgtgtag gctcagctct cctggttcaa ttaaaaaaca
    67741 taatatcttt aagaatatcg aggtttgata gagtttctta aaataaggca ttttaagttg
    67801 gtaggtctta taggggatag aaacagtgtc gtcacaaatg gttaaggtga gggtgtcttc
    67861 taaatcctta atcatttcaa agagtaagtt gtagacacgt tgttttttga gttctgtttc
    67921 ctgacacaac tggtctatgg agacttcgga aacgtctaac aaaatgttga gcagtgataa
    67981 tttatcttgt aatttggttt ctagatgaag gtgtaacata actgaactcc tttcattttt
    68041 atagcagaaa ctcgtattta tttaattatc acataattaa ataaaacagt caaataattc
    68101 aatggtgacg cgtgtttaag gagtattgag aatgtcagat ataagcaaaa tgcagctgaa
    68161 tattcttaat tttaggtatg aaaaagcgtc gggattgata aaaaatctat tgaattgggt
    68221 aaatttaagt tgatataata agttgattag aaaaattaat aattcattta aattctaatc
    68281 agctgtaacg ttccggggca tgttaaactg tctgaatata gaaaatacag gtttctaagg
    68341 tgactaagat aagttaagga ggattcctta aattctgtga taggaacgta aactctctac
    68401 aagcgtagag cacgtcatca gtcagtctgg gtgtctactg gctgacaacg tcaaataaga
    68461 taggcatgaa aaaataaggg ggaacacaga atgtttttaa aacaccaaga tgtgaaacaa
    68521 aaaaattgga gaatgcgtaa ggtcaaaaaa ctctttgtta gttcatgtat gcttttaaca
    68581 gtcggcctcg gagttgccgt acctactgga ttcagccaat ctaatggcgt gatggttgta
    68641 aaggctgatg ttacacaaga aggaagtcct gaaaatgttt tgaagatagc taattcttta
    68701 aaggctacaa ctgatggtac aaaaataggt agatatagtg caataaatac tcaaaatcta
    68761 gatgtttctg aatataaatt ttattatgtt aactcaacta aattaaataa aaatcctcaa
    68821 caacgcttat ggcactatga aaaaccaaaa gaaatatatg attctaccat tcgagttgcg
    68881 ggtgatggaa ctgctaatat tcgtttttat gcagatggag atggtagtaa gactcctaat
    68941 ggattctctt ttgcatataa gttcaatact actaatctaa aaacagacca caaatataag
    69001 ttgtcgttca agcaaactca aaataatgac gaagaggttt atacaaagta tctggtaggt
    69061 aaagcaagtg aaggtgtcgc taattattta acatctgaag aaacttcggt agctcggaat
    69121 gttgagttat ttgaaggtaa tcaaaagacg actgcagaga gaaaatttgc aaaacatcaa
    69181 tcaggagaga aatctcttca gtttatttca aaaacagatg gaactgctag tttagtttta
    69241 gcagctggtg taaaaaatac tcccaagaaa aatacagata taacctttga cagttttgaa
    69301 tttattgata taaccgaacc agctcaagtt agttctggag aagcgattgc aggaaataaa
    69361 aatcttacgg taaatctaag tggttcagac ggtagaaaaa acttcagcgg ggaaatcatt
    69421 gaagtttata agaatggaca attattaaaa aaagaaacag taactacggc gggtaataaa
    69481 gttaatattg ctttgagtga tgatgttgtt ttgaaaaaag atgatgaaat tacctttaaa
    69541 gtaaagcaag gtagttctgg gaaaaagaat caagcggcag gaaaaattat tgttcaacaa
    69601 aatccagcag ttgtagcgga gcaagaaaac tctaaaaaaa gcttagaaga agtatataac
    69661 aaagctaaga aagctatttc tgatgatgag tggttatcgg atgaggaaaa gagagttcag
    69721 actgcagcag ttgaaaaaac ttataatgaa ggtcagactt caattaataa tgcagaaaca
    69781 gtgactgaaa taaaggatgc tttcaaaaaa tattctacgc aaggtaataa aatttctata
    69841 cctgatcaat ataaaaaagg cgaaaaagct ataaaacaag aacaagctcg aaaaagtttg
    69901 aagcaggtac acgaagatac attaggaaaa attagatcag atgattggtt gactgaagaa
    69961 gaaaaagcaa ctcaaacaga gaatgcaaaa cagagttata atcaaggaga gaacaagatt
    70021 aatcaatcag at
//
LOCUS       DAUCWO010000003        64375 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUCWO010000003 DAUCWO010000000
VERSION     DAUCWO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64375
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.003"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            143..3217
                     /locus_tag="VGY20_000160"
     CDS             143..3217
                     /locus_tag="VGY20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4273049.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPAATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYISSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYYRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVTY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDEGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(3248..4234)
                     /locus_tag="VGY20_000161"
     CDS             complement(3248..4234)
                     /locus_tag="VGY20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEQ4273050.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGIDPN
                     LAVLVLQHIAASRKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(4421..5962)
                     /gene="hutH"
                     /locus_tag="VGY20_000162"
     CDS             complement(4421..5962)
                     /gene="hutH"
                     /locus_tag="VGY20_000162"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ4273051.1"
                     /translation="MTRVINLDGESLTLEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASTVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(5974..7317)
                     /locus_tag="VGY20_000163"
     CDS             complement(5974..7317)
                     /locus_tag="VGY20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ4273052.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPATIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDAPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPEEQI"
     gene            complement(7537..8133)
                     /locus_tag="VGY20_000164"
     CDS             complement(7537..8133)
                     /locus_tag="VGY20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ4273053.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDMNLMYDDQYQGQLLPVWSDTLFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCLNQTSPAQTPVAVWAGLWYKT"
     gene            complement(8155..9828)
                     /locus_tag="VGY20_000165"
     CDS             complement(8155..9828)
                     /locus_tag="VGY20_000165"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ4273054.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKGKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGEKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLT
                     KIVTKVYGGKGITLSPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGTIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(9846..10472)
                     /locus_tag="VGY20_000166"
     CDS             complement(9846..10472)
                     /locus_tag="VGY20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ4273055.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQVIIEDLHTKSSRLQQDLLDAIDKDTEAFNLVSAVFDMPKETDADKA
                     ARRTGMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(10483..11382)
                     /gene="ftcD"
                     /locus_tag="VGY20_000167"
     CDS             complement(10483..11382)
                     /gene="ftcD"
                     /locus_tag="VGY20_000167"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ4273056.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQVLENHLLG"
     gene            complement(11470..13500)
                     /locus_tag="VGY20_000168"
     CDS             complement(11470..13500)
                     /locus_tag="VGY20_000168"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002914331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ4273057.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRFRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVSQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVTTDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVNQLFQ"
     gene            13790..15055
                     /locus_tag="VGY20_000169"
     CDS             13790..15055
                     /locus_tag="VGY20_000169"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ4273058.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGDPKAYLDVIIKDMLPVVKEKNLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITNDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYLFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(15113..16645)
                     /gene="ahpF"
                     /locus_tag="VGY20_000170"
     CDS             complement(15113..16645)
                     /gene="ahpF"
                     /locus_tag="VGY20_000170"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ4273059.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHDSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(16666..17226)
                     /gene="ahpC"
                     /locus_tag="VGY20_000171"
     CDS             complement(16666..17226)
                     /gene="ahpC"
                     /locus_tag="VGY20_000171"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ4273060.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYKTLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            17632..17702
                     /locus_tag="VGY20_000172"
     tRNA            17632..17702
                     /locus_tag="VGY20_000172"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17664..17666,aa:Cys,seq:gca)
     gene            18211..18414
                     /locus_tag="VGY20_000173"
     CDS             18211..18414
                     /locus_tag="VGY20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ4273061.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            18610..19071
                     /locus_tag="VGY20_000174"
     CDS             18610..19071
                     /locus_tag="VGY20_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ4273062.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            19071..21515
                     /locus_tag="VGY20_000175"
     CDS             19071..21515
                     /locus_tag="VGY20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4273063.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKPPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            21693..21983
                     /gene="groES"
                     /locus_tag="VGY20_000176"
     CDS             21693..21983
                     /gene="groES"
                     /locus_tag="VGY20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ4273064.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            22019..23650
                     /gene="groL"
                     /locus_tag="VGY20_000177"
     CDS             22019..23650
                     /gene="groL"
                     /locus_tag="VGY20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ4273065.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            23902..24147
                     /locus_tag="VGY20_000178"
     CDS             23902..24147
                     /locus_tag="VGY20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273066.1"
                     /translation="MNHFGEIFKTFRESRGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDTTQDSDGLK"
     gene            complement(24852..26351)
                     /locus_tag="VGY20_000179"
     CDS             complement(24852..26351)
                     /locus_tag="VGY20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ4273067.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGISMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPKNKKGLNRS"
     gene            26598..28238
                     /locus_tag="VGY20_000180"
     CDS             26598..28238
                     /locus_tag="VGY20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273068.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNHKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLIGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYMPDPGSDISALVPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(28327..29328)
                     /locus_tag="VGY20_000181"
     CDS             complement(28327..29328)
                     /locus_tag="VGY20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4273069.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKNPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            29904..30284
                     /locus_tag="VGY20_000182"
     CDS             29904..30284
                     /locus_tag="VGY20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ4273070.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            30323..32659
                     /locus_tag="VGY20_000183"
     CDS             30323..32659
                     /locus_tag="VGY20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4273071.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            32708..32860
                     /gene="rpmG"
                     /locus_tag="VGY20_000184"
     CDS             32708..32860
                     /gene="rpmG"
                     /locus_tag="VGY20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4273072.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            32874..33050
                     /gene="secE"
                     /locus_tag="VGY20_000185"
     CDS             32874..33050
                     /gene="secE"
                     /locus_tag="VGY20_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ4273073.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(33287..34060)
                     /locus_tag="VGY20_000186"
     CDS             complement(33287..34060)
                     /locus_tag="VGY20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ4273074.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            34186..34932
                     /locus_tag="VGY20_000187"
     CDS             34186..34932
                     /locus_tag="VGY20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4273075.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            34948..35928
                     /locus_tag="VGY20_000188"
     CDS             34948..35928
                     /locus_tag="VGY20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ4273076.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRQQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            36216..36536
                     /locus_tag="VGY20_000189"
     CDS             36216..36536
                     /locus_tag="VGY20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4273077.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            36564..36872
                     /locus_tag="VGY20_000190"
     CDS             36564..36872
                     /locus_tag="VGY20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4273078.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            36882..38186
                     /locus_tag="VGY20_000191"
     CDS             36882..38186
                     /locus_tag="VGY20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4273079.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIVSGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            38397..40814
                     /locus_tag="VGY20_000192"
     CDS             38397..40814
                     /locus_tag="VGY20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ4273080.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFALNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGYGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            40827..41495
                     /locus_tag="VGY20_000193"
     CDS             40827..41495
                     /locus_tag="VGY20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ4273081.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     NSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            41552..42640
                     /locus_tag="VGY20_000194"
     CDS             41552..42640
                     /locus_tag="VGY20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ4273082.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMTGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(42722..43231)
                     /locus_tag="VGY20_000195"
     CDS             complement(42722..43231)
                     /locus_tag="VGY20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ4273083.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            43595..44410
                     /gene="sdaB"
                     /locus_tag="VGY20_000196"
     CDS             43595..44410
                     /gene="sdaB"
                     /locus_tag="VGY20_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ4273084.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(44651..45493)
                     /locus_tag="VGY20_000197"
     CDS             complement(44651..45493)
                     /locus_tag="VGY20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4273085.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            45992..46162
                     /locus_tag="VGY20_000198"
     CDS             45992..46162
                     /locus_tag="VGY20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273086.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            46434..47630
                     /gene="speB"
                     /locus_tag="VGY20_000199"
     CDS             46434..47630
                     /gene="speB"
                     /locus_tag="VGY20_000199"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ4273087.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            47632..47937
                     /locus_tag="VGY20_000200"
     CDS             47632..47937
                     /locus_tag="VGY20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ4273088.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQISSLD"
     gene            48422..49351
                     /gene="prsA"
                     /locus_tag="VGY20_000201"
     CDS             48422..49351
                     /gene="prsA"
                     /locus_tag="VGY20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ4273089.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTTAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAPK"
     gene            complement(49628..49864)
                     /locus_tag="VGY20_000202"
     CDS             complement(49628..49864)
                     /locus_tag="VGY20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273090.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPTAHGPCD
                     DTHQLLEDKSLSDDYLISTSAMRHLWYWGYSQIG"
     gene            50288..53338
                     /locus_tag="VGY20_000203"
     CDS             50288..53338
                     /locus_tag="VGY20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4273091.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSVSTETSASTST
                     STASAGTSTSETAASGTASGTNGASSVTSSEGSQSSESAPASPQPQAQTAVASSSSSS
                     NANASSSSDGKAPQAASTTSSSTPAAASSNGISQGTSAEAETPMMEVEQYTVGNKTTE
                     LNIKDGAQLKNGSKVDKDTKLIRNRDGEQRDIVDIKREVKTNADGTVDVTVTVTPKEI
                     DKGADVMALLDVSKKMTEDDFNNAKDKIKKLVTTLTSKSPDSQPNHNARNSVRLMTFY
                     RKVNDPIELNADNVDAKLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKGKRKKSGKRQ
                     HIVLFSQGESTFSYDINDKDENDTVQKNRIAGKVTTSNPLFPWLPIFNHTNQKAEVID
                     DVDKLLGLAEKMGISLPDGLRAGVKAIGAGSSFLSTFTGSGLTEYLTLNEYRSDILKE
                     KQFDYTKRVGEGYYYHSYSKRTHGDKMPFEKQIREVLERFLPKIEDKEWPKKFIEIFG
                     LQEKEVNQIGVDVIMKVINSIFYKREYHYYNHNLSAIAEAKMAQKEGITFYSVDVTDS
                     DKASKRVKRQVGNEQSKKKKEDAEKDRNNKFDDYLKKMSEGGNFLSKVEERGKFKDTL
                     TELTIKDEFTNKVKVQEDSEGKKYKTGLISGTSNVTHTPAKTGFFSSNESLTWTISKE
                     QLKKAFEDGTSLTLTYKLKVENDKFKKSLEEDNKKKRAKRSAPTENENSVTEKIISNT
                     TTYKINEQKVEGNKFDDVNLTYSKFKVPVPQIDGQVIEPQAPKLPDLPPVIEHGPVLD
                     YTEESIYRLPLEHGSNAPDTQVTIEEDTVPQRPDILVGGQSGPVDITEDTQPGMSGSN
                     DATVVEEDTAPQRPDILVGGQSDPIDITEDTQPGMSGSNDATVVEEDTAPQRPDILVG
                     GQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPKEQPSLSLPQA
                     PVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            53518..55449
                     /locus_tag="VGY20_000204"
     CDS             53518..55449
                     /locus_tag="VGY20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4273092.1"
                     /translation="MTKKFIKSAMILGLASITISMTQPVEAIANRLEYQAQYERVKEE
                     LDALKATSPKLGERSLYFRLVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYKHAKNKLDLAVAGYELYLAREKERGDTPDYQVTQLTFKQLETIKTKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKTSAIDGKAQPFLVNGYYDVFYKQFLFTNKNDG
                     YNYNKEADNLTLYQDLIKELLGLKAEIDRLTKDKTDDSRLETLMWESKFTLSDFRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVDIKEPEKAKQYHVED
                     ETSPSQPEAPAPGQKPAEDEGSSQATEPANPSKEDSSTDASQGSHDSENPATDNPSQP
                     QVPDQGNHQSQVPNDKPQTDKTDTPNVPASPQDTPNAPAPPQDTPKVLEAGGQSGPAG
                     NAEEKAPDSGPKESGQSSSKESPSVGEDTTPNQPDVLVGGQSEPNDITEDTITDAPPT
                     VSGHNASTQPQSVVEDTAPQRPDVLIGGQSEPIDITQDTQPGMSGSNDATVINEDTKP
                     KRVFHFGNKEPQASEKATEQPAVPHDSHTTPQASDDTAAPHLPASGDKPEKVFALAAL
                     TMVAAAGLLSKKRRNTDKN"
     gene            55582..55878
                     /locus_tag="VGY20_000205"
     CDS             55582..55878
                     /locus_tag="VGY20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ4273093.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            56025..56309
                     /locus_tag="VGY20_000206"
     CDS             56025..56309
                     /locus_tag="VGY20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ4273094.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            56733..58001
                     /locus_tag="VGY20_000207"
     CDS             56733..58001
                     /locus_tag="VGY20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ4273095.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            57988..58662
                     /locus_tag="VGY20_000208"
     CDS             57988..58662
                     /locus_tag="VGY20_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273096.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            58675..59892
                     /locus_tag="VGY20_000209"
     CDS             58675..59892
                     /locus_tag="VGY20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273097.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            59986..60639
                     /locus_tag="VGY20_000210"
     CDS             59986..60639
                     /locus_tag="VGY20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4273098.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            60636..62024
                     /locus_tag="VGY20_000211"
     CDS             60636..62024
                     /locus_tag="VGY20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4273099.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            62126..63754
                     /locus_tag="VGY20_000212"
     CDS             62126..63754
                     /locus_tag="VGY20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4273100.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNPSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKPSHNHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFGLWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGALDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            63832..64083
                     /locus_tag="VGY20_000213"
     CDS             63832..64083
                     /locus_tag="VGY20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273101.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKVGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
BASE COUNT        20352 a        12877 c        13461 g        17685 t
ORIGIN      
        1 atgaattccc ttattttcga gatgactagc ttctttgttt attggtaagt gttaagacaa
       61 gcttttgctc attttgggtg acacttgcca aatcgtttta tttaagatat agtgaacttg
      121 actaattttt ggaggcgttt tcatgacgaa gggtattcga tttcaattgc ttggcagtcc
      181 ccacattttt cttgatgaca aagaacaatt ttttgccttt gccaaagcaa atgctctcct
      241 ttattactta gtggttaatg gatctgttag ccgcgaggtg gcggcgagtc tactttggga
      301 aaataagaac acgcaaacag cgaaaaagaa tttacgaaac gctatctacc aggtcaataa
      361 agtcttgcaa gctgatgtga ttatttgtcc caatcgcaac ctgctggttt tgaataaaac
      421 acttgatatc aaaacagata ttaacctttt tctggctgat ccgctagctc acttagaact
      481 ctaccaaggg gagtttttgc agggctttta ccttaaaagt ggtgaggaat ttgatctttg
      541 ggtttcaaaa atgcgtatgc agtatgaaca ggtataccta aaagcgtgtt accaaaaaat
      601 tgaagaaaag ctctctttag atgccattga agatgtcgag gaacatttaa aacagcttat
      661 tgaacgtgat gaatttgaag agaaaaatta tcagctcttg atgaggcttt atcagcaggg
      721 gaattgtcct ggtaaggtca tcgaaactta ttatcagttg gctaatgtgt tggataagga
      781 gctagggatt cagccaagtt tgcaaagcca acagatttat caggaagtgg tcgctaaaga
      841 tcgtaatgaa cgcaaaatca agcatttttt gcgcaacagt aatcattttt tggggcggat
      901 tgatgagatt aagcagctgg aaaactattt tgccaactgt ttggcctgcc aagaggtagg
      961 agctctgctc ttaattggtg atacagggat tgggaaacga accctagcca gacaggtctt
     1021 agccaatcaa acccaaacgt ttcaaattgt cacagctaaa tgttttcgtg aagaggctat
     1081 ggattcctta ttgccttggc gtaatatctt agatggcctg ggagatttgg tgattcaaaa
     1141 ccgcttattg accaccaaag cttggaaggc tgctcttaaa cgctgttttc ctgcggcaac
     1201 catttttcaa gaagataaca accaaccctt tatcaaggac cacaccagct tgttggtttc
     1261 ctttattgtt gatattttac aacatttggc agaaatcaag gcgctggtaa tccttattga
     1321 ggattgtcat tggatggatg aggacagttt gaccttgttg caacgggtca tgaatcaatt
     1381 ggtccactat ccgattgctt ttgtcttgac caagcatcta ggaacgactc ctgagctcgg
     1441 tctttgtttg aatgctttga tgagtcaggg gcgtttagaa agcatttgtt tggagccttt
     1501 taatcggcaa gaaagtttgg cttatattag cagtcaattg ggtagtcagc cagtgacgga
     1561 agaagaaatg gagcacctct atcaagccag ccaaggcaac cctttctttt tgtcagaata
     1621 cactcaggcc ctactgcgtc acgaaaaatt tgtgccttta acgcctgcca ttaaggctaa
     1681 gttgggtctt aaactagcta atctaagtag tcgtgatgac gcattgttaa actatttgtc
     1741 ttgttgtcgg aggcctatcc ctctaaatac cctcgctcaa ttgatgttac tgcctttaga
     1801 agaagtgatc gagatggtgg ataatctggg gcactactat attttggtag aagaaagcgt
     1861 aggagaggag gtgttgattt catttcgtca gcgaattatt caactctata gttattaccg
     1921 tttatctttg tcaaaaaggc gtttattgca cggacaaatt gccaaacggt tagaagattt
     1981 actgcctatt ttgacaccaa gccctcactt attagatgac attgcttacc attatcagga
     2041 atcacggcag gtgattaagg ccttggaata caaccttaac tatctagatg ctaccttgcc
     2101 atttcagcat gagctgtttc ccatttattc aaagagtatt ggctccttgg aaaagtcaga
     2161 tcgtgaccat caacgcttaa tggaagagca gtttgataag attcggcaaa gcattgcgga
     2221 tttggaactg acctatgata ataaccgtga tttccagcag ttgcttattc gtttttctta
     2281 ccttgaaggc cgttatgata ttcgaacagg gaggtatcaa gaagggatta aacatattca
     2341 aaaggtcatc gctttggcaa ctgagctaaa acagccctcc ttcttgctag aaggctatcg
     2401 ccagttaatt cattactgta tccaagtgga aaacaagcca gaaatgcgtt attacactag
     2461 tctttcattg gaggcagcgg ttgctgctaa tcattttgag gccattgcga ttagtttgcg
     2521 gttaaacggt ctttatcatt tgattattgg ggagttaaat gaggcagagc gtctgcttca
     2581 acagtcgatt gacttcttta aggtgacgcc aggcttgcaa gctaactatg cgattcagat
     2641 tgctgctgcc ttagattatt tgggggagat tgcccagatt cgctatcagt ttgaaaaggc
     2701 cgttacttac caaaaacaag ccattgcctt gaccgaaaac aagccagctg agttatcggt
     2761 gagtattttt tatattggat tagggatttc ctacttttat ttagctgatt ttgaacaagc
     2821 tgagcaaatc ttgagtttgg ctaaggaagc tttggtcaat catagttatc cttggaaaga
     2881 aacgcaactt gaaatttacc tggccatgat tcaatggaaa aagggtaatt atcagccagc
     2941 cttgacccta cttgattaca gagaaacctt gatgtctagg tatcgcaatc cgcgtgatga
     3001 gggcttggtt ttctatttga tggctgtggt caagtaccaa ttgatatttc aaggagctac
     3061 cctaagtcag caggaaaagg aaatggcaga ccacctgttg tcagaatcct ttgagtatta
     3121 ttatgaaatt gctagtacta acctcaaccc ttaccgagat tgtcatttgg tgagtgagtt
     3181 gaacgatttg cgtcaacaat tatctgctaa aagctaagga aaaccctctg aagtgaatca
     3241 gagggtttta gctatgttgt gccataatct gtacgaggta aaagataaag gtggctgcta
     3301 aattggcggt gtggttatca atatcgtgtg gtggtgaaac ctcaaccaca tcaaagccaa
     3361 ctaattttct actagctgca atgtgttgca aaactaagac ggcaagattt gggtcaatac
     3421 cgagggactg gatagcacta accccaggtg cagcaccaac tgagaaacaa tccatgtcga
     3481 tagtcaggta gaccctttct tgcccttcta agaaacggtc aatggctcgg cagacctttt
     3541 gatgacccat ttggtagatg tcttgaccag tcaaaaattg aatgcccttg gattttgcca
     3601 caaagtcaaa taggaaaagg ttgttattat gttcttgaat gcctaaaacg aagtatttaa
     3661 agaggcgttt gtcagcaacc gcatcgtcaa acatttgtcg aaagcctgtc cctgaattag
     3721 ggcctgtttg gtcgtaaggc cttaaatcaa agtgggcgtc catattgatg acagctaggt
     3781 catcacttgg ggataggctt tggcgtaggc ctaagtagtg gccgtaggcg gtttcatggc
     3841 cacctcctaa gacgatgggt ttgagattga ggtcacacat ccgtttgatg gctttgctta
     3901 aactgttttg cagttgttcc aaagagcgat tggggccgtc aatgttaccc acatcgtaaa
     3961 ccataacttg gtttcctaaa tgccaaggga atttggcgag ctgggttcgt atagcagctg
     4021 gactttcgac cgctccgaca cgaccattat tgatataaac ccccttatca cttttaaagc
     4081 caatcaaggc aaaatgtgtc ccttcaaatg gggttagtga gctgtcgtta aggtcaagga
     4141 aagtcatgac catgccccat ttggcagtat ataagtcatc gtcgatccca ccatgataat
     4201 agctagtggt ggatgggtag taatcttcta gcatgcgcgt catctccttt aggattatga
     4261 tagcaaaata aaacttacag tcaactctct ataggctgtc atcactggtt tttaggaata
     4321 gcagagcaat aaaatcgtct gcttttggtc ccagttagtt agtctaaaga ggccatgacc
     4381 tctgccacag cccctctcag ttaaagaagt accttattca ctaaaattgg atactcaact
     4441 caactgcctg ctcaaccgct tcgaggaagc ttgggtcttc aatgactgtt gatgccttgt
     4501 ttagttcatc gtagatttcg atgtgtttat cgtgttcgat aaagttgact tccttgcgga
     4561 agagatcgta ggcaacctta gtccctttac ctagttcatg attttcaggt ttgagatcaa
     4621 gagcttggca ggctgccatg atttcagtag ccacgatgcg gcgagaattt ttaaggattt
     4681 caaaggcctt gcgagcagcg gtggtgccca tgctgacaaa gtcttcttgg ttctcacaag
     4741 atgggataga atccacactg gctggatgtg ccaaaacctt gttttcagaa gcaagagagg
     4801 cacaggcgta ctgggtaatc ataaagcctg aattgagacc tgggtatttg acaaggaagg
     4861 atggtagttt gctgagctgg ctattgacca aacgttccac acggcgttca gaaacatttc
     4921 caatttcaga aatggcaatg cctaagaagt caaatggctg tgccattggt tctccgtgga
     4981 agttacctcc tgaaataacg tggccatctt tacagataat tggattatct gtgacagaat
     5041 tgatttcaat gtcaactttg gatttgacat aggcgatact gtctttgcta gccccatgaa
     5101 tttgaggcat gcagcgcaag gtgtaagggt cttgcacacg agattgagta gctacggttg
     5161 tgttttggct gccttcaagg aggtttcgga tattgcgggc tgttgccaac tggccgcttt
     5221 gtgggcgaat ggtgtgaagg ttttcttcaa atggactagt aatcccgtta tgcacttcaa
     5281 gggatagggc accagctagg tctgacagtt tcaataattg aatggcgtca taggttgcaa
     5341 gggcaccgat tgcagtgaga acagttgttc cgttgataag ggcgagtcct tctttagcgg
     5401 ctaaagaaat cttgtcaata ccagctttat caagagcttc ttgaccagaa agaagctctc
     5461 ctttgtagta agctttccct agaccaagca taggaagcac catgtgagca agcggcgcca
     5521 aatctcctga agcccctaga gacccttttt caggaatgta aggatgaacc cctttattga
     5581 gcagttccaa cagcttttca atggtggata gacgaatacc agaataccct ttgacgagag
     5641 agttgatacg gatcagcatg atagctctca cggcatcttc tggcaatggg tcaccaaatc
     5701 cactagcatg ggtacgaatc aaattttctt ggagttggac tgtatcttct ggtgagatgc
     5761 tcacgttaca aagggatcca aaaccggtgg tcaccccgta aaccacccgt ttttcactga
     5821 caatgtcatc aacaatttta cgagaggcat tcacagcttc aatagcacta tcatcaatgc
     5881 gacaggctac gccttggcga gcaatcgcaa tcacgtcttc gagggtaagg ctttcgccat
     5941 ctaaattaat cactcttgtc atagtttttt ctcctaaatt tgttcttcag ggggcatttg
     6001 gtccatttgg tggttacgtc cgtgcataag gtagtacaca ctacttgcaa tgatgacacc
     6061 aactgcacca tagacaaagt tcattgggtt gtcaccccaa atcataacta aactaacgat
     6121 aactgcaaga atcgggataa tagggccaaa tggtactcgg aagatcacat tagcatcagg
     6181 agcatctttc cgtaatttca taacggctag tgctgttgga atgtattgga agaagcggaa
     6241 aacaacactg agttttgcta agttttcaaa tgagccagtg agtaggagta caatagcaat
     6301 tgctcctgaa accaagatag caacgagtgg cgcgccattt tgattttgct tggcaatagt
     6361 agctggtaag agcccttcat cagcaatggc agcaccgtaa cgtggcacca tgatggattc
     6421 tcccatgtta agacctgtaa tggaaattaa agcaccgatt gagaccatcc aagcgccagc
     6481 agggcctatc attttaacaa aggcatcttg aacaggtgca ttggtcatca tgatttgaga
     6541 gccaagcatg gcaatggttc caccgataat cagcatatat aagacagaga cgatactgat
     6601 tgatcctaaa agagcacgcg gtacattttt ttcagggtca cgcatttccc cagcgacaat
     6661 agagagggtt tcaaaaccga taaagccata aaagatataa acagcagtat tggagatagc
     6721 ccctaagagg ttagtccctg gttccaactg aacaaatggg gtaaagttag gaagcccgtt
     6781 tttgatgaag aaaagggtac aggcacaaaa ggcaacgatt ggaattaact tagcaatggt
     6841 ggctgtgatg gtgacaattt tagaggtttt caaaccagcg atattcatca aagatagtaa
     6901 gatgattaac ccaatgctaa gtggaatgtg ccaaccttca aaggcaggga aagtaatgat
     6961 aaacattctg gcaaacccag ctgccatggc tgcccaagcg aagatggtaa cggtccaacc
     7021 aaggaagcca acgttaaagc caataaaatc accaaaagct cgtttggaat attggaaggc
     7081 tccgccgttt ttcccaaaat aaccagagac ttctgcaaaa caaactgcca acatgatggt
     7141 caaaatagct gttccaaaca taacagcgat ggatgcaggg ccaaggccct tgtaaatggc
     7201 cctaggtaag agaaaaatac cagagccgat aacggcattg atcccgtaaa gagtggcacc
     7261 gctcaagcta aactttgcct gttctctttc ttgttcgttc atgtgatttg acgccataat
     7321 aatgctctcc tttttgtttg caaattgcgt tcaaatataa tggataaggt cgctaggaaa
     7381 tgaggagata gtcggtttag gagggtcctt cttgcaccta acgatagtga tgagctattg
     7441 gttagcgatg agaaataggt agccttttgt gatgatgtgt agtgatttca ttggagaaga
     7501 ataggcatga gtagtgtgat gcctatcttt atcccactaa gtcttatacc ataagcctgc
     7561 ccaaacagct actggagttt gggcggggct tgtttgattg aggcaaactt ttagatctga
     7621 aaggctggcc tcctgctcga ttaccaacaa gtgattggct tcaagctctt gattcgcctg
     7681 attggtcaca gaataagtca gtttgcttag ggcataaagc agttgacagt ccgctttagg
     7741 tgcctcaaat agtgtgtcag accagacggg taataattgc ccttggtatt ggtcatcata
     7801 cattaaattc atgtcttggc agattccacg actgacgatt ctggtatcgc cttcaaacgc
     7861 ataaggttca aaaggagtta gggaaatctc tttagcatga ctcaaatcac tcaatgtgat
     7921 gggtttgtct agggtcatta gaatcctgtg atagccatca agtaaggtaa aggttgtctg
     7981 tcgtagggcc acagttgact ttgagaggcg ataagagaat tctctctttt tgtaatctcc
     8041 atcttcggga taaaggaaca attggttggt ttcaccgcca gaccagtcag tttggaggta
     8101 atcagagggc gctttgatag ttacctttgt catttgttct ctccttgttt ccaattagaa
     8161 tagtcctgtg atgttgccct cctcgtcaat atcaatggtt tcgctggcag gtacttttgg
     8221 aaggccaggc atggtcatga tagtacccgt cagagcaacg ataaatcctg ctcctgcaga
     8281 cactttgagg ttgctaatag tcactgtaaa gtcagttggt gcgccaagtt ttttggcatc
     8341 gtctgagaag gaatattggg ttttggccat gcaaattggg tagttgccaa agccgaggcg
     8401 ttctaagtca gccagttcac gtttggctgc tgggctcaag gtgatgcctt taccaccgta
     8461 aactttggta acgattttag tcagtttggt ttcaatgcta tcgtcttcct cataaacaaa
     8521 gcggaattga ttgtcttgct cggctagtgt cacgactttt tcagctaatt cacggccacc
     8581 agctccgcca tttgcccaaa catcagaaat cacaacgtca acgccacgtt tgtcgcaggc
     8641 atcatagaca gcttgtaatt cagcatcggt atcaagcggg aatttattga tcgccacgac
     8701 aactggaagg ccatagacgt cttggatatt agccaaatgt ttgtcaaggt taggtaaacc
     8761 atccacaacg gcctgaacat tttcagtagc caggtctgct tttggcacac ccccatgcat
     8821 tttaagggca cgaatagtgg ctactaggac aacggctgct ggacgaaggc cggacatgcg
     8881 gcatttgatg tcaatgaatt tttcagctcc aaggtcagca ccaaatccag cttcggtaac
     8941 ggcataatca ccatatttca aggctagttt ggtggctagg acactattac agccatgggc
     9001 gatattagca aatgggccac cgtgaatgag ggctggtgtg tgttccaggg tttgcacgag
     9061 attaggatga atcgcatcct tgagcagtgc ggctagggca ccaccagctt tcaagtcttt
     9121 agctgttaca ggttcgcctt gatagttgta accgatgatg attttttcaa ggcgggcttt
     9181 gaggtcagaa atattttcgg ataggcacag gattgccatg atttcagagg cgacggtaat
     9241 gtcgtaacca tcctcacgag ggaccccgtt gaccttgcct tgcaagccgt caacaatatg
     9301 acgaagctgg cggtcattca tatccaccac acgtttccaa gtaatacgtc tggaatcaat
     9361 gcctaggcta ttgccatggt gaatgtggtt atcaatcaag gcagctaata agttgttggc
     9421 gacaccgatg gcatgaaagt cccctgtaaa gtgaaggttg atgtcttcca ttgggacaac
     9481 ttgcgcgtgg ccaccgccag cagctcctcc cttaacccca aaaacaggac caagagaagg
     9541 ttcacgaagg gcaataactg ctttttctcc aatggcagaa agagcatcta ccaatccaac
     9601 agaagtggtg gttttgccct caccagctgg tgttggtgag atagcagtga caagaatgag
     9661 tttgccatca ggtttgcctt ttaaggcgac cagttgacgg gcatcaattt ttgccttgta
     9721 cttaccatag agacagagcg cttccttgtc aatccctaat tgatcggcaa ccttactgat
     9781 aggttccata gtgacagagt tggctatttc aatatctgat aaaaccatag ttgctccttt
     9841 acttgttaaa caatatcgag aattttcgtg tagatgtcat ctgccaaatg acagcccttg
     9901 tctaataagg cttgtccttt ttggcgatag tcggtaacaa agtcttcatc tttgatgcca
     9961 gacagattaa tcaaaacatt caaccaagcg ccttgtaaac ctgcttttaa gtttaaagca
    10021 gcaactccta agtcgctagc agcatttgta ttagacttac cgacagctgt tgcagtgatc
    10081 tcaagtgctt cgaccatgag ggtcatcatc tcaaaaggtg attgtgcagc tgttttcaag
    10141 gccttttgca tgccagttcg tctggcagcc ttgtctgcgt cagtctcttt tggcatatca
    10201 aaaacggcag aaaccaggtt gaaggcttcg gtatctttgt caatggcatc taataagtcc
    10261 tgttgcaagc gactactttt ggtatgaagg tcttcaatga taacttgata atcagcatac
    10321 tttttcttac caagagtcaa ttcacagacc atcttggtta aggaaatgcc attagcgcca
    10381 gagagagcag cagcagaacc accacctgga gcaggggcat ctgagccaag gactttagcg
    10441 aaatctgtta agcttaaatc aactaaaccc ataagacacc ccctagccta ataaatgatt
    10501 ttctaacact tgtttatggt agtcaaagtc ctcaacttgc aagtagtatt cagccacatc
    10561 gattaaggct ttggctggcg ctaagccgat gatttcagaa ccaataacat tcacaccgta
    10621 gcgacgcgct tcaaatttga tggtttcaaa agtacggtaa agagaacatt tttcaaagtt
    10681 aaccatgttc atggagactt gggcaatatg acggtcttct aacatgacac cgatggcttt
    10741 acaatatttg tagccaccac ctgaaccgcg gataatttta gcaattttat gagcaatgtc
    10801 gatattgtca gtatccaaat taacattgaa agcaaccaat ggcatgcgag caccaacggc
    10861 agttacccca gcagttgggt gaatcttacg atctccgtaa tcaggtgccc aatcctcttc
    10921 caacagtttt tctggcatgc cttcgaattg tcctttacgg actttggcca ggttttgacg
    10981 ttctggacgt gtggcagaat cttcataaag gaagattggg attccaagtt cacggttaat
    11041 ccgttctgcg acttgtttgg aaatttcgac acattcttgt gtagtgatgt ctttgatcgg
    11101 gacgaacggg cagacatcgg ttgctcccat acgagggtgt tcaccatgat gtttggtcat
    11161 atcaatgttt tcagaagcgt atttcaccag ttggaaagct gcttcttgaa tagactggtc
    11221 atctccaact aaggtaaaca cgctccggtt gtggctcgca tcagaagagt agtcgaggag
    11281 ggtcactcca ggaatacttt tggccgtcgc taccaagccg tcgataacag cctgattttg
    11341 gccttctgag aagttgggaa tacattcaac aattttcgcc atgttaatct ccttttatgt
    11401 cattgagtta ctcattaagg gaatgggtta cctattatag ataacgctta catttttatc
    11461 tagctgcttt tactgaaata attgatttac cgctgaagtc acaaggtcat cgtcagccaa
    11521 atatggaata gtgatgtgat cagtacctgc atggaggcgg ttgtattcaa tagcggtttc
    11581 aatagcgtgc tcattgcgag cccaattgcg acgagcaaca ccacccatgg tgtcccaagc
    11641 aatagcagat ttaataatct catcaatgcg ttcgctacca tctagaacaa gtccaaagcc
    11701 gccgttaatg gctttaccga taccagttcc gccaccgtta tgaagagcca ctaaactcat
    11761 accacgcgca gcgttaccag cgtaacattg aactgccata tcacaggtga cattggaacc
    11821 gtctttaatg tttgaggttt cacgaaatgg tgagtcggta ccagacacgt catggtggtc
    11881 acgaccaatc atgacaggac caatcttgcc tttgcggacc aattcattaa atttaagggc
    11941 gatagtcaca cgaccgatac agtcttggta aaggatccgg gcttgggtac caactacgag
    12001 ttgatttttc tcagcatcac gaatccagtt gtagttatca cggtcttgat agcggcggtc
    12061 aggatcgata gcttccatgg cagctttgtc agtagtcacc aagtcttcgt gtttaccact
    12121 caagcagacc caacggaaag gtccatagcc atagtcaaaa agcataggtc ccatgatatc
    12181 ttccacatag gatggccaga taaagccgtc tttgtcgtta cgaccattct tagaaatttc
    12241 tgtaatacca gagtcgtaaa cagacttcat aaaggcattg ccgtagtcaa agaaataggt
    12301 gccattttca gttaacgtct taatagcttc aaaatgccgt gccaaggtat catcaaccat
    12361 ttggtggaag gtatccttgt cttctgccag gagccgggtt ctttcgtcaa agctaatacc
    12421 tactgggcag tagccaccgt cataaacgtt gtgacaagag gtttggtcag acaaaagatc
    12481 aacatgaatc tgcttgtcat tgacgtattc taaaaggtca acaatattac catggtaagc
    12541 aatcgaagtg gactccttag catctatggc tttttgggct aattgcagag cttcttcagg
    12601 gctctcagca atttggctaa cccagccttg cgaatgacgg gtttcaatcc gagattggtc
    12661 aacttcagca ataatggcaa ctgctttagc aatttcagca gccttacctt gtgccccact
    12721 catgcctcct aaaccagatg agataaagag tttaccggtc aaatcaccat cgtcagccac
    12781 tccaagtttg agacgaccag catttaagag agtattaaat gttccatgaa caatcccttg
    12841 tgggccaata tacatccaac cacctgcggt catttgacca tagttggtga cacccatctc
    12901 ttcagcgatt tcccagtctt tcatgttgtc gtattcacca accaaaaggc cattagtaat
    12961 aatcacacga ggggcttcag gtttggattg gaaaagacca acgggatgac cagattcaac
    13021 caccaaggtt tgttcatcag tcatgacctc taggtatttt ttaatcaaac aatattgcat
    13081 ccagttggca caaacagaac cagtttctcc ataagtgact agttcgtaag ggtacaaggc
    13141 aatctcaaaa ctcaagttat tgtcaatcat gacctgcatg gccttggcag ctgtacaatt
    13201 tcccttgtac tcgtcaatgg gtttgccgta aatgcggtct tttggtctaa agcggtagcc
    13261 ataaatacgg ccacgggttt tgagttcttc caaaaattct ggaatgactt cttcgtggaa
    13321 ctttgtaggt acatagcgta gggcattttt taaggcaatt tcggtctgag cctgggtcaa
    13381 tcgaaaacct ctgtctggtg ctcttcggat accttctttg aacacagtct tttccggtaa
    13441 aacatcgtct aacttaacag tcatagcggc agctatgtca gtttcactgt aaaatgacat
    13501 agaaaactcc tcctcaactc ctttttattg tcgagattag tctaacttag cctagtctat
    13561 aaaaagtcaa ccacgctttg aaatgcgaaa aaagtctaaa aaaaatcaaa acctcatttt
    13621 ttgactaaaa tttgacgctt aatcagtaca cttgaggtat gacagataaa gtcagtcaga
    13681 ggttaattgt tgttaccaaa gttgcaaaga tcgcaactat tgagtgggtg aggggggcta
    13741 gtaatggcta ttaatcagtt gacaaacgat gttaaaaaag gaggttttta tggttgctga
    13801 tgtcttatta acacatttta atcaactatt ttgtctaaat gatccaggtc atcctttaac
    13861 tggtcaagaa atgaaaaaag ccactatcgt tgaagatggc tatatagcca tcaaagacgg
    13921 cctcattgtg gctcttggtt ctggtgaacc agatgctgag cttgtcggtc cacagactat
    13981 tatgcgttct tataaaggta aaattgcaac gcctggtatt attgactgtc atacccattt
    14041 ggtctatggt ggcagtcggg aacatgaatt tgccaagaaa ttagcagggg tgtcttactt
    14101 ggatatttta gctcaaggtg gtggtatttt aagcacggtg agggccactc ggtcagccag
    14161 ctttgacaac ctttatcaaa aatccaagag attgctagac tacatgttgc ttcatggagt
    14221 gactaccgtc gaagcgaaaa gtggctacgg ccttgattgg gaaacagaaa aacgccaact
    14281 ggatgttgtt gctgctttag aaaaagacca tcctattgat ttggtgtcta cgtttatggc
    14341 agcccatgcg attcctgaag aatacaaggg ggatcctaag gcttatctcg atgtgattat
    14401 caaggatatg cttcctgttg ttaaagagaa aaatctggca gaattttgtg atattttctg
    14461 tgaaaaaaat gtttttacag ctgatgagtc ccgctatctg ttaagcaaag ccaaagaaat
    14521 gggcttcaag cttcgtattc acgcagatga aattgcgtct ataggcggag ttgatgtcgc
    14581 agcagagctc agcgcagtta gtgcagagca tttgatgatg ataacaaatg atggtatcgc
    14641 taagttgatt ggtgctggtg ttattggtaa tttgcttcca gccaccacct ttagtttaat
    14701 ggaagacact tatgcccctg ctcgcaaaat gatcgatgcc ggcatggcca tcaccctctc
    14761 aacagatagt aatccaggca gttgcccgac tgctaacatg cagtttgtga tgcaattagg
    14821 ttgttttatg ttacgtttaa caccgattga agtcttaaat gctgttacca ttaatgcggc
    14881 ctactctgtc aatcgtcagg agagagttgg tagtttgaca gttggtaaag aagcagatat
    14941 tgccatcttt gatgccccaa atattgatta cctattttat ttctttgcca ccaacttgat
    15001 tcaccaagtc tataaaaaag gacaactcac agttgaccga ggtcgtattc tttaaaaaat
    15061 ttcaaaaaga agctttgagc gttagctcaa aacttctttt aaatatgctg ttttattgac
    15121 gaatcaagta gtcaaaggca ccgatggcag cagtagcacc agaccccatg gaaataatga
    15181 tttgtttata agctgaatca gtacagtccc cagcagcaaa gataccagga atattggttg
    15241 acccatgttt atccacaatg atttcaccgc ggtcggttag gttgacacca ctatctttga
    15301 gccaagctgt gtttggaaca agaccaattt gaacaaagac cccttcaagg tcaaggtgtt
    15361 tgtcttcacc gctatcacgc tcagtgtagc taagacctgt aacatggtcc tctccaacaa
    15421 tatccttagt agcaacattt ttgatgatgg tcatattgtt agtcttagct gcgcggtctt
    15481 gaaggacttt gtcagcttta agttcaggca agaattctaa aacgtaaacg tgtttagcaa
    15541 gaccagccaa atcaagggcc gcttctaaac cggagttacc accaccgata acagcaacat
    15601 ctttgccttc aaagagagga ccgtcacagt gagggcagta agtcacccct ttattacgga
    15661 attcatcttc accaggaaca ttaatgttac gccatttggc cccaagagct aagatagcag
    15721 ttttggcttg tagaaccgca ccattagcta gagtaacctc gatgttttct tttttctcaa
    15781 tagaagtagc tagttgtgct ttgataatat caacatcata agacttagta tgggcctcca
    15841 cttcggccat taattttggt ccttcagtgt aaagggtacc aatcatgttt tcgatgccga
    15901 cagtttccat gacctgacca ccaaaggttt cagcaagtaa acctgttttt agtcctttac
    15961 gagctgcata gatagcagcg ctattgcccg cagggccacc accaataaca aggacatcat
    16021 agaggccttt atcagcaaag gcttcctcag aaagtgggcc agcgatttgt tctaagagtt
    16081 gctcaatggt agcacgacca gaagtgaatt cttccccgtc taaaaagaca gtagggacag
    16141 acataatgcc tttggctttc acttcatctt ggaacatgcc gccttctacc atagtgtgtg
    16201 aaatcttgtc gttcaagacg gacataatgt ttaaagcttg aacaacatct gggcagttat
    16261 ggcaggttaa gctgacataa gtttcaaaat gcaacggacg gtcaatggcc ttgatgcgat
    16321 caataacatc ttgatccact tttggagcac gtccagatac ttgcaagaga gcaagaataa
    16381 aggacgtcaa ttcgtggcct aaaggaagac cagcaaagac aaccccacta tcgtgacctt
    16441 ttttcgcaac cttaaagctt ggttgtcggt ctaaagtgat gttttcaata gaaatacgct
    16501 cagacatagc agctatttct tccacaaagt ctttgacttt ttgagactgc tcattgtcac
    16561 caagggaaac ttgcaaaaca agatcagctt ctaacagggt gaggtattgg gcaagttgtt
    16621 ctttaatatc aggacttaat gccatagctt tcctcctaaa atttattaaa ttttaccaac
    16681 taaatcaaga cttggtgtca aagtttcagc gccttctttc catttagctg gacaaacttc
    16741 acctggatgt ttacggacgt attgggcagc gtgaatttta tcaatcaagg tgctagcgtc
    16801 acgtccaata ccatcagcat taatttccat catttggata ataccatctg ggtcaacgat
    16861 aaatgttcca cgttgagcaa gtccgtcttc gccaagcact tcaaaggctt gtgaaataag
    16921 gtgtgaaggg tcaccaatca tagggtatgt gatagtgcca accacatctg aatcatcatg
    16981 ccaagcttta tgaacaaaat gagtatcagt agagacagaa taaacttcta caccaagaga
    17041 ttttagtgtt ttgtattgct cttgaaggtc accgagttca gttgggcaaa caaatgaaaa
    17101 gtctgctggg tagaaacaaa aaactgccca ttttccttta acgtcttcat ttgtaacagt
    17161 gatgaatttt ccatcgtgat aagcttgagc tgaaaattca gcaatttctt ttccaattag
    17221 agacatatag ttgtcctcct tttttatagt tttatacttg tattataaaa gaacaacaag
    17281 attatgtcta ctcagaaacc cttgatttta ctgggaaatc agcgattgtt ttctgacaat
    17341 tcagttgtga aataaatcac attaattaaa atgattctaa ataagccgaa gacgccgtta
    17401 aatcaaaggt taatttaatt ataatctaca gaaaatataa ttgttattaa agcgtttaca
    17461 aaaaactttt ttgattccgt taaggcaact taatattatg gttacctaat cactaatatt
    17521 gtaacctatt ttggcatgag atcagggaat ggctgttata ttaattggat atgtcttgtg
    17581 caaatagtgc ttgacggatt tgtggaatta gggtaaaata atctttgttg tggcggtata
    17641 gccaagtggt aaggcacggc tctgcaaaag cttgatcgtc ggttcaaatc cgtctaccgc
    17701 cttttatata caaaacaagc cttgattaac ttcgtgaaaa cgttgttaaa tcaaggtttt
    17761 tattttttgc ggcaggataa aaaaagagaa aaaaaaaaat aaagtcggtc aaatttcccg
    17821 aagatgtctt ttagagcctg attattaggt atgaaaaata ctatggccaa aattggtaaa
    17881 cgattgcaaa taaaggtcag agttcttacc cccttaaacg atatgcgtta aaagttagtt
    17941 gaactgccgt gcaccttaac gatagacacg gtgatgtgag agcgagtaga gttgaatctc
    18001 tacttgttta ccttaccata gtcagcttta taaagtaacc acccattaca atgcaactat
    18061 attcttaatg tttttaagca tgttattgac aagtgttata aaatgtgtta ctcttagtga
    18121 gtaaattagt ttttgagtta gtatttgtgt ttcaacatct ttatttctct ctatttttac
    18181 aaatgatttt tagaaaggaa gtggatgtac atggcacaag gtacagtaaa atggtttaac
    18241 gctgaaaaag gttttggttt tatttcaact gaaaacggtc aagatgtctt tgcacatttt
    18301 tcagcaatcc aaactaatgg ttttaaaacg ttagaagaag gacaaaaagt ggcatttgac
    18361 gtcgaagaag gtcaacgtgg acctcaagca gtcaatatca ctaaattggc ctaatctggt
    18421 taaagtaact aaagagaagc agatgttgga aacaacaact gcttttttga tttctaccag
    18481 ttacctttaa tgattaagag ttgcgtttaa ggtcttgtat ttgttataat aatagtcagt
    18541 tttagtcaaa atgtattgac gtggtgctac taagttttta gagtagcccg caagcaaagg
    18601 agtgacagca tgccaaccaa aaatacttca gacagcattg aagagtatat taaagaatta
    18661 ttagctaagt caggaatagc agaaattaag cggtccatgc tagcagattc gtttcaagtt
    18721 gtcccaagtc agatcaacta tgtcattaaa acccgtttta cggagagtcg aggctatgaa
    18781 gtagagagta aacgaggtgg tggaggctat attcgtattg ccaaggttca tttttcagac
    18841 aaacaccatc tgattggcaa tttaatggca actattgaag actgtatcag tgagcaagtt
    18901 ttcacggact cgatacaatt gctttttgac gaacacttat tgacagagcg tgagggaaat
    18961 atcattttgg ctgtggcgtc tgatgatgtt ttaggaactg atggttcaac tattcgtgca
    19021 cgaatgctct atcgcttatt gcaacgaatt gacagaaagg gaagcaatta atgattatgt
    19081 attcaacgaa gatgcaagac atttttagac aggcgcagtt ccaagctgct cgctttgata
    19141 gccattgcct ggaaacttgg catgttttgt tagctatggt agctgtagat aattctttag
    19201 caaatatgat tttaagtgaa tatgatgccc aagtcgccat agaagaatat gaagctgcag
    19261 ctattttagc catgggcaaa ccccctaagg aacagttgtc tcgtgtagac ttcagacctc
    19321 aatctaaaac cttgactaac ttgttagctt ttgcgcaggc tattagccaa atcactaggg
    19381 atcaagaagt cggctctgag catgtcttat ttgctatttt attgaatcca gatatcatgg
    19441 cgagtcgttt gttagaaata gcaggctatc agataaaaga taacggcaat gggcagccgc
    19501 gattagctga cttgcgaaaa gcaatagaac gtcatgcagg ttacagtaag gaaatgatta
    19561 aggctattca cgaactacgt aagcctaaaa aaacgaaaac acaagggacc ttttcagata
    19621 tgatgaagcc accaagtaca gctggtgagt tgagtgattt cacaagagat ttgactgaaa
    19681 tggcaagaca aggtttgtta gaatcggtga ttggacgtga ccaagaagta tctcgtatga
    19741 ttcaggtact aagtcgtaaa acgaaaaaca atcctgtctt ggtaggtgat gcaggtgttg
    19801 gtaaaactgc gcttgcttat ggccttgctc aacggattgc aaatggcgct attccttatg
    19861 aacttaagga gatgcgtgtc ctagaattag acatgatgag tgtggtagca ggaacccgtt
    19921 ttcgtgggga ttttgaagag cgcatgaatc aaatcattga tgatattgaa gctgatggtc
    19981 agattattct ttttgttgat gaactacata ctattatggg ttctggcagt ggtattgaca
    20041 gtacacttga tgcggctaac attttaaaac cagcattatc gcgcggtact cttcatatgg
    20101 ttggagcaac aactcaagaa gaatatcaaa aacatattga aaaagatgca gctctttcgc
    20161 gtcgttttgc taaaatatta attgaagaac ctaatacaga agatgcttat cagattttga
    20221 tgggcctaaa attatcttat gagacctacc ataatgtctc gatatcaaat gaagcagtta
    20281 aaacagctgt aaaaatggca caccgttatt taaccagtaa aaatctccct gattcagcta
    20341 tcgatttatt agatgaagct agtgctgctg tgcaaaacat ggtgaaaaaa tcagcacctg
    20401 agactttaac accaatagac caagctctta tcaatggtga tatgaaaaaa gtatctcgcc
    20461 tcttagctaa agaagcaaaa ggtcagatga gaaaaccaac accagtcaca gaagatgata
    20521 ttttggcaac cttgagtaag ttatcgggaa ttccacttga aaaactgacg caagctgata
    20581 gcaaaaaata cctcaattta gaaaaagaac tgcataagcg tgtgattggt caggatgctg
    20641 ctgttacggc tatttcaaga gccattcgtc gcaatcagtc aggtattcga acaggaaaac
    20701 gtcctattgg atcatttatg tttcttggcc caacaggagt aggtaagaca gaactagcaa
    20761 aggcccttgc agaagttctc tttgatgatg aagcagcgct tattcgtttt gatatgtctg
    20821 agtacatgga aaaattcgca gcgtctaggc ttaatggagc acctcctggt tatgtcggct
    20881 atgatgaagg aggtgaactg acacagaaag ttagaaataa accttattca gtcttgcttt
    20941 ttgatgaagt ggaaaaagca catcctgata tttttaacgt tctccttcaa gtattagatg
    21001 atggtatatt gactgatagt cgtgggcgta aggtcgattt ttcaaatact attattatca
    21061 tgaccagcaa tcttggcgca acagccctgc gtgatgataa aacggtcggt tttggggtca
    21121 aaggcattca ccaagaccat caagctatgg agaaacgtat cttagaagaa ttaagaaaaa
    21181 cttaccgccc agaatttatc aatcgtattg atgaaaaagt ggtctttcat agtctgaccc
    21241 aagataacat gcgcgatgtg gttaaaatca tggtacagcc cctgattact acattggcag
    21301 aaaaaggtat tacccttaaa attcagcctt tggccttgaa acatttgtcc gaggtcggct
    21361 atgatgagca tatgggggca agaccattac gtcgaacgct gcaaactgag atagaagata
    21421 agctatcaga gcttatcctt tctcgagaat tgacaagtgg gcatacgcta aaaattggat
    21481 tatcacatgg caaattaacg tttcacatag cttaatttcc atataaaagg tggatcatcc
    21541 ttgatgtcac caaaaaaaga ggttattctt acaaaaataa cttctttttt cttgactatt
    21601 tttgaccaag tgataaaata gaatataaag ttagcactcg atgaatatga gtgctaactt
    21661 tactaaagat tcttttattg gaggaaaatt tcatgttaaa accattaggt gaccgtgtgg
    21721 tcgtaagatt tgatgatgaa aaagagcaga cagtcggtgg ttttgtcctt gcaggcactc
    21781 ataaagagtc aacacgaaaa gcgacagtcc tcgctgtcag tgaaacgggt gttcgcacta
    21841 ttacaggcga tagcgtgcta ccttcagttt cagtgggtca agaggtttta gttgaaaatg
    21901 ggcatgattt agaagtaacc gtagatgatg agaaagtttc tatcatccgt gaatctgaca
    21961 tcattgctat tgtcacaaaa taaaacgaca aatctctaaa tagaaagaag gattgaatat
    22021 ggcaaaagat attaaatttt cagcagatgc gcgtgctgcc atggtgcgcg gagttgatat
    22081 gttagcagat accgtcaaag taacgcttgg tcctaaagga cgcaatgttg ttcttgaaaa
    22141 agcttttggt tctcccttaa ttactaatga cggggtaacc attgctaaag agatcgaatt
    22201 agaagatcat tttgaaaaca tgggagcaaa attggtgtct gaagtggctt ctaaaaccaa
    22261 tgatattgct ggtgatggga cgactactgc aacagttttg acacaagcca ttgttcatga
    22321 aggactaaaa aatgtgacag caggtgctaa tccaattggt atccgtcgag gcattgaaac
    22381 agcaacagca acagctgttg aagccttgaa agccattgct caacctgtat ctggcaagga
    22441 agctattgct caggtcgctg cagtatcatc acgctctgaa aaagttggag agtatatctc
    22501 agaagctatg gagcgtgtgg gcaacgatgg tgtgattacc atcgaagaat ctcgaggtat
    22561 ggaaacagaa cttgaagtgg ttgaaggcat gcaatttgac cgtggttacc tgtctcaata
    22621 catggtcaca gacaatgaaa aaatggttgc agaccttgaa aacccattta tcttaatcac
    22681 ggataaaaaa gtgtcaaaca tccaagacat tttgccacta cttgaggaag ttcttaaaac
    22741 caaccgtcca ttactcatta ttgcagatga tgtggatggt gaagcccttc caacccttgt
    22801 cttgaacaag attcgtggta ctttcaatgt ggttgctgtc aaagcgccag gatttggtga
    22861 tcgccgtaaa gctatgcttg aagacattgc tatcttgaca ggtggtacag tgattacaga
    22921 ggatctagga cttgaattaa aagatgctac aatgacagcc cttggacagg ctgctaagat
    22981 tacagttgat aaagatagca cagtaattgt tgaaggttca ggaagttcag aagctattgc
    23041 taaccgtatt gcactgatta aatcgcaatt agaaacaaca acttctgact ttgaccgtga
    23101 aaaactacaa gaacgtttgg cgaaattagc tggtggtgta gctgttatca aagtaggagc
    23161 tccaacagag acagctttaa aagaaatgaa actccgcatt gaggacgctc taaatgctac
    23221 acgtgcagcc gttgaagaag gtatcgttgc tggtggtgga acagcactta ttacggttat
    23281 tgaaaaagta gcagctcttg aacttgaggg cgatgatgct actggacgta acattgtgct
    23341 tcgtgctcta gaagagcctg tacgtcaaat tgctttaaat gctgggtacg aaggctccgt
    23401 agttattgac aagttgaaaa atagccctgc aggaacagga tttaatgctg caacaggtga
    23461 gtgggttgat atgattaaaa caggaatcat tgaccctgtc aaagtaacac gatcagcgct
    23521 tcaaaatgca gcttctgtag ctagtcttat tttgacaaca gaagcagttg tcgctaataa
    23581 acctgaacca gcagcgccag cgccagcaat gccagcaggt atggatccag gaatgatggg
    23641 cggcttctaa gctctctata gcattaagga ttatgaaaaa gaagttgaga ctaaaggtct
    23701 gcttcttact atgaaaaaag aacaaagatt ggatttcgac tcatgatatc ccatctttgt
    23761 tttttttagt tgtgatataa aatgccaatc tttgtggtta cagactttta atgtattgga
    23821 aaatatattg tttttctttg tcatgtgcta tcatggagtc aatgaaaagt tattcccata
    23881 tatgcgtggt ggaggtctgt gatgaatcat tttggagaga tttttaaaac ctttagagaa
    23941 tcaaggggat tacgattaaa agatgtcgca aaggctggta tatcaacctc tcagctgtcg
    24001 cgtttcgaaa aaggggagac ggatttaacc atatcgacat ttatgctgat tttggatgaa
    24061 agtaacatgt ccattgatga gtttatgtat gctgtccatg attttcatcg tgacgacaca
    24121 actcaagata gtgatggctt gaagtagtta cttcatgctc aactagcatc aactgataag
    24181 aaggaaatgt tcacggatat cttttggcat atccagcatg tctaagtctg ctatggcatg
    24241 ccattttggg agtccctcac gcgactctgt cagtagctct ccttcaaagg attcacatat
    24301 aaagtcgtaa tagacatagc actctttttt tgtcaggctt gataaagcta gaaataccct
    24361 ttaattgaag gtttaaagct gtcagaccta tctcttcttt tagttctcat aaagccgctt
    24421 caaagaaaga ttcacagggc tcgactttac cttctggctg gccccatcct gggaagttat
    24481 cgtgttgtct atggagtcat aaaatcatat cgttatgttt gacacacata ttgccccagt
    24541 ctctaaccgt tgctatcacc agattcacca ccttttagta tagtataaga aaaaaggggt
    24601 gaaagaggtt tcttgttgtt tttttggctt aagatttctt taagtcttat ctagtatact
    24661 aatgtcattc cttaatagga taagtatttg gttgtagaag tatcgtctta aactaacgct
    24721 atctcaaccg attcccatct tattcacccc tcctataccg ataaggtctc acaaagtgtg
    24781 agaccttatt ttttgacttc agctctagtt atggagaaac ttgttgatac aagctttttt
    24841 gggttatggt attatgatct attcaagcct ttcttgtttt tgggtttttc ttcaactaac
    24901 ttggcttcaa tagcttttaa gcgtttgaag gttttttcag cgcgatctag tgaaatctta
    24961 tcagcttttt cttgagcagc tttggggtca ctgacgacta agtcactgat ttctttggtg
    25021 cttttgtctt gttcggctat ccaggttttt tccaagcctt tcatctcatc aatcacttta
    25081 gttccaaatg gttttggatg agcagccacg agatcattaa tgtgtgacac cgtccaatac
    25141 catgacttat cgttatattg ggtgcttttt tcttgatagg cttcataggt tctactgata
    25201 ttgccaaggt atggcaagta aggggcatta cgaggactgc caatagataa ccacatgaca
    25261 ccaccaccca attcagcagg gatgtctttt ttcagttgga aaatatgagc ttccatgaca
    25321 ttaggattgg aaataggata cgcatagccc ttaacagctt ttttggattt tggtttaccc
    25381 ttaccgtcta atgccatctg atcaagtggt ttaagatcaa gcccctcaaa acgattacgt
    25441 tgcagtttca tcgcatcttc tagactaaag gtcttatcag tgctttggag caactcataa
    25501 ttgctgtctt tataagttac ttttgaatca gggtcaagtg atttaatccc tgagaaagag
    25561 cgagagcggt tggcatcgtt tagtggcgga ttgtaagatt tggcaatatg aaatttacca
    25621 tcaacttcgg tataagattt ggctttttta gcgacttttt ccacatcttc tgaggcaatc
    25681 gtattttcct tatcgttgaa atcgacatgt ccgagataga aggtatttgg aaaaacagcg
    25741 tatttatcat ctgggaattt gatggcaacg tattggtgac cagagagaat ttccatgtac
    25801 cagatgccgt ctttgtcagc caaggtaaca atatttcctt cagcagctcc tttttcagtg
    25861 actattttag caatcaatgc gacaccttca cgggctgttt tgacacttgg taaaatgacg
    25921 ctggccatag atgattccgc taagccattt ttgacgtaag gatcaatttt ttggatggca
    25981 tcattagctg aagctgatac tgttgcagac atagagatgc caaactcatt aaaaccagct
    26041 tcgtcataaa cgcctttatt aggtgttaca tcaggaatcg cactatagcg ataagaatgc
    26101 tcaggcaatg ggtactcaaa accattagag gggtctttcc atttttcgcc agcaggattg
    26161 tcctttgcta gacgtacaat gaagttttta ttatgatgtg gctcaagatc ttctgttcga
    26221 ccataaagaa gagaaccgtc tttggttaaa tctttgccaa taataaaacc agtacaagca
    26281 taactaacac tttgaagcga aaaggctgtc agaatactta aaactccaag tgatattttt
    26341 ttgtttatca tgcgaatcct cctagtaaac gataacgctt tcacagacat tataaaatgc
    26401 tctttttaaa aattcaagtc ttttatgaag tgttttgaaa ttgttagcta tctaaatatt
    26461 atgagaatta tctcttgaat tttggcttaa cggcccctaa aaacgtatag gcagtgaact
    26521 atctaggttt cttcgaaaat ccattagcca tttatgatat aatagtaaag aaaaacatag
    26581 aaagttgggg tctgttgatg accaaaccat ttcatcataa gaaactaaaa caaataacta
    26641 taatagctgc aactagcctt tttttattcc tgatcggtgg tgctttttac tactctaaaa
    26701 atcattgtat taatgcctat cttaaagctc gatcagccca atcaggacca gtttttgaga
    26761 atatcaaagc gtatctagtc tgggatgata ctaatgagca gatcacaaat gacgaggcga
    26821 tgtatactaa gtttagaagg tatagtcaaa aagaattgag gcaaaaaaag caggatttaa
    26881 aagccgctag tcaagatagt gccgttcaag taaagtctgt tggtcgtcgt ttttggattt
    26941 ttcctgatta tcggattgcc ataaaaccca tggatttaac gataaaaacg aatgtacctc
    27001 aagcagacgt tcttttaaat cataaaaaag ttgctgtttc tgattcagaa cagttctcag
    27061 tcaagcttga tcggctacca acggcagaat ataccgcaag tatcagaggc aaacacaacg
    27121 ggcgaaacat taaagtcaac aaatcatatg atggtgataa tcccgtgcta gatttgagtg
    27181 tgtcttttag aacttttttg gtaacaagca atgctaagca aggagatctt tacttcgatg
    27241 ataaccatat tggcacatta aaagatggtc aattacaagt agaagattac cctgttacag
    27301 aaaacgcaca agcttatatg aaaacaacat tcccagatgg tgagttaaga tcacaaaaat
    27361 atgctctagc tgatgttgaa gaaggagcaa ccctggagat tttagttaca gatcttttag
    27421 aagaggacaa ggcaggggag ctattagtat cagcttttga tcagctgatg cactacctca
    27481 gtacaggtca agactcatct aacttacgta gcgtctttga ggcagggtct agcaacgcat
    27541 tttatagagg attgaaagag tccataaaag cgaaatttca gacagataca agaaaagcca
    27601 gccgtctcaa tattccatct atccttttga caacaatgac tcaagtgggt aaaacaactt
    27661 acgtgcttga tttcacagct acctacgaat ttttgtatga caactcaaca gatcctgagc
    27721 agcatacctc tggacatatt aatcaagact tgattggaaa agtgactgta aaaaaagttg
    27781 gacagcatta ccttatcagc cagtccggct ccaaaaatat tactgttgtt aaagaggaca
    27841 atcaactcaa agcgccatct gtctttcctg agtctatttt gggaacgtgg acaggccaag
    27901 ccaatggttt gagcatccat atgtctctag catcagatgg aacaattacg actaaagttg
    27961 aagatcaaaa aggcaaccgt tctaaagaaa ctcggacagc taaaattagt aaagttgaag
    28021 acaaaggcaa tggtttttat ctttatatgc cagatcctgg aagtgacata agcgccttag
    28081 ttccagaagg aggattgggg ggtgcaaatg tcaaatatgc ttatggtttc aaaatatctg
    28141 gtaaaacagc ctctccagtg gtgtggcagg cagcattaac acatgaattt gattatacca
    28201 agccactttc gggagtaact ttgcaaaagc aaccataaag gatgcaaagc cacaaagaac
    28261 agtagaaaca atgttatcac tcaaatacag tgctatgaaa acagataaac tcaaaaggct
    28321 aggagattat ttcctagcct tttgagcgtt taagactgcg tcaaatgtag ctgatttagc
    28381 ttgcacgcaa atgtcttctt gggttagagg gtttttcaaa cgtaattgat aagcatggag
    28441 catcagtcga gcacaatctt tttttccatt agaatacaaa ggatcaccaa ataaaacatg
    28501 accttgatgg gctaaatgga ctcgaatttg atgagtccgt ccagtttgta attgacaatt
    28561 gagtaagctt gccgttttgt ggaagttttt gactagagtt acctcagtaa tagctttctt
    28621 gccatttatc ggatcgacaa cacgttttct gcggtcatgt cgatgacgac caattggatg
    28681 atggtaagtt accctaggac tgtctaagga tccgtgaact agtgccaaat attctcgatg
    28741 gatatctcgt ttttctaaaa gacgattaag gattggtaag ataaaaggtg ttttagcaaa
    28801 taaaatggcc ccacttgttt ccttatccag acgatgaacc acatagcagg tctgtcccgt
    28861 gtaagcagac acgtggttga gcagcgccaa ctctgttgga tcattgccgt gtgttttcat
    28921 cccttcaggt ttgttaacaa taatgatgtg ctcatcttca taaaggcagg ttactttttc
    28981 ggcctgcccc atcacgataa tcttttcagg ataatcttcg tgatcaaaaa aaagtttgac
    29041 ctgatcaccg tatttaacac aactttgcca gttaacagga tgaccattaa taagaacatg
    29101 ttttttagtt ctcagaaaat gcctaatttt acggggaatt aacaattgtt cctctaataa
    29161 tgctttgacc gtcaattctt caaagggatt tttcagtgtg acagtaaacc cctttgatgg
    29221 tttatttgtt gtcttcattg agccagttat ggtatttttc aacaaggttg atagccattt
    29281 gggtggcaag agcagcagag aaagcttcag taccttcacg ctcgtcatta gctacctcca
    29341 agcgagtctc gtcgtaaatg gcttttagac tttctgcgga taaggtttct ttaaaagctt
    29401 ggaatttatt cattggttaa acccgtcctt ctatgtttaa tgtgagctcc tgattaatgc
    29461 aaggaattca atgctattat agcatgtttc taatcaaaac gtaccactca aaaaggtaaa
    29521 aacgttattt tgctaatcca tataataaga aagtgtctta aataagacct cttgatagag
    29581 tttgtaacaa aaatgatatg aaactaggtg ctaacgcaga aatagaaacc aactaacgtg
    29641 ttgcgaagtt ggaacagtca taaaaaatag caagataaaa cttatcaaca cacagttacc
    29701 taagacgatg aatcaaacag gtatgttctt tcaaaacaac cccatgaggt tgaacgacgt
    29761 atcggaaaaa tacggattag tttggcctta tgataggtat ttctctatcg taaaagaagt
    29821 caaccaaagg tttttaatat aaaatattaa gaaaaattgt tataattatg ataaaaacat
    29881 aatcgtaaaa tagaggaaaa acaatgaaaa caattagacg ttatgatgtt aacgaagata
    29941 gagggcatac aggccttgta gaagcaggag atttctatta tttaaactat tgtgtcggca
    30001 atgttggaca agacattgaa agtcagatta atggagcctt tgatgagatg gagcgccgat
    30061 tagcattggt ggggctcaca ttagatgcag ttgttcaaat ggattgtcta tttcgagacg
    30121 tttggaacat acctgtgatg gaaaaaatga ttaaagagcg ctttaatggc agatatcctg
    30181 ctcgaaaatc gattcaaacg gaatttgcac atcacggtgg accacaagga ctactctttc
    30241 aagtggatgg tgtggcttat tcaaagcata tttcgatgac ctaatcaagg ttactttaag
    30301 atcatagtgg tataataacg ttatgagatt tctagaactt ttacaaaaga aattttttcc
    30361 taaagcatat caggaaaaac aattcttaat gcatcaaaaa acgcgtttaa cgccacaaca
    30421 caatcaaaag cagtattcgc caaatgccaa tcatttggac tcatcagcta ccaaaaactc
    30481 agaacaagac cctgcaatag ctctgcaacg cagtagagcc tatgaaggaa gccctaaaag
    30541 tcggcccgct tggttgcaaa agctggaagc tgttttgccg tctcctcaac atccaattcg
    30601 gcgtttttgg cgccgctatc acatcggaaa actgctaatg attctgattg gaactcttgt
    30661 cttactctta ggatcatact tgttttactt atcaaaaaca gctaaagtat ctgatttaca
    30721 agatgccttg aaggctacaa ctgttattta tgatcacaaa ggagagtatg caggcagttt
    30781 atctggtcaa aaagggagtt atgttgagct caatgctatt tcagatgatc ttgagaatgc
    30841 tgttattgcc actgaggata ggacttttta cagtaatagc ggtattaatc ttaaacgctt
    30901 cttattggca gtagttacgg cgggccgctt tggaggtggc tcaacgatta cacagcaact
    30961 ggctaaaaat gcttatctct cacaagatca gacaattaaa cgaaaggcca gagagttttt
    31021 tttggcgtta gagttgacca aaaaatacag taaaaaagat attcttacta tgtaccttaa
    31081 caactcctat tttggaaatg gagtttgggg agttgaagat gccagtcaaa aatattttgg
    31141 aaccacagct gctaacttaa cattggatga agctgccaca ttagcaggta tgctcaaagg
    31201 acctgaaata tataaccctt accattctct aaaaaatgct actcaccgta gagatactgt
    31261 tttaggagcg atggttgatg ccaaaaagat tacccaaaca aaagctcaac aagctagagc
    31321 agtagggcta aaaaatcgct tagctgatac ttatgttggt aagacagatg actacaaata
    31381 cccatcctac tttgatgctg ttattagtga agcaatagca acttatggtc tttcagaaaa
    31441 agacattgtt aataatggat acaaagttta caccgagcta gatcaaaatt accaaactgg
    31501 catgcagacg acttttaaca acgatgaact atttcctgtt tcagcttatg acggtagctc
    31561 tgctcaagca gctagtgttg ctttagatcc taaaacagga ggtgttagag gtctgattgg
    31621 tcgtgtgaat agtagtgaaa atccgacttt cagaagtttt aactatgcga ctcaagcaaa
    31681 acgtagtccc gcatcaacaa tcaaaccact cgtggtttac gcgccagccg ttgcttcagg
    31741 atggtcaatt gaaaaagaac taccaaatac cgttcaagat ttcgatggct atcagccaca
    31801 taattatgga aattatgaat cagaagatgt tcctatgtat caagcattag caaactctta
    31861 taatattcca gcagtttcta cattgaacga tatcggaatc gataaagcct ttacctatgg
    31921 taaaacattt gggttagata tgagctctgc caaaaaagag ttgggggtag ctttaggtgg
    31981 cagcgtgaca accaatccat tggagatggc tcaggcatat gctgcctttg ccaataatgg
    32041 agtaatccat cctgcgcact tgattaaccg gattgaaaat gccaggggtg aagtgcttaa
    32101 aacctttact gataaggcta aacgtgttgt cagccagtct gttgcagata agatgacagc
    32161 catgatgcta ggtacctttt caaatggaac agcagtcaat gctaacgtat atggctatac
    32221 actagctggt aaaacaggga cgacagaaac caacttcaat cccgacttag caggcgatca
    32281 gtgggttatt ggttatacgc cagatgttgt tattagtcaa tgggtaggat ttaatcagac
    32341 cgatgaaaat cattatctaa cggattcaag tgcaggcacg gcttcagcta tttttagcac
    32401 tcaggcatct tacattttgc cttataccaa gggcagccaa tttcatgtag ataatgccta
    32461 cgctcaaaat ggtatttcag ctgtttatgg agtcaatgaa acaggtaatc aatcaggagt
    32521 tgacactcaa tctattattg atggtttaag aaaatcagca caagaagctt cgcaatcact
    32581 atcaaaagca gtcgatcagt cagggttacg tgataaagcc caatctattt ggaaagagat
    32641 tgttgactat tttagatagc ttgcagtatt ttagagatcg tgttaaaata atagttacgg
    32701 aggcgttatg gcacagaaaa aagcgagtct agcgtgtgtc gaatgtggca gtcgcaacta
    32761 ctctatcgga gtgagtagca cgcctaaacc aacacgtcta gaagtaaata aattttgcaa
    32821 atactgcaaa acatatacct tacacaaaga aacacgttaa aaaggagagc ctgatgggat
    32881 tcattagcgg gacttttaaa gtcttaaaag acacaacatg gccaaatcgt aaacaacgtt
    32941 ggaaagattt tatctcagtt cttgagtaca cagctttctt tacggtgatt atctacatct
    33001 ttgaccagct cttagcaaag agtgtattag cactgattaa tttattttag gtcaagattg
    33061 gtagaaatga taagcttgct tggaaaagcc taggctggga gtattcccag ccttttttgt
    33121 agtatcaata atctgattag taagcttttt taaagcccat actggtttga gctattcaaa
    33181 agcataaagg taaaggcaat gtagcaacga tctccaaacc aagttcacta aattggcttg
    33241 taacagagtc atcgcataaa cagcctggcc tcacttgctt agcacattaa aaatagcaat
    33301 gaatattatg gttgaggaaa acagcctgat agtctgctaa atcttcggga tgataggcct
    33361 tgacctcagc catctcatac tccctaccta gaagtttgta cttgttttct ccaaattgat
    33421 ggaaaggtaa cagttgcact tggtcaatct caagctgatt aaagagctct gagaaggctt
    33481 tagcatcgtc tagggaatca ttaaattggg gaataacagg gatgcgcaag acgatttctt
    33541 ttccagcttg aaaggcataa tgaatatttt tgataatcaa gtcattacga accccagtca
    33601 ctttttggtg tctgagctga ttataatgtt tcaaatcggt atagataaag tccacgtagt
    33661 caataagggt gacaaattgc tcgtgtttgg caaaggcggt ggtttcaatt gcggtgtgaa
    33721 gacctgctgc tttggctgct tttaacaggg ctaaggcaaa gtcaaactga gcaaagattt
    33781 ccccccctga cagggtcatg cccccacctg actcttcgta gaaatcaagg tctttaagga
    33841 cttcttcgat cacctcgtcc acggtttttt cctctccaac tattttagta ttaagcccat
    33901 cacttgttaa catctgctca ggcgcttttt gttgggattc gggattggca caccaaggac
    33961 atctcaaggg gcaacctttt aaaaaaacgg ttgtccgaat accagggccg tcatgaatac
    34021 taaaatgttg gatgttgaaa acaataccgc gatcagtcat aagtgtgaat tcctttcgtt
    34081 gtaaaaccat cataccagat aaacgtaaat aaatcaatta cgaatgaaag taaatatccc
    34141 ttttctttta agaaaataac tggtacaatg aaagagaggt gaatgatgaa tcgattagaa
    34201 cgtattattc aactggtttc ccagaagaaa aaaatagatg ttaacagttt atcagagcaa
    34261 ttagacgttt cgaaagtaac cattcgaaaa gaccttgata agcttgaaag taagggcttg
    34321 ttgcgacggg aacatggcta tgcggtgcta aacagtggcg atgacctcaa tgtacgcttg
    34381 tcctacaatt acaacataaa gcgacggatt gcagaaaaag cagctgaatt ggtgcaagac
    34441 aacgatacca ttatgattga atctggctca acctgtgccc ttttggcaga agtgctttgc
    34501 cagaccaaac gaaatattaa aatcattacc aattcgtgct tcattgccaa ctacattcgt
    34561 caatatagta gctgtcaaat cattttactg ggcggttatt accagcccaa ttcagaggtg
    34621 actgttggtc ctttattgaa agaaatgata tccctttttc atgtcaatcg tgtctttgtt
    34681 ggaacagacg gtttcaacaa agatcttggc tttatgggca aggacatgat gcgctcagaa
    34741 ggggtgcgtt acatggcaga tgctgctgag gaagtggtga ttctgaccga ttccagcaag
    34801 ttttcaaaaa ccagtctggt acaccagttg tcattagctg atgtcaatcg cgtcattaca
    34861 gaccaagcac tagacaagca aacacaagag ttattaagtg ctagtggtct tgttcttgat
    34921 tttgtttctt agaaaatagg tggctagatg aaagaagaac gcagacggct ccttgccaag
    34981 gtggcttact tgcattatgt tcaaggcaag agccaaacct tgatttcaaa ggaaatgaat
    35041 atctaccgaa caacggtttg tcggatgtta gcaaaagcca aggaagaagg cattgttcgg
    35101 attgaaattg ccgactatga tgctgattta tttgctttag aagaatacgt gcgacagcag
    35161 tatggcttgg aaaaactaga ccttgtgccc aaccaggttg aagatacccc aatggatacc
    35221 ttgacaaatg ttgctaaaac agcagctgag gtttttagac atgttgttaa agatggcgat
    35281 aagatcggtc tgtcctgggg agcgactctt tcttgcttga tggatgagtt aaatccaaaa
    35341 gcgatgaaag atgtgtttat ttatcctttg gcaggaggac ctagccatat taatgccaaa
    35401 taccatgtca ataccctggt ttaccgcttg gctcgcatct ttcatggtaa tagcgctttt
    35461 atgaacgcta tggtcattca agaagacaaa cacttggcta agggaatctt acaatctaaa
    35521 tactttaacg acattttaac aagctgggac cagttagatc ttgccttagt aggaattggt
    35581 ggggagccta atagccttga gcagagccaa tggcgtgatt tattaaccag ctcagatcat
    35641 gaccaactca aatatgaaaa agccgttggt gaagtttgtt gccgtttctt tgatcaggca
    35701 ggtcagccag tttacactgg cctacaggat cgaactattg gtatttcact agaacagttg
    35761 aggcgtgtcc ccaaaacaat ggctgttgca acaggtaaac acaaggctaa ggcaatattg
    35821 gcagccttaa aagcaggttt tatcaattat ctagtgacgg ataaagagac catgctagct
    35881 gttttagcgt tagatgaaga tattgatctt aataacgttc ttctttaata gaaattcaaa
    35941 aatactatga tatttataga ggcccaagac caaacggtca gggctttttt tgtttgatgt
    36001 gttgataaaa cagagattgc tcaaatgtgc agttaatgta ttggcacaaa agtgcattaa
    36061 ccaaaatata gcacaaaata tattgaccct aaaaacaaaa atgatacaat aaacttacca
    36121 acgaaatgta aacgattaca aacacaataa catcaccttc gaaaaaaaga ctcattgccg
    36181 ttacatcaca tgaaaaagaa aaaaaggaga caccaatgca agttattgta ccagaccaaa
    36241 tcattatggg cttaatttta aatgcaggcg atgccaaaca acatatctac caagctctga
    36301 aatgtgctaa agaggacgat tatgccacta gtgaaaaaga aatggcctta gcagatgatg
    36361 ccttattaga agcccacaat ttacagaccc agtttcttgc ccaagaagct agtggtaata
    36421 agtcagagat tacagctctt tttgttcact cacaggatca cttgatgacg accattacag
    36481 aaattaatct catcaaagag attattgacc ttcgtaaaga attggcaact aaataagttt
    36541 tcatgactaa aaaggagatt gacatgatta aaattggatt gttttgtgca gcaggatttt
    36601 caaccggtat gttggtgaac aacatgaaag tagctgctga aaaaaaaggc attgattgtc
    36661 agattgaagc ctatgctcaa gggaaattag cggactatgc accattactt gatgtggcac
    36721 ttttaggccc acaggttgcc tatactctgg ataaatcaga agctatttgt aaagacaatg
    36781 atatccctat tgcagttatc ccaatggctg attatggaat gttagacggc aacaaggtgc
    36841 ttgatttagc ccttagcctt gttaaagaat aggagtcaat catggcaaaa atgaatatgc
    36901 aaaacatcat caagccaatc atgacatttg tgaatatgcg cggcatcatt gccttaaaag
    36961 acggtatgtt agccatttta cctttgaccg ttgtggggag tcttttcctg attgctgggc
    37021 agattccttt ccaaggggtt aatgatgcca ttgctagtgt gtttggtgct gactggacag
    37081 aaccctttat gcaggtttac catggaacat ttgctattat ggggttgatt tcctgttttg
    37141 caattggcta ttcttatgct aaaaactcag atgttgaacc tctcccttca ggggttctat
    37201 ccttgtcagc cttctttatt ttattgagat catcttatgt tccagcagaa ggtgaagcta
    37261 ttggagatgc tatcagtaag gtttggtttg gtggtcaagg gattattggt gctattgtga
    37321 ttggtttaac ggtcggagct gtttacacaa catttatccg tcgtcatatc gtcattaaaa
    37381 tgccagatca agtgccacaa gccatcgcca aacagtttga agccatgatt ccagcctttg
    37441 tgatctttac cttgtcaatg cttgtgtata ttattgccaa gtcagtaaca ggtggtggca
    37501 cctttattga aatgatttat gatgtcattc aggtaccgct gcaagggtta acgggttctc
    37561 tttatggcgc actaggtatt gccttcttta tttcctttct ttggtggttt ggcgtgcatg
    37621 ggcaatctgt ggtcaatgga attgtcactg ctcttctctt atcaaatcta gatgccaata
    37681 aggccttgat ggcagcaggt gagttatcct tagacaaggg tgcccatatt gtaacccaac
    37741 aatttttaga ctctttcttg attctatcag gttctggcat tacctttggt ttagtggtag
    37801 ctatgatctt tgcggctaaa tccaaacaat acaaggcttt aggtaaggtt gcagccttcc
    37861 cagccctctt taatgttaat gaaccagttg tctttggttt tccaattgtg atgaatccag
    37921 tcatgttctt gccatttatc ttggttcctg tcttggcagc tctcacagtt tatggcgcca
    37981 ttgctattgg tttcatgcag ccctttgcag gagtgaccct tccgtggtca acaccggcca
    38041 ttgtctcagg gttcatggtt ggtggctggc aaggagctat tgtgcaaatt cttatactca
    38101 tcatgtcaac gttggtgtac ttcccattct ttaaaattca agataatatg gcttatcaaa
    38161 atgaacaagc tagtgaagag tcatgattta gcgtccacac aaattcttga gaccatctcc
    38221 agttagaggt ggttttttag aaaaagaaga gcgacttatc aggcctatat tgcacaatag
    38281 tgcaaaataa agtgactatc tcttcttatg gttaaggata cgcaaaagtc gcaatatatg
    38341 ataaaatagt tacgaaagaa acatttataa tttcgtaaga aagaaggaga ccccgcatga
    38401 ctgaaacaaa aagcccttat tttggacatt taacagacag aatgactcac tatcgtgaag
    38461 ctgtgttaga caagaaacct tatattgatg ctgagcgtgc tattttagcc actgaagcct
    38521 atcaaaaaca ccaaaataag ccagcaaacc tcaaacgcgc ttacatgctt caaactattt
    38581 tggaaaacat gaccatctat attgaggatg aaagcctcat tgctggtaac caagcctctt
    38641 cgaacaagga tgcgccgatt ttcccagaat acactctgga atttgccctc aacgaacttg
    38701 atctttttga aaaacgagat ggggatgtct tctacattac cgaagaaacc aagcaacagc
    38761 tccgagacat tgccccattc tgggaaaaca ataacttacg tgctcgctgc ggggtcttgc
    38821 taccagaaga agtgcaagtg tacatggaaa caggtttctt tggcatggaa ggcaagatga
    38881 actctgggga tgcccattta gcggttaatt accaaaaact cttggagcac ggcttaaagg
    38941 gatttgaaga acgggcacga gctgccaaag ctgcgcttga tttgaccatt cctgaaaaca
    39001 ttgacaaata ccatttttac gactctgttt ttattgtcat tgatgcagtc aaaacttatg
    39061 ccaaacgcta tgccaagttg gctagagaac tagcaaaaac ggctaaacca gaacgtcaag
    39121 cagaattgtt agacatcgcg cgtatttgtg acaaagtgcc ttacgaacca gcgaaaacct
    39181 ttgcagaagc tgtgcaatct gtctggttca ttcagtgtat cttgcaaatt gaatcaaacg
    39241 ggcattccct atcttatggc cgctttgacc agtacatgta tccttatgtc aaagctgatt
    39301 tggaggctgg ccgtgaaaca gaagactcaa tcgttgaacg tttgactaac ctctggatta
    39361 agaccttgac cattaacaaa gtgcggagcc aagcccacac cttctcatca gcaggtagtc
    39421 cactttacca aaacgtgacg atcggtggtc aaacacgcga taaaaaagat gcggtcaacc
    39481 cactgtctta ccttgtccta cggagtgtgg ctcaaaccaa attgccacaa cctaacttga
    39541 ctgttcgtta ccacaaaggc ctagacaata cctttatgaa cgagtgtatc gaggtcatga
    39601 aacttggttt tggcatgcca gccatgaaca acgatgaaat catcattcca tctttcatca
    39661 aaaaaggtgt ctcagaagaa gatgcttatg attactcagc cattggctgt gtggaaacag
    39721 cggttccagg caaatggggc taccgttgca cggggatgag ttacatcaac ttccctaaaa
    39781 tcttgctgat taccatgaat gacgggattg acccagcatc aggcaagcgc tttgcaaaag
    39841 gatacggtca cttcaaagac atgacctctt atgaagaatt aaaagcagca tgggatgcca
    39901 ccttgcgcga aattacccgc atgagcgtca ttgttgaaaa tgccattgac ctaggtcttg
    39961 aacgtgaagt gccagatatt ctttgctcag ccttgacaga tgattgtatc ggtcgtggca
    40021 agaccttaaa agaaggcgga gctgtttacg actatatctc aggccttcaa gttggaattg
    40081 ccaacctatc agactcccta gcagcgctga aaaaattagt gtttgaagaa ggccgcttga
    40141 ccccagaaga actctggcag gcccttgaaa gtgactttgc tggagaacgc ggagaagaaa
    40201 tccgccaaat gctcatcaat gacgcaccaa aatatggtaa cgatgatgac tacgcagata
    40261 gtttagttgt cgaagcttac gacacctaca ttgatgaaat tgccaaatac ccaaataccc
    40321 ggtatggccg tggcccaatc ggaggtatcc gctattctgg gacatcatca atctcagcca
    40381 atgtcggaca aggaaaagga accttagcga caccagacgg tcgccatgca ggcacaccac
    40441 tagcagaagg ctgttcacca gaacacagca tggacaaaaa aggacctact tcagtattga
    40501 aatctgttgc taaattacca acagatgaga ttgtcggagg agtactactc aaccaaaaag
    40561 ttaacccaca aaccctggcc aaagaagaag acaaactgaa attaatggcc ttacttcgta
    40621 ccttctttaa tcgcctacac ggttatcata tccagtacaa tgtcgtgtca cgtgaaacct
    40681 tgattgatgc ccaaaaacat cctgaaaagc accgcgacct cattgtccgt gttgccggct
    40741 actcagcttt cttcaatgtg ctctcaaaag caacccaaga tgacattatt gaacgtacag
    40801 aacacacact ttaggagaaa ataagtatgg aatacatgtt agacacttta gatttagaag
    40861 ctattaaaaa atggcatcac attttgcctc tcgcaggtgt gacctcaaac ccttctattg
    40921 ccaaaaaaga aggtgacatt gatttctttg aacggattcg agaagtccgt gctattattg
    40981 gtgacaaggc ttctattcat gttcaagtga tcgcccaaga ttacgaaggc atcttaaaag
    41041 atgctgctga aatccgcagg caatgtggag atagtgtgta tgtcaaagta cctgtaacca
    41101 cagaaggcct tgcagccatt aaaaccttga aagccgaagg ctatcatatc acagcaaccg
    41161 ctatttatac gaccttccaa gggttattag ccattgaagc tggcgctgat tatttggctc
    41221 cttactataa ccgtatggaa aatctcaaca ttgatccaga agctgtcata ggacaattag
    41281 cagaagctat taaccgtgag aatgctaata gtaagatttt ggcagcaagc tttaaaaatg
    41341 tggctcaggt caataaatcc tttgctctag gagcacaagc catcactgct ggcccagatg
    41401 tgtttgaagc aggatttgcc atgccatcta ttcaaaaagc ggttgacgat tttggcaagg
    41461 attgggaagc cattcatcac cgcaagagca tttaagaaat agcatcagta gtaatcacat
    41521 cattaggaat cacagataaa ggagttcaac gatgaaagta tttgcaagtc catctcgtta
    41581 cattcaaggt aaaaatgcct tgtttaccaa tgctaaaacc ctaaaacaac taggggatag
    41641 ccccatttta ctgtgtgatg acgtggtgta tggcatcgtc ggagaaagat ttgaaagcta
    41701 tttgatagat aatggcatga ctcctgttca cgtagccttt aatggcgaag cctcagataa
    41761 tgaaatcagc cgcgtggttg ccattgccaa ggaaaatggc aatgatgtta ttattggact
    41821 tggtggcgga aaaaccattg acagtgctaa ggctatcgct gacttacttg ctgttccggt
    41881 gattattgcc ccaaccattg cttcaacaga tgccccaacc tcagccttat cggttattta
    41941 caccgatgaa ggagcctttg aaaaatacat tttctattca aaaaatccag atcttgtttt
    42001 ggttgacaca caagtgatct gtcaggcacc aaaacgatta ctggcctctg ggattgcaga
    42061 tgggttagca acatgggtag aggcgcgtgc tgttatgcaa aaaaatggag acaccatggc
    42121 aggtggcaat caaaccttgg caggagttgc cattgccaaa gcctgtgagc agaccttgtt
    42181 tgcagatggc ctaaaagcga tggctagctg tgacagacaa gtcgtgaccc cggccttgga
    42241 aaatgtcatt gaagccaata cactccttag tgggcttggt tttgaaagtg cgggtctggc
    42301 tgctgcacat gccattcaca atggctttac agcgctgaca ggtgctattc atcaccttac
    42361 ccatggtgaa aaagtcgcct atggcacgtt gacccaactc ttcttggaaa atcgttcgcg
    42421 tgaagaaatt gaccgctaca tcgactttta ccaagctatt ggcatgccaa caaccctcaa
    42481 agaaatgcat ttagacacgg ctactcaaga ggatttctta aaaattggcc gtcaagcaac
    42541 catgacaggc gaaaccatcc atcaaatgcc atttgttatc agtccagaag atgtagcagc
    42601 ggctttggtt gctgttgatg cctatgttac gagtcgatag gctaaccaaa aagagatttc
    42661 ttatttgcca gataacctca aaaaaacaag tctagggagt cacttcctag atttgttttg
    42721 cttattgttg gttggtaata gcacttttag cggggtcatt gtgggcaata cggctggcag
    42781 ctgcagcagc aatggcgcta acaatatcat ctaaaaaggt atggcaggag tagccatctt
    42841 tatggttgag tttgtcaaca attcctggtt ttgtcttgtc cagataccca taattggtaa
    42901 aaccgataga cccatagagg ttaacaatgg ataaggccag gatttcatca atcccataca
    42961 gcccttggtc ggttttcaag atactaagga gtggctcaga cagctggttg gcttcagcca
    43021 atttatctaa ttctacccca gtgataatag cgttttgcac ttctcgtttt gcaagcaccg
    43081 cctcaacgct ctctaaacat tctgccatgg tcaaattggg aatataatca ttctgcaaaa
    43141 aaagcaccag ctctgcaata gcttctaaag aaccccctcg ctcagccagt aattgataag
    43201 acacttctcg taggtgatgg tcagttgaca tgtgagacct cctttttttc tttattgtaa
    43261 cataaaagat ttcgagaaac ctctacaaca gctaaagagg ggatagttct tttttaagag
    43321 atgttgacta ctgagatccc ctacttggta aaagtcaaag aaacgatagt aaagtaacca
    43381 tcttacctag gcctaaatac aatcaccacc cctaggtgac acacagcacc tatttcaaaa
    43441 atgatattag tatggcaaaa caaaaagaca acgccttctt ttttctcctt actatctcct
    43501 ttaattttca tattttttaa aaaaactatt gataaactag ttaagtaagc gtatactatg
    43561 gttagtgagc gaaattagaa aagaggacaa gcatatgaat ctacttggat caagacgggt
    43621 tttttctaaa aaatgtcggc tagtaaaatt ttcaatggta gctcttgtat cagccacaat
    43681 ggctgtaaca acagtcacac ttgaaaatac tgcactggca cgacaaacac aggtctcaaa
    43741 tgatgttgtt ctaaatgatg gcgcaagcaa gtacctaaac gaagcattag cttggacatt
    43801 caatgacagt cccaactatt acaaaacctt aggtactagt cagatcactc cagcactctt
    43861 tcctaaagca ggagatattc tctatagcaa attagatgag ttaggaagga cgcgtactgc
    43921 tagaggtaca ttgacttatg ccaatgttga aggtagctac ggtgttagac aatctttcgg
    43981 taaaaatcaa aaccccgcag gctggactgg aaaccctaat catgtcaaat ataaaattga
    44041 atggttaaat ggtctatctt atgtcggaga tttctggaat agaagtcatc tcattgcaga
    44101 tagtctcggt ggagatgcac tcagagtcaa tgccgttaca ggaacacgta cccaaaatgt
    44161 aggaggtcgt gaccaaaaag gcggcatgcg ctataccgaa caaagagctc aagaatggtt
    44221 agaagcaaat cgtgatggct atctttatta tgaagccgct ccaatctaca acgcagacga
    44281 gttgattcca agagctgtcg tggtatcaat gcaatcttct gataatacca tcaacgagaa
    44341 agtattagtt tacaacacag ctaatggcta caccattaac taccataacg gtacacctac
    44401 tcagaaataa taccaaaagg ctagacctct gctcactagg cctagctttt tacatcaaaa
    44461 aaagcaatga ctatagaaag taaaaatact agaaaaagca atgattgccg tcattgcttt
    44521 ttatgatttg tcaaaaagta aaaagcaaat tgaaaaaatc gccctggagc tgttgagata
    44581 aactacacca tttattaaga taccaaatca aaaagctaac accataagag cgttagcttt
    44641 ttaaagagcc tcaggacagt ttatgtttaa tggcttctag gtaggtttga aacatatgat
    44701 ggatcgtttt gcaattaagt aattggaaga tctccaagat ctgcacacac ttttgctcag
    44761 ccccctcttt accatttatg aggccaataa tgccatcaaa aaaagacaca caacaccgtt
    44821 catataagca atcatcattt agctgataat cttcaatttt tgccaaaatc tcagaagctt
    44881 tatcatactc ttgtcgctga ataaacaaaa tcaacatatt gacaaacatc cgaatcgatt
    44941 cattcccata ataccttagg gtattgtatt tatcgaggtt caaaataact tttgaaaaca
    45001 ccatctcaat aaagcaagac tcaaaaataa acatacaatt attaaaaagc acagtctcat
    45061 agtgactcca agtttcaata ttaataagat agtttgacaa ataagtccgc tctgtcagac
    45121 agtcttcctc agtaagagtt gctaataaca ccttgaccaa ggcaaaaagg ttcttttcct
    45181 ttgttgactt actatcttta taagaatcaa ggatatgatt taaaccttct atgtttcggc
    45241 attcaaaata atgttttgcc gtatccatat caataaattc tttgtattgt ttaaagttat
    45301 tactaataaa gagaaactca tcaacattga cattcaagtt gtctaagata taaagtaact
    45361 ttttcgaaga aatgtcaaga ttatttttaa taaaacgata ataggtttgc ctagtgagat
    45421 aatcaccaca aacttgttta atcgaaatgt tttttgaatg cctaatgaat tcaacggttt
    45481 caccaatttc catatgtcaa gccttcctag ttgatgtcaa aaatacgtta cgcatgtacc
    45541 taatacgtaa caagttgaat gtttcggatg atagtcgctt atgataggtg cataaggtca
    45601 atagccagat gcgatagttt tatcaactgt catatgttaa acctttctaa tctaatagat
    45661 gttaaaaata cgttacgtgt gtacctaata cgtaacaaaa taatgggtta gcaaaataag
    45721 cagctatgat atagccataa ggttaaaagg aggcgcctac tatgtggtta ttgttactat
    45781 ttttgtagtt ccttttgcaa aatggtagta gataaaaatg agaagtcaat aaaagcattg
    45841 acaaacaaaa gaatcatttt gtttatatta tcagtatttt taagtttctt gtcaactgaa
    45901 atgagcatct actagccaca atagtaacta aaaggctaat tgtcagtaaa aatgagataa
    45961 atcagccagg aaaaagccta cgagtcaact aatggacaac ccaaaaaaag acaaaggact
    46021 ttcacttggt gttgtggacc ctatcacgat ccttatcaca gaatcagtta ttaacaaaat
    46081 gagaacggtc ccatatcatc taaaaaaact gtcaagcaca acctattgtt tgctagctga
    46141 tcttgcctat cagtctgcct agtagatacg gcagaagata agagatcaac caaaagatga
    46201 taagtgcaac gactcatagc gtcttttgac caaaaccaat taggctcaca tgagatgaga
    46261 ataattgggt tgggttgtca gtgtcaacta accgtgttat tgtctattac cattcatggt
    46321 atcagcgaca tcgtatgata accatacgat tcagctaagt aaggaggtgt gtccaatgta
    46381 ccgttaaaag caaatgcagt agattaactt attttgaaag aggtataaaa aaaatgaata
    46441 aaaagaaatt aggtatcaga ttattaagtc ttttagcatt aggtggattt gttcttgcta
    46501 acccagtatt tgccgatcaa aactttgctc gtaacgaaaa agaagcaaaa gatagcgcta
    46561 tcacatttat ccaaaaatca gcagctatca aagcaggtgc acgaagcgca gaagatatta
    46621 agcttgacaa agttaaccta ggtggagaac tttctggctc taatatgtat gtttacaata
    46681 tttctactgg aggatttgtt atcgtttcag gagataaacg ttctccagaa attctaggat
    46741 actctaccag cggatcattt gacgctaacg gtaaagaaaa cattgcttcc ttcatggaaa
    46801 gttatgtcga acaaatcaaa gaaaacaaaa aattagacac tacttatgct ggtaccgctg
    46861 agattaaaca accagttgtt aaatctctcc ttgattcaaa aggcattcat tacaatcaag
    46921 gtaaccctta caacctattg acacctgtta ttgaaaaagt aaaaccaggt gaacaatctt
    46981 ttgtaggtca acatgcagct acaggatgtg ttgctactgc aactgctcaa attatgaaat
    47041 atcataatta ccctaacaaa gggttgaaag actacactta cacactaagc tcaaataacc
    47101 catatttcaa ccatcctaag aacttgtttg cagctatctc tactagacaa tacaactgga
    47161 acaacatcct acctacttat agcggaagag aatctaacgt tcaaaaaatg gcgatttcag
    47221 aattgatggc tgatgttggt atttcagtag acatggatta tggtccatct agtggttctg
    47281 caggtagctc tcgtgttcaa agagccttga aagaaaactt tggctacaac caatctgttc
    47341 accaaatcaa ccgtagcgac tttagcaaac aagattggga agcacaaatt gacaaagaat
    47401 tatctcaaaa ccaaccagta tactaccaag gcgtcggtaa agtaggcgga catgcctttg
    47461 ttatcgatgg tgctgacgga cgtaacttct accatgttaa ctggggttgg ggtggagtct
    47521 ctgacggctt cttccgtctt gacgcactaa acccttcagc tcttggtact ggtggcggcg
    47581 caggcggctt caacggttac caaagtgctg ttgtaggcat caaaccttag tatggaaatg
    47641 catttcgtta gaacagaacc tgaggcacgc cgcatagctg aaaccttttg tgctgaaaac
    47701 acacaaacaa aaacccctat gcgcgtgcag cagttaagct atccatcaga cacagatcac
    47761 tcaggtggtg agctatacat ctatgctttg tctcctgctg gatttatcat cgtatcagga
    47821 gacaccagag cgcacaccat tttaggctat tcttttgata ataacctaga cctcaaccat
    47881 gataatgtca ggagtatgat agaagcttac caaacacaaa tcagctcttt ggattgatcg
    47941 gtcaaagtag cttttgcccc ctcaccactc gtttaagacg accgtgtttc taaaatacca
    48001 ctacctttta agtttcacgc cggcctaagc gagtgtttgt ctcatggagc aaactgtttc
    48061 catcgaaaag tcacccattg ataaaggagg tcatcaacgc ctactaatga tgacttccct
    48121 aagatgccaa ggtaaaggac tgagttctgt agagatggga ctcagtcctt ttacgatttt
    48181 tgggagttat tctagccata ccgtggcaag aaaacaacac tcaaaaatag ccaacgtcag
    48241 tttctgttca tacatgacca cagccaaaca ccacaaccta acctaaccta tcctaaacac
    48301 agtggctttc taacgaacag tcacaacgag cagcaacacc aaaaagcaag attaagctac
    48361 gtttaagact aatgatataa gataacagta attattaatg agaaaagagg ctcaaaagga
    48421 catgaaacaa atgaataaac tcattacagg agtggtaacg ctggcgacag tcgtgacctt
    48481 atcagcttgt caatcatcac acaacaacac caaactcgtc tcgatgaaag gagacaccat
    48541 cactgtcagt gacttctaca atgagaccaa aaacacagaa ctcgcacaaa aagccatgtt
    48601 aagcttggtg attagccgcg tttttgagac acaatatgcc aacaaagtct ctgacaaaga
    48661 ggttgaaaaa gcctataaac aaaccgcaga ccaatacggt acatccttta agacagtcct
    48721 agcacaatca ggcttaacac cagaaaccta taaaaaacaa attcgcctca caaaattagt
    48781 cgaatatgcc gtcaaagaac aagccaaaaa cgaaaccatc tcaaaaaaag actaccgtca
    48841 ggcctatgac gcttataccc caaccatgac cgcagaaatc atgcagtttg aaaaagaaga
    48901 ggatgccaaa gcagcacttg aagccgtcaa agctgaaggg gcagactttg cagctattgc
    48961 caaagaaaaa accactgcag ccgataaaaa aacaacctac acgtttgact caggtgaaac
    49021 aaccctacca gcagaagtag ttagagctgc atcaggcctc aaagaaggga acagatcaga
    49081 aatcatcaca gcgcttgatc cagccacctc aaaacgcacc taccatatca tcaaagtcac
    49141 caaaaaagca actaaaaaag cagactggaa agcgtaccaa aaacgcttga aagacatcat
    49201 cgtgactggc aaattaaaag accctgactt ccaaaacaaa gtcatcgcta aagctcttga
    49261 taaagcaaat gtcaaaatca aagacaaagc atttgccaat atcttagccc agtttgcaaa
    49321 acctaaccaa aaacaacctg ccccaaaata gagggttgtc agtttatttc cctaaggttg
    49381 cttaaaaaga tagccctagc catcactccc cttttcaaaa caccgataat ggaaacgtgt
    49441 cctatactga tggtctaggg caatgtcata aagctaaaga ctctatccaa aaataaggat
    49501 agagtcttta atgttttaaa accagtcagc aaaaaaattc acgctaagcc tagctagaat
    49561 gaggcttagc gtgaatggta tcagtgatag tccgttaaac tagctgattg ggagtcagaa
    49621 actctcctta cccaatctga ctataccccc agtaccagag gtgtctcatc gcagaagtag
    49681 aaatcagata atcgtctgat aagctcttgt cctcaagcaa ctgatgagta tcgtcgcacg
    49741 ggccatgagc tgtcggtggc tgatagctag taggctcatc agccaaaact tttttggagt
    49801 aacgctttgg tctcagacgg cgccagaggg ttgagagagg ggtgagaaag aaacgatggg
    49861 tcatcttggc ctccttatct aatgatagtc accttaacag acaagagtgt caatactgtc
    49921 attttaagga gaaaaagatt aaaaatcaag gtcacaaact ttcctttgct gaaaaaacgc
    49981 gaccataata aagaacctaa atacccctcg tacttgatct tctctttgcg cagttatctt
    50041 gcgcactcag tttcctccaa gatagtgata gcgataccat tactgaccta taggctatcg
    50101 cggcaacccc ttttttcgcc tcaatgaggc tgatacagct gtcatgacag gaaaaagacc
    50161 tctgcctatc taagggatca ttaatttttt tctcaccaaa aaactgattt tagaaacgaa
    50221 aaagcatggt gtataataag gttcggaaca attatgacat aataatgaaa gtaaggttaa
    50281 cgaaacattg acaaattgta agtataaact tagaaagcta tctgtagggc tcgtctccgt
    50341 cggaacgatg ctgatagccc cgacagtttt aggacaggag gttagtgtta gtactgagac
    50401 gagtgctagt actagtacta gtaccgctag cgctggtacc agtacgagtg agacagctgc
    50461 cagcggaact gcgagtggaa ctaatggtgc gtcttccgta acatctagtg aaggaagtca
    50521 gagttcagaa tctgccccag cctcacctca acctcaagca cagacagctg tagctagttc
    50581 ttcgagctcg tctaatgcga atgcctcttc tagtagtgac gggaaagctc ctcaggcagc
    50641 aagcactacc tcatcttcaa ccccagcagc agccagtagt aatggtatca gtcaaggaac
    50701 tagtgctgaa gctgaaactc caatgatgga ggtggaacag tatacagttg gtaataaaac
    50761 aacagaacta aatattaaag acggtgctca gctaaaaaac gggagtaagg ttgataaaga
    50821 tacaaaactt atcagaaacc gtgatggtga gcagcgtgat attgttgata tcaagcgtga
    50881 ggtaaaaaca aacgcagatg gcactgtcga tgttacggtt accgtaacgc ctaaagaaat
    50941 tgacaaaggt gccgatgtta tggcccttct agatgtctct aaaaagatga cagaagatga
    51001 ttttaacaac gctaaggata agatcaagaa attagtcaca accttaacga gtaaatctcc
    51061 tgacagtcaa ccaaaccata atgctcgcaa ctctgtgcgc ctaatgacct tttaccgtaa
    51121 ggttaacgat ccgattgagc tcaacgcaga taatgtagac gctaaactag atgaggtttg
    51181 gaaaaaagct aaagaagatt gggactgggg cgttgattta cagggagcta ttcacaaagc
    51241 tcgagagatt tttaataaag gaaaaagaaa aaaatctggt aaacgccagc atatcgtctt
    51301 gttctctcaa ggtgagtcga cttttagtta tgacatcaac gacaaagatg aaaatgatac
    51361 tgtccagaaa aatagaatag ctggaaaagt tacgacctct aacccactat ttccctggct
    51421 acctatattt aatcatacaa atcaaaaagc agaagttatt gatgatgtag acaaattact
    51481 gggtttagct gaaaaaatgg gaatatcact gcccgatgga ttaagagcag gagtgaaggc
    51541 tattggggca ggtagtagtt tccttagtac cttcacagga tctggattga cagagtacct
    51601 cactttgaat gaatatcgct cagatatact aaaagaaaaa cagttcgatt ataccaagcg
    51661 tgtaggtgag ggctactact accacagtta ttcaaagcga actcatggag ataagatgcc
    51721 ctttgagaaa caaataagag aagttttaga gagattttta ccgaaaatcg aagataaaga
    51781 atggcctaaa aagtttatag aaatttttgg attacaagaa aaagaagtaa atcaaattgg
    51841 ggtagatgta attatgaaag taattaacag catcttctat aagcgtgaat accattacta
    51901 taatcacaat ctttcagcca ttgctgaagc caaaatggcg cagaaagagg gcattacctt
    51961 ttattccgtt gatgttactg actctgataa ggcttccaaa agggtaaagc gacaagttgg
    52021 aaatgagcag tctaaaaaga aaaaagaaga tgctgaaaaa gatcgaaaca ataagtttga
    52081 cgactatctg aaaaagatgt ctgaaggtgg taatttctta agtaaggttg aggagagagg
    52141 taagttcaaa gacactttaa cagagctaac gattaaagat gaatttacaa ataaggttaa
    52201 ggttcaggaa gattcagaag gtaaaaaata taaaaccggc ttaattagtg gaacatcaaa
    52261 tgtgacacat acccctgcta aaaccggatt tttctcatcc aatgaaagcc ttacttggac
    52321 catttccaaa gagcagttga aaaaagcctt tgaagatgga acgtctttaa ccctcaccta
    52381 taagcttaaa gttgagaacg acaagtttaa aaaatctctc gaggaagata ataagaagaa
    52441 gagagcaaaa cgttctgcac ctacagaaaa tgaaaactct gtcacagaaa aaatcatttc
    52501 aaatactact acctacaaaa ttaatgaaca aaaagttgaa ggtaacaaat ttgatgacgt
    52561 taatctgaca tacagtaaat tcaaggttcc tgtaccacag attgatggcc aagttattga
    52621 gccacaagca cctaaattac ctgacttacc tcctgttatt gagcatggcc ctgttcttga
    52681 ttacacagaa gaatcaatat accgattacc acttgaacat ggcagcaatg caccagacac
    52741 acaagtgaca attgaagaag acacagttcc tcaacgccca gatatccttg taggcggtca
    52801 gagtggaccg gttgacatca ccgaagatac ccaaccaggt atgtcaggct caaatgacgc
    52861 gacagttgtc gaggaagaca cagcacctca acgcccagat atccttgttg gcggtcaaag
    52921 tgatccaatc gatattaccg aagataccca accaggtatg tcaggctcaa atgacgcgac
    52981 agttgtcgag gaagacacag cacctcaacg cccagatatc cttgttggcg gtcaaagtga
    53041 tccaatcgat attaccgaag atacccaacc aggcatgtca ggctcaaatg acgctactgt
    53101 tatcgaagaa gatacgaaac caaaacgctt cttccacttt gataacgagc cacaagcacc
    53161 agaaaaacct aaagagcaac catctctcag cttaccacaa gctccagtct ataaggcagc
    53221 tcatcacttg cctgcatctg gagacaaacg tgaagcatcc tttacaattg ttgctctaac
    53281 aattattgga gctgcaggtt tgctcagcaa aaaacgtcgc gacaccgaag aaaactaacc
    53341 ctcgttagtc tagcgactct aagcctttgg ctttaaaaat ttctcattat ccccctcaaa
    53401 caccggttcc tcactgagat ggcgctttaa aaaggcgcta gtctcaaatc aggtatcccc
    53461 tacggttgag ggggattaat gggtatcaca acaagtaaaa aggtataatg atgaaatatg
    53521 actaaaaaat ttattaaatc agcaatgatt ctaggcttag caagcataac catcagcatg
    53581 acgcaacccg ttgaagccat tgcaaatcgc ttagaatatc aagcacaata cgaacgtgtc
    53641 aaagaagaac tagacgcatt aaaagcaaca agtccaaaat taggagaacg ttccctttat
    53701 tttagattgg tgaatagatt tctaggttgg tttggcgtag tctgggaccc actcgcaatc
    53761 aaagaaaccg atcacctgtt ggacctcatc ggacaagtca attacttggt gagtaacacc
    53821 gagtttcaag cccctgaata caaacatgct aaaaacaaac tagacttagc tgtagcaggc
    53881 tatgagctct acctagctag agaaaaagaa cgtggtgata ctcctgatta ccaagtgacc
    53941 cagctaacgt tcaaacagct cgaaaccatt aaaacgaaga tacgagaggc cctggaaaca
    54001 tttgcacaag aggtcaaaac aatccaacaa aaaaacccag acctatttcc ccatgcttat
    54061 gttttaaacg ctaagaccag tgctatcgat ggtaaagcgc aacccttttt agtgaatggt
    54121 tactacgatg tcttttataa gcagttttta ttcactaata aaaatgacgg gtataactat
    54181 aataaagaag cagacaacct aaccttgtat caagatctga ttaaagagct attaggctta
    54241 aaagctgaga tcgatcggtt aacaaaagac aagactgatg atagccgttt agagacgtta
    54301 atgtgggaat caaagttcac cttatctgac tttcggagaa cttcacgcct tagcgcctac
    54361 tatacacaag aagattatgt caaaaaggtg gtcctatctg aagaagctcc caacttgaga
    54421 acagcaaaag aaaaggtcaa gaccttaaaa gggatcttgc atgattacta tgttgacatt
    54481 aaagagccag aaaaagctaa acagtatcat gtcgaggacg aaacctcacc gtctcaacct
    54541 gaggcccctg ctcctggtca aaaaccagca gaagatgagg gatcgtcaca agctacggag
    54601 ccagctaacc cgtcaaaaga ggattcctca acggacgcat ctcagggatc tcacgatagt
    54661 gaaaacccag cgacggataa tccttctcaa cctcaggtac cagaccaagg caatcaccaa
    54721 agccaagtgc ctaacgacaa gcctcaaacg gacaagacgg acactcctaa cgtcccagcc
    54781 tctccacagg acactcctaa cgccccagcc cctccacagg acaccccaaa agtgcttgag
    54841 gctggtggtc agagtggccc cgctgggaac gctgaggaga aagctcctga ctctggacct
    54901 aaagagtctg gtcaaagctc atctaaagag tccccatccg ttggcgaaga cacgacccct
    54961 aatcagccag acgtccttgt aggtggtcaa agtgagccaa acgacatcac cgaagacaca
    55021 atcactgacg ctcctccgac agtatctggg cacaacgcat caacacagcc ccaatctgtg
    55081 gtcgaagaca cagcacctca gcgcccagat gtccttatag gtggtcaaag cgagccaatc
    55141 gatatcaccc aagataccca accgggcatg tcaggctcta atgacgctac cgttatcaat
    55201 gaagatacaa aaccaaaacg cgtcttccac tttggtaaca aagagccaca agcatcagaa
    55261 aaagcgacag aacaacctgc cgttcctcat gatagccaca caacccctca agcttcagat
    55321 gatacagcag cccctcactt gcctgcatct ggagacaagc ctgaaaaagt gtttgcactt
    55381 gctgccttga caatggtcgc agctgcaggg ctactcagca aaaaacgtcg caacaccgac
    55441 aaaaactagt cgctgttagg cacaacgcat cactaaggcc agtgcaaatg cactggcctt
    55501 agttaggttt ttatcaaatt tcatgacaat tgctagcgtt tcagtgtaaa ataaaggtaa
    55561 taaccagcaa aggagcaacc catgacaaag caagatcagt taatcgttga aaaaatggaa
    55621 caaacctatg aggcctttag cccaaagcta gcaaatctca tagaagccct agatgccttt
    55681 aaagagcatt atgaggaata tgccacactt agaaattttt acagtagtga cgaatggttt
    55741 cggcttgcaa accaaccttg ggatgatatc ccgtgcggtg tcctatctga agacctcctc
    55801 tttgatatga tcggtgacca taaccaacta ctagctgata tcttagactt ggcccctatc
    55861 atgtacaagc atatgtaacc attgttaacg tcacagatac tacgatgaca ggacaaaaaa
    55921 agcttgaaaa tctcaaaaaa acagataaaa aacagataat cgttagaaaa acagtagatg
    55981 cgtctgttaa actaaaggta tcaaaacaaa aggagcattt catcatgaaa ttcaaaaaag
    56041 tattagtcat tcctgcatta gcattagcag caacatgctt tttaacagct tgtggcacaa
    56101 aaaaagattc taaaaaagaa gaagttaaag aaatcaaaat gtctgacatc aaagacgacg
    56161 cagttagcaa aaaaacaaaa gtggttgatg gcgaagaagt tacagaatac accacaaaag
    56221 atggaaacgt gattcagatt cctgcaggaa acgaagaagg catggaatca aaagatgctg
    56281 gtggatcagg cgctcctgct aaaaactaag ggttacaaca aagagcataa ggtgactcgc
    56341 aaacgttacg catgacatga ggcagtctgc ctaagatgat aactcactta tcataatgcc
    56401 taaatagcca aacacactaa ctcgcaaatg tacaaaatca tatacccgtg cttatgtgag
    56461 cgaacggtta tatgattttt gttttccaaa aacaagaaaa gaacgctaaa atagccagtt
    56521 tgtcgtgttt caagctacca aaaaggaagt gctaaaatga aaaaaaggtc cattatacct
    56581 gtcatcttac tgttggtcat cggattaatc gttggtgtta agcgtcatca tgactagtag
    56641 gtcttgctag tgaccgttca aaagggataa cgtgggtatg gtataaccat aaaaatcgta
    56701 taaaaagaaa aaatagctat tgaggagaaa gtatgtttca gttaagaaaa aaaatgacgc
    56761 gcaaacaatt agccttgttg agtgctggag tgttgacctg tgtggttggt ggtagctact
    56821 tgataatgaa ccatcaacaa caagaaattg tctccagtgt caacaaagta aaagccttaa
    56881 ccataaaaga agccatggaa caaggaaaag atatcagctt gaccttagct ggtgaagtaa
    56941 cagctaacaa cagcagcaaa gtcaaaatcg actcaagtaa aggagaagtc aaagatgtct
    57001 ttgtcaaaaa aggcgatgtt gtcaaagtag gacaaccctt gtttagctat gaaacgtcac
    57061 agcggttaac ggctcaaagt tcagaatttg atgttcaaac caaagccaat caactccaag
    57121 ttgctaaaac caatgcagca ttgaagtggg aaacctacaa tcgcaaggtc aatgaaatca
    57181 acaccctaaa atctcgctac aacactgcac cagatgagag cttactagag caaattcgca
    57241 gcgcagaaga cagtgtctct caagcactaa gcgatgccaa aacagcagat agcgatgtca
    57301 aaaccgctca aatagaactc gataaagcta atgctactgc cacaacggaa aaaggtaaac
    57361 tagagtatga caccgttaag tcagacaccg caggaaccat tgttagccta aatactgatt
    57421 tgccaaatca atcaaaatcc aaaaaagaaa atgaaacttt tatggaaatt atcgacaaat
    57481 caaaaatgtt agtcaaaggt aacatcagtg aatttgaccg tgacaagtta aaaatcggtc
    57541 aaaaagtcga agtgattgac cgcaaagaca actctaaaaa atggactgga aaagtaaccc
    57601 aagttggcaa cctcaaagca gaggaaaaag gccaaggtca aggccaaggt ggcaatgacc
    57661 aacaagacaa tccaaaccaa gcaaaattcc cttatgttat tgaacttgac caatcagaca
    57721 agcagccact cattggctca cacacttatg ttaatgtgct caacaatgtt ccagaagctg
    57781 gcaagatcgt attgaaagaa acctttacaa tggcagaaaa tggaaaaacc tatgtgtgga
    57841 aagttgataa aaacaaggtc aaaaaacaag aaatcaagac taagccattc tcaaaaggtt
    57901 atgttgaggt gacaagtggc ttgactatgc aagataagat tgctcagccg cttcctggca
    57961 tgaaagacgg tatggaggta ggaagtattg ttaaacctta aagatattcg aaaaagctat
    58021 catcttggga ctgaagaatt tgcgatttta aaaggaatcg atttagaagt taacgagggt
    58081 gactttttag ccatcatggg accatcaggt tcgggaaagt caacattgat gaatatcatt
    58141 gggtgtttag ataagcctgg ctctggctca tatgccattg aaggcagaga cgtgtcatcc
    58201 ttatctgata atgaacttgc tgatttgcgc aatcaaaaaa tcggctttgt ttttcaaaac
    58261 tttaacctga tgcccaagct aacagcttgt caaaatgtcg aattgccctt gacttatatg
    58321 aatgttccta aaaaagagcg tcgcaaacga gccctagaga tgttaaagct agtaggatta
    58381 gaagaacgta gtgaatttaa accgatggag ctatctggtg ggcaaaaaca gcgtgtagcg
    58441 attgcaagag ctttagtcac taacccgagt tttatccttg gtgatgagcc aacaggtgca
    58501 ctagacacaa aaaccagcgt ccaaatcatg gacctattta aacaattcaa tgataacggc
    58561 aaaacgatta ttatcatcac acacgagcct gaagtagctg ccttatgcaa aaagacggtg
    58621 atcctaagag atggtaatat agaacattcc gatatagagt aaagggggaa aataatggaa
    58681 gatattattg cagctttaag ctctatatta tcacacaaga tgcggtcaat tttgacaatg
    58741 ctagggatta ttatcggtat tggtgccatt atcgcaatct tttcaatcat cgaaggtaat
    58801 accgaaaata ccaaaagaca gttgattggt ggcagcaata acactattaa cattgtgttt
    58861 aacaaaaaaa gcagtattga ccctaaattt ccagacaagt caaatgctaa aaaaccagac
    58921 tatcttcctt ttatggctga agaagagctt agcaaaatac agcaagtcaa aggtgtcaaa
    58981 aacgccctga tctcatatgg tattgatgac aaagtctacc atctaggcca aaaatcatct
    59041 gcaaagattt ctgccatcac aaaaaacgta gctgaagtga ggcgcatgac atttattaaa
    59101 ggcagtgact tttcagacaa agactttatt gaccaaaaac aggtgattta ccttgaaaaa
    59161 agcttgtatg agtcactatt tccaaaagac gatggccttg gaaaatttgt cgaagtgatg
    59221 ggaaatccct tcagggtcat tggggtgttt gaatcaaaag aacaaagtgg cctcacaagt
    59281 gggactgaga aaattgctta tatcccgcta catcagtggt ataatattaa tggtgtagtg
    59341 gatgcgacac ctgagatcac gatccagacc tatcgcgcag atgacctcaa gcctgtagca
    59401 aagcgtgtca gtgatatgct taatcaaacg atccctaaat cagattatat gtttggtgtc
    59461 atgaacctta aagaatttga acgccaacta gacaacctaa acaaatctaa ttttgtgtta
    59521 ttagctggta ttgctagtat ctctcttatt gtaggtggta ttggtgtgat gaacattatg
    59581 ctagtatcag ttaccgaaag aacgagagaa attgggatca aaaaagcgct tggcgctaga
    59641 cgtaagctta ttttaaaaca atttttaatt gaagcggtca ttctaacctt gctaggtggt
    59701 gtgatagggg ttatttcagg gatggtatca ggattgatca tcacaagatc actcgagtat
    59761 ccctatatcc tatccttgtt ctcagttgtc ttgagtttag cgttttgttg tattataggg
    59821 attgtctttg gactattacc agccattaaa gcgtctaagc tagacccaat tgaagcattg
    59881 cgatttgaat aatcaaccaa acaagcataa aagtcaaaaa ggatgaccct ttgatgtcat
    59941 ccttagctgc ttgatacact aaacgccaat ctagaaaggg aagttatgtt taaaatatta
    60001 gtagttgaag atgatgatac catcagtcaa gtgatttgtg agtttttaaa agcgaataac
    60061 tatgatccag attgtgtatt tgatggccaa gctgccttag ataagtggca gacaacgtct
    60121 tatgatctga ttattttaga catcatgctc ccttccttaa gtggcttaga agtgctaaaa
    60181 acaattcgaa agacatcaga tgtccctatt attatgctaa cggcattaga tgacgagtac
    60241 acacagcttg taagcttcaa ccatttgatt agtgattatg tgaccaaacc attttcacca
    60301 ctcattttaa tcaaacgtat cgaaaatgta ttacgcgtct ctactccaga tgaaaagcgc
    60361 caaatcggag acttacttgt tgatgagacc gaacatagcg tctattggca aggaacattg
    60421 gtcaaactaa ccaaaaaaga atatgacatt attgattatt tagcaaaacg tcaccaaaag
    60481 attgtcacca gagaccaatt gatggatgac atatggggct attctgaact ggacacgcgt
    60541 gtccttgaca accacatcaa gaaccttcgt aaaaaaatga cgggtattcc cctcaagaca
    60601 attacaggca tgggctattt attgggagag cgtgagtgag actaatcaaa aaaacctttt
    60661 tggtcatcaa tggcttaatt attgtagtgg ttacctctat tttgttagtg ctttattttg
    60721 caatgccaat ttattacacc aaagtcaaag acaaagaagt taagcgtgaa tttgaccaaa
    60781 caagcaagca aatcaaaggc aaaacggtaa ccgagattag agatatccta acaaaaaaaa
    60841 tcaacaaaga taatatttgg tacagcttag tagacagtga taaccaactg ctctatcctt
    60901 ccttgcagct cctagacggc gtcagtgaaa gcaaagacag tcaaaatgtt aatatcgtca
    60961 ccacctttga caactcttat tctaatgtta aggtgatgag tcaaaaagta accctacgcg
    61021 atggaaaaaa aatgacattg cttgggcaat cttcacttca acccgtaaca gatgctagca
    61081 aagttctcct tgacctctac ccgtcactat tgattttttc agtgaccgta gggagtattg
    61141 tcgcctactt atatagtagg acttctagcc ggcgtatcct tagcatgtca caaaccgcca
    61201 aaaaaatggt caacctagaa ccaaacttaa cgtgtaccat tcacggcaaa gatgaaatag
    61261 caatgttggc tagtgatatt aaccgtttgt atgcgagtct ttcaacgagc atcaaatcct
    61321 tgcaaaaaga gtatgaaaaa gcttctgatt cagaaagaga gaaatctgag tttttacgca
    61381 tgacatcgca cgagctaaag acaccaatca ctagtgttat cggaatgatt gatggaatgc
    61441 tctataatgt tggtgacttt gcagatcgtg acaaatattt gcgaaaatgc cgagacgtcc
    61501 ttgaagggca agcccaactg gttcaatcca ttttatcttt atctaagatt gaaaccctag
    61561 cttctcaaaa tcaagaactg ttttcactaa aaagcagcct agaagaagaa atggaagtct
    61621 ttctcgtttt atcagaacta aaacacctca aagtgactat caatctcgaa gaacaattcg
    61681 tcaaagccaa taaagtatac ctactaaagg cgattaaaaa tattattgac aatgcctttc
    61741 actataccaa atcaggcggc caagtgatga ttcaactaaa agacaaccaa ctagtgatta
    61801 aaaatgaagc agagacatta ttgacacaac agcagatgaa acagttattc caaccgtttt
    61861 atcgaccaga ttatagtcgt aacaggaaag acggtggtac aggattgggg ctgtttatta
    61921 cccaccagat tcttgatcag catcatctgg cttatcgctt tgttgttctt gatcaaagat
    61981 ggatggtatt tacgattgat tttccatccc atcatgacga ttgatccaca ctagtaggac
    62041 gacaaaagtc atcaatcatt aaccaagcct tgacctttac ccatcattgt ttaggcaagt
    62101 ataaaaaaga ggagagaaat catatatgaa gaaaaggaaa ttgttagcag taacactatt
    62161 aagtaccata ctcttaaaca gtgcagtgcc attagttgtt gctgacacct ccttgcgtaa
    62221 taacccatca tccactgatc agcctactac agcagatact gatacggatg acgagagtga
    62281 aacagcaaaa aaagacaaaa aaagcaagga aacagcgtcg cagcacgaca cccaaaaaga
    62341 ccataagcca tcacacaatc acccaacccc cccttcaaat gatactaagc agaccgatca
    62401 ggcatcatct gaagctactg acaaaccaaa taaagacaaa aacgacacca agcaaccaga
    62461 cagcagtgat caatccaccc catctcccaa agaccagtcg tctcaaaaag agtcacaaaa
    62521 caaagatggc cgacctaccc catcacctga tcagcaaaaa gatcagacac ctgataaaac
    62581 accagaaaaa tcagctgata aaacccctga aaaaggacca gaaaaagcaa ctgaaaaaac
    62641 tccagagcca aatcgtgacg ctccaaaacc catccaacct cctttagcag cagcagctcc
    62701 tgtctttgga ctttggagag aaagtgacaa agacctgagc aagctaaaac caagcagccg
    62761 ctcatcagcc gcttacgtga gacactggac aggtgactct gcctacactc acaacctgtt
    62821 gtcacgccgt tatgggatta ctgctgaaca gctagatggt tttttgaaca gtctaggtat
    62881 tcactatgat aaagaacgct taaacggaaa gcgtttatta gaatgggaaa aactaacagg
    62941 actagacgtt cgagctatcg tagctattgc aatggcagaa agctcactag gtactcaggg
    63001 agttgctaaa gaaaaagggg ccaatatgtt tggttatggt gcccttgact tcaacccaaa
    63061 caatgccaaa aaatacagcg atgaggttgc tattcgtcac atggtagaag acaccatcat
    63121 tgccaacaaa aaccaaacct ttgaaagaca agacctcaaa gcaaaaaaat ggtcactagg
    63181 ccagttggat accttgattg atggtggggt ttactttaca gatacaagtg gcagtgggca
    63241 aagacgagca gatatcatga ccaaactaga ccaatggata gatgatcatg gaagcacacc
    63301 tgagattcca gaacatctca agataacttc cgggacacaa tttagcgaag tgcccgtagg
    63361 ttataaaaga agtcagccac aaaacgtttt gacctacaag tcagagacct acagctttgg
    63421 ccaatgcact tggtacgcct ataatcgtgt caaagagcta ggttatcaag tcgacaggta
    63481 catgggtaac ggtggcgact ggcagcgcaa gccaggcttt gtgaccaccc ataaacctaa
    63541 agtgggctat gtcgtctcat ttgcaccagg ccaagcagga gcagatgcaa cctatggtca
    63601 cgtcgctgtc gtagagcaaa tcaaagaaga tggttccatc ttgatttcag agtcaaatgt
    63661 tatgggatta ggcaccattt cctatcggac gttcacagct gagcaggcta gtttgttgac
    63721 ctatgtcgta ggggacaaac tcccaagacc ataaccccac aaccaagaag ctacctgcct
    63781 gttgaccaat ctcaaaaaga gatagcgtca acttagaatg aggttcatca gatgataaag
    63841 aaagtgacca ccccgagtca aaaaacaaaa aaacgtgttc gtaatggata cctcctaaaa
    63901 gtaggtacag cctgtttact cttaagtata ctcagttatg gcattggtct tttgggccag
    63961 cctagcatgg aaaatacgtt tatgggaata gcgagtgttg ccatgttagg aagtgtctgt
    64021 ttctttatta tctttgctct aaaccgtatc tttgacgcac tagaggacaa tttgcgagat
    64081 tagagtaata ggtcaaataa tcttccaata atttagagca caagctaagt tattggaagt
    64141 ttttttgtct tttgagagcc atgatgccat caaaaagcca aacactcctt atgatgtgag
    64201 agtcacatga ctaaaattaa aagcaaaata gaccccaaag ccctgttttc tcaaaacagt
    64261 caacgaaaaa agacaagtta accagttcat aaaaaacatc gtagaaacat tttcaagagc
    64321 taatgttggt gtaaattgac tgaagtatga tagaattttt aatgaatgtg acaat
//
LOCUS       DAUCWO010000004        58319 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUCWO010000004 DAUCWO010000000
VERSION     DAUCWO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58319
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.004"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            73..606
                     /locus_tag="VGY20_000214"
     CDS             73..606
                     /locus_tag="VGY20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ4273102.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            873..1559
                     /gene="covR"
                     /locus_tag="VGY20_000215"
                     /gene_synonym="csrR"
     CDS             873..1559
                     /gene="covR"
                     /locus_tag="VGY20_000215"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ4273103.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            1565..3067
                     /gene="covS"
                     /locus_tag="VGY20_000216"
                     /gene_synonym="csrS"
     CDS             1565..3067
                     /gene="covS"
                     /locus_tag="VGY20_000216"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ4273104.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQTVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            3282..3776
                     /gene="nrdR"
                     /locus_tag="VGY20_000217"
     CDS             3282..3776
                     /gene="nrdR"
                     /locus_tag="VGY20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ4273105.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            3760..4935
                     /locus_tag="VGY20_000218"
     CDS             3760..4935
                     /locus_tag="VGY20_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ4273106.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQENLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSCRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            4936..5838
                     /gene="dnaI"
                     /locus_tag="VGY20_000219"
     CDS             4936..5838
                     /gene="dnaI"
                     /locus_tag="VGY20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ4273107.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            5901..7211
                     /gene="der"
                     /locus_tag="VGY20_000220"
     CDS             5901..7211
                     /gene="der"
                     /locus_tag="VGY20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ4273108.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            7418..10516
                     /locus_tag="VGY20_000221"
     CDS             7418..10516
                     /locus_tag="VGY20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4273109.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            10759..11361
                     /locus_tag="VGY20_000222"
     CDS             10759..11361
                     /locus_tag="VGY20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273110.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            11401..12729
                     /locus_tag="VGY20_000223"
     CDS             11401..12729
                     /locus_tag="VGY20_000223"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ4273111.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            12775..13257
                     /locus_tag="VGY20_000224"
     CDS             12775..13257
                     /locus_tag="VGY20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4273112.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            13369..14943
                     /gene="mltG"
                     /locus_tag="VGY20_000225"
     CDS             13369..14943
                     /gene="mltG"
                     /locus_tag="VGY20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ4273113.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAAKRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            15016..15498
                     /gene="greA"
                     /locus_tag="VGY20_000226"
     CDS             15016..15498
                     /gene="greA"
                     /locus_tag="VGY20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ4273114.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(16118..17041)
                     /gene="yidC"
                     /locus_tag="VGY20_000227"
     CDS             complement(16118..17041)
                     /gene="yidC"
                     /locus_tag="VGY20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ4273115.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITAYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(17123..17401)
                     /locus_tag="VGY20_000228"
     CDS             complement(17123..17401)
                     /locus_tag="VGY20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ4273116.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            17662..18399
                     /locus_tag="VGY20_000229"
     CDS             17662..18399
                     /locus_tag="VGY20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ4273117.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            18438..18938
                     /locus_tag="VGY20_000230"
     CDS             18438..18938
                     /locus_tag="VGY20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ4273118.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            18953..19642
                     /locus_tag="VGY20_000231"
     CDS             18953..19642
                     /locus_tag="VGY20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ4273119.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            19820..20062
                     /locus_tag="VGY20_000232"
     CDS             19820..20062
                     /locus_tag="VGY20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ4273120.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            20240..21034
                     /gene="racE"
                     /locus_tag="VGY20_000233"
     CDS             20240..21034
                     /gene="racE"
                     /locus_tag="VGY20_000233"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ4273121.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSIQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLSPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            21031..22017
                     /locus_tag="VGY20_000234"
     CDS             21031..22017
                     /locus_tag="VGY20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ4273122.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKVKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQRFGDTVLIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNTKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            21996..22517
                     /locus_tag="VGY20_000235"
     CDS             21996..22517
                     /locus_tag="VGY20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ4273123.1"
                     /translation="MASETIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            22514..22975
                     /locus_tag="VGY20_000236"
     CDS             22514..22975
                     /locus_tag="VGY20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ4273124.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            22972..23718
                     /gene="xerD"
                     /locus_tag="VGY20_000237"
     CDS             22972..23718
                     /gene="xerD"
                     /locus_tag="VGY20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ4273125.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            23718..24422
                     /locus_tag="VGY20_000238"
     CDS             23718..24422
                     /locus_tag="VGY20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ4273126.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IEPDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            24419..24970
                     /locus_tag="VGY20_000239"
     CDS             24419..24970
                     /locus_tag="VGY20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ4273127.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            25091..25813
                     /locus_tag="VGY20_000240"
     CDS             25091..25813
                     /locus_tag="VGY20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4273128.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLGLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            25810..26073
                     /gene="yidD"
                     /locus_tag="VGY20_000241"
     CDS             25810..26073
                     /gene="yidD"
                     /locus_tag="VGY20_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ4273129.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            26251..26799
                     /locus_tag="VGY20_000242"
     CDS             26251..26799
                     /locus_tag="VGY20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEQ4273130.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNVARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      26908..27031
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27110..27673
                     /locus_tag="VGY20_000243"
     CDS             27110..27673
                     /locus_tag="VGY20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4273131.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            27675..28328
                     /locus_tag="VGY20_000244"
     CDS             27675..28328
                     /locus_tag="VGY20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ4273132.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQD"
     gene            28621..29541
                     /locus_tag="VGY20_000245"
     CDS             28621..29541
                     /locus_tag="VGY20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ4273133.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            29580..30161
                     /locus_tag="VGY20_000246"
     CDS             29580..30161
                     /locus_tag="VGY20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4273134.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVSAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADVYPLSLCQVY"
     gene            30267..31601
                     /locus_tag="VGY20_000247"
     CDS             30267..31601
                     /locus_tag="VGY20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ4273135.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            31679..32470
                     /gene="pflA"
                     /locus_tag="VGY20_000248"
     CDS             31679..32470
                     /gene="pflA"
                     /locus_tag="VGY20_000248"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ4273136.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQINFITALFIEA
                     KKLGIHTTLDTCGFAYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            32601..33536
                     /locus_tag="VGY20_000249"
     CDS             32601..33536
                     /locus_tag="VGY20_000249"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ4273137.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            33612..34265
                     /locus_tag="VGY20_000250"
     CDS             33612..34265
                     /locus_tag="VGY20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ4273138.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDYVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVVIKEKTNITRDDLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(34310..35347)
                     /locus_tag="VGY20_000251"
     CDS             complement(34310..35347)
                     /locus_tag="VGY20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4273139.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLVHVFLGKSSHAISFIVINIRLPRILAAFLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(35308..36360)
                     /locus_tag="VGY20_000252"
     CDS             complement(35308..36360)
                     /locus_tag="VGY20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4273140.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(36350..37282)
                     /locus_tag="VGY20_000253"
     CDS             complement(36350..37282)
                     /locus_tag="VGY20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ4273141.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIVDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(37308..38090)
                     /locus_tag="VGY20_000254"
     CDS             complement(37308..38090)
                     /locus_tag="VGY20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273142.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(38336..39781)
                     /locus_tag="VGY20_000255"
     CDS             complement(38336..39781)
                     /locus_tag="VGY20_000255"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ4273143.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            39869..41503
                     /locus_tag="VGY20_000256"
     CDS             39869..41503
                     /locus_tag="VGY20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ4273144.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSVIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFFIMKLGSKDYLSAVTQSTLAAFVGMIASMAVLGY
                     YLWKLGLLKAICRKPNHHIDSDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLLALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            41670..42299
                     /gene="upp"
                     /locus_tag="VGY20_000257"
     CDS             41670..42299
                     /gene="upp"
                     /locus_tag="VGY20_000257"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ4273145.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            42523..43113
                     /locus_tag="VGY20_000258"
     CDS             42523..43113
                     /locus_tag="VGY20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ4273146.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            43608..43901
                     /locus_tag="VGY20_000259"
     CDS             43608..43901
                     /locus_tag="VGY20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ4273147.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKGYQPPSY"
     gene            44132..44767
                     /locus_tag="VGY20_000260"
     CDS             44132..44767
                     /locus_tag="VGY20_000260"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ4273148.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVTQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            44785..45660
                     /locus_tag="VGY20_000261"
     CDS             44785..45660
                     /locus_tag="VGY20_000261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ4273149.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     GKRQVFIIKDCEKMHVNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLFQLAQERGLLRSQADLVAKLAKNVDQLEDLAQNSHFLEVMAQTERFVSIWL
                     KDQLQAYLALNHLVLLATEKEEQELILTLLTLLLAREQSQTPFKQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            45679..45918
                     /locus_tag="VGY20_000262"
     CDS             45679..45918
                     /locus_tag="VGY20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
                     /protein_id="HEQ4273150.1"
                     /translation="MGKRSLSKDLPVATKVAAHRGKKAFEENTALATDLMKEVSRLMI
                     QNQLEMTFINSVFPLDQHVVLIPFSAEEHMDFYNC"
     gene            46323..46646
                     /gene="yabA"
                     /locus_tag="VGY20_000263"
     CDS             46323..46646
                     /gene="yabA"
                     /locus_tag="VGY20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ4273151.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            46651..47514
                     /gene="rsmI"
                     /locus_tag="VGY20_000264"
     CDS             46651..47514
                     /gene="rsmI"
                     /locus_tag="VGY20_000264"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ4273152.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYTETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEIELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            47541..47933
                     /locus_tag="VGY20_000265"
     CDS             47541..47933
                     /locus_tag="VGY20_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273153.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(47980..48609)
                     /locus_tag="VGY20_000266"
     CDS             complement(47980..48609)
                     /locus_tag="VGY20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ4273154.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENH
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQNLSQLTGTAIVHGTKII"
     gene            48908..49264
                     /locus_tag="VGY20_000267"
     CDS             48908..49264
                     /locus_tag="VGY20_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ4273155.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEVIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGR
                     VLQVGYRKPYQELDLG"
     gene            complement(49338..50165)
                     /gene="xth"
                     /locus_tag="VGY20_000268"
     CDS             complement(49338..50165)
                     /gene="xth"
                     /locus_tag="VGY20_000268"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ4273156.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIEALLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            50399..51580
                     /gene="lctO"
                     /locus_tag="VGY20_000269"
     CDS             50399..51580
                     /gene="lctO"
                     /locus_tag="VGY20_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ4273157.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSTGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            51844..56787
                     /gene="scpC"
                     /locus_tag="VGY20_000270"
     CDS             51844..56787
                     /gene="scpC"
                     /locus_tag="VGY20_000270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ4273158.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESKPQDTSQITPKTNREKEQSQDLVSEPTTTELADTDS
                     APMANTGSDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKNIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTSNGMGIPSAFISHEFGKAMSQLNTNGTGSLVFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVTVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLPQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKMYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTAFATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            57558..58265
                     /locus_tag="VGY20_000271"
     CDS             57558..58265
                     /locus_tag="VGY20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ4273159.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVML
                     TGLAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
BASE COUNT        18717 a        10483 c        11989 g        17130 t
ORIGIN      
        1 ataaatgcaa aactattgac aaatctttta gatagtagta aaaataaatg agagttttat
       61 taggagaaga tgatgttagc tatttccgaa atcagaaaac acccagacgg tctctcgttt
      121 gatcgtttat gtgatgtcaa aagtatgtta cttgagcgtg accaacagat tattgacata
      181 aaggcagtta aagctgtcgg taatgttcgc tatgataagg ggctttatct gttagattat
      241 caattgagtt acgaagtgat attaccgtct agccgttcta tggttccggt ctgtttaagt
      301 gaagtccaac acattcaaga attatttata gaggcgacag atttagctga taagaaagag
      361 ttggttgaag acaatctggt attagtttta gacaaagacg ctattaacct cgaagaaagt
      421 attgtggata atatcttact tgctattccg ctacaggtct tgacagagga agaaaagaaa
      481 tcaaaagagt tgccagcagg tcaaaattgg gcagttttga cagaagaaga ctaccagtgt
      541 ctaaaagaag aaaagcaaaa agaaaataat ccttttgcta gcttgcaagg gttgtttgat
      601 gaataaaact gaatattaaa gatgtctgta atttttattt gaaaaaacag ctattctttg
      661 atatactctt tagagaatat ggttacttat ctaaagtaga aggtattttg accatagagg
      721 gcaaagaagg taatgactat tagatgcttc atattttatt cgctagaaga ctatttgacc
      781 attagaactg aaatagtcta ggatatgaga tggatttcat agttttcttt ctgggagaaa
      841 aagatagatt aagaggataa gggttggtat aaatgacaaa gaagatttta attattgaag
      901 atgaaaagaa tctggctaga ttcgtttctc ttgagctgca acatgagggt tatgaagtca
      961 ttgttgaggt caatggtcgt gaagggttag aaactgcttt ggaaaaagag tttgatttaa
     1021 tcctgcttga cttaatgtta ccagagatgg atggttttga agtgacccgt cgtttgcaaa
     1081 ccgaaaaaac aacgtatatc atgatgatga ctgcgcgtga ttctattatg gatgtggttg
     1141 caggtttaga ccgcggtgca gacgactata ttgttaaacc gtttgccatt gaagaactac
     1201 ttgcccgtat tcgtgctatt ttccgccgtc aagatattga atctgagaag aaagtgccta
     1261 gtcaaggcat ttatcgagat ctagttttaa atccacaaaa ccgttcagtt aatcgtggcg
     1321 acgatgagat ttctctcact aaacgtgaat atgatttgct taatattttg atgactaata
     1381 tgaatcgtgt catgacacgt gaagaattat tgtcaaatgt ttggaaatat gatgaagccg
     1441 ttgagactaa tgttgtagat gtctatattc gttatctccg cggcaaaatt gacattccag
     1501 gcaaggaatc ttatatccaa acagtgcgtg gcatgggata cgttattcgt gagaaataag
     1561 tcatatggaa aatcagaaac aaaaacagaa gaaatataaa aactcgttac caaaacgact
     1621 atctaatatc ttttttgttc tttttttctg cattttctct gcctttacac tgattgccta
     1681 tagttcaaca aactatttct tattgaagaa agaaaagcag tcagtctttc aagctgtaaa
     1741 tattgttaga gttcgtcttt ctgaggtgga ctctaatttt acattagaga acttagcaga
     1801 agttttgtac aaaaacgata aaacacatct gagaattgat gacagaaagg gcagtcgagt
     1861 cattaggagt gagcgtgata tcacaaatac tctagatgcg aatcaagata tttatgtcta
     1921 taacattgat aaacagatga tttttaccac agataacgaa gaatcatctc ctggcttgca
     1981 tggtcctatc ggtcgtgtgt atcacgacca tatcgaagat cagtatcgtg ggttttccat
     2041 gacacaaaag gtatattcta atcggactgg aaaatttgtg ggctatgttc aagtctttca
     2101 tgatttaggc aattattatg tcattagagc aagactgctg ttttggctac tagttgttga
     2161 gttatttggc acaagcttag cctatttaat catcttaatt actacgcggc gctttttgaa
     2221 gcctttacac aatttacatg aagtgatgcg taatatctct gaaaatccta ataatttaaa
     2281 cctgcgctca gatatttcgt caggagatga aatcgaagaa ttgtctgtta tctttgacaa
     2341 tatgttagac aaattggaga cacatactaa gttgcaatca cgttttatta gtgatgtcag
     2401 ccatgaatta cgaacaccgg ttgcgattat taaagggcat attggtctct tacaacgttg
     2461 gggtaaagat gacagtgata ttcttgaaga aagcttgaca gcaacagcgc atgaggctga
     2521 ccgtatggca atcatgatta atgatatgtt agacatgatt cgcgtacaag gctcatttga
     2581 gggacatcaa aatgatatga cagttttgga agattctatt gaaactgttg ttggtaattt
     2641 tagagtttta agagaagatt ttatctttac atggcagtca gaaaatccaa aaacgatagc
     2701 ccgtatttat aaaaatcatt ttgagcaggc tttgatgatt ctgattgaca atgctgtgaa
     2761 gtattcccgt aaagaaaaga aaatcgcgat taacctttca gtgactggca aacaagaagc
     2821 tattgttaga gttcaagata aaggcgaagg aatttctaaa gaagatattg aacatatctt
     2881 tgaacgcttt tatagaacgg ataaatcacg taatcgaaca agtacccagg ctggattagg
     2941 gattggcttg tctattctca agcaaactgt agatgggtat catttacaga tgaaggttga
     3001 aagtgaatta aatgaaggtt cagtgtttat cttacatatt cctttggccc agtctaaaga
     3061 gagttagagt agcttaaggc attgttaatg tgacaggtat agtatgtaat cattagataa
     3121 tagtagcagt cttttgccac aactggttag aagctgatgc tattttttta gcaatgatgg
     3181 atgacatgaa agaaatactg tcaatcgcga tgccttttgt tatccttgag aatatggtaa
     3241 aatagtgtat tagattaatt tgatagaatt ggggtgtttg cgtgcgttgt ccaaaatgta
     3301 attaccataa atcgagtgtt gttgacagta gacaagctga agacggcaac accattcgtc
     3361 gtcgaagaga atgtgaacag tgtcatactc gctttactac ctttgagcgt gttgaagaac
     3421 tccctttatt agtcatcaaa aaagatggca cacgagagca attttctaga gataaaatat
     3481 taaatggggt tgtccaaagt gcgcaaaaac gacctgtctc gagcacagat attgaaaatg
     3541 tcatttctcg tattgaacaa gaagttcgaa caacctacga aaatgaagtg tcaagtactg
     3601 ctattggtaa tttagtgatg gatgagttgg ccgaactcga tgaaatcaca tatgtacgct
     3661 ttgctagtgt ctacaagagc ttcaaggacg ttgatgaaat cgaagaactt ttgcaacaaa
     3721 ttacgaatcg cgtacgcgga aagaaaaaac gtttaaataa tgatgaaacc aattgatact
     3781 tttacatatc taaaacgcaa caaagtaact cttgattcgg tgactttaat acagttgtac
     3841 tttccaatta ttggaagtga cgcagtctct atctatcaat actttattca cttttttgac
     3901 gatggtttac aacgtcataa attcagcgat attttaaatc acctacagtt tggtatgaaa
     3961 cgttttgaag atgctcttgc catattgaca gcaatggaat tagtgagtgt gtatcagcta
     4021 tcagatacat atctcattac gttgcaccag ccgcttagcc gtgacttgtt ttttcaacat
     4081 ccagcttatt ctcgattatt agaacaaaag attggagagg tagctgtctc tgagttgcag
     4141 gtaactgtcc caagtcaagc aagaaatatt tctaagcgat tttcggatat ttttggggtg
     4201 caagaaaatt tgacgaacgt acctcaaaag ccacaaaaga attttgacct ttcaagtttc
     4261 cagcaattaa tggttcgtga tgggttacaa tttgaagata atcaaaaaga tatcattagc
     4321 ctttacagta ttgctgaaca gtatgatatg acgtggtttg atacttatca aatagctaag
     4381 gcgacggctg ttaatggaaa aattcgacct gaacgcttat tggctaaaaa aaatcaaagc
     4441 atgacaaaac caagcaaaga aaatttttca caggctgagc aaattatttt gcgagaagca
     4501 aagcaagaca gtgctttagt ctttttggaa aaaattaaaa aggctagacg tgcaacgatt
     4561 actaaggatg aacgcatttt attgcaaaca ttggccaaaa tgaattttct tgatgatgtt
     4621 atcaatgtca tggtgcttta tacctttaat aagacaaaat ctgctaattt acagaagagt
     4681 tatgtcttaa aaatggccaa tgattttgcc taccaaaagg tgtcaacggc tgaagaagct
     4741 atagtggttt tgcgagcctt cacggatcgt caaagttgtc ggcaatcaaa agtaaaaaca
     4801 agtcaaagca atgtgcctaa gtggagcaat cctgattacc aagagacaac aagtcaagaa
     4861 gagcaggcga aactagatca gttcaaacag gctgctttga agaggttaga aaacctagga
     4921 aaagggggtg actagatgga aaagattgga gaaaccatgg cgaaattggg tcaaaacact
     4981 cgtgttaata gcgaccagtt gattcaaacc attttagcgg atcctgaagt agcaagcttc
     5041 atcagtcaac atcacttatc tcaagagcaa attaacctta gcttatctaa gttcaatcaa
     5101 tttttggtgg aacgccagaa gtatcaactc aaagatccct cttatattgc taagggctat
     5161 cagcctattc tagctatgaa tgagggctac gctgatgtgt cttatttgga aaccaaagaa
     5221 ttggtagaag ctcaaaaaca ggcagctatc tctgaacgga ttcaactggt tagtttgcca
     5281 aaatcctatc gccatattca tttatcagat attgatgtta ataatgcgag ccgtatggaa
     5341 gccttctctg ctattttaga ttttgtggaa caatacccat cagcagagca aaaaggctta
     5401 tatttgtacg gagatatggg aattggcaag tcttacttgt tggctgctat ggcccatgaa
     5461 ttgtctgaga aaaaaggtgt gtcaacgacc cttctacatt ttccgagctt tgccattgat
     5521 gtaaaaaatg ccatttcaaa cggctctgtt aaagaagaaa tcgatgcggt caaaaatgta
     5581 ccagtgctca ttttagatga tattggtgca gagcaagcca cttcttgggt ccgtgatgag
     5641 gtgttgcagg tgattttaca ataccgtatg ttagaggaat tgccaacctt ctttacctct
     5701 aattacagct ttgcggattt ggagcgaaaa tgggcaacca tcaaaggaag tgacgaaact
     5761 tggcaggcca aacgtgtcat ggaacgcgtg cgttatttgg ctagagagtt tcatttagaa
     5821 ggggctaacc gccgttaagt ttgagatagc aatcaagaag aatagctaca cgtatgagtg
     5881 agtgaaagaa aaggagaatc atggttttac ctaccgttgc cattgtgggc cgtccaaacg
     5941 tgggcaagtc caccttattt aatcgaatcg caggagagcg catttcaatt gtagaagacg
     6001 ttgaaggggt tactcgtgac cgtatttatg ctactgggga gtggcttaac cgccaatttt
     6061 cattgattga tacaggtggt attgatgacg ttgatgcccc gtttatggag caaatcaagc
     6121 accaggctca aattgccatg gaagaagctg atgttatcgt ttttgttgtt tctggcaaag
     6181 aaggggttac ggatgcagat gaatatgtgt ctaaaattct gtatcgaacc aataccccag
     6241 tcattttagc tgttaataag gttgataatc ctgaaatgcg taatgacatt tatgattttt
     6301 attcacttgg tttaggggat ccctatccag tttcttctgt tcatggtatc ggaacagggg
     6361 atgtcttgga tgcgatcgtt gaaaacttgc cagtagaaga ggctgaagaa aatgatgata
     6421 ttattcgctt cagtttaatc ggtcgtccta atgttgggaa atctagcttg attaacgcta
     6481 ttttaggtga ggaccgtgtt atcgctagcc ctgtcgcagg gacaacacgt gacgctattg
     6541 acacgcattt tacggatgct gacggtcaag aatttaccat gattgacacg gctggaatgc
     6601 gcaaatctgg caagatttac gaaaatacag agaaatattc tgtcatgcgt gccatgcgtg
     6661 ccattgaccg ctccgatgtg gtcttgatgg ttatcaatgc tgaagaaggt atccgtgaat
     6721 acgacaagcg tatcgctggc tttgcacacg aagctggtaa aggaatgatt atcgtggtta
     6781 acaaatggga taccattgac aaggataatc ataccgttgc taagtgggaa gccgatattc
     6841 gtgaccaatt ccaatttttg acatacgctc cgattatttt tgtgtcagct ttgacgaagc
     6901 agcgcctcaa caaattgcct gacttgatta aacgtatcag tgagagtcag aataaacgta
     6961 tcccatctgc tgtcctcaat gatgttatca tggatgctat tgccattaat ccaacgccaa
     7021 cggataaggg taaacgtttg aaaattttct atgccactca agtgtcagta aagccaccaa
     7081 catttgtcgt ctttgttaac gaagaagaat tgatgcactt ctcttatcta cgcttcttgg
     7141 aaaaccagat tcgcgcagcc tttacctttg aagggacacc gattcatctg attgctcgca
     7201 aacggaaata agaaatgaac atcaactaaa atgctgacct aagaaataat ctcatcattg
     7261 ataaggactg tgttgtgtat gttacaggag acagtccttt ttataatacc tttaagggtg
     7321 tagacggaag attagtagca aacaaagaaa aaggattttg acaagaaata tcagtcgctt
     7381 tttgctataa tgaaaggata agcaaaaagg tggaaaaatg atggctagat taattccagg
     7441 acgagtaagg aatgaaggta ttaagcttta tgagcaaggt ttagtctcat tccaagatga
     7501 taataaaggc attcttcaaa tagaagtgga gacttatcag gttcaatatg gagcagatga
     7561 tgaggatatc acttgtcaat gcgacacatt tcatatgaaa cattattgca agcatattgc
     7621 agcagtcgag tattttttaa aaaatgatca aaaaggtaag ctttttttaa aacagttaac
     7681 aaatcaaaca aaaatcaaag aaacaacaaa gaaaatgaca tcttttggta gtctcttttt
     7741 agatagctta gccatgaatg aagatgattc tgttaaatac aggctgtctg ctcttggtag
     7801 tcgaagccct ttttcgtcag attactggtg gagtctgaaa atcaatcgtt taccagatga
     7861 tcgttcttat gtgattcgag atattaaagg ctttttgcag cttattaaaa aagaaggctt
     7921 ttatcagatt ggaaaaaatt attttgaaca actttcatgg ctgcagtttg acccatccag
     7981 tcaagacctt attgagtttt tatggcgctt agcatctaat actgacaaag gggacaacga
     8041 gaacattttt ccaaatcatg caaggcattt gcgcttacct agtggttttt ttgaagaagg
     8101 tattcactat ttgacgtcac tttatgattt tacttttgag gggccttctc aaacttatca
     8161 tcatcttttt gtccgttctt tggaagcaga ggcaggcctt tatgagttta aagtggaagt
     8221 gcatcgcaaa tctattgaat tgcaaattgc tgaaaaaaat gtgcaatacc tctttgataa
     8281 tgactatctt ctttatcaag atacctttta tcacttgacg ttaaagcaac gaaaaatggt
     8341 tcaggctatt cgtagtctgc ccattgaggc cgacttggct aaacatattc attttgattt
     8401 ggatgatcat gcgaaattag ctgcaagctt atcagatttt aaacagatcg gtctagtaga
     8461 ggctcctaaa agctttgcta ttagagactt tgaggtgact tttcaatttg atttgttaaa
     8521 tcgagatgaa atcagttgtc agcttatgtt tgattatggc aattaccaag ttagtgacaa
     8581 agcaagttta gaagcacttc cttttgctag tcatttaaaa aaagaagaaa aaatcaatcg
     8641 ttctttactt gcttttggct tctcgccaca attttactca aaaaagcgac taacaagtgc
     8701 aaaagagctc tatacttttt ttgaagaaac agttccatgt tttgagcgct taggtaatgt
     8761 tgcgttgagt acagctatcc aagcattaca agtgaaagaa atgcccaaga tcgctattag
     8821 acgcaatcag ggattactag atatttcgtt tgatttttca accattattg aaaatgatat
     8881 tgaccaagct gttacagctc tttttcaaaa taatccttat tttgtgagtc aaacaggtca
     8941 attagtggtt tttgatgatg aaactcaaaa agttagcaaa agtttacaag aattaagagc
     9001 aagacagcta aaaaatggtc acttgcaact ggatggcatt agagctctac aagtatccaa
     9061 actttttgaa ggcatgacat ctgttcattt ttcaaaggaa ttagaagagt tagcctatca
     9121 tttgcagcat cctgaaactt tttcaataaa accgttacca gttaaggctc aaatgcgaga
     9181 ttatcaacgc aacggtgttc agtggttatc aatgctcaat cattatggct ttggtggcat
     9241 tttagcagat gacatgggac ttggaaaaac tctccagaca cttgcttttt tagctagtca
     9301 cctgaaatca gatagtaaag tgctcatttt atctccatct agtcttatct ataattggtt
     9361 tgatgaatgt caaaaattca cacctcagct agatgttgta gtttcttacg gacttaaaca
     9421 aatcagagat caaatcatcg aagaaggtca tcaaataacc ataaccagct attcctcctt
     9481 tagacaggat tttgagacct atcaagcgtt tcactacgat tatcttatct tagatgaagc
     9541 tcaagtgata aaaaatgcgc aaactaagat ttcacactgt ttacgtgcgt ttaatacagc
     9601 caattgtttt gcgctttcgg gcactcctat tgaaaataaa atgcttgaaa tttggtccat
     9661 tttccaaatt gttttgccag ggttgctacc gactaaaaaa gagtttttaa aattaacagc
     9721 tgagcaagta tcgcgctata tcaaaccctt tgtgatgcgt cgtaaaaaag aagatgtcct
     9781 accagaatta cctgatttaa ttgaaactaa ttattcaaac gagatgacag atgaacaaaa
     9841 agctatttac ttagcgcagt taaggcagat gaaagatcaa attcgaaatt ctagtgatgt
     9901 ggatattagc cgtcaaaaaa ttgagatttt atctggtatc actcgattga ggcaaatctg
     9961 tgacactcca agtctcttta tggattacca aggcaaaagt ggtaagctag atagtttgcg
    10021 aatcttattg acacaaatta aagaaaatgg tcatcgggct ttgatttttt ctcaatttag
    10081 agggatgctg gacttggcca agcaagagat gactgctcta ggattgacct cttatcaaat
    10141 gacgggatct acccctgcca atgaacgtca agaaatgact agagccttta ataacggttc
    10201 caaggatgct tttttgattt ccctcaaggc aggtggagtt ggaatcaacc ttacgggagc
    10261 agacacagtg attttaatcg atttatggtg gaatcctgct gttgaaatgc aggcaatcag
    10321 tcgcgcctac cgtattggtc aaaaagaaaa cgttgaagta tatcgcttaa tcactcgagg
    10381 gacgattgaa gaaaagattt tagagcttca agaaagtaag cgcaatttgg tgactacagt
    10441 tttggatggc aatgaaagcc gagcaagtat gtccattgaa gaaataaaag aaattttggg
    10501 gctaaacact cattaatctt ccttgatttt agtgacatta aaaacccttt aaagaagatg
    10561 acaccaaaag caccttatat aacctcgtta aaacgcttct ttttgaaata tctttattga
    10621 tatagcgatt gtcacttgct agtaatgatt aaaacattta gaaaaagaca agtcatagaa
    10681 tcatcaagga atgctttgaa aaataacagt atttaagtta taatagagga tgctacaaga
    10741 agggagaatc aaaaaaggat ggtaaaaaat cagtttcctt tagttgcaga tggcattgct
    10801 attagtgatc cagcaaagca aatggctctg tatgaaaatg aagatctcat taccaacatc
    10861 cgtggctatt accaggacaa ggagtatgat gatattgcta gaaatgagga gtttactgct
    10921 aaagcaactt ctcggcaggc cccttctagt aagcgctttt gtagcaatga tgaaaaacac
    10981 cattatgtca aagaggcgcg tcaaaaagca aagcaagatc tcaaggaaaa aagacaggct
    11041 tatcttgcca aagaaatggc ctatgttcct aaacaggttt ctaaaaagca acagcctgcg
    11101 gattcttctc cttctcaaaa gcaagcaaca acagaaatga gtcgttttac aaaaaagctt
    11161 catcaagaca actatatttt agctgagttg ccaaaagaat ataaagagcc aaaaaatctt
    11221 cctcaacaag gaactacaaa aaaaaataat tacgattttt taaaatccag tcaaatttac
    11281 aataataaag aaatgagaca acaacgagaa aaaacaattg cgcaagagct taatcttagt
    11341 aggtttgagg atcttcctta ataaatctac tcagcgtatg cagatgacaa aggagttaag
    11401 atgtcaaaaa cgtatcattt tatcggaatt aaagggtcag gaatgagtgc cttggccctt
    11461 atgttgcatc aaatggggca taaggtgcaa ggtagtgatg ttgaaaaata ctatttcact
    11521 caaagaggat tggaacaagc gggcatcact atcttacctt ttagcgagga caacattacg
    11581 ccagatatgg aacttatcgt tgggaatgcc tttcgtgaaa acaacaaaga agtggcctat
    11641 gctttgagac accagattcc tttcaaacgt tatcatgact ttttaggtga ttttatgaaa
    11701 tcatttatca gttttgcagt agcaggtgct catggtaaaa cttctacaac gggtttattg
    11761 tcacatgtct tgaaaaatat cacagacaca tcttatttaa ttggtgatgg tactgggcgt
    11821 ggatctgcta atgcacagta ttttgttttt gaatcagacg aatacgaacg ccattttatg
    11881 ccttatcacc cagaatactc tatcattact aatattgatt ttgaccatcc tgattatttc
    11941 acaggaattg ctgatgtgcg aaacgctttc aacgattatg ctaagcaggt caagaaagct
    12001 ttgtttgttt atggtgaaga tgacgaacta aaaaagatcg aagcacctgc acctatttac
    12061 tattatggtt ttgaagaggg caatgatttt atcgcttacg acattacgcg tactacgaac
    12121 ggatcggact ttaaggtaaa acaccaagga gaggtcattg gtcagtttca cgtacctgct
    12181 tatggaaaac ataacatttt aaacgctaca gcagtaattg caaacttgtt tgtagccggc
    12241 attgatatgg ctctagtggc agaccatctt aaaacttttt caggtgtgaa acgtcgtttt
    12301 acagagaaaa ttatcaatga cactattatt attgatgact ttgctcatca tccaaccgaa
    12361 attgtggcaa caattgatgc ggctagacaa aaatacccaa gtaaagaaat tgtagcaata
    12421 tttcagccac atacttttac tcgcactatt gccttattag aggattttgc ttgtgcttta
    12481 aatgaagctg acagtgttta tttagcccag atttatggtt ctgcacgtga agtagataaa
    12541 ggtgaagtta aggttgaaga tttggctgct aagattatca aaccatcaca agtagtaacg
    12601 gtggaaaatg tctctcctct tcttgatcac gataatgctg tttatgtttt tatgggagct
    12661 ggagatattc agctatatga acattctttt gaggaattac ttgcaaattt aactaaaaat
    12721 aaccaataac atgtcctgcc ttaggcaggt tttctcattc taaaggagtt tgatatgttg
    12781 attagacagg ttcaaggatc cgatttggaa gttattgcta ccattgaaag tgacaatttt
    12841 tcaccacaag aagcaacgac aagagcagtt cttgaagagc gcatacggct gataccagat
    12901 acttttttag tggcacttat cgatcaggaa attgttggct atatcgaagg tccagttgtc
    12961 actactccta ttttggagga ttctttgttc cacggggtga ctaaaaatcc taaaactgga
    13021 ggctatattg ctattactag tttgtccata gctaaacatt ttcagcaaca aggggttggg
    13081 acagctttgt tggcggcact aaaagattta gtagtagcgc agcaaagaac aggtcttatt
    13141 ttgacttgtc atgattactt gatttcctat tatgaaatga atgggtttat taaccaagga
    13201 atttcagagt ctcaacatgg tggaaccctt tggtaccaaa tgatttggaa actttagaat
    13261 cacagctgat tcaactgttg gtttgttaaa aataagtaac aaatgcatca tttaattgca
    13321 attaggatac ttttaagtta taatggcggt tgattatatt taggaggatt ggctttgacc
    13381 gattttaagg ataaagacca acaagatcag cagcgaagct ttaaagagca gattcttgct
    13441 gagttggaaa aagcaaatca aatcagaaaa gaaaaagagg aagaactttt tcaaaaagag
    13501 ttggaagcta aagaagcagc taagagaacg gcccagctat atgctgaata taaaaggcaa
    13561 gatgcttttc aaaaagagtc tatagcacat aacaataaga cagctaaaca ctttcaagct
    13621 ataaaaggtg cggtaatgac ttcagaagcg cttaaaccga ctttactttc tgaaaaagaa
    13681 aactcatctt taaaaacgac aaataagaga gtcgtgcagg caaatgagct tcaagagact
    13741 gcctctaaag aatctcaagt accgttaact attgagaaag gtcattcagt gagacgaaaa
    13801 ttaagcaaac gccaacagac tgagcgagct gctaaaaaga tttcaaccgt tttgattagt
    13861 tctattatta taaccctttt ggctgttact ctagcaggag caggctatgt ttatagcgct
    13921 ttaaatcctg ttgataaaaa tagtgatgcc tttgttcaag ttgagatacc atctgggtca
    13981 ggcaataaat tgattggtca aattcttcaa aaaaaaggtt taatcaagaa tagcactgtt
    14041 tttagttttt atacaaaatt taaaaacttt acaaattttc agagcgggta ttacaatctg
    14101 caaaaaagta tgagtctaga agaaattgct agtgctttac aagaaggtgg tacagcagaa
    14161 cctaccaagc catctcttgg gaagatcttg attccagaag gatacacgat taaacaaata
    14221 gctaaagctg ttgagcataa tagcaaggga aagaccaaaa aagctaaaac accttttaac
    14281 gagaaggatt ttttggattt agtcacagat gaggctttta ttcaagatat ggtaaaaaga
    14341 tatccaaaat tattagcaac tatcccaact aaagaaaaag ctatttaccg tttggaaggt
    14401 taccttttcc cagcaaccta taactattac aaagaaacta ccatgagaga acttgtagag
    14461 gacatgctgg cagctatgga tgctactttg gtaccctatt atgataaaat tgctgctagt
    14521 ggtaagacag tcaacgaggt attgaccttg gcctctttgg ttgaaaaaga aggttcaaca
    14581 gacgatgaca gacgtcaaat tgcaagtgtc ttttataacc gccttaatag cggaatggca
    14641 ctacaatcta atatagctat tttgtatgcg atggggaaac ttggtgagaa aacaaccttg
    14701 gctgaggatg ctactattga caccaccatt aattctcctt ataatattta taccaataca
    14761 ggtctgatgc ctggtccagt tgctagctcg ggggtttctg caattgaagc aaccctaaat
    14821 ccagcctcaa cggattattt atactttgtg gccaatgtcc atactggtga agtttactat
    14881 gcaaaaacat ttgaagaaca ctctgcaaat gttgaaaaat atgtgaatag tcaaattcag
    14941 taaacacaaa catggtgctt aagcaccatg tttgtgtttc tgattcgttc aaaaaagaaa
    15001 agagagaaaa caattatggc agaaaaaacg tatccgatga ccctaacgga aaaagaacaa
    15061 cttgaaaaag aacttgaaga attaaaactt gtgcgtcgtc cagaaatagt ggagcgtatt
    15121 aaaattgcac gttcgtatgg agatctttca gagaactctg aatacgatgc cgcaaaagat
    15181 gagcaagctt ttgttgaagg tcaaatttca acgcttgaaa ctaagattcg ctatgctgaa
    15241 atcattgaca gtgatgctgt tgcaaaagat gaagtcgcta tcggaaaaac agttatcgtt
    15301 caagaagttg gaacaacaga taaagatacc tatcatattg taggtgctgc tggagcagat
    15361 atcttctcag gaaaaatttc aaatgaaagc ccgattgctc aagctttgat tggtaaaaaa
    15421 acaggtgaca aggttagaat tgaatcacca gcagctactt atgatgttga aatcatcagc
    15481 gttgaaaaga caaattaatc agatgaagcc attatttgcc taaccactaa gtgttttggt
    15541 tgctaatggc ttttctagta tcttcaaatg aggttgactg ttaggaaggt caagtattaa
    15601 tcgcattgat tatgagccaa gaggactata gatcaaagat cgttttcgat ggttatttaa
    15661 tgccaaaaga aaagaccagc accttaatgt actgacccca aaaagttgga cactatattt
    15721 taaggaagtg tatgatctaa ggaaactgac actaatgatt gaccgttttg caagacttta
    15781 ttaatgattt cttgctttat tttaaatgaa taagtctgat acgtttcctt ccttaacact
    15841 atcgactcca tactgaccca tcagtcaaac ggtatatctc aaacgactac ggtttactcc
    15901 aaattgattg gctagttgag gccaagaaac acctgatttt ttttaatgct aaatttctag
    15961 cttatcttca tagcttaatt tcatacaaaa acaccccaat cgttagattt ttttgatatg
    16021 tcccctttca ctagatgagt ccagtaaaag ggggcatatc gttttttgtc taacttttgg
    16081 ggtgcagttc ataattgtgc taggtttttt gtctttctta acgctttcgc tgttttccag
    16141 cattacgatt ggattttatt tttttaggca agttagcctg ttccatgttt tgtgaaggcg
    16201 taacgtcctt acgagaagaa gttgattggt atgctttagg cgggttttta gcgtattctt
    16261 ctttaatttg cttatgcaat cgaggtttta agaggtaggc tgtgattaac tgttgaataa
    16321 tactaaagaa accaccaact agccagtaaa gaccaacacc agcagggagt gagaaggaca
    16381 tgaagatcat catgataggc atggtgtaca tcatcgtctt catttgctca cgttgttctt
    16441 ctgaaacggc catcattgat aaccatgatt ggaagaagta aagagcagcg ataattgctg
    16501 ttaacactaa actacggcta ccaagatcaa tacccataaa ggtgcttgtt gatactcctt
    16561 tagtgtattg agcggcaaaa tacatggcag aaaagaatgg catctggatg agaagaggta
    16621 gacatccaat acctccaaga ggattaatcc catgagcacg ctgagcagcc attaattctg
    16681 tttgggcagc cattttttct tcctgactac ttgcctgttt gatacgtttg ttgattggtt
    16741 caaaaacagg tttcaagaag gccattttct cagattgata acttgctttc caagattgat
    16801 atagacctag tggtaagata agtgttctaa caataatggt tacgatgatg atagccagtc
    16861 cataacctag tccagcattg tttgcaaagt aatcgataaa gtatgacatg ggttttccaa
    16921 gaaattccca aatcatacct ttgggatttc cgtgagcgtc tcttcccaca cagcctgtca
    16981 aggtgaatag aattgacagt gctagtcctg aaaagaggat gcgatttaat gttagtttca
    17041 atgtgtttat cctttaattt taatatactt atctatttta cagttttttt cagaaattca
    17101 caatagttct gaaggccgat ttttacttgc ctatttgaaa gtcctgaaag tcttcaaaat
    17161 tagccatggt gacatccaca taagtgactt ttgcccattt ggatggtcct ttacgaactt
    17221 cttgaataaa tgtggctatt ttatctgaat cttttgattg ggccaggatt tcaacggtcc
    17281 catcgttgtt attccagaca cgaccataaa tatctcctat atctagagct aaagtatggg
    17341 tagcgtatcg aaaaccaaca ccttgtacac gtccagaaac aattaaacgt actttttgca
    17401 taagaagggc ctccttgctt ttattatagc acatacttag caggaaatct ctttagctaa
    17461 ttgtctactt cctgtcactt ttactaaaga caagaataca aaaatgagat agttagtagt
    17521 actaacagta aaagaggttt gtctatttta aaaatagtat caaaaaaagg tcttttaggt
    17581 ttaaaacatt gaactgcccc caaattgacc atctaagaag ttgatgcttt caaatgctgt
    17641 cgcgtatggt ataataggcg catggttatt acatcaaaaa caaatcaact aattaagcaa
    17701 acaaaaaaat tattgcaaaa aaagcatcga aaacagtcct atttgattga gggctggcac
    17761 ctttttgaag aagcccaaaa atcaggacaa gtttttcgcc acatctttgt tttagaggaa
    17821 atggtggagc gtctagcagg tgagcaagaa ctcgttattg ttagcccaca agtgctcaag
    17881 gagttgaccg actctcctag tccacaaggc attgttgctg aggtggagat acctaagtta
    17941 gcctttccat cagattacaa agggaaatat ttggttttag aggacgtcca agacccagga
    18001 aatcttggga ccatcattcg tacagcagac gctgcacggt ttgatggggt ctttctttct
    18061 gaaaaatcag cagatattta caaccaaaaa accctacgtt ccatgcaagg tagccatttt
    18121 catttaccaa tttggcgaac agatgtctat cagctttgtc gagagctcca agagtataaa
    18181 actcccatcc tagcaacaac tctgtcaaag aaatctgttg actataaaag cctcacacat
    18241 catgaaaggt tggctttggt gttgggaaat gaaggccagg gcattagtgc tgaaatggca
    18301 gccctagcag accaactggt tcatattaca atgcctggtc aggcggaaag tttgaatgtt
    18361 gccgtagcag caggaatcct cattttcagc ttgatttaag ctaatatgat ataataacca
    18421 agtgaggtaa ttatgttatg cattatacag aagataaaga atatatggaa cacgtcggtc
    18481 atttgattgc tcatcctcgc tttcaaaaat tgagtcatat tgttcagcac caacattcaa
    18541 cgcgtcttga acattctatc aatgtttctt attccagtta taaattagcc aagcgatttg
    18601 gttgggatgc taaaagtaca gccagagggg gcttacttca tgatttcttt tattatgatt
    18661 ggcgcgtgac caagtttaat aagggtcatg cctgggttca tcctcgtatt gctgtacgaa
    18721 atgcgaaaaa gttaacagaa ctcaataaaa aagaagagga tatcattcta aaacacatgt
    18781 ggggagcgac cattgctttt ccgcgttaca aggaaagtta tattgtgaca atggtagata
    18841 aatactgggc tgtcaaggaa gcagtgacgc ctttgagaca aaaatggtct aatcgacggt
    18901 ttcttcgccg caagaccctc caaagtcata atcgttaagt aaaggagtta ttatgaacga
    18961 tcacgttatt tatacacaat cagatgtggg attaaatcag ttttttgcta aaatttatag
    19021 tctagtggga atgggagtgg gtttgtctgc ttttgtttct tatttgatgc tttatccatt
    19081 tagagaaaat ttaatctcta ttcttgttaa tcagccgatg atttactatg gtgctgcgat
    19141 tattgaactt attttagttt ttgtagccag tggtgcagca cgaaaaaaca caccagctgc
    19201 attgcccatt tttcttatct attctgcgct aaatggcttt accctaagct ttattattgt
    19261 ggcatacgct caaacgacag tttttcaagc atttctttct tcagcagctg tgttttttgc
    19321 catgtcaatc attggtgtca agactaaacg ggatatgtca ggtcttagaa aggctatgtt
    19381 tgctgctttg attggtgttg ttgtggctag tctgattaat ctttttattg gtagtgggat
    19441 gatgagttat gtcattagtg tgatttccgt gctaattttc tcaggtctca tcgcatcaga
    19501 taatcaaatg attaagcgcg tttatcaagc aacaaatggc caggttggtg atggctgggc
    19561 tgttgctatg gccttaagct tgtatttaga ttttattaac ttatttatca gccttcttcg
    19621 tatttttgga agaaatgatt aacattaaaa agtgagtata actactcact ttttaatgtt
    19681 aacaggataa gtaaactgta aggaagaaat tcgagataga tataagatga ggctgctaaa
    19741 agacctagtg ttaatttgta aactctttct ttttttggta tactgttaag gattaaatac
    19801 gaggagaata tagatagata tgtcaacagc tatttggatt ttattactta ttgttgccct
    19861 tggggtaggt gtttttggtg ggatttttat tgcccgtaaa caaattgaaa aagaaattgg
    19921 tgaacaccca cgtttaacac cagaagctat ccgtgaaatg atgagccaga tggggcaaaa
    19981 accaagtgaa gcaaaaatcc aacaaactta tcgtaatatc atcaaacaat caaaagcagc
    20041 cgtatccaaa ggcaaaaaat aaaagctaat tgttataggg gaaacctttg tgatagttag
    20101 aagtgaacag tctttgatct caatgttcag tagcacttgt cttatcagtt agatttgtca
    20161 actgttcagg gtttaaaaag aaactcagtt ggctatttcc aactgagttt ttcatatcgt
    20221 cagtaaggaa gtaaacataa tggatacaag accaattggg tttttagatt ctggtgtggg
    20281 aggtctgacc gtcgtttgtg aattgattcg tcagctccct catgagaaga ttgtatatat
    20341 tggtgattca gcaagagccc cctatggtcc tagacccaaa aaacaaatta aggagtatac
    20401 ttgggagcta gtcaattttt tgctgactca aaatgttaaa atgattgttt ttgcatgcaa
    20461 taccgcaaca gcggtggctt gggaagaagt aaaagcagct ttagatattc ctgttttagg
    20521 ggttgtctta ccgggggcaa gcgcagctat taaatcaacg acaaaaggcc aggttggggt
    20581 catcggaacc ccaatgacag tggcttcaga catttatcgc aaaaaaatcc agctattagc
    20641 accatctatt caagtaagga gtcttgcttg cccgaagttt gtaccgattg tggaatcaaa
    20701 tgagatgtgt tcgagtatag ctaaaaaaat agtttatgac agtctatcac cattagtcgg
    20761 taaaatagat acccttgtac taggatgtac tcactatccc ttgttacgac caattatcca
    20821 aaatgttatg ggaccatctg ttaagctgat tgacagtgga gcagaatgcg tccgagatat
    20881 ctctgtctta ttaaactatt ttgacattaa cggtaattat catcaaaaag ctgtcgaaca
    20941 ccgctttttt acaacagcaa atcctgagat ttttcaagaa attgcctcga tttggctgaa
    21001 acaaaaaata aatgtggagc atgtcacctt atgagtgaaa aaatttacga atacaaagat
    21061 gaaaataact ggtttatcgg aaagatgact ggtcataatt taatatcggg ctggggagtc
    21121 aaacacacga caatcaaaaa aatagatgac cttttagacg gtattgccgc aacgttagat
    21181 tgggaaaatc ctaaaggtta cgatgtttcg gtggtgcgtt atcagtctcc tttatctctc
    21241 atcaccttta ttatcgacat gataaatcaa gagacccagc gtaaggttaa ggtaactccc
    21301 catgcaggca cgattttatt aatggaaaat gcgaaactgc ttgcagttta tctcccagaa
    21361 ggtggagttt caacggctac attttttgcg actagtgaac agagatttgg ggatactgtt
    21421 ttaatcgcta cacgaaacga gggaaaaacc aaagagttcc gcaatctgtt tggccagcta
    21481 ggttatcgtg tggaaaatct caatgattat ccagaattgc cagaagtagc agaaactggt
    21541 actacttttg aagaaaatgc tcgcttgaaa gcagaaacga tttcacgcct cacaggaaaa
    21601 atggtattag cagacgattc aggactaaaa gttgatgcct tgggtggttt acctggggtt
    21661 tggtcagctc gtttctcagg accagatgcc acagatgcca agaataatac caaactctta
    21721 cacgaattag ctatggtgtt tgaccaaaag aagcgctcag ctcaatttca tacgactttg
    21781 gtagtggctg ctcccaataa agatagtttg gttgtggaag ctgagtggcc aggctatatt
    21841 gctacgcagc ctaagggaga aaatggtttt gggtatgacc ctgtctttat cgtcggagag
    21901 actgggcgtc atgctgcaga attagaggct gaccaaaaga atcagttgtc gcatagaggt
    21961 caagcagttc gaaaattaat ggaggtcttt ccagcatggc aagcgaaaca atcatagtaa
    22021 tgagtgactc tcatggtgac cgagatatcg ttcaagccat taaagacaag tatcttggtc
    22081 aagttgatgc tattttccat aatggggatt ctgaattaaa cagctcagat cctatttggg
    22141 ctggtattta tgtcgttggc ggtaattgtg actatgatac aggttaccct gatcgtttag
    22201 taactcaatt aggcacagtt acgattgccc agacacatgg tcacttgtac catattaatt
    22261 tcacttggga taaactggac tactttgctc aagaagtagc agcagatatt tgtctatatg
    22321 gacacctaca ccgtccagct gcttggcaag ttgggcaaac cctctttatg aatcccggat
    22381 cagtaacaca acctcgcgga gaaattaacg agaaattata tgctcgtgtt gaactaactg
    22441 atactcaaat taaagttgat tactttacaa gagatcataa actttaccca tcactgtcta
    22501 aggaatttaa acgatgatcg caaaagaatt tgaaacgttt ttaatgagtc atttagataa
    22561 ttatcttatc ccggaacaag acttggctat ttttatcgac acacataatg cagaccatgt
    22621 gatgttacta ttggtgagta atggtttttc tagagttcct gttatcacac gggagaaaaa
    22681 atatgtggga accattagca tctcagatat tatgatgtac caatctaaaa ggcagctgac
    22741 agattgggaa atgagtcaaa cagatattgg cgagatggtt aataccaaaa ttgaaaccat
    22801 tagcattact tctagcctaa cagaaattat gcacaagtta attgatttcc cattcttacc
    22861 ggttgtagat agagcgaatc gctttgttgg gattattacg cgaaaatcta tattaaaggc
    22921 tgtcaacagt cttttacatg attttacaga tgattacacg attataaaaa aatgaagagt
    22981 tatattgaac cttttattgc tagcaaggct ttatctcaaa atagtcaaaa agcttatcga
    23041 tacgatttgc aacagttttg tcaactggtt ggtgaacgtg tcaatcaaga caaactatta
    23101 ctttatcaaa atagcatagc taacctaagt ttatctgcaa aaaaacgcaa attatcaaca
    23161 gctaatcaat tcttatatta cttgtatcaa ataaagtatt taaatagcta ttttcgatta
    23221 acagatacca tgaaagtgat gcggactgag aagcagcaag ctgctattat taatactgat
    23281 attttctatc aaaaaacccc ttttgtgtgg gggcaattaa tcagcttatt aatattggag
    23341 ctgggtttaa ctccgagtga agtagctggt attgaagtag ctaacttgga tttaaatttt
    23401 caaatgttga cgctaaagac taaaaaaggt gtgcgtgtat tacctttgtc gcaaatacta
    23461 atcccttttt tagaacaaca actcgttggc aaagaagtct atctttttga acatcgaggc
    23521 atcccttttt caagacaatg gttttttaat cacctaaaaa ccttcgttcg aagtattggt
    23581 tatgaaggat taactgctca aaaattacga gagcaattta ttttgaaaga aaaactagct
    23641 ggtaaatcga taatagaact tagcgatatt cttgggctga aaagccctgt gaccttagaa
    23701 aaatactaca aatcataatg gatataaaac ttaaagattt tgaaggtcct cttgatttac
    23761 tcttgcactt ggtatcacag tacaaagttg acatctacga ggttcctatt gttgaggtta
    23821 ttgagcagta ccttaattat attgaaacct tacaggtaat gaaacttgaa gtggctggtg
    23881 actacatgct gatggcaagt cagctcatgt taatcaaaag tagaaggcta ttaccaaagg
    23941 tggtagaaca catcgaagaa gaagatttag aacaagatct gttagaaaag atcgaagaat
    24001 atagtcgctt taaagcagtt agtcaagcgt tagcgaagca acatgaccaa agggcaaaat
    24061 ggtattcaaa acctaaacaa gaattaattt ttgaagatgc tatcttacaa gaagataaaa
    24121 cggtgatgga tctatttcta gctttttcaa acataatggc agctaaaaga gcagttttaa
    24181 aaaccaacca tacagtcatc gagcctgatg attataagat tgaagacatg atggcttcta
    24241 ttaaacagcg gctggaaaaa gaaaacgtta ttagcttatc agcgattttt gaggaatgcc
    24301 aaacattaaa tgaagttatc agtatatttt tagctagtct agaattgatt aagcttcacg
    24361 tggtatttgt agagcagctc tctaattttg gggctatcat actacgaaag gagaagaaat
    24421 gacgtatttg tcacagatag aagcgctgct atttgttgca ggtgaagaag gattaagttt
    24481 gcgacatcta gcgtctatgt tatcattaac gccaacagct cttcaacagc aattagaaaa
    24541 actctctcaa aaatatgaaa aagatcaaca ttctagtctt tgcttgatag aaacagctaa
    24601 tacctacagg ctagtaacta aagaaggttt tgcagaactc ttgcgtgctt atgccaaaac
    24661 accaatgaac caaagtttat ctcgtgcgag tttagaggtc ttgtctattg ttgcatacaa
    24721 acaacctatt acacgaatag aaattgatga cattcgaggg gtaaactcca gtggagcttt
    24781 gagtaaatta ttggcatttg atctcattag agaagctggc aaaaaagatg tggttgggcg
    24841 tcctcatttg tatgcgacaa cagattattt tctggactat atgggaatta atcatttaga
    24901 cgaattgatt gaagtatctg ccgttgaacc ggcagatgaa gagattgctc tttttaggac
    24961 acaagactga gttttgcttg tcatcaaata gcaaatgacg tttacaaaaa gatttgtttt
    25021 aaggcgtttt agttgaaaaa agccttaaac tagggacaac aaaaagttgc tcaaaagaaa
    25081 gaagaatata atgcgaatta acaaatacat tgcccatgcg ggtattgcca gtcgtcgtaa
    25141 ggctgaagaa ttgattaagc aaggcttagt aaccttaaat ggtcaggtta ttacagattt
    25201 agcaaccact gtaaaatcgg gagacgtggt tgaaatagaa ggaagtccta tttacaacga
    25261 ggaaaaggtt tactatttgc ttaataaacc tcgtggcgct atctcaagtg tgtcagatga
    25321 caaaggccgt aaaacggtac tgggtttact acctcaggtt aaagaacgca tataccctgt
    25381 tggacgattg gattgggaca catcaggggt ccttatttta acgaatgatg gtgattttac
    25441 agatactatg attcatccta gaaatgaaat agacaaggtt tatctggcgc gtgtcaaagg
    25501 aattgcaacg aaagaaaatc ttcgtccctt aactcgagga attgttattg atggtaaaaa
    25561 gaccaaacca gctcgctaca atattgttcg tgtagaagct gataagaatc gatcgatcgt
    25621 agaattgact atccatgaag ggcgaaacca tcaggttaaa aaaatgtttg agtctgtggg
    25681 gcttttggtt gataaattat ctcgtacacg ttttgggacc gtcgacttaa agggactaag
    25741 accaggagaa gcaagacgct taaataaaaa agaaataagt caactgcata acttagctaa
    25801 tacgaaaaaa tgatgaaaaa actattaata gtatctgtta aagcttatca aaaatacatt
    25861 tctcctttat ctcctcctag ttgtcgctac aagccaacct gttcagctta tatgttaact
    25921 gctattgaaa aacatggtac taagggaata ttaatgggga tagcaagaat attaagatgt
    25981 cacccttttg ttgctggtgg tgtcgatcct gttccggaag attttagcct aatgagaaat
    26041 aaaaacacgt ctaaaaacgc tgaaaaggca tgactttatc aaaaaatggc ttgacattga
    26101 tagctgaagt gttataatgg aaaacaagca ttcgttggtt taaatcaaac ctgttatgat
    26161 ttaagttagc gagtcaccat caaccacatt gtttgctgag catgactccg agcagtgtgg
    26221 ttttttaaat taaaaagagt aaatgagatt atgacgacaa aagaacttat caacaaaaac
    26281 gacaaggtaa aaaaggctcg caatcatatt gtcctgtttc aaccccaaat tccacaaaat
    26341 actggtaatg ttgctagaac atgtgctgca actaatgcac ctttacacat tattaaaccc
    26401 atgggatttc ctattgatga ccgcaaaatg aagcgtgccg gcttagatta ctgggacaag
    26461 ttagaactgc atttttatga tcatttagaa cagtttatca atcaatgtca cggacagctt
    26521 cacctgatta gcaagtttgc agttaataat tattctcaag ctacttatgc tgatggcgat
    26581 tcccattatt tcttgtttgg acgagaagat acaggcctgc cagaagattt tatgcgagaa
    26641 catgctgaaa aagcacttcg tattccgatg aatgatgagc atgtacggag tttaaatgtc
    26701 tcaaatacgg tttgtatggt gatttatgaa gcccttagac aacagggctt tcaagggtta
    26761 gagttaaagc atacttacga acacgataaa ctaaaataat ggacttttta aaagcgtcag
    26821 tttttggcaa catgcgttgt cagtagctga cgcttttaaa aatttagtca aagctattgc
    26881 tatctaggtt taaatctgct atgctagaag tgtcttcagg gcagggtgtg attcccgacc
    26941 ggcggtgact agtaactaga agtccgcgag cgcaagctga tgtggtgtaa ctccacaacc
    27001 gacagtatag tctggatgag agaagaccgg gctttttgtg taggcttatt ttagctacac
    27061 aataacgacg gaacttcttt caatttgaaa aaattggagg aattttttaa tgtcaaaaac
    27121 acataaaatg attatgattg gtatactttc tgcgatatca tttctattga tgctagttag
    27181 ctttgccatt attccaggcg cagctttttt aaaaattgaa tttagtatta ttcctgtcct
    27241 atttggttta atgattatgg acttaaagag tgcttacttg attttgttat tgaggtcctt
    27301 attgaaactc tttttgaaca atcgtggggt taatgatttt attggtctcc caatgaatat
    27361 tatcgctatc gctctatttg tgacggcttt tgccttagtt tggaaccgtc aaaaaacgct
    27421 tagtcaatat gtatttgcta gcttactagg cacagggtta ttaacatttg gcatggttgt
    27481 tctcaattat acttttgcca ttcctttata tgctatattt gcaaatattg atatcagagc
    27541 ttatattggt gtcactaagt atatgatgac tatggttatt ccgtttaatc ttgttgaagg
    27601 gttgatattt gcaattacct tttattttgt gtatattgca agtaaaccaa ttttagaaag
    27661 atacttacac taatatgatg acaaacaaac aaactcactt tttaatagct tcttttgcat
    27721 tactaatctt tgttataata ggctatacgg tcaaattttt tcctgaacgt ttggcacttc
    27781 tagataatac tattcaagca gagattagag gcaaccttcc tatagtgctg acgcaattct
    27841 ttaggggtgt tactgtattt ggaaatgtca tgacacaagt tttgttagtt attgtgtcgg
    27901 tcctagtttt attctttatg aagtggaaaa ttgaagcctt gtttatctta tcaaatggtg
    27961 ccattgcagc atttttgatt acgactctca agctctttta ccaaagacca agacctgcta
    28021 ttgagcattt ggtttatgct ggtggctatt cgtttcccag tggccatgcg atgggtagca
    28081 tgctgatttt tggtagccta ttgattattt gttatcaaag gctacattca aaactattac
    28141 aatttgtgac tagcatgatc tttataatac taattttagt aattgggtta tctcgtattt
    28201 atctaggggt acactaccca agtgatattt tagcaggctt tgtattagga tttgggattt
    28261 tgcagtttat ttatcctttt tataagcaaa aacgatttga atggcgattt cttttgaaac
    28321 aagactaatt tttcttgaga agacataggg cttatttaaa tcatttagaa taacattggc
    28381 aagcatcacc ccttgattgc cagtgttatt ttagttttag ttagatgatg tgagctgatg
    28441 attagtaaat acgtttagat aaaaaatgat taaagtgcta tgtaacgtca gaaaagacgt
    28501 gatttttgtt gatctgtaat cattaagtaa gctgatgtag aagtagtgaa ttctaatgac
    28561 tgagctatct tttgcataga tgactatctt gctagtaatg atttgtgcta caatagaaac
    28621 atgaaaaaaa gatatcagac attaaacgag cattaccgtc aactatttgg cgcaaaaatg
    28681 tttaaagttc cgattgatgc cggctttgat tgtcccaatc gtgatggaac ggtagctcat
    28741 ggaggatgta ctttttgcac agtatcaggt tctggagatg ctattgtagc accagatgct
    28801 ccaattaaag agcaatttta taaagaaatt gattttatgc accgtaaatg gcctgatgtc
    28861 aatcgctatt tggtttattt tcaaaacttt accaatactc atgatacagt tgatgtgatt
    28921 cgggaccgtt acgaacaagc tattaacgaa cctggagttg ttggtattaa tattggcaca
    28981 cggcctgact gtctaccaga tgataccatt gcttacttag cagaattatc agaacgcatg
    29041 catgtgacag tggagctagg cttacagaca acttatgaag aaacttctag gctaattaac
    29101 agagcacatt cttatgattt gtataaagaa acggtgaggc gactaaggca ttaccccaac
    29161 attaatattg tctcgcactt gattaatgga ttgccaaaag aaacccatga catgatgctt
    29221 gaaaatgtga gacgctgtgt aacagacaat gacattcaag gaattaaatt gcatttactg
    29281 catttgatga ccaatactag aatgcaaaga gattaccatg aaggacgctt aaagttgttg
    29341 agtcaaaagg actatgtttc gattatttgt gaccagctag agattatccc taagcatatt
    29401 gtgattcacc gtattacagg agatgcccca cgagatatgt tgattggacc catgtggagt
    29461 ctaaataagt gggaagtatt gaatgccatt gataaagaaa tggaaagacg aggaagcttt
    29521 cagggctgta aagttgacta atttgtctca ttttgacgga tttggatagg agaaaagtga
    29581 tgctaaaacg ccccattcat ctctcgcatg attttttagc ggaggttgtg gataagtcta
    29641 gtgtggttgt tgatgccacg atgggtaatg gaaatgatac tgcctttttg gcacaattag
    29701 ctaaaaaggt ttatgctttt gacgtgcaag aacaagctat tagaaaaaca agtgagcggt
    29761 tagcgcagct aggattatcg aacgctgagc tgattttagc gggtcatgaa gcagttgatc
    29821 agtatgtgac ggagccggtg agtgcagcga tttttaacct tggttatttg cccagcgcag
    29881 ataaatctat tattacctta ccaaatacca cactccaagc tttatcaaaa ctactaacgt
    29941 tattaatggt aggtgggcgt attgctatta tggtctatta tgggcatgat ggaggcagtt
    30001 tggaaaaaga tgctttatta gattttgtca agcaattaga ccaaagaaag gtttctgcaa
    30061 tgctttatca gcctcttaat caggtgaata ccccaccttt tttaatcatg ttggagaagt
    30121 tagcagacgt ttatccctta tccctgtgtc aagtctacta gaagttctgc gattatttcg
    30181 gtggaaaaag aagaaatccc ataggctttg gctataaatg tgatataatg aagtcactat
    30241 attatttttt taggagttac attaacatgg aagaccctgt gagtcagccc ttagtgattc
    30301 aatttttatt gttagttgtt ttaaccttgt taaatgcttt tttttcagcc agtgaaatgg
    30361 ccttagtttc tctcaatcgt tctcgggtgg aacaaaaagc agcagacggt gataaaaaat
    30421 acgctcgttt gttgcgggtt ttagaggaac ctaatcattt tttatcaacg attcaagttg
    30481 ggattacctt tattagttta ctatcaggag caagtttatc agcttctttg ggtaaggtga
    30541 tctcaggttg gctaggtaat tcagcgactg caaggacagc tggtactatc atctccttgg
    30601 ttttcttgac ttatgtctct attgttttag gagaattgta tccaaaacgg attgccatga
    30661 acctcaaaga caagttggcg atcgtttcag cccctattat cattgggttg gggagactgg
    30721 ttagtccttt tgtgtggctc ttatctgctt ctactaattt gctgagccga cttaccccaa
    30781 tgacctttga tgatgcagat gagcaaatga cacgtgatga aatcgagtat atgttatcaa
    30841 aaagtgaggc gacccttgat gctgaagaaa ttgagatgtt gcaaggagtt ttctcacttg
    30901 atgaaatgat ggcgcgtgaa gtcatggtcc caaggaccga tgctttcatg attgacatta
    30961 acgatgatcc gcttgaaaat attcaggaaa tcttaaaaca aagtttttca cgcattcctg
    31021 tttatgatgt ggataaagat aaaattatcg gtctcatcca cactaagcgt ctcttggagt
    31081 caggtttccg ccagggattt gatcagatta acatgcgaaa aatgttacaa gaacctcttt
    31141 ttgttcccga aaccattttt gtagatgatc tcttacgcca gctgcgcaat acccaaaatc
    31201 agatggctat tttgctagat gaatatggtg gtgtggcagg acttgtgact ttggaagact
    31261 tgcttgaaga aatcgtcggt gaaatcgatg atgaaaccga taaagcagaa caatttgttc
    31321 atgagattgg agacaatacc tatattgttg ttggtaccat gactttaaat gagtttaatg
    31381 actattttga taccgaacta gaatcagatg atgtagatac cattgctggt ttttatttga
    31441 caggtatcgg aaccattcca agccaggagc aaaaagaagc ctacgaaata gataacaaag
    31501 acaaacattt agttctaatc aacgataaag tcaaagatgg ccgtattacg aaattaaaat
    31561 taatcctgtc taatatagaa cagattattg aggaagacta ggctccatgc ctagtctttt
    31621 tgatgaaaag tgttataatg aaaatgtaaa aacgtttaca agtaatgaag gagatgcaat
    31681 gactgagaaa gattatggac aagtaacagg gatggtgcat tcgacagaaa gttttggttc
    31741 tgtagatggt ccaggcattc gttttatcat tttcttgcaa ggctgtaaat tacgttgcca
    31801 atattgccat aatccggata cttgggagat ggaaaccaac aattcaaaaa tcagaacagt
    31861 caatgatgtc ttgaaagaag cccttcagta caaacatttc tggggaaaaa aaggaggaat
    31921 taccgtatct ggtggtgaag ctatgcttca aattaatttt atcactgcct tattcatcga
    31981 agctaaaaaa ttaggtatcc ataccacact agacacctgt ggctttgcct atagaccgac
    32041 acctgaatac catcaggttc ttgataactt attggcagtg accgatttga ttttattgga
    32101 cttaaaagaa attgatgaaa aacaacataa aattgtcacg cgtcagccta ataaaaacat
    32161 attgcaattt gctaggtatt tatccgacaa gcagattcct gtctggattc gacatgtctt
    32221 ggttccaggc ttgactgata ttgatgatca tttgacacga ttgggtgagt ttgtcaaaac
    32281 cttgaaaaat gtcgataaat ttgaagtttt gccttaccat accatgggag aattcaagtg
    32341 gcgtgagcta ggtattcctt atcagttaga aggtgttaaa ccaccaacta aggaacgcgt
    32401 tcaaaatgct aaaaatctga tgcagaccga atcttacaca gaatatatga atcggattca
    32461 tcagtcataa gagctgttat agggttgagg tttcctcaac tttttaaagt attataagca
    32521 gatcttttgt aatcttcctt tgattttagg gggtaatttt gctaaaataa aatgaataaa
    32581 caaaatgagg tagaatgatt atgtcaaaaa ttttagtttt tggtcatcaa aatcctgata
    32641 cggatgctat tgcatcatct tatgcttttg attacttatc ccaaaaagcg tttggtttgg
    32701 atactgaagt cgtagcttta ggaacaccta acgaagaaac agcttttgcc cttgattatt
    32761 ttggtgttga ggcaccacgt gtggtagagt ctgctaaagc acaagggtct gaacaagtga
    32821 ttttgacaga ccacaatgaa ttccaacaat ccattgcaga tatccgtgag gttgaggtat
    32881 atggtgtggt tgatcaccac cgtgtggcta attttgaaac tgcaaatcct ttatacatgc
    32941 gtgtggaacc agtaggatca gcctcatcta ttgtttaccg tatgtttaaa gaaaatggca
    33001 ttgaggtgcc aaaagcgatt gcagggatgc tcttgtctgg tttgatttca gataccttgt
    33061 tgttgaaatc tccaacaact catgtgtctg atcaccttgt cgctgaagaa ttggcagagc
    33121 tagcagaagt gaacttggaa gactatggaa tggctctttt aaaagcaggt acaaaccttg
    33181 caagcaaatc agaagtagaa ttgattggta ttgatgccaa aacctttgaa ttaaatggca
    33241 atgctgtgcg cgtggcacaa gtgaacactg ttgacattgc agaagtctta gaacgtcaag
    33301 aagctattga agcagccatt aaagatgcca tggctgcaga aggttattca gattttgttt
    33361 tgatgatcac ggatattgtt aactcaaact ctgaaattct agctattgga gcaaacatgg
    33421 acaaggttga agcagccttt aacttcaccc ttgacaacaa tcatgccttc ttagcaggtg
    33481 ccgtttctcg taaaaaacaa gtggttccac aattgacaga aagttttgga gcttaatgaa
    33541 aagacgcttt aaaaggactt ggtttttttg accaagtcct tttttcactc ttctatgcta
    33601 taatagaagg tatgataaca atatttcatt cagataaact caccagacaa cctttctttc
    33661 aagaccttat caattatctc gaccagcatg attacgttat attacgagag atcaaaaaag
    33721 cctttcctaa tgtgacaggt attgacaagg ccatcgaaag ctatgttcaa gctggctata
    33781 ttcgccgtga aaataagcgt tatggcatca atcttccttt ggtgagttct gatcagcagc
    33841 tggccttaga cactatgctt tttgtggaca cctgttcagc tatgtatgaa aatattttag
    33901 cggttgtttt tgagactcag ctaacaaacc aaaccaatca cgtggtgatc aaggaaaaga
    33961 ccaacatcac gagagacgat ttgaccctgg ctaattattt ttaccgtctc aaaagaggtg
    34021 agaagccatc agctgagcag atggacttgt atgacctctt gggggatgtg aatcaggaat
    34081 atgcccttaa atatatgaca acttttttgc ttaaattcac gcgcaaagac cttgtgatgc
    34141 aaaaacgtcc tgatatattt gtggaagctc tggttacact tggttaccta aaacaagtgg
    34201 agcctacaac ataccagctg ctaatgacct tggataaaga aagtttaacc tttatagcac
    34261 catagaaaaa agctaggaac aaggactgtt cttagctttt tgagtgcttt tattttgttt
    34321 ttgccattaa gtaaagaaaa taaggggctc cgataagcga cactaaaatc cctgtctgga
    34381 tattactgcc aaccagatag actcgtccta ctgtatctgc aacaagaagt aggatcattc
    34441 cgattaaaat gctaatgggt attgttattt tatgattact ccctaccaag tatgtactaa
    34501 agtgtccagc tattaagccg ataaaactaa tattaccaac caaaagaaca ctcaaagcac
    34561 ctaagccagc tgctaagaca agtatcaaac gacgcttacg gtttaaaggg agtcctagcc
    34621 caatagcagt attatcagct aatcccataa tattaaggaa atgggcttga ctataagtca
    34681 atagccaaaa acacaataat aaaggagcga tgacactcag agtaggccag tcgtctccta
    34741 ttaattggcc gctaagccaa ttgatgacca aatcgacttt gtagcgatta atatgaccaa
    34801 caagagcaac cattagactt gaaagcatgg tagtaacagc tacaccagta agaattaacc
    34861 gtgtaggatc aatttgtcct tgttttgtca aagatagcca ataaacgcta aaggttgtta
    34921 caacagcacc aagcatggca aataaaggca agtaatggct gatatgagct tgctcaaaga
    34981 aagaaaagga gaccacaata gccaaagaga tacctgctcc tatagtaata cctaatacac
    35041 cagagtcagc taagggattt cgtgttaaac gttgtaataa tagccctgat aaagcaagag
    35101 aaccgcctcc tagaaaagca gctagaattc ttggcagacg aatgttaata acgatgaaag
    35161 atatggcatg gctagatttt cccaaaaaca catggacaag gtccaaaaaa gatagatgag
    35221 attctccaag ggacaaagct aatcccataa gacttacaag tagcaggatc aggctagtat
    35281 ataaccttaa aaggtgtcgg tgagatttca atagcggcct cctcttctta ttaaccataa
    35341 aaagcatgga aaaccaatta agctaaccaa tgccccaaga ggggtttcat aaggcggatt
    35401 gagattacga caagcaatat caacccatac catgaaacta gcgccagaaa ctgcacaaag
    35461 cggcaaaagg tatcggtaat gatgaggtgt aaagtgtttc ataagatgag gaatgactaa
    35521 gccgataaaa gaaatgcttc cagcaatggc tacagctgct gatgataaaa taagaaccaa
    35581 aatcataaag actgcactga tcaagttggt cttttgccca agagcttttg cctgagactc
    35641 acttagacta aggacggtca ggtgataaga taatagctgg gctaaacaaa gactaagaat
    35701 aataagagga gcaatatagc cgatcatttg ccaattgacc ccgacaagac ctcctgcttg
    35761 ccagccgata acagcatttg tcagatgata gtaattggta ataccttgac ctaatgctga
    35821 tagcagtatg gaaaccatag ctcctgctaa gacaaggcgg agctgatggt agcctttgcc
    35881 agattgataa gaaaggccaa agactaaggt tgctgccagg ctagacccta acaacgaaag
    35941 cagaataatg agggaatagt gcaagtgagg tacaaacgca taagccagta ccaaggctag
    36001 tcctgctcca gcattaatgc ctaaaaggcc aggctcagcg ataggattac gtgtgattgc
    36061 ttgcatgata gttccagaaa cagctaacgc agagccagtt aaggtagcac caaaaagtct
    36121 cggtaatcgt atagctagta aaacattggc tttatggccg ttttgctttc caaataaaat
    36181 agagttaaga tcctgatgtg ataaagcaac ggctccaaag cgcagatgac agtaaatagc
    36241 aacacataag ctaaaactaa gtagcacaaa gacaagccaa aagatatgtg acttagtgat
    36301 tatctgcgtt ttttttaagc aaaaacgctc gagacacaaa aagggatgct tagttttcac
    36361 ttgataagat tgcctctcgt aaagtctcta attgatattc taaagaaaga ggatcagtaa
    36421 agtaaaagac attggcattg actttgatca catggtgttt tttcacagct ggaatggatt
    36481 gccatagctt gctttcatat agagcagaac cagtcttatc atcttctgca gcaacgacga
    36541 cataatcaac aatatagtct ggcaagactt cctgcgacaa tgacaggtaa ccttgtttaa
    36601 aaacttcagt cttcactttt tcaggagcat cataatggaa agcttgatgg ataacctctc
    36661 cgccacgtcc ccagtcttta ccaaaaaggt aaacgtcttt ttcataaagc cccataatag
    36721 taaaggttgc tttgtcacct gtaacggcct tgacttcttt ttcataagct gctgttttag
    36781 ttttccaatc ttttaaccat ttcttggctt tgccttcttt gttaaagatg cggccgaagt
    36841 cagataacac ttgtaaataa tcacgtttgc ggtattcaat cgaaataaca ggcgcaattt
    36901 ctgccaattg tttaatattt tcttctgtag agccaacaac aatgaggtca ggcttaagcg
    36961 ttgtaatggc ttctaaatca gttgcagcaa cttgctttgc tttttttaca gtctttgcta
    37021 agatggggtt tttcttgtca taagaagtaa ccccgactag attcatatct aattttttga
    37081 ggtaaccagt gtaagttgaa gccagactga ctactcgctt aggctgttta ggaatatctc
    37141 cgtaataagt cacaccagca atttgaggca taggcgataa actttttgag gcggtgttag
    37201 aatgattagt tgcttgatta ccacaggcga ttaaggtaat ggttgtcaaa cagagtgtta
    37261 atagtagagt taattttttc atgtatgcta attctcctag aataaagtta ttgaagctga
    37321 taagtcagca ctataggaca gttgtggata ggatcatcaa ccagtaccgg tttgatttgg
    37381 aagatgtctt gaatgatagg tgaagtcata acatctgcta tagtaccagt ataatggatc
    37441 ttgccatgtt tcatagcaat cagatgatcc gaatagcggg ctgaaagatt gaggtcgtga
    37501 agaaccataa caatagtctt gccggcatcc ttatttaggg atttaacgag ctctaggatt
    37561 tctagttgat gatttaaatc taaataagta gttggctcat ctaaaaaaat agtatcagtg
    37621 ccttgggcta atgccattgc aagccaaacc ctttgacgtt gccccccaga aagagcatca
    37681 acaggttgat cagcataggc catgacattg gtggcttgca tagcccaatg aatggctttt
    37741 ttgtcagctg gactaagatt tccaaagtaa gattgatggg gaaaacgtcc gtaagaaacc
    37801 aattcgtaaa cagttatgcc gttagttgct tcttgaactt gaggtaaaag agctagtttt
    37861 ttagcaacct ctttagtttc taatgtggca atgttttgcc catttaaata cacaactccc
    37921 tgttttggtg gtaataatct tgtcagtgct tttaataaac tagacttccc acagccattg
    37981 gcgccaataa tggtcgtaat tttaccttca ggaatataaa acgataattt atcgatgatg
    38041 gtacgctgtt cataggcaat tgtgaggtct tcagcactaa ttgttgtcat atggttgcct
    38101 cctttctaaa aagagtcaaa gggttgatga aagtgcacat taaagtattc atctgcccat
    38161 tttaaaatag ttgagaagta gcttattttc tcaaaatctt cactattata gcatagaaaa
    38221 aaggcttaac aaagcctttt ttgatgtgat ttttaggatg tattaaaaaa gaaatattag
    38281 tttagaatga ttaaaaacaa tagcgagaat cattctaaag taacttcaac atactctaga
    38341 ggtagtgttt ggcgattgct aaatcaccag cataagctac ttgttgtcct ttaacaattt
    38401 gataagcatc tgcgcctttc ccagcgataa tcacagcatc ttttgctcct tggcaaagag
    38461 acatggcttt ttgaatagct tgctcacggt cactaataat ctctaccggg cgtgcgatat
    38521 ggctggcaat ttcttttgaa atatcttctg gatcctcgaa attagggtca tcagcagtga
    38581 gaatgacggt gaggtttgga tgctgatgaa tcaccctacc aaaatcagct cgtctgctct
    38641 cgcctttatt tcctggtgca cctaaaatca acattaattt gcctgtctga tgttcttcca
    38701 caacgctgag taatttttct aagctgtcac cattatgggc ataatcaaca aaaactttag
    38761 catgattagt catggtgagg acttccatac ggcctgggac gcgagtctta gcgatgcctt
    38821 tttgaatatc agctaggcta gcacccaaac gaaggcaggc aagtccagct gccatagcat
    38881 tttcttggtt aaaatggcca attaattgaa tgtcataatg gccagctaat tgtcctttag
    38941 cttcaaagga gaaagcttgg ctagtggtga tctggttgtc agacaaggga ccataaaaca
    39001 catgctcttg atccgctacc tgatcagcta gaaatgaaaa atgatccatc ccgctattga
    39061 tcacaacagc tctgctattc tccattaaaa gacgcttatg atagaaataa tcttcaaacg
    39121 ttggatgctc gattgggcca atatgatcag gactaatatt taggaaaacc ccaacatcga
    39181 acgtaagccc atagacacgg tcaacgaggt aagcctgact agaaacttcc ataatcaagt
    39241 gggtcatgcc attagtcaca cactcagcca tcatagcaaa aagatccaag ctttcaggag
    39301 ttgtaagttg tgatttgaaa aaggtcttgc catctaaggt ggtattcatg gtagaaaaca
    39361 tggcaggttt ataggattct ttcaacatgt ggtaggcaaa ataagcagcc gtcgtctttc
    39421 ccttagtacc tgtaaaagct agtagtttga gcttttcttg agggttgcca tagaaagcca
    39481 tggcaattag actcatagct tttttgatat cagtgaccaa aacaacggga ataccaagct
    39541 cgtaatctac ctctgaaata tagagttgta atccgttagt gattgcttct ttaaggtagt
    39601 cagctttgaa agttgctcct ttagcaaaaa aaagggtctt gccatctact tgacggctat
    39661 cgtaactcaa gcgctcaaaa cttaagcctt gataatggta gtggtatccg tcggcatcaa
    39721 gaacctcacg aaaattatgg tcttttttta aaatgtctaa taattgttca atggttatca
    39781 tacccttatt ttatccttaa agttcttgtt ttacaagtca caagcaaaag tttataataa
    39841 aactaataga aactaggaaa gaatagatat gtcgacagaa aaaaaacaac tgacacaaga
    39901 agaattgatg gttcagggag cagcctggtc cacagctggt aatttcatta gccgactttt
    39961 gggagtgtta tacattattc cctggtatat ttggatgggg caatacgcga ttcaagccaa
    40021 cgctcttttc aatatggggt ataatgttta tgcttatttt ttattgattt caaccacagg
    40081 actcaatgtt gcgattgcta agcaggttgc caagtacaat tccatgggac aaactgagca
    40141 tagctatcaa ttaatccgaa gtaccctaaa actcatgctt ggtctgggcc ttattttttc
    40201 agtcattatg tatctgggct ctcctttatt tgcgagctta tctggtggtg atgacacact
    40261 tgttcccatt atgcacagcc tttctttagc agtttttatt tttcctgtaa tgagtgtgat
    40321 tcgaggtatc tttcaaggcc acaataatat caagccttat gcgattagtc agattgctga
    40381 gcaactcatt cgggttatct ggatgctttt gaccaccttt tttatcatga agttaggttc
    40441 aaaagattat ctatcggcgg taacgcagtc aaccttggca gcctttgtgg gaatgattgc
    40501 tagtatggct gtcttaggtt actacttgtg gaagttaggc ttgctaaaag caatatgtcg
    40561 taagcccaat catcatattg atagtgacat taaaggactg ttgcttgaaa ccttaaaaga
    40621 atccatcccc tttatcgtga caggaagcgc gattcaagcc tttcaattga ttgatcaatg
    40681 gacttttgtc aatactatga ctctttttac agattatagc cgttctcaat tgcttgtttt
    40741 atttggttac tttaacgcaa acccagccaa aattacaatg gttttaattg cagtagcagc
    40801 atctattggt ggtgtaggca ttgccttatt aactgaaaat tatgtcaaaa aagatatgaa
    40861 agcagctgct cgcttaatca ttaacaacat tgaaatgtta gtgatgtttt tgttacccgc
    40921 tcttactggg gcaattattt tagcaagacc tctatattct gttttttacg gagctagcga
    40981 ggatcgtgct attcacctct ttgtggcggt cctcttccaa accttgctac tggcgcttta
    41041 caccctcttt tcaccgatgc ttcaagctct ttttgaaaat cgaaaagcga tttactactt
    41101 tgcctatggt atcttgatta agttagtttt acagataccg cttatttatt tgctacatgc
    41161 ttatggtcct ttactagcga cgacgattgc tttagtggtg ccgatttatt tgatgtatcg
    41221 acgcctatat caggttactc attttaaccg caaactgttg caaaaacgtt tattattaac
    41281 cttgattgaa accttattaa tgggactggt cgtgtttgtg gccaactggc tattgggcta
    41341 tgcctttaaa ccgacaggcc gcttgaccag ccttctttac ctcctcatta ttggtggctt
    41401 gggaatgacg gtttacacgg cactcacctt gctgacgcat caactagata aattaattgg
    41461 tagcaaagct agtcgccttc gtcagaaatt aggctggcat taatataatg acaccaagaa
    41521 cggtttcgtg agtgtttatc gaaatcgttt tttcttaatg atctcgctta gtaaaggtag
    41581 ctgataaaaa atcaggattt cccaagcaaa ctactgatat ttttggtata atgataaaag
    41641 tgtaaaaaaa tagaaggaag tatatcaaca tgggaaaatg tcaagttatt tcgcatccgc
    41701 ttatccaaca caaactttca atcttacgtc gtcaaaccac atcaactaaa gattttcgtg
    41761 agttggttaa tgaaattgcg atgctgatgg gatatgaagt gtcacgcgac cttcccttag
    41821 aagatgtcga catccaaacg ccagtctcaa aaacggtcca aaaacagtta gctggtaaaa
    41881 aattagctat tgttccgatt cttcgtgctg gtattgggat ggttgatggc ttgttaagtt
    41941 tggtaccagc tgcaaaagtt ggccatatcg gcatgtaccg taatgaagaa acccttgaac
    42001 ctgttgaata cctcgttaaa ctaccagaag atattaacca acgacaaatt ttccttgttg
    42061 atccaatgtt ggcaacaggt ggttcggcta tcttagcagt ggattcgttg aaaaaacgtg
    42121 gcgcagcaaa tattaagttt gtctgtttag ttgcagcacc agaaggggtg aaaaaacttc
    42181 aagaagctca tccagatatt gatatcttca cagcagcact tgatgatcat ttaaatgaac
    42241 atggctatat cgtgccaggc ttgggcgatg ctggtgatcg tttatttggg acaaaataaa
    42301 agagacaacc atcaaatagt agccttttta ggccaatcgt tcttgatttt gttaaaaaaa
    42361 tcagagagat ttctcttctt taaaggaaaa gcaagtttat gatttgacct ttattgacca
    42421 aaatattata ataaggacaa tcagtaaaaa agagatgacc tagtgggttg ccgtctctca
    42481 caatgacgct actaaaaacc cttgtagatt ggagaatgta ttatgattcc tgttgttatt
    42541 gaacaaacta gtcgtggaga acgttcttat gacatttatt cacgtttatt aaaagaccgt
    42601 attatcatgt taacagggcc tgttgaagac aacatggcca actctgtcat agcgcagctc
    42661 ttattccttg acgcgcaaga caacacgaaa gatatctacc tatatgtcaa cacaccaggt
    42721 ggctctgtct cagcaggttt ggctattgtc gataccatga actttatcaa ggccgatgtg
    42781 caaactattg tcatgggtat ggcagcttct atggggacag tcattgcttc atcaggaaca
    42841 aaaggcaaac gctttatgtt accaaatgca gagtatatga tccaccagcc aatgggtggc
    42901 acaggcggtg gcacgcaaca aacggatatg gctatcgcag cagaacatct cttaaaaaca
    42961 cgtcaccgtt tagaaaaaat cttagcgcaa aatgctggaa aaacgattaa acaaatccat
    43021 aaagatgctg agcgtgatta ttggatgagt gctgaagaaa ccttggctta cggtttcatt
    43081 gatgaaatca tggaaaacaa cgaactaaaa taaagaggat ctctgcaaaa gtattctaga
    43141 aacttctagg atgctttttc tcttagaaag gaaaataatg atacagccta ctgcattaaa
    43201 acagcttcaa gagttaaaac gtcagttcag atccctttgc aaccaattat gagatagttt
    43261 gaggatgatc cttagcagct gtgtgcaaga agttatgacg atttcataaa ggatgaccta
    43321 gccagtcttt cttcgtctct tttacttaaa gtgtggtata atcagattat gctagccaaa
    43381 actaaaaaat gcattgccat taaaggtgtt gtagatcggc ttatcctaga tctcataaca
    43441 atgctgtagt accgccctta ccttgctact aaaggcgctg ttgaagcaca aattgacttg
    43501 caaaggatct tgactgatag gtatatgtcc ggtggaatcg ccaaagcagc aaaaagacca
    43561 agacccttat ttggtctcta ccaaaatata atagaaaagg atttagaatg tttcaaaaac
    43621 aagaacgtat tggactcgtc gtttacctgt attataaccg agatgccaga aagttatcaa
    43681 aatttggtga tttatattac cattccaagc gctctcgtta cctcattatt tatattaata
    43741 aaaatgattt agacacaaaa ttagaagaaa tgagacgttt gaaatgtgtt aaagacatta
    43801 gaccatcagc ttttgatgat attgaccgcc aatttgtagg taaccttcat cgcgacgaaa
    43861 caaacaacca tcaaaagggt taccaacccc ctagttactg attagcgttt cagatttagt
    43921 tagcttatta aagtaagaag gttttgacag atgctcacac tacttcaaga caatgagaca
    43981 actagagcat tagtgaataa ctgagttgac tgtcacttct ttaataagaa cggataggat
    44041 tggtcctaaa atagagcact gcaaagatat gccagtgctt tttagctcat tttttgataa
    44101 aatagaacta ctaaaaagtg aaggaaataa gatgatcaca ggaaaactca taaccgttga
    44161 aggtccagat ggcgcaggga aaacgactgt tttagagcaa ctcattccac tgttaaaaca
    44221 aaaagtgact caggagatct tgacaacaag agagcctgga ggtgttgcca tttcagaata
    44281 cattagagaa cttattttag acatcaacca tactactatg gaccctaaaa cagaactctt
    44341 actttatatt gcggctcgaa ggcagcattt ggtggaaaag gtactaccag ctcttgaagc
    44401 aggccagttg gtttttattg atcgtttcat cgatagttca gtagcttatc aaggtgcggg
    44461 acgcggtttg atcaaagcag acattcagtg gctcaacgag ttcgcaacag atggtttaga
    44521 gcctgacttg accctttatt ttgatgttcc ttctgaaatt ggtttagcac ggattaacgc
    44581 gaaccaacaa agagaagtca atcgtttaga tttggaaaca atagagatac atcaacgagt
    44641 acgaaaagga tacctagcct tagctaagga gcatccaaaa cgtattgtca caattgatgc
    44701 gactaaacca ttgaaagaag ttgtctctgt cgctttggaa catgtcttag ccttgctttt
    44761 agcctaggat aagggagggt aggaatggat ttagcgcaaa aagctcctaa cgtttatcaa
    44821 gcttttcaga cgattttaaa gaaagaccgt ctgaatcatg cttatctttt ttcgggtgat
    44881 tttgctaatg aagaaatggc tcttttttta gctaaggtca tcttttgtga acagaaaaag
    44941 gatcagacgc cctgcgggca ttgtcgctct tgtcaattga ttgaacaagg agattttgcc
    45001 gatgtgacgg tattggaacc aacagggcaa gtgattaaaa cggatgtggt caaagaaatg
    45061 atggctaact tttctcagac aggatatgaa ggaaaacgac aagtttttat tatcaaagat
    45121 tgtgaaaaaa tgcatgtaaa tgccgctaat agcttgctaa aatacattga ggagcctcag
    45181 ggagaagctt acatgttttt gctgaccaat gatgataaca aagtgcttcc gaccattaaa
    45241 agtcggacac aggtttttca gtttcctaaa aacgaagcct atctcttcca gttagcccaa
    45301 gagcgaggtt tattaagaag tcaagctgat ttagtggcca aactggctaa aaatgttgac
    45361 caattagaag atttggcaca aaatagtcat tttttagaag tgatggctca gactgaacgt
    45421 tttgtttcaa tctggttaaa agatcagctg caagcttatt tagccttgaa tcacctggta
    45481 ctgttggcaa ccgaaaaaga ggagcaagag ttaattttga cccttttgac actactcttg
    45541 gcaagagagc aatcacaaac gccctttaaa caattggagg cggtttatca ggctaggctc
    45601 atgtggcaga gcaatgtcaa ttttcaaaac acattagaat atatggtgat gtcagaataa
    45661 gtgcaattgg aattcgttat ggggaaacga agcttgtcaa aagacttgcc cgtagcgacc
    45721 aaagtagcgg cgcatagggg caaaaaagct tttgaagaaa atactgccct tgcaacagat
    45781 ttgatgaaag aagtatcacg attaatgata caaaatcaac ttgaaatgac gtttattaat
    45841 agtgtctttc ccttagatca gcatgttgtc ctaattccct tttcagcaga agagcacatg
    45901 gatttttaca actgttaaaa gatttagcgg ccgattttag gtcaagaagt gagctgcccc
    45961 agattaacag ccaagaagaa accaagattt atgggcgctt aggaccgtgt ggcagaccac
    46021 tgtgctgctt tcgtttttgg gaaaatttct gacagtatct attaaaatac taaaaaatca
    46081 aaggttatca ttgactttgg gtaaagcaac aagcccttat ggccgtttgc tttgctgtct
    46141 ctatgatgaa ggtggctttt accaataaag caagaaaaaa ttccctgaat atggtaccat
    46201 agtagagaca agtgatggcc ctgcaacagt agcagccgtt gatatattta cagatcccat
    46261 taaggtgcgg ttaaaagacc agttgacctt agtcacttat gctttagagg aggttaaagt
    46321 aggtgaataa aaaagaatta ttcgatgctt ttgatggatt ttcccaaaat ttaatggtta
    46381 ctttagctga aattgaagcc atgaaaaaac aagtccaatc actagtagaa gaaaacacga
    46441 ttcttcgcct agaaaataca aaactacggg aacgtttgag tcacctagaa catgaaacgg
    46501 tagctaaaaa tccttccaaa caaaggaagg atcacttaga gggaatttat gatgaagggt
    46561 ttcatatctg caattttttc tatggacaac gtcgtgaaaa tgatgaagaa tgtatgtttt
    46621 gcagagaatt attagataga aagtaagggc atgcaggttc aaaaaagttt taaagacaaa
    46681 aaaacaagcg gaaccttgta tttggttcca accccaattg gcaatctgca ggacatgacc
    46741 tttagagcag tggcaacgtt aaaagaggtt gattttattt gtgcagaaga cacaagaaac
    46801 acaggtcttt tgctaaaaca ctttgacatt gctaccaagc aaatcagctt tcatgagcac
    46861 aacgcctatg agaagattcc tgatttaatt gatttattaa tatcaggacg gtcactagct
    46921 caggtatctg atgcaggaat gccatcgatt tctgatcctg ggcatgattt ggtcaaggct
    46981 gctattgata gtgatatcgc agtcgttgct ttaccaggtg cctcagcagg gattacagct
    47041 ttgattgcta gtggactggc tcctcaaccc catgtttttt atgggttttt acccagaaaa
    47101 gcagggcaac aaaaggcttt ctttgaggac aaacaccatt acacagaaac acaaatgttt
    47161 tatgaatcac cctatcgcat caaagatacc ctgaccaata tgttggcttg ttatggtgat
    47221 cgtcaagtgg tgcttgttag agaattaacc aagctttttg aggagtacca acgggggtcg
    47281 atttcagaaa tcttgtccta ccttgaggaa acacccctaa aaggggagtg tctgctgatt
    47341 gtggctggag cccaagtaga cagtgagatt gagctgacag ctgatgttga cttagtgtct
    47401 ctagtgcaaa aagaaatcca agcaggagcc aagccaaatc aggctattaa aacaattgct
    47461 aaagcttacc aggtaaatcg ccaagagctt taccaacaat ttcatgactt ataagtcatc
    47521 gtagattagg agaaaaacaa atgacagaag atcaagcaac tatccttatt ttaggagtat
    47581 acggaatcat attggctgta ttcttattat cagctttatt atgccttatc gctaactggc
    47641 gactctttaa aagagcaggt tacaaaggat ggtatagtct cattcctttc tatgcaggtt
    47701 ttgtcaaaca caaaatcact tttggagaag ataataaatg gttttacttt attggttggg
    47761 tcattgctat ttattattac tatgtgcact tttgctatgt ccgtgctttt ggtggatcaa
    47821 agggtctagc agtatttggc ttgtttttcc caggcattgc ttctttgata gtggcttttg
    47881 gaaaaactta taccacacaa gaatgccacc ctgtgccaca tcttttaaac taagagaaaa
    47941 aagaccatca attggattta ttgatggtct tttaggtgtt taaatgattt tagtcccgtg
    48001 aacaatggcc gttccagtga gttgactcaa gttttggcaa ttctcagcag tgacgcctcc
    48061 tccaatcatg atttcaatgc gtttattggc ataggtcaca agacttttaa gctgttccac
    48121 attatctgta ataggagtag cttcagggga gccatgagtt aacacacgca caaagccata
    48181 atcaatcagt tgatcaagcg cttggtgctg gtggtcagtc ggaatatggt caaaagccat
    48241 gtggaaaacc agtggcaagc cttgtgtagc aggcagtaac tgctcgatag cgtcggtatc
    48301 aaggtgattt tctgtggtca gcaagcctaa gactagcgca tctgagccag cttcaacagc
    48361 ttttaagata tcttcttcca tagcttttag ttcgaggtcg ttatagacaa aatcgccacc
    48421 gcgaggtcga atcatggtcg ctacgctaat ctttttatcg tgtaagagct gacaggcttc
    48481 tttgatgacg ccataagatg gtgttgttcc cccgacagct aggttatcac agagttccac
    48541 acgactgact tgaccagcat caagggttgg cagtagtgtg aggttctcag cacaaaattc
    48601 tttaatcatt tttagatctc ctttatcact aatattatag cacgaatgaa gctagtcatc
    48661 aggaaacatt tttgtcaaat catggacatt atgttatact cactagtatt aaaagtaggc
    48721 caaaacagca gcaaatactt tgaaaaaaga ttgctatgtt ctacggcaat atcagtcttt
    48781 accaacgaac tctcaagaag aaaagaaaga caaagcttag cattttaaaa aagcccccct
    48841 ttttttgtga aatgatgacc aaaaacaggc gcctcacgcc taaaacagtt accctaaagg
    48901 agaacaaatg attacgattt accaataccc aaaatgtagt acctgtcaaa gagccatggc
    48961 tgaactaaag caactggtta gtgactttga agtcattgac atcaaagcta atccacccaa
    49021 agctcaggac ctcaaacatt ggatggaaac atctggttat accatcaaaa atttctttaa
    49081 taccagtggt aacagctacc gcgaattggg acttaaagat aaaatagacc agcttagcct
    49141 tgataaagct gctgaactct tagccacaga tggtatgctg attaaacggc ctattctgat
    49201 taaggacggt agagtcctac aagttggcta tcgcaagcca tatcaagagt tggatttagg
    49261 ataatgcgac aaagtctcaa aaaaggacaa ggcaacatta taacgccttg tccttttttg
    49321 ggatctgttt ttagaaatta gagatcaata tctagtaaaa ttggcgtatg gtcttggcgt
    49381 tccccagaag aaatcatttc agaacgctta accttatcca caagtcggtt tgaagctagc
    49441 caatagtcaa ttctccaacc ggtattattg attttgctgg ttttgctacg ttgtgcccac
    49501 caggtataaa catgaggaat atcgccgtgc aagtgacgaa aagtatctgt aaagcctctt
    49561 gctaataggt tagtaaagcc ttgacgttct tcgtcggtaa aaccagggga acgtcgattg
    49621 ctgcttggat ttgccaaatc aatctctttg tgggcaacgt tgtaatctcc cgtagccaaa
    49681 acaggctttt gagcatccag ttcagtcaag taatcagcgt atttgtggtc ccaaatttga
    49741 cggtcatcta aacgtctaag gccatcacca gcattaggcg tgtaaacctg tgtgacgaaa
    49801 aagtcttcaa attctagggt aatgatacgg ccctcagcat ccattgttgt tggggcacca
    49861 atttctggaa aagtaatgac agggtttagc gtgtttttgt acaggaacat ggtgccagca
    49921 tagcctttcc tagcaggctc aactgaagaa cgccacacat gcaaatagtt tggaaagtaa
    49981 ctaagtaagg cctcgatatg ttttttggta ggccccttag cagataattt ggtttcttga
    50041 atggcaataa tatccgcatc ttgagcgact aaagtgtcta aaacagcgcg tgataaaagc
    50101 gcacgagggg actctcctgt gagagcagca tttaaggaat caatgttcca tgaaatcagt
    50161 ttcatttggt atctctttct aacagatagt cttgttgtta ttgtaacaaa aaacgaaacg
    50221 ataagcgact ttggacacct agcccccata aaacagtcta gccaaatgaa gcatagcagc
    50281 gtaagaacaa gataaggagg tgatcagtga gcatatcgtt tgacagttaa gagtaggatt
    50341 actataatag aagcataaat gtaagcgcta acaaaataaa aaatatagga gaatgtcaat
    50401 ggcacaaaaa acagtcatca ccgaggaaac aaccgatttt gttatggatt tcaaaacaag
    50461 tagcgctgag ggaaatgttg attttattaa cgtttttgac cttgagaaaa tggcacaaca
    50521 ggtcatccct aaaggagcct ttggttatat tgctagtggt gctggtgata cctttaccct
    50581 ccatgaaaat attcgctcat ttaatcataa attaattgtc cctcactccc tcaaaggggt
    50641 tgaaaatccg tcaacagaga ttacgtttga tggtgatcac ttaacctctc ctcttatttt
    50701 agcacctgta gcggctcata aactggccaa tgaacaagga gaagtagcgt ctgccaaagg
    50761 attgaaggaa tttggaagta tctacaccac tagttcctac tcaacaactg acctgccaga
    50821 aatcagtgct gccttaggtg ggacacccca ttggtttcaa ttttattaca gtaaagacga
    50881 tggcattaat cgtaatatta tggatcgtgt caaagcacaa ggttgcaaag ctatcgtctt
    50941 gacagcagat gcaacagttg gaggaaaccg cgaagttgat aggcgaaacg gttttgtttt
    51001 tccggtcggt atgccaattg ttcaagaata tttacctgat ggtgcaggta aaacaatgga
    51061 ttatgtctat aaatcagcta aacaagcgct tacctcaaaa gatattgaat atatcgcaac
    51121 ctactcagga ctgcctgttt atgtcaaagg accacagtgt gctgaggata ccttgcgggc
    51181 attggacgca ggagcatcag gtatctgggt aaccaatcat ggtggccgtc aattagatgg
    51241 tggtccagct gcttttgatt ccttacaaga agtcgctgaa gcagttgatc aaaaagttcc
    51301 tattgtcttt gactcaggta taagacgggg tcaacatatt ttcaaagctc ttgcttcagg
    51361 tgctgacctt gttgccttgg ggcgtccagc aatttacggc ttggctatgg gaggaagcac
    51421 tggtactcgt caggtctttg aaaagttaaa tgacgagtta aaaatggtta tgcaactggc
    51481 tgggacacaa actatccaag atgttaaagc ctttaacctt cgccataatc cgtatgattc
    51541 atcgattcca tttgatcaaa atgcgctacg cttagattaa aacagacaaa aacctctgct
    51601 atcatgtcac aaatagtaga ggttttacac tgcaaaaaga ggtatgaaaa tccaataaca
    51661 tagttacaag gctatcgttt accaccccac taatagatta aaatgaaaga cttcttacat
    51721 taatagaagt ttctgttgct tttctcagaa aaatgtggtt aaataatcaa atattattaa
    51781 cgaaaaatga gttaatgatt aacattaaac tggttttagt taaaacattt aggagggtat
    51841 caggtggaga aaaagcaacg tttttccctt agaaaataca aatcaggaac gttttcggtc
    51901 ttaataggaa gcgttttctt gatgatgaca acaacagtag cagcagatga gctaaccaca
    51961 acgagtgaac caacaatcac gaatcacgct caacaacaag cgcaacatct caccaataca
    52021 gagttgagct cagctgaatc aaaacctcaa gacacatcac aaatcactcc caagacaaat
    52081 cgtgaaaaag agcaatcaca agatctagtc tctgagccaa ccacaactga gctagctgac
    52141 acagattcag caccaatggc taatacaggt tctgatgcga ctcaaaaaag cgcttcttta
    52201 ccgccagtca atacagatgt tcacgattgg gtaaaaacca aaggagcttg ggacaaggga
    52261 tacaaaggac aaggcaaggt tgtcgcagtt attgacacag ggatcgatcc ggcccatcaa
    52321 agcatgcgca tcagtgatgt atcaactgct aaagtaaaat caaaagaaga catgctagca
    52381 cgccaaaaag ccgccggtat taattatggg agttggataa atgataaagt tgtttttgca
    52441 cataattatg tggaaaatag cgataatatc aaagaaaatc aattcgagga ttttgatgag
    52501 gactgggaaa actttgagtt tgatgcagag gcagagccaa aagccatcaa aaaacacaag
    52561 atctatcgtc cccaatcaac ccaggcaccg aaagaaactg ttatcaaaac agaagaaaca
    52621 gatggttcac atgatattga ctggacacaa acagacgatg acaccaaata cgagtcacac
    52681 ggtatgcatg tgacaggtat tgtagccggt aatagcaaag aagccgctgc tactggagaa
    52741 cgctttttag gaattgcacc agaggcccaa gtcatgttca tgcgtgtttt tgccaacgac
    52801 gtcatgggat cagctgaatc actctttatc aaagctatcg aagatgccgt ggccttagga
    52861 gcagatgtga tcaacctgag tcttggaacc gctaatgggg cacagcttag tggcagcaag
    52921 cctctaatgg aagcaattga aaaagctaaa aaagccggtg tatcagttgt tgtagcagca
    52981 ggaaatgagc gcgtctatgg atctgaccat gatgatccat tggcaacaaa tccagactat
    53041 ggtttggtcg gttctccctc aacaggtcga acaccaacat cagtggcagc tataaacagt
    53101 aagtgggtga tccaacgtct aatgacggtc aaagaattgg aaaaccgtgc cgatttaaac
    53161 catggtaaag ccatctattc agagtctgtc gactttaaaa acataaaaga tagcctaggt
    53221 tatgataaat cgcatcaatt tgcttatgtc aaagagtcaa ctgatgcggg ttataacgca
    53281 caagacgtta aaggtaaaat tgctttaatt gaacgtgatc ccaataaaac ctatgacgaa
    53341 atgattgctt tggctaagaa acatggagcc ctgggagtac ttatttttaa taacaagcct
    53401 ggtcaatcaa accgctcaat gcgcctaaca tctaatggga tggggatacc atctgctttc
    53461 atatcgcacg aatttggtaa ggccatgtcc caattaaata ccaatggtac aggaagttta
    53521 gtgtttgaca gtgtggtctc aaaagcaccg agtcaaaaag gcaatgaaat gaatcatttt
    53581 tcaaattggg gcctaacctc tgatggctat ttaaaacctg acattactgc accaggtggc
    53641 gatatctact ctacctataa cgataaccac tatggtagcc aaacaggaac aagtatggcc
    53701 tctcctcaga ttgctggcgc cagccttttg gtcaaacaat acctggaaaa gactcagcca
    53761 aacttgccaa aagaaaaaat tgctgatatc gttaagaacc tattgatgag caatgctcaa
    53821 attcatgtta atccagaaac aaaaacgacc acctcaccgc gtcagcaagg ggcaggatta
    53881 cttaatattg acggagctgt cactagcggc ctttatgtga caggaaaaga caactatggc
    53941 agtatatcat taggcaacat cacagatacg atgacgtttg atgtgactgt tcacaaccta
    54001 agcaataaag ccaaaacatt acgttatgac acagaattgc taacagatca tgtagaccca
    54061 caaaagggcc gcttcacttt gacttctcgc tccttaaaaa cgtaccaagg aggagaagtt
    54121 acagtcccag ccaatggaaa agtgactgta acggttacca tggatgtctc acagttcaca
    54181 aaagagctaa caaaacagat gccaaatggt tactatctag aaggttttgt ccgctttaga
    54241 gatagtcaag atgaccaact aaatagagta aacattcctt ttgttggttt taaaggacaa
    54301 tttgaaaact tagcagttgc agaagagtcc atttacaggt taaaatctca aggcaaaacc
    54361 ggtttttact ttgatgaatc aggtccaaaa gacgatatct atgtcggtaa acactttaca
    54421 ggactcgtca ctcttgggtc agagaccaat gtgtcaacca aaacgatttc tgacaacggt
    54481 ctacacacac ttggtacctt taaaaatgca gatggcaaat ttatcttaga aaaaaatgcc
    54541 caaggaaacc ctgtcttagc catttctcca aatggtgata acaaccaaga ttttgcagcc
    54601 ttcaaaggtg ttttcttgag aaaatatcaa ggcttaaaag caagtgtcta ccatgctagt
    54661 gacaaggaac acaaaaatcc actatgggtc agcccagaaa gctttaaagg agataaaaac
    54721 tttaatagtg acattagatt tgcaaaatcg acgaccctgt taggcacagc gttttctgga
    54781 aaatcgttaa caggagctga attaccagat gggtattatc attatgtggt gtcttattac
    54841 ccagatgtgg ttggtgccaa acgtcaagaa atgacatttg acatgatttt agaccgacaa
    54901 aaaccggtac taccacaagc aacatttgat cctgaaacaa accgattcaa accagaaccc
    54961 ctaaaagacc ggggattagc tggtgttcgc aaagacagtg tcttttatct agaaagaaaa
    55021 gacaacaagc cttatacagt tacgataaac gatagctaca aatatgtctc agtagaagac
    55081 aataaaacat ttgtggagcg acaagctgat ggcagcttta tcttgccgct tgataaagca
    55141 aaattagggg atttctatta catggtcgag gattttgcag ggaacgtggc catcgctaag
    55201 ttaggagatc acctaccaca aacattaggt aaaacaccaa ttaaacttaa gcttacagac
    55261 ggtaattatc agaccaaaga aacgcttaaa gataatcttg aaatgacaca gtctgacaca
    55321 ggtctagtca caaatcaagc ccagctagca gtggtgcacc gcaatcagcc gcaaagccag
    55381 ctaacaaaga tgaatcagga tttctttatc tcaccaaacg aagatgggaa taaagacttc
    55441 gtggccttta aaggcttgaa aaataacgta tataatgact taacggttaa cgtatacgct
    55501 aaagatgacc accaaaaaca aacccctatc tggtctagtc aagcaggcgc tagtgcatca
    55561 gctattgaaa gtacagcctg gtatggcata acagcccgag gaagcaaggt gatgccaggt
    55621 gattatcagt atgttgtgac ttatcgtgac gaacatggta aagaacatca aaagcagtac
    55681 accatatctg tgaatgacaa aaaaccaatg atcactcagg gacgttttga taccattaat
    55741 ggcgttgacc actttactcc tgacaagaca aaagcccttg gctcatcagg cattgtccgc
    55801 gaagaagtct tttacttggc caagaaaaat ggccgtaaat ttgatgtgac agaaggtaaa
    55861 gatggtatca cagttagtga caataagatg tatatcccta aaaatccaga tggttcttac
    55921 accatttcaa aaagagatgg tgtcacactg tcagattatt actaccttgt cgaagataga
    55981 gctggtaatg tgtcttttgc taccttgcgt gacctaaaag cggtcggaaa agacaaagca
    56041 gtagttaact ttggattaga cttaccggtc cctgaagaca aacaaatagt gaactttact
    56101 taccttgtgc gggatgcaga tggtaaaccg attgaaaacc tagagtatta taataactca
    56161 ggtaacagtc ttatcttgcc atacggcaaa tacacggtcg aattgttgac ctatgacacc
    56221 aatgcagcca aactagagtc agataaaatc gtttccttta ccttatcagc tgataataac
    56281 ttccaacaag ttacctttaa gatgacgatg ttagcaacct ctcaaataac tgcccacttt
    56341 gatcatcttt tgccagaagg cagtcgcgtt agccttaaaa cagctcaagg tcagctgatc
    56401 ccgcttgaac agtccttgta tgtgcctaaa gcttatggca aaaccgttca agaaggcact
    56461 tacgaagttg ttgtcagcct gcctaaaggc taccgtatcg aaggcaacac aaaggtgaat
    56521 accctaccaa atgaagtgca cgaactatca ttacgccttg tcaaagtagg agatgcctca
    56581 gattcaactg gcgatcataa ggttatgtca aaaaataatt cacaggcttt gacagccttt
    56641 gccacaccaa ccaagacaac gacctcagca acagcaaaag ccctaccatc agcgggtgaa
    56701 aaaatgggtc tcaagttgcg catagtaggt cttgtgttac tcggacttac ttgcgtcttt
    56761 agccgaaaaa aatcaaccaa agattgataa caaaacgcaa agagacaaca aatcatccct
    56821 tttaacagtc ggacctttct ttgaaattga gctggcagat gatcatttct taacaaaagt
    56881 taaagagttg agcaaagtaa ggaacagaca taaagaccat aaaagcaagc cagtttggct
    56941 tgcttttatg gtgttattgt cgtgtgctac gatgactaaa aaagaacaac gaacaatgac
    57001 ctcgaatcac caaaaggact tgatttgata gcttccctat gctatactaa gcctattctt
    57061 tcaagaagga ctccttttgt ggaaaagact gaaaaaaagg acactaatct atccaaaaac
    57121 tccagatgag tagctgcaac cggatgcctc tgctgtgatc aaggtgataa agcagactgg
    57181 cttgtcacta cctgagcagg tgtcaaaaaa agaagctttg gctttgtcat tcaacatact
    57241 gagcttcagt ctttcagaac ggagacaccc ccagttttgt actcaaaaag cataaacagc
    57301 tctgctgatg cgacatccga tttctacgtc cgcagtgagc caaaccagct gctgtttgct
    57361 atggaaaaag tggcgccaat cgggattttt gagaccctag gctcaaaaat aaggcataga
    57421 atcccaaagg aagtcgctgc cgtgagcaca acctaagaaa aactatccca aaagactttt
    57481 tctgcaattt gaaatactga atttcagtat tttttctttt gtaactaaga gcaatgaatg
    57541 aggtaaaaaa ggcaacaatg tcaaaaaaga aacgaaaaca ctatcaggtt tatgagggat
    57601 tacgctgtgc tatgacgctt tgttttatca gtggctatgt caatgccttt acctatatga
    57661 cacaaggcaa acgttttgca ggtgttcaga caggcaatct tttgtcattt gccattcgct
    57721 tgtcagaaca gcaactgaag gaggcactgc aatttttact tcccatgata gtttttatgc
    57781 ttgggcagtc ctttacttat ttcatgcacc gctgggccac taaaaaggga ctccactggt
    57841 atctcttatc aagcgttatg ttaacaggac ttgcttttgg aacggcccta ttcacgcctt
    57901 ttcttccttc caatgttacg gtagcagctt tagccttttt tgcctctatt caggttgata
    57961 cgtttaaaac cttacgagga gctagctatg ctaatgtgat gatgacagga aatatcaaaa
    58021 atgccgccta tttactaacc aaaggcttgt acgaaaaaaa tcacgaacta acacatatcg
    58081 gacgcaacac cttaatagtc atcttggctt ttgctgttgg agtggtttgt tcgacgttgc
    58141 tttgcatagc ttatggcgaa tatgctttga tgcctatttt aatgccttta ctctatgtca
    58201 attacctgtt ggctcaggaa ttttatcaca ttcaaaccaa aatcaaaccg attaaaagaa
    58261 cttaacggaa ttgtggagaa agaaagttca tttagggtaa gtttttactg cccctcctt
//
LOCUS       DAUCWO010000005        49234 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUCWO010000005 DAUCWO010000000
VERSION     DAUCWO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49234
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.005"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            102..1094
                     /locus_tag="VGY20_000272"
     CDS             102..1094
                     /locus_tag="VGY20_000272"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ4273160.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            1217..1756
                     /gene="rsmD"
                     /locus_tag="VGY20_000273"
     CDS             1217..1756
                     /gene="rsmD"
                     /locus_tag="VGY20_000273"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ4273161.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            1746..2237
                     /gene="coaD"
                     /locus_tag="VGY20_000274"
     CDS             1746..2237
                     /gene="coaD"
                     /locus_tag="VGY20_000274"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ4273162.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            2224..3261
                     /locus_tag="VGY20_000275"
     CDS             2224..3261
                     /locus_tag="VGY20_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ4273163.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPIT
                     TVQEALVYLRK"
     gene            3675..4652
                     /locus_tag="VGY20_000276"
     CDS             3675..4652
                     /locus_tag="VGY20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4273164.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            5076..5720
                     /locus_tag="VGY20_000277"
     CDS             5076..5720
                     /locus_tag="VGY20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ4273165.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPAIPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            5724..6803
                     /gene="rlmN"
                     /locus_tag="VGY20_000278"
     CDS             5724..6803
                     /gene="rlmN"
                     /locus_tag="VGY20_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ4273166.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(6885..7511)
                     /locus_tag="VGY20_000279"
     CDS             complement(6885..7511)
                     /locus_tag="VGY20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEQ4273167.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            7739..8266
                     /locus_tag="VGY20_000280"
     CDS             7739..8266
                     /locus_tag="VGY20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ4273168.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            8408..8611
                     /locus_tag="VGY20_000281"
     CDS             8408..8611
                     /locus_tag="VGY20_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ4273169.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            8616..9587
                     /locus_tag="VGY20_000282"
     CDS             8616..9587
                     /locus_tag="VGY20_000282"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ4273170.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFAYSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            9597..9986
                     /locus_tag="VGY20_000283"
     CDS             9597..9986
                     /locus_tag="VGY20_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ4273171.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRTKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMHPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            10169..12010
                     /gene="typA"
                     /locus_tag="VGY20_000284"
     CDS             10169..12010
                     /gene="typA"
                     /locus_tag="VGY20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ4273172.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            12132..12383
                     /locus_tag="VGY20_000285"
     CDS             12132..12383
                     /locus_tag="VGY20_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ4273173.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            12739..14097
                     /locus_tag="VGY20_000286"
     CDS             12739..14097
                     /locus_tag="VGY20_000286"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ4273174.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     RHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            14097..15179
                     /locus_tag="VGY20_000287"
     CDS             14097..15179
                     /locus_tag="VGY20_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ4273175.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISFAIKEK"
     gene            15180..16328
                     /locus_tag="VGY20_000288"
     CDS             15180..16328
                     /locus_tag="VGY20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ4273176.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTTKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMHDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQTPPSPETVLEQAHG"
     gene            16540..17904
                     /gene="ftsA"
                     /locus_tag="VGY20_000289"
     CDS             16540..17904
                     /gene="ftsA"
                     /locus_tag="VGY20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ4273177.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            17928..19247
                     /gene="ftsZ"
                     /locus_tag="VGY20_000290"
     CDS             17928..19247
                     /gene="ftsZ"
                     /locus_tag="VGY20_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ4273178.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTSAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            19250..19921
                     /locus_tag="VGY20_000291"
     CDS             19250..19921
                     /locus_tag="VGY20_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ4273179.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKGSIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            19933..20589
                     /locus_tag="VGY20_000292"
     CDS             19933..20589
                     /locus_tag="VGY20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ4273180.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMN
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            20594..20848
                     /locus_tag="VGY20_000293"
     CDS             20594..20848
                     /locus_tag="VGY20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ4273181.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            20848..21639
                     /locus_tag="VGY20_000294"
     CDS             20848..21639
                     /locus_tag="VGY20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ4273182.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            21649..22407
                     /locus_tag="VGY20_000295"
     CDS             21649..22407
                     /locus_tag="VGY20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ4273183.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            22680..25481
                     /locus_tag="VGY20_000296"
     CDS             22680..25481
                     /locus_tag="VGY20_000296"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ4273184.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRNDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLNSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGVAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(25746..26048)
                     /locus_tag="VGY20_000297"
     CDS             complement(25746..26048)
                     /locus_tag="VGY20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ4273185.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(26099..26554)
                     /locus_tag="VGY20_000298"
     CDS             complement(26099..26554)
                     /locus_tag="VGY20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4273186.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(26682..28964)
                     /locus_tag="VGY20_000299"
     CDS             complement(26682..28964)
                     /locus_tag="VGY20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4273187.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTSNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            29262..29492
                     /locus_tag="VGY20_000300"
     CDS             29262..29492
                     /locus_tag="VGY20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ4273188.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            29619..30305
                     /locus_tag="VGY20_000301"
     CDS             29619..30305
                     /locus_tag="VGY20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4273189.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMSQGGNH"
     gene            30305..31039
                     /locus_tag="VGY20_000302"
     CDS             30305..31039
                     /locus_tag="VGY20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273190.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            31188..32597
                     /locus_tag="VGY20_000303"
     CDS             31188..32597
                     /locus_tag="VGY20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ4273191.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            32775..34469
                     /locus_tag="VGY20_000304"
     CDS             32775..34469
                     /locus_tag="VGY20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ4273192.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            34676..35530
                     /locus_tag="VGY20_000305"
     CDS             34676..35530
                     /locus_tag="VGY20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ4273193.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVALDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            35683..37023
                     /locus_tag="VGY20_000306"
     CDS             35683..37023
                     /locus_tag="VGY20_000306"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ4273194.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQLLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            37001..37216
                     /locus_tag="VGY20_000307"
     CDS             37001..37216
                     /locus_tag="VGY20_000307"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ4273195.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            37216..38088
                     /locus_tag="VGY20_000308"
     CDS             37216..38088
                     /locus_tag="VGY20_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ4273196.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLENDVGFKP
                     QIITKLIEGLRLNA"
     gene            38081..38908
                     /locus_tag="VGY20_000309"
     CDS             38081..38908
                     /locus_tag="VGY20_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ4273197.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFSEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            38895..39365
                     /locus_tag="VGY20_000310"
     CDS             38895..39365
                     /locus_tag="VGY20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4273198.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            39387..41048
                     /gene="recN"
                     /locus_tag="VGY20_000311"
     CDS             39387..41048
                     /gene="recN"
                     /locus_tag="VGY20_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ4273199.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLEAELKSLEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            41220..42149
                     /locus_tag="VGY20_000312"
     CDS             41220..42149
                     /locus_tag="VGY20_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4273200.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLSD
                     TALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYEDR
                     KAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKIA
                     LPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            42365..43216
                     /locus_tag="VGY20_000313"
     CDS             42365..43216
                     /locus_tag="VGY20_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4273201.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILTAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            43209..44051
                     /locus_tag="VGY20_000314"
     CDS             43209..44051
                     /locus_tag="VGY20_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ4273202.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            44029..44616
                     /locus_tag="VGY20_000315"
     CDS             44029..44616
                     /locus_tag="VGY20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ4273203.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            44715..44990
                     /locus_tag="VGY20_000316"
     CDS             44715..44990
                     /locus_tag="VGY20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ4273204.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(45079..46221)
                     /locus_tag="VGY20_000317"
     CDS             complement(45079..46221)
                     /locus_tag="VGY20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ4273205.1"
                     /translation="MWIEETDNGKFKFRENYKDPYTGTWKPVSVTMEKDNSRAYKAAR
                     KILEQKITEKIAQLKASELLFTELLDEWWAFYKKELKRSSVASLRGNIEEIRETFGIG
                     VKVVNIDPKYVQNYLDNLDCSRNKKERNKSMLNLAFDYAVGLDIIQDNPARRAKLPRV
                     KKTLEDWKKAEEKYLEEDEIKPLLKELYRRPSTYRLGLLAEFISLNGCRIGEAVSIEP
                     CNYESKSRILQLHGTFDHTEGYRNGEKTAPKTLASYRETIMTSRELEILQELEFMNEL
                     EKNTNHRYRDMGYLFTTKNGVPIQTNSFNLALKKANERLEDPITKKLTSHIFRHTLIS
                     RLAENNVPLKSIMERVGHADAKTTAQIYTHVTKKMKSSVADIMENY"
     gene            complement(46345..46863)
                     /locus_tag="VGY20_000318"
     CDS             complement(46345..46863)
                     /locus_tag="VGY20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEQ4273206.1"
                     /translation="MAKVIFQDNFLLMGTNYHEKEANKVMAEIGKKSPYWDKDKDFIS
                     DYIKSNFKDIYKYYRVSTKDVEIVREPLNRHDPNAIKVMVNKTFVGYFPADLAKRLTP
                     YVKKSSHYQMEATLTGRGGQYKTLKNDLKTVVTKKKDITYKLRLTILKVDRVSKSKNA
                     GLLESIASWFLN"
     gene            complement(46875..47630)
                     /locus_tag="VGY20_000319"
     CDS             complement(46875..47630)
                     /locus_tag="VGY20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273207.1"
                     /translation="MEQLGDRIRKLREGRNMTQTELSEILGMKTYTTVSKWEKNENFP
                     KGKDLKKLAEIFNVTSDYLLGLTDSKLGKITIQNEQPEIVSIYNQLEQPRQEKVLNFA
                     NEQLEEQNKTVSIFDKKSEETEDYITDYVEGLVAAGLGAYQEDNLHMKVKLRSDDVPD
                     EYDTIAKVAGDSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGKNFVKKLKRDYDGAW
                     YLQSLNKSYEEIYLSKDDDIRTIGEVVDIYREY"
     gene            47832..48044
                     /locus_tag="VGY20_000320"
     CDS             47832..48044
                     /locus_tag="VGY20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273208.1"
                     /translation="MVTIAELRAKHNKMSQRELAKELGVTQTSISNWEKDQTKISGEY
                     LISLALFFDVSTDDILGINKQATQKF"
     gene            48314..48625
                     /locus_tag="VGY20_000321"
     CDS             48314..48625
                     /locus_tag="VGY20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ4273209.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYKGFKLFVQWKSRNRYKTKKETLPEMLENIKFEKRVG
                     V"
     gene            48627..48812
                     /locus_tag="VGY20_000322"
     CDS             48627..48812
                     /locus_tag="VGY20_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273210.1"
                     /translation="MNKLELFLLATTVILAVIARVQHEVIKKHNSPENKRKIFREVAL
                     KNSKGWSKKRARGGVVS"
BASE COUNT        15715 a         8389 c        10209 g        14921 t
ORIGIN      
        1 caaaatatta gaataaacaa aagtagacaa cgtataaaga gaactgctaa gtcgttgagt
       61 tcattagtcc caaaaagatt accaaaaaag aaggaagaac tatgaagaaa agttttattc
      121 atcaacaaga ggagatttcc tttgttaaaa atacgttcac tcagtattta attgctaagt
      181 tagatgttgt tgaagtgcaa ggacccattt taagtcgggt cggggatggt atgcaagata
      241 atttgtccgg tactgaaaat ccagtatctg tcaatgtctt gaaaattcct aatgcaacct
      301 ttgaagttgt gcattcatta gcaaaatgga aacgtcacac cttagcccgt tttgggttta
      361 atgaaggtga aggccttgtt gttaatatga aggctctccg acctgatgag gattcacttg
      421 accaaaccca ttctgtctat gttgaccaat gggactggga aaaagtcatc cctgatggca
      481 aacgtaatct cgcttacctc aaagaaaccg ttgaaaccat ttacaaggtc attcggctaa
      541 ctgaattagc tgtagaagct cgttatgata ttgaggctgt tttacctaag aaaataacct
      601 tcattcatac cgaagaattg gtagcgaaat accctgattt gacgcccaaa gaacgtgaaa
      661 atgccattac taaagaattt ggagcagtct ttttaattgg tattggcggg gttttacctg
      721 acggtaaacc acacgatggc cgcgctcctg actatgatga ctggacaaca gaaactgaaa
      781 atggctatca tgggcttaat ggtgatattt tggtttggaa tgaccaactt ggttcagcct
      841 ttgaattatc ctcaatgggg atccgtgtgg atgaagaggc cctaaaacgt caggtggaaa
      901 tgactggtga ccaagaccgc ttagcctttg attggcacaa atctttgttg aatggacttt
      961 tccctctaac gattggtgga ggaatcggtc aatcacgtat ggtcatgttt ttgttgcgta
     1021 aaaaacatat tggagaagtt cagacctctg tttggcccca agaagttcgt gatagctacg
     1081 ataatatttt ataagtaatc acatcatttt gctaagtttt ccacttgtat aagagacttt
     1141 aaataaatga tcagaaatat tgaaaaaaga aatcattttt aaggtttaga gctagaaatt
     1201 tgctataata aatgttatga gagttgtatc aggtgaattt ggtgggcgtc ctttaaagac
     1261 gctcgatgga aagataacac gccctacttc agacaaagtt agaggtgcga tttttaatat
     1321 gataggacct tactttaatg gtggtcgtgt tttagatttg tttgcaggtt caggtggatt
     1381 agccattgag gctgtttctc gtgggatgtc tgctgcagtt ttggttgaga agaatcgtaa
     1441 agcgcaagct attattcaag acaatattat aatgactaaa gctgagaatc ggtttacctt
     1501 gttaaaaatg gaagctgagc gagctattga ttgcttgact ggtcgatttg acttagtctt
     1561 tttagaccct ccttatgcca aagaaacaat tgtggcaact attgaagctt tagcagctaa
     1621 aaacttattg agtgaacagg ttatggttgt ctgtgagaca gataaaacag tcttgttgcc
     1681 aaaagagatt gcaacgttag gtatttggaa agaaaaaatt tatggaatta gtaaggtaac
     1741 agtttatgtt aactaaaata ggactttata cagggtcatt tgatcctgtt acaaacggtc
     1801 atctcgatat tgttaaacgg gcaagtggcc tatttgatca aatatatgtt gggatttttg
     1861 ataatcctac caaaaaaagc tattttaaac tagaagttcg taaagcaatg cttactcaag
     1921 ctctggctga ttttacaaat gtaattgtag taacttcgca cgaacgttta gctattgatg
     1981 tagctaaaga attacgagta actcacttga ttagaggttt gcgaaatgca actgactttg
     2041 agtatgaaga aaatttggaa tattttaatc accttttagc tccaaatatt gagaccgtct
     2101 atttgatatc aagaaataaa tggcaggccc ttagctctag tcgtgtgaga gagctaattc
     2161 attttcagtc ttccttagaa ggtttggttc ctcagtcggt tattgctcaa gtggagaaaa
     2221 tgaatgaaaa gacttaaaaa aatcaaatgg tggttagtgg gtctgctagc tttaatctct
     2281 ttgttgctag cgttattttt tccgctacct tattatattg aaatgcctgg aggcgcttac
     2341 gatattcgga ctgtcttaca agtcaatggc aaagaagaca aacgaaaagg ggcttaccag
     2401 tttgttgcag tgggcattag tcgtgccagc ctcgctcagc tattatatgc ttggctgaca
     2461 ccgtttactg aaattagtac agcagaagat acaacaggcg gatacagcga tgctgatttc
     2521 cttcgaatta atcaatttta catggaaaca tcacaaaatg cagctattta tcaagcttta
     2581 tccttagctg gaaaaccagt tacattagat tataaaggcg tatatgtttt agacgtaaac
     2641 aacgaatcta cttttaaagg aacgttacac ttagcagata ctgtaacagg tgtaaatggt
     2701 aaacagttta ctagttcagc agaacttatt gactatgttt ctcacctaaa actaggggat
     2761 gaagttacgg ttcagtttac gagtgataat aagcctaaaa aaggagttgg ccgtattatc
     2821 aaactgaaaa atgggaaaaa tgggattggc attgccttga ctgatcatac aagtgtcaat
     2881 tcagaagaca cagtgatctt tagtactaaa ggagtaggag gacctagtgc tggtctaatg
     2941 tttactcttg atatatatga tcaaataact aaagaagatt tacgcaaggg ccgtacaatt
     3001 gcaggtacag gaactattgg caaggatggc gaagtaggag atattggtgg tgcaggtctt
     3061 aaagtagttg cagcagctga agctggtgca gatatatttt ttgttccgaa taatcctgtt
     3121 gataaggaaa ttaaaaaagt taatccaaat gctataagta attacgaaga agccaaacgg
     3181 gcagccaaac gactaaagac caaaatgaag attgttccta ttacgactgt tcaagaggca
     3241 ctggtttatc ttcgcaaata aaataactat gaatgtatta tattcaatta tattcacgct
     3301 attaactcat aaaaaacggt tgaaccagat agacagatcc ttgaccagtc ttttgggttc
     3361 aatcattttt tacataaaac atatctaact tgacagcgta aaaaagatgt tataaaagtc
     3421 tagtggtaaa aaggcaatag agaattgagt aaactttagt cgattatctg gaaatagagc
     3481 atactaaaaa cacctagtga aaattgattt ttttcactag ctcatccaag aacttgcgca
     3541 gataatcatg agtccattcc atgactctat tattctggaa agccttgcaa tagacaagtt
     3601 aacgttataa caactaaatt aaaaacttga gattgaaaaa tccccataaa cttattttac
     3661 aaggaggtaa ggctatggta accattaaac aagttgctga agaagcaggt gtttctagat
     3721 caacagtctc caggtatatt tctcaaaaag gatatgtatc tgatgatgca aggcataaaa
     3781 tcaaagcagc gatagcaaaa ttacattaca ctccaaatgt cttggcgcag tcattgaaga
     3841 cgaaaaaaaa tcaactagtc ggtttattat taccagacat ttccaatcct ttttttccga
     3901 ggttagcaag gggagcagaa gaatatttaa aagaaaaggg gtatcgggtt atgctaggaa
     3961 atatttcaga cagcgaagct cttgaagaag aatacgtcca tgtccttctt caaagcaatg
     4021 ctgctggcat tattactacc catgacttta caaagcggta tcctactctt gctattccag
     4081 ttgtagtggt agatcgtgtt gaccaagaga cccagtacgg tgtcttttca gataataggg
     4141 cagggggctt attggcggct cagactgtct ggcaagcagg ggcaaaagaa gttttgttaa
     4201 taagaggtcc gctggataat gctgaaaaca ttaatgagcg gtttgaagct agtttttctt
     4261 accttcaaaa acaagacgtg actatgtatg tgtgtgatag tcagaatttt gattttgaaa
     4321 gtattcagct agaagctagt tacaacctca agtgttaccc aacaatagac agtattatag
     4381 caccttctga tatccatgct attgcttata ttcatgaact gcattctcaa ggtaaaaaga
     4441 ttcctcaaga tgttcaaata ataggttatg atgacatttt aatgagtcaa tttatttatc
     4501 cttctttatc gactattcat caatcctctt atttgatggg ccgttatgcc gccgaattag
     4561 tttatactat tgctagtcaa ttaactgtga aagcaaatcg gattaaattg cctgttcact
     4621 acgttgagcg agaaacaata aggaaaagat aagtaataat attcgtccag attttagcag
     4681 gtatgatgaa gatttgttgg gatttacctt ctactttaaa agatgttatc tgtcattact
     4741 ttcatcgcta tcagtcaaaa cgcattctta tgcaactctt aaccttaagc ttaaggtctt
     4801 tttgataaca tagaggaatg ttacacgatg atgacgcaat aaagcctaaa tatagtaatt
     4861 gtcgtgattt tatagctagt gagtaatgag atagcaagtc tctcatttac ttacaaaagt
     4921 caaaaaatag tgtataatgg ttgagttaca gataacactg caaaaaagat aacaagtgtc
     4981 cataattatc taagactata aaggcgatat agacagatat aaaaaagatg acgaatcagt
     5041 tatcactgga caattaaccg atttagttac aactaatgga tttttcagaa aggtgtcaaa
     5101 tgaaaaaaga aatttcgcca gaaatgtaca actataataa atttccaggt cccaaattta
     5161 ttcattttga agagcaagtt aaggctgaag gcattgattt gttactctta gaagatgtta
     5221 agaacgcttt tgatacgact agctttggtc aacgttatac agaagttctc ctaaagtatg
     5281 actatattgt tggcgattgg ggaaatgaac agcttcgtct taaagggttt tataaggata
     5341 gtgacgatat caagaaaacg aatcgcatct cacgtttaga agattatatt aaagaatttt
     5401 gcaattttgg ttgtgcttat tttgttttag aaaatcttca tccacaagat attaaatttg
     5461 aagaggagcg ccaaccaaga cgaaagaaat cacctaaatc aaaatcaaat cgtcgcaagc
     5521 caaactattc aaatcagcag cccgcaatac ctaagagtaa atcgaagcgg gtatcaaaag
     5581 aaaagcaacc tgaaaaccaa gcgtttacca gtcaaaaacg tcgaagtaat actaagcata
     5641 aagaaaagtc aaaacgtaat cagactagtc aacttaatac caaaattagt cattttatca
     5701 tcagaaagaa agataaataa tccatgaaac catcgattta tagcttaaca cgcgatgaac
     5761 tgatcgcgtg ggcagttgaa cgcggccaaa aacagtttag agctactcaa atttgggatt
     5821 ggctctataa aaaaagagtt caatcttttg aggagatgac gaatatttct aaagattttg
     5881 tttctatttt aaatgatagt ttctgtgtca atcctttaaa acagcgagtt gtacaagaat
     5941 cagctgatgg tacagtaaaa tatttatttg aactgcccga tggtatgtta attgaaacag
     6001 ttttaatgcg tcaacattat ggtcattctg tttgtgtgac gactcaagtt ggctgtaaca
     6061 ttggctgtac tttttgtgct agtggattga tcaaaaagca acgtgatcta aacagtgggg
     6121 aaattacagc tcaaatcatg ttagtccaaa aatattttga cgatagaaag caaggtgagc
     6181 gggttagcca tgttgtcgta atgggcattg gtgaaccttt tgataattac aaaaatgtca
     6241 tgtgctttct tcgcgtcatc aacgatgata atggcttagc aattggtgcc cgtcatatta
     6301 cagtctctac ctcagggtta gctcacaaga ttcgggattt tgcaaacgag ggagtacagg
     6361 ttaatttagc agtctcgttg catgcaccaa ataatgattt acgttcaagt attatgcgag
     6421 taaatcgctc ttttccgctg gaaaagctct tttcagctat agaatactat attgaaaaaa
     6481 cgaatcgtcg tgtcactttt gaatacatta tgctaaatga agttaacgat agcattaagc
     6541 aagctcaaga actagcagac ttaactaaaa ctattcgtaa gttatcttat gttaacttga
     6601 ttccgtataa tcctgtttca gaacatgatc agtatagtag aagtcctaaa gagcgagttc
     6661 tagcgtttta tgatgtgcta aagaaaaatg gagttaactg tgtggtgcgt caagagcacg
     6721 gaacagatat tgatgcagcc tgtggccagt tacgttctaa aacgatgaaa aaagatcgcg
     6781 aaaaggttac agcgacaaaa taaagtccaa aaaatatctt tgattgacaa tgcttgatga
     6841 caaaatattc ctagttacta aaatgactag gttttttatc tggcctaaaa atgctctaga
     6901 agcacaatcc aaaataatcc aaaactaaga taaggaataa aaggtatgcg cttgatgcct
     6961 aatagtaagc aagcagtaat acctgctaaa ctagcaagtt gaattagcca aatcaacgac
     7021 gttagatcca atactaaagc taagctagca aggtagaaaa aatcgcctgc tccaattgaa
     7081 aaaggtctta aatgagctaa taaacctagt agcagtaata tcaatgataa ataattgatc
     7141 gaataaaaca ggagtagact tacaaagcta aaaagccaca agataaaagg gtattcttga
     7201 gaacgccaat caaataaact aaataataaa gaaaagagta acaagtaaca gtaactagca
     7261 ctaacccaat gtaccatact agcccataag ataattatta gacttatctc taagctaaag
     7321 aagatttttt gactagttat aaaatggttt tggagctgcc gaaaaacgcg tttggcaaaa
     7381 gttagttggt cacaaacaat gacagtctgt ttacttagct gttcttgacc attatcagga
     7441 aaataaagta gtaaacagta aaaaagatgg gtcaataaga tgcccgaaca tatactaaaa
     7501 aaagctatca tacttattta ttcgaaaaga aatcaaatac ttgcaatctt tccttaatca
     7561 ttgtagaata tagatataaa acgataatga ttctaattaa gtgtctaatc atccaattag
     7621 gattattccg ttattgtcga ataggattag gtccttaacc ctttcttatg tttatcacgt
     7681 tagggttagt tccccctcca ttcttgccct ttaagctgaa aagtaaggag atgttattat
     7741 gacaaacaca ctcgttgaaa atatttatgc ttcagtaact cacaatattt ctaaaaaaga
     7801 agcatcaaaa aatgaaaaaa ctaaagctgt tttaaaccaa gctgtcgctg acttatctgt
     7861 ggctgcatct attgtgcacc aagtacactg gtatatgcgt ggtccaggtt tcttgtacct
     7921 tcaccccaaa atggatgaat tacttgatag cttgaatgct aatttagatg aaatgagcga
     7981 gcgtttgatc actattggcg gagcgcctta ttctacccta gctgagtttt ctaaacactc
     8041 taaattggac gaagcaaaag gtacttacga taaaacagtt gctcagcatt tagcaagatt
     8101 ggtcgaagtt tacctttacc tcagcagcct ttaccaagtt ggtttggata ttaccgatga
     8161 agaaggcgat gctggaacaa acgatttgtt tacagcagct aagacagagg ctgaaaaaac
     8221 catttggatg ctccaagcag agcgtggaca aggcccagca ctctaattga ttatgcctta
     8281 ggataaaaga agtcaatgat aaaattggct tcttttttat ggaatcatcg attcattttc
     8341 agaaacatag aaagcgattc cataattgtc ttgaaatctg ccgtagaatt tgctaaaatc
     8401 tacttatatg aaaacactat atgatgtgca acaattgcta aaaaactttg gcatttttgt
     8461 ttaccttggc aaacgtcttt atgatattga aatgatgaag attgaacttc agcgtctcta
     8521 tgatagcggc ttattggata agagggatta cttaaatgcg gagttaattt tgcgacgtga
     8581 gcatagatta gaattggaaa aagaaggata gacaaatgag tcaaaaatta ctggggattg
     8641 atttaggtgg taccaccatt aaatttggaa ttttaacagc agcaggagaa gtccaagaaa
     8701 aatgggcaat tgagactaat attttagaag gaggcaaaca tattgttcct gatatcatcg
     8761 cttccatcaa acatcgctta gacttgtatg gtctaagcag tgctgatttt gtgggaattg
     8821 gtatgggatc accaggagcg gttgaccgtg atactaatac agttactggg gcattcaacc
     8881 ttaattggaa agaaacccaa gaagtcggtt ccgttgttga aaaagaattg ggcattcctt
     8941 ttgccattga caatgatgcc aatgtggctg cccttggtga acgttgggta ggtgctggtg
     9001 aaaataaccc agatgtcgtc ttcatgacac ttggaacagg tgtcggtgga ggcattattg
     9061 ctgatggtaa cttgattcat ggtgttgcag gagcaggtgg tgaaatcggc cacatgattg
     9121 ttgagccaga aaatggcttt gcttgtactt gtggctcaca cggctgtttg gaaacagtag
     9181 cttcagcaac aggagttgtc aaagtggcac gtttactggc agaagcctac gaaggggatt
     9241 cagccatcaa agcagctatt gacaatggtg aaggtgttac cagtaaagac attttcatgg
     9301 cggctgaagc aggggattcc tttgcttatt ctgttgtgga aaaggttggt tactaccttg
     9361 gccttgcttc agcaaatatt tccaatatct tgaatccaga ctcagtggtt atcggtgggg
     9421 gtgtgtcagc agcaggagaa tttctacgct cacgtattga aaaatacttt gtgaccttca
     9481 ctttcccaca agtgaggtat tcaactaaaa ttaagattgc agagcttggc aatgatgctg
     9541 gaattatcgg tgcagctagc cttgctcgac aatttatcac aaaataggag ggggatatgt
     9601 ctccaatcac acttatctta tggttacttc ttgtcggcat tgttggttat tatacatgga
     9661 actatttctc ttttcgaaaa atggcaaagc aggttgataa cgagactttt aaagacgtga
     9721 tgcgtcaagg tcaattgatt gatttacgag agccagcggc ttttagaact aagcatattt
     9781 taggagctcg aaattttcca gcgcaacaat ttgatgctgc tattaaggga ctacgcaagg
     9841 acaaacctgt tcttatttac gaaaatatgc atccccagta ccgtgtgcct gcggtcaaaa
     9901 aacttaaaaa agctggcttt gaagacgttt atgtcttaaa agacggtatt gattattggg
     9961 atggcaaagt taaacaaaca acctagttaa gagtaacgtg gagttaaggg gaataaagac
    10021 agtcactgtc tcaaaaacct taattccttt ttttgctgta tccagacttg ctgaaagtct
    10081 gaaaacattt acaattgatt aaaaccagtt ttttaaaaca ttttgtgtta tacttatcta
    10141 gttaaaatat atttacttag aggaacaaat gactaactta agaaacgata tccgtaacgt
    10201 agcgattatt gcccacgttg accacggaaa aacaacactt gtagatgaat tattaaaaca
    10261 atcccatact cttgatgagc gtaaagagct ccaagagcgt gccatggatt ccaatgacct
    10321 tgaaaaagaa cgtgggatta caatccttgc gaaaaatacg gcagtagcct ataacgatgt
    10381 tcgtattaac atcatggata ccccaggaca cgcggacttt ggtggtgaag ttgaacgtat
    10441 catgaaaatg gttgacgggg ttgttcttgt tgtggatgcc tacgaaggaa caatgcccca
    10501 gacgcgtttc gtattgaaaa aagcacttga gcaaaacctt atcccaatcg ttgtggtgaa
    10561 caagattgac aaaccttcag ctcgtccagc agaagttgta gatgaagtgc ttgaattatt
    10621 catcgaactt ggtgccgatg atgagcaatt ggaattccca gttgtttacg catcagctat
    10681 taatggcaca tcatcattat cagatgatcc tgctgaccaa gagcatacta tggcacctat
    10741 ctttgatacg attattgatc atattccagc gccagttgat aattcagatg agcctttgca
    10801 attccaagtg tcacttttgg actacaacga tttcgtaggt cgtatcggta tcggtcgtgt
    10861 tttccgtggt actgttaaag ttggtgacca agtaactctt tcaaaacttg atggtaccac
    10921 taaaaacttc cgtgttacaa aactgtttgg tttcttcggt ttggaacgtc gtgaaattca
    10981 agaagctaaa gcaggtgact tgattgctgt ttcaggtatg gaagatatct ttgttggaga
    11041 aaccattaca ccaactgact gtgtggaagc tctgccaatt cttcgtattg atgagccaac
    11101 gcttcagatg actttcttgg tcaataactc tccttttgca ggtcgtgaag gtaaatggat
    11161 cacgtcacgt aaggttgaag aacgtctttt agcagaattg caaacagacg tgtcacttcg
    11221 tgttgaccca acagattcgc cagataagtg gacggtttca gggcgtggag aattgcactt
    11281 gtcaattctt atcgaaacca tgcgccgtga aggctatgaa cttcaagtat cacgtccaga
    11341 agttatcatc aaagaaattg atggtgtcaa atgtgaaccg tttgagcgtg ttcaaattga
    11401 tacaccagaa gaatatcagg gtgcaatcat ccagtctctt tcagaacgta aaggggatat
    11461 gcttgatatg cagatggttg gtaatggtca aacgcgtttg attttcttga ttcctgcacg
    11521 tggtttgatt ggttattcaa cagagtttct ttcaatgaca cgtggatatg gtatcatgaa
    11581 tcatactttt gatcagtatc taccggttgt tcaaggagaa attggtggtc gtcatcgtgg
    11641 tgccttggtt tctattgaaa atgggaaagc aacgacatat tcaatcatgc gtattgaaga
    11701 acgtggaact atctttgtca acccaggtac agaagtttac gaagggatga ttgttggtga
    11761 aaactcacgt gataacgacc ttggtgttaa cattacaaca gctaagcaaa tgacaaatgt
    11821 ccgttcagcg actaaagatc aaactgcagt tatcaagacg cctcgtatcc taacgcttga
    11881 agaatcatta gagttcttga atgacgacga atacatggaa gtaacgccag aatctatccg
    11941 cttgcgtaaa caaatcttga ataaagctgc gcgtgataaa gctaataaaa agaaaaaatc
    12001 agccgaatag cttttgaagt taaactaact aaataaaata gcaaaaagat atttaagaaa
    12061 agtcattttt taaaggtctt tttgcgttta caagttccta tggaatataa accaaaataa
    12121 aggaggacgg aatgttttac cttattatgg ctgtcttgat tatatcttat tatctataca
    12181 tggctcctaa aagtgtccgt aacacccttg ggatgattgg tttagttggc ttggttgctc
    12241 tgcttattgt tttagcgggc ttgagtttta tcaaaatcat gcaaacccct cctgaatttt
    12301 ttattgggat gggaatggtg gcattgggct attttgcttt aaaagatgtg cgtaaaatga
    12361 ccaaaaaacc gagagttaaa taaaagtact taaagattga tgattttggt ttttatacga
    12421 taaaaggact gatttgtttt agacaatatc agtccttttt taatagttca ttttttctga
    12481 aagaaagctt ttagccgatt ttcattgagt gtgcaactaa aaagagatgt ttggtaataa
    12541 tagttaagac ctctgactag tattttatct tattttactt aaaaaaacgt accaataatc
    12601 tcacaaatat gggcatgatg tttgtatatt ggaaacggat tctaattctc aactcaagaa
    12661 cggtattttt aaaatgttga aaaagcatct aaataaaggt aaaatagaaa tcaaatagaa
    12721 tggatagtgt gagcttagat gaaagtgata agtaattttc aaaacaaaaa aatattaata
    12781 ttggggttag ccaaatcagg cgaagcagca gcaaaattat tgactaaact tggtgcttta
    12841 gtgactgtta atgatagtaa accatttgac caaaatccag cggcacaagc cttgttggaa
    12901 gaggggatta aggtcatttg tggtagccac ccagtagaat tattagatga ggactttgag
    12961 tacatggtta aaaaccctgg gattccttat gataatccta tggttaaacg cgcccttgca
    13021 aaggaaattc ccatcttgac tgaagtagaa ttggcttatt tcgtatctga agcccctatt
    13081 atcgggatta caggatcaaa cgggaagaca accacaacga caatgattgc cgatgttttg
    13141 aatgctggcg ggcaatctgc actcttatct ggaaacattg gttatcctgc ttcaaaagtt
    13201 gttcaaaaag caattgctgg tgatactttg gtgatggaat tgtcctcttt tcaattagtg
    13261 ggagtgaatg cttttcgccc tcatattgct gtcatcacta atttaatgcc gactcacctg
    13321 gactatcatg gcagttttga ggattatgtt gctgctaaat ggatgattca agctcagatg
    13381 acagaatcag actaccttat tttaaatgct aatcaagaga tttcagcaac tctagctaag
    13441 accaccaaag caacagtgat tcctttttca actcaaaaag tggttgatgg agcttatctg
    13501 aaggatggta tactctattt taaagaacag gcgattatag ctgcaactga cttaggtgtc
    13561 ccaggtaggc acaacattga aaatgcccta gcaactattg cagttgccaa gttatctggt
    13621 attgctgatg atattattgc ccagtgcctt tcacattttg gaggcgttaa acatcgtttg
    13681 caacgggttg gtcaaatcaa agatattacc ttctacaatg acagtaagtc aaccaatatt
    13741 ttagccactc aaaaagcttt atcaggtttt gataacagtc gcttgatttt gattgctggc
    13801 ggtctagatc gtggcaatga atttgacgat ttggtgccag accttttagg acttaagcag
    13861 atgattattt tgggagaatc cgcagagcgt atgaagcgag ctgctaacaa agcagaggtc
    13921 tcttatcttg aagctagaaa tgtggcagaa gcaacagagc ttgcttttaa gctggcccaa
    13981 acaggcgata ctatcttgct tagcccagcc aatgctagct gggatatgta tcctaatttt
    14041 gaggttcgtg gggatgaatt tttggcaacc tttgattgtt taagaggaga tgcctaatgc
    14101 ctaagaagat tttatttaca ggtggtggaa ctgtaggtca tgtcaccttg aacctcattc
    14161 tcataccaaa atttatcaag gacggttggg aagtacatta tattggtgat aaaaatggca
    14221 ttgaacatac agaaattgaa aagtcaggcc ttgacgtgac ctttcatgct atcgcgacag
    14281 gcaagcttag acgctatttt tcatggcaaa atctagctga tgtttttaag gttgcacttg
    14341 gcctcctaca gtctctcttt attgttgcca agcttcgccc tcaagccctt ttttccaaag
    14401 gtggttttgt ctcagtaccg ccagttgtgg ctgctaaatt gcttggtaaa ccagtcttta
    14461 ttcatgaatc agatcggtca atgggactag caaacaagat tgcctacaaa tttgcaacta
    14521 ccatgtatac cacttttgag caggaagacc agttgtctaa agttaaacac cttggagcgg
    14581 tgacaaaggt tttcaaagat gccaaccaaa tgcctgaatc aactcagtta gaggcggtga
    14641 aagagtattt tagtagagac ctaaaaaccc tcttgtttat tggtggttcg gcaggggcgc
    14701 atgtgtttaa tcagtttatt agtgatcatc cagaattgaa gcaacgttat aatatcatca
    14761 atattacagg agaccctcac cttaatgaat tgagttctca tctgtatcga gtagattatg
    14821 ttaccgatct ctaccaacct ttgatggcga tggctgacct tgtagtgaca agagggggct
    14881 ctaatacact ttttgagcta ctggcaatgg ctaagctaca cctcatcgtt cctcttggta
    14941 aagaagctag ccgtggcgat cagttagaaa atgccactta ttttgagaag aggggctacg
    15001 ctaaacaatt acaggaacct gatttaactt tgcataattt tgatcaggca atggctgatt
    15061 tgtttgaaca tcaggctgat tatgaggcta ctatgttggc aactaaggag attcagtcac
    15121 cggacttctt ttatgacctt ttgagagctg atattagttt cgcgattaag gagaagtaaa
    15181 tggcaaaaga taaagagaaa caaagtgatg acaagctcgt tttgacagag tggcaaaagc
    15241 gtaacattga atttttaaag aaaaagaagc agcaagctga ggaagaaaaa aaactcaaag
    15301 agaaattatt gagtgataaa aaagcgcagc agcaagctca aaatgcttct gaagcagttg
    15361 agcttaaaac tgatgagaaa actgatagtc aggaaattga gtcagaaacg acgacaaaac
    15421 ctaaaaaacc caaaaaagtt agacaaccca aggaaaaaag cgcgacacaa atcgcttttc
    15481 aaaaatcctt gcctgttctt ttgggggcgc tcttactcat ggcggtgtct atttttatga
    15541 tcactcctta tagcaaaaag aaagagtttt ctgtaagagg aaaccatcaa acgaaccttg
    15601 acgaattaat caaagctagc aaagtcaaag catctgacta ttggttaacg ttgttaactt
    15661 cgcctggtca gtatgaacga ccgattcttc gtactattcc atgggtgaaa tctgtacatc
    15721 tctcttacca atttcctaat cactttctat ttaacgttat tgaatttgaa atcatcgctt
    15781 atgcacaagt cgaaaacggt tttcagccta ttttggagaa tggaaaacgt gttgacaagg
    15841 tcagggcatc agaactaccg aaatctttct tgattcttaa tttaaaagat gagaaagcga
    15901 tccaacagtt agttaagcaa ttaacgacat tacctaaaaa attagtcaag aatatcaagt
    15961 cagtgtctct tgcaaattcc aaaacgacag cggatttact acttattgaa atgcatgacg
    16021 gtaatgtagt tagagtaccg cagtcacaac tcacattgaa acttccctat tatcaaaaat
    16081 tgaaaaaaaa ccttgaaaat gatagtatag tggatatgga agttggaatt tatactacaa
    16141 cacaggagat tgaaaatcaa cctgaagttc ctcttacgcc agaacaaaac gcagctgata
    16201 aagaaggaga taagcctggt gaacatcagg aacagacaga caatgattca gagacgccag
    16261 caaatcagag tagtcctcag caaacaccac catccccaga aacggtcctc gaacaggctc
    16321 atggctagct aatatctaag ttgaaaaagc aatgaaaacg ttagaaattc aacgattcta
    16381 acccataatg aattgcctaa aaaaaattaa gtttatataa caaaaaacgt aaaatgataa
    16441 cattttacgt ttttttatgg tataatattt tctgaatgat tctgtttttt agcagttttt
    16501 agaatagcaa aagtttggaa agtaagtgag gtcaagtgaa tggctagaaa tggctttttt
    16561 actggtttgg acattggaac aagctcgata aaagttttag tagcagaatt tatttctggt
    16621 gagatgaacg tcattggtgt tagtaatgtt ccaagtaccg gcgtaaaaga tggcataata
    16681 atcgatatag aggcagctgc gactgccatc aaaactgcgg tagaacaagc agaagaaaaa
    16741 gcagggatga caattgaaaa ggttaatgtt gggctaccgg caaaccttct tcaaattgaa
    16801 ccaacacaag gaatgattcc tgtcccaagt gagtctaaag agataaaaga tgaggatgtt
    16861 gatagcgttg ttaaatcggc tttaacaaaa agtatcacac cagaacgaga ggttatctct
    16921 ttagttccag aagagttcat tgtggatggc tttcagggca ttcgagatcc acgtggtatg
    16981 atggggatta gattagagat gcgcgggctt atttatactg gaccaagcac cattttacat
    17041 aatctgcgta aaacggtaga aagagcaggc attaaagttg aaaacatcat tatttctccg
    17101 ttagctatgg ctaaaaccat tttaaacgaa ggtgagcgcg agtttggagc tactgtaatt
    17161 gatatgggag gtggacagac aactgtcgct tctatgcgag cacaagaatt gcagtatacc
    17221 aatatatatg ctgaaggcgg cgaatacatt actaaagata tatcaaaagt attaaaaacg
    17281 tctttggcta ttgcagaagc acttaagttt aattttggtc aagcggagat atcagaagct
    17341 agtataactg aaacagtaaa agttgatgtg gtaggtagtg aagagcctgt tgaggtaact
    17401 gaacgttatt tatctgaaat tatttcagcg cgtattcgtc atattttaga tcgtgtgaag
    17461 caagatttgg aaagaggtcg tttactagac ttaccaggag gcattgtttt gattggcggc
    17521 ggtgcaatca tgcctggagt ggtagagatt gcacaagaaa tctttggagt aactgtaaag
    17581 ctccatgttc caaatcaagt cggtattaga aatccaatgt tttcaaacgt tatcagtttg
    17641 gtagaatatg ttggtatgat gtctgaagta gacgttttag cacaaactgc agtttcagga
    17701 gaagaacttt tgcgacgcaa gcctatcgat ttcagtggtc aagaatctta tttaccagat
    17761 tatgatgatt caagaagacc agaatcgacc attggctatg aacaacaagc gtcacaaaca
    17821 gcatatgatt cacaagttcc gagtgatcct aaacaaaaaa tttcagaacg tgttcgtggc
    17881 atatttggga gtatgtttga ttaaaagtaa taaagtgagg agataaaatg gcattttcat
    17941 ttgatactgc atcaattcaa ggtgcaatta taaaagtaat tggagtcggc ggaggtggcg
    18001 gaaatgccat taatcgtatg attgatgaag gtgttgctgg tgtcgagttc atcgcagcaa
    18061 acacagacat tcaggcatta agctcatcaa aagctgaaac ggttattcaa ctaggcccta
    18121 aattaactcg tggacttggt gctggaggac aacctgaagt aggacgtaaa gctgctgaag
    18181 aaagcgaaga aattttaaca gaagctctta caggagcgga catggtattt attactgccg
    18241 gtatgggtgg tggctctggg acaggggctg caccggttat tgctcgtatc gctaaaagtt
    18301 tgggagcctt gacagtagct gttgttactc gcccgtttgg ttttgaaggt aacaaacgtg
    18361 gtaattttgc tattgaaggt atcgaagaac tccgtgaaca agttgatact ttgttaatta
    18421 tttcaaataa taaccttctt gagattgttg ataaaaagac acctttatta gaagcactta
    18481 gtgaagctga taatgtttta cgtcagggag ttcaagggat aaccgactta attactagtc
    18541 ctggccttat caatctcgat tttgccgacg tgaaaacagt tatggcaaat aaagggaatg
    18601 ccttgatggg gattgggatt ggttctggag aagagcgcat tgttgaggcg gcgcgtaagg
    18661 caatctattc acccctatta gaaacgacta ttgatggtgc acaagacgtt attgtgaacg
    18721 ttacaggagg tctcgacatg acactcacag aagctgaaga agcctctgaa attgttgggc
    18781 aagctgctgg tcaaggcgtt aacatttggt taggaacatc tattgatgat actatgaaag
    18841 atgacatccg tgtgactgtt gtagcaactg gagtgcgcca agaaaaagcc gaacaagttt
    18901 caggttttcg tcagcctagg acttttaccc aaaccagcgc gcagcaagta gcgggtgcac
    18961 aatatgcatc agatcaagca aaacagtcgg ttcaaccagg gtttgatcgt cgctcaaatt
    19021 ttgattttga catgggggag tctcgcgaga taccaagtgc acaaaaggta atttctaatc
    19081 ataatcaaaa tcaaggttca gcttttggaa attgggattt gagacgtgat aatatttctc
    19141 gtccaacaga aggtgaattg gataaccatc ttaatatgtc aacgttctca gctaacgatg
    19201 acagtgatga tgaattagaa acgcctccat tctttaaaaa ccgttaataa tggatttact
    19261 gacaaataaa aagaaaattt ttgagactat ccgcttatct acagaggcag caaataggac
    19321 taatgatagt gtttcagtta ttgctgtaac aaaatatgtg gatagtacaa ttgcaggtca
    19381 gcttatcgaa gcaggaattg agcacattgc cgaaaaccgt gttgataaat ttcttgaaaa
    19441 gtatgatgcg ttaaagtata tgccagtaaa gtggcattta attggtacct tacaacgtcg
    19501 taaagtcaag gaagttatca attatgtcga ttattttcac gctctagatt ctgtgagatt
    19561 agctttggaa atcaacaaga gagctgacca tcctgtgaag tgttttctac aagttaatat
    19621 ttctaaagaa gagagtaaac atggttttaa catttctgag attgatgaag cgattgaaga
    19681 aataggtaag atggagaaga tacagttagt tggtttaatg actatggcac cagcaaatgc
    19741 cagtaaagga agtattataa ctatttttcg acaagcaaat caattaagaa aaaacttgca
    19801 gttaaaaaaa agaaagaata tgccttttac agaattgagc atgggcatga gtaacgatta
    19861 tccaattgct attcaagaag gctcaacttt tattcggatt ggtagagctt tctttcacta
    19921 atggagaata agatggcttt taaagataca tttaacaaga tgatttctta ttttgacacg
    19981 gatgaggtta acgaagttga agaagatgtt gcagcatcaa ctgataacgt tattccaaga
    20041 tcacaacaat cagtcagagc aagtagtcat ccaaaacaag aacctagaaa caatcacgta
    20101 caacaagatc atcaagcgag atcccaagaa cagacaaggt cacaaatgca tccaaaacat
    20161 ggtacttctg aacgctatta tcagcagtct cagccaaaag aaggccatga aatggttgac
    20221 agaagaaaac ggatgaacac ttctggtatt gcaaatcgcc gtgagcagta tcaacaatca
    20281 acttgttcag atcaaacaac tattgcctta aagtatcctc gtaaatatga ggacgctcaa
    20341 gaaattgtgg atcttttaat agttaatgaa tgcgttttga ttgattttca gtttatgcta
    20401 gatgctcagg ctagacggtg tttagatttt attgatggtg ctagtaaagt gctctatggt
    20461 agcttacaaa aggtcggctc ttcaatgtac ttactggctc cgtcaaatgt atccgtcaat
    20521 atagaagaaa tgactatccc acatactaca caagatattg gctttgattt tgatatgaaa
    20581 aggcggtaaa taaatgatat taatactatc tattcttctt cgtctgatca aagtttacac
    20641 ttatttattg attgcttacg cactaatgtc atggtttcct ggggcatacg attcaaaaat
    20701 tgggcgtttg attagtggta tcgttgaacc aattttaaaa ccttttagag catttaattt
    20761 acaatttgcc ggtcttgact tcactatttt tgtcgtcatt attagtttga attttttagc
    20821 tcaagttttg gtccgtgtgt ttatttaatg gttagtcata gtaagattta tcagcatttt
    20881 caccaagaag aatatccttt tattgataga atgtctgata tgattaatag agttgaagat
    20941 tactatcttt tagaagttac tgagttttta aatcctagag aagtgatgat tttaaaaagt
    21001 ttgattgctt taacagatct aaaaatgttc gtatcaacag attactaccc aagcgaatat
    21061 ggtcgtgtca ttattgcacc tggttactat gacttagaac aaagtgattt tcaaatagct
    21121 ttagtagaga taagttatca ggcaaagttt aatcagttga cacatagtca aattttagga
    21181 actttaatta atgaattagg agtaaagcga aatttatttg gagatgtttt tgttgaaatg
    21241 ggatatgccc agctcatgat taagcgggag ttattggatt attttttagg aacaattact
    21301 aaaatagcta aaactagtgt gaaattaaga gaagttaact ttgatcagtt aattaggtct
    21361 attgataaca gccagaccct ggatattcta gtttctagtt ttcgattaga tggtgtagtt
    21421 gctactatct taaaaaaatc tcgaacgcaa gttatagcat taattgaagc aaataagatt
    21481 aaggtaaact atcgactcgc taataaagct tcagataatc tagtcatagg ggatatggtg
    21541 agtatcagag gtcacgggcg ttttactctt ttagcagata atggagtgac caaacatggc
    21601 aaacaaaaaa taacactaag taaaatgata cataaataag gagatacaat ggcacttaca
    21661 acgctagaaa ttaaagataa aacttttaag acaaaattcc gaggatactg tgaagaagaa
    21721 gtcaatgaat tccttgatat tgttgttgat gattacgaag ctcttgtacg taaaaatcgt
    21781 gataacgaag caagaattaa agatcttgaa gaaaaattat cttactttga tgaaatgaaa
    21841 gagtcactta gccagtctgt aatcttagct caagaaacag ctgaaaaagt gaaagctacg
    21901 gctaatgcgg aagcaacaaa cttggttagt aaagcgactt atgatgctca gcatttatta
    21961 gatgaatcta aagctaaagc caatcaaatg ttgcgtgatg caactgatga ggctaagcga
    22021 gttgcgattg aaacagaaga attaaagcgt caaacacgtg tgttccacca acgtttaatc
    22081 tcatctattg agtcacaatt aagcttatca aattcacctg aatgggatga actgctacaa
    22141 ccgactgcaa tctatcttca aaattctgac gatgctttta aggaagtcgt gaagaccgtt
    22201 cttaatgaag acattcctga atctgatgat agtgcctctt ttgatgcaac acgtcagttt
    22261 actccagaag agttagaaga attgcaacgt cgtgttgatg aaagcaataa ggagttagag
    22321 gcttaccaac ttgactctca atctgattct acgactgagc cagaggtaaa tctcagtgaa
    22381 acacaaacgt ttaaattaaa tatctaaata aagaaaaaca agatgatttg cttataatca
    22441 cagcaagcgg gagatgatga gagtctcgta ttccctaagt attatcatta tcattttcta
    22501 aaattttcgc tgaactacag taagcgagga ggtttgttca ccttacgagc aaagagggag
    22561 cggtaagtct tatgatatat tatcgctctg aactaaggtg gtaccacgag ctttcgtcct
    22621 ttttgagacg aggctttttt cgttatagtc gcaagtttgt ttaaaaggag tttgaaataa
    22681 tgaaattaaa agagacactt aatctaggaa aaactgcctt tccaatgcgt gcaggtcttc
    22741 ctaataaaga gccacaatgg caagcagctt gggaacaagc agagctttac aaaaaacgtc
    22801 aagaattgaa tgcaggtaag cctgcctttc atcttcatga tggacctcct tacgctaacg
    22861 gcaatattca cgttgggcat gcccttaata aaatttcaaa agatatcatt gtgcgttcta
    22921 agtccatgtc tggctttcaa gcgccttatg taccaggttg ggacacacac ggccttccaa
    22981 tcgaacaagt cttggctaag caaggaatca aacgtaagga gatggattta gcagaatacc
    23041 ttgagatgtg tcgtcaatac gctttaagtc aggttgataa acagcgaaat gatttcaaac
    23101 ggttaggtgt ttcggcagac tgggaaaatc cttatgtcac tttggaccca cagtttgaag
    23161 ctgatcaaat tcgtgttttt ggagcgatgg ctgaaaaagg ttacatctat cgtggtgcta
    23221 aacctgttta ttggtcatgg tcttcagaat cggccttggc agaagctgaa attgaatacc
    23281 atgacatcga ttcaacctct ttatactacg ccaataaagt taaagatggt aaaggcatcc
    23341 ttgatactaa tacgtatatc gttgtttgga ccacaacacc atttaccgtt acagcatcac
    23401 gcggtttgac tgttggtcct gatatggatt atcttgtggt gaaacctgct ggttcagacc
    23461 gtcaatatgt tgtggcagaa ggtcttttga atagccttgc tggaaaattt ggctgggaat
    23521 cttttgaaac tttagccagc cataaaggag ctgatttaga gtacattgtg acagaacacc
    23581 catgggatac tgacgtagaa gaattggtta tccttggtga ccatgttacc cttgagtcag
    23641 ggacagggat tgtccacacg gcgccaggtt ttggtgagga tgactacaat gttgggacaa
    23701 aatacaaact ggaagttgct gtgactgttg atgaacgtgg cctaatgatg gaaaatgcag
    23761 gtccagattt ccacggacaa ttttataaca aggtaacgcc gattgtcatt gataaacttg
    23821 gtgatctttt attggcacaa gaagtgatca atcactctta cccatttgac tggcggacga
    23881 aaaaaccaat catctggcgt gctgtaccgc aatggtttgc ttctgtttct gatttccgtc
    23941 aagatatttt ggatgaaatt gaaaaaacaa ccttccatcc atcatggggt gaaacacgcc
    24001 tttacaatat gattcgtgat cgtggcgact gggttatttc tcgtcaacgt gcttggggag
    24061 ttccgcttcc tatcttctat gccgaagatg gcacagctat tatgactaaa gaagtgacag
    24121 atcatgtggc tgatttgttc caagaaaatg gatcaatcat ttggtggcaa aaagaagcta
    24181 aagacctatt accagagggc ttcactcatc cagggtcacc aaatggcgag tttacaaaag
    24241 aaactgatat catggacgtt tggtttgact ctggttcttc ttggaatgga gtcatgaatg
    24301 ctagagaaaa tctttcctat ccagcggatc tctacttaga aggctctgac cagtatcgtg
    24361 gttggttcaa ttcatcctta attacatctg tggctgttaa tgggcatgct ccatataagg
    24421 ctattttatc gcaaggcttt gttcttgatg gtaagggcga aaaaatgtct aaatcaaaag
    24481 gcaatatcat ttctcctaat gatgtggcta aacaatatgg tgcagatatt cttcgacttt
    24541 gggtagcttc tgtggatacc gacaatgatg tccgtgtttc tatggagatc cttggtcaag
    24601 tctctgaaac ctaccgtaaa atccgtaaca cccttcgttt cttgattgct aatacatctg
    24661 atttcaaccc tgctacggat acagtagctt atgcagactt aggagctgtt gataagtaca
    24721 tgacaattgt ctttaatcag ttggtagcaa cgattactga cgcttatgag cgttatgact
    24781 ttatggctat ttacaaagca gtggtgaact ttgttacggt tgatttatca gccttctatc
    24841 ttgattttgc taaagacgtt gtctatattg aagctgccaa tagcttagaa cgtcgtcgca
    24901 tgcagacagt cttctatgat atcttggtta agattaccaa gttattgacg ccaatcttgc
    24961 ctcacaccac tgaagaaatc tggtcttatc tagaacatga gtcagaagca tttgttcaat
    25021 tggcagaaat gcctgtggca gaaactttct cagctcaaga ggatatttta gaagcttggt
    25081 ctgctttcat gactttgcgt acccaagctc aaaaagcttt ggaagaagcg cgaaatgcta
    25141 agattatcgg taaatcattg gaagcccatt tgaccatcta cgctagcgaa gaagtgaaaa
    25201 ccttattgac tgctttagac agcgatattg ctttgctttt gatcgtgtct caattaacca
    25261 ttgctgactt ggcagatgcg cctgcggatg cagtggcatt tgaaggtgtt gcctttatgg
    25321 tagaacatgc cataggtgaa gtttgtgagc gttcacgtcg catcgaccca actactcgca
    25381 tgcgttctta caatgcattt gtctgtgatc acagcgctaa aatcattgaa gaaaatttcc
    25441 cagaagctgt ggctgaaggg tttgaagaga gtggcaaata agtcttatca agacttgaag
    25501 tgacactgag taagaagaca accatcgggt cttaggacct gatggttttt tagtagagct
    25561 gattacgtcc attagccagc tttaacgaga gacaaagtag gagaaattta gactactttt
    25621 ttaatttctt ttaagaaaat tgctggcttc atataaggac tattaagata gagttcacct
    25681 ttcgctaata aaaaaactta gctcttttag aagggctaag ttttgtctga atagctggct
    25741 gttgtttata cagaagtttg aaatgaaatt tcaataaggt tattcgtgtg aagtgttttt
    25801 gattgtccag gaataacaat tttaggatca attttacgtt ttaaaaaaaa ttcgcgaatg
    25861 atatcaagtt catcctcctt gatggcacgg tatttatttg aaataagtag ttcgtaatgt
    25921 tttggtgctt gggtaaaaat gacatctgta ttccctgctg agtatacttc gactaaatag
    25981 gcatcggtgt tttttagctg atttctgatg agttctgggt gactgctagt ggtattgatg
    26041 agtctcataa atgtcctcct tagtattcct gtacctatta tagcactaat ttaaagcctt
    26101 agttagtaga atgatgcttt ttttgccatt ccttgactcc ccattgatgg ctctctcgtt
    26161 ttaatttagc aattgcctcg ataggagaaa accaacctaa gttattaaaa tcttctaagg
    26221 gcttagaaac ggcttgaaat gcagttactt cataaagata ggcaggatgg tagaagtggg
    26281 tatcacgatg gcgagagtag aaatattcat cagcctgacc gtaatatgaa ccaatagtag
    26341 cagaaaaacc aagttcttca atcagttcac gctctagggc ctgaagctga ccttcacctg
    26401 cttcaatttc accaccaggt aaaaaccagg aaccattagg agcttgaaca agaataattt
    26461 ttgtttgttc gtgattggga atgatagcat agacaccgta acgtgtgaca tagtctttat
    26521 gtgcattttt atgtccaaat gttggaatca tcattttatt tacctttcct ttttcttcat
    26581 tatactaaat ttttgctaat tattcttatc aatttgttta gtttaataaa aaattgatag
    26641 aaaaagagag agtctcctta tagaaactct cttagctaac gttaatcgag agactcttcg
    26701 ttaagaaaag aatctccctc tttaacagct ttaatcgtga tattttcact gtttgtcatc
    26761 agtgcacgta actgtttttc agttgggtgc tctaaataat agtctgtaat ggcatcttct
    26821 atataatctt ggatagtacg acggagcggc cgagcgccca ttttaggatc atagcctaaa
    26881 tctactaatt tttctttgac cttttgtgtc acatcaagat gaatcccatt atagcctaag
    26941 cgctcattaa catcttccag cattaaatca acgatatgca gcaaatgctc tttagataaa
    27001 gctttgaatt caataatacc atcgaaacga ttcataaact ctgggctgaa gaagttgctt
    27061 aattcaccaa ggacagaact tgtccgtcct tctctagcag caccaaatcc gacagaagct
    27121 tcgcttttac ctgttccggc attagaggtc atgataataa tggtgtcctt gaagctgact
    27181 gttcgtcctt ggccgtctgt taagcgaccg tcatcaagaa cttgtaagaa catgtgcatg
    27241 acgtcaggat gggctttttc cacctcatct aagagaataa gtgaatatgg attgcgacga
    27301 acttgttcgg ttagctgtcc agcttcttca tagccgacat aacctggagg agccccgact
    27361 aatttggcga cagcgtgttt ttccatgtat tcggacatgt caaagcgaat catattgttt
    27421 gtcgaaccaa agagttcaat ggctagttgt ttagacaatt cagttttacc aaccccagtc
    27481 ggtccaacga ataagaaaga accaataggg cgatttggag ttcctaatcc cacacggtta
    27541 cgacgaatag ccttagcgat tttatcaaca gcatcgtctt gcccgatcac gtgtgctttc
    27601 agatcattgg ctaagtttac gagttgagac tgttcctttt cttttaagtc cccaactgga
    27661 atattagttt tttgctctac aatagcttca atggtttttt cagtaatgat gggaatatct
    27721 tgctcatcga ctttttgagc ctgcatttct ttgtattttg taatttgatc gcggaaataa
    27781 gctgcgcgtt catagtcttc gtctcgagta gcttgcgcct tgagattctc agcttcaata
    27841 agacgtttgt caatttcttt aggatcaaca aagttgaggg ttagattcat tttggaacca
    27901 gcttcgtcca gaagatcaat agccttatca ggaagaaaac ggtcttgaat atagcggtta
    27961 gataaatgag cggcagcttc aatagcggct gggctatatt tgacatgatg atagtcttcg
    28021 tatttcggtt ggataccttt taaaatcgtg atggtttcct caacagaagg ttcatcaact
    28081 ttaacgggtt gcatacgtcg ttctaaggca gcgtcttttt caataatacg gtattcattt
    28141 aatgtagtag caccaacgag ttgcaactca ccacgggcca aggctggttt taaaatatta
    28201 ccagcatcca tattgccgtc tcctgcagaa ccagcaccga caatctcatg aatttcatca
    28261 ataaagagaa tcacatcctt gcgattgcga atttcttcca ttaatttttg catgcgctct
    28321 tcaaactgac cacggatacc tgttccctga acgaggctaa ccacatcaag acgaatcact
    28381 tgcttgcctt ggagtttttg aggaacagta ccatcaataa ttttttgagc caaaccttct
    28441 acaacagcag ttttaccaac tccaggctca ccaattagca caggattatt tttagtacgg
    28501 cggttgagga tctcgataac gcgtgtaatc tcttcgtcac gaccaataac aggatcaata
    28561 ttaccatttc ttgcaatatc tgtgacatta atcccaaact cttctaacaa gccctttgct
    28621 tgctgatttg atgtttgtgg ctgagcaggt cgttgtccgt tgtagttacc accatagcgt
    28681 cctccgccat ttccattttg ccctgcctga gtaggtgggg tatttggaag attaccaaaa
    28741 gctctaaaat tattcaaatc accaaaaaag tcatcaaaga aaggacttgt ggatctatct
    28801 tgtgctctat atcctggggt taggccattt aaaatagaat tggcaggatc ggatttcatg
    28861 atttgataac aattttgaca gagatcaacc tgtctttgtt ttccattaac acttgtataa
    28921 agatgaatag tagattcgtt taaattacaa ttttgacaca gcataatata cctccgaggg
    28981 tttatctaaa agtcaaagtc atttagtcaa aaatggtcaa actttatatt catattatag
    29041 caccacggct tgggaaatca agtaaaaagt ttatcgcttt agcgttagct tttagagagt
    29101 aacatgacag agtaacttta ttaagtttcc aatgagcaaa tgtttaaaat cattgaaaag
    29161 agtaaaaaaa gtaaaatatc aataaatata ttactttcct tgttagtttc ccttgttttg
    29221 tgttaaaata agagggataa aaaacgaata ggagaaacga aatggaatca catttggtga
    29281 gaatcatcaa tcgtttggag ttgatggtga cagatggcgg taatctcaag cgaaattttg
    29341 aacgcgatgg tgttgttgtt gcagaagttg ccttttcaaa tgatcctgaa aatggacctg
    29401 ttttcacatt gcgtgatgtt gaagcgcgtg agtcatattc ctttgatagc attgacttga
    29461 ttgcaatgga aatctacgat ttattatatt aagcttgaca tagtaaagag acttgcctga
    29521 ggcaggtttt tttattgtca aaaagtattg tattaatatt catcagatgg tataattatc
    29581 tttattgtaa tgaacctagg acttatgagg agatataaat ggatttgtca tttttgccca
    29641 aatactgggc ctactttaac tacggtgtac ttgtcaccat tatgatttca gtcagcgttg
    29701 tcttttttgg aacccttatt ggtgtcttgg taaccctgat taagcgtagt catgtgaagc
    29761 cgttgacctg ggtcgttaat ctttacgttt ggatctttcg gggaacacct atggtggttc
    29821 aaatcatgat tgcctttgct tggatgcatt ttaacaatat gcctactatt ggttttgggg
    29881 ttttagactt ggacttttca agactacttc ctggaattat tatcatttca ttgaatagcg
    29941 gtgcttatat ttcagaaatt gttagagcag gtattgaggc tgtaccaaaa gggcaattag
    30001 aagcagctta ttcactaggt attcgtcctc aaaatgccat gcgttatgtg attttgcctc
    30061 aggcctttaa aaatattttg ccagccttag gaaatgaatt tattaccatt attaaggata
    30121 gtgctctttt acaaaccatt ggagtgatgg aactttggaa tggtgcccaa tcggtggtaa
    30181 cggctactta ttctccaatt tcccctttac tggtggctgc tttttactac ttaatggtca
    30241 caacagtgat ggcacagtta ttggcagtct tagaacgtca catgtcgcaa ggaggtaatc
    30301 attgatgact gaggctatta tttcaattaa agacttgcac aagtattatg ggcacaatga
    30361 agtgcttaag ggaattgatt tagacattat gccaggagag gttgtggtga ttattggccc
    30421 ttctggttct ggaaaatcga ccttacttcg aactatgaat cttttggaag taccaactaa
    30481 gggacagatt aggtttgagg ggattgatat taccgataag aagaatgata ttttcagcat
    30541 gcgtgaaaaa atgggaatgg ttttccagca gtttaacctc tttcccaata tgactatttt
    30601 agaaaatatc actttatcgc caatcaaaac taagggaatg gctaaagcag aggctgacaa
    30661 aacagccttg agcttgttgg acaaagttgg attatcagaa aaagccaagg cttatcctgc
    30721 tagtctttct ggtgggcaac agcagcggat tgcgattgcg cgtggactgg ctatggatcc
    30781 agatgtttta ctctttgatg aaccaacttc agctctagac ccagaaatgg tgggtgaggt
    30841 cttggctgtc atgcaagatt tggctaaatc tgggatgact atggttattg tgactcatga
    30901 aatgggcttt gctaaagaag tcgcagatcg tgtgattttt atggatggcg gcgttattgt
    30961 ggaagaagga agtcctaacc aactttttga tctgactaaa gaagaacgta caaaagattt
    31021 tttgagtaga gtattataaa tctgttctat gaattataaa aggaagtcct aaatcattta
    31081 ggacttcctt ttataatttg ctaaggcatt ttataggaga ttacttttct ttttgttaaa
    31141 ataaaaagaa atagttgcat tatttgcata gaagaaaggg atctgttatg gtttcagtga
    31201 tgtggtttcg tagagattta aggattgatg atcaaaaagc tttagctcat gctattgcta
    31261 gtcaatcacc aaccttatgt gtgtttcaat ttaacaagga gcaattaaat ctgctacata
    31321 gtcggaatca atctagtttt attactagcg ttttggcttt tagaaagtta ttaaaatgtg
    31381 aaggtattga cttatatcta atgtatggag acttaatgac ctgttttgag caattattga
    31441 cacaattaaa agactggaca gatgtgtatt ttaactatga tgaatctgga tatggtcgtt
    31501 tacgtgatca aaaagcagca caatttttca aaaaaaatgg gattgctgtt catacctacc
    31561 aagatcacta tttacatggt agtcaagaga tcattaacca atcaggtcag ccttataaag
    31621 tttttacgcc ctactatagg atttggcaaa attatccgaa agagactcct attaaagtag
    31681 aactatcgca aggcaggtgg ttaaatcttg agacaccaga tgatgtttta aggactgttg
    31741 agagttttaa agatgaaaaa tatcaggatg ttgcaacttt tgatgaagcg tcaaaacaac
    31801 taaatcgatt tattcaagac caattagcag cttatcacgc taatcgagat tttccagcgc
    31861 agctagggac gagtcggttg tctccttttt taagaatagg agctattggc attcgcacgg
    31921 tatatcacgc tgtgcgtcaa gcaccaaata gtctagggca agctactttt ttaaaagaat
    31981 tagcttggag agacttttac aatatggtct atgtggctta tcctgatcag aaaactcagc
    32041 ctatccaaaa agccttttct caaatcgaat gggttaataa tcctgactgg tttcagttgt
    32101 ggaaagaagg taaaacgggt taccctattg ttgatgctgc catgcttcag ttacaaaaga
    32161 caggatggat gcataatcgt ttacgaatga tagtagcttc ttttttaact aaagatcttt
    32221 tatgtgattg gcgtttagga gagcagtatt ttcaacaaca actaattgat tatgatgctg
    32281 ccagtaatat cggaggatgg cagtgggctg cttccactgg aacagatgct gttccttatt
    32341 ttagaatatt taatcctgtt actcaaggta agcgttttga tcccaaaggc gagtttatta
    32401 aagcgtattt gcctcaacta gaacacgttc cagagaagta tctccacgaa ccgtggaaga
    32461 tgccaaaata ccttcaagag agtgtatcct gtattattgg cacagactat cctcaaccaa
    32521 ttgtcgatca tgctaaacaa cgtgaacaag ctattgcaaa gtatgagtgg gcaaaagaaa
    32581 aagcaaaaat agaatgactg ttctcattat aagagtttaa acaaaaagtt atcatgaatt
    32641 aagattattt aatttaaaaa ataagttgac tttatttaga ttatttgata ctattcagtt
    32701 gtctccctta gagaaagcga tttcttgttt tggagctgat ttttaccatt tggtcttaga
    32761 ataggatgag taatatgact tacaacgagg tatatcaaga atggttgcac aataatgatc
    32821 ttagtgatga tattaaagca gatttagcag ccataaaaga caatgaggct gagattcaag
    32881 atcgttttta caaaacactt gaatttggaa cagcagggct aagaggaaaa cttggagcag
    32941 gcaccaatcg catgaatacc tatatggttg gtaaggcagc acaagcttta gctaatacta
    33001 ttattgatca tggacctgaa gcggttaaaa agggcattgc ggttagttat gatgttcgct
    33061 atcaatctag aacatttgca gaattaacat gctctattat ggcagctaac ggtattaagg
    33121 cgtaccttta taaagggatt cggccaacac caatgtgttc gtacgctatt cgtgctttgg
    33181 gatgtatctc tggtgttatg attacagcta gtcacaatcc ccaagcttac aatggttata
    33241 aagcttattg gcaagaaggt tctcaaattt tggatgacat tgcggatcaa attgcacaac
    33301 acatggctgc actaactcag tatgaggaaa tcaaacaaat gccttttgaa aaggctctgg
    33361 acagtggact tgttacttat attgatgaga gtattgaaga agcatataaa aaagaagtgc
    33421 ttggtttaac gattaatgat actgacattg ataagtcagt ccgagtagtc tatacccctt
    33481 taaatggtgt tgggaatcta ccagtgcgtg aggttttaag acgccgcggt tttgaaaatg
    33541 tttatgtggt acctgagcag gaaatgcccg atcctgattt tacaacagtt ggctatccta
    33601 atcccgaagt ccctaaaact tttgcatatt cagaaaaatt aggtaaggca gttgatgctg
    33661 atattttgat tgcaactgat ccagattgtg accgtgtggc tttagaagtt aaaaatgcag
    33721 tcggtgacta tgtttttctc aatggcaata aaatcggagc gcttttatct tactatatct
    33781 tttcgcaacg atttgactta ggcaatttac cggctaatcc tgttttagtg aaatccattg
    33841 taacaggaga cttgtcacgg gctattgcta gccattatgg tattgaaacc gttgaaacat
    33901 tgactggttt taagaatatt tgtggcaagg caaacgaata tgatgtgacg aaacagaaaa
    33961 actacctgtt tggttatgaa gaaagtattg gtttttgcta tggcactttt gttcgtgaca
    34021 aagacgctgt tagcgcctct atgatgattg tagaaatggc agcttactac aagaaaaagg
    34081 ggcagaattt actagatgtt ttgcagacta tttatgcgac atttgggtat tataatgaac
    34141 gtcaaattgc cttggagctt gaggggatag aaggacaaaa acgcattgcg aggataatgg
    34201 aagatttcag acagacacca atagctagtg ttgcagagat ggcgttagac aagacaattg
    34261 attttattga tggctatcaa gattttccaa agcaaaactg tctcaaattt tatttggatg
    34321 atggttcttg gtatgcactt agaccatcag gaacagagcc taaaataaaa ttttatttat
    34381 atactattgg ccaaacacaa gaaaatagtg caacaaaatt agatgctatt gaagcagcgt
    34441 gtcgcacaaa aataaatcag gttaattaaa catcaaaaat ggataacact tttacactat
    34501 tttccatata aacaactaca aaaggaggtg agacttttag gcaccttctt tttcttgtag
    34561 gtgccactaa tcaatttaat cgactcaaac caatactaaa aaataagtaa gagtataaag
    34621 aacaggcatc tatttttttg ttataataga aaaatgaaat taaagggaga tgtcaatgac
    34681 agaactaatt gatggtaaag ccttagctca aaagatgcaa caagagttag cagctaaagt
    34741 caacaatcta aaacaaaaaa aaggaattgt accaggctta gccgttattc ttgtaggtga
    34801 tgatcctgct agtcaggtgt atgtccgtaa taaagagcgt gcagctctta ctgtaggttt
    34861 taaaagtgag accgttagat tatcagaatt catttgtcaa gaagagctta ttgcagtaat
    34921 cgaacgttac aatgcagata acactattca tggtatttta gtgcagctac ccctgccaaa
    34981 tcatattaat gataaaaaaa ttattctcgc cattgatccc aaaaaagatg tggatggttt
    35041 tcacccgatg aatacaggtc acctttggtc aggacgtccc ttaatggttc cttgtactcc
    35101 atcagggatt atggaattgc ttcgagaata taatgttaac cttgaaggta aacatgccgt
    35161 cattattggc agatcgaata tcgttggaaa accaatggca cagctcttac tagacaaaaa
    35221 tgcaacagtc acgttgacac attcaagaac acgtcaatta gaagaagtat gtcgctgtgc
    35281 agatgtgttg attgtggcaa tcggacaagg tcatttcata acaaaacaat atataaaaga
    35341 tggtgcgata gtgattgatg tgggaatgaa ccgtgatgat aatggcaagc taattggaga
    35401 tgtggccttg gatgaggtgg cagaagttgc agcgaaaatc acccctgtac caggaggtgt
    35461 cggtcctatg acgattgcta tgttgctaga gcaaacttat caatctgctc tccgtagtac
    35521 tcataaatag ttatcattag acaattcaac ataagagaat ataagaagtc agcaatggct
    35581 tccttttttg tttttctgct cactatttat ccagaaaaat ggtataattg cattagtatg
    35641 caaagggact atgtcaggtc caactaagtg aaaggtggct tgatggcaga ttatttaacc
    35701 gtcactcatt tgacgaaata tttaaaatta aaatttgacc gtgaccctta tttggaacgg
    35761 gtttatctga ctggtcaagt gtccaatttt cgaaaacgac caactcatca atatttttcc
    35821 ttaaaagatg aaagtgctgt gattcaagcc accatgtggg caggagtcta taaaaaacta
    35881 ggatttgacc tagaagaagg catgaaaatt aatgtgattg ggcgagtcca actttatgaa
    35941 ccgagtggtt cctattctat cgtgattgaa aaggcagagc cagacggcat aggtgccttg
    36001 gctttgcagt ttgaacaatt aaagaaaaaa ttaactgcag aaggttattt tgagcaaaaa
    36061 cacaagcagc tcttaccaca gtttgtgtca aaaattgggg tcattacaag tccaagtggc
    36121 gctgtgattc gagatattat cacaactgtt tcacggcgtt ttccaggggt tgagatttta
    36181 ttattcccaa ctaaagtaca aggtgatgga gccgcccaag aagtagtggc gaatattcga
    36241 agagccaacc aaagagagga tttggacttg ctcattgttg gccgtggagg tggctcgata
    36301 gaagaccttt gggctttcaa cgaggaaata gtagttcagg ctatctttga atcgcaactt
    36361 ccagtgattt cgagtgtggg tcatgaaact gatacaaccc tggcagattt tgtggcagat
    36421 cgcagagcag ccacaccaac agcagcagca gagttagcaa cgcctattac aaaaacagat
    36481 cttatgtctt ggatagtaga gcggcaaaat cgttcttatc aggcctgctt gaggcggatt
    36541 aagcaacggc aagagtgggt ggataaacta tcacaatctg ttatttttag gcaaccagaa
    36601 cgattatatg atgcctacct gcaaaaaatt gatcgcctta gcatgaccct gatgaatacg
    36661 atgaaagatc gtctgagctc cgccaaagaa aacaaggttc agttggacca tgccttggcc
    36721 aatagtcaat tacagacaaa aatcgagcga taccaagacc gtgttgctac cgctaagcgt
    36781 ttactgatgg ctaatatggc tagtcaatat gatagtcagc tggctcgctt tgaaaaagca
    36841 caggatgctt tgctatcact ggatgccagc cgaattatcg cccgtggtta tgcaatgatt
    36901 gaaaaaaatc aagcactggt agcctctgta agtcagataa caaagggtga tcagttaacc
    36961 attaagatgc gtgatggaca attagatgta gaggtaaaag atgtcaaaaa cgaaaacatt
    37021 tgaagaaaat ttacaagatt tagagactat tgtgaacaaa cttgaaaatg gggatgtccc
    37081 tttggaagaa gctatttcag aatttcaaaa aggaatgctt ctctcaaaag aacttcaaaa
    37141 gaccttacaa gcggcagaaa aaacactcgt caaagtgatg caagctgatg gcacagaagt
    37201 agatatggat gattaatgga caaattagct agaattgacg aagccattcg tcgctactat
    37261 aagacgacta gtaacggtgt atctgaagag ttgattgatg ctatcttgta ttctgttgac
    37321 agtggtggca agcgcattcg accacttatc ttattagaga tgattgaggg atttggggta
    37381 tcgttacaga atgctcattt tgatttggct gcagcccttg aaatgattca tacaggaagt
    37441 ctgattcacg atgatttgcc agccatggat aatgatgatt accgacgtgg tagactgacc
    37501 aatcacaaac aatttggaga agccacggcc attttggcgg gagatagctt atttttagac
    37561 ccctttgggt taatagctca agctgaatta aatagcgagg ttaaagtagc cttaatccaa
    37621 gaactttccc tagcttcagg aacttttggc atggttggtg gtcagatgct agatatgaaa
    37681 ggggaaaatc aagctttgag tctccctcag ttgtcactga ttcacctcaa taaaaccgga
    37741 aaattattaa cttttccttt taaagcagca gctcttgtaa cagaacaggc tattactgtt
    37801 cgtcaacaac tagagcaggc gggaatgctc atcgggcacg cctttcagat tagggatgat
    37861 attttagacg tgacagctag ctttgaagat cttggaaaaa cgcctaaaaa ggatttattt
    37921 gcagaaaaag ctacttaccc aagtttactg gggttagaag cctcctacca actgttgaca
    37981 gagagtttag atcaggcttt gacgattttt cagacactag aaaacgatgt aggctttaag
    38041 cctcaaataa ttacaaaact gatagaaggg ttacgactta atgcctaaag aaagagtaga
    38101 tgtgttagcc tataagcaag gattatttga gaccagagag caagctaagc gtggtgttat
    38161 ggcaggccta gtggtctctg tgattaatgg ccaacgctat gacaagccag gtgacaaaat
    38221 tgacgatggg actgagttaa aacttaaagg tgaaaaactc aaatacgtca gtcgtggtgg
    38281 attaaaactg gaaaaggggc tgcacgtttt tggtgtatcg gttgctaatc aaattgggat
    38341 tgacattggc gcttcaacag gtggttttac cgatgttatg ttacaagatg gggccaaact
    38401 agtctatgcc gtcgacgttg ggactaacca gttggtatgg aaactcagac aagatccacg
    38461 agtaagaagt atggaacagt ataactttcg ttatgctcag ccagaggact ttagtgaggg
    38521 acagcctgta tttgccagta ttgatgtgtc ctttatttct ctcagcttga ttttgccagc
    38581 cctgcataac gttttgtcag atcagggaca ggttattgct ctcattaaac cgcaatttga
    38641 agctgggcgc gagcagattg gtaaaaaagg catcgtcaaa gacaaacaga ttcatgaaaa
    38701 agtgattcaa aaggtcatgg attttgcctc aggttacgga tttacagtta agggacttga
    38761 tttttcccct attcagggtg gtcatggcaa tatcgaattt ttagctcatc ttgctaagtc
    38821 acagacacct gaaacgttag ccccgcattt gattcagaaa gttgttgcca aggcacataa
    38881 ggagtttgag aaacatgaaa aagagtgaac gacttgaatt aatcaaaaaa atggttttga
    38941 cgcatccaat tgagacgcaa catgacctct taagattatt ggcagagcat gggttagaat
    39001 taacccaggc tactatttca agagacatga atgaaattgg tatagtaaag attccttctg
    39061 gcagtggacg ttacatctat ggtctttctc aagatagtgg aaaaaagatc gttcaaggac
    39121 ctagatcgat aaagagcact attttagctg tatctgacaa aacaaaaggt ttagaacaac
    39181 acctctattt aaaagtcgta cctggcaata gtaaattaat caaacgttat ttattagcag
    39241 atttttcaaa agctattttt agtcttattg ctgatgatga tagtttattg ttaattgcaa
    39301 agtctccttc agaagcagat atgatacgcc aagaaatttt gctttggatg cagggtataa
    39361 cctagaaaaa aataggggag aaagtgatgt tattagaaat ttctattaaa aattttgcta
    39421 ttattgatga aatttcccta aattttgaaa atggtatgac ggttttgacg ggtgaaactg
    39481 gagcaggaaa gtctattatc attgatgcta tgaatatgat gcttggtgcg cgtgctagta
    39541 cagaagtgat tcgtcgtgga gctaataaag cagaaattga ggggttcttt tcggtagatg
    39601 ctaccccaga actggttgcg tgtctggaat catcaggtat tgctatggaa gaagaactga
    39661 ttattcgccg agatatcttt gccaatggca gaagcgtgag ccgcattaat ggtcagatgg
    39721 ttaacctagc caccttaaaa caggtcggac agtttttggt agatattcat gggcaacatg
    39781 accaagaaga attaatgcga ccacagctcc accagcaaat tttggacgct tttggtgata
    39841 aggcttttga gcaattgaaa gagaactatc agcttatttt tgatcgttat aaaagcttgc
    39901 gtcgccaggt tattgacaaa caaaaaaatg aaaaagaaca taaagatcgt atcgacatgt
    39961 tggcttttca gatagcggag attgaagctg ctgccttgag tcgaggtgag gacgaccggt
    40021 taaatcaaga acgtgatcgc ttaatgaacc acaaacaaat tgctgatacc ctgaccaatg
    40081 cctacgtcat gctagataat gacgattttt caagtctatc caatattcgc tctagcatga
    40141 atgacttact atcaattgag cagtttgatt cagagtacaa agggatgtcg acttcgattt
    40201 ctgaagccta ttatattctt gaagaagtga gcaagcaatt atcagatacc attgaccaac
    40261 tggattttga tggtgggcga ttacaggaaa ttgaatttcg cttggataca ctcaatagct
    40321 taacccgtaa atacggtggt aatgtgaatg atgtgcttga ctactacgat aatattatca
    40381 aagaatacca gctattaaca ggagatgact tgtcttcagg ggatctagag gcagaattga
    40441 aaagcttgga aaaacaattg gttgctgctg cttcggagtt aagtgtctct cgccatcaat
    40501 tggcggagca attagaagct gagattaaag ctgaacttaa ggaactttat atggaaaaag
    40561 cagatttcaa agttcacttt accacatcaa aattcaatcg tgatggtaat gaaagcttgg
    40621 aattttacat ctctactaat ccaggcgaag gattcaagcc ccttgtcaag gtggcttctg
    40681 gaggagagtt atcacgcctt atgttagcca tcaaggctgc tatttctaga aaagaagata
    40741 agacgagtat tgtttttgat gaagtagata caggtgtttc tggtcgcgtt gcgcaagcca
    40801 ttgcccaaaa aatttataaa attgggcgac atgggcaagt tctcgctatt tcacacttac
    40861 ctcaagttat tgctattgct gattatcagt attttatttc taaagaaagt aaagaagagt
    40921 ccaccgtttc taaggtaagg ttattaactc ctgaagaacg tgtggaagaa attgctagca
    40981 tgattgcagg aacagatatg acacaagctg ctcttacgca ggctcgtgaa ctacttgcca
    41041 aacattaaga ttatgtttgg tatttgatat tttcataatt agatgacatt gattttttag
    41101 gtgctaaaat aatcattgtg tcagaccatt tttcctctgt taatctggct tggtttgcct
    41161 tgacaaacct ctgagaaata gtagaataga taagagaatt ttaagctgag gggatagaaa
    41221 tgactagtaa aaaagcgtgt ttatcaagca tcattgtgtt agcaagttta acgtgtggaa
    41281 atgatactgt tagtgccaat catctctcag caactggaga taagtttgat gattgctcaa
    41341 cacttgttga aaaagatgtg gcccctaaag atgaacttga gatgttagca tggtcctcgt
    41401 ctcaaacaac tgatgatgct gacagagact atgaagattt tctcgatgat gattctttta
    41461 atgatgattc ttttatttct caaaatgaaa ctgataagat gtttgagaat ttaactgatg
    41521 ataggttatt agatgaatta gatgaagaag atacaattga gccagagcaa aatgtaataa
    41581 tgcctagtga cgatgagcta tttgatttaa ctgatgctgt tgagacacgc cttactgttt
    41641 ctagtgctcc ccatttagag gctgaattgc cgaaaccaca tttgaggagc ctatcagata
    41701 cagcactgcg gtctggtgaa attagaggac atttagataa caaactggac gctttgtctg
    41761 taacagctac aaagttagca ttaacgatgg ctcaaaaatt tgatttgaca acgcatgtct
    41821 attctatagg tgaaagcttt agtgaagtat tagctgctca ttatgaagac agaaaagcag
    41881 aatcagcttt ttctaagaaa aagagatttc accttcctat tgctactcca gatgttgtta
    41941 tagaggagtt aaggcgcctg gtctcttcta ttggaagttc aaaagaagat gtttcagttc
    42001 cttataatcg gaagctaggt atggcagttg caaaaagaaa aatagccctg ccacaaacgg
    42061 gagaggagtt ctcttattat ccagctttac ttggtttaat gatattagga ttaacgccga
    42121 ttatgatacc aaagaagata aataattagg taataagtta ttgtagttgt aaaagacggg
    42181 gcaaatgttc aagcgtgaaa aggttgactt ttgcctcgtt ttgtatctcg ttattgctaa
    42241 ggcattttct tcgagcagct agtgctcgcc atacctatct accgagtgac tattcaatag
    42301 cactaccagt tttgctttat ttagcaagga gtgttagaat aaagaggtga gaaactagtt
    42361 tagaatggaa aaatacatgg gaactataaa aattgttaca gattcatcaa taactattga
    42421 accagaatta attaaagctt tagatattac tgtagtgcct ttatctgtca tgattgatag
    42481 caaattatat tctgataatg atttaaaaga agaaggtcat tttttgagtc ttatgaaggc
    42541 tagcaaatcc cttcccaaaa ccagtcagcc accggttggt ctctttgctg aaacttacga
    42601 aaatttggtc aaaaaaggag taactgacat tgttgctatt cacctctcac cagctttatc
    42661 aggtactatt gaagcttccc gtcagggggc tgaaatcgct gaggcccctg ttacagtttt
    42721 agattcagga tttactgatc aagccatgaa atttcaggtc gttgaggcag caaagatggc
    42781 aaaagcgggt gctagtttga atgagatttt gactgctgtt caagctatca aatcaaaaac
    42841 agagctctac attggtgtat ctaccttaga aaatttagta aaaggaggtc gtattggtcg
    42901 tgtgacgggt gttttgagct cgctacttaa tgtcaaagtt gttatggcac ttaaaaatga
    42961 tgagttaaaa acgcttgtca aaggtcgagg taataaaaca tttacgaaat ggttagatag
    43021 ttacctcgct aaaaatagtc atcgcccgat tgcagagata gctatttctt atgctggtga
    43081 agctagcctg gcattaacct taaaagaaag aatcgcagct tattacaacc actctatttc
    43141 ggttttagaa acaggctcta tcatccaaac acatacagga gaaggagctt ttgcggttat
    43201 ggttcgttat gaataatcgt catttattta gtgggatatt tttctttgtt attagtttat
    43261 gtctggcctt tttattgcta aatattatta tccctaagtc aaattcacgt ttgaaaaaga
    43321 gtgattttct gaaaaaagaa caagtagcta tccaatatgt tgctatagga gattcattga
    43381 cagaaggagt aggtgatcta actcatcaag gtggttttgt tcctttgtta acgaatgatc
    43441 tcagtgaata ttttaaggct aatgttaatc atcaaaatta cggcgtatct ggtgatacca
    43501 gtcaacagat tcttgatagg atgataaaac aaaagcagat acagttatct ttaaaaaaag
    43561 cagatataat gacgttaacc gttggtggta atgatgttat ggcagttatt cggaagaatt
    43621 tagcggattt gcaagtttct agttttagaa agccagctcg tcagtatcaa aaacgattaa
    43681 gacagattat cgagttagcc agaaaagata ataaagatct tcctattttt attttaggca
    43741 tctataatcc gttttatttg aattttccag aactaactga tatgcaaaaa gtgattgatg
    43801 actggaatat caaaactaag gaggttgttg gagaatacga tcgtgtgtac tttgtgccaa
    43861 taaatgacct cttgtataaa gggataaatg gacaagaagg aattgtccat tcttcaggag
    43921 atcaaactac aattgtcaat gatgccttgt ttactgggga ccattttcac ccaaataata
    43981 ctggctatca aatcatgtca aatgcagtaa tggagaaaat taaaaagcat gaaaaaaaaa
    44041 tcaaacctta attggtggaa gtggagcttc ttatgtctgc ttgcttttaa taccgccttt
    44101 ttgatggtta ttgcaagtcg tctcattcaa gtaagagaac ctgagtcaga actgattgct
    44161 aaaaaaccag ttaaaaatat caaaattggt acatttgtca caaccagaga acagcttaac
    44221 gaaactgtag caagttatct taaagattat cagactaaaa aaatgtctta taaattttat
    44281 gcgacatctt cttctatttt gtttgaagga acttatcaat tattagggta tgaagtccct
    44341 ttatatatct attttcaacc tcatcgtcta gaaaatggag ctgtccaact acaggtgatc
    44401 tctttttcag tgggaactct gccactccca gaaaaagatg ttttgcagta tttgaaatca
    44461 agctacaaat tgccaagctt tgttaaagtg atgccaaatc aatcagctat cgttgttaat
    44521 ttacaagata tccaaaatga tgctaaggtt tatttaaaag ccaagaaaat tgatttattt
    44581 aatgatgaaa tcagttttaa catctataag aaataagctc aaataacgtt aaatacttga
    44641 cctaatcgct ttttttagat atgataatat ctgttaaagc attaagctta ctaattattt
    44701 ggaggatttg ttaaatggct aacaaacaag atttaatcgc aaaggttgca gaagcaactg
    44761 aattgactaa aaaagattca gcagcagcag ttgatgccgt gttttctaca atcgaagctt
    44821 tccttgctga aggtgaaaaa gtacaattga tcggttttgg taacttcgaa gtacgcgaac
    44881 gtgcagctcg taaaggtcgt aacccacaaa ctggtgcaga aattgaaatt gcagcttcaa
    44941 aagttccagc cttcaaagct ggtaaagctc ttaaagacgc tgttaaataa ttgcatatat
    45001 aaaagcccat tgtacaagcg ttgtagcctg tgcagtgggc ttttttgtgt tcaggggggt
    45061 aaaaaagggg taaaaaattt aatagttttc cataatatca gctacacttg atttcatttt
    45121 ctttgtgaca tgagtataaa tttgggcagt agttttagcg tctgcatgtc ctacacgttc
    45181 catgattgat tttagcggca cgttattctc tgctagacgg ctaataagag tgtgacggaa
    45241 gatgtggcta gtaagttttt ttgtaattgg gtcttccaat ctttcattag ctttttttaa
    45301 agctaagttg aatgagttag tctgaattgg aacaccgttt tttgttgtaa aaaggtatcc
    45361 catatctcta tatctatgat ttgtattttt ttctagttcg ttcataaatt ctaactcttg
    45421 tagaatttca agttctctac ttgtcatgat ggtttcacga taagaagcta atgtctttgg
    45481 tgctgtcttt tccccgttac gatatccttc tgtatgatca aatgttccgt gcaactgcaa
    45541 tattcttgac ttagattcgt agttgcacgg ctcaatgctg acagcttcac cgatacgaca
    45601 accgttcaaa ctgataaact cagccaacaa accaagtcgg taagtgctag gtcttctgta
    45661 caattctttc aataatggtt taatttcatc ttcttcaaga tatttttctt cagccttttt
    45721 ccagtcttca agagttttct ttacccttgg gagtttagca cgtcttgcag gattatcttg
    45781 gataatatcc aaaccaacag cataatcaaa tgctaagttt agcatagact tattacgctc
    45841 ttttttattc ctagagcaat ctaggttatc gagataattc tgaacgtatt taggatcaat
    45901 attcactact ttaacaccta ttccaaaagt ttcccttatc tcttcgatat tacctctcag
    45961 agaagctaca gacgaccttt taagttcttt cttataaaac gcccaccatt catctaaaag
    46021 ttccgtgaaa agtaactcag aggcctttaa ttgcgctatt ttttctgtta ttttttgttc
    46081 aaggattttt cgagctgctt tataagctct tgaattatcc ttctccatag taacggatac
    46141 aggtttccat gttccagtgt aaggatcttt ataattttca cgaaatttga atttaccgtt
    46201 atcagtttct tctatccaca tttgtttttt acctcatttt ctgttaaaat aggtatagta
    46261 aagagaccta ctgcgaagca ggtttttact atactagatt cgcctcacgc tctcctcgcc
    46321 aaatttgagc gtggggcttt tttgttagtt gagaaaccaa gatgctatcg actctaataa
    46381 tccagcattt ttactttttg atactctatc tacttttaaa atagtcaatc gtaacttata
    46441 agttatatct ttctttttag taaccacggt ctttaaatcg tttttgagtg ttttgtattg
    46501 tccaccacga ccagtaaggg ttgcttccat ttggtaatga ctagattttt ttacataagg
    46561 ggttaatcgt ttagctaagt ctgctggaaa gtagccaaca aaagttttgt ttaccatgac
    46621 ttttatggca tttggatcgt gacgattaag aggttctctg acaatctcta catcttttgt
    46681 tgagaccctg taatacttgt agatatcctt gaagtttgat tttatgtagt cagaaataaa
    46741 atctttatcc ttatcccagt atggtgattt cttaccaatt tcagccataa ctttattagc
    46801 ttctttttca tgataatttg ttcccattaa gagaaagtta tcttggaata tgacttttgc
    46861 cataacagtc tcctttaata ttccctataa atatccacaa cttcaccgat tgtgcggatg
    46921 tcgtcatcct ttgatagata gatttcctca tagcttttat ttaaactttg caagtaccaa
    46981 gcgccatcat aatcacgctt gagttttttt acaaagtttt taccattgac ttggaatatc
    47041 cctatatcat tcatatcgac ttggctagat accttgatga atagcaaatc gttgtcttga
    47101 ataagtggtt ccattgaatc gccagctact tttgcaatag tatcatactc atcggggaca
    47161 tcatcagaac gtagtttaac tttcatgtgt aaattgtctt cttggtatgc cccaagacca
    47221 gcggcaacca atccctcaac gtagtcagtg atataatctt ctgtctcctc agatttttta
    47281 tcgaatatag aaacggtttt attttgttct tctaattgtt cgttagcgaa gttgaggact
    47341 ttttcttgtc taggttgttc tagttggttg tatatagaaa cgatttcagg ttgttcgttc
    47401 tgtattgtga ttttcccaag tttactatct gtgagtccta aaagatagtc agaggtaacg
    47461 ttgaaaatct ctgctagttt ttttaggtct ttaccttttg gaaaattttc attcttctcc
    47521 cactttgaaa cagtagtata ggttttcatg cccaatattt ctgaaagttc agtctgagtc
    47581 atgttgcgcc cttctctcaa ctttcttatt cggtccccta attgctccat aggatacctc
    47641 ctttttaatt ttgataagta aattatatca aaaccatgat tataaatcaa ccatataaga
    47701 aaaaagtttt aaataaacta tatgatttac gattttttca aaaaaacaga aaataaatca
    47761 tataaaacac ttgacgcaag atttaaaatc atatagaatg taatcatcat caagaaagga
    47821 gcgactcagc tatggtaact attgcagaat taagagctaa acataataag atgtcacagc
    47881 gtgaattggc aaaagaatta ggtgttactc aaacatctat cagcaactgg gaaaaagacc
    47941 agacaaagat ttcaggtgag tatcttatta gtctagcttt gtttttcgat gtatctaccg
    48001 atgacattct tgggataaac aagcaagcaa cacaaaaatt ttaaacacta tatgatttta
    48061 aatcatatag tagaaaggac acacatgaac gaaataacat ttaccctata ttgcacaaca
    48121 tctgaagaag caatcacgga agtaaagaaa ctaaaagaag cccatccaaa ggatagactt
    48181 cgattcaatg taaacattaa gcctgagttt tactaataga accaacttaa ttagataata
    48241 aaaagtccga cgggaatcgg actccaaaca aaaaatattt acttaattat aacacaagag
    48301 agaggatatt aatatgccaa aagcagaaat tacttataga gcagttgatg ttaatgaaac
    48361 agcttctcat ggtgattata aacacttgat tcaacaatgg gaggggttga cagttgctac
    48421 tgcaaagcag tgggcgacag aaatgcgtga tcatccagac tttaaacagt ttgtattaaa
    48481 tccaacgcat agaatcgtat tcattgatta caaaggattc aaactgtttg tacagtggaa
    48541 atctcgcaat cgttacaaaa caaaaaaaga gacactgcca gaaatgcttg aaaatattaa
    48601 atttgaaaaa agagtaggag tataacatga acaaattaga attatttttg ttagcaacaa
    48661 ccgttatctt agcggtcatt gctagggtgc aacacgaagt cattaaaaaa cacaattcgc
    48721 cagagaataa gcggaaaatt tttagggaag tagctttaaa aaacagcaaa ggatggagca
    48781 agaagcgtgc aagaggaggg gttgtcagct aatgcagtac atttttcaag acattaacag
    48841 gtagttacac agctgttagc aatgaattta ttcaggacaa agaacttttc aataaggaaa
    48901 aaggactgct gctaaccatt ttgagtaatg cagacgaatg gagagtctat cctgaagaac
    48961 tcgctagacg ttgtaaggat agcgaatctg caatcagaag ccagttgaaa gcgttagaga
    49021 atgccaagta tatcagaact tatagaaaat catttggcgg gcgatatggt accgaagcct
    49081 atagattctg ttctgacaga aaaataagcg acgaggcgtt caatactttg aaggcagaac
    49141 aggacttaga actagaaaaa attgctaata cctaatttgc taatgtgcaa tttgctaatg
    49201 tgcaatttgc taatcaacaa attagccaac taat
//
LOCUS       DAUCWO010000006        46836 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUCWO010000006 DAUCWO010000000
VERSION     DAUCWO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46836
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.006"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            161..1393
                     /locus_tag="VGY20_000323"
     CDS             161..1393
                     /locus_tag="VGY20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4273211.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1561..2742
                     /locus_tag="VGY20_000324"
     CDS             1561..2742
                     /locus_tag="VGY20_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4273212.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGIKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2894..4312)
                     /gene="sufB"
                     /locus_tag="VGY20_000325"
     CDS             complement(2894..4312)
                     /gene="sufB"
                     /locus_tag="VGY20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ4273213.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4305..4784)
                     /locus_tag="VGY20_000326"
     CDS             complement(4305..4784)
                     /locus_tag="VGY20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ4273214.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4771..5997)
                     /locus_tag="VGY20_000327"
     CDS             complement(4771..5997)
                     /locus_tag="VGY20_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4273215.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6028..7290)
                     /gene="sufD"
                     /locus_tag="VGY20_000328"
     CDS             complement(6028..7290)
                     /gene="sufD"
                     /locus_tag="VGY20_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ4273216.1"
                     /translation="MTKEKLVAFSQVHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGIVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLNQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7385..8155)
                     /gene="sufC"
                     /locus_tag="VGY20_000329"
     CDS             complement(7385..8155)
                     /gene="sufC"
                     /locus_tag="VGY20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ4273217.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8277..9446)
                     /locus_tag="VGY20_000330"
     CDS             complement(8277..9446)
                     /locus_tag="VGY20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ4273218.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLIAAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9453..10214)
                     /gene="mecA"
                     /locus_tag="VGY20_000331"
     CDS             complement(9453..10214)
                     /gene="mecA"
                     /locus_tag="VGY20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ4273219.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAVDPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10360..11199)
                     /locus_tag="VGY20_000332"
     CDS             complement(10360..11199)
                     /locus_tag="VGY20_000332"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ4273220.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKQNAQQEPAVTDLARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTHYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11265..13190)
                     /locus_tag="VGY20_000333"
     CDS             complement(11265..13190)
                     /locus_tag="VGY20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ4273221.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKILEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLAQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13388..14956
                     /locus_tag="VGY20_000334"
     CDS             13388..14956
                     /locus_tag="VGY20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ4273222.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEAKSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14949..15689
                     /locus_tag="VGY20_000335"
     CDS             14949..15689
                     /locus_tag="VGY20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273223.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15831..15947
                     /locus_tag="VGY20_000336"
     CDS             15831..15947
                     /locus_tag="VGY20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ4273224.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16173..17183)
                     /gene="gap"
                     /locus_tag="VGY20_000337"
     CDS             complement(16173..17183)
                     /gene="gap"
                     /locus_tag="VGY20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ4273225.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17531..19609)
                     /gene="fusA"
                     /locus_tag="VGY20_000338"
     CDS             complement(17531..19609)
                     /gene="fusA"
                     /locus_tag="VGY20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ4273226.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20093..20563)
                     /gene="rpsG"
                     /locus_tag="VGY20_000339"
     CDS             complement(20093..20563)
                     /gene="rpsG"
                     /locus_tag="VGY20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ4273227.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20584..20997)
                     /gene="rpsL"
                     /locus_tag="VGY20_000340"
     CDS             complement(20584..20997)
                     /gene="rpsL"
                     /locus_tag="VGY20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ4273228.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21205..23826)
                     /locus_tag="VGY20_000341"
     CDS             complement(21205..23826)
                     /locus_tag="VGY20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ4273229.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASEET
                     KASNTHDDSLPKPETIQEAKATIEAVEKTLSQQKTKLTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            complement(23817..24656)
                     /gene="purR"
                     /locus_tag="VGY20_000342"
     CDS             complement(23817..24656)
                     /gene="purR"
                     /locus_tag="VGY20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ4273230.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24923..25861)
                     /locus_tag="VGY20_000343"
     CDS             complement(24923..25861)
                     /locus_tag="VGY20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ4273231.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25851..27122)
                     /locus_tag="VGY20_000344"
     CDS             complement(25851..27122)
                     /locus_tag="VGY20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ4273232.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            complement(27124..27756)
                     /locus_tag="VGY20_000345"
     CDS             complement(27124..27756)
                     /locus_tag="VGY20_000345"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ4273233.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELLITANPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27749..28411)
                     /locus_tag="VGY20_000346"
     CDS             complement(27749..28411)
                     /locus_tag="VGY20_000346"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ4273234.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28421..29293)
                     /gene="rsgA"
                     /locus_tag="VGY20_000347"
     CDS             complement(28421..29293)
                     /gene="rsgA"
                     /locus_tag="VGY20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ4273235.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYNFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALEMGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29717..30589)
                     /gene="rsmA"
                     /locus_tag="VGY20_000348"
     CDS             complement(29717..30589)
                     /gene="rsmA"
                     /locus_tag="VGY20_000348"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ4273236.1"
                     /translation="MRIADYSVTKTVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30716..31306)
                     /gene="rnmV"
                     /locus_tag="VGY20_000349"
     CDS             complement(30716..31306)
                     /gene="rnmV"
                     /locus_tag="VGY20_000349"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ4273237.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEATPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMEAYEE"
     gene            complement(31278..32069)
                     /locus_tag="VGY20_000350"
     CDS             complement(31278..32069)
                     /locus_tag="VGY20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ4273238.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEIIKAAGVGPRGGIM
                     HSYSGSLEMARRFVDLGMMISFSGVVTFKKALDIQEAAQHLPLDKIVVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKATTANAKRVFKLD"
     gene            32354..33184
                     /locus_tag="VGY20_000351"
     CDS             32354..33184
                     /locus_tag="VGY20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ4273239.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNDNCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            complement(33295..34233)
                     /locus_tag="VGY20_000352"
     CDS             complement(33295..34233)
                     /locus_tag="VGY20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4273240.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKNEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITPEKIENNLASS"
     gene            complement(34255..35169)
                     /locus_tag="VGY20_000353"
     CDS             complement(34255..35169)
                     /locus_tag="VGY20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ4273241.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTFQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35181..35843)
                     /locus_tag="VGY20_000354"
     CDS             complement(35181..35843)
                     /locus_tag="VGY20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ4273242.1"
                     /translation="MTSKKQGLLHSLFNLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRTFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35887..36342)
                     /locus_tag="VGY20_000355"
     CDS             complement(35887..36342)
                     /locus_tag="VGY20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEQ4273243.1"
                     /translation="MIYDRLKNISRYRGIHPHLDAAITFLQTTDLRQLAEGKYPILGE
                     KVFAVIQRNQLSKADNSLLEYHKRYADCHLLLAGNECIRYGIGNQAEVVPFEQEADIG
                     FVTCDRTYDLDLVDDSFAYFFPNEPHQPNNFNQAGEAVTKCLIKVLMAD"
     gene            complement(36362..37192)
                     /locus_tag="VGY20_000356"
     CDS             complement(36362..37192)
                     /locus_tag="VGY20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ4273244.1"
                     /translation="MKKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(37205..38092)
                     /locus_tag="VGY20_000357"
     CDS             complement(37205..38092)
                     /locus_tag="VGY20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4273245.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(38197..39516)
                     /locus_tag="VGY20_000358"
     CDS             complement(38197..39516)
                     /locus_tag="VGY20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4273246.1"
                     /translation="MNMKKFASLAMLGASVLGLAACGGKSQKEASESKSDAAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKKFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMKKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39565..40269)
                     /locus_tag="VGY20_000359"
     CDS             complement(39565..40269)
                     /locus_tag="VGY20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ4273247.1"
                     /translation="MPDKPTKEKLMEQLKGEIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMTEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVIGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40543..40677)
                     /gene="rpmH"
                     /locus_tag="VGY20_000360"
     CDS             complement(40543..40677)
                     /gene="rpmH"
                     /locus_tag="VGY20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ4273248.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40992..41906)
                     /locus_tag="VGY20_000361"
     CDS             complement(40992..41906)
                     /locus_tag="VGY20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ4273249.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPVVNSADVEPEEIKATQRLEAED
                     TKVVPLMSEDSPAQTPSNLAETVTERKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41918..42745)
                     /locus_tag="VGY20_000362"
     CDS             complement(41918..42745)
                     /locus_tag="VGY20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ4273250.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIIFMGFNL
                     ASGVVLYWTVSNAFQVLQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42711..43070)
                     /gene="rnpA"
                     /locus_tag="VGY20_000363"
     CDS             complement(42711..43070)
                     /gene="rnpA"
                     /locus_tag="VGY20_000363"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ4273251.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43610..44350)
                     /locus_tag="VGY20_000364"
     CDS             complement(43610..44350)
                     /locus_tag="VGY20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4273252.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(44354..45637)
                     /locus_tag="VGY20_000365"
     CDS             complement(44354..45637)
                     /locus_tag="VGY20_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4273253.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKETVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            complement(45634..>46836)
                     /locus_tag="VGY20_000366"
     CDS             complement(45634..>46836)
                     /locus_tag="VGY20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4273254.1"
                     /translation="QFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDL
                     LSRFMGTIVIPYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKVICQSGYS
                     KRSHNFYQTLLIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYL
                     LSYFNHSSKEYLKNELRREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQA
                     IKSESITQIQDIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGI
                     DLNVEVPDNIKETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVV
                     TNTTKKKVDINKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLT
                     MPKEEVDR"
BASE COUNT        14146 a         9944 c         8502 g        14244 t
ORIGIN      
        1 tacactcctt ttttaatgag gtttcaatta gtttaataaa gattgtcatt ctctcatggt
       61 agcgttttat tacatcacta ctcttgtgag gtaagtcatt accttagaca aaaatatggt
      121 ataattaaaa aaataatact tgagtaagga taatctataa atgatcaaac gattaatttc
      181 cctagtggtc atcgccttat tttttgcagc aagcactgtt agcggtgaag agtattcggt
      241 aactgctaag catgcgattg ccgttgacct tgaaagtggc aaagttttat acgaaaaaga
      301 tgctaaagaa gttgtccctg tcgcctcagt cagtaagctc ttgacaacct atctggttta
      361 caaagaagtt tctaagggca agctaaattg ggatagtcct gtaactattt ctaactaccc
      421 ttatgaactc actacaaact atactattag taacgttcct cttgataaga gaaaatatac
      481 cgttaaagaa cttttaagtg cgttagttgt taataacgcc aatagccccg ctattgcttt
      541 agctgaaaaa ataggcggaa ccgaacccaa atttgttgac aaaatgaaaa aacaattaag
      601 acaatggggc atttccgatg caaaggtcgt caattcaact ggcttaacta accatttttt
      661 aggagctaat acttatccta atacagaacc agatgatgaa aattgttttt gcgccactga
      721 tttagctatt attgccaggc atctcttatt agaatttcca gaagtactga aattatctag
      781 caaatcctcc actatttttg atggacaaac catttacagt tataattaca tgcttaaagg
      841 catgccttgt tatcgagaag gcgtggatgg tctttttgtt ggttattcta aaaaagccgg
      901 tgcttctttt gtagctacta gtgtcgaaaa tcaaatgagg gttattacag tagttttaaa
      961 tgctgatcaa agccacgagg atgatttagc tatatttaaa acaaccaatc aattgttgca
     1021 gtacctttta attaattttc aaaaagtcca gttaattgaa aataataaac cagtaaaaac
     1081 gttatctgtc ttagacagtc ctgaaaaaac tgtcaaactt gtagcccaaa atagtttatt
     1141 ttttatcaaa ccaatacata caaagaccaa aaataccgtc catattacta agaaatcatc
     1201 cacaatgatc gcacctctat caaagggaca agtcttaggt agagcaaccc ttcaagataa
     1261 acatcttatt ggacaaggtt atctggatac tcctccttct atcaatctta tccttcaaaa
     1321 aaacatttct aaaagtttct ttttaaaggt ctggtggaac cgttttgtga ggtatgtcaa
     1381 tacctcttta tagaataaca caatgctttt tgtatgacat tgactgacag agtacttctg
     1441 cagtcttttt catttttatt taagcaaact aggccaatcc taacaatttt tcaaaaaaac
     1501 ttcacattgt attagcaaag tgatatactg aaaatgcatt acatttaaag gagtcggaaa
     1561 ttgaccaaaa gaattatact aactattttt acctttattt gcttttcgat gatgcctctt
     1621 gtccacgccg aagatgtcat ggatattaca aggcaagcag gctacacagt tagtgaggtt
     1681 aatcgaccaa aatcttctat tgtcgttgat gcaaatagtt cagatattct ttggcaagat
     1741 aatattgata ttcctaggga tcctgctagt atgtctaaga tgtttacgct ttacatttta
     1801 tttgaagagc tcgctaaggg aaaaattacg atggacacta ctattacggc aactccaacc
     1861 gatcaagcta tcgctaacat ttatgagatt agcaataata atatcgtcgc tggagtagct
     1921 tatcctattc gtgatcttat tactatgaca gctgtgcctt catcaaatgc agcaactgtt
     1981 atgattgcca attatttatc aaacaatgat gcttctgcgt ttatcgatcg tattaatgcc
     2041 acagccaaac aattaggcat gactaacact catttttcaa acgctagtgg tgcagcagca
     2101 caagcttttc aaggttatta taatcctact aaatatgatt tatctgcttc aaatattacg
     2161 accgctcgag atttgtccaa gttactctat gccttcttaa aaaaataccc tgaaattatc
     2221 tcctttacaa ataaatctgt tgtgcacact atggtaggaa cgccatatga agaagaattt
     2281 cacacctata accactctct cccagataat caatttggta taaaaggagt tgatggatta
     2341 aaaacaggtt ctagtcccag cgctgctttt aacgccatga taactgccaa aagaggcaaa
     2401 actcgactca ttactattgt catgggggtc ggagattggt cagatcaaaa cggagagttt
     2461 tatagacatc ctttcgttaa tgccttgact gagaaagggt ttaaagatag taagacctta
     2521 tccaaaaaag cacgccaaaa actcgaaaaa ttagtgccac aaaccaaaaa agaaacatca
     2581 cctaaacagc agcacttcaa agccactaaa aagcaatcgt acttggaaag agtcgaagat
     2641 tttatgaatc acaaccatac tttcttgctt atttgcttag ctatctttat aattactatc
     2701 cttttactaa gcctcgttgt ctttgcaatg ggacgccaat aatgaattat caaaaacctc
     2761 tgtcaaagca cagaggtttt tgatatgtta tcttacgtgt ttaaggcaac taaaaactaa
     2821 ccacttgggg ttagtactac aactcataaa atcctatctc aaagatcttt cagatggata
     2881 agagaaaatg caattaaccg acagaacctt ccatctcata ggaaatcaat cgattgagtt
     2941 cgaccgcgta ttccattggc aattctttgg taaaaggctc cacaaatccc atgacaatca
     3001 tctctgtggc ttcgctttct gataagccac ggctcatgag atagtagagt tgttcttcag
     3061 aaatcttaga cactttggct tcatgttcta acgccacctg tgaattatga atttcgttaa
     3121 atgggatcgt atctgattta gaaatatcgt ccatcaaaat agtatcacat tcaatatggg
     3181 aaacggattt tttagattgc ttgttaaagg tcacttggcc acgataatct actttaccac
     3241 cagacttggc aattgatttt gacacaattg atgatgatgt atgtggagca ttgtgaatca
     3301 ttttagcccc tgtatcttgg tgttggcctg cgttagcaaa ggcaatagac agcatggtgc
     3361 cacgcgcacc tggcccatca agataaacag aagggtactt catggtggtt ttagctccta
     3421 gattgccatc aatccattcc actgttgcat ccgtaagggc gcgtgcacgt tttgttacta
     3481 gattatacac attgtctgac cagttttgaa tggtggtata acgcatgtat gcaccgtcaa
     3541 gcgcaaagat ttcaacgata gcagcatgta agctgttact tgaataagtg ggagctgtac
     3601 atccttcaac ataatgaaca cttgctcctt catcaacaat aatcaatgtc cgctcaaatt
     3661 gaccagtatt ttcattgtta atgcggaaat aagtttgcaa aggaatatct accttgaccc
     3721 ctttaggaac ataaataaag gtaccaccag accaaactgc tgaattgagg gctgctaatt
     3781 tgttgtctgt tggcggaacc agtttggcaa agtattgttt gaacaaatca ggatattctt
     3841 tgagggcgga atcggtatct gtaaagataa tccctagctt ttcaaattca cccttcatgt
     3901 tatggtaaac cacttctgac tcatactgag ctgatgctcc agcaagataa gcacgttctg
     3961 cttctggaat tccaatacga tcaaaggttt cttttatttt ttctggaaca tcatcccaag
     4021 aacgagctgg cttatcagat gctttttgat agtaaatgat atcatcaaag ttaatatctg
     4081 ataagtctgc tccccaggtt tgcatcggca ttttattaaa ggtttccaag gattttaaac
     4141 gaaactccaa catccactca ggttcatttt tggcagctga tagttcgcga actactgcct
     4201 cactcaatcc ttttcctgta gaataaatgg gctctacatc atcgtgaaat ccaaattggt
     4261 agtcccctaa gtcaattggc tttggctcta ctttctcatt tatatcagac atttttccct
     4321 tcctttacat tttggtccgt gaggtgctga gcattggcac ttcgtttaat ggcttccttg
     4381 agagcattcc aagctagggt agagcatttg atacgctgtg gaaattttgc aactcctgcc
     4441 aacaattctg cttcacctag ctctttttga gcaggatttt cctgtccttg taccatctct
     4501 gaaaaaatat cagctaacgc gagtgcttct tctttacttt taccaataac agcatctgtc
     4561 atcatgcttg atgaagctgt ggaaatggta cagccgttgc ctgcaaaagc aatatcttca
     4621 attttatctt cgtcaaattt aacggtcaaa gaaatcacat caccacaagt cggattattc
     4681 agttgaacag cctctacgcc atctagttgc ccatgatgat gtggacgttt cgaatggtcc
     4741 gctaccacag ccatgtatag atggttcagt ttagagagtg ccattgaaaa actcctttgc
     4801 ttttagaatt gcttcgacta aacggtcaca atctgccttg gtgttataga tataaaaact
     4861 tgctctaaca gttgctggta cacctaaata actaagaaga ggttgggcgc agtggtgtcc
     4921 tgctcttact gcaacacctt catagtccaa ggctgttgcc aagtcatgag gatgcaaatc
     4981 atccagatta aaagaaatca gaccagatct tgcactaggc tggcttggtc catatatggt
     5041 aagcccctca atagcttcta atttcggcaa aacataggct attagttctg cttcatgcgc
     5101 atgtatatca gccatgccta gtctctgaag gtaagaaatg gctgcgctta gcccaatagc
     5161 accagctatg tgaggtgttc ctgcttcaaa cttccagggc aattctttcc aagtggcctc
     5221 ttgttcgtaa acaaaatcaa tcatttctcc gccaaattca agaggctcca cttgattcaa
     5281 aagctcttct ttgccgtaaa gaacacccaa acctgttggc cccaacatct tatgagctga
     5341 aaaagcaaag aaatcacaat ccaagtcttg aacatcaata gccaaatgtg gaaccgactg
     5401 ggcaccgtca acaacaaggt aggctccttt agcatgtgcc agcttggcaa tttctttaat
     5461 gggattgatg caaccaagaa cattggagac atgtactagg ctaacaaaac gtgtttttgt
     5521 cgtcaatttg tttgccaaat cgtccatgtc aagttggcca tcttttaaat aaacatagac
     5581 taaccttgct cctgtttttt gacaggcttg ttgccaagga atgatattgg catgatgctc
     5641 cataatcgaa atcaacacct catcttctgg cgtcaagacc tgctctgcaa accgagcaac
     5701 ccaattcaaa ctggttgttg ttcctctggt aaagagcact tccttgcttg atttagcatg
     5761 aataaagtca gcaacctgct ggcgactggc ctcatatttg tgcgttgcac gttcagccaa
     5821 ggtatgaact cctcggtgga cattagcatt atcttcttga taataggatt gcaaagcctc
     5881 aagaacaagc gccggttttt gtgtggtggc ggcattatcc aaataaacaa ggggttcatc
     5941 attgacttgt tgatttaaga tttgaaagtc ttgtttgatg tcttttgcgt ctagcaatac
     6001 taacctcttt ctttttggca gtaggtctta acgattaagc aatttctcat ctaaaacctt
     6061 aataatctct tggcggactg atggaatagg aatttcagcg ataaccgctc ctaggaatcc
     6121 tctaataacc aatcgttctg ctgtttcttg atttagtcct cgactcatca agtaatacat
     6181 atcttcaggg tcaacctgac cgatagaagc tgcatgacct gctgttactt cattttcatc
     6241 aattaagagg attggattgg catcggctct tgcttggtca gaaagcatca aaacacggct
     6301 ttcttgttga gcatcagctc ccttagcgcc ttttagaata tgaccaatcc cgttaaacgt
     6361 taaggtgcca cgttccaaaa tcacaccatg ctgtaaaata tgaccaaccg tacgttgacc
     6421 atagttggtc acgcgcgtgt caataccttg tacctgacga ccacttgagg ctgcaacaac
     6481 tttcaaatca gcttgtgagc cctgaccaat caaatcactg tcaaaatcag caatgacatt
     6541 gccttcattc atcacagcta aggcccaatc aatgttggca tccttctcta aacgtcctcg
     6601 acggctaata taggttgtca ctgaaggacc taagcggtcg atagccgaga atttaatctg
     6661 gctgcctgct tgagcaatca cttctacact gatattagcg ctggtctttt gagtggcatt
     6721 gccaatagat tcaaaacgct ctaaataggt gaacttactt tcttttcctg caatcactag
     6781 aacatgcttg ttaaaaggaa cgtcactgtc actatcttgt aagaaaatag cttcaatagg
     6841 agttgtgatt tccaagtgat caggaacgta gagcacggct gcgctattaa aataagcagt
     6901 gtggtaggca gctagtttgt cttcatcaaa agctaacgcc tgaccaaaat gagcttcaat
     6961 tacttctggg atttcctcaa gcgccgtata aaaatcactg aaaacaattc ccttgtcaat
     7021 taacgccatt ggtaactgtt ctaagactgt ttgcgtgcct acctgaacaa gctttgggtt
     7081 atctccaata gctataaaat ctggaacact agctagactt tcattttctg ttaaagtacc
     7141 atctcctaga ttccaacggt gaaatttaac cctttcgatg gttggtaact ctaattctgg
     7201 tatggcttct aatgccgcta aacgccgttc ttgcagccaa gcaggctcag cgtggacttg
     7261 cgaaaaagcc actagttttt cttttgtcat ctgtcaatcc tttgtctctg gtttttaaat
     7321 tggctgggta tatagaacat cacctagctt tttgtagaaa gctaaagtaa ttgtctccaa
     7381 aaacttaact ttcttcctta tactcgattc ccagatcctg tgcaatacct gcgtagcctt
     7441 ctttttcaag acgagtagcc aaggcagcat caccagataa gacaatacga ccatccatca
     7501 taacatgaac taggtctggg gtaatgtaat tcaacaaacg ttgatagtgg gtgataatca
     7561 tagctccaaa gtctttccca cgcatctcat taaccccttt agagactact tttagggcat
     7621 caatatcaag accagaatca atttcatcta gcaaagcaaa tttaggctct aacatgagca
     7681 attgaagaat ttcattgcgt tttttctccc caccagaaaa accttcattg aggtaacgct
     7741 ctgccatctc ttctttcata cctaacaaag ccattttctc atcaagtttg gtgataaaat
     7801 ctcgcacgga tatcttatcc tcatctgcct taccggcatt cattgctgca cgcataaatt
     7861 cagcgttggt aatccctgga atttctgaag ggtattgcat ggcgagaaaa agtcccagtc
     7921 tcgcacgctc atcaacttct agatcgagaa tattgacccc gtcaagcaaa atctgccctt
     7981 gggtcacttc gtagttagga tttcccataa tagctgctga aagggtagac tttcctgtcc
     8041 catttggccc cataatggca gctacttccc cagttttaag agttaggtta acccctttca
     8101 aaatctcttt accttctatt gaaacatgta aattattaat ctcaagaatt gacatgatac
     8161 accccttatt ttctctgtca tctataccat ctcaagaaaa gacagtgtca tttgcactgc
     8221 tttttttata atggtcactg catctattat agcaaaaaaa gccttactcg gcttttttag
     8281 tgtttcaaat ccttcttttc tttccatttc agaagcattg cttgacgata atcactatta
     8341 ccaataaact ttaacaagtt aaacaaaggc gttcgctttt caccccagat ctccaatcct
     8401 tcaataaaaa cttctaaacc aaataaaagt ccaagcatca agaggacgcc accaattcga
     8461 ctagaaacat ttaaaagtaa agaaataagg gaaaaaagca tggtaatacc ataaactact
     8521 aagacagctc ctcgatgagt aaagcccatt gataagagtc gatgatgcag gtgcatctta
     8581 tccggctcgt agaatttttg acctgataaa ctacgtcgaa taatagccac gatggtatcc
     8641 ataatgggta caccaagaat aataacaggt gtcaccacag ccacagccgt tgaatttttc
     8701 aatccttgaa gagacaaaac ccctatcata aagccaataa agagggctcc tgtgtcaccc
     8761 aaataaatca tagctggatg gtaattataa ggaaaaaatc ctgcaatagc agcaatcaag
     8821 accaaaatgg ttaacgtcaa aaagaaatcc ttttgaggta aaaagaaata agatacaata
     8881 gccatggtca ctaaactaat aatagagacc ccactaacca aaccatccaa accgtcaatc
     8941 aagttaatag cattagtgat ggaaacaatc cacaggactg ttaagaataa ggttaagaaa
     9001 ggaccaaaaa ctaacaacgg ccctccaaaa ggaattttga agctatcaaa tttaaagtcg
     9061 gtaaaagccc agacaatcac tgcaccaatt agaataccaa acatctttaa tttaggactt
     9121 aactcataca aatcatctaa aaaaccagta agggtaatta ctgtagcacc aatcacgaca
     9181 ggtaaaatat aactgaagta ggtctgacca aaaaacatgg cgcctttaga agcaatcgga
     9241 ataagtccca aacttgctac taaaaaagac ataaaaatag ctaatccacc actggttggc
     9301 atcggaacct tattgacccg tctggcattg ggattgtcta ctgcacccac acgaaaggct
     9361 aaaaagcgaa caagaggggt taagaataag gacattaaca aagcccctat cagaacaagg
     9421 acataatcta ttgtgaatga aaacatacta ttttatccca actcaatctt ttgtaattgc
     9481 tcaacagcac catcaagcat caattgtaag ccatgctctt gtaaataagc tcttgttttt
     9541 gatccagggt ttgcatgctc aatcaatctc gcatacatga cattcgcaaa ataagatggt
     9601 tgttgctgaa catctaataa aatagtcata tggtagcagt tgctcccctt atataactct
     9661 gaagcttcta tcgaaaaatc aatcgtttta gcaaaagcca ctgcctctgt aatcgttgaa
     9721 aaatccaata cataatggac atagtctaaa ggttctgact catgattagt tgatggatca
     9781 accgcttcag actgattagc tagagtggct tcaacaacat cttccattat ctcttcaatt
     9841 ttttccaatt tttcatgagc tttgacatct cccttttcac gcatggattg ctccaagctc
     9901 ttgaaaaaat cttccggagt catttgggac atatcaccaa actctgctaa atcttcaaga
     9961 ttaatatctt tatttatctc agatttggta acaaaaacat ctagacggtc cttgcgtgga
    10021 gtcactcgaa aactaagcat accactatct ttaaaattat ctggaagatc gagttcgtcc
    10081 atcacagaat agaaaaactc ttctgttttt tcttgaggaa tcaagaaatc tttcaactcc
    10141 attcctcttt cttctaaatc gtccatacta attgttattt ttagcgttgt ctcgctaatt
    10201 tgtttcattt ccattctcaa tacctcgcac gcgtagtcat tttattatac tagaaatgct
    10261 gtaatttcgc aatttatgac taactcctcg tctacaaaag gaaatttaaa aagcgacagt
    10321 ccccaaggtc tgtcgcaaca tcctaaacga tatgtaagtt taaaaaacaa atttaaagaa
    10381 gctataaata agtaaaaggc ttcctaacac aatacggtat tttccaaaaa tggtgaaatc
    10441 gtgtttctta acataatgtg tcaagaacct aatcgccaat aaactaacca caaaagcggt
    10501 caagctagcc actaaaagaa taaggacttg tgcaaaatca aggtgatggc catcaagaaa
    10561 aaatttcaca gcctttaatc cactatatcc aaacatggta ggaatggcca aaaagaaggt
    10621 gaaatcagcg gccactgttc gactagttcc taaaataata gctcctaaga tggtagcccc
    10681 tgatcgacta gtaccaggca caatacttaa gacttgaaaa caaccaatga agaaagctgt
    10741 tttgtaagac attctagcta agtcagtgac ggcaggttct tgttgagcat tttgtttttc
    10801 aatccagata aaggcaatcc cgtagacgat taaagcgatg gcaatcggaa ccataaaata
    10861 aaaatgtgct tcaaaccaat tatcaagagg aaccgctatt aaaatagaag gaatacacgc
    10921 aatcaccact tttagccata attgccaggt aagctgtact tctcttgctg ttttcccagg
    10981 ttggaaggga ttgagacgtt caaaataaat caacataaca gcgataatcg cccctagttg
    11041 aataacgata ttaaacattt ctataaaagc cttatcctga tttagtcgga taaattcctg
    11101 cactaaaatg aggtgaccag tgcttgaaac tggtaaccat tctgtgattc cctcaatgat
    11161 gccaaagaaa atggctttta gcaattcgat tattaacatg ttttctcctt attaaccact
    11221 cttattgtga ttttctaaac tgtgattaat gccaagcagc ttttttaaaa ggcaccgccg
    11281 ccgcctcctc cgccaccgcc agagaagcca ccaccaccac taatcccgcc tccggaagag
    11341 actgagaaat ttgaggaagt tgtagctgaa gacaagctag acacaaaggt tggtgtcgta
    11401 gcatacatta ccattgataa ttcacctggt cgaacagctt ggtaaacctc aggtaaggca
    11461 atacgatgga ctttcaaata gcgttccact ttcttagcgt aaccaaataa ggtggcataa
    11521 accaaaacac ggttccaaac aaccaaacct tcaatagcaa catcctcaaa tttgtcaatg
    11581 tctcgtatca tgtttttaaa gctctgccat tgatgtaacc gaacaccccc ctcaggtgtt
    11641 tcaataccca atttcttgta ttgatctaac ttcaggctaa tgtaaaagcc aaggcacatc
    11701 gttagcacca ttaaagcaag gtaaatgagg gccaacccac tttctttaat agccaagtaa
    11761 atcaataaac cactatttag aataagcaac aaacaaccta gtccctggac actattgctt
    11821 tttcgtttct ctgccggggt catttgacga taagtatctg gtaagtttaa ctttttgata
    11881 gtttctagga cattgtttgt aatagctgcc gacgcttttt tcatcgcctt aatcacctgc
    11941 tcagacgatt gacggacttc ttgctcgagc tttttacctt tgtacgtttt ctttaattgt
    12001 ttaatggtat cagcatcata atggtattga ctaaacaatt gatccaccgg caatgtcacc
    12061 ttgtttccaa aagccatatc taagaaggca gcttcctcat ctgtcacacg atctaactga
    12121 ctgatttcaa gataagcttt cccttcttct ttcgttaata aaagcacctt ttggtcgatc
    12181 agatccaaca aggtagcttg aacaagggcc tcaaaggtca ctcctttagg gatcaataat
    12241 ttctggcttt ctgatgctgt aggggataaa taggcaaaac tctgtccata aatcgcttgt
    12301 gccaacacaa gaggcgaaag gtcttcaggg acttcgtaac tgtgatctgt atggttggcc
    12361 aaatgataac gattgaattg tttctttaat cttgtaaact gtattaactg ccaaagaaga
    12421 aaacctacct ctaccagagg aatgactttg ccaaatagca gctgtaacaa ggcccttcgt
    12481 ctggaaatct tagtttctaa cgctacaatt ttattctttt tactcgttga aacatcaact
    12541 ggttcttctc ccaataatgc ttttttatcc caataagcat gcaattccaa ttgccctgag
    12601 acatttttgg cattaatctg atagcgactg ttaccttctt ttaacacttg aggtttcttt
    12661 tggtaatatc ctctgtgagc ccatagatta gaatcctgaa tatcagttgg ggtagtgacg
    12721 gtcaaactca ccttttctaa tattttgtcc caatcactaa tcggtgtcca attcagctct
    12781 gccacgtctt gataagctgt taaaaggtgg tgcagttgcc agataacctt gacgtcaacc
    12841 ttatcaccag cttggccagc attataaagt tttaaacgat agccatctcc aaggtcacta
    12901 aactcttgac taacaggtac ctgttgacca ttctgatata cctcaacttt aggcttttgg
    12961 tcaatagcaa agcccgctgg caaatgacct gtccggccca gcgaaatata ctggccgtta
    13021 taagaagtgt caaattgata ggttactttt tgctcaaacc gagcagtatt ttcttttgat
    13081 aacagtaact gaccttcata gttggtgatg ctatagtcta catcagctgc ttttattcgt
    13141 atccctaaaa gactgaagca gagcacaagc gtcatcaaaa tttttttcat ggcaacatcc
    13201 tctttctgcc ctcattttac catattttaa ggagatgaca aaacctaaac caagtccttc
    13261 atcttatttt taatcattat tttgaatatt tttccatatt tcaacctatt cactgataat
    13321 atattgaaat aggatgcaaa ttgctgtaaa ataggtctga atactatttt tgaaacagga
    13381 gacctatatg aaaaagctaa tccttagctg tttggtcgcc ttggcccttc tgtttggagg
    13441 aatgagccgg gctcaagcaa accaatattt aagagtcgga atggaagcag cctatgctcc
    13501 ttttaactgg actcaagatg acgcttcaaa cggggctgtc cctattgagg gaactagcca
    13561 atacgccaac ggttacgatg tccaagtcgc taaaaaagtc gctaaagcta tgaacaaaga
    13621 actgttagtc gttaagacct cttggaccgg tttaattcca gcattaactt ctggaaaaat
    13681 cgatatgatc gctgctggta tgagtcctac caaagagcgt agaaacgaaa ttagcttctc
    13741 aaacagctac tacactagcc aacctgttct agtcgtaact gctaatggca aatatgctga
    13801 ggcaaaaagc ctcaaggatt tttctggagc taaagtaact gcccagcaag gcgtttggca
    13861 tgtcaatctc ttaactcaac taaaaggtgc taagttacaa acaccaatgg gggatttctc
    13921 tcaaatgcga caagccctta cttcgggtgt tatcgatgcc tatatttctg aacggcctga
    13981 agccatgact gctgaagctg ctgatagccg tttgaaaatg atcactctta aaaaagggtt
    14041 tgctgttgct gaatcagatg ctgctatcgc tgtcggaatg aaaaaaaatg acgatcgtat
    14101 ggcaactgtc aaccaagtgc ttgaaggatt ttctcaaacg gatcgtatgg ccctgatgga
    14161 tgatatggtt accaaacaac ccgtggaaaa gaaagccgaa gatgctaaag catcatttct
    14221 aggccaaatg tgggctattt ttaaaggtaa ctggaagcaa ttcttacgtg gaactggaat
    14281 gacccttctg atttccatgg tcggaaccat tacaggtctc tttattggat tattaatcgg
    14341 tattttccgt acagctccta aagctaagca taaagtagcc gccttgggac aaaaactctt
    14401 tggttggtta ctcactattt atatcgaaat cttccgtggg acacctatga ttgttcaatc
    14461 tatggttatc tactacggaa cagcgcaagc ctttggtatt tcgatcgacc gtaccctagc
    14521 ggctattttt atcgtatcta tcaatacggg tgcctatatg agtgaaattg ttcgcggtgg
    14581 tattttcgct gtcgacaaag gtcaattcga agcagcaact gctcttggct ttactcacgg
    14641 acaaaccatg cgtaaaatcg tgctaccaca agttgttcgc aacattttac cagcaacagg
    14701 taatgagttt gtcatcaata tcaaagatac ttctgttttg aatgttatct ctgttgtgga
    14761 actttacttc tcaggtaata ccgtagccac acaaacctac caatatttcc aaacctttac
    14821 gattatcgcc attatctact ttgttcttac cttcacggtg acacgtattc ttcgtcatat
    14881 tgaacgccgt ttcgatgccg atacttacac cactggagca aaccaaatgc agattgcgga
    14941 ggtctcaaat gtctaactca attattgaaa tcaaaaacct caaaaaatct tacggcagca
    15001 atgaagtgct caaagatatc tccctttctg ttaacaaggg agaagtgatt tccattatcg
    15061 gttcttctgg ttctggaaaa tcaaccctcc tgcgctcgat caatcttttg gaagagccaa
    15121 gtgccggtca aatcctcttt catggtgaag atgtccttgc tgaacactac aacttgactc
    15181 actaccgtga aaaactaggc atggttttcc aatcctttaa cttgtttgaa aacctaaatg
    15241 tattggaaaa tgctatcgtc gctcaaacca ccgtcctcaa aagagaccgt gcacaagccg
    15301 aacaaatcgc taaagaaaac ctcaatgcgg ttggtatgac agaacaatac tggcaagcca
    15361 aaccaaaaca attatcaggg gggcaaaaac aacgggttgc cattgccaga gccttatctg
    15421 tcaatccaga agccatgctc tttgatgaac caacatcagc tcttgatcct gaaatggttg
    15481 gtgaggtcct taaaaccatg caagacttgg caaaatcagg gttgaccatg attattgtta
    15541 cccatgaaat ggaatttgct cgtgatgttt ctgaccgtat catcttcatg gataagggat
    15601 tgattgctga agaaggaagt cctcaacaaa ttttcgagaa tcctacacaa gatcgtacca
    15661 aagaattttt acaacgcttc ttaaaataag ataaaggatc caactatttc tatgccagtt
    15721 gggtcttttt tcaattcaaa aagaatttgt cataaaatac tttaccagtt aaatatttta
    15781 tgctatactt atttttgtta acgttaataa tttatattta ggaggataat atggcagaaa
    15841 aaactgactt atcgtcagct tacagaagac tgaaaagtcc caatattaaa accagaaaac
    15901 gtgctctgaa gattattcac gagtacaaac gttacggtaa aaaataagat tttacttttt
    15961 atctctccaa atttaagtca gacaaaaaaa ggctaagaca ccccttccat cttagccttt
    16021 ttgtatagag tttattaagg gaactatttt aaaatatctt ttatgctagg atctagaata
    16081 gttcaaaacg ttaaaatcat acatttaagg tagtgtttaa agtatacgaa aaagaactca
    16141 gcataaacca agctctcttt cgttataact aattatttag caatttttgc gaagtactca
    16201 agagtacgta caagttgagc agtgtaagac atttcgttgt cataccatga tacaacttta
    16261 accaattgtg atccgtcaac ttccattact ttagtttgag ttgcgtcaaa caatgaaccg
    16321 tatgatacgc ctacgatatc tgaagaaacg attggatctt cagtgtaacc gaagctatcg
    16381 tttgaagcag ctttcatagc agcgttgatt tcgtcaacag aaacgttttt gtcaagagtt
    16441 acaaccaact cagttactga tccagttgga acaggaacac gttgtgcagc accgtcaagt
    16501 ttaccgttaa gttctgggat aacaagaccg atagctttag cagcaccagt tgagttagga
    16561 acgatatttg cagcaccagc gcgtgcacga cgaaggtcac caccacggtg tggtccgtca
    16621 aggatcattt ggtcaccagt gtaagcgtgg attgtagtca taagaccttt ttgaataccg
    16681 aatgcatcgt gaagagcttt agccatagga gctaaacagt ttgtagtaca tgaagcacct
    16741 gagataactg tttcagtacc gtcaagaatg tcgtggttag tgttgaaaac aactgtttta
    16801 acatcgtttc caccaggagc tgtgataaca acttttttag caccgttagc atgtaagtgt
    16861 ttttcagctg cttctttttt agcaaagaaa ccagttgctt caagaacgat ttcaacccca
    16921 tcagttgccc agtcgatgtt ttctggatca cgttcagcag aaactttgat gaagtttccg
    16981 tttacttcaa atccaccttc tttaacttca actgttccat caaaacgacc ttgagttgta
    17041 tcgtatttca acaagtgtgc aagcatattt ggatctgtaa ggtcattgat acgagttact
    17101 tcaacacctt cgatgttttg aatacggcgg aatgcaagac gtccgatacg accgaaaccg
    17161 ttaataccaa ctttaactac cattagtgat ttcctcctta tgaaaatcaa aagaatatta
    17221 tttagaggga atgctcccta cctattgtga aaagattaac ttacagtcat ctgcaacaac
    17281 ctttcaacag aactattata taactatttc agaaaaaatg caaataaatt agccattttc
    17341 ataaagggca gcttctttct actagccttc aaaacccacc aaatatagga cagggctttc
    17401 ttttttctat gttctattct gtaatttatc tttttattgg gaaagatttc caaaacttca
    17461 aactgtacta taacgttctg ttacactaaa aaagaggtta ggatagttat cctcaacctc
    17521 ttagtggtgg ttattcacct ttgtttttct taatgatttc ttcttgaact gacttaggaa
    17581 catcttcata gtggtcaaat accatcatga atgtaccacg tccttgagta gcagaacgaa
    17641 gaactgttgc ataaccgaac atttcagcaa gtggaacata agcacgaacg atttggctgt
    17701 taccatgtgc ttccatacca tcaacgcgtc cacgacgagc tgtcacgtgt cccataacgt
    17761 ctccaagatt gtcttctgga gctgtaatcg ttacaagcat catcggctca aggatagctg
    17821 gttgtgctga tttagctgct tctttaagag caagagatgc tgcgatcttg aaggcagttt
    17881 cagatgagtc gacatcgtgg tatgaaccat cgtaaagttt agctttaacg tcaaccattg
    17941 ggtaaccagc aagaacaccg tttgccatag attcgatcaa ccctttttca acagctggga
    18001 taaattcacg aggaaccaca ccaccaacga tagcattttc gaattcgaaa cctttacctt
    18061 cttcatttgg tgtaaattca atccaaacat caccgaattg acctttacca ccagattggc
    18121 gtttgaagaa tccacgagct tgtgtagaag cacggaatgt ttcacggtaa gatacttgag
    18181 gagcaccaac gttagcttca actttgaact cacgtttcat acggtcaaca aggacatcaa
    18241 ggtgaagttc acccatacca gcgataactg tttcaccagt ttcaacgttt gtttcaacgc
    18301 ggaatgttgg atcttcttca gcaagttttt gaagggcaac acccatcttg tcttggtctg
    18361 ctttagattt tggctcaacc atcaactgga taactggttc tggaacttcg attgactcaa
    18421 gaataacttt tgctttttca tcagtcaatg agtcaccagt tgttgtgtct ttcaaaccaa
    18481 cagcggcagc gatgtcacca gcgtaaactg tttcaatttc ttgacggctg tttgcgtgca
    18541 tttgaaggat acgtccgata cgctcacgtt taccttttga tgtgttcata acgtaagaac
    18601 cgctattaag aacacctgag taaacacgga agaatgtcaa acgacctacg aatgggtcag
    18661 tcatgatctt gaaggcaaga gctgcaaatg gctcttcatc agatgctgga cgttcttctt
    18721 cagcatctgt atccgggtta acacctttga ttgcagggat gtcaagtggg cttggaaggt
    18781 atgcgataac cgcatcaagc attaattgaa cacctttgtt tttgaaggca gaaccacaaa
    18841 gtactgggaa gaattcaacg ttgattgttg ctttacggat accagcaatc aattcgtcgt
    18901 tagtgatttc ttcaccttca aggtatttca tcatcaagtc ttcatcagtt tcagcaactg
    18961 cttcgattaa tttttcacgg tattcttgag cttgttctaa gtattcttct ggaatatctt
    19021 cttcaaggat atctgtacca aggtcgttgg tatagatttc tgctttcatc ttgatcaagt
    19081 caatgatacc acggaaatca tcttctgcac cgattggcaa ttggattggg tgtgcatttg
    19141 cttgaagacg gtcatgaagg gtttgtactg agtaaaggaa gtcagcaccg attttatcca
    19201 ttttgttggc gaaaacgata cgtggaacac cgtattcagt tgcttgacgc caaactgttt
    19261 cagtttgagg ctcaacacct gattgtgagt caagaacggt tacagcacca tcaagaacac
    19321 ggagagaacg ttgtacttcg atggtgaagt ccacgtgtcc tggtgtgtcg atgatgttaa
    19381 cgcggtgacc atcccattga gctgttgtag cggcagaagt aatagtgata ccacgttctt
    19441 gctcttgctc catccagtcc atttgtgatg cgccttcgtg tgtttcaccg attttatgaa
    19501 ttttaccagt gtagtaaagg atacgctctg ttgtagttgt tttaccagca tcgacgtgag
    19561 ccatgatacc gatattacga gtttttgcaa gtgaaaattc gcgagccatg tgtttctcct
    19621 atttttttag tttactcttt cattatagca ctatttagca aaaacggata ggcaggacct
    19681 acccgttatc taaatatttg ttggtaaaga taatacctat tgcctttata aggcttggta
    19741 tcttattcat tgaactcagg ctaaacgctg tgtgaaaaag ataaactttc ctagagacaa
    19801 aggtttcttc atcaaatttc ctattttcac tttgcgttct atacgccttc gtatcttatt
    19861 gattgagttc gagctacaaa ccactgacaa aaagataata gttcctagaa actgaagttt
    19921 ctgcgtcact attcctattt tgtcttggac ttgattaaca ctctcacatc ttatattagt
    19981 tgaattttag ctacaaatgt ctgccaaaaa gataaaatta cctagaaact gaagtttctg
    20041 cgtcaatttc cctattttgg ctagcatttg tttgacgcta aaatatctta tattaccaac
    20101 ggaagtgagc aaaggcacgg ttagcttcag ccatcttgtg agtgtcttca cgtttcttaa
    20161 ctgatgcacc tgtgttgttt gccgcatcca tgatttcttt agcaagacga tctttcatag
    20221 tgtgttcacc acgagctcgt gatgcgttta ccaaccaacg aagtccaagt gttgtacgac
    20281 gttctggacg aacttcaact gggacttggt agttagaacc accgacacgg cgtgcgcgta
    20341 cttcaagtac aggcatgatg ttgtccatag ctgtttcaaa tacttcaaga gcgtcatttc
    20401 ctgtcgcttc tttgatagcg ctgaaagcat cgtaaacgat agtagcagct gtaccacgtt
    20461 taccgtcaag cataacacgg ttgataagac gtgttacgat ttttgagtta tataatggat
    20521 ctggtaatac ttcgcgttta ggcgcttgat ttttacgact cattttctct tatccccctt
    20581 ctattatcct tttggacgtt tcgcaccgta tttagaacgg ccttgtttac ggtcagcaac
    20641 acctgcagta tcaagtgcgc cacgaacgat atggtaacgt acccctggaa ggtcttttac
    20701 acgtccgcca cggataagaa caacgctgtg ctcttgcaag ttgtgtccga tacctgggat
    20761 gtaggcagtt acttcgataa ggttgctcaa acgtacacga gcgaatttac gaagggctga
    20821 gttaggtttt ttaggtgtca ttgttccaac acgagtagca acgccacgtt tttgtggggc
    20881 agccatttta gtttgaactt ttttgtgact gttgtaacca atgttcaaag ctggtgaatc
    20941 tgatttttca attttagatt tacgtggttt acgtaccaac tggttaattg taggcatcta
    21001 cattctcctg tgttttttta tttttggttg atatagcact tggtgacagc ccatatccgg
    21061 tgcacttttt gcaacaattg tcagcacgtc tctgtacact cttgagagac caaaagtaaa
    21121 aagtaccgtc taatataata acacagacgg tacttcatgt caaatagaat tttttctttg
    21181 atgcgattgc ttataacgaa tgcatcacct agattcctta cggaacctaa atcctgtgag
    21241 gccgacagct gccaacataa cgactcctgc tttaagagct ctttgagtac tttcatcaac
    21301 atcagaaatg agattgctcg ttgtagaaga tcctgagcca taagatgtct tagatgaggg
    21361 ctgagtaatc gaagatgtga ttttagacac aatggcatta cttgctacca tttcagaggt
    21421 ttggccaaca agcgcttctg ctacaagact tgtattttca gcagctaatc ttgctgaagc
    21481 ttctaatagt tgtttcgttt ctttaaccat ttcttgaaca agcggagtag tatctatctt
    21541 actatatgat agcggtttta cgcccgtaag aggtggagct acttgcaaat caggcacagt
    21601 agctatatct tcgtctaaat ggcgatattc cttttcgtta gctaaggttt taagcaaagt
    21661 caactggtgt tctgtggtag caatagtagc ttctagactg ctttgtttag cttgtaaggc
    21721 tgctaaagct tcttgtgcat ttaacaaaga tgaggtagtt ttagccaaat cttgcttagt
    21781 attatcaatg cgctcacgta tcacttgaag gcggttagga ttcaatttaa agtcccttag
    21841 atattgcaaa tgagcttttt tagccaccag tgctgtcact ctggctgcgg cttgctctgc
    21901 taaggcttct gtctggtgca gtgcggcttt caacgatgct agcttagcca atgattgatc
    21961 acgagtagct tcgagttgtg cttgttttgc tttagctgct agtaattctg ttctcaaaga
    22021 acctttagta tcatttaatt gtctcacttg gaggtttaag ctgtctgact gcttaagtgt
    22081 gcttgctaat ttaccttgaa gttgacttac tttagcttgg gctgccataa tatcagcttc
    22141 ttgatgaata gccgacaaac gattttctaa tgagcttact tttcctttga tcgctgcaat
    22201 agtatcagat acagtgccta ctctttgggc ataatctttt gtacttccaa cggcttttat
    22261 aggggtctta ttaaagcgtt gatggttagc aatgttagac tctggaaaca ttacaaagtg
    22321 ttcattcaaa gatcctacat tgctggttga aaatccaagg taaacaggcg cattagggtt
    22381 acgtttatct acacgtaaaa agttaatagc atggccgtat gtatttccgt gtaaatgatc
    22441 tgtaaagagc atatacttga tactgtcata aataccacgt ttaataccat tcacagtatg
    22501 cacatcgtta aaagcaccga tattctcgta catgttatca tcatttcgaa tgagccctga
    22561 cgctccggca gagtcttcaa taatagtttt atcatgaggc ccaacaccat aatgccctga
    22621 tacccctggc tgtccgtaga caaatgatgg tcttgtatta ccatgagttt tcttatagct
    22681 ggtactaagt aatcttgcaa attcttgtga tcctgctgtg acagtaactg gtggtagacc
    22741 taattgtctc cttacactat taatcatgtg agctgcaaac tgcgctagct cattttgcac
    22801 ttctggtgtc aaattatcgg gatcaacaaa gcgattacga tctgctggaa tatcttggta
    22861 ttgatttaat tgattacctg gactagcttt ggcaataatt tgatctgcat gctctttgta
    22921 ataattattg taactagctg atccaatata accactagct tctaattttt taagttcttc
    22981 aagaggatag ccttgcggtg ctttcatggt attattaccc acaatgctat cttgagtaga
    23041 cggagctgag gatttaagac gactaagttc tgcctctttt tctgctagag cagctttctg
    23101 agctgccaac tcttcagtca attgcttttt aactttagct ttttgatttt ctaagtcagc
    23161 tttagccttc tccaattctg agcttaatgc ttttgtatta tcattagccg tttgagctgc
    23221 tttagtgata gcatcaggat tgctaatcat agcattgagc ttagcaatat tttgttcaga
    23281 cgttttgact tgttccacta aatcttgagc tcgagtagtt tctgctgaaa tgctagcttt
    23341 ttgttctgac aatgcagtct cttttgaatg ttgatctgct tgagcattat gaagctctgt
    23401 ttcagtagct gttaactctc tttgatgttc ggctccttgg gctaatagcg tctcctcact
    23461 acttgcaaga gtattagtgt aaatttcttg tgcagaggtt aaagcttttt gttcattatc
    23521 ttgctgctct tttaagtggt tgatttcagc agtagttttt gtcagagcgg tagcaagctc
    23581 tgtcagtttt gttttttgtt gactgagagt tttttcaact gcttcaatag ttgcctttgc
    23641 ttcttgaatt gtttctggtt ttggtaaact atcgtcgtga gtattactcg ccttcgtttc
    23701 ttctgaggct ctatcatctg ctttgacttg atgagatacg cctacactgg cactcaataa
    23761 agcaattgtt gaggttaaag caattttctg cttaacttgg tttggcttcg tttgttctaa
    23821 gtccattctc tctccttttt ccatttatcg aaaatattac caacttctac aacaacattg
    23881 ttgtttttga catcaatctc tgaaactttc agcaatgatt tgaaggtcat ttgctcacgc
    23941 tctgattgag cgttttctgc aaacacagca acaccaacta aagtgctgtc aaattctgtc
    24001 aagagactga tcattcctgt aatcgtcccc ccgcccttca agaaatcatc tacaatcaat
    24061 acacgactat tgggtttcaa actacgcttt gacagaaaca tcttttcaat acgatcacta
    24121 gaagcactcg cataattgac agacactgtc gatccttctg ttatttttaa atcacgtcta
    24181 acaatgacaa aaggaacact taaaatatta gccactgcat ttgctaaagg aactcctttg
    24241 gttgcaactg tcatcacagc gtcaatcttt tctcctttaa aggcattggc aataatacga
    24301 ccaatatttt gtaaaatttt aggtgtactc agcaagtctg acaaatagat atagcctcca
    24361 ggtaagatgc gatcactctc tgataacctt tgacacaggt cttcaacgat ggtacgcgct
    24421 tctgtttctg aaatactggg ggtaaaaatt acgcctccac ttgctcctgt aagtgtatcg
    24481 atatcaccaa tgttagcttc ttcaaaagct ttcttgataa tagcgatatc ttctgagata
    24541 gaagattttg cggcctcata tttagttgca aaggtgttta aactagttaa tttatacgga
    24601 ttgttaatca aataatttga aatgacaacc atacgttcac ttcgtcttaa tttcatagtt
    24661 tccccatttt tatgtacttt ttgctattat accataaagt taataaaaaa cgaacattcg
    24721 attaggaaaa tattggtttt tttgttctta ttaatatcat gcaagaattc aatctaagat
    24781 aaactaatca acattcctac tgaaaggaca ttctacttaa taaccataac aagagccaaa
    24841 aacacttatg aaaccatcat aagtgttaag cacatcaaaa atcatcacaa aaacaaagga
    24901 actattactt gagaaacact gatcaatagt ttggcttgta aaaagaccga ttatccatgg
    24961 caaaaatccg attggtcatc tcgccctcgc taacacggct aagagctgtt gtcatcatca
    25021 tcatattagc atcaatattg tcaatcatat gaataatttc agcttccatg atgcgtggac
    25081 gtaccggact accgtattct aattgcccgt gatggcttaa aatcacgtgc ctaaggacaa
    25141 tgacttcttc tttggtatca tcgatttgta gttctgaaat tacttttgta atttcttcgt
    25201 taatgaggga aatatgccca atcaagttac cacgaacggt gtattctgta ttatctggtc
    25261 ctgttaattc tatgacttta gctaagtcat gtaacataat acctgcaaac agtagactct
    25321 tatttaagtc agggtagata tctccaatgc tatctgctag tctgaccatc gtagcagtat
    25381 ggtaggcgag gcctgactca aaagcatggt ggtttgtttt agctgcagga taagtataaa
    25441 attctttatc atacttacga taaagagccc ttacaattcg ttgccacgtt gcattttcaa
    25501 ttttaaagag catctgttct aaatagtcac gtacttcttc aacactgaca ggtgcctttt
    25561 ctttgaagtc ctttggatca ttgggctccc ctgggcgaac atttcgcaag gtgatttgat
    25621 taacctgagg ggtaccatta tagacctcgc gtcgcccttt cataaacacg actttaccag
    25681 cggtaaattc ttcaacgtta taaggttggg catcccacag atttcctgaa atctcgccag
    25741 tgtcatcctg aaaggtaagg ctaataaaat ctttaccagc ccttgtctta cgaacctctg
    25801 ccgacttaat gaggtagaac ccttcaaaaa gctggtcttt tttcatttga ttaattttca
    25861 ttattttcct cttctaataa gggcatgttc aagagagatt ttgttgcttg gtcttgataa
    25921 gtttcaacgg tattcaaggc tcgaacaatg gcatttgtcc ttgttgaaat gagctgatcc
    25981 agcgtattat tagctgtatt catttgcttt tgagccttgg caagcaggcc accaaattta
    26041 tcgaattcta acttgacatt gcccaaaatt ttactgatgt catcagcatt tttttggata
    26101 ttaagggtct tgaaaccaac agataaggaa ttgagcaaag cagacagggt cgaaggacct
    26161 gcaaccacaa tattttcttc ccgacgaagg ctatcaaaga aagccgcatt tctgaccact
    26221 tctgaataaa gaccttctgt tggtaagaac ataactccga aattggtcgt ctctgggggg
    26281 ttcaagtact ttttatgaat gtctttggca aagcgtttga tagctgccag aagtgctttt
    26341 cggctagcct cgatggccag tttatcacca acttcgtaag catcttctaa tcggtaataa
    26401 tcttcaagag ggaattttga gtcaatcggt aggtaaatat aaccgccttg accatttcct
    26461 gggagcttaa tcgcatattc tacgcgttca ctagaaccac taaccgttac aaattctctt
    26521 tcgtactggc ttgatgtcat gatatcctcg atgatttggc ctagttgaag ttcgcctaaa
    26581 atgcctcgtg ttttggtatt ggacaaaacc ttatttaaag tacccacatc ttgagccacg
    26641 ctacgcattt ctcccaagcc tttattgaca ctttctagtt gcttggatac agaatcgaaa
    26701 gaggcgtgca gacgattttt taaggtttct tctaattttt cttcaacgat ctggcgcatt
    26761 tcctccaaac gtttttcgtt agattcttgc atatttttga gcgattggtt gacctgctgg
    26821 ttaatttttt ctaagcgttt gtcagaccgg tccctactat cagacaaact acggtgcaag
    26881 acgtcacgaa tatctgttaa ttgttggtaa aggtctgctt gttgtcggtt catcagctgt
    26941 gttagctcta acaattgttg cttgttagct gtatccaact ggtaggtcat ttgatcagac
    27001 aaattatccg cgttgccttc tagggtttgg gcaagctgat gttgaaggcc attaactttg
    27061 aacaacagat aagcccctaa acctaagaga accaagacca aaaggaacaa gataaggtcc
    27121 atactaactc caatctttac tttgaataat aatcaagtag ccacttggca aactaactgt
    27181 aataggttga tggataaagg cattactgga atagattttc tttttgaaaa aattatcttg
    27241 cgtcaactca aatttagcac cagtaatggt caagtcagct tctccttctg ccataaatgc
    27301 cacataggtc atcccttctt cttgatggat aaggtgctga cctgccgggc ggtaggtaat
    27361 catattttgt tggtcacgca aggcgatttg tgccataaag ggagcaatac cagggtcgct
    27421 aggcaaaaaa atattggata agaggtggtc cattcgaccc ccaaaagccc caaaaatggt
    27481 cacttccgcc tcaggaaaac gagcaaagac ctctttaagg gctaattccg tatccgtatc
    27541 atttttttct ggattagcag tgatgagaag ctctgccttc tcttttatat ccgtaaatgc
    27601 tttttgggaa accgaatcaa aatcaccaac agccatattc aaaggaagcc cattttctaa
    27661 taaaaatagg ctccctctat caataccgac aaaataatca aaatcacggc tgatataaga
    27721 gagatctcct cccgcaaaaa gagcaacttt agacattcaa ggctgtcctt aaggtttgga
    27781 cttggctcac taggtcactg gccttgaaaa ggtaagaacc tgccacaaag acattggccc
    27841 ctgcttcata acaagcacga atggtcttat tgtctactcc gccatcaacc tcaatgtcaa
    27901 aggacaatcc tttttcatct cgccatttgg ctacggtagc cactttttct aaacattctg
    27961 ggataaaggc ctgaccccca aaacctgggt tgactgtcat aatgaggact tggtcaacta
    28021 agtctagcaa aggttctagg gctgttgcag gagttcctgg attaatcact acccctgctt
    28081 tcatgccagc tgccttgatt ttttgaaggg ctccatgaat atgacgagta ctttctgtgt
    28141 gaatggtcat gatatcagca ccagcttgag caaaagcctc aacatagcgt tcaggatcaa
    28201 ccaccattaa atgacaatca aaaaccaatt tactgtgctt tctcatactt gccacaacat
    28261 ctgcaccaaa actgatgtta ggaacaaatt gaccatccat aatgtcaatg tgaacatatt
    28321 cggcatctgt ttcttcaata cgagctagct cagaagcaaa attagcgtaa tcagctgcta
    28381 gaatagatgg ggcaattttt agagttgaca tggagtagac ctactttctt ttgataactt
    28441 ttttatatgt ttcacgacgg ttttcaattt cactgagaaa ttgcaggtaa tgctcataac
    28501 gaactggcca caactctccc atttcaagcg ctgccttgac agcacacttg ggttcgtggg
    28561 tatgggtaca ggatctaaac ttacactcat ggctcagccg gcgcagctct ggaaaagcct
    28621 cgttcaaatc ttcagcatta gcaatatcat agtctaatga tgagaaacct ggcgtatccg
    28681 cgatttttcc gccatgggta ttgtaaaaac tcacagctcg ggtggtgtga cgaccacgcc
    28741 ctagactatc tgagatttct cctgtttcaa gagccaattc gggggcgatt cggttgagaa
    28801 gggttgattt tccaacacct gtttgtccca taaagacggt aattttatca gctagcaaag
    28861 gcaacaattc ctcaagactg gtcacaaaat tgtaaccgat tgcctgatac tgtcgaccaa
    28921 tagccttaat ctcctcagga ctatctaata agtccatctt agagatataa accactggat
    28981 gaatggcttt gtgttcaagc aagatgagaa agcggtctag aagattgctg ttaaattctg
    29041 gttctttagc agacataata accactgctt ggtcaatatt gacgatagga ggtcttacca
    29101 aactgttttt acgaggatga atcgctaaaa tataaccttc agagttgtcc tctgctgaga
    29161 aatccacgat atctccaaca taaggtgtct caccgcgctt cctaaaatta ccacgcgctc
    29221 gcgtctgata tacctgtcct tctgactcca cgtaataaaa accagccaga gatttaatga
    29281 ttttaccttg caactgtgct cctttgccct actataataa ctctattata tcagaaaacc
    29341 gctcccaaat cagcatttga gtctaagttt cactcgcttt aaaaactgtc aagattctct
    29401 agaccactgg ctatcattta ggtcactgct ttttaaaaat agaatatttt atcaactaaa
    29461 aatcagatca ctgatgattt tttgaggtag gaaacgatgt gaattttaaa aagacctttg
    29521 ctatttacga aaaagagtct aaccgtaccg ctatagtcac gctcttgtta attgcccctt
    29581 tttttccggg gtattaaaaa aactagctct gcactgcgat gccttaacgg tcagcttcaa
    29641 taagaaaaat ccccctgaac tgaacatctc tgttttgtct acaagggaat tttttattgt
    29701 caccggggga aagcttttat aatccaactt cttttaaagc atcagctaat ttgccaaaat
    29761 cttgaatcga caaggcctca ccacgaatag aaggcttgat atctgctagt gccagacctt
    29821 tttcaagttt agccttgata tcttcagact tgccaaaatg gctagtcaag ttattccaca
    29881 gggtcttgcg acgatggaca aaactgagac gagacactct gaaaaagaaa tcttcgtcct
    29941 caacctcaat caaaggctgg tcgcgacgca ccattttaag gatagcacta tcgacatttg
    30001 gtgcaggtac aaagacggtg cgcggcacaa tgaaggcaac cttggcagtc atgtaatact
    30061 gcaccgcaat ggacaaggaa ccataagctt tagtgtttgg ctcagccgag atacgatctg
    30121 ccacttcacg ctgcatcatg accacaaact cttggaatgg aatcttgctc tcaatcaagt
    30181 gcatgagaat aggcgttgtg atgtagtaag gcaggttagc caccacctta atgggcaaat
    30241 caggattttt aaactgcttg atttgggtct gcaaatcagc ttttaaaata tcttgattga
    30301 ccacttgcac attatcaaaa tcacgcaagg tatctgccaa aatcggcacc aagcggtcat
    30361 caatctcaaa agccatcact tcagcagcat tttctgccaa aaattccgtc aaagccccaa
    30421 ttccaggacc aatctcgata acattgacat tctgatcaat ctcagccgtg tcaacgattt
    30481 tctgtaaaat attggtatcg gtcaaaaagt tctgaccaaa agatttctta aaggtaaatc
    30541 cgtgacgatc aaggacagtc ttggtcacgc tataatctgc aattctcata tattctcttc
    30601 ttttattctc ttctttttat gatcactttc tttttggcat ttgaaccact agcctaaact
    30661 tttttgccaa gacacgccat tcagttaatg gggctgttat attttattgt actctttact
    30721 cctcatatgc ctccatcact tcttccactt ctgctagggt aatgccaaat aattcgaggc
    30781 gcttgagcag ttgtttgcca ttggcatagc cgatgcggag cttttcgccc aagtattccc
    30841 tgcgtttacg gctatctgac gccattaaaa gccctagacg aatcaggtct gtttgacgaa
    30901 tgtcaaaata agactcatca tcatattgct gagtgacatg agcaagtgcc ttttggaggt
    30961 cctcaaaatt ggcatgttca acccctaagg aacggccctt actcttagaa cttggagtag
    31021 cctcatttcg gtttaaaaag gcgtgacgag ctgttggcac agctgccata atgagcttac
    31081 gaatgcgctc accattataa tctgggtctg tcaaaacaat gacaccacgc aagtcattta
    31141 ggcggttaat ccgttctaaa tcttcttcag tgatagcaga gcctctggtc tcataggtat
    31201 ccacctcgta aaaacggcgt aggttagccg tgtcgtcctt gccttctaca acaagaacct
    31261 cttgaatatt aattttttca gtcaagcttg aataccctct ttgcattagc agtcgttgct
    31321 tttgcaactt cttccacggt cattccacgc agctctgcga ttttatcaac cacatagcgg
    31381 gtataagctg tatgattttg tttgccacgt tttggaactg gtgtaagata aggggcatct
    31441 gtttccacca caatcttatc caaaggcaag tgctgcgctg cctcttgaat atcaagggcc
    31501 tttttgaaag tcaccacacc tgaaaaagaa atcatcatac ccagatccac aaaacgccga
    31561 gccatctcta atgaccctga gtaagaatgc atgatgcctc cacgagggcc aacaccagct
    31621 gctttgatga tctcataggt atcttccaag gcatcgcggg tgtgcaccac aaaaggcaaa
    31681 tcatggtctt ttgctaattg catttggcgc ttaaaaacct ctatttgcac ctctttgggg
    31741 tcctccatcc aataatagtc taagccaatt tcacctaagg caatgacttt aggatgtgat
    31801 aattgagaga cgatagactc ctcaactgct tcagaataag atcctgcttc tgtcgggtgc
    31861 catccaatag tagcatacac gttaggatac tggtctgcga gagcaagtgc tccactaata
    31921 gtggcctggt caaagccaac cacattatgg taggccacgc ccatttcttg agccaaagtc
    31981 aattcttcag tttcgtgtcc ttgaaattct gctacattca ggtgcgtatg ggtatcaaaa
    32041 atagttagtt tttctacttt atttgtcatg atgttattat aacataaaga tcttaaccac
    32101 atcagaaaac ttggtgagaa cttctgccgt ttaggttgat cgtaaaaccc tttgctagct
    32161 gacacttgtc tattttctac ttttttgaca aatgaccttt aatagtcggc tcctacataa
    32221 gccctcaaaa atactacaac ctactcaaat aaagtgggtt gaacaaatct gtatcacaaa
    32281 aagggtcgct accttctgtt agcgatctat gctatactag tagatagtta aactgtaagg
    32341 agtgtgcttt gctatgccta aaaaccaccg cctaattact aaaatcgaag catcattgga
    32401 acatatgact agtttagaaa aagggattgc tcattttttt attacaaccg atctgacacc
    32461 tcaagagttg accgcatccg agatagttaa gcgactacac atctctcaag ccgccttgac
    32521 tcgatttgca aaaaagtgtg gttttactgg ttatcgtgct tttgcctttg attaccttca
    32581 cagtcttcaa gaatcacaag agactttcca atctattcac ttagaattga caaagcgcgt
    32641 gctgatggac tacgatgctc ttattaataa aacctatgag ctcgttaatg aggaaaaatt
    32701 actgaactta gccaagctga tcgatgcttc tgagcgagtc tatttctttg gcaaaggatc
    32761 ttctggacta gtagcaagag aaatgaagct acgctttatg cgacttggtt taatctgtga
    32821 tgcttattca gacactgatg gttttacttg ggcaaatagc ctcgtcaatg ataattgcct
    32881 tgtttttggt ttttctttat ctggcaaaac aaactctgtc atcacagcac ttcatcaagc
    32941 tagtcagcga ggggccaaaa ctgtcctctt aacaacagat aatcaaaccg aatttgacga
    33001 ctccttagat attattcccg tatcatcaac ccaccaacta cattatggga atcgtgtgtc
    33061 accacaattt cctcttctta ttatgatgga cattatttat gcttacgttt tagcgattga
    33121 caaaccgcac aaagaaaaaa tcttcaaaaa cactattatt gacaaagaaa ataacgacct
    33181 ttagactaca atagcaaaaa gggcgttgtt ttctttttaa agggtcgtca ttcacctccc
    33241 ccagtatact taaacaagaa ctcttcacgt catatagact cacagccact aagattagct
    33301 actcgctaaa ttattttcga ttttctcagg agttataaat gaacaggatc gctcattctt
    33361 ttgtttaaaa tggtagtaag ctcctaatat accagcatta tttccatgac tagcaaactt
    33421 cagctgagtt ttcttggcaa gactagaaac caagtagcta tcaagagctg tctttaattt
    33481 atcagctaaa taatcttttt gcgccatgat accgccacca agtaccacca catttgggtt
    33541 aacaacgtag cagatattag caataccttg tcctaagtaa tccacctgct tactaatggc
    33601 tgctatacaa atcgcatcac ccgctttagc ctgctcaaaa atacgacgac catcccattg
    33661 gctaatgtca tccccataag ctaaggccac ctcttgaaca agagctgtgg tagatgccag
    33721 atcctgaaac tgaccatctg acaaatgaag gtaaccaact tcgcaagctg aatgactact
    33781 accatgaaaa acttgggaat taaacaataa acagccaccg attcccgtcc caattgtcaa
    33841 gcaaagagcc acaggataat ccttggcact gccagaaata gcttcggcta gaccagcaca
    33901 gttaacatcg ttttccactt cacacggtaa cccgaaagtc tcttcaatct cgtttttaaa
    33961 ctgagttcca gcatagttag gaatctgagg gccggcataa aagatttctc ccttatcagg
    34021 attaaccatt cctgcagaag aaatggccac tccagccaga tccatttggt cttgataggt
    34081 ctttaccaag cccttgacct tttccaaaat agagggacca cctttataag cctcagtggc
    34141 catttcctct ttttccaaca ggtctcctgt ttcagaaatc aagccatact tgatagcagt
    34201 accaccaata tcaattgcta aataatgctt catttgttat cccctcgcat cttattaaaa
    34261 ggtttcctta gcctgattaa tcagagcagc agcccgttgg caaataaccc tatcttcctc
    34321 gaccaactca gctaaaggtg atcgaacaga accaatatca agaccctcat taatacgaag
    34381 tacttcctta ataaccccat acatattgcc gtgagcagaa acaaggacac cgataatctc
    34441 attaatagta tattgaaagg tcttggcctt ttctaaatcc ttgtcaacta taagttgatt
    34501 cagccttaaa aataattctg gcatagcccc gtaggtacca ccaataccag cagcagcccc
    34561 catcaagcgc cctcctagaa attgttcatc tgggccatta aaaacaatat gatcgtcccc
    34621 gccaatcgca cagaaggttt gaatatcctg cactggcatg gatgaatttt taacgccaat
    34681 cacgcgctta ttagccaaca tagtcttata aaggcttggg gtcaaggcaa cccctgccag
    34741 ttgaggaata ttatagatga taaaatctgt gtgaggagca gccgaagaaa tcgcattcca
    34801 ataatcagca accgcatatt ctggtaaccg gaaataaata ggcggaatag ctgcaatggc
    34861 atccacgccc aaacgttctg aatgagctgc caattcaata ctatctttag tgttgttgca
    34921 ggcaacgtga ttaataatcg tcagcttgcc cttagcgact gccatgacat tttctaacac
    34981 caattgacgg tcaaaaacgc tctggtaaat acactctcca gaagagccat taatgtaaag
    35041 cccctgaacc cctttgtcaa tatagtactg ggtcaaggca cgcacacgct cagggctaat
    35101 agctccttgg tcatcgtaac aagcataaaa ggcaggaata atgccttggt atttcgtaag
    35161 atctgtcata gtaatgtcct ttattcaatg tcattattaa gtgggaatcg cctaagaagg
    35221 gattccatga taatcaagta gaggtaagtt aggctactga tgccgatgat agctagcaaa
    35281 gacacgcctc caaataacgt taacagagac aattgtaggc taaatatcgt gagacaaata
    35341 agagccaaga aagcaaaact ccatggaaaa aagagtccgg ctagtaaaaa ggttcgctta
    35401 aacagcgtag atagggaaag ctcttttctg acggcctgag gataagcgta caaaaagagt
    35461 ataactacca gaaataaaac agcaatgcaa agtactttaa acccttgaaa aactaggcct
    35521 gattggtttc gaattaagaa aagatctaag acacaaatac tcattatcaa aagctcaata
    35581 agacctaggc gcatcccact tttaaaataa tgtttaaaat gagagctata agtagtaaca
    35641 agctgactaa cttgaccttc tcgccaatcc aacaaactcg catacaaagc cattttagca
    35701 accccaatcg ttagaagggg aaggcaggac aagacaaata agagattaaa cacaagcaaa
    35761 tcaaacagtg ctgcactagc cctcatccat ttactatcaa gattaaaaag agaatgtaaa
    35821 agtccctgtt ttttcgacgt caaatcacaa cttcctttcc atcaattggt tcactgccaa
    35881 atcaacctaa tctgccatta acactttgat taagcatttt gtaactgctt cccctgcttg
    35941 gttaaaatta ttgggttgat gaggctcatt aggaaaaaag taagcaaagc tatcatcaac
    36001 caaatctagg tcataggttc gatcacaggt tacaaagcca atatcagctt cttgttcaaa
    36061 aggcactact tcagcttgat tacctatacc ataacgaatg cactcattcc cagctagcaa
    36121 aaggtggcaa tctgcatacc gtttgtggta ttccaacaat gaattgtccg ctttactcaa
    36181 ttgatttctt tgaataactg caaatacttt ctcccctaaa ataggatatt tcccttcagc
    36241 tagttgcctc aaatctgtgg tttgtaaaaa ggtaatcgcc gcatccaaat gagggtgaat
    36301 ccctcggtaa cggctaatat tttttaaacg atcataaatc atgttagaaa cctttcttga
    36361 tttaaccttt cacagcaccc atagtaatgc cttgggtaaa tgatttttgg aagacaagaa
    36421 agaccgttac aataggcact gcagccatgg cagcacctgc cataatcaag ccataattag
    36481 tagccatttc agcttgcata gtagcaacac caagtgaaat cgttaaattt tcccgagagg
    36541 ttaacatcac caactgcata aaataatcat tccaagtatt gataaaggta aagatcgcta
    36601 gcgctgcaaa gcctggtttg acaataggga aagccacatt gaaaaaggtg cgaatctcgc
    36661 cacatccatc aatcttggcg gattccaata actcggttgg aatattttct gagaactgtt
    36721 tcataaggaa aacaccaaat ggccagccca caagaggcaa aataacagcc gccaaagtgt
    36781 catggattcc cataaaatta acaatccgca caagaggcac tagaacaacc tgttttggca
    36841 aggccatagc agcaataaaa atcgaaaaca aaagccgctg tccataaaac cgctttttgg
    36901 ccaaagcata gccagccaaa gaggaagtgc cacaaaccaa gaacatggtc gccaccgaaa
    36961 taaagacact attccacaac cattttaagg ctggattttg cactaccaag gccttaaaat
    37021 tttcaatagt aggtgccttt ggccaccatt gcggtggaat cataatggta tcggcttgag
    37081 ccttgaatgc tcctgtcata atccaataaa atgggaaaat aaacaaaatg gtcaagatgc
    37141 atagaataac agtagtcaga acatccgatg cggttagttt ctttttcttc atcttaatat
    37201 cctcctaata ttccacatca ttacctaaaa ttttaaattg agcaaagctg atgattgcaa
    37261 tcatgactgc caagaataca cccattgtgt tggcatagcc gtattcagag agcttaaagg
    37321 ctttttcata caaatagtac atgagggtgc tggttgaata atttggccct ccagacgtca
    37381 agagttgaat caaagcaaaa cattggaagg aattaattgt tgttatgaca gcaatatata
    37441 aggttgttgg cagcagactt ggccacttga tattccaaaa cacctgaaat tccgtcgccc
    37501 catccacacg cgcagcttct accaaagagt tatcaatatt tcccatagct gcaatatata
    37561 gaataatagg ctgcccaaca gatgtggtta ataaaataac aataatagcc aacaaagccc
    37621 agtgcttatc tcccagccaa ctaatatttt gttcaatcac atgagcatat tttaagacat
    37681 aattcaaaat ccctgacatc ggatcataaa tccatttcca aaccaccgtt acggcaacac
    37741 ttccggtaac aacaggtaga aagaaaactg cccgataaaa cgaacgtgcc accacatttt
    37801 tgtcataagt cttagcagcc acaaagaggg aaaagaaaac tacaacaggt accgaaccaa
    37861 taacaataat caaggtgtta ataagagact tcatgaaaat gggatcttga aacatcctag
    37921 cataattagc aaaaccaaca aaggtaaaat ccgtcatgga ataattgaaa aaactcgtca
    37981 caaagcccat aatcatcggt atcaagacaa aaatcacaaa gaaaaccaag accggagcta
    38041 aaaaagcgta tgagatgagc gtctctctca tttttaattt attgacgttc acttgtaata
    38101 cctctttttc tattaatctc tcctaaaaaa aggaaaggct cctttccagt gaccatttga
    38161 catgtgccgt gaaaagaagc cacttgttct gaacacttat tgtgtttttt tgattgtctt
    38221 gttcgctttt tcagtgaagg ctttcaaagc atcttctggc ttttcatcac cattagagac
    38281 ggcttgaacc attgggaacc aaagagttct catttcagca aacccatcaa tcgtgttgta
    38341 gtatggtgag tagaattttg tccattcagc aatttttttc attcgtttgt ctttgtaaag
    38401 atccccgtaa gaagttctca caggaaaggc acctgtacgg acaacatttt taggtcccca
    38461 ttctttatca tcagcgataa attggataaa tttcttagag gcagcaactt tttgttcatc
    38521 tttgttatta aagaccgcaa aaccatttac gaggtattct agttctggtt tgccatcatc
    38581 tgatgggaat gggatttcaa ggtaatccac tttactagct tctaacaatt tagcttggat
    38641 gcctggttgt gcaggcgccc aaagaatggt aaaggaagtt tggccattcg caaagttttg
    38701 aatatcagct gaaccgtcgt attgagagcc attcatcatt aaaccatctt taatccaatt
    38761 ggaaatcttg gtcatggctt taatagagtt agcatcatct gtggtgtatt tggttacttt
    38821 gtcatcggta atatgactac tataaaggtt agcaaagaag gcacgtggtc cttggtcacc
    38881 accttgacca ttagcaaaga aagaacctgg gttatatcct ttgtccttca atgctttcaa
    38941 gactttttca aaatcgtcag ttgtccaacc ttctttcacc aggtcaagga cgccagcatc
    39001 tttgagcatt tttttattca acgccatgta gaatggtgca gagctaatag gatacatgta
    39061 agctgtatct cctgcttttg aggcttgaat caatttgtca ttgttaacat ctttggtaaa
    39121 ctcttttgtg aacaagtcat tcaagtcagc caatttccca tttttaccat attgaatgat
    39181 acgacctgga gcatcaaaga ggacatcagg agctgttcca gcttcaatag cagttgtgat
    39241 tttctcagga ccagatgtaa aatcaatggt ttccaatttt accttgattt ctggattagc
    39301 tttctcaaag gcagcaatca atttcttttc gtaagttccc acaccatctt cagctttttc
    39361 ttgcgtaaag actgggaaag cccaccatgt gatttctgtt ttagcagcat cagatttact
    39421 ttcactggcc tctttctggc tctttccgcc acaggctgct aatcctaaaa cagatgcacc
    39481 aagcatcgct aatgaagcaa acttcttcat attcatcgta aaatctcctt tttttgatgt
    39541 gtgtttctat tttgacaacc actattagga tttgagcgct tcgataaaac gctctgcaat
    39601 ttccttaggc cttgtaatgg cccctccaat aacaatacct gccacaccta agtcattaat
    39661 tttcttggct tcttctgggg aatgaatctt tccttcggca atgacagcaa tgcctgcttt
    39721 gcaaagagct tctatcaatg ccacatcagg acctgcttct tgacggctat atggggtgta
    39781 gcctgagagg gtcgttccaa caaaatcaat gccagcctga tgggccacta gtccttcatc
    39841 aaaggtacta atatctgcca tcaaaagctg attggggtac ttttccttaa cttgtcgaat
    39901 aaagctagca atatccaagc catcatgtcg gtcacgcttg gtacaatcca tagcaatcac
    39961 tgcaatattt aaggcagcta attggtccac ttcggtcatc gttgccgtga taaaaggctc
    40021 ttgcggcggg taatcttttt tgatgatacc gataattggt aggtctgtta tggcttgaat
    40081 ttctttaata tccctaaccg aattagctct gatgccgaca gcaccagctt cttgagcagc
    40141 ttttgccagc agtggcataa tacccccagt ctctgaataa agaggctcac ctggcaaagc
    40201 ctgacaagaa acgataatct caccctttaa ctgctccatg agcttttctt tggttggttt
    40261 atcaggcata gtttctactc cttgtgtttc ttagttttaa taaaaataac gttcacaaag
    40321 ttattataac aaaaaataaa gcgctttcaa tagcgatgag acttttagaa tctactttca
    40381 ttttttatat tattttctat tatttggaat gctctttcat acaacaaata aggaggtaag
    40441 gagttgacac catacttata cattgcaaat ttccgattag aaaacgttaa aaaaccaaga
    40501 gcctcgagca ctcttggttt actttagttg tattttttgc aattaagctg ataatacttt
    40561 acgtccttta cgacgacgtg cagcaagcac acgacgtccg tttttagttg acatacggtg
    40621 acggaatccg tgtttacgtt gacgacggat tttacttggt tgataagtac gtttcacagt
    40681 gaatacctcc tcatagattt tgtattcgtt tagccggcta tttgtgatat cagttactaa
    40741 acatactata atattctatc ttaaagaggg tctactgtca agatttattt tcgtttttca
    40801 acagtttctc tctcatttaa gaggccgctc atttcataaa gtgtctcttc tttgacactg
    40861 ttcctttgcc ttaccgttga aaccttatca atagcaacaa taatcaatga gatcagtaaa
    40921 acaaacggtt aagctggcca tacaaaaaat cttcgatagc taaactaact accaacgatt
    40981 ctttttagga tctaacgttg aagactgact accacatagc ggttagggtc attgccttca
    41041 gaataactat caaccccttc aatggatgaa acggttttat gaacgatttt acgctcactg
    41101 ttactcatgg gatccatggt gtaatcttgg ccagattcca agacgcgttt ggcaactttt
    41161 tgcgtaaagt caatcaaggt ttctgttcga tgttccacat aatcatggac attcaaggat
    41221 actgaaaaat tttttgaata gcggtcgtgc aagaaatttt gagctaacaa ctgcaaggat
    41281 ttcaagacct taccatggta accaatcaca cgtcccgcct ctggcgtttc aatctgcaaa
    41341 ttgatttggc gacgattgtt gctggtttca acggtggctt cgatatccat ttcatagatg
    41401 attttagtca cataatcgga cacttcctga gcagcttttt caatatcttt gctgaaacca
    41461 tcattaccag gatcttgggg cacttctgat tcctcgactg ggattgacgg ttgttgtgct
    41521 tttctttcag tgactgtctc agcaagatta gaaggggttt gcgctggact atcttcagac
    41581 atgagaggaa ccactttggt atcttctgct tctaaccttt gagtggcttt tatttcttca
    41641 ggttctacat cagcagaatt gaccacggga gcattttttt gattaatatc ctctggaaca
    41701 cctcgagtgg cttttttatc agccttataa accgtcttgt cgctgatacc ttcgatatca
    41761 acttgggctg gctttttacc aaacccgagg aaaccttttt tctcttttga aatgactttg
    41821 atatgcgctt taaggcgtga taaccctaac tcttgaagtc ctgtttcgat agcttcttcg
    41881 actgtttttc ctgtaaataa taccataacc taaactccta ttttctcttc attgcttttt
    41941 tacgagcacg acgttcacga agacggcgtt ctttttcttc attggcaaga cgctgtcttt
    42001 cggcaataat cttaaaagga ttgtttaata acagtagttg taacacttgg aaggcattgg
    42061 agaccgtcca atagagcact actccactag ccaagttaaa gcccatgaaa atgatcatta
    42121 gcggcatcac ataaatcata acagtcatca tgacattttt ttctttagcc gctagattgg
    42181 tcaaccaagt ggacaagaaa gtgaagacag ctgccaaaac tggtaaaagg tacaaatgat
    42241 catgttgtgc caattccacc cacaagaaag tccctgtttt taagaaagat acccgtgtca
    42301 aggcttggaa tagggcaatc ataactggca tctgaattaa gaggggtaag agactggcat
    42361 aagggttaac cccatatttt ttgtaaaggg cttgactttc ttcagccaac ttcatccgcg
    42421 tttgtgtgtc tttaccagcg tatttctttt gcaattctct aagctcaggt tggatgtctt
    42481 gcatcttctg gcttgatttg atttgcatat taaacaaagg catgagcatg agacggatag
    42541 taagggtaaa aagaataatg ccaacaccaa ttgaaccatc aaaggaaagc cattgaattg
    42601 ctctggcaaa taagtaaacc aactggtccc aaccactaga tgattgtgcc gttacctcac
    42661 cacgaccaca ggccactaat aatagaacta aggggactat tcttgcaatt ttaatgtttt
    42721 tcttcactct caaaaccttt ctcaagcaat tgtgctaact ttaaaacatg atgtaaattt
    42781 tgttgaagct cttgatactc caaagactcg acacccttac gggcaatcac cacgaaatcc
    42841 tctgacttca gctgatgccc taatgccatg ataacatgac gtatctttcg tttgactgca
    42901 tttctggtga ctgcatttcc tattttttta ccgacagaaa tacccacacg gaagtggtct
    42961 tggcctctat ttaaatgata aatgacaaat tttcgatttg ctgtactttt tccatcctta
    43021 aatatggctt ggaaatcttt ctcacgcttg acacgatagg tcttcttcaa aatttaactc
    43081 caatatctaa attattacca ttataccaca tcccctaaaa aattaaaaag ccctaaccct
    43141 ttcagattaa gcctctttct gtactatttc tagtttttgt gcttcaactg cctcttgtca
    43201 ctccatcttt tcgtagtata gttcttcttt cgacaaagga ataaccttca gaaaacaacc
    43261 ataaaaagtc agaatccaca agacgtcagc tacttatccc tgtatttacc tatcaaaaaa
    43321 cccggcaagc cgggctttaa tactgatttt acaaccagct aatgtgatat ccaaacaaag
    43381 acaactgaca tcgtcatttt gtctgccgag ccatcatatc aaaaatacta tttgtttatg
    43441 gagagagaga aaacaatatt ttgattgaat tggggattga ttgctcgata aactcatgac
    43501 ttcgtaaatc tttatttaca ttaattatta tagcgaaaaa tacttcaata agagcatcat
    43561 ttcctgaaat gttgcttttt cgtcctaaaa tgttattgca ctcttaactt tagcgaaggg
    43621 cctcccaccg ttcaaacaga catttcattt ttaaccgtga taccatacaa gaatcaccat
    43681 tttcgaaata aaggagctgc ttactcttat caattcgaac aaccttatct agattcacaa
    43741 ggtaggatct atggcacaaa aaaagtttcg gtgctactgc ttgaatctca gatagattac
    43801 cataaaactc taatctttct gtctgagtcc ataaacaaac tttgtgagga gttgtggctg
    43861 tagcaaagta taaaatatcc ttataaggca attttaatct tgtctgtgga gtctcaaata
    43921 aaaacatgtc ctttgactca cgactagaca tcatatcgta agtatatcgg atacattctt
    43981 cgatttgttc tttaaactgt ttttgaccac ctgctttatc aataaaatct agagcagata
    44041 ccttatattt aaaagaaata ggagcaaatt ctgaatgagt ggtgacaaac acaataacgg
    44101 cattaggatc tttttgtcga atagctgcag ctaattcaag cccacaacga gtatattcac
    44161 caatttcaat atctaaaaaa tacagctgat ggtctcctct ttcttggata ctctcaaata
    44221 atttttgagg agtcgagaac acttcaagct gattacatgg tattctagtc tcttttaaga
    44281 tgcctactac gatagattct atgcgtgtct gctggataaa atcatcctca agaataaaaa
    44341 tgttcatcat actttatctc atttctaaca attgtcttaa tcgataatgg tcacttttag
    44401 tcgaaagcgt tatttggggg taactatcta aaacagtcaa aaagtgggct gagttctgct
    44461 gctgagcatc aaagcgtttg gctatatcca cacgattggt ttttgtgcta ttttcaataa
    44521 taaataattg cttgttatct tgtttaaagt atgcaataga taaaaaagga cgtctactgc
    44581 ctttggctgt ctcaatagca ttgtccacta gtccagtaaa aagtacaatt aagtcgatta
    44641 acttaatagg taaatgatcg ataacatctg gaatctcaat ataaagttca atacccaaat
    44701 actgagcttc aagcaatttg gaattcatga tgctgcgaat cacagtctct ttgatattgt
    44761 ctaaagaaga tatttcataa tttgtgcgca tcatagaagt tgccgattgc tgaataaccg
    44821 tgtcataaac tctagtaatc agatctatat caccactatc gatactatct tttagactaa
    44881 ttaaaatatt ttcagaatca tgtttaactg ttctgatttc tcggtaaagt tgctcaatcg
    44941 attgattgta attttcaaga tacgcaatac gttcattttg tgcttgtgtt aatttttgtg
    45001 acagctgatc tttagcaaat cgatctaatt tcacaataac ccaaataaaa atagctaaat
    45061 aaataaatac taaaatagac cggtactgga aatatagtgc aagaaatccg ctttgcacag
    45121 ttaccaaaaa atgggcgaaa aagtagtaag aaaacatggc agtattcatc caaaataccc
    45181 actttttcat cttatcttca ctgataaacc ttattaagct taagtcaata ctgaaaatgt
    45241 agctaaacag ataaaatatt ggtaatacaa aaagataaca taactctaaa agcttaaggt
    45301 tattattaat ttgaccaatg ctaaatccta agacagctgg taaaaagaaa gatccgatga
    45361 cgcgaaacag taattcgaca atcaagatag taaagaaacc attaaacata tgctcaaata
    45421 atttcttttt cggagcactt aacaaactaa caataataaa aaacagttgg tcaatcaaaa
    45481 tgactttgtc aaatatcata ttagcaatta caaaactaat gcctataacg ctaacccgtt
    45541 tccagcttag tttaatagca gaaacctttg caaaaatgac ccagctgtta ataaagatgg
    45601 tagcatagaa taaagccata ctgtatataa ccatcatcta tccacctcct cctttggcat
    45661 ggtcaaggtt tggctaaatt ccttatcaaa acttttagtc cgcaaggata aataaggata
    45721 ctttttgaga atacgctgcg cattagaaag gccgatacca cgttctgaac cttttgaaga
    45781 atagccttct tcaaagatct tgttaatgtc tacctttttt ttagtcgtat ttgtcacaac
    45841 aaacatctga tagtcatcta acaaaaagta agcaattgac atatgaggtt gctgtgcttc
    45901 aagagctgcc tcaatggcgt tatcacaaaa aatagacatc aacaatagta agtccaactc
    45961 aggaatgtaa gtctctttta tattatctgg cacttccaca ttcaaatcaa tacctgattt
    46021 ttccgcactg ataattttag ctgacaataa gctcttaatc gatgagatat taattttcct
    46081 aagcttcgag atattatagt gtttatcatc ccagtagtcg ttagcttcat gaaccgtttg
    46141 ggcataaata tcttgaatct gagtaatgga ttcactctta atggcttgac ctaaacgctc
    46201 tatacggctc aggtaatcac tttgaaaagc tcttacatct cgatagagtt tctcaagatg
    46261 tttcccataa gtctctaagt tagtcatata agcttgttgt tctcttctca actcattttt
    46321 aaggtattct ttagaactat gattaaaata agatagaaga taaactaaaa gaagtataaa
    46381 catcaaaaac aataacttat aaggtgtggg aacaaaaaga gaatgatgaa aatgtaaaaa
    46441 ggggtccgta aatcctaaaa tgacaaaaat gtccacataa taaaccaaaa caaaaatcag
    46501 caatgtttga tagaaattat gcgatcgttt cgaatatccg gattggcata tcaccttgta
    46561 atcttttcca atcatataat taataatagc aaaacttgga aaaatcagca gataagcaag
    46621 caagtcaaag ataatatgac ccagataaat accactcgac aaaaaaagat aggggataac
    46681 gattgtaccc ataaatcgag atagcaaatc caccaaaaca ctaggtgcaa cagccaaaaa
    46741 aagagctttc aaagaaaata attgcttggt ttcttgcctt aaaacaacta aaaataatag
    46801 aggatcaatt agcaatggcg acaaatacgg gaactg
//
LOCUS       DAUCWO010000007        44188 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUCWO010000007 DAUCWO010000000
VERSION     DAUCWO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44188
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.007"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            116..337
                     /locus_tag="VGY20_000367"
     CDS             116..337
                     /locus_tag="VGY20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273255.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(417..2699)
                     /locus_tag="VGY20_000368"
     CDS             complement(417..2699)
                     /locus_tag="VGY20_000368"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ4273256.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRNKITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIVNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALVDILLEEDLLINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFRENSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(2721..3380)
                     /locus_tag="VGY20_000369"
     CDS             complement(2721..3380)
                     /locus_tag="VGY20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ4273257.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            3546..4742
                     /locus_tag="VGY20_000370"
     CDS             3546..4742
                     /locus_tag="VGY20_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4273258.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPACKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTAVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            5214..6062
                     /locus_tag="VGY20_000371"
     CDS             5214..6062
                     /locus_tag="VGY20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ4273259.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(6397..7293)
                     /locus_tag="VGY20_000372"
     CDS             complement(6397..7293)
                     /locus_tag="VGY20_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ4273260.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            7591..8343
                     /locus_tag="VGY20_000373"
     CDS             7591..8343
                     /locus_tag="VGY20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ4273261.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDTLFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            8328..9277
                     /locus_tag="VGY20_000374"
                     /pseudo
     CDS             8328..9277
                     /locus_tag="VGY20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
     gene            complement(9457..11784)
                     /gene="pflB"
                     /locus_tag="VGY20_000375"
     CDS             complement(9457..11784)
                     /gene="pflB"
                     /locus_tag="VGY20_000375"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ4273262.1"
                     /translation="MATVKTNTDVFEKAWESFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            11994..13088
                     /gene="dinB"
                     /locus_tag="VGY20_000376"
     CDS             11994..13088
                     /gene="dinB"
                     /locus_tag="VGY20_000376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ4273263.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVVGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDLVEPMSIDEAYLDVTDNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(13181..13663)
                     /locus_tag="VGY20_000377"
     CDS             complement(13181..13663)
                     /locus_tag="VGY20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273264.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(13754..16207)
                     /locus_tag="VGY20_000378"
     CDS             complement(13754..16207)
                     /locus_tag="VGY20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ4273265.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMTDALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNANPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(16265..16858)
                     /gene="lepB"
                     /locus_tag="VGY20_000379"
     CDS             complement(16265..16858)
                     /gene="lepB"
                     /locus_tag="VGY20_000379"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4273266.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(16869..17771)
                     /locus_tag="VGY20_000380"
     CDS             complement(16869..17771)
                     /locus_tag="VGY20_000380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ4273267.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            17928..18236
                     /locus_tag="VGY20_000381"
     CDS             17928..18236
                     /locus_tag="VGY20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273268.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            18239..18784
                     /locus_tag="VGY20_000382"
     CDS             18239..18784
                     /locus_tag="VGY20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ4273269.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            18933..21272
                     /locus_tag="VGY20_000383"
     CDS             18933..21272
                     /locus_tag="VGY20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ4273270.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYAPQNAGGSGATIVTLG"
     gene            21276..21776
                     /locus_tag="VGY20_000384"
     CDS             21276..21776
                     /locus_tag="VGY20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ4273271.1"
                     /translation="MESYEHFYAKLSQPFRKSPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            21857..22171
                     /gene="trxA"
                     /locus_tag="VGY20_000385"
     CDS             21857..22171
                     /gene="trxA"
                     /locus_tag="VGY20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ4273272.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQTPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(22223..22810)
                     /locus_tag="VGY20_000386"
     CDS             complement(22223..22810)
                     /locus_tag="VGY20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273273.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKRDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSSGKLLDEIEKREE
                     RAKLPYQNPLIVTVFFLITVAIAFASRFMFTWLFH"
     gene            complement(22987..24111)
                     /gene="mutY"
                     /locus_tag="VGY20_000387"
     CDS             complement(22987..24111)
                     /gene="mutY"
                     /locus_tag="VGY20_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ4273274.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNT"
     gene            24309..24602
                     /locus_tag="VGY20_000388"
     CDS             24309..24602
                     /locus_tag="VGY20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273275.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            24775..25065
                     /locus_tag="VGY20_000389"
     CDS             24775..25065
                     /locus_tag="VGY20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ4273276.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            25087..25578
                     /locus_tag="VGY20_000390"
     CDS             25087..25578
                     /locus_tag="VGY20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ4273277.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            25743..25982
                     /locus_tag="VGY20_000391"
     CDS             25743..25982
                     /locus_tag="VGY20_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ4273278.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(26115..26771)
                     /locus_tag="VGY20_000392"
     CDS             complement(26115..26771)
                     /locus_tag="VGY20_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ4273279.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(26904..27848)
                     /locus_tag="VGY20_000393"
     CDS             complement(26904..27848)
                     /locus_tag="VGY20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ4273280.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            28051..30879
                     /gene="uvrA"
                     /locus_tag="VGY20_000394"
     CDS             28051..30879
                     /gene="uvrA"
                     /locus_tag="VGY20_000394"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ4273281.1"
                     /translation="MQNKIIINGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPVVRRKK
                     GQHKTVFEKIQKDGYVRVRVDGDIFDVTGVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLKEGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            30995..32068
                     /locus_tag="VGY20_000395"
     CDS             30995..32068
                     /locus_tag="VGY20_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ4273282.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLGADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDVSEIETIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIAASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFLGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            32103..32564
                     /locus_tag="VGY20_000396"
     CDS             32103..32564
                     /locus_tag="VGY20_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ4273283.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            32660..33217
                     /gene="efp"
                     /locus_tag="VGY20_000397"
     CDS             32660..33217
                     /gene="efp"
                     /locus_tag="VGY20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ4273284.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            33263..33652
                     /locus_tag="VGY20_000398"
     CDS             33263..33652
                     /locus_tag="VGY20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4273285.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            33645..34097
                     /gene="nusB"
                     /locus_tag="VGY20_000399"
     CDS             33645..34097
                     /gene="nusB"
                     /locus_tag="VGY20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ4273286.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(34238..35203)
                     /locus_tag="VGY20_000400"
     CDS             complement(34238..35203)
                     /locus_tag="VGY20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4273287.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEILKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(35208..36647)
                     /locus_tag="VGY20_000401"
     CDS             complement(35208..36647)
                     /locus_tag="VGY20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ4273288.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRNANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYNYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            36889..38772
                     /locus_tag="VGY20_000402"
     CDS             36889..38772
                     /locus_tag="VGY20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ4273289.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYGEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSPKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGLAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            38949..41927
                     /locus_tag="VGY20_000403"
     CDS             38949..41927
                     /locus_tag="VGY20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ4273290.1"
                     /translation="MVYQLGSHDLVPKIRSVQMDKHLLVKRTLGCVCAATLMGAALAT
                     HHDSLNTVKAEEKTVQVQKELSSIDSLHYLSENSKKEFKEELSKEKVPEKVKEILAKA
                     QQADKQAQELTKMKIPEKIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKE
                     VDLAFIFHDWTKDYSLFWKELATKHVPKLNKQGTRVIRTIPWRFLTGGDNSGIAEDTS
                     KYPNTPEGNKALAKAIVDEYVYKYNLDGLDVDVEHDSIPKVDKKEDTAGVERSIQVFE
                     EIGKLIGPKGADKSRLFIMDSTYMADKNPLIERGAPYINLLLVQVYGSQGEKGGWEPV
                     SNRPEKTMEERWQGYSKYIRPEQYMIGFSFYEERAGSGNLWYDINVEDESNPNIGKEI
                     KGTRAERYAKWQPKTGGVKGGIFSYAVDRDGVAHPKKNGYKNPKLDNIVTSDYSVSKA
                     LKTVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEG
                     LNKFKKLAQLDLIGLSRITKLDRSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPV
                     SLKVSGLTGLKELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDT
                     MLSTVSNHAGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVAEGNIDLQSQLLFGTVTN
                     QGTLINSEADYKAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTL
                     ATDKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAAGATVIKSENDENAKKVF
                     NGIMEYNPLSFNNKSSIIFEMKDPSLAKYWRLFNDSSKGKDDYIKEAKLEVFTGQLNA
                     EADVKTSLEKSDDWQTVSTYSGQEQVFSHALDNISAKYWRITVDNKKNQYGCVSLPEL
                     QILGYPLPNADTIMKTVTAAKELSQQKDKFSQKMLDELKIKEMALETSLNSKIFDVTA
                     INANAGVLKDCIEKRQLLKK"
     gene            42191..43075
                     /locus_tag="VGY20_000404"
     CDS             42191..43075
                     /locus_tag="VGY20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4273291.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     LHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQAIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVVDN
                     GSATLGNFALAKLAAQGK"
     gene            43170..44123
                     /gene="manA"
                     /locus_tag="VGY20_000405"
     CDS             43170..44123
                     /gene="manA"
                     /locus_tag="VGY20_000405"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ4273292.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GHEHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
BASE COUNT        13411 a         7811 c         9317 g        13649 t
ORIGIN      
        1 aaggtatcgt aaaagatggt ctaaaaactc tctaaaaact tgacttcata aaaaccgaag
       61 tttataataa acttaacatg attaactaat ctcattttag tgtggaggga aaattgtgac
      121 caaatcaact ttaaaagagt taagaacaac aaagaaaatg acacagcagg aattgtctca
      181 cttaacaggt atttctgtca gaacgattgc taggtatgaa aaagatacta aaaagctaag
      241 gcgagcaaaa tatgagaagc taaaaggtat tgcacaagca ttagcggttt ccgttgatga
      301 tattttttta ggaatagatt cggaatttat gaactaagac gatcgttttc tgtttgctat
      361 tagtaagacc aattaacaaa aaagtgctta gttatattgc taagcacttt tctttgttaa
      421 tttgatgcta ttggcacaat caattgagac tgactgagat cgatagttaa agcatagttg
      481 ctgttgtcac ggacagtatg ttcaaaatcg gttgtgtata agatgactcg aagggtatct
      541 cctttttcta gatgatagat ggttggttgc aaaggaagtc taacagtcat ccatttatta
      601 ttaggaatag tttcaattga cgacaaatta ttgcgatttt gtaaattcat gaaacctttc
      661 gaaataacac ggtaagaatt ttctctaaat ggtagctctt taaggggttc tctagagaaa
      721 ttttgcccat tgtcaatact tgattgggtg agagcaatag gaagatcgcc caagcgtttt
      781 ttcttaccat agtcaaggat ttgtgcagat agaagtcctt tattttcact tgatttcact
      841 tttaactgta ggacaatttc accgttaata agtaagtcct cttccagtaa aatatcaacc
      901 agggcttgat tggccttgcc tttaaagaga gctgctttga acgcacggaa atctttaccg
      961 taagctttaa attcatcttc accatagtga ttatcaatta aaagcagttc ctgaccgagg
     1021 tcaagctctt tgatggttga ggttccaaag actttacgct cctgccagtt ttgtgggcag
     1081 gtgttatcct gccagatcat ttcaggaagg ctaaaatcat tagctaggcc taagagtttt
     1141 tgacataaaa gggcattcat actttcgcga aaatcaatgg attgccaatt atgcatgtaa
     1201 acatgctcac cttgatgtaa aaagaggtgc ttgttgattg tggaaggaag cgcattgaca
     1261 atctcataga cctgtcttgg tttgacattc cagtcttgta agccatgagt ataaacgaca
     1321 tcacacttta tttgatgagc attttttaag taattgcggt cgtgccaaaa ttgattataa
     1381 tcaccagatt gtcggtctaa cgcctgagac tgttggttta gcaattcttg ataacgatca
     1441 ttatggcgaa gataatcgcc agccaataaa ttacgcgagt aggtgagctc agttaggaca
     1501 tctagatcct ctccaggata gccaccagga ctacaaacta agccattttc gcggtagtag
     1561 ttgtaccaag aagaaatagc tgattctgca ataatcatgg ctaatccgtc tacgcctgta
     1621 gtggctaaac cagtagacat agttcccaag taggactttc cagtcgtaca aactagacca
     1681 tttgcccaat cagctctcac ttggtgggtt tttgagtggc tggtgtaggc tgtggctctt
     1741 ccatttagcc aatcaataac ggctttaaag ctttcaattt gagcatagtc tccactcgtc
     1801 ataaagccag tggaaccagc agttcccaca cctgaaacat agatattagc aaacccacgt
     1861 gcgaggaaat aatcgttgag gctataggag ttaatgtagc taaagctttc tagattttga
     1921 cctataggaa gtttgcaagg ctgtgtctca agaggaataa aatccctgtc ttcgacagtg
     1981 attttattcc tttttttaac gactaactct ttttccatac gataaagttt tttgtcgttg
     2041 gctacttcat tgataccttg atggtatgga ctaggtgtca tcaaagttgg taatggtttt
     2101 tgagattgag gacgaatgat attaacctta ataagatcaa gttggccatc ttgatcggta
     2161 tcaagaggtg cttcaacgta aacaacttct cgaatcagct gtgacgtgtc aaaggtagct
     2221 aatgatttac cgttgaagaa gtggtaatca ttgtccatag taagcattcc ttggctgacc
     2281 aaatcatcaa tcagggtcat gccagactta caacgggtcg ctaataagtg gtggatatgc
     2341 ttgataattt ctgtttcgtc aaagtggata ggaaaggcca gtttatcaag gaaagcatcg
     2401 gcctctgtaa aatcaactcc aggaataaag ccaaggactt gcagggccag taaatcaaaa
     2461 acctctttgg ataactcttg ctctgattga aaaaagctca atgcgtccat gtcatttttg
     2521 gcgattagat gacttaaagg ataatcactg tcagggtagt ggaagaagag tttacgtaaa
     2581 aagctttcta ggttagcttt ggcagttttt tgcagatcca gatcaaagcc caattctttt
     2641 aattcttcag ccgctctttc tagactagtt ggaatatagg aaaattgatt atagcgcata
     2701 ggagtttcct ttctagtggc ttagaaaacg atattatctt ttaatttgtc aagattggta
     2761 atgataatat gttgtttatc aatgcgaatg atgttttttt ctttcaactg ttttagaata
     2821 cggctgacgc tgctagcggt agaaatgcca cagaatttac cgagttcctc attagtaatg
     2881 acaaaattaa ttaaaatttg gccatttggt agttcttcgc caaatagggt cattaagtca
     2941 tatatttgag tagaaatggc accgatacgg ccgttagtta acatacattg catttttttc
     3001 atggattttt gaaggcgatt atggtaaaag tccttcacat atccttgtag ttcaatatct
     3061 tcgtttatat cttttaaaaa tgctgagcgg cgaaccttgt aaaaactagc tttctctgac
     3121 tcgatgcgaa cattataagg ctctcccata tcttttgaat aaccagtgtt gagaacagaa
     3181 gtaatttcaa gtcctgtaac atatctcaaa ttaaattcca ttccatattt tgaaagaaca
     3241 ctctgcttca caatgccgtc ttttaaaata taagtgaagt cctcttcgag actttcaaaa
     3301 gtcaagtact tgtgataact tttttcaatg ataggaaagt tatgattatc aatataccgt
     3361 tgcaaaatgt gatgattcac gcttttctcc aaatattctt ttaccaatgt ataatttgta
     3421 tcttcaagag aaattaacat ttgctaatgc cctttgatag taacacttat attataccat
     3481 tgaaatatat tatattctgc tttttttgta aaatgtttat gaaaatagag aggagttcat
     3541 tttcaatgga agaaaaattg tttaacaaac attttgtggc catcacagta ataaacttta
     3601 tagtctatat ggtctactat ctttttacgg ttattattgc ctttgtggcg actagagagc
     3661 taggagctca gactagccag gcaggattgg caacagggat ttatattcta gggactttat
     3721 tagcccgttt gatttttgga aagcagttgg aggtgtttgg tcgtcgtttg gttttaagag
     3781 gaggtgctat tttttacctc ttaacgacct tagcctactt ttatatgcca acgattagca
     3841 tgatgtactt ggttcgtttc ttgaacggat ttggttatgg tgtagtatca actgcgacaa
     3901 ataccattgt aacagcctat attccagctt gtaaaagagg agaaggaatt aatttttatg
     3961 gcttgtcaac tagtctagca gcagctattg gcccttttgt aggaaccttt atgttggata
     4021 atcttcatat tgattttcgc atgattattg tcttatgtag cgtcttgatt gggtgtgtgg
     4081 ttgttggtgc ttttgctttt ccagtcaaaa acatgtcttt aaatgcggag caattggcta
     4141 aaaccaaatc gtggactgtg gatagtttca ttgagaaaaa agccttgttt atcactgcaa
     4201 tcgccttttt aatggggatt gcctatgctt cagttttagg atttcaaaaa ttatacacgt
     4261 ctgaaattca tttgacagca gtaggtgctt atttctttgt ggtctacgcc ttaatcatta
     4321 ccatcactcg tccagcaatg ggacgtttaa tggatgctaa aggcgataaa tgggtcttgt
     4381 acccaagtta tcttttttta gcaatgggac tcttcttgct aggtagtgta tcgtctggag
     4441 gaagctattt gctttcagga gccttgattg gctttggtta tggcactttt atgtcttgtg
     4501 gtcaagccgc ctccatacaa ggagtggatg agcatcgctt taatacggct atgtctactt
     4561 atatgattgg tcttgattta ggtttaggtg ctgggcctta cctcttgggc ttgattaaag
     4621 atttggcact tggtagtggt gtggcttcct ttaggcactt attctggtta gctgctgtga
     4681 ttccactgat ttgtaccttg ctttacttgt taaaaacaaa gacaagacag gttgtgtctt
     4741 aaggacaaga aaaggagttt agtcttatac ttaaatacaa acgtatctta acagctgtag
     4801 atagttccta tgagccggga ttagctgtgg gtaaagccgt tactgtagcg ctttgaaata
     4861 atcctagttt gctattggta catgtcaata aaaatgactc gcaaaagcca ttcgtgaaag
     4921 agaaaatgtg gccttatttt tagtaggtgc aattggatta acatcagaat ataggatttg
     4981 tcatgttaag attgatctgt tgatagtgag agctaataaa aaatgttata atactatgaa
     5041 ataaagtgat aaagataagg tcttttatgg tccttttctt taccgctttt attattgtgt
     5101 gaaaataaca cttaatgatc attttattta ttattttatt acctttacat tcatcaataa
     5161 gcctgttaaa atggtatttg ttatatttta ttatttggag gaaaccagaa aacatggaaa
     5221 tgacatggac tgtgaaatac atcacagaat ttatcgcaac ggcttttttg attattttgg
     5281 ggaatggagc tgttgctaac gtcgacttaa aaggaacaaa aggacataac tcaggttggt
     5341 tagtgattgc ttttggctat ggcttaggag ttatgatgcc agctttgatg tttggtaatg
     5401 tatctggaaa ccatattaat cctgctttca cagttggact agcagtgtca ggcttgttcc
     5461 catgggctca cgtcttacaa tacgttgtgg cacaattact tggagctatc tttggtcagt
     5521 tggtagtggt aatggtttat aaaccttact ttatgaaaac tgaaaatcca aatcatgttt
     5581 taggctcatt ctcaacgatt tcatctcttg ataatggtca aaaagacagc cataaagtct
     5641 cttatatcaa tggtttctta aacgaatttg taggttcatt tgttcttttc tttggtgctt
     5701 tggctttgac taaaaactac tttggggtag agttagttgg taaattgatt gaagcaggtt
     5761 acgaccaaac aactgcagca acacaaattt caccatacgt gacaggctca ttggcggttg
     5821 ctcacattgg tattggtttc ttagttatgg tcttggttac ttcattaggt ggaccaactg
     5881 ggccagcact aaacccagcg cgtgattttg ggcctcgttt gttacaccac ttcttaccaa
     5941 aatcagttct tggtcaagcc aaaggtgatt caaaatggtg gtatgcatgg gttcctgtag
     6001 ttgcacctat cttagcggcg atagtagccg tagcagcgtt taaatacctt tatatcagat
     6061 aaaaaaagaa ctaaagcttc ttttagactc aagacaagct cgcttaagga acttgtcttt
     6121 ttatatgagt taagcgaaat ctaatgcaaa cttgactgtc ttgtatagcg tattttgtaa
     6181 attttactag acttcttgta aaaaagcctc tcctcagata agatgactaa tgcctctaaa
     6241 cggtgttaca aataaacgag gcatccctaa cttcctttat ctggtgttga aactattttg
     6301 cacggactaa atcactgata gagtaaaaaa cgatttttca tttttgttgt aaaatattaa
     6361 aaacgtctct ggttagtgat tcccagaaac gttatcttat ctatttgttt gttgcttatg
     6421 agaggtaaag gaggaacgaa tcagcattcc accagtgaag aaaacgatag cgatgaccag
     6481 aagtttcaag ctattaatat ctaaattcgt taagaaaaac acagctagta aaacgccaat
     6541 aataccacca gaaacaattc cagcaaaacc atcccaactg acacggtttg ctttgataaa
     6601 ctggctacta gaagctgtca taatcatagc tgcatctagc atcataacag gcatagcaac
     6661 tgttgggcta agacccatta atgaaaagaa aatcaattct ggagcataat tgcctaaccc
     6721 catagtcatc aagactccaa cgataaaatt aaaaccaatt ccaacaaaaa gccagatgcc
     6781 atggaggcca tgaatggtgt ctgaaaggtg ttctcctgga tggtagatta ttctaatgat
     6841 catgattata gctgctatga ttaaaagact accaaggatt cgttggacag ttggagcatg
     6901 ccagtttttt gtaatatgag tccccaaata agctccaata aaagctgctg cagccatagc
     6961 aagtaaagtt agaacctcaa cttttacgac aaaaataaag caaagagatt gaatgagcac
     7021 cggaagcaca tgagctacgg tcatagtgcc aggcaattgt ctatcgtcag tgactaattt
     7081 agttaactta aagcacgttg tggtagtagc aaaagaccct atcccaaggg tgtcaagggc
     7141 atctgttagc aggccaatcc aaaaccccgt ccaaaagcgt ttaaaaggat tgatcttatt
     7201 ttttttaaga tgttttacta acataaagaa aataaaaagc attgccgaaa ctagcaaagc
     7261 ttgtatcagt ctaaggataa gcatttcgtt cataaagact agtttacaaa aaaaaacctt
     7321 agccgtcaac gtgatttact tcattaaaac atgccactat gcttagaaga gctttggttc
     7381 gatgtatttt gttaaaatta agctaaaata ttaatattgc atgaaagggg acgccttcct
     7441 ttttccctta ctgagcttag ctgttattgt agtgaatgtt taaaaggcta gtttgtcgct
     7501 ctttatcaaa aatctagtag gatgaatacg tataatttct tgtcaaatag aggaattttg
     7561 tctataatag cagaggatta ggaggaaatt atgactttaa tggaaaatat tacctttaaa
     7621 actccagtat tacgcgttaa tgatcgcgac cttaatattg cattttatca aaataattta
     7681 gggcttcgtt tagtatctga agaaaatgcg attgctatct tttcatcttg gggagaaggg
     7741 caggaatgtt ttgtaattga agagtcacct tcagttagaa cgcgtgcggt agaaggccct
     7801 aaaaaggtta atacgattgt catcaaaaca aatcaaccta aagaaatcga acaattatta
     7861 gctcatggag cacattatga tactcttttt aaaggtcaaa atggctatgc ctttgagact
     7921 atttccccag aaggagaccg attcttgttg catgctgaac aagatattaa acatctccaa
     7981 ggtactgatt taccatcgct tgaaaaagat gcgactttta aaggtttaac tcaatttaag
     8041 tttgatatta ttgttttaaa tgttattagt gaagaacgct caaaagcttt ttatcgggat
     8101 ctgtttagtg atcagttacc tattactatg gattttattc aagaagaagg tcccgatttg
     8161 gcaattgatc cccatattgc ttgggattta gaaattttag aatttcaagt ctcaaaggac
     8221 tatgatatga aggtcctaaa agcaacttta gaagaagacg ggcacaaggt ctacattgat
     8281 aaaaaacaca aagtattggt cctttctgat ccaagtcaga tcgaagtatg gtttacgaaa
     8341 tgactttggc agtcattaaa tgtattgaaa accatcttca caaaaaagta tacaaagggg
     8401 ccagtctggc tctctttcaa tctggtcgat ggcaagaata ccatatcgga acaattgatg
     8461 gaagacgacc agttgatgct aacttagttt atgacttggc tagtgtttca aaagttgttg
     8521 gtgtggccac aatatgcaat atttattgaa taatggtacg ttagcattag atgatccctt
     8581 aaaagtatat tatcctagta ttgctgatac gactgtcact attcgacagt tattgactca
     8641 taccagtggt cttgatcctt atattcctaa cagagatgtt ttaaatgcac aacaattaag
     8701 aaaagtactt aatcatctta ctcaaaaaga aaataaaaac ttttattaca cagatgttaa
     8761 tttcctcttg ttaggcttta tgttagaaga gctctttagt gaatctttag accagatatt
     8821 tgataagact atctttactc cctttgggat gtatcatacg tcttttggcc ctcgtccaga
     8881 agctgtacca acattaaagg gtgtgagtga tggagaagtt catgatccca aagcaaaaat
     8941 cttaaaaaaa cattctggat ctgcggggct attttcaact cttgcagatt tagaaagttt
     9001 tagcaatcat tatcttaatg atccattttc agattgtctg tggcgcaact acagccaaca
     9061 aacaattgag cggtcactag ggtggaattt agatggcgat tggattagtc atacaggtta
     9121 cacagggccg tttctaatgc ttaataaaaa agagcaaacg gcagctattt ttttgaccaa
     9181 ccgtacttac gacgaggatg ataaaagtaa atggttaaaa gaacgtcagt tgctttacaa
     9241 tgcactaaag catgatctga caacacccgt atcgtaacgt ctaaaggggc agttttagtg
     9301 atcttattac tgtttttaaa gtttcttcta aaaaatagcc atttcatttg tctgatgcga
     9361 ttagagtgat tataagtgaa aaaagaacag ccatctcatt ttgaatcaaa tgagatggct
     9421 gttctttttt ataagttatt gatcatttag atactactat ttacttgaaa tagcttcagc
     9481 ggcatcatcc attgaaagca cttcgtggaa gacacgttga gtcaattcag ttttttgttc
     9541 tggtgtaagg tatttggtgt tgacacagta accagagata cgcacgataa cgtcttcgcc
     9601 gttcatgatt ttgtcataaa catcgttaag gtccataacg ttcaagttaa cgtgctgacc
     9661 accattttca aagtaaccgt caagcactgt taccaagttg tcaacttgtt cgtcaaatgt
     9721 tttaccaaga gcacgaggtg atacttgagt agtcaatgag ataccatcgt tagcatgtga
     9781 gaattccagt ttagcaagtg agttaaggtt ttgtaaccag ccaccttttg ctttgttaga
     9841 tgggttagca cctggtgaga agaattccac ttggctagtg ttaactgtac catcttcgtt
     9901 caagaagacg ccgcggtgaa ctggagagtt accagtttgt ttagagtaag caacgtttga
     9961 agtgatggta agaagtgata ctgaagcctc agcattcttg taaagtttgt ggcttgcaag
    10021 acgtgtgtgg taagcttcca tcaaccattt cgcgatgtca tcaacacggt cgtcatcttc
    10081 accgtaacga gggaagtcac ctgttacttc gtagtcataa atgtagccat tttcatcgcg
    10141 gatagtttta acagttgcgt atttaatagc tgacaaggta tcaactgtgt tagcgaaacc
    10201 acagataccg aatcccatgt tagcacgttg gtgtgttggc aagaaggcca tttgaactgc
    10261 ttcgtagttg tatttatcag tcatgtagtg gatgatgtta agcgcatcta cataagtatc
    10321 tgtcaaccag tcaagagatt tctcaaagtt agccataact tcgtcgtact caaggacttc
    10381 tgatgtgatt ggttcaacaa cgttaaacac tttgtagtct ctgtgaacat catcgtaacc
    10441 accgttaaga ccagtaagaa gggctttgag aacgtttaca cgagcaccga agtattggat
    10501 gttatgacgt tgttcttcat tttctgggtc aagtggtgat acacaacatg aaatacatga
    10561 catttcacca taaccttctt tagccattgt tgtcacacct tcatattgga tagaagagtg
    10621 tttgtggctc attttcatac agtagcgacg gaatgtttct ggcagttggt cagtccaaag
    10681 aactgttaag tttggttctg gagagttacc gatattatca agagtgttta agaaacgata
    10741 gtccatctta gtcacacggt gacgaccgtc gttacccata ccagccatag aagttgtgat
    10801 gaatgttggg tcacctgagt aaagggcatc gtaagcttta gtacgaccaa attttacggt
    10861 acggagttta aggacaaaat catcaacaaa ttcttggatt tctgattctg taaaggtacc
    10921 gcgagcaagg tcacgttctg caaagatatc aagaacgatt ggcacacgtc caagagaagt
    10981 agcagcaccg ttgatcacac gacatacagc catgaaagcg atgtttaccc attggatagc
    11041 ttctttaacg ttttgtgctg ggcgacgaac atcaactccg taaaggtcac caagtttaac
    11101 cacttcacca agagcttggt attgaaggtt aacttcttcg cgaagacgaa tggattcttc
    11161 atcgatttca gtgatagcat tccagtcatt gactttttct tgcatcaagt agtcagcacc
    11221 gtaaagagca agacgagcgt aaacaccgat gatacgtcca cgtgagtaag catctggaag
    11281 accagttaca gtgtgggcat gacgagcacg acgaatattt gaagtataag cgcggaagat
    11341 accgtcgtta acagttgtca cgtacttagt aaagatttcg tgtactgctg ggtctggttc
    11401 atagccgttt tctttaaggg tcgtttccgc catacggata ccaccttttg gcatgaagtt
    11461 caatttgaac aattcatcgt tttgaatacc ataaattaat tcattttctt tatcgatgaa
    11521 accagcagga atgtcagcaa ttgaagttgg acgagtatcg tatgggaaac gtgtagcttc
    11581 atagtgtgct tttgtttctt cgataacttt tttgatgtga agtgaacgtt cagtagcacc
    11641 agccaagaaa gattcatcgc catcgtatgg ggtgtagtta gcttgtacaa aacgtgagac
    11701 acttgctttt tctttccagt cagttccttt aaagctttcc caagcttttt cgaaaacatc
    11761 tgtattggtt ttaacagttg ccataaattt ctccttaaat tctagtaaca aagcgatctg
    11821 ttacatttac aattctaagt ttaacacata ggaaacgttt tcccaagcaa aattcttgac
    11881 aaaattaaga ttcttttata atacagattg ttgaaacagt cacaaactgt attagaatgt
    11941 attttttttg gctttaggta gcctaaaagg tataataggg aaaggaggga cttatgctta
    12001 tttttccact gattaatgac acgtcacgaa aaatcatcca tattgatatg gatgcctttt
    12061 ttgctgcagt tgaggaaagg gataaccctg ctttaaaagg aaagcctgtt gtggttggga
    12121 aagatccaag agaaacaggt ggtcgcggag ttgtttccac ttgtaattac gaagcgagaa
    12181 aatatggcat tcattcggcc atgagctcta aggaagctta tgagcgttgt cccaaagcca
    12241 tttttatttc aggaaattat gaaaagtatc gaacagttgg ggaccagatc cgccgtattt
    12301 ttaagcgtta tactgatttg gttgaaccta tgtctattga cgaggcttac cttgatgtga
    12361 ctgataataa gttggggatt aagtcagccg tcaaaatagc caagctgatt cagcatgata
    12421 tctggaaaga agtaggattg acctgttcag caggtgtgtc ttataacaaa tttttggcta
    12481 aattggctag tgattttgaa aaaccccatg gcctcaccct agtcttgaaa gaagatgccc
    12541 tgtgcttttt agccaaactc cccattgaaa agtttcatgg tgttggtaaa aaatcagttg
    12601 aaaaactgca tgacatgggg atttatacag gacaggattt gttggcagtt cctgaaatga
    12661 ccttgattga tcattttggt cggtttggtt ttgaccttta ccgtaaagcg agaggcatca
    12721 gcaattctcc tgtcaagtct gatcggatac gcaagtcaat tggcagtgag agaacctacg
    12781 ctaaactgct ttatcaagaa acagacatca aggcagagat cagtaaaaat gctaagcgcg
    12841 tggccgctct cttacaggac cataaaaagt taggcaagac cattgtgctc aaagtgcgtt
    12901 atgctgattt taccaccttg acaaaacgtg tcaccttgcc agaattaacc agaaatgccg
    12961 cacaaattga gcaagtagct ggggatattt ttgacagctt aagcgaaaat cctgctggta
    13021 tccgcctgtt aggggtaacc atgaccaatt tggaggataa ggtagctgat atttccttgg
    13081 acctatagga gaaagagggc tctatccgtc cgcctcattt tggagaaaaa agaagctgat
    13141 tagggatttc cctaatcagc ttctttggtc cattatttta ttacagcata gcttggtctg
    13201 tatcatcacc tttttcgtta agggttttag ccttgtaaaa agcaaagcca taaagagcag
    13261 cttgtataag gataaggata gttccaacca ttgacaaggt tgttgtgaac tggttaacca
    13321 agccgataac agaaaggata agtcctaaga tgtaaggaat ataactaacc gttaatcctt
    13381 gttttagttt ggataagttt tgagcacaaa agacaatgat agcaataata gcaaggacat
    13441 ttaagacaga taagaaaatc ataaatggtg tcatgctgct ttgtagctgt gccaattgct
    13501 cagttgttaa ttgggcccga aatgcttcat tcttgagttg gctttgtaag tagaaaatcc
    13561 ctgcaaatcc cattaatgac agtacaactc caagcccgtt tagaataata atggcaatag
    13621 tagacgtttt gagtgcctga cgcacttttt cataagaaat catataatcc tcctagagtc
    13681 cttaatccga aattgtataa tgtgtttaat acaaaaatat tataccatag attaatcaaa
    13741 aatgtccgtt tttctaggat tttttctgga aaaagagggc aatatctgac tctgtaagcc
    13801 ctatcatcga atcgatagtc gaataattct caggtgttaa cctaaaatca tcattatctg
    13861 actgagagtt ctcagtgggg ttagcgtttg ttgattgaga ggaagatttt tgagagtcgt
    13921 cctcaagggt tgccgtatta gaaaaaaggg aggtttgctc ttttgatttt agctcttggt
    13981 taggttgaga ggaggctaag tcggattgtt cttggaagcg ttcgatcaag taggtttggc
    14041 gtttatcgcc ttcgtgttta atagcatact caaaggcagt atattctccc aacagaatta
    14101 acttactttt agaccgagta atggccgtgt aaatcacatt tcgttgcaag agtcggccgc
    14161 tttggcgtgt gataggtaaa atcaccactt gaaactcact cccttgcgat ttatgaatgc
    14221 tcatggcata ggccagggtt aattttagcc attcatttct agggtacgtg acttcgctac
    14281 cgtcaaaatc taagattaat tcgtcttgtt tagattcggt gtatttagca ggaatcaaat
    14341 ctgtaatata accaatatct ccgttaaaga cattcaactg agcatcgttg actaagtgca
    14401 ggactttatc acctttacga aaatgcgtat cattgaacag aaattctgtt tgtccttgca
    14461 agggattgag aagttcttgc atgagttgat taagatgatt gatgccagct tgtcctttgt
    14521 acataggcgc taaaatttga atttcctcag cagggatgcc acttttaata gcagatagga
    14581 caattttttg aatcatgtca gggataaagg ctgcttgggc ttcaaaataa gaacggtcag
    14641 cttttttatc acgaaagtca gcagctaaga ttcctcgacg catctgatct gccaaattga
    14701 caatagtaga ttcttgggat tgacgaaata tcttttgcaa ggcgatttgg ggcaggctat
    14761 tgacttttaa aagatctgac aggacttgac cgggtccaac cgaaggaagc tggtcactat
    14821 ctccgacaat aatcacttga gttgttgagt taatagctcc taacaactgg ttggcaagcc
    14881 aggtatccac cattgaaaat tcatcgacaa tcagcaagtc gcagtctaga taatcctcca
    14941 tggcttggta atcgttgtcg ccattgaggc ctaagtgcct gtggatggtt gcgctaggaa
    15001 gtccagtcaa ctcattcatg cgacgtgcag ctcgacctgt tggagctgct aacaagatag
    15061 gaaggtcttt tttatccaaa tcaatctgat gcaggttcgc gtaagcctgc aaaatgcctc
    15121 gaataacagt tgtttttcct gttccgggac cacctgttag gagaaagacc ttgctggtta
    15181 aggccttagt aatggcttct tgctgcactt ggtcataggt gatggcaaag tctttttgaa
    15241 cggcttggat ggttgtttga atggtatcat gactaaatga ctgacttaaa ggggtatcta
    15301 agagacgaga gatgttgtta gcaatccctt cttcagcaaa ataaaggcta gcatcaaaca
    15361 gtttggtgtc actgtttttg attttgccct ctatgattaa ctcgcttagt tgctcagcca
    15421 cagcagcagg atcacattct acttgacgtg catcttcaag gagtgtgatg gcaaagtcta
    15481 ataattgtcg tgcttgaaca taagtatctc ctcgattgat ggattcttcc aagagacaat
    15541 gaaggagggc tgcacgaaaa cgtttcggag agtcgctttc aatccctaag ttttcagcga
    15601 gagcatctgt cattttaaac ccaaaacctt gtagatcttc gactaactga tagggatttt
    15661 ctttaacaag gtcaagagcc tcttctttat acttttcaaa cgcttgaaag gcaaaacggt
    15721 tgctaagtcc aagttcgacg agaccagcaa tcagttgctc tgtgccgtaa ttcaatttta
    15781 gtttagcgac aaaggcttga cgattagcct tggacagacc agagatagtt tctaatttac
    15841 ttgggtcttc taaaatatgg tcaatggtat tatgaccata tagcgcaatg attttctccg
    15901 ctgttttttt accaatcccc ttaaaatggt cactagagaa ataattaacc aaaccagatg
    15961 aactaggttt gattttttgg taacggctta gcttgagttg ttgaccgtat ttagggtgct
    16021 gggtcaattc tccccaaaag gtgtagtcat ctccttcaat aatgtcagcc atcgttcctg
    16081 tgataatgat ttcaaagtcg tcaatgtcac tgtctgtatc ctcaatggca aggaggagaa
    16141 ttttaaagaa attagcctgg ttttcaaaaa tgatacgatc aacagtacct gtaaaaacgt
    16201 attccatagt aactctttct atttatacaa gataactgaa aacctgccca aaggcaggtt
    16261 ttttctaatt aaagacggtc attttattga gtggccaaaa acgagctttc acttcaccga
    16321 taaggttttc ttttttgaaa ctaccaactt cgcgactatc ccgggaaaca atacgatcat
    16381 caccaagaag aaggtattct ccttttggaa cactcatctc aaagcgtgtt tgtccctcag
    16441 aattagttgt aaaagcatca gatgtttctg ctaactgttg gaatagggta ttataggcgt
    16501 aagttttttg gagtttatcg tttttaaatt gttttagata ctcagccaaa tacggctcaa
    16561 ctgttttttt accattaata taaagtgtgt catcattata agaaatggta tcgcctggca
    16621 atccaataac tctttttacg atttctttct tttgtccgtt ttcttcttcc tgagcaacta
    16681 caatatcaaa gcgatcaatc ctagcttgat taaaaacgat aaggcgttcg ccatgagcta
    16741 gagttgggtc catagaatgg ccgtctactt taacagcctg ccacaaaaaa agacgagata
    16801 gaccaaagag aattaaaaag agagtgaatg ggccccattc tttaataaac tgtttcatat
    16861 gattcctttt atttagtgag aagcgcttgc gctttgtgag tgttggcgaa gtgaagttta
    16921 gcactatatt ctaagctaga cataccataa gcggctaaga gttggctagc aactttatct
    16981 gaagctgaac cagcaccgct tgggagttgg taacctaaat cttgacctaa ttgatcgaga
    17041 ttgtctaaaa atagatttct agcaatgata gagctaacag ccacagccaa atagtgactt
    17101 tctgcctttt cttgaaaggt tagaggattt gggaaatggt ttttttcctt ttttagatgc
    17161 ttttcataat ttgattggct agtgaaagca tcaataacaa tttgtttagg ttgaatgcct
    17221 ttttgtaaca acaaaaaaat agcttggtta tgaagagcaa ctttgataga aatggcatta
    17281 tatggtttac ttttaccaac caattcgtta tattttttag gtgataggag aagtgattga
    17341 tgaggtattt gtttctctaa cagaggcgca atctgttgaa tggttttatc cgataatttt
    17401 ttagaatcat caaccccaag ctttttcagg aagggatgat ctttaggatc gacaaaactt
    17461 gccacaacag caatcccacc aaaataagag ccattaccca cctcatcact gccgattata
    17521 ggtatatctt ggctattgtt gcttgcttca aaaactgtct tagctactgg aagattaagc
    17581 tcttgagcta aagcatttgc accgtttccc tggagaacta attttccgga tttgtacagc
    17641 aaaacagtaa ccccattttt tttagcagca aaggctacat aggtattttg gctactgatg
    17701 gtgtaggggg ctagctgttt ttttaaatgt ttactcagta tagcatctat tttcaaaact
    17761 aaggtattca taataattat tttaacataa aatagttgac tatggttaat tcataagttt
    17821 atttaacaaa gaaaacagag actcgaatgt ttagacgggt tagggcaatg gtgttgacta
    17881 ttttacgaaa gtttcatttt acggtataat gctaatgagg tgaccctatg aaaagtatta
    17941 atcgctacaa attcaccttt ggtgaaaaaa cattaacatt gacaactgac aaagacaatc
    18001 tctttatgga agaagttgag cgggtagcta aagaaaagta tcaggctctt aaaaatcatt
    18061 taccagaagc tgatgacgag actatcgcta ttttaatggc tatcaatacc ttgtcaacac
    18121 aattaagcag agaaattgcc attgaaaaaa tggaagctga aattttagat ttacgtcaaa
    18181 aaaccttggt tggtcttcaa gaaaaagcta atcaaataga tgtgaatgag gtttaatcat
    18241 gttatcttta ttgatagtgc ttgttttaac ttggaatttt tatattggtt atagtagagg
    18301 tattattctt caatcgtttt atgtcttagg agccctactt tctcttttgg tagctaatcg
    18361 attttatatt ggattagccc ataagctaac gctttggatt ccttattcta acccggtaga
    18421 aggaacatca gtctttttct ttaaatcagt tgatatcttt gttttagata aggtttatta
    18481 tgcaggcctt gcctttttta tcattttctt attggggtat gcattgagtc gctttcttgg
    18541 gatttttgtt catttccttt tgctagatta ttttgataac cagtggacaa aatgtttgag
    18601 tggcggacta gcgcttctag tatcgcttct ttttttgaac atgttactgt ctatttttgc
    18661 aacggtccct atgccttttt tacagcacta tttgcactct agttttcttg ctcgtttggt
    18721 gatcgaacac cttccaccat tgacaataat tattcaaaaa ttgtggattc aagccattat
    18781 ttgatatcgt tttgtgacta atataagaac ctggagtgag tttcaccttt aggttcttta
    18841 tttacgagaa actgtttaga aataaagaga acgcatggga acttagtgac tttcttgcca
    18901 cattttccat cattttaggt ataatgaaga gtatgaataa caagatttta gagcagttag
    18961 aatttaacaa agttaaggaa ttgctattac cttatctcaa gacagaacaa tcccaagaag
    19021 aattattaga gctggagccg atgacggagg ctcctaaaat agaaaaaagt tttaatgaaa
    19081 tttctgacat ggaacagatt tttgttgaac atcactcatt tggcatagtc agcctaagtt
    19141 caatctctga gagtttaaaa cgcttagaac tttcagctga tcttaatatt caagaacttt
    19201 tggctatcaa aaaagtttta cagagttctt cggatatgat tcacttttat tctgatttgg
    19261 ataatgtttc tttccaatct ttggatcgtt tgtttgaaaa tttggaacaa ttccctaatc
    19321 tgcaagggtc ttttcaagct atcaatgatg gtggtttttt agaacatttt gcgagtccag
    19381 aattagagcg tatccgtcgt caattaacaa atagtgaacg acgggttcgt cagattttac
    19441 aggatatgct taaggaaaaa gcagagcttt tatcagaaaa tctaatcgct agtcgtagtg
    19501 gacgaagtgt cctaccagta aaaaatactt atcggaatcg tatttctggt gtggttcatg
    19561 acatctcttc ttcgggaagt actgtttata ttgagcctcg tgctgtagtt acactaaacg
    19621 aagagataac gcagcttaga gctgacgaac gtcatgaaga aggtcgtatt ttacacgcat
    19681 tttcagactt gttaagacct catgtcgcca ctattagaaa taatgcatgg attcttgggc
    19741 atcttgattt tgtaagggct aaatatcttt ttatgtctga taataaggcg acgataccta
    19801 agatctctga tgacagcacg ttagcattaa tcaatgttcg tcatcctctt ttaagtaatc
    19861 ctgtggctaa tgacttacat tttgatcacg atttaactgc aattgtcatt actggtccca
    19921 atactggtgg taagacgatt atgctaaaaa cactcggttt agcacaatta atgggacagt
    19981 ctggtttgcc agtattagcg gataaaggta gtaaaattgc agtatttaac aatatctttg
    20041 cagatattgg cgatgagcaa tctattgaac aaagtctatc aactttttct agtcatatga
    20101 cgcacatagt cagtatttta aacgaggctg accacaatag tttagttctc tttgatgaac
    20161 taggagcagg aacggatcct caagaaggtg ctagtttggc catggctatt ttagaacacc
    20221 ttaggttaag tcatatcaaa acgatggcga ccacgcacta tccagaatta aaagcttatg
    20281 ggattgagac aaattttgta gagaatgcga gcatggaatt tgatgcagaa acgcttagcc
    20341 ccacatatcg ctttatgcaa ggagttcctg gacgctcaaa tgcatttgaa attgcttctc
    20401 gccttggttt agctccattt attgttaaac aagctaagca gatgacagat tctgactcag
    20461 atgttaaccg tattattgaa cagttagagg cacagacact tgagacacgt agaagactgg
    20521 atcatattaa agaagttgaa caagaaaacc tcaaattcaa tcgtgcggtt aagaaactct
    20581 ataatgaatt ttcacatgaa cgcgataaag agttagaaaa aatctatcaa gaagctcaag
    20641 aaattgtaga tatggctttg aatgagagtg atactatctt aaaaaaactc aatgataaga
    20701 gtcaattaaa acctcacgaa attatagatg ctaaggcaca aataaaaaaa ttagcacctc
    20761 aagttgattt atcaaaaaat aaagtcttaa ataaggctaa aaaaatcaaa gcagctcgtg
    20821 cccctagaat tggtgatgat attatagtga ctagttatgg acagcgaggt accttaacta
    20881 gtcaattaaa agatggacgt tgggaagcac aagtgggaat tatcaaaatg acattaacac
    20941 aagatgaatt tacccttgtt agagtccaag aagaacagaa agtcaaaagt aaacagatta
    21001 atgtggttaa aaaggctgat agttctggac caagagctcg acttgatctt agaggtaaaa
    21061 gatacgaaga agcaatgcaa gagttagatc attttattga tcaagcattg cttaacaata
    21121 tgggacaagt tgatatcatt catggtattg gtacaggcgt tattcgtgag ggagtgacaa
    21181 aatatcttcg tcgtcataag catgttaagc attttgctta tgccccacaa aatgcagggg
    21241 gatctggtgc cacaattgta acgttagggt aaatgatgga atcatatgaa cacttttatg
    21301 ccaaactatc tcaacctttt agaaaatccc cacagttaat aattctttta aatgttttac
    21361 taaaaataat cacaggagtg atgtacatac tttatccatc ttttctgata tttacgcttt
    21421 ggcaagggat gacatttcaa ttatggttaa ggctattaat tattcctgct gttggcttta
    21481 tagctttgtc ttacattagg aaacgctttg attttccacg accgtatgaa aaatggaata
    21541 ttaaaccttt aattgataag gatacaaagg gaaggtcaat gcctagcaga catgtttttt
    21601 cagctacgat gattagtatg tgtttgttac gatattatgt ttactttgga atagtttgtt
    21661 taatattatc ggttttattg gcaatttgcc gtgtgatagc aggtattcac tatcctaagg
    21721 atgttattgt aggttacctt attggcttga tgttagggct ttgtttattg atttagcgag
    21781 ttcgtagcaa atagtttcaa aaacaactaa attcttgtac aataggctta tcattaagat
    21841 gaaatggaga aaaaagatgg cattagaagt aacagatgct acatttgtag aagaaactaa
    21901 agaaggttta gtactcattg atttttgggc aacttggtgt ggtccatgtc gtatgcaaac
    21961 accaatctta gaacagttat ctcaagaaat cgatgaagat gaactcaaaa ttttgaaaat
    22021 ggatgttgat gaaaatccgg aaactgctcg tcagtttggt attatgtcaa ttccaacatt
    22081 gatgtttaaa aaagacggag aagtggttaa acaagttgca ggtgttcata ctaaagacca
    22141 actgaaagct attattgctg aattgagtta actaaaataa agcccagcgt cacgctgagc
    22201 tttattttat gacctactaa cttcaatgga ataaccatgt gaacataaaa cgacttgcaa
    22261 aagcaatagc aacagtaatg aggaaaaata cagtgacaat aagggggttc tgataaggaa
    22321 gttttgctct ttcttctcgt ttttcgattt cgtctaataa ttttccactt gaaacagcta
    22381 ggatttcctt ataaggttgc acatcatatt ttttcttaaa acgttccaca cgattagcat
    22441 aagtcatagt aattatcgat aataaaacaa aaatgccaag tcctagccac tctaagaaat
    22501 agactaatgg ataacttgaa atcattaata gtatcatcaa tgttaccatt actttggtat
    22561 ctcgtttaaa attcttttta tcaacctgag tgatggtata tttcattttc tcaatgtctc
    22621 ttttaatgag ttggtctaag ctcacttgaa aaatttgact taggagtaag aggctatgga
    22681 tgtctgggta agttttgtca ttttcccagt tagatatgct ctgacggctt acatagactt
    22741 tctcggctaa ttcttcttgc gagagcgctt gttgtttacg gtagcgaata atttgctgac
    22801 caatttccat aaatatctcc tttcataata ttagtataag gatgataagc taacgggtct
    22861 atcaaatgta tttgacatta gggatagacc actatttata gggtattaaa agataatctg
    22921 aggaaaactt tacagcgacg taaaggaatt gtaaacaaaa agtgaaccgt catagttcac
    22981 ttgtgattaa gtatttttct gttttaagta ggtctctagc attttctttt ggggcgttgc
    23041 aaagggataa agagaaaagt cctcaattgc tacccacttt aggtgaggag cattaggtaa
    23101 atctgttgct ttgaccaccc cctctatgag ttctattgtc cacttctggt gactaaaagt
    23161 gtgcttaata tttggaaagt gattatcagt ccattggggc tttaattgat attctctttc
    23221 gaaagtttca ttttgggttt gccagattat ggggttggat tgattgtcat caaataaatc
    23281 taattgttga gaaagaggtg acgtttcgat aataggaaat gaccaaaaac ctcctagaag
    23341 tctcccttta gtatttttct ccagtagata ttggccattt tggttacgaa tgacaaaagc
    23401 ctgaattcgc attggtttag gctttttctt tggatttttt atgggatatt taccataagt
    23461 accattcaaa taagcagcgt taaagaaacg aatagggctt tcatcaggcc taggtgtctt
    23521 agcggactcg atgtctgtac ctagatccat aagtgcctgg ttaaaatcac cgggtctgtc
    23581 agggtcaatt agtatttcca taatagcttg aaaaatttta cgatttttag ggtcaccgat
    23641 atcataatta acttcaaaca aacgagccat gacacgcatc acattgccat caacagcagg
    23701 ctctggtaag ttgaatgaga tgctggcgat agcacctgca gtataaggac caatcccttt
    23761 taatgaagca atatcatcat aagtgtgagg aaatatgcca ccaaaatcaa ccatcacttg
    23821 ctgtgcagcc ttttgcatat tacgcacccg agaataatag cctagtccct cccatgcctt
    23881 tagtaattgt tcttcaggag cgtctgccaa atcttttatt tgaggaaacc actctagaaa
    23941 acgtttataa taagggatta ccgtattaac ttgtgtttgt tgcaacatga tttcagaaac
    24001 ccaaatataa tatggattag tagttcttcg ccaagggaga tcacgttttt cttggtcata
    24061 ccaccctaaa agtgtgcgac gaaaagaagc gattgtctca ttatcccaca tattaatacc
    24121 ataatctttt aattcaatca tgttgttatt gtaacacagg agccttaaaa agagtaaaaa
    24181 aagattttag gtatttgcga aaaagaaaag gaaatattat aataatatta ggtttacctc
    24241 atctaaatgt tgatttagaa taactgaaat taacgataga agcagacagg ttttaatttg
    24301 gaggggacat gttccagaaa ttaaagaatc ttattgatcg gtattcgtgg ttcgttttta
    24361 taatgatgat tcttgtgtta tttcttaact atatttatcc atttgatcct tctttgagcg
    24421 gactacagtt ggaatggctg atcatgacgc aagtttttcc tggattaggt atcgtatgtg
    24481 ccatggttgc tattattttt aaaaagttta acctttttgt tttggcccta attttatatt
    24541 ttgcactttg gttaaaactt ttccttgctt tttctttttt cacctatttt ttaggagtct
    24601 gactaagtgc caatttttaa aactcgtgaa agaaaatgtt tgacaaaact tagtcaagta
    24661 gaaaaagttc cttgctttta attgactatt agtgtacaat aagattctgt gagtatacct
    24721 cacttactca ctccaaaagt agtgagtcat tagaccaaaa ggaggaaaat atcaatggct
    24781 aaatacgaaa ttctttatat cattcgtcca aacattgaag aagaagctaa aaacgctttg
    24841 gtagcacgct ttgactctat cttgactgac aacggtgcaa ctgttgttga atcaaaagat
    24901 tgggaaaaac gtcgtcttgc atacgaaatc aacgatttcc gtgaaggact ttaccacatc
    24961 gttaaccttg aagcgactga cgcagcagct cttaacgagt ttgaccgtct ttcaaaaatc
    25021 aatggtgaca ttcttcgtca catgatcgtt aaacttgacg cttaattcca actaagaggg
    25081 tagtttatga ttaataatgt agtactagtt ggtcgtatga ccaaggatgc agaacttcgt
    25141 tacacaccaa gtcaagtagc tgtggctacc ttcacacttg ctgttaaccg tacctttaaa
    25201 agccaaaatg gtgaacgcga ggcagatttc attaactgtg tgatctggcg tcaaccggct
    25261 gaaaatttag cgaactgggc taaaaaaggt gccttgatcg gagttacggg tcgtattcag
    25321 acacgtaact acgaaaacca acaaggacaa cgtgtctatg taacagaagt tgttgcagat
    25381 aatttccaaa tgttggaaag tcgtgctaca cgtgaaggtg gctcaactgg ctcatttaat
    25441 ggtggtttta acaataacac ttcatcatca aacagttact cagcgcctgc acaacaaacg
    25501 cctaactttg gaagagatga tagcccattt gggaactcaa acccgatgga tatctcagat
    25561 gacgatcttc cattctagtc agaatggatt gaacgaatac caaattattt gagttcgggc
    25621 taaaaattta gaacttagaa tttagatcaa ctccctagga aatcaagatt tcttgccagt
    25681 tttctaaaat tcagtcgttt tttaagagcc cttaacatca taatttataa aggagaaaat
    25741 acatggctca acagcgtcgt ggcggattca aacgccgtaa aaaagttgac ttcatcgcag
    25801 ctaacaaaat tgaatatgtt gattacaaag atactgagct tcttagccgt ttcgtttcag
    25861 aacgtggaaa aattcttcct cgtcgtgtaa caggaacttc agctaaaaac caacgtaaag
    25921 taacaacagc aatcaaacgt gctcgcgtta tggctcttat gccttacgta aacgaagact
    25981 aataaacaaa cagtccattt cggactgttt tttagttgac taggactagt ctaggcgtat
    26041 aggtgagttt tttctaaaag agagaatgat agtaaacgaa aatccttgcg tagacagcaa
    26101 ggatgaaatg attgttattt gttaacaggt gacatagtgt tttgaatgtt gtatttgcgt
    26161 tgtaggtaat agcgtaaagc aagggaaaca ccaccaataa tgagcaaggc aagaggaggc
    26221 aactgtgggt taagagaggt tggtaaaaag gcagttgcag agtacaaggc aatccaaatc
    26281 aacatggcta atgataaggc tgcgatgatt ttgaaccaac ttggacgaag gctcttatct
    26341 tttccgagat gacggtaaat aaagtaataa gtagcgtaca tggatgctcc tccaccaaaa
    26401 ccaagtgcca gtaaggaaat caatcctgta actgtagcgt ttgtgttgaa gaaggtcatg
    26461 atgccattta aaagggcaac aattccgatg aatagcaaag aggtatctag ccacatcaac
    26521 caaggattgg tgtttttgtc agtttctgct gctctttgac tagggtcttg gctaaaagaa
    26581 gctgcccaag cagtcggagt gcctaataga tttcgagcgg tgacaccttt tttctggttt
    26641 tctagaatag ctggaatagc ttcttcgaga atcagcttaa tgtcctcatc agacttgccg
    26701 tcttggatta atttgttcgt agcggtgtgg ataaattctt gatttttttt ggtaagttct
    26761 tgtaattcca tgggaatccc ttctttatat gggttagtta aagtaagtca atggtattag
    26821 ctagtttcaa atgagacttt ggagccagtc gtttgacaca gagttttgtc aggtgttaac
    26881 cgcattctgc agcttgctcc actctagaac catttttttc taataaagta aatcacaaca
    26941 aaggaactaa ggagcatagc aatcaaggta atataccaaa aagcatgttc taggccattt
    27001 agtggtaacc agttgttttg gaaattcata ccataggcgg agaaaatcac agttggtata
    27061 tctaaagcca tggtcatcaa ggccaatgtt ttcataatgg tgttctggtt attgttgata
    27121 atggaagcag tggtttcagt catggcattc aagacatttt cataaatgcc agccatctca
    27181 atggcctgtt gggtttcaat caaggtatct tctagcaaat cctcgtcctc aatgtatttc
    27241 ttgagagagc ttgttgagct ggataatttc ttgacgatgc gttcgttgaa tttcaaggaa
    27301 gcctttaagt agacaatgga tttttcaagt tccatcatgt cgataagttc ttcatttcga
    27361 gtagctgctt caagctgagc ttccaaacgt tcgctttggc gatcaatggt cctcaaggca
    27421 gtcagaaaga gctcggcgtt gcgatataga atctgaaaaa caaaacgtgt cttcatgaag
    27481 gtatagaaat ttttgacccg gcggttgtga aaatgatcga aaagggtcat gtcatgcaga
    27541 caagttgtga taacagcatt ttccgttaca ataatgccta aaggcatagt aatatagtaa
    27601 cttttattgt tacgttcttc gtatatcggg acgtctacga taattagggt ataatcatct
    27661 tctacggcaa tacgtgaggt ttcttccacg tccaaagggg cgcgcaagtc agaaatatca
    27721 atgttgaatt gatcggcgat cttcatagac tcttcttggg aagggttaac cagtttaatc
    27781 caagctcctg gctcgaatgt ttctatttct ttgaattcaa ttgccgatga aagaaacatt
    27841 tgtttcatga caagccctcc tttttgatga ttgactggta gagattgaca tcaaaccttg
    27901 ataataaagc cttctcaagg attttctgaa aaattctcaa tacttatcta ttataccaaa
    27961 attcgcaaag tttggggcaa taggttatca gaaatttttg ttataatata agaaagctcg
    28021 atgaggaggg tgcctttagg cacattcaag atgcaaaata aaataataat taatggggca
    28081 agagcccaca atttaaaaaa tattgacgtg gaaattccac gagacaaatt agttgtcgtg
    28141 acagggttat cagggtcagg aaaatctagt ctagcctttg atactattta tgcggaaggg
    28201 caacgtcgtt acgtagaaag tttatcagct tatgctagac agttcttggg taatatggaa
    28261 aaaccagatg tggattccat tgacggtctt agccctgcta tttccattga ccaaaaaacc
    28321 accagtaaga accctcgctc aacggttggt acggttacgg aaatcaatga ttatttacgt
    28381 ttactatatg cgcgtgtggg cacaccttac tgtatcaatg ggcatggtgc tatcacggcg
    28441 tcttctgcag aacaaattgt agagcaggtc ttagccctcc ctgaacggac acgcatgcag
    28501 attttagctc ctgttgttcg tcggaagaag gggcaacaca aaacagtttt tgagaaaatt
    28561 caaaaagacg gctatgtccg tgtgcgcgtt gacggggata tttttgacgt gacaggagta
    28621 ccagagttgt ccaaaagtaa gatgcacaac attgaggtgg ttattgaccg tctggttaat
    28681 aaggatggta ttcgtagccg tttatttgat tcggttgaag cagctttaag gctgggagat
    28741 ggctacctta tgattgacac tatggatggc aatgaactgc tcttttctga gcattattcc
    28801 tgtcctgtct gtgggtttac ggttcctgaa ttagaaccaa gactcttctc ttttaatgcg
    28861 ccgttcggct cttgtccgac ctgtgatggg ctaggcatta agttggaagt ggatttggac
    28921 ttggttgttc ctgacccaag taagtctttg aaagagggag ctttggcgcc ttggaatcct
    28981 atttcctcca attattaccc aaccatgctt gagcaagcta tggcaagttt tggggtggat
    29041 atggatacac cttttgaggc cttgactgag gaagaaaggg atttagttct ttatggttca
    29101 ggggatagag aatttcattt tcactatgtg aatgattttg gtggtgagcg taacattgac
    29161 attccttttg aaggtgttgt gactaatgtt aatcgccgtt atcacgaaac taatagtgat
    29221 tatacgcgca atgtgatgcg tggttatatg aacgaattga cctgtgcgac ttgtcatggt
    29281 taccgtttga atgaccaagc cttgtgtgtg catgtgggtg gcgaagaagg acctcatatt
    29341 ggtcaaattt cagaattatc gattgccgac cacttgcagt tgttggaaga actggaactc
    29401 acagaaaatg aaagtaccat tgctaaaccg attgtcaaag aaattcatga ccgcctgacc
    29461 ttcttaaata atgtgggact gaattatctg accttatcac gtgctgctgg gactttatca
    29521 ggtggggaaa gtcaacgaat caggttagcg acacagattg gttctaactt atcaggggtg
    29581 ctctatatct tggatgagcc ttctattgga ttgcaccaaa gagacaatga ccgtttgatc
    29641 gaaagcctga aaaaaatgcg tgatttgggt aataccttaa ttgtggtaga acacgatgaa
    29701 gacaccatga tgcaggcaga ctggctgatt gatgtggggc ctggggcagg tgagttcggt
    29761 ggagaaatta ttgcctctgg aacgccgaaa caagtggcta agaataaaaa atccatcacg
    29821 ggtcaatatt tgtctggaaa aaaattcatt ccagtgccat tagagcgtcg ttctggtaat
    29881 gggcgattca ttgaaataaa aggagcagcg cagaataatc ttcaaagcct cgacgtgaga
    29941 ttcccactgg gtaaatttat tgcggtcaca ggggtatcag gctcagggaa atcaaccttg
    30001 gttaatagta ttttgaaaaa ggctgtcgcc caaaaattaa accgtaatgc tgataagcct
    30061 ggtaaatatc actctatttc tggtattgaa catattgaac gcttgattga cattgaccaa
    30121 agtccgattg gaaggacgcc acggtcaaat ccagccacct atacgggtgt ctttgatgat
    30181 attcgagact tgtttgccca aaccaacgaa gctaagattc gtggctacaa aaaaggccgt
    30241 ttctctttta atgtcaaagg tgggcgttgt gaggcttgtt caggtgatgg gattatcaaa
    30301 atcgaaatgc actttttgcc agatgtttat gtgccttgtg aggtttgcca cggcagacgg
    30361 tataattctg agacgcttga ggtgcattat aaagaaaaaa atattgctga agtgcttgac
    30421 atgactgtcg atgatgcctt ggtatttttc tctgctatcc ctaaaattgc ccgaaaaatt
    30481 caaaccatta aggatgttgg tttgggttat gtgaccttgg gtcaaccagc tactactcta
    30541 tctggaggag aagcgcagcg gatgaaatta gctagtgaat tgcacaaacg ctcgacaggt
    30601 aagagcttgt atatcctaga tgagccaacg actggtttac acacagatga tattgcaagg
    30661 ctattaaaag ttttggaacg ctttgtagac gatggtaata cggttcttgt gattgagcat
    30721 aatttggacg ttatcaaatc tgctgaccat attattgact taggaccaga aggtggtgtc
    30781 ggaggtggtc aaattgttgc cacggggaca ccagaagagg tagctcaagt taaagaaagc
    30841 tatactggtc attatttgaa agtaaaacta caacaatagg attaggagtt cgtttgagct
    30901 cctttttaat cttttaaatg ctctcaaacc atggtaaact taaagtagtt ttttcatgct
    30961 acaatattct caaaagttac caaaggagtt cgcgatgtca ggatttttag agcaacgatt
    31021 aggtcactgc ctaaggcaga tggcagagaa ggggctagag gctcttctag tcacccattt
    31081 aaccaatagt tattacttga caggtttttc tggaactgca gcaactgttt tgataacggc
    31141 caaacgtcgt gttttgatca cagattcacg ttataccttg cttgctaaag ctagtgttga
    31201 gggatttgat attatcgaaa gccgcacgcc cctgaaggtt gtggcagaat tgttaggggc
    31261 tgatcaaata gattgccttg gttttgagga ccaggtatcg ttttcttttt atcaggccat
    31321 gcaagcagaa ctgtcaggaa taaccttgct tgctcagtca ggctttgtgg agcatttacg
    31381 tcttattaag gacgtctctg aaattgagac cattgctaaa gcgtgctcga tctcagacaa
    31441 agcatttgaa gatgctcttg attttattaa accagggaca accactgaac gtgacctggc
    31501 taatttttta gattttcgca tgcgtcagta tggtgccagc ggcacatcat ttgatatcat
    31561 tgcagcttca ggctatcgct ctgccatgcc tcatggacgc gccagtgaca aggttatcca
    31621 gaataaagag agcttgacca tggactttgg gtgttactac aatcactatg ttagtgatat
    31681 gacgaggacc attcacattg gccaagttac tgatgaagaa cgtgagattt atgctcttgt
    31741 tcttgctgct aataaggctc taattgctaa agctagcgct ggcatgactt atagtgactt
    31801 tgacggtatt ccgcgccaac ttatcactga ggcgggttat ggcagtcact tcacacatgg
    31861 cattggtcat ggtatcgggc ttgacatcca tgagaatcca tttttgggga aatctgagca
    31921 acttctccaa gctggaatgg tggtcacaga cgagccaggt atctatttgg ataacaaata
    31981 tggtgtccgt attgaagatg acttggttat cacaaaaact ggttgtcaag tcttgacctt
    32041 ggcacccaaa gaattaattg tattgtaaaa gaatggttca ggaaaggatt aggggagaag
    32101 caatgacaaa tcgtttatca tggcaagact attttatggc caatgcagag ctgatttcca
    32161 agcgctctac ctgtgatcgc gcctttgtgg gagctgtttt ggtaaaggat aaccgcatta
    32221 ttgccacagg ttacaatggc ggagtttctg ctacggataa ctgcaacgaa gcgggtcatt
    32281 atatggaaga cggtcattgt attcgcactg ttcatgctga aatgaatgct ttgattcaat
    32341 gtgcgaaaga aggaatttca acggacggaa ctgaaattta cgtgactcat tttccttgta
    32401 ttaactgcac taaggcgctt ttgcaggcgg ggattacaaa gattacctat aaagcacatt
    32461 accgtccgca cccctttgcc attgaactga tggagaaaaa aggggttgcc tatgtgcaac
    32521 atgatgtgcc tcaaattgtt ttaggaggcg ataaggaaca atagaaagtg tacctaaaag
    32581 gacggtcata aaagatttga gccaaggcca cttttgtggt aaaatgaagt gataaatatt
    32641 ttagtaagag gtaataataa tgattgaagc aagtaagctt aaagcaggta tgacatttga
    32701 agcagaagga aaattaattc gtgtccttga agctagccac cacaaaccag gtaaaggaaa
    32761 cactatcatg cgtatgaaac tacgtgatgt gcgtacaggt tctacttttg acacaactta
    32821 ccgcccagat gaaaaatttg agcaagccat cattgaaact gtcccagcac aatacctata
    32881 caaaatggat gacactgctt acttcatgaa cactgatact tatgatcagt acgaaattcc
    32941 agttgctaac gttgagcaag aattgcttta cattcttgaa aactcagacg tgaaaatcca
    33001 attttatgga agtgaagtga ttggggtaac ggttccaaca actgttgaat tgaccgttgc
    33061 ggaaacacaa ccatctatta aaggagcgac agtgacgggt tcagggaaac ctgcaactct
    33121 tgagacagga cttgttgtta acgttccaga ctttatcgaa gctggccaaa aactaatcat
    33181 taacactgca gaaggtactt acgtttctcg tgcttaatcc tttgtcaaaa cagattagtc
    33241 taaaaaagag aaaggaaatg ctatgacaac tgaatatatc ggtgaaatcg tcatttcacc
    33301 acgagtgctt gaggttatta cggggattgc tacgacacaa gttgagggcg tccactcact
    33361 ccataacaag aaaatggcag atagctttaa caaagcaagt cttggtaaag gggtttatct
    33421 ccagacagaa gaagatggaa gtgttacagc agatatttat gtttacttgc aatatggagt
    33481 caaggtcccg actgtatcta tgaatattca aaaaacagtc aaatctgctg tgtacgatat
    33541 ggcagaagtg cctatttctg cagttaatat ccatgttgaa ggtattgttg cggaaaagac
    33601 accaaaacca gatttgaagt cactttttga tgaggatttc ttggatgact aacagctttc
    33661 aaaattcaag aagagatttg cgtgaacgtg cctttcaggc tttatttaac atagagatgg
    33721 gagctgagtt attggcagct tctcagtttg cctatggtta tgataaagta acaggggagg
    33781 acgctcaagt attggagctg ccgatttttt tattaagtct tgtaacgggt gtcaataatc
    33841 ataaagaaga gttggataac ttgatatcaa ctcatttgaa aaaaggttgg tcgttagaac
    33901 gcttgacact aacagacaaa acgttattac gactaggtct ttttgaaatc aagtattttg
    33961 acgaaacacc agaccgtgtt gcactaaacg aaatcattga agtagctaaa aaatattctg
    34021 atgaaacctc tgctaaattt attaatggct tacttagcca atatgtctcg gaagctcctt
    34081 cagcaaataa atcataataa gtcggagcaa tcttaggatt gtatccgatt ttttcaattt
    34141 atgtacgaat attaaaagac aaacaagcat actctcgcat ctagaaagag tatgcttgtt
    34201 tgtcttttag agtaattagt gattggttat gttttgttta aatacttgct cctggaagta
    34261 gcgtaatcgg taaaatatag tctttactgg ttttttcttt tttaattttt ttgatgagta
    34321 tttcgacact caatttagca atctcatcaa taggttgacg aatggtcgct aattgaggaa
    34381 catattgctg gataaatgtc gttccatcat aacctatgac tttcatatcc tctggaatag
    34441 taatgtgaag ttgtttggct actttcataa tgaggatagc agttaaatca tcagaaacga
    34501 aaaggccatc aggttttcgg gttgccaaaa tggatttaat ttccatttcg cgtctaacag
    34561 gagataggtt atttggtaat ttgaggattt ctgcacttcg tttgagctgg tagttaaacc
    34621 ctagctggcg caaccctgtg ggcgaatcag aattgtcatt acctgtaatc atgacaatgt
    34681 tttgacaacc gtgtttttgc aaagtttgtg cggctaattt accaccttca aaattatctg
    34741 aagaaataac cgggatattt ggcgccagat tacggtcaaa agctacaata ggagcttcga
    34801 cacgctcata atcctcaatt cccaaattat gactggaaga aataatgccg tccacttgat
    34861 tggccgcaag catttctaag tattcgcgct ctttgacggg attatgttca ctattgcaaa
    34921 taatagtttt gtagccctgt ttaaacaatt caatttccaa atgttcaatt aattcagcgt
    34981 aaaagatatt gctaatgttg gggaagataa gcccaatgag ttgggttgat ttaccttgca
    35041 aacttctagc gagattgtta ggcttgtagc ccaattcacg catggcctta ttcaccttat
    35101 tcactgtttt ttgggaaagg tagccttttt tgttgattac tcgcgaaaca gttgttggac
    35161 tgacacccgc caaggctgcc acgtctgtta attttgcgac cacttgccta atacctcatt
    35221 tcataataat gtccaaaagc ttgtccttct actacttgga tacctgtttg tccttgggtt
    35281 gggaaaacac gactcgttag gactttttgc ccttggttaa taaagatttc aagaatggaa
    35341 ttgtccacat agacattaag tgtaacatgg ccttgtggga tttggcaaga gcgcaccgta
    35401 ccatagtctt gggcatattg aacccctgcc cgacttcggt caatgcttaa ctgtcctttt
    35461 gttgtatcca ctttcaatct taggccattc cctttttggt cagcaaatag gagcaggtca
    35521 agcttttttt ggcctggaat cgtcaattcc aattcataac agttagaact gtctatttta
    35581 ttatgaaata attctgctgg ccctcgaaga ttttgtagag ctgaaacagg tgtttgatag
    35641 agaattccgt ctttaatcct taattctttc actaaactaa gagctccttg atagttataa
    35701 cgatctgtcg gataatcaat atctggtaac ccaatccaag acacggctag aaccctgcca
    35761 tctggactac taaaggcttg agtggcataa gcttcaaaac caaagtcaag attttgaagg
    35821 gcccccccac ccagcaattg gccagtttct ggattgaagc tttcaaaaat tttataggta
    35881 ttggggtaga tattttgata atctaaatca gctttagcca gtccttgtgg gctgaaaatc
    35941 aaaacaggtt tgtcatcgac aaacacaagg ttggggcatt caatcatgta ttccgtacca
    36001 gaatcgtcaa agtccaaatc ggctataaat cgccagttgt ccacatgatt gtccacagct
    36061 ttgtaaagtt tgatttttcc tttgccatct aaaccttggg cgccaataat agcatagaat
    36121 tgaccttgat aagaaaaaag ctgaggatcc cgaaaatgtt ctgtaacatc atcgggctgt
    36181 tcaatcaaaa cctgcgggat tttggaaata tttccttgtt tatccatcca tgcgccaacc
    36241 tgtaatggtg ttctgaccca gttggcattg cgaacattac cagtgtaaaa gaggaagagc
    36301 ttgtcatcaa tagcataggc agaacctgag taagccccat gactatcgtg agcgtggtct
    36361 ggcagaaggc ggctccttgt ttccgtaaaa tggacgaggt ctgtggatgt catgtgtacc
    36421 cattgtttca gaccatgcgc agcgccataa ggccagtttt gataaaaaag atggtagcga
    36481 ccgttgaaat aagaaaaccc attggggtca ttgagtaatc ctgtcttagg ttcaacatga
    36541 aattggctgt gccatgggga ttgggccatt ttttcagtaa tagcttggta gtcttttgag
    36601 ctccactctt tgtaagggcg gtagcgaata gcttgtggta gatccatcaa aatctccttg
    36661 ttttatagtc gtcttcaaga atatactaaa cgttttctgt ttaaaaatca agaatttaaa
    36721 acatataaaa taaaaatatg ttaaacgctt gacataaaaa atattagtga taaaatgata
    36781 gtcaagaagt gaagcggatt cactagcgtt ggtcctacac attcttagcc acaaagctgg
    36841 cagaatggat gagggactaa caccaaatga aaaaggagac ttttaggcat ggataatcgt
    36901 cagattgcaa ctgaagtgat tgaggcttta gggggccgag aaaatgtgag gagtgttgcc
    36961 cactgtgcaa cccgccttcg agtaatggtt catgacgaag gaaagattga caaggaaaaa
    37021 gcagaagcta ttgacaaggt taaaggagct ttctttaatt ctggtcaata tcaaatgatt
    37081 tttggaactg gtaccgttaa taacatttat ggcgaagttg ttgctcttgg tttacccacg
    37141 tcatcaacca gtgagcaaaa agcagaagca ggcaaacaag gcaatatctt ccaacgggcc
    37201 attcgtacgt ttggagatgt ctttgttccc attattccag ctattgtagc aacgggtctc
    37261 tttatggggg ttcgtggttt ggtgacccag ccagctatta tggatttatt tggggtgcat
    37321 gagtacgggg aaaattttct catgtacact cgtattctaa cggatacagc ctttgtctac
    37381 ttgccagctt tggtggcttg gtcagccttt agggtctttg ggggtaatcc tattatcggt
    37441 attgttttag gcttgatgtt ggtgtccaat gaattgccaa acgcctgggt agttgcctct
    37501 ggtggagatg ttaagccgct aaccttcttt ggatttgttc ctgttgttgg ttatcaaggt
    37561 accgttttac ccgccttctt tgttggtctg gtaggagcta agttggagaa atggttgcac
    37621 aaaaaggttc cagaagcttt ggacttattg gtcacaccgt ttttaacgtt tgctattatg
    37681 agtatcttgg gactatttgt gattggacca gtcttccatt ctcttgaaaa ccttgttctg
    37741 gctgggacac aggccgtctt gcatttgccg tttggcattg ctggtcttat tgttggggga
    37801 atccaacaat taatcgtagt gactggtatt caccatatct ttaacttcct agaagcgcag
    37861 ctaattgcca ataccggaaa agatcctttc aatgcttacc taacagcagc aacagctgct
    37921 caagctggag ctaccttagc tgttgctgtt aaaaccaaat caccaaaatt aaaaggtttg
    37981 gcttttccat cgaccttatc agctctttta gggattactg aaccagctat ttttggggtc
    38041 aatctccgtt atccaaaagt ctttgtttca ggtcttattg gtggtgcctt aggtggttgg
    38101 gtcgctggac tctttggcat tgcaggaact ggttttggga ttacggtctt accaggaact
    38161 ctcctttact tgaatggcca attattgcaa taccttgtga ctatgcttgt cggtcttggg
    38221 ttagcctttg caattgctta tacttggggt tatcaagata gagaaaccct tccgttacca
    38281 gctgtcgaag ttgatcaaac ggctgatcag ccagccttag cagaggaaac tttatatagt
    38341 cctttgaatg gaacagttgt agatttagca gctgtttcag accctgtttt ttcatcaggt
    38401 gctatgggtc aaggcttagc aattaagcca gaagataata ccctatactc accagttgat
    38461 ggtaaagttg aaattgtctt tgaaacaggt catgcctatg ctataacgtc aagtcaagga
    38521 gcagaagtgt tacttcatat tggtattgat accgtgtcga tggcaggaga tggctttgaa
    38581 tctcttgtag cagtaggtca ggcggttaaa aaaggtgatc ttcttggaca ctttgatcct
    38641 agcaagattg cagaagctgg actagatgac accaccatga tgattgtgac caatagcgct
    38701 gactatcaaa gcgtggacat tcttgctcaa ggacatgttt tgattggtga tcaagtagca
    38761 cttatcaaat aaggacaaaa gagctaacta agcagctctt ttgtcctgcc atgaatggca
    38821 ggttggcaaa aaaatgagaa aagcctaaaa accttaaatt tgtgttattt tacctatact
    38881 ttgtaccttg ttttttttaa taaagtgttg ttatacttaa ggcgaactat aggaatgcgc
    38941 ttacatagat ggtatatcaa ctgggaagcc atgacttagt accaaaaata aggagtgtcc
    39001 aaatggataa acatttgttg gtaaaaagaa cactagggtg tgtttgtgct gcaacgttga
    39061 tgggagctgc cttagcgacc caccatgatt cactcaatac tgtaaaagcg gaggagaaga
    39121 ctgttcaggt tcagaaagaa ttatcttcta tagatagctt gcattatctg tcagagaata
    39181 gcaaaaaaga atttaaagag gagctttcaa aagaaaaagt accagaaaag gtcaaagaga
    39241 tattagcaaa agctcagcag gcagataaac aagctcaaga acttaccaaa atgaaaattc
    39301 ctgagaaaat accgatgaaa ccgttacatg gtcctctcta cggtggttac tttagaactt
    39361 ggcatgacaa aacatcagat ccaacagaaa aagacaaagt taactcgatg ggagagcttc
    39421 ctaaagaagt agatctagcc tttattttcc acgattggac aaaagattat agcctttttt
    39481 ggaaagaatt ggccaccaaa catgtgccaa agttaaacaa gcaagggaca cgtgtcattc
    39541 gtaccattcc atggcgtttc ctaactgggg gtgataacag tggtattgca gaagatacca
    39601 gtaaataccc aaatacacca gagggaaata aagctttagc caaagctatt gttgatgaat
    39661 atgtttataa atacaacctt gatggcttag atgtggatgt tgaacatgat agtattccaa
    39721 aagttgacaa aaaagaagat acagcaggcg tagaacgctc tattcaagtg tttgaagaaa
    39781 ttgggaaatt aattggacca aaaggtgctg ataaatcgcg tctgtttatt atggatagca
    39841 cctacatggc tgataaaaac ccattgattg agcgaggagc tccttatatt aatttattac
    39901 tggtacaggt ctatggttca caaggagaga aaggtggttg ggagcctgtt tctaatcgac
    39961 ctgaaaaaac aatggaagaa cgatggcaag gttatagcaa gtatattcgt cctgaacaat
    40021 acatgattgg tttttctttc tatgaggaaa gagcaggcag cggcaatctt tggtatgata
    40081 ttaatgtaga agatgagtct aatccaaata ttggtaaaga aattaaagga acgcgtgccg
    40141 aacggtatgc caaatggcaa cctaaaacag gtggggttaa gggaggaatc ttctcctatg
    40201 ctgtggatcg tgatggtgta gctcatccta aaaagaatgg ttataaaaat ccaaagttgg
    40261 ataatatagt aacatcagat tatagtgtct ccaaggcatt aaagacagtt atgctaaaag
    40321 ataagtcgta tgatctgatt gatgagaaag atttcccaga taaggctttg cgagaagctg
    40381 tgatggcgca ggttggaacc agaaaaggtg atttggaacg tttcaatggc acattacgat
    40441 tggataatcc agcgattcaa agtttagaag gtctaaataa atttaaaaaa ttagctcaat
    40501 tagacttgat tggcttatct cgcattacaa aactcgaccg ttctgtttta cccgctaata
    40561 tgaagccagg caaagatacc ttggaaacag ttcttgaaac ctataaaaaa gataacaaag
    40621 aagaacctgc tactatccca ccagtatctt tgaaggtttc tggtttaact ggtctgaaag
    40681 aattagattt gtcaggtttt gaccgtgaaa ccttggctgg tcttgatgcc gctactctaa
    40741 cgtctttaga aaaagttgat atttctggca acaaacttga tttggctcca ggaacagaaa
    40801 atcgacaaat ttttgatact atgctatcaa ctgtcagcaa tcatgctgga agcaatgaac
    40861 aaaccgtgaa atttgacaag caaaaaccaa ctgggcatta cccagatacc tatgggaaaa
    40921 ctagtctgcg cttaccagtg gcagagggaa acattgattt gcaaagccag cttttgtttg
    40981 ggactgtgac gaatcaagga accctaatca atagcgaagc agactataag gcttaccaaa
    41041 atcataaaat tgctggacgt agctttgttg attcaaacta tcattacaat aactttaaag
    41101 tttcttatga gaactatacc gttaaagtaa ctgattccac attgggaacc actactgaca
    41161 aaacgctagc aactgataaa gaagagacct ataaggttga cttctttagc ccagcagata
    41221 agacaaaagc tgttcatact gctaaagtga ttgttggtga cgaaaaaacc atgatggtta
    41281 atttggcagc aggcgcaaca gttattaaga gtgaaaatga tgaaaatgcc aaaaaagttt
    41341 ttaatggcat catggaatat aatcctttat cgtttaacaa taaatcttcg attatctttg
    41401 aaatgaaaga ccctagttta gcaaaatatt ggcgcctttt caatgatagc agtaaaggca
    41461 aagacgacta tatcaaagaa gcaaaacttg aagtctttac aggccagctg aatgctgaag
    41521 cagatgtgaa aacgagcttg gaaaaatcag atgattggca aactgtttca acctattcag
    41581 gacaagaaca ggtatttagc catgcactgg ataatattag tgctaagtac tggagaataa
    41641 cagttgacaa taagaaaaat caatatggat gtgtttcact ccctgaactc caaattttag
    41701 gttatccgtt acctaacgcc gacactatca tgaaaacagt aactgctgct aaagagttgt
    41761 ctcaacaaaa agataagttt tctcaaaaga tgcttgatga gttaaaaata aaagagatgg
    41821 ctttagaaac ttctttgaac agtaagattt ttgatgtaac tgctattaat gctaatgctg
    41881 gagttttgaa agattgtatt gagaaaaggc agctgctaaa aaaataaaca aagcaactct
    41941 cttagatagc aacattcaga ttaaattaac aaaatgtgac tatgataaag gtttgctgga
    42001 attgattaac caaaagacta aaaatctgag atgaatagtc ccagattttt agtcttttat
    42061 aggttttgat gacataaagc taaataatcg ttagactacc agaaagtggc gcttgtccgt
    42121 gagacatggc tgtcttgata agagcctgtg tcatcataaa gataaaaaag aacaagaaaa
    42181 aggagaaaac atgggaaaat tatatggaag tattgaagct ggtgggacaa agtttgtctg
    42241 tgccgtgggt gatgaggagt ttacagtggt ggataaaaca caatttccga ccaccacacc
    42301 agaagaaacc atcgcgcaaa ccatcgcata ttttaaaacc tttgaggccg atttggctgg
    42361 aatggcgatt ggttcttttg gaccaattga tattgatccg tcgtcagaga cttacggtta
    42421 tatcacaacc actcctaagt caggctgggc taatgtggat ttgttaggac aactttcggc
    42481 tgcttttaag attccttttg atgtgacaac agatgttaat agctctgctt atggtgaggt
    42541 gcttgctcgt cctggtgtgg aaagtttggt ctattacacc ataggtactg gtattggggc
    42601 gggagctatt caacatggcc actttattgg gggattaggt catacagaag cgggtcatac
    42661 ctatgtcatg ctccatcctg atgatatggc caagggattt ttaggtgttt gcccttttca
    42721 taaagggtgt ctggaaggga tggcagcagg tccaagtatt gaggccagaa cgggagtgag
    42781 aggtgaacgg cttgatcagg aagctgatgt ttgggacatt caagccttct acattgccca
    42841 agcagcgctg caagcaacca tgctttaccg tcctcaagcg attgtttttg gcggaggtgt
    42901 catggcgcaa gaacatatgg tgttgcgcgt gcatgataaa ttcactaccc ttttaagcgg
    42961 ttatttgcca gtccctgatt tgacagacta cattgttact ccagctgttg ttgataatgg
    43021 ctccgctact ctgggaaatt ttgctttggc caagttggct gcacaaggaa aatagggtct
    43081 atttttgcct gcttgtctag taaaagaaag cgcagtcagc tagaacatgc tataataaac
    43141 agagatcaac atcagaagcg aggaatatta tgtcagaacc attatttcta aaatcaacca
    43201 tgcacgacag gatttggggt ggcaccaagt tgagagatgt ctttgcttac aacatcccaa
    43261 gtgacaccac aggtgagtat tgggctattt ctgctcatcc taatggtgtt tcgacagtta
    43321 ctaatggacg ttatcaagga caacctttaa acacattata tgcgcaagag ccggccttgt
    43381 ttgggaatcc aaaggaagaa gtttttccac tgttaactaa gattttagat gctaatgact
    43441 ggctaagtgt ccaagttcat cctgatgatg cttatggaca tgaacatgaa ggagaattag
    43501 gcaagacaga atgctggtat attatttctg ccgaagaggg ttctgaaatt gtatacggcc
    43561 atcaggccaa gtccaaagaa gaccttcgtg ccatgattga agcaggcgct tgggatgatt
    43621 tgctgactcg tgtcccagtc aaagcaggcg attttttcta tgttccaagc ggcaccatgc
    43681 atgcgatcgg caagggaatt cttattttag aaacccagca gtcatctgat accacttatc
    43741 gggtttatga ttttgaccgt aaggacgtta atggtaatct tcgtgacctt catattgaaa
    43801 agtctattga tgtcttaacg attggtaagc cagaaaatag cgtgccagcc actatggttt
    43861 tggataatat ggtagcaaca actttagtct caacgccatt tttcactgtt tacaaatggg
    43921 taactagtca aatggttgac atgaaacaag ctgcgcctta tcttttagtg agtgttttaa
    43981 aaggtcaagg aaaactctat gtggaccaga aggcttatga gctagaaaaa ggcatgcact
    44041 ttatcttgcc taacgatgtt aaatcgtggt catttgatgg tcaattagaa atgattgtga
    44101 gtcaccccaa tcaactcgtt taataccatg gtatcaatat gagaaaaaag gataaaatta
    44161 tgactctctg tttctctgat aactttac
//
LOCUS       DAUCWO010000008        34975 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUCWO010000008 DAUCWO010000000
VERSION     DAUCWO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.008"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1277
                     /gene="rocA"
                     /locus_tag="VGY20_000406"
     CDS             <1..1277
                     /gene="rocA"
                     /locus_tag="VGY20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ4273293.1"
                     /translation="FVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFILDVVMLAQF
                     LFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYSVVKHNTLL
                     IYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYYLCVQGIDV
                     MSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEYRELINYSQ
                     HLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNAKDDRLDLT
                     CLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDLTKLLSFLLDNAI
                     EMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQSVFAIPALKKRDDWQFDLRN
                     VTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1378..4083)
                     /locus_tag="VGY20_000407"
     CDS             complement(1378..4083)
                     /locus_tag="VGY20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ4273294.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NILIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTREKYSSQFSEISWQGPDDSHILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLTNTASARIYLKQANTRVSRQLENITEPLATMAYDVTGTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAELSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLSKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPNHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPVSADKLLQA
                     EMEAVIDITERQSGRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPNAAFWKNPSNPQHQECFVSLFDGEKGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4098..5384)
                     /locus_tag="VGY20_000408"
     CDS             complement(4098..5384)
                     /locus_tag="VGY20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ4273295.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALVLQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5517..6557
                     /locus_tag="VGY20_000409"
     CDS             5517..6557
                     /locus_tag="VGY20_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4273296.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6608..8302
                     /locus_tag="VGY20_000410"
     CDS             6608..8302
                     /locus_tag="VGY20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ4273297.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDTPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFPMRGIIEGFYGTPWTREERIDCLRFIGNKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLVVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERANTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8327..9769
                     /locus_tag="VGY20_000411"
     CDS             8327..9769
                     /locus_tag="VGY20_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4273298.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQVYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADTAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEEMALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10380..11258)
                     /locus_tag="VGY20_000412"
     CDS             complement(10380..11258)
                     /locus_tag="VGY20_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4273299.1"
                     /translation="MSLLCLDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAVNKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVIGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALTEQGDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11455..12405
                     /locus_tag="VGY20_000413"
     CDS             11455..12405
                     /locus_tag="VGY20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4273300.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12415..13341
                     /locus_tag="VGY20_000414"
     CDS             12415..13341
                     /locus_tag="VGY20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ4273301.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13422..14867
                     /locus_tag="VGY20_000415"
     CDS             13422..14867
                     /locus_tag="VGY20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4273302.1"
                     /translation="MKKWQKIVCVTGTVLSASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15173..15778
                     /locus_tag="VGY20_000416"
     CDS             15173..15778
                     /locus_tag="VGY20_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ4273303.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15768..17492
                     /locus_tag="VGY20_000417"
     CDS             15768..17492
                     /locus_tag="VGY20_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4273304.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRNDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17492..18976
                     /locus_tag="VGY20_000418"
     CDS             17492..18976
                     /locus_tag="VGY20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4273305.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18906..22412
                     /locus_tag="VGY20_000419"
     CDS             18906..22412
                     /locus_tag="VGY20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ4273306.1"
                     /translation="MVYRQKSSEIVTRTITKPSRAISNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLKQPYTRNAEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFSLNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYASGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22550..23428
                     /locus_tag="VGY20_000420"
     CDS             22550..23428
                     /locus_tag="VGY20_000420"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ4273307.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23680..24435
                     /locus_tag="VGY20_000421"
     CDS             23680..24435
                     /locus_tag="VGY20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ4273308.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLEEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24488..24826
                     /locus_tag="VGY20_000422"
     CDS             24488..24826
                     /locus_tag="VGY20_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ4273309.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            24828..25133
                     /locus_tag="VGY20_000423"
     CDS             24828..25133
                     /locus_tag="VGY20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ4273310.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(25569..26642)
                     /locus_tag="VGY20_000424"
     CDS             complement(25569..26642)
                     /locus_tag="VGY20_000424"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ4273311.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLKRVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AVIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26698..27450)
                     /locus_tag="VGY20_000425"
     CDS             complement(26698..27450)
                     /locus_tag="VGY20_000425"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ4273312.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKILSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27691..27761)
                     /locus_tag="VGY20_000426"
     tRNA            complement(27691..27761)
                     /locus_tag="VGY20_000426"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27727..27729),aa:Thr,seq:ggt)
     gene            27928..28182
                     /locus_tag="VGY20_000427"
     CDS             27928..28182
                     /locus_tag="VGY20_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ4273313.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            28203..28598
                     /locus_tag="VGY20_000428"
     CDS             28203..28598
                     /locus_tag="VGY20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ4273314.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVGNLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            28883..29202
                     /locus_tag="VGY20_000429"
                     /pseudo
     CDS             28883..29202
                     /locus_tag="VGY20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29199..29762
                     /locus_tag="VGY20_000430"
     CDS             29199..29762
                     /locus_tag="VGY20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4273315.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            29764..32412
                     /locus_tag="VGY20_000431"
     CDS             29764..32412
                     /locus_tag="VGY20_000431"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ4273316.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAAVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFLANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            32566..34973
                     /gene="cas3"
                     /locus_tag="VGY20_000432"
                     /pseudo
     CDS             32566..34973
                     /gene="cas3"
                     /locus_tag="VGY20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        10366 a         6679 c         7606 g        10324 t
ORIGIN      
        1 gctttgtaag tgcaattccg cttcgcctga aaaatatttt ttatttatca cttagtatgg
       61 ttttgtttca agtagttttt tgggctttct ttccagacca tttcatcctt gatgttgtga
      121 tgttggctca gtttcttttc tttgcgctta tagccctata ctatggtaaa tcaataaaag
      181 ctaagttttt aatgttttat gccttttttc cactagtgtc tattagtttg gttaagcgat
      241 ttattgtatt ttttgttatg ccattatttg gtatgcctta ttcagttgtt aaacataata
      301 cactactgat ttacagcatt acttgcttta gtattttttt gatttatcgc tgcattcaag
      361 tttttcattt cgatttttca acctggcgcc aatattttca atcacataga gctagtaaac
      421 tattagtgtt tactaactct tcgatggctc tatattactt gtgtgtccaa gggatagatg
      481 tgatgtcgcc ttctttatca ggacttgcta caacgactgc tcgttcaatc atagtgctct
      541 tttatttcat tttgtttctt actttattaa ttcatttaga gcgttatgta aaacaaaact
      601 ctattgaggc aattgtgcaa caaaaagaat atcgtgagct gattaattat agtcagcacc
      661 ttggattgct gtatcaagat attcaagagc ttaggcagct attaactacc gtgtccagtc
      721 gtcttaagat tggaatcgaa caaaatgata tctctattgt gagacttact tatgaaggta
      781 tcttgaatgc tgaaaagaat aatgctaaag atgacagact tgatttaact tgtttagata
      841 aattacaagt tgaagcaatc agacatattg ttttagctaa attaattgag gcaaaaaata
      901 agaagcttaa ggttgaggta tcgatcccta attgtattgc aacgtttttt ctagaagtag
      961 tagatctcac taagctattg tcatttttgt tagataatgc tatagaaatg agtttggaga
     1021 caaagcaacc ttgcttatca atagcatttc tggatcaaaa ccataaactt gtcatagtca
     1081 ttcaaagcag tactaaacaa ggacaagatg atagtcaaag cgtgtttgct ataccggctt
     1141 tgaaaaaaag agatgactgg caatttgact taaggaatgt cacgaccatt ttaaatcgtt
     1201 atgactatct cacaattagc tcgcagattc atgatggcat tttaacccag ttaatagaaa
     1261 tagctaagcc tgactgatgt taaaggtatg aataatcact caaaatcaac ttaagagtct
     1321 gagacaatag tcccagactc tttttgaata tgttaatcta ttactaatga taatagttta
     1381 ttgtttcttc caagctagag ttaaaatttc cgctttgcca agttggctgg gtgtgctttg
     1441 cttgctgtct ttttctaaca aattagtgac cttggcattg tagtttggca ggcttaggct
     1501 aaagtcacaa gttttatcgt ttgagagatt atagctgcgc gtgataaggg cattgtctgc
     1561 taagcgacgt ttgaaagccg ttagagcgac ttgatcgtct gccccagtga catagctatg
     1621 ctctgctctt aagcttccct tatgatggct ggtttgttcg actaggatag gaacttggcc
     1681 ttcttgtgcc cgccaatagc tggcaaattg tgtttgctta gtgatgcttt caaaactata
     1741 agataggctg tgtttgccga ggcattgggc ttcaggagta gggaaatacc cccagtcgcc
     1801 catttcacca acagaacgta agagagtgat ggcaatggtg ttggtatctg gtaagatctc
     1861 atattcgttg aggccgtagt taccaatagt gacccccttt tcaccatcaa agagactcac
     1921 aaagcattct tggtgctgtg ggttacttgg attcttccaa aaggcagcat ttggatgatt
     1981 tggacgtttg acagtttcaa aaatactgtc agctaggtga tggtctgtct taaggtgcgt
     2041 tgggaataag acgcgcaagc gatgattagt catttggtta tcaaaacttg tggtgaattg
     2101 gaggcgagga ttgtttttct ccatgcggat aagggttgtt aaggtcagtt cagctttttc
     2161 ttgtgaacgt cctgattggc gttccgtgat gtcaatgaca gcctccattt cagcctgtaa
     2221 gagcttatct gcggagacag gaatggcaaa ggtttgctgg atttcaagtt cagcaacttg
     2281 agcggtgttg ctaatgatgt taagcttggt ggtcccttgg tctgcataga aaggttggtc
     2341 atgatttggt tggcgggaaa tgtattcatt tccaatatcg ccacaatctt caaaccgcaa
     2401 caggtcttga tagattagtc cagattgttt atcggtgatg gttagacgac catcggtttg
     2461 tatcataact tttagaaact cattttcaag gcactgatta ctatcgtcgt agagtggaac
     2521 cgttgctgaa ggagctgtct cgtttcctag ttgtaatgct aaggctttcc aagacatggc
     2581 agggagagtt attggtagcc gtaatctcac tttgatggcg aaataaggtt ctcggaaggt
     2641 tctcttggac aaatcgtagt caaaggcgat gcttgtgtcc aaaatgtctg cttctgagat
     2701 agtgtttcct gatacatcaa tgacttggaa agattgggag agttctgcca aatactcttg
     2761 agcttcttgg taaacctctt taggaaagcg ttggccaaaa taatattttt tccaggtcag
     2821 ggtgagctca gtaacacttg ttttggaatg gccactggta ttaaataaga ggaaaggttg
     2881 gctatctgtt ggaaagtcac tggtatcaat ggcgtcagca atttgcttag cagcttcttt
     2941 tgctaaataa tgtccgactt cataggcttt ttcaaagcgc gtcatcattt cccgatgaac
     3001 gctatcaaca ctacaaccac agatagaatc atgagggtga ttttgcatga gggttttcca
     3061 agcgtaacgc agttggtcgt gagggtaggt acctgttaca tcataagcca ttgttgctaa
     3121 gggttcggtg atgttttcga gttggcgaga gactctggta ttagcctgtt tgaggtaaat
     3181 gcgagcagaa gctgtgttag ttagggtata ccagccatcg gtttcttgac tggtaatctc
     3241 tccttggacg gttgaaagat tctctggtaa atcatctgcg agatcagcca agtaatcttc
     3301 aaagcaggaa tgcacaaatt cgtagtcagg atagagttgg ttggctaagg cgattgcctt
     3361 ggtgacatca agttgtacgg gttgatgatc acacccgttc atcatcagaa ggtgcttggt
     3421 tgaggcaaag cgttcagcgt cagcaagttt tttatcccaa aaaagacgcg cctcagcttc
     3481 tgttgtcggg atctcattgc cattgctgta ccagttggcg aaaaggagtc caagaatatg
     3541 gctgtcatct ggtccttgcc aactgatttc agagaattgg gagctgtatt tttcacgggt
     3601 atccacctga ttgttaaatc cagttggacg aatgccacga ccaaaggcag cagcttgtag
     3661 gccggctttt aacatcagct gtggtgtttg tcccatattt ccaaaggtat caggaaaata
     3721 accaagtggc acactagcgc cccatctgtc acaatcttcc ttaccaatca gtatattgcg
     3781 cacattggac tcgctgctgg tcaaaaaatc gtcctgtaag atatagaaag gtccgatacg
     3841 gagttttccc gaagcaatgg cttgtctaat ctcaggttct cgttcggggc gtacttttaa
     3901 ataatcatct aggataatgg tttgcccatc caaatgaaaa ctatgaaaat caggatccgt
     3961 ttgaaaaact tctaacaggt catctattaa gttaatcaga cgcatgtggt gttgttcgta
     4021 agccatgtac cactcgcgat cccagtgact gtgtgaaata atatgtactt ttttagttgc
     4081 catagtggcc tcactttcta aggagcaata ctaaagccca gatagtcaag aagtagttca
     4141 cagaacatca tgttagccca agagaaccac tcacgagtgt attgataagg atcattgaca
     4201 tcgaaaccct cgtgcattaa gtgggtacca gcatctgttg ccacaagcaa atcaagcatc
     4261 tcttttttac tgtcttggtc aagagcggtc aatccttgta agacaagggc gatatgccag
     4321 atataatttt ctggtgtatg ggaagaaccg atgcccgctg ctgcatttcc ctgataataa
     4381 taagggtttt cttggctaag aatggtgcgg cgcgttgcca aatagatagg gtcatcttta
     4441 gcgcaaaaac caaggtaagg tgcagcaagg aggcttggaa tattagcatc atccatgatg
     4501 gaaaactgtc ccagaccatc cacttcataa gcgtaaatgg tttctccctt gctgttttga
     4561 acaagggcat ggtctgcaat acccgattga atagcttctt tcagctgctt ggcacgagta
     4621 gctactggtt cattgtgaaa aaggttgtta tataaatctt ctagatagga aagcacaacc
     4681 actgcaaaca tatttgaagg aatgaggtaa ccatattgac aagcatcatc acttggtcta
     4741 aagcctgacc aggtcatccc tgttggtgcg acaggacttc cttttccagc atgtgttagg
     4801 gtatcttctt tgcgccaagt atcgcgttca aacaagtaag gagactgggc gtggtcttgc
     4861 tcaactgtcc ataggtcaag aatgagttct accccacgat gaaaatcgtc attaaactga
     4921 tcggtgcttc ctgtttcacg atagagcaag taagctagct gaatagggta gcagagggaa
     4981 tcaatctcat atttgcgttc ccaaatccaa gggttcattt gggtacgatc tgtttggtgt
     5041 ccttttccgt tagcttcttc gttgaaggca ttggcatagg gatcgataca aatgtagcgg
     5101 aattggcgtt tgaccaaacc ggcaataatc tttctaattt cttcatcttc cttggcgagg
     5161 aagaggtaag gtttcatttg cgcagtggaa tcacgtaacc acatggcagg aatatctcct
     5221 gtcaagagaa agctagtgcc atcttcgtag gtttttaggg ttgtctctaa ggtattggta
     5281 aagctggttt tgaaattaag agcccaatca gggtgttggt ctccacaaag gctttccacc
     5341 ttggccataa attggctgac gctagttggg attggtcgtg tcatatcata tcctccaaaa
     5401 agatatatca tttatcttcg gtttcattat aaatgataag ttaaactttg tctataagtt
     5461 tttgaaaagg tttacaactc ttttttgaca tgctatactg ataacgggag taaagaatga
     5521 caaaattatt atacaaaaag atatatcatt tcattaaaga gcagatagca tcaggtcgtt
     5581 tgcaaattgg tgaccgccta cctactgaaa aagaattgtc agaacagttc tcggttagcc
     5641 gtattacttc taagcgagcg cttgtagaac tggagcaaga aggcttgatt acgagaagtc
     5701 gtgggaaagg aagcttcgta gctgaaaagc aagtgacgtc ccctgaagcg aataaggatt
     5761 tattgttgat tcttcctttt gcttctgatt atgaattggg agattatgct aagggaatta
     5821 tgacctccat tgctgaaaca ggttaccgtc tcatggttca attggccagt acagtaaggt
     5881 tagacaccct gtcagactat gctgggatta tttattatcc tgaagatgtc aatcacagta
     5941 ttgatttcct gttttactgc gaccatcatc acattcctct agtcttgttg gataaatctc
     6001 tagatctttt tcagtttccg tctgttgtag cggataataa aggcggtgcc tgtcaattga
     6061 ctcagcactt gattgagcaa ggctgtgacc agattagttt tgtggcaaca ggctcttttg
     6121 gagaagtatc atccgttaga gaccgttatt tgggttactt agcggcaatg gcagaatcat
     6181 ctctaaaatc ccattatttt cctaagacag agactgaaac aacagatgct tatttggacc
     6241 gtttggtgaa aaccttatcg gaaacgacag ccactaaaag ggggcttgtc gtggaaaacg
     6301 attggttggc tattcagctg attcaaaaaa tcacacaggt aggcttatct attcctgatc
     6361 aagtcgctat tgtaggcttt gataatagtc aagcctcccg tttgttgagt cccaaattaa
     6421 gcacagcagc tcaagatttt taccaaatgg ggcaagaagc agctaaagtc ttgttgcaga
     6481 ttattcatga gccagaccaa gcagtggtgt cttgccagtt acctgttcaa gtatttatca
     6541 gagaaagtag tcactaaaaa agacgttact agcatgagct ggttagctca ggtaaaggag
     6601 accagaaatg accatttatc acgccttaaa ggattatcaa gaagtcatga caagaggaga
     6661 ctatcttgtc tttgacacgc cattgacttg ccgatttatt ggccgttttt tccgctttga
     6721 aaatcaaaaa gccctgctgg cagaattggc aacgtcaaag tattttcagt ggattgaaga
     6781 aggccaagca gaggtgacga tgaaacactt ttttaaccgt cagctggcta aggatgcctt
     6841 taccctgaag gtttcagaag ataaggaaat cattattgaa agccaaaacc ttagaggttt
     6901 tcgttacgcc caagaagctt tgcttaaagt catgaccttc aaaggggata aactttattt
     6961 gcccattgtt tctgtcaagc acagcccgtc atttcctatg cgggggataa ttgaaggctt
     7021 ttatggaaca ccgtggacac gagaggaacg aatcgattgc ctacgtttta ttggaaataa
     7081 aaggatgaat acttacatgt atgctccgaa agatgatgat taccagcgaa aactgtggcg
     7141 cgatctttat cctgaggatt gggtgactta tttcaaagag ctacttgtag tagccaagga
     7201 agaagggatt gatttttggt atatgattag tccgggactt gattttgatt ataccaagga
     7261 agctgactat cagttgctct atcaaaaatt gcaacaactt ctagcgttag gtgtttgtca
     7321 ttttgggctt ttgctggatg atattgacta ccaaattgta gatgctgttg agagacgatt
     7381 caaaaagact gcctatgccc aggcccactt ggcaactgag gttcatcact ttttaaacca
     7441 acaacatgcg gcgccagaat tggtgatctg tccgacagaa tacgacaacc accatgattc
     7501 tatctatttg caagagctgt cagaaaggat tcctaaagaa gtcgcctttt tctggacagg
     7561 cccaagcacg ctagcgagtc aaatctcgca agcagatatc gaaaccatgg cagcagtata
     7621 tcagcggcct atcattattt gggacaatat tcctgttaat gattatcaaa aagatccaga
     7681 acgtctcttt ttaacccctt ttgctaatcg ctcaccattt ttatgccaat cagactatca
     7741 ggtcaagggg attgtttcta accctatgat tagttgggaa ttatctaagc tgaccttgac
     7801 agacatgagc cattatctgt gggatgctaa ccgttatcag cctagtaact cttggctaga
     7861 aaccttaaca gactatacag gagatgctga attagcctta gctttacagg catttgcttg
     7921 gcataatggc aatcgtcatc tgcacaggga ccttcctttt gaagtggaag aagctctcct
     7981 ggctaaggat gtatcaacgt tatcagcttg ggtagatgaa ttggtagaac gtgccaatac
     8041 gttgagaaag ctagataagc cagcgtttca acaagctatt gttccttggt ttgaacgtgt
     8101 ggccaaagac caagattttt ggcaggccat tttagagcag gggcctcatt tggaaacact
     8161 ctatgctgac ttacaagaag ataaacaccg tattggaagt gacattccaa gtcgctacta
     8221 tcgcatctac tatcaacagc aagataaact aacagctaat caaggtcaag ttacccaagc
     8281 aagacctgaa gattatgctt agtcatacga gctagaaagg aatgatatgt tagcatttcc
     8341 aaaagagttt tggtggggag gagccacttc aggccctcaa agtgaggggc gatttgctaa
     8401 acagcaccgc aatctctttg attattggta tgaagaggag cctgacttgt tttacgatta
     8461 cgtgggacct gatacggctt cggatgccta tcatcagatt gaatctgatt tggccttgtt
     8521 agcttcacta ggtcacaatt cctaccgcac ctccattcag tggacacgtt tgattgatga
     8581 ttttgagcaa gcgacgatta atcctgatgg ccttgcttat tataatcgag tgattgatgc
     8641 ttgcttggcc aatggtattc gtcctgttat taatttgcat cattttgacc tgccaattgc
     8701 cctctatcaa gtctatggtg gctgggaatc aaagcatgtc gtggacttat ttgtcgcctt
     8761 tagcaaggtt tgttttgagc agtttggtga tcgggttaag gattggtttg tccacaatga
     8821 acctatggtc gttgtggaag gctcttatct aatgcaattt cattaccctg ctatcgtgga
     8881 tgggaaaaaa gcagtccagg tcgcctataa tctagcccta gccacagcca aggttattca
     8941 agcttacagg cgaggtccag cagaacttag tgatgggcgt attggaacca ttctcaatct
     9001 gacgccagcc tacccagcta gtcaatcaga agcggatacg gctgctgcgc attttgcaga
     9061 actttggaac aatgacttgt ttatggaagc tgctgttcat gggaaattcc cagaagaatt
     9121 ggttgcggta ttgaaaaaag atggggttct gtggcaatca acgccagaag aaatggctct
     9181 tattgctgag aatagagtgg attatttggg attgaatttt taccatccta agcgcgtgaa
     9241 ggctcctgat gccattccag tgattagccc atcttggagt cctgaatggt attatgaccc
     9301 ctacctgatg cctggccgcc gcatgaacgt ggataagggg tgggagatct atcctgaggc
     9361 tgtctatgat attgccatca agatgcgaga ccattacgac aatattcctt ggttcttatc
     9421 tgaaaatggt gtagggattt caggagaaga ccgttaccgt gatgaaactg gtcagattca
     9481 agacgattat cgcatccaat ttttgaaaga acatttaacc tatctccaca aaggaataga
     9541 agcaggctcg aattgttttg gataccatgt atggacgcca attgatggct ggtcttggct
     9601 caatgcctat aaaaaccgct atggccttgt ggaaaataat atccataccc aagtgcgacg
     9661 acctaaagct tcagcttatt ggtttaaaaa agtagctact cataaccgct tgatttcatt
     9721 agaagtaatg gaggagtttg gaggctctgc ttctcacctc agtgattgaa cgtgtgaacg
     9781 tctcctttga gacaagtgaa taatgatgtt accaatgatt aaacaagggc ctttttcctc
     9841 atgttaagag gatcgaggct cttgtatttc tttggaaggt gttggtattt gccgaactag
     9901 atataagcgt tctgtctggt ttggcatcat ctatccatct tgggtaattt ggctcttcaa
     9961 ttctttagag tatcttggct aaggatgctt gtttttcatc ttggtatttt aggattttct
    10021 ttcatatttt tggaccagat aaaagcccct catggtttct agtgactgtt tgttatgtca
    10081 ctgtccacga taaggggact atatgagaga gttattaagt gaggctttgc ttgttggcga
    10141 taactagcat agcttctaat agctcgagta ttaactgatg accaatgcct ttgacagaag
    10201 cgtcagcaac aacgacatca acaaagggac caggcatcag gctgcgttca tcagcagaga
    10261 gtcatttagg cagaaagcca gtatcgttgt agctaatcag caatcctttt aggatgtctt
    10321 tgtagatgtc agaggcgatt agggacaata ggggataaga gggattaagc ggtccatggt
    10381 taggcctcct tgtgttcttg gtaaaaatca atggcagctc caagaagatt ggcttcgttt
    10441 ttaaagtgac aaatggctaa ttggggtcta aggtcggaga tttcgacggc ttggtagagt
    10501 ttgtctagtt cagcttcaat cgctgacagt agaaagtcag cttgggaaac accacccccg
    10561 attaaaatca gttgaggatc aaaggcgtgt tgaatgttaa aaataccgat ggctaaactt
    10621 tggagaaaga cctgcctttc tttgagtgct aggggatctc cttgttctgt gagggcaagg
    10681 acagctttgc cagtataatc ttttccgtta ttgacgattg cactatagcg cttggccata
    10741 ttcacaacag tgcctaattg actaaaggtt tggtagcggt cgttcatgat catgaaacca
    10801 aattcgcccc caaataagtg ggcaccatgg taaatcttgc catcaatgac caagcttcct
    10861 ccaactccag taccaataac gagcatggct aggctactag cgccttttcc tgcccctaag
    10921 gtagcttcag ctaaggcggc acaattggca tcattttcga tagaaatagg gtaatgtagc
    10981 cgttcttcta aggcctcttg tattttaaaa tggtgaatgt agggaatagc gctggcgcct
    11041 tcgataaccc ccttttcttt attgactgcc ccaggtgaac tgatggcaat acctgagaaa
    11101 tggtaagcag agtaacgagc aacttcttgg tcgagtaatt ggtaaaactt ttccagacta
    11161 gagggagtgg gaaagctgct ttgctggctt aattggccat tgtgacagag ggcaaattta
    11221 agggaagtgc ctcctatatc aagacataac aagctcatga ggtgttctcc tttattgata
    11281 gtagaataga cgatcatgtc tcatcttagt atgacagatt taagcaacaa tgaaaaggct
    11341 ttctaagtga ttctatactt tgtgttatca tacctataat ttgttgcttt caaataatga
    11401 aagcgcttga tatactgtaa ctataacgtg attaacacaa agggaggttg ggaaatgaca
    11461 agacaaacca acaaaaagac atccttttgg aaaaatgtga ttaaataccg ggccttatta
    11521 ttaatggtat taccaggttt catctggttc atctttttct tttatattcc agttttggcc
    11581 aatgtggttg cttttaaaga ttttcattat tcagcagggg gctttatgga aagtttgaag
    11641 gagagccctt gggttggttt ggctaacttc aaatacctct ttgcttccaa agatgcttgg
    11701 ttgattacca gaaataccat cgcttacaat gtgattttct tactctttaa tgttttcttt
    11761 gcgattgctt ttgccattat catgagtgag ttacgaaata agcgaacggt taaggtctat
    11821 cacaccatgt ccttattgcc ttatttctta tcatgggtgg ttattgaata ctttgtttct
    11881 gcctttttaa atacggataa aggtttcatt aatcagctct tgactggaag tggagcagat
    11941 ccaatcaagt ggtattccaa tcccacttgg tggcccttga ttttactctt tatgagtgtc
    12001 tggaaagggc taggctacaa tagcattatc tactatgctt cagtcaaagg gatttctgat
    12061 acttattatg aagctgctat ggtggatggt gcaagcaaat ggcaacaaat taggaacatc
    12121 accattccac aattattgcc aatgatgtct atcctattga tcattaatat tggtaatatc
    12181 tttaaatctg atttcggact tttctacgtt attcccaaaa attcaggccc actttatgac
    12241 gtgaccagtg tgttagatac ctatgtctac aatgccttga ctgctacagg agatattggg
    12301 atggcatcgg cagctagcct ttatcagtca gtagttggga ccagcatctt gttagtcacc
    12361 aatgcgattg ttcgtcgcat ggaccctgat gcagccttgt tttaggagga aaacatggag
    12421 aaaaagaaaa aagtcgaaaa ggttaatgtt agaacctttg atcgaaaaac caatgctatc
    12481 tttaatattc tgattggtct gtttgccatc tcttgtatca ttccttttat cttcgtgatt
    12541 attatttcct tcactgatga aagttacttg attaatcatg ggtatagttt ttttccagat
    12601 gtctggtcaa ctaaggctta ccagtatatt tttcaaggag ccatgtccca tagaatcatg
    12661 aggtcatttg ggatatccgt gtttattaca gtggtgggaa cctttattaa cacgaccatg
    12721 acatcaacct atgcttatgc gatttcaaga ccttatttcc catacagacg tttttttact
    12781 gtttatgcac ttatcaccat gctctttgca ccagggatgg ttgctaatta cttggtggtc
    12841 agcaatctcc ttcatttgaa ggatacggtt tgggctttga ttttaccaat ggctctgggc
    12901 ccatttggca tcttggtcat gagaacgttc tttaaaaaga cagttccaga tagcattatt
    12961 gagtcggctc gtatggatgg ggctagtgaa tggatgattt ttatgaaaat tgttctccct
    13021 ttggctgttc cagggattgc taccatcagt ttattttctg ccttaactta ttggaatgat
    13081 tggtttaacg ccttgcttta tgtgcaaagt gaaaacctct acccaatgca gtacttactg
    13141 atgaaaattc agagcaacct acaggccttg gcacaaaatg ctggcatgag tgcacaaatg
    13201 gcagatagct tagcatcgct gccaaaagaa tcagttcgta tggctatcgt ggttattgca
    13261 accttgccga ttgccttgac ttacccattc ttccaaaagt actttgtcgg tggtttgacc
    13321 attggtgggg tgaaggaata ggacttaagg aactggtcac aagaccagat tttatctaat
    13381 cattagtaac aaaagaaaac attttttagg aggcttcact tatgaaaaag tggcaaaaaa
    13441 tcgtgtgtgt gactggaact gtgctttcag cgtctagttt agcagcctgc gaaagcaagt
    13501 cagcatcaaa ggatagtgat gtcaaattat tgatgtacca agttggtgac aaacctgata
    13561 acttcgatga attgatgaca attgctaaca agcgcatcaa agaaaaaaca ggtgcaacgg
    13621 ttgaccttca atacatcggt tggggggact gggatgataa aatgagtacc atcattgcct
    13681 ctggtgaaaa ctacgacatt gcttttgcca ataattatgt ggtcaatgca caaaaaggtg
    13741 cttttgctga tttgacaacg ttaatgccaa aatacgctaa gaaaacgtat aaaaacttag
    13801 acccagccta tattaaagga aatactattg acggtaaact ctatgccttc ccagtagatg
    13861 ccaacgttta tgcccaacag atgctttctt tcaataaaga actagtggac aaatatggcc
    13921 ttgacatttc aaacattaag tcctatgcag atgctgaaaa tgtcttgaaa caattccacg
    13981 aaaaagaacc aaatacagca gcttttgcta ttggtcaagt ctttagtatg tcaggtgact
    14041 atgactaccc attaaccaaa acccaaccct ttgctgtgaa aattgatgaa ggcaagccaa
    14101 ccatcattaa tcagtatgaa gatgagtcct ttaaaaacaa tctccgcttg atgcacaaat
    14161 ggtataaaga aggtttgatt ccaacagatg cagcgaccaa tacagaaggt tatccccttg
    14221 aaggaaacac ttggtttatg cgtgaagaaa cccaaggtcc tatggactat ggcgatacta
    14281 tcttgaccaa tgctgcagga aaagacatcg tgtctcgtcc attgactaaa ccgctaaaaa
    14341 ccacatcaca agcacaaatg gcaaactttg tggtatcaag cgtatctaaa aacaaagaaa
    14401 aagcagttga agtcctttct cttcttaata gcgatcccga attgttaaat gggcttgttt
    14461 atggtgtcga aggcaaagct tgggaaaaaa ttggcgacaa gaaaatcaaa ctgctcgatg
    14521 gctatcaacc aaaaatgcac atgggtgctt ggaatactgg taacaataaa atcctttaca
    14581 ctcaagaatc tgttactgac gacatgattg ctaaacgtga ccaatcaatc aaggatgcta
    14641 aagaatctcc aatccttggt ttcactgttg acacaaaagt tatcaaaacg gaattgtcaa
    14701 atatttcaaa tgtcatgaac cgatataaag caagtatcaa tactggtaca gtggatcctg
    14761 acgaagccct tccaaaactt cttgcagacc tcaaaggtgc tggttgggac aaggtgcaaa
    14821 aagaagttca aaaacaattg gacgactttg tagctaaaga caagtaaggc attcattttc
    14881 ttgattataa gttgagggct gggaaatctc agctcttttc ttctgatgag tggaaaaaac
    14941 ggatcatcaa ataagtggaa gttataaagg gagaaaaatt aatgtaaata atgttactac
    15001 tcttagtaaa gcggtttcca aaagcctata aagtgtgcta tattagaaga gtctaaacta
    15061 ttaatcagag taaaactaat accagcatct tactgggggt gctaaaacta gtctcgtagc
    15121 tgctggtcct tgaaaccgct ataagtgtta acaagaaaaa gaggactatc atatgaatgg
    15181 cattgaaaaa acctgctttt taatttggaa aatgatccaa ttgaccctca tttttcatct
    15241 cttgtcctta gctggactga ttgtgtttgg ggtggggcca gcctggcaaa ccattgtcac
    15301 cctattttta aaaacacctc aagaagaaaa ccactattct ttgaaaaggg ctgttcaact
    15361 ttggaaatct tatttcaaag aagctaatct aagatttggt ctttttgccc tgactttcct
    15421 ttttttaacc tttaatttgc attgggcagt acagtttcca tccttgtttt ggtttaccgt
    15481 gtctttcttg attgtaatag ccatggtttg gctaaccatt acctatcttt acatggtttt
    15541 ttatgcagtg agctatgaga ttaacctttg gaataatatg aaattagcct ttatcagcgt
    15601 ctttttaagt ttcaaatcct ttttactcat gctgagtgtt ttgctgggaa ttaccttagt
    15661 gacttggcaa tacaaaggcc tttatctctt tttaaccttc ggagctctag tcttttgctt
    15721 ggactttgtc actaaggcta atcgtcgact agtagatgga gtgattgatg agtgttaaaa
    15781 cattgggaag gtatgtgact aaccacttta agcagggttt tttgattaat cgactgatga
    15841 aattatatag cctgctaatt gtaagttttt tcactttagc agctattggc ttgagcagtt
    15901 attctattag tagtacttat aaaagggtgg atgctgaggc gcagatgcga ctggaggaga
    15961 ctctaggtag attgcagtta caaaacgata ttaccctacg ggttttagac cagttggttg
    16021 gccgttcaga agattaccat aacctctatc aatatatgac gttaacccca aaccaatatt
    16081 tttcaaacgt ttttgaagac tgggaaaaag gaaaagacac ggtcctattt tctgaagagg
    16141 tgaggcgtct ttttgacttg taccctgatg tgacttctat gaccctactg ctagaggaca
    16201 gtagtgctta tctgtacgcc gatagggtca ttaagacggg tcgtttgctt tatagcaagc
    16261 ctgaaaaagt aaccggaaat gttttagttc gctccattcg aaatccagaa tcaggagatg
    16321 tgacaggtcg tctttacctg acctttgaca aaccaccgac tttggtagag acacagcagg
    16381 atcactattt agctactttt gcttttgatt actatggccg caaactcttc catcaaggag
    16441 gtcggagatt tcctcattta gaggctgagg ttaaaaaggc tatcaaggca gatcgggcag
    16501 ctgatcttag taatctcagc aaggcttatc ggatgcagta caatcgctcg ggagacctct
    16561 tagcttatgt ggctgtcaga aaatcctatt tattagcgga agctgtcagg acagtttttg
    16621 tgtatggcct tgtttctcta cttttagctt ggctgttatt gcagttgctc tttagggttt
    16681 tccgaaatta tatccagcag gtttctgaaa ttacggatac tgttgaaatg gttgcagcag
    16741 gcgatttgtc tttaaccatc gacaacagcc acatggaatt ggaactgtat catatctcag
    16801 aagccattaa tcagatgttg gccagcatta aagcttatat tgacgaagtt tatgtgttag
    16861 aggtagagca acgagatgcc caaatgagag ctctgcagtc tcaaatcaac cctcattttt
    16921 tatacaacac gttagagtat attcggatgt acgcccttag ttgtcaacaa gaagaattag
    16981 cagatgtcat ttatgccttt gcgagtctgc ttcgcaacaa tattagccaa gataagatga
    17041 ccaccttaaa agaagaactg gctttttgtg aaaaatacat ttatctctac caaatgcggt
    17101 atccagatag cttcgcttat catgtaaaga ttgatgagag tattgctgac ttagccattc
    17161 ctaaatttgt cattcaacct ctcgttgaaa attattttgt acatgggatt gattatagtc
    17221 gcaatgacaa tgcactaagc atcaaggcat tagatgagac ggatcatctc ttgattcagg
    17281 tgcttgataa tggacgtggt attagtcaag agcgcttagc agatatggaa aaaaggcttc
    17341 aagagcacca aacaacaggc aatagctcta ttggtttgca aaatgtttac cttcgtcttt
    17401 tccatcattt tcgagacagg gtttcttggt ccatggctaa ggagccaaat ggtggcttta
    17461 ttattcaaat taggattaga aaggatgctt gatgtataag gtactattag ttgacgatga
    17521 atacatgatt ttacaagggc tcaccatgat tattgattgg caggcacttg gatttgaagt
    17581 ggttcaaaca gcaagatcag gcaaggaagc cttagcctat ttgactcagt acccagtgga
    17641 tgtcatgatt tcagatgtta ccatgcctgg aatgacagga ctggatttaa tagaagcagc
    17701 taaaacctat catcctcagc tacaaacctt gattttatct ggttatcaag aattctccta
    17761 tgtgcaaaag gctatggaat tagaaaccaa ggggtatttg ctaaaacctg tggataaggc
    17821 agaattacag gctaaaatga aacaatttaa agactgcttg gatgcccagc aggcagaatc
    17881 aatccggcaa gaagcctatc atgacagcct tttgaccctt tggcttacag atgaattgag
    17941 tgagaaagaa tttcagcagc taagtcaagg cttgccagcg gctgctttaa ctggttttac
    18001 agtgctatat ttggactgtc aagagtggca agcagccatc gatgcttatt ttgaacgaga
    18061 aggtcagcct ttttacctca aaaaagaaga agataagaag gtttatctgg ctgtcctact
    18121 tggtaaaact gatcaagcca aggcatttac ctatgatcta caaaagcgat tctcaagaaa
    18181 catgcatcaa cttattttgg gagaaacagt tgatgactgg gaaaatgttt atgaaagcta
    18241 caatcaggtg cttaagagcc tcttttacaa ttgtgaagtg tcaccaatta gagcaaaaga
    18301 taagtccaaa attgaactac cagagtcccg tttgcagttc tttgctttta acaaggcgtt
    18361 gatgattggg gacgaaccta caatcttgtc taaattagaa gctatttttg acgagatgaa
    18421 gaccttgaac ttttcaccag aggatgtgaa gcatgtttcg ttcttgttgt tttctgacat
    18481 ttaccgtcaa tttcccattt tagataaaat gacttatctg tccatggtaa aaactattca
    18541 tgacagccaa tccattgatc gcattttgag ggaactaaaa aaagtgttgg atgtcaccaa
    18601 tcagaacaat tcccctgaaa aacgctattc agatttagtt agtgaaacca ttgattgcat
    18661 tcgcaaggaa taccatcaag aattaaccct taaagctatc gctgataggt tacatgtcaa
    18721 tggggtttat ttagggcaat gctttaaaaa tgaaaccgag cgtagtttta cccagtactt
    18781 aaatcatgtt aggattcaaa aagcgcaaca gttattgctt tacaccaacc aatccatcaa
    18841 tgaaattgct tatgaaacag gttacaatac caatcattat tttatcaaga tgtttaaaaa
    18901 gttgaatggt ttatcgccaa aagagttcag agatcgttac aaggacaatt accaagccat
    18961 caagggcgat cagtaacgtt aaacaggaga ttgatatgac accagatagc aaggagcaga
    19021 cagtgactgg ctaccaatac cactatattg atcaagaagg aagaaagcag ccctttaatc
    19081 aaggctggcg ttttcttatg gcagatgtgg cgtgtgctca ggacccttct tttgatgatt
    19141 cgaactggca ggtgattcat ttaccacatg atttcagtct taagcagcct tacacaagaa
    19201 atgctgaagc tgaaagtgct tataaattag gtggtgtcgg ttggtatcgg cattatctcg
    19261 tgttagatga ggttttagct ggttgtcatg ttgccattac ttttgagggt tcctatatgg
    19321 aaacggaaat ttatgttaat gggcaattca ttggcaaaca cttaaatggc tatcaggaat
    19381 ttacctatga tatctctgat gtagtgactt ttggagctga aaatcttctg gctgtccggg
    19441 ttgaaaacaa ggttcccagt tctcgttggt attcaggcag tggtctttat cgtgaagtga
    19501 gtttgtctgt tcttccccag cttcattttg tggcagacca ggtagcaatg accttggctg
    19561 atactgctgt gcaggagaaa gggcaacaga aagttgattt acgcttttcc cttaatcagt
    19621 ctattcagac ttgccactac caattgagcc tctgtttatg ggaacaatca cattgctcaa
    19681 aagacaaaaa actcctttat caagaaacag aagtgcctct agctgatttg gctttccaaa
    19741 gacagtatgg tcttacactg tctttggaag aactccaatt gtggtcacca gataatcctc
    19801 acctctacga tttagaactc actctttact atcaaggtca ggtcattgat tgcttttgcc
    19861 ttgaaactgg ttttcgtcag ctgactttta tggccaatca ggggcttttt gtgaatggca
    19921 gagctgtcaa attaaaggga gtttgtttgc atcatgacca aggaggccta ggagcttgtg
    19981 cttacgaaga tgccctagcc agacaattag tcctgttgaa agacatgggg gctaatacca
    20041 ttcgtagcac ccataaccca agtagtccca aacttaggca actagccaat cgtctcggct
    20101 tttttgtgat tgaagaagcc tttgacacct ggacctatgc caaaaatgga aacgttaatg
    20161 acttttcaaa ctatttccat cagactatcg gaactgaaaa tgcaaactat cttcaaagag
    20221 tgagatcacc tgaaacgagc tgggcccagt atagtattga ggccatggtc tggtctgcca
    20281 agaatgaccc gtcagtgttg atgtggtcga ttggtaatga attgatggaa ggtttttcag
    20341 ccgatgtcag tcattaccca gagctgacaa gacaaatgtg tcaatggatt gctgctattg
    20401 acacctcacg tcccattacc tttggggaca ataaattgaa agaagctgac ttttgctggc
    20461 atgaggaagt gtcgcaaatg gcaactcttc ttagtcaatt agaccatccc cagggactca
    20521 ttgggttaaa ctatgctagt ggaaaagact atgataagct tcatgaggag cactctgatt
    20581 ggctgttgta tggttctgaa acagcctcag ccattaccag cagggcttat tataaagaga
    20641 caaaaaaagt tcttgactcc ggttatcacc taacctctta tgatcatgct aaggttgatt
    20701 ggggtgcctt cgctagccag gcttggtatg ataccattac ccgtgatttt gttgctggcg
    20761 aatgcgtttg gacaggcttt gattatttag gagaacctac accctggaac aagatcgaca
    20821 gcggtgttgt aggcctttgg ccttctccta aaaatgccta ctttggcatt ttagacacgg
    20881 caggctttcc aaaagatagc tattattttt accaaagtca gtgggcgcag gggcagacca
    20941 ccttgcacct gttaccagtc tggcagaagg accagctttg tttcgatgag cagggattgg
    21001 tggaggttgt ggtttatagc aatgctgcca gtgttcaact gatgtttgaa gatgagcagg
    21061 gcaacgtaac agattatgga aggaaggcat ttcatactta tagcacgcca acgggacaca
    21121 cttatcagct ttaccaagga gctgacgctg ctaagaatcc tcacgagaat ctttacctga
    21181 cttggcgagt gccctatcaa aaaggtttgc taagggcagt ggcctacgac atttctggta
    21241 aaagcattcc aaaaaccagt ggacgttctc aggttagaac ctatggttca gtggcaaagc
    21301 tatcttggaa agcctttgaa gcacctattg acgctccttg ggaattgctt tatctggatt
    21361 tatcactctt ggatagccga ggtgaattgg tcagccatgc tcaagattgg ctaaaggttc
    21421 aagtagaagg accagcccat ttgttggctc ttgataatgg gaatccgact gaccacaccc
    21481 cttatcagga gcctctacgc caggcatatg ggggaaaatt attagccatt ttagccctca
    21541 ctggagaggc gggacacatt aaggtcacgg caaaagtctc gcagcaactt agcagtgttt
    21601 ttcagacaca agtagcctgc cgtgccccca aagaacactt agtgacacta cctttagaga
    21661 aatatcagtt taaaaagagc cgactgacaa gtcaacaaga aattggtcaa tcttgtctaa
    21721 agaccacaca attcctttct gttttggaga acaaagacac ccagcaaaaa gcaggtaagg
    21781 ttagcacacc tcacattctt atcaccagtt accagcacta tttatctctg cccttgcagg
    21841 tttgttctca ggcatttgat tttaaaaaag gcgacgatat cttcttgcct cagtggctcc
    21901 aagtcaaaga tgcccaaggt tcttttgtga aaaaagcttt tccaatgaca tggtacctaa
    21961 aagagaaaat ctttgccaag cctattcttg tcacgggcta tgtttcttgt tttgggcaat
    22021 caataccggt tgaggcaagg cttaacctag cacaagctag caaaagacgg caccaggatc
    22081 tcagtttagt cgccaagcaa tccctggcca caagtcaaaa aaggctaact tattgctacc
    22141 aatatgatac ggtgcaagcc ataggagcca ttagcttgca aaatcacaca gacacatctc
    22201 ttagtgtttc tgtggcaatc agttatggta gtcagagctc tattaagcaa gaaaccttga
    22261 tgctagctga tagccacttg agccagttag aagaggttat tttcttcgat gatattttga
    22321 ctgttttgta tctagtagtg acccttgaag tcattgaagg acctgaccta actgaagaca
    22381 gtattcaaat tggcctttgg gatttagcat gaagcataag taaaagaaga cttctttggc
    22441 accaggtctg atgaattttg taaaataagg tataataaaa aacaaatagg cgtcctttat
    22501 tacgtgaaag gcgcctttac aaggttgaaa tggaaagtga gatttctaaa tgtcagaacg
    22561 tttatcaggc cacaccctgc tggtctcttt gcttgctact cctattcgtc atagtttgtc
    22621 tcccaaaatg cacaatgaag cctatgctaa actggggctg gattatgcct accttgcttt
    22681 tgaagtaggg acagagcaat tagcagatgc cgtgcaaggg attcgtgcct taggaattcg
    22741 tggctccaat gtgtctatgc caaataaaga agctatttta cccttattgg atgacttgtc
    22801 cccagctgct gagttggtcg gtgcagtcaa tacggttgtc aataaggatg gtaaaggaca
    22861 tctcgtgggg catattacag acggcatagg agcgctcaga gccttggctg acgaaggagt
    22921 gtcggtaaag aacaaaatca ttaccctagc aggagttggt ggggctggga aagctatcgc
    22981 tgtccagctc gcttttgacg gcgccaaaga gattcgtttg tttaaccgac aagctacacg
    23041 tctaagtagc gttcaaaagc ttgtgacaaa actgaaccag ctaacaagga ctaaagtaac
    23101 ccttcaagac ttggaagatc agactgcctt taaggaggcc atcagggaat ctcatctatt
    23161 tattgatgct acaagtgtag gaatgaagcc tttagaaaat ctaagtttga ttaccgaccc
    23221 cgagctcatt cgcccagact tggtggtctt tgacatcgtg tacagcccag cagaaaccaa
    23281 gttactagcc tttgcccgtc aacatggcgc ccaaaaagtc attaatgggc tagggatggt
    23341 gctttatcaa ggagcagaag cttttaaatt aatcactggt caagacatgc cagttgacgc
    23401 tattaaacct cttttaggtg atgagtaact caaccttttt taaagaactt tttaagtaag
    23461 cttaattatg atatattaat aagagatgga gcagtcatca tgggctgcta aaattctttt
    23521 tgcgagagct gtttttaaga gtaaaaagct tatttgaaga gccttatccc taacgtgata
    23581 caagtcatag ttcctttgag gtctgccaga taacctaccg tttttttaga aaagaagctc
    23641 ttataaaagg aaaagttcaa tgaataaagg agaatagcaa tggtaacaaa ggaagttggg
    23701 catgactttt tagcacgtct aggaaaaaaa cgcttaagac ctggcggtgt tgaggcgaca
    23761 aactggttga ttgaacaagg acaattctct caggattgtc acgttttaga ggttgcctgc
    23821 aatcgctgca cgacagcaat agagttagcc aaaaccttcg gttgtcagat tgatgccgtt
    23881 gacttaaacc ccaaggttgt caaagaagca caagaaaggg ttactgttga ggggttaacc
    23941 cgtcacatta cagtatccca agccaatgct ttatcactac catttccaga taaccatttt
    24001 gatattgtga ttaatgaagc catgttaacc atgttgaacg ccaaagccaa ggagaaggca
    24061 ctcaatgaat attggcgtgt gttaaaacca ggcgggcgac ttttgacaca tgacgtagct
    24121 tatgaagatc caaaaacaag acaagtactt gaagagttac gtcaaacgat tcatgtcaat
    24181 gttgctccct tgtcactaac aggttggcaa gccttatatc aagaacacgg tttttctcaa
    24241 gtgacttatc accatgggaa aatgtctttg atgacacctc gtggaatgat caaggatgaa
    24301 ggtcttatga acaccctgaa cattatgaga cgaggcctta agaaagagaa cagacaacag
    24361 tttttaaaga tgcgtcgttt ttttaccaga acaggcaaaa atctctgcta tattgctgta
    24421 aaaagtgtaa aataaacctt agaccaacct gtccatacaa ctaaacagac catagaggag
    24481 ttcgaagatg tcttatatca ataatattga gcacgcgaag gttcttgatt tgacgcaaga
    24541 agtaatgatt gagcaagatc aaatgttaag tagaacacta gtccaaaggc aagacctagg
    24601 aataacagtt ttttccttag ataagggcca agaaattggg cgacactcat caccaggaga
    24661 tgccatggtt actatcttaa gtggcttagc agaaatcacc attgatcaag agacctaccg
    24721 ggttgctgaa ggtcaaacca ttgtgatgcc agctgctatt cctcatgcct tatatgcagt
    24781 agaagccttt caaatgcttt tagtagtagt aaaaccggag gcttaacatg attgaaaatc
    24841 tctctcaact ggtgcaatat catgatcatc aggtggctag ccgatccttg agccgtctac
    24901 tgggattatc tcaatctatt gttttatatg ccatggccca aggagaaagc attagtcaag
    24961 agactagtcc aagagataaa gtaatacttg ttttagaagg tcagctcatt ttcgatttag
    25021 aggatcaaaa acaggtccta actcaagaaa gtctcatagc catacctgcc caaaaagccc
    25081 atcatttaga agctaaaacc gattgtaaac tattacaagt gcttttagat taagggctta
    25141 ttgtcaactg tattgggtgg cctatggaaa agcaaaagag aagatgacgc tcatcttctc
    25201 tttttacgta ttcaaagcga tgacaatacg aattttaaag cttggccttc tcatctccag
    25261 attaatccct ttagcttagt ttattttaga attcactaaa aaaaccttaa tgagtttcct
    25321 atctccttat aggtttctca ttaaggcttt ttagacgaaa taattgaacg acaggcaaaa
    25381 gaaagatctt tttacgagtg ataccgttct cctatagttc cataaatcct ctttttgatg
    25441 ttgctgttag ttattcttag ctaaaagtta tattgttatc aagacttgtc ttgtagtaac
    25501 atgttttagg agttccagga taccgttatt ctcttagcaa gcttaactag ctagagtgga
    25561 gtcactattt ataataaact gtcgagcttc tcgacaaatt ggtgatcgtc tttgttaatg
    25621 gctagggtgg taaaaccggt ttctgtgggt aatataaaga taatatgttg ctcagaattc
    25681 ttcttatctg ttttaaagac gtcaaagagg gatgtcgcag agacatcagc tctttcaagt
    25741 gttgttggta actggtaacg ttcaaaaact tgaaggaggc tatccaagtc ctcttttggc
    25801 aagagtcctt tggaaacagc cagtttagca tcaaccacca taccgatagc aatggcttca
    25861 ccgtgatggt aaaaatcatg gtgacagagc gattcaatcg cgtggcccaa ggtatgccca
    25921 aaatttaaag acatgcgcaa accattttca aattcatctt gttcaacaat ccgtttttta
    25981 ttggaaatgg attggtaaat cagacgttca agtccctgct tatcggtttc tttttgaaaa
    26041 tcacgtaact gctgaaaaag cggtctatca tgaatgaagc cgattttgag catctcactg
    26101 atgccgcagg agaattcccg ttgcggtaag gtttccaaaa aggtggttga aataatgaca
    26161 gcttctggag ggtaaatgct gccaatcata ttggttagac ttttaaaatg gactccaact
    26221 ttgcccccga tactgctatc cacctgacta agtaaagtcg ttgggatttg aatgtaggga
    26281 ataccacggt aataggttgc agcgacaaaa ccacccagat ccccaacaac accaccacca
    26341 atagtaacaa tcatatcttt tttggaaaaa ttttcagcga tgagctggtc gtagatagca
    26401 ctgacacgtt tcaaggattt ggattgacct cccggggggc agacatggac aaaagcgttg
    26461 tattgctgag caaattcttc aaagagagtc tgatacaagt ggtagaccgt ttgatcggtg
    26521 ataaacaaga gtttacgctg cttgcgctca ccaaggcaat ctgctaaggt ttttaaaaca
    26581 tggtcggtaa acaggatatc ataatccttg acccgagaat ggacatgaag tgtttgtggc
    26641 ataagcactc ctttcatcag tactaggtaa cagagataac gcagtgatat ttagagctta
    26701 atcttgccaa agttcctgcc ccaactgttc taattgctta taatcaattt gctgggcagc
    26761 atctgacagc gcatggtcgg gatcaggatg aacctccatc atgatgccat tggcaccgac
    26821 agccttcgct atcttagcag caggtaagag taaatctcgt ctgcctgtcg aatgagacac
    26881 gtccacaata atcggtaaat ctgtcttttg ttgaataata gggactgcca taatatcaag
    26941 catatttcta gtctccacat cgtagccacg aacgccacgt tcgcataaga taatgttaga
    27001 ttttccagtg tcttgtaagt aggaaagggc gcccaaatat tcctcgatag tagccatcaa
    27061 gcctcgttta aacaaaatag gcttatcgat gtgtgacaag atttttaaaa attcaaaatt
    27121 ttgcatgttg cgggcaccaa cctgaatgac atcgagatag tcataggctt cttctaattg
    27181 gcgctcggac atgatttcac tgaccgatag taagccaaat tcctgacaaa cctcatgcaa
    27241 gtaacgaatt ccttgcaggc caaggccttg aaaagaagca gcagatgttc ttggtttata
    27301 ggcgcctcct ctaaaatagt tgtaaccaag tttttttgca cttgaagctg ctaatctaat
    27361 gtgatcataa gattcaatgg aacaaggtcc aacaataaaa ttattcttag aacaagtctt
    27421 attttgaaag tcagacataa tgtcaatcat tcgtttgatt cctttactaa ttctgtatgg
    27481 cgtgctctat gggataacgt cttatttagg aatacaaaat ctcgttgtta tccatgaagc
    27541 acccttagcc tgtgaaatga taccataaac aagatgaaat agcaagtaaa ccctagattg
    27601 tctatgttct tagtcatttt tattaaaaaa ggcttgctag ctataaaaaa atactgcccc
    27661 aaatcttatt aggacagtat tttttataga tgccaccacc gagaatcgaa ctcggaacga
    27721 agcattacca ttgctttatt ataccattta actacagcgg ctaccttttt ataatactat
    27781 aagttattaa ttgatgcaag cttttcttaa aaatatttcc ttaattgttt atcatcaaaa
    27841 tgagagtacc agttattaat aacatggcac aagaggcctg ccttatggta taatggtaaa
    27901 gatgatttca gaaagaggaa gctaagcatg tcttatgaaa aagaattttt aaaagatttt
    27961 gaagactggg ttaaaactca gattcaggtc aatcagctag ccatggcaac cagtcaagaa
    28021 gttgcccaag aagatggcga tgaaagggct aaagatgcct ttattcgtta tgaaagtaag
    28081 ttagacgcct acgaattttt actaggtaaa tttgacaatt ataaaaatgg taaagccttt
    28141 cacgatattc cagatgagtt atttggagca aggcattatt aaggacaagg agacacgaaa
    28201 tagtggcaaa acaaaaaaat cgtaaaaaag cattgaaatt agaaatgaag cgtaatggct
    28261 tgttaaaaaa agcaggtcaa gtttttgata aggcagttga aagtgttgaa acagctgtgg
    28321 ataaaaccat ttctgctggt aaaaatcttg tggaaaaagg cagccaaacg gttggaaacc
    28381 tgacagcaag caaggaaaga cttactcttg atgagtttgc agggcagcca gttgtagctg
    28441 gtatccgctc tgaccttgtg gaaaccctat atgcagaagg catccattca gcgcaagctt
    28501 ttaaggagtg gacagaaaaa gacctccttg ctcttaaggg cattggccca gcaacggtta
    28561 aaaaattggt cgaaaatggc gcaagcttta aaaaatagtc atcttctcgt tgcttaatgg
    28621 ctataaattt gttagaatat tagcaatcag aggtgcattg agttggtctg ttacagagag
    28681 cggtggtgtt gagaaatccg cacagatgtc aaggctagta gctgaggatc agaaatcaga
    28741 tatgaaaagt agcagaccca ttttatggtt tgaaacagtg gtggtaccgc ggaaatcgta
    28801 acacgaaatt cgtccctgtc taggttatct aggcagggat tatttggttt catttgaaag
    28861 aagatgatgg cttattgtta aaatgttgtt agaagtatat aatagaaaaa ctttgcccaa
    28921 agtggaaaca gaacggctgc tattacgtca acgaaaaggg gacaaggcat tgttccagaa
    28981 gcggcttcag ctctgattga aattggtttt accttacttg atttgcataa aattgagctg
    29041 ggttgctacg actacaacaa gcaaagtcag gcagtggctc gcaaattagg ctttacctta
    29101 gaagccaatg cccgtggccg taaggacgtt caaggtcgcc gctgtggcga catgcgtttt
    29161 gggttactga gaagtgagtg ggaggagcag aaacaaaaat gaaccttatc atcattggtg
    29221 ctcaagcgtc gggcaagatg accattgggc aagaagttgc caggcagaca ggcatgactt
    29281 tgtttcataa tcacgattct attgactttg ttttacggtt tatgccttgg tctcaagaga
    29341 gcactgcctt gattgaacgt atccgttttg ctttttttga aacctttgct aagacaggtc
    29401 aagacatgat ttttactatt gtaattgact tcaatgatcc aaatgatgta gcaatgcttg
    29461 aaaagattca ggctgttttt cagtcttatg accaagaggt tctctttgtc gaattaaaaa
    29521 cagatataga ggaacgtctt aaacgcaatc gcacagaaaa tcgtctcaaa cataaacctc
    29581 ttaaacgcaa catcgagtgg tcagaacaag atattcaatc cacgatggca tatgctgttt
    29641 ttaatccaga agaaccaccc aaaacgctca cgcattacca aaaaatcaat aatacccagc
    29701 tcaccgctgc tgaaacagcg cagcttatta tccaaaaaat gactcatatc aaggagaact
    29761 aagatgacag aactatcacc taaatacaat cctgccgagg ttgaggcagg tcgctatcaa
    29821 aaatggcttg atgcagatgt tttcaagcca tcaggagacc aaaaagcaaa accgtattct
    29881 atcgtgattc caccgccaaa tgtgactggt aagttgcact tgggacatgc ttgggacaca
    29941 acgcttcaag atattatcat tcgtcaaaaa cgcatgcagg gctttgatac gctttggttg
    30001 ccagggatgg atcatgcagg gattgcgaca caggctaaag tagaggagcg tctgcgcgag
    30061 caggggattt ctcgttacga ccttggtcgt gacaaattcc tagacaaggt ctgggagtgg
    30121 aaagacgaat atgccactac gattaaagag cagtggggca aaatggggct ttctgtggac
    30181 tacagccgtg agcgcttcac ccttgatgag ggcttgtcaa aagcagttcg caaggttttt
    30241 gttgacctct acaaaaaagg ctggatttac cgtggtgaat tcatcatcaa ctgggatcct
    30301 gctgccagaa ctgccctttc tgacatcgag gttatccaca aggatgttga gggagctttc
    30361 tatcacatga actatatgtt ggaagatggc tcacgcgccc ttcaagtggc aacaacgcga
    30421 ccagaaacca tgtttggaga cgttgccgtt gccgtaaatc cagaagatcc tcgttacaaa
    30481 gatttgattg gcaaaaatgt tattttacca atcgtgaata aattgattcc aatcgtgggt
    30541 gacgagcatg ccgatcctga atttgggact ggagttgtga aaatcacgcc tgcccatgat
    30601 cctaacgact tcgaggttgg tcagcgtcat aacttaccac aagtcaatgt catgaacgat
    30661 gacggtacca tgaatgagtt agctggcgac tttgcaggca tggaccgttt tgaagctcgt
    30721 caagctaccg ttgctaaact tgaagaactt ggcgccctag taaacatcga aaaacgtgtt
    30781 cactcagtag gtcactcaga acgttcaggt gcagccgttg agcctcgtct atccacccaa
    30841 tggtttgtaa aaatggatga gcttgctaaa caagctatgg acaaccaaga aacagatgac
    30901 cgagtagatt tctacccacc acgctttaat gataccttcc ttcaatggat ggaaaatgtc
    30961 catgactggg taatctcgcg tcagctttgg tgggggcacc aaattcctgc ttggtacaat
    31021 gcagaaggtg agatttacgt tggcgaagaa gcaccagaag gtgatggctg gactcaagat
    31081 gaagatgttc ttgacacttg gttctcatct gctttatggc cgttctcaac tatggggtgg
    31141 cctgacacag atgtagaaga tttcaaacgt tatttcccaa cctcaacctt agtaacaggt
    31201 tatgacatca ttttcttctg ggtgtcacgt atgattttcc aatcgcttga atttaccggg
    31261 cgtcagccat tccaaaatgt cctgattcac ggtcttattc gtgacgaaga aggacgcaaa
    31321 atgtctaaat cattggggaa cgggattgat ccgatggatg tcattgagaa atacggtgcc
    31381 gatagcttac gttggttcct ctcaaacggc tctgccccag gacaagacgt gcgtttctca
    31441 tacgaaaaaa tggacgcctc ttggaatttc attaataaaa tttggaatat ttctcgctat
    31501 atcctcatga ataacgaagg gttgaccttg gaagaagctg aaagtaatgt ggctaaggta
    31561 gctgcctcag aagctggaaa tgtgactgac cagtggattc tccacaatct caatgaaaca
    31621 atcgctaaag ttacggaaaa ctttgacaag tttgagtttg gtgtggcagg acacattctt
    31681 tacaacttta tctgggaaga atttgccaac tggtatgtgg aattgaccaa ggaagtactg
    31741 tatagcgaca acgaagctga gaaagttatc acgcgctctg tccttcttta tactttggac
    31801 aaaattttac gtctccttca cccaatcatg ccatttgtaa cagaagaaat ttacgctcaa
    31861 tatgcccaag gttctattgt gacggtggac tatcctgtgg ttcgcccagc ttttgaaaat
    31921 gaagctgcgc ataaaggtgt ggaaagtttg aaagacctga ttcgtgcagt tcgtaatgcg
    31981 cgcgctgaag ttaacgtggc accatcaaaa ccaatcacca ttttggtgaa gacagcagat
    32041 agtgagttgg aagacttctt caccagcaac gtgaactaca tcaaacgttt caccaatcct
    32101 gaaaaattag aaatcagctc agccattgct gcgccagagc tggctatgac cagcatcatc
    32161 acaggtgctg aaatctactt gccactagca gatttgctta acgtggaaga agaactcgct
    32221 cgcctagata aagaactggc taaatggcaa aaagaattgg acatggtcgg caaaaaactc
    32281 ggaaacgaac gctttttagc caacgccaaa ccagaagtcg tccaaaaaga aaaagacaaa
    32341 caagccgact accaagccaa atacgacgca acccaagaac ggattgcaga gatgaagaaa
    32401 attaaatcat agtgtttaga acgcagttga aaaactgtgt tcttttctta ctaatagaga
    32461 tattagattg aagtttaatt atgagaaaaa cgctttcttt atttaaagag ttatgttaca
    32521 atactattac aatcatcgta caaatagtgt tattttgagg ggcatatgag aatgatttta
    32581 gcacactatg actgtaaaaa agataaaaag caatctttag atgagcattt atggcatgtg
    32641 gcctgttcta gtcgacagga agcatctata attggtcaag gagatgtgct ttttttaatt
    32701 ggtctttacc acgacctggg caaagctgat cgaacctttc aagataaatt attaaataat
    32761 ccaaatcggc atgttgatca ctcttatgca ggggcaaaat acttatgttc tattattggg
    32821 cctcatctaa aaaaccgagg ggttgataaa aatgagagaa tgacattcaa cgaaatggtg
    32881 gggtatgtca tctctgctca tcatgggatg tatgatttat gctactattt tgacgatgct
    32941 gaatattatg gctttaataa gtttaaaaat cgtatcaata gagacttaga tggttatcac
    33001 tatcatgaag atattaaagg gtacgctcta aaattagaaa aaaattatgt gattatggct
    33061 acaaagattt aagggagctt attgataaag cttttgataa ttaccaacaa tccatgtctt
    33121 ccttaaactg gcaagataag agtgagtggg attattatca gtcttgtatg gtgagacttt
    33181 acttgtcact cttaaaaaac gctgatattt tggacacagt aaatgtctat ggccttaaga
    33241 taagtcctat ggataaaaca gagcgatcct ttctaaaaca ctcctattta gcggccattg
    33301 aacaaaaata tgctagcttt ggacagccaa acaatcagtt gaacactatt cggacagaaa
    33361 tcgctgagcg tgttaaagaa agaggtaaac gagattccaa ggggatttat cgcttagatt
    33421 taccgacagg agctggcaag actaatctta gtatgcgtta tgcgtttcac caattagttc
    33481 atcacgacaa atcaaggttt ttttacataa cgccctttct ttcggttctt gagcaaaatg
    33541 cttccgaaat tagaaaagtt acaggtgacc ttggcgttct agaacaccat tccaatgtgg
    33601 tgaaacaggc taatgaagat gatgatgaca aggacagttt attgtcagct tatcttagtg
    33661 atagctggga cagtcaagta gtcttgactt ctatggttca atttttccaa acacttttca
    33721 aaacaaaatc agctaatctg agacgttttt caagtttgat taatagtgtt gtgattctag
    33781 atgaagttca atccctgcct attgaagtca ccactttgtt taatttaacg atgaattttt
    33841 taaataaagt tatggataca accatcgttc tttgcacagc aacacaacct gcttatgatt
    33901 cttcagagat tgaccatcgt atctgttatg gagggaactt gggagaatta gctgaaatcg
    33961 ttgagttaac gattgaagaa aaacagattt tttcaaggac agagcttaga aaatttgatg
    34021 atagtgatca gaaagttcac ttgactgatg ttattaacct tattctaggt gaggaaaact
    34081 cagttcttgc tatttttaat acgaaaaaaa cggttcataa ctgctatact atgctaaaag
    34141 acatgactga tagaccggtc tatcagcttt cgacaaatat gtgtgcgcag catagacttg
    34201 acttgattgc taagatcaaa acggagttac aaaataatat ccctattatt tgtattagca
    34261 cgcaattaat tgaagcaggt gtagatgttg attttcatcg cgtcattcgt tcctactcag
    34321 ggattgattc tattattcag gctgctggac ggtgtaaccg agaaggcaaa cgagataaag
    34381 ggcaagtcac tcttgtcaat ctgaccaatg aagaggaaaa tatttctagg ctgacagaaa
    34441 taaaagctaa aaaagaagcc acagaatcta ttcttcataa gattgggtct ccaattgata
    34501 tcttaacttt aaaccgtgac ttttttgagt attattatgc caataatcag ggactgatgg
    34561 attatccttt ggaagacaac ctatcaatct acgactattt aagccttaat atttatcaga
    34621 cggcaaataa aaagttcaaa ggtaagttaa aacaagcttt taaaacagca ggagccaaaa
    34681 tgaacctcat caataatgat atgataggaa ttctcgtacc ttatggcgaa gctgagaaaa
    34741 aattggctta tttagaagaa ttgggtatgt ctcacttttt atcagcagaa gattatcaaa
    34801 cgataaaatc attactaaaa gagttacaac cttttacggt taatgtccgc gagaacgatc
    34861 ctctctttga gacaacaaaa tcttatctaa atggtcagat tctggttttg acgtcggagt
    34921 attatgacac ggaaagagga gttaaatacg attcagctag cttttacttc taact
//
LOCUS       DAUCWO010000009        34666 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUCWO010000009 DAUCWO010000000
VERSION     DAUCWO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.009"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(14..658)
                     /locus_tag="VGY20_000433"
                     /pseudo
     CDS             complement(14..658)
                     /locus_tag="VGY20_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            943..1170
                     /locus_tag="VGY20_000434"
     CDS             943..1170
                     /locus_tag="VGY20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ4273317.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGSPI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1183..1383
                     /locus_tag="VGY20_000435"
     CDS             1183..1383
                     /locus_tag="VGY20_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ4273318.1"
                     /translation="MTTMKELTINDMASISGGNTPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            1922..2203
                     /locus_tag="VGY20_000436"
     CDS             1922..2203
                     /locus_tag="VGY20_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273319.1"
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRH
                     PYPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEIKINSTEL"
     gene            2397..2800
                     /locus_tag="VGY20_000437"
                     /pseudo
     CDS             2397..2800
                     /locus_tag="VGY20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3051..4121
                     /locus_tag="VGY20_000438"
     CDS             3051..4121
                     /locus_tag="VGY20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273320.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSGTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSDKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYIRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIK
                     VSINPKGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
     gene            4214..4612
                     /locus_tag="VGY20_000439"
     CDS             4214..4612
                     /locus_tag="VGY20_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ4273321.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(4960..5229)
                     /gene="stcA"
                     /locus_tag="VGY20_000440"
     CDS             complement(4960..5229)
                     /gene="stcA"
                     /locus_tag="VGY20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ4273322.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(6143..6211)
                     /gene="shp2"
                     /locus_tag="VGY20_000441"
     CDS             complement(6143..6211)
                     /gene="shp2"
                     /locus_tag="VGY20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ4273323.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            6300..7166
                     /gene="rgg2"
                     /locus_tag="VGY20_000442"
     CDS             6300..7166
                     /gene="rgg2"
                     /locus_tag="VGY20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ4273324.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            7338..8165
                     /gene="mutM"
                     /locus_tag="VGY20_000443"
     CDS             7338..8165
                     /gene="mutM"
                     /locus_tag="VGY20_000443"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ4273325.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKEIFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIKKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKSCPRCGQDIVKLKVGGRGTHICPKCQKKRP"
     gene            8162..8755
                     /locus_tag="VGY20_000444"
     CDS             8162..8755
                     /locus_tag="VGY20_000444"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ4273326.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKTDGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            8945..10447
                     /locus_tag="VGY20_000445"
     CDS             8945..10447
                     /locus_tag="VGY20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ4273327.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISELGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNVTKQSVGAIQRSLLTLEQEGAEQFFGEPALEVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSDI
                     ESQQLVQSSPFASKYSQSIDRDSAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKASRRSVGRPRKTVVEKATDAFISTTVKTIGRELVRGLLGSLRKR"
     gene            10569..11762
                     /locus_tag="VGY20_000446"
     CDS             10569..11762
                     /locus_tag="VGY20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ4273328.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVTPILSLYIRHLGQIHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVVAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            11759..11905
                     /gene="rpmG"
                     /locus_tag="VGY20_000447"
     CDS             11759..11905
                     /gene="rpmG"
                     /locus_tag="VGY20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4273329.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            11951..12187
                     /gene="secG"
                     /locus_tag="VGY20_000448"
     CDS             11951..12187
                     /gene="secG"
                     /locus_tag="VGY20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ4273330.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            12281..14614
                     /gene="rnr"
                     /locus_tag="VGY20_000449"
     CDS             12281..14614
                     /gene="rnr"
                     /locus_tag="VGY20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ4273331.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSSLDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINPQGYVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILETMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            14617..15084
                     /gene="smpB"
                     /locus_tag="VGY20_000450"
     CDS             14617..15084
                     /gene="smpB"
                     /locus_tag="VGY20_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ4273332.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLENELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            15090..15809
                     /locus_tag="VGY20_000451"
     CDS             15090..15809
                     /locus_tag="VGY20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ4273333.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNSHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(15926..16573)
                     /gene="pcp"
                     /locus_tag="VGY20_000452"
     CDS             complement(15926..16573)
                     /gene="pcp"
                     /locus_tag="VGY20_000452"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ4273334.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(16623..17549)
                     /locus_tag="VGY20_000453"
     CDS             complement(16623..17549)
                     /locus_tag="VGY20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEQ4273335.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEEIAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            complement(17549..18232)
                     /locus_tag="VGY20_000454"
     CDS             complement(17549..18232)
                     /locus_tag="VGY20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ4273336.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQSLTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            complement(18441..19367)
                     /locus_tag="VGY20_000455"
     CDS             complement(18441..19367)
                     /locus_tag="VGY20_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4273337.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELATRFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            complement(19512..19889)
                     /locus_tag="VGY20_000456"
     CDS             complement(19512..19889)
                     /locus_tag="VGY20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4273338.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     TLEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            complement(19900..20565)
                     /locus_tag="VGY20_000457"
     CDS             complement(19900..20565)
                     /locus_tag="VGY20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ4273339.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            complement(20614..21699)
                     /locus_tag="VGY20_000458"
     CDS             complement(20614..21699)
                     /locus_tag="VGY20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ4273340.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLIKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLVAIDFIKPGVTAAQVDAVARQ
                     VIEKSGYGEYFNHRLGHGIGMDVHEFPSIMASNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            21873..22874
                     /gene="ccpA"
                     /locus_tag="VGY20_000459"
     CDS             21873..22874
                     /gene="ccpA"
                     /locus_tag="VGY20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ4273341.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            23004..24002
                     /locus_tag="VGY20_000460"
     CDS             23004..24002
                     /locus_tag="VGY20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ4273342.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            24004..25338
                     /locus_tag="VGY20_000461"
     CDS             24004..25338
                     /locus_tag="VGY20_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ4273343.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHFAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLR
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            25760..27703
                     /gene="thrS"
                     /locus_tag="VGY20_000462"
     CDS             25760..27703
                     /gene="thrS"
                     /locus_tag="VGY20_000462"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ4273344.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            27844..28836
                     /locus_tag="VGY20_000463"
     CDS             27844..28836
                     /locus_tag="VGY20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4273345.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQTFGKMKSLSFELEAGQEEVVSQFLGLPDIT
                     VERHELTLEIQYDSSRYQTADIIQKTIADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            28838..29656
                     /locus_tag="VGY20_000464"
     CDS             28838..29656
                     /locus_tag="VGY20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ4273346.1"
                     /translation="MWSFWKRYSPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLVVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKMVSIVLSFLPL
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            29658..30443
                     /locus_tag="VGY20_000465"
     CDS             29658..30443
                     /locus_tag="VGY20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273347.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            30644..30793
                     /locus_tag="VGY20_000466"
     CDS             30644..30793
                     /locus_tag="VGY20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ4273348.1"
                     /translation="MQLLDNIYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            31188..32336
                     /locus_tag="VGY20_000467"
     CDS             31188..32336
                     /locus_tag="VGY20_000467"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ4273349.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGHYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            32293..33540
                     /locus_tag="VGY20_000468"
     CDS             32293..33540
                     /locus_tag="VGY20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ4273350.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQEKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            33596..34630
                     /locus_tag="VGY20_000469"
     CDS             33596..34630
                     /locus_tag="VGY20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ4273351.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYIISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRTPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
BASE COUNT        10574 a         6137 c         7397 g        10558 t
ORIGIN      
        1 tcttgtaagt attttagtct tcgaaaatac ttacaagaca tcgttctaag gcttcttcat
       61 ttggaaaaac caccctcctt cttgctttgt cgcttgattt ctttattgag tgattcaatc
      121 aagtttgtag agtaaatact agaccaaatg taatgtggaa attgatagaa agttaggaga
      181 ttttccgttg tttcaagtga tgtcataacc tttttgtaac gaggcttcca ctcagaaaca
      241 agtggtgacc atacatgcgc tcaataatgt cactgatttc gcgtgtcgta acgccagtct
      301 gatagagttt gatcaccatc tcttccaagt ggtcatctcg tctagcataa gatggtaata
      361 aaactggtga aaactcgccg tttcgatctc ttgggatgat taaattgact aagccatact
      421 ttgtttcaaa ctgacgagaa taagtcccat tacggctatt acctgtgttg taaccttctt
      481 tttcataagg ttcataacca agaaaagctg atagttcaac ttgcaagagg tcattcatag
      541 ctatttctaa agaggagcga aagaattcat caatatcttg tttttgtgct aggaagttaa
      601 gtaattctgt ggtaaactga gtcatgggaa taaatctctt tctagtgaag tgtcgcaact
      661 ctactataca ggatttattc ctttttgtgt gtttacacaa gatattttac actaccatca
      721 aaaatcttaa aaggtattta taaaagcatg aatggtatca tttaaacttc ttaattaatt
      781 aactattgtc acagcgtaag tgtagagagg ataaaaagac atttcatgat gaaaaacagc
      841 cttttaagat tttgaaacca tgatactgtt ctttatgata ttctgtacta gtcgaggttg
      901 caacgagaca aatttaagaa ttatgaaaag aagttattcg atatggagat aaagaaactg
      961 gaaacatttc accaaatgac tatagaaaag cttgccaagg ttgagggtgg taagaataat
     1021 tggcaggcaa atgtcagtgg ggttatcgct gcaggtagtg ctggatctcc tataggtttt
     1081 ccagtttgtg gtgtagcctg tggttatatt ggagctaaaa cagctattac tctttgggct
     1141 ggtgtaacag gagctacagg cggattctag aggaaagaaa ggatgacaac aatgaaggaa
     1201 ttaacaatta atgacatggc tagtataagt ggaggaaaca ctccaggaga tgctgtaatt
     1261 ggtggtcttg gaggcttagc ttctggattg aaattttgta aactgccaca tccagtattg
     1321 acaggtggat gtgttgtagg atttacagtt ggtggagcat atttgggata tacagcaaat
     1381 taaatgatct atacgatttt tattattatg aatgcgtcta taatgttttt aatcattgtt
     1441 gatgccattc ataaaaaaat gttgataaat ctataccata tttagtgttt ttaattttca
     1501 atcaattatc cctttcagaa aaatcggtaa acatgttatc tatcgtagct ttggtagcgt
     1561 cattatttgt ttttatcgat aaaagaaaat agtgattatg ccaataaatt tagatatcac
     1621 tcttagcttt ttaaaataca aaagagtgaa attaaaatga aaaagacttc aaaaatatgg
     1681 aagtgctaaa aaaatggtct ggctatcata aatggaggcg gaaaagacgg agcaaatatc
     1741 tttttgacag ggatggcagg tgctgcgcag ggagtgacag tttgtgctca gacaggcgta
     1801 tttatccctt ggcaaggtta tattttgtgt ggagcagctg gtgctgctac aaatattatc
     1861 tggcctcact aaatatataa atgactatct atcactgatg tataagtagt agaggagtgg
     1921 tatgaaaatt ttagatatta tttttaacct atctgataga aagatgatat ttctagaaaa
     1981 tgttgaaaag ctgggaggtt acattgtccc ttttaccctt atcatcttga gtgtagccta
     2041 caactttagg cacccttatc ctatttgggc tgctgtacta ggaggtatat cccttattgt
     2101 gatctttatt tattttatac ttattgttta tctatatttg cgttatagat acttggtgtg
     2161 gaaaaaaata ccagaaatta agattaattc tactgagcta taatgcaatc acatttaagg
     2221 ggaaaatgga tgacttatac aatatcagta cttatgatat ttaactttag agactaaaga
     2281 gttaaaagca aaatgcgtat taaaaattgg ttaaaaaagt tataactgtc tctgataaca
     2341 ggaataacaa tttgttcggt tatatttgct accgatagag tagaggaggt gaagatatgc
     2401 gtaatatatt aagattttta ggtattgtta ttatttctgt gcagtgattc tatttagtat
     2461 aggtagtttc tacgatttac cattaatgaa aaatatacaa cttatctgtt ggagttttat
     2521 ccttgattta ttcgtttttg tctacaaaca aagacaaacg acagaagtgc tcacatggta
     2581 tcaagttgtt aagcaatttt ggttatttat taagtgtact attcttattc ctaccttaat
     2641 tgcttttatt ataataatag gatgtttgac gagtatatca gaggtatcaa tttactttta
     2701 tttacacctt attgttgttt gctatacaat aggaatgatt ttatcattag gtcgtcttat
     2761 ttctcctaat cactctatat ttaataaact taaaaagtga agtgaatcat atttaaagtt
     2821 tgtttttaaa taatgggact agtttagtgt aaccgattca attaaagaat agttcgtgac
     2881 aagaagatac ctttgaagtt tcagaaataa tctgtcttct atcctgctat actcatcttt
     2941 gccaaaactc gtcattttat atgatttata tagaattacg agttttttgt tagactaata
     3001 tgagaaaata tagtatgacg aattcctaaa taaaaaataa tgaaaggaac atgatgatgt
     3061 taagagatgt taaagttaaa acgagcagtt tattagttgg ttgtgcggct actttactag
     3121 tcagtttgag tggtacagtt gcggcagatt cagtccattc gtctgataga cgttttggag
     3181 ttggctataa ggaagaaaca acctctcagt tttcttcgga tcagccctcc caggtggtat
     3241 tgtatcgagg tgaggctaac acggttaggt ttacctacac caatcagatg tatctgatga
     3301 aagatattcg cattgctttg gatggttctg ataagtcttt gaccgctcag actgttcctg
     3361 gtatgggtca tgtttatgag ggctttcaaa cttctgctag agggattttt acgatgtcag
     3421 gagttcctga aagcactgtt cccgttgcta accctaatgt acaaaccaaa tatataaggt
     3481 atttcaaagt cattgatgat atgcataaca caatgtataa aggaactgtt tttcttgttc
     3541 aaccgcaagc ttggaaatac accatgaaat ctgttgatca gttaccagta gatgacttga
     3601 accatattgg cgttgctggt attgaacgaa tgacaactct cattaaaaat gcgggtgccc
     3661 ttttaaccac aggaggtagt ggagccttac cggacaatat taaagtatct attaatccaa
     3721 agggggggct ggccacgatt acttatgggg ctggctctac ggatattatt cctccagcag
     3781 ttttatggaa aaaaggctcc gtaaaagagc ctactgaagc cgatcaatct gtcggaacac
     3841 cgactcctgg tattcctggt aaattcaaac gagaccagag ccttaacgag catgaagcta
     3901 tggtaaatgt cgaaccactg tctcatgtag taaaagacaa tataaaggtc atagatgaaa
     3961 aatcaacagg gcggtttgag ccttttagac ctaatgaaga tgagaaggag aagcctgcca
     4021 gcgatgttaa ggtaagacca gcagaagttg gtagctggct agaaccagcg acagctcttc
     4081 ctagtgttga aatgagcgct gaggacaggt taaaaagtta gttaataatt tgccggttgc
     4141 tttagacagg caaacaggaa agggcttgga ggtttcttct gaagtagctg tgagcgcctc
     4201 acagccggaa gaattgcatc gattgcctaa ccgtttgata cctgaggaag acaataataa
     4261 actatcacga gtagccaagg aagtcgttac tgagccaagg ctaaataacc cctttttgtt
     4321 acccaagaag gttctttcgt tagaagatga tgctgctatc cttaatcatt tggaggaaga
     4381 ccgtaatgtc aataagttgt tcctcgagcc agttgcggct gaagccaaga gagaaattaa
     4441 ttttcatcca agtgaccagc aggtgactgc taaagttaag gagtcatctg attttcatca
     4501 gaaacagcac caaacaggga ttacttatgg agatgatact ctggcagatc tggcgcatca
     4561 tttgcttcat tcagatggtg ttattcctag tctaaatgat aagagtgctt aagaattatg
     4621 tgctagtaag tctcgctaag aagaggagtg ttacaagata aataaagaag aggtcaggga
     4681 actaccggcc tctttttgtc cgaaatagcg agaatgactc tagcttacct gtatgtaggt
     4741 ccgtgctaca catcttgtta atgataggga gctgggatca gaaaataatt ggtatatcgt
     4801 tttctcctaa ctttttaaga aagtgcgggc aaagtgggct gttagggtga tggggttgct
     4861 ctttcatgat cagtttttta gcttgaagaa agcgtggtga tgagattagc ttaaaaaaga
     4921 acactaccga aatggtagtg tccactaatt ttaggagagc taagattggt catctaacag
     4981 gagtctaact gtcatgctaa taacagattt gatagcgctg tccccacttg gaagaaatag
     5041 agccttattt ttattttttt tgtgtttaag ggcttctttt ttgtgagggt tttgttttga
     5101 gtggcctatg gtcatcgcag tccttttcct ttcaatagtg tgtttaggaa ttggtggttt
     5161 agggctgacg gcaagaataa aacacagtat gctaatagct aagataagat aaaactgttt
     5221 tttggtcatt gtttaggcct ttctagttga aaaatgtagt tgttgttagt ttagcctttt
     5281 tagtagctgg tatttaaaat agcattagcc aagtggttgt actggctatc taaacggtac
     5341 tcagtattcc tcacgtcttt tccagcaagg aaataccttg tggtgtatgc tgaatcattc
     5401 caggtagcat caacgtagga attaacacca tgaatggtaa cgatgtttca agcatggtgc
     5461 tcattcaatc cttttttatc acataccaag ctgataagcc tgcagcttct gccatatctt
     5521 taaacacgac ggcataagct tgacaaacac cacacttgcc acgataaaga gctttaggag
     5581 tatgattgtt gatgctgctt acggttttgc gattggtgct aagttatttt gtggcgtaag
     5641 tgtattcgtt gattaaatgg ttataaataa ccttagcctt gtcgtagtca ctggtgatcg
     5701 gtttatctgt aatatttttt tgacaaaggt tttatattcc ttatgaaggg ctaccaaatt
     5761 gtctttaaga gtataggtaa ctgtaatatc actgttaacc atgatacgtt ggctttgtct
     5821 accaagtgta taagtactgg tacgactttg ggaagtaaat gaagtaaggg ttccataata
     5881 atcacctcat tcgtcgagag tggcaccagg tcgttgctgg ttaatggagc tgatagtttg
     5941 atagtaagtg ttttgttcag acacatcata acggtatgcc cctgctgttt catcagcttt
     6001 gaccttgctg tcaatggcag caaacgcact taagagtaaa gctgccgcta ggtcgctaac
     6061 actcttttta atatttatta gttttcctgt tacaaactaa atataagggt ttcccaaaag
     6121 tggattgttt ttaagcgaaa ggttatccac caacgataat caggatatcc atgatcaatg
     6181 taaaaagcaa agctttatta acttttttca tgactgtctc ctttctgatt ttctattttg
     6241 tacttgtatt atataatgga tttagcagat tttaattttt tgttgtgaaa gtgggaaaaa
     6301 tggaaaaaga actcggaaaa accttaaggc gattacgtaa gggtaagcag gttagcattt
     6361 cttttttagc agatgaatac ctgtccaagt cacaaatttc aagatttgaa cgaggggaat
     6421 cagaaatcac ctgcagtcgc ttgcttaatc ttttggataa actgaatatt acgattgacg
     6481 aatttgtttc ggctcattcc aagacgcata cgcatttttt cacgctctta agtcaggcga
     6541 gaaaatgtta tgctgagaaa aacgtggtta aattgaccaa actgttgaag gattatgctc
     6601 ataaagatta tgaacgaacg atgattaaag ctattctttt ttctattgac tcctcaattg
     6661 cacctagtca agaagaattg actcgcctca cagattattt atttaaagtg gaacagtggg
     6721 gatattatga gatcattctt ctggggaatt gctcgcgctt catgaattac aataccctat
     6781 ttttattgac taaggaaatg gtagcatctt ttgcttattc ggaacaaaat aaaaccaata
     6841 aaatgttagt gactcagtta tccattaatt gtctcattat cagtattgac cattcttgct
     6901 ttgaacatag tcgctatttg attaacaaaa ttgatctttt gctgcgagac gagctcaatt
     6961 tttatgaaaa gactgttttc ttatatgtgc atgggtatta taaattaaag caggaagaaa
     7021 tgtctggaga agaggacatg cggcaggcct tgcagatttt taagtatctt ggagaggact
     7081 cattgtatta tagttataag gaacattacc gccaaatagt gctgggaggt aagggggatg
     7141 aggactggtc tgaggcagat ttatgagttg ctttctatca gatgtattcg taaaataaga
     7201 aataagaaag gagaaggaca gctgtgtttt ttgaaacgca gtcagtagcc tagacctgag
     7261 tctattgagt gactttttta tgctgagttt attctttgta gcagcaaagg gtctagctgt
     7321 cctttaaata atggattatg ccagaattac cagaagttga aacggtccga cgagggcttg
     7381 agactttagt gcttggccaa gaaattgtgg ctgttacgct taaagtgcca aaaatggtta
     7441 aaaccgactt agagacgttt gccctgaccc tacctggtca aatcattcag tcagttggcc
     7501 gtcggggaaa atatttatta attgatttgg ggcaattagt gttggtatca catttgcgga
     7561 tggaaggcaa atatttgctt tttccagatg aggtccctga taacaaacac tttcatgtct
     7621 tttttgaatt gaaaaatggt tcgaccttgg tttaccaaga tgtgcgtaaa tttggaacct
     7681 ttgatttgat agcaaaatct caattatcag cgttttttgc caaacgaaaa ctaggccctg
     7741 aaccaaagaa agagatcttt aagttaaaaa cctttgaggc tgctttgcta tcctcccaaa
     7801 aacccataaa accacatctg ttagaccaga ccttggttgc tggtttaggg aatatttatg
     7861 tggacgaggt actttgggct gctaaggttc atccagaaac tgcgtcttct aggttgaata
     7921 aggcggagat aaaacggctg catgatgaaa ccatacgaat cctagcttta ggaattaaaa
     7981 agggcggttc gaccgtccgt acttaccgta atgctttggg agctgatggc actatgcagg
     8041 actatttgca ggtttatggt caaactggga aatcgtgtcc tcgttgtgga caggatattg
     8101 tcaaattaaa agttggagga agggggacgc acatttgtcc aaaatgccag aaaaaaagac
     8161 catgattatt ggcatcacag gaggcattgc atcaggaaaa tcgactgtgg ttaaggtcat
     8221 tagaaaagct ggttatcagg tgattgatgc ggatcaagtg gtccatgact tgcaagaaaa
     8281 aggcggtcgc ctttacgagg ctcttagaga agcttttggg aatcagattt taaaaacaga
     8341 tggtgagtta gatcgcacaa agctgtcaga aatgcttttt tcaaacccag ataatatggc
     8401 aacatcctca gccattcaaa atcaaatcat taaggaagaa ttggcggcta agcgtgacca
     8461 cttagcacag agccaagcca tttttttcat ggatattccc cttttaatgg aattgggcta
     8521 ccaagactgg tttgatgcga tttggctggt ttatgtagat gctcagacac aattgcagcg
     8581 cttaatggct agaaatcgtt tagacaaagg aaaagcaagg cagcgaatag ccagtcagtt
     8641 accaatagag gaaaaaaagc cctatgccag tctagtgatt gataatagtg gggatattgc
     8701 agccttaata aagcaagtac aatctgcgtt gttgtcatta gccaatccga ggtagctaag
     8761 ctgagagatt cgtatcatag ctgtcaaaat caagctattt ctgtctaaaa ctagctggtc
     8821 ccttttaaat tagcgcatca agggctatat gttataataa tattaagagc gagacccttc
     8881 taaacacttt aacttatggg taaacacata tcgacaagta tgatcatcaa aaaagaggaa
     8941 gcctatgact acaatgacaa ttgcaaaagg caatcaaccg atagccttac aattagggat
     9001 gctaaatcgt cacggtttga ttgctggagc gactggaaca ggtaaaactg tgaccttaaa
     9061 agtgctagca gagcaattaa gtttagctgg tgtgccagtc tttttggcag atatcaaagg
     9121 agacctgtct aatcttacta aagcaggaga ggtgactgat aaattggctg cgcgtttagc
     9181 cacaataggg gtaagtgatt accagcctca ggcctttcct gtaagaatgt gggatgtttt
     9241 tggtcaaaac ggtcaaccct tacggacaac gatttctgag ctgggaccga tgatgttgtc
     9301 gcgtttattg aatcttaatg atacccagac aggtgtctta aacatcgtct ttaaaatcgc
     9361 cgacgagaaa ggttggcttt tgattgattt gaaagacttg caggccattc taaaagaagt
     9421 tggtgaccat gcctcagact attccagcca ttacggtaat gtgaccaaac aatctgtcgg
     9481 agctatccaa agaagtctct tgactttgga acaagaaggg gccgaacaat tttttggaga
     9541 gcctgcttta gaggtggctg atttgatgca attagatgtt gcttcgggtt atggagccat
     9601 taacattttg tcagcgacta agcttttcca atcacccaca ctttacacga ccttcttact
     9661 ttggttatta tcagagctgt acgaattatt gccagaagtt ggggatttgg ataaacctaa
     9721 gatggttttc ttttttgatg aagctcactt gctcttcaaa gatgccccta aagttttctt
     9781 ggaaaaagtt gaacagattg tgcgacttat tcgttcaaag ggagttggga ttttctttgt
     9841 gactcaaaac ccacttgatt tgccagaaac ggttttggct cagcttggaa atcgtattca
     9901 gcatgccttg cgcgcttata caccgaaaga acaaaaggcg gttcgtgtcg ctgcggatac
     9961 tttccgacaa aatcctgact tagatgtggc aagggtgatt acagaactag aagtcggcga
    10021 agctttgatt tctgttttga atgataaggg acaaccatcc atcgttgaac gtgcctatat
    10081 catgccgcct aagagtagct ttgctgtttt gtcagatata gaaagtcagc agcttgtgca
    10141 gtcctcacct tttgccagta aatatagtca gtccattgat cgtgactctg cttatgaaaa
    10201 attagctgct aaggttttag aagacaaccg actggcccaa gaagcgatag cgacagcaca
    10261 acgcgaaaaa gaagctaaag aagccatcaa ggcgcaagca gctaccaaaa aagctagtcg
    10321 ccgttctgtt gggcgtccac gaaaaacagt cgtcgaaaaa gcaacagatg cgtttattag
    10381 tacgacggtt aaaacaattg ggcgagaact ggtcagaggt ttactgggta gtttacgtaa
    10441 acgatgaagg ctatcagatg agacttagca ctattatggg atactgtttt tggcagaaat
    10501 gtgttataat aatcaaaggc ccaatggaaa gaagatgatg atctattagt gataaagcgg
    10561 taacgacagt gaactggcga caaaatctaa aagtggcttg gcttggtaac ttctttactg
    10621 gggcaagctt ttcacttgtt atgcctttta tggctctata tgtggaaaat ttaggtactc
    10681 cgacagaatt agtagaatac tatgctggtc tggcagttgc tgtgacggca cttgcttcag
    10741 ctttatttgc tcctgtttgg gggaagttgg ctgatcgtta tggccgaaaa cctatgatgc
    10801 tgcgagcaag ttttgtcatg acctttacca tgggtggttt agctattatt cctaatgtgt
    10861 tttggttact tattttgaga ttattaacag gtgtctttgc gggctatgtg ccaaatgcca
    10921 ctgccttgat tgccagccaa gccccaaaag aggagtcagg ttatgcttta ggcacattag
    10981 caacaggagt gacagcaggc gctttaattg gtcctttact agggggaatt ttggcagagc
    11041 tattaggaat tcgacaagtt ttcttattag ttggtgtgat attattttta tgttctttaa
    11101 tgacagctgt ttatgttaaa gaagagttta agccgattag gcgctttgag atgataccta
    11161 caaaagtcat tttaaaacag gtaaaaagtc cccagattat gcttggtttg tttgtgacta
    11221 gtatgattat tcaaatttca gctcaatcag tgacccccat tttatcattg tatattcgtc
    11281 atcttggtca aattcacaac ttaatgttta cttcaggcct ggtagtttcg gccatggggt
    11341 tttctagctt atttagcagt tcttacttag gaaaattagg agaccgattt ggcaatcatc
    11401 gattattgtt agccgctttg tgttatagct ttatcatgta ttttagcagt gccttagcgc
    11461 agaccagttt tcaattagga gtactacgct ttgcttatgg gtttggtgtg ggcgctttga
    11521 tgccaagtat taactcttta ctaactaagt taacaccaaa agaaggtatt tctagagtag
    11581 ttgcctataa tcaaatgttt agtaaccttg gtcaagtcat tggccctttt atcggatcaa
    11641 atgtggctgt agtgttaggc taccgttctg ttttttatgt gactagtttg attgtgtttg
    11701 ttaatttaat atggagtttg attatatttc gaaaatatat taaagttaag gatattgtgt
    11761 gagagtaaaa attaatttag aatgcagtga gtgtggcagt aacaattatc taacgagtaa
    11821 aaataaaagc agtcatccgg aaaaaataaa agttcccaaa tactgcccca aggagagaaa
    11881 agtgaccctg catgttgaaa cttgataaga attgtgttag aatggtcatg acaatagtgg
    11941 aggaaataac atgtacaact tactacttac gatattgcta gtactgtcgg tattgcttgt
    12001 gattgctatt tttatgcaac ctcaaaaaaa cccaagtagc aatgtttttg atagcagcgg
    12061 ttcagaagcc ttgtttgaac gtacaaaagc tcgtggcttt gaagcattca tgcaacgatt
    12121 cacagcagtt ctagtatttt tttggctagc gattgcactt gtgcttgcca ttttatcaag
    12181 taaataagga gtgagcttga cagagttagt ctagctcatt tttttatcag cgaaacggat
    12241 tgatatcctc caacaaacaa aacagaaata gaaattaggt atgatgaaag aaagaattat
    12301 gacttacctc aaggagcatg gcaagtccaa tgtgaacgac ttggctgcgg ccttggagat
    12361 ggctggagct aagcattttc ccagccttat taaaaccatt tccaaaatgg aaagtcagag
    12421 ccttttacgg ttttcagatg atggcagtct ggctcttaga aaagagcgtg aaaagaaaaa
    12481 agaacctact gttcagggtg tttttcgtgc caataaagca ggctttggtt tcttacatgt
    12541 ggatgagaat gaagacgata tgtttatcgg tcgtaatgat gttgggtatg ctattgatgg
    12601 tgatactgtc gaagtagtgg ttaaaaagcc agcagaccgt ctcaaaggca cagcggctga
    12661 agctaaggtc gttgctattg ttgatcgttc tttaaaaaca gctgttggaa cctttatttt
    12721 agatgatgac aaaccaaaat atgctggtta tatcaggtcc aaaaatcaaa agattcaaca
    12781 aaaaatctat atcaaaaaag agcctgttgt gcttaaaggc actgaaatca tcaaggttga
    12841 tattgataaa tacccgacac gcgggcacga ttattttgta gccagtgtgc gtgatatcgt
    12901 aggccatcaa ggagacgttg gaattgatgt cctagaagtc cttgaatcga tggatatcgt
    12961 gtcagagttc ccagcagaag ttttagctga ggccaatgct atttcagaag caccaactgc
    13021 taaagattta ataggccgtg tggatttgcg tcaagaaacc accattacta ttgatggtgc
    13081 ggacgccaaa gacttagatg atgctatcca tattaagcta ttggataacg gtaactatga
    13141 acttggggta catattgcgg atgtctcata ttatgtgaca gaaggatctt cccttgataa
    13201 ggaagctatc gcccgtggca cttctgtcta tgtcaccgat cgtgtggttc cgatgttacc
    13261 agagcgtttg tcaaatggca tctgctcttt gaaccctaac gttgatcgtt tgacccagtc
    13321 agccttaatg gaaattaacc cgcaggggta tgtggtcaat tatcaaatct gtcaatcagt
    13381 gattaagaca acttaccgaa tgacgtatag tacggtcaat gacatgattg caggagatga
    13441 agaagcgctc caagaattcg ctagcattgc tgatgatgtg accctcatgg tggctttgca
    13501 taggattttg gaaacgatgc ggtcaaaacg aggcgccttg aattttgata cgcaagaagc
    13561 taaagttatt gttaatgaca aaggaatgcc ggttgatgtg gtgcttcgtc aaaggggaat
    13621 tgcagagcgg atgattgagt cctttatgct agcagccaat gagtgtgtgg cagaacattt
    13681 tgcaaaagct aagttaccgt ttatctaccg tattcacgaa gaaccaaagg ccgaaaaact
    13741 tcaacaattt attgattacg ctagcacctt tggtattcac attcaaggaa cagccaacaa
    13801 gatttctcag gaagccttgc aagcctttat ggcaaaagtc gaaggtcaac caggagcaga
    13861 agtgcttaac atgatgctct tacgctctat gcaacaggca agatattctg aacataatca
    13921 tgggcattac ggtttggccg cagaatatta cactcacttt accagtccga ttcgtcgtta
    13981 ccctgacttg ttggttcacc gcatggttcg tgagtacagt caacctagtc aagaaaagcg
    14041 cgaccatttt actcagatca ttccagaatt agcaacatct tcttcacagt tagagcgtcg
    14101 tgctattgat gctgaacgcg tggttgaagc catgaaaaaa gctgagtata tggcagagta
    14161 tgtgggtgaa gaatttgacg gtatcgtggc cagtgtcgtt aaatttggtt tttttgtaga
    14221 gttgccaaat acgattgaag gtttggttca tattactagc ttaccggaat actatcactt
    14281 taatgaaaga acgctaagcc ttcaaggtga aaaatcaggt aaagtcttta aagttggtca
    14341 acctattcgt gtgaaattgg ttaaggctga caaggaaact ggtgacattg actttgagta
    14401 tttacctagc gactttgatg tggttgaaaa aatcaagatg tcggataaag ctagtcgacg
    14461 agatcgccgt aaatcctcaa aatcatctaa agggataaag aaaaaagaac ctaaagaagt
    14521 agccaaggca aaaaccaagg gtaaaactaa aaaagggtct aaaaaaccat tttacaaaga
    14581 acaagcaaaa aagaagagtc gtaaaaggag ttaactatgg caaaaggtga aggtcatata
    14641 ctggcgcaaa ataaaaaggc tagacatgat tatcacattg ttgaaaccgt tgaagcaggg
    14701 attgtcttaa cgggaactga gattaaaagt gttcgtgcag cccgtattca actgaaagat
    14761 ggctttgccc aaattaagaa tggggaagcg tggttggtca acgtgcatat tgcacctttt
    14821 gaacaaggta atatttggaa cgctgatcct gaacggaccc gcaaactgtt gctcaaaaaa
    14881 cgtgagatta cgcatctaga aaatgaactt aaaggatctg gcatgacctt agttcctctc
    14941 aaagtttacc tcaaggatgg ctttgctaaa gttctgattg gtctagctaa agggaaacac
    15001 gagtatgaca aacgcgaaac cattaaacgc cgtgatcagg aaagagatat taaaaaacaa
    15061 atgaaacact acaatgcaag gtaaggtgaa tgagtattat gataactaaa ggacaagttc
    15121 gtttgttacg aacttattct tatgatagca acttatacac gcagggatta gaacaactca
    15181 acaacagtca tatattactt agtgccggtc gttatggttt ctcaaaagta ggggtgtatg
    15241 accttactca agagatcttt tcagaaaaaa tagcttttcc agatacagtt tttgcagaag
    15301 ggctaacagt tgttgaggat tatttttggt tactaaccta taaagagggt gtagcctaca
    15361 agtttgataa ggctacctgc aattgtttag gggcctatcc atttgaagga gatggctggg
    15421 gattagccta tgataaggaa aaccagtgtc tttggatgac tagtggaaat gcttttttgc
    15481 agaagcgaga ccccaaagat tttgctttat tggataccgt actagttgct atagaaagtg
    15541 tccccatctc aatgcttaac gaattagagt atgtagatgg ttatctctat gccaatattt
    15601 ggcaaaccaa tactattgtc aaactccaac ctgattctgg aaaggtggta gccacctatg
    15661 atattagtcc tttgttaaaa gccttaaatc ttgataagtc ccattaccct gaccttaatg
    15721 ttttgaatgg gattgctcat ttagatcaac aacgtttctt aattactggc aaactatacc
    15781 cgctgatgtt agaagtagta ttagattaag atgataattg agaaagaccg atgaaaagtt
    15841 tccctaggtc tttcattttt agcacaaaaa aaacagagtt agttcatatc tctgtttttt
    15901 taaaataggt ttagtagcgt cacagtcagt gagtagcgcc ccctacacgt tttaaatcgg
    15961 aacgatcttt gaaatcgaca atggcaaaaa tagcagcctc aattcctctt gtaatatcat
    16021 cgaggttcat ggcagctgta ttaggtttat caacaacctg ttccatcata aagggaatat
    16081 gcataaaccc agctttggca tttggacaat atttatccac taagtaaagg gcttgataca
    16141 tcaaatgatt gcaaacaaag gtaccagctg tattagaaac agaagcagga agcccagcct
    16201 gatgaatggc agcaaccatc gctttgattg gcaaggttga aaaataagct gctttaccat
    16261 ctgcacgaat aggtgtatca ataggctgat tcccttcgtt atcaggaatg cgagcatcgt
    16321 cttgattaat ggcaacgcgt tctggcgtta gtccagtccg gccaccagct tgcccaatac
    16381 aaaggactgc atcaggttga aagctttcga tatgctgctg gagcacatcg gcagattttt
    16441 gaaaaaccgt tggaacttca atacatttga tttctgctcc atgaatggtt gctggcaatt
    16501 tcttgatagc ttcaagggca ggattaatag cttcaccgcc aaagggatca aagcctgtta
    16561 caagaatttt cataataatg tcctttcaat gaataactga cgctactaac ttagcaaaaa
    16621 agttagtaat aagcaagtaa atacattaag gcaatgtgac taataattaa aacaagggcg
    16681 ataggagctt gctttttgat aactgcattt cgatctttga tctccatcaa agcaacaggc
    16741 aaggcattaa agtttgctgc cattggtgtt aataaggtac cacaaaaacc agcagtcata
    16801 gctaaagcac cggcaataat agggtcagct cctaaagcaa aaacaaaagg aacaccgata
    16861 ccggttgtaa taacggtaaa ggcagcaaaa gcgtttccca taatcatagt aaaaataacc
    16921 atgccaagca cataagcgag aacaccaaag aagcggctat cagcaggtac aatctcccga
    16981 atgagagaag caatcacatc accaacacca gcagctgcaa aaatagctcc taaagctcct
    17041 aagagctgtg gcaaaatacc gcttgtggac acttgttggt tcatccgatt attttcagct
    17101 aaaagagcag atggtttttg cttagtgatt attaaaatag caatagtggc taagatacca
    17161 gcaatcccaa tggcactttt actgaaatca ggaaggatca aggcaagaag tagagaaatc
    17221 atagccatta acataactgg taagaaaatc cagttaccaa tgcggtgagc aatttcttca
    17281 gctttttgtt cattaaaggc tggtaatgtt ccaatacgaa cttgcttaaa caaggttaaa
    17341 agagctagga caatcacgat aaaaccgata actttattag gcaaaaaggc accgccaacg
    17401 aaggtaacac ccagcaatcc ccagaacaga gctgtcccaa aatagacagg gttggtttta
    17461 tctttaaagg tgcagtaggc tgtgtgaaac agttgcaagc cgataaggat atatatcgtt
    17521 tctaaaacaa ggtttgcaat gttagccatt agttatctcc tttctgatgt tgacgttcca
    17581 tttttttctc aaagaggtaa ttgtaaatag caactataat aatagagata atagcaatta
    17641 aaatagaaga aaatgcaatt ttagcttggt taccatcata gccaagctgt tctaaagttc
    17701 cagcgataag taggacacct cctgcgccaa caaaggtatt ttgggcaaag aaattgccaa
    17761 agttttcatt ggcagctgcc atagctttga tgtcatcttt ttctgcatcg gtcaactctt
    17821 ctccaatgtt ggctttagca gcagcttctc ccataggttg aatcaagggg cgaacgaact
    17881 gtggatgtcc ccctagtcga atcgaaaaga gacctgctaa ttctcgaata ataaggtaga
    17941 gagtcaataa acggccgacg gtaagggatt gaacacgttg aatcaactga gtagctcggt
    18001 gtttcagtcc ataagtttct gacaagccga taagcggtaa agtgatgaag aaaatggtta
    18061 gcaaacgttg attggtaaat tcttttccta aaatatctag aaaatcgata aacgaaattc
    18121 ctgaaaccag cgcggtaaca agtcctgcaa caactacggt agcgatagca tcacatttta
    18181 aaataaaccc taacacgata atcacaatac cgattaattt aatccactcc ataaagaact
    18241 cctttttcaa tagtatgacg attataacat tagtcgctag gatttgcaaa aaggctcaac
    18301 ctaatagttc cttttctact taaagtgtta aggaaggtga tactattttg gaaaggtcat
    18361 tatcatttgg tataacgggc ttaaaaagaa agcttcgcgc acagaaagta ggaaacgatt
    18421 gatgagaaaa atgttttaat ttaatgtttt tctttgataa tatagagagg ccttttttta
    18481 gtttcaagga atatttttga aatatatttt ccaataatac ccatgcaaaa gagctgaatg
    18541 cctcccatga ataaaataat agagacagtg ctggcccaac cagagacagg atcaccaaag
    18601 agtatctttc tgataatgat aaataagatg gcgaaaatag aaatcaaaaa gctgaaagtc
    18661 cctgtccagg tagcaatggt taacggcatt tctgaaaagt tgataaagcc atctaaagaa
    18721 tatcggagca actcccaaaa atgccaacga ctttttccat atttgcgttt ttggttttca
    18781 aaactaagat aggtgatacg ataaccaacc cacgaaaaga tcccttttga aaaacgatta
    18841 acctctccca actcaagaat gctatcaacg acttgtctgg tcattaaccg ataatcccga
    18901 actccattga ccatttcagt atctgataag tgtttgatca gtccataaaa tagattcgaa
    18961 cacattgaac gaattagtgg ttctccttgt ctattttggc gcctggtgcc aacgatgtca
    19021 taaccctctt ttagcttagc gtacataact ggcaaaagtt cgggtggatc ttgcagatcc
    19081 acatccatca cagtaatgta attgcctttg gcttctttta gtccagctaa gagtccagct
    19141 tccttgccaa aatggcgtga aaaagacaga taatgcacat ttggaaagcg agtagctagc
    19201 tcacgcaaaa tacccaaggt gttatcctta gaaccatcat caataaaaat gtattcaaaa
    19261 gcaagttggt tggtcatgga cgtttctagt tgatgcattt cttcaaaata aggaagaatg
    19321 tttgcttctt cattaaagca ggggacaatg atagatagta gtgtcataga cttttctttc
    19381 aaatggaatt ttagggcaat tgtaacacaa aagctgagat atttcaaaac tctgttaata
    19441 atgagattgt cccaatcgtc aggttaaaaa ataaaacaac agaagcttta aagcttctgt
    19501 tgttaatagt attagttgtt taaatcaatg acttcaattt tgtagccatc tggatcctga
    19561 atgaaataat agcgggcaga tttgtcagcc agttctttga tatcggtaac tggaaaacct
    19621 gcttgtcgat gttttttatg gtctgcttca aaatgttcag agccaagggc aatatgacca
    19681 tagccatttc ctaagtcgta gtcgccatga ccataattgt aggttaattc taattcataa
    19741 ctttctcctt ctaaagtcag ataaacaaga gtaaattgac tatctggaaa atctttacga
    19801 tagttttcct taaaagggaa ggcgcttgtg taaaaagcaa ccgactggtc aaggtctttg
    19861 acgcggatac aggtatgtaa tgctttcatt ttaatctcct tatttaacga ctgaaatcac
    19921 ttcttcttta ggcttacgaa cttgagcatg tttgggatct cgcaggcgat agcctaggga
    19981 taacatgcta gcaatgcctt ctttttctaa atcaatcaca ttatgcttag ctaggatatg
    20041 attgacctta tcataatgaa agccttcaat agggcaagta tcaatgccca aaagagcagc
    20101 tgtcatcatc atattaccaa gagcgatata ggtttgttta gctgtccaat caaagagtgc
    20161 ccgaggatta tctgccatat ccatgtcctc tttttggaaa gattcataga gttttaggcg
    20221 gctgttgaga ccatcacctt ctttgatacc acgtcgtaaa aggctatttt tgatagcagg
    20281 gctatcgtat cttgcatgtt tttcagctat taaaagaata aagtgactag ctgtctctag
    20341 ttgatactgg gccccccagg caaagggctt gatttcttct ttaataggct tgttgtctaa
    20401 aacgacaaag cgccagcctt ctaagccaac agaagaaggg cttaaccaag cagcatcaag
    20461 gataagggct aaatcctcat cagaaatctt ttcttcctta taaacacgaa cggctgttct
    20521 aaagtgtagt gcttgctgta tttgatgatg aatggtttga tccatacgaa taccttcctt
    20581 tcgcgagggt ttgtttaacc agatggtagg ggcttagcct tcaaaataaa gcaactcttt
    20641 tggagtatgg gtaaagactt caaagccatt tttagtgaca tggccacagt cttcgatgcg
    20701 cacaccgacc tttcctggaa tgtaaatgcc tggttcaaca gagaagcaca taccttcttc
    20761 aaggaccaag tcattgctag ccataatgga gggaaattcg tggacatcca tgccgatacc
    20821 atgtcccaag cggtggttga agtactcacc atatccagac ttttcaatca cttgacgagc
    20881 aacagcatct acttgagcag ctgttacacc tggtttgata aagtcaatag ccactagttg
    20941 tgcctctagg cagagattgt agatatcgat tttaaattgg tctggctgac caacagccac
    21001 agttctggtc atatcactgg tgtagcctaa ggtctcaaca cctaagtcaa aaagaagtag
    21061 ggcattgttt tcaatgttat ttgttccagg aatgccatgg ggattggctg cattatttcc
    21121 agttaagacc atagtgtcaa agctcatttt atgaatgcct tgttttttca tttcaaactc
    21181 aatttgagcg atgacgtctg tttcggtagc atctaaggaa atattgtcaa aaccaacttg
    21241 aacagcctta tcagcgaact gcccggcaat catcatttta ttgatttcat cagctgattt
    21301 gatgaggcgc atgccttgga cataaggggt tagattgtta aattgccctg aaaagatagt
    21361 ctgcaagcca tggaacttat tgacgttaag gtggtcaaat tcagcataaa tggttttggc
    21421 tgctgtattt ggtaaaacgg ccttgatctt ttcccaagga ttttcagaat caacataacc
    21481 aaacacagga aatgatatgg cctggctagc tctggccacc tcaagggcag gtacaaaaag
    21541 cacgggagct aaatcatggt agacaaacaa gaacagttgt ctttcatgtg ggtcacagaa
    21601 gaatccagtt aaatagttaa tcgtaacagg atctgagaaa atcgccaatt cagctccctt
    21661 ttggtcaaga tacaaacgta tttggtctaa ttttgtcata ggctaacctt ctttctactt
    21721 actatttttt caaaaaaagc ttaaaaaatc aagcttcgag ttaatttttg aaaacttttt
    21781 caaaaattaa cctataacac ttgaaagtgg ttacaaatca tgctaaaata ctttttgaaa
    21841 gcgtcatttt caaatagaaa ggaagataaa aaatgaatac agatgatacc attacaattt
    21901 atgatgttgc ccgtgaagct ggtgtttcaa tggcaaccgt tagtcgtgtt gttaatggca
    21961 ataaaaatgt taaggaaaat acacgtaaga aagttttaga agttattgat cgccttgact
    22021 atcgtcccaa tgctgtcgcg cgtggtctcg ccagcaaaaa gacaacaacc gttggcgttg
    22081 tgattccaaa tattgcaaat agttattttt ctatcttagc taagggtatt gacgatatcg
    22141 ccgctatgta caaatataat attgtgcttg cctccagtga tgaagatgat gataaggagg
    22201 ttaatgttgt caataccctt tttgccaagc aagtggatgg tattattttt atggggcatc
    22261 acttgacaga aaaaatccgg gccgagtttt cacgctctcg tactccagtt gttttggcag
    22321 gaactgtaga tcttgatcat caattaccaa gcgtcaacat tgactataga gccgcggtgt
    22381 caaacgttgt tgatatttta gctgaaaatc ataagtgtat cgcttttgtg tcaggaccac
    22441 tcattgatga tatcaatggt aaagtgcgct tagcagggta caaagaaggg ttgaagcaca
    22501 ataagcttga cttcaaagaa ggtcttgttt ttgaagcgaa ctattcttat aaagaaggat
    22561 ttgaattggc gcagcgcgtc attaactcag gagcaacagc ggcttatgtg gctgaagatg
    22621 aattggcagc aggcctttta aacggcttgt ttgaagcagg taaacgcgta ccagaggact
    22681 ttgaaatcat cactagcaac gattcaccag tggttcaata cactcgtccc aatttgagtt
    22741 ctatcagtca acctgtttat gacttaggtg ctgttagcat gcggatgttg actaaaatca
    22801 tgaacaaaga agagttggaa gaaaaagaaa ttcttttgaa tcatggtatt aaaaaacgtg
    22861 ggacaactaa gtaactgtga cttatgagca ccatctaaca aaagtatcac aatagactgt
    22921 ttcttcaata ctaaaatcag ccttagggct gatttttgca tgtcttagtg aaaaatacta
    22981 taaaatgagc tataatagaa cttatgaaag tcttattgta tttagaagca gaaaattatc
    23041 taagaaaatc aggaattggt cgagcgatta agcatcaggc taaagccttg tcacttgttg
    23101 gtcaacattt tacgactaat ccaagagaaa cttatgattt ggttcatctc aatacctatg
    23161 gtttaaaaag ttggctgctg atgataaaag cacaaaaagc tggtaagaag gttatcatgc
    23221 atgggcattc tacagaagaa gattttagaa attcttttat tttttcaaat ctattatctc
    23281 cttggtttaa aaaatacctt tgtcactttt acaataaggc agatgctatc attaccccta
    23341 ccctatattc taagtctttg attgagagtt atggagtgaa gtcacctatt tttgcagtgt
    23401 caaatgggat tgatttggag cagtacggag cagatcctaa aaaggaagca gcttttcgtc
    23461 gctactttga cattaaagag ggtgaaaaag tggttatggg agcaggatta ttttttctga
    23521 ggaaaggaat tgatgacttt gtcaaagttg cccaagctat gccagatgtt cgttttatct
    23581 ggtttggcga gaccaacaaa tgggtcattc ctgctcaagt tcgccaaatg gtcaatggta
    23641 accacccgaa aaatcttatt ttcccaggat acattaaagg ggatgtttat gaaggtgcca
    23701 tgactggtgc agatgccttt ttctttccaa gtcgtgaaga aacagaaggc attgttgtct
    23761 tagaagcctt ggccagtcgc cagcaccttg ttttacgtga tataccagtt tactacggat
    23821 gggttgatca aagtagtgcg gaattagcaa ccgatatacc aggttttata gaagctctga
    23881 aaaaagtctt ttctggtgcc agcaacaaag ttgaagctgg ttacaaggtt gcccagagtc
    23941 gtcgcctaga aacggttggc catgccttag tagatgtcta taaaaaagta atggagttat
    24001 aagatgcgta taggtctatt cacagatacc tattttccac aagtttcagg agtcgctact
    24061 agtattcgta cgttaaaaga agagctagaa aaagaaggtc acgaagttta tattttcacc
    24121 actactgata gagatgtcaa acgctttgaa gacccgacca ttattcgact gccaagtgtt
    24181 ccttttgtgt catttacgga tagacgtgtg gtttatcgtg gcctcatttc gtcatacaaa
    24241 attgcaaaac actataatct tgatattatt catacgcaaa ctgagtttag cttaggctta
    24301 ttagggaaaa tgataggcaa agctttgcga attcctgttg tccatactta ccatacccaa
    24361 tatgaggact acgtgagtta tattgccaac ggaaaaatca ttcgaccaag tatggtcaaa
    24421 cctcttctta ggggctattt gaaggatttg gatggggtta tctgcccaag taggattgtc
    24481 ctcaatcttc tagaaggtta cgaagtgact atccctaagc gggttatccc aacaggcatt
    24541 cctttggaaa aatatattcg tgatgacatc acagcagaag aagtaaccaa cttaaaagca
    24601 gaattgggca ttgctggtga tgaaaccatg ttattgagtt tgtcacggat ttcttatgaa
    24661 aaaaatattc aagctatcat caatcagatg ccagctattt tggctgaaaa tgccaagata
    24721 aagcttatta ttgtaggaga tggcccctat ttgcaagatt tgaaacactt tgcgatgcag
    24781 ttagaggttg acaaacacgt gacctttaca ggcatggtgc ctcatgataa ggttgctctg
    24841 tactataagg cttgtgattt ctttatctca gcatcaacta gtgagactca gggcttgacc
    24901 tatattgaaa gtttggctag tggcactcct attattgctc atggcaatcc ttatttagat
    24961 gatgtggtga ctgataaaat gtttggcact ctttattacg ctgaaacaga tttaactgat
    25021 gctattattg atgccatact aaaaacacca gttatggata agcggttatt agcaaaaaaa
    25081 cgttatgaaa tctcagcaca gcactttgga aaatctattt acacgttcta tttagatacg
    25141 ttaattgcta gaaatagcaa agaagctcaa aagctgagac tttatcttaa tcattctggt
    25201 aaaagtagtt ctctaaaatt agtgcaaggt gctattcact tgcctaaacg tgctgctaag
    25261 gtcacagcta tcacctcagt aaaagtagtc aaggctccta tcaagctggt ccatgctatc
    25321 aaagattttc tggattaagg cttgtaaatt tttgaaaact tgctataata atcttgaaga
    25381 caagttagat tggacagaat cttttaagcg agtgctggtt ggtggaaaga gcacaaggat
    25441 agctaatctg atactccttc ctggttttta tgcaaacata aaagggtggc ccccttatcg
    25501 ccactagagg tgcaaggcat gttttttgtc ctgcataaat taaggtggaa ccacgctgat
    25561 tgcgtccttt gggatgcggt cagttttttt gttttggagg ggattagcct tgtttttaag
    25621 gactagggag ggtagattcg tgaataaggt attgttcttc tacgtggtgg tattgagcta
    25681 ttcactaacc tcttgtccct aatttgtctt actatccatg ttttagtgga aatcttaact
    25741 gacttaaagg agaaagcaaa tgattaagat tacttttcca gatggcgccg tgcgtgaatt
    25801 tgaatctggc gtaacaacgt ttgacattgc tgagtccatc agcaaatcat tggctaaaaa
    25861 agctttagct ggtaagttca atgatcaact cattgacacc actcgtgcca ttgaagaaga
    25921 tggcagtatt gaaatcgtga cacctgatca caaagatgct tatgaggtgc ttcgccactc
    25981 tgcagcccat ttatttgccc aagctgcaaa acgcttgttc ccaaatcttc atttgggtgt
    26041 gggccctgcc attgcagaag gcttttacta tgatacagat aatgctgaag gccaaatttc
    26101 aaacgaagat ttgccacgta tcgaagctga aatgcaaaaa atcgtgacag aaaattaccc
    26161 atgtatccgt gaagaagtga ccaaagaaga agctttggaa ttgttcaaag atgatccata
    26221 caaggtagaa ttgattaatg agcatgctgg tgctggcttg acggtttacc gtcaaggtga
    26281 atttgtggat ctttgccgtg gccctcatgt gccatcaaca ggccgtatcc aagtcttcca
    26341 cttgctcaat gtggcgggtg cttactggcg tggtaacagc gacaataaca tgatgcaacg
    26401 tatctatgga acagcttggt ttgataaaaa agacctcaaa gcttacttga ctcgtctaga
    26461 agaagccaaa gaacgtgatc accgtaagct tggtaaagag cttgacttgt tcatgattag
    26521 tcaagaagtt ggtcaaggct tgccattttg gttaccagat ggagcgacta ttcgtcgtac
    26581 acttgagcgc tacatcacag ataaagaatt agcatcaggc taccaacacg tttacacacc
    26641 accacttgct tctgtagaat tgtacaagac atcaggtcat tgggatcatt atcaagaaga
    26701 tatgttccct gtaatggata tgggagatgg tgaggagttt gttcttcgtc caatgaactg
    26761 cccacaccac attcaagtgt ataaaaatca tgtgcgttct tatcgtgaat tgcctattcg
    26821 tattgccgag cttggcatga tgcaccgtta cgaaaaatca ggtgccctat caggacttca
    26881 acgggtacgt gaaatgacct tgaatgatgg ccacattttc gtaacaccag agcaaatcca
    26941 ggaagaattt caacgtgccc ttcaattaat cattgatgtt tatgctgact tcaacttgac
    27001 tgattatcgt tttcgtttgt cataccgaga ccctaacgat actcataaat attatgacaa
    27061 tgatgagatg tgggaaaatg ctcaaagtat gcttaaagct gcacttgatg agatgggtgt
    27121 agattacttt gaagcagaag gtgaagcagc cttttatggg cctaagcttg atattcaggt
    27181 gaaaacagct cttggtaacg aagaaaccct atcaaccatt cagttggact ttttgttgcc
    27241 agaacgcttt gaccttaaat acattggtgc agatggcgaa gaacaccgcc cagtcatgat
    27301 tcaccgtggt gttatctcaa ctatggaacg tttcactgct atccttattg aaacttacaa
    27361 gggtgctttc ccaacttggt tggcacctca ccaagtaacc gtgattccaa tttcaaatga
    27421 agcccacatt gactatgctt gggaagtagc aaaaaccctt cgtgaccgtg gtgttcgtgc
    27481 agatgtcgat gaccgcaatg aaaaaatgca gtacaagatt cgtgcgtctc aaaccagcaa
    27541 aattccttac caattaattg ttggggacaa agaaatggaa gacaaatcag tcaatgttcg
    27601 ccgctatggt agtaaggcga ctcacactga atctgtggaa gaatttgtgg aaaacatctt
    27661 ggcagacatt gctcgcaagt cacgtccaga tgcacaagct taatcaatgg caaaaagagg
    27721 tccttatggg cttctttttc tttcttaaaa aattaagcaa acgattgcac cgtctttttt
    27781 atggtgttat aataaattgt ttagtaacgg tgataagacg ataacagaaa cgaggaatgg
    27841 taaatggtca tgatagaagt gtcccacctt caaaaaaatt tttccaaaac cattaaagag
    27901 ccaggattaa agggagcctt gaagtccttt gttcatcccc aaagagaaat ctttgaggcg
    27961 gttaaggact tatcttttga ggtacccaaa gggcaaattt taggttttat tggcgccaat
    28021 ggtgcaggaa aatcaacaac gattaagatg ttaacaggta ttttaaagcc tacatcaggt
    28081 tactgtcgca ttaacggaaa gattccacaa gataatcgtc aagattatgt gagagacatt
    28141 ggagctgttt ttggtcaacg aacacaacta tggtgggatt tggccttgca agagacctat
    28201 gtcgttttaa aagaaattta cgatgtgcct gaaaaagcct ttcgcaagcg catggatttt
    28261 ttaaatgagg ttttggacct gaatgagttt attaaggatc ctgtccggac cttatcactc
    28321 ggtcagcgga tgcgagctga tattgctgct tccttgttgc ataatcctaa agtattgttt
    28381 ttagatgaac caacgattgg acttgatgtt tctgtgaagg acaacattcg gcgtgccatt
    28441 actcaaatta atcaagaaga agagacaact attttactga cgacccatga tttgagtgat
    28501 attgaacagc tctgtgatcg gattatcatg attgataagg gccaagaaat ctttgatggc
    28561 acggtgaccc aactcaaaca aacatttggg aagatgaaaa gcttatcttt tgaattggaa
    28621 gcgggacagg aagaagttgt ttctcagttt cttggtttgc ctgatataac tgtggaacga
    28681 cacgaattaa ctttggaaat tcaatatgac agttcgcgct atcaaacagc ggacattatt
    28741 caaaaaacca tagctgattt tgcggtcagg gatttgaaga tgactgatgt agatattgag
    28801 gatattgttc gtcgtttcta ccgaaaggag ctttaagatg tggtcatttt ggaaacgtta
    28861 tagccccttt ctcagtgcag gaattcagga attaataacc tatcgcgtta atttcttttt
    28921 gtatcgtatt ggagatgtca tgggggcttt tgtggcttat tacctctgga aggctgtttt
    28981 tgattcgtct aagcaatcct taatcaatgg ttttactctg tctgacatga ccttttacat
    29041 tatcatgagt tttgtgacca ctttactgac caaatcagac agctccttta tgattggcga
    29101 agaagtgaaa gatggttcta tcatcatgcg cctgcttcga ccagttcatt ttgcagcctc
    29161 ttacctcttt atggaaattg gttttcgctg gattgtgcta atgagtgtcg gctttccttt
    29221 tttagtggtc ttatctggta ttaaagtcat ggcggggctg tctatcttgc aagtcctagc
    29281 cagcagttgt ctgtatttag ttagcctttt attagctttt ttaatcaatt tttatttcaa
    29341 catttgtttt ggcttttcag catttgtttt taaaaacctt tgggggtcta atttattgaa
    29401 aaatgccttg gttgccttta tgtcaggcag tttgattcca ttggcttttt ttcctaaaat
    29461 ggtttctata gtgcttagtt ttctaccgtt atcctctttg gtttacacgc cagttatgat
    29521 tgtcattggg aaatacagcc ttagtcagat aatggttgcc ctaagtttgc aaatcttttg
    29581 gttactggtg atggtagtct tatcgcaagt catctggaaa aaggttcagt accatttaac
    29641 gattcaagga gggtgagatg gcaaaattga gacgtatgca cgctattttt atcaaacaat
    29701 acctcaaaca aattatggaa tacaaggttg attttgtggt gggagtgcta ggtgtttttc
    29761 tcactcaggg acttaatctg ctctttttat cggtactttt ccaacatatc ccttctttag
    29821 aaggctggac gtttgaacaa attgccttta tttatggctt ttctttgatt ccaaagggaa
    29881 ttgatcacct cttttttgac aatctttggg ctttagggca acggcttgtc cgcaaaggag
    29941 aatttgacaa gtacctcacc agaccaatta gtcctttgtt tcatgttttg gttgagacct
    30001 ttcaagtgga tgccctgggt gaattattgg ttggggttat cttattagtg accacggctg
    30061 gtagtatcgt atggactctt cctaaggtgc ttttgtttat tttggttatt ccttttgcga
    30121 ccttgattta cacctcttta aaaattgcga ccgccagcat ttctttttgg accaagcagt
    30181 ctggggcagt tatctatatt ttttacatgt tcaatgactt ttccaagtac cctatgtcca
    30241 tttaccattc ctttttgcgg tggttaatca gttttatcat tccctttgct tttacagctt
    30301 attaccctgc ttcttatttt ttaacaggtc aacacctgct ctttaatatt ggaggcttgg
    30361 tagtggtgtc gttactggtt ttggccttgt cgcttaagct ttggaaatgg ggcctagatg
    30421 cttatgaaag tgcaggttcc tagagagact cttaaacaaa ttgaaaatcg tttaggtcag
    30481 aatatgaaaa cctagataat gaaggaaggc agctgttgta aaaacggctg cttttttttg
    30541 tgatggttta ccaagcgtag gcattggttg gaaccatcat cagcagattg gtctaaatta
    30601 agggcggtaa aataacataa gcagatatat ttcaagtggt tgcatgcaat tgctagataa
    30661 catttatgga actttgagat cttttgcctt tatgagacct gtcaaatcta gtaacgatac
    30721 cagcttagca gatgccttgc aggctgatac agacatggct tttattttag ttagaggcat
    30781 taactatcaa taaatagtaa agacaaggtt tgtcaatacc ataaaaaagt taagtgtcac
    30841 agcttagctt ttttatggtg tctaattctt tattctatat aagattttct tttcttagga
    30901 atggttttcc agtataataa agtcaccctg tatcaaaaac aaaggttaca gtttgaatgt
    30961 taaagcaaca aagtaagtga cttctacgac aatagttgtg ggacttgaca tttcaatgac
    31021 gttagagaat tttatagatt ttattgatgg cacactagcg agactaaaga gattctctaa
    31081 attaatttag gtgtattttt agcagttatt agttcaaaaa agaaaaccat cagaaataat
    31141 tatataataa ccatttaagc acatcgaaaa taaaggaggt caatataatg acggatgttt
    31201 atattgctgc aggccttaga acccctatcg gtttagtagg gaaacaattt gctaaagaac
    31261 aaccagaaat ccttggagca aagctcatca acgctttaca aaataagtat ccagttccca
    31321 ttgaccaagt gatttgtggc aatgccgtcg gaacgggtgg taatattggg cgcttgatga
    31381 ccctgtattc tcatttagga gaatctgtct ctgctttgac ggttgatatg cagtgtgcat
    31441 cagctggtgc agctctttcc gtgggttatg ctaaaatcaa ggcaggtatg gcaagcaacc
    31501 ttttagtggg agggattgaa agcagctcct tacaacctga atcggtatat gcttcagctg
    31561 attggcgcca aggagcctac aaggtagctc agttttctcc agatagtatc agcccttttg
    31621 cgatgattga aggggcagaa cgagtggcaa gagagcatgg ctttacaaag gagtatttga
    31681 atcactggac attgagaagt catcaaaagg ctagttattg ccaggagcaa gccctgttag
    31741 ctgatcttat tctagacctg ccaggagcca gtgatcaagg tattcgaccg cgcttgtcaa
    31801 gtaaggtgtt gtcaaaggta ccccctatct taggagaaga tcacgtgatt agtgcagcca
    31861 atgcctgcct aacccatgat gctgctgcct ttttacagct aagtagtcag ccttcagctt
    31921 ttaaactgat tgatgtggta gaagtagcag gagacccaca gcgtagccct ttaatggtga
    31981 ttaaggctag tcaagtcctt ttggaaaaac atggtctagg gatggcagat atgacagcga
    32041 ttgaatggaa cgaagccttt gccgttattg atggtttatt tgaaacccat tacccagatt
    32101 tattggaccg ctataatata tttggtggtg cattagctta tggtcatccc tatggtgcct
    32161 cagcagccat tataatattg cacttaatga gggctttaga gattaaaaat ggacactatg
    32221 gaatagctgc tatagcagca gctggtggcc aagggtttgc tgttttacta aaatatcata
    32281 aggagtttag tcatgctgac aaagctagaa tattgggcaa agcaatgccc aaataaaaaa
    32341 gctatcgtag ccgatcagat aagcttgact tatcaagagc tatggcaagc ggttttaata
    32401 aaggatcaga caataaaaga cagtgtacca tatatcatca gtcatagtcg ctatcttaat
    32461 cagttactct cttttttacg aggattaaaa gaaggcagtt gtcctatcat tttacaccct
    32521 aatatctcag gtacatttca gcagcaaata aaacacgtcg atggtgagct tcttaaaaaa
    32581 gctgattttg ctgttttgag ttcgggaaca actggcaaag caaaattatt ttggcgttgt
    32641 ctatcaactt ggacgagact ttttgattat caaaataagg tgtttggtat gactggtaat
    32701 agttgtctat tcttacatgg tagttttagt tttactggca atcttaactt agcattagct
    32761 caactatggg ccgggggatg tctggtttta agccaaaagc tgtctttgaa aacgtggtta
    32821 agcttatggc aagaaaaaaa ggtgagtcac ctttaccttt taccgaccta cctaaatcgc
    32881 ttgctacctt atttgactaa gaacaacatg actgcgactc acttattgac ctcttcgcaa
    32941 atgatatcac aagaattact aagacattat tacaaaaaat ttcctcagct tgaaattgtc
    33001 attttctacg gggctagtga attatctttt ataacgtggt gtaatggcag agctgctgtc
    33061 aaaattaatg gtttggtagg acagcccttt cctgatgtgt ctatcagttt caaagataag
    33121 gaaatttttg tagagactcc ttatagtgta gaaggaatgt cacaaccata cagtgtctca
    33181 gacttaggaa aaatgagccc agcgggttta attttagaag ggcgacaaga tgactgggtt
    33241 aatcaacgag gagtgaaatg ccacctgcct agcctagttg aactcgctca ccaagcgcca
    33301 aacgtcaaag aagcgcacgc tttaaagata ggaaaaggag aaaacgaaac cctaatatta
    33361 gtattagttt tgactaagaa ggattgccta gcacctatta aagacttttt agctctttat
    33421 ttaaactctg gacaacttcc caagtactat cttgtgattg attgtttgcc tttaaaagat
    33481 aatggtaaaa ttaatcgaga agttctttta aataaaatac ccaaacagtg gcttagttaa
    33541 ccacttcagg tttcgttttg cttatagtga gatacaagtg acgattcagc tttagatgtg
    33601 ttataatatg gtcaaaaaag atgaggagaa ggccatgaaa caagctatct tagatcgcta
    33661 tcaggccttg aaatgctatc aaaacgcagg tttatcaaac caagcatttc gtgctatagc
    33721 taaagagcct atcattgata atcgacttgg ttctccaact ttctgggtaa tatggcctat
    33781 tgaaaaagaa aaccaatcag ctaagcagtt actaacgttt cttcttgatt tggtagagat
    33841 gccatttgag ctaagtggtc agttgcatga aactcaaacg ctactcaccc gattccatcc
    33901 atctctattg ccagatcata tgttttggaa agagttggct agcttagttg atcaagcttt
    33961 tcctggcaaa acactaagcc aagcaggtga gcttgaaaag cgtttgcatc aatttcgtta
    34021 tatcatctct agtcaacaag ctcaatctat cagaaaccat tataaaatga ttgagatgac
    34081 agacgcccaa gctctggccc tttttttgag gtctaaaaaa gggccttgtt tgtggcggca
    34141 agcaccagat tataccttga tggattctgc caggttacat aataaactaa gatttgaaga
    34201 taataaagtc atttttccaa gtcaagaagt ttcctataat ataaaagtac ttctttggtt
    34261 tcataccgaa tttattctag atagcacagg cttttttctt aatgaagttg atgcagaagt
    34321 agtgaccgaa aaaggaattg tcaatggtgc atcttttaac tatggcacag atggtcctag
    34381 acattgggat ttagatgttg atccgattag ccgtcacgat cctcagttta gaagagacac
    34441 ccttaaagga tttcgaacgc caaaaagagt atttcgtcag tggtttagag ctcaaaaaga
    34501 tgactttatg ttctcttatt ttaatgccaa aggacttttt gcttaccaca acaagagtag
    34561 ttttgccaga gtcaaaaaat ctgctaagca attcaaacgc caaattcatc ctataaaggg
    34621 ttggttttag aaagtactct atttgtaacg gtagtcgtca ttgtca
//
LOCUS       DAUCWO010000010        34543 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUCWO010000010 DAUCWO010000000
VERSION     DAUCWO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34543
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.010"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            517..993
                     /locus_tag="VGY20_000470"
     CDS             517..993
                     /locus_tag="VGY20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ4273352.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            1051..2232
                     /locus_tag="VGY20_000471"
     CDS             1051..2232
                     /locus_tag="VGY20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ4273353.1"
                     /translation="MSLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            2222..3469
                     /locus_tag="VGY20_000472"
     CDS             2222..3469
                     /locus_tag="VGY20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ4273354.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDLEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(3528..5180)
                     /gene="fbp54"
                     /locus_tag="VGY20_000473"
     CDS             complement(3528..5180)
                     /gene="fbp54"
                     /locus_tag="VGY20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ4273355.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRLQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKRLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLF"
     gene            5534..6532
                     /locus_tag="VGY20_000474"
     CDS             5534..6532
                     /locus_tag="VGY20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4273356.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANQQNITIGIL
                     QLVTHEALDDIKRGIEDQLKKQKPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            6483..6701
                     /locus_tag="VGY20_000475"
     CDS             6483..6701
                     /locus_tag="VGY20_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273357.1"
                     /translation="MLLTGKIFLQLLLLRINTKTCDIKGQEKTIKNTLTEVNLDAKSD
                     NRLEDVMVIFIIFSFKMLYFRHVKNLAF"
     gene            6878..7747
                     /locus_tag="VGY20_000476"
     CDS             6878..7747
                     /locus_tag="VGY20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273358.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCFPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            7744..8502
                     /locus_tag="VGY20_000477"
     CDS             7744..8502
                     /locus_tag="VGY20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273359.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            8706..10367
                     /locus_tag="VGY20_000478"
     CDS             8706..10367
                     /locus_tag="VGY20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ4273360.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            10500..11285
                     /locus_tag="VGY20_000479"
     CDS             10500..11285
                     /locus_tag="VGY20_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ4273361.1"
                     /translation="MANIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRAAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            11315..11641
                     /locus_tag="VGY20_000480"
     CDS             11315..11641
                     /locus_tag="VGY20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273362.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            11687..13594
                     /locus_tag="VGY20_000481"
     CDS             11687..13594
                     /locus_tag="VGY20_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ4273363.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFLTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVETG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            13879..14847
                     /locus_tag="VGY20_000482"
     CDS             13879..14847
                     /locus_tag="VGY20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ4273364.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            14906..15904
                     /locus_tag="VGY20_000483"
     CDS             14906..15904
                     /locus_tag="VGY20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ4273365.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            16089..17498
                     /locus_tag="VGY20_000484"
     CDS             16089..17498
                     /locus_tag="VGY20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ4273366.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVLVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKVAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEYKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKLEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            17829..19592
                     /gene="lpdA"
                     /locus_tag="VGY20_000485"
     CDS             17829..19592
                     /gene="lpdA"
                     /locus_tag="VGY20_000485"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ4273367.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(20265..22682)
                     /gene="hylA"
                     /locus_tag="VGY20_000486"
     CDS             complement(20265..22682)
                     /gene="hylA"
                     /locus_tag="VGY20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ4273368.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMIGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASTNITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     NDTFVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGQWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYGKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            22915..23904
                     /locus_tag="VGY20_000487"
     CDS             22915..23904
                     /locus_tag="VGY20_000487"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ4273369.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIGRNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(24013..24804)
                     /locus_tag="VGY20_000488"
     CDS             complement(24013..24804)
                     /locus_tag="VGY20_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ4273370.1"
                     /translation="MTYTSLKSPENQDYIYDLTIAHLYGNLMNTYGDNGNILMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLSSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(24804..26147)
                     /locus_tag="VGY20_000489"
     CDS             complement(24804..26147)
                     /locus_tag="VGY20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ4273371.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTTITNHSSEFIIDGQDYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIIETIEKQEAKHAYILATYTAMLEFRSLLADRHVVEKEMI"
     gene            26254..27105
                     /locus_tag="VGY20_000490"
     CDS             26254..27105
                     /locus_tag="VGY20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ4273372.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            27102..28058
                     /locus_tag="VGY20_000491"
     CDS             27102..28058
                     /locus_tag="VGY20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ4273373.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSYPGTVSVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            28112..29467
                     /locus_tag="VGY20_000492"
     CDS             28112..29467
                     /locus_tag="VGY20_000492"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ4273374.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            29591..30232
                     /locus_tag="VGY20_000493"
     CDS             29591..30232
                     /locus_tag="VGY20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273375.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLTD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            30295..31425
                     /locus_tag="VGY20_000494"
     CDS             30295..31425
                     /locus_tag="VGY20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ4273376.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FESYDIHDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLEQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGISFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            31435..32187
                     /locus_tag="VGY20_000495"
     CDS             31435..32187
                     /locus_tag="VGY20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ4273377.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCAFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQIISDGQLNAQAMIEWRAIKQTESEID"
     gene            32187..32951
                     /locus_tag="VGY20_000496"
     CDS             32187..32951
                     /locus_tag="VGY20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ4273378.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLTNQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            32951..33583
                     /locus_tag="VGY20_000497"
     CDS             32951..33583
                     /locus_tag="VGY20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ4273379.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
     gene            34100..34543
                     /locus_tag="VGY20_000498"
                     /pseudo
     CDS             34100..34543
                     /locus_tag="VGY20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370026.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
BASE COUNT        10624 a         6002 c         7293 g        10624 t
ORIGIN      
        1 atatttattg atattttaat ataaaatata tgaggagata agagattggg aggttgtgac
       61 aatcgagcgg atatcagatg ttatggcaga actttctgag tctgaataat tttagttcac
      121 caaagaatca aaaaagaacc attggggcaa aattggggca taagtttaga acttttgtcg
      181 tttcttgtgt cgatacaata ccggtttaat actaacaaat gcttttatta tcgactttgt
      241 aaccattatt gttccttgtg ttttttcggg aataaactca agaagtgatt aaataaaaca
      301 ttaaagagcc ttgtcatatc aattatcgtt gatatgacaa ggttcttttg atagtttaaa
      361 ttgatgtttt tcattgtagg ctcttatcta cactttttta aggcatcttt agaaggtttt
      421 gagcgtgact aagtattcac actttgactc ataaaatgat agcatatttt ctagagattg
      481 gtataataga aacaaagatg gaaaggaaag agatagatga ctcaattagc aaccatttgt
      541 tacattgata atggcgattc tttgctatta ttacaccgca ataaaaaaga aaatgatgtc
      601 cacaaaggca agtggatttc agttgggggt aagctcgagg ctggtgagac cccagatgag
      661 tgtgcccgtc gtgagatttt agaggaaacc catttgactg ttacagagat ggcctttaaa
      721 ggaatcatta cttttccaga atttacccct ggtcatgact ggtataccta tgtttttaaa
      781 gtaacaggat ttgaaggaga cctcatttcc gatgaagagt cacgagaagg aaccttggaa
      841 tgggtacctt acgatcaagt gttggaaaag ccaacttggg aaggtgatta tgacattttt
      901 aaatggattt tagaagaccg tccttttttc tctgccaaat ttacctatga ccaaaacaac
      961 caactcatgg ataaatctgt gaccttttat tgatttaccc aggttaccag taaaacttat
     1021 cattttctca aagaaaggaa ccctatctta atgtccttaa tacctgaaaa gtctagaaca
     1081 gatagcttat tttacaaatg gtttttaaat aatcaagcta ccatggcttt agtaataaca
     1141 cttttagcct ttttaacgat ttttgttttt actaagatct cttttttatt tatgccagtg
     1201 atttcttttt ttgcggtcat catgttgccc ttggttattt cgaccattct gtattatctg
     1261 accaaaccct tagttgacct tattaatcac ttgggcccga atcgtaccac ctctattttt
     1321 attgtttttg gtttgattac cttacttttt gtctgggcga tttcaggctt tgttcccatg
     1381 gtacagaccc aacttaccag ttttattgag gatctgccca aatacgtggg caaggtcaat
     1441 gaagaagcca ataaacttct tgaaaatgaa tggttagtca gctacaagcc ccaactccaa
     1501 gacatgctta cccatacaag tcaaaaggct ttagattacg cgcaaagttt ttcaaaaaat
     1561 gctattgact gggctggtaa ttttgctggt gccattgctc ggattaccgt ggctattatt
     1621 atttcgccct ttattctctt ttatttttta cgagacagta gccacatgaa aaatggcctt
     1681 gtgaatgtct tacctctcaa attacgggtg cctatggttc gagtcttggg agatattaac
     1741 aagcaattat caggctatgt ccaagggcag gttacggttg caattgttgt cggctttatg
     1801 ttttctatca tgtttagcct tgtgggactg aaatatgcca tcacttttgg gattattgct
     1861 ggctttctta acatgatacc ttacttagga agtttccttg ccatgatacc tgttgtgatt
     1921 atggctatgg tacaaggccc atttatgttg gttaaggtgc ttgttatctt tatgattgag
     1981 caaaccatcg aagggcgttt tgtagcaccg cttgttttgg gaaataagct gagcattcat
     2041 cctattacca ttatgttctt gttattgact gcaggctcta tgtttggtgt ctggggtgtt
     2101 ttccttgtca ttccaatcta cgcttctgtc aaagttgtga ttaaagaatt atttgactgg
     2161 tacaaaaaag ttagcggttt gtatgacgaa gaagtattgg tgattgagga ggttaaggac
     2221 catgttaaat agtgaaaaaa tgattgcttc cttggatcag caagacctag cgcacgctga
     2281 aaaatatttc caaaaagctt taaaagaaga tgatgcagat agtttaatcg ccttaggaga
     2341 atacttagaa agtataggtt ttttgccaca tgccaagcgt atttatttgc agttagctga
     2401 tgattaccca gaactcaata taaatttagc tcagattgca gcagaagacg acgccattga
     2461 agaagctttc ttgtatttgg ataaggtttc aaaagatagc cctaattatt tgtcagctct
     2521 cttagtcatg gctgacctct atgacctgga aggactgaca gaggtggcgc gtgaaaaatt
     2581 gcttcaagca gtggccatca gctcagagcc actggtcatt tttggtttgg cagaaatcga
     2641 catgtccttg cagtatttta aagaagctat tgattactat gcacagttag acaatcgtca
     2701 aatccttgaa ttgacaggta tttccactta tcaacgcatt ggacgagcct atgctagctt
     2761 agggaaattt gaggcagcca tcgaattctt ggaaaaggcc gtggccattg aatacgaaga
     2821 tgaaactgtt tttgaattgg cgaccctcat gtatgaccaa gaaaattatc aaaaagccaa
     2881 tctttacttt aaacagttgg aaaccatcaa tccagattat ccaggttatg aatatggtta
     2941 tgctttgagt cttcatgaag agcataagac gtcagaagct ttgcgcttgg ttcaacaggg
     3001 cctacgtaaa aatgcatttg acagtcaatt attattacta gcatcgcagc tttcttatga
     3061 attacacgat aggcagaatg ctgaaaatta cctgcttcaa gcaaaggaag tagctgttga
     3121 tgacgaagag attctcatgc gtttagtgac cctttatttt gatgccgagc gttttgagga
     3181 ggttattgct cttaatcgtg agacgattga taatgtctta accaaatgga ccattgcaaa
     3241 ggcttaccac gccttggaac aagaagaagt tgccttggct ctttataatg agatttcggc
     3301 tgatttggca gaaaatccag agtttttaca ggattatgcc tatttattaa gggagtttgg
     3361 ccagtttcac aaggctatcc aaatggctac cgcttacctc agacaagtac ctgatgatgt
     3421 gaatatgcaa gactttcttg accatatcaa gcatcaacaa tcagaataaa aaaacagttc
     3481 tcctagggga aataactttc ctaggagagc tgttttttag ttgcttatca gaataatttc
     3541 atagagagaa ttttggcttg gtcaggagta acccgtaagg ttttttgacc agtataagtc
     3601 acaaatcctg gcttggcgcc actaggtttg tgtaattttt tagcttcaat catatcgacc
     3661 tgaacgagat ttgataacct agctttcgaa taataagcag ctagttcggc agcatcagtt
     3721 ttaacctcgt cacttgggtc aagattgtct ttgatgatga catgacttcc agggatatcc
     3781 ttagcatgga accagagttc tcctttttta gccattttaa aggtgagttc ttcattttgc
     3841 aagttattgc gtcccaccat gagaatggtt tttccatctg aagccaggta ctgctcaggc
     3901 tttttacgtt tatggcgttt gtctctttgg cgacttttca aaaagccagc ctgatataat
     3961 tcttcgcgaa tatcttcgat gtcgtcgata cttgcttgag ataaattgta gtccacgctc
     4021 tcaaagtaag taatactttg cttggtatcg gcaatcagtc cagataagtg tttgacagct
     4081 tcttttagct tttggtattt tttaaaataa cgttgagcat tttgatttgg tgtcagagcc
     4141 ttgtctaagg caatctcaat tttttcccct gtataataat tgtctaaaat cacagagtct
     4201 tggttgtttg ggaccagact gaggtaggtg gtcaataatt ctcctttttg acggaataat
     4261 tcagcatttt ccgtagccaa aagttcagct tcttgtttac ttagtttgtt gcgatttttg
     4321 tccagttcag tttgcacacg gtgaatcaaa tcactggctt gctgattgat acggtcgcgc
     4381 tcagccttgt cttgataaaa gtggtcaagc atgtcagaca aagtctcaaa cgtcgcatgg
     4441 ctatctgaga aaagaacagg cgcaaaagaa gctgtggtca gatttgcctg agtaggtcta
     4501 gcaaaaaact cacggaaccg ttttaatttg tctgtggtca gaagctcggc caactctttt
     4561 gcggtgtctc gccctaagcc ttgaaaatgc tgttgcaatc ttttaactgt tagttcttgt
     4621 gtttggagga tttcaaatag aggaacatct gtaattgtaa aaggattaac agcagctgtt
     4681 ttagggggct caatgtaggt agagccaggt aaaatggtgc ggtaagagtt ttgtgaaaag
     4741 ccaacatgtt taatggattc gatgatttta ttttctgcac ggtccaccaa gatgatattg
     4801 ctgtgcttcc ccatgatttc aatgattagg gttgcctgaa tagcatctcc gatttcattt
     4861 ttattagata ccttgatttc gataatgcgg tcattatcaa tctgttctaa ctgctcaatg
     4921 actgcaccct ggaggtattt acgcataatc atggtaaagg tattaggtac ctgaggattt
     4981 tgaaagtctg cttgggtaat ttgaagtcgt ccaaaaacag gatgggcaga caatagcaat
     5041 ttataattct tgcgatggtt acgaatggtt aagacaagtt cccgttcaaa gggttgattg
     5101 accttttgga tacgaccgta aagcaagttc tcttttaatt cgttggttaa atggtgtaag
     5161 aaaaagccgt caaaagacat ggtttctcct ttctgaattc taatctcttc attataccac
     5221 aaatacttga ctaaaaatat ggacgaaaga gcttgcaatt tttttgattt aggttataat
     5281 gttaaaaaaa cgagaaagga agtcaatgat ggtaaaaaca caacacttag caaattggca
     5341 aagctggcat agataagttg tgtttatgaa ttggcataga tacgactttt agagtacttc
     5401 taaaaagata tctatgcgct gacattggat ttcacaagag cgcctagtaa tcattactaa
     5461 gtgctttttc ttttgagaag tattctcatc atcaacgttt taaagagatt gacatgaaat
     5521 gtgaggaatt cagatgaaaa acaagagttt aatagctacc ctccttgttt taactgtaat
     5581 agtgataggc tctctattat caaaaggagt atcaaaggaa aaccgtgatt tggctaacca
     5641 acaaaacatt acaattggca tcttacaatt ggttactcat gaggctcttg atgatattaa
     5701 gcgaggtatt gaagatcaac tcaaaaagca gaagcctcaa aaacaaaatg tagtgattaa
     5761 ggttatgaat gctgaggggg atcagagtaa aatccaaaca atgagtaggc aattagtgca
     5821 atcaggaagt gatatcgtga ttggtatcgc aaccccggcg gcgcaaggct tagcagctgc
     5881 cagcaaggat atcccagttg tcatgtcagc agtatctgat ccagtaggat ctagattagt
     5941 gatgcaattg gatcaacctg aagctaatgt aactggtcta tctaataaag tgcctgttaa
     6001 acagacaatt gatttaatga aaaagctgac accacatgtc aaaacagttg gaattttata
     6061 tgcaagtaat gaggataatt ccttatctca agttaaagag tttaggcggt tagctcgcaa
     6121 aaagggctat caagttattt catacgcggt tccttctact aacgaagttc ctgccacgat
     6181 gtcagttatg ttaggtaaag tagacgcggt atttatcccc caagacaata ctatagcaag
     6241 tgcattttca tcagtcatga caaccagtaa ggctgcaaag attcctgtct acactagcgt
     6301 tgatcgaatg gttgaaaaag gtggtttggc agctatttct caaaatcagt acgatttggg
     6361 agttcaaaca gctaatcagg tgttaaaatt aatcaaagga aagcgtgttg tagatgttcc
     6421 tgtcaaagtt gtggatattg gtcaaccgct aattaataag aatgtagcag cagaattagg
     6481 tattgctatt aacagggaag attttccttc agcttctttt attgagaatt aatacaaaga
     6541 catgtgatat aaaaggtcaa gaaaaaacca taaaaaacac cctcactgag gtgaatttgg
     6601 atgccaagtc agacaatcgt cttgaggatg taatggttat ttttatcatt tttagtttta
     6661 aaatgctcta ttttagacac gtaaaaaatc ttgccttttg agtaagttta aacatgattc
     6721 tcgacttttt tgttttctgt cttagtttcg tcaaagtgat tgaaaaaaaa gactaagcca
     6781 cggtgctgaa ctaagtataa aacaaatcta aaaattaatg taaagaaagg gtttatactg
     6841 gcgagcgtta cccgctaata taaatatgag gaaaaatatg attatttcat cagtttcaca
     6901 aggccttata tggggagttt taggattagg aatctacctg acctttagga ttttaaactt
     6961 ccctgacatg actacagaag gttcttttcc tttaggagga gctgttgcgg tcaccgctat
     7021 tagcttaggg tggaatccat ttctatcaac cttacttggt atgttatcag gtgctttagc
     7081 aggttttcta acagggctgt tatatacgaa aggaaaaatg ccaacacttt tagctggaat
     7141 tttggtgatg acgtcatgta actccattat gctaatggta atgggaagag caaacttggg
     7201 tttacatgat cataaacgta ttcaggattg tttccctttt tcaattgatt taaatagcct
     7261 tttaacaggt ctgataactg ttgtgattgt tattagcgtt ttgatctatt tcctttacac
     7321 taatctaggt caagcttata ttgctacggg agacaataaa gatatggcta agagttttgg
     7381 tattaacacg gattggatgg aagttatggg acttgtggtt tctaatagtt taatagcttt
     7441 atcaggtgct ctagtgagtc aacaagatgg ctatgcagat gtctcaaaag ggattggagt
     7501 tattgttatt ggtttggcga gcataattgt tggtgaagta ctatactcaa ctggactgac
     7561 tcttttagaa cgtttgattg ctattgtaat aggctcgatt ctttatcaat ttttaatttc
     7621 agtagttatt acgctaggtt ttaatacaag ttatttgaaa ttaattagtg ctcttgtttt
     7681 agcactgtgc ctcatgatac cagtggtaaa agagcgcttc tttaagggag tgaggttaac
     7741 acgatgacaa aaattattga attgatcaat gcaacagttg acgtagataa tggttttgaa
     7801 gatgctaaaa caatattgga caatgtgaca ctaactatct atgaacatga ttttttaact
     7861 attttaggag gaaatggagc tggaaaatca acccttttta atgtaattgc aggaacgcta
     7921 agtttgacac gggggcaaat taggattctt ggtcaggatg tgacgcattg gccggcagaa
     7981 aaaagagctc tttacctatc gcgagtcttt caagatccca aaatgggaac agcccctcgg
     8041 atgacagtgg ctgaaaatct cttaatcgca cgtcaaagag gagaaaaacg ctccttggct
     8101 tctcgaaaaa taacagagca cttagctagt tttgaggatt tggttaaacg aacaggaaat
     8161 ggcctagaaa agcatttaga aacaccagca ggtcttttgt ctggtggaca aagacaggct
     8221 ttaagtttat taatggcaac gttgaaaaag ccagctctct tattattaga tgagcatacg
     8281 gctgcccttg accctaagac gagtcaatct ttaatgcaac tgacagatga gtttgtgaca
     8341 aaagatggct taacagcttt gatgattacc caccatatgg aagatgcatt aacttatggg
     8401 aatcgtctga ttgtaatgaa agatggaaac attattaaag atttaaatca aatggaaaaa
     8461 gaacaactga cgattactga ctattaccaa ctatttgatt agtcaacgat ctaaatcatt
     8521 gactaataat aagtcagtga ttttttgatt tctgtactga aaataatctg aaagtatcaa
     8581 ggctatttag agaaaaaacg cctcaaatat gatatactat agaagatagt agcatagcta
     8641 cataacagta attcgactta cgttgtaagg accgtttgta ataataaaat atttaaagga
     8701 gtatcatgac tgatattaaa atgattgcct tgggaggggt ccgtgaatac ggaaaaaact
     8761 tctatttggt agaaattaac gattctatgt ttattttaga cgcagggctc aagtaccccg
     8821 aaaatgagca acttggggta gatttagtca tccccaattt agattatgta attgaaaaca
     8881 aaggtaaagt acaagggatt ttcttgagtc atggccatgc tgacgccatt ggagcccttc
     8941 cttatttgtt agctgaagta tcagctcctg tatttggatc tgaattgact attgaattag
     9001 ctaagctatt tgtgaaaagt aataacagca ctaagaaatt taataatttt catgtggtag
     9061 acagtgatac tgaaattgaa tttaaagatg ggctagtttc gttctttaga acaacgcatt
     9121 caatcccaga aagtatgggt attgttattg gaacagataa aggaaatatt gtttataccg
     9181 gtgacttcaa atttgatcaa gcagcaagag aaggttatca gacggatttg ttacgtttag
     9241 ctgagattgg gaaagaaggg gttctagctc ttttatcaga ttcagtcaat gcgacaagca
     9301 acgatcagat tgccagcgaa tctgaagttg gtgaagaaat ggacagtgtt atctctgatg
     9361 ctgatggccg tgtcattgta gctgcagtag cctcaaatct tgttcggatt cagcaagtat
     9421 ttgattccgc cacagcccat gggcgtcgtg ttgttttaac cggaacagat gcagaaaaca
     9481 ttgtcagaac agctttacgt ctagaaaaat taatgattac ggatgagcgt cttttaatta
     9541 aacctaagga tatgtctaaa ttcgaagacc atgaattaat catccttgaa gctggtcgta
     9601 tgggtgagcc aattaatagc ttacaaaaaa tggcagcggg acgtcaccgt tatgttcaaa
     9661 tcaaagaagg agaccttgtt tatattgtaa caacaccaag tacggctaaa gaagctatgg
     9721 tggcgcgtgt tgaaaacttg atttacaagg ccggtggttc tgttaaatta atcactcaaa
     9781 acttacgcgt gtcaggccat gctaatgggc gtgatttgca actattgatg aatttgttaa
     9841 aaccccagta tctttttcca gtacaaggag aatatcgtga tttagctgcc catgcaaagc
     9901 tagctgaaga agttggtatt tttccagaga atattcatat tctgaagcgt ggcgatatca
     9961 tggtgcttaa tgacgaaggt tttctccatg aaggaggtgt acctgccagt gatgtcatga
    10021 ttgacggtaa tgctatcggt gatgtgggta atatcgtctt acgagaccgt aaagtcttgt
    10081 cagaagatgg tattttcatt gttgctatta ctgtttctaa aaaagaaaaa cgcattattt
    10141 ctaaggctaa agttaatacg cgtggatttg tgtatgtcaa gaaaagtcat gatattttaa
    10201 gagaaagtgc agaattagtt aatacaactg ttggcaatta cttaaaaaaa gatacctttg
    10261 actggggcga attaaaaggg aatgtcagag atgatttgtc taaattctta tttgaacaaa
    10321 ccaaaaggcg cccagcgatt ctgccggttg tgatggaagt tcgttagtga aaataggaag
    10381 gttaggcaaa aactgcctac cttctttgat tttaacaact ggtaaaccag gaaactgttc
    10441 acccaaaaat acagtagatt tacgtgatag gattattttg gtataaagga gtgtaatgaa
    10501 tggcaaatat tgcaattgaa taccattcgg tagttttagg aatggagcgt aaggtaaatg
    10561 tgatttatcc tgaccagtct gaaatcccta aaaaagatca aggggataag gatattcctg
    10621 ttttatattt gttacatggt atgggaggca atgaaaactc ttggcaaaaa cgagcagcta
    10681 ttgaacgctt gttgcggcat accaatttga tagtcgttat gccttcgaca gacctaggtt
    10741 ggtatacaga cacggcttat ggacttaact attatcgggc gttgtcacaa gagttaccgc
    10801 aggtcttagc agctttcttt cctaatatga cccagaaacg agaaaaaacc tttgttgctg
    10861 ggctctctat gggtggctat ggtgccttta agtgggcact caaaagcaat cggttttctt
    10921 atgcggcatc gttttcaggc gctttggatt tttctcctga gaccttgctt gagggaaacc
    10981 tgggagaatt agcctattgg cagggcgttt ttggccagtt tgatgatcct gatcttgata
    11041 agcattacct taaaaatatg gtggcagaaa gtgatggaaa aaccaaattt tatgcttggt
    11101 gtggctatga ggattttctt tttgccacta atgaaaaagc cattgctgat tttcaggctc
    11161 aaggtcttga cattgattat cataaaggtc atggtaaaca tgagtggtac tattggaacc
    11221 agcaattaga agtcctgtta gagtggttac cgataaacta ccaaaaagaa gaaaggttgt
    11281 cttaaccatt tcctagtaca acatgaggtg acaaatgaaa caattactgg cgatcatatt
    11341 gtggctgcct aagctaatag tgaaaatgtt ttggcatctt atcaaagggt ttttacaaac
    11401 tatcttatta gtaacgatta ttattatagg gctgatgtac tattctaatc acagtgattc
    11461 cgttttagcc aataagatct cgatagttac tgagcaagtg gtgcaaattt ttgatatctt
    11521 aacacaaaag ccatctgcaa agacgcgtca tgggagtggt aatagtcatt ccactttcct
    11581 tgttagcgct aaagaaggta atcaacgact taaaaagagt tcgatttctt ttggaaacta
    11641 ggctgaaaat gtcctcaaaa gggcattttt ttgttagaat gaaagtatga ttattttaca
    11701 aggaaacaag ttagaacgct ctttctcagg agatgttctt tttcaaaaca tatcactgca
    11761 agttgatgaa agagacagaa ttgcattagt ggggccaaat ggagctggta agtcaacctt
    11821 actcaagcta ttggttgggg aagaaacccc aacgagcgga gaagttaata ctaaaaaaga
    11881 cctaaccttg tcttatctgg ctcaaaatag ccgttttgaa tcagatcaaa caatatatga
    11941 ggaaatgctc aaggttttcg aagcattacg ccaagatgaa aagcgcctgc gtcagatgga
    12001 aatggatatg gcaaccgttt caggtcaaga tttaacccga ctgatgacag attatgatct
    12061 gcttacagag cattttcgtc agcaaggcgg ctttacgtat gaatctgaca tcaaagccat
    12121 tttaaatggg tttaagtttg atgagagcat gtggcagatg actatcgctg agctgtcagg
    12181 gggtcaaaac actcgtctgg ccttggctaa aatgctttta gaaaagccag aactattggt
    12241 ccttgatgag ccaacaaacc acttagatat tgaaaccatt gcctggttag agaactactt
    12301 ggccaattat caaggagctc taatcattgt gagtcacgac cgttattttt tggataaggt
    12361 ggcaacagtg accttggatt taactcctca tggcttggat cgctacgttg gcaattattc
    12421 tcgttttctg accttaaaag ctgaaaaatt agtagccgaa gaaaagcagt ttgacaaaca
    12481 gcaaaaagaa attgccaagt tagaagattt tgttcaaaaa aatattgtca gggcttcaac
    12541 tacaaaacgt gctcaggcga gacgtaagca attggaaaaa atagaacgct tagacaaacc
    12601 gactagtgct agaaaatcag ctcacatgac ttttcaggca gataagccgt ctggcaatgt
    12661 ggtgttgacg gttgaaaaag ctgctattgg ttataaccaa catgtcttat ctgaacctat
    12721 taacctagac gttcacaaat tagatgcgat tgctattgtt ggtccaaatg gtattggtaa
    12781 atccactctg attaaatctg ttattggaca gattcctttc atcaaaggag aagtgaaata
    12841 cggtgccaat gttgaaacag gttactatga ccaaacccaa tcacatttga cgtctagcaa
    12901 cacagttttg gaagaattat ggcaggattt ttcaaccacg ccagaagttg atatcagaaa
    12961 ccgattagga gcctttctct tttctggaga tgatgtcaaa aaatcggtgg ccatgctgtc
    13021 aggtggagaa aaagcccgtc tgcttcttgc gaagttatct atggaaaaca ataattttct
    13081 tgttctggac gagccgacta accacctaga cattgacagt aaagaagtgc tagagaatgc
    13141 cctcattgac tttgacggca ccttgctttt tgtcagccac gaccgctatt tcattaatcg
    13201 tttggcaact aaggttctgg aaattaccga aaatggctca accctttact taggtgacta
    13261 tgattattac cttgagaaaa aggcagagct agaagaacta gcccgcctag cagcaggaga
    13321 gacagttgag gaaacaaaag aagcctctgc cactgattat cagctacaaa aagctaatca
    13381 aaaagagcgt cgtcgcttga cccgccgata tgaggagatt gaagcaagac ttgaaaccat
    13441 tgaagagcgc attggtgcta ttcaagaaga catgcatgcc tccaatgata cggcgcagtt
    13501 aatcgcttgg caaaaggaat gggaccaatt ggatcaagaa caagaaactc ttatggaaga
    13561 atgggaaacc atcgctgaac aaatagaatc ataaaaataa gctttagcag ttttgctaag
    13621 cttattttta tcgctttgct acttgtttga aggtgtgaaa gaggttttaa aatgatatgt
    13681 tttttatgtc aaggtcggta aaacgtgaaa ctgttatata gtatttttaa attttttagt
    13741 acagttttgt ttgtaatttc acaaactatt gcatttctaa aaaaccaaat aaggtaaggt
    13801 agaaagcgat ttaattattt gcaaattgtg aaaaaaagtt ttatcataaa aaggtaatat
    13861 gataatatgg aggccgaaat ggtaacagtt tctaaagaac aacatttgga tatgttcctt
    13921 aaaatggaac gtattcgtga atttgattca cgtattaata aactcgtccg tcgtggattt
    13981 gtacaaggga tgactcactt ctcagttggt gaagaagcag ccaatgttgg agcggtggct
    14041 cacttgtctt acgatgacat tattttttca aatcaccgtg gacatggaca atcaatcgct
    14101 aaagacatgg accttaataa aatgatggct gagcttgctg gtaaagcaac tggtgtgtct
    14161 aaagggcgtg gtggctcaat gcacttggct gattttgaaa aaggaaacta tggtaccaat
    14221 ggtatcgttg gtggtggtta cgctcttgca gttggtgctg ccctcactca acaatacaaa
    14281 ggaactaaca atatagctgt tgctttctca ggtgatggcg ctactaacga aggatcattc
    14341 cacgagtctg ttaatatggc tgcaacatgg aaacttcctg ttattttctt catcatcaac
    14401 aaccgttatg gaatttcaat gagcatcaat aatgcaacaa atacaccaca cttatacaca
    14461 cgtgcagaag cttacggaat tcctggtttc tactgtgaag atggcaatga cgtgatggct
    14521 gtttatgaaa caatggggaa agccgttgaa catgttcgtg gtggaaatgg tccagctatt
    14581 gttgaggtag aatcataccg ttggtttggt cactcgactg cagatgctgg taaataccgt
    14641 actaaagaag aagtggatga gtggaaagaa aaagatccaa tgatcaaata tcgtacttat
    14701 ctgacaagcg aaggcattgc cactgatgac gaattggatg ctattcaagc acaagtcaaa
    14761 aaagaagttg acgatgctta tgaatttgca caaaacagtc cagatccaga actttcagta
    14821 gccttcgaag acgtttgggt tgactaagat caccttgtca gtaagaagca atcgtatttg
    14881 aataataaat ggagaagtaa aaatgatgtc agaaacaaaa ttaatggctt tgcgtgaagc
    14941 agtcaacctt gctatgactg aggaaatgcg caaggacgaa aacattttcc ttatgggaga
    15001 agacgtaggg gtttacggag gagacttcgg gacttctgtt ggaatgattg aagaatttgg
    15061 tcctaaacgt gttaaagata ccccaatttc agaagctgct atctctggtg cagctattgg
    15121 tgcagcgatt acaggactac gtccaattgt cgacgttacc ttcatggatt tccttaccat
    15181 catgatggac gctatcgtga ataatggtgc taaaaataat tacatgttcg gtggaggcct
    15241 tattacccct gtaaccttcc gtgtggcatc aggttctggt attggttccg ctgcgcaaca
    15301 ctcacaatct cttgaagcat ggttgactca cattccagga atcaaagttg ttgctcctgg
    15361 taatgccaac gatgctaaag gcttattaaa atcagctatc cgtgataata acattgttct
    15421 tttcatggaa ccaaaagcgc tttacggtaa aaaagaagaa gttaaccaag atcctgactt
    15481 ctatatccca cttggcaaag gtgacatcaa acgtgaagga actgatttga caattgtgtc
    15541 atacggtcgt atgttggagc gtgttcttca agcggctgaa gaagttgcag cggatggtat
    15601 taacgttgaa gttgttgacc cacgtaccct cattccactt gataaagagt tgattattga
    15661 atctgttaag aaaacaggta aattgatgct tgtcaacgat gcctacaaga ccggtggatt
    15721 cattggggaa attgcaacca tgattaccga aagtgaagcc tttgattacc ttgatcatcc
    15781 aatcgttcgt cttgcaagtg aagatgttcc agtaccttat gcacgtgtac ttgaacaagc
    15841 gatcttacca gacgttgaga aaatcaaagc tgccattgtg aaaatggcaa acaaaggcaa
    15901 ctagaagata aggcaagtgg tctgataaag gctatgttta tcagtaacgt ctcagctaaa
    15961 aactatttta aaagttttta gaagaactga gttgtcttat cccgtgtcgc ggcacctcac
    16021 ctttcgtatg ttttcgtaac aaggtgggga tgttgatggc gcttatcaaa tcgtgaaagg
    16081 atgtagaaat ggcatttgaa attattatgc caaagctggg cgttgacatg caagaaggcg
    16141 agatcatcga gtggaaaaaa caagaaggtg ataccgtcaa tgaaggtgat atccttcttg
    16201 aaatcatgtc agacaaaacc aatatggaac ttgaggcaga agactcaggt gtgctattga
    16261 aaattgtccg tccagctggt gatactgtac ttgtaactga agttatcggt tacattggtg
    16321 cagagggtga atcagtagat actattgctt caagtgaaaa aacaacagaa attccagttc
    16381 ctgcttcagc agatgctgga ccagcagttg ctccaaaaga aaatgtggca agtccagctc
    16441 ctcaagtggc agcgacagct attccgcaag gaaatggtgg aaaagttcgt gctactccag
    16501 ctgcacgtaa ggtagcagca gaaatgggca ttgaccttgg ccaagttcca ggaacaggac
    16561 caaaaggacg tgtccataaa gaagacgttg aaaactttaa aggtgctcaa ccgaaagctt
    16621 caccacttgc tcgtaaaatt gcggcagaca aaggtattga tttggcaacc gtatcaggta
    16681 ctggctttaa cggtaaggtc atgaaagaag acatcatggc tattcttgaa gcggctaaac
    16741 cagctgaagc aaaagcccca gcagctaaag aagaaaaagt tgttgacctt ccagaaggtg
    16801 tggaatacaa gccaatgtca gctatgcgta aggcaatctc taaaggtatg acaaactctt
    16861 accttactgc ccctaccttt acgcttaact acgatattga catgacagag atgattgccc
    16921 ttcgtaagaa gttaattgac ccaatcatgg ctaaaacagg ccttaaagtc agctttacag
    16981 acttgattgg tatggcagtg gttaaaacct tgatgaaact tgagcatgag tacatgaatg
    17041 cttccctcat caatgatgct aatgatattg aattgcaccg ctttgtgaac cttggtattg
    17101 ccgtaggtct tgatgatggt cttatcgttc ctgttatcca tggggctgac aagatgagct
    17161 tgtcagattt tgtgcttgct tcaaaagatg tgattaaaaa agcgcaaact ggtaagttga
    17221 aggcagctga aatgtctgga tcaaccttct ctatcacaaa cttgggaatg tttggtacga
    17281 agacatttaa cccaattatt aaccaaccaa actcagcaat tcttggtgtt ggagcaacta
    17341 tcccaactcc aacggttgtg gatggtgaga ttgttgcccg tccaattatg gcgatgtgcc
    17401 taaccatcga ccaccgttta gttgatggta tgaacggtgc aaaattcatg gttgatctca
    17461 agaaattgat ggaaaatcca tttgaattgt taatctgatc aatcagtttt aaacaagaaa
    17521 agcattcaat aataaaaggg attttgctaa gtttagactt ggtgggtgcc cttttctcaa
    17581 aaatatagaa aggatgtaga acgaggagat ctcatgcaaa tgagtgataa gcaattgaat
    17641 ccgagcgaaa agctcggata aaaatggtaa gagttggcta atcttagcgt cgtagacgtc
    17701 tgaactttcc tcaatttttg tttcggaaag tcctagtcgt cctgacgtgg gtgcgtcaac
    17761 gaaatcaaag atttctgact taccgcaaaa attcaatcgc tttctccttg ctcttggtat
    17821 cttaaattat ggctgttgaa attattatgc caaaactcgg tgttgacatg caagaaggtg
    17881 aaatcatcga gtggaaaaaa caagaaggtg atactgtcaa tgaaggcgat attcttcttg
    17941 aaatcatgtc agataaaacc aatatggaac ttgaagcaga agactcaggt gttcttctta
    18001 aaattacacg tcaagcaggt gaaacagtac ctgtaacaga agttatcgga tatatcggtg
    18061 ctgaaggtga atctgttgag gtttctagcc cagctgcttc agatgtgaat gttgctcgta
    18121 caacagaaga tttagaagct gctggacttg aagtgccaaa agcaccagct caagctgctt
    18181 cagctgcacc aaaagctgca cttgctgatg atgagtatga catcatcgtt gttggtggtg
    18241 gccctgctgg ttattatgca gctattcgtg gtgctcaact tggtggtaaa attgccatcg
    18301 ttgagaaatc tgaatttggt ggaacttgct tgaacgtagg gtgtatccca accaaaactt
    18361 accttaaaaa tgctgaaatc cttgatggta ttaagattgc agcaggacgt gggattaacc
    18421 ttgcctcaac taactatacc attgacatgg acaaaacagt tgactttaaa aacaccgttg
    18481 ttaaaacctt aacaggcggc gttcaaggtc ttctaaaagc taataaagtc actatcttta
    18541 atgggcttgg tcaagttaac cctgacaaga ctgtaaccat tgggtcacaa accattaagg
    18601 gtcgcaacgt tatccttgct acaggttcta aagtatcacg tatcaatatc ccaggaattg
    18661 actctaaact tgtcttaacg tctgacgata tccttgacct ccgtgaaatg ccaaaatcac
    18721 tagcagttat gggcggtggt gttgttggta tcgagcttgg acttgtttgg gcatcttacg
    18781 gtgtggatgt taccgttatt gaaatggctg accgcattat cccagctatg gataaggaag
    18841 tctctcttga acttcaaaaa atcctttcta agaaaggcat gaagatcaaa acatctgttg
    18901 gtgtctctga aattgttgaa gcaaataacc aattgacctt gaaacttaac aatggtgaag
    18961 aagttgtcgc tgaaaaagct cttctttcta tcggtcgtgt accacaaatg aacggtctgg
    19021 aaaatcttaa ccttgaaatg gatcgtaacc gtatcaaagt taatgactac caagagacat
    19081 caattccagg tatctatgcg ccaggtgacg ttaacggaac gaaaatgctt gctcacgctg
    19141 cttaccgtat gggtgaagtg gctgcagaaa atgcgataca tggcaacaca actcgtaaag
    19201 ctaaccttaa atacactcca gcagccgttt acacacaccc tgaagtggca atggttggtt
    19261 taactgaaga acaagcgcgt gaacaatatg gtgatgtgct tattggtaaa aacagcttta
    19321 ctggtaatgg acgtgcgatt gcttcaaatg aagcccatgg ttttgttaaa gttattgctg
    19381 atgccaaata ccacgaaatc ttaggtgttc atattattgg tccagctgca gctgagatga
    19441 ttaacgaagc agctactatt atggaatctg aattaaccgt tgatgaattg ttattatcaa
    19501 ttcatggaca cccaaccttc tctgaagtga tgtacgaagc ctttgcagat gtgcttggcg
    19561 aagctatcca taacccacca aaacgtaagt aaaacgtctt tgtcttatta actaatgaaa
    19621 aatatcatca cataacagat cacaaaaaag actagcacaa ctatgaactg caccccaaac
    19681 gttagatata aaaccctaac gtttggggtg tttttgtatg acattaagct atgaagataa
    19741 gctagacatt tagcgctaaa aaaacgagag aactccgttt gaaagaagca tctctgaaaa
    19801 gaagaagaca aaaaaccatt ataaagaatg gacaaagacg agctctgatt agattttctt
    19861 ttaaggacgg ctaaagtgac tcgttctacc tactattatc atcgattatt gcaccaataa
    19921 ccgtatcaaa ataaaacgaa aaggacttag tcctctacaa tacagaacta aatcctttac
    19981 ttcaaataga gtagccaact tttggggctc agtgcagttt ccagtttttt gtctttaata
    20041 acgtttcatt gttaaccctt actttccaat aacagctaaa tccttaagtc tttcttacga
    20101 aagggttttg tgatatggtt aaaagaagca actaaagctc cacctaagaa ttctaaagag
    20161 gagaactgat acccagtatg cggtaagcgt cgttgtcttt tgtgggtaat tttatgagag
    20221 ttaagagcat tgtttttagc tgcaattcct tgatgagcaa gcttttattg ctgattttgc
    20281 ttgacaggat gaggaaaact ataaaagcct tgatgagaaa gattatgatt tgaaaacatc
    20341 gttgcttttt ttccatagtg aatgacgtgc atctgttgac tatcatgatc gtagacagcg
    20401 gccagtttgt cattgttttc taacaagtcg atatcaagct tgctaatata ggtttcaaat
    20461 tcttgacgag tcatatttgg aagcatcata taggcataac gatcgccatc ttgagtatgg
    20521 ttttgcatga tagtgataaa ggtatttgaa acttctttga tggcttctgg tgatttgtca
    20581 tcagctttta tgttttgcca ttgccctgtt tgaagtgcct tacttatgct aagtgttgtt
    20641 ggcttgaaga agtagtaacc aatattttga gcgggatcat cactttcaag gaaaatactt
    20701 ttagtgtttg taaaatcaac tagctgatta ttcaagtcaa cgggttgatt gttaacataa
    20761 gcatggtaag ggtgcttttg attttctttt cgttgttcaa tagttgtata cgccttgtga
    20821 gatgactggt ttttgatatt gctaccaaca aagattattt tgtttcctaa gataaaccac
    20881 cctttattga gagtgaggct tttattccaa ttagtgaagg tcatagcagc tagagcactt
    20941 gtgttattaa gttttttact tgcaacaaaa gcgtcatcag agagactagt catgccaact
    21001 tgttgatagt tcgttttaat attctcagga gttccctcta gtggtttttg ctcagtttct
    21061 gtggttccag gtaagcggta gggatttacc gttgcccaat agttttcact gtagtgtcct
    21121 aaatcgttat tgtataggta aaacattcca tcagaagtaa accagccatg aagattttca
    21181 ttattcatag cttcataatt ttgagttcga ttcgaaaaca ttgataggcc aaaagcaaaa
    21241 tcgtgtttat tattatatag tgccaattta tccatactat tgaaactagc tacgtaacta
    21301 tcaagttttt ggactggaac aaaagtatca tttagtagtt ctttcataag tttgatatcg
    21361 tgataggttt tcaaattatc atagacattg taaaaagcat tcccttgtgt gacgagtgtt
    21421 tttatacgtg ttttaagtgc caaacggtga ggctcttcag acatgtcagc aatacgtaaa
    21481 atagcacgaa gtgcttcaat gccagcaaca tgagattgag cattaaaacg actgatagaa
    21541 cgccctcgag tcatatccat catttctcca cgaacgatga tagggaaaaa agaatggtta
    21601 atccaatgat agatagtagc cattttatcc gcttctatag gagactttgt tttttgaata
    21661 ataggaatta attgcgataa gccatctata agcacattac cgtaagctcc agtgtaagca
    21721 atgccttttt tataaagtgg actttgagcg ttagtaacca cgtgatcaat taaagaaccg
    21781 tcttggtaaa aaccatttcc ttcgtcaact agcgtgaaaa ctttctcaat tgctttgatt
    21841 gtatcactaa tttcgagatc atctttacga agaataccgg aaatgagttt aacacgcccc
    21901 atatcaatta aatttccgct attggcttca aaaggtgaaa aattggcggc gcgaacccta
    21961 aaacgagtag ggtcaggcac aaatttttcg attggagctg tgtatttaag aatttcttct
    22021 tgagtaaaat aaggatacat caaggataag gtattattaa tagcacgagg agttccaatt
    22081 tcataaaccc accaattttc tttgttttct tttccagttg tttgatgatt ttcacgatct
    22141 agattataag cgtgttcata catgaaggcc ataccgtctt ttacaatagc aatagcttta
    22201 ctatcttgat aatagcatga ttcaggatta gtgatctgtt ttgctatttt ttcaatattg
    22261 cggtaagttt tcgtgatatt agtagaagcg gaataatcct ttgcatgttc ccaaaggtaa
    22321 gttctatttt cgtggtcagg cccttgatag cttttaataa tgttttgagc atccttttca
    22381 gctttattat gaaaagtgac catgtcagga ttggttttat cataagcatc gttgcctgcg
    22441 ataatactgt tccattggtc taacaattca gtatagtagt ctttttgttg tggctgtact
    22501 accttttttt cgctatctgt agtagtgagc gtttgcgttt gaaagtaagt attattaggt
    22561 tctgaatttg aagtcagtgt atcggcataa atggcagttc cttggcctat catagcaaag
    22621 ctaaggaata ggtttgaata aagtagtaat tgtttatggt gtgtgcaaaa ataagtattc
    22681 actgatacat tcctccaata taaaaatgag ataataaaag tagattggtt tactaagtaa
    22741 aaatatacca cgttaaagta tttttataaa cccttacata aataatggct gagtttttgt
    22801 gcactaataa gtgtaggtac tgaagatgtc atacagaata ggacacttgt cttttatatt
    22861 tgttttcatt atactagaga ggtagattac atttttgaaa ggattttttc tcctatgaaa
    22921 tacatcgtta acaaaagtca caatcctgcc tttaacatcg ctttggaagc ctatgctttt
    22981 agagaactag tggaagaaga cgaattgttt atcctttgga tcaatgagcc agctattatt
    23041 attggcaaac atcaaaacac cattcaagaa atcaataaag aatacattga tgaacatggt
    23101 attcatgttg tgcgacgttt atctggtggt ggtgcggttt atcatgattt gaataacttg
    23161 aactacacta tcatttcaaa taaaacagcg gaaggcgcat ttgatttcaa aaccttctca
    23221 caacctgtga ttgcgacttt agctgatttg ggtgtaacag ccaactttac aggccgtaat
    23281 gatattgaaa ttgatggcaa aaaaatctgt ggtaatgctc aagcttacta caaaggtcgc
    23341 atgatgcacc atggttgcct tttgtttgat gtggatatga ccgttcttgg cgatgctctt
    23401 aaggtaagta aggataagat tgagtctaaa ggagtgaagt ctgttcgtgc tcgcgtgacc
    23461 aatattttaa atgaattacc agaaaaaatt accgttgagg aattttcaga taaaatcttg
    23521 accaagatga aagaaacgta tccagacatg acagaatatg tcttgtcaga ggatgagttg
    23581 gctaagattg aacaatctgc caaagagcaa tttggtagtt gggattggac ctacggcaaa
    23641 gcgccagaat atacgattgg acgcaatgtt cgttaccctg caggtaaaat ttcaaccttt
    23701 gcaaatgttg aaaattctat cattaaaaac cttaaaatct atggtgattt ctttggaatc
    23761 aaagatgtgc aagacattga gaacttatta attggttgta aatatgagta ccgtgatgtt
    23821 tttgaacgtt taaaaacgat tgatacgacc cagtacttct cacgcatgac cgttgaagaa
    23881 gttgctaaag ctattgtttc ttagtggttt taatcactta agaagctctg tcagaagaaa
    23941 aaactataaa actcctaagc gaaatatcgt gtcctgctta ggagtttttg tttgatgaca
    24001 tgaaagaaga acttaaactt tattaaagga agctttgctt ttaaggtcgg catattcttc
    24061 agtgatttct tgtttcagta tatcatcata gctagatagt gaaatagctg atccatactt
    24121 ttttttaaga gctgttgtta ctaagcggta agcaagatta acattgcgtg ataagatagg
    24181 accgtggaaa taactaccat agacgttttt atagtgaaca ccttcagttt ggtcttcttt
    24241 attattaccg ttgccataaa ccactcgtcc aagtggcttt tcgtcaccag ataagaaggt
    24301 acgtccttga tgattttcaa agccataata ggtttcgtta aattcgtcat tatgaatttt
    24361 aatatctcca ataaagcgat tttggtgctg attaagggtg tagtgtccca taattccaag
    24421 tccatcaatt ttaaccccat tagcttggac atagtattgc cctagtaatt ggaagccacc
    24481 acagatggca agcaccactt tattattagc aatgtaatcc gctaaggcag cttttttgct
    24541 aggcaaatcc ttggcaacaa tgctttgttc ataatcttga ccaccaccaa agaaaacaat
    24601 atcgtaatcg tcttgttcaa aggtatcatt gatagacaca atatcaacag taaccctagc
    24661 tccaagtttt tcagcgacat acttgagcat taaaatattg ccgttatcac cataagtatt
    24721 cataagatta ccatagagat gggcgatagt gaggtcgtag atgtaatctt gattttcggg
    24781 agattttagt gaagtgtatg tcattagatc atctcctttt caacgacatg gcggtcagca
    24841 agtaagctac ggaattctaa cattgccgta taggttgcta aaatataggc atgcttagct
    24901 tcttgttttt caatggtttc tatgatttgc tcgagctttt cagcttgctt tattttagta
    24961 tcgtcaaatc ctgtgactct aagtcttcta gcaatttcag agtggcggac gccgccagca
    25021 ttaatttctg taatgggcat ttgggtgatg agttcaaaat tagcatccca aatccagctg
    25081 gtatcaatgc cgtcagcgta attagcattt aaaagcacag ataagctaaa tgggtaatca
    25141 gctagctgaa tcatttcaag cgcttgactg gctccaacag gattcttgat aaggataagg
    25201 gtgcatgatt tatcaccaat agtaaaagtt tcttgtcttc caaaaaccgc cttacttttg
    25261 ttaaagcccg ccttgatttt ctcgggagaa acaccgaaaa attcagcaac agagacggca
    25321 gcgagggcgt tgtaaatatt atataagcca ccgacattga ttttgtaatc ttggccatca
    25381 atgataaact cagaactgtg attggtaatc gtcgtgagtt cagttaatcg ataatccaac
    25441 tgtggtctct tgaagccaca atttaaacaa ataaagtctc ctagattcgc ataagtattg
    25501 agacgatact gaagaatatg ttcgcatttt ggacataaaa tcccttctgt gttgtaatga
    25561 gctagctgcg gagcatgctt agcagtatca aaaccataat actgaactgg attaactatg
    25621 tccttagaag agaaaatagg actatctcca ttggctagaa tagttgcttt tggagcattt
    25681 cgagcaccat ctacaatcat ctgataagta gtataaatct ctccgtagcg gtccatctga
    25741 tccctaaaaa tattagtata aacaaaaaga ctaggtttta agtaagtggt aattctagga
    25801 agactagctt cgtcaatttc taaaacagca atttgccttc cagatttccc ttttttagca
    25861 gctaaaaaag tagaagtaat tccagtaatc atattggcac cacttggatt tgtgattatc
    25921 tcgccaaatg cctctttcaa aataccgaca gtaagagcag ttgttaacgt tttaccatta
    25981 gtaccagtga cgaccacaat atcatagtct ttagacaaat cctttaaaat atctttatta
    26041 caagctagag ccaatcgacc aggataagta gatcctcgcc ctagttttgt taaaatggat
    26101 tgagcagctt tgccagcaat aatgccgagt aatgtcttca ttttcatgct tatattttat
    26161 cataagttta ttgattttag atagggaaaa atctttgatc atgatatgat ataatatagc
    26221 taataacttt tttgatatag aaatgagagt tgtatgaata atttatctag tatcgatatt
    26281 aaatttttat taagcttatt tgcggatcct tggctgctgg cagtgcatct tttagatatt
    26341 ctaattgttg cctatttgat ttatcgtttt attaaagcat tgactggaac taaaatcatg
    26401 tctttagttc aaggggtcat tttctttttg gttcttcgtt ttattgcaga gtggattggc
    26461 tttacgacaa tcacatactt aatgaaccaa gtcattactt atggtgtaat tgcaggagta
    26521 gtcattttta cccctgaaat tcgagcagga ctggagaaat ttggtcgttc tacgcaagtt
    26581 tttttacaaa aacagtacgt cagcagtgaa tctgctttgg tcgatgcttt gattaaatct
    26641 gtggcctata tgggtccacg caagataggt gcattgattg ctattgaaca gactcaaacc
    26701 ttacaagaat atattgcgac aggaattccg ctgaatgccg acatttcaag tcaactactc
    26761 atcaatattt ttattccgaa tacgccgctt catgatggag ctgttatcgt tggtcagaat
    26821 aagattgtgg cagcctgtgc ttacttgcct ttgtcagaat caaaggcaat ttccaaagaa
    26881 tttgggaccc gtcatagagc ggctattgga ttatctgaaa actctgatgc actaactatc
    26941 attgtttcag aagaaacagg agctatttca gtaactcgca aaggccaatt tttacacgat
    27001 ttaagcacag atgaatttga aacggtactt aggacctacc tgatgagtga ttcaaatgtt
    27061 agtttaccat ggtataagaa aattctagga gggaaatcta aatgaaacga tttttaaata
    27121 gtcgtccttg gttaggaatg gtatcagtct tttttgcgat tctccttttt ttaactgcag
    27181 catcgagcaa tcataataat tctagctcgc aaatttatag cccgattgaa acctatacgc
    27241 atagccttaa agatgtacca attgatatga agtatgatag tgacaaatat tttatcagtg
    27301 ggtattctta tggtgcggaa gtttatctaa cctccactaa tcgtatcaaa ttagattctg
    27361 aagtgaacaa tgatacacgt aattttaaga ttgtggcgga tctaacacat agttatccag
    27421 gtacagtaag tgtcaaccta agagtagaaa accttccttc tggggtgaca gctaccgtat
    27481 ctccagataa aatatcagtc acaattggca aaaaagaatc taaggtattt cctgttagag
    27541 gaagcgttga tgctaaacaa attgcaaatg gttatgaaat tagcaaaatt gaaacggggg
    27601 ttaataaggt tgaagtgacc agtgatgaat ctaccattgc tttaattgat cacgttgtgg
    27661 caaagttacc tgatgatcaa gtgttagata ggaattatag tagtcgagtg accttacaag
    27721 ctgtctcagc tgatggaact attttagcaa gtgcaattga tcctgcaaaa acaaatttat
    27781 cagttgctgt aaaaaaaata acgaaatcag tcccgattag agttgaggca gtggggatga
    27841 tggatgatag cttatcagat attcaataca aattgtcaaa acaaacagct gttatctcag
    27901 gtagtcgaga ggtcttagaa gatattgatg aaattattgc agaagttaac atttctgatg
    27961 tcactaaaaa tactagtaag accgtgagtt tgtcttctag tcaggtgtca attgagccgt
    28021 cagtagtgac cgttcagttg acaaccacca aaaaataatg gataataact aggaaaattg
    28081 aatggaggaa gccattaggc ttatataaat tatgggaaaa tattttggaa cagatggtgt
    28141 tcgtggtgaa gcaaatgttg agctgacacc tgagttagct tttaaactgg gccgattcgg
    28201 tggttatgtt ttaagtcaac atgagaccga aagacctaaa gtttttgtgg cgcgtgacac
    28261 ccgtatttca ggagaaatgt tggagtcagc cttgattgct ggtctactat ctgttggtat
    28321 tgaagtttat aaacttggtg ttttagcgac acctggtgtc tcttatttag ttagaacaga
    28381 aaaagcaagt gcaggtgtga tgatttctgc aagtcataac cctgcgcttg acaatggcat
    28441 caaattcttt gggaatgatg ggtttaagtt agctgatgac caagaattgg aaattgaagc
    28501 tctcttagat gctccggagg acacgcttcc tcgcccgagt gcagaaggac ttggaacctt
    28561 ggtagattat ccagaaggac ttcgtaaata tgaaaagttc ttggtaacaa caggtactga
    28621 cttaagcggt atgacagttg ccttagacac tgctaatgga gctgcttcag tgtcagcacg
    28681 tgatgtcttt ttagatttaa atgcagaaat tgctgtcatt ggcgaaaaac caaatggtct
    28741 taatattaat gacggtgttg gttcaacgca tccagaacaa cttcaagagt tagttaagga
    28801 aacaggagca gatcttggtt tagcctttga tggcgatagc gaccgtctga ttgccgtgga
    28861 cgaaactggt gagattgttg acggtgatcg tattatgttt atcattggta aatatttgtc
    28921 agaaaaaggg cttcttgctc acaataccat tgtcacaacg gtgatgtcta acctcggttt
    28981 tcataaagct ttagataaac aaggtattaa taaagcaatt acagcagttg gagaccgcta
    29041 tgtggttgaa gaaatgcgta gctcaggata caatctcggt ggtgaacaat caggtcatgt
    29101 cattatcatg gactacaata ccactggaga tggtcagttg acagctatcc aattagccaa
    29161 ggtgatgaag gaaaccggga aatcactttc ggaattagct gctgaagtaa ccatttaccc
    29221 tcaaaaactg gtgaatattc gtgtggaaaa cagcatgaaa gaccgtgcta tggaagttcc
    29281 agctattgcg aacatcattg caaaaatgga ggatgaaatg gctggtaatg gtcgtattct
    29341 cgtgagacca agtggtacag aacctcttct tcgtgtcatg gcagaggcgc caacagatgc
    29401 cgaagttgat tattatgtcg atactattgc tgatgtcgtc cgtacagaaa tcggatgcga
    29461 taactaacga gttgagtaca aacaagaaac cttatttcta aggcttctgt caacaattgg
    29521 aaagaggctt ggggcgttta gcccaagtct ctttttataa attgctaaag tagaagagaa
    29581 aggatatcgg atgacaaaac agcaaatcga aaaactagcg gacttactgc ctccttttgc
    29641 agccacactt tacttagata tgattcctga atttcaggaa ggttatgctt catatgttta
    29701 tcaaacacca aaacaagata ttcaaaagcg ccgcatcaag cagttaacta aaaattttga
    29761 acgattacta gataccccat ctttttatgt gatgcctgtg acaagaaaaa cagcagagca
    29821 attgctagca gataaaggcc tagcttatca tttgttatcg acagttgcag ctaatcaagt
    29881 gtttgcggct gctgttaacg aggatcagac gctttttcaa gtgcttcgaa atggagcaag
    29941 agtagccttt gtgattcttc aagaaaaggg tgaatttcta gaaatgactt tagggattct
    30001 tgaaacagtg acagaaaacg aggttccagc agctattgtg gaggctgtcc agacatatct
    30061 tctgacagat atgcaagaga aactcccttc tttaagcatt atggtcagtc aagaatcaag
    30121 caagcttttt taccaagaac aagggtttgt tttgctagct aagggttgca agccaggaaa
    30181 cgaaaatgac tctgacgttt gtcagctcgt aaaatacttt tccaagccat gaagaatcaa
    30241 gggaaatggt ttattcatag aaacatttta tcatcaaagt ggtataataa gaccatgtca
    30301 aaaaaaccta cttcagccta tgtgcacatt cctttttgca cacaaatttg ttattattgc
    30361 gatttttcaa aagtctttat ccaaaatcaa cctgttgatg cttatttgaa agccttgatt
    30421 caagaattcg aatcctatga cattcatgac ttaaagactc tttatattgg aggaggtacg
    30481 cctacagcta tcactgctaa acagttggag tatctcctaa atcaccttga acgaaacctc
    30541 aatttagatg atcttgaaga attcacaatt gaggcaaatc ctggtgattt gacgccagaa
    30601 aaaattgcgg tgcttcagag gtcagctgtc aatcggattt ccttgggggt gcagactttt
    30661 aataacaagc aattaaagca gattggccgt agccataatg aagaacaaat ttatagcacc
    30721 attgccaatt taaaaactgc aggctttcac aatatttcca ttgatttaat ttatgccttg
    30781 ccaggtcaaa ccttagaaca agtcaaggaa aatgttgcta aagctttggc acttgatatt
    30841 ccccatctta gtctttatag ccttattttg gaacatcaca ccgtttttat gaacaaaatg
    30901 cggcgtggta aacttaactt accaacggaa gacttggaag cagaaatgtt tgagtatatt
    30961 atttcagaaa tggaagctaa tggttttgag cattatgaaa tttccaattt taccaaacca
    31021 ggttttgaaa gtcgccataa cttgatgtat tgggataatg tggagtattt tggatgtggt
    31081 gcaggggctt caggttacct taatggcatt cgttatcaaa atcgggtgcc catccagcat
    31141 tatcttaaag cggttgaagc aggaaacgct agactaaatg aagaagtgct gcgcaaagag
    31201 gaaatgatgg aagaggaact ctttttgggc ttacgtaaaa agacaggagt ctccatccag
    31261 cggttccaag aaaaatttgg tatttctttt gaggagcgtt atggaaatat tgttagggag
    31321 cttcaaaacc aaggactgct tgtcaaagat gacgcctttg tcagaatgac taaaaagggt
    31381 cttttcttag gtgattctgt tgctgagcgg tttatattag attaggagag tattatggga
    31441 ttaagttatc aggaggagtt gacacttcct tttgaattat gtgatgtcaa atcagatata
    31501 aaattgcccc ttttattaga ctattgtttg atggtttctg gtagacagtc tgcgcaatta
    31561 ggacgaagta acaacaatct tttagttgat tacaagcttg tttggattgt aacggattat
    31621 gagatcacta ttcatcgctt gccacatttt caagaaacca tcaccattga aacaaaagcc
    31681 ctttcctata ataaattttt ttgttatcgc caattttata tttatgatca agaggggggt
    31741 cttttagtgg atatcttagc ctattttgct ttgttaaacc cagatacgcg aaaagtggca
    31801 actattccag aagatttagt agcgcctttt aagactgatt ttgttaaaaa gctacaccgt
    31861 gttcctaaaa tgcctctttt agaacaatca attgatcgtg attattatgt gcgttatttt
    31921 gatattgata tgaatggtca tgtcaacaac agtaaatatt tagattggat gtatgatgtg
    31981 ttggggtgtg cgtttttaaa aacgcatcag cctcttaaga tgactttgaa atatgttaaa
    32041 gaagtctcac caggcggtca aattacttcc agttaccatt tggaccaatt aacttcttac
    32101 catcaaatca tctcagatgg gcagctgaat gcccaagcca tgattgaatg gcgagcgatt
    32161 aaacaaacag aaagcgagat agactagtgc cttataaggg ttatttaatt gatttagacg
    32221 gaacgattta ccaagggaaa aaccgtattc cagcagggga gcggtttatc aaacgcctac
    32281 aagaaaaagg cattccctat ttattagtga ctaataatac cacaagaaca cccgaaatgg
    32341 tgcaaagcat gctgactaat cagtttcatg ttgagacagg cattgagacc atttatacgg
    32401 ctaccatggc aaccgtcgat tacatgaatg acatgaaccg tggcaagaca gcttacgtta
    32461 ttggagaaac agggctcaaa tcagctattg cagcagcagg ttacgtagaa gaacttgaga
    32521 atcccgctta cgtggtggtt ggtttagaca gtcaggtgac ctatgagatg ttggctatag
    32581 ccaccttagc tattcaaaaa ggtgccctct ttattgggac aaatcctgat ttaaacattc
    32641 caaccgaacg tggtttgatg ccaggagcag gtgcattgaa tgccttacta gaagctgcca
    32701 cacgtgtaaa acctgttttt attggcaagc ccaacgctat cattatgaat aaatcactag
    32761 aggtattggg cattcagcgc tctgaagccg tcatggttgg cgataattac ttgactgata
    32821 tcatggcagg tattcaaaat gatattgcaa cgatcctcgt gacaacaggc tttaccagac
    32881 ctgaagaggt accaacctta ccaattcagc cagatcatgt cctatctagc cttgatgagt
    32941 ggaggttgtg atggtagaaa acactaaatt actatgtagc tgggtatggt tattagcgtt
    33001 agctattctc ataacgatct attcaacgtg gctgtggtat cctcttgagg tggaccatct
    33061 aaagttagaa caagttgttt ttatgagcaa ggatgctatc ttgcataact ataacggact
    33121 actaaattac ctcaccaatc cttttgtgac aaggttggaa tttgctaatt ttcactcatc
    33181 agcagacgga ctcaagcatt ttgcagatgt gaagtggctt tttcacctaa ctcaagtcgt
    33241 ttttcttggc ctcctttacc caacccttaa gactttcaca caaaggttaa aaaccaaacg
    33301 tttctggctc cttcaaaaac ctttaatact agcagctttg tttcctctta tgattggtct
    33361 aatggctagt ttcattgggt ttgaacattt ttttactctt tttcatcagg tgctttttgt
    33421 aggagatagc agctggctct ttgatccctt gaaagatcct gtgatttgga tccttccaga
    33481 agtctttttc ttgcactgtt tccttttctt tatgatagtt tacgaaatca tcctgtggag
    33541 cttagtaggt ttagcaagat ggcagcgcct aaatgtagaa tgataaaagg attaagagat
    33601 taatttccct aaaaatgata aaacaagcgt tttgaaagcg cttgtttttt agtttgcagt
    33661 cagagtagaa tagaagtatc aaaaaaagca ccgactcggt gccacttttt caagttgata
    33721 acggactagc cttattttaa cttgctatgc tgttttgaat ggtcccaaaa caatatggaa
    33781 caaatcaaaa ttacaggaac gttttagagc tatgttgttt tgaatggtcc caaaacgtca
    33841 gacagcccac aaataatttt agatgggttt tagagctatg ctgttttgag tggtcccaaa
    33901 actagagtaa accgaatctt tgccatctct gggttttaga gctatgctgt tttgagtggt
    33961 ctccattcaa cattgccaat gataacttga gaaagagggt tcataccagc agtcggatac
    34021 cttcatattc tttttgttaa agcgttttca tgttataata gacaaaagaa gagtagagtg
    34081 atggaaccaa cattttttta tgattaagcc agatggggtt aagcgagagg gttagttgga
    34141 gagattttgc ggtggattga acgcctaaga tttacgtttg agcgattcga gctaagacaa
    34201 gctagttcga aatacttggc taagcacgac gagaccttgg tgataaacct tttgatccta
    34261 aacttaaagc ttacatgaca agtggtcctg ttttaattgg gataattctt ggggactaag
    34321 gtggttttgt ctttttgaac catgcatgat gagagtaacc aatcctacgg atgctctttg
    34381 cggaaccatt cgtgggaatt ttgctcaggc tcttggtgat gatggaggta tttttaacat
    34441 ggcgtatggg tctcattcaa gagactctgc taggcgtgag attgtccttt gggctcatca
    34501 aagtaatctc ggtagctccg ctattctctt gcaagataat ccg
//
LOCUS       DAUCWO010000011        33805 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUCWO010000011 DAUCWO010000000
VERSION     DAUCWO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33805
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.011"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            172..1626
                     /locus_tag="VGY20_000499"
     CDS             172..1626
                     /locus_tag="VGY20_000499"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ4273380.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRSLTIGFYQRLSDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            1744..2607
                     /locus_tag="VGY20_000500"
     CDS             1744..2607
                     /locus_tag="VGY20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4273381.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMVKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            2636..3286
                     /locus_tag="VGY20_000501"
     CDS             2636..3286
                     /locus_tag="VGY20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4273382.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            3419..4072
                     /locus_tag="VGY20_000502"
     CDS             3419..4072
                     /locus_tag="VGY20_000502"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ4273383.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESSHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            4204..5472
                     /locus_tag="VGY20_000503"
     CDS             4204..5472
                     /locus_tag="VGY20_000503"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ4273384.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(5530..6171)
                     /gene="plsY"
                     /locus_tag="VGY20_000504"
     CDS             complement(5530..6171)
                     /gene="plsY"
                     /locus_tag="VGY20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ4273385.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            6306..8255
                     /gene="parE"
                     /locus_tag="VGY20_000505"
     CDS             6306..8255
                     /gene="parE"
                     /locus_tag="VGY20_000505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ4273386.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEVLSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGAVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTIAERLKESAFLLKNVKMSLTDLRGDE
                     PIVEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDHDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVDSIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            8346..10805
                     /gene="parC"
                     /locus_tag="VGY20_000506"
     CDS             8346..10805
                     /gene="parC"
                     /locus_tag="VGY20_000506"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ4273387.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPPGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLKVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            10883..11950
                     /locus_tag="VGY20_000507"
     CDS             10883..11950
                     /locus_tag="VGY20_000507"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ4273388.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            12014..12244
                     /locus_tag="VGY20_000508"
     CDS             12014..12244
                     /locus_tag="VGY20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ4273389.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            12305..12385
                     /locus_tag="VGY20_000509"
     tRNA            12305..12385
                     /locus_tag="VGY20_000509"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12339..12341,aa:Tyr,seq:gta)
     gene            12436..12507
                     /locus_tag="VGY20_000510"
     tRNA            12436..12507
                     /locus_tag="VGY20_000510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12468..12470,aa:Gln,seq:ttg)
     gene            12634..13839
                     /gene="rpsA"
                     /locus_tag="VGY20_000511"
     CDS             12634..13839
                     /gene="rpsA"
                     /locus_tag="VGY20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ4273390.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            13952..14023
                     /locus_tag="VGY20_000512"
     tRNA            13952..14023
                     /locus_tag="VGY20_000512"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13985..13987,aa:Arg,seq:ccg)
     gene            complement(14386..14688)
                     /locus_tag="VGY20_000513"
     CDS             complement(14386..14688)
                     /locus_tag="VGY20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273391.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPKKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            14890..15876
                     /locus_tag="VGY20_000514"
     CDS             14890..15876
                     /locus_tag="VGY20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ4273392.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            16187..16573
                     /locus_tag="VGY20_000515"
     CDS             16187..16573
                     /locus_tag="VGY20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ4273393.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            16632..17540
                     /locus_tag="VGY20_000516"
     CDS             16632..17540
                     /locus_tag="VGY20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ4273394.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(17658..17834)
                     /locus_tag="VGY20_000517"
     CDS             complement(17658..17834)
                     /locus_tag="VGY20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ4273395.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            17968..18867
                     /gene="miaA"
                     /locus_tag="VGY20_000518"
     CDS             17968..18867
                     /gene="miaA"
                     /locus_tag="VGY20_000518"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ4273396.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            18940..20178
                     /gene="hflX"
                     /locus_tag="VGY20_000519"
     CDS             18940..20178
                     /gene="hflX"
                     /locus_tag="VGY20_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ4273397.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            20171..20806
                     /locus_tag="VGY20_000520"
     CDS             20171..20806
                     /locus_tag="VGY20_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ4273398.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            20821..21750
                     /locus_tag="VGY20_000521"
     CDS             20821..21750
                     /locus_tag="VGY20_000521"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ4273399.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            21750..22514
                     /locus_tag="VGY20_000522"
     CDS             21750..22514
                     /locus_tag="VGY20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ4273400.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAKEIADM
                     FQVNTLASIHFACLIGQKMADQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            22511..24721
                     /gene="recJ"
                     /locus_tag="VGY20_000523"
     CDS             22511..24721
                     /gene="recJ"
                     /locus_tag="VGY20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ4273401.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMTKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DESLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            24871..25389
                     /locus_tag="VGY20_000524"
     CDS             24871..25389
                     /locus_tag="VGY20_000524"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ4273402.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            25470..26153
                     /locus_tag="VGY20_000525"
     CDS             25470..26153
                     /locus_tag="VGY20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ4273403.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            26150..26806
                     /gene="nth"
                     /locus_tag="VGY20_000526"
     CDS             26150..26806
                     /gene="nth"
                     /locus_tag="VGY20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ4273404.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            26878..27564
                     /locus_tag="VGY20_000527"
     CDS             26878..27564
                     /locus_tag="VGY20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ4273405.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            27554..28342
                     /locus_tag="VGY20_000528"
     CDS             27554..28342
                     /locus_tag="VGY20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ4273406.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            28382..29488
                     /locus_tag="VGY20_000529"
     CDS             28382..29488
                     /locus_tag="VGY20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ4273407.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKIE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            29546..30415
                     /gene="rfbA"
                     /locus_tag="VGY20_000530"
     CDS             29546..30415
                     /gene="rfbA"
                     /locus_tag="VGY20_000530"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ4273408.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            30415..31008
                     /locus_tag="VGY20_000531"
     CDS             30415..31008
                     /locus_tag="VGY20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ4273409.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            31252..32292
                     /gene="rfbB"
                     /locus_tag="VGY20_000532"
     CDS             31252..32292
                     /gene="rfbB"
                     /locus_tag="VGY20_000532"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ4273410.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(32375..33514)
                     /locus_tag="VGY20_000533"
     CDS             complement(32375..33514)
                     /locus_tag="VGY20_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ4273411.1"
                     /translation="MWSEKHKSGKVNFVERYKHPYTGKWCRASVLMEKDTPRIRKEAQ
                     KYLDEKISKILTSLTTTDAYLLDVMNEWWEHHQKSLKSTSVRSLDFRIRELRNLIDPE
                     VMIAKITTKYLQSIIDKIPGSYDKRKRARQLLKQTFDYAIALEYVSINPVISTQLAKP
                     VKTIKDFEDVAQKFLEKDELKRLLDEMYRRKGSIKMAYLAEFMSLNGCRIGEALAIQP
                     DNIKNDIIEIHGTLDYTSNGYRNAIKTTPKTNSSWRETLITKREKEIIQDILKINALE
                     KNTNPNYKDMGYIFISRNGVPIQDNALNTSIRAANKRLEKPIQKELTSHIFRHTLVSR
                     LAENKVPLKTIMDRVGHADSKTTQQIYTHVTKSMKNEVVDILNRL"
BASE COUNT        10408 a         6033 c         7243 g        10121 t
ORIGIN      
        1 aatgaaaaaa tctctttaac cccaattcaa aatgttatgt tttgattgtt ttgtctacat
       61 tccttacaca tcacattcta atttctaacc ttttaatggt ggttttcttt tatttgtgtt
      121 ttaataaaaa agaatgtttt aggattcata tcttagaaag gaagagtagc tatgacttat
      181 caagatgcga cggctatggc tattgctgtt cagaccggtc agaccacacc actagaatta
      241 gtaacccagg ctatttataa ggctaaaaag ttgaacccta ccttaaatgc tattacctca
      301 gaacgctttg aagcagccct agaagaagcc aaacaaaggg atttttcagg taagcctttt
      361 gcaggagttc ccattttttt gaaagatttg ggacaagaat taaaaggtca ctcatcaacc
      421 tcaggttcca gattgtttaa agagtatcaa gcgactaaaa cggatttatt tgtgaaaaga
      481 ttagaagctt taggctttat tattttaggg cgttccaaca cgcctgaatt tggctttaaa
      541 aatattagcg atagcagttt gcacggacca gtgaatttac caaaggataa tacacgaaat
      601 gctggtggct catctggggg agctgctgct ctagtgtcat caggaattag tgcactagca
      661 actgctagtg atggtggtgg ttctattcga attccagcct cgtttaatgg cttaattggg
      721 ctcaaaccta gccgtgggcg catgcctgtc ggacctggtt cttatcgaag ctggcaagga
      781 gcctcagttc attttgcctt gaccaaatcc gttagagata ctagaaatct actctactat
      841 ttacagatgg aacaaatgga aagccccttc cccctagcca ccttaacaaa agatagtatt
      901 tatcaatcgc tacaacgttc cttgacaatt ggtttttatc aacggttaag tgacggtagc
      961 ccagtatctt tagatactgc aaaagcttta agacaagcag taacttggct aagagaacaa
     1021 gggcatcagc tagttgaatt ggaagagttt cctgtaaata tgacagaagt gattcgtcat
     1081 tattatatta tgaatagtgt ggaaacggct gctatgtttg ctgatattga agacactttt
     1141 ggacgtccga tgaccaaaga cgatatggaa accatgacct gggctattta ccaatcaggt
     1201 aaagacatcc cagcttggcg ttactcacag gtccttcaaa aatgggatac gtacagtgca
     1261 accatggcta gttttcatga gacttatgac ctgttgttga cgttcacaac caacacacca
     1321 gctcctaagc acggagaact tgtcccagat tcgaaactca tggctaactt ggctcaagct
     1381 gaaattttca gcagtgaaga gcaattcaat ttggtcgaaa ccatgtttgg taagagtctg
     1441 gctattaatc cttacacagc tctgcctaat ctaactggcc agccagctat tagtttacct
     1501 acttacgaaa ctaaggaagg gctcccaatg ggcattcaac taattgcagc caaaggtaga
     1561 gaggacctat tattaggaat tgcagagcag tttgaggcag caggattatt gaagattcct
     1621 caataataat cttataaaac agtttaacta ttatagatta actttttaaa gagattgagc
     1681 gctcactgat aacacaactt aatcaaaatg ttatactatt cttatcttaa taaaggagtt
     1741 actatgaaga aatcagttac tttactgtct atcggacttg ccagcctttt attggcagca
     1801 tgtgcgccac ataaatctca aaaatcaagt tgggacacta tcaaagaaaa aggggtgtta
     1861 aaagtagcta ccccaggaac ctatcagcca acttcttttt acaacgataa taatgagtta
     1921 gtaggttacg aagtagatat ggtcaaagaa atcggtaaac gacttaacat taaagtcaag
     1981 tttgttgaaa caggatttga ccaagcgttt acctcagttg atagtggtcg agtggatatt
     2041 tctttgaaca actttgacat taccccaaaa cgtcagaaaa aatacaatat ctctacgcct
     2101 tataaatacg gggtaggagg catgattgtc cgtgctgatg gcagctcaaa catggttaaa
     2161 aaagatctta gcgactggaa aggaaaaaaa gcagctggtg cttctggaac agaatacatg
     2221 aaagtagcgc aaaaacaggg agctgaactg gtgacctacg ataatgttac aggagatgtt
     2281 tatctaaacg atgtggctaa cggtcgaaca gatttcattc caaatgatta cccagctcaa
     2341 aaattatttg tggattacat gttatctcaa aatccaaatc taaatgtcaa aatgagcgat
     2401 gttcagtaca acccaaccga acaaggaatt gttatgaaca aaaaagacga tagcctcaag
     2461 aaaaaaatag atgctgttat taaagacatg ataaaagatg gtagcttgaa gaaaatctct
     2521 gaaacctact atgctggtca agatttgaca aaaccttttg gcaaagacaa aaaaattccc
     2581 gtcattgata cgaaagacgt taactaatac ttgatgttaa aggagtccta accacatgat
     2641 taatatccca ttaatgaaag atagtttagg atttgtgtta tcgggattac cttataccct
     2701 tggtatttcg ttgctcagtt tttttacggg tctgttttta ggtttaggcc tagcccttct
     2761 gggaaggtca catcagccac tgctacacta tcttgttagg gcctacatct ctattatgcg
     2821 aggtgtgccg atgatcgtgg tgctctttgt tctttatttc gggttgcctt attatggttt
     2881 agaattgcca gctttacttt gcgcttatct tggtttttct atggttagcg ctgcctatat
     2941 ttctgaagtt tttcgctctt ctatagaagc tattgacaag ggacagtggg aagcagctaa
     3001 ggctttaggg ttgccatatg ctcttatggt taagaaaatt attcttcctc aagcctttag
     3061 aattgcagtt cctcctctag gcaatgtcat tattgatatg gttaaaagtt cgtcgctagc
     3121 tgccatgatt accgtaccag atatttttca aaatgccaaa attatcggtg gtagggagtg
     3181 ggactacatg tccatgtaca ttttagtagc ctttatctat tggattattg cttttttgtt
     3241 agaacgttac caagaattct tagaaaataa gctagcactt gtttaatatc ctgtaaaaaa
     3301 cgaatattaa ccctggaaat atcatatttt cagggtcttt ctatttaaaa tgtggtaaac
     3361 tagatagaaa aggacatcgt atttaactta gaaactgacg tgattagaaa ggaatcttat
     3421 ggctcattca atttggcatg agaaaatcaa atcatttttg ccggagcatt actacggtcg
     3481 catcaatcac tttttagatg aagcctatgc ttccggtctt gtctaccctc cacgagaaaa
     3541 tgtctttaaa gccttacaag tcactccttt ggaagaaacc aaagtgttga ttttaggaca
     3601 agacccttat catggcccca aacaggccca gggcttatca ttttcagttc cagaagagat
     3661 ttctgctccg ccatccctta ttaatatttt aaaagaatta gcagatgaca ttggtcctcg
     3721 tgaccatcat gatttaagca cttgggctag tcaaggggtt ctgcttttga atgcttgttt
     3781 aacagtgcca gcaggacagg ctaatgggca tgcaggctta atatgggagc catttacaga
     3841 cgctgttatt aaagtgctga atgagaaaga cagccctgta gttttcattt tgtggggagc
     3901 ttatgcaagg aagaaaaaag ccttcattac taatccaaag caccacatca tcgagagctc
     3961 tcatccaagc cctttgtcat cttatcgtgg cttttttggc agcaaaccct tttcaagaac
     4021 caacgctatt ttagaaaaag agggcatgac tggcgtagat tggctaaaat aagccagcca
     4081 tttcaactaa atagcttaag ctagacattt tgataccttt aataagtgaa atgagtgcag
     4141 tagatttttc ctattaaaga acgtaaggtc ttatggttaa aacagataaa taaaggagaa
     4201 aacatgatac tcatcaaaaa tggacgcgtt atggacccga aatcgcagag agatcaagtg
     4261 gcagatgtct tgattgatgg taaacagatt gtaaagatag cttcagctat tgaatgccaa
     4321 gaagctcaag tgattgatgc tagtggtctt attgttgccc cgggtttggt cgatattcat
     4381 gtgcatttta gagaacccgg tcaaacgcac aaagaagata ttcacacggg agccttggca
     4441 gcagcagcag gtggtgtgac tacggttgtc atgatggcta ataccaatcc tgtcatttca
     4501 gatgtcgaaa ctttacagga agtgctagct agcgcagcta aagaaaagat tcatatttac
     4561 accaatgcca gtgtcaccca agcctttaat ggaaaagacg tgactgattt taaagctttg
     4621 ttggaagcag gagcagtaag cttttcagat gatggtattc ccttagaaag ttctaaagtt
     4681 ttaaaagaag cctttgattt ggctaatgcc aaccaaactt tcatttcttt gcatgaggaa
     4741 gatcctcagt taaatggggt tcttggtttt aatgaaggta ttgctgagga gcattttcat
     4801 ttttgtgggg caactggtgt agcagaatat agtatgattg caagagatgt catgattgct
     4861 tatgacaggc aagctcatgt tcatatccaa catttatcta aagcagaatc ggttcaagta
     4921 gtggcttttg ctcagcagtt gggtgctaag gtaactgctg aagtgtcccc acaacatttt
     4981 tcaacaactg aagatctctt attgatcgca ggtaccagcg ccaagatgaa tcctcccctt
     5041 cgtacacaaa gagatcgctt agctgtcata gaaggcttga aatcgggtgt tatcacagtc
     5101 attgccactg atcatgctcc tcatcacaag gatgaaaaag ctgtggatga tatgactaaa
     5161 gccccatctg gtatgacagg acttgaaact tctttgtcat taggtttgac gcatttggtt
     5221 gaaccaggtc atcttacact tatgtcacta ttagaaaaaa tgacgcttaa tccagctttg
     5281 ctttatggtt ttgatgcggg ttatttggca gaaaatggtc cagcagattt ggttattttt
     5341 gcagacaagc aagaacgtct gatcactgag aattttgctt ctaaagctag taattcccca
     5401 tttattggta acaaactcaa aggtgtcgtg aaatacacta ttgcagacgg agaggttgtt
     5461 taccctaatt aacaaatgag ttaaaacaaa aaagcttgac tttcagagaa agtcaggctt
     5521 ttagtaaatt tattttttag gcacttgttt gcttaaattt agcccccaag gtattagatt
     5581 ttcagtatga tgtttgatac gactaatgtt atctttgtgt cttataataa taatgaacgc
     5641 aagtaatatc acaataaaag ttaaaaagta gtcataattt ggtaaaagaa aatgaatggc
     5701 aggaaatgtt aaaacagata acacaccaac gatagctgaa accacactag ctaaagatat
     5761 catactaaat aaatagagaa ctaaaacaaa gatagatgct aaaaaaaaga gatataacgg
     5821 agcaaagcct agcaatacac cagcacttgt tgctacggcc ttaccacctt taaagttggc
     5881 aaaaatagga aaagtatgcc ctaaaactgc gaaaaagccg atagcaatgg atgaaattga
     5941 agtcatacca aaaataattg gtaacaatat tgaaagtgtc cctttaaaca tatcaatagc
     6001 taaggtagct gttcctgcct tgacacctaa aatccgaaaa gtatttgtgg ttccagtatt
     6061 tcctgatcca tgctctcgta agttgatgtg gtaaaagtac tgtccaatcc atagtccagt
     6121 tggaatagaa ccaagtaaat aggcaatggt aataaaaagt agtaatttca taatccttat
     6181 tataacatat tcatagattg gagaactaga tctggaaaag aattcggaaa aaattagcaa
     6241 attattttgc aaaattggta aaaaacttgt aagataaaaa agatgaaact gtctggaggt
     6301 cggtgttgac taagaaagaa attacaatta ataattataa tgacgatgct atccaagtcc
     6361 tagaagggtt agatgctgtt cgcaagcgtc cagggatgta tatcggttca acagatgcta
     6421 cgggattgca ccacctgatt tgggaaattg ttgataatgc tgtcgatgag gtcttgtctg
     6481 gttttggaga tgatattaag gttgttatca ataaagatgg ctcggtcagt gtggcagata
     6541 gcggacgtgg aatgccaaca ggtcagcatg ccatggggat tcccaccgtt caagttattt
     6601 ttacgattct tcatgcaggt ggtaaatttg gtcagggtgg ctacaaaacg tcaggtggtt
     6661 tgcacggtgt tggctcctct gttgtcaatg ctttgtctgc ctggttagaa gtcgaaatca
     6721 caagagacgg ggcagtgtat cgtcagcgct ttgaaaatgg tgggaaacct gttacaaccc
     6781 ttaaaaaggt tggtacagct cctaagtcta aatcaggaac tgtcgtcacc tttatgcctg
     6841 atgataagat tttttcaacc attgacttta agttcaatac cattgcagaa cggcttaaag
     6901 aatctgcttt tcttttgaaa aacgttaaga tgagcttgac agatcttcgt ggagacgaac
     6961 cgattgttga agagtttcat tatgaaaatg gggttcaaga tttcgtcgaa tacctcaatg
     7021 aagataagga aaccttgaca cctgttattt acatggaagg tcaagaccac gactttcaag
     7081 tcgaggttgc tttgcagtat aacgatggtt tctccgacaa tatcttatcc tttgtaaaca
     7141 atgtgcgcac caaagatggt ggaagccatg aaacgggact gaagtcagct attaccaaag
     7201 cgatgaatga ctatgctcgt aaaaccaatc tcttaaaaga aaaggataaa aatctagagg
     7261 gctcagatta tcgagaaggg ctctcagcag tattatctat cttagttcct gagcagcact
     7321 tgcaatttga aggacagacc aaagacaaat tgggaagtcc cttagcgaga ccgattgtcg
     7381 atagtatcgt ttctgaaaaa ttaaccttct ttttactcga aaatggagaa gtagcttctc
     7441 atctagttcg caaagccatt aaagctagag atgctcgcga agctgctcga aaagcgcgtg
     7501 atgactcacg caatggtaag aaaaacaaaa aagacaaggg cctactatcc gggaaattga
     7561 ctcctgccca gtccaaaaat gccaagaaaa atgaattgta tctggtcgaa ggagattctg
     7621 cgggaggttc agccaaacaa ggacgagacc gcaaatttca ggctatttta ccattacgtg
     7681 gcaaagtttt aaataccgaa aaagctaaaa tggctgacat cttgaaaaat gaagaaatta
     7741 acactatggt ttataccatt ggagctggag tgggagctga ctttaatctc gaggatatca
     7801 actatgataa gattattatc atgaccgatg ccgataccga tggtgcccac attcaaaccc
     7861 tcttattgac cttcttttac cgttatatgc gaccattagt agaaacaggt cacgtttata
     7921 ttgcgcttcc cccgctttac aaaatgtcaa aaggtaaagg caaaactgaa aaaattgctt
     7981 atgcttggac tgatggtgaa ttggaagatc tgcgcagaga atttggcaag ggggctattc
     8041 ttcaacgcta taaaggactg ggtgaaatga atgctaacca attatgggaa accactatgg
     8101 accctgaaac cagaaccctt attcgtgtta ctattgacga cttggcaaga gcagagcgac
     8161 gtgtttctgt gttgatgggt gacaaagcag ccccacgccg tcagtggatc gaggataacg
     8221 tcaagtttac cttagaagaa aatacggtgt tttagtcata caagtttctc acagataatt
     8281 acaagtgcta aagacgattg ttttttaggt aattaataaa taatgaaaaa gaaagctatt
     8341 tgaatatgtc aaacattcaa aacatgtccc ttgaggatat tatgggagaa cgcttcggcc
     8401 gttattccaa gtatattatt caggaacggg cacttcctga tattcgagat ggattgaaac
     8461 ccgttcagcg tcggattctt tattcgatga ataaggatgg caatactttt gaaaaaggtt
     8521 accgtaaatc agccaaatca gttggtaata ttatggggaa tttccaccca catggggatt
     8581 cctctattta tgatgccatg gtacggatga gtcaagattg gaagaaccgt gaaatccttg
     8641 ttgaaatgca cggaaataat ggttcaatgg atggtgatcc gcctgcggct atgcgttata
     8701 cagaagctcg cttgtctgaa attgcgggtt atcttcttca agacattgag aaaaatacgg
     8761 tctcttttgc ttggaacttt gatgacacag aaaaagaacc caccgtttta ccagcagctt
     8821 ttcctaactt acttgtcaat gggtcttctg gtatttccgc aggctatgcg acagatattc
     8881 caccgcacaa tttgtcagaa gtgattgatg ctgttgtgta tatgattgat catcccaaag
     8941 ctagtttaga gaaattaatg gagtttttac caggaccaga tttcccgcca ggaggcatta
     9001 tccaaggagc tgacgaaatc aaaaaagcct acgaaacagg taagggacgt gtcgttgttc
     9061 gctctaggac agaaatcgag gaacttaaag gtggtaaaca acaaattatt gtcactgaga
     9121 ttccttatga agttaacaaa gcagtcttgg tcaaaaagat tgacgatgtg cgtgttaaca
     9181 ataaagtccc aggcatcgta gaagtacgcg atgaatctga tcgcacaggt cttcgcatag
     9241 ctattgagtt gaaaaaagag gctgatagcc aaaccattct aaattatttg ttgaaataca
     9301 cagaccttca agttaattat aattttaaca tggttgctat tgaccacttc acaccacgtc
     9361 aagtaggact tcaaaaaata ctatcaagct atatcagtca ccgtaaagat attattattg
     9421 agcggtctaa attcgataaa gccaaagctg aaaaacgcct ccacattgtt gaaggattga
     9481 ttcgtgtctt gtccatctta gatgagatta ttgctttgat tagatcgtct gacaataagg
     9541 ctgatgctaa agaaaatctg aaagtcagct atgatttctc tgaggaacaa gcagaagcga
     9601 ttgtaacctt gcagctttat cgtttaacta ataccgatat tgttactctc caaaatgaag
     9661 aaaatgattt aagagactta atcacgaccc tttccgctat tatcggtgac gaagcaacca
     9721 tgtataacgt catgaaacgt gaattaagag aggtgaaaaa gaaattcgcc aatcctcgtt
     9781 tatcagagtt acaagcggaa agtcaaatca ttgagattga cactgccagc ttgattgcag
     9841 aggaagaaac ttttgttagt gtgacacggg gcggatacct caaaagaacc agcccacgat
     9901 ccttcaatgc gtctagttta gaagaagttg gcaagagaga tgatgacgaa ctcatcttcg
     9961 ttaaacaggc caaaacaaca gaacaccttt tgttgtttac aactttaggc aatgtcattt
    10021 accgaccaat ccatgaattg accgatttac gttggaagga tatcggtgaa catttatcac
    10081 aaaccatttc aaacttcgcg acagaagaag agattctcta cgctgatatt gtgacttcct
    10141 ttgaccaggg cttgtatgtg gctgtcacac aaaatggttt tatcaaacgc tttgatcgta
    10201 aagaactaag tccttggcga acttataaat ctaaatcaac caaatatgtg aagctaaagg
    10261 atgacaaaga ccgcgtggtt accttgtcac cagttattat ggaagacctc ctattagtga
    10321 ctaaaaatgg ttatgctttg cgattctcca gtcaggaagt gcctattcaa gggttgaaat
    10381 ctgctggtgt taaaggcatt aacttgaaaa atgatgacag ccttgcctca gcctttgctg
    10441 tgacaagtaa ctctttcttt gtcttaactc agaggggttc tctcaaacgg atggctgtcg
    10501 atgatattcc tcagacaagt cgtgccaatc gtggactatt ggtcctacga gagttaaaaa
    10561 ccaaacctca tagagttttt ctggccggag gcgttcaatc agatacttca gctgagcagt
    10621 ttgatttgtt cactgatata cctgaagaag aaactaatca acaaatgctt aaagtcatta
    10681 gtaagactgg tcaaacctac gagattgctt tagaaacgct ttcgttatcg gaaagaacca
    10741 gcaatggctc ctttatttca gacactattt cggatcaaga agtactggta gctagaacac
    10801 gttaaaaaga gtgaaaactc tttttaattt tatggtaaat ttagaatatt aatgcaaaag
    10861 agtagaataa acttgaaaat tattgaaaaa aagttacaat agaaaaaagc agaaaagagg
    10921 tacgatgatg acaatagcaa ttgattggga caacctagga tttgaatatc ataagttgcc
    10981 atttcgttac atttcttatt acaaaaatgg tcaatgggat aaaggtcaat taacagaaga
    11041 tgccacctta catatctcag aaagcgctcc agcgcttcat tatgggcaac aagcttttga
    11101 aggtttaaag gcttatcgca ccaaagatgg ctctattcaa ctatttagac cagaccgtaa
    11161 tgcagtgcgt ttacaagcca cagcagaccg cttgttgatg ccacaagtat caacagagca
    11221 atttattgat gcagctaagc aagtggttaa agccaatgaa gactttgtac caccttacgg
    11281 aacaggtgca acgctttacc tgagaccgtt attaattgga gttggagata ttattggggt
    11341 taaaccagcc gaagaatata ttttcaccat ttttgccatg ccagtaggaa actatttcaa
    11401 aggtggactt gctccaacta attttattgt ttctgaagcg tttgaccgcg ccgctcctta
    11461 cggaacaggt gctgctaagg tgggaggaaa ctatgcagga agtttgctac caggtaaggc
    11521 cgctaaatca gcaggctttt ctgatgtgat ttacttagat ccagcaaccc atactaaaat
    11581 tgaagaagtc ggagctgcaa acttttttgg cattactgca aataatgaat ttgttacgcc
    11641 acttagtcca tccatcttgc catctattac gaagtattca ctcttacaac tagctgaaga
    11701 aagacttggt atgacagtaa ttgagggtga tgttccaatt aatgaattgg ataaatttgt
    11761 ggaagcagga gcttgtggaa cagcagctgt tatctctcca attggcggta tccagtacaa
    11821 ggataactta catgtctttt acagtgaaac agaagttggt cctgtgacac gccgtttata
    11881 cgatgagttg gtcggcattc aatttgggga tattgaagca ccagaaggtt ggattgtaaa
    11941 agtagattaa aatagagctt gctcttgcaa gcttttttga ttttgtgtaa aatagacaaa
    12001 gtgaggtaaa gtgatgagta aaaaagataa aaaaattgaa atccaactga ttgatcacaa
    12061 agttatggtt aatgagacta agattgatgg ctatcaatta caaattggta aacgtgtggt
    12121 tggagaaatt gcagaacttg atgagaaatt tgctgtctta aaaaatgatg gagtagattg
    12181 cttcttcaaa acgctcgaac aagcggttga aaatatcatt gaaaactaca atttgaacca
    12241 ttaaaattag acaaaaacac ttgccagctt acaaaaacta tgatagaata gtttttgttg
    12301 ttacggaaag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc cttcgggttc
    12361 gggggttcga atccctctct ttccatttcg gaaactaatc taattagaaa attagttttc
    12421 tgtaaaaaat atttttgggg tatagccaag cggtaaggca agggactttg actccctcat
    12481 gcgttggttc gaatccagct accccagtca gaggtatcag gtgctccctg agaccgtcaa
    12541 agataagagt tgttgtcgtt gcgggttacc ttgagaaaat atattgttat caaaagctag
    12601 cattggctag cttttgttgg aggatttttt agaatgaatg aatttgaaga tttgctaaac
    12661 agtgttagcg aagtgaaccc tggtgatgtt gtcactgcgg aagttttaac tgttgataac
    12721 ggtcaagcaa acgttgttat cgaaggaaca ggtgttgaag gtgtcttgac acttcgtgaa
    12781 ttgactaacg accgcgatgc tgatattaac gactttgtta aagctggcga cacagttgaa
    12841 gtacttgttc ttcgtcaagt agtaggtaaa gatactgata cagttacttt cctagtatct
    12901 aaaaaacgtt tggaagctcg taaagcctgg gacaaacttg ttggtcgtga aggcgaagtt
    12961 gttactgtta aaggtacccg tgctgttaaa ggtggtcttt cagttgaatt tgaaggactt
    13021 cgtggattta tccctgcttc aatgattgac actcgttttg ttcgcaacac tgaaaaattt
    13081 gttggacaag agtttgatgc taaaatcaaa gaagttgatg cagcagaaaa ccgtttcatc
    13141 ctatcacgtc gtgaagttat cgaagaggcc gctaaagaag ctcgtgctga agtgttctct
    13201 aagatttcag aaggtgcagt cgttacaggt actgtagcgc gtttaacaag ttttggtgct
    13261 ttcattgacc ttggtggtgt tgacggactt gttcacgtga ctgaattgtc tcacgaacgt
    13321 aacgtgtcac ctaaatcagt tgtttcagtt ggtgaagaag ttgaagttaa agttctttca
    13381 attgacgaag aagctggtcg tgtgtcactt tcacttaaag caacaacacc tggaccatgg
    13441 gacggcgttg aacaaaaact tgctcaaggt gatgttgttg aaggtaaagt aaaacgcttg
    13501 actgacttcg gtgcttttgt tgaagtatta ccaggtattg atggacttgt tcatatttca
    13561 caaatttcac acaaacgtgt tgaaaatcca aaagatgtac tttctgtagg acaagaagta
    13621 acagttaaag ttcttgaagt gaatgcagct gatgagcgcg tatcattatc aatcaaagct
    13681 cttgaagaac gcccagcgca agctgaagga gacaacaaag aagaaaaacg tcaatcacgc
    13741 ccacgtcgtc caaaacgtga atcaagacgt gactacgaac tcccagaaac acaaactgga
    13801 ttctcaatgg ctgatctttt cggtgatatt gaattgtaat aaatctaaaa agttgaggtt
    13861 ttcctcaact ttttattgtc ttctggttag aaaagttatt tcatattatt tcattttatg
    13921 atatactata agggtattga caaagttgtg accaccttta gtgtaatgga tatcacgtaa
    13981 gattccggtt cttgagatgg gggttcgatt ccctcaaggt ggatagaaat catataaaaa
    14041 agccctattt gataggcttt ttttgtttct tttccaaaat ttgtccaaaa ttatctagaa
    14101 gagttgacga accttttcaa aatcattatt tttctgacct tgaaaacgat gagtataggt
    14161 cttaaatgtc tctgtaatgc cacagcctga attttatttg actcagattc ataagattgt
    14221 aagtaatcaa ggttaattat tgatgagatg gtgaaaccat ttttagcaag tgctattaaa
    14281 gcatttagtg atttttcgaa acgttttaaa atttaagaag agttttacct aaaaaactag
    14341 ctgtgtcatc tatctaagca cagttagttt tttagtatta gatttttaaa gaagaaacgt
    14401 ctcaatctta attggataat ccttatattt ggattttaat atagtagcta cttcttgata
    14461 tagtatccga atctgcgctc tttttttagg catgttaaaa ccaacataat ggaattgtga
    14521 ttcaatagtt acaaataaat catggaacag gtgattcata gtttctaatt tatggcaaag
    14581 cagtcgatct tcttgaatga atgttggggg attttgatct tctccccagt aagcatcaac
    14641 acgtgacaac ggataagtcc catattctaa acaaagttca taagtcattt tggcctcctt
    14701 gttttatctt agcattttct aaataaagct tctaatgatt tgcttaaagg agtaatgggt
    14761 tgccatctca tctcatcaaa aataaattga gatctatttt gtgaaaaaaa aagacaaaca
    14821 aatggcttgc ctagacttgt ttttcctaga tataatagga agtaactgaa taacaatgaa
    14881 ggagaaaaaa tgtcagaaaa aatcagagtt ttactctatt ataaatatgt ttcaattgaa
    14941 aatgcgcaag agtacgctgc gaaacatcta gaattttgta aatctattgg gcttaaaggt
    15001 cgtattttga ttgcggatga ggggattaat ggaactgttt ctggagatta tgaaacaact
    15061 cagaagtata tggattgggt ccacagcgat gagcgttttg ctgatctttg gttcaagatt
    15121 gacgaggaaa atcaacaagc tttccgtaaa atgtttgttc gttataagaa agagatcgtg
    15181 caccttgggt tagaagacaa taactttgat agtgatatta atcctttgga aacaacaggt
    15241 gagtatttaa atcctaaaca atttaaagaa gccttgcttg atgaagatac tgttgtttta
    15301 gatacgcgta atgattatga gtatgactta ggtcacttcc gtggagcaat tcgtcctgat
    15361 attcgcaatt tccgtgagtt accacaatgg gtacgtgaca acaaagataa gtttatggaa
    15421 aaacgtgttg ttgtgtactg tacgggtggt gttcgctgtg agaaattctc gggttggatg
    15481 gttcgagaag gctttaaaga tgttggacaa ttacacggtg gtattgcaac atatggaaaa
    15541 gatccggaag ttcaaggtga actttgggat ggtgcgatgt atgtttttga tgatcgtatc
    15601 tcggtgccaa ttaatcatgt taatccaact gtcattagta aagattattt tgatggaaca
    15661 ccttgtgaac gttatgtcaa ttgtgcaaat ccgttttgta acaaacaaat ttttgcttca
    15721 gaagaaaacg aaacaaagta tgttcgagga tgctcgcctg aatgtcgtgc acatgaacgt
    15781 aatcgctatg tgcaagaaaa tggtttatca cgtcaagaat gggcagaacg tttagaagct
    15841 attggagaat ccttgccaga atttgtaggt gcctaatatc tcaaaacaaa tcaggaaact
    15901 ggtttgtttt ttgtgataca gatgatgaga ccaaattgca aagactttgc aacaagatac
    15961 tagtgaacgt ggacaccaag ctcattttgt catgcagagc catcaacagc gaagacgtca
    16021 acaccaactc tgtctagaac cttttcaaaa gaattgtgag tttctgtatt acgagataga
    16081 gaaggtaaaa ctggattctg aagactgcct taccatcaat cctttgggaa tggtttaagc
    16141 tttacagctt gatgatggtc aaatcgtcac acaggcaggt attatcttgc tctttatagc
    16201 gaaacaaacc ctgaaatctc agctcattcc ccaagataat cttttggcag aaagtcgatt
    16261 caacgaaatc atggatttct taacaggtga ttttcccctt gtttttaggc caatgtacaa
    16321 ccctcatcgc tacactattt ctcaagacaa tcaagcgctt gaaaaagtca aacaagcttc
    16381 ttacaagcgg atggatattg ctatgacgca tttagatggt cttattgggg atagtgggca
    16441 tgtttatcga gatcaacaaa ccattgcaga tgcttatgct tacgccatgg cgctatggtc
    16501 tcaaaaaaca ccaaaatctt acgagaatta tccgcatctg gcagccttta tggcaaaaat
    16561 gctcgaaatg tgataaaata aacaagatta tgtcactaaa aatagctgtt aataggcaga
    16621 aaaggacact tatgaaacac cttaaaaacc caccattggt catgtcagga ttggcattag
    16681 gaacgttgtc atttggtaat ttgttggcta cttatgtttc gatttttagt tatttgggaa
    16741 ttctggcagc tctttttatc tatggtatct tgcttgttgg catggttagg aaccttaacg
    16801 ataccaaaat gcagttacga caacctctaa ttgcctctgt ttttccaact ttttttatga
    16861 cagggatgtt gttgtcttca ctctttctca aagtgacagg agggtgttgg ttaggctttc
    16921 tcacttggtg gctattcttt ttgggaaatc ttgtcctgat tgcttattac cagtatcgtt
    16981 ttgtcttttc tttttcatgg gataacgttt ttccttcctg gtccgtgctt tttgttggaa
    17041 ttgcaatggc ggcattaaca gcacctgcta gtaggcagtt tttattagga caggtgattt
    17101 tttgggtttg tcttctctta actgctgtga ttttaccttt tatggccaaa aagacttatg
    17161 ggattggttt aggtcaagca gtaatgccca atatttcaac gttttgtgcc cctctgtcct
    17221 tactttcagc gtcttattta gcaacctttc ctagaccaca agtaggaatg gttatctttc
    17281 ttctcatcag ctcacaactg ttatatgctt ttgtggttgt acagcttcct cgcttactca
    17341 atcgtccctt taacccaggt ttttcagctt ttacctttcc gtttgtgatt agtgccactt
    17401 ccttaaaaat gaccttatct tttttaggtt ggcaaggttt aggttggcag gttcttttgc
    17461 taggagaggt attgttggcg acagctttag tcacgtatgt ttatggggct tacctgagat
    17521 ttctatttca aaataagtaa agcagctaac atcttggctc atcaagagac agttactact
    17581 aactagttat cgagattact aaaaaagagg ccggcacttt aatgtgctag tctcttttaa
    17641 attgatccat tgtcacgtta gcgagctaca cgacgacgcg cagctttttt gcggttttct
    17701 tcaatgaaag cagctttttc ttcttcaggg tcaatgattg ttttttttac agcaaagaca
    17761 gcaccagcaa cagtagcagc agttgctagg acgccagtag caagaccttt aacaaattga
    17821 tatttcttaa ccatgattat tctccatttc tagtgaagga cctcagtggt ccttaatctt
    17881 atgttataat atatggtagc gaaaaataag ctaattttca agtaaaaggc gtttttggac
    17941 acgcttttct aatagaaaga caagaaaatg acaaaaataa aaatagttgt gattgttggt
    18001 ccgacagcgg ttggaaaaac agcacttggg attagtttag ccaaggcttt taatggagaa
    18061 attatttcag gagatagcca gcaagtttat cgacagttgg atattggaac agccaaggcc
    18121 actcaagaag agcaagaagc agcggttcat cacttaattg atattcgtga ggtgacggag
    18181 tcttattcgg cctacgattt tgttcaagat gctcaaaaag ctatttcaga cattgttagt
    18241 cgtgggaaat taccaattat tgtaggtgga actggtcttt acttacaaag tttactcgaa
    18301 ggttatcatc taggaggtca agttgatcaa gaggcggtaa aagcctatcg gaatgagttg
    18361 gagcaattgg acgatcacga cttatatgaa cgcttgcaag tcaataacat taccattgag
    18421 caggtgaatc gcaggcgggc gatccgagcc cttgaattgg ctcagtttgc tgatgaatta
    18481 gagaatgccg agacggctta tgagccgctt attatcggct tgaatgatga ccgccaagtc
    18541 atctatgacc gtatcaatca gcgcgtggat cgtatgcttg aaaacggctt attggaagaa
    18601 gcaaaatggt tatatgagca ttacccgact gttcaggcta gtcgtggcat aggttataag
    18661 gaattattcc cctattttgt aggtgaaatg actttggcag aagctagtga tcagttaaaa
    18721 caaaacacga gacgttttgc caagcgacag ctaacttggt tccgcaatcg aatggctgtt
    18781 agcttcactg ccataacggc tcctgattac ccacaggtag tacatgatag ggtcagggat
    18841 tttcttggtc agaaagagaa gtcatgacgc aaaattagca gaatctgttg aagatatgtt
    18901 ataatagaat ttacggaatt tggaggattg gtaaaggtga tgattgagac aaagcgtcag
    18961 caagaaagag tgattctttt aggagttgag cttcagacaa cagaacattt tgatatgtcc
    19021 atgacagaat tagctaatct tgccaaaaca gctggcgtca aagttatggc aagtttcagc
    19081 caaaaacgag aacgttacga cagcaaaacc tttatcggct cagggaaact ggatgagatt
    19141 aaggcgattg tggaagctga tgagattgat gcagttattg tgaacaatcg gttgactgct
    19201 aggcaaaatg ctaatctaga agctgtttta gaggtaaaag tcattgaccg tatgcagtta
    19261 atcttagata tttttgccat gcgtgccaga agccatgagg gcaaattaca agtacacctt
    19321 gcccaattga aatatatgtt acctcgtttg gttggacagg gcatcatgtt aagtcgtcaa
    19381 gcaggtggta ttgggagccg tggaccaggt gaaagccaac tagagttaaa tcgccgttcg
    19441 attcgtcatc agattgctga tattgaacgc caattaactc aggtagagaa aaatcgtcaa
    19501 acaattagag atcgtcgtgt gggttcagat acctttaaaa ttggcttgat tggctacacc
    19561 aatgctggca agtcaaccat tatgaacttg ttgacagatg acagccacta tgaagccaat
    19621 gaattatttg ctaccttaga tgcaactaca aagcaactct atttagaaaa tcaatttcaa
    19681 gcaaccttaa cagacaccgt aggctttatt caagacttgc caacggaatt agtggctgct
    19741 tttaaatcaa ccctagaaga aagcaaatat gtggatttgc tgttacatgt gattgatgcg
    19801 agtgatccca atcatagtga gcaagaaaaa gtggttttaa accttctaaa agagctagac
    19861 atgcttaata ttccacgttt agctatttat aacaaggtgg atatagcaga acaattcaca
    19921 gctactgctt ttccaaatat caggatttca gcccgttcaa aggatagcaa aattctcttg
    19981 agacgtttaa tcattgacca aattcgagac cagtttgttc cttttagaat caaagtgcat
    20041 caagacaagg cgtacaagct gtatgatttg aaccgagttg ctcttttaga ccactacacg
    20101 tttgaccaag aaatagagga tatttcgggc tacatttccc caaaacagca atggagactt
    20161 gacgattttt atgaataatt atattgattt agcaaaaacc tatggtggat ttactagtct
    20221 ggataccaac tacctaaacc atctattagc cagtttgaca gaccagcaaa aactagcttt
    20281 tatcacacca ccaccaagtg ttattaatgc ctactttgct gaaatctacc aaaaacagtc
    20341 accgcaagct gcgactgatt attattttaa tctttctaag gctttggggc tttttactga
    20401 ccaaccatct tttgaggaag aaaagccctt tgtacgcctc aatttgtctg gaaaagctta
    20461 tggttttgct taccaaaatg accaagaagt tgcattggta ttttctgaaa aagcagagcc
    20521 taagaaaccc gaactcttct ttgaattaac acaaattttc ccacaatata tggtttatga
    20581 agacaaaggc cagcttaaaa tgcaggctaa gcagtttgag caaggagaat gtgaagacat
    20641 tacgcctgat gacacactct tgtcgaagat ttaccgtctg gcaaacggta tcaccatgct
    20701 taaggggttt aatgtagagg agttatgggc tcttagccaa acattttctg gtcaaaagta
    20761 ttatgatttt gcccaacgag aatttatgat ttacatcacc caatagaaag aatagattga
    20821 atggaattac aatttttagg aactggagct ggtcaaccag ccaagcagcg caatgtgtca
    20881 agtcttgcct tgaaattact agacgaaatc aatgaagtct ggatgtttga ttgcggtgaa
    20941 ggaacgcaaa gacaaatttt agaaacgacg attaagccac gaaaaataag aaagattttc
    21001 attactcatt tgcatgggga ccacattttt ggattaccag gatttttatc aagtcggtct
    21061 tttcaagcta gtgaagaaca gacagatctt gatatttatg gaccaattgg cattaaaact
    21121 tacgtgttaa ctagtctaaa agtttctggt gcgcgtgtac cttatcagat tcattttcat
    21181 gaatttgatg acaaatcatt aggtaaaata atggaaacag ataagtttga ggtctatgca
    21241 gagcgtctgg cgcataccat tttttgcatg ggttatcgag tagttcaaaa agacttagaa
    21301 ggaaccctag acgcagaagc cttaaaagca gcgggtgttc cttttggacc actctttggc
    21361 aaaattaaaa atggtcaaga cgttgagttg gaagacggcc gcctgatttg tgccaaagat
    21421 tatatttctg cccctaaaaa aggcaaaatc atcaccatta tcggcgatac gcgcaaaacg
    21481 tctgccagtg ttaaactcgc taaagatgca gatgttttgg ttcatgaatc cacctatggt
    21541 aagggtgacg agcggattgc gagaaatcac gggcattcta ccaacatgca ggcagcacaa
    21601 atcgctcatg aagcaggcgc aaaacgcctc ttgctcaatc atgtatcagc tcgcttttta
    21661 gggcgtgatt gtcgccagat ggaaaaagat gccgccacta tatttgaaaa tgttaaaatg
    21721 gtacaagatt tagaggaagt gattatttaa tggcacaaag aatcattgtt atcacgggag
    21781 cttctggagg actggctcag gcaattgtta agcagttacc caaggaagac agcttgattt
    21841 tattaggacg taacaaagaa cgcctagaac actgttatca gcatattgac aacaaagaat
    21901 gccttgagtt ggatattacc aatccagtag ccattgagaa aatggtcgcc cagatttacc
    21961 agcgctatgg ccgtattgat gtcttgatta ataatgctgg ctatggagct ttcaaaggct
    22021 ttgaagagtt ttctgccaaa gaaatagctg atatgtttca ggttaacacc ctagcgagca
    22081 ttcactttgc ttgcttgatt ggtcagaaaa tggcagatca ggggcaaggc catctcatta
    22141 atattgtgtc catggcaggc ttgattgcgt cagccaaatc gagtatttat tcagccacca
    22201 agtttgccct tatcggattt tccaatgccc ttcgcttaga attagcggat aaaggggttt
    22261 acgtgaccac tgtgaatcca ggtcccattg ccaccaagtt ttttgaccaa gctgacccgt
    22321 ctggacatta tttggaaagc gttggtaaat ttactctcca accaaatcaa gtggctaagc
    22381 gtttggtttc tattatcggg aaaaataaac gagaattgaa tttgcccttt agtttagcgg
    22441 tgacccatca attttacacc cttttcccta aattatctga ttatcttgca agaaaggtat
    22501 ttaattataa atgattaaat ccaaatatag ctggaaaata aaagacaaga agccagatga
    22561 tggcttcttt aagctagcaa agacaaaagg tctaacccag accgccgctc agttgattta
    22621 tgatcgaggg ataaggactg aagaagcctt agatgagttt ttaacagcgg acttgtctca
    22681 actgcatgat ccttacttgc tacatgacat gacaaaagct gtgccaagaa ttcgtcaagc
    22741 tattgaagag ggcgaacgaa ttctcattta cggcgactat gatgctgatg gcatgacgtc
    22801 ggcttctatt gtcaaagaaa ccctagacat gatgggagca gagcctttgg tttatcttcc
    22861 caaccgcttt acagatggct atggccccaa ccagagcgtc tataaatatt ttattgagca
    22921 ggaagctgtg tccctaatta tcacggttga taatggcgtg gccggacatg aagcgattag
    22981 atatgctcaa gaacaggaag ttgatgtcat tgtaactgac catcatagcc tgcctgaaga
    23041 attgccagaa gcctttgcca tcattcatcc tgagcatcct gatgctgatt atccctttaa
    23101 acacttagct ggctgtggcg ttgcctttaa attggcaaca gctttgctag aaagtttacc
    23161 aacagactgt ctagacttgg tagctattgg aaccattgcc gatatggtaa gcttgacagg
    23221 tgaaaaccgt gttttagtca aaaatggtct ggccatgctc aaacacaccg aaagagtggg
    23281 cttgcaggag ttgatgtcct tgtctcctat tgatttggaa cattttaatg aagacgccat
    23341 tggctttcag attgctccgc aattaaatgc tttagggcgc ttagatgacc ctaatccagc
    23401 cattgagcta ctgacgggct ttgatgacca agaagctcaa gcgattgcct tgatgattaa
    23461 gaaaaaaaat gaagagcgta aggccttagt ccaagacatt tttgatcaag cgatggctat
    23521 ggttgatcct caaaaacctg ttcaagtctt ggcacaagct ggctggcatc ctggtgttct
    23581 tgggattgtc gctggtcgta tcatggaaac tattggacaa acggtcgtcg ttttgaccat
    23641 tgataatggc tttgcaaaag gctcggccag aagtttagaa gctattaata tttttgaagc
    23701 cttgaatggc aagcgtgagt tatttacagc ctttggggga catgctggtg cagcaggaat
    23761 gaccctgccc gttgacaact tggaagctct ttcagacttt ctttgtcagt ttgtgataga
    23821 acgtggctta gatcagactg ccaaaaatac gttaactatt gatgaaagcc tgtctttgga
    23881 cgaccttagc cttgatattt taaagagttt agataaatta gccccttatg ggatggatca
    23941 tcaaaagccg gtcttttatg tcaaggacat aagggttagt caagcgcgta ccatagggca
    24001 agaccagagc catcttaaat ttaaagtcag tcagggcaaa gcttcctttg acgtactggc
    24061 ttttggtcaa ggaagtcaac tgcaagagtt tcgacaagct actggtttag aattagcagt
    24121 taccttatct gtcaaccatt ggaatgggaa cacgagtctc caattcatgt tagtagatgc
    24181 tcgtgttgac ggagtgcaac ttcttgattt aaggacaaaa acagccaaag ttcctgaagg
    24241 tattccgact attgaagaag acccaaatgc cagagtcatt ctgattaatg acatccctga
    24301 agatttcaaa acctggcgca accagtttgt tcacaaggat tttgacgcca tctattttaa
    24361 aaatcagatg aaacatcctt attatttaac aggatttggc agcagagagc agtttgccaa
    24421 gctctacaaa accatctatc agtttcctga atttgattta cggcataaat tgacagagtt
    24481 gagtcactat ttaaacatag aaaaactttt actgattaag ttgattcaaa tttttgaaga
    24541 gttgtctttt gtgacgattg acgatgggct gatgacggtt aatccccaag cgcaaaaacg
    24601 agagatttca gagagccaca tttaccaaga cttaaaagaa ttggtgaaat ttcaagaaat
    24661 catggctttg gctagcccga aagagatgta tgattatttg gtgaaatcaa acgatcaata
    24721 agtaaagcag ttgacagtag ttattgaggt agttacgaat gaagtatcag ataaacataa
    24781 tgtcagaagt cttattttac gaaaaatatg tttcgtgata taatgaagag tattttatat
    24841 ttggcaattt gccactagaa agaattaact atggatttaa caaattacat tgcatcgatc
    24901 aaagattacc ctaaagctgg tattacgttt cgagatatct cacctttaat ggctgacggt
    24961 aaagcatata gctatgctat tcgtgaaata gcacagtacg cttgtgataa agatattgat
    25021 atggttgttg gacctgaagc gcgtggtttt atcattggtt gtcctgttgc agttgagtta
    25081 ggaattggtt ttgctcccgt tcgtaaacca ggaaaactgc caagagatgt tgtctctgct
    25141 gattacgaaa aagaatatgg tcttgatacc ttaactatgc atgcagatgc tattaagcct
    25201 ggacaacgtg ttttgattgt cgatgattta ctagcgactg gcggtacggt aaaagcaact
    25261 attgaaatga ttgaaaaact tggtggtatt gtagctggtt gtgccttcct tattgaatta
    25321 gaaggtttaa atggtcgcca tgctattagg aactatgact ataaagtctt aatgcagttt
    25381 cctggataag cacattaatc agcatctgat taggggattt aaagtagtaa aggctataga
    25441 ttcgatttta gccttttgat tgagggatta tgagtttttt agaacattat aagtcaggta
    25501 atttggttat cccaagtgcc ttgctttttc attacaaaga tctttttaag agctcagatg
    25561 attttttggt ctggcagttt ttctatcttc aaaatacgac aaagcgagat gacttagcac
    25621 ccagtcaaat tgctcatgcg cttggaaaat cagttgctga tattaataaa atcatttcat
    25681 ctttgactag tcaagggctt ttagatatgc ggacaattga acttactgga gaaattgaaa
    25741 ttatttttga tgcgagtcct gtgttagcca agttagatca attgtttgtc agtcaaacgg
    25801 caactgaaat tgacaaacaa gaaacaccaa accatttcaa acgattagtt gatgaatttg
    25861 aacgtgaatt aggacgattc ttgagtcctt ttgagttaga agatcttgaa aaaacgcttc
    25921 gtgatgataa aacagatcct gatttgatac gagaagcttt aaaagaagct gtcttcaacg
    25981 gcaaaacaaa ttggaaatat atccaggcta tcttacgtaa ttggcgaaaa gaaggtattg
    26041 ttaatcttcg acaagtagaa gaacgtagac gtgttagaga aggcgaagat ctttctcaag
    26101 tgacgatatc agaggatttt ctatctgcga tgaatttatg gagtgattca tgagaattgg
    26161 aaaagcaaga ttagcaaaag tcctgaccat cattggtcaa atgtttcctg aggctaaggg
    26221 agagttagac tgggaaacac cttttcaatt gttgattgcg gtgattttat cagctcaaac
    26281 gacagataag gccgtcaata aggtaactcc tggactatgg cagtcttatc cagaaataga
    26341 agacttagct tttgctgaac tttctgatgt tgaaaatgct ctgagaacga ttggactcta
    26401 taaaaataag gccaaaaata tcattaaaac tgctcaggct attcgtgatg attttaaggg
    26461 tcaagtgcca aaaacccaca aagagcttga aagtttaccg ggtgttggcc gaaaaacagc
    26521 caatgtggta ttggcagagg tttatggtgt tccagctatt gcggttgata ctcatgtggc
    26581 tagagtctca aagaggctca atatttcatc accagatgcc gatgtcaagc agattgaagc
    26641 agatttgatg gctaaaattc caaagaaaga ttggattatt actcaccatc gattgatttt
    26701 ctttggacgc taccattgtt tagccaaaaa accaaaatgt gagatttgtc ccgttcagtc
    26761 ttactgcaag tactatcaaa atacttatgg aaaatcgaaa gcctagcctt tggttttctt
    26821 tttttatgga ggtaattttg ctataataga accattacgt tttagaaaga aatcaagatg
    26881 gatagtcaat tatcaaaccg tttagcccaa gttgcggcct atgttccaaa aggtgtcaaa
    26941 ttattagatg ttggcagtga tcatgcctac cttcccatat ttttggtaga aactaatcag
    27001 atttcagcag ccattgctgg cgaagtggtt aggggtccct acgaatctgc tcttaaaaat
    27061 gtcacacagt ctggccttgc cgagcatatt caggtgcgct tagccaatgg ccttgcagcc
    27121 tttgaagaag ctgatgatgt gacagccatt accatttgtg gcatgggtgg ccgtttgatt
    27181 gctgatattt tagaggcagg taaggaaaaa ttgcagggca ttgaacgtct ggttttacaa
    27241 cccaacaatc gtgaggatga tttacgggct tggttatcgg ttaacgcttt taaaattgtt
    27301 gcagaaacca tcatggctga aaatgacaaa tattatgaaa tcatcgttgc tgagcatggt
    27361 gaaaaggcct tatccgcgac agaattgcgt tttggtccat acctttctca ggaaaaatca
    27421 gtcgttttta aagagaagtg gcaacgagaa atggataagt tagcttatgc cttgtcctgt
    27481 atcccagaag aaaaaactca agagcgtcag cttctcttga caaaaataca acaaataaaa
    27541 gaggtgattg ttcatgaaag ctaaaaccct aattgatgcc tatgaagcct tttgcccact
    27601 tgatttatct atggaaggag acgttaaagg tcttcaaatg ggttcattgg ataaagacat
    27661 tcgtaaggtt atgattactt tagatatccg agagtcaaca gtagcagaag ccatcaaaaa
    27721 tgaagttgat ctcattataa ctaagcatgc tcccatcttt aaaccgctta aagatctggt
    27781 ttcatcccct cagcgtgata tccttctaga tttggtcaag catgacattt ctgtctatgt
    27841 gagccatacc aatattgaca ttgtaccagg tgggctaaat gattggtttt gtgacctgct
    27901 agaaatcaag gaagcaactt acttgtctga aaccaaagaa ggctttggca ttggtcgtat
    27961 tggaacagtt aaagaacaag cgttagaaga gttagcaagt aaggtcaaaa gggtgtttga
    28021 tttagatacc gtgcgactta tccgttacga taaggaaaac cctttgattt caaagattgc
    28081 tatctgtggc ggaagtggtg gtgaatttta tcaggatgct gtgcaaaaag gcgcagacgt
    28141 ttatattaca ggggatatct attaccatac agcacaggag atgctgacag aaggactatt
    28201 tgcggttgac ccggggcatc atattgaggt gctttttacg gagaaattaa aggagaaact
    28261 ccaagggtgg aaagaagaga acggttggga tgtcagcatt atttcaagca aagcctcgac
    28321 caatccattt agccatttat aggctgttaa ggtcttaaaa taagataaaa ggataccagg
    28381 aatgaaaata gccattattg gggcggggat tgtaggatca accgccgctt attatttaca
    28441 acaaagtggt caaaaagaag ttaccatttt tgaccatggt caaggacaag ccactaaggc
    28501 tgctgctggt attattagtc cttggttttc aaagcgtcgc aataaagttt ggtaccgcat
    28561 ggctcgttta ggagcggact tttatcagca attaatcaat gacctcaaag aagatggttt
    28621 tgcaacagat ttttatcagc aaaatggcat ctatgtttta aaaaagcagg aagaaaaact
    28681 aagagatctt tatgaattag cgttagcaag aaaggtagaa tcaccgatta ttggtgagtt
    28741 agccattaaa aatcgtaaag aactggggaa tgattttaaa ggattgattg gctttgataa
    28801 ctgtctgtac gcttcaggag ctgctagagt ggagggagcc gctttatgtg agacgttact
    28861 caaggctagt ggttatccag ttattcgcca aaaagtcact ctgaaacagc aaggaagtgg
    28921 ttatgagatt gctggtcatt attttgatca ggtcattctg gcagcaggag catggctacc
    28981 tgacttattg aggcctttag gctatcaggt tgatgtgcga ccacaaaagg gacaactctt
    29041 ggattatgac gttcatcaca tcatcagcga cacttatcca gttgttatgc cagaaggtga
    29101 aatagatctt attcctttta accaaggtaa gatttctgtt ggtgcgagcc atgaaaatga
    29161 taaaggttat gatttggagc ctgattggca ggtcttagaa aaaattgaaa tgcaagctct
    29221 cacttatttg ccgttattaa aagaagctac tcaaaaatca tgtcgtgtag ggataagagc
    29281 ttataccagt gattattctc ctttttatgg tcaggttcca ggattgaaga acctttatac
    29341 agctagtggt ttagggtcat cgggattaac agttggacct ttgattggct atgaattagc
    29401 tcaattattg ctaggacacg aaggactttt gacgcctagc gactattccc ctgagcctta
    29461 ccttttgcgg tctactggta aagcgtgaca ctgctttcaa aaaatgatag aatgataggg
    29521 tatatagaag aataggagca ctactatgaa aggtattatt cttgcaggag gttcagggac
    29581 acgcttgtat ccactgaccc gtgccgcatc taaacagttg atgcccattt atgacaaacc
    29641 aatgatttat tacccattgt caaccttgat gttggcaggg attaaagata ttttaatcat
    29701 ttcaactccc caagatcttc ctcgtttcga agaattgcta ggagacgggt cagaatttgg
    29761 cattagctta tcctataagg aacaaccaag cccagatggt ttagcacaag ctttcattat
    29821 tggagaggag tttatcggtg atgaccgtgt tgctcttatt ttaggagata atatttacca
    29881 cggtaatggc ctcactaaaa tgcttcaaaa ggcagctgcc aaagaaaaag gagccaccgt
    29941 ttttggttat caagtgaaag accctgagcg ctttggagtg gtggaatttg atgagaacat
    30001 gaatgctatc tctatcgaag aaaaaccaga agtacctaag tcccactttg cagtgactgg
    30061 actttatttc tatgacaatg acgtggtaga gattgctaaa aacattaaac caagcgcgcg
    30121 tggtgagttg gaaattacag acgtcaacaa ggcttacttg gatcgtggtg acctctccgt
    30181 tgaattgatg gggcgtggtt ttgcatggct agacactgga acgcatgaaa gtctacttga
    30241 agcggctcaa tatattgaaa ctgttcaacg cttgcaaaac gctcaagtgg caaatctgga
    30301 agaaattgcc tatcgcatgg gttatatcag taaagaagat gtccataaat tggcgcaatc
    30361 tttaaagaaa aacgaatacg ggcaatactt gcttcgtttg attggagaag cttaatgaca
    30421 gaaacttttt ttgacaaacc attggcttgc cgagaaatca aagaaatccc aggcttacta
    30481 gagtttgata ttcctgttcg tggggataat cgtggctggt ttaaagaaaa tttccaaaaa
    30541 gaaaaaatgt tgccaattgg ctttccagaa cgtttttttg aggaaggaaa actacaaaat
    30601 aacgtttctt tttcacgtca gcatgtgctg cgtggacttc atgctgaacc ttgggataaa
    30661 tacatctcag ttgctgatga tggcaaggtt ttaggagcat gggttgatct tcgtgaggga
    30721 gagacttttg gaaacgtcta ccagacagtg attgatgctt caaaaggaat gtttgttcct
    30781 agaggggttg ctaatggctt tcaagttctt tcagagactg tctcttacag ttatcttgtc
    30841 aatgactact gggctcttga cttgaaacct aagtatgctt ttgtaaacta tgctgaccca
    30901 agtcttggga ttacttggga aaatctagca gctgcagaag tttctgaagc agataaaaac
    30961 catcctcttc ttagtgatgt caaaccactg aaaccaaaag acctataggg aaacaacata
    31021 ctcagatgat tgggattaat catttgaggt ggatatgaga ggagttattc ttctctactt
    31081 caggtaacaa gtaacaacta aaaatgatga aaggaaagtt cagtttctct ctttgagctg
    31141 aacatgggac tcaaatctta gaaagcaatg ttttcttagg cctaaaacgt ctttgttaga
    31201 gtcattactt ttaatagatt ttttcggaaa cgaaaggtct ctttatatct tatgtataaa
    31261 aatattatcg taactggtgg agctggtttc atcggttcta actttgtaca ctatgtctac
    31321 aataaccacc cagatgttca tgtaactgtc cttgataaat tgacatatgc aggtaaccgt
    31381 gctaacattg aagctattct tggtgatcgt gttgagttag ttgttggtga tatcgctgac
    31441 gctgaattgg tagataaatt ggctgccaaa gcggatgcta ttgttcacta tgcggctgag
    31501 agccacaacg ataactcatt ggaagatcca agtccattta tccatacaaa ctttatcgga
    31561 acttacactt tgcttgaagc agctcgtaaa tacgatatcc gtttccacca cgtgtcaact
    31621 gatgaagttt atggagatct tccacttcgt gaagaccttc caggacaggg cgaaggacca
    31681 ggtgaaaaat tcactgctga aacaaaatac aatccatcat caccttactc atcaactaag
    31741 gcagcttctg accttatcgt taaggcatgg gtacgttcct tcggtgtgaa agcgaccatt
    31801 tcgaactgtt caaataacta tggaccatac caacacatcg aaaaattcat tccacgtcaa
    31861 attacaaata tcttggcagg aatcaaacca aaactttatg gtgaaggtaa aaacgtccgt
    31921 gactggattc atactaatga tcattctaca ggagtatggg ctattttgac taagggtcgt
    31981 atcggtgaaa cataccttat tggtgccgac ggcgagaaaa acaacaagga agttcttgaa
    32041 ttgattcttg aaaaaatggg tcaaccaaaa gacgcttatg atcacgtaac tgaccgtgct
    32101 ggtcacgatc ttcgttacgc tattgattct acaaaattgc gtgaagaact cggctgggaa
    32161 ccacaattta caaacttttc agaaggtttg gaagaaacta ttaagtggta cacagaaaat
    32221 gagacatggt ggaaagcaga aaaagatgct gtagaagcca agtatgctaa aactcaagaa
    32281 gtgattaaat aaaacattaa agaaccttgt catatcaacg tttgttgaag atgtcaaggt
    32341 tttttcttgt gttttggggc atttttgggg catattatag ccgatttaag atgtcgacga
    32401 cttcattttt catgcttttc gtgacgtggg tatagatttg ttgagtagtt ttcgagtcag
    32461 catgtccaac tctatccatg atagttttta agggcacttt gttttctgca aggcgactca
    32521 ccagggtatg acggaagatg tggctggtga gttctttttg aatcggtttt tctagtctct
    32581 tatttgccgc tctgatagag gtgttgaggg cgttgtcttg aataggaaca ccatttctgg
    32641 aaatgaagat gtagcccatg tccttgtaat taggattagt atttttttca agagcattaa
    32701 ttttcagtat atcctgaata atttcttttt cacgtttggt tatcaatgtt tcacgccaac
    32761 tggaatttgt tttaggggtg gttttgatag cgtttctata gccatttgag gtgtagtcaa
    32821 gagttccgtg gatttcaatg atatcatttt tgatattgtc tggctgtata gctagagctt
    32881 ctccaatgcg gcagccattt aagctcataa attctgccag ataagccatt ttaatcgatc
    32941 ctttacgtct atacatttca tctaatagcc ttttgagttc atctttttct aaaaacttct
    33001 gggcaacgtc ttcaaagtct ttgatggtct taacaggttt tgcgagttgg gtactaataa
    33061 ctggattgat tgagacatac tctaaagcga tagcgtagtc gaaggtttgt ttgagcagct
    33121 gccgtgctcg tttacgtttg tcataactac ctggtatttt gtcgataata ctttgcaggt
    33181 atttcgtggt tatcttggca atcatcactt ctggatcaat taagtttcgc aattctctta
    33241 ttctaaaatc aagagagcgg acagaagttg atttgagtga tttctgatga tgttcccacc
    33301 attcgttcat gacatctagt aggtaggcat cggttgtggt aagacttgtt aagattttag
    33361 atattttttc gtcaagatat ttttgggctt cttttcgtat tcgtggcgtg tctttttcca
    33421 taaggacaga agctctgcac cacttgccag tatacgggtg tttgtaccgt tctacaaaat
    33481 ttacttttcc gcttttgtgt ttttctgacc acattgtttt ttacctcatt ttctgttaaa
    33541 ataggtatag taaagagacc tactgcgaag caggttttta ctattaccaa cttgacctac
    33601 acgtttgctt gccggcgctg tgtaggtctt tttttaattt gcttttttta tatttattgt
    33661 attttctggg actgctaatc cattagagta gaaaatatac tctattcctt tacttggttt
    33721 tgttactgaa tagttcaggt aatatttttc atacttaaat tctttataca tttgaaaaat
    33781 ttcttcagat aaatcataag tgaga
//
LOCUS       DAUCWO010000012        33728 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUCWO010000012 DAUCWO010000000
VERSION     DAUCWO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33728
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.012"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            210..1058
                     /locus_tag="VGY20_000534"
     CDS             210..1058
                     /locus_tag="VGY20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ4273412.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLYETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            1356..2489
                     /gene="ugpC"
                     /locus_tag="VGY20_000535"
     CDS             1356..2489
                     /gene="ugpC"
                     /locus_tag="VGY20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ4273413.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLDIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            2618..4231
                     /locus_tag="VGY20_000536"
     CDS             2618..4231
                     /locus_tag="VGY20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ4273414.1"
                     /translation="MKKHWWHKATIYQIYPKSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVDDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSKAQETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFATDLAGAQ
                     VIIANTDVDTVLETKHLQPWDAFCLKLKA"
     gene            4429..7926
                     /locus_tag="VGY20_000537"
     CDS             4429..7926
                     /locus_tag="VGY20_000537"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ4273415.1"
                     /translation="MKKKVNQGSKRYQYLLKKRGIGFVIAATGTVMLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSTNPDSIAEHHLRMHFKT
                     LPAGETLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTKVLGQANL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNNATAT
                     DDIKTAKAAFIDPSKLGPAGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLRAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEAPSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            8041..8415
                     /locus_tag="VGY20_000538"
     CDS             8041..8415
                     /locus_tag="VGY20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ4273416.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            8531..8896
                     /gene="yajC"
                     /locus_tag="VGY20_000539"
     CDS             8531..8896
                     /gene="yajC"
                     /locus_tag="VGY20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ4273417.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            9115..9864
                     /locus_tag="VGY20_000540"
     CDS             9115..9864
                     /locus_tag="VGY20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ4273418.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            9877..10671
                     /locus_tag="VGY20_000541"
     CDS             9877..10671
                     /locus_tag="VGY20_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ4273419.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPRVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            10744..12003
                     /gene="rseP"
                     /locus_tag="VGY20_000542"
     CDS             10744..12003
                     /gene="rseP"
                     /locus_tag="VGY20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ4273420.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLYLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            12201..14057
                     /locus_tag="VGY20_000543"
     CDS             12201..14057
                     /locus_tag="VGY20_000543"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ4273421.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSVASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRAVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQAAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            14311..18708
                     /locus_tag="VGY20_000544"
     CDS             14311..18708
                     /locus_tag="VGY20_000544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ4273422.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYENDKLIIAAPRFVNNDHFRKNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVEKAVQDNLEAQKSLEAMMPPVEEATSAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHERKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLARYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGGGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            18818..19246
                     /locus_tag="VGY20_000545"
     CDS             18818..19246
                     /locus_tag="VGY20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4273423.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            19376..19918
                     /locus_tag="VGY20_000546"
     CDS             19376..19918
                     /locus_tag="VGY20_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ4273424.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(19952..20566)
                     /locus_tag="VGY20_000547"
     CDS             complement(19952..20566)
                     /locus_tag="VGY20_000547"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ4273425.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(20667..20936)
                     /locus_tag="VGY20_000548"
     CDS             complement(20667..20936)
                     /locus_tag="VGY20_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4273426.1"
                     /translation="MTQNVCQQNITYIVKERFASYILKAQANQEVHLNFILLANRFET
                     SDRHLKHVLKQPIFQRIIERICFKTYRIADLDALTQLRPLRYAVH"
     gene            21062..21262
                     /locus_tag="VGY20_000549"
     CDS             21062..21262
                     /locus_tag="VGY20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273427.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTAFWLF
                     GLASSVAYGGLVKAKRAHSMTR"
     gene            21438..21707
                     /gene="rpsO"
                     /locus_tag="VGY20_000550"
     CDS             21438..21707
                     /gene="rpsO"
                     /locus_tag="VGY20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ4273428.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            22059..24119
                     /locus_tag="VGY20_000551"
     CDS             22059..24119
                     /locus_tag="VGY20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ4273429.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVISGSWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            24112..24396
                     /locus_tag="VGY20_000552"
     CDS             24112..24396
                     /locus_tag="VGY20_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4273430.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFEDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            24423..25802
                     /locus_tag="VGY20_000553"
     CDS             24423..25802
                     /locus_tag="VGY20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ4273431.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLTIVLVLPSFLSKSQE
                     AINNVSEEI"
     gene            25812..26543
                     /locus_tag="VGY20_000554"
     CDS             25812..26543
                     /locus_tag="VGY20_000554"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ4273432.1"
                     /translation="MMTKKLNIKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTARVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDAKH"
     gene            26823..28955
                     /gene="pnp"
                     /locus_tag="VGY20_000555"
     CDS             26823..28955
                     /gene="pnp"
                     /locus_tag="VGY20_000555"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ4273433.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKV
                     GEVYHAKVIRIEKFGAFVNLFDKTDALVHISEIAWTRTANVSDVLEVGEDIDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            28948..29700
                     /locus_tag="VGY20_000556"
     CDS             28948..29700
                     /locus_tag="VGY20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ4273434.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMFLFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDDNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            29709..30290
                     /gene="cysE"
                     /locus_tag="VGY20_000557"
     CDS             29709..30290
                     /gene="cysE"
                     /locus_tag="VGY20_000557"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ4273435.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            30769..30999
                     /locus_tag="VGY20_000558"
     CDS             30769..30999
                     /locus_tag="VGY20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273436.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            31027..32370
                     /locus_tag="VGY20_000559"
     CDS             31027..32370
                     /locus_tag="VGY20_000559"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ4273437.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLKVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            32363..32776
                     /locus_tag="VGY20_000560"
     CDS             32363..32776
                     /locus_tag="VGY20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ4273438.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            32972..>33728
                     /locus_tag="VGY20_000561"
     CDS             32972..>33728
                     /locus_tag="VGY20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273439.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAM
                     TNSVDVGTKAQTKQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAA"
BASE COUNT         9917 a         6409 c         7589 g         9813 t
ORIGIN      
        1 agaaacaata cctattttag tataaaaata aattaatctt ccgataaata gctatttgat
       61 tgaatgttct tgctaaaaat gaataaccat tatttagctt caatgattag aaagtcagaa
      121 aatacaaaat catgctgaca acaaaccgaa ctgtgtgata aagagtcatg tgttataatg
      181 gtttaaagac cgtcatgaaa ggaatggcta tggagctaaa agactatttt ccagagatgc
      241 aagtaggccc tcatccgtta ggagataaag agtgggtgag tgtcaaagaa ggggaccaat
      301 atgtgcattt tcccaaatca tgtttatccg aaaaagagcg cttattactt gaggtgggac
      361 tgggtcagtg tgaggtcttg cagccgctag ggtcaccttg gcagcggtat ttgcttgatc
      421 atcagggcaa tccccctcag ctgtatgaga cttctcagtt catttacctc aatcatcagc
      481 aggctttacc agctgactta gttgagttgc tccaacagat gattgcaggg cttgaggtca
      541 ttttgcctat cagcgcaact cagacggcat ttttatgccg acaagcaatc tcaataaagg
      601 tcttgcgctg gcttgaagat ctcctgccaa cgctagaaag tgattttggt ttggccttaa
      661 ccatgtttgt tggaaatgcc tggtatcaag tagctgcggg tacattgaga gagtgttttg
      721 aggaggaatg tcagctgtta acggcctatt taaggcaaca atcaggcaga aaattattaa
      781 cattttcagg actgatgctg tggtctttgc tgtcgcacca cacttttttg gcattgacgc
      841 gtcagtttca ccaattccta agtcctcaaa gtgatatggc cgatgtggtt cacgcgctat
      901 ggtcagaaca cggcaatctg gtgcagacag cgcagcgcct ctacattcat cgcaattcgt
      961 tgcaatataa gcttgataaa tttgctcagc aatcaggact acacttgaag caattggatg
     1021 atttagcctt tgctcatctc tttttgttga aatattagga aagccacagg gctttctttt
     1081 ttgctgcctt gactgtagct gataaggagg atgatagatc gttcagcttg ttttggctcc
     1141 tgtgaccggc ctgtgtttac atggttttct tgttttatgg gtctttacga cttgactagc
     1201 cctgctattg tgcaagttgc ccaaagaagt ttggtcgctt tgtctctgtt ttgaaagcgc
     1261 tatttttgtt ataataaaag catcatataa aagatatcag cggtttctag ctttgcagtc
     1321 agctagaacg tttgctattc aaataggagt tacacatggt tgaattaaat ctaaatcaca
     1381 tctataaaaa ataccccaat actactcatt atgccgttga agattttgac ttggacatca
     1441 aagataaaga atttatcgtc tttgttggac cttcaggatg tggtaaatcg actactcttc
     1501 gtatgattgc aggtcttgag gacatttctg aaggagagtt aaaaatcggt ggcgaagtgg
     1561 tgaatgacaa gtctcctaaa gaccgtgata ttgccatggt ctttcaaaac tacgcccttt
     1621 acccacacat gaccgtgtat gataacatgg cctttggttt gaaattacgt aagtacaaaa
     1681 aagatgatat tgaccgtcgt gtcaaggaag ctgcgcagat ccttggtttg accgaatttt
     1741 tagagcgtaa acctgctgac ctgtctggtg gacaacgcca acgtgttgcc atggggcgtg
     1801 ctattgtccg tgatgctaag gtattcttga tggatgaacc tttgtcaaac ttggatgcga
     1861 agcttcgtgt ctccatgcgt gctgaaatcg caaagattca tcgtaggatt ggctcaacaa
     1921 ctatttacgt tacccatgac cagacagaag ccatgacctt ggcagaccgt atcgttatca
     1981 tgagtgccac taaaaaccct caaggtaatg gaacgattgg taaaattgag caagtaggtt
     2041 caccacaaga attgtacaac cttccagcta ataaattcgt tgccggtttt atcggaagcc
     2101 ctgccatgaa cttctttgaa gtggaagtta aagatggccg cattgtgagt gaagatggtc
     2161 ttgacattgc tattcctgaa ggtcaagcaa aaatgcttga agctgctggt tacaagggga
     2221 aaaaagtcac ttttggtatt cgtccagaag acatttctag tagacaaatc gtgcatgata
     2281 cttatccaag tgcaactgtc acagcagaag ttttggtttc tgagttactg ggatctgaga
     2341 cgatgctcta tgtcaagctt ggacaaactg agtttgcttc tcgggtagat gcgcgtgatt
     2401 tccatagtcc aggggagcaa gttagcttga cctttaatgt ggctaagggg catttctttg
     2461 acagagatac cgaacaagct attcgttaag gtataagcga tattcataaa aaggttgggt
     2521 tccagccttt ttatcatgtc atttgcgcaa acgattgcac aaaaggagaa aaaacgttaa
     2581 aataaaagat gaatgaatga ttaataggag aagaataatg aagaaacact ggtggcataa
     2641 agctacgatt tatcagattt acccgaaatc ctttaaagac accaatggca atggtattgg
     2701 tgatttaaaa ggcattacca gtcagctgga ctacttgcaa aaacttggca ttacagccat
     2761 ttggttatct cctgtttacc agtcgcccat ggatgacaat ggctatgaca tctctgacta
     2821 tgaagcgatt gctgatgttt ttggagacat ggcagatatg gatgagctct tagcagcagc
     2881 taatgagcga ggcattaaga ttatcatgga cttggtggtc aaccatacgt ctgatgagca
     2941 tgcttggttt gttgaagcta gggaaaaccc taatagtcct gagcgtgatt tttatatttg
     3001 gcgagatgaa cctaacgatt tgacttctat ttttagtggc tcagcttggg aatatgataa
     3061 tgcatcaggt caatattatt tgcacctttt tagcaaacgt cagccagatc tcaactggga
     3121 gaatgaagct ttacgtcata aaatttacga tatgatgaat ttctggattg ctaaaggcat
     3181 cggtggtttt cgtatggacg tgattgacct gattgggaag gttccagatt ctgaaatcac
     3241 gggtaacggg ccgcgcttac acgattacct caaagaaatg aatcaagcaa cctttggcaa
     3301 ccatgacgtg atgactgtcg gtgagacttg gggagcaacg cctgaaattg ctcgccagta
     3361 ttctcgccct gaaaacaaag agttatccat ggtcttccaa tttgagcatg tgggtcttca
     3421 acataaacct gacgctccca agtgggatta tgccaaagaa ttagacgtgc ctgctctgaa
     3481 agctattttc agcaagtggc agacagaatt aaagttggga gaaggctgga attcgctttt
     3541 ttggaataac catgacttgc cacgggtctt atccatttgg ggcaatgaca gcacttaccg
     3601 agaaaaatca gccaaggctt tagctatttt actgcatttg atgcgaggaa ccccttacat
     3661 ttaccaagga gaagagattg gcatgaccaa ctatcctttt aaagacttaa ctgaggttga
     3721 tgatattgag tcactcaact atgctaagga agctatggga aatggagtgt cagctgcgcg
     3781 tgtgatggat agcatccgta aggtaggacg agacaatgct agaacgccga tgcagtggtc
     3841 taaggacaca catgctggct tttctaaagc ccaagagact tggttgccgg tcaaccccaa
     3901 ctaccaagac atcaatgtgg ccgatgccct tgctgatcca gactctattt tttacaccta
     3961 ccaaaaattg attgccttgc gtaaggagca agactggcta gttgaagcgg attatcacct
     4021 cttgccaaca gctgataaag tgtttgctta tcagcgtcag cttggagaag agacgtatgt
     4081 gattgttgtc aatgtgtctg atgaggagca agtctttgcc acagacttag caggtgcaca
     4141 agtgatcatc gccaatacag atgtggacac ggtattagag accaagcact tacaaccttg
     4201 ggatgccttt tgtctcaaac tgaaggccta gcagttgtat gtgatttgag tttacccaac
     4261 agccaagcga gcggaaactc gttttggctg tttgattctt gagatcctat taaggggtga
     4321 tagtcattgt ttgagctgtg ttctaagttg aagtgcggca cgtcttgcaa gcgcttgcgc
     4381 aatgtgataa gatagtcttg tttgaatgtt atactaaatg gaggtcttat gaaaaagaaa
     4441 gtcaaccaag gatcaaagcg ctatcagtat ctgttaaaaa agcgggggat aggttttgta
     4501 atcgctgcaa ctgggactgt catgttaggg tgcaccccta gtatcttaac acatcaagtt
     4561 gctgctaaaa ccattgttgg actagctcgc gatgaagctc aacaaggaga tggcaatgct
     4621 aaatctggtg atggttttca atcgtctagc aaggaggcaa aaccagtttt agacagctcg
     4681 tcaactaatc ctgatagtat tgctgagcat catttgcgta tgcattttaa aacattgcca
     4741 gctggtgaga cgctaggaag cttgggactt tgggtgtggg gagatgtgga tcaaccttca
     4801 aaggattggc caaatggtgc tatcaccatg acaaaggcga aaaaagatga ctatggctat
     4861 tatctagatg tgccactagc agctaaacac cgccagcaag tgtcttatct cattaataat
     4921 aaagctggag agaatctttc aaaggaccag cacatctcgc ttctcacgcc aaaaatgaat
     4981 gaagtttgga tagacgagaa ttaccatgcg cacgcttatc gacctttgaa aaaaggttac
     5041 cttcgaatca actaccacaa tcaatcggga cactacgata acttagctgt ctggaccttt
     5101 aaagatgtca aaaccccaac gaccgactgg ccaaatggac ttgacttgtc acataaaggg
     5161 ccttatggag cttatgttga tgtcccctta aaagaaggag ctaacgaaat cggattttta
     5221 atccttgata aaagtaagac aggagatgct attaaagtgc aaccaaaaga ttatctgttt
     5281 aaagagttag acaatcatac tcaggttttt gtcaaagaca ctgacccaaa agtttacaac
     5341 aatccttatt atattgatca ggttagtctc aaaggagctg aacaaaccac gccaaatgag
     5401 attaaagcca tttttacgac cttagatggg cttgatgaag atgcggtgaa acaaaacatc
     5461 aagatcactg acaaagcagg gaaaactgtt gcaattgatg agttgacact tgacaaggat
     5521 aagtctgtaa tgacattaaa gggtgatttt aaggcacaag gtgcagtcta cacggttaca
     5581 tttggagaag ttagccaagt cgctcgccaa tcctggcaat taaaagataa actctatgct
     5641 tacgatggtg aacttggagc taccctagct aaggatggtt ctgttgattt agcgctatgg
     5701 tctccaagtg ctgatactgt taaggttgtc gtttatgata aacaagatca gacaaaggtt
     5761 cttggtcaag ctaatttgac caagtcggac aagggtgttt ggagagctca tctgacttct
     5821 gatagtatca agggcattag tgattacaca ggctactatt acctttatga aatcacgcgc
     5881 ggtcaggaaa aagtcatggt tttggatcct tacgccaaat ctctcgctgc ctggaataat
     5941 gcgactgcta ctgatgacat caaaacagca aaagctgcct ttattgatcc aagcaaacta
     6001 ggaccagcag gccttgattt tgccaaaatt aacaacttta aaaagcgtga agacgctatt
     6061 atctatgaag cacatgtgcg agattttacg tcagataagg ctctagaagg caagttaaca
     6121 cacccttttg ggactttttc agctttcgtt gaacagctag actatctcaa agacttgggg
     6181 gttacccacg ttcaattgct accggttttg agttattttt atgccaatga gctggacaag
     6241 agccgctcaa cagcctacac gtcttcagat aataattaca actggggtta tgacccacaa
     6301 cactactttg ccctttctgg catgtattcg gcaaatccta acgaccctgc tttacgtatc
     6361 gcagagctta aaaaccttgt caatgagatt cacaaacgtg gtatgggtgt tatttttgat
     6421 gtggtttata accacacggc tagaacctat ctctttgaag atttggaacc caactactat
     6481 cattttatga atgctgatgg cacagctaga gagagttttg gcggaggtcg tctaggaacg
     6541 acacatgcca tgagccgtcg tatcttggtg gattcgatta cttatctgac tcgtgaattc
     6601 aaggtagatg gttttcgttt cgacatgatg ggtgaccatg atgcggcagc tattgagcaa
     6661 gcctttaagg cagccaaagc cattaatcca aataccatta tgattggcga aggctggcgt
     6721 acctaccaag gtgatgaggg gaaaaaagaa attgcggcag atcaagattg gatgaaagca
     6781 accaatacgg tcggtgtttt ctctgatgat atcagaaata ccctcaaatc aggttttcca
     6841 aatgaaggca cagcagcctt tattactggt ggcgcaaaaa atctagaagg actgttcaaa
     6901 acgatcaaag cacagcctag taattttgaa gcagatgccc ctggagatgt agtgcagtat
     6961 attgcagccc atgacaacct gaccttacat gatgtcattg ccaaatccat caataaggat
     7021 cctaaagtgg ctgaagaaga gattcacaag cgtattcgtc taggaaatac catgatttta
     7081 actgctcaag ggactgcctt tatccattct ggtcaggaat atggacgaac caagcagctt
     7141 ctaaatcccg actacaagac aaaggtgtct gatgacaagg tgccaaataa ggcgactctg
     7201 attgatgctg tagcgcaata cccttacttc atccacgatt cttatgattc gtctgatgcg
     7261 gtcaatcatt ttgactgggc aaaggcaaca gattccatag ctcacccgat tagcaaccaa
     7321 acaaaagcct atacacaggg actaattgcg ttgcgtcgct caacagatgc ctttacaaaa
     7381 gcaaccaaag ctgaggtaga tcgggatgtg accttgatca cccaagcagg acaagatggt
     7441 attcaacaag aggacctcat catgggttac caaacggtgg catcaaatgg agatcgctat
     7501 gctgtctttg tcaatgcaga caacaagacc cgcaaggtag ttttacctca agcctaccgc
     7561 tatttgctaa gagcccaagt gcttgctgat gctgagcaag ctggtgttac tgccattgct
     7621 aagcctaagg gagtccagtt taccaaagaa ggcttgacta ttgatggcct aactgccctg
     7681 gtcctcaaag tatcctcaaa aacggctaat ccctctcagc aaaagagtca gacagataat
     7741 catcaaacca aaacaccaga tggctcaaaa gacctagaca aatcattaat gactaggcca
     7801 aaaagagcta aaacaaacca aaagctccca aaaacgggtg aagccccctc aaaaggctta
     7861 ttagcagccg gagtagctct gcttttattg gctattagcc tgttgatgaa gcgccaaaaa
     7921 gattagggtg gtgttaatag agacaaaaag attgccgttt gcggattctt tttgacggct
     7981 ataaaagctc tgagctgtga aaagaccttg tgattgagaa aattgctact ttagtgtatc
     8041 atgagaccaa aagagaagga gacatccatg acatttgaag aaatcgttgc gaattttata
     8101 ccgtcgagtg ttgcagaagt cacatcagct atcgctagtg gcaaagacat gattgtcttt
     8161 ttaggacgtt cgagctgtcc ttattgtcgc cgctttgctc ccaagttggc acaagtagca
     8221 acagataatc aaaaagaagt ctattttgtg gatagcgaaa acgctgctga tgctgctgag
     8281 cttgctgcct ttcgtgagaa ctaccaatta gtgacagttc cagctttgct ggttagttac
     8341 gatcagcacc aaagggctgt atgtgattca agtttgacac cagatgacat cttagctttt
     8401 ttgactcgtg agtaaagcac tgcgcttagc gtctcgttta aagaagacat caggtaacgt
     8461 ctagtcttgc tataccgctt gatttttgat atagtaagac atgactttta tagaataaaa
     8521 aggagacatt atgggatttc caacaatttt aatgtttgta gtgatgcttg gattgatttg
     8581 gtttatgcaa cgtcaacaaa aaaaacaagc gcaagaacgc caaaatcagt taaacgctat
     8641 tgaaaaagga gacgaagtcg taacgattgg tggcatgttt gcgattgttg acgaagtcga
     8701 tacaacagct aaaaagattg tgttagatgt ggatggcgtc tttttgacat ttgagctatc
     8761 agctattaag cgtattgtaa caaaagctac cacagaaacg acactggttg aaactgaaga
     8821 aaccagtgtt gttaatgagc ctgctgttga gacagtagat agcactgaca ctgacagtgc
     8881 cattgaaagc cattaaggcg gggaaagttt ggatgaaata tgccaaactt ttttgttttg
     8941 atccctggac tgaaacagtt attctgatgc taacaggttc atgaaaagct gacacatcta
     9001 tcagaggata ggtgaacatt ttgtgaagat aagatctaat tgataaaaaa caagccaaaa
     9061 ggcgtgaaat atgataaaat atgagataag tatgcttatc aataaggaaa aaacatgttt
     9121 ggattaaaag cgaaatcaac aaaaaaagtt ttggggagta tcccaaagca tattggaatt
     9181 atcatggatg gtaatggacg ttgggccaaa aaaagactaa aacctagagt gtttgggcat
     9241 aaagctggga tggatgcttt acaagaagta acgattactg catctgagtt gggggttaaa
     9301 gttctgacag tctatgcctt ttcgacagaa aactggtcca gaccccaaga tgaggtgtct
     9361 tttatcatga atttaccagt aaccttcttt gacaaatatg tgcctgtgct tcatgagaac
     9421 aatgtaaaga ttcaaatgat tggagagact agtcgtttac ctgaagatac cttggcagcg
     9481 ctaaatgcag ccatcgacaa aacaaaacgt aatacgggtt taattttgaa ttttgctctc
     9541 aattatggag gacgtgctga gattaccagt gctgttcgct ttattgctca agatgtctta
     9601 gatgccaaat taaatccagg agatattacg gaggatttga ttgctaatta cttgatgaca
     9661 gaccatctgc catatctcta ccgagacccc gatttgatta ttcgcaccag tggtgagctg
     9721 cgtttgagca atttcttgcc ttggcaatca gcttacagtg aattttattt cacaccagtc
     9781 ttgtggcctg attttaaaaa ggcagagcta ctaaaggcca ttgcggatta taaccgccgc
     9841 caacgtcgtt ttggcaaggt ctaaaaggag atagttatga aagagcgtgt tgtttggggg
     9901 ggagtagcag tagctatttt cctacctttt ctcataatag gaaacctacc attccaatta
     9961 tttgtaggcg ttttggccat gattggggta tcagaattac tgaaaatgaa aagattagaa
    10021 gtcttttctt ttgaaggtgt ttttgccatg ttagccgcct ttgtattggc agttccgatg
    10081 gaccattacc tcactttttt accaattgat gcgaatgttg ctttttacag cttaatggtt
    10141 tttttcattt tggcaggtac tgttttaaat agcagagctt attcatttga tgatgcggcc
    10201 tttccgattg caactagttt ttatgttggg attggttttc aacatttgat caatgctcgc
    10261 ctgtcaggaa ttgacaaggt ctttttggcc ttatttatcg tttgggccac tgatattgga
    10321 gcatatctga ttgggcgtca gtttggtaga cgcaaactat tgcctagagt ttctccaaac
    10381 aaaaccattg aaggtagttt aggagggatc gcttgtgcag tccttgtgtc ttttatcttt
    10441 atggtgattg ataggtctgt ttatgcgcca catcactttt tgaccatgtt ggtattggtc
    10501 gctctttttt ccatttttgc ccaatttggt gatttggttg aaagtgctct caaacgtcac
    10561 tttggtgtca aagattcagg taagcttatc ccgggacatg gtggtatttt agaccgcttt
    10621 gattccatga tttttgtttt tcccatcatg catctctttg gtttatttta atcttaagag
    10681 gtaatgtttg gagtcgcaac aacgcgacaa aactcaaaaa agcaatagac tgaaaggaaa
    10741 tctatgttag gaataataac ctttattatt atttttggta ttttagtgat tgtccatgaa
    10801 tttggacatt tctattttgc taaaaaatca ggcattctag taagagaatt tgccattgga
    10861 atgggcccta aaattttttc tcatgttgac caaggaggaa ctctttatac cttgaggatg
    10921 ttacccttgg gcggttatgt gcgaatggct ggttggggtg atgacaaaac tgagatcaaa
    10981 acaggtactc cagcaagttt aacccttaat gagcaaggtt ttgttaagcg catcaacttg
    11041 tctcaaagta agttagaccc aacgagtctc ccaatgcatg ttacaggcta tgacttagaa
    11101 gatcagctga gtattactgg cttggtttta gaagaaacca agacatataa ggtcgctcac
    11161 gatgctacta ttgttgaaga agacggtact gagataagaa ttgctccgct tgatgttcaa
    11221 tatcaaaatg ctagtattgg cggacgttta atcaccaatt ttgcaggccc catgaataat
    11281 tttattttag ggattgtggt ttttatcctc ttggtctttt tacaaggtgg gatgccagat
    11341 tttagtagca atcatgtccg tgttcaagaa aatggagcag cagctaaggc tggtcttcga
    11401 gataatgacc aaattgtcgc aattaatggt tataaggtga ctagctggaa tgatctcact
    11461 gaggctgtag accttgcaac acgggatcta ggcccgtcac agaccattaa ggtcacctac
    11521 aagtcacatc agcgcttaaa aacggtggct gtgaaaccac aaaagcatgc aaagacatac
    11581 acgataggag ttaaggcgag tctgaaaaca ggatttaagg ataagctctt aggcggtcta
    11641 gaattagctt ggagtggggc gtttactatt ttgaatgctt tgaaaggatt gatcactggc
    11701 tttagtctca ataaattagg tggacctgtt gccatgtatg acatgtccaa tcaggctgct
    11761 caaaacggct tagagtcagt cttatatctt atggcaatgc tttcgatcaa tttagggatc
    11821 tttaacctga ttccgattcc tgcacttgat gggggaaaaa tcttgatgaa tatcattgaa
    11881 gccattcgtc gcaagcctat caagcaagaa acagaggcct atatcaccct agctggggtt
    11941 gctatcatgg tcgtattgat gattgctgtg acatggaatg atatcatgcg cgtctttttc
    12001 taatcaagat aggtcatctt aagctgacct tatcatgtta aagatcgaaa aagacctcaa
    12061 ccctaagatt tgcaaggatt gaggtcttac tcaaaatgaa aggaatacac attcaaatta
    12121 ttgaataatc ccgcttcact cttacttgat gtttccagaa gggtttgagg ataaggggat
    12181 gtattaaaac ggaataatct atgaaacaaa gtaaactgct tatcccaacc ttgcgcgaaa
    12241 tgccaagtga tgcccaggtt atcagccacg cgcttatggt gcgtgccggt tatgtgcgcc
    12301 aagtttctgc tggtatctat gcttatttac cactggcaaa tcgtaccatt gagaaattca
    12361 agaccatcat gcgtgaagag tttgaaaaga tcggtgctgt tgaaatgttg gcaccagctc
    12421 ttttgacagc tgatctctgg cgtgaatcag gccgttatga gacctatgga gaggacctct
    12481 ataagcttaa aaaccgtgat aactcagact ttatcttggg tccgacccac gaagaaacct
    12541 ttacgacttt ggtgcgtgat gcggtcaaat cttacaagca attgccctta aacctttatc
    12601 aaattcagtc taagtatcgt gatgaaaaac gtccgcgtaa tggcttgctt cgtacccgtg
    12661 agtttatcat gaaagacggc tatagtttcc atcacaacta tgaagattta gatgtgacct
    12721 acgaagatta tcgtcaagct tacgaagcta tttttaccag agctggtcta gatttcaaag
    12781 ggattattgg agatggcggt gccatggggg ggaaagattc ccaagaattt atggccatta
    12841 caccagctcg tacggacctt gatcgctggg tggttcttga caagtctgtt gcgtcaatgg
    12901 atgatattcc aaaagaggtc ttagaagaga ttaaggcaga attggctgct tggatgattt
    12961 caggtgaaga tactattgct tattcaacag aatcaagcta cgctgccaac cttgagatgg
    13021 caactaacga atacaaaccg tcctcaaaag tagctgctga agatgccttg gcagaagttg
    13081 agacaccaca ttgcaaaacg attgatgaag tggctgcttt tctttcagta gatgaaacac
    13141 aaaccatcaa aaccttgctt tttgtggcag ataatgaacc tgttgttgct ttgctagttg
    13201 gaaatgacca tatcaatacc gttaaattaa aaaactatct agctgctgat tttttagagc
    13261 cagctagtga agaagaagcc cgtgctttct ttggtgcagg ttttggctca cttgggcctg
    13321 tcaacttggc acaaggtagt cgcattgtgg ctgaccgcaa agtgcaaaac cttaccaatg
    13381 ccgttgcagg agctaacaag gatggtttcc atgtgacagg agtcaatcca ggacgtgact
    13441 tccaggctga atatgtagat attcgtgaag tcaaagaagg ggaaatgtct ccggatggtc
    13501 atggtgttct ccagtttgcg cgtggtatcg aagtcggtca tatcttcaag ttgggcactc
    13561 gttattcaga cagcatggga gcaacgattc ttgatgaaaa tggcagagcc gttccaattg
    13621 tcatgggatg ctatggtatc ggtgttagcc gtatcctgtc tgctgtcatt gaacagcatg
    13681 cccgtctctt tgtgaacaag acaccaaaag gtgattaccg ttatgcttgg gggattaact
    13741 tccctaaaga attggcaccg tttgacgtgc atttgattac ggttaacgtc aaagaccaag
    13801 cagcacaaga cctaacggcg aagttagaag ctgacttgat ggctaaaggg tatgatgttt
    13861 tgacagatga ccgtaatgaa cgcgtcggtt cgaaattctc tgatagcgat ttgattggtt
    13921 tgccaattcg tgtcactgtt ggtaaaaaag ccgctgaagg tatcgtggaa attaaaatca
    13981 aggcaacagg tgacagcatt gaagttaatg cagaaaacct catcgaaacc cttgaaattt
    14041 taacaaaaga acactaaaaa agaaatgaag aggctgcctg tttagagcgg cctctttttg
    14101 ctgtgaactt gtctgataca gagtagcttt tgctttacca aaaacgcttg gcgataaaag
    14161 attgacaaaa aaatgatctc agctgagcta tcacttggac taaattagcc aaagaacgtc
    14221 agaagaaagc cctgcttttc tttccatcta aaactcgaaa atcatcttga attgtgataa
    14281 aataaagcct agtaaaatca ggagaaaatt atgtcagatt tattcgctaa attgatggac
    14341 cagatagaaa tgccacttga catgagacgt tcaagtgcct tttcatctgc tgatattatc
    14401 gaggtaaagg tgcattcggt gtcacgcttg tgggaatttc attttgcttt cgcggcggtt
    14461 ttaccgattg caacttatcg tgaattgcat gatcgtttga taagaacttt tgaggcggct
    14521 gacattaagg taacctttga tatccaagct gctcaggtgg attattcaga tgatctgctt
    14581 caagcttatt accaagaagc ttttgagcat gcaccgtgta atagtgctag ttttaaatct
    14641 tctttctcaa agctcaaagt gacttatgag aatgacaaac tcattattgc agcgccgcgt
    14701 tttgttaata atgaccattt tagaaaaaat catttgccta atttggtcaa gcaattagaa
    14761 gcctttggct ttggcacctt aaccattgat atggtgtcag atcaggagat gactgagcat
    14821 ttgaccaagg attttgtttc cagtcgtcag gctcttgtgg aaaaggctgt gcaggataat
    14881 ttggaagccc aaaaatccct tgaggccatg atgccaccag ttgaggaagc cacatctgct
    14941 cctaagtttg actacaagga acgagcagct aagcgtcagg cagggtttga aaaagcgacc
    15001 atcacaccaa tgattgagat tgagaccgaa gaaaaccgga ttgtctttga ggggatggtt
    15061 tttgatgtgg agcgtaaaac gactagaaca ggtcgtcata tcattaactt taaaatgaca
    15121 gactatacct cctcgtttgc tctccaaaaa tgggccaaag acgatgagga gctccgtaaa
    15181 tttgatatga ttgctaaggg ggcttggtta cgggtacaag ggaatattga gaccaatcct
    15241 tttacgaaga gtctcaccat gaatgtccag caggtcaaag aaattgtaca tcatgagcgc
    15301 aaagacctga tgccagaagg gcaaaagcgg gtcgaacttc atgcccacac caatatgtct
    15361 accatggatg ccttaccgac agtagaaagc ttgattgata cggcagccaa gtggggacac
    15421 aaggcggttg ctatcaccga ccatgctaat gtgcaaagtt ttcctcatgg ctaccatagg
    15481 gctcgcaaag ctggaattaa ggctattttt ggcctagaag ccaatattgt tgaggacaag
    15541 gtgcctattt cttatgatcc tgttgatatg gatttgcacg aagctaccta tgtggtcttt
    15601 gacgtggaaa ccacaggtct atctgctatg aataatgacc tgattcagat tgcggcttcc
    15661 aaaatgttta aaggaaatat tgtagagcag tttgatgaat tcattgatcc tgggcatcct
    15721 ctttctgcct ttaccaccga attgacaggg attaccgata agcatttgca gggcgccaag
    15781 ccattggtta ccgtcctaaa agcttttcag gacttttgca aagatagtat cttggttgcc
    15841 cacaacgcca gttttgacgt gggctttatg aacgccaatt atgaacgcca cgacttgccc
    15901 aaaatcacac agcctgtgat tgatacctta gaatttgcta gaaacttgta tcctgaatac
    15961 aagcgtcacg gtttgggacc gctcaccaag cgtttccaag tgagtctaga ccaccatcat
    16021 atggccaatt acgacgcgga agccacagga cgtcttttgt ttatttttct aagagatgcc
    16081 agagaaaagc atggcatcaa aaatcttttg caactcaata cagatttggt ggctgaggat
    16141 tcttacaaaa aagcgcggat taagcatgcg actatctatg tgcaaaatca ggttggtctt
    16201 aaaaatatgt ttaagttggt cagcctttcc aatatcaaat attttgaagg ggtgccgcgt
    16261 attccaagaa ccgtcttaga tgctcacaga gagggtttgt tactaggtac agcttgttct
    16321 gacggcgagg tttttgatgc cgttctgact aaaggaattg atgcggcggt tgatttagct
    16381 aggtattatg attttatcga aatcatgcca ccagccattt accagccatt ggttgtccgt
    16441 gaattaatca aagatcaagc aggtattgag caggtgattc gtgacctcat tgaagtaggg
    16501 aaacgagcta agaaacctgt gcttgccact gggaatgtgc attatctaga gcctgaagaa
    16561 gagatttacc gtgaaattat tgtgcgtagt ctcggtcagg gtgccatgat taatagaaca
    16621 atcggccgtg ggggaggggc acagcctgct cctctaccta aggcgcactt tagaacaacc
    16681 aatgaaatgc tggatgagtt tgcctttctt ggaaaagacc tcgcttatca agtggttgtg
    16741 caaaatactc aggattttgc ggaccgtatt gaggaagtgg aagtggttaa gggcgatctt
    16801 tacactccgt atattgacaa ggccgaagag acggttgccg aattaaccta tcaaaaagcc
    16861 tttgaaattt atggtaatcc tctaccagat attatcgatt tgcgaattga aaaagagcta
    16921 acctctatct tggggaacgg ctttgccgtg atttacctag cgtcacaaat gcttgttaac
    16981 cggtcaaatg agcgaggcta tctggttggt tctaggggat ctgttgggtc cagttttgtg
    17041 gcaaccatga tcgggattac tgaggttaat cctatgccgc ctcactacgt ttgcccgtcc
    17101 tgccaacatt ctgaatttat cacagatggt tcagtcggat cgggctatga tttacctaat
    17161 aagccttgtc caaaatgtgg caccccttat caaaaagacg gccaagatat tccctttgag
    17221 accttccttg gatttgacgg ggataaggtg cccgatattg atttgaactt ctctggtgat
    17281 gaccaaccca gtgcccattt ggatgtccga gatatttttg gtgacgaata cgcctttcgt
    17341 gctggaaccg taggtaccgt ggcagaaaaa acagcttatg gatttgttaa aggctatgaa
    17401 cgcgactatg gcaagttcta tcgggatgct gaggtggatc gtctagcagc aggtgctgct
    17461 ggtgtgaaac gaacgactgg gcagcaccct ggggggattg ttgttattcc taattacatg
    17521 gatgtttatg attttacccc cgtgcaatat ccagccgatg atgtaacggc ttcttggcag
    17581 acaactcact ttaacttcca tgatattgat gaaaacgtct tgaaacttga tatcctaggg
    17641 catgatgatc cgaccatgat tcgtaaactt caggatttat cgggcattga tcctattact
    17701 attcctgctg atgatccggg ggttatggct ctcttttctg ggacagagat tttgggcgtt
    17761 accccggaac aaattgggac accgactggt atgctaggca ttccagaatt tggaaccaac
    17821 tttgttcgcg gcatggttaa tgagacgcat ccgaccactt ttgcagaatt gttgcagttg
    17881 tctggactat ctcatggaac cgatgtttgg cttggcaatg cacaagattt gattaaagaa
    17941 ggcattgcga ccctaaaaac cgttatcggt tgtcgtgacg acatcatggt ttacctcatg
    18001 cacgctggct tgcaaccgaa aatggccttt accattatgg agcgtgtgcg taagggatta
    18061 tggctaaaaa tttctgagga agaacgtaat ggctatattg atgccatgcg agaaaacaat
    18121 gtgcccgact ggtacattga atcgtgtgga aaaatcaagt acatgttccc taaagcccat
    18181 gcggcagctt atgtcttgat ggcccttcgg gtggcttatt tcaaggtgca ctatcccatt
    18241 atgtattatt gtgcttattt ctctattcgt gcgaaggctt ttgaattaaa aaccatgagt
    18301 ggtggcttag atgctgttaa agcaagaatg gaagatatta ctataaaacg taaaaataat
    18361 gaagccacca atgtggaaaa tgacctcttt acaaccttgg agattgtcaa cgaaatgtta
    18421 gaacgcggct ttaagtttgg caaattagac ctttacaaaa gtgatgctat agaattccaa
    18481 atcaaaggag atacccttat ccctccattt atagcgttag aaggtctggg tgaaaacgtg
    18541 gccaagcaaa tcgttaaagc tcgtcaagaa ggcgaattcc tctctaaaat ggaattgcgt
    18601 aaacgaggcg gggcatcgtc aacgctcgtt gagaaaatgg atgagatggg tattttagga
    18661 aatatgccag aagataatca attaagtctt tttgatgact ttttctagat tatcatgatg
    18721 gtagtcatat gcaaagtcat aaaagaagag ggacaagatc actttctaat ctctctgata
    18781 ctaaactgtg acataccgta taaagcgagg aaatcaaatg agtcatttag ataagaacac
    18841 cgctttgaaa gccatggtgg tgtttcgtaa agctcagcga acgctagatg cttttggggc
    18901 tgatattttt aaaaaggcag atttaacagc cacgcagttc agtgtgcttg aagtactata
    18961 tactaaaggc tgtatgcgaa tcaatcactt gattgattct ttgttagcga cttcggggaa
    19021 tatgactgtt gtactaaata atatggaacg taatggctgg attagcaagt gcaaagacaa
    19081 aacggataaa agagcctatg tggttacctt aacagataag ggtactcgct tgattgaagc
    19141 tgttttgcct aaacacgtcg cacgggtgga agaagccttt gctgttttga ctgaaaaaga
    19201 acaactttgt ctgattgaac ttctcaaaaa gttcaaacaa ttataaacaa atctctttac
    19261 ggctcttctt agagaagata taattactac atagtgataa atttcaaaaa gaagtatcaa
    19321 tgaaatacca cttttagaaa gctttaaaac gatctttaaa aaagaggaca aagagatgaa
    19381 acatatttta tttattgttg gctcgcttcg tgaagggtct tttaaccatc aattagcggc
    19441 tcaagcacaa aaagctctgg aacatcaagc agttgtatct tacttaaatt ggaaagacgt
    19501 tcctgttttg aatcaagata tcgaagctaa tgcaccttta ccagttgttg acgctcgtca
    19561 agctgttcag tcagcggatg ctatctggat ttttacacca gtttacaact tctctattcc
    19621 aggttctgtt aaaaacctgc tagactggtt gtctcgtgct cttgatttgt ctgatccgac
    19681 gggcccatct gctattggcg gtaaggtggt tacggtctct tcagttgcaa atggcgggca
    19741 tgatcaagta tttgatcagt ttaaagcact attgccgttt atccgaactt cagtagcagg
    19801 agagtttaca aaagcaactg tgaatcctga tgcctgggga acaggaaggc ttgagatttc
    19861 aaaagagaca aaagcaaact tgctatctca ggcagaggct cttttagcgg ctatttagac
    19921 taacaaaaaa aggctaaagg gccttttttt gttagtctaa aatcaagagc tcttctttgg
    19981 tttcaaaggg gtttttagca ttgatccgat cgtagaacaa aacaccattg atatggtcaa
    20041 tttcatgttg aaccacaata gcgttgtatc cctttaattt gatgcgatgc tgttgtcctt
    20101 ctttatcata gtagtctact gtaacacgag catggcgaac cacataccct tcaacaacac
    20161 gatcgacaga taaacaacct tcaccatcag aaagggctgc atcttgaaca gaatgagaaa
    20221 cgattttagg attatacagg acttcttgcc agctataggc ttctttagga ggatttcctt
    20281 ctttatcagg aagattagga acgagcacag cgatgatacg ttttgacaca tcaatctgag
    20341 gagctgctag accaacaccg gccctaagac caagtttttc cgccattaca ggatcttgag
    20401 aatgcttcaa aaattgcatc atcttttctc ctaataggat atcctcatca cacaagggta
    20461 agctcacttc tttggcgaca gctcttaaag ttggattccc ttcacgaata atgtcatcca
    20521 tggtaatcag atgactaggt ttaattaatt tatcttgtgc agacatgtct actcctttac
    20581 ttttttttaa ctggatttac tatatcacaa atcatttctt ttttaaagaa aagtcagata
    20641 gtggttagag tgattgttat tataagttaa tggactgcat acctcagagg tcttaattga
    20701 gttagagcgt ctaaatcggc aatacgatag gttttgaagc agatgcgttc gataatcctc
    20761 tgaaaaatgg gctgcttaag tacgtgctta agatggcgat cacttgtttc aaaacggttt
    20821 gctaataata taaagttcag atgaacctct tggttggctt gcgctttaag gatataggaa
    20881 gcaaagcgct ctttgacgat ataggtaatg ttttgttggc aaacattttg tgtcaactct
    20941 tgacctatgt cataaaggca ggtagcatcg gttaataagc tgtgttcatg gattagggat
    21001 tgggtttgtt aatactagag caattcatat tatcagtgaa cactttattg gaaagaaatg
    21061 aatgactatg ctaggctacc gcttatcagc tgaagtgctg ggatcagcca tcttacctct
    21121 tttagctggt tatcttatta ggcaagcaat cgcacaggtt tattctgatt taaaaacagc
    21181 cttttggcta tttggccttg ctagttctgt tgcttatggt ggtttggtta aagcaaaaag
    21241 agcacattca atgaccagat aaccttttta agcctagtga ggtggtttgg atgtgagagc
    21301 tcagaataat aggataacgg ttgacctaat attatgaatc tgctagaata gagtggtcga
    21361 aagacaaacc aactttttct tggttttagg agtgccaatc ctaggactcg gattaagcaa
    21421 aaaaaggaga atatattatg gcaatctcaa aagagaaaaa aaatgaaatc attgctcaat
    21481 atgcacgtca cgaaggcgat acaggttcag ttgaagttca agtagcagtt cttacatggg
    21541 aaatcaacca cttgaacagc cacatcaaag aacacaaaaa agaccacgct acttaccgtg
    21601 gattgatgaa aaaaattggt caccgtcgta acttgttggc atacctacgt cgtacagatg
    21661 ttaaccgtta ccgtgagttg atccaatctc ttggacttcg tcgttaattt caagtttaaa
    21721 aagtcctctg cttatgcagt gggctttttc atttttgaca tgttgtttgt taacttacac
    21781 atttggctag aatggtctaa gactgttttt ttagcagttg atgattttat ttagcaacaa
    21841 aaggtatctc agtgatgagg tatcttttgt tgtatcatga agaaaaaggg aatcaattcc
    21901 cagatttgcc tattttttgc caccttttga ggaacttgtt acaatgatat cacctgttga
    21961 gtccaattgc agttaaaaat gacgagagac aattggactc aatttgtctt gaaaatgaaa
    22021 acgcttcatt ttatactgac attataggag gagatgacat gctatctcac gagctcatca
    22081 gaaactatca actcttttct aaatataaag gacattcact ggaggcattt gaatccatat
    22141 taaagacaag caaacgtcat atactggcag atattgctaa aatcaatgac acgttgtcac
    22201 tctatcagtt accccttatt gctctggaca ggcagctggt ttatccgcca gaccttactg
    22261 aaaaggactt gttgaatcgc atgctaccta ccttagacga ctatctcttt caagatgagc
    22321 gtctggacat gattatcatt tacatcatga tggctaaaga atttatctcc attaaccact
    22381 tggaaagctt gttacgccta agcagaaatt ctgttattgc tgatttaaac ttggtgcgtg
    22441 atcgggtaca agcctttcag gtaactttag cttacaatcg ccaggatggt tatttttttg
    22501 aaggagaacc cttagccttg cggcgtctct tagaatcagc ggtcagttct cttttgcaag
    22561 tcatatcagg atcttgggtg ttcagttact tattgcatga acttggtttg cccgaccaga
    22621 caaaggtgat ggcagcaacg ttggaggaac tgagcaggga gaaccactta acttttattt
    22681 cggagaaatt gagggatttg atttattttt tctgccttct cgctcatcga ccattttcac
    22741 ggaatgtaag agctgaagca gtggatactt ttcctttagc ttcgccagct gttgaaacga
    22801 tggtggacca gttattggtc aacttcccca gtctaaccga agaaaaatat ttggttcaat
    22861 ctaggttact tggttgtatc caaggtgact tagagttagt ctttcagcaa cctatttatg
    22921 acattatgga ggaaatcatc aattcggtgg cggttaacac gggattgtct atcaccgata
    22981 ccccagaact tcgtcagaac ctatacagtc accttttgcc agcttattac cgactttact
    23041 acgacattaa cttgaccaat cctttgaagg aacaaatcaa acaagattat gaatctctct
    23101 tttacttggt caagcgcagc ctttctcctc tagaaaaaca attggggaaa tcagttaatg
    23161 aagatgaggt tgcttatttt accattcatt ttgggagatg gttgcaggcc cctaagaaga
    23221 ggccaagcaa tcagctggta gccttatctg tttgccctaa tggcattagt tcgtctctga
    23281 tgttggaggc aaccttgaag gaactttttc cacagctaca gtttattagg attcaccagc
    23341 tggacaaaat aaagttgttg gatccagctt cctttgattt gattttttca acagtagctt
    23401 ttgactgtgc taaacctgtt tatgtgacgc aagctttgat gggacctgtt gagaaaatga
    23461 tgttgaaaaa gatggtctgt gatgattttc atcttccctt gtcagagcaa ttcgctttgg
    23521 atgacctgtt gagtattatt cataaacaca ccacgattac taataaagaa ggacttgtta
    23581 gtgatttatc ccgttacctg attggtaacc atttaacgat tgaaaaagga ggtctaggac
    23641 tattggactt gttaacagca gattttatta ggcaggctga tgctgtttcg gattggcagg
    23701 aagcgattcg tttggctgct cagcctttac tagaacacca gatgattgaa acatcttata
    23761 tcgatggcat gattgattcg gtcaatgagc ttggggccta tattgtttta gcccctaagg
    23821 tggctgttcc tcatgcggcg cctgaaaaag gaacgcggca gttaggcatg tctctcttac
    23881 aactaaaaga acctgtcagc tttgatttga agcaagaagg tgatccagac aagcaagtgc
    23941 aattgatttt tgttttgtct gctgtggatt ctagttcaca tttgaaggct ttgcaggaac
    24001 tgtcgttgat tttagatgat gatgaacata ttgagcaatt aattgaggct aagaatactg
    24061 aggaaataat gagtctgatt agccatatga ttgaaaaagg agatgaatca catgattaaa
    24121 attgtaacgg tttgtggaaa cggtattggt agtagcttgt tacttcgcat gaaagtagaa
    24181 gctatcgcct ctagtttggg tattgatgtg gatgcagaat cctgtgattc caatgcggct
    24241 gttggaaagg gtgcagattt gtttgttacc gttaaagaat ttgaagatat ttttccagag
    24301 gatgccaagg tttgtatcgt taaaagctat accaatcgta aaaaaattga agaagatttg
    24361 gttccggttc tcaaggaaat gagtggcaag gaataagcct ttaaaggaga taggaagata
    24421 ctatggaagc attattatca tttattcgag atattttaaa agaacctgca tttttaatgg
    24481 gcttgattgc ctttgcaggg ttggtggctt tgaaaacacc tgctcataag gtgttgacag
    24541 gaaccttggg gccgattttg ggatacctta tgcttgctgc aggggcgggt gttattgtga
    24601 ccaatctgga ccctcttgcc aaactcattg agcacggttt tagcatcact ggcgtagtgc
    24661 caaataatga agccgtcact tctgtcgctc aaaagattct gggtgtggaa accatgtcca
    24721 tcttggtggt tgggttattg ctcaatttgg cttttgctcg ttttacccgc ttcaaataca
    24781 ttttcttaac aggacatcac agcttcttta tggcttgtct cttgtccgcc gtgcttggag
    24841 ctgttggttt caaaggaagt cttttgatta tcttaggtgg gttcctcttg ggagcttggt
    24901 cagctatttc gccagccatt ggtcaacagt atactctgaa agtgactgac ggagatgaaa
    24961 tcgccatggg acactttggt agtttgggct attacctttc tgcctgggtt ggtagcaagg
    25021 ttggcaaaga cagtaaagat accgaggacc ttcagatttc tgaaaaatgg agtttcttac
    25081 gcaacaccac catttcaaca ggacttatca tggtgatttt ctacttggtg gcaacagtgg
    25141 cttctgtttt gaggaatgct tcagtaacag aagaattagc agcaggtcaa aacccattta
    25201 tctttgctat taagagtggt cttacctttg cggttggtgt ggcaattgtc tacgccggtg
    25261 ttcgcatgat tttggctgac ttgattccag ccttccaagg tattgctaac aaattaattc
    25321 caaatgctat tccagctgta gactgtgcgg tattcttccc ttatgcgcca accgctgtta
    25381 tcatcggttt tgcgtcaagt ttcgttggtg gcttacttgg gatgttgatt ttaggagttg
    25441 caggtggtgt cctgattatc ccaggtatgg tgcctcattt cttctgtggt gcaacagcag
    25501 ggatttttgg aaattcaaca gggggtcgtc gcggtgcgat gattggtgct ttcgctaatg
    25561 gcttattact cgccttcttg ccagccatgc ttctacctgt acttggtaaa cttggttttt
    25621 caaacacgac ctttggagat gtggatttcg gtgttttagg gattttactg ggccgcttag
    25681 gaacgtcgat tggtcaaatc ggtatttact tggtggttgc tgtgcttact attgtattgg
    25741 tgcttccaag ctttctgtca aaatctcaag aggccatcaa taatgtttca gaagaaatct
    25801 aaaaggagga tatgatgaca aaaaaactaa acattaaagt cttttcagac ggtgcggtct
    25861 tagagaccat gttaaaggat ttacaaacag gtttggtcac aggctttaca accaatccaa
    25921 gtttgatgaa aaaagcaggc atcagttctt atattggctt tgccaaagaa gtgcttgcca
    25981 aaattacaga ctacccagtg tcctttgaag tctttgcgga tgatctcgca agcatggaaa
    26041 aggaagctga gaaaattgcc agtctaggtg acaatgtcta tgtgaaaatt ccagtcacca
    26101 cttcaacagg agaatcaacc tgtcccctca ttcaaaagtt gtcagccaaa ggcattaagt
    26161 taaacgtcac agccattttt acaattgagc agactcaagc tgtggttgat catttaaccg
    26221 ctcgagttcc agctattgtg tctgtttttg ctggtcgtat tgcagatacc ggcgttgacc
    26281 caatgccaat catggaagaa gctctacgca tttgtcgtca aaaagaaggg gtcgaattgt
    26341 tgtgggctag tcctcgtgaa acttataata tctatcaagc tgatcagcta ggcgtggaca
    26401 ttatcacttg taccactgat ttgattgcca agttgcctct tcaaggaaaa gatttagaag
    26461 actattccct tgaaaccgtg caaatgtttc tgaaagacag taccagcctc ggctttaaaa
    26521 ttttagagga tgccaaacat taatattagg agacgaaagc aggcatacca acctgctttt
    26581 tttgctttgt taaagagtat aagtttcgtt taatattttt caaggacatt tcaagagatt
    26641 gcgatctgac agtgaaattt ctgctatttt atggtaaaat gtagcagata cgtcgtttga
    26701 tacaaagagg agcttgtgac aagcgtggca ggttgctttt tgtagcaaaa caacgtataa
    26761 caactgaatc taagcagaga acaggtacct ctttacccct ctgaaaatta aaaggagaat
    26821 atatgtcaaa acaaaccttt acaacaacat ttgcagggaa accccttgtt gttgaagttg
    26881 gtcaagtcgc taagcaagcc aatggggcaa ccgttgttcg ttatggcgac tcaactgtcc
    26941 taaccgcagc tgtcatgtct aagaaaatgg caacgggtga tttcttccct cttcaagtta
    27001 attacgaaga aaaaatgtat gccgctggta aattcccagg aggtttcatg aaacgtgagg
    27061 gacgcccttc aacagatgcg accttgacag ctcgcttgat tgaccgcccg attcgtccca
    27121 tgtttgccga aggtttccgt aacgaagtac aagtgattaa cactgtcctt tcttatgatg
    27181 aaaatgctag cgctccaatg gcagcgatgt ttggctcatc tctggccttg tccatctcag
    27241 atattccatt taatggaccg attgcaggtg ttcaagttgg ctatattgat ggcgagttca
    27301 tcatcaaccc agataaagaa caaatggaag cgtcgctctt agaattaacc gttgctggta
    27361 gcaaagaagc catcaacatg gttgaatctg gtgccaaaga attatcagaa gacatcatgc
    27421 ttgaggctct tttaaaaggt caccaagcca ttcaagaatt aattgccttc caagaacaaa
    27481 tcgtagcagt tgttggtaaa gagaaagctg aggttgaact gttacaggtt gatgcagact
    27541 tacaagctga tattgtggct aagtacaatg ctcaactgca aaaagctgtt caagttgaag
    27601 agaaaaaagc gcgtgaagca gccacagaag ccgttaaaga aatggttaag gctgagtacg
    27661 aagagcgtta cgcagaagac gaaaacctag caaccattat gcgtgacgtg gcagaaatcc
    27721 ttgaacaaat ggagcatgct gaagtgcgcc gcctcattac agaggacaag attcgtcctg
    27781 acggtcgtaa gattgacgaa attcgtccgc ttgatgctgt ggttgacttc ttgccaaaag
    27841 tacatggatc aggtctcttt acacgtggtc aaactcaggc gctatcagtt ttgaccttgg
    27901 caccaatggg agaaactcaa atcattgatg gtctggctcc agagtacaaa aaacgctttt
    27961 tacaccacta caatttcccg caatattcag ttggtgaaac gggtcgttac ggagctgctg
    28021 gtcgtcgtga aattggacac ggtgccttag gtgaacgcgc tcttgaacaa gtcttgccaa
    28081 gcttagaaga atttccttat gccattcgtt tagttgccga agttctagaa tctaacggtt
    28141 cgtcttctca ggcttctatc tgtgctggta cccttgctct gatggctggt ggtgtaccaa
    28201 tcaaggctcc tgtggcaggg attgccatgg gtctcatttc agacgggacg aattatacag
    28261 tcttgaccga tatccaaggt cttgaagacc actttggaga tatggacttt aaagtggcag
    28321 gaactcgtga agggattaca gcccttcaaa tggatattaa gattgcagga attacccctc
    28381 aaatcctaga agaagctctt gcacaagcta aaaaagctcg ctttgaaatt cttgatgtga
    28441 ttgaagcaac tatcgctgaa ccacgtcctg agttagcccc aactgcacca aaaattgaca
    28501 ccatcaagat tgacgtagac aaaatcaaag tggttatcgg taaaggtggc gaaactattg
    28561 ataagattat tgccgaaacg ggtgtcaaaa ttgatattga cgacgaagga aatgtctcta
    28621 tctactcaag tgaccaagct gccattgacc gcactaaaga aattattgca ggtcttgttc
    28681 gtgaagccaa ggtaggcgaa gtttaccatg ccaaggttat ccgtatcgag aaatttggtg
    28741 cctttgtcaa cctctttgac aagacagatg cacttgttca tatttctgaa attgcctgga
    28801 cgagaacagc taacgtgtca gatgtcctcg aagtgggtga agacattgat gttaaggtca
    28861 ttaagattga tgagaagggt cgtgtggatg cctcaatgaa agctttgatt ccacgcccac
    28921 caaaaccaga gaaaaaagaa gaaaaacatg actaaatcaa atgaattaga tattcgctta
    28981 agagctttta tcaatgcgcc tgataatttt ttggatagtg ttgcccttgt caacgctttc
    29041 cataattttc ctgtctgggc tgctaaagaa ccctacgtca tagaggtaga aggtgtcaag
    29101 gttacccctg tttttaccga taaggaagat atgttcctct ttaaagagga acaaaagagt
    29161 gctcaaagtc agtattggct ggagcgttca gctcttgcag tgctagaaga agtcattaca
    29221 tctggtgcag caggtcttgt ctttaacctt aagaaaaagg gagattttgg gaattctacc
    29281 attttcaaaa gcagtgacat gattcaattt atgaaccatt acaccactgt gttaaatacg
    29341 cttatgagtg atgacaatgt ggcggcagat acgatggaaa aggtttacct tgttcccgcc
    29401 tttgtttatc ctaaagatga taaccattac gaccgccttt ttcctaccat gtcaacgcct
    29461 gaaggaaaaa gctatgttcc cgccttttca aatcttcaaa gctttgcgaa atggtacaac
    29521 caagacgatt ttggaggcct tttcaggaaa gctgaaggag tgattctgac ttggacgatt
    29581 gatgatattt atcaaccaag aaatggtgaa aacgagcttg atgaaacgtt cggtgtcgcc
    29641 atcaatcctt ttgatgacca acaaattctt gttgattggt cagaattaga taagtcgtag
    29701 gagttagtat gggttggtgg aaagaaagta tagctattgt taaagctcta gacccagctg
    29761 ctcgtaatag tcttgaggtt atcctaactt atccaggcat caaagcccta gctgctcatc
    29821 gtttgtctca ttttctttgg cgacatcatt ttaaattact agccagaatg catagccaat
    29881 tttggcgatt ttggacacaa attgaaatcc atcctggagc acaaatcgct cctggagtct
    29941 tcattgacca tggtgctggt cttgttattg gagagacagc aattgttgaa aaaggtgtga
    30001 tgctttatca tggggtgacc ctaggtggaa ccggaaaaga ttgtggcaag cgtcatccaa
    30061 cgattcgaca aggtgcctta atttcggcac atgcccaagt gattggacct attgacatcg
    30121 gagcaaatgc taaagtaggg gcagcagctg ttgtgttatc agatgttcct gaagacgtga
    30181 cagttgtagg tgtgccagct aagatagtac gagtgcatgg acaaaaagat aatcgtcaaa
    30241 ttcaaagttt acaaaaacaa cgagaggtct cttatcagtt gtcaaaatga tcggaaaata
    30301 acaaaaagaa gcactgatga cttccaataa aatatttttg gactttctag tagagtaatg
    30361 ttgaacttag ctagtcctat cagtttggtt gctgagtgta gtgatatggg aatgttacaa
    30421 aatacaaaaa acgcgtaaaa gagcatcaac taagcaagtc ttagactgaa gaaacagtct
    30481 attgtgatac tttttctcag gttgtgctta cggcagcgac ttcctttgga attccatacc
    30541 ttagttttga gcctaagatc tcaaaaatcc gtatgggtat cactgtcaat attgatgcca
    30601 cttttaccac aacaaaaagt agctgttttg gctcacccca catgcagaaa taggaaacct
    30661 gatgtcaaat cagtagatct attcatgctt tttgagcata aaagtgggtt tgtctacttt
    30721 ctgaagcctt cacaggttgt gaaggttttt cgttaataaa ggagcctcat gaataaaata
    30781 agagttgggc tgtatgtcat tatgagtttg tctatccttg tttttagtat tacactggtg
    30841 gctagtctaa tttttttact gagacaagac atggttaaag cgattatata cttcattttg
    30901 accctatttt ggttaatagg tttggttatt aatatcaaat tgttgaaaag gtggaacagc
    30961 atttcagaaa aagacttagg atatcaagat gacaactaat gccaggctat tgaataagga
    31021 gtttacatga ttaaaattta tgataccatg acccgttcgc tccgcaagtt tgtacctttg
    31081 actgaaaata cagtcaatat gtacgtttgt ggaccgacgg tctataatta tattcatatt
    31141 ggaaatgcta gatcagcggt cgcttttgac accattcgac gttattttga gtatactggc
    31201 tatcaggtca attacatttc caattttacc gatgtcgatg ataaaattat caaggctgct
    31261 actcaagcag gtgtttctcc taaagaattg tcagatcgct ttattgcagc ttttatagaa
    31321 gataccaagg cacttggtgt taagccagcc acacaaaatc ctcgtgttat ggattatata
    31381 gcagaaatca tttcatttgt tgaaagcctc attgaaaaag attttgctta cgaagcagat
    31441 ggagatgtgt atttccgcgt ggaaaagtca gagcattatg ccaagctagc taataaaacc
    31501 ctgtcagaac ttgaagttgg agccagtggt cgaacagatg ctgagacagc tttaaaagaa
    31561 aatccactgg actttgctct ttggaaatca gctaaggcag gtgaggtttc ttgggatagc
    31621 ccctgggggt ttggtcgtcc aggctggcac attgaatgtt ctgtgatggc tactgaaatt
    31681 cttggtgata ccattgatat tcatggtggc ggagctgatt tggaatttcc tcatcatacc
    31741 aatgaaattg cccaatctga ggccaaaaca ggcaagactt ttgctaacta ttggatgcac
    31801 aatgggtttg tcactgttga taacgaaaaa atgtccaagt cactaggtaa ttttgtgacc
    31861 gttcatgaca tgttacaaac cgttgatggt caggttttgc gattctttct tgcgacacaa
    31921 cagtaccgaa aaccaattaa cttcactgaa aaggctattc atgatgcgga aatcaatctg
    31981 aagtatctta aaaacacctt gcaacaaccg ctgacagaaa ctgcagatga gcaagagcta
    32041 aaacagtttg taatagcttt ccaagacgct atggacgatg atttcaatac agcaaatggg
    32101 attaccgtcg tatttgacat ggccaagtgg attaattctg gctcttatac agaacctgtt
    32161 aaaagcgctt ttgagaaaat gttagctgtt tttggcatta tctttgaaga agaggtgctt
    32221 aaggttgaca ttgaagcctt gattgctaag cgacaagaag cacgcgcgaa tcgtgatttt
    32281 gccacagctg atgccattcg agatcagtta gcagctcaag gcatcaagct gctagatacc
    32341 aaagatggtg tgaggtggct gcgtgactaa tccagttgat gtgaatttga ttaatggtat
    32401 tgccctagcc tttgaagggg atgcggttta ttcctactat gttcgtcgtc atctcatttt
    32461 tcaaggtaaa acgaaaccta gccagctaca ccgtttagca acgagatatg tttctgctaa
    32521 ggcacaagcc aacttgattc aggctatgtt agaagcgcag ctattgaccg aaaaagaaga
    32581 agacatctat aagcgtggtc gcaataccaa tagccatact aaagctaaga atgccgatat
    32641 tattacctat cgtatgtcga caggttttga agccattatg ggttatcttg atatgatggg
    32701 ccaaaaagag cggttagaag aattgatcag atggtgtatt gagtatgtag aaaagcaaca
    32761 attgatatcc tcataagagt cccctaggag actcttattt tcatagatct gaagtaaagg
    32821 tagagagcca ttaagtcttt ttatgacatc aaaaagacat aatgatataa aaaggataca
    32881 tttgtaacaa aaaagacata aaaataatgg aatcattttc gaatagttta taatgagagg
    32941 gtaagactaa tttaaagagg agatatttat tatgacaact atgcaaaaaa cagttagctt
    33001 attatcacta gctttactta ttggtttgct ggggacttct ggcaaagcca tatctgtgta
    33061 tgcacaagat cagcacactg ataatgttat agctgaatca actattagtc aggtcagtgt
    33121 tgaagccagt atgcgtggaa cagaacctta tattgatgct acagtcacca cagatcaacc
    33181 tgtcagacaa ccaactcagg caacgataac acttaaagac gctagtgata atactattaa
    33241 tagttgggta tatactatgg cagcgcaaca gcgtcgtttt acagcttggt ttgatttaac
    33301 tggacaaaag agtggtgact atcatgtaac tgtcaccgtt catactcaag aaaaggcagt
    33361 aactggtcaa tcaggaactg ttcattttga tcaaaacaaa gctagaaaaa caccaactaa
    33421 tatgcaacaa aaggatactt ctaaagcaat gacgaattcg gtcgatgtag gcacaaaagc
    33481 tcaaacaaaa caatcagcta accaagaaat agattctact tcaaatcctt tcaaatcagc
    33541 tactaatcat cgatcaactt ccttaaagcg atctactaaa aatgagaaac ttacaccaac
    33601 tgctagtaat agccaaaaaa acggtagcaa caagacaaaa atgctagtgg acaaagagga
    33661 agtaaaacct acttcaaaaa gaggattccc ttgggtctta tcaggtctag tagtcagttt
    33721 agctgcag
//
LOCUS       DAUCWO010000013        30346 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUCWO010000013 DAUCWO010000000
VERSION     DAUCWO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30346
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.013"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            130..333
                     /locus_tag="VGY20_000562"
     CDS             130..333
                     /locus_tag="VGY20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273440.1"
                     /translation="MSKWELQKQLLENATDFESTVEALLDLNESGEISDLELLLNLKD
                     KFRSYSKEKIRILNEILKLGGAE"
     gene            330..715
                     /locus_tag="VGY20_000563"
                     /pseudo
     CDS             330..715
                     /locus_tag="VGY20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            860..1063
                     /locus_tag="VGY20_000564"
     CDS             860..1063
                     /locus_tag="VGY20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273441.1"
                     /translation="MNYLEYALAYLERELEIIDNDVIEVELPNGGWEFVPNPYYEEGL
                     HDSPYYRSQVAKDILDIKGLLGR"
     gene            1152..1451
                     /locus_tag="VGY20_000565"
     CDS             1152..1451
                     /locus_tag="VGY20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273442.1"
                     /translation="MIEVTFKAESMAGVFDAMREALGMPKTVTKDVVEEEPAPKKKEE
                     SVTLALADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEENQFAEFVEKLEAL"
     gene            1451..2608
                     /locus_tag="VGY20_000566"
     CDS             1451..2608
                     /locus_tag="VGY20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ4273443.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLA
                     MGTLEKLIGKTTFADLLAEVIDKPQGKPVLVKNSDKRPALNSLEQAIKDFE"
     gene            2617..3180
                     /locus_tag="VGY20_000567"
     CDS             2617..3180
                     /locus_tag="VGY20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ4273444.1"
                     /translation="MIMTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKVTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHVFMS
                     VSSKTKPQIIDKYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            3223..5145
                     /locus_tag="VGY20_000568"
     CDS             3223..5145
                     /locus_tag="VGY20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ4273445.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDNSGKNLIRYFSLPCKPSKANGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWGYYACDQRINDRGVALDKELVASALYC
                     KDVKMKSLSGELKAITGLDNPNSRAQLLPWLKEHGYSVNGLTKADVEQELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIVYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYGVVFHVHDEAIIEGSGLTIEEVNDLMAQAPEWAGG
                     LPLNSEGYVTKYYMKD"
     gene            5150..7534
                     /locus_tag="VGY20_000569"
     CDS             5150..7534
                     /locus_tag="VGY20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ4273446.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWRDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDEVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDTTPTNKLPSSK
                     AMNAFVSDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEAACETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYAIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMSEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLARNSFTQRMNELNFENKNAKMEGKTVRCWIGFRLKGEF"
     gene            7902..8177
                     /locus_tag="VGY20_000570"
     CDS             7902..8177
                     /locus_tag="VGY20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ4273447.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGAPDRIVVMNTGIFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMENWV"
     gene            8174..9496
                     /locus_tag="VGY20_000571"
     CDS             8174..9496
                     /locus_tag="VGY20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ4273448.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGARLETSQTRFKDKYFVPDKRNGLIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENVVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            9497..9664
                     /locus_tag="VGY20_000572"
     CDS             9497..9664
                     /locus_tag="VGY20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273449.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREWKK"
     gene            9661..9927
                     /locus_tag="VGY20_000573"
     CDS             9661..9927
                     /locus_tag="VGY20_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273450.1"
                     /translation="MTEELIKPLLEEIERKDDRLYMQSNVIAVLMTAVLMLLMISIVL
                     QNHYEPQITGLRAQLSRTQKQLKRASEDRARQTKRIVELTGNGG"
     gene            10078..10269
                     /locus_tag="VGY20_000574"
     CDS             10078..10269
                     /locus_tag="VGY20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273451.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEICIDEVIGAMKNNDVIALNGKNNA
                     VIIPKQNISHVIIHKEVTG"
     gene            10266..10682
                     /locus_tag="VGY20_000575"
     CDS             10266..10682
                     /locus_tag="VGY20_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEQ4273452.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            10772..11224
                     /locus_tag="VGY20_000576"
     CDS             10772..11224
                     /locus_tag="VGY20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ4273453.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            11214..12491
                     /locus_tag="VGY20_000577"
     CDS             11214..12491
                     /locus_tag="VGY20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ4273454.1"
                     /translation="MTTKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLSSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYVIEEYVRKNLTNDKIANAIKDLGYTKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWVVDERCVKTIEELENYTWKKDKKTNEYTNEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            12507..14040
                     /locus_tag="VGY20_000578"
                     /pseudo
     CDS             12507..14040
                     /locus_tag="VGY20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            14000..15448
                     /locus_tag="VGY20_000579"
     CDS             14000..15448
                     /locus_tag="VGY20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ4273455.1"
                     /translation="MLVVKKNQRQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKVASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKRLKQGFVRGEHPRAMARDLAKR
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRTIAKENK
                     RKLLSELKPGINVAPYHFNCRTTIIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHSVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HIKTPDYLVDVAPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMIDRRGLDISIIKNKDEVLAVLEKEGG"
     gene            15476..15664
                     /locus_tag="VGY20_000580"
     CDS             15476..15664
                     /locus_tag="VGY20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273456.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKEG"
     gene            15669..15935
                     /locus_tag="VGY20_000581"
     CDS             15669..15935
                     /locus_tag="VGY20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273457.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEAIKNQNKQIMELKSIVQ
                     RNALATNEELAAVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            16103..16672
                     /locus_tag="VGY20_000582"
     CDS             16103..16672
                     /locus_tag="VGY20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ4273458.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEYPKHEYERTFTRADISKMMAAE
                     RTKWESEQSAAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLATTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            16685..17572
                     /locus_tag="VGY20_000583"
     CDS             16685..17572
                     /locus_tag="VGY20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ4273459.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            17584..17940
                     /locus_tag="VGY20_000584"
     CDS             17584..17940
                     /locus_tag="VGY20_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ4273460.1"
                     /translation="MGDKQLIDDIKLFIGISKGDGAQDELITLAIYESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            17951..18229
                     /locus_tag="VGY20_000585"
     CDS             17951..18229
                     /locus_tag="VGY20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273461.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKSRLIKGLAHHKNSTVIYV"
     gene            18226..18570
                     /locus_tag="VGY20_000586"
     CDS             18226..18570
                     /locus_tag="VGY20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ4273462.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQSFLDPALRKEWP
                     VLMANIKKMFKR"
     gene            18574..18933
                     /locus_tag="VGY20_000587"
     CDS             18574..18933
                     /locus_tag="VGY20_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273463.1"
                     /translation="MDYSLETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            18945..19544
                     /locus_tag="VGY20_000588"
     CDS             18945..19544
                     /locus_tag="VGY20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ4273464.1"
                     /translation="MANTKKGTTIEITTGKPIVGKKIFYFIQSVDAPKKSQALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQIPESTGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            19598..20053
                     /locus_tag="VGY20_000589"
     CDS             19598..20053
                     /locus_tag="VGY20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273465.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAESVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDMDATQIQIIEDMIGRMSNAVS"
     gene            20128..20361
                     /locus_tag="VGY20_000590"
     CDS             20128..20361
                     /locus_tag="VGY20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273466.1"
                     /translation="MEGLEYKQLAERESLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            20376..24758
                     /locus_tag="VGY20_000591"
     CDS             20376..24758
                     /locus_tag="VGY20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ4273467.1"
                     /translation="MPGTFDGSIFADVGANTKDYEQAMARIVSTTQNAFRKAQDTAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVTDKIRGNLTGFINQTKMQLQNLVGIGKIRSAFAKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGQQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVAQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFSQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLRDFAHTGELSGQAFDNLKAKITSLAPKVIALWA
                     VMNPASAISTIMPLLSLFGKVALALGSLGTSVGAFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVLGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IEQLLNTAVTKGPGIIQGLVKGITSKIPVLIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATNLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIDSFGNSLQANLPSIISNGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIAQSAVQIIQFLASGLTQALPQIIAAG
                     IQLVAQLAVALIKGLPQIISAGIQLIMGLGKAMLGAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATVMAMNVGMQTTAIANQANTDTTSMLNSISQNTELANINATS
                     NATQMANNVNAQTGTMSLRGLNDSMSLASGISANMSVANINATTQAREMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGAASTGATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSVTSEMSSAATSATSSVNRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFD"
     gene            24770..25612
                     /locus_tag="VGY20_000592"
     CDS             24770..25612
                     /locus_tag="VGY20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4273468.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTISNVERLLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFALRTAELP
                     FGETKPKDITLSGGSFAYAGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            25622..27601
                     /locus_tag="VGY20_000593"
     CDS             25622..27601
                     /locus_tag="VGY20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4273469.1"
                     /translation="MITFLDHRDIEYGVISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLTRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINQRTDKELEEFRAAL
                     KNLALPEEAIKKITEAIKVDDIPSIKQSFDDLKNRVSETSEESRLTAEILGNNGKTRY
                     NKNLLVGDPNRVKTYDQDYIEVEANDGGFKRGETYTISFSQTCELLKEVAITLTQINN
                     KGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLSYTGVLTGDWYKPKQIDLNASDTQE
                     LALEMDYKDVVDGKDATITGAWSDSPQIILYGGKK"
     gene            27598..28713
                     /locus_tag="VGY20_000594"
     CDS             27598..28713
                     /locus_tag="VGY20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ4273470.1"
                     /translation="MTETIPLRVQFKRMKAAEWASSDVVLLEGEIGFETDTGFAKFGD
                     GQNTFSKLKYLTGPKGPKGDTGLQGKTGGTGPRGPAGKPGTTDYDQLQNKPDLGAFAQ
                     KEETNSKITKLESSKADKNAVYLKAESKIELDKKLNLKGGVMTGQLQFKPNKSGIKPS
                     SSVGGAINIDMSKSEGAGVVVYSNNDTSDGPLMSLRTGKETFNQSALFVDYSGKTNAV
                     NIAMRQPTTPNFSSALNITSGNENGSAMQIRGVEKALGTLKITHENPSVEANYDENAA
                     ALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIRNLGDDKFYVKSDGGFYAKKTS
                     QIDGNLKLKDPTANDHAATKAYVDKAISELKKLILKK"
     gene            28728..>30346
                     /locus_tag="VGY20_000595"
     CDS             28728..>30346
                     /locus_tag="VGY20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ4273471.1"
                     /translation="MSRDPTLILDESNLVIGKDGRVHYTFTAEDDNPKVRLASKCLGT
                     AHFNQLMIERGDQATSYVAPVVVEGTGNPTGLFKDLKEISLELTDIENSKLWSKIKVN
                     NRGMLQEYYDGTIKTEIINSAQGVATRISEDTGKKLALINDTINGIRREYQNADKKLS
                     ASYQAGIDGLKTTMANDKIGLQAEIKASAQGLSQKYDDELRKLSAKITTTSSGTTEAY
                     ENKLNGLRAEFTRSNQGMRTELESKISGLRTAQQTTANQISQEIRNREGAVSRVQQDL
                     ASYQRRLQDAEDNYSSLTHTIRGLQSDVGSPTGKIQSRLTQLAGQIEQRVTRDGVMSI
                     ISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNTTVNGTFT
                     TKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLNTAKMDFNSDATINFNSKNNALVRKDGTHTAFVHFSNATPKGYRGS
                     ALYASIGITSSGDGIDSASSGRFCGVRFFRYA"
BASE COUNT        10059 a         5348 c         6897 g         8042 t
ORIGIN      
        1 ctgttgcttt agcaattgtt gccaggatac aatatgaagt catcaaaaaa cacaactcgc
       61 cagagaataa gcgaaaaatt tttagggaag tagctttaga aaacagcaag aaatggagtg
      121 cagaacgtta tgtctaaatg ggaattacaa aaacaactac tggaaaatgc gacagatttt
      181 gagagcacag ttgaggcttt acttgactta aacgagtctg gcgaaatatc tgatctggaa
      241 ctcttactga acttaaagga taaattcaga agctattcca aagaaaaaat acgtatcctt
      301 aatgaaattc tcaagctagg aggtgcagaa tgatgacaac ttttgaaaag attattaaga
      361 aaattaatcg gattgagggg gagttagtta aacctgaagc tgaaagacga gatagcttac
      421 tatcctatct gctcagttgg gttctattaa acaagattta aaattgatgc tttggattga
      481 gctaccgacg ttaaatgacg tagagagata tgaagctctt tatcaaggaa atgattttaa
      541 attcagtgaa actttcttag agcgtcaagc gacgagaaaa gccttttgga gacgattagc
      601 taaagaaact ttcaaagaag accaggataa gaggaacttt gttaagttgg ctgagaaaga
      661 atttaaaggt gcaactttgt catggcaaga atttttgtgg ggtaaaaaga aatgaatata
      721 tcactaaaat ttattgatga aaccctagct ggcttaaatg atattttacg tcaggggggg
      781 gctcttgctc ccaaaaccaa gccctggcgg acgcagtttt tatactaaca gttttaaaac
      841 aggtaattga ggagagaaaa tgaactattt agaatatgca ctagcttatc ttgaacgtga
      901 gttagaaatt attgacaacg atgttatcga agttgagttg cctaacggag gttgggagtt
      961 tgtgcctaat ccctattacg aggaaggttt acacgatagc ccatactatc gcagccaggt
     1021 tgctaaagac attcttgata ttaaaggact tttggggagg tgaggctatt gattgcatca
     1081 aaaaaagacg gcttaggaaa ccgtcaacaa ttatacacaa ttaaattata acacagaaag
     1141 agagaaacac aatgattgaa gtaactttta aagcagagag catggcaggt gttttcgatg
     1201 ctatgcgtga agctctgggt atgccgaaga cagtaaccaa ggatgttgtt gaagaagaac
     1261 cagctcctaa aaagaaagaa gagtctgtaa ctttggcatt agctgatatt aagaaaatgg
     1321 ctaaagccaa ggttgaagaa gggaagtcaa aggacatcaa ggaagtcttg aaagagatga
     1381 acgtcgcaaa agttggtgaa cttgaagaaa accagtttgc tgagtttgtt gagaagttgg
     1441 aggccctcta atgccagtag aaaatcacgc actactgtct gcttctagtg ctcacagatg
     1501 gctttattgc cctatgctgc cgagattaga agcagactat cctagccgcg ataccgtata
     1561 cactcaagag ggcacaagcg cccatgagct ttctgaaatc aaactgatgt acaagtctgg
     1621 taaaatcacc aagcgtaaat ttaacacgtt aaccaaggct tttaaggaaa actcagactt
     1681 ctacaacgaa gagatggaag agatgacgga actttacaca gatattgtta tggagcacct
     1741 aaatgcttat gagaacgccg aaattgaact tgaaaaacgg gttgatttta gcgactgggt
     1801 gcctggcggt tttgggactt cggatgttgt cattttagcg gatggggtca ttgaaattat
     1861 tgatcttaag tatggcaagg gcatgcctgt gtctgctaac caaaacccac agatggggct
     1921 gtacgctcta ggagcttatg cttcctacga tatggtttat gactttgacc gtatcaaaat
     1981 gaccattatt cagccacgtt tagattcggt tagttctgtt gacatttacg tggaggagtt
     2041 actctactgg gcggataatg ttgtcttgcc tatggccgct caagcagacg caggcattgg
     2101 tgactggaac ctaagtgaaa aagtattgca gtggtctcct gtcgcagcta aattggtacc
     2161 aagagcgcaa gaaaattggg aattaattga taaatatgac tatcaggagc ctgtctattt
     2221 atctgatgaa gccgtcgcag agattcttga caaagcctca gctatcaaga agtgggttga
     2281 gtcagttgaa gcctatgccc tgaaagaagc actctcggga aaagaagttc caggctataa
     2341 gattgttgaa ggtagaagta atcgagtcat cactgataaa gacaaagcaa ttgatatttt
     2401 acaagataac ggctttgacg atgagatttt taaaccgaaa gagctgttag caatgggaac
     2461 tcttgaaaag ttaataggta aaaccacttt tgctgattta ttagcagaag taattgataa
     2521 accacagggc aaacctgtcc ttgttaaaaa tagcgacaag cgacccgcat taaatagctt
     2581 agaacaagca attaaagatt ttgaatagga gaaattataa ttatgacacc aaacacaact
     2641 aaagtagtaa ccggaaaagt acgcctaagc tatgtagctt tactagagcc taaagccttt
     2701 gaaggtcaag aggctaagta ttcaacagtt atcttaattc caaaaacaga caaagtcaca
     2761 atcaagaaaa ttaaagacgc gcagaaagct gcttatgagg ctgccaagga caataaactc
     2821 aaaggggtta aatgggagcg cgttaagaca acgcttcgtg acggcgacga agaaatggat
     2881 accgaagagc acccagagta cgctggacac gtgttcatgt cagtgtcaag taaaactaag
     2941 ccacaaatca ttgacaagta taaaaacccc gttgactcag cggatgaagt ttactctggc
     3001 gtctatgctc gcgtatcact taatgcctat gcttacaaca cagcgggaaa taaaggaatc
     3061 tcttgcggat taaacaacgt ccaaattgtt gctaaaggag actaccttgg cggccgttcg
     3121 tcagctgatg cagattttga cgagtggaac gaagaagagg acgaagacga cattttatag
     3181 tagagggcct ctttagaggc tcctcatttt taaaggagag gtatgagaca tttaaatatt
     3241 gatattgaaa cctatagttc gaatgacatc aaaaatgggg tttacaagta cgctgacgca
     3301 gaagattttg agattttact tttcgcttac tctatagatg gcggagaagt agagtgtctt
     3361 gatttgacaa ggcagtctct acctgaagac atcaaagata tgttatttga tgataaagtc
     3421 cgaaagcacg cctttaatgc ccaattcgaa agagtttgtc tcagtcgtta cctcggtcta
     3481 ccttactatc tagatccttg ccaatggcaa tgcactatgg tgctggccca agagttgggt
     3541 ctgccttcaa gcttggaaaa gtgtgcgctg tatttaaaat tagcgcagga aaaagataac
     3601 tcgggtaaaa acttaatcag atacttttcc ctgccttgca aaccaagtaa agctaacggt
     3661 gggagaacta gaaatttacc agaacacgcc cccgaaaagt ggcaaatgtt tattgactac
     3721 tgcatccaag acgttgttgt tgagatggca attgccgaaa aactagagtc agttcctgtg
     3781 cacgaccgtg aatggggcta ctacgcatgc gaccagagaa tcaacgacag aggtgtggcg
     3841 cttgataaag agttagttgc ttcggccttg tattgtaaag atgttaagat gaaaagtttg
     3901 tctggtgagc taaaggccat aacagggctt gataatccta atagcagggc gcagttgcta
     3961 ccgtggctaa aagaacatgg ctattcggtt aatggtctga ctaaagcgga tgttgagcag
     4021 gagcttaaga cggccgaagg agaacttaag agagttttag aacttaaact acaaaccgct
     4081 atgtcaagtc taaaaaaata tgaagctatg gaaagagcta tgtgctcaga cggacgagtt
     4141 catggactac ttcagtttta cggagctagc aggacaggaa gatgggcggg cagagttgtc
     4201 caagtacaga acttagctag gaattatata aaggacctag atgatgccag agagtacgtt
     4261 aaaaagcgtg atattgatgc tgtggagatt ttatacgata gtcttaacga cactttaaag
     4321 cagctcgtac gaacggcact cgtggccaaa gactgctgta ccttctatgt ctctgacttc
     4381 tcagcgattg aggctagggt gattgcgtgg tttgctggag agcagtggag gcttgacgtg
     4441 ttttcgacgc acggaaaaat ctatgaggca tccgctagcc agatgttcgg aattccaatt
     4501 gaggagattg ataaggaact acgccaaaaa ggcaaaatct cagagttggc acttggctat
     4561 caaggaggtc ctggagcgct caagcagatg ggagctctaa atatgggagt caaggaagag
     4621 gagctccaag ggctagttga tgactggcgc agggccaata agaaaatcgt ccaattttgg
     4681 aaagatgtgc agagagccgc catcaaagct atcaaatcga gagcaccaat aaaacttgga
     4741 aaactacgat ttagataccg taaaggtttc ctctttataa cattgcctag cggtaggaac
     4801 ttagcttatg caagagccaa ggttgagcca ggcgactatg gagacaaaat cgtctatgag
     4861 ggccaaggag ataaggccta cttcacagcg caagagactt acggcggtaa gcttgtcgaa
     4921 aatatcgttc aggcgacggc cagggatatt ctagctgaag cgcttctgag gattgaagct
     4981 gcaggctatg gtgttgtttt ccacgttcat gatgaggcta ttatcgaagg ctcaggcctg
     5041 acaatcgaag aagttaatga tttgatggct caggctcctg aatgggcggg gggtcttcct
     5101 ttaaatagcg aaggctacgt aacaaagtat tatatgaagg attagataga tgaagcaaga
     5161 aaaactaata gtaaagtctt ctcctttgca agagcttcat atcgcaacgg gtaattcacg
     5221 aacagctaag acgtggaaaa atattacgct aacttggcag gagctggttg agaggttaga
     5281 gaaacctaca gtcacccaag agacgtttgc ggagtaccag aagatgtctc gagcagaaaa
     5341 agggcaagca aaagacgtag gaggttttgt cggcgggtgg ctaaagcagg gtaaacggaa
     5401 aaacgaaaat gttcaaagta ggtccttggt tgcgcttgac gcagatagcc caagtaaaga
     5461 tttcttagat aggctagacc tgcttgcaga ctacgcatac gtactctaca gtactcacag
     5521 ccactcaaaa aaagctgcta agtaccgtct tattatccct actgaccgtt taatgatgcc
     5581 tgatgaatat gagccagtcg ctaggtactt ggctaatcaa ctaggcatgt cgaactttga
     5641 tgacacgact tatcaaagtg tgcgtttgat gttctggccg agccactcga gagatgctga
     5701 ctttacgttt aaatataacg atgaggcttt tctaagtgtt gatgaggtgc ttgatacata
     5761 cccggactgg cgtgactcaa gcttctggcc agaaagcccg acgcacgctg ttaaaagaca
     5821 gcgtgaagct aaaaaacaag gtgacccact tagtaaaaaa gggcttattg gagccttttg
     5881 tcgtaactat gacattagac aggccattgc aacgttcttg cccgaggttt atgaagaagg
     5941 aacgactcct gataggtaca cctacactga gggttcaacc gcaaatggct tagtcatcta
     6001 tgatgaagtc ttcgcttata gccatcacgg gacagatccc gtgggggata cgcttgtaaa
     6061 tgcatacgac cttgttcgta tccataaatt cggagagcaa gatagcgagg ctaaagatac
     6121 tactcctact aataagttgc catcaagtaa agcgatgaat gcttttgtct ctgacttacc
     6181 cgaaattaaa gactatttaa tggcggaggc tttaggcgat ttcgatgaag agttaccagt
     6241 cgaagatgac agaagctggc ttgaaattga tgagaggggc gaacctgagg tcaatagtta
     6301 tttgctagca acgcagatta ttaaggaggt tccgatttat tgggacggct tagaattttt
     6361 acgctacgac gctaaaaaag gcatctggtt gccaaacgca gaggagtatt tgaagagtta
     6421 tatctcaact aagaaactcg gtaaaattac taagattagg cacattagcg aaaccgtcgt
     6481 agcgattaag gcacaggctt tctcaagcga agtgtttact gagagcgatc ttaacaagat
     6541 agtgctagcg aacggagtct atgacttgag ggataacagt tttaagacta agtttgatcc
     6601 agaattgcat gcaaggtcaa gccatcccgt tgtctatgac cccgaggcgg cctgtgaaac
     6661 ctttgagggt tttcttaggg agaccgtcgg agctgaaaat atagatttca tctttgagtg
     6721 gttcggctat aacttttatc gcgaatatgc tattcaaaaa atgctattca tctacggcag
     6781 cggcggtact ggtaaatcaa cactgattaa tattttacgt gaaatgatag gtgctgataa
     6841 ttactcagcc gtgacactgc agtacctgat gcaagaacgc tttgcaaaaa tcggcttata
     6901 tcgaaagact gctaacttcg ataccgatgc taaacctcaa tacttagcag atggcgcaac
     6961 acttaaaatg ttgacggggg aggatactat tcacgcagac cgtaaaaata aagagccgat
     7021 taacttttac aattatgcta agctgtcttt tgccatgaat gagctcccac ctatgcgaga
     7081 tttcagtggg ggacttaagc gccgcatgat gatcctcgag atggataagg ttttaacgca
     7141 ggaagttaag gcgaaatacc cgctagataa gattatgagc gaggtgccag gtatctttaa
     7201 tagggcgatg gaggggctta gaaaggcctt aagtaagaga gatttcagta ttagtgccag
     7261 catgaggtca agtgtcgaga aatgggaaaa aggtaacgat gtcgtggcca tgttccttga
     7321 agacgagtgt gaacttggtg aagacttcaa agttcctgtt agggatgtct acccagccta
     7381 caagttctat tgtcaggatt caggctacaa acctttggct aggaattcgt ttacgcaaag
     7441 gatgaatgaa ctgaattttg aaaataagaa cgcaaaaatg gaaggcaaga ccgtaagatg
     7501 ttggattggt tttaggctaa aaggagaatt ttaaagggtt acgtttttca acaaaaaagg
     7561 taaacaggag atataaggtt acgtagttac gcagaattca gactgataaa aaattttata
     7621 agttgaaaaa aaacgtaact acataacttt cctgaaaaac ctttcctgtt taagtcaatt
     7681 tacataacta acgcaacttt tatgcaacct caaaaaacct ttcatagcaa tagtttagac
     7741 cctaaggtta tgtagttatg ttttttcttc tattaactta aatataaata taaaaaataa
     7801 atatatataa aataaggtat atataaaaca cgtatataaa aaagaattaa taagttgaaa
     7861 aaaaaacgta accctgacct ttattgacca aagggggaag gatgaggact gaaaaagata
     7921 ttgaaaatta tttgaaaaag aaaacaaaag ggctgtgttt aaaattcacc agtccaggga
     7981 cgataggagc gcctgacaga attgttgtca tgaatacggg aatctttttt gtagaggtca
     8041 aagcgcctgg taaaaaacca agacccagcc aagttgccat gcacaaaaaa ataaaagagg
     8101 ctgggcagca cgtttgggtt gttgactcct acgaatcagt ggacatggcc ttaaaagaaa
     8161 tggagaactg ggtgtgagac tgcacgagta ccaagaatac gctaagacat ggatagtaga
     8221 gcacccttat tgtggccttt tacttgacat gggccttggt aaaacgctga caacactatc
     8281 ggcgatagat gagattcaaa atattttttc cgaggatcat aagattttaa tcgtagctcc
     8341 taaaaaagtg gcggaagaaa cgtggccaac ggagattggg aaatggcatt ttgatttcac
     8401 ctactctaaa gttttgggga gtgagggaaa acgaattgaa gccttagaaa cagaagccga
     8461 tatctatttg attaatcgtg agaatgttac ttggcttgtt gaatactaca agactaaatg
     8521 gccgtttacc tttgtggtta ttgatgagct gtcaagcttt aagtctagta agtcaaaacg
     8581 gtttagggct ttgcgaaaag ttagaccgaa agtccaacgt cttgtaggac taacaggaac
     8641 cccagcgcct aacagtttga ttgatttgtg gccgcagatt tatctgatgg acagaggcgc
     8701 cagacttgag acgagccaga ctcgatttaa agacaagtat tttgttcctg ataagcgtaa
     8761 tggcctaatc atttacagtt gggcacttag ggatggtgca gaagcagaaa tctataacaa
     8821 gattgaggac atctgtgtca gcatgaaagc taaagactat ctcaagttac cgccgcgaac
     8881 caacaacgtt gtatcagtta agttatctaa tatgaaagcc tacaaacagc ttgaagctga
     8941 tttggtgttg gagtttaaaa ataaagaaat atctgcggct aattctgcgg ttttggccaa
     9001 taaattactt caaatggcca atggtgctat ctatgatgat gataaagcaa cagttgctat
     9061 acacgacgac aaacttgacg cgcttgagaa tgtcgttgaa gaaagccaag gtcagccaat
     9121 cttagttttt taccagtatc aacatgatct tgaaagactt aagaaacgat ttcctcaggc
     9181 tgaagagctg acgtcggttg acaagtggaa ttccggaaaa ataccaattc ttctgtgtca
     9241 cccacagtca gccggacatg ggcttaatct tcaaaaaggc gggcatatta ttgtttggtt
     9301 tgggctaaca tggagtcttg aatattatca acaagctaat gccagattag atagacaagg
     9361 gcagacagaa cccgttattg tacaccacat tgttgcagaa aatacagttg atgaaaaagt
     9421 acttaggatt ttacaaggca aagaaaaaaa tcagaacgcc ttactcgaag cagttaaggc
     9481 gcagttagga gtctagatga aaaaagaata tgtcgttaga atctacacgg gtagagaaaa
     9541 gaattttgag gcaaaacctc agtttgaaga gaaaaccttt acgcgaaaag cggacatgtt
     9601 gaagttttgg aattcttgcg aagcaacagt taaagaaaaa tatactaggg agtggaaaaa
     9661 atgacagaag aactaataaa accgctgcta gaagagatag agcgaaaaga tgatagattg
     9721 tatatgcagt ctaatgtaat tgccgtctta atgacagcgg ttcttatgtt actgatgata
     9781 agtatagtct tacaaaacca ctacgaacca caaataacag gactgcgtgc tcagctaagt
     9841 aggacacaaa agcagcttaa acgtgctagt gaggataggg ccagacagac aaaacggata
     9901 gtggaattga cgggtaatgg ggggtaggta tgaatataat ccatagaatg gaaaacggga
     9961 tagatgctgt aggtgattta acaacttatg cagtaccatg ttgtccagag tgtaaaaatg
    10021 aaacatatag tcagaaacac tgtccttttt gcggtgaaaa tcttatggag gtagcaaatg
    10081 aaaattgaat tttttttaaa gcaaggtcct actaagagcg tttctaatta tggtgaaatt
    10141 tgtattgatg aagtaattgg cgcaatgaaa aataacgatg tcattgctct aaatggaaaa
    10201 aacaacgcag ttattatacc caaacaaaat atttctcacg taattattca taaagaggta
    10261 acgggatgag tagattatct aatgcacagc taaaggcttt tgatgaatgg ttgtttgatt
    10321 atcgttttat tgataacaag attgcacttc gaaaacttga gctacaaact gatatgaata
    10381 cagaagtaga taaaaatatc ggtggaggaa gagcaaattt tgtttcaaaa gttactgagg
    10441 atgtggttgc aaggtgggat tcagataggg aactaaaggg tctatccaat ttcaaagaag
    10501 cagttattgc aacgctaaat cttttggatg aagagctaac tgatattttt aatcttaggt
    10561 ggggtgttgg ttcaagtaac acttgggaag aaattgccta tacaagacat ctgtcacgta
    10621 agtctattta ccgcaaaaga gagcggattc tagaaatatt ctccaaaaaa ataggaatgt
    10681 gacacaaaaa tgtatggaat tgtcatcttt ttgatgataa attgatagta tgagtttaaa
    10741 ggtaaggcaa atagtgtctt gcctttttgt tatgcttgga ggtgatggaa aattgagtaa
    10801 attaacccta aaacagaagc gttttgcaga tgagtacatc atctcagcta acgcgacagc
    10861 agctgctatt aaagcagggt atagtaaaaa gacagcaagg tcaataggtc aagaaaactt
    10921 gaccaaacct gacattaaag cttatataga cgagcggctt gaaaaacttg aatctgaaaa
    10981 gattgctacg caagaagaag tcctacaata tctcacttca attatgcgag gagaccaaca
    11041 agaaaagacg ctcattagcg ttggagagtt tggccagaag atagttgaca tcgatgttgg
    11101 tgccaaggat agaatcaagg cagctgagct actaggcaaa cggtacaggt tatttactga
    11161 caaggttgaa atggatgtca gctcagatgt aaccattaac gtaggtgagt gggatgacga
    11221 ctaaacagcg ccctaaaatt aatatcgtaa tcaagcatcc tagcaaagtt tttaacaagc
    11281 atatctacga caaactttac aactatagca acttcacgga agttcattat ggtggggcgt
    11341 ctagcggaaa gtctcacggt gtttttcaaa agataatttt aaaagcactt aaccccaaat
    11401 tcaaacatcc cagaaagata ttagttctca gaaaagtcgg tgcaaccgtt agagactctg
    11461 tatttgctga tatcatgtct aatttgtcgt atttcggcat cttggataaa tgtaagataa
    11521 atatgtcggc ttttagaata acgctcccga atggctcgga attcattttt aagggtatgg
    11581 ataaccctga gaagattaag tcaattaagg ggatatctga tgtcgtcatg gaagaagcta
    11641 gtgagtttac gcttgatgat tatacgcagt tgactttgcg tttaagagat aagaaacatc
    11701 tagagaagca aatatacttg atgtttaacc ctgtgtcaaa agtaaactgg gtttataaag
    11761 ccttcttcgt taagacacct aagaatacag ttgtctatca aacaacttat aaagacaacc
    11821 gcttcttaga tgatgtcacg agagaaaata ttgaggaact agccgatagg aatgaagctt
    11881 actacaaaat atatgctctt ggccagtttg ctacacttga taaactaatt tttcccaaat
    11941 atgacaagca aatattaaac aaagacaagt tatcacactt gtcttctttt tttggtttgg
    12001 actatgggtt tatcaatgac ccttcggcat ttttgcatgt taaaatcgat gacgcaaaca
    12061 agaagttata cgtcatagag gaatatgtca gaaagaattt gaccaatgac aaaatagcaa
    12121 atgctataaa ggacctcggc tataccaaag aagaaatcag aggagattcg gctgaaaaga
    12181 aatctaatca agagctgagg aatttaggta ttcctagaat gattgatgtt accaaagggc
    12241 ctggaaccgt tatgcaagga atacagtatt tgcttcaata tgattgggtt gttgatgaaa
    12301 ggtgtgtcaa gactattgaa gaactcgaaa attacacttg gaagaaagac aagaagacta
    12361 atgagtatac caacgaacca gttgacagct ataaccactg cattgatgcc ataagatatg
    12421 ccgtacaaga cagaatatac cagtcggcgg atagaagtaa gcgcatgaaa aatgctaaat
    12481 attattttta ggaatgaggt gaaactttgg aagaaaaaca atttttagca ggaactcgtt
    12541 ttaatgagaa tgctaataag caatttatta tgctgcaaga agactttgaa gcaattgact
    12601 atgaatcaaa actttggata gatcaactta agaattacat ttcaaggttt aaagcagaac
    12661 aattagagcg tttgaaagag ttaaagcgat actatcttgg ggataataac atcaagtata
    12721 gacctgctaa aacagataaa tacgcagcgg ataatcgtat tgcaagtgat tttgctaagt
    12781 atattacggt atttgagcaa ggttatatgc ttggcgttcc cgttgagtac aaaaatgaaa
    12841 ataaagacct ccaggcagct attgacctta tgtcggtcag aaacaatgag gactaccaca
    12901 acgtcaaaat aaaaacagat ttatcaattt atggaagagt ctacgagctg ttaactgttg
    12961 agaaaataga tgacaaaaaa aaccgaagta aaactctacc agctcccggc cgagcaaact
    13021 tttgttatct atgatgatac ataccagcga aattcgttga tggccgttca tttctacgac
    13081 atagattacg gctcaggtaa acgtaaacag attatcaaag catatacttc cgacacaatt
    13141 tatacctacg aagactataa ccttgaaaca aaaggtatgc gattaaaaga ttatgaagga
    13201 cattttttta aaggtgtacc agtcaacgaa tacgctaata acgaggagcg aacgggggct
    13261 tatgagtctg tacttgataa tattgacgcc tatgatttat ctcaatcaga gcttgctaac
    13321 tttcaacaag attcagtcaa tgcgttgctt gtaatagctg gtaatgctta cacaggagct
    13381 gatgagaatg actacttaga tgatggccga ttaaatccta atggtcgttt agcgatctca
    13441 attgggttta aaaaagccca agtgttaatt ttagacgata atcctaatcc aaatggcgtt
    13501 aaaccgcaag cgtactttct caaaaaagag tatgataccg ccggtagcga agcctacaaa
    13561 aatagattag ttgcagacat tttgaggttt acttttacgc cagatacaca agatatgaaa
    13621 ttttcaggag ttcaatctgg tgaatcaatg aagtataagc ttatggcttc tgacaactat
    13681 cgcgagaagc aagagaggtt gtttaaaaaa ggattaatgc gacgcttacg cttagcagct
    13741 aatatctggg ccatcaaagg gaatgaagca actacatata gccttgtaaa tgatacgagt
    13801 atagttttca cacctaatct tcctcaaaac gataatgaaa ttgttacagc tgcacaaaat
    13861 ctttatggca ttgttagcga tcagactatc tttgaaatct taaatactgt cacaggagta
    13921 gatgctgaag cagagcttaa acgcttgaaa gaagaagctg ataaaaagca atctttaccg
    13981 gagcctagat tggtaggtga tgctagtggt caagaagaac caacggcaga aaagccttaa
    14041 ttactggcaa aagcgtcaag aagatattct aagttattta gacagaactg atttagatgt
    14101 cttttcagaa ctgcagaagt tatataatga acaggctttt gagttacaaa aggaattgtt
    14161 tgatttttat actaagtatt ctgaagagaa caaaatgact tatcaggatg tcgttaaaaa
    14221 actacgtcat gaagatttat cagattacgt agctaatgct aacaagtatc gtaaacaagc
    14281 tgagaaagat cctgagctgc taaaacgact taatgagcag tatgtatcag caagagctac
    14341 aaggatggat gcattaaatc ttgagcttgt ttatcgtgcg gggatactaa aaggtgtact
    14401 tgattcagca tttgaaaatc atttaaaaaa agttgcctca tatgcttata aaaaagcaat
    14461 gggtggacgg tcagggacaa tcaatggtcc agttttagaa gaactggtta gaaccccgtt
    14521 tgatggttat aactattcag aacagttgtg gggcaatact gacaatcttg ttaagaatct
    14581 tcaaaagaga ttaaaacaag gttttgttcg tggcgagcat cctagagcga tggccagaga
    14641 tttagctaag cgatttaatg ttgctaatca cagggctgaa acactaatca gaactgatgg
    14701 aactatggtg attaataatg ctaccgctag gcgctacttg aatgcaggac ttaagtatta
    14761 tcgtgattta gttagacttg atgacaggac aactgagata tgccgtacaa ttgctaaaga
    14821 aaacaaaaga aagctattat ctgagctaaa gcctggaatt aatgtagcac cctatcattt
    14881 caactgcaga acaactatta ttcctgatga agatgagcta agtattgaag tagaaccaat
    14941 tgatgataaa agcactaagt attttaagga tgtcacctca gattggatag atggaaatga
    15001 gcataaacca caactgtcac tactaaatga gtacgtaaaa aatggcactc cttacaaagt
    15061 agatggccat agtgtcgttc ttgatcattc gaattatgaa tatagagttg ctaattggtt
    15121 atccaaaaag acaggattgc aagttgacat ggtgccaaga gttaattccc ctgaacatat
    15181 caagactcct gattatttag ttgatgtagc tccttttgat ctaaaagaaa tcacaggttc
    15241 gggtaaaaac gtcattgatg gcaatctacg aaaagcaaaa aagcaggcaa ctaatatcat
    15301 ttttgatata acaaaaaccc ctttatcatt tgaagagata atggggcaat tagaacatat
    15361 ttatatgata gatcgtaggg gtcttgatat atcaattatc aaaaataaag atgaggtatt
    15421 agctgtatta gaaaaagaag gaggatgacc caccgcctcc acagtaaact gcttcatggg
    15481 cgttagacca tcatccttct ttatctcaat tatacatcac cctattaaaa ctatcaagga
    15541 ggaaaatatg tttatttggc aaatggtatt agtggcacta ggtgtattgg tactcatatt
    15601 gattgttggc attgcagcaa tagcggttaa gtcaattatt gcagaattga aaaaagaagg
    15661 ataaaacaat gaataagcgc attaagaaaa aacgtaaatt ggaaacagcg gttgtgatgc
    15721 ttgttgcaga gaatgccatg caagcagaag ctattaaaaa tcaaaacaaa caaatcatgg
    15781 agctaaaatc aatcgttcaa cgaaacgctc tggcaacaaa cgaagagtta gcggctgtta
    15841 aagctgctac tttagataac caatcagtta tcaaggcaat tggtgacacg gttgactata
    15901 ttaagaaaaa ctacaaacgg aagtggggga aataaagttt aaccgtgtcg aatttgacct
    15961 ctttacaaat ctaaaccaaa gtcgtagcaa tacggctttt tattgtgtcc aaactttgtt
    16021 gatgacacta aaagctacac tgtttcgtcg caggacgtaa agctagacta tcggttggtg
    16081 gcgtaaccac taggagaaag ttatggcaga agaaaatgta acaacagaga caactgagca
    16141 agtcgacact caaaaagaag ctgttgaata tcctaagcat gaatatgagc gaacttttac
    16201 tcgcgcagac atctcaaaaa tgatggctgc tgaacgcact aaatgggaat ctgaacaatc
    16261 cgcagctatt gaaaaagctc gtacagaagg tgaacgtttg gccaagttgt caaaagatga
    16321 gcgcgctaaa gaggaggaac aaaaacgtct agatgctatc gcagagcgtg aaaaagcagt
    16381 agcagagcgt gagatgcgca ttgagacgca ttccctgctt gtggagaaag gattgccatt
    16441 ggattttatt gatattgttt tagccactac tgcagaagag gttaaggcca atattgataa
    16501 tttacaaact atttttgata aagctgttga aaaacgtgtt aacgatcgtt taactcagaa
    16561 accgccacga actggaaatg gctcggtcgg tatgactaag gctgacatca tggcgataga
    16621 ggatgacgac gaacgtatgc gtttaattgc tgagaatcgt aacttatttt aagaggggaa
    16681 tattatggct gaaaaaaact taaacactat ggcggactta ggagatatta aatcaattga
    16741 ttttgttaac aagttttcca aaaatattaa tgatttatta aaattgctag gggttactcg
    16801 tcgtgaaaca ttaactaacg atctaaaaat tcagacctat aagtgggaag tgactttaga
    16861 tcaaactgat cctggagaag gggaaacaat ccctctgtct aaggttactc gaactaaaga
    16921 taaagactat acagtgaagt ggttcaagaa acgtcgtgca actacagctg aggcaattgc
    16981 tcgccatggt gcagctcgtg ctattactga agcagataag cgcattatgc gtgagcttca
    17041 gaatggaatt aaggatgcat tctttacatt cctcaaaaca aaaccaacaa aagttaaagg
    17101 cgttggcctt caaaaagcgc tgtctgcatc atgggctaag ctagctactt ttaatgagtt
    17161 tgagggttcc ccgcttgttt cttttgtctc tcctttagac gtagctaact atcttggaga
    17221 tactaaagta ggtgcggatg cctctaatgt ttttggaatg acattgctta agaacttttt
    17281 gggtatgcaa aatgtgattg ttatgccatc tgtgccagag ggtaaaatct actcaacggc
    17341 tgtagaaaat ctagtttttg catctttaaa tgttaaaggg ggagacttgg gcggcttgtt
    17401 tgctgatttt actgatgaga caggtttaat tgctgcagct cgtaatcgtc agctttctaa
    17461 tcttacctat gaatctgttt tctttggggc gaatgtactg tttgctgaaa ttcctgaagg
    17521 ggttgtagag gcaacaatcg aagctgctgc tgtacccggc attggtggtt aaaggctatt
    17581 tatatgggtg ataaacaact tattgacgat atcaaactct ttataggtat ttccaagggt
    17641 gatggtgcgc aagatgagct catcaccctt gctatatatg aaagtaaaga gcgtgtgcta
    17701 gctaaactta atgaatactc agaaactgaa atcaccaaaa ttcctgatag attgaggttt
    17761 attgtccgtg atgttgctat taaacggttt aataggatta attcagaagg agccgttgaa
    17821 gatagcgaag aaggaaagac ttttaagtgg gacagttacc ttaaagagta tgaatcaaca
    17881 ctcagaagcg ctgctattgg gaaggtatat tcaggcaaag gggtagcaag atttatttag
    17941 gagatacaca atgatctata aagatagagt aatcttagtg tatgtcgatg aacaggacga
    18001 ttttctagat aaaagaactg ttgaaaaacc tagcgggaaa atcccctgta tggaaaatac
    18061 tttcacaaaa tctgaacaga tggggctatt tggtaaatat gatttgaatg cttttaagtt
    18121 gcacttgcaa ggccattacg acggctttag caagattatc tacaagggaa aatcgaggtt
    18181 gataaaggga ctagcacacc ataaaaatag cacggttatt tatgtatgag tcttatttat
    18241 cggatgagag gcctagatag gtttttacgc agcgttgagc gcaagcagaa gtcagtacga
    18301 atcgctgtag ataaagagct tagtaaatca gctgctagga ttgagagaca ggctaaaata
    18361 ctagccccgg ttgacactgg atggctgaga gctcaaatct acagtgagca acaacgactc
    18421 ttacactata gagtggtttc tcctgcttta tattctattt atcttgaatt aggtactcgt
    18481 aaaatggaag cacaatcgtt tttagaccct gccctgagaa aagaatggcc tgtgctaatg
    18541 gctaatatca aaaaaatgtt taagaggtga tgcatggatt actcactgga aacactatat
    18601 ttaaaaaagg taaaaaatag attgggagtt ttagacatac caatctattt taaattgcct
    18661 aaatcagacg ttttagagcc ttttattgtt gtaggtacaa atatatcaga cttgtcaaaa
    18721 acagctcaaa ctggagcagt tattgatgat tttagcctga atatcgatgc tttcttacct
    18781 ggcgatagtc gtttggatgc agaagagata aaatctcgca tgcttagact gcttgggcga
    18841 aataaccaaa taaaggctca gattttggta gataattcaa taggacggga agtctataga
    18901 gttgctatca acattacaga aacactattt taaaggggac ctaaatggca aacacaaaaa
    18961 aaggaacaac tattgaaatt acaacaggta aaccgattgt aggaaaaaag attttttact
    19021 ttatacaatc agttgatgca ccaaagaaaa gtcaggctct gttgccagcc tatcgtaccg
    19081 atggtactac aaccatgggt ggtgaataca ttgatgagca aacccaacaa ggtcgcgtta
    19141 ttgagaaggc tacagatgaa cactctattg atttgacaac gtactttgta ccgacagatc
    19201 catctgttgc tgtcatcgag gaagctaaga aaacaggtaa atctatcaaa atttgggaag
    19261 ttattgccga cgaaagtgtc aaagaacaga ttcaaattcc tgaatcgact ggacccaaga
    19321 aagatgttta ccctgctaag tttgggtacg ccaagattga cgaaatcgaa cgcggtactg
    19381 gtatttcaga cttagtagaa atgtcctaca ccgctaacat tgtaggggcc ttacaagatg
    19441 gtaaattccc tctcacgaaa gaagaaattg aaatgctaga aaatgtatac ggctatcaaa
    19501 atccgggtga tactactggc gactacgata atatcactaa atagcacaca ggggtggcaa
    19561 ctgatacccc attttgattt aaaaaggaga taaaataatg gaatttacag cagcaaaacg
    19621 aaatatcgat attaaatttg attttaaaac tatgtttaag atcaataata aactaggaac
    19681 aattaaccca gaaacaggag agcgcaatgc agatggtgtt ggtgctttgt ttttcaatat
    19741 cttagagcgt aatgaaagcg ccattgttga ccttgtgcgt ttatctgcgg gaagcggaaa
    19801 aaaagcgcta actgaagatg aaattctaga tgcaattgca gaatctgttg atgaagaagg
    19861 aacaacagaa gggttgtttg ctgagattga aaaagaaatg gttgattctg gttttttcag
    19921 agcgaagatt ttgaaatata tcgaaaacat ggagaaatca gctcgttatc tcaaagcgaa
    19981 ggacgatatg gacgcaactc aaatccaaat tattgaagac atgattggaa gaatgagcaa
    20041 cgcagtatct tagtaaattg cgcacggcta gggctcacag acattgatac tatctataaa
    20101 tgtactaagt gggagctaga ggctattatg gaggggcttg agtataagca gcttgctgag
    20161 cgcgagagcc tctctgaatt gtctctaaag cttagatata cattaaatgc taaaaaggtt
    20221 gatgtaggta agctcaaata cgataagcat aggctaacta tcaaaagatc gtatcaaaaa
    20281 gctagccgta gccaagccga tagtgatagc agtattgttg agagaataca aatgcttaat
    20341 aatcattttc aaaatagata gataaggagg agtagatgcc aggaacattt gatggttcta
    20401 ttttcgctga tgttggtgcc aacacaaagg actatgagca agccatggct cgtattgtta
    20461 gtacgactca gaatgctttc agaaaagccc aagatacagc ggttaacagt agtaataaaa
    20521 tggttcaaat cattggacaa atcatggccc aactggcgaa caacggcgaa tcgctcggga
    20581 agagacttgg ctctgcctac gctactggtt taaaactgag catcggtgaa attcaacgca
    20641 tagcagcttc cattggcgaa aaaattcccg aacccataaa gaatggtttt caaaaaatca
    20701 ttattccagt tgcaagtgtt actgacaaaa tacggggtaa cttaaccggt ttcattaatc
    20761 aaacaaaaat gcaacttcag aatcttgtgg gtattggcaa gataagatct gcatttgcca
    20821 aggctgcgac tggagttgat atcttaactc agagggcaag tagtaagctt aataacttat
    20881 ctgcaatatt cgaaacggtc gcaggcaagc ttccgcgacc attcggaaca gcgtttagaa
    20941 aaattgcctc atccgtttct accctgaata gctctataca gtctgttgga gggaagattt
    21001 ctaattcact tggtcagcaa gtcttgaacc cagccttaca gtcatggaat aattttttta
    21061 gttctgtatc agctaaggcg tctgctttcg caaacaaagt gagtactagt ttgtttggtc
    21121 ggctgacctc aagtttagct aatttatcaa gtaaaatagg gagcagtctt agcaatggtt
    21181 ttagcagaat gtctagttct gcggctactt ccttgaatgg aataagccag aaattcgcca
    21241 atacctcttc tgctggagag agactcaaaa gtacagtgat gagcatcgtg caggccttta
    21301 gtttaatggc tgttgctcaa aaagctatgc acgctattac tggggcaata gacggggcgg
    21361 ttagtcgcgt tgataccatg aaccgctttc cgaaaacgat ggcgctattt gggtattctg
    21421 ctgaacaatc taaggcatca attgataagc tatcaaaagg aattgaaggt ctgccaactc
    21481 ctctagacag tgctgtaaaa agcgctcaac agctcgccat aaccacagga agtttagata
    21541 aagggactag tttagccctc gccttcaata acgcaatgat cggttacggg gcaacaactg
    21601 aaggggctga gcaagcactc aggcagttta gccagtcgtt ggggtctgga aaaattcaag
    21661 ctgaagagtt taactctgta tcagaagctg cgccaggttt aatgtctaaa atggcggaag
    21721 ccttcggttt tggtaaaaat ggcgtacaag atttaaaatc agccttatct gatggcaaaa
    21781 tcactgcaca agagttcgca gataaaatga ttgagcttaa cgatgcccaa ggcggatttg
    21841 cagagatggc ccaatcatcg gctggtggaa tccgaactgc atggaagaac gtacataccg
    21901 ccgtcgtaaa aggcgtagca ggtatgattt cagcctttga tgaagcggct aaagctaatg
    21961 gcatgaagac cattgctgaa acacttctta gtctgaagcc agcaataacc agtgtttttg
    22021 atacgattaa ttctcttatt ccaaatgcag ttgcagcatt cgctaggcta aaacagtcta
    22081 tcaatattga ctttagccca ttaggcgcca gcgttaaaga ggtgtttgct cttattaata
    22141 ctgttcttag agattttgca catactggtg aattatcagg gcaggctttt gataatctaa
    22201 aagcaaaaat cacttcttta gcccctaaag tcattgccct ttgggcagtg atgaaccccg
    22261 ctagcgctat atcaacgata atgcctttgc tttctctatt cggaaaagtt gctctagctt
    22321 taggaagctt agggacttct gtcggggcgt ttggcggcat aatttctagt gggatagcta
    22381 gtgccagcgg cgttgttggt gcctttgcgg caactctaag cggattacct ggcgtttttg
    22441 ctaccgcagc agggcgtggg ctatctgtgc ttggaactat gacaagcgca atgtccagtc
    22501 ttgtgagttt agcactggcg gccataggac cggccgctat tctaggtctt gtggtggcag
    22561 gacttggttt gattaatagt cagtttggcg cacaaatcga gcagctactt aacaccgcag
    22621 ttacaaaagg tcctggtatt attcaaggcc ttgtaaaagg aattacttct aaaataccag
    22681 ttttgatagc tagtggcact caattgattg ctaaatttgc aaatgctatc acagtgttat
    22741 taccagttat aattcaagct ggcgttcagc taattacgag tctcgtccaa ggtattggac
    22801 aaaatgcaac gaatttaatt agttcagcaa taaaaatcat tggtagtttt gttagttcaa
    22861 ttgcgagtgc attgccgcag cttatttctg ttggaatgga gctattatta aatgttgtca
    22921 acggcatagt tcaaaatata cctcttatta tccagcaagc ccagcaaatt attgatagtt
    22981 ttgggaatag tttacaggct aaccttccta gtattattag caacggtata gctattttgg
    23041 tgaatcttgt acagggaatt actcaaatgt tgccaactgt tctgcagata gctactcaag
    23101 tcattacgag ttttgtatct ggaattgtac agtttttacc gcagttactt caaggtggca
    23161 ttcaaatcat tattagcctt gtgcagggga ttattcaaaa tctaccccag attgcacaat
    23221 ctgctgtaca gattatacag tttttagctt ctggactgac gcaagctcta cctcagatta
    23281 tcgcagcagg cattcagctt gttgcgcaac tagccgtagc cttgataaag ggacttccac
    23341 agattatttc tgcgggtatt caattaatta tggggctcgg taaagccatg ttaggggcta
    23401 tcccaaacgc tctttcagga gtttgggaag gtattaagag cggattcagt tcgatgtggg
    23461 accaaatcac aggtaagagt tccacaagta cagctaaagt ctctgcggac gccacggtca
    23521 tggccatgaa tgttgggatg caaacgacag ctatagctaa tcaagctaat acagatacga
    23581 catctatgct taatagcatt agtcaaaata cagagcttgc taatataaac gctacatcaa
    23641 atgccacaca gatggctaat aacgttaatg ctcagacagg aacgatgagt cttcgggggt
    23701 tgaatgattc gatgtctctt gcgagtggca ttagcgctaa tatgtctgtc gctaatataa
    23761 acgctactac tcaggctcga gaaatggctt caggggttaa tggtgcgact tcttcaatga
    23821 atctagacgc cattaatcat acgctcagct tagctagtgg tgttggtgcg aacatgggag
    23881 ctgctagcac aggtgcaact tctcaggcgc aagctatgag ctcaggggtg agtaacagcc
    23941 tggcatcaat gcagtcaaac tcaactaaag cagcctctgg tctgtctaat agtgtgacaa
    24001 gcgaaatgtc ttctgcagca acctctgcaa cctcaagcgt taataggttg tcctcggcgg
    24061 tcgagtctgg cttcaataag gcgaagactt cagcaacaac ctcaatgaat gggatagcca
    24121 atgcggttaa aacaggattt agtagcatta acagtactgc aaagcaatct atggcaaacc
    24181 ttgtaagttc agtgacttct gggatgtctc gtgcaaccgc ggtgtctaat aatgcttgtc
    24241 agaagatcct ctcaatattc agggctttgg cagggcagat gtcttctgca ggagcttatg
    24301 caggtcaagg ttttgctaat ggtctagcta gtagcgcagg cacaatctac gcaatagcta
    24361 acagcattgc cgctaatgta gcagcaacaa ttagacgcgc tttagatatt cactcaccat
    24421 ctcgtgtcac taaaacacta ggtgcattta ctggagaggg gtttgccctc ggtatggcag
    24481 agtggatagg tgagatcaat agcctaggta aagcatacgc aacagcagtt actgatcaaa
    24541 actggggggt aaatagtact gtttcaacat ctgctaaagt taataatagt ggaatcaata
    24601 cctctcttga taatcttagc gaagaggtta ggcagtctca attgtcagag cctatctttg
    24661 aagtgcataa tgagattgtt ggagataaaa tttacacggc tgttaaagaa aaagaatcaa
    24721 gagagcaatc taaagattct tattttgttt ttgactagaa gggagtattt tggatttatt
    24781 aatagaaaaa gagagtcagg caactaggtt gtctgacttt ggtatttata atattgctat
    24841 tgaagatagc gctcctttac tatctgtatc acaccgagca gtcaaaggaa ggagtggtta
    24901 catctacgat ggggctactt ttacgactaa aacgcttaag gtcaaaggaa gagtgacgat
    24961 aagcaacgta gaaagacttt tggacaaaca ggacgaacta aacgctctgc ttgttgttga
    25021 cgaacctttc tacgtgacta agatgtaccc tgaaaactca gatctattta actttgagct
    25081 tccaggggaa agcacaggag acctgcaact tattggctct cctcataaac cttggaaata
    25141 tagatttaaa gtcatcttag acgacactat cgcttatgaa tttataggaa aaaccagtca
    25201 aggactaaaa tataatcttt ccttcgcgct aagaactgct gaattacctt tcggtgagac
    25261 aaagccaaag gacataactc tatcaggggg cagttttgcc tatgcaggaa ccgctaaagc
    25321 aagtcagcta gagtggcctt ttattataga gcttactcct tctggaggtc agactaattt
    25381 ttatattgaa attgatggca ggcgctttga gtttaagcag aatagccagc tacaaaatag
    25441 tgataaatta cttttgacag gcatagctac cacgctaaac ggaaattatg tcaatgctaa
    25501 aacgaactat gagtatttta tcttcaatcc taatcctaac aagagaatta cttacaaaac
    25561 ggattttctt ggcacgatta ggattttaaa ttttgtagag ttgtataaat agggaggtct
    25621 aatgattact tttttggatc atcgtgacat tgagtatggg gtcattagtg tcattaggca
    25681 tactaatgct gttaatggtg agcgctcagt gagcggggaa atttatacaa acagtgatgt
    25741 cctcaataat atcgatagag ggtggcgact tagatttgaa gatgagtatt acgttgttat
    25801 ttacgcaaag cctgttgatg ttggtcaaaa aacgcaagtt tcgtttgatg ccatacacca
    25861 atttttttgg gacttcagca agagtagtat ctatgagggt ctcggagatg ggtctcatac
    25921 cattgatacc tatctagaga cagtatttaa aggtagtggg taccgctata agctagaagt
    25981 aggtgtgaac gcatttagaa aacaatcttt taattataag tccaggttag atttattcaa
    26041 tgagattatt aaagcgaccg gacttgaatt ttcagtcagc gggaaagttg ttcggctact
    26101 aaaaaacata ggtactgatc tgtcaacagt ggtacgaaaa aatttcaaca tgaatgacct
    26161 taccattgaa aaaaatattg atagctttat tacctatcag aaaggctttg gtgcatggac
    26221 cgacccagag gaccactcta aggggagact tgaggcggaa tacgaaagtc ctcttgccaa
    26281 ggagtacggt cgacttgaag gggcgcctct gacggatgag cgcttcacgg tggcagataa
    26341 tctaaaagaa gctttaaaaa gtaatgttga aaattcatat aagatatcag taaaaattga
    26401 tatggaagat ttaacgagag ctgggtatag atgtgagagg ccggttgctg gtgactacat
    26461 tatggcgata aatgagactt taggttttca agagcgtata cggatagttt cttttacaag
    26521 ctactacgac gcaactggag ctttagtaaa acatgaagtc acttgtaatg atatcggatc
    26581 tgtgaaaaaa caaagcgtag gaagtctgtc tataaacagt agaatcaatc agatagatgc
    26641 agatattgct tctgcaattg aagtagcaac acaagcacta gtatctgctg acgggaaaaa
    26701 tactgtttac ggtggcacag aaatgcccaa agatgaacca aaaggaacct taaaaaaagg
    26761 agacatatta ttcctaaaag ttggcgatac tactagaatg tatttctgga atggtgctga
    26821 gtgggaagaa cctgaggttg tgaacgaccc agaacgatgg cgtgaagacc tcgaaaaaca
    26881 aatctctgag gcgatcgaaa aagccaaaaa agcacaggaa gaaatcaacc aacgcaccga
    26941 taaagagctt gaagaattta gagccgccct caaaaaccta gcgttaccag aggaagcgat
    27001 taaaaaaatc accgaggcta taaaagttga tgacatcccg tctattaaac aaagctttga
    27061 tgacctcaaa aatagagtga gtgagacaag cgaagaatct cgtttaactg ccgaaatttt
    27121 aggaaataac ggtaagaccc gctataacaa aaatttattg gttggcgacc ctaaccgtgt
    27181 aaaaacctat gaccaagact atatcgaggt agaagctaat gatggcggct ttaagcgtgg
    27241 cgagacctac acgattagct ttagtcaaac gtgtgagcta ctcaaagaag tagctatcac
    27301 attgacgcaa attaacaaca agggagttaa gttagtactg acaccaacca aagcaaaaat
    27361 ggatgcacag acgtttgagg tcactaagga taaacagtct atagaggtat atcctttaag
    27421 ttatacaggc gttttaactg gtgactggta taaacctaag caaatagatt taaacgcctc
    27481 agacacgcag gaattggctc tagagatgga ttataaagat gttgttgacg gcaaggatgc
    27541 cacaataaca ggggcatggt cagacagtcc acaaataatt ttatacggag gtaaaaaatg
    27601 acagaaacta taccattaag agtccaattt aagcggatga aagccgccga gtgggcaagt
    27661 agtgatgtcg tcttactgga gggtgagatt ggctttgaga ctgacactgg ttttgctaag
    27721 tttggcgatg gtcaaaacac ttttagtaag cttaagtacc ttactggtcc caaaggtcct
    27781 aaaggagaca ctggtctcca aggtaaaact ggaggaactg gtcctcgggg ccctgctggc
    27841 aagcctggaa cgacagatta tgatcaactc caaaataaac cagatctagg tgcgtttgca
    27901 caaaaagaag aaactaatag taaaatcacc aaattagaat caagcaaagc agataaaaac
    27961 gctgtttact taaaagcaga gtcaaaaata gagcttgaca agaaattaaa tctcaaaggc
    28021 ggcgtcatga caggtcaact acagtttaaa cctaataaaa gtggtattaa accctcatct
    28081 tccgtaggag gagcgattaa cattgatatg tctaaatcgg aaggtgctgg tgttgttgtc
    28141 tattctaaca atgataccag tgatgggccg ttaatgagct tgcggacggg taaagagacc
    28201 ttcaatcaat cagcgctttt tgtcgattac agcggtaaga ctaatgccgt taatattgcg
    28261 atgcgtcagc caaccacccc caatttttcc tctgcgctta atattactag cggcaatgaa
    28321 aatggtagtg cgatgcaaat tagaggcgtc gaaaaagcat tgggaacgct caaaatcaca
    28381 cacgaaaacc caagcgttga ggcaaattac gatgaaaacg ctgcagcgtt atctattgat
    28441 atcgttaaaa aacagaaagg cggaaaaggt actgctgctc aaggaatcta cattaactca
    28501 acctcaggaa cgacagggaa gttgcttagg attagaaatc ttggtgatga taagttctac
    28561 gtcaagtctg acggtggttt ttatgccaag aaaacttcgc agattgatgg caacctgaaa
    28621 ctcaaggacc ccacagcgaa tgatcatgcg gcaaccaaag cttatgtaga taaagcaatt
    28681 tctgagttaa aaaaactcat actaaaaaaa tagattaagg aggataaatg agcagagacc
    28741 caacacttat tttagacgag tcaaacctcg ttattggtaa ggatggacgt gtgcattaca
    28801 catttaccgc agaggacgac aacccaaaag tcagactagc tagcaagtgt ctaggcacag
    28861 cgcattttaa tcagctcatg attgagcgag gagaccaagc tactagctat gtagcaccag
    28921 tagtagttga gggtacaggt aatccgactg gactatttaa agacctcaag gagattagtc
    28981 tagagctgac agacatcgaa aattccaagt tatggtcaaa aatcaaggta aacaaccgag
    29041 gtatgttgca ggagtactac gacggcacga ttaagactga gattatcaac tccgcacaag
    29101 gtgtggctac acgcatcagt gaggacactg gcaaaaagct agcacttatc aatgacacta
    29161 tcaacggtat aaggcgagag tatcaaaacg ctgataaaaa gctatcagct agctatcaag
    29221 ctggcataga cggcttaaaa accacaatgg ccaatgataa aatcggttta caagctgaga
    29281 ttaaagcctc agcacaaggg ctatcgcaaa agtatgatga tgagttgcgc aagttatctg
    29341 ctaagatcac aacaacctca agcggcacga ccgaggccta cgaaaacaaa ctcaatggct
    29401 tacgtgctga gtttactcgc tcaaatcaag gtatgcggac agaactcgag tctaaaatca
    29461 gcggactaag aacggcgcaa cagaccactg ctaaccaaat ctcacaagag atacgtaacc
    29521 gcgaaggtgc tgtcagccgt gtgcaacagg acttagctag ctatcagcgg cgattgcagg
    29581 acgcagagga taattacagt agcttaaccc atacaattag aggtttgcag agtgatgtgg
    29641 ggtccccgac tggtaaaatc caatcacgcc ttactcaact agcaggacaa attgagcagc
    29701 gggttactag agatggtgtc atgagtatta ttagtggcgc tggagacagc attaaattag
    29761 ctatccaaaa ggctggcggc attaatgcca aaatgtctgg taatgagatt atctcagcaa
    29821 ttaacctcaa ctcctacgga gtaacaatcg caggtaaaca tatcgctctc gatggcaata
    29881 cgactgtcaa cggtaccttt accacaaaga tagcagaggc tatcaagatt agggctgatc
    29941 agattattgc aggcacgatt gacgctgcta ggattagagt gattaacctt aacgcaagta
    30001 gtatcgttgg tttagacgct aactttatca aagctaaaat tggctatgct atcactgatt
    30061 tgctcgaggg taaggtcatt aaggctcgta atggagcgat gcttatcgac ttaaatacag
    30121 ctaagatgga ctttaatagc gatgccacaa ttaattttaa tagcaaaaac aatgccttag
    30181 tacgtaaaga tggcacacat actgcctttg tacattttag taatgcgacg ccaaaaggct
    30241 atagaggctc agcgttgtat gcctctatcg gcattacctc atcaggagat ggcatcgaca
    30301 gcgcttcgtc tggacgtttc tgtggagtta ggtttttccg gtacgc
//
LOCUS       DAUCWO010000014        28170 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUCWO010000014 DAUCWO010000000
VERSION     DAUCWO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28170
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.014"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..208
                     /locus_tag="VGY20_000596"
     CDS             <1..208
                     /locus_tag="VGY20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273472.1"
                     /translation="MIATAAVVGLSLATPENQTVSANEAVATQACNYDFYGYCGPISW
                     LFYKTKHLLMGEQPTTTSTATVTK"
     gene            complement(489..875)
                     /locus_tag="VGY20_000597"
     CDS             complement(489..875)
                     /locus_tag="VGY20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273473.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLVARPVMADEEQTPEDRAYSQ
                     GLEKGTEAGRKAGEEATWTDLTPTVPTDLETPDDIEDTNKQLYKEGYTDGYKEGYNEG
                     FGEHYPLLIPVQFIWGIILHWWEMLS"
     gene            complement(1129..2844)
                     /gene="slo"
                     /locus_tag="VGY20_000598"
     CDS             complement(1129..2844)
                     /gene="slo"
                     /locus_tag="VGY20_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ4273474.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKAESSELTIEKADQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKDLQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(2868..3353)
                     /locus_tag="VGY20_000599"
     CDS             complement(2868..3353)
                     /locus_tag="VGY20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ4273475.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDFKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(3358..4713)
                     /gene="nga"
                     /locus_tag="VGY20_000600"
     CDS             complement(3358..4713)
                     /gene="nga"
                     /locus_tag="VGY20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ4273476.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYQYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     RYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            complement(5085..5624)
                     /gene="nusG"
                     /locus_tag="VGY20_000601"
     CDS             complement(5085..5624)
                     /gene="nusG"
                     /locus_tag="VGY20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ4273477.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(5797..6840)
                     /locus_tag="VGY20_000602"
     CDS             complement(5797..6840)
                     /locus_tag="VGY20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ4273478.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVVKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYVEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            complement(7167..8459)
                     /locus_tag="VGY20_000603"
     CDS             complement(7167..8459)
                     /locus_tag="VGY20_000603"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ4273479.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            8897..9775
                     /locus_tag="VGY20_000604"
     CDS             8897..9775
                     /locus_tag="VGY20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273480.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKVAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            9788..11050
                     /locus_tag="VGY20_000605"
     CDS             9788..11050
                     /locus_tag="VGY20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ4273481.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDNM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSTTPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIVNANNAALQMLAETSKEAIPA
                     LEQSAQSPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(11170..11796)
                     /locus_tag="VGY20_000606"
     CDS             complement(11170..11796)
                     /locus_tag="VGY20_000606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ4273482.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTLEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(11841..13256)
                     /locus_tag="VGY20_000607"
     CDS             complement(11841..13256)
                     /locus_tag="VGY20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ4273483.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(13257..15032)
                     /locus_tag="VGY20_000608"
     CDS             complement(13257..15032)
                     /locus_tag="VGY20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ4273484.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHTGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(15233..15553)
                     /locus_tag="VGY20_000609"
     CDS             complement(15233..15553)
                     /locus_tag="VGY20_000609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ4273485.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVLPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(15550..16548)
                     /locus_tag="VGY20_000610"
     CDS             complement(15550..16548)
                     /locus_tag="VGY20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ4273486.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(16564..17148)
                     /locus_tag="VGY20_000611"
     CDS             complement(16564..17148)
                     /locus_tag="VGY20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ4273487.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(17217..17696)
                     /locus_tag="VGY20_000612"
     CDS             complement(17217..17696)
                     /locus_tag="VGY20_000612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ4273488.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(17698..19698)
                     /locus_tag="VGY20_000613"
     CDS             complement(17698..19698)
                     /locus_tag="VGY20_000613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ4273489.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEESHRQQAAQTTLKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQYKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEVDSVNQLKGLMTETLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(19688..20008)
                     /locus_tag="VGY20_000614"
     CDS             complement(19688..20008)
                     /locus_tag="VGY20_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273490.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(20463..21482)
                     /locus_tag="VGY20_000615"
     CDS             complement(20463..21482)
                     /locus_tag="VGY20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ4273491.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGGACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(21519..21884)
                     /locus_tag="VGY20_000616"
     CDS             complement(21519..21884)
                     /locus_tag="VGY20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ4273492.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            22058..22738
                     /locus_tag="VGY20_000617"
     CDS             22058..22738
                     /locus_tag="VGY20_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4273493.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(22859..23506)
                     /locus_tag="VGY20_000618"
     CDS             complement(22859..23506)
                     /locus_tag="VGY20_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ4273494.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(23509..24168)
                     /locus_tag="VGY20_000619"
     CDS             complement(23509..24168)
                     /locus_tag="VGY20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ4273495.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLILICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(24180..25364)
                     /locus_tag="VGY20_000620"
     CDS             complement(24180..25364)
                     /locus_tag="VGY20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ4273496.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            25485..26395
                     /locus_tag="VGY20_000621"
                     /pseudo
     CDS             25485..26395
                     /locus_tag="VGY20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(26465..27871)
                     /locus_tag="VGY20_000622"
     CDS             complement(26465..27871)
                     /locus_tag="VGY20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ4273497.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKRIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         8539 a         6032 c         4932 g         8667 t
ORIGIN      
        1 tatgatcgca actgctgctg ttgtaggcct tagccttgca acaccagaaa accaaactgt
       61 ttcagcaaat gaggcagtag ctactcaagc atgtaattac gatttttacg gatattgcgg
      121 acctatcagc tggttattct ataagacaaa acacttgttg atgggagagc agcctactac
      181 tacttctacg gctacagtca caaaatagtg agacaacagt tataaaaaag cccaggactg
      241 cgtaagcagg cttgggcttt tttgctgtcc taaagttagg gtgttgccac aacttcaaac
      301 ccaaagacac ttaaaccgca ccccaacaat tagacatagg ctatgtcccc agaatcctgg
      361 acacatatca ctaaagccta atgattaagg tgcggtttaa ggtgtggtag caaggaactg
      421 ttttaaattc gtctgcgtct aactataccc actagctatg gagtcagctt agagttgatt
      481 gtcaaatgtc agctcaacat ttcccaccaa tgtaaaatga ttccccatat aaactggaca
      541 ggaattaaca aagggtagtg ttctccaaat ccttcattat aaccctcttt gtacccatct
      601 gtataccctt ctttataaag ctgtttatta gtatcttcaa tatcatcagg tgtttctaga
      661 tcagttggga cagttggggt caaatcagtc caagtagctt cttctccagc ttttctccct
      721 gcttctgtac ctttttctaa accttgacta taagctctat cctcaggagt ttgctcctca
      781 tctgccataa caggtcttgc tacaagcaac atagcagcaa atagagccac caagaggtag
      841 acgaagcgta gaggctggta agattgttgt ttcatgataa tagttatctc tcccttttat
      901 gttatgcttt gcttgttaaa aagcagcttg ttagtattat agattagaag cagcttagct
      961 tcaaggtcac gatgtgagaa aagaataatt ttttgattag tatataaaaa agcttgtcta
     1021 tgaaggtatc tcataaacaa gcttttttct gtatttgtga gaggagtagg cacaaggcct
     1081 caagagataa gcagcatcaa gctgtttgac gaacctcttg aaccagtcct atttataagt
     1141 aattgaacca tatgggctca gggttgatcc tgagatgttg acattgattt ctttagacag
     1201 tttcacatct ctttcgtcga tcacttttcg ccaccattcc caagctaagc cggtgcactc
     1261 tctagccatg atacggatat ttcgtgaatt agctcctagt gggataactg tgctaaatgg
     1321 tgatgtctta ctataccagt tgttgtccca acgtcgtttt gtaatcactt cttttccttt
     1381 gtcatcataa ttgatttcat cccaaaggat ttcatattga gcaacatacg cgccttgatg
     1441 agacaggtta atttttccac tagtgtactc ggtagatgtt gtttcaacgt attcagttct
     1501 gttattgaca cccgcaattt tattattttt aaggaaaaca ctggtgtatg aaataggata
     1561 agctgggttt tttctactga aggtagcatt gtctttgata acgtttctaa taacatcaaa
     1621 gtcttttgtg actaccttat tgtgctctgc agcatctcct cctaaaacga cagctgtaaa
     1681 tgaactattt tctaagatat cagagtattt tccattcgtt ttaacatctg ttccttttag
     1741 agctgcacta aaggccgctt caacatcatt acttttagaa cttgtttcta gtttgacaaa
     1801 aacagttcga ccataggcta cgttactcac aaagagtgga ggagcttcat tgctgacacc
     1861 ttttcgttgc aaatctttaa aggtcactga tttatcaaac acatccgcag gattattagg
     1921 aaggtttgct gatacggtgt aaaaaatttg cttgtatgct gcaatcatca ccttcttttc
     1981 accttttgaa atcgacttga aatcaatgcc taaagtacca tctaagattt tgctattaac
     2041 atttagagct gcttcaatct gtgacttaga atataccatt gattcagtat attgtgttct
     2101 ggcaggaagc gtattaccac cagaataatt atcatgccat tggttaacaa gattatcaat
     2161 agctgttgaa acattggcat aggtagggtc attgacctca accgttgctt tgtctcccat
     2221 acctggtaaa tcaatatgga ttttttgtgg gtttcgcttg gtgactaccg cgtctggttt
     2281 gttttcggta aaacctttat cagccagctg aagggctgct ggataggtca tatcagtgac
     2341 agagtcaatg atggaaatat cgactggtgt agtgttgata ttttttttct ttctttcaat
     2401 gacaataaat ttatcagctt tcttaacgcc ttctttagga acaaaatttt caatggtttc
     2461 accattttta gcaagtactt caagctcatt ataatttagt gaataaatct tgtcattgat
     2521 ttcttcagtg tgatcttctt cgctcttttt tttgtcttct gacttttttt cttctttttc
     2581 tgcagattct agtggcattt ctttgggagc aagcttaatc atatcgttag agttaagcat
     2641 atcatccatt ttctgatctg ctttttcgat agttagctca ctactttctg cttttggttg
     2701 ctcatttgtc gttgtggttt ctgtactagc agtgttttgt ttgttcgatt cagcattagc
     2761 agtaacaaga ttaccaatga taagagctgc cgttagtagc ccagcgacgc gactgtattt
     2821 tttaaatgtt tttttattag acatgtcctt catacctttt tatcattcta aaatgtttct
     2881 attgttcttt cgaccatatc aagcatagta tcttgatagt gagcaaattc tcctaatttg
     2941 taatgtgcca tagcctctct aatatgcgca tcttttaacg ctttggttat attagctagt
     3001 atgctttgac ggtttaaata aaatggcaag ctttcgtaat aatttaataa catgttttca
     3061 gcgtttaggg tttgaaccgc ttggtaaatg tctaatgctt gaccgctatt tgacatttct
     3121 aataatgctt cttcgatatt agctttcaat tgggtatagc cttttatgaa agcagggtaa
     3181 accccatact ctaaaataac ttctggatca gaaatatctc ccatataaga gtctcttcta
     3241 gtgatacgat actctttgtc actacctatc aagtattttt taaaatcatt aacagttact
     3301 tctttttgaa acattttttg gtaatgttct aaaccctttg gcaccttata catattgtta
     3361 cttcctatct tgcattctat cttgcatttt cttaattttg aagcgaccag ttactttttt
     3421 atggtcacca tttggagcaa tgatggtagg atcaataatt tcaaaatagc ctttggcatc
     3481 aatctctgct aagacagctt tagattttag atctttaaca ctaatcgatg ggaaaattaa
     3541 ttcactttct gaattttcaa tgtgttttcc tgaccacgtt tggcaaccag gaatgttgag
     3601 cgaatttgct ccaggggtat ctccgctagc tgttcggata tagcctagct tatcaatgtt
     3661 ttttgttagt tcagaatcgt actcatattg ataaacccca tcgctatatc ttccgctatc
     3721 gacacctttt atattttcaa tttggtcagc tgattttaac ttaccttgat cagtaaagag
     3781 caatgccata tcttcagcaa aacttgtcac aaaaagaccg ttttcttgtc gaccaatcgt
     3841 tgactttctt ttagcatcat caaaagctaa catcaaatct tttttgcttg taacgtggga
     3901 agctccgctt tctttgtaga cttgttttag tttttcaaca aactcttttt tgtactggta
     3961 tttgcggcta acatacatcc ggtaatactc agcacgtgat gcaacagcag acatctgaac
     4021 agctttatca gcatacttgt cggcagtttc aataaactca tctgcttgtt tatctgttac
     4081 ccatttatct ttaatttctt taaacttttt ttcaaaaaga tcgcgttcca tattgccaaa
     4141 agtgacctct gacaaggcta aaatatcctt gttttgtccc tgatggacct ctgttacctc
     4201 aatatcaata cgatcaacat ctaatttttt atcaatctca atgtgatgcg gttttttagt
     4261 gtaggtgtcg cctaattcaa ttttttgaaa gaggaccaat ttattcccgt tatagtattt
     4321 aacatcagcc tttgcaattc tatttttctt gtagtaattc tccttgctag atgtatctcc
     4381 attaacaatt aaaattctcc caatacgcat cggggaagca aacttgtagg aaagtacctc
     4441 accaacgcca ccaccaggtg aattttcctc ccagacagta ctcattgagc cgtctaatgt
     4501 gtgcatgaca tgattgtctt ctttagagga agttgcgtta gcttccataa ctttggtcgt
     4561 ttcatatttg acatcgcttt ttttattttc tttgccacta acagcgtatg ttgtgctatt
     4621 agctaataaa ctgctaccca ttgctccagc caaagcaata gcaacacttg tctttgcgac
     4681 aatatgagct aatgttactt ttttgtttct catgtaaacc accttatatt atttttaagt
     4741 aaactataca ttaacaaata gtctttgtca atgcggttta ttaacatcgt tttaggcttt
     4801 tctatcaaaa aaatgaacaa aaaaaaaaca agtagccaac tgttacaagt ttcaagggga
     4861 tatttccatc ttattatcac agaaccttca catctgttct atttaaacca gattaaaagt
     4921 actagttaaa tatttgatag tagcaccgtt atcagtaata gatctgttaa ttatctctga
     4981 ttacaatgat gttaacaata gtcatacctt tgtttgtaca cacgcttcac tgccaactaa
     5041 aaaaggtctg atggtaatat ttataccatc agacctttga gagcctatag ctctgcaatt
     5101 tgataaagtt caacttctgc tactgtttca gagccaaaca tgttgagcat cagcttcact
     5161 ttgttgtttt cgatttctac cacacgacct tcttgtccca taaaggcacc gtcgatgatc
     5221 tgaaccacat cgccttcttt gatattggta tcaaagacat caatggtttg acccatagac
     5281 aacaagatag cacggatttc ttcttcaaga agtggtgttg gttttgatcg gttcccgtgt
     5341 gatcccacaa aacctgtaac gtttggtgtg ttacgcacaa caaaccaagc ttcatctgtc
     5401 atgaccattt caaccaagac ataccctgga aagcgatttt cttcgatttc tttggtttgc
     5461 ccattttttt caacgttaac cgtttgtgtc ggaatttcga cgcgcaaaat attgtccaac
     5521 atgttatagg tttgggcacg ttgcaagagg ttttctttta ctttgttttc gtagcctgag
     5581 taagtttgga ggacgaacca gcccttatca aatgaatcta gcatgatgtt tcctttcatt
     5641 ataaaaaagc cctttttttg gcttttgcgc tgttttctat caccttcatt ataataaaag
     5701 acagggccgc tgtcaacgta tctgtggagc tacttgacac ctttattaaa aagtccaaag
     5761 tcggttcgtg acgactctgg acggtattgt acagttttag ttttcaggaa ctgtgatggt
     5821 tccttcgata atcgcttttt tagcagtctc aatagctttt ttagtgtctt gtgtcagagc
     5881 atccgttgta agactaacac caccttcttt taaaccaaaa gtggttattt gaccacctgg
     5941 gaattggcct tctgaggttt ttacggctac ttttactaaa gcttttccga cttccttgat
     6001 acttgaggtc aaaacaaaat tagctgactt gccatctttt gtggtgtatt ttccatcttc
     6061 actttggtca cggtcaacac caataaccca aaccttatct tcttctttac gtttttcgtt
     6121 gatagactta gcttcgctaa agacacccgc ccctgtgcct cctgctgcat gataaataac
     6181 atctgcgcct tcaacgtatt gagcagctgc aatcgtcttg cctttggcag catctgcaaa
     6241 agagcctgca taagcaactc ttactttgat ggtatcatct actgatttca caccagcttc
     6301 aaaacctttt tcaaagcgct tgacaacatc tccttccata ccacctacaa aaccaacttg
     6361 cttggttttt gtcgttttag ctgctgcaac accggctagg tatgccgctt catggtctga
     6421 aaaggtgata cttgcaacat ttttttgacc tttaaccaca tcatccacaa ttgcaaaatg
     6481 gttgtcagga ttgtttgcgg ctactttttc tacagcgtca tgtaatggaa atccaatccc
     6541 aaaaacaaga ttataaccat tggttactgc tgactcaagg tttgtggtaa actctgattc
     6601 attggctgat tggaaataat catagccttt tcctttttca agtttatttt cttttcccca
     6661 agcttgtaag ccttcccagg ctgactggtt aaatgacttg tcatcaatac ccgtctgatt
     6721 agtaatcata gcgattttta atgaattgtc agttgtttgt ttagcagagt tattggtaca
     6781 gcctcctaat gtgaataggg cagtcgaaac aagaccaagt gacattactt ttttgttcat
     6841 gtgaaaatga acctcctaaa tgtttaatcc taaagtgatg tgtcattgtt aacgttctac
     6901 ttatgctcta gaacgaaaag tttagtatat caaaaaaatc agttttatcc tactattttt
     6961 gctgaaaaat ctctttctca ccaaaatgac cgaacgataa agtgagatca actggtaaaa
     7021 cccattatct cctcaaaaat cacctcttaa ggagataaga caacgacaaa actcaagcaa
     7081 ctagtaatag catgactaac tatacaatcc aacaaacggt aaaaagagct ggaaacgcta
     7141 tccaactctt tgtcatacta gacaccttag atagatgccc agactgactc aagaatattg
     7201 gtctgttcac gccctggacc aactgagaag gttgaaatgc gaacgccaac caattctcca
     7261 acacgacgaa cgtagttgcg ggcattttca ggaagctcat caaggctacg aacacctgtg
     7321 atgtcctctt gccagcctgg taattcttca tagattggtt tgcaacgttt gagttgttca
     7381 aggcttgctg ggtaatagtc aatacgtttc ccatcaaggt cataagccac acaaatctta
     7441 accgtatcaa gccctgaaag gacatcaatt gaattcagag agaggttagt aatccctgac
     7501 acacgacgac tatgacgcat aacaactgag tcaaaccaac ccacgcgacg tggacggccg
     7561 gtcgttgtcc cgtactcatg acccacttca cggatacgtt cacccacttc atcaaagagt
     7621 tctgtaggga atggcccatc accgacacgg cttgtgtagg ctttacagac accaactact
     7681 ttgttgattt tacttgggcc aacaccagaa ccaatggtta caccaccagc aactgggttt
     7741 gaagacgtta caaatgggta cgttccttgg tcaatatcaa gcataactcc ttgagcacct
     7801 tcaaaaagaa cacgtttacc ggcatcaagt gcatcattta aaatcacaga agtatctgtc
     7861 acatattgct tgatttcttg accgtaagca tagtattctt caaaaatagc atcaaaatca
     7921 agaggtgtac tgtcatacat tttttcaaat aaacggtttt tctcagctaa attaatgcgt
     7981 aggcgctctg caaagatatc cttatccagt aaatctgcga tacggatacc aactcgagca
     8041 gctttatcca tataagctgg accaatccct ttaatagttg ttccgatttt attgtcacct
     8101 tttgcatctt cttgcagttg atccagctgg atatgataag gtaaaatgac atgagctcgg
     8161 tctgaaatgc gaaggttatc tgtcgtcact ccttcatcat gcaggtacgc caactctttg
     8221 accaaagatt tggggttgac cacaacacca ttaccaataa cggaaatttt ttgcgggaag
     8281 aaaattcctg atgggatcaa gtgaagttta aacttcttac catcaatcac gatcgtatga
     8341 ccagcattat caccaccttg atagcgagca attacttctg catccgctga caagaaatca
     8401 gtgattttac ctttaccttc gtcaccccac tgggtaccaa caacaactac agatgtcata
     8461 tcattgtcct aactttctga aacacttatt ctcaataaaa atcataggca agctaaccac
     8521 tgcttaaaaa ccaaaaagaa ggtcagatct gtaagatcag caatgtagct cttgatattt
     8581 aaagaaaaca ggcaggaatc tcacctgcta gcatatatct tacaacttat tatagcaaaa
     8641 aatggttttt tctgctagat ttcttagcat tattaaggaa aaagagaaac tcttttttta
     8701 accatgataa acaatcctaa aaaaacgaac attataagag aaaagctttt aacctcgttt
     8761 ctcctaagct ttagcagggt taaacgattc tcttaaaaca ttagctcttt tctttcagaa
     8821 atagcggttt taaggtaaaa taatagtaag ttacctctat gagatagcca gtaaaaacat
     8881 atcgaggaaa tatcacatga ttagaaagta tgaccgcacg agcacaaaaa agaaatcact
     8941 aaactggatt tggctaatca ttgctttttt tatgatttca agctttatag gtggctcttc
     9001 ttttactgag tcacttctag acattttgcc tgccatagcc attggtggta caggttatgc
     9061 tatttttcgt gttagatctc atcaaaagcg gctagcaaag gccaaggttg ccaaacaatt
     9121 agaagatcta aaagcaaaaa ttcagctagc tgatcgtaaa gttcggctgt tagatactta
     9181 tttggcagac catgatgact tccaatataa cgtactagct cagcaattat taccacaact
     9241 tagtgacatc aaagcaaaag ctattacgtt aaaagaccag ttagatccca aaatttatcg
     9301 tcgtatcacc aaaaaagcca atgacgttga aagtgatatt acccttcaac tcgaaacact
     9361 gcagattgcc acaactctta accctcagcc tttgaaaacc ccatctccta atcttattaa
     9421 caaagcacct gagctcaaac cgtattatga taatattcag acagatcatc aagctatcct
     9481 tgctaaaatc caaggagccg acaatcaaga agaattgctt gcgctgcatg aagctaacat
     9541 gaggaggttt gaggatatct taacaggcta cttaaaaatc aaagaagagc ctaaaaatta
     9601 ctataacgct gctgctcgtt tagaacaagc caaacaagct atccaacaat ttgatgaaga
     9661 tcttgacgaa acccttcgtc gactcaatga aagtgatctt aaagattttg atattagcct
     9721 tcgaatcatg caaggtgcta ctcaaagacg aacaacgcat caccaaaaag actaaaggag
     9781 tccctttatg gcagatttta attttgacat tgaccaaatt gctgacaatg ctgttgtcaa
     9841 aaccgataaa accaccgata ttatctcaga cttaccaact gacacaaatg gccaaatctc
     9901 tttttttgag aaactaagtg ctgaccagca gacagctatt actgccaaag ccccggcatt
     9961 ggtagatacc tttttagcag atcaaaatgc gctacttgat tttggtcagt ctgctgttga
    10021 aggggtcaat gcaacggtca atcatatcct ggccgaacaa aagaagttac agattcctca
    10081 ggttgatgat cttttaaaat caaccaatcg tgaactcaat ggttttatcg ctaaatacaa
    10141 ggatgccaca cctgtggacc ttgataaaaa gccaaacttt cttcaaaaac tctttaaaca
    10201 aagccgtgat accctgcaag agttttattt tgattctcaa aacattgagc aaaaaatgga
    10261 taatatggct gcagcggttg tcaagcaaga agatacctta gctcgcaata ttgtctcagc
    10321 agaattgtta attgaagata atactaagtc cattgaacat ttggtaggcg tcattgcttt
    10381 tatcgaagct agtcaaaaag aagctagtca gcgcgctgcg gctttacaaa aagacctaaa
    10441 aaccaaagac agtactacac cagactatca aataaaagct gatcttttgg caagaactac
    10501 cgaagtcatc aacaccctcg aacaacaaca caccgaatac ttgagtcgcc tctatgttgc
    10561 ttgggcgaca actccgcaaa tgcgtaatct agtcaaggtc tcttctgaca tgcgtcaaaa
    10621 actaggcatg ttacgccgca ataccattcc aacgatgaaa ctatctattg ctcagttagg
    10681 catgatgcaa caatcggtta aatctggaat gactgctgac gccattgtca atgctaataa
    10741 tgctgccctt cagatgctag cagaaacaag taaagaagcc atcccagcat tggaacaatc
    10801 agcacaaagc cctactttgt ctatgaaatc tgtgacaagt ctggcagaaa gtttagtagc
    10861 acaaaataat ggcatcattg cggcgattga tcatggacgc aaagaacgag cacaactaga
    10921 aagtgctatt atcaggtctg ccgaaaccat taacgattct gtcaaactac gtgaccaaaa
    10981 tattgtgcaa gcacttctta gtgaaggcaa ggaaactcaa aaaacaattg ataaaacaac
    11041 tcacgcataa gctaaacatc acctgtcacg ctacttaaaa agcaagccat cagttaggtt
    11101 aatccctttg atggcttgct tttttactac ttaacgaata atcgccttgt ttgatataac
    11161 acttatcttt tatgatgtta ttttcataat acgaacaagg ctagcacgct cggcctcttc
    11221 taatttcaac tcaatgtagt gaatcgtctc ttccagttgc gggataatag aatactctaa
    11281 gccattgacg cgacgtctag tcttttcaat gtcatcagcc attagttgac aagttttttc
    11341 aacttcagct aaccgtaaga gcttttctaa tagttctttt gtcttttgga tagtattgtc
    11401 catctcacta tttgagctga ggtaactata agcaaactca ccttgttctt gctctcttgc
    11461 cgtattagac tggacatgga atttaggcac attgacactc atgatatttt caatatcaat
    11521 ccataaggtc acttcatgga ctggaacagc aaagagttct tctaccatta gactattttc
    11581 agatgctttt gccaagacaa actccttcat attggcagca agttcttttt cgagagtttg
    11641 acgaagctca ttgttttcac gaattaaatc aacgaagcgg cgcatcaact catcccgctt
    11701 gtcctttaat aatttatgac ctcttgtagc tgtcttcaat cgatttttca agttactaag
    11761 ttccatgcgt gttggtttaa catttaatcg tgccattgat ataactcctt tcgtcaaggg
    11821 cttgaaattg catggtcaac tcaatcattt gcaacaaaaa cctttgtcat cgtggtatct
    11881 gctttaggca aataacgatc taacatatca tctttaatgc gttttaattc ggtacgtggc
    11941 aaaatcgaca acaattccca acccaagtca agactttctt caatgctacg gttggtgtaa
    12001 aatccttggt taatgtattc ttcttcaaac cggttggtaa aggccacata tagtttatcc
    12061 gtttcagaaa gggctgattc accgagcaca acagccaatt ccttagcttg ttttccttgg
    12121 gcataggctg caaagagctg attcattgtg gcagcatggt cttgtcgagt cttaccttca
    12181 cctgaacctt tatcttttag gcgagatagc gatggcaaga cattaatagg cggtctaaaa
    12241 ccatttttat atagttcttg cgacaaaata atctgacctt cggtaatata acctgtcaaa
    12301 tcggggatag ggtgggtaat gtcatcttct ggcatggtta aaataggaat ttgagtcact
    12361 gacccttttt tcccgatcaa gcgaccagcc cgttcatata aggtggagag attagtatag
    12421 agatagccag gataacctcg tctgcctgga acttctctgc gagcggctga tacttctcgt
    12481 aaggcttcac aataatttgt catatctgtc atgatgacta aaacatgcat acctttttca
    12541 taagccaagt attcggctgt tgtcagagct atacgtggtg ttgcaatgcg ttcaatagca
    12601 gggtcattag ctaaattaat aaataaaaca gatcgatcaa tggcacccgt ttcacgcaag
    12661 tcattcataa agaattctgc ctcttcaaaa gtaataccca tagctgcaaa cacaaccgca
    12721 aagttatcat cagagtttag aacagttgcc tgacgagcaa tctgtgcggc caattcatta
    12781 tgaggtaacc ctgagcccga aaaaactggt aacttttgac cacgaacgag ggtattaagg
    12841 tgatcaatag ctgagatacc tgtctggata aattcgtctg ggtaatcacg agcgacagga
    12901 ttgatcgctt gaccatcaat atcaaggtat ttttcgggaa taagttctgg accgccgtct
    12961 atgggctgcc ccattccatc aaagatgcgc cctaccatat cttctgatac ggctaattct
    13021 aaaggatgcc ctgtaaatcg aaccttggtt ttggctaggt tgatgccagt tgatccttca
    13081 aatagctgta ccatagcctt gtcaccttgg acttctaaga cttgaccttt acgcctttct
    13141 ccattatgca aagtaatatc taccaattcg ttgtagtgca ctcctgcaac ttgatcaaca
    13201 atcatcaaag ggccaaccac ttcgctaacc gtccgatatt ctttgagaac gctcatctaa
    13261 attcctccct ttgctaaaac gtgatgaatc tctttggtaa ccttctgagt aagcccttta
    13321 atctgctcta agttttcttc tgggataaat ttgctgcgtg cgatgcgatc acgtacctga
    13381 gcagtgcctt ccataatctc attaaaataa gctcccaaag aaagggcttt gctggcttcc
    13441 tcattaaagg tcaaaatatt ggttagcatg gcctcttgtt tcggaaagga agtaaaggta
    13501 tccaccgaat caaaggcatt ttgctggaga taatcctccc gaatttgccg ggcaacagcc
    13561 atggtcaaac gatcttgttc agacagtgaa tcaagcccca ccaagcgtac aatttcttct
    13621 agactggctt cacgctgcaa aatagccatg gcacgagtta ccttgttgga ccaattagat
    13681 tgctgtttac ggtcaatata gcttcctaca tcatcttgat acaatgaata agacgtcagc
    13741 cagttaatcg ctgggaagtg acgccgttgc gcaagaggcg cgtcgagccc ccagaaaact
    13801 ttgacaatcc gaagggtgtt ttgagtgaca ggctctgaaa tatcccctcc aggaggagaa
    13861 accgcgccga tggctgtaat ggttccctca cgttcttgac ttcccaaggt ccgaacacga
    13921 ccagcccgtt cataatattc ggcaatacga ctccctaagt aagccgggta gccttcatca
    13981 ccaggcattt cttgtaggcg tcctgacatc tcgcgcagag cttctgccca acgtgatgtc
    14041 gagtctgcca tgatagccac agaatagccc atatcacgga aatattcggc aatggtaata
    14101 cctgtgtaaa tcgacgcttc acgcgctgct actggcatat tagaggtgtt tgcaattaac
    14161 accgtgcgct ccataatgga ctggcctgta tttgggtcaa ttaactctgg aaactcattc
    14221 aaaacgtcgg tcatctcgtt gccgcgttcc ccacaaccga cataaataac aatatcaacg
    14281 ttggcaaatt tagctatttg atgctgcaca actgtttttc ctgccccaaa tggtccagga
    14341 acggcagcgg caccaccttt tgtaacaggg aaaaaggtgt caataacccg ttgacctgta
    14401 accaaaggtt cgactgggat aagcttttga gcaacagggc gactttgacg aactggccaa
    14461 gtctgcatga ggctaccttg gtaaatggaa ccgtcttcct gcttaatttc ataaactgta
    14521 tctgttactg tgaagtcccc tgtatgaata gccaccaagg tccctgaaac tttataagga
    14581 accatgatac ggtgctcaat gacagctgtt tcttgcacag ttcctaaaat atcacctgca
    14641 actacttctt gaccaacact tagcttggga ataaatgccc acttagcctt tcgatcaaga
    14701 cttgggatag ccacaccacg gattaaaaag tcgctgtccg ttgctttttg aaaacgatca
    14761 agcggtcgct gaataccgtc aaacatttct gaaatcaggc ctgggcctaa ctcgaccgac
    14821 aaaggacaac cagtagtcac tactggttct cctggaccga tccctgatgt ttcctcataa
    14881 acctgaatag acgcttgatc gcggcgcatt tcaataattt ctccgactaa gccaagatgt
    14941 cccacacgac aaatatcttg aatattagct tcttgcatcc cagaagccac aacaagaggt
    15001 cccgaaacgg ttattatttt tccttggttc acctttttcc tcctttattt ttttcaaatc
    15061 atactaacta atctatactg tcaaggttta atgtgactat tttgaatagt cagcaaacta
    15121 tcctatgcgt tcagctgcta acttcaccaa ttttcctgtc cttgcatttc atcagttagc
    15181 tactcataag cctcaaacag agtataagtc ctctttgcat aacgtcttag tgttataaaa
    15241 tattttgacc gacagccttt tcaaccatgg cttgtatgcg tgacaggcct atcccttgcg
    15301 ctccttgatg agttggtaat ggtaggactg ctggtagaac ttgattgtca tagtgagtga
    15361 gggcttctgg aatagctgca gcaatatctt ctgtgatata aataatacca aagtcttcca
    15421 ttgctagttg acgcaactgg ttaatagcgt cttgtggttt tattactgga aaagtttgaa
    15481 acccaatcat ctgaaaaggt agaataacat ctctgttacc aattaccccg actttataca
    15541 atgttttggt catgctattg gtctcatcct ttctatgaca cgttctttgg gtaaatcatt
    15601 agctaaagca gccgctaaaa gccgtaggtt ttttacttca aacgatttag cgagcaaaaa
    15661 gcgagcaagc acataaggtc catcgacttg atatttggcc ttcgcaaata aatggtaaag
    15721 gcattcatca acaaggtatt ctaactcagt cgtttgaaga gtgccttggc gaagtttctc
    15781 tgaataaggt tttagggctg tcatatagct atcaggtgtt acattttcaa accacgtcat
    15841 cagatcctta tcatcttcaa gtgtgataaa tgttttactt ggcctacttg cttcatcaga
    15901 taataactgc atcatgaaac ttattggttt attttgcgac ttggcccgtc tgacggtaat
    15961 cagattatac aaatcaatca caataagaac agcttgttgg aaaacagggt cctctaatct
    16021 ttgtgctatt tgtttcaacg ccttaaaata agctaagtca gtaccaattt ctaagacgcg
    16081 tatgtcttga taatcttgat attcggccca aatggactgg atttctgtta ctacctcttt
    16141 tggaaactca tcagaagtca tcgtcctaat caaatgttcc aaagccacca gaggcttgtc
    16201 accaattggt agtaataagt gactcagatc tgcttgacta gctttagctt ttaacaacac
    16261 tttgacatta tgataggtgt aacgcaaggt aaataattgt actatgtcag gttgaggggt
    16321 ttctgcaaaa gcccagcgat aggtcttggt tagctcagcc attaagatag attctagccg
    16381 atccaaatcg ttgagatcgt caacagttag gtgatagact gactggtgca acagtcgagc
    16441 taaggttgtt gtattgggag cctgcaatag cttatcaaat tgctctttag tcaataactc
    16501 tttttctttg acactgatag tagtgtttaa ttctagaaat gttggcatcg ctagtcaaac
    16561 ctcctatatc tctgaaaaaa gcatatcaat gatgcgggag ctttcttctt taagaactga
    16621 ttctaaaagg taacggtata aataattgtc atcgacttgg tctaaactga ctaaaaatcc
    16681 tgcttcctga ggaattagct cttgattata gcttaattga ggaaacgcta atctcagctc
    16741 agcacaatca taagaactga acttttgccc tgttttttcg ccaaaagtaa cttgcaatgg
    16801 ctgttctgga tatttagaca agacttgctt caaaaaagcc aattcttctt ccttagaaaa
    16861 gttcgtcatc ttctccaaag aagcttcaaa tagctccttt atactgtctt gttttaatgc
    16921 tagtagagct tgtctttctt gatttttgag ttgctgctgc gcaacctgaa attgctgttc
    16981 aagagctttt aactcttttt gacgctttgc ttttaaatcg tgtagtcctt gctgttgacg
    17041 ctctttaaag tcagtatcaa gactatctgt cgctatcttt aggcactgtt gaccttcttg
    17101 atgtgctttt tcaagaacgt tttgtcttaa ctgtgttata tcattcacct aatttccccc
    17161 ttttgatagt gttagtggtg tcaccgtttt ttaaaatcta gataaggacc gttaccttat
    17221 ccaattttat ttaagagaat aaaggagacg acaaaggcaa gaatagcata ggtttcaacc
    17281 atagctgcta aaatgacacc cttcataaaa tctttagggc gcttagccaa gatttgcata
    17341 ccagctacag aaacattgcc ttggtgcttg gccgaaaaat aaccaacaat agcaattgga
    17401 agagatacca aaaagtaagc taatccttgg ttaactgaaa gttctggtgt cagtttcatc
    17461 caaattaaaa taccaatagc aaagccgtag attccttgac taccgggtaa cagttgcaag
    17521 ataagcgcag acgtaaattt ttcgggttct tcttttaaga gcgcagctgc cgcttgacca
    17581 cctttaccga caccataagc tgagcccatg ccactaagag ctacggctaa aacaattcct
    17641 aaggctgcga agaaaacgcc accatgtgct gtaaaataat gtgctaaatg ttccatgtta
    17701 attgtcctcc aaatctgttt cttcattgac attgacataa ttatcagcta actttaaagg
    17761 attgaaagct tttcctccac cttcataaaa ttttccaaaa aactctacga aaataagacg
    17821 agcaccatgc acatatcctg aaagcattga taagaaaata ttgatcgcat gcaaaagaat
    17881 aaaaataaag atacctactg taaagcgcgt gactggtgga aaaatcccaa caatcatgtt
    17941 aaaagccgct ccaatactag ctccagaaag ccctaatgcc attagacgag taaagctaac
    18001 caaatcactt aagtaagagc taatgccata taaattatat aatccactac ctagacctaa
    18061 aaggcttttg ctttttagta aagaaacaac caaaatccca aaggcattgc ccaatgcaag
    18121 ccacttacca ataaccgata agtaagcaag gccagataca ttcttaccaa gaataaataa
    18181 cagcaggcct aataaaataa aagtccaagc tagacttgac gtataggccg tagcatatgc
    18241 tttcatcctt acttgttgcc atgctcctaa caaaagccca aatataagtg taacaaaccc
    18301 aaatagcaaa gaaacaacca aaatagtaat cacatccgtt ttagttgata ataatgccac
    18361 aggtaagtca aaaccaaaga aagaaccgta aataagcccc catagggcta ctgaaatagc
    18421 taaaatatta aagaaagtca ctaaccgttt actggttttt tggagattaa aaaagaccaa
    18481 tgcagctagt gtcacagcat atagcaacaa accatagccc aaatcagcta ccatcatgcc
    18541 aaaaaaagta aggtataaag gagctaaaaa aggtgtcgga tctttttctt gatattttgg
    18601 caaagcgtac atttctgtaa ccaattcaaa cggtgcaatg tagcggtgat tacgtaactt
    18661 aatgggaaca tcttcccaat cgtctggcgt gacatcgtaa ctatccagat aaaacatatc
    18721 acctagtgtc tcagtcatta gcccttttaa ttggttaacc gaatcaactt ctatccaccc
    18781 ttctaaggct attagatgtc ttgtaccgag taattgcttt ttagtttgct gccgttgata
    18841 ctgactaagg acatagtcta tttcaacctg taactgtgcc agtatctttt gagactgcct
    18901 aagctctttt agtagactat ctttttcagc tagccaattc gttagtaact ctttgtttat
    18961 acgaagttgt tctgatggca gtagctgttc tttatataat aagggtttaa actgatattt
    19021 ctgtaaatga atcgtatctt gtgcttgcca aaataagaca aggccgtact caaattctgt
    19081 atggtatact tcctcaataa aaagaccttt gtactgcttt agctgatgat aaaaatgatc
    19141 atttgctgta ctaggaatcg ttccaactct tgccgacaaa aaatgaaatt gcgctaagtc
    19201 ctgaggcaaa acagtcaagg aacgccactt ttctaactcc tgacagtatt ctgttagctg
    19261 gtctattttg ttttgcagct gctctagtcg ctctatcttt tgtgacatca ttttcaaagt
    19321 cgtttgagct gcttgttgtc gatggctttc ttcgaactgt ttaaacgaaa gagatggcgt
    19381 tttttgtctc aaggctgtta actttcctat tggtggtaaa aacgctgata agctagcaag
    19441 gccattttct aactcttgtt ggctttgtaa aaggtattgt aaagcttcat catctagtag
    19501 aggtttttga tgcgtcagat cgtattggac gatttgtggc aacttgacgt tgcctttgtt
    19561 aaaagcatct tgccaatgtt ttagttgctt catgtcacga acttcaacta attgagattg
    19621 ttgaagtgtt tttaaaacaa gatcaagata atctttttca aaaaccattg ccagcttttt
    19681 catttgacta atggccatat gtctccacca ccttatccac taactcccta acaacggtta
    19741 gcttatgctc ttctaaaatg gcatgaaggg ctagctgttg cgtcgctaac ctcttttttg
    19801 aagctttttg ttcttgacga ttagcttcaa aaaacacctc acgtttagct tctaatagta
    19861 gctgagtttc ttgttcgtac tcaaggaaca gctgctttaa ctcttcttct tttttgtcag
    19921 ctagagcttt tttcttagca tcgtaactgt cataaattgt tctagcctct tgttcgatac
    19981 tattcattag tgacaatagg gtattcatca catatcaact ccttatcttt atctattgct
    20041 aattctttga ctcactaggt acttaaaaac aaagctacaa gaaaggctac ttttgggggg
    20101 ttaatcaacc agcgaatata agctagagct aaatctctaa cctatctact aacagctttt
    20161 ttgagatagg ataatagctt aattaaacga acagcatctg taagttaagc gctttcaatt
    20221 ctatccataa aaacaatggc ttagttcaga aaaaggttag ctaaaaacct gtcaaagcag
    20281 taagatgaca cagaactcta aagctttgta ttgctgtctt tcatgtgtta tcttatactt
    20341 ctttaggcat ttagtaacct tgatctcgtt gacggtaagg aacctttacc agccaatagc
    20401 caagccaatg tgataacagt ataactgcta tcgtgttaaa agtcctgtca aaaagatgtt
    20461 tattagtttc tcgcttgtaa ttcttgtttt gttactaaac ggtaattttt aaagacagaa
    20521 cctccaatat aacccacaat taagctgata ataatacatc caagggctgt tatcaacact
    20581 ttagcgacag gattataggc aaacatcacc gcaaaaccgg caatcggagt agctgtccct
    20641 ggcgtatcat taaccaattt cattagggcg atcaaaacac cacatgcacc accaccaaca
    20701 aaattagtca cataaatcgg aatgggattg gctgacgtca catctgcctg agtcaacggt
    20761 tcaatagcaa aagcaatatt gtcttttctt tcacccaatt ttaatcggta aaaaagaaca
    20821 ccattcataa aagaagagcc aaagacagat aaggcaccta tagccatcgg aatacctgtt
    20881 agaccaagca tggctgtcaa agccattgat gatagcggag ctgttgctac aacaacaatc
    20941 gtaccgccta gtatcattcc catgataata gggttgctat tagcacttga ggtcaggatg
    21001 tctccaatag tcaataaagt actattgatc actggcgtaa ttaactgcgc caagaaacga
    21061 gttaatggtg caccgacaat aataatcgag attaaatcaa gcccaccagg aatatttttt
    21121 tccatccact tgattaaaaa agataccaca tacccagcca caaaacctgg gataatacct
    21181 gttcctgaaa ctgacaaacc aatcaaaacg gcataacctg gagacacacc aattgcaatg
    21241 gcaaccaaga tagcagcagc tacccctgat aatgagccat ttgctccgcc tacttcactt
    21301 agaaactgaa agcctaaaac cttaccaaaa aaagaatctt gaaaggcttc aactaaaaaa
    21361 gaggcacaag ctgccgaagc caaagctccc attgccttgg caccatgagg agccttatag
    21421 ttaaacaagc taaaaatagc taaaacaaga atcaaaagac ttgttccaat aataatatcc
    21481 ataatctatt ccaccaatct atctcattac ataagacact ataactgctt cactctggca
    21541 acggcttcga cttcaatcaa agcttgcaaa ggaagcgccg acacttgaac cgctgtccga
    21601 acagggtaag gctcttcaaa caaatcagtc atgacatgat ttaatatttc cacattggtc
    21661 acatctgtta aatacacatt tagcttatag atatgattca agtccaactt ttgctctgcc
    21721 aaaatagatt gtaaattaac aaaaacctgg cgacactgtg cctcaaaacc atctgacaat
    21781 tgacctgtca ctggattaag gggtaattgg cctgcggtgt atagaaaatg tccttcaatc
    21841 gtataagttg aataagggcc cattggttct gggtagctct tcatatacga acctcctcat
    21901 tgataatata gttaaatttt acaggaaacc gcattcacag tcaataataa attctctttt
    21961 tgataaaaaa ttattattaa ggtgattggc aattgttttt tagtctaaaa tgtattacta
    22021 tatttgtaaa taagaagata gatataggaa gtttgacatg gataaagaaa cgctaaacta
    22081 ctggaaaacg gttatcacat ttcttcacga tgtcttaggt gataactatg aaattatttt
    22141 acatgtgatt gataaaaatg atatctatat tggagaactc gttaatagtc atatcagtgg
    22201 acggtctaaa cagtctcctc tcaccacctt tgctcttgat ttgattacca ataaagtcta
    22261 taaggaaaaa gattttgtga ccaattacaa agcaatcgtt agtcctcagc acaaagaagt
    22321 tcggggatct acttttttta ttaaagataa aaagggtaat ctcgagggaa tgctatgtat
    22381 taacttagat atttcagcct atcaaggagt agctagggac ctcttaaaac ttgtcaatct
    22441 caatttagaa catttcatcc caactgctaa agaacctaaa acagtcacac cccagccaga
    22501 agaagctgtt gaaattttaa cctctaacat ccaagatatt attggtcaaa ttattgatcc
    22561 ctcgttactg agacacaatg tacatcttag ccaagatgtc aaaatagaca ttgttgccaa
    22621 gctttatgaa aaaggagtct ttcaactaaa aggagccgtc tcaaaagtcg ctgatattct
    22681 atgtatttcc gaaccaagtg tttaccgcta ccttaaaaaa attgaagccg atcaataacc
    22741 aaatcaccat aaaacgccag ttaaaagtat ttcagataag catcacaatc tctgatatac
    22801 cgatgactga cgttttttta ctcgatatgc ctttatggca agaaaaggct actagtcatt
    22861 aaccttcagc tatgcttaga ggtacgcctg tcgcagcttg aacctcatca aaagtaaacc
    22921 cttcactgat ttcaattact tggataccat ctggcgttac tttaaacaca cccatttctg
    22981 tgataataag gtcaaccaca ccttttgctg ttaaaggtaa ggtacattct tttaggagct
    23041 tatgctttcc tttattggta tgctccatag ctacgatgac ttttttagca ccaactaata
    23101 aatccatcgc tcctcccata ccaggaacca ttttgcctgg aatcaggtaa ttggcgatat
    23161 ctccattttc tgcaatttct aaagcaccca agacggtcaa gtcaacatga ccaccacgta
    23221 taatgccaaa agaatctgca ctattaaaga aagcggctcc tggataaacc gtcacaggct
    23281 gtcctcctgc attaacgata gtcgaattcg gatcatctga cttaggacct agtccaataa
    23341 acccattttc agattgaaaa tacactgtca tttcttcagg cacaaaattt gctactttgg
    23401 ttggtagacc aatacctaaa tttaccaaag taccatcttc taattctttg gctacacgct
    23461 ttgcaatacg attttgaatc tcttctttac ttaatgcaac cgccataact atactccttc
    23521 tttaacaatg taatctacaa aaatgtgtgg tgtatgaacg cattctggtt gtatggtgcc
    23581 gactggtact atttctcgcg cctgaacaat tgttgttttt gcacaagctg ccatcaattg
    23641 attaaaatta ttttcagaac cactatactg taaatttccc atttcatcag ctttattagc
    23701 aaatatcaag gcgacatctg cttcaaatgg gtattctaag agataggttt ttcccttgat
    23761 ggtttttgtt tccttgcctt ctgccactaa ggttcctacc cctgttggcg tgtaaaagcc
    23821 accaatgcca taagctccga tgcgaatttt ttcggcgaag gttccttgag gaatgagctc
    23881 aatcgtcgtt tcaccggcag tcatttggcg cccagcttct ttatttaatc caatatgtgt
    23941 tgcataaatg gttttgaatt gatgattagc caccattttt ccaacaccgc gatcaacaaa
    24001 accagcatca ttacagatca agattaagtc ttttgtgcct ttgtcaacca gtgcatcaat
    24061 taacgcttct ggtgttccat tagccataaa accaccgacc ataatcgtgt caccatcttt
    24121 aatatgactg atagcttctg ctattgctat tcttttattc tctttcatac ggttaccttc
    24181 tatcttgtta cgatgatact ttgaccttgg ccaccaccaa tacaaagcgt tgctaagcca
    24241 tgtctcacat cacgcttagc catttctgct aataaggtga ctaaaatacg agcacctgat
    24301 gctccaatcg gatgacccaa agcaatagct cctccattga cattaacttt ttcattgtca
    24361 aaaccaaggt ctcgtgaaac cgctaaagcc tgagcagcaa aagcttcgtt tgcttcaatc
    24421 aaatcaatgt catcaattgt cagctgagcc tttgcaagag cctttttcgt agcaggtatt
    24481 ggtccgcagc ccataatact tgggtctaca cctgcacttg cataactagt gattttagct
    24541 aaaataggga gccctaattc ttcagctttt tctttactca tcaataaaat tgctgctgct
    24601 ccatcattga ttcctgaagc attaccagca gttactgtcc cctcatcttt gataaaagca
    24661 gggcgtaact ttgctaactt atccactgtc gttccaaatt tagggtattc atcttgatca
    24721 acgagtaaag gttcaccttt acgctgagga atagtgacag gtgcaatctc ttctttgaaa
    24781 cgaccttttt ctatggcaag ttgggcttta cgttggctat cagctgcaaa ggcatcttgt
    24841 tcttctctgg tcagtccata ctgttgcaca atattttcgg ctgttatacc catgtgataa
    24901 cctgcaaaag catcactcaa cccatcttta agcatggtat ctactacttt actatctccc
    24961 atacgagatc cccaacgttg gccttgtaaa acgtaaggtg cttgactcat gttttcggta
    25021 ccaccgacta aaacaatatc agcgttgccc gtttgaatct tttgagcacc taactcaact
    25081 gcttttaagc cagaaccaca gaccatatca atcgtaaaag caggggtatc ataagaaagc
    25141 cccgcattta gcgctacttg ccgagcaaca ttttgaccca gacctgcatg taacacattc
    25201 ccaaagatca cttcatcaat ggcatcactt ttaagaccgg tatcagctaa aatttttgta
    25261 acaactgtca cccccaaatc tacggctgat aaagatttaa agactcctcc aaaatttcct
    25321 atcggggtac gataagcaga tgtgatcacc acttctttag tcatagaaac ttctcctttt
    25381 tcattattga aacgcttaca tttttattat ataatgtgaa ctataataaa caaatatcgt
    25441 ttttttatat atgctataac taaacgttat cgaaagagga aaatatgaat atttcaagct
    25501 tggcttattt tattgagatt gttaattgtg actttaactt aaccaaagca gcccaaaaaa
    25561 gtcatatttc gcaacccgcc cttagtagtt atatcaagac tatcgaaaaa gaagaaggag
    25621 tcgttttatt ccagcgtcaa aaagggcgtc tagttggact aacctcagtc ggcgaacgct
    25681 tttttgaaaa tgctaaggtg gttatcgcat cacatgagca attaatggct gatttgcgga
    25741 gtgaatccaa aaaatacaaa ggagaaattt caatcggtat tcctcaactc atctctggca
    25801 ttgttttttc tgagctcatt ccaaaactca tcgaagaaaa tcccgaagtt aaatttcata
    25861 ttgtagaaac gggtgcttat gaactgcaaa aaaactgttt ctaaaagaag tcgattttgc
    25921 gattttatta acacccacta cactagatgc tactattgct gatatcgaca ttctcctaag
    25981 agatcaactc tgcctttata tgagtcacaa aacctttcaa gagacgtttg atagtaatcc
    26041 taacatctct atcaaagcat tacatcgtca aaacttagta acttttgata aaacctttat
    26101 gattaatcac cagcttacca ccttatttga agaagcaaat agtcttcctg tagtcaaatg
    26161 ttattctgcc tcgtgggatt ttttattaaa ttgcacgcgc tattcctctt acctaacaat
    26221 cctaccaagg cctattactc attttgctca tatggacggt ttagtggagg tccaactgac
    26281 cgaacatcca aaatgggaag ttgttctagc ctctctcaag cacaataaaa caagccattt
    26341 aaaacattat atcaagcaca ccatcttaga ttatttcatg aaccagcgct tctaattgct
    26401 tgactcagac cttaaaaaaa aacgcctgaa ggaaacaatc ccaagcgttt catatagtta
    26461 tttcctaaaa tacaaatagg taaatgatgg caatacctgt gaccaccaag acacaaggaa
    26521 tagtataagc cacaatttga ttgaatttta gtttgaagtt tgagaggtaa gataaggccc
    26581 aaaaaggaac tatcaagttt gttgtggcat ctcctaattg atacgcctgc aaaatcttac
    26641 ctaggtcatt accaacttcg atggttgcag ggacgatata gggagcttca atcacaaatt
    26701 ttgagcctcc tgatggtacc gcaatattca gcaaagccga gtaaagataa gcaaagaccg
    26761 gccatgttgt cccatttgaa atagcgacaa agaaatggga aatctctagt cctagacctg
    26821 tactttgaat gattccaaaa ataccagcgt aaaatggaaa ctgaagcacc aagccccaag
    26881 atgacatgac cccgtctttg aataaggaac cataatattc tggtccttgt tgaccacaaa
    26941 gaagcaaccc taagcctaaa aataaaaagt taaaactatt gagatctaga ccagacaagc
    27001 ccaccaccga aaaatgataa aggctatagc taacaatggc cccaccaaca agatacatca
    27061 ctaagcgaga tgcgttcatc ttctcagcag gtgtgtttct tgcaataaca acatgtgaag
    27121 cggtatcaag actatggtga tagatctcat catcaagttc catgatttta gtttcatctt
    27181 ttgggtgcgc caataaagca aagcaaagcg gtaccagcat acacaccact aaaagactga
    27241 taaatggaag attcaaaact gattctgtca gaggaacact ttcaggaaca acctgtttat
    27301 aagcatctgc gacactattt cgcaaataat ctggagtagc cgaataaagc accgcagcac
    27361 cagataaacc agaagtagct ggtaaaaagg taaaaaataa agtagcaaca aacaaaggtg
    27421 tatgcacctt aatccttttt tgcctagcct gaacaagcaa ctctttccca aacacaatgg
    27481 caaccatcat accaagaccc cagtgtaaaa agccaaagat agaacctact acaatagaaa
    27541 aaataaaaat ctgagtcctg gtttgaggca gtttggcaag ggacttaaac attttatgag
    27601 caggtggaga actcgctaca gcattccctg tcgcaagtaa aatagtcatc tgcatggcaa
    27661 aggacaacag cccccaaaaa ccatttaccc agccatagat aatcccgccc gtcttttccg
    27721 taccaataaa gggactatcc gtcatcaaca cagacatagt aacgactaaa aaggttaaaa
    27781 taaaacagat aaaaagcgat tctggcatcc atttcataaa gccatcaata tagcgatcca
    27841 taaacctttt tttggttttg atgttattca tgttgtcaat tcctttcaca ttgtccacaa
    27901 gcacttttgt tcttttgatc agtaacctat cgataatatt aataagcgtt tctttagttc
    27961 atttagttat caataagttt aaacccacaa aaaacacttt tgttcccttt ttattatgac
    28021 aacgttttca taaacaaaca actatgattt ttttattgct tccataaccc ttatttatag
    28081 caaaaagaat ggcgcttcac tcatcaataa tgtctttgtt atatttaaat aattaaataa
    28141 ttatgataaa aaattaaaaa ataatttact
//
LOCUS       DAUCWO010000015        28122 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUCWO010000015 DAUCWO010000000
VERSION     DAUCWO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28122
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.015"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..650)
                     /locus_tag="VGY20_000623"
     CDS             complement(<1..650)
                     /locus_tag="VGY20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ4273498.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKN
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQAD"
     gene            complement(767..1417)
                     /locus_tag="VGY20_000624"
     CDS             complement(767..1417)
                     /locus_tag="VGY20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ4273499.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(1414..1854)
                     /locus_tag="VGY20_000625"
     CDS             complement(1414..1854)
                     /locus_tag="VGY20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ4273500.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            2073..2921
                     /gene="ylqF"
                     /locus_tag="VGY20_000626"
     CDS             2073..2921
                     /gene="ylqF"
                     /locus_tag="VGY20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ4273501.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDAD"
     gene            2911..3702
                     /locus_tag="VGY20_000627"
     CDS             2911..3702
                     /locus_tag="VGY20_000627"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ4273502.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDTRLGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            3767..4603
                     /gene="dprA"
                     /locus_tag="VGY20_000628"
     CDS             3767..4603
                     /gene="dprA"
                     /locus_tag="VGY20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ4273503.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQKQQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIISGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVITEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            4709..6838
                     /gene="topA"
                     /locus_tag="VGY20_000629"
     CDS             4709..6838
                     /gene="topA"
                     /locus_tag="VGY20_000629"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ4273504.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEVFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFISNRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPEAIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMEEGENVKKVSTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEEWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(6913..7797)
                     /locus_tag="VGY20_000630"
                     /pseudo
     CDS             complement(6913..7797)
                     /locus_tag="VGY20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            7810..8376
                     /locus_tag="VGY20_000631"
                     /pseudo
     CDS             7810..8376
                     /locus_tag="VGY20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            8391..9383
                     /locus_tag="VGY20_000632"
     CDS             8391..9383
                     /locus_tag="VGY20_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ4273505.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESSLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWRLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARAALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYCTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            9488..10153
                     /locus_tag="VGY20_000633"
     CDS             9488..10153
                     /locus_tag="VGY20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ4273506.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            10146..10862
                     /locus_tag="VGY20_000634"
     CDS             10146..10862
                     /locus_tag="VGY20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ4273507.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            11013..12359
                     /gene="trmFO"
                     /locus_tag="VGY20_000635"
     CDS             11013..12359
                     /gene="trmFO"
                     /locus_tag="VGY20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ4273508.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            12512..13918
                     /locus_tag="VGY20_000636"
     CDS             12512..13918
                     /locus_tag="VGY20_000636"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEQ4273509.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLATVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            13961..14275
                     /locus_tag="VGY20_000637"
     CDS             13961..14275
                     /locus_tag="VGY20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273510.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            14275..14625
                     /locus_tag="VGY20_000638"
     CDS             14275..14625
                     /locus_tag="VGY20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEQ4273511.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSRPAAPATEKEMNANA
                     AGGGIQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            14635..15765
                     /locus_tag="VGY20_000639"
     CDS             14635..15765
                     /locus_tag="VGY20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ4273512.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKIMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKIGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(15965..16849)
                     /gene="citG"
                     /locus_tag="VGY20_000640"
     CDS             complement(15965..16849)
                     /gene="citG"
                     /locus_tag="VGY20_000640"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEQ4273513.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGRFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(16842..17537)
                     /locus_tag="VGY20_000641"
     CDS             complement(16842..17537)
                     /locus_tag="VGY20_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4273514.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(17695..19098)
                     /locus_tag="VGY20_000642"
     CDS             complement(17695..19098)
                     /locus_tag="VGY20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEQ4273515.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            19478..19801
                     /locus_tag="VGY20_000643"
     CDS             19478..19801
                     /locus_tag="VGY20_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273516.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSVIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            19858..20253
                     /locus_tag="VGY20_000644"
     CDS             19858..20253
                     /locus_tag="VGY20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ4273517.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVSVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VALSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            20298..21419
                     /locus_tag="VGY20_000645"
     CDS             20298..21419
                     /locus_tag="VGY20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ4273518.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            21607..21915
                     /gene="citD"
                     /locus_tag="VGY20_000646"
     CDS             21607..21915
                     /gene="citD"
                     /locus_tag="VGY20_000646"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEQ4273519.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEQTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            21903..22790
                     /gene="citE"
                     /locus_tag="VGY20_000647"
     CDS             21903..22790
                     /gene="citE"
                     /locus_tag="VGY20_000647"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEQ4273520.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGVLDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENGIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            22793..24325
                     /gene="citF"
                     /locus_tag="VGY20_000648"
     CDS             22793..24325
                     /gene="citF"
                     /locus_tag="VGY20_000648"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ4273521.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNIVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYAEQVVILTDNLVPYPNTPT
                     NIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            24318..24896
                     /gene="citX"
                     /locus_tag="VGY20_000649"
     CDS             24318..24896
                     /gene="citX"
                     /locus_tag="VGY20_000649"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEQ4273522.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            24911..26305
                     /locus_tag="VGY20_000650"
     CDS             24911..26305
                     /locus_tag="VGY20_000650"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ4273523.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVYTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YRETGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEVVITCRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(26415..27467)
                     /gene="citC"
                     /locus_tag="VGY20_000651"
     CDS             complement(26415..27467)
                     /gene="citC"
                     /locus_tag="VGY20_000651"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ4273524.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDTHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWELLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(27556..28038)
                     /locus_tag="VGY20_000652"
     CDS             complement(27556..28038)
                     /locus_tag="VGY20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ4273525.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLTVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
BASE COUNT         8717 a         5407 c         5713 g         8285 t
ORIGIN      
        1 tcgtcagctt gagcaccagg agaaaaagaa ggaaaaccag caatggtatt tgtcatcgtg
       61 attaagagaa acttcgtttt taggtgaata cgatggttgt ggctaattaa agagatggca
      121 tagctgcgat ttcgtttcag tattcgaata ctatctttta aaaatgctag aggaccaaag
      181 cgtcgttttt cctcagccgt tacgtcggca gcaatatcag ctagtaatcc taaggttaaa
      241 ctggaaatca tgtattgctg gttagctttg caaatatcaa cttgtttgat ttgtcccgtc
      301 aagatagtat ccaacgcctc agtaatttgt aaagggatat gcatagcctt ggcaaaatta
      361 ttgactgtcc ctgagggcac taagcctatt aaacaatgag ctcctccttc atagatgcca
      421 ccgatgattt tatttagtgt cccatcgcca ccaagaggga taaccaagtc aatcttgtcc
      481 ttagcagctt gttttgctaa ttgaaaagcc tggtcagcat ttttaggggt aataacttct
      541 acagaatctt cggaaaaacc atgttggcaa aaatagtctt tgacttgccc agcaagttga
      601 tcttctttct ttccagaatt aggattgtaa aaaatccgaa cggttttcat caggtctctc
      661 ctttgacagc ttattacttg ctatccatta taccacttct aagagttgat gtcttacatc
      721 aactcatcac attaagcctc tcaatcagtc tgagcatcta tttgtcttaa agcataaaga
      781 aagtgatagc gataaattgg agggcagagg ccaataaaat aaagagatgc caaatcatgt
      841 ggaagtaggg tctttttttg gcataaaata ccgctccaat ggtataagac aagccaccaa
      901 ataacatgag aagaccaaaa gcaagactag ttttttgaat gatgactggc aagataaaca
      961 caacgagcca gcccataacg atataaagca tcaaactgaa tttttcattg atacgtttgg
     1021 caaaaatttt gtataaaatc ccaaaaagcg taatgcccca ctgcaaaaca ataatgatat
     1081 agcctagcca accagaaaca agggataagg caacaggtgt ataacttcct gcgatagcaa
     1141 tgtaaatcat actgtgatca ataatgcgta gaatgtattt gtgaaccgaa ccataagcca
     1201 tggagtggta aatcgtagag gacaaaaaca tcaaaaagag actgatgaca aagatggaga
     1261 taccaatagc ggctttaagg tcataagttt gataagcata gctggctgaa ataggaagta
     1321 gaatgagcat agcaaatgct ccgatagcgt gtgtcacgct gtttgctact tcctcactaa
     1381 aagataaggg taaactttgt ttaaaggttt ggttcattgg gtcacctcct gtgataaata
     1441 aatagttttg tggtaatctt tgaccgtttg acaggcaacc tgcacaatcg gtggaatgtc
     1501 ctcctcaatg ggagccttct gattccaaca ggttacaaaa gtattgtaaa gactttcaga
     1561 atcagcctgt gcctgaagtt cctccaatac gcgactaata tcttgaatag gaaataccgt
     1621 tttaaacaaa ctgatggcaa ttaagcgcgc tagttgttgc ttttggtatt ttttcttgat
     1681 aggtttagtc acatacccat gcttgacgta attattaatc atagatgccg ttagtgattt
     1741 attatctgac acttctgaaa aatcagtgca ctggttgaca taaagtaaga cttgatccaa
     1801 atataagtct aaatcaggga ggtccttcca ataaggtaat gatggaaaaa tcatagtttt
     1861 gtctctctat ctagttttga taactagatt atacgattaa tgaaaactta ttgcaagcaa
     1921 aaactgtaaa gacttaaaag aggaaagtag aaatggcgaa gtgaagctaa tcatgctata
     1981 atgttaaaga attgattggg atagatgcct tctgtcgcaa tcagactatt ttatcacgaa
     2041 gaagatgatg ctacgaaagg aatgatacac tcatggcaat gattcaatgg tttcctgggc
     2101 acatgtccaa agccagaaga caggttcagg aaaatgtaaa gcatgttgac tttgtaacta
     2161 ttttagtgga tgcacgctta ccactttcta gtcaaaatcc catgcttaca aaaattgttg
     2221 gtgataaacc taaattaatg attttaaata aagctgactt ggcagatgca actcgaacta
     2281 aagagtggaa ggcttattat gaaagtcaag gtattaaaac attagccatc aattccaaag
     2341 aacaatcaac ggttaaaaaa gtaacagaag cagccaagga actcatggct gataaaattc
     2401 aacgcttgcg agagcggggc attcaaaaag aaaccttgcg aaccatgatt attggcattc
     2461 caaatgctgg taaatctacc ttaatgaatc gcttggctgg taagaaaatt gctgtagttg
     2521 gcaataaacc tggtgtgact aaaggacaac aatggttgaa gtccaataag gaattggaaa
     2581 ttctagacac tcctggtatt ttatggccaa aatttgagga cgaattggtg ggcttaaaat
     2641 tagctttaac aggtgctatc aaggaccaat tactaccaat ggatgaagtg actatctttg
     2701 gcttgaatta ctttagggaa tactacccta atcgtttaac caaacgtttt aagaatattc
     2761 ctcttgagga agaagctcct gaaatcatta tgaccttgac acgccaactt ggatttaaag
     2821 acgattatga ccgtttttac actttattcg ttaaggaagt tcgtgatggt aaattaggtc
     2881 aatacacctt agatcaggtg ggagatatgg atgccgacta gtattaaagc tattaaagaa
     2941 agcttagagg ccgttactag cctcttagac cccctctttc aagaattggc aaccgacact
     3001 aggttaggcg tccaaaaagc tctaaaaagc cgacaaaagg ctattcaggc cgagttagca
     3061 gaagaagaac gattagaagc catgctttct tatgaaaaag ctctttataa aaaaggttat
     3121 caagccattg caggtattga tgaggtggga cgtggtccct tagcaggtcc cgttgtggca
     3181 gcttgtgtga ttttacctaa gtattgtaaa attaaaggcc ttaatgattc taaaaaaatc
     3241 cctaaagcta agcatgagac catttatcag gcagtgaaag aaaaggcttt ggctatcggt
     3301 atcggtatta ttgacaatca gctgattgat gaggtcaata tttatgaagc aaccaaacta
     3361 gctatgctag aagccattaa acagttggag ggccaactca cacaaccaga ttatctcttg
     3421 attgatgcca tgacattgga tattgctatt tcgcagcagt ctattcttaa aggcgatgcc
     3481 aattccttgt ctattgcagc agcatcaatt gtagctaagg tcaccagaga tcagatgatg
     3541 gctaactatg atcgcatttt tcctggttat ggcttcgcta aaaatgcagg ctatggcacc
     3601 aaagaacatt tacagggatt aaaagcttac ggcataacgc ctatccatcg taaaagtttt
     3661 gaacctgtta aatccatgtg ctgcgattca actaatcctt aaaaagttct gtttttagca
     3721 gaattttttt cttttttacg aataaatgaa gtaaggaggt aacttagtga atcattttga
     3781 actttataag ctaaagaaag ctggactgac taataaaaac attctcaata ttcttgacta
     3841 ccaaaaacaa caggaaaaat cgttgtcact tcgagatatg gccgttgttt ctggctgtaa
     3901 gcatccgtct cactttatag aagcctataa gcagttagat attcaaaaat taaaaatgga
     3961 atttaaacaa tttcctagta tttctatctt agataagcat tacccaatgg cattgaaaga
     4021 aatatacaat ccacctgtcc tcttgttttt tcagggaaat ttagaccttc ttgagaaacc
     4081 taaattagcc attgtcggct ccagacgctc aagcgatacc ggagtaaagt ctgtccgtaa
     4141 aattcttaaa gaactcggga accgttttgt gattattagc ggacttgctc gaggtatcga
     4201 cactagtgct catttagcct gccttaaaaa tgggggacaa accatcgcta ttattggaac
     4261 agggttggat cgcttttacc ctaaagaaaa tagggagttg caaactttct tagggaaaaa
     4321 tcatcttgtg ataacagaat acggtccagg agaagaagct ttatcttatc actttccaga
     4381 acggaatcgt attatcgcag gccttagccg aggtattctt gtcgttgaag caaaaaatcg
     4441 ttcaggttcc ttgattactt gccaaattgg gatagaagaa ggccgagaca tttttgctgt
     4501 cccaggaaac attttggacg ggaaatccga aggttgcctt cagttaatta aagagggagc
     4561 aacatgcgtc acgtcgggca tggatatcct ttcagagtac taaaaaagtt aaagtttctt
     4621 cctatatagc attttttatt gacagtttct taaaaatggg atattatgct ataagcttta
     4681 aacaaactgt tataggaagt gtgtcgaatt gggaacaaaa actacaacta agccaaaaac
     4741 gggtactaaa aagtccacca ctaaaaagaa atcaacagcg aaaaaaaatc tcgttattgt
     4801 tgaatcacct gccaaagcta agactattga aaaatatctt ggacgcagtt ataaagttgt
     4861 tgcttcggta ggacatattc gtgacttaaa aaaatcgtct atgtctattg attttgataa
     4921 taattatgag ccacaataca ttaatatccg tggtaaggga ccccttatca attctttaaa
     4981 aaaagaagcc aaagctgcta aaaaagttta tttagctagt gacccggatc gtgagggaga
     5041 ggctatttct tggcatttgt cacatatctt aggacttgat cctcaagata ataaccgagt
     5101 ggtttttaat gaaattacga aagatgcagt taaacatgcc tttgtggagc ctcgtcagat
     5161 tgatatggac ttggtagatt ctcaacaagc acgtcgtgtt ttggaccgta ttgtgggata
     5221 ttccatttca ccaattcttt ggaaaaaggt caaaaaagga ctttcagcag gacgtgtcca
     5281 gtcggttgct ttaaaactca tcattgaccg tgaaaatgac atcaaagcat ttgttccaaa
     5341 ggaatactgg tctattgatg gtctttttaa aaaaggaact aaaaagtttc aagcgacttt
     5401 ttacggtatt aatggcaaaa aaacaaagct tgataataac aacgatgtca aagaggtatt
     5461 agcaaagcta acaaatgaag tttttcttgt cagcaaggtt gacaagaaag agcgacgtcg
     5521 taatgctccc ttaccttaca caacatcatc tctacaacaa gatgctgcta ataaaatcaa
     5581 tttcagaacg cgtaaaacca tgatggtagc ccaacaactg tatgagggga ttcatttagg
     5641 agaaaacgga actcaaggtt taattaccta tatgcgtact gattctacac ggattagtcc
     5701 tgtagcgcaa aatgatgctg cccaatttat cagtaatcgt tttggagcta attattctaa
     5761 acatgggaat cgtgtcaaaa ataccagtgg tgcccaagat gctcacgagg ctattcgtcc
     5821 atctagcgtc aatcatacac cagaagctat tgccaaatac cttaacaagg atcagttaaa
     5881 actttatacg cttatttgga atcgttttgt ggctagccaa atgacagcag ctgtttttga
     5941 taccgtaaag gtgaacttgg agcaaaatgg cgtgattttt gttgccaatg gaagccaaat
     6001 gaaatttgac ggttatatgg ctgtttataa tgactcagat aaaaacaaaa tgttacctga
     6061 aatggaagaa ggagaaaacg ttaaaaaggt atcaacttct ccagaacagc acttcacgca
     6121 acctccggct cgctactccg aagcaactct gattaaaact ttagaagaaa atggggtcgg
     6181 tcgaccatca acttatgccc caaccttaga agtgattcaa cgcaggtact atgttaaatt
     6241 atctgctaag cgctttgagc caactgagct tggggaaatt gtcaacaagc ttattgtcga
     6301 atttttccca gatattgttg atgttgcctt tactgccgag atggaaggta agctagatca
     6361 agttgaaatc ggtgaagaag aatggcaaca tgtcatcgat caattctatc aaccctttgt
     6421 caaggaattg aataaggctg agtctgaaat tgaaaagatt caaatcaaag atgagccagc
     6481 tggttttgac tgtgatgttt gtggccaccc aatggtcatt aagttaggac gttttgggaa
     6541 attttacgcg tgtagcaact tcccagagtg ccgtaataca aaagccatca ctaaagaaat
     6601 tggtgttact tgtccggttt gtcataaagg acaagttatc gagcgtaaaa ccaaaaaaaa
     6661 taggatattt tatggttgtg atcagtatcc agattgtgag tttatctcgt gggatttacc
     6721 tattggacgt gcttgcccta agtcaggtga ctatcttatt gagaaaaaag ttcgtggtgg
     6781 caaacaagta atgtgtagca atgagacctg tgattacaag gaagaaaaaa ttaaataaaa
     6841 aaagaattgg aactccttgt cttgttccaa ttcttttttt atttacaaag gtatttttaa
     6901 ctacaaaaat cgctagttcg tttttaaaag gcttaaaaat tcctcaataa ctggactgaa
     6961 actcgcatca tctcggtaag caatcccaat agggtaaaaa ttatttttat cttgcaaagg
     7021 aatccaaact aagttatcta cctgattaag actctcaaaa tcactcggga ggatagtcaa
     7081 ggtatttaaa tcgtgcaaac tatgaataag aacttcccaa tcattgtgtt taaagacaat
     7141 atctggttca aaaccgagag ctcggcattt tctaggaagc atttcgccta acatgtagtg
     7201 atcgtttaaa gaagcaatct tatagccttt taaatcttta agttttaact gaggatgttg
     7261 cgccaaaggg tgctgtttga ggagaacgat actgacctta tagccttttg tggacgtttg
     7321 taatagctca atggtaatat ccttttgaat acttagaaag gagaggagac caatatcgat
     7381 ttttccttgg gacaataatt cttataattt gcgagaacca tcctgtataa gtgataattc
     7441 aacatttgag tgagtagcca taaagattga aatttgtttc atgaattgta tagcaaacag
     7501 tgatgttaat tcaacacgaa tcatctcttt ttttcttgat ttaattgctg aatatccgca
     7561 acaatttgat taaagtcatc cacaaggtct ttgcctttgt gataaagaat atctccagct
     7621 tcggttagta gtagctgtca atcactttgt gtaaaaagcg tcgtgttcaa actaggactc
     7681 tagcctttta attgattgag aaagagtatg ttgcgttaca aacaaacttt tagcagcgtg
     7741 ggaatgattt tttgtttcag caactgcaat aaaataggtt aattatctaa tatccatagt
     7801 agatccttca tgattggtca gggaattgcg tctctacttg gtttacaacc tattttgatg
     7861 tctctcctta ttgccatgat tttctgcttc cttattgttt ctccaattac aacggtagga
     7921 attgctcttg cgattaactt atctggtatt ggttcaggtg ctgcaaactt aggtatttgt
     7981 gccgcaagct ttggactttg cttagcaggt tgggctgtta attcaaaagg aacttcactt
     8041 gctcacgtct tgggatcacc gaaaatttca atggccaacg ttttatcaaa accaaaaatc
     8101 atgttgccaa tgttatgttc tgcagctgta cttggtgtta ttggtgctat ctttaatatt
     8161 caaggaacac ctgctagtgc aggctttggg attagttgtt tgatcggacc tatcaatgcc
     8221 cttaatttag ctaaaggcgg atggtctcta gtaaatatcc tacttatcat tatcattttt
     8281 gtaagggcac caattgttct caatatgatc tttaactacc tttttatcaa ggtgcttaaa
     8341 gtgattgatc caatggatta caaacttgat atctaagaaa ggaacttttg atgaaattaa
     8401 aattatataa tgttagaggc gaagaagcag tcttagcaaa aaaatgggct gatgacaatg
     8461 ggattgagat ttccttgaca gagtcttcct taactcctga aacggttaaa gaagctgaag
     8521 gctttgacgg gattgccaat gcccaaattg gtcctttaga tgatgcgatt tatcctctct
     8581 taaaggaaat ggggattaaa caaatcgctc agcgcagtgc tggtgtcgat atgtataacc
     8641 ttgatttggc caccgaaaat gacattatta tcacaaatgt tcctagctat tctccagaat
     8701 ccattgcaga atttacggtt actattgccc ttaatttaat tcgtcatgtg gagttgattc
     8761 gtgaaaatgt taaaaaacaa aatttcactt ggagactccc tatccgcggc cgtgttttag
     8821 gtgatatgac agttgcgatt atcggaactg gccgtattgg tctagctact gctaaaatct
     8881 ttaaaggttt tggctgcaag gtcgttggat atgatattta ccaaagcgat gctgccaaag
     8941 ctgtcttgga ctataaggaa tctgtagaag aagcgattaa agatgctgat cttgtttcct
     9001 tacacatgcc accaactgca gagaacacac accttttcaa tgctgattta tttaaatcat
     9061 tcaaaaaagg ggctattttg atgaatatgg cgcgtgctgc tctcattgag acgcaagatt
     9121 tactggatgc tttggatgca ggcttactga gcggagctgg tattgacact tacgaatttg
     9181 aaggacctta tatacctaag aattttgaag gtcaagaaat tacagattca ctatttaaag
     9241 ctttgattaa ccatcctaag gttatttata cgcctcatgc agcttattgt actgatgaag
     9301 ccgttaaaaa cctagttgaa ggtgccctta atgctacagt agagattatc aaaactggga
     9361 caacgacaac tcgtgttaac taacaactga tttgacgtcc attataaagg aataatttta
     9421 aaaattcctt tataatggac ttttttgcat tagtccgtta taatagactg taagaaaggg
     9481 gactcttatg acatatatag cgcttattgg agatattatc cagtccaaac aattgacgga
     9541 tcgttccaaa gttcaaaaaa ctttagcagc ttatttagac gacctcaaca aaacctttgc
     9601 tccttatatt atttctaaat tatccctaac gttgggagat gaatttcaag gactttttca
     9661 ggtggatact cctattttcc atctgattga tttgattaat catcacatgg atatccctat
     9721 tcgatttgga gtaggagtag gttctattct aacagacatc aatcctgata ttagtattgg
     9781 ggctgatgga cctgcttatt ggcatgctcg tgaggctatt cgttatattc atcaaaaaaa
     9841 tgattatggt aatactacat tagctcttcg gactggtcat cacaaccagg atgatgtatt
     9901 aaacagttta ctagcagctg gagatgctat taaagccaac tggcgagcta gtcaatggga
     9961 aatttttgac acgcttttag atttgggcat ttatgaggaa tactttgatc aacaacgact
    10021 aggaaagcaa ttatctctta gctctagcgc tttgtcaaaa cgtctaaaaa gtagtcatgt
    10081 taaaatttat ctgagaacac ggcagagtgc gctaaactgt ctcaaacaaa tcaaggagga
    10141 aactgatgat taatggtgtc tctcactacc tagctcaaac ccctacactt accctgtttc
    10201 tcatttgtca tgtgttatca gactaccagt tacaaagtca gcaagtagct gatttaaaag
    10261 aaaaacattt gacttattta ggttaccact tgattggagt tagcattccc ctaatttgtt
    10321 taaccctcat tatcccacaa gcatggttga tgagcctatt ggttatgata agtcacgcgc
    10381 ttattgactg gctaaagcca aagatggcta actctctaaa atggaaaaga gagtggattt
    10441 ttctgctgga tcaatgctta catatcgcta ttagttcatt tgctgggcta aggttagcag
    10501 gggtgacctt gcctaattgg cttccgatat ctattttgat gaccgtttta tttatcctac
    10561 tcattaccaa accaactaat attgtcttta aactcttttt cagcaaatac cagcctgacc
    10621 aaggagaaaa aatggacacg atcataggag cgggggctac cataggaatt ctagagagaa
    10681 ttgttattgg ggtgtgtatg atcatggggc aatttgcttc aataggtctg gtctttaccg
    10741 ctaaatctat tgctcgctat aataaaattt cggaaagtcc agcttttgca gaatactacc
    10801 taattggttc tttatttagt attctcagtg tttttatcgc agcatggatt tgtttcttct
    10861 agttgaaaag ggttcccgta gaaagaggct aacgcttagt ctctttcttt ttctttcttc
    10921 ttatttctgt tataataagt caggtaacta cattagaaag atttgtgggt ctgtcatcta
    10981 agagatgtca ttcccgaaga ggtattagtg acttgtctca atcaactgca acttatatta
    11041 atgttattgg agctggacta gctggttctg aagctgccta tcagattgct aagcgcggta
    11101 tccccgttaa attgtatgaa atgcgtggtg tcaaagcaac accgcaacat aaaaccacta
    11161 attttgccga attggtctgt tccaactcat ttcgtggtga tagcttaacc aatgcagtcg
    11221 gtcttctcaa agaagaaatg cgtcgcttgg attccattat tatgcgtaat ggtgaagcta
    11281 atcgtgtacc tgctggagga gcaatggctg ttgaccgtga ggggtatgca aagagtgtca
    11341 ctgcagagtt ggaaaatcat cctctcattg aggtcattcg tgatgaaatc acagaaattc
    11401 ctaacgatgc cattacggtt atcgcgacgg gaccgctgac ttcggatgcc ctggcagaaa
    11461 aaattcacgc ggtaaatggt ggcgatggat tctatttcta cgacgcagca gcgcctatca
    11521 ttgataaatc tactattgat atgagcaagg tttacctcaa atctcgctac gataaaggcg
    11581 aagctgctta cctcaactgc cctatgacca aggaagaatt catggctttc catgaagctc
    11641 tgacaaccgc agaagaagcc ccgctgaatg cttttgaaaa agaaaagtat tttgaaggct
    11701 gtatgccgat cgaagttatg gccaaacgag gcatcaaaac catgctctat ggccccatga
    11761 agccagtcgg cttggaatat ccagatgact atacaggtcc tcgcgatgga gaatttaaaa
    11821 cgccatatgc cgtcgtgcaa ttgcgtcaag ataatgcagc tggaagcctt tataatatcg
    11881 ttggtttcca aacccatctc aaatggggtg agcaaaaacg cgttttccaa atgattccag
    11941 ggcttgaaaa tgctgagttt gtccgctacg gcgtcatgca tcgcaattcc tatatggatt
    12001 caccaaatct tttaacggaa accttccaat ctcggagcaa tccaaacctt ttctttgcag
    12061 gtcagatgac tggagttgaa ggttatgtcg aatcagctgc ttcaggttta gtagcaggaa
    12121 tcaatgctgc tcgtttgttc aaaagagaag aagcacttat ttttcctcag acaacagcta
    12181 ttgggagttt gcctcattat gtgactcatg ccgacagtaa gcatttccaa ccaatgaacg
    12241 tcaactttgg catcatcaaa gagttagaag gcccacgcat tcgtgacaaa aaagaacgtt
    12301 atgaagctat tgctagtcgt gctttggcag atttagacac ctgcttagcg tcgctttaac
    12361 actaaaaaag agttcctttt cagttgagtt gctgaagagg acttttttac ttgctgattt
    12421 gagcgctagc tatgcaaaag aatgcacaaa aaatacatga atagactatg gtcactagaa
    12481 gcaatattat ataatgaata gtactcatta gttgattcag tttggaggtt ttagcatgca
    12541 gcagcaagtc gcaataacag aaacagtttt acgggacggt catcaaagtt tgatggcaac
    12601 gcgtttatct attgaggata tgcttcctgt ccttaccatc ttagacaaga ttggttacta
    12661 ttccctagag tgttggggag gagcaacctt tgatgcctgt attcgttttt tgaacgaaga
    12721 cccttgggaa aggctgcgca cgttaaaaaa aggcttacct aatacccgtt tacaaatgtt
    12781 attaagaggc caaaaccttt taggctaccg tcattatgca gatgacatcg ttgacaaatt
    12841 cattagttta tcagctcaaa atgggattga tgttttccgc atttttgatg ccctaaatga
    12901 cccacgaaat atccagcaag ccttaagagc tgtgaaaaag acgggtaaag aggctcaact
    12961 gtgtattgcc tatacaacta gcccagtgca cacgcttaac tattaccttt ctttagtgaa
    13021 agaattggtt gagatgggag ctgattctat ctgcatcaaa gatatggctg gtattctaac
    13081 tcccaaagcg gctaaagagt tagtatctgg tatcaaagcc atgactaact tacctcttat
    13141 tgtgcatacc catgcaacta gtggcatttc tcagatgacc tatctagcaa cagtagaagc
    13201 tggtgctgat agaattgata ctgctttgtc accttttagc gaaggtactt cacaacctgc
    13261 tacagaatcc atgtatttag ccttaaaaga agcaggttat gatatcacat tagatgaaac
    13321 gctactggaa caggctgcta atcaccttcg acaagcccgt caaaagtact tagcagatgg
    13381 cattctggat cccagtcttc tctttccaga cccaaggaca ctacaatacc aagttcctgg
    13441 aggcatgtta tctaatatgc tatctcagct gaaacaagcc aatgccgaat caaaattaga
    13501 agaagtttta gcagaagttc ctcgggtaag aaaagacttg ggttatcctc ccttagtaac
    13561 accgcttagc caaatggtcg ggactcaagc tgctatgaat gtgattttag gaaaacctta
    13621 ccagatggtg tctaaggaaa ttaaacaata cttggcaggc gactatggca aaacaccagc
    13681 accagtcaac gaagacttaa aacgaagtca aattggctca gctcctgtca ccacaaatcg
    13741 tcctgcagat caactaagtc ctgaattcga agtgttaaaa gcagaagtag cagatttagc
    13801 ccaaacggac gaagatgtgc tgacttatgc cctcttccca agcgtagcaa aaccgttttt
    13861 aacaaccaaa taccaaactg atgacgtcat taaagtgact gcctttatca aagcataata
    13921 ggctatctta atcagataga aacaaaaata tggagatcac atgacctatt cctttattga
    13981 tatcttacaa gttaccttag tatccttgtt cttagtgtta acctgtttaa cagggattat
    14041 gggattaatg atgctaagtg gtaaattatt gtcacaagaa aaaaaaactc tctctcaaca
    14101 agcttccagc attcctcaag agcaagaagc tgacaagttg agtggagaag ctctcgaaac
    14161 tcttttagcc aaagacgaat tggctagaat tgctgttatg actagtcttg cttatgctag
    14221 ccaagaagag tcaggaaaac ggtttgaagt tattgccgtg gaaaggaaat cctaatgaaa
    14281 agatatgaag tgactgtcaa tggacaggtt tatgaagtga gtttacgtga actagctgat
    14341 ggagaaactg ttgaagttag ccggccagca gcacctgcta ctgagaaaga aatgaatgcc
    14401 aacgctgctg gcggtggcat tcaggtaaag gctccaatgt ctggaacagt cttgtctatc
    14461 tttgctacag aaggaaaagc tgttaaaaaa ggagaagctg ttctggtctt agaggccatg
    14521 aaaatggaga atgaaattct agcacctgca gatggtctgg tttcaaaaat tcatgttgtg
    14581 gctaaccaaa cggtcgagtc tgaacaagtt ttgatttcat tctaggagga aaccatgcta
    14641 gacgtactca atcaaatggt tcaatcgtca ggattggctc atctaactgt taacaatctt
    14701 atcatgattt gcttggcctg tttcttttta taccttggta tcaaaaagga atatgagcct
    14761 taccttatgg taccaatcgc ctttgggatt ttactcgtta atttgcctat ggcgggattg
    14821 atggaccacc ctgccaatgg taatcctggt ggccttcttt attacctata taagggaact
    14881 agcctaggga tttacccacc tttaattttt ctttgtttgg gggcatcaac tgactttggt
    14941 cccttgattg ccaatcctaa aaccatttta ctaggaggcg cagcacaagt tgggattttc
    15001 ttagcctttt tccttgctat tatgttaggc atgacacctc aagaagcggc ttctgtcggg
    15061 attattggtg gtgccgatgg accaacagct atttatgtga cgacaaagtt ggcgcctgat
    15121 ttactctcta ctatcgccct agcagcttat tcttacatgg ctctggtacc tattattcaa
    15181 cctcccatca ttaaactctt gactaccaag gcggaacgcc aagtgaaaat gactcaagca
    15241 agaactgttt cacaaaaaga aaaaatcatt ttcccgatta tggtaacaat ctttgtcagc
    15301 cttttggttc caagtgctac aaccttggtt ggctgtttga tgctgggaaa tctggtcaga
    15361 gaaattaaaa ttgttcctaa aattgtagag aatctccaac aagtagttat gttttgcatt
    15421 acgatcattt taggattaac cgttggtgct aaggccaatg gagatctctt tttatcagcc
    15481 acaaccttga aaattattgc ccttgggcta atcgcctttg ctgctgggac agcagggggt
    15541 gtcttgatgg ggaaaatcat gtactattta tctggtaaca aagtgaaccc tatgatagga
    15601 gcagcaggtg tttcagctgt tccgatggca gcacgagtgg tccaaaagat cggacaagaa
    15661 gaagatccaa gtaatttctt actcatgcac gccatggggc ccaatgtagc tggcgttatt
    15721 ggctcagcca ttgcctcagg tgccttgctg gccttctttg gctaattgtg aaaaaggatt
    15781 agtaaattaa tcttaataaa tcattatcag gagttggaag caatctagct cctttttagt
    15841 agaaagctcg agtgataaac tattccaaaa taattataaa aaaagactgt cctgattgta
    15901 cggactatat caattgactt ttatagtcta gctgaaatag cagtgcaaaa ctaagacact
    15961 cgttttaaag tagtttttct aaaaaagcaa aataaaaggt cagagccaat aaatctgccg
    16021 cgcccccagg gcttaaatgc tgattaatga ggcattgatt gtaggaggac aaagccaatc
    16081 ttaggtcagt tgttgataaa tcttgttgca ggagtagtcg catatctgcc ttaacgcctt
    16141 tccatgcttc aatccctcca cgatgaatca gatttccatc ttcaacaata gacatcaaat
    16201 aaacaagaag acgaagctga ctggtttcgg ggtcattttg acttatcatc tgacgaaagt
    16261 agggcaaggc atggtctgtt aaggtcgtga atccctcgct tgcctcaccc cgaggtcctt
    16321 tgatgccata agttacaaag agttgctccc cataagtgaa ctctttttta gtgttcaaat
    16381 gaccaaggtc tgtttgaatc aaatgtgccg tcataggttt tactaaacga cagatagcta
    16441 gagtatcttc cttgctaaaa cgccctaaat cagtcattaa gtgaggtgtt cttgctagat
    16501 aggccccagt tgcaccaaga agtaaagcca tggaaaaatt caagccttta tgagtgttaa
    16561 ttccctgagt agcacaaaac atggtttcct cggccttttg acctagttgt ctaagacggt
    16621 taaacagaag aagtggctct tcttttgcat aagcaaaacc tgtttcgata taagcctgaa
    16681 aaaaaggaga gagtgctatc atactgtcga taaaggtcat aaaggacatg tcatcatggg
    16741 caccgttatc aaatcgatca actaaaccag gttttggaga tagtgagact tcataaagca
    16801 aagcttttaa ggccagttga ctaatacttg ttagaactgc cttagtcatt ttgctcaggc
    16861 ctcatttctt ttaagataaa ttcaagtgag ctattcaagt gttcatgtgt aatcaacgta
    16921 atttgctcat gatctttgtt cttcatacct cggtaaatca tccagtgttc agatagagct
    16981 cgtttacggc gttcaactga agaaatcgaa atttttctaa agtagaccaa ataagcttgc
    17041 aattctgtca caatttcttt taagcggacc atttggctat agctgtaaat caaattgtta
    17101 aaggcattaa aattatccaa aatacgattg gtgtcatcct ctgcgataaa ggtttcacag
    17161 tcttccaaca agttatgcat gactttaaaa tcttcttctg tcattaaatg catcgcttgc
    17221 accgttgcga gagtctctag agcttttcga atttcaaaaa tttcacaggc atccttgata
    17281 ctaactcctt tgacaataat gcctttttta ggaatgtgct ctacaaggtg ttcttcagac
    17341 aataatccca aagcgtagcg aataggtgtc ctgctaatat taagcgcaat ggaaaactct
    17401 ttttcattaa tgcggcttcc aacaggaatt tgtcgcaaaa taatggtctt tttaagagcg
    17461 ttataaaagg ctattttcaa agggatattt cttgataggt caagattttg tttaacggct
    17521 tcgataatag gattcatgtc atctccttaa tgctagtgtc ctcactttat tatacttaaa
    17581 tttgtgaaac attccaatag gactagatga gttctgaaaa attaaagcca tgctcacaaa
    17641 aggacatgac tttaatcatc tcataaggaa taaggtagta gtgttatcgt tggtttattg
    17701 tggaatgtaa agtggcattt gtcccatggc aataatggtt accacaaaga taacaaagat
    17761 gataagggca tatttagcag cctctttttg ccattccccc atgtcaagac ctgtcaagcg
    17821 aagaagaaga taaatgaagg caactaatgg actaagtaag tggaaggctt gtcccatgag
    17881 ggaagcaagt gccatagcca tgttactgaa accatattca gcaccagctt ctgcaagaac
    17941 aggcaagata ccatagtaaa aaccatcatt ggagataaag aaggtaccag gtgcagaaac
    18001 taaggcaata acgagccccc agaaacctgc cagttgtttt ggaataatgg ttgcaaaact
    18061 ttgtgcaaga gcgctagcca taccagaacc ttggaaaaga cccataaaga taccagcagc
    18121 aaagacgaga ataaccactt gaaccgcatc accagcatta tcaccaatgc gtttcgattg
    18181 atctttaagc actgggtaat tgaccatcaa ggcgatacaa gttcccaccg caaaaagaag
    18241 aagcggtgcc atagcaatag atttgttgaa agagccagca actaaccaag tgattaatcc
    18301 aatggttaag atagcattga aaacaaagtt tttaggacga cgaataaaaa gggtatctgg
    18361 gtcagtaatg tctgtgagtt gacgcatgtc ttcttcagac aagtcaataa caccaagtct
    18421 tgcacgttct ttacgcccca tgcttggagc aacccaacaa atcacataaa gaagagataa
    18481 aatcataccc ggtgcaagat agccaagaat ttcaggaccg acaccaagaa cagacatcgc
    18541 acgagcagta ggaccacccc atggcagtaa gttcataata gtattttgaa gaataatcaa
    18601 gacacctagg ttcatgattt tcatgtccaa ttttttatag ataggtaaga aagcagagca
    18661 acaaattaaa gtagtggttg ttccatcacc attaagagat actgcggcag caacgacagc
    18721 tgttgcgata agcaccttca ttggatcacc tttagcaaag cgaatcatct tttcagtaat
    18781 cggatcaaac aatcctgcat ctaacatgac agagaagaac aaaatggcaa atagcaacat
    18841 gatacctgtt ttagcagtat tgttgatacc atacataacc attggtccga tagctgttag
    18901 gtctttagca attccaccgt caccaacaaa ggcaacaaat ttggcatctg cattgaagtc
    18961 agctgaacca gtcagtataa cagcaattgt catgattaat ggaatcatga ccaaagcagt
    19021 aaaaggggtc atttttttct tcattaccac gtacataaag acaataatca tggcataagc
    19081 cagcattgtt aataacatca ttgttatctc ctttttcaag ttatgatttc agtatagatg
    19141 aaagcgctta taaaataaat acttctttgt gcagaaagac acaaaaaaaa caatttgaaa
    19201 attaattttg aaaaccattt ttattacatc agatgacttt aaattttcta taataatgat
    19261 gaacctagtt agatcaagct tttcttgcta tcagtatctt gatctcagct tttcaacagt
    19321 taccttttat tttgttttta ggtaactgtt gaaaagcttt atgaaacatt gaaagcgctc
    19381 ttagaaagaa cacaaaaaag ccatctcaaa aaagattggt agtatataat aaacttacta
    19441 gttccctaaa taggtaaatg tgaaaaagag gtagctcatg cagtttttac aaaaatggtt
    19501 taaacactct aaaaaagaga agttgaaaga atcctcgccc cttagtacag aaatagaacc
    19561 ttctgaaaac tgggaaaaga ttcccgctta tattcctgca gacaaaagtg attacaaaaa
    19621 ggttacttta attaccagtg ttattgctgc tggcgataga cctaatagtc aattcaaggt
    19681 taaacgcatt ctcaagcgca atcctgaggc aatcactgtt tctttaattg cttcaagtat
    19741 cgctgcaggt gtttacccag aaagtcagtt tcgggtgaca tccatctatt gcaaaaggta
    19801 gtcggtgaac gatactttag acgaactcat gacacaataa catataggag aaaaatgatg
    19861 ttacgaaaat ttaaaattac cattgacggt aaagaatatc ttgtagaaat ggaagaaatc
    19921 ggtgctcctg cccaagcagc tgcccccgct caaccaatat ccactccagt atctgtccct
    19981 actgaagcaa gcccacaagt ggaggaagcg caagcaccac agccagtagc tgcagcggga
    20041 gcggatgcca ttccatcacc aatgcctgga accatcttaa aagtcttagt agcagtggga
    20101 gaccaagtaa ctgaaaatca gccactattg attttagaag ccatgaagat ggaaaatgag
    20161 attgtggcct tatcagcagg aaccattaca gctatccacg tcggtccagg tcaagtggtc
    20221 aatcctggtg atggtctcat tacaattggt taatagtact ataactagaa taaatagaca
    20281 gaaaaggaag tgaatttgtg gacactttaa tcgcaggaat aacctccatc accattcctc
    20341 aaattgttat gatggtcatc ggtgccctct taatgtatct aggaattaaa aaagaatacg
    20401 aaccaacctt acttgtcccc atgggacttg gaaccattct cgttaacttc cctggttcag
    20461 gagttttaac ccaagtggtt aacggagtcg agcaagaagg ggtttttgag gctctcttca
    20521 attttgggat tggaacagaa cttttcccac tgttgatttt cattggtata ggagccatga
    20581 ttgactttgg tcccttgctt caaaacccat tcatgctctt gtttggtgct gcagctcaat
    20641 tcggaatttt ctttgttgtc gttgttgccg tactggctgg ctttgacatt aaagaagcag
    20701 cttcaatcgg cattatcgga gcagcagacg gaccgacttc tatctttgtt gccaaccaac
    20761 tggccaagga tttactgggt ccaattacgg ttgcagccta ttcttacatg gctctggttc
    20821 ctattatcca accatttgcc attaaattag tgacaacgaa aaaagagcgc cgtatccgta
    20881 tgacttacaa agctgagaat gtatctcaaa tgactaaaat cttattccca atcatcatta
    20941 ccttagtagc agggttcatc gccccaattt ctctaccttt agttggcttt ttgatgttcg
    21001 gtaacttatt acgtgagtgt ggtgtgctcg atcgcctgtc acaaactgct caaaatgaat
    21061 tggtaaacat tattagtatc ttgcttggat tgaccatctc tattaaaatg caggcagacc
    21121 tgttcttaaa tgtacagaca ctcttaatca tcgtctttgg tttacttgct tttatcatgg
    21181 attctatcgg tggggtcatg tttgccaaat ttctcaatct cttccgaaaa gagaagatta
    21241 atccaatgat tggcgctgcg ggtatttcag cttttccaat gtcaagtcgt gtcattcaaa
    21301 aaatggcaac agatgaagac ccgcaaaact ttatcctcat gtacgctgta ggtgccaatg
    21361 tctctggcca aatcgcctct gttatcgcag gtggtttgtt gctagcttac tttagctaag
    21421 aaaggaagga aaccttatga caattaatat ggaccatttg attagttcct ttgagctaat
    21481 ggcgcttggt atggcaggtg tctttatcgt gttaggcatt ctctatcttg tcgcagagat
    21541 cctgatcaaa cttttcccag ttagtaaata acaatatcat ttaagaaaat caaggaggta
    21601 aacgcaatgg atattaaaca aactgccgtt gctggttcac ttgaatcaag tgacctaatg
    21661 attacagtat cccctaatga cgagcaaacc attacaataa ccctagacag tagtgttgaa
    21721 aaacaatttg gcaatcacat tcgtcaactc attcatcaaa ccctagtgaa tttgaaggtg
    21781 acggctgcta aagtagaggc tgttgataaa ggcgctttag attgcaccat tcaagcgaga
    21841 accatcgcag ctgttcatcg cgcagctggt gttgaccaat acgattggaa ggagattgac
    21901 tcatggaacg tttaagaaga acaatgatgt ttgtgccagg tgccaatgca gccatgcttc
    21961 gagatgctcc tttatttggc gctgattcaa tcatgtttga cttggaagat tctgtttcac
    22021 tcaaagaaaa agacacctct agagctctcg ttcattttgc gcttaaaact tttgattatt
    22081 caagcgttga aacggttgtt cgtgtgaatg gtcttgattc ttgtggggtt ttagacatcg
    22141 aagctgttgt tttggcaggt gtcaacgtga ttcgtcttcc aaaaacagag actgcccaag
    22201 atattgttga tgtggaggct gttattgaac gtgtcgaacg cgaaaacggc attgaagttg
    22261 gtcgcacacg catgatggca gccattgaat cagccgaagg tgtcctaaat gctcgtgaga
    22321 ttgccaaagc ttctaagcgc ttgattggta ttgctcttgg ggcagaagac tatgtcacga
    22381 atatgaaaac gcgtcgttac ccagatggtc aagaattatt ctttgctcgt agcatgattt
    22441 tacatgctgc tcgtgctgct ggaattgctg ccattgatac tgtttattct gatgtcaata
    22501 atacagaagg attccaaaac gaagttcgta tgatcaaaca gttaggattt gacggtaaat
    22561 cggtcatcaa ccctcgtcaa attcctctgg tcaatgagat ttatacccca accgaaaaag
    22621 aaattgacca tgccaaacaa gtcatctggg caattcgtga agctgaaagc aaaggctctg
    22681 gcgttatttc cttaaatgga aaaatggttg ataaaccaat cgttgaacgt gcagaacgtg
    22741 tcattgccct agcaacagca gcaggtgttt tatctgagga ggacatttaa agatggtaac
    22801 aaataaactc ggtagagata ttcctcaacc atacgccgac caatacggtg tctttgaagg
    22861 agaactcgca aatatcaaac agtatgacga atcaagtcgc cgtattaaac cagttaagcc
    22921 tggagattct aaattactag gatctgttcg tgaagcgatt gaaaaaacag gtttaacaga
    22981 tggcatgacc atttctttcc accaccattt ccgtgaaggg gatttcatta tgaatatagt
    23041 cttggaggaa attgccaaaa tgggcattaa aaacctgtct attgccccaa gttccatcgc
    23101 taatgtacat gagcctttga ttgatcacat caaaaatggt gtggtcacta acatcacttc
    23161 atctggtctt cgtgacaaag taggggcagc catttcagaa ggactaatgg aaaatcctgt
    23221 ggttattcgc tcccatggtg gtcgcgctcg tgccattgct agtggggata tccatattga
    23281 tgttgccttt ctgggcgccc caagttcaga tgcttacgga aatgttaatg ggacaaaagg
    23341 gaaagcaacc tgtggttctt tgggctatgc catgattgat gccaaatatg cggaacaggt
    23401 ggttatcttg acagataatt tggttcctta tcctaatacc ccaaccaaca ttcctcaaac
    23461 agatgttgac tatgtggtaa cagtggatgc tatcggagat cctcaaggaa tcgccaaagg
    23521 ggcaactcgt tttacgaaaa atcctaaaga actcttaatt gcagaatacg ccgctaaagt
    23581 aatcaccaac tctccttatt ttaaagaagg attctctttc caaactggaa caggtggcgc
    23641 ttctttggca gtaacccgtt tcatgcgtga agctatgatt aaagaaaata tcaaagctag
    23701 ctttgctctt ggtggtatta ccaatgctat ggtggagttg ctcgaagaag gactggttga
    23761 aaaaattctt gatgtccaag actttgatca cccatcagct gtttctcttg gtaagcatgc
    23821 tgaacattat gaaattgatg ccaacatgta tgcctcacct ttgagcaagg gtgctgttat
    23881 caatcaatta gacacttgta ttttatcagc ccttgaagtt gatactaact tcaatgttaa
    23941 tgtgatgaca ggatctgacg gcgtgattcg tggcgcttct ggaggacact gcgacactgc
    24001 ctttgcggct aagatgagtt tggttatttc accacttatc cgcggacgca tcccaacttt
    24061 tgtagatgag gttaatacgg tcattacacc aggaacaagt gtcgatgtgg tcgtcacaga
    24121 agtgggaatt gccattaacc caaatcgtca agacttagta gaccatttca aatccctcaa
    24181 tgtgccacaa tttagtattg aggagctaaa agagaaggct tacgccatcg ttggcacacc
    24241 tgagcgtatt caatatggtg ataaggttgt tgctcttatt gaataccgtg atggtagcct
    24301 catggatgtg gtttacaatg tgtaaagaca cttatttttc aggcgaagct atccaactta
    24361 gtgatatgtt aagagcccgc gaagaaagag ctctgcgtca actgcattta ttaaaggaat
    24421 acccagaagg tagcttatta tcggtcacca tgaatatccc tggaccaatt aaaacctctc
    24481 ctaaacttct tgaagctttt gatatagtga ttaaggccat tcaaactgcc ttagctgacg
    24541 ataaaatttg ttaccagttg cgattactgc ctacaacggg ttatgagtat tacctcatca
    24601 caagtctacc tagccgcgac ctgaagttaa aaatgatagc cttagagaca gagttgccaa
    24661 taggtcgtct catggactta gatgtcttgg tcttgcaaaa tgatctgcct cattcaatta
    24721 gcagaaccgt attaggaggc tcccctaggc aatgttttat ctgttctaaa gaggccaaag
    24781 tctgcggtcg cctacgtaag cacagtgtcg aggagatgca gactgctatt tcaaaattac
    24841 tccattcatt tttcaataaa gacaaccaat catcgtcatc agataagaca ggttgatgag
    24901 gagaacaatt atgacgaaaa tccgtataac ggaaacagtt ttacgtgatg gtcaacaaag
    24961 tcagattgcc acacgtatga caaccaagga aatgattcct attctcgaaa cgctagacaa
    25021 tgctggttat catgcccttg aaatgtgggg aggagcaacc tttgattcct gcctacgctt
    25081 tttaaatgaa gacccttggg aacggctaag agccatccgt aaagcagtga aaaagactaa
    25141 acttcagatg ctcttacgcg gacaaaacct tcttggatat cgcaattatg ctgacgatgt
    25201 ggtcagatcc ttcgttcaaa aatccattga aaacgggatt gatattgtcc gtatcttcga
    25261 tgctttaaat gaccctcgca acttgcaaac agctgtttca gcgactaaaa aatttggagg
    25321 gcatgctcaa gttgccattt cttacacgac aagtccggta tataccattg attactttgt
    25381 tgaattggcg aaagcttacc aagctatcgg agcggactcc atttgtatca aagacatggc
    25441 tggtgtctta actcctgaaa taggttacca attggtcaaa tgtatcaagg agaatacaac
    25501 tatccctctt gaggttcata cccatgctac cagtggtatt tcagaaatga cttacttaaa
    25561 agtagcagaa gcaggagctg atattattga tacggcgatt tcctcttttt cagggggaac
    25621 cagtcagcct gccacagagt caatggcgat tgccttgacg gatttaggct ttgacactgg
    25681 cttggatatg caagaggtgg ccaaagttgc agaatatttc aacactattc gtgaccacta
    25741 tcgagagaca gggattttaa atcctaaagt caaagatact gagccaaaaa cattgattta
    25801 ccaagttcca ggtggcatgc tatcaaacct attgagccaa ttaaccgagc aaggcctagc
    25861 tgataaatac gaagaagtct tagcagaagt acctaaagta agggccgatc ttggatatcc
    25921 accacttgta acgccattat cacaaatggt tggcacacag gccttgatga atatcatctc
    25981 aggggaacgc tacaaggtag ttccaaatga aatcaaagac tatgttcgag ggctatacgg
    26041 tcaatcacca gcgccattag cagaaggtat caaagagaaa atcattggtg acgaagtagt
    26101 tattacttgc agaccagccg acctaatcga gcctcaaatg atttatctac gtgacgagat
    26161 tgctccatac gctcgttcag aagaagacgt gttaagctat gcgagtttcc cacagcaagc
    26221 cagagatttc ttgggacgcc gtgaagatcc tttctatgat gttccggttc aagaagttac
    26281 tgtacaactg gacattcaag actaacaggg aatgactaat cctcttaacc attaatctcc
    26341 ttggtgaaac gcatttgctg taagaacaat accaaaaacc tacctgattt tgataatcag
    26401 ataggttttt tatcttagta atgtttgaca gatgaagtgg cttgtatctt tttaataatg
    26461 ggttgggctt ctagcgaaca aaaataatct aaagacgttt tgggaaggag ttttgccaga
    26521 agttcccaat cattatcttt caatgcctgc ctagccgttg aggcactgat aggatcttgc
    26581 tgctgattga tagctttcct tggcaaaata tctaggagga taccagcttg ctgcaactgc
    26641 tctgccataa tttcattgta aatagcagtt actcggctgg ttggctcttc tccaacgtat
    26701 ctttttgtaa tctgtaaggt ttgagcaatc tttagaaaaa tagcggtatc cagcaaagct
    26761 tggctcttaa tcacgtcatt gtcttctttt tggaaatagg ctgggaaagt cgcttggcta
    26821 atcaaataag gacctgtttc atgatatatg acattatcaa gatgagctag accttcttga
    26881 atcaacctct ttctcaccga aaaaggaatt agactgcggt cttcactgac cataaacaga
    26941 tgaacccaat cattttctct tgcagctttc tctactaaaa attgatgccc taaggtaaag
    27001 gggttagcgt tgataacaat agcagcgact ttaccaggtg tttgctcagg cttattaagt
    27061 gtcatcagat aatcttgaaa accagttttt ttattttcca agaaactgat gtgattgtcg
    27121 atatggacaa tttcagtaaa accaagatct ttgaaaaaag cagcagaaga ggtcttcgta
    27181 taaacaaaaa gatgatagtt gccaagggca tattcttcat caatgagatg actcacaatc
    27241 ctattaagaa gtccttcacc ttgataggca ctggatacgc aaagacagcg caagctattt
    27301 ccaaagtaag aaccggtagc aatcacatca ttttgcgcat tcatgatggc acaagtataa
    27361 tctaaatgag tatccaaacg aataccttct tgatgaagca ggttctttac actagccatt
    27421 gtcgttttat ctgaaggaaa gacttttgaa atagtgtagt aaggcatttc ttctccttat
    27481 ctttaatttt attgaatcat aacataactt agtaccaaaa caggctgatc tatctaaagg
    27541 caactctgtt ttttatcaac gccatttttt aaaaggaaat cgaatgtgtt tgacagactc
    27601 aaaagtatca gcgatttttt gattgcgttc cacagtccgc aagttttctt ggtctaataa
    27661 gtcttgactt ttttccaaaa catcaacagt aatgtagtag agtttgatca ggcttcctat
    27721 cttatattta ccagattttg gagacatagc aaccacttca ttgagattac tatgaagcca
    27781 ttttttatga ggcttagcag gtatagtggc tactacaaag ccaagttgtt ccaaatgagc
    27841 ttgtgcctga tcaacagtaa gattaatcac atcatctatc gtgcgtagtt gcatctcttt
    27901 ttcatgtttt tgttccatac gtttttcgat aattggtcga ctgttatcaa tcgtttttcc
    27961 aataatttca gtagtgtcag gaacaattga catcagtttc ccaactactc ctagagcttt
    28021 tcttattctc tttggcatct tttatctcct caagtcgttt ctttttatta taacaaattg
    28081 tctaaaaata gtatgcgata aaccttgttc taaaagatta aa
//
LOCUS       DAUCWO010000016        27077 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUCWO010000016 DAUCWO010000000
VERSION     DAUCWO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27077
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.016"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            27..506
                     /locus_tag="VGY20_000653"
     CDS             27..506
                     /locus_tag="VGY20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ4273526.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            847..1470
                     /locus_tag="VGY20_000654"
     CDS             847..1470
                     /locus_tag="VGY20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ4273527.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKITTMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNASLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(1578..2480)
                     /locus_tag="VGY20_000655"
     CDS             complement(1578..2480)
                     /locus_tag="VGY20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ4273528.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            2654..4147
                     /gene="lysS"
                     /locus_tag="VGY20_000656"
     CDS             2654..4147
                     /gene="lysS"
                     /locus_tag="VGY20_000656"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ4273529.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            4283..4423
                     /locus_tag="VGY20_000657"
     CDS             4283..4423
                     /locus_tag="VGY20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273530.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLQS
                     KK"
     gene            complement(4526..4738)
                     /locus_tag="VGY20_000658"
     CDS             complement(4526..4738)
                     /locus_tag="VGY20_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ4273531.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(4948..6234)
                     /locus_tag="VGY20_000659"
     CDS             complement(4948..6234)
                     /locus_tag="VGY20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ4273532.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(6336..7262)
                     /locus_tag="VGY20_000660"
     CDS             complement(6336..7262)
                     /locus_tag="VGY20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ4273533.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            7466..7750
                     /locus_tag="VGY20_000661"
     CDS             7466..7750
                     /locus_tag="VGY20_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ4273534.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            complement(7909..8337)
                     /locus_tag="VGY20_000662"
     CDS             complement(7909..8337)
                     /locus_tag="VGY20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ4273535.1"
                     /translation="MNKSFKNLVIGAASGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQEEPVSDTLDKH"
     gene            complement(8330..8737)
                     /locus_tag="VGY20_000663"
     CDS             complement(8330..8737)
                     /locus_tag="VGY20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ4273536.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(8759..9538)
                     /locus_tag="VGY20_000664"
     CDS             complement(8759..9538)
                     /locus_tag="VGY20_000664"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ4273537.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESFWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(9535..10467)
                     /locus_tag="VGY20_000665"
     CDS             complement(9535..10467)
                     /locus_tag="VGY20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ4273538.1"
                     /translation="MTVTVKMLVQKVKLNVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(10832..11104)
                     /locus_tag="VGY20_000666"
     CDS             complement(10832..11104)
                     /locus_tag="VGY20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ4273539.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(11168..11605)
                     /locus_tag="VGY20_000667"
     CDS             complement(11168..11605)
                     /locus_tag="VGY20_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ4273540.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVSTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(11592..13724)
                     /locus_tag="VGY20_000668"
     CDS             complement(11592..13724)
                     /locus_tag="VGY20_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ4273541.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            13958..14113
                     /locus_tag="VGY20_000669"
     CDS             13958..14113
                     /locus_tag="VGY20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273542.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            14261..15163
                     /locus_tag="VGY20_000670"
     CDS             14261..15163
                     /locus_tag="VGY20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ4273543.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            15163..15978
                     /locus_tag="VGY20_000671"
     CDS             15163..15978
                     /locus_tag="VGY20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ4273544.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMILSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(16077..17501)
                     /locus_tag="VGY20_000672"
     CDS             complement(16077..17501)
                     /locus_tag="VGY20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4273545.1"
                     /translation="MGIFSKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(17520..19382)
                     /locus_tag="VGY20_000673"
     CDS             complement(17520..19382)
                     /locus_tag="VGY20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ4273546.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLTMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPEQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDTDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(19384..20226)
                     /locus_tag="VGY20_000674"
     CDS             complement(19384..20226)
                     /locus_tag="VGY20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ4273547.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            20554..21921
                     /locus_tag="VGY20_000675"
     CDS             20554..21921
                     /locus_tag="VGY20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4273548.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(21975..23525)
                     /gene="ftsY"
                     /locus_tag="VGY20_000676"
     CDS             complement(21975..23525)
                     /gene="ftsY"
                     /locus_tag="VGY20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ4273549.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEAVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNKFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(23525..24349)
                     /locus_tag="VGY20_000677"
     CDS             complement(23525..24349)
                     /locus_tag="VGY20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4273550.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAVREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDIIYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(24353..25150)
                     /locus_tag="VGY20_000678"
     CDS             complement(24353..25150)
                     /locus_tag="VGY20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4273551.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            25455..26582
                     /locus_tag="VGY20_000679"
                     /pseudo
     CDS             25455..26582
                     /locus_tag="VGY20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8018 a         5837 c         4829 g         8393 t
ORIGIN      
        1 tgtttagata aaggtaagga cttgatatgg ctaaaaaaac aaaactcaaa aaaaccttag
       61 tagaacaaat cctcgataag gctaatattg ctcatcaagg actcaagctt aatgctttag
      121 aaggcgactt tccagatgac cttcaaccat cagatattta caagacactt gctctgactg
      181 gtgatcaaac aggtcctcta attggcatta ttccattaac agaacactta tcagaaaaac
      241 aattggcaaa agtatcaggt aacaaaaaag tgtccatggt tcctcaaaaa gacttgcaaa
      301 agacaacagg ctatattcac ggtgccaata atcctgttgg gattcgtcaa aaacattcat
      361 atcctatttt tattgaccaa actgcactgg aaaaaggtca aataatcgtt tcagctggtg
      421 aagttgggcg ttctataaag atttctagcc aggctctagc tgatttcgtt ggcgcaagct
      481 ttgctgattt aaagaagaga aaatgaacac tactgccaac tttatctggt ttctttttta
      541 agtttttcgc cttgattaag ctagtctttg tttagtatta ttttgatctc aactgcttta
      601 acattgatcc tgttaatctt tttcttttgt agagacttga tatcacgaca aattcaggtc
      661 gatttgtctt agatctaata tgggctttac actttaatca gaacctagct attatacact
      721 tatactacgc ttttttactt tatcttttca ctataggtat tatttttgct tggtgatgcc
      781 taaacttttt ctggttactt ttttattttt agcgctcgca tcagttatct ctaaaaggag
      841 gcttaaatga aattatattt tgtaagacat gggaaaaccc tttggaattt agaaggtcgc
      901 tttcaaggcg ctgggggcga ttcgccatta cttgaagagg caaaagacga aattcactta
      961 cttggcaaag agttagctaa ggtggctttt gatgccgttt atactagcga ccttcaaaga
     1021 gctatggcaa cagcagctat tatcttagat gcttttgacc aacagccaaa actttaccac
     1081 accgaccagc ttcgggagtg gagattaggt aaactggaag gggctaaaat aacaacaatg
     1141 gcagctatat atccccaaca aatgctggct tttcgggaga atctagccca gttcaaacca
     1201 gatcagttcg aggctgaatc aatttaccaa acgactcaac gtgtctgcca tcttatccaa
     1261 tcgtttaaag ataaacatta tcaaaacgtt cttattgtag gacacggcgc taacttaaca
     1321 gcaactattc gctcattatt aggttttgaa cctgctcttt tactcgctaa aggtggctta
     1381 gataatgcca gtcttactat tcttgaaact aaagattatt taacttatga ctgtttaatt
     1441 tggaatgata aatcttttct agaggtgtaa aaccactaga caataataaa aaaagctaac
     1501 acattgattt cttactgaca agacactcac tttgctagct ttttttatta ttatctaatg
     1561 ggctattatg gtttcatcta tctggcatat tttaagcgca aaactttttc ttgctgcttg
     1621 gttaaccgaa gcaagataac cactttgtca atgctcatgt tactttcaag taaacgctct
     1681 gctgccatca tcaagccgtt gttgataccc atctgtaaaa ttggattttt cttgtcatta
     1741 atatcaaaaa taaatttatt atcaggtaaa tcaaagagta ctttaacggc accaaagagc
     1801 tgttcaaagt tatcaatagc cacatcataa acaggcttag tgcctggttt tagctgacga
     1861 cctctatgat taaaccacat ggaatgccaa ccaccattaa aggcacccat gatatcattg
     1921 tcataagagt ccccaacata tagtgtggtt tgtgggttca tgtcaaattg ttctgctgcc
     1981 aggttaaaaa tttctttttc tggtttttga aagcctgttg cttggctcac aatgacacgc
     2041 ttaggatcaa tataatcata aagccctaat ttacgaactt ttttcagctg atgttctgtt
     2101 ggcccattgg taataatccc cataggaaca ttctttgatt ttaaaaagtc aagggtcatg
     2161 cgcatctcat ctaacatggt aatgttctca agttcgtgct catagacttc ttgaaaatgt
     2221 actcccgcag cttcgtcgat ttctcgatag ccaaactcaa gcagtgtttc cttgcaacgc
     2281 cagaatcgga aatattctgt ggtccactcc cctgccatta ctcttgggaa tccaacatct
     2341 gaatagtgac gaaagcgaat ataagcttga ttcattactg acatatcaaa atctgggaag
     2401 cacttttcca tggcgattcg ataaggcgct tgctggtcgt aaattgtgtc atccacatca
     2461 aaaacaatag cagtaatcat gagtctcttt tcttttctag tttaatatcc caatcattat
     2521 actattttcc atttggcttt tcaattttat tttcaaaaaa gtgccccgct gcagtcgcat
     2581 tttccaacga aatataggtg tattctagcc tcttttatga tacaatggaa aggaaatcgt
     2641 attggaggaa aatatgtcta atcaacacat cgaagaatta aatgaccaac agattgtccg
     2701 tcgtgaaaag atgatggctc tggctgaaca aggaattgat cctttcggta aacgctttga
     2761 ccgcacagcc aattctgctg aattaaaaga aaagtatgct gataaaacaa aagaagaatt
     2821 acatgagtta aacgaaacag ctattgttgc tggccgcttg atgacaaaac gtggcaaagg
     2881 taaggttggt tttgctcatc ttcaagaccg tgaaggccaa atccaacttt atgttcgcaa
     2941 ggactctgtt ggcgaagaca actatgaaat cttcaaaaaa gccgatttag gcgactttat
     3001 cggcgttgaa ggcgaagtca tgcgtacaga catgggcgag ttgtcaatca aggcaaccaa
     3061 gttgactcac ctttctaaat cacttcgtcc tcttcctgaa aaattccacg gattaactga
     3121 tattgaaacc atttaccgta agcgtcacct tgatttaatt tcaaatcgtg aaagctttga
     3181 ccgcttcgtg acccgttcca aaatgatttc agaaatccgt cgttatttag acggtcttga
     3241 tttcctagaa gtagaaacgc ctgtgcttca taacgaggct ggtggtgctg ctgctcgtcc
     3301 tttcgttact caccataatg ctcaaaacat tgatatggtt cttcgtatcg caacagaact
     3361 tcatttgaaa cgtttgattg ttggtggtat ggaacgtgtc tatgaaattg gtcgtatctt
     3421 ccgtaacgag ggaatggatg ctactcataa tcctgagttc acttctattg aggtttacca
     3481 agcctatgca gactatcttg atatcatgaa cctgacagaa ggcattatcc aacatgctgc
     3541 taaagctgtt aaaggtgatg gccctattga ttatcaagga actgaaatcc gtatcaatga
     3601 acctttcaaa cgtgtccaca tggtagatgc tatcaaggaa gtgacaggtg ttgatttctg
     3661 gcctgaaatg actgtcgaag aagctattgc acttgccaaa gaaaaacagg tcccactcga
     3721 aaaacacttc acaagtgtcg gacatatcat caatgctttc ttcgaagaat tcgttgaaga
     3781 aaccttagtc caaccaacct ttgtgtttgg tcatcctgtt gaagtatcgc ctttggctaa
     3841 gaagaatcca gaagataccc gcttcaccga ccgcttcgaa cttttcatca tgaccaaaga
     3901 atacgccaat gctttcacag agttgaatga tcccattgac caattatctc gttttgaagc
     3961 acaagcacaa gccaaagaac ttggtgatga cgaagcaact ggcattgact acgacttcgt
     4021 tgaagctctt gaatatggta tgccgccaac cggtggactc ggaatcggga ttgatcgtct
     4081 ttgcatgtta ttaacaaaca caacaacaat cagagatgtg cttttattcc caacgatgaa
     4141 accttaactt ttttaagatt tgtattcata tcttaaaacc gataagtggc aatataacat
     4201 aacgttttat tatctctaac cagtcttcat taccatgaag actggtttat taacagtagc
     4261 ttatagaaag tgacttaaca agatgaccaa agaatatatc tcctacatta gtgctgctat
     4321 cttcttgctt tatggccttt tagctgccaa atggattttt cttgggctag gcttaatttt
     4381 ggtacttgtc ggccttgctg accgtcttca atctaaaaaa taacccttct ccgtgtcaac
     4441 tgcacccgat taccagcctt aaaatggcca agatgtcaag gtgcagttga ctagaagggt
     4501 tattttttac aaccacagag aggttttact tacgaaaagc gtctaagatg gttttaaatt
     4561 cttcattagt aagagtaatg cctttcccca ttttcgtatg gtctggactc cacgtgcgga
     4621 tgtcccattt cgcttctgcg ccattaaagc tgacgcggtt caattctttc gtccaacctt
     4681 tatcattttc tgataaggtg agtaaatgtt cctcaatgtt gaatgtaaat tctgccatta
     4741 gttttcctcc tcatctatac tattcgaaat taaagtggaa aaaatcatct ttttttcact
     4801 ttttttatga ctcattctcc tattattcta tcaagttttg agaaaataag ctattgtgat
     4861 atccaatttt caaaaaaaac aggctctctt ttcccaacta tggtcgtata ccatttggaa
     4921 aaagagggcc tctagtattt cttgttacta tgttctaaca gttttactgg tgccatcttt
     4981 ttgatagagg ttgacaagac cttccttgca agccctaatc atatcccctg gcttaacttc
     5041 tctcggtaaa gagatggtta agagttccat tggatttggg gcacggtcaa tcttttggcc
     5101 atccgcatca tgtaagtcct taacaaccgt ttcaaaatga cggaaacctg gtccataaca
     5161 ttcaatccga tcgccttcca tgatgacatt acgctgacga atggtcgctg tcatgctggc
     5221 tgagtcaaag gcaactactt ctccgacaaa tttatattgt ggaattttgc ggcgagcacc
     5281 aaataattgt tcattttcag ttgggatacc atagtaaaaa cctgtagcca actcgcgctg
     5341 ggcaaccttc cacaactcgt caatcaattc ctctttgata gcataaaaag cttctgggct
     5401 ttccatgtaa gcatctacag ccgccttgta acagttggtt acggttgaga cgtagtggat
     5461 agatttcatt cggccttcaa tttttaagct atcaacccca ttttcaatca ggtcaggaat
     5521 atggtcaatc atacacatgt caacagagga catagaatag tcttctggaa tttccccttt
     5581 taaggagcgg cgctctcctc caaatggcat gtcatacaaa tcatacttcc agcggcaaga
     5641 ctgtgagcag ccgcccctgt tggcatcacg gtgactcatg tggtttgaca aaacacagcg
     5701 gcctgaataa gagatacaca tggctccatg gacaaaggct tcaatttcca catctgtccg
     5761 cttacggatt tctgctaact cggccatatt aacctcacga gctaaaacaa ctcgggtcaa
     5821 acccatggct ttccaaaatt caaaggtctc gtaattggta gatgaagctt gcgttgacaa
     5881 atgaatttcc aaacctgggg cttctgttga acaaataaca atcaaggctg gatctgaaac
     5941 aatgaccgca tcaagcccca tatcacgcag ttgacgaaac cactcgcccg caccaatttc
     6001 gttcccttcg tgggtaacca tgttggcagc aacatagacc ttagctccac gcgcatgtgc
     6061 ataatcaatg ccttcttgca attcttccat agagaagtta ccagcgcggc ttcttaggcc
     6121 ataggcctgt cctccaacaa aaacagcatc tgcgccatag tcaatcgcaa cttttaattt
     6181 ttcaagtgtt ccagcaggtg ataagacctc gggacgtttt ttcatatgtg acatgtgata
     6241 ttctttctat tttcaagata ggtagattac ctgtgtaatg gtgtcataac cctgtcttgc
     6301 cctgaaatag taaactcact caagacaagg attacttatt taacggtttt tggatcgaac
     6361 tcataaaagc cagtatctaa tccacggcca gctggatgat gggcatgcac tgcttgatca
     6421 agcttttcag cttcttcttg ggtaaattgc ccagcttcta ataaggtttt agcctgaata
     6481 aacaatttag taatagcgac aaaatcatca cctggacagt aaatgccatc tagcttccaa
     6541 tgagttaagc catgcgcata cagctcaccc aatttagaca tcatgtcgat atcgttatta
     6601 atgaaaatat gagtgccatg attgtcttca taaatggagt aatggctgct agcatctcct
     6661 ggttctgcca agaaaagtcc tcgttcacga ctaacctcat catcaatctt tgtaaaatgg
     6721 taataatttt ctagcaaggg acgtttcgag tgatgaatca ccgaggcgcc gtaaactagc
     6781 acctctgctg gaaattccaa attttcagct aaggtgaata actctgctga tgggatttcc
     6841 cttgctaaaa cactttcaac agcaccatgt tgcccccaga aatttacctg acggctagaa
     6901 gtcacaaaaa cagaggtatc ataaatcagt ttaaagttat agccatccct tttattgaca
     6961 taaaacacac ctgcatcacc aaccacaaga taatcaacag caatctctat cattaagtct
     7021 aaaaagggtt tgatctgatc catcatatct tgatgcatca aagcattgca agctactgtc
     7081 aactctttgc ctgcatcatg cactagtttg gcaatttgtc tcaattcatc ataagaaaaa
     7141 ttatggggaa ggcgtaaccc gtaattggct tcaccaacat aaatacgatc aacaccagca
     7201 gctaataaag ctttgacttg ctcaatgctt tcagctgtgg ctgtaataat tattttttcc
     7261 atataagctt tattatacca aaaattttaa gaccgtaagc atctttcttg caaaagccca
     7321 agcattaagc tagtcccctt actgttatag catgataagt cacttgtcat tagtaaatct
     7381 tttcaaatct tttaaaaaaa gatgacaaga agagactttt atgatagaat tacttagtat
     7441 gttaaggaaa ggagaggaga ggactatgcc tgcaccgtta aagaagcata attcggttga
     7501 acaggatgat tcttaccaag aatctcaaag taaatttcga gattttcaag agttcaatgg
     7561 ccaaagtgca aagctaaaag aattagtctt ctttgctcgt atcgctagtt tcagccttat
     7621 taccgtttta tttgcatttg tcctgctcgt aatgaattta gcgccaatgt ttgctttctt
     7681 atttgcaagc atcattagct taggcattac ttccttattc gccaaggtta tcaaatcatt
     7741 actgatgtaa cttccagagt ccgttttacg tgactctttt tttgtgtcaa aaacaaaaaa
     7801 agattgaagc cacaataccc cccttcttca gttagcctaa aactaaccat cgggactagt
     7861 gatcagatcc ttcaatcttt tcttcttcta tgttttaggt cattttagtt agtgtttgtc
     7921 taatgtatca ctaacaggct cctcttgttc agtatagtca atgatgatgt cttcttcatt
     7981 taaatcaatg ataacatcat cttgcttttc tttcgcttca acttcggcaa gggtttcttt
     8041 tgttttttgc acgaaagctg ttgttttatc tttgagcaag tctaacatgt cctctttagt
     8101 taagtcacca gaagccaact tatctttcac atcatagaaa gtgtcacgtg ctaaatggct
     8161 gtattcacta cctttttctt tagcaaattg gtggtaatca tctggacttt ctttgtaggc
     8221 ttgataagct ttttcagcac gatttttcaa agccttgcct ttttctgttg ataagaagta
     8281 agctgccgct actcctgaag cagctccaat cactaagttt ttgaaacttt tattcatgct
     8341 tttctccttt ttttccaaat acttttgagg ctactttacc aactaatgct aattttccag
     8401 cttttgaaac attacctgta gcattagttg ctttttgccc aaaatgcctt gcttgactat
     8461 ttaaatctga aagactttct gacaaatcag caatagcaac aaacaatgga tcgatggttg
     8521 ctactttacc attaacatct tcaacaagga tattagcttt tgctaaaata tcattagttt
     8581 gatgaagtgt cacattaaca tcacttgtca atacagaaat cgtttttttg gcttcatcaa
     8641 ttgtttctga gactttcttt aatacaataa tcaagaaaat caccaaagca acaaatgcga
     8701 gtgcaataat catcagtgag attccaacta agtccataag tttctccttt ttctatcctt
     8761 attcttgata ataagaaatt cctttttgac gacgtctttt tataacaaag attaagccaa
     8821 taataattaa taaggcagac acatattggg agatgcgaat acctaaaaac ataagactat
     8881 ctgttcgcat accttcaatg actagccttc cactaccata ccaaattaag taaaatgcaa
     8941 agatttctcc atctaatagg ctttttggct tacgacgcca catcataata atgacaaatc
     9001 ccaaaagatt ccaaaatgat tcatagagaa aggtcggaat gcggtaactt ccctctataa
     9061 acatttgctt ttgaataaag cttggtaagt aatttaactg gctcacagct ttaccatagg
     9121 cttcttggtt gataaaattt ccccagcgac cgatagcttg agcgaccatg acacttggtg
     9181 cggcaatatc taaaaaatga atgggattga gcaccttgtt ataacagtaa gccaaaagta
     9241 caagagctcc tgtaatgaga ccgccataaa tagcaatacc gccattccaa atggctatga
     9301 tttcatctaa atgcttgaca taataagacc attcaaaaat aacatagtaa attcttgcac
     9361 caacaatagc aagtggaaaa gcaatcagaa taaaatcgaa aatagcgtca gatgatattc
     9421 cttttttggg tgcttctttt gatgctaagt aaaccgccaa aacaagtcct gacaagatac
     9481 atagcgcata ccagtggatg gctagtggac cacattttaa ggcaatggga ttgatcattg
     9541 actcacctca tttttggtga ttagttgagt taaacgatct tcaaaagttt ttgtagcatc
     9601 aaagcccatt tctttagccc gatgattcat cgcagcggct tcaataacca ctgaaacatt
     9661 tcgacctgtt tttaccggaa tacgaatacg agggattctc actcctgaga aagttatctc
     9721 ctcattaccg tttccgagac gatcaaaaac tttaccagcc tcaaagtttt caaggtaaat
     9781 ggccaattga acttgcgatg agtccttcac agcactagct ccataaagcg acatcacatc
     9841 aataatacct accccacgaa tttcaagcaa gtggcgcaag atctctgctg gctctcccca
     9901 aagggtttct tcatctttgg catagacatc cacacggtca tccgcaacca aacgatgccc
     9961 tcgcttaact aattctaaac cagtctcact tttaccaatt ccagaatccc cttggataag
    10021 aacccccata ccataaatat ccattaaaac gccgtgaaca ctagtacgtt cagctaaaga
    10081 agcgtcaaga aaataagaca tttctccagc taagcggcta gttgaaacac ggctacttaa
    10141 aagtgaaata ccttcttctt ttgcagcttg aaccatttct tttggaatgg ctaaatttct
    10201 agagacaacc actgcaggag tgtccttttt gaacatctct tttaaaacag aataacggtt
    10261 gtgagaagtc atctgtgtca gataagacca ctctttcatt ccaaatagct gcaacctttc
    10321 tggagcataa taatcaaaat aaccagtcat ttcaagacct ggacgtgaaa tatctgatgt
    10381 tgttatctct tttgacagca agttatctgt agcataaacc acattaagtt taactttttg
    10441 cactagcatc tttacagtaa cggtcataat ttctcctttt tcaatattat aacacaaatc
    10501 gaccgttaga cccttgcaaa tactcatttt ttcactacta aatgagaaca acgacaccaa
    10561 aaatggtctt agctctatta aaaactaggt tttatcattt agagatatga gtcaaaaata
    10621 agacaatggc aaacaaatac tttactagta tcattgagca attagtgctt attatggtgt
    10681 ttaaaaaggt atcatcttac taagtcaaaa agcaaaaagc cttatcatag ctgataagac
    10741 ccttcgctta cttacatatc aaaacatttt attatcatca gttaatcaca tggttgctct
    10801 tacgtgatga attatcattt taactgattg tttaccagaa ccagccatct tcttcttcat
    10861 ttaagacatc agcgtcttta cgacgacgag gacctcggcc ataacgttct tcaagcaagt
    10921 cttccttgta aggtaagaag atggccaaca aaatataaag caaaactcca aaaccagttc
    10981 cgtaaataag aagagccgct aagacacgcg ctagagcaag atcccaacca tacttatctg
    11041 ctaaaccagc aatgacacct gcaaccaagc gattttttcg ctgtttatag aatttagttt
    11101 ccacgtttct ttacctcctc attctatgat actagtttac attttttttc gcaaaataaa
    11161 accagtctta cgactgattt ttttccataa gtttcccgtg gcaatttcca caaacatatt
    11221 tagccagatt gatacgcctt tttcgttgat aaacctgccc acaggtttgg caactataat
    11281 gatgattagt ctttgattta gagctagttg agacataacg aagcccgttt acttgagcta
    11341 gcagatcttt gaaatcccta tctttatgat ggtaccctct tccttcaaaa taaagatggt
    11401 aatggcagag ctcatggcga acaattttcc gaaaaataag ctcaccgtgc tcctcaagca
    11461 tcctagggtt aaaatccaaa tgaccatctt ttggaaaaaa gcgaccgcca gttgttttta
    11521 ggcgcttgtt ccaataagcc ttgtggtgaa atggtttgcc aaaatcagcc agggaaactt
    11581 cttggacata attagtgagt gtcacgcggt ggcaataggc tgagattgac cttgtggcgg
    11641 tccaagtcaa tcttagagac ccaaacagtt accaagtcac ccactgaaac aacttgacta
    11701 gggtggttga caaaggtttt gctcatttca gaaatgtgaa taagcccatc ttcatgaaca
    11761 ccaatgtcta caaaagcacc aaagtcaacg acgtttctca cagttccttc aagcttctgg
    11821 ccaatttcca aatctttcaa atcaaggata tcttgacgta agattggtgc ttcaaagtca
    11881 tcacgaaggt cacgacccgg tttaaggaga tcagcaatga tatctttaag ggtttcctgc
    11941 ccaatagcca gtgtttctgc catttgggga acttgaactg ctgctaaagt tgccttggca
    12001 gcgtcgtcca agtcctgaat accaagtacc ttaaagagtt ctttgactgc tgggtaggac
    12061 tcggggtgaa ctcctgtgtt gtctaaaata tttttggctc ctggaattct gagaaaacct
    12121 gctgcttgct caaacgcctt agctcctaat cgaggcactt ttttgatgtc agcgcgtgat
    12181 gtcagtgctc cattttcttc gcggtatttg acaatatttt ctgaaatcgt tttgttcaaa
    12241 cctgacacat gtgctaataa agatggacta gccgtgttca cattaacacc gacttggtta
    12301 accacagtat ccacaacaaa accaagattc tcactcaact ttttctgact cacatcgtgc
    12361 tggtactgac cgacgccaat agatttagga tcaattttaa ccaattctgc gagtgggtct
    12421 tgcaagcgac gagcaataga aatagcagag cgcttttcca ctgtgaggtc tggaaactca
    12481 tgacgggcta actctgacgc tgaataaacc gaagcaccgc tttcgttaac gatgacatag
    12541 gacgtatttg ggaaatcttt caaaacgtct gctacaaagg cttcactttc ccgactggct
    12601 gttccgtttc caatggcaat aatatcaatc tggtaagtct caatgagctg agtcagtgtt
    12661 tctttagctg cttgaatctt cgtttggcta gctggtgcta cgggataaat aacctgagta
    12721 gtcaacaatt ttccagtctg atcaacgata gccaattttg cacctgttcg aaaggcaggg
    12781 tcaaacccaa gaaccatttt cccttttaaa ggagacacta acaaaagatg acggagattt
    12841 tcagaaaaga ggtggatagc cccatcttct gcggcatcac tgagttctga acgaacccgt
    12901 ctttccatag ctggaacaat tttctttttg atggtctgat tgatgacctc ttcaatataa
    12961 gggttggttt ctttgaaccg cacactgaaa aagcgttgca ttttctccaa gttatgctca
    13021 aaagacactt ttaagatgcc taacttttcg ccacggttaa gggctaaagt acgatatcct
    13081 tgcatgttag acacttggtc agaaaaatcg taatagattt ggaaaacctt tttctcatcc
    13141 aactgctcat ctttaagcgt tgatactaag cggctgtact gccaaatctc attgtaggtc
    13201 caagagcgta atttagcatc ttctgacatg gcctccacaa ggatgtccac agctcctgct
    13261 agagcttctt gtgggctggc aaatccttct gtgacaaagg gttctgctgc tgtttcaagg
    13321 ttttgcgcat tttgtaaaat caaacgtgct aatgggaaca agcctgcttc acgcgcaatc
    13381 gtcgcttttg tacggcgttt ttccttataa ggcaggtaca actcttccaa atcagcgagt
    13441 ttttcggttg cttcaatact cgttcgtaac tgatctgtca gttttccctg ttcctcaatt
    13501 ttagcgagaa tggttgcttt gcgctcattc agggtagtga gagacttatc catatcaata
    13561 atggacttaa tcaccacttc atctagattt cctgttactt ctttacggta acgtgcaata
    13621 aagggaatgg tattgccttg tgctgttagg gcaagcacct gttcaatttg atggagacta
    13681 actgacaaag cctctgcaat gttgtgatta ttgttatttt ccataatatt gctattataa
    13741 cacaaactat gttaatgcaa aaaatgatag cgaaacattg tcctaatata aagttgcgta
    13801 aagaatcaaa aaaagctact tttagtaaat aactgacgaa accctaaata acaataccta
    13861 attttaaaaa agaaagaagt aaacagcact tccgctttgg cattgttctc aaatgatagt
    13921 ataatagact tatcttgaat gaaagaaggt catagatatg gcaattaccc ataaaaaaaa
    13981 tgatgaactt gaaaaaatgc tagcaggctt tgcatcaatc cctagttttg ataagccttt
    14041 ggaagtcaat actgatggta aactagcgac caaagaaaaa acgacagaac aaacaaaacc
    14101 aagcaaacca tagctagttt gtttcattct cttaacatat cgtccattcg tttaaactga
    14161 gttgagatat ttttagcgat caggctaaca aaacgtatca gtcgaggctg aacattcgtg
    14221 tttcagcctc ctcttttatc atctagaaag gatatggtga atgtcactct tttccaacct
    14281 tcctcccagt gtcttgcaat ggtttgcgat ttttatgtcc attatcattg aagctcttcc
    14341 ttttgtgctc ctaggaacta ttttatctgg ttgtattgaa gtctttgtaa cgccagaatt
    14401 ggttcaaaaa tacctaccta agcaaaaatg cttgcgcatt ctgtttggaa cctttgttgg
    14461 atttgttttt ccttcttgtg aatgcgggat tattcccatc atcaaccgct ttttggaaaa
    14521 aaaggtgcct agttatacgg ccgtcccttt tctggcaaca gcgcctatta ttaatcctat
    14581 tgttttgttc gctacttatt ctgcttttgg gaattctttg cgctttctga tacttcgctt
    14641 ggtcggtgct gcactagtag ccattacact tggggtaatg ttagctttta ttgtagatga
    14701 taacattctc aaggataatg ctcaacccgt gcattttcat gattattctc atgaaagctt
    14761 gccaaaacgc atttatctag cactggtaca tgctattgat gaattcttcg acaccggtag
    14821 gtatctcgta tttggaacct tgattgcctc tgccatgcaa atatatgtcc caactcgtgt
    14881 gttgacaacg attgggcaca atcccttgac agccattttg atcatgatgc tgatggcttt
    14941 cattctatca ttatgtagtg aagccgatgc ctttatcgga gcttccttat tatccacctt
    15001 tggagtggct ccagttcttg cctttctttt aattggtcct atggtggata ttaaaaacct
    15061 tatgatgatg gtaaaggctt ttaaaggacg cttcattgtc caatttatcg gcgtatccgt
    15121 tcttatgatt gccgtttatt gtttacttgt gggggtgctc taatgattcg ctttttaatt
    15181 ttagctggct attttgaact aaccatgtat ctccagctgt ctggcaaact ggatcagtac
    15241 atcaatgttc ggtattctta ccttgcttat atttccatga ttttatcttt cattttagct
    15301 cttgttcaac tctatacctg gatgaaaaac attaaggtac atagccattt aaccggaaaa
    15361 atcgcaaggc tgaccagtcc ttttatcttg gtctttcctg ttttgattgg tctccttgtg
    15421 ccgaccgtga ccttagattc caccacggtt tctgctaaag gctatacttt cccattggct
    15481 gcaggagcct ctaaaacggg agtcagtgat gacggtacca ccattcaata cctcaaacct
    15541 gataccagcc tttactttac aaagtctgcc taccaaaagg agatgcgtca agaactccat
    15601 aaatataaag gcaagaaacc ggtcaccatt accactgaaa attatatgga agtgatggaa
    15661 ttgatctatc tctacccaga tgagtttctt gatcgtgaca tccaatacac tggctttgtt
    15721 tataacgaac cgggccatga caactaccaa ttccttttcc gcttcggcat tattcactgt
    15781 attgccgact caggagtgta cggacttttg accacaggta atcaaaccag ctaccctaac
    15841 aacacatggc taacagttaa aggtcgcctt catatggaat atgataaaaa cttagaacaa
    15901 catctacctg tccttcaact tgctgaggtt catcaaacca aagaacctaa taatccttat
    15961 gtttaccgcg ttttttaaag actaataaaa aagcacttgt tttttatcaa aacaaatgct
    16021 ttttgagttt tatcagttag cataggaaca gagtcaccat cttaaacaag acctatttag
    16081 gaaaaattat cctcaaggta acgtccattg gtttgaatga cttgtcggta ccaatcaaat
    16141 gattttttct tatagcgtgt gagttgacca gtaccatcat catttcggtc aacataaata
    16201 aaaccgtagc gcttacttaa ttgggctgtt gacatagaga cacaatcaat acagccccaa
    16261 gcagtgtaac ccatgacctc aacgccatct tcaatcgctt tggcaacttg tacgagatgc
    16321 tgactcatgt agtcaatgcg ataatcatcg tgaaccgtca tgctgccatc tgcggtttgc
    16381 accagctgat cctttgcacc taagccattt tcaacaataa acaaaggaat ctggtagcgg
    16441 tcatagtact gattcaagac caatcgcaga ccaattgggt ctatctgcca accccactct
    16501 gaagattcta aatatggatt ggacaaacca cctagaatat ttccctgacc agatgtgtaa
    16561 gcttcaggat tgtgggcttg tgtgacactc atgtaatagc taaaggatag aaaatcaacc
    16621 gtatgttccg ctaataattc cttgtctccg tcttcaaaaa caatctcaat cccattttct
    16681 ttaaaatacg acttaatata tgaaggatac ttgcctctca catgaatatc tgaaaataag
    16741 agattaagat tttcaaatgc atgcgcagca agtatatcac gaggatcaga ggtcattggg
    16801 taagcaggca tcgccaaaat catacaccca actttaacat caggattgat ttcatgcgct
    16861 agctttgtga cactagcaga tgctaccaat tcatgatgaa tagcttggta taaatcttgc
    16921 aggctcagct tttctttttc tgtcatgatg ccaccactgg taaagggcat gtgtaaaatc
    16981 gagttgactt cgttgaaggt caaccagtat ttgactttgt ccttatagcg ttccatgact
    17041 gtctgagcaa agcgctcaaa aaagccaatc agacgtctat ctgtccaacc attataggct
    17101 ttggccaaat gcagaggtgt ctcataatgc gacaaggtca ctaagggttc gataccatag
    17161 ttcaacaatt catcgaataa gtcatcataa aattgcaaac ctgcttcatt gggctggtca
    17221 tcgtcaccat ttggaaaaat acgagaccaa gcaatagacg ttcgaaagac tttaaagccc
    17281 atctcggcaa aaagagcaat gtcttctttg tagcggtggt aaaaatcaat cgcctctaaa
    17341 gttaaattat cgctggtagg actatctgtc cattctccta gcccaccatt tggtagaaca
    17401 tcttgtacgg acaaaccttt tgcgtctgcc tcaaatgctc cttccacttg gttggcagct
    17461 actgcgccgc cccataagaa atcttttgaa aaaattccca ttctccctct ccttttaggt
    17521 tataaaatga ctaacaaagc gtcctcttta gtcaccattt ttttgtcatc ttgaataata
    17581 atctccaact gctcagccga attggtcacg accacaggac tgatgagtga atagcctttt
    17641 gaagtaatga aatcagtatc catttttaga agtgcctggc ctcgtttcac gacatcgcca
    17701 acagacacta actgctcgaa accttgccct tttaactcaa ccgtatcaat accgacatga
    17761 atcaagactt ccacaccacg tgtgtttttt aaggcaacag catgcttggt tggaaagaaa
    17821 gtgatgactt caccatcaaa gggggcatag accacacctt cgcttggtaa tatggcaaat
    17881 ccttctccta ataacttgga agaaaaggtt tcatcaggaa cttctgatag aaaaagcatc
    17941 tcacctgtca ttggagcata caacgtttgt ttggttgaca agccgctctt aacttgtgac
    18001 atatctgctg caacactcac ttgctctggg gattcttcat caattcccat gaaccaagtc
    18061 aaactgaagg ctaacacaat cgtcgcagct ccggcaatca aagcatttgt aaaattagct
    18121 ccgcctgatg ggttgatgta ctgtggtaaa ccaataatag aaggtacgac aaaggtataa
    18181 gaagcaatat tgacaaaacc aataaaggct ccgaccagac cacctgaaat catggctgca
    18241 tagagtggct ttttaaattt cagtgtgacc ccataaagtg ccggttccgt gatacctgcc
    18301 agcaaagctg aaatccccgc ggcaagggca acttgacgtg tttgtttctg ctttgtttta
    18361 aaggcaacag ccaaagatgc ggctccttga gccaaattag aagccaacat tgctggtaaa
    18421 atcaagacat ctggtgttgc gaccgaagcg gccaaaaaga ttggtgcaaa agcccagtgc
    18481 atccccgtca tgacaacaag cggcataatc gctcccaaaa tggcaagtgc tagccacgga
    18541 gccacatgat aaatagcgag catggtatta gataaccctt gaccaataat cacaccaaga
    18601 ggacctacaa ccactaaagc caaaaagcca gaaatcaaaa tgattaaggt tggctgtaag
    18661 aagcttttca tcacgcttgg caccaagcga ttgacccatt tttcaatgta ttgcatcaag
    18721 taaaccatca tcaaaatggg aataacagat gagccatagc tagcaggagt gactggtgct
    18781 ccaaataaag ccaatggttt accttctgct accattgcaa caaaggcagg atgcaataaa
    18841 atccctgcga tcgtcgctgc taaaacaggt gtcaccttaa agcgcgccgc tgctgaatag
    18901 gcaatcatga caggcataaa ataataagcc gcatcgccaa agaagttaat aaaggcaacg
    18961 gtttgagagt ccgcactcgc taagccaagc atagtaagca agattcccac cactttgagc
    19021 attccaccac caagaagggc tggaatcagc ggtgtaatca cgccagcaat ggtttcaatc
    19081 aagcgctcaa ggatacttcc tttcgctttt gaagaatgat cttcatcttg attgtcaaaa
    19141 tgccctaggc ttaaaaaggc ttggtaataa ttattgacat cattgcccaa gataatttgg
    19201 tactggccgt ttttgcgcat cacgccaatc acatttgaaa tggctttgac ttgctgatct
    19261 ttaactttct catcattttt cagtactaag cgtagacgtg ttacacagtg tgtcacacgt
    19321 tgaatatttg ttttgccacc aaccgccgct aaaatagcct tggcagtttc ttgatacgtc
    19381 atattaagtc tcctttaaat gtttcactaa acgtttaaca tgaaccgtca aatacaaaag
    19441 ttcgcttgaa ttaaggtggt agttgtaccg cttttttatc tcctcactaa tatctaagac
    19501 gcaccggtat tctttagggt atttcgtttt caccactaaa agcagttccg catcatcatc
    19561 atcgtaagac atctcggata acacacgttg agcaaataat ttaatatggg tcataaaacg
    19621 gtaataatcg atggatgtgt catccaattc tgttttgaag tctgttttga ttttttgctc
    19681 aatataagaa acaatctccg tcaggcgatg tggctcctta aaaggagtgt ccaaactagc
    19741 attgacaaaa tgcatggcaa taaaagctgc ttcatcaata gctagagtga ctcctagatt
    19801 acgctcaatt agctctaagg cttttacccc tagactatat tcatcaggat aatagcgttg
    19861 aatttcccac cgtagtgggt tatgaatcaa cattccttgc tcatgccttt caatagcaga
    19921 gtgaatatga tccgttaaat taatatagag aatttcatct aaggttttgc ctaatttaat
    19981 tttacctaga ttgataattc tctctgagca agcaacaact tcttgtggca ccgtaataaa
    20041 taactctgtg aagcgattca tattgtcact attttttaaa acaaatgatg tttcgatagc
    20101 attaagctca atactatctc caactttttt tccaaaggca atcccttttc ccatcaataa
    20161 aatatctaat ccttgatggt tggtcgaaat cgccgcatta tggtttaaaa cgcgtttaat
    20221 caacataaaa gtcctagtcc tttagtatca caataaaaaa tgacctatcc aaaagctcgc
    20281 taaaaagcac ttttcgaata ggtccctgct cacaaaactg tgataacatc ctttaatatt
    20341 ctgattgtta tgtctctagc ctgcttacct gaagtaatca catctgagaa cgctttaatg
    20401 ttatcatctc tttaagattc tgtcaagcgt tttcaaaaaa ttctctgaaa gagcaactat
    20461 tgttattcgt atctattgat ttagcaaaaa agagtttctg acaattttaa gattatatga
    20521 tacaataaca atcgattaga taaaggagtt atcatgtcac atcatcaaca aaccgtttca
    20581 aaacaaacaa ttatggcgat tatcgccata gcactcattg gtttttcagg aattttgtct
    20641 gaaaccagta tgaatgtcac cttcccgaca ctgatgtcag tctatcagtt gcccttaaat
    20701 agcttgcaat ggatgacgac catttattta ctagcagtgg cgattatgat gaccacttcg
    20761 gctacactga aaaaaaatgt gcgggaaaga cccctctttt ttatggccac aggtcttttt
    20821 acatttggca ccatcttggc cgttctgacc cagtcctttg cgatcatgtt gctagcccgc
    20881 atttttcaag gcattggtac tggtctggta atgcctcaga tgtttaatat tattttagag
    20941 cgtgtcccaa tgcataaggt aggtctattt atgggatttg ctggtcttat tattagctta
    21001 gcacctgctt ttgggcctac ttatgggggc tttatgatta gccattttag ttggcaatgg
    21061 atttttatct gtatactccc tgtaccactg attgcaggta ttctagctta ttattacctc
    21121 gaagattctc cagtaagcga aaaagtaccc tttgactggt tggcatttat tgcactatcg
    21181 attagcttaa cttctgcctt attagctatt actagtctag aaaacggaag tgttaatttg
    21241 tattacttag ggctttttat tctcagcttt gtcctctttc tctacaagaa tctcacagct
    21301 aagcaaccct ttcttgatat tcgcattctc aaaattccct ctctaacctt tggcctgatt
    21361 cccttttttg tcttccagct gattaattta ggcataaatt ttctaacgcc aaactttatt
    21421 gtcatggaaa agattgctaa tagttctcaa gctggtatgg tgttactacc tggtacctta
    21481 ctcggagctc tactagcacc tgcttttggt aaactttatg atcaaaaagg agcaagactt
    21541 tcgctttatt taggaaatgc cttatttagt ttatcattga ttattatgac acttcaaaca
    21601 agacatttta tgcttttacc attcactctt ttatatattt tattcacgtt tgggcgtaac
    21661 atgggcttta ataatagctt agccacggct attcgagaat tgcctgccga gaaaaatgcc
    21721 gatgccacgg ccatttttca gatgatgcag caatttgctg gcgctctagg aactgctatg
    21781 gcatcactga tagcaaatag tcagccagaa ttcacaagcg gtgtccagtc tgtctacctt
    21841 ctctttacta tttttgctct acttgatttt atctttttct ttgctatgtt ttaccattta
    21901 gggaaaaaag gattagccta agctgactta cttaaaaacc agcctcgttc tgttgaacaa
    21961 gactggtttt tttcttataa aataccttct aaaagacctt tcataaagtc ttctgagtgg
    22021 aattctccaa tatcatccac tttttcacca aatccgatga acttgactgg aatgtctaat
    22081 tcttgacgaa tggctaggac caccccacct tttgcagtcc catcaatttt cgttaaaatt
    22141 aagcctgtta aaggtgttat ttttgaaaat tccttggctt ggctaagcgc attttgacct
    22201 gttgacgcat ctaaggccaa aagcgtttca tgaggggcat ctggtagaac gcgcttaatg
    22261 atacgaccca ttttttccag ctctgccatg agattttctt tgttctgcag gcgaccagcc
    22321 gtatcaatca gaagaatatc tacttctttt gccacagctt tttccatccc gtcaaaaaca
    22381 acagacgctg gatcagcctt ttcagggcca gtaatgactg gcacatctac tcggcgtccc
    22441 cattcgacta gctgagccac agcccctgct cggaaggtat ctgctgccac caacatgact
    22501 tttttacctt cttgtttgta acggtaagcc agcttcccga ttgaagtcgt tttaccaacc
    22561 ccgttaacac ctacaaaaag cataaccgtc aagccatctt gataattaat ggcttcatta
    22621 taacgcccgt ctttttcata gatatctacc aatttttcca caatcacacg tttcaaggca
    22681 tctggttttt tcgcattttc cagtttggct tcataacgca actcctctgt caaagttgtc
    22741 gccacatgta caccgacatc tgataagatc agcatttctt ccaagtcttc aaagaattct
    22801 tcatccactc gcctgaaatt ggcaaagaaa ctgttcagac gagctgagaa gccagtccgc
    22861 gtttttttca agctacgctt gtatttttct tgatctgtct caggaatggt atcgtaggtt
    22921 gccttgtttt gagaagcttc tgctgaggta gaggcctgat tttcttgtgg aacctcttct
    22981 ggcatcactg gaaccgttgc cgctgctttt tcttgaaggc ttttttcaag agcagcctta
    23041 cgacgatagt agtcttccat aacctgttgg ctactggatt gctcttttga agtgtctact
    23101 gcttcttcat tcgccgaatc atttgaggca tcagcttggg cttgttttgt tggaaactgt
    23161 tccacaactg gctggtcaac aaggtctttt ggttctgatg ccgtagattg actaagttca
    23221 cttaccttaa ggtctgtttt gtcggcagca aacttattgg tatcttctga tggatgatta
    23281 ctattgtcac ctgtttcttc ctcaacctgt gcgtttactg aagctgcttc gggaccattt
    23341 tccaccgaac ctgcgccctc tgaagaaacg tcctcttgtt gaaccgcctc aatcttgtct
    23401 tgctcattgg ctttttcaga aagctcctct ttctgagttg tttctgtcaa aagattttct
    23461 tctaactttt cctcagctac cttcggggtt tcttttttcc caaataaacg atcaaataat
    23521 cccattaact ctcctcttct aaaataaatg tcttcacagc ttctgcaact ccagactcat
    23581 cattggtcaa ggtggtcacg gcatcggcaa cggctttagc tcctgaaaca ccattagcca
    23641 tagctactcc aagtcctgcc cattctaaca tcgcaaagtc gttagcttcg tctcccatag
    23701 ccatgacatg tctagcgtca aggccaaggt gctggcaaag caagtttaat ccaaaagctt
    23761 tatcaatgcc ttttggcatg atttcaaaaa taatatctcg tgatttaaag gcttcaaaat
    23821 cctcaaataa gcttggggat agctttataa tctgctgatc caaaaagtct ggatcggtga
    23881 ctgtcacaat tttgttatag ataatgtctt ttggtagttc cgctacagat gtcacctcaa
    23941 taaaggtcaa cagtgggtta gctagatggt actgcgaatg cctaccgtcc ttacttggaa
    24001 tgctatacac atccccacca ctgataatat ctgttggtaa gccaaccgcc tctaaagctt
    24061 gctgaatctg gcaaacctgg tcaaaactga gagagctctt atccaaaacc tcccctgtat
    24121 ttcgctgaac caaaccccca ttaaaggtaa tactgtagtc atcgtggtca agcaagtcca
    24181 actcttctag caagttaccg atggccttta gagggcgacc tgtggtaata accaccttaa
    24241 cccctttttc tctaaccgct gctaaggctt ttttattggc atctgtgaca attttttcag
    24301 tgttgtagag ggtaccgtct aaatcaagcg ccaaaattct aatattcatg agttacacta
    24361 gtccttttaa atacgttagt actgctccgt cattacagtg gccaatgact tggtctgaaa
    24421 tcactttgat ttcaggtctt gcattttccg tagcaatagc cctaccagca aactccaaca
    24481 tctggtaatc attaaaatta tccccaaaag caatcgtttc tgctttctta atgcccaagg
    24541 cttgacaaag atgctccata ccaaagccct tattcacctc ttttaagata atatcaatgg
    24601 aatcaaagcc ggtggtcact gcagtggcat aagggagggc ttgattcaac caatcgcttc
    24661 cttctaagac ggtatggcct gtgaagttgg tagaaacttt aaaaatggcg tcattctcca
    24721 tatcttcaaa accattaata accttaacat tagcatagta atgttttgtt ttttgaatgt
    24781 actcttctga tgcgcctttt aaaatatagg ctgccttttg cccagaaaaa accatacctg
    24841 tttctacata gtgaggattg gctaaaatct tttttgcaac ttcggtatac tgctctttgg
    24901 tcatcatatc tgcaaataga atctcaccac ggtattgaac cacggaacca ttttctgcaa
    24961 taacagcaat ctgatccaaa aagggctcaa agagctgatc aatggctaag agcgagcggc
    25021 cactcgaaac cgcaaataaa atgccttttt cggctaactt tggcaagagc gcagctagtt
    25081 gctcttgatt ataagtacca tcttcagcta ggaaggtacc atccatatct gttgctatta
    25141 gttttatcat acaatgtctc ctttttttac tctagggggg tgcaaacgcc atttagggtg
    25201 tgcacttttt aacgatttaa ataagcccca tcatttctgc attctgttca atttttctta
    25261 ttatatcata aaaagcagct ttcattccat tcgtaaaaaa cagccagatt atatttcgat
    25321 aacctgaccg tttttgaagt gaactgctac tttttttatt tttataaatg accactggat
    25381 gtatactata aaagtagaca aaactttgtg tgttacaatc tgttttaaaa gacacaaaaa
    25441 cgaaaggaca acctatgtca acagttaaac gctacagatg aagaatttaa acaatccctt
    25501 gtcaaccttt atcaaactgg aaaaactcag tctgaactct gtaaagacta tggggtatcc
    25561 gcttctgcgc ttgcaaaatg gatcaaacag tattctcaag tcaaactcga agataattct
    25621 gtacttactg ccaagcaaat acaagagcta caaaaacgta atgcgcagct tggagaggag
    25681 aaccttatcc taaaaaaagc aagtgccata ttcatgcaaa acttaaagta agactcctcg
    25741 ctgtctatcg gttacgcttt gaacacgcca caacaacctt gtatcgtgtt ttacgtgtca
    25801 atcgctccac ttactataaa tttctaaaac ataagccctc aaaaagagaa ttggataagc
    25861 aaatttatag aaaacaaata cttgagattt ataccaaagc gaacaaaaga cttggtgtga
    25921 agtctatcaa ggtcattctt caaagagact acgacacaaa aatctctgaa ggaagaattt
    25981 accgtctaat gaagaatatg gcgctcccta aaatggctac cgttaagcca aaaacagctc
    26041 ttaaaaggac tcaaaaaacg tatcctcaaa acttactcaa ccagaaattt aatcctgaca
    26101 aaccaaatca agtatggtct actgacttca cctatatttc tattggatat aagaaatatg
    26161 tctatctctg cgcaatactt gatctctatt ctagaaaatg tattgcttgg aaattgagtc
    26221 atcatatgga tgcaaagtta gcatgtgaca ctctagaatt agctcttaat aaaagaaaga
    26281 ttgaaggaac acttctcttt cattccgacc aagggtcaca atttaaggcc aggaaattga
    26341 gaaaaataat tgatgacaac actatcatgc attctttttc taaacctgga tatccttatg
    26401 ataatgccgt aactgaagca tttttcaagt atttaaagca tagacaaatc aaccgaaaaa
    26461 agtatcaaaa tatcaaacag gttcaattag actgctttga atacattgag aatttttata
    26521 acaattacaa cccacatacg gctaatctag gactaacccc taatcagaaa gaagaaaatt
    26581 agtttaacgt aataaaataa cacggttttc tgtctactta tttgacatta gtccaaaata
    26641 catcctaatg gtgtcgcttt ttttatttca ctagacattc gttctagttt aggatataag
    26701 aaggtctcaa atttttttca ggatgtttat tccaaacatc caatcacctt tctatataac
    26761 tgttcattta gtggtataga caaacagaaa tactccgttt ctagtttcat agaagacgga
    26821 gtatttttta gtagagacgt ctggattgat agatagttgg tatatagctc atccctacta
    26881 ggctttcatt ataggctatg gtgggcttcg ttgacaacaa tatgtgatta ttattctaaa
    26941 tgacagtata catcaactat caagtatgct tttaatttta ttttttaaag ctacagatga
    27001 aaatctgtat tccatacttc catctaatcg aacaataata taatgatgtc tcgaatataa
    27061 ttcatgttta aaaaact
//
LOCUS       DAUCWO010000017        26966 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUCWO010000017 DAUCWO010000000
VERSION     DAUCWO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26966
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.017"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..289
                     /gene="gpsB"
                     /locus_tag="VGY20_000680"
     CDS             <1..289
                     /gene="gpsB"
                     /locus_tag="VGY20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ4273552.1"
                     /translation="QEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEALKAENEALK
                     KAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVFGKQIIE"
     gene            303..671
                     /gene="rnpB"
                     /locus_tag="VGY20_000681"
     ncRNA           303..671
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGY20_000681"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            734..1888
                     /locus_tag="VGY20_000682"
     CDS             734..1888
                     /locus_tag="VGY20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ4273553.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1902..3371
                     /locus_tag="VGY20_000683"
     CDS             1902..3371
                     /locus_tag="VGY20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273554.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLGILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKAMRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDAVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQEAQDQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3516..3998)
                     /locus_tag="VGY20_000684"
     CDS             complement(3516..3998)
                     /locus_tag="VGY20_000684"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ4273555.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4072..5403)
                     /locus_tag="VGY20_000685"
     CDS             complement(4072..5403)
                     /locus_tag="VGY20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEQ4273556.1"
                     /translation="MEIIGSLGVLVGVIVIIYLSVKEVNIIIAAPLATSLVIWFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYTTYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(5474..6253)
                     /locus_tag="VGY20_000686"
     CDS             complement(5474..6253)
                     /locus_tag="VGY20_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ4273557.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(6286..6945)
                     /locus_tag="VGY20_000687"
     CDS             complement(6286..6945)
                     /locus_tag="VGY20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ4273558.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINQDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6947..7597)
                     /locus_tag="VGY20_000688"
     CDS             complement(6947..7597)
                     /locus_tag="VGY20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ4273559.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREVVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(7621..8808)
                     /locus_tag="VGY20_000689"
     CDS             complement(7621..8808)
                     /locus_tag="VGY20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ4273560.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQIAIHSGIPSDKTAFTLDMVCGSGLKAIQLATQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGAGPIPATQKVLKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            8914..9858
                     /locus_tag="VGY20_000690"
     CDS             8914..9858
                     /locus_tag="VGY20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4273561.1"
                     /translation="MIDLAIAIKNHLCHNGINTIGKKTNRQGDNHLDIKQIRYFIAIV
                     ENHFNLSQAAELLYVSQPTLSMMINDFEKRENVKLFKRKRGRIIGLTYLGDNYYKDAQ
                     KVLSLYDDMFLKLHDHSKGLKGSINIGIPPLILSVVFSEVMPKLILENPGIQFNVKEI
                     GAYQLKNELLVGNVDVAVLLSPTGIADNLVETYEIQRSELSVCLSPRHRLASKKVIQW
                     EDLTDEQLALFDPSFMVHHLVLEACERHQVRPNIILTSSSWDFMLNSTKINHNVLTIC
                     PKPITELYQLKDIKCIPMERPISWRVVLTRLRKKVILK"
     gene            10032..11639
                     /locus_tag="VGY20_000691"
     CDS             10032..11639
                     /locus_tag="VGY20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ4273562.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11749..12384
                     /gene="gmk"
                     /locus_tag="VGY20_000692"
     CDS             11749..12384
                     /gene="gmk"
                     /locus_tag="VGY20_000692"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ4273563.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            12400..12717
                     /locus_tag="VGY20_000693"
     CDS             12400..12717
                     /locus_tag="VGY20_000693"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ4273564.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12782..15166
                     /locus_tag="VGY20_000694"
     CDS             12782..15166
                     /locus_tag="VGY20_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ4273565.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSLLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15228..16163
                     /locus_tag="VGY20_000695"
     CDS             15228..16163
                     /locus_tag="VGY20_000695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ4273566.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSN
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            16153..17475
                     /gene="rsmB"
                     /locus_tag="VGY20_000696"
     CDS             16153..17475
                     /gene="rsmB"
                     /locus_tag="VGY20_000696"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ4273567.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSHRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADTVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17513..18253
                     /locus_tag="VGY20_000697"
     CDS             17513..18253
                     /locus_tag="VGY20_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ4273568.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18250..20157
                     /gene="pknB"
                     /locus_tag="VGY20_000698"
     CDS             18250..20157
                     /gene="pknB"
                     /locus_tag="VGY20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ4273569.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQMPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTNQTDALQ
                     PPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQEL
                     YDVGLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKG
                     LDYQEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLS
                     VAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAI
                     VSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTE
                     LSPSETTAQTP"
     gene            20280..20972
                     /locus_tag="VGY20_000699"
     CDS             20280..20972
                     /locus_tag="VGY20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ4273570.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20969..21973
                     /locus_tag="VGY20_000700"
     CDS             20969..21973
                     /locus_tag="VGY20_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4273571.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            21966..22607
                     /locus_tag="VGY20_000701"
     CDS             21966..22607
                     /locus_tag="VGY20_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4273572.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNFQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22644..24044
                     /locus_tag="VGY20_000702"
     CDS             22644..24044
                     /locus_tag="VGY20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEQ4273573.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVEEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAANGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            24044..24421
                     /locus_tag="VGY20_000703"
     CDS             24044..24421
                     /locus_tag="VGY20_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ4273574.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24439..25380)
                     /gene="cysK"
                     /locus_tag="VGY20_000704"
     CDS             complement(24439..25380)
                     /gene="cysK"
                     /locus_tag="VGY20_000704"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ4273575.1"
                     /translation="MTKIYKTITELVDQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGMISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(25508..26140)
                     /locus_tag="VGY20_000705"
     CDS             complement(25508..26140)
                     /locus_tag="VGY20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ4273576.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNRQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            26196..>26966
                     /locus_tag="VGY20_000706"
     CDS             26196..>26966
                     /locus_tag="VGY20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4273577.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEII
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTL"
BASE COUNT         8609 a         4861 c         5551 g         7945 t
ORIGIN      
        1 gcaagaattt aagactagta tgcgagggtt tgataagaaa gaagttgatg aatttcttga
       61 taatgtaatt aaagattatg aaaatttcaa tgctcaaatt gaagctttaa aagctgagaa
      121 cgaagcctta aaaaaggcga agtttcaagc tagaaataca gtttcagcga ctgttcaaca
      181 accagtaccg caaccgactc gtgttgctca atcagctact aattttgaca tcttgaaacg
      241 tattagtaaa ttggaaaaag aggtttttgg taaacaaatt attgagtaat gaaaactgtg
      301 caatttttgg ataatcgcgt agtattttaa tactatgagg aaagtccatg ctagcactgg
      361 ctgtgatgcc agtagtgatt gtgctaggcg aacacataag cctagggatg tgcatacaca
      421 ttacggcgaa ggaaatggct aagtcgtttg atatgccaaa gtacttctga aagtgccaca
      481 gtgacgtagt ttttatggaa acgtaaaaaa tggaacgcgg taaacccctc aagctagcaa
      541 cccaaacttt ggtaggggca tgggatagtt ggaaacgaac aagctatcct gactgtcaac
      601 agacggtaga cagatgatta tcgaaggaaa taattcctag ttatttccgg aacaaaacat
      661 ggcttataga aaattgcata ataggttaag ctagcacttg ctagctttta tttgatttaa
      721 aagaaagaga tatatgaaag aaacatttag attagtcgca actgcagccg caggtttaga
      781 agctgtcgtt ggtaaagaag tcagagcact aggttttgat tgtcaggttg aaaatgggaa
      841 agtttatttt gaaggtgatg tagaagcgat tgttaaaaca aatctttggt tacgtgcagc
      901 agatcgtatc aaaattatcg taggacaatt tcctgctaga acctttgaag aattattcca
      961 aggtgtcttt gccttggatt ggggaaatta tttaccgcta ggagctaagt ttccaatctc
     1021 aaaagccaag tgtgtcaagt caaagctaca caatgagcca agtgttcagg cgattaccaa
     1081 aaaagctgtt gttaagaaac tgcaaaaaca tttccacagg ccagaaggag ttcctcttca
     1141 agaagttggg tcaacgttta atattgaggt atctatttta aaagaccaag ccactattat
     1201 gattgacacg acaggatcta gcctatttaa acgagggtac cgcgttcaaa agggtggggc
     1261 tcctattaaa gaaaatatgg cagctgctat cttggcctta tctaactggt ttccagataa
     1321 acctctggtt gatcccacct gtggatcggg aacgttttgc atcgaggcag ccctgattgg
     1381 gatgaatatt gctccaggat ttaaccgttc gtttgctttt gaagaatgga gctgggtaga
     1441 taaagacatg gtgcaacaag ttcgtgatga tgccgaacaa gaagctaatt atgaaatcga
     1501 attagatatt tctggctttg atatcgatgg ccgcatgatt gagattgcta aaagtaatgc
     1561 tgaagaagct ggtttatctg atgtgattac ctttaagcaa atgcgtctgc aagactttcg
     1621 aacagacaag gttaatggtg tagtgatatc aaatcctcct tatggagaac ggttgcttga
     1681 tgacaaagcg gttgacatct tgtataatga aatgggacaa acctttgcac cattgaaaac
     1741 atggagcaaa tttatcctta caagtgatga actttttgaa ctaaaatacg ggcaaaaagc
     1801 tgataaaaaa cgtaaacttt ataacggcac cctaaaagtt gatttgtacc aattttatgg
     1861 ggaaagggta aagcgacatc tgatatagag aggtagtaag cgtgtcagaa gaaagcaaag
     1921 aagtcgaagt aacaaaagaa tcgcaaacat tggggcttaa tgaagccaag tcaatgacaa
     1981 ttggtgaagc tgtacgcaaa cagtcagaga taaaagcagg tgttactaag gatgatagta
     2041 ttttagataa gtacattaag caacaccgtg acgaagtttc ttctcaaaag tttgatgcta
     2101 agtatacaga gcttgataca gctagtttag ataactttat caaaaaacaa agagaagcat
     2161 tgagtaaagc cggtcttgtt gatgacgaac ctgtgtctgc ggagtctgct gagcaagata
     2221 gcactttggt tgaagaagta gctgaagact tagctccaat ggagactact gcggtggtaa
     2281 ctgggattcc ggtggaagcg acagtccctg tgttagatct agatccatca gagagggtca
     2341 ttccagaacc acagatgacc aaggaagaac caaaaagaga tcagtttctt tctgaagata
     2401 gccaccatcc agccaaacaa aacacgaaaa aaggttggtt aatagcttta tttctgctat
     2461 tattgggcat tcttgcagtt gtatttggtt ggaaccactt cttgagacaa gacagtggta
     2521 aaacaactca aacagcaagt aaacagacaa agacctcttt gcagaccgac agtgctaaaa
     2581 aagcaatgcg cttaaaggct gctgctaaag ctttcgaaaa acaatatggt actttttata
     2641 cagatgccac aaagagcaaa ttaaaaaata gtgcctttgc tactttgcca gatttagaag
     2701 ctgccttgaa agctttggaa ggctctgctt actatgataa ggcgaaagca aaagtcgatt
     2761 cccttaagaa agctattgct gctattacag cggttaacgg taaatttgtt tccgatgctg
     2821 ttgtagatgg tgaaaaggtt tcggctgaag tcaaggctga tgctaatttt gatgatttat
     2881 caagtgcaac cttgacaaca ggaaatgcaa atcttgatgc tgtcttgcag gcaagcatca
     2941 cagaaggtcg tcagcaacta gccagcaagg ctgaggccgc taaagcagct aatgagcaag
     3001 aagcccagga tcaagcagct caaggacaaa gtacatcagt agcaccatct ggctatggct
     3061 tgacaagtta tgatcctgca agcttacaac ggcatttatc acgtgtacct tacaatcaag
     3121 atgttattgc tgacagagct aacccatctt gggcatttaa tccaggagtt cttgaaaaaa
     3181 ttgtagcaac ctcacaagct agaggttata tttcaggcaa ccaatatatt ttagagccgg
     3241 ttaatattat taatggcaat ggttactata atatgtttaa gcctgatggc acttatctgt
     3301 tctctattaa ttgtaagacg ggttattttg taggaaatgg caaaggttat gctgatgctt
     3361 tagattactg agaaaatcaa aaaaactgag ataaggcaac ttgtctcagt tttttgatta
     3421 ataaagtatg ttgtcttaaa atctgcagat agacttgttt tttgtaacaa gtcaaatagg
     3481 tcagtaggat tgttatgttt gacaacacac tagtatcaga tgacatggcg actaaaagga
     3541 tcgtctgaaa tcccttgatc aataatcaat tgagcccatt ctttggcggc aaaaaggcta
     3601 tgatccttat agtttccaca ggattcaaga gttgttccag gaacatcttc ccaagtaatc
     3661 ccagttgcaa tttcttctag gctagattta atcaccttgg caatatcagt agaactgtgt
     3721 tttccccaca tgataaggtg aaaacctgtt cgacagccaa aaggagagca atcaatcatc
     3781 ccatcaatgc gttggcggat gagcttggca agtaagtgtt caatggtatg caaaccggct
     3841 gtttcaatag aattttggtt aggctgcact aggcgaacat caaaatttgt aatacgatcg
     3901 cccttgggtc caaattcttc agaaataaga cgaacataag gggcctttac aatagtatga
     3961 tctagctcaa aactttcgac gataacttct tttgtcattt caatctcctt tgttttacac
     4021 tttatcataa cacaaatagt gagaaagtgg ctacttgtgg aggagaaaag gctaagaaaa
     4081 cagatcaaag gctatcataa tgacctgtgc gataaaagta cttacagaaa cagtaataaa
     4141 ggtttcttta aaagcatttt tgtggttaag tcctgtcaat gctaagaagg tgagaaagac
     4201 accactttgg ggcacaatgg taaagatatt cgaagcaata gtggcaacac ggtgaatcat
     4261 ttcgggatta aggccttgat ctaaataata ctgggcaaag ttaggcatga caattcctag
     4321 agcacctgaa gaagaaccgg taatagctga catagaggaa gtcaacacgg ctaaactaat
     4381 aagaggatta ccgggaatat tcaaaatgag atctgagaag aatgtgaaac ctggggcaat
     4441 catcacaacg gctccgaagg caactgcaga tgctgtcgca aaaataggtg caatagagct
     4501 gctagctcct aaattaaaga cagcaatctt attaggaata aactgtctaa agagccagct
     4561 tgcggtgaga atggcagcta aaagtgctat gaaaataatg ttcttcttaa aaaaatcgtt
     4621 tccgaaaagg ctaccggtta gggcgataat aatgagcaac aataaaggta agatactggc
     4681 aaggaaatgt gggagatttt ttgttttcac ttgtatctca ttgtcaaaag cataagtggt
     4741 ataagtttca ccccttgcca tactttttgc tagacaatat ttcatataaa agagcccaaa
     4801 agtcacgcag ccaattgagc caacgatact cggaatggcg gcagcagtta gtgaggtgtc
     4861 caagtattga ataggaataa cattttgaat ggcaggtgtt ccgggtaaaa tcgtcatggt
     4921 aaaagtcgca ataccgagcc aaagaggaac ttgaattaag ttccaagcca agtccatttt
     4981 tttgaataga cttcgtgcaa gcggcaagac agcaaacatg acaacaaaga ggctgattcc
     5041 cccgtaagtt aatatagcac tgatcagaaa aatagcaacg agtactttat aaggactatc
     5101 atgaccaact ttttttaaaa tataatctgc aatagaagtg gttgcaccgc ttgtttccat
     5161 taatttggct aagatagaac ctaataaaaa gatagcaaaa taattgagaa tataagtgga
     5221 gagtgccccc ataaattggt tgggttcttt tccaagtaag gttgtggttg gatccatttg
     5281 attaaaccaa atgaccaagc tggtagcaag aggcgcagcg atgataatgt taacttcttt
     5341 gacagacagg taaataataa caataacacc taccaagacc cctagagaac caataatttc
     5401 catagtttta tgacctcctt attagaaaat aaagagacag atcacaagat acagcctctt
     5461 tatctctcca aatttattga gcagtgtagc caccgtctaa gatacaggct tgacctgtaa
     5521 caccttttgc cttatcactg gcaaggaaag acacatagtc tgcaatttct tgaacgtcaa
     5581 tgaggcgttt ttgaggaaca agtgggtata gcacttcttc aagaacattt tcaaggggaa
     5641 tacctcttgt tttggaaagg tcttcaaact ggccacgaac cagtggtgtg tcaacatatc
     5701 caggacaaat ggcattgacc gtaatgcctg aatcagcagc ttctaaggca gttactttgg
     5761 tcagaccgat caagccgtgc ttagctgaat tgtaggcgga tttgccagca aaaccaatga
     5821 caccattgat agaagccata ttaataatac gaccgtgttt ttgagctttc attgtaggaa
     5881 aagcacgttt aatggcaata aatggtgctg tcaacatgat tttaatcatg aattcaaatt
     5941 tttcagttgg aaaatcttca atcatggcaa catgttgaag gcctgcgttg ttaaccaaaa
     6001 tatcaatacg accatatttt tcaacaaccg tatcaatagc agcattgatg gcttcttctt
     6061 tggtgacatc gcacacaaca ctaaaagcgt catagccttc tttagtatag tcagcaacaa
     6121 cctgatccag tttttcttga ttaatgtcag aaaaaacaac cgttttacct tcttttaata
     6181 aggtttcccc gatttgtttt ccaattccgc ttgcagcacc agtgataaat gcaacttctt
     6241 tagtatgtgt catgatacta tcctcgtctt tcttttggtt agtctttagt ttgttttatc
     6301 aattagggta acttcggtaa cgttttgcac gtcttcaaag gtgtaatctt ggttaatttc
     6361 aagggcgcat agaccttcat cttgatattc aaagactccc atttcagtaa tgattaggtt
     6421 aacaacattt tgagcagtca gaggaagggt gcatttgtct aaaatttttg ctttgccttt
     6481 attggtatgc tccattgcaa caatgacttt tttagcacct accaataagt ccatggcccc
     6541 tcccatccca ggaaccattt ttccaggaat caggtaatta gcaatgctag cttctttatc
     6601 aacttgtaag gcacctaaga cggttgcggc cacatggccg cctcgaataa taccaaagga
     6661 agttgagcta tcgaagaaag cccctccagg agcaatacta acaggttgcc caccagcatt
     6721 aacaatacta ggatcgtagt gatcatctgt taatccagtg agaccgacaa aaccattttc
     6781 ggactgcagg gtaatggtta caccttcagg aatatagttg gctacttttg ttggcaaacc
     6841 tatgcctaag ttgaccagtg tattgtgttc taattcttgt gctacacgct ttgcaatacg
     6901 tgtttgaatc tcttcttttg aaagaacagt agctgatgtt cccatgttag gctcctttca
     6961 ctaagtagtt aacaaaaatg ccaggagtat ggacaaaatt aggatccatt tgccccacgt
     7021 caacaacttc tcttgcttca acaatggtgg ttttcgcatt agcagccatc acatgattaa
     7081 agttattttc agaacctgcg tattgcaaat tgccattttt atctgccttg ttagcaaaaa
     7141 taagtgcgat atcagctttc aaaggttttt caagaaggta gtctttgccg tcaatagtaa
     7201 tgacttcttt tcctttggca acttcggttc ctattccagt aggtgttaag aaaccgccta
     7261 gcccaaaacc gccactgcgg atgcgctcag ctaatgttcc ctgaggtaca agatcaatta
     7321 cagtctcgcc ctcagtcatt tgtcggcccg cttcacggtt gagaccgata tgtgatgcaa
     7381 taatagttga aaactgttta ttggcaacca tcttaccaac gcctttgtca ggaaaaccgg
     7441 catcgttaca gattaaagtg aggtctttga cacctttttc cacaagtgca tcaattaatt
     7501 tttctggtgt gccatttgtc atgaagccac caaccataat ggtatcacca tctttaacgt
     7561 gagctaccgc ttcttttatt gtgatttctt tacacatttt ttctaccaac tttcacgaga
     7621 ctaagtattg tttttcacga taacagcagt tccttgtcct ccaccaatgc aaagtgttgc
     7681 tagtcctctg gttacttggc gttttttcat ttcgtggatt aatgtgacta gaatgcgagc
     7741 gccactggca ccaataggat gacctaaagc gatggctcct ccattaacat tcacgatttc
     7801 tgggtttaac tttaaatctt ttaaaaccgc caaggactgt gctgcaaatg cttcattgga
     7861 ctccactaag tccaaatcag aaattgttaa tccagctttc ttaaggactt tttgagtggc
     7921 aggaattggt ccagctccca tcagttcagg tgcaacacct gcactagcat agctttcaat
     7981 agtgactaaa ggtgttaacc ctaattcaag agctttttct tcagtcatta acatcaataa
     8041 ggctgcacca tcgtttattc ctgaggcatt cccagcagta acggttcctt ctttagggag
     8101 gaaggcagga cgtaattgct gtaatttttc aaggcttgtg tcaacttttg ggtattcatc
     8161 tgttgtaaca attaaagggt ctttgcgtcg ttgaggcaca gagacaggga caatttcgtc
     8221 agcaaaacga tgattttcta atgccttagc agctttttct tggctttcta aagcaaattg
     8281 atcttgctct tctcttgaga tgccatattt ctgagcaacg ttttcagcag tgatacccat
     8341 atgataattg ttgaaagcat ctgtcaatcc atcatgaaca agagtatcaa tcaattgact
     8401 attaccaagt cgctgtccaa aacggtgctg agttgacaca taggctgcct gtgacatgtt
     8461 ttcgacacca ccagcaataa cgatatcagc gtcaccaagc atgatgcttt gcgtagctaa
     8521 ttggattgct ttaagtcctg aaccacagac catatcaagc gtgaaggctg ttttatcact
     8581 agggatgcca gagtgaatag caatctgtct ggcaacgttt tgccctaatc ctgcatgaag
     8641 gacgtttcct aggatgactt catcaaccat ttcaggcttt acttgcgttt tatcaaggat
     8701 ttttttcagt acagttgttc caagttcaac agcagagaca cttttaaagg agcctccaaa
     8761 agctccgata ggagttcgta aagcttcaac gataactatg tttttcataa aataaacatc
     8821 ctttcatttt tagaaagcgt tttctgtatc tctaagataa caagaaaccg ctcaaaataa
     8881 aaatatagaa tttttataag catataaatt tttttgatag atttggcaat agcaattaaa
     8941 aatcacttat gtcataatgg aattaatact atcggaaaaa agactaatag acaaggggat
     9001 aaccatttgg atattaaaca aattcgttat tttatcgcaa tcgttgaaaa tcattttaac
     9061 ttgagccagg cggcggaact gttgtatgtg tcgcagccga cacttagcat gatgatcaat
     9121 gattttgaaa aaagggaaaa tgttaagctc ttcaaacgaa aaagaggtcg tattattggg
     9181 ttaacctatc ttggtgataa ttattataag gacgctcaaa aggttctcag tctctatgat
     9241 gacatgtttt tgaaattgca tgatcacagt aaagggttaa agggaagtat caatattggt
     9301 attcctcctc ttattttatc agttgttttc tcagaagtaa tgccaaaact gattttagaa
     9361 aatccgggaa ttcaattcaa tgttaaggag attggtgctt accaactaaa aaatgaacta
     9421 ctcgtgggaa atgttgacgt cgcagtcttg ttatccccaa caggcatcgc tgataatttg
     9481 gttgagactt atgaaatcca gagatcagaa ttatctgtat gcctgtctcc acgtcatcgt
     9541 ttagcttcca aaaaagttat tcagtgggaa gatttaacag atgaacaact tgccctattt
     9601 gatcctagtt tcatggttca ccatttagtg cttgaagctt gcgagcgtca ccaagtcaga
     9661 cctaatatta ttttgacgtc ctcttcctgg gattttatgc ttaattctac caagattaat
     9721 cacaacgtct taactatttg tcccaaacct attacagaat tataccaatt aaaggacatt
     9781 aagtgtattc caatggaacg tcccatttct tggcgtgttg tcttaacacg cctccggaaa
     9841 aaagtaattc tgaaatagaa gcctacatca tggacgactt gctacagtct ttttttaagt
     9901 agggttctca accacttggc ccagatgtta atttttttaa aaatgaattg gaattgtgta
     9961 aacgctcaat ttatgataaa ctaagaggtg gctttctatt aatgaaatct agaatatatt
    10021 gaggtacaaa catggttaat attattttat taattgtttc tgccctcatt ggtttaatat
    10081 taggttatgc acttatttcg attagactca agtctgcgaa ggaagctgca gagttgactc
    10141 ttttaaacgc tgaacaagaa gctgttgata ttcgtggcaa agcagaagta gatgctgaac
    10201 acatcaaaaa aacagctaaa cgtgaaagta aagcaaatcg taaagaatta cttttagaag
    10261 caaaagaaga ggcaagaaaa tatcgtgaag agattgaaca agaatttaag tctgaaagac
    10321 aagagcttaa acaactcgag acacgcttag cggagcgctc cttaactctt gaccgtaaag
    10381 atgaaaacct atcaagtaaa gaaaaggtac tagatagtaa agaacaaagt ctgaccgata
    10441 aatctaaaca cattgatgag cggcaacttc aagtggaaaa acttgaagag gagaaaaaag
    10501 cagaactgga aaaagttgct gcgatgacga ttgcagaagc gcgtgaagtg attttaatgg
    10561 agacggaaaa caaactgacc catgaaattg cgacccgcat tcgagatgcc gaacgtgaca
    10621 tcaaggaccg aacagttaaa acagccaagg acttgttagc gcaagccatg caacgtcttg
    10681 ctggtgagta tgtgactgaa caaactatta ccagtgtcca tctcccagac gacaacatga
    10741 agggccgaat tattggacgt gaaggccgta atattcgtac tttagagagc ttgactggca
    10801 ttgacgttat tattgacgat actcctgaag ttgttatctt atcaggattt gatcctattc
    10861 gacgtgaaat tgctcgtatg accttggaat ctctgattgc tgatggtcgc atccatccag
    10921 ctcgtatcga ggaattggtt gagaaaaatc gtcttgaaat ggataatcgt attcgtgagt
    10981 acggtgaagc tgcagcctat gagattggtg caccaaacct tcatcctgat ttgattaaaa
    11041 tcatgggacg cctgcaattc cgtacctcgt ttggtcaaaa tgtcctacgt cactctgttg
    11101 aggttggtaa gttagctggt attttagctg gtgagttagg tgaaaatgtt gctcttgccc
    11161 gccgtgctgg tttcttgcat gatatgggta aagctattga ccgtgaggtt gaaggtagtc
    11221 acgttgagat tggaatggaa tttgcacgta aatacaaaga acatccagtt gttgtcaaca
    11281 ctattgctag ccaccacgga gatgtggagc cagattctgt gatcgctgtg ctagtagctg
    11341 cagcagacgc tctcagttcg gctcgcccag gcgctcgtaa tgagtcaatg gagaattaca
    11401 tcaagcgtct tcgtgattta gaagaaatcg cgacaagttt tgatggggta caaaatagtt
    11461 ttgctctaca agctggacgt gagattcgta ttatggttca acctgaaaaa atttcagatg
    11521 atcaggttgt cattttgtcg cataaagtaa gagaaaaaat tgaaaacaat ctagattacc
    11581 caggaaatat taaagtaact gttattcgtg agatgagagc ggttgattat gccaagtaga
    11641 aaaagaggaa ttatcctctt tttctttatg acttaccttt gaaaatgttc ttgaaaaaga
    11701 ctagcctttt tgataaacta ttaaaaagca actaataagg agaatacaat gtctgaacgc
    11761 ggtttgttaa tcgtcttttc tggcccttca ggtgtcggaa aaggaacggt aagacaagaa
    11821 attttttcaa cgccagatca taaatttgaa tattcggttt caatgacgac tcgtccgcaa
    11881 agaccgggcg aggttgatgg ggttgattat ttcttccgca cacgtgaaga atttgaagaa
    11941 ctcattaaaa ctggtcaaat gctagagtat gctgaatatg ttggtaatta ctatggcacc
    12001 cctttaactt atgttaatga gactcttgat aagggaattg atgtcttttt agaaattgaa
    12061 gttcaaggag ctcttcaggt taagtcaaaa gtaccagatg gtgtttttgt ttttttaaca
    12121 ccaccagatt tagatgaatt agaagaccgc ctggttggtc gtggtacaga tagtcaagaa
    12181 gtcatagctc aacgtattga aagagcaaaa gaagaaattg cccttatgcg cgaatacgat
    12241 tatgcagttg ttaatgatga agttgccttg gcagctgagc gggttaagcg tatcattgag
    12301 acagaacact ttcgtgtgga gcgcgtgatt ggtcgttatg ataaaatgat taaaataaca
    12361 aaaaatccct ttaaagctaa atagaaaaga gtaagcatta tgatgttaaa accttccatt
    12421 gataccttat tagataaagt accatctaag tattcacttg tgattttaca agccaaacgt
    12481 gcccatgaac tagaagctgg tgccactcca actcaagaat ttaaatcagt gaagtcaaca
    12541 cttcaggcac ttgaagaaat tgaatcagga aacgttgtga ttcacccaga tccttcagct
    12601 aaacgtgaag ctgtccgtgc taagattgag gcagaacgct tggccaaaga ggaagaggaa
    12661 cgtaaaatca aagaacaaat tgccaaagaa aaagaagaag aaggagaaaa aatctaaggt
    12721 ttcagaaatc ttagattttt tcatatttag tacgatgtcc atgacagaaa aggagaagca
    12781 aatggaaaaa gtagctcatg tgattgtaga tattccattg atgcaaacgg acaagccatt
    12841 ttcttatggt attccaaaag agttagtttc tctcgtgcag cttggttctc gtgtgcatgt
    12901 tccttttgga aaagggaatc gtttgttgca aggctttgtt attggttttg gccaggaaga
    12961 ttcaagctct cttaaactga ttcagaccgt tttagatcct gagccagtcc taaaccaaga
    13021 acaattgacc cttgctgatc agcttcgaaa aacagttttt tcttataaaa tcacgctctt
    13081 aaaagcaatg attcctaatc ttcttaactc taactatgat aaagtgctaa ggccagagtc
    13141 aggtctaaaa aagcctgata gagactttct ttttgaagga aaaccatctg tcttatattc
    13201 taccttagac cgcgagaagg aaaaaatagc tcttaaggcc atacaagctg ggcatattac
    13261 tgttagttat ttagccaaag acaagaaaaa cctaaagaca gaaaaatatt atcacgtcga
    13321 cttagacgcc ttagcggtac atcccatctc tagtcgtgcc aaaaagcgtc aattattaaa
    13381 agactatctg ctaacccata cgaaggaagc taaacttgca acgctttatc aagccttttc
    13441 tcgagatgtg gtggcttact ttgtgacaaa tcacttaatt cgtattgacg aacgtcaaat
    13501 tgatcgcagt gagtcctatt ttgaccagat caaaccatct tcttttttaa ccttaaatga
    13561 acagcaagcg tcagctgtta ctgaaattgt tgagcaaatt gggaaaccga gtaaaccttt
    13621 tttgattgaa gggattacag gttcgggtaa gacagaagtt tatcttcata tcatcgaagc
    13681 agttttaaaa caggataaga cagccattgt tttagtacca gaaatttcgt tgactcctca
    13741 gatgacctct cgttttattt cccgtttcgg aaagcaagtg gctattatgc attcaggctt
    13801 atcagatggt gaaaagtttg atgagtggcg caagataaaa acaggacagg ctaaggttgt
    13861 ggttggtgcc cgctctgcta ttttctcccc actagagcgg attggtgcaa ttattattga
    13921 tgaggagcat gaatcaacct ataaacaaga aagcaaccct agataccatg ccagagaggt
    13981 tgccctgctc agagctaaac accatcaagc agttgtggtt atgggctctg ctacgccaag
    14041 tattgaaagc cgagcaaggg ctagtaaggg ggtctatcat tttattcaat taacgcaaag
    14101 agcaaatcct cttgctaaga ttccagaagt aacgattgtt gattttcgtg attatattgg
    14161 acagcaagca gttagcaatt ttaccccata tttgattgat aagattaaag aacgtttggt
    14221 taaaaaagaa caagttgtct taatgttaaa tagacgtggt tattctagtt ttgtgatgtg
    14281 tcgggactgt ggttatgtgg acaaatgtcc caactgtgat atctccttaa cccttcacat
    14341 ggatactaaa actatgaatt gccattactg tgggtttcaa aaacctatcc caattacttg
    14401 tcctgagtgt catagcaaca gtatccgcta ttatggcact ggaactcaaa aagcttttga
    14461 tgaattgcaa ggggttatcc cagaagctag gatcttgaga atggatgttg acaccacacg
    14521 aaaaaaaaga agtcacaaaa ccatactaga tagttttggt cgccaggaag cagatatttt
    14581 gttagggacg caaatgattg ccaaaggact ggattttcca aatgtgactt tagttggggt
    14641 actaaatgca gatacctcct taaatttgcc agattttcga gcgtcagaaa aaacgtttca
    14701 attgttaaca caggttgctg gtcgggcagg ccgtgcccat aagccaggag aagtcttgat
    14761 tcaaacttat aatcccgatc actatgctat tcaattagct aagaaacaag attttgaagc
    14821 cttttaccgt tatgaaatgg cgattcgccg tcagatggct tatccccctt actactttac
    14881 agtgggtatt acgctttcgc atcgtttaga agcatcggtt gtaaaaaaag cttatcaagt
    14941 tacagaactg ctaaagagtc tcttatcgga taatattaaa atattaggac cgactcctaa
    15001 gccaattgct agaacacaca atttatacca ctatcaaatt ttgttaaaat accgttttga
    15061 agataaccta gaagagacgc tcaatcgtat tcttgattgg agtcaagaag cagataatcg
    15121 tcatttaaaa ctaattattg attgtgaacc gcaacaattt ttataaggta aatcactaaa
    15181 tctaggatga tcagttatga tataataaaa atacaaagag gaaagtcatg ataaaattat
    15241 tatttatggg aactccccaa ttttcagcaa ctgtattgaa gggcttgcta gataatcctg
    15301 cctatgagat tttaggagtg gttactcagc ctgatcgagc tgttggacgc aaaaaagata
    15361 ttaaggtaac acctgtaaag caattagctc ttgagcatgg aatatcaatc tatcagccgg
    15421 aaaaattatc aggctctcaa gaactcattg agattatggg acttggtgca gatggtatta
    15481 ttacagcagc atttggccaa tttttaccaa cactattact tgattcagtt tcatttgcta
    15541 ttaatgtaca tgcatcattg ttacctaaat atcgaggtgg agcacctatc cattatgcta
    15601 ttatgaatgg agataaagag gctggcgtta ctattatgga aatgattaaa gaaatggatg
    15661 caggtgacat ggtcgctaaa gcaagcactc caattcttga aaccgataat gtaggtactc
    15721 tttttgagaa attagccatc attggtcgag acttactttt ggatagtttg ccagcttatc
    15781 tttctggaga attaaaacct attcctcaag atcacagcca agctactttt tcacctaata
    15841 tctctccaga acaagaaaaa ttagattgga caatgtctaa tcaagaagtg ttcaatcata
    15901 ttcgaggtat gaacccttgg ccggtagcgc atacgttttt agaggggcaa cgtttaaaaa
    15961 tttacgaagc tcaactagct gaaggagaag gattacctgg acaagttatt gtcaaaacca
    16021 aaaaatcttt ggtaatagct actggacaag gagctttgtc cttgatcgtt gtgcaaccgg
    16081 ctggtaaacc taaaatgtca ataatagact ttttaaacgg cattggtaga aaacttgaag
    16141 taggggacat cattggcaga taattggaaa aaaagtacgc gaggcaaagc cctccttgtc
    16201 attgaagcta tttttgatca gggggcttac acaaacatag cgcttaatca gcagctaagc
    16261 aataaagctt tatctgctaa ggacagagct ctattaactg agattgtata tggtaccgtt
    16321 tcacgtaaaa ttagcttgga atggtattta gcccattatg tcaaagaccg agataaattg
    16381 gacaaatggg tttattatct ccttatgctc agtctttatc aactgattta tttagataag
    16441 ttaccagcgc atgccattgt taacgatgcg gttggtattg ccaaaaatcg tggcaataaa
    16501 aaaggtgcag agaagtttgt caatgctatt ttacgtcaat tcaccagcca tcctctccca
    16561 gatatggaaa caattaagcg tcgaaataag tattattcgg tcaagtattc actgcctgtt
    16621 tggttggtta aaaagttaga agaccaattt ggctcccaca gagcagtggc tattatggag
    16681 agtttatttg tgcgtagtaa ggctagtatt cgcgtgacgg accctttgaa gttagaagaa
    16741 gttgctgagg ctttagatgc agaacgttcc cttttatcag caactggttt aaccaaagcg
    16801 tcaggtcatt ttgcagccag tgattatttc acaaatgggg acatcactat tcaagatgaa
    16861 agcagtcagc ttgtagctcc tactttaaac attgacggtg acgacataat tcttgatgcg
    16921 tgtagcgctc cagggggtaa gacctctcat atagcatctt acttaaaaac aggtaaagtt
    16981 attgccttag atctctatga tcataaacta gagttggtga aggaaaatgc taaccgctta
    17041 ggagttgctg ataacattga gacaagaaaa ctagatgcca gagaggttca tcgtcatttt
    17101 gaaaaggata gctttgataa gattttggtg gatgctcctt gttcaggaat tggtttgatt
    17161 cgtcgaaaac ccgatatcaa atataataaa gaaagtcaag attttaatgc tctacaggct
    17221 atccagctag agatattgtc tagtgtttgt caaaccttgc gtaaaggtgg tataataaca
    17281 tacagtactt gcactatttt tgatgaggag aatcgtcaag tcatcgaagc atttttacag
    17341 agtcatccta attttgaaca agtaaaactg aaccatacac aagcagatac tgtcaaggat
    17401 ggttatctta ttatcacacc agaacaatac cagactgatg gcttctttat tggacaagtc
    17461 agacgagtct tgtagtattt taggaggaaa ctgactgagt cagaaaaaac gtatgaagat
    17521 ttcattaaaa acagacattg ggcaaaaacg ctcaaacaat caagatttta tcaataagtt
    17581 tgataataaa aaaggcatca ctttagtgat tttagcagac ggtatgggtg gccacagagc
    17641 tggaaatatt gccagtgaga tgacagtcac agacctggga agagaatggg taaaaacaga
    17701 cttcacagag ttaagtcaga ttagagattg gttatttgaa actatccagt cagaaaatca
    17761 acggatttat gatctaggcc aatcagaaga ctttaaagga atgggaacga ctgttgaagc
    17821 agtagcttta gttgaatcta gcgccattta tgctcatatt ggtgattcac ggattggtct
    17881 tgtccatgat gggcattata ccttattgac cagtgatcat tctttagtga atgagttggt
    17941 taaggcagga cagatcacag aagaagaagc tgctagccat ccacaaagaa atattatcac
    18001 tcaatctatt ggtcaagctt ccccagtaga gcctgattta ggagtgagag ttttggagcc
    18061 aggggattat cttgtcatta acagtgatgg tctaaccaat atgatttcaa atgatgaaat
    18121 tgtgactatc ttgggcagta aggttagtct tgatgaaaaa aaccaagaaa tgattgactt
    18181 agctaaccta agaggaggcc tagataatat tacaatcgct ttggttcaca acgaaagtga
    18241 ggacgtcgaa tgattcagat tggcaaatta tttgctggtc gttatcgcat tctgaaatct
    18301 attggccgcg gtggtatggc ggatgtttat ttagcaaatg atttgatctt ggataatgaa
    18361 gacgttgcaa tcaaggtctt gcgtaccaat tatcaaacag atcaggtagc agttgcgcgt
    18421 ttccaacggg aagcgcgggc catggctgaa ttgaaccatc ccaatattgt tgccatccgg
    18481 gatataggtg aagaagacgg acagcaattt ttagtaatgg aatatgtgga tggtgctgac
    18541 ctaaagagat acattcaaaa tcatgctcca ttatctaata atgaagtggt tagaattatg
    18601 gaagaagtcc tttctgctat gactttagcc caccaaaaag gaattgtaca cagagattta
    18661 aaacctcaaa atatcctact aactaaggag ggggttgtca aagtaactga tttcggcatc
    18721 gcagtagcct ttgcagaaac aagcttgaca caaactaatt cgatgttagg cagtgttcat
    18781 tacttgtctc cagaacaggc tcgcggctcc aaagcgacga ttcaaagtga tatttatgcg
    18841 atggggatta tgctctttga gatgttgaca ggccatatcc cttatgatgg cgatagtgct
    18901 gttacgattg ccttgcaaca ttttcaaatg cctcttccat ctattatcga ggagaaccac
    18961 aatgtgccac aagctttgga gaatgttgtt attcgagcaa cagccaagaa attaagtgat
    19021 cgttacgggt caacctttga aatgagtcgt gacttaatga cggcgcttag ttataatcgt
    19081 agtcgggagc gtaagattat ctttgaggat gttgaaagta ccaaacccct ccccaaagtg
    19141 gcctcaggtc ccaccgcttc tgtaaaattg tctcccccta ccccaacagt gttaacacag
    19201 gaaagtcgat tagatcaaac taatcaaact aatcaaacag atgctttaca gccccccacc
    19261 aaaaagaaaa aaagtggtcg ttttttaggt actttattca aaattctttt ttctttcttt
    19321 attgtaggtg tagcactctt tacttatctt atactaacta aaccaacttc tgtgaaagtt
    19381 cctaatgtag caggcactag tcttaaagtt gccaaacaag aactgtatga tgttgggcta
    19441 aaagtgggta aaatcaggca aattgagagt gatacggttg ctgagggaaa tgtagttaga
    19501 acagatccta aagcaggaac agctaagagg caaggctcaa gcattacgct ttatgtgtca
    19561 attggaaaca aaggttttga catggaaaac tacaaaggac tagattatca agaagctatg
    19621 aatagtttga tagaaactta tggtgttcca aaatcaaaaa tcaaaattga gcgcattgta
    19681 actaatgaat atcctgaaaa tacagtcatc agtcaatcgc caagtgcggg tgataaattt
    19741 aatccaaacg gaaagtctaa aattacgctc agtgttgctg ttagtgatac gatcactatg
    19801 cctatggtaa cagaatatag ttatgcagat gcagtcaata ccttaacagc tttaggtata
    19861 gatgcatcta gaataaaagc ttatgtgcca agctctagct cagcaacggg ctttgtgcca
    19921 attcattctc ctagttctaa agctattgtc agtggtcaat ctccttacta tggaacgtct
    19981 ttgagtctgt ctgataaagg agagatcagt ctttaccttt atccagaaga aacacactct
    20041 tctagtagct catcgagttc aacgtcaagt tcaaacagtt cttcaacaaa tgatagtact
    20101 gcaccaggta gcaacactga attaagccca tcagaaacta ctgctcaaac accttaacaa
    20161 agaatagtta gtctcatgtt ttactagcat gccactaact attcttttta actactcgtc
    20221 atataaagat gcaattttcc aggaattctg ataaaataca aagaaataag gggaaactaa
    20281 tgaaaaaatt tcaattcttt ttacttatcg agtgtatact gctagctatg ggtatcatga
    20341 caatcttgga taatgattta tcaagtttta tcctaattct agttcttatc ttattagcat
    20401 tacgctttta taaccaagat agccgcaata attttttgct aactgtcagt ttattgtttc
    20461 tttttttaat ttttatgctt aatccctaca ttatcatggc tgttcttttg gggatagttt
    20521 acattttcat taaccatttt tcacaagtca aaaagaagaa tcgctttgcc ttgattcgtt
    20581 tcaaggaaga aaagattgaa gtgaataata ccaagcatca atggattggt actgctaatt
    20641 atgaaagtga ttattattgt tttgatgaca ttaacatcat tcgaatctca ggaaatgaca
    20701 cggttgactt aaccaatgtt attgtgacag gaatggataa tatcattgtg atacgtaaaa
    20761 tttttggaaa tacgactata ttggtaccta ttgatgtcac tgttacttta gatgttagtt
    20821 ccatatacgg aagcgttgat ttttttagat gtcagcaata tgatttgcgc aacgaatcta
    20881 ttaagttaaa agaaacggat aatcaatccc ttaaaaaagt caaaattatc gttacaacta
    20941 ttgccggaaa tgttgaggtg gataggcgat gaaaaaacgt tactatgctc ttgtttggct
    21001 ctactcaacc attaccattt tatctattgt ttttgtggtc atggataatt taggaatcac
    21061 ttttaactac cttcgcaatc atttatggca ggtcgaacgt ctagggtttt cgattttatt
    21121 gttgattgtt tctgtgactt tattattgtt gttattgtgg attattatgg atgataacag
    21181 taagcgtaac atcaaccaaa atctaaaata tattctcaat aatcgacgac tctatctaga
    21241 tgagacatca gaaatcaata ctaatttaag tcgactttct aaaaaaatgt ctcacctgac
    21301 tgctaacatg caaaagaaag aaagtgctta tattcttgat agccaagaag ttgtaaaaca
    21361 agagcgcaaa cgaattgcaa gagatttaca tgacacagtc agtcaagagc tattcgcttc
    21421 gtcattgatt ttatcaggaa tttcgatgag tttggaacaa ctggacaaaa cacaattaca
    21481 aacacagtta acaacggttg aagcaatgtt gcaaaatgct caaaatgatc tacgcattct
    21541 ccttttgcat cttagaccta ccgagctagc taatcggact ttatctgagg gccttcatat
    21601 gattcttaag gaattaacag ataaaagtga tattgaagtc atttataagg aaaccattgc
    21661 tcagcttcct aaaacaatgg aagataatct ttttagaatt gcccaagaat tcattagcaa
    21721 cacgttaaaa catgctaaag ctagtcgaat tgaagtttat ctcaatcaaa cctcaacaga
    21781 attacaattg aagatgattg atgatggtgt aggatttgat atggatcagg taagggattt
    21841 gagttatggt ctgaagaata ttgaagaccg tgtcaatgat ttagcaggaa acctacattt
    21901 aattagtcaa aaaggcaaag gagtttccat ggatattaga ctgccgatag tgaagggaga
    21961 tgacgatgag taagataaaa gtgatattgg tcgatgatca tgaaatggtc cgcatgggac
    22021 tcaagagttt tttgaatttt caagctgata ttgatgtcgt tggtgaggcc tctaatggac
    22081 gtgaaggggt tgatttagca ttggctttga agccagatgt tttggttatg gatctagtga
    22141 tgccagagtt aggcggtgtt gaggcaactt tagaagtcct aaaaaaatgg aaagaggcta
    22201 aggtacttgt gttaacttcc tatctagata atgaaaagat ataccctgtc attgatgcag
    22261 gagcgaaggg ttatatgtta aaaacatcga gtgcggctga aattttaaat gccattcgca
    22321 aggtttcaaa gggagagtta gccattgaaa cagaagttga caaaaaaatt aaggcgcatg
    22381 atcaacaccc tgacttgcat gaggaactaa cagcgcgtga gtatgatatt ttacaccttt
    22441 tagctaaagg gtatgataat cagaccatcg ctgatgaact ctttatttcc ttaaaaaccg
    22501 tcaaaacaca tgtgtccaat attctagcca agttagaagt tgatgaccga acccaagcgg
    22561 ttgtctatgc ttttcgacac catttagtcc cccaagacga taattaatgc tataatatag
    22621 agtatagcaa attggaggaa agtatggacg caaaactaaa atacaaagct aaaaaaatca
    22681 aaatggtgtt ctttgatatt gatgatacct tgagggtcaa agatacgggt tatatgcctg
    22741 agtcgattca aagagtattt aaggctttga aggcaaaagg catcttggtt ggtattgctt
    22801 caggtcgcgc tcggtacggt gttccacaag aagttcaaga tttacatgct gactattgtg
    22861 tcaaattaaa tggagcttat gtcaaagacg atgctaaaac tattattttc caagctccga
    22921 ttccagcaga tgttgttgtg gcctataaaa aatgggcgga tgatatgggc attttttatg
    22981 gaatggcggg tcgtcatgaa gcggtgttgt ccgctcgaaa tgacatgatt agcaatgcta
    23041 ttgataatgt ctacgctcaa ctagaggtct gtccggatta taatgagtat catgatgttt
    23101 accagatgtg gactttcgag gacaagggag atggcttgca gttgcctgct gaattagcag
    23161 agcaccttcg tttggtcaga tggcacgata actcatcaga tgttgtcttg aaaggtacgt
    23221 caaaagcgct aggtgtttca aaagtagttg accatttagg attaaaacct gaaaacattt
    23281 tggtttttgg agatgaactc aatgacctag agttatttga ctatgctggt atcagtattg
    23341 caatgggagt atcacatcca ttgctgcaag aaaaagcaga ttttatcaca aaaaaagttg
    23401 aagaagatgg cattttatat gccttggagg aattaggatt gattgacaaa gaattacagt
    23461 ttccacaatt ggatttgcca aaccataaag gaccaaaagc aaccattaaa acaaaccatg
    23521 gcgatatgac cctggtcttg ttcccagatc atgctcctaa aacagtagcc aatttcttag
    23581 gtttggctaa agaagggtac tatgatggga ttatcttcca ccgtattatt cctgaattta
    23641 tgattcaagg gggcgatcca acaggaacag gaatgggtgg acaatccatc tatggtgaaa
    23701 gttttgaaga tgaattttcg gatgaactct ataatctccg tggggccttg tcgatggcta
    23761 atgcgggacc aaatactaac ggcagtcagt tctttattgt tcaaaatagt aagattcctt
    23821 atgccaaaaa agaactagaa cgtggtggtt ggccggctcc aattgcagct tcttacgctg
    23881 caaatggtgg aactcctcac cttgatcgtc gtcatacggt ctttggtcag cttgtggacg
    23941 aaacttcatt ccaagttttg gatttgattg ctggcgttga aactggcgca caggataaac
    24001 ctaaagaaga cgttatcatt gaaacaatcg aggtatttga ctaatgaaaa ttggcgacaa
    24061 actgcatggc accatcactg ggattaaacc atacggagcc tttgttgccc ttgacaatgg
    24121 gacaacaggt ctcattcata tttcagaaat aaaaacggga tttattgacg atattgatca
    24181 attattggct ataggcaacc aggttttagt acaagtaatt gatattgatg aatatagtaa
    24241 aaagccaagt ttatctatgc ggaccttagc agaagaaaaa cagcattttt tccatcgtca
    24301 tcgttattcc aacagtcggc acaagattgg ctttaggcca cttgaagagc agttacctca
    24361 gtggattgaa gaaagcctgc agtttttaaa aaagaacact aaaaagagtc tggaacctta
    24421 gtagttccaa accctttttt atttcgtctt aattactggt acatcataga gttcagtcga
    24481 taaatagcgt tcgccattat ctggtaaaat agttaacaca tgtttgcctt ttcctaactg
    24541 tttagcgact tcaatagcgg cgtaaagagc agctccagaa gaaatcccaa ccaggaagcc
    24601 ttccttagct cctgttagtc gagcagtttc taaagcatct ttcgatttaa cacggataat
    24661 ttggtcatag gctttggtat ctaacgtgtt tgggataaat ccagctgata taccttgaat
    24721 tttatgtggt ccaggctctt gaccagataa gacagcagat tcttcagctt caacagcata
    24781 gataacagtt tcagggctag cttttttcaa gacatgtgaa acaccagaaa gtgttcctcc
    24841 agtaccaaca ccagatacga atgcatctaa agaaatctcc ttaaaagctt ccaaaatttc
    24901 ttgagctgtt gttttttcat ggatgcttgg attggcaggg ttattaaatt gcataggcat
    24961 ccaagcacct agttctattg ctaaagtttc agcttttgca atagcccctt tcataccttc
    25021 tgctccaggt gttaagacaa gctctgcacc ataagcctga atgatttgcc gtctttccaa
    25081 gctcatagtt tcgggcataa caataatgac tcgataccct ttagcagcac ctacccatgc
    25141 aagaccaata cctgtattac cacttgttgg ttcgataata acgtcaccag gacttatcat
    25201 accttcagct tcagcagctt caatcatcga taaagcaata cgatctttaa cagaagatcc
    25261 tgggttaaaa gcttctaatt ttacataaac gtcagcagct tcgtttggaa ttaaacggtt
    25321 aagtttgata ataggtgttt gatctactaa ttctgttata gttttgtaaa ttttagtcat
    25381 agtttagcct cttttcttga tacaaaataa atacgctgtg tatttaagta taaggagaga
    25441 tattattaaa gtaaaataca gatttactat cactctgata ataaagccat atcacttttg
    25501 ttgatgatta atcacagggg aattcaataa ctttggatcc gattttctca cagcttatct
    25561 gaccatgata ataatttgtt aaagtatcaa tgagattttc cacacgttca gtatcacaat
    25621 aaagtgttgt ctttatcgtg tctgaaaact tagtttctgt ttcggttaaa gccaattgat
    25681 ctagcaagtt agagtatagt tgatattgag gataagaaag tgtgatttct agtcctatct
    25741 gctgtttaac ttcaatgatg ccaaatcgtt taatagctgt tgcggtcatg ttagagtatg
    25801 ctctgattaa cccacctgtt cctaatttaa taccaccaaa gtaacgcgtt accacaacta
    25861 ctacatttgt taaacactgc ttttctaaca cagaaaggat aggtattcca gcagttcctg
    25921 aaggttcacc atcatcactt gagcgcttga tctgtctgtt gttaccgata atcatcgcaa
    25981 agcaggaatg attagctttg taatgttctt ttttgatagc attaacaaaa ttcttaccat
    26041 cttcttcagt gctcactcgt ttgatatgac aaatgaaacg agatttttta atactttctt
    26101 cgaaaaaacc actggctttg attgtcttaa aatgttccat aaaaaaattg tacaagaaaa
    26161 gataaaaatt agcaaaaaaa agcgaataat aaagtatgga gtctattgaa aatagttatg
    26221 gacggttgtt gctagaaagc cagttaccag attcagcaaa gcagcttgct caacctttaa
    26281 aaagtgtagt aatattgaga ggaaaaatga tttgtcaacg ttgtcactat cagcttgatg
    26341 aagaagcacg attgccaagt ggagcctatt attgccgatt ttgtcttgtc tttggacgaa
    26401 atcaatcaga taaattgctc tatgctatcc cacctatgca ttttccaaaa ggtaattatc
    26461 tggtttggag cggtcaacta actgcttatc aagagataat ttcccaacaa ttattaatca
    26521 atatgcaaaa tcaaaaaaca acattagttc atgctgtaac tggtgcagga aaaacagaaa
    26581 tgatttatgc tgcgattgag gcggtaatta acacaggtgg ctgggtttgc attgccagcc
    26641 caagagtaga tgtttgtgta gaggtggcta ctcgtttatc ccaagccttc tcctgcagca
    26701 tctgtttaat gcacgcagag tctcttcctt atcaaagagc tcctattata gtagccacaa
    26761 cgcatcagct gctaaaattt cacaaggctt ttgatctctt aattattgat gaagtagacg
    26821 cattcccttt tgtaaataat attcaacttc attatgctgc aagtcaggct ttaaaagaag
    26881 gtggcgcaaa gattttatta actgcaactt caactagaac tttagaaaga aaggtaaaca
    26941 agggagaagt agtcaagctg acattg
//
LOCUS       DAUCWO010000018        25526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUCWO010000018 DAUCWO010000000
VERSION     DAUCWO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.018"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            43..429
                     /locus_tag="VGY20_000707"
     CDS             43..429
                     /locus_tag="VGY20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ4273578.1"
                     /translation="MDEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLAL
                     REAVENNKISWKYINKILVDWYKSGDTTVEKVRDRLQRFDDSKKQRSVTTSNVPSWSN
                     PDYKEPDLKEFALGSMDGIEDGSGDF"
     gene            410..643
                     /locus_tag="VGY20_000708"
     CDS             410..643
                     /locus_tag="VGY20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273579.1"
                     /translation="MDQEIFNFFNKQIKKDFGKTASKETFAKFASYCAEGIEKNGVKP
                     IFNWINLYAFGTGMTTAEADRLRIERYKQENAL"
     gene            640..780
                     /locus_tag="VGY20_000709"
     CDS             640..780
                     /locus_tag="VGY20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273580.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKARYVTLLRQLRKKY
                     AI"
     gene            789..995
                     /locus_tag="VGY20_000710"
     CDS             789..995
                     /locus_tag="VGY20_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273581.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAIDDLGGVLTSREVFLAA
                     GITYLGAGQTDIHAAVEGLCEQIQ"
     gene            1051..1381
                     /locus_tag="VGY20_000711"
                     /pseudo
     CDS             1051..1381
                     /locus_tag="VGY20_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1384..2310
                     /locus_tag="VGY20_000712"
     CDS             1384..2310
                     /locus_tag="VGY20_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEQ4273582.1"
                     /translation="MANQLSTYTHKQFFNAPTVQKAFDDVWKGAGTQFAVSILSVLQG
                     SQSLKSASNKSIYAAAMKAAVLNLPIEPSLGRAYLVPYKGQAQFQLGYKGLIELAQRS
                     GQYKNINAGIVYKSQLISYNPLFEELILDFSKPQDEIVGYFAAFKLFNGFEKVSFWTV
                     EKVTAHGKKFSKSFASGPWKTDFDAMAQKTILKDILSKYGPLSVEMQKAIEEDNQDST
                     ISTPKDITPQEANSLDDLIGHQNENKDAPNNLKDVTEDLHDEEEKTLTDENKTVLEDT
                     SYPADEVPDFDQETGEVLASEGNLFDNLGDLI"
     gene            2307..2507
                     /locus_tag="VGY20_000713"
     CDS             2307..2507
                     /locus_tag="VGY20_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273583.1"
                     /translation="MIKLVKIEGYYINPEYVVGVWERSALNFDDLDEKVVVIQFVGDR
                     EREEFVFCDTPIDEVIKRLLDD"
     gene            2500..3295
                     /locus_tag="VGY20_000714"
                     /pseudo
     CDS             2500..3295
                     /locus_tag="VGY20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
     gene            3660..4055
                     /locus_tag="VGY20_000715"
     CDS             3660..4055
                     /locus_tag="VGY20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273584.1"
                     /translation="MDELKEKALSKMLDEMNKKHSAAEDAIHNWLSDQDDEELFKGIL
                     DDKKSIKDAMKYCINQAQKQKTGNCAMVDDKTVFGWVRVYFTGKTKKIEPVQATVTVS
                     QENTKVIPKKNKKSKGVVEGQLDLFGALV"
     gene            4052..5398
                     /locus_tag="VGY20_000716"
     CDS             4052..5398
                     /locus_tag="VGY20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HEQ4273585.1"
                     /translation="MKKNQKIIEGRLKPPKAFFDWCYEQIPTIRWQNKEKTIETEREH
                     KVYIDNRRLTKRTRLDFFDKFYSFAIVLVTSKRIEIQSYGFYSRYSNGRQSIEVKLCN
                     FEKLANNHHIKLTEQFGQYVPGLTPNFIGSGAYSGTKFYPDDWEEKIKSISELKYLQF
                     PIGLAHHNLPHMYKYRTQIEFLQKIDAWKIANDLAYDVTEYDAWHVRKAVDCRVITKK
                     WLHQNKQFFKNSNRSFREYELERRIKARNGKLVPGIEKILSYQDINKIPKAAKINRFQ
                     NWLVKNKVDFNYYIDYLSMLDELGIEIDTDNLIMPKDLVKAHDNVVKLVNQKKSEIEK
                     RKFKNRLKSLSKYEQTIGDYCFRAPVDSGELIREGKTLSHCVGSARYTQAHASGKTTI
                     IFIRRKSDSDKPFYTMEYKAGHIVQVRGKHNQSATEEVQKVVDQWLAIVNKNYKHA"
     gene            5409..5741
                     /locus_tag="VGY20_000717"
     CDS             5409..5741
                     /locus_tag="VGY20_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273586.1"
                     /translation="MITKINVPKTSIVIEIENKEITIENLINYDIKMIFRNQDNEPSL
                     DENGDIFEPMYWLDIKAKPNDEIEYHSSLGVKKEKRRLAELQIFFEYIEANKQNLFDL
                     CGLKGELA"
     gene            5738..6250
                     /locus_tag="VGY20_000718"
     CDS             5738..6250
                     /locus_tag="VGY20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273587.1"
                     /translation="MSSLTLSLDVSTTGTGWAIFDGSTLIQSGASKPKQKSFYERAKS
                     MASELKTIQLRAIQKHDKPFEDIVIEQNTVLGPNQQSSIKIGIATGIILGRLLAEEIY
                     FVNVSTWRKYWKFSYKDRSKKSMKKQAINAVAAEFNKQVKDDEADAILIGSYFTNLGK
                     DFGDLESHKN"
     gene            6286..6603
                     /locus_tag="VGY20_000719"
     CDS             6286..6603
                     /locus_tag="VGY20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEQ4273588.1"
                     /translation="MEIVKDGVKFIASMIIVLAATFGCGYLFYRAGYEQAKKEVQPVT
                     IYTVDNVGGVMVGEITDKEIIEGRYTVTAHAYGKFLVTKEQYKAIKVGDKIPDYLKKR
                     GTK"
     gene            6600..6755
                     /locus_tag="VGY20_000720"
     CDS             6600..6755
                     /locus_tag="VGY20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273589.1"
                     /translation="MIELTQKQADYVEFVRKTIVDPDMMEFAVNTQLISYALLVGYCI
                     VDEEVAK"
     gene            6752..7003
                     /locus_tag="VGY20_000721"
     CDS             6752..7003
                     /locus_tag="VGY20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273590.1"
                     /translation="MKPRKYPYSGKIRIIKKELPRFVRLGDFAFNSNLVKHIDKIRQV
                     KPNETLIRFKIPKLFMTYEEETFKVRLEIDKVVKILNQY"
     gene            7079..7498
                     /locus_tag="VGY20_000722"
     CDS             7079..7498
                     /locus_tag="VGY20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ4273591.1"
                     /translation="MSKAKAILKDLRNLDLYIASLIRRQEKIETSLLSSPKWSSDKVN
                     GGIKRKQDDVYVELIATAEDIEKKTAEAIRKQRELQNLIDSLENTDSQTILSMVYIDK
                     MTRWQVIDELNCSESTYFRLLRVATKELNNLTVNDSD"
     gene            7606..7950
                     /locus_tag="VGY20_000723"
     CDS             7606..7950
                     /locus_tag="VGY20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ4273592.1"
                     /translation="MSQLRADKKGTHRVAFEKNKRKLLKTTNLCGICGKPVDKTLKYP
                     HPLSAAIDHIVPIAKGGHPSALENLQLTHWQCNRQKSDKLFANQASNEPKTIGNRNLP
                     QSRDWSSFTLKK"
     gene            8098..8454
                     /locus_tag="VGY20_000724"
     CDS             8098..8454
                     /locus_tag="VGY20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273593.1"
                     /translation="MKKKCLICKKNFQAKTNRTLYCSEECRKKGNREKQRKLMKQKRA
                     EQRKEKKKVLNPNTDVTEKPKKIRNLAQHYKKLKKEILANESEFGFTGITLIEGIDVH
                     EENFVDLVMQKIKEQK"
     gene            8451..9719
                     /locus_tag="VGY20_000725"
     CDS             8451..9719
                     /locus_tag="VGY20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ4273594.1"
                     /translation="MNYMGMGYLRRKLALFKTGVDKRYRYYAMDDRDDTRSIVMPNNV
                     REMYRSVLEWTAKGVDSLADRIIFREFTNDDFNAWEIFKANNPDIFFDTAIQSALIAS
                     CCFVYIMPGAEDGLPKMQVIEASKATGILDPTTFLLTEGYAILESDSNGNPTLEAYFT
                     DKDIWYYPKKGKPYNIKNPTGHPLLVPIIHRPDAVRPFGRSRITKAGMYHQKAAKRTL
                     ERAEVTAEFYSFPQKYVLGMDPDAEPMEKWRATVSTLLEISKDEDGDKPTVGQFTTAS
                     MAPFMEHLKMYASLFAGGSGLTLDDLGFPSDNPSSVESIKAAHENLRAAGRKAQRSFS
                     SGFLNVAYIAVCLRDEFPYLRNQFMDTVIKWEPLFEADANMLTLVGDGAIKLNQAIPG
                     FMDADVIRDLTGVKGADKPIPAITEVTTDG"
     gene            9712..11205
                     /locus_tag="VGY20_000726"
     CDS             9712..11205
                     /locus_tag="VGY20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273595.1"
                     /translation="MVDDVLPKLLKSVRQDFEKYFGESDVVTKAFAELQAKKVTYKTV
                     NEFAIEVGRLLSLALTGSVSSDKLPDGKMYYNIAKRLLDETMGRNYKLISGYAGDVQR
                     ILNENAQIGLKVQRPPLNRDKINGMVNRLDSENTFDDVKWLFGEPIVNFSQSIVDDTI
                     KANADLQYKTGMTPQVVRTESGNCCEWCREVVGTYSYPKVPKDVWRRHQRCRCTLDYD
                     PKNGKVQSAWSKIWRKKEKTQESIERVEKFKESALVESIKNDIAKLDMTKVGPSDIID
                     IGKRINYHFRVSEHIGDKEKLKEIFSNFREIGGEIPKNTWAKGSSKLVKDQLQEAFQN
                     YPTEWAAVPDGIGKKLKAIKRKRGYFDGYDEDLVIATNGTRKTTPYHEIGHMIELVNP
                     DLVRLEKAWVDKRTANEAEVRLKDIFPSSNYGIGEVTKKDDFISPYIGKYYSDAAEVF
                     TMGLQGIFVPEERFAKSFDKKTWKYDYKTINDDPEFLNFIIGLFVKV"
     gene            11211..11435
                     /locus_tag="VGY20_000727"
     CDS             11211..11435
                     /locus_tag="VGY20_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273596.1"
                     /translation="MEPKLHRQLRQKYDDAEKQYLEKFGEDSLDRVFFWEPDVYFDEW
                     KKVLPDATLELNKAINSGVAIDPDPENAIY"
     gene            11512..11664
                     /locus_tag="VGY20_000728"
     CDS             11512..11664
                     /locus_tag="VGY20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273597.1"
                     /translation="MTWENKDEFQQIMNKVNETKEAYEKALEAAENFVPKMSCATKLN
                     KGEGNE"
     gene            11657..11923
                     /locus_tag="VGY20_000729"
     CDS             11657..11923
                     /locus_tag="VGY20_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273598.1"
                     /translation="MNKRIKKKRKLETAIMLLIAENALQAEAIKNQNKEIMELKSIVQ
                     RNALATNEELATVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            11925..12140
                     /locus_tag="VGY20_000730"
     CDS             11925..12140
                     /locus_tag="VGY20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273599.1"
                     /translation="MKYRKKPVVVEAIQFTGTNYEKIKEFIGQNTLCSTLSIVIPTLE
                     GDYIIKGIAGEFYPCKPDVFEQTYEST"
     gene            12222..13637
                     /locus_tag="VGY20_000731"
     CDS             12222..13637
                     /locus_tag="VGY20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ4273600.1"
                     /translation="MVTKTKTKLGNQRPTQSVNLHFAKSLAHEAINYYKKTGLSCYPW
                     QVNMLIPIMAIDENGLWVHQKYGYAIPRRNGKTEVVYIVELWALHKGLKILHTAHRIS
                     TSHASFEKVKKYLEMSGYVDGEDFISNKAKGQERIEFKASGAVIQFRTRTSNGGLGEG
                     FDLLIIDEAQEYTSEQESALKYTVTDSDNPMTIMCGTPPTMVSTGTVFEAYRKDCLKG
                     NKRYSGWAEWSVPEMVKINDVSSWYISNPSMGFHLNERKIEAELGEDEIDHNIQRLGY
                     WPSFNQKSVISEKEWAKLKVEQVPELKSKLFVGIKFGQDGNNVSLSIAARTSENKVFV
                     ETIDCLSVRNGTQWIINFLKSADIAKVVIDGASGQELLAQEMKEQGLKKPELPKVAEI
                     ITANMMWEQGIMQETICHSDQPSLTAVVTNCEKRQIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWICYTTKPKRKQRTSC"
     gene            13718..14179
                     /locus_tag="VGY20_000732"
     CDS             13718..14179
                     /locus_tag="VGY20_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ4273601.1"
                     /translation="MSEFKVIETQEELDTIVKARIAREREKYQDYDQLKTRVEELETE
                     NSSLQTALNDAKSNTDSYTEEISTLKNQIADYETANLRTKVALQYGLPIDLADRLQGD
                     DEDGLKVDAERLASFIKPSQPQPPAKSNEPNIDSNADANYRALVQGLSTED"
     gene            14204..15115
                     /locus_tag="VGY20_000733"
     CDS             14204..15115
                     /locus_tag="VGY20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ4273602.1"
                     /translation="MGTETSKASLFDKHLVSDLINKVKGHSSLAKLSSQKPIPFNGSK
                     EFTFTLDSDIDVVAENGKKTHGGLSLEPVTIVPIKVEYGARLSDEFLYATEEEKIDIL
                     KAFNEGFAKKLARGIDLMAMHGINPRTKKASDVIGTNHFDSKVTQVVKFTESEDADAN
                     IEAAVNLIQGAEGVVTGLAMDTEFSTALAKVTNGEMGPKMYPELAWGANPDSINGLKS
                     SVNTTVGAGADEAESKDLVIIGDFESMFKWGYAKQIPMEIIKYGDPDNSGKDLKGYNQ
                     IYLRAEAYIGWGILDAKSFARVTKGEV"
     gene            15115..15315
                     /locus_tag="VGY20_000734"
     CDS             15115..15315
                     /locus_tag="VGY20_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273603.1"
                     /translation="MLYVNSKTGASFYSSVPVCGGDWTLVEQQTGEKAKTVAEIKRQL
                     DAAGIKYSSKAKKPELEALLPG"
     gene            15325..15747
                     /locus_tag="VGY20_000735"
     CDS             15325..15747
                     /locus_tag="VGY20_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HEQ4273604.1"
                     /translation="MGNFATTDDVILLWRPLSVDELKRANALLKVVSDTLRMEADKVG
                     KDLDKTMVDKPYFVNVIKSVTVDIVARTLMTSTQGEPMSQESQSALGYTWSGTYLVPG
                     GGLFIKDNELKRLGLKKQRYGGIELYGEIKRDNDYFDR"
     gene            15707..16045
                     /locus_tag="VGY20_000736"
     CDS             15707..16045
                     /locus_tag="VGY20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273605.1"
                     /translation="MGKLRGITITLIDKVTIDIDPFGNPIKKDKEISVDNVLVSPATS
                     DDITSQLSLSGKKAVYTLAIPKGDNHDWGDKEVRFFGEKWRTVGLALEGIEELIPLEW
                     NKKVMVERYE"
     gene            16038..16274
                     /locus_tag="VGY20_000737"
     CDS             16038..16274
                     /locus_tag="VGY20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273606.1"
                     /translation="MSKFKFKLNKAGVAELMKSSEMQQVLTTKATAIRERCGDGYAQD
                     IHVGKNRANAMVSAKTIKAKKDNSKNNTLLKAVR"
     gene            16275..16610
                     /locus_tag="VGY20_000738"
     CDS             16275..16610
                     /locus_tag="VGY20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273607.1"
                     /translation="MIEVIIKKYLDEHLDVPSFFEHQKDEPARFIILEKTSGAKQNHL
                     LSSTFAFQSYAESLYEAALLNDKVKQVIEQLDVLPQVSGVHLNADYNFTDTATKRYRY
                     QAVFDINHY"
     gene            16626..17216
                     /locus_tag="VGY20_000739"
     CDS             16626..17216
                     /locus_tag="VGY20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4273608.1"
                     /translation="MANDTKNVTSAKPKAGGAIYSAPLGTTLPEDAKSKLDTKFKNLG
                     YVSEDGVVNEDTRSSENIKAWGGDIVGSVQTEKEDKFTYKLIESLNVEVLKEVYGAAN
                     VTGDLDKGIHIKSNSKELEAHAIVIDMIMNGGILKRIVLPNAKVDEVGEIKYVDGEVV
                     GYETTLKCFPDEKGDTHHEYIVKPGEANKKENSFEM"
     gene            17227..17490
                     /locus_tag="VGY20_000740"
     CDS             17227..17490
                     /locus_tag="VGY20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273609.1"
                     /translation="MEILKGKTTSGFEYEIPKKRLKNFELVEAIAEEETDPTAVVKIV
                     NLLLGDAAKSLKEHVRDAEGIVDVEAIGVEIKEIFESQKDLKN"
     gene            17505..17876
                     /locus_tag="VGY20_000741"
     CDS             17505..17876
                     /locus_tag="VGY20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HEQ4273610.1"
                     /translation="MIAKDDDALTCDLAETYGIYDYRQLPAYQVAVFAVGLRSNSRIK
                     MALSGETEALDTVLLAGIYDNTNLLFWSKTKDGQSGQNKPKSVVEAISGSKSQKANDV
                     ISFASGEDFENARKQLLGGDG"
     gene            17876..20239
                     /locus_tag="VGY20_000742"
     CDS             17876..20239
                     /locus_tag="VGY20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273611.1"
                     /translation="MATELGQAYVQIMPSARGISGAISKQLDPEARSAGLSAGKLFGS
                     KLKLAALAATAGLGILLKKTISASLSTGADLQQSFGGIDTLYKGAETAVKGFAKEAYK
                     AGISANTYAEQAVSMGASLKQSLGGDAVAAAKAANMAIMDMADNSAKMGTDITSIQMA
                     YQGFAKQNYTMLDNLRLGYGGTKEEMKRLLSDAEKLPAAMGKKFDLSNYADVVEAIHL
                     VQDNMGIAGVAAEEAKTTFSGSLAAMKSSFTNVLAGLSLGDDIRPALRGLAETTSNFL
                     FGNFIPMVANIFKGLPSAIGTFIGAATPIIASQFKGLMSSLGISIDLSPITAKFAQIG
                     QNLQPVFNGLKTAFSQLPSFFTSIGSAVAPVIDTIISGLARLDFSGFEALISAILPAI
                     QTGFQTFMSIVGPAISQLVNSFVNLWNACQPLVTILSGALMPAFQILGAFLGGVLQGI
                     LGSISFAFDALKVVIEFLTPIIDWLVQGFNAISPALQIVAQWVGNVIGIFTSLGASGQ
                     GLSSMLSSAWGNIQTVVSTAKNMITLAIDGIKLVFSNLGNAGNILKGLLSAAWSAMQN
                     AVVIAKGIINSAISAIKTAFSSFGNLVSSVSGTIKSVIGSLKNAFYSLASIDLVGAGR
                     AIMQGFLNGLKSMWGAITGFVGGIADWIRKHKGPISYDRVLLKPAGKVIMQGFNNSLM
                     DGFKEVKSNVSGMADDLAGTITGKSLSLGIDAKPSVTADDLLSSNISTKTTVGSATSD
                     LSLFFVKVLALLQDILDKNTDVYLDKEKVSAILYEEFAKIMAREGIA"
     gene            20236..20931
                     /locus_tag="VGY20_000743"
     CDS             20236..20931
                     /locus_tag="VGY20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273612.1"
                     /translation="MIPKVIIDGFDTSTIPNCVLTGYDVGDVLSPHFVENEVYGMNGT
                     SRGLESYNESKPTLTWHLSTFDDAVNLVNHLDGLGKIIEFWHIPKSFYYYDCLSVKIN
                     AVTMNSWRVTLKLALYPFRYAKGISDVTIAGNGSINNPGNVFSEPKIIVEGTGKGTLT
                     IDKQVMELNLSGKATIECKHGQQCVYDGEGNVKNSIRIRGRFFEIQPGTQGIAVSGGI
                     ARLTISPRWRYKV"
     gene            20913..22886
                     /locus_tag="VGY20_000744"
     CDS             20913..22886
                     /locus_tag="VGY20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4273613.1"
                     /translation="MEVQGLISIKDDNTPLVAAFEDEITQEANSDYKLSFKYPAKHEY
                     RPLIKKGIILEADDLHGTQLFRVFEITKRHGYINVYGNQVADDLNGYAIDSISVDRVQ
                     GMTVMSELAGNIKREHPFSFFSDIDGRHTFNQSDVSVMDALANGKHSIMGQWGGELVR
                     DKYQVNLLKKAGKDTETLFMYKKNLKSYEETDTIKGLISILHLVAEVEEQREEESREV
                     SDADIGHNEVSKKKTIRVTVESKLKDTHPIIVEKTIKVQDQDVKTEEDLLAYGKKYFE
                     KTLCDIPGNSLKIDVTNNYEGAVRLFDTAIVFHELYDRDLRMQITGYRFAPMANRLKS
                     IIFGEIKTNLAKQISNQIDNKVAESTAQHDAAFEAKLQKQIDNANRIFDTKEAKLREE
                     IEDGIKKAEANAEVKVAEVNAKVLEAEELAKAVDERLKKFLSDADTKEQDFDKKLEEF
                     RTSLKDLEVDEKQIDDALAKAGFSKDSLADIKAKLEDTSETATVTANIVGSTGGTFYN
                     RNRLDGDTDKVITFEQGYIDIAHNGEGFEEGKTYTISFEATCELLRKVGITVTQANMK
                     GARLVLTPKNPKLVVESFGLTKDTETINVYPFSYTVLVTSDWYKSKQIDLNASEVQEL
                     ALEMAYKDVVDGNNATIAGQWSDSPQMILDGGS"
     gene            22886..23995
                     /locus_tag="VGY20_000745"
     CDS             22886..23995
                     /locus_tag="VGY20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115223326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ4273614.1"
                     /translation="MTENIPLRVQFKRMSADEWARSDVILLEGEIGFETDTGYAKFGN
                     GKSKFSALKYLTGPKGPKGDTGFQGKTGGTGPRGPAGKPGTTDYNQLQNKPNLDAFAR
                     KQETDSKITELKSNKADKNAVYLKAESNAKLDEKLSLTGGIVTGQLQFKPNSGIKPSS
                     SVGGAINIDMSKSEGAAMVMYTNKDTTDGPLMILRSDKDTFDQSAQFVDYSGKTNAVN
                     IVMRQPSAPNFSSALNITSANEGGSAMQIRGVEKALGTLKITHENPNVKANYDENAAA
                     LSIDIVKKTNGEGTAAQGIYINSSTGTTGKMLRIRNKNEDKFYVGPDGGFHSGANSTV
                     AGNLTVKDPTSGKHAATKDYVDEKIAELKKLILKK"
     gene            24010..>25526
                     /locus_tag="VGY20_000746"
     CDS             24010..>25526
                     /locus_tag="VGY20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273615.1"
                     /translation="MSRDPTYTINEHDLSFADGRFYVTFKADKSSETVRLNSSCLGNT
                     IIKKLQVEDDNTMHDFVKPKVTTQQAFGLAQQVKELDLQLKDPKSDLWGKIKFNNKAM
                     LVEYANKEMSSAIAQSAEQILLQVKSIDDERYSKFEQTLNGIKQTVKSESVESARTQL
                     ASMFDSRISGLDGKYSRLSQTIDSLSSRLDDGVGNYSTLSQKVSGIDLRVSNAANDVS
                     RLSQTAQGLQSQITNANQNYSSLSQTVQGLQTTVRDNQSNATSRINQLSDLISTKVSK
                     GDVETTIAQSYDKIAFAIRDKLPASKMSGSEIISAINLDRSGVKITGKNITLDGNSYI
                     SNAVIKDAHIANMDAGKINTGYLNANRIATEAITGEKIKMDYAFFNKLTANEGYFRTL
                     FAKDIFATSVQSVTLSASKITGGVLAATNGASQWDLNNANMTFNRDATINFNSKNNAL
                     VRKDGTHTAFVHFSNATPKGYRGSALYASIGITSSGDGIDSASSGRFCGVRFFRYA"
BASE COUNT         8889 a         4211 c         5603 g         6823 t
ORIGIN      
        1 actaacagat aataagcatc atcatcaaca tcaggaggag ctatggacga aaaaaagctt
       61 tttgaaaatt tccaattaac ttttggacgg atgatatcgc catttgaaat cgaagatatt
      121 caaaagtgga ttcacgaaga taacatgcca attgaagttg tcaaccttgc cttaagagaa
      181 gcggtagaaa acaacaaaat cagttggaag tatatcaata aaatcttagt tgattggtat
      241 aaatctggag atacgacagt agaaaaggtc agagacaggt tgcaacggtt tgacgatagt
      301 aaaaaacaac gaagtgtaac tacctcaaac gtcccaagct ggtcgaatcc agactacaaa
      361 gaaccagatt taaaagaatt tgctctagga agcatggacg gtatagaaga tggatcagga
      421 gatttttaat ttttttaaca aacaaatcaa aaaagatttt ggtaaaacgg cgagtaaaga
      481 gacttttgct aagtttgcta gttactgcgc cgaaggaatc gaaaaaaatg gagttaagcc
      541 aatttttaat tggataaacc tatacgcttt tggaactggt atgacaacag cagaagcaga
      601 ccggttgaga atagagcgat ataaacagga gaatgcgtta tgacaaaaca acatagagaa
      661 acgcttatct ggtaccgagc aagtcatcaa gagcgtgaga gattgcttga ttttggacta
      721 gttgataaag cacggtacgt gacactattg cggcaattgc gtaagaaata tgcgatttag
      781 gaggaaatat gacaccagaa caagcagaaa aagcaaaaat cagagctaaa caagaacttg
      841 aaacgtttag catatacctt gatcaggcaa ttgatgatct cggtggagtt ctaacttcac
      901 gagaagtctt tttagcagcg ggaataacat atcttggcgc aggtcagaca gatatacatg
      961 ctgcagtcga gggattatgt gagcaaatcc aatgatttta aatttagtga agactgggag
     1021 agcaactaaa acacaaaaag aggaaatgaa atgaacatca aagaaaaaat tgtagtgcta
     1081 agaaacactg aagacggaag ttttttaaag agtttcaaaa acaaaaaaga tgtacttgct
     1141 tataatgtgg aacttacaga tagcattcaa ctggcatcat ttttaccaga agaagcttac
     1201 aacatacaaa aagaaaaaaa ttgataattt ggcagaaacg cttgggtgtg atgttgtagt
     1261 tatcgaagca tcatatgacc taaaatttat tgatggcgaa tcagtgccag agttaacaaa
     1321 agagcaaaaa gttaaaagta tggtaaacgg aatgtttgag caggtttttg gaggtgaata
     1381 gagatggcaa atcaattatc aacatacaca cacaaacaat tttttaacgc accaacagtt
     1441 caaaaagctt ttgatgacgt ttggaaaggc gccgggacac agttcgcggt aagcatcttg
     1501 tcagtactac aaggtagtca aagtttgaaa tcggcatcta ataaatctat ttatgcagca
     1561 gctatgaaag cagccgtgtt aaatctgcct attgagccaa gcttaggaag ggcctattta
     1621 gttccgtaca aaggtcaagc acaattccaa ttaggctata aagggttgat tgagttagcg
     1681 cagcgcagcg gacaatacaa aaatattaat gcaggtatcg tctataaatc acaattaatt
     1741 tcttacaacc ctttgttcga ggaattaatc cttgacttta gcaagccaca agatgaaatt
     1801 gtagggtatt ttgccgcttt taaacttttc aatggattcg agaaagtttc tttttggaca
     1861 gtagaaaaag taactgcaca tggaaagaaa ttttcaaaat cgtttgctag cggtccttgg
     1921 aaaacagact ttgatgcaat ggctcaaaaa actattttaa aagatatttt gagtaaatat
     1981 ggtccgttat cagttgagat gcagaaagct atcgaggaag ataatcaaga ttcaacgatt
     2041 tctaccccaa aagacattac cccacaagaa gcaaatagcc ttgacgacct aattggtcac
     2101 cagaacgaaa ataaggatgc tcctaacaat ttaaaagacg taactgaaga tttacatgac
     2161 gaagaagaaa aaacgctcac agacgaaaat aagacggttt tagaagatac gtcttatcca
     2221 gcagatgagg ttcccgattt tgaccaagag actggtgaag tattagctag cgaaggcaac
     2281 ttatttgata acctcggaga cttaatatga ttaaacttgt aaaaattgaa ggatattata
     2341 ttaatccaga atatgttgtt ggcgtttggg aaagatctgc tttaaatttt gacgatttag
     2401 atgaaaaagt agtggttatt caattcgttg gagataggga aagggaagag tttgtctttt
     2461 gcgatacacc tattgatgaa gtaattaaga ggttgctaga tgactagttt agacttgctc
     2521 ggaaaggact attacagccg tgaatcagct atcaggtact ggtccattag tcagtacaag
     2581 cgttttaaag agtgcgaagc gcgggcgctt gctgaattac aagggaattg gacagatacc
     2641 agagataaca ctgcgttgct cgtcgggaac tatgtccact cttactttga gagtaaaaaa
     2701 gctcatgaag aattcaaagc ccaaaacggc tctgaaatga tttcgacccg aggagcaaca
     2761 aaaggccaat taaaaaagga ttacttagtt gctgaacaga tgattgacgc tcttaaaagt
     2821 gatagtaact ttatggctat ctaccaagga gaaaaggaag cagcaatcac aggatttctt
     2881 ggcgagattg aattcaaggg taaaatcgac tgcctgaatg ttgaacgtgg ctattttgta
     2941 gacatcaaaa caacaaaagg gccgattgac gacacgattt ggagtggcga agagcgtgtc
     3001 agatggtttg aggcttacgg atatatcttg cagatggctg cttacaaaac catgttagaa
     3061 gctaaatata ataaaccgtt cgagccgatt atttacgcgg taaccaagga aacgccacca
     3121 gatacaagag ccatcagaat ccaaaatgta gacgctatgc aaaacgagtt agatagccta
     3181 gcacaaagca ttaagcgatt agatgacgtt aaaaaaggca tagaaaaacc taagccttgc
     3241 ggtcattgag tattgcaggg ctaatcaatt aacacaaaga gtaatgattt tttaggagat
     3301 aaaataatga taatagagag gaataagcgg aggatatggt aagaagtgag tatgctagag
     3361 ctaaccacat ctctaaagaa tgggtagtac tttactagaa aagtggagga atttaaatga
     3421 ttgaattcgt aaaagaagta ggaatggctt tgttatggct gtttgtagga tatcttgttg
     3481 gagagcgtag ccaacaaaaa aataagtaaa aaatacgtgc cgtgaaccac gaaaaaagcg
     3541 aactagaaat aagtgtcatt taacggtcat gtgactagaa tgatgtaaag aatagcgact
     3601 gcccgtattt agccaaatta acaacttttg gcagtcgctt ttagttttag ggaaatgcta
     3661 tggatgaatt aaaagagaaa gcattatcca aaatgctaga tgaaatgaac aaaaaacata
     3721 gtgcggctga agacgccata cataattggc tatctgatca ggatgacgaa gaactattca
     3781 aaggcatact tgacgataag aaatcaatta aagatgccat gaagtactgc atcaatcagg
     3841 cacaaaaaca aaaaacaggt aattgcgcca tggttgacga taaaacagta ttcggttggg
     3901 tccgtgtgta tttcactgga aaaaccaaaa agatcgaacc agttcaagca actgtcacag
     3961 ttagccaaga aaatacgaaa gttataccaa agaaaaataa aaaatcaaaa ggtgtggttg
     4021 agggccaact tgacctattt ggtgcgcttg tatgaagaaa aaccaaaaga ttatcgaagg
     4081 tcgtttaaag ccacctaagg cattctttga ctggtgctat gaacaaatac ccaccatcag
     4141 atggcaaaac aaggaaaaga caatcgaaac tgaacgtgaa cacaaagtct atattgataa
     4201 taggcgcttg actaagcgga ctagactaga tttctttgat aagttttaca gctttgctat
     4261 tgtccttgtc acttccaagc gaattgaaat tcaatcctat ggcttttact ccagatatag
     4321 caatggaaga caatcgattg aagtgaagtt gtgtaacttt gaaaaattgg ccaacaatca
     4381 tcacatcaaa ttgactgaac aatttggcca gtatgtgcct ggattaacac caaattttat
     4441 aggctctgga gcctattcag gtaccaaatt ttatcctgat gattgggaag aaaaaatcaa
     4501 gtcaatatca gaactgaagt atttgcaatt tccgatcggc ttagcacatc ataacttgcc
     4561 ccacatgtat aagtacagaa cgcaaataga gttcctacaa aaaatcgatg cttggaagat
     4621 agcaaatgat ttggcttatg atgtgactga atacgatgct tggcatgtac ggaaagccgt
     4681 agattgtcga gtgattacca aaaaatggtt acaccagaac aagcaattct tcaaaaacag
     4741 taacaggagt tttagagaat atgagcttga gcgtcgaatt aaagctagaa atgggaagtt
     4801 ggttcctggt attgaaaaaa tattgtctta tcaagacatc aataaaattc caaaagcggc
     4861 caaaatcaac aggtttcaaa actggttggt taagaataag gtagatttta actattatat
     4921 cgattatctg agtatgcttg atgaattagg tattgaaatt gacactgata atctgattat
     4981 gccaaaagac ctagtaaagg cccatgataa tgttgtcaaa ttagtcaatc aaaagaaaag
     5041 cgagattgaa aaacgcaaat ttaaaaatcg tctcaaaagc ctttctaaat atgagcaaac
     5101 gattggcgat tactgtttca gagcacctgt cgattcagga gaactcatcc gagaggggaa
     5161 aactctatct cactgcgtag gaagcgcacg atatacacag gcccacgctt ctggaaaaac
     5221 aactattatt tttatccgga gaaaatctga ttcagacaaa ccattttaca caatggagta
     5281 taaagccggc catatcgttc aagtcagagg caaacataat cagtcagcta ctgaagaagt
     5341 ccaaaaagta gttgatcagt ggctggccat tgtaaacaaa aattataaac acgcatagga
     5401 gaaataacat gataacaaaa attaatgttc caaaaacatc catcgtaatt gaaattgaaa
     5461 acaaggaaat cacgattgag aatttgatta attatgatat taaaatgatt ttcagaaatc
     5521 aggataatga accgtctctt gatgaaaacg gggacatctt tgaaccgatg tactggctag
     5581 acataaaagc taaacctaat gatgagattg agtatcacag cagcttaggc gtcaaaaaag
     5641 aaaagagaag actcgctgaa ctacaaatct tctttgaata tattgaagca aataaacaaa
     5701 atctctttga cctctgtggg ctaaaaggag aactcgcatg agtagcttga cattatcact
     5761 tgatgtctct actactggaa cgggttgggc tatttttgat ggttcaactc taatccagag
     5821 cggggctagt aaaccaaaac aaaaatcatt ctatgagcgt gccaaatcaa tggctagcga
     5881 gctaaaaact atccaactta gagcgattca gaaacatgat aagccttttg aggacattgt
     5941 gattgaacag aacactgtac ttggaccgaa tcaacagtcg tctataaaaa tcgggattgc
     6001 aactggtatt atcttaggga gattacttgc tgaagagatt tattttgtaa atgtatcgac
     6061 gtggagaaaa tactggaaat ttagctataa agaccgctcg aaaaaatcca tgaagaaaca
     6121 ggcaatcaat gcagtagctg cggaatttaa taagcaagtg aaggatgacg aagctgacgc
     6181 gatactgatt gggtcttact ttactaatct tggaaaggat tttggcgact tagaaagtca
     6241 caaaaattga gagaataaag tgacatataa agtggaggta atgaaatgga aattgtaaaa
     6301 gatggagtta agtttattgc cagtatgata attgttctgg cagctacatt tggctgtgga
     6361 tatctgtttt accgtgcagg atacgaacag gcgaaaaagg aagttcaacc agttaccatc
     6421 tataccgtcg ataatgtagg tggtgtaatg gtaggagaaa ttacggataa agaaattata
     6481 gagggacggt acacggtcac ggctcatgcc tacggtaagt ttctagtaac aaaagaacag
     6541 tacaaggcga taaaagttgg cgataaaata cctgactatt tgaagaaaag aggtacaaaa
     6601 tgattgagtt aacacaaaaa caggctgatt atgttgagtt tgtcagaaaa actattgtag
     6661 atccagatat gatggaattc gcggtaaata cacaactgat tagctatgct ttacttgttg
     6721 gatattgcat tgttgatgaa gaggtggcca aatgaaacct agaaagtatc cgtattcagg
     6781 aaagataaga attataaaaa aagaattacc aaggtttgta agattgggag attttgcttt
     6841 taatagcaac ctagttaaac atattgataa aatcagacaa gtaaaaccaa atgaaacgct
     6901 aattcgtttc aaaatcccta agttgtttat gacatatgaa gaagaaactt ttaaagtaag
     6961 acttgagatt gataaagtcg taaaaatatt gaaccaatac taaacaaaaa aagccagctc
     7021 acagctgacc attggttaat aattcgataa atctattata ccaaaaagga gttaggaagt
     7081 gagcaaagct aaggcaattt taaaagactt acgcaattta gatttatata tcgctagttt
     7141 aattagacgt caggaaaaga ttgagacctc attgctttct agccctaaat ggtcgtccga
     7201 taaggttaat ggtggtataa aacgtaagca agatgatgtt tacgtagagt tgattgcaac
     7261 tgccgaagat atcgagaaga agactgccga agctataaga aaacaaagag agcttcagaa
     7321 cctgattgat agccttgaaa atacagacag tcaaacaatt ttaagcatgg tctatattga
     7381 taagatgact agatggcaag tgattgatga gctaaattgc agcgaaagca cctatttcag
     7441 actgttaaga gttgcaacta aggaattaaa taatttgaca gtaaatgaca gcgattgaca
     7501 gtgaataaca gtgcatgaca gttttaaagt gataatatag tattatcaag aaatgaggga
     7561 caggtaagaa aatacccccc tcttttaaat ttagaaaggc cccctatgtc tcaattaagg
     7621 gcagataaaa aaggtactca cagggtagca tttgaaaaga ataagcgaaa gctgttaaag
     7681 actaccaacc tctgtggtat ctgtggtaag ccagtcgata agacactgaa gtatcctcac
     7741 ccgcttagcg cagcaataga tcacatagtt cctattgcaa aaggtggaca tccgtcagcg
     7801 ctagagaact tacaacttac acattggcaa tgtaataggc agaagtctga caagttgttc
     7861 gctaaccaag caagcaacga gccaaagaca ataggaaata gaaaccttcc tcaaagtcga
     7921 gactggtcat cttttacatt aaaaaagtga atttgtgcaa attattgatt tatgctaaaa
     7981 ctggtcagaa gctaaacggg gggtatggcc ctccccggtc ggccggccga gcttcacgcc
     8041 gtcactgtac attttttctc acgttaggat ttagaaaatt ttggaggttg aatttcattg
     8101 aaaaagaaat gcttaatttg caaaaaaaac ttccaagcaa aaaccaatag aactttatat
     8161 tgctctgaag aatgccgtaa gaaaggcaat cgtgagaaac aacgtaaatt gatgaaacaa
     8221 aaacgggctg aacagaggaa agaaaaaaag aaagtcctaa atcctaacac agatgtgaca
     8281 gaaaagccta aaaaaatacg taatttagcg cagcactata aaaaactaaa aaaggaaatt
     8341 ttggcaaatg aatctgaatt tggttttact ggaataacac ttattgaagg aatagatgta
     8401 catgaagaaa actttgtaga tttagtcatg caaaaaataa aggagcagaa atgaattata
     8461 tgggtatggg ctatcttcgt aggaagttag ctctttttaa aactggagtt gataaaagat
     8521 atcgttatta tgccatggat gacagagacg acacacgaag tattgtcatg ccaaataatg
     8581 tgcgtgaaat gtacaggtct gtgttagaat ggaccgctaa aggggttgat agccttgcgg
     8641 accgtattat tttcagggag tttaccaacg atgattttaa tgcttgggaa atttttaaag
     8701 cgaataaccc tgatatcttt tttgatacag ccatacagtc agcattaatt gcatcttgtt
     8761 gctttgtgta catcatgcca ggagcggaag acggcttacc taaaatgcaa gttatcgaag
     8821 ctagtaaagc gacggggata cttgacccaa ctacattttt attaacagag ggttatgcaa
     8881 ttttagagtc tgactcaaac ggtaatccta cgttagaggc ctatttcaca gacaaagaca
     8941 tctggtatta tccaaaaaaa gggaaaccat ataatattaa aaatccaaca ggtcacccct
     9001 tgcttgtacc tatcattcac agaccagacg cagttagacc atttggtcgc agtcgcatta
     9061 ccaaggctgg aatgtatcat caaaaggcag cgaagagaac gcttgagaga gcagaggtta
     9121 cggctgagtt ttactcattc ccacaaaaat atgttttggg aatggatcca gacgctgaac
     9181 cgatggaaaa gtggcgtgct acggtgtcaa cgttactcga aatctcaaaa gacgaggatg
     9241 gcgacaagcc aacagttggg caattcacaa cagcaagcat ggcccctttc atggaacact
     9301 tgaaaatgta tgcttcgcta tttgctggtg gttccggtct cactcttgat gaccttggtt
     9361 ttccatctga caacccatcg tctgttgagt caataaaagc ggcacatgag aatttgagag
     9421 cagcaggacg caaagctcaa cgttcttttt cttctggatt tctaaatgtg gcgtatattg
     9481 ctgtttgttt aagagatgaa tttccttatc ttcgtaatca attcatggat acagtaatta
     9541 aatgggagcc tctctttgaa gctgatgcaa atatgctaac tttagtaggg gatggagcta
     9601 tcaaacttaa tcaagctatc cctggtttca tggatgcaga tgttatccgt gatttaacgg
     9661 gagtaaaagg ggctgataaa cctatacctg ctatcacgga ggtaacaact gatggttgat
     9721 gatgtcttac ctaagctact aaaatctgtt cgacaagatt ttgaaaagta ttttggcgaa
     9781 agtgatgttg taaccaaggc ttttgcagaa ttgcaagcta aaaaagtgac ctataaaaca
     9841 gtcaatgagt ttgctattga agtcggaaga cttttatctt tggctctgac aggttctgtt
     9901 agctcggata aattaccaga cggtaaaatg tattacaata tcgctaagcg tctcttagat
     9961 gagactatgg gacggaatta caaattgatt tcaggttatg ctggtgatgt tcagcggatt
    10021 ttaaatgaga atgctcagat tggtctaaag gtgcaacgtc caccgctaaa tcgagacaag
    10081 attaacggga tggtaaatcg tttggatagc gagaatacat ttgatgatgt aaaatggctg
    10141 tttggtgaac cgattgttaa tttttcgcag tccattgtgg atgataccat caaggcaaat
    10201 gcggatttac aatataaaac aggtatgacg ccacaggttg tcagaacaga aagtggcaac
    10261 tgttgtgaat ggtgtcgtga ggttgttggc acttatagtt atccaaaagt ccctaaggac
    10321 gtatggagaa gacatcagcg gtgtcgatgt acgcttgact atgacccgaa gaatggaaaa
    10381 gttcagagtg cttggagtaa aatctggcga aagaaagaaa aaactcaaga atctatagaa
    10441 agggttgaaa aatttaagga aagtgctcta gtagaatcta tcaaaaatga tatcgctaag
    10501 ttagatatga caaaggttgg tccgagtgat ataattgata tagggaaacg tatcaattat
    10561 cactttagag tttcagaaca tataggagat aaagagaaat taaaagaaat tttctcaaat
    10621 tttcgtgaaa tcggtggaga aatacctaaa aatacttggg caaaaggttc atctaaactt
    10681 gttaaagacc agctacaaga ggcttttcaa aattatccaa ctgagtgggc agctgttccg
    10741 gacggtattg gtaaaaaact aaaagctata aaaaggaagc gcggttactt cgatggatat
    10801 gatgaagatt tagttattgc tacaaatgga acaagaaaaa caacacctta ccatgagata
    10861 ggacatatga ttgaactggt taatcctgat ttagtaagat tagagaaagc ctgggtagat
    10921 aaaagaactg ctaatgaagc tgaggttcgc ttgaaagata tttttccaag ttcaaattat
    10981 ggtattgggg aagttactaa gaaagatgac tttatatcac catatattgg taaatactac
    11041 agcgatgcag ctgaagtttt cactatggga ttacaaggta tttttgttcc tgaagaacga
    11101 tttgctaagt cctttgacaa aaaaacatgg aaatatgact acaagacaat caatgacgac
    11161 cctgaatttt tgaattttat tattggatta tttgtgaaag tgtgataaat atggaaccaa
    11221 aattacatcg gcaactgcgt caaaaatatg acgacgctga aaaacaatat cttgaaaagt
    11281 ttggagaaga ctcgcttgat agagtatttt tttgggagcc agacgtttac tttgatgagt
    11341 ggaaaaaggt tctaccagat gcaacactgg aattaaacaa agctattaat agcggggtgg
    11401 cgattgatcc agatccagaa aacgcaatat attaagcacc tagagcaatc tggtgctttt
    11461 cttatgccca aaaaggagaa aagcatgaat aaaatatcat aaaatgtcga aatgacttgg
    11521 gaaaacaaag acgaatttca acaaatcatg aataaagtca atgaaacaaa agaagcttac
    11581 gaaaaagcct tagaagctgc ggaaaatttt gtccctaaaa tgtcgtgtgc aacaaagtta
    11641 aataaaggag aaggtaatga ataaacgtat caagaaaaaa cgtaaattgg aaacagctat
    11701 tatgctattg attgcagaaa atgccttgca ggctgaagca attaaaaatc aaaacaaaga
    11761 aatcatggag ttgaaatcaa ttgttcagcg gaacgctctg gcaacaaacg aagagttagc
    11821 gactgttaaa gctgctactt tagataacca atcagttatc aaggcaatcg gtgacacggt
    11881 tgactacatc aagaaaaact acaaacgtaa gtgggggaag tgatatgaaa tatcgtaaga
    11941 aaccggtcgt agttgaagca attcaattta cgggtacaaa ctatgaaaaa atcaaagagt
    12001 ttattggtca aaatacctta tgttcgactt tgagtattgt aatccccact cttgaaggcg
    12061 actacatcat caaaggcatc gctggtgaat tttatccatg taaacctgat gtttttgaac
    12121 agacttatga atcaacttaa accaaagtcg tagcaatacg gctttttatt gtgccctgtc
    12181 gcatggctaa aaactaggca gtacgattga aaggaataag tatggttact aagacgaaaa
    12241 caaagcttgg caatcagcga cctactcaat cggtaaattt acattttgct aaatctctag
    12301 cgcatgaagc tattaattac tacaaaaaaa cagggctaag ctgctatcca tggcaagtaa
    12361 atatgcttat cccaattatg gccatagatg aaaatggtct gtgggttcat caaaagtatg
    12421 ggtatgctat cccacggcgt aacggtaaga cggaagtagt ctatattgtt gagctgtggg
    12481 ctttgcataa aggtttaaaa atcttgcata cagctcatcg aattagcaca tctcatgcat
    12541 cattcgaaaa ggtaaaaaaa taccttgaga tgtcaggtta tgttgatgga gaagacttta
    12601 tatcaaataa agccaagggt caagagcgta tagagttcaa agccagcggc gctgttatcc
    12661 agttccgaac taggacatca aacggtggac ttggtgaggg atttgactta cttatcattg
    12721 acgaggcaca agaatacaca tctgagcaag aatcagcatt gaagtacaca gttactgata
    12781 gtgataatcc aatgactatt atgtgtggaa cgccgccaac gatggtatct actggtacag
    12841 tatttgaagc atatcggaaa gattgcttaa aaggcaataa gcgttattct ggttgggctg
    12901 aatggtcagt tcctgagatg gttaagatta acgatgtatc ttcctggtat atttccaacc
    12961 catctatggg attccacctc aacgagagga aaatcgaagc tgaattaggt gaagatgaga
    13021 ttgatcacaa catccaacgc ttaggttatt ggccatcctt taaccaaaaa tcagttatat
    13081 ccgaaaaaga atgggcaaaa ctcaaagttg agcaagtgcc agaactcaaa agcaagcttt
    13141 ttgtcggtat caagtttggt caagatggca acaacgtatc actatcaatt gcagcaagaa
    13201 catcagaaaa taaggtattt gtcgagacta ttgactgttt atcagttagg aatggtactc
    13261 aatggattat taattttctg aaatcagctg acattgctaa ggttgtaatt gatggcgcca
    13321 gcggccaaga attacttgct caggagatga aagagcaagg tctaaagaaa ccagaattgc
    13381 ctaaagttgc tgaaattatc acagctaaca tgatgtggga gcaggggatc atgcaggaaa
    13441 ccatttgtca tagtgatcag ccatctttga cagcagtagt cacaaactgt gaaaagaggc
    13501 aaattggctc taatggtggt tttgggtata agtcgcttta tgatgataga gacattagct
    13561 taatggacag tgcattgctt gcgcactgga tttgttacac aacgaagcca aaaagaaagc
    13621 aaagaaccag ctgttaaaaa acgacatccg aaagggtgtt tttttactgc taaaaaatct
    13681 accgaactgc cgggaaagca ggagaaagga cgttaatatg tcagaattta aagttattga
    13741 aacacaagaa gagttggaca cgattgtgaa agctcgcatt gctcgagaac gtgagaaata
    13801 tcaagattac gaccaactga aaactcgtgt tgaagaacta gaaaccgaaa acagcagctt
    13861 acaaactgct ttgaatgatg ctaaatcaaa cactgatagc tatacggaag agattagcac
    13921 cctgaagaat caaattgccg attatgagac ggcaaattta cggacaaagg tagcgttaca
    13981 gtatggctta ccaattgatt tagctgatcg tttgcaagga gatgatgaag atggactcaa
    14041 agtagatgca gaacgcttag catcctttat taagccatcc caaccacaac cgccagcaaa
    14101 atcaaacgaa ccaaatatcg atagtaatgc agacgcaaat tacagagcgt tagtgcaagg
    14161 attaagtaca gaagattaga agattaatta aaggagaaaa aatatgggaa cagaaacatc
    14221 aaaagcaagc ttatttgaca aacatttagt atcagatctt atcaataaag ttaaggggca
    14281 tagctcacta gctaaactat ctagccaaaa acctattccg tttaacggat ctaaagaatt
    14341 tacgtttaca ttagattctg atattgatgt ggttgctgaa aacggtaaaa aaacacacgg
    14401 tggcttatcg ctagagcctg ttactatcgt accaatcaag gttgagtatg gtgctcgtct
    14461 ttctgatgaa tttttatatg caacagaaga agaaaagatt gatattttga aagcttttaa
    14521 cgaagggttt gcgaaaaaac tcgctcgtgg tattgaccta atggcgatgc atggtatcaa
    14581 tccacgtaca aaaaaagcgt ctgacgttat cggcacgaat cactttgata gtaaagtgac
    14641 gcaagtggtt aaatttacag agagcgaaga cgctgatgca aatatcgaag cagctgttaa
    14701 tctaattcag ggggctgaag gtgtagtgac agggcttgcg atggatacag agttttcaac
    14761 agcactggcg aaagttacca atggggagat ggggcctaaa atgtaccctg aacttgcttg
    14821 gggagcgaat ccagatagca tcaatggctt gaaatcgtcc gtcaatacta ctgttggtgc
    14881 tggagctgat gaagcagaat ccaaagattt agtaattatt ggcgattttg aaagcatgtt
    14941 taagtggggt tatgcaaaac aaattccaat ggaaatcatt aaatacggtg atcctgataa
    15001 ttcggggaaa gaccttaaag ggtataacca aatttactta cgtgctgagg cgtatatcgg
    15061 ctggggtatt ttggacgcta agagctttgc tcgtgtcact aaaggagaag tataatgtta
    15121 tacgttaaca gtaagactgg agctagtttt tatagctccg tccctgtttg tggtggagat
    15181 tggactttgg ttgaacagca gacaggcgaa aaagcaaaaa cagtagcaga aattaaacgt
    15241 cagttagatg ctgctgggat aaaatatagc tctaaagcta agaaacctga attagaggca
    15301 ctcttgcctg gttaggaggt aaaagtgggc aattttgcaa caacagatga cgtcattttg
    15361 ttatggcgtc ccttatctgt tgacgaattg aaacgtgcaa atgcactctt gaaagtcgta
    15421 tcagatacat taagaatgga agctgacaaa gttggcaaag acttagataa aacgatggtt
    15481 gataagcctt attttgttaa tgttattaaa tcggttacgg ttgacattgt agctagaaca
    15541 ctcatgacat ctactcaagg tgaaccgatg tcgcaagaaa gtcaatcggc acttggctac
    15601 acttggtctg gaacttattt ggttccagga gggggactgt ttataaaaga caatgagctt
    15661 aaacggcttg gtctaaaaaa gcagaggtat ggaggtattg agctttatgg ggaaattaag
    15721 agggataacg attactttga tagataaagt aactatcgat atagaccctt tcggaaatcc
    15781 aataaaaaaa gataaagaaa tatctgtcga taatgtcctt gtatcgccag caacaagcga
    15841 tgacataaca agccagctta gcctttcagg aaaaaaagct gtttacactt tagctattcc
    15901 aaaaggagac aaccacgatt ggggagataa ggaagttaga ttctttggag aaaagtggcg
    15961 cactgtcggc ctagctcttg aaggtattga agagcttatt ccgcttgaat ggaataagaa
    16021 agttatggtg gaaagatatg agtaagttta aattcaagct caataaagct ggtgttgctg
    16081 aattgatgaa atcatcagaa atgcagcagg tattaaccac taaggccaca gccatcagag
    16141 aacgttgtgg tgatggttac gcccaagata tccatgtcgg gaaaaatagg gctaatgcta
    16201 tggtcagtgc taaaaccata aaggccaaga aagataattc aaaaaacaac acattgttga
    16261 aggcggtgcg atgattgatt gaagtaatta tcaaaaaata tttagacgag cacttagatg
    16321 tgccgtcttt ttttgaacat caaaaagatg aacctgcacg attcatcatc ttagaaaaga
    16381 ctagcggggc taagcaaaat catttgctaa gttccacgtt tgcttttcaa agttatgccg
    16441 aatcgttgta tgaggcggct ttacttaatg acaaagtaaa gcaagtaatt gagcagcttg
    16501 atgtcttgcc acaagtttct ggtgtacatc ttaacgctga ctacaatttt acagatacag
    16561 caactaagcg ttatcgatat caagctgtat ttgacattaa tcactattaa caaaaggaga
    16621 taataatggc aaatgatact aaaaatgtaa catctgcaaa gccaaaagca ggcggagcta
    16681 tttattctgc accgttgggg acaacattgc ccgaggatgc aaaatcaaaa ttagatacta
    16741 aatttaagaa tttgggttat gtatccgagg atggtgtggt caatgaagat acacgctcat
    16801 ctgaaaatat taaagcgtgg ggtggagata ttgttggatc tgtgcagaca gaaaaagagg
    16861 ataaatttac ttataagctg attgaatcac taaatgtaga agtcttaaaa gaagtttatg
    16921 gcgctgccaa tgttactgga gatttagata agggaatcca tattaaatca aattcaaaag
    16981 aacttgaagc tcatgctatc gttattgata tgatcatgaa tggtggaatt ctgaagcgca
    17041 tagttttacc gaatgcaaaa gtcgatgagg taggtgaaat taagtatgtt gacggcgaag
    17101 ttgtcggata cgaaacaaca ctaaaatgtt ttccagatga aaaaggagat actcatcatg
    17161 agtatattgt aaaacctgga gaagctaata aaaaagaaaa cagctttgaa atgtaaaggg
    17221 gagtaaatgg aaatcttaaa aggaaaaaca acatcaggat ttgaatacga aatccctaaa
    17281 aaacgattaa aaaactttga acttgttgaa gctattgcag aagaggaaac cgatccaaca
    17341 gcagtggtta aaatcgttaa tttgttactt ggtgatgctg ctaagtctct aaaagaacat
    17401 gtacgagatg cagaaggtat cgtagacgtt gaagctatcg gagtagaaat caaagaaatt
    17461 tttgaaagtc aaaaagattt aaaaaactag caatcctcgc tcagatgata gcaaaagatg
    17521 atgatgcatt gacttgtgat ttagctgaaa catacggcat atatgattac agacagctac
    17581 ctgcttatca ggtggctgtt tttgctgtcg gtttgagatc taactctagg ataaaaatgg
    17641 cattatctgg agagactgag gctttggata ctgttttgtt agctggtatt tacgataata
    17701 ctaatttgct gttttggtct aaaactaagg atggtcaatc tggtcaaaac aaacctaaat
    17761 ccgtggtgga agctatatct ggatctaaat cacaaaaagc taatgatgtc atttcttttg
    17821 cgtctggcga ggattttgaa aatgcacgta aacaattact aggaggtgat ggctaatggc
    17881 aacagaactt ggtcaagcgt atgtgcaaat tatgccatcc gctcgtggaa taagtggagc
    17941 aatctcaaag caacttgatc cagaagcaag gtcggctggt ttgagcgcag gtaaattgtt
    18001 tggtagtaaa ttgaaattag cggctctagc ggctactgct ggtttaggta ttttactcaa
    18061 gaaaacaatt tctgcatctt tgtccactgg tgctgactta cagcaatctt ttggtggtat
    18121 tgataccttg tataagggcg ctgagactgc tgtcaaaggg tttgctaaag aagcgtacaa
    18181 agctggtata tcagcaaata cttatgcaga gcaagcagtt tcaatgggtg catctctaaa
    18241 gcaatcactt ggaggtgatg ctgtcgcggc tgccaaggct gctaacatgg caatcatgga
    18301 tatggccgac aactcggcta agatgggtac tgatatcaca tcaatccaaa tggcttacca
    18361 gggatttgct aagcaaaact atacaatgct tgataaccta agacttgggt acggcggcac
    18421 aaaagaagag atgaagcgtc ttttatcaga cgctgaaaag ttacctgccg ctatgggcaa
    18481 gaagtttgat ttgagtaatt atgctgatgt ggttgaggct atacacttgg tacaggataa
    18541 catgggtatc gctggagttg ctgctgaaga agcaaaaact acattttcag gctcactagc
    18601 tgctatgaag tcctctttta caaatgtact ggcaggttta tcactaggag atgatataag
    18661 acctgcttta cgaggactgg ctgaaacaac ttctaatttc ttatttggta actttattcc
    18721 gatggtggca aatatcttta aaggattacc atcggcaatt ggtactttta ttggagcagc
    18781 aactcctatt attgccagtc aatttaaagg gctaatgagt agccttggga ttagtattga
    18841 tttaagtcct attactgcta aatttgcaca gattggccaa aatttacaac ctgtttttaa
    18901 cggtttaaaa acggctttta gtcagttgcc atcatttttt actagcattg gtagtgcagt
    18961 tgcaccagta atagacacta ttattagcgg attagccaga ttagatttca gtggttttga
    19021 ggctttaatt tcagcaatct taccagcaat tcaaacaggg ttccaaacgt tcatgtctat
    19081 tgtaggtcca gcgattagtc agcttgtaaa tagttttgtt aatctttgga atgcttgcca
    19141 acctctagta acaatattaa gtggcgcatt gatgccagct tttcaaattt taggagcttt
    19201 tctcggcgga gtcttacaag gaatccttgg cagtattagc tttgcatttg atgctttaaa
    19261 agttgttatt gagtttttaa cgccaattat tgattggctt gtacaaggtt ttaatgctat
    19321 ttcaccggca ttacaaatcg ttgcccagtg ggttggtaat gtcattggta tatttactag
    19381 cttaggtgca tctgggcaag gtttaagtag tatgctaagc agtgcttggg ggaacatcca
    19441 aacagtagtt tcgacagcta aaaatatgat aactttggcg attgatggta ttaaattggt
    19501 atttagcaat ctagggaatg ctggaaatat tttaaaagga ttactttcgg cagcgtggtc
    19561 agctatgcaa aatgcagtag tgatagctaa aggtattatc aacagtgcga taagcgctat
    19621 aaaaacagca tttagcagct ttggtaatct tgtatctagc gtaagtggaa ctataaaatc
    19681 tgttattggc agtttaaaaa atgcgtttta cagtttagca agtattgact tagttggtgc
    19741 aggacgtgca atcatgcaag gatttctaaa tggactaaaa tcgatgtggg gggcaatcac
    19801 cggctttgtt gggggtattg ctgactggat ccgtaagcac aaagggccta tctcatacga
    19861 tagggttttg ctaaaacccg caggtaaagt aatcatgcaa gggttcaaca atagcttgat
    19921 ggatggcttt aaagaggtta aatcaaatgt atctggcatg gctgacgatc ttgcaggcac
    19981 cataacaggt aaaagtctat ctctcggtat cgatgctaaa ccaagcgtca cagctgatga
    20041 cttactatca agcaatatta gtactaaaac tacagtcggt tctgctacaa gtgacttgtc
    20101 attatttttt gttaaggtgc ttgctctgtt gcaagatatc cttgataaaa atacggatgt
    20161 ctatctagac aaagaaaaag tcagcgctat tttatacgaa gaatttgcca aaatcatggc
    20221 tagagagggg attgcatgat accaaaggtt attattgatg gttttgatac ctctacaatc
    20281 cctaattgtg tcctaactgg ttatgatgta ggggatgttt tatcccctca ttttgtcgaa
    20341 aatgaagtgt atgggatgaa tggtactagt aggggattag agtcatacaa tgagtcgaaa
    20401 cccactctaa catggcattt gagcactttt gatgatgcag ttaatttagt gaatcattta
    20461 gacggacttg ggaaaatcat tgaattttgg catattccga agtcatttta ttattatgat
    20521 tgcctatctg tcaaaatcaa tgcggtaact atgaactcgt ggagagtcac acttaaactt
    20581 gcactatatc catttaggta tgcgaaaggt atctcagatg taacgattgc aggcaatgga
    20641 agtataaata atccaggaaa tgtttttagt gaacctaaga taattgttga gggtaccggc
    20701 aaaggaacac taaccattga caaacaggtc atggaattaa atttgtcagg taaagcaacg
    20761 attgagtgca aacatggcca acaatgcgtt tatgatggtg aaggtaatgt gaaaaactca
    20821 atccgaataa gaggaagatt ttttgaaata caacctggca cacaaggtat tgccgtaagc
    20881 ggaggcattg ccaggttaac aattagtcca aggtggaggt acaaggtttg atatcgatta
    20941 aagatgataa tacacctctt gtagcagcct ttgaagatga gattacacag gaagcgaata
    21001 gcgattataa attaagtttt aagtatccag ctaaacatga gtatcgcccc ttgatcaaaa
    21061 aaggaataat cctagaagct gatgacctac atggaactca gctttttagg gtttttgaga
    21121 ttactaagcg acatggctat atcaatgttt atggtaatca ggtcgccgat gatttaaatg
    21181 gctatgcaat tgactctatc agtgttgata gggtgcaagg tatgacagta atgtcagagt
    21241 tagcaggtaa tatcaagcgt gagcatcctt ttagcttttt tagtgatatt gacggtcgtc
    21301 acacatttaa tcaatcagac gtatctgtta tggacgcttt agctaatggc aagcactcaa
    21361 tcatggggca gtggggtggc gagcttgtac gtgataagta tcaggttaat ttgctcaaaa
    21421 aagctggtaa agacactgag accttattta tgtataagaa aaacctcaaa tcttatgagg
    21481 agacagatac tataaaaggt ctcatctcga tactgcatct cgttgcagaa gttgaggagc
    21541 agcgggaaga ggaatctaga gaagtttctg atgcagacat tggccacaat gaagttagca
    21601 aaaagaaaac gattagggta actgttgaga gcaagctcaa agacactcat ccgataattg
    21661 ttgaaaagac tatcaaggtg caggatcaag atgtcaaaac agaagaggac ttgcttgcat
    21721 atggtaagaa atactttgaa aaaactcttt gcgacatacc aggtaatagt ttaaaaattg
    21781 atgttactaa taactacgag ggcgctgtta ggctatttga cacagcaatt gtcttccacg
    21841 agctctatga cagagactta cgaatgcaaa tcactggcta tcggttcgcc cctatggcca
    21901 atcggttaaa atccatcatc tttggagaga ttaagaccaa cttagcaaaa caaattagca
    21961 atcaaattga caataaggta gctgaatcaa ctgctcaaca tgacgcagca tttgaagcaa
    22021 aattacaaaa gcagattgat aatgctaatc gtatttttga cacaaaagaa gctaaactcc
    22081 gtgaagagat tgaagatggc atcaaaaaag ctgaagctaa tgcagaggtc aaagttgctg
    22141 aggttaacgc taaagtgctg gaagctgagg agctagccaa ggcagtcgat gagcgactca
    22201 aaaaattttt atctgatgct gacactaaag agcaagattt tgataaaaaa cttgaagaat
    22261 ttagaacgtc tcttaaagac cttgaagttg atgaaaagca gattgatgat gctttggcca
    22321 aagccggttt tagcaaggat agcttagctg acattaaagc taaactggaa gacacgtctg
    22381 aaactgccac agttacagct aacattgttg gatcaactgg cggcacgttt tacaatcgca
    22441 acagactgga tggtgatact gacaaagtta tcacttttga acaaggttat attgacattg
    22501 cccataatgg tgaaggtttt gaagaaggca agacatacac tatcagcttt gaggcaacct
    22561 gcgagctact gcgtaaagta ggaatcacag tgacacaggc taacatgaaa ggtgctcgct
    22621 tagtgttaac acctaaaaat cccaaattag ttgtcgagag ttttggcttg actaaggata
    22681 ctgagactat caatgtctat ccgtttagct acacagtgct tgtaaccagc gactggtata
    22741 aatctaagca aatagattta aacgcgtcgg aggtgcagga attggccctt gagatggctt
    22801 ataaagatgt ggttgacggt aataatgcca cgatagcagg gcagtggtca gacagcccgc
    22861 aaatgatttt agatggaggt agttaatgac tgaaaatata ccattaagag tccaatttaa
    22921 gcgcatgagc gctgatgagt gggctcgtag tgatgtcatc ttgcttgagg gtgagatagg
    22981 ttttgagact gatacaggct atgctaaatt tggtaatggg aaaagcaagt ttagcgcact
    23041 caaatacctt accggaccaa aaggtcctaa aggagacact ggtttccaag gtaaaactgg
    23101 aggaactggt cctcggggcc ctgctggcaa gcctggaacg acagattata atcaactcca
    23161 aaataaacca aatctagatg cgtttgcacg aaaacaagaa actgatagta aaatcaccga
    23221 attaaaatca aataaagcag ataaaaacgc tgtttactta aaagcagagt caaatgcaaa
    23281 gctagacgaa aaattgagtt tgacaggcgg catagtgaca ggacaactac agtttaaacc
    23341 taatagtggt attaaaccct catcttccgt aggaggagcg attaacattg atatgtctaa
    23401 atcggaaggt gctgctatgg tgatgtatac aaataaagat actactgatg gaccattgat
    23461 gattttacgt tctgacaaag atacgtttga tcagtcagct caatttgtgg attacagcgg
    23521 taagactaat gctgtaaata ttgtaatgcg ccagccaagc gcacctaatt tttcctcggc
    23581 acttaatata accagtgcca acgaaggcgg tagtgcgatg caaattagag gcgtcgaaaa
    23641 agcgctagga acgctaaaaa ttactcacga aaacccaaac gttaaggcaa attacgatga
    23701 aaacgctgca gcgttatcca ttgatattgt caaaaagaca aacggtgaag gaacagccgc
    23761 tcaaggaatc tacatcaatt cctccacagg aacaactggt aaaatgctca gaatcagaaa
    23821 taaaaatgaa gacaaatttt atgtaggtcc agatggcggc tttcactcag gtgcaaattc
    23881 aactgtagct ggtaatctaa cagttaaaga tccaacatct ggaaaacatg ctgcgactaa
    23941 agattacgta gatgaaaaaa ttgctgagtt aaaaaaactc atactaaaaa aatagattaa
    24001 ggaggataaa tgagcagaga cccaacatat acaataaacg agcacgactt atcttttgca
    24061 gatggtcgtt tttatgtgac ctttaaggca gataagtcaa gtgagactgt gagacttaac
    24121 agtagttgcc ttggcaatac cataatcaaa aagctacagg tcgaggatga caatacaatg
    24181 cacgactttg taaagcctaa agttaccact caacaagctt ttggactagc tcagcaggtc
    24241 aaagagcttg atttacagct aaaagaccct aagtcagatt tgtggggcaa aatcaagttc
    24301 aataataagg caatgctagt cgagtacgcc aacaaagaga tgtcaagtgc cattgcgcaa
    24361 tcagctgagc agatattgtt acaagtcaaa tctattgatg atgaacgata ttccaaattt
    24421 gagcaaactc tgaatggtat caaacaaact gtcaaaagtg agtcagttga atccgcacgt
    24481 actcagctag catcaatgtt tgatagtcgt attagtggac ttgatggcaa atacagtcgt
    24541 ttaagccaaa caattgatag tcttagcagt cgtcttgatg atggtgttgg taactactca
    24601 acgctatctc aaaaggtaag tggcattgat ttacgagtta gtaatgcagc taatgatgtt
    24661 tctcgattgt ctcagacagc acaaggattg cagtcacaaa tcacaaatgc aaaccaaaat
    24721 tacagcagtt tgtctcagac tgtacaggga ctacaaacaa ctgtacgtga taatcaatca
    24781 aatgctacaa gtcggattaa tcagttaagt gacctgatca gcacaaaggt atcaaaaggt
    24841 gatgttgaga caactattgc tcagagttac gacaagatag ccttcgcaat cagggataaa
    24901 ctcccagcaa gcaaaatgtc tggcagtgag attatctcgg caatcaatct tgataggtct
    24961 ggggttaaaa tcactggaaa aaatatcact cttgatggta acagctacat cagcaacgct
    25021 gtcatcaaag atgctcatat tgctaacatg gatgccggta agattaatac tggttatctt
    25081 aatgctaata ggattgcaac cgaggccatt actggtgaga aaattaagat ggactatgcc
    25141 ttttttaata aactcactgc taacgaggga tattttagga cgttgtttgc caaagacatc
    25201 tttgcaacat cagtccaatc tgtaacacta tcagctagca aaattactgg aggtgtatta
    25261 gccgctacaa atggggcaag tcagtgggac ctaaataatg ccaatatgac ctttaatcga
    25321 gatgccacaa ttaattttaa tagcaaaaac aatgccttag tacgtaaaga tggcacacat
    25381 actgcctttg tacattttag taatgcgacg ccaaaaggct atagaggctc agcgttgtat
    25441 gcgtcaatcg ggataacctc atcaggagat ggcatcgaca gcgcttcgtc tggacgtttc
    25501 tgtggagtta ggtttttccg gtacgc
//
LOCUS       DAUCWO010000019        25209 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUCWO010000019 DAUCWO010000000
VERSION     DAUCWO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25209
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.019"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(546..932)
                     /gene="rplQ"
                     /locus_tag="VGY20_000747"
     CDS             complement(546..932)
                     /gene="rplQ"
                     /locus_tag="VGY20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ4273616.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYIRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(947..1885)
                     /locus_tag="VGY20_000748"
     CDS             complement(947..1885)
                     /locus_tag="VGY20_000748"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ4273617.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1931..2314)
                     /gene="rpsK"
                     /locus_tag="VGY20_000749"
     CDS             complement(1931..2314)
                     /gene="rpsK"
                     /locus_tag="VGY20_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ4273618.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2332..2697)
                     /gene="rpsM"
                     /locus_tag="VGY20_000750"
     CDS             complement(2332..2697)
                     /gene="rpsM"
                     /locus_tag="VGY20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ4273619.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2715..2831)
                     /gene="rpmJ"
                     /locus_tag="VGY20_000751"
     CDS             complement(2715..2831)
                     /gene="rpmJ"
                     /locus_tag="VGY20_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ4273620.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2857..3075)
                     /gene="infA"
                     /locus_tag="VGY20_000752"
     CDS             complement(2857..3075)
                     /gene="infA"
                     /locus_tag="VGY20_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ4273621.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3193..3831)
                     /locus_tag="VGY20_000753"
     CDS             complement(3193..3831)
                     /locus_tag="VGY20_000753"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ4273622.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3981..5285)
                     /gene="secY"
                     /locus_tag="VGY20_000754"
     CDS             complement(3981..5285)
                     /gene="secY"
                     /locus_tag="VGY20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ4273623.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5302..5742)
                     /gene="rplO"
                     /locus_tag="VGY20_000755"
     CDS             complement(5302..5742)
                     /gene="rplO"
                     /locus_tag="VGY20_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ4273624.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5957..6139)
                     /gene="rpmD"
                     /locus_tag="VGY20_000756"
     CDS             complement(5957..6139)
                     /gene="rpmD"
                     /locus_tag="VGY20_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ4273625.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6154..6648)
                     /gene="rpsE"
                     /locus_tag="VGY20_000757"
     CDS             complement(6154..6648)
                     /gene="rpsE"
                     /locus_tag="VGY20_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ4273626.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6667..7023)
                     /gene="rplR"
                     /locus_tag="VGY20_000758"
     CDS             complement(6667..7023)
                     /gene="rplR"
                     /locus_tag="VGY20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ4273627.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7128..7664)
                     /gene="rplF"
                     /locus_tag="VGY20_000759"
     CDS             complement(7128..7664)
                     /gene="rplF"
                     /locus_tag="VGY20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ4273628.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7867..8265)
                     /gene="rpsH"
                     /locus_tag="VGY20_000760"
     CDS             complement(7867..8265)
                     /gene="rpsH"
                     /locus_tag="VGY20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ4273629.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8416..8601)
                     /locus_tag="VGY20_000761"
     CDS             complement(8416..8601)
                     /locus_tag="VGY20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ4273630.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8617..9159)
                     /gene="rplE"
                     /locus_tag="VGY20_000762"
     CDS             complement(8617..9159)
                     /gene="rplE"
                     /locus_tag="VGY20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ4273631.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9183..9488)
                     /gene="rplX"
                     /locus_tag="VGY20_000763"
     CDS             complement(9183..9488)
                     /gene="rplX"
                     /locus_tag="VGY20_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ4273632.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9567..9935)
                     /gene="rplN"
                     /locus_tag="VGY20_000764"
     CDS             complement(9567..9935)
                     /gene="rplN"
                     /locus_tag="VGY20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ4273633.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9960..10220)
                     /gene="rpsQ"
                     /locus_tag="VGY20_000765"
     CDS             complement(9960..10220)
                     /gene="rpsQ"
                     /locus_tag="VGY20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ4273634.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10246..10452)
                     /locus_tag="VGY20_000766"
     CDS             complement(10246..10452)
                     /locus_tag="VGY20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ4273635.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10462..10875)
                     /gene="rplP"
                     /locus_tag="VGY20_000767"
     CDS             complement(10462..10875)
                     /gene="rplP"
                     /locus_tag="VGY20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ4273636.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10879..11532)
                     /gene="rpsC"
                     /locus_tag="VGY20_000768"
     CDS             complement(10879..11532)
                     /gene="rpsC"
                     /locus_tag="VGY20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ4273637.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11545..11889)
                     /gene="rplV"
                     /locus_tag="VGY20_000769"
     CDS             complement(11545..11889)
                     /gene="rplV"
                     /locus_tag="VGY20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ4273638.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11905..12183)
                     /gene="rpsS"
                     /locus_tag="VGY20_000770"
     CDS             complement(11905..12183)
                     /gene="rpsS"
                     /locus_tag="VGY20_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ4273639.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12322..13155)
                     /gene="rplB"
                     /locus_tag="VGY20_000771"
     CDS             complement(12322..13155)
                     /gene="rplB"
                     /locus_tag="VGY20_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ4273640.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13173..13469)
                     /locus_tag="VGY20_000772"
     CDS             complement(13173..13469)
                     /locus_tag="VGY20_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ4273641.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13469..14092)
                     /gene="rplD"
                     /locus_tag="VGY20_000773"
     CDS             complement(13469..14092)
                     /gene="rplD"
                     /locus_tag="VGY20_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ4273642.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14116..14742)
                     /gene="rplC"
                     /locus_tag="VGY20_000774"
     CDS             complement(14116..14742)
                     /gene="rplC"
                     /locus_tag="VGY20_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ4273643.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14990..15298)
                     /gene="rpsJ"
                     /locus_tag="VGY20_000775"
     CDS             complement(14990..15298)
                     /gene="rpsJ"
                     /locus_tag="VGY20_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ4273644.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15497..16786)
                     /locus_tag="VGY20_000776"
     CDS             complement(15497..16786)
                     /locus_tag="VGY20_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ4273645.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGMFLLCQFIPIKQVVKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKFPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITSH"
     gene            complement(17174..18190)
                     /gene="adhP"
                     /locus_tag="VGY20_000777"
     CDS             complement(17174..18190)
                     /gene="adhP"
                     /locus_tag="VGY20_000777"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ4273646.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18442..21084)
                     /gene="adhE"
                     /locus_tag="VGY20_000778"
                     /gene_synonym="adhC"
     CDS             complement(18442..21084)
                     /gene="adhE"
                     /locus_tag="VGY20_000778"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ4273647.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
     gene            complement(21393..23168)
                     /locus_tag="VGY20_000779"
     CDS             complement(21393..23168)
                     /locus_tag="VGY20_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ4273648.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLFWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMVGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGTLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPNGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
     gene            complement(23165..23566)
                     /locus_tag="VGY20_000780"
     CDS             complement(23165..23566)
                     /locus_tag="VGY20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273649.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRIHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            complement(23589..24026)
                     /locus_tag="VGY20_000781"
     CDS             complement(23589..24026)
                     /locus_tag="VGY20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ4273650.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            complement(24164..25162)
                     /gene="ruvB"
                     /locus_tag="VGY20_000782"
     CDS             complement(24164..25162)
                     /gene="ruvB"
                     /locus_tag="VGY20_000782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ4273651.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
BASE COUNT         7440 a         5317 c         4439 g         8013 t
ORIGIN      
        1 actttttttg tactttttta caattttgtt ggaaacggct taaagtgaca gattttaaaa
       61 gtaaaaaaga tattttcacc tccaaattaa aagaggattt taaaatactt attaataaaa
      121 tattgaatag gagataaaac taacaatata gtttgagcat ctgtttgcga tgcaagtttt
      181 tttcggacat ttaaatgagg gggttacacc gtttatttgc ccatgagagg tctttaaaca
      241 cctgcataag gttttcttat tctaatgacc ttattgtctc tgagaaaact ttataaacgc
      301 atcagaagag gcttctgtgt tattgttttg ctcagcttaa aagcgggggg gtcacctcat
      361 aatacaacaa aacgcctgct tataaaaagt ttctatagct tagatacctt tcataaacaa
      421 gcgttacata ttaacaatat gatgagtgtt cccgctagga aaaatcctag cggtagacca
      481 gagctagact aagaataatc tctcgattac tccatcatca taacactcaa caaaattgat
      541 aaaaattaaa ctaattcaat aattgccatt ggagcagcat ctccacggcg tggttctgtt
      601 ttaagaatac gagtgtatcc accgttacgt tctgcataac gaggtgcaat ttctgagaaa
      661 agtttttgaa gagctgtcgt tgatgtgtat ttatcggtag cttcatcata attttctgat
      721 gcgatttcgt tacgaatgta tgcagctgct tggcgacgag catgaagatc accacgttta
      781 ccaagagtaa tcattttttc aactgtttta cggatttctt ttgcacgtgc ttctgtcgtt
      841 acaattgatt cgttgatcaa aagatctgtc gtcaaatcac gaagcattgc tttacgttgt
      901 gagctagtgc gtcctagttt acggtaagcc attttgtcct cctatattat ttatcgtttt
      961 ttagtccgag acctaagtca gcaagtttaa ccttaacttc ttcaaggctc ttacgtccaa
     1021 gattacggac tttcatcatt tcaggctcag atttttctgt taagtcgaag acagtgttaa
     1081 tgcctgcacg ttttaaacag ttatatgagc gtacggataa atcaagctcc tcgatggtgc
     1141 ggtcaagtac cttttcatcg ttcacttttt cagtttcttt cattacttcg gtagcttttg
     1201 caacctctgt taaatcagta aacaggttta agtgttcgat caaaactcga gcagatagac
     1261 ctaaagcatc ttcaggaata attgttccgt ttgtcatgat ttcaatcgtt aatttatcaa
     1321 agccatcatt gctacctaca cgggcaggtt caacttgata attaactttt ttaactggcg
     1381 tatagattga atctactgcc aatgtaccca caggtgcatc atcttttttg ttgccttctg
     1441 ctggcacata accacgtttt ttagcaacag tcattgttgc acgtagagaa tgtccttcag
     1501 cgattgtaaa aagataatga tccgggttaa caagttcgat atcactgtca gtaagaatat
     1561 caccagctgt tacctctgct ggtccttcta cttcaagttc gataatcttt tcgtcttcga
     1621 cgtatgattt cacagcaagt cccttaacat taaggataat ttgcatgaca tcttcacgta
     1681 cacctgggat tgtatcaaat tcgtgtagta ctccatcaat tttaattgat gttactgctg
     1741 cacctggaag tgaagacaag agtacacgac gaagagaatt acctagagtt gttccgtagc
     1801 cacgttcaag tggttcgatg acaaatctac cgtaatcttt attttcatca atttttgtta
     1861 ttattggttt ttcaaactca atcatttttt cacccctcga aacgaaactt gtgtactatt
     1921 tgttatatga ttatacacga cgacgttttg gaggacgagc accattatgt ggcacagggg
     1981 tcacatcacg aattgcagtt acttcaagac ctgcagctgc tagagcacga atagctgatt
     2041 cacgacctga accaggacct tttacagtca cttcaacagt ttttagtccg tgttcttgtg
     2101 cagattttgc agctgcttct gcagccattt gagcagcaaa tggagtagat ttacgagaac
     2161 ctttgaaacc aagagcacca gctgatgacc atgcaagagc attaccatgc acatctgtaa
     2221 tcataacaat agtgttatta aatgtagcgt gaatgtgtgc aacaccagat tcgatgttct
     2281 ttttcacacg acgtttacgt gttggtttag ccaattgttt tacctcctat tttatttttt
     2341 cttacctgca atcgcaacag ctttcccttt acgagtgcga gcattgtttt tagtattttg
     2401 tccacggaca ggaagtccac gacgatgacg gattccacgg tatgaaccga tttccatcaa
     2461 acgtttaatg ttcatgttta cttcacgtcg aaggtcacct tcaactttga ttgcatccac
     2521 ttcacgacgg atagcatctt cttgatctga tgttaaatct ttaacacgga tatcttcaga
     2581 aatacctgca gctgctaaga tttttttaga tgttgcaaga ccaattccgt aaacataagt
     2641 aagtgaaatt actacgcgtt tatcatttgg aatatcaact ccagcaatac gagccatttt
     2701 ttctcctttc tattttatcc ttgacgttgt ttgtgttttg gatttgtcgg acaaattacc
     2761 ataacacgac cattacggcg aataacttta cagtattcgc aaattggttt aaccgatggt
     2821 cttaccttca tgttttaatc cctccaatta tttcgattat ttaaagcggt atgtgatgcg
     2881 tccacgtgtc aagtcataag gactcatttc tactgtaaca cggtcaccta ctaaaatacg
     2941 aatgtaattt ttgcggattt ttcctgatac agttgctaga atttgatgtc cattttccaa
     3001 ttcaacagta aacattgcat ttggcatcgt ttctactact ttgccttcaa tttcaatcac
     3061 gtcttctttt gccacgcaaa agtaccccct aaatttcgat ttgatgtcct ctgagcacag
     3121 aggtaacaat tataagtcag actaaactag tatatcatta ctaatctact tatgcaagta
     3181 gattagtata atttatttga gttctagcaa cgctttttca acatctgcaa aaacgtctgt
     3241 tatttcttga ttaccttcaa tatctgtaac aagaccaagc ttacgatagt gttcaagaat
     3301 aggttctcct tgagccatat tgacgtccaa acgacgttta acagtttcag gcttatcatc
     3361 ttcacgttga tagtaatctt cttctttata atctactggt gggttgaaca ctttatggaa
     3421 cgtttcacca gttttacgat tgataatacg accactcaaa cgctctacaa gacatgatgg
     3481 atccacttta atattaataa caccatctaa gcggagtcct agttcttcaa gtgttgcatc
     3541 taaggcgtgt gcttgttcaa tagtacgtgg atacccatca agcaaaaaac ctttttctgc
     3601 gatatcatcc tcagctaagc gctcttttac aatcccgttt gtgacttcat caggaaccaa
     3661 ttcaccttta tcaatataac ttttagctaa acgtcccatt tcggtttgat tagccattgc
     3721 ggcgcggaac atatcccccg ttgagatgtg agcaacacca aattcttcaa caatcttagc
     3781 tgcttgggtc cctttaccag ctcccggcaa acccatgatt aaaagattca tcttttcctc
     3841 ctcttttaaa ataaatacat agtaggaaat tattttcctt ggtttttgtt ttcaaatggc
     3901 aacaagaaat cgtttgatga taaccaagtc ttattgtcat ttaaaaacaa aatagaaggt
     3961 tgacaatgtc aacctgtatt ctattctgct gtattcataa atccgacata ttttctcttt
     4021 agaagatatc cctcaagctg tttcatacct tcgataccag ttgaaatcaa aatgagcaaa
     4081 ctagttccac ctaaagcaat actagaagaa aggttgagag cttgttgtgc tgctattggc
     4141 gctaaagaga taaatgctaa gaagatagct cctacggttg ctagtttttt tagcaatgcg
     4201 gacataaatt gttctgtctc acgtccaggg cgaacacttg gtatatatga ggaattcttc
     4261 tgaagatttt ctgctgtctt ctcaggatta acttgtacaa aggtatagaa gaatgagaac
     4321 aatataatca acaaggcgta aactatcatt ccgactggag tttgataatt aaaaatttct
     4381 tgcaaacggt ttaaccacgg taaatctcta ccattttgaa caaaaggaat aatcgtactt
     4441 gggatagttg taatcgagct agcaaagata acgggaataa cgccggctgg atttactttt
     4501 aatggaagat atgaacttgt aggtgcacct tgcattagct ttgtgtattg gattggaatt
     4561 ttatattccg cttgttggac atatgttgta aagaaaacaa tagcaagaac agctatgatt
     4621 aaaatcccaa caatgagata agacgagtgt aaatcacttg ccttaacatt tacaaaatag
     4681 tcttcacgaa ttgttgcaat tgcacttgga atagacgaaa taatacccgc aaagataatc
     4741 attgatacgc cattaccaaa tcctttatct gtgatttgtt ctccaagcca ggtaacaata
     4801 acgctacctg ttgtcagtaa tgccccgatc agtaaatatg ttttgatgtc tggtgtctta
     4861 acaagtgcaa cgttcgacaa agtattaaac cctgcagtga taccaatgga ttgggcaaaa
     4921 gccaaaacaa gtgaaatata gcgcgtcgct tgatttaatt tacgacgtcc aacctcacct
     4981 tgtttgcccc actcaacaaa cttaggtaaa atatccatct gcaataactg aacaacgata
     5041 gaagcagtaa tatatgggct aacccccatt gaaaatactg agaaatttct catcgcatta
     5101 ccactaacta agtttaacat atttaagaaa gggagttcac ttaattgctc taagctcttg
     5161 gcatttacac caggtacggt aatatgtgtc ccaattcgga atacaagaat gataaatata
     5221 gtaaagaaaa ttttattccg tacagtcttt atctttaacg cgtcttttag tatttttaag
     5281 aacataatga gtatcccctc attagatgac ttcgattgaa ccacctttag caataatagc
     5341 tgcttcagca gattttgaga acttagctgc ttttacagtc aattttttag tcaattcacc
     5401 gttaccaagc actttaacgc ctgatttttc agctcggaca ataccagcat cttttaaaat
     5461 agccggagtc acttctgtac catcatcaaa aacattcagt tgatcaagat tcacaagtgc
     5521 atattcttta gtattaatat ttgtaaagcc acgttttgga atacgacgga acaatggtgt
     5581 ttgtccacct tcaaaaccta aacgtacacc gccaccgcta cgagcttttt gacctttttg
     5641 accgcgacca gatgttttac cattacctga tgatgtccca cgaccaacac ggttacgtac
     5701 tttacgtgag ccttcagcag cttttaattc atgaagtttc attttttctc ctttttttgt
     5761 aaaatgctag cgcctctatg agggaaaagg actccctata gaaactcgcc tatacattaa
     5821 tgatacttaa gttatccaaa ataacttaat acagatacgc actaaccgac tactgtttgt
     5881 tgccaaaaaa gcaaactaca agagtcggtt ttgtgctcgt ttttaattat gaaatcagag
     5941 atttcacagt tataacttat ttaacatctt caacggtaac caagtgagaa atagctgtca
     6001 ccataccacg gatagcagca ttatcttctt tgactactga agagtttaat ttaccaagtc
     6061 caagagcaac aacagtttta cgttgttctg gcttacgtcc gataggagac ttagtcaaag
     6121 taattttaat ttgagccatg atgatcccct ttcttatgct aagtcagaaa ctgagatgcc
     6181 acgaagtgcg gcaacttctt ctgcacgttt aagttgtttc aatccttcaa cagttgcacg
     6241 aacaatgttg attggagtgt ttgaaccaag agattttgaa gtaatatcag caacacctgc
     6301 taattcgatg acggcacgaa ctgcaccacc agcagcaact ccagaacctt ctacagctgg
     6361 tttcaataat actttagctc caccgaagtt agtgtaaact tcgtgaggga ttgttgtacc
     6421 aaccattggt acttcgatca tgtttttctt agcagcttca actgctttac ggatagcttc
     6481 tggaacttct tgagctttac cagtaccaaa tccaacacga ccatttccat caccaacaac
     6541 tacaagagct gcaaagcgaa gacgacgtcc acctttaaca acttttgtaa cgcggttaat
     6601 cgcaacaacg cgttcttcaa gttcaactgc attatcttta aatgccatta ttttgtgtcc
     6661 tccctattag aatttcaatc cgttttcacg agctgcatca gccaaggctt taacacgtcc
     6721 gtgatagaga tatccaccgc ggtcaaacac cacttcagaa atacctttag ccactgcacg
     6781 ttcagcaaca agtttgccga ctacaacggc ttgttctgtt tttgttcctt tagaaacgtc
     6841 tttatcaaga gttgatgcac ttgcgagcgt tacacccgct acgtcatcaa taacttgagc
     6901 gtagatgcct gtattagaac ggaatacgtt caaacgtggg cgatcagcag ttccagagag
     6961 tttaccgcgg acgcgacggt ggcgtttttg gcggattttg tttttatctg gtttcgaaat
     7021 cacaattttc acctcttaat tttaatcaat ttgtctcatt gacaagttgt cgattgaact
     7081 tatcgagcat gttaaggtgc aatacttccc atacccgact aagaacatta tttacctgtt
     7141 ttaccttctt taaggcgtac gtattcacca acgtaacgga tccctttgcc tttgtaaggc
     7201 tctggtgaac gcaagctacg gatgtaagca gctgtttgac caacaacttc tttgttgatt
     7261 ccttcaactg agattgaagt tgggttagca acagtgaaag taattccttc tggagcttca
     7321 acttcgtctt ggtgagattt acctactgaa aggacaagtt tagtaccttg aagttgagca
     7381 cggtaaccga cacccttcat ttcaagatct tttttgaaac cttcagaaac acctacaacc
     7441 atgttattca agttagcacg ggttgtccca tggattgttt tcatttcttt tgagtcgtta
     7501 ggacgtacaa ctgtgatttc agtcccttca actttgattt caatattttt gttgaactca
     7561 cgagtgagtt cgcctttagg gcctttaaca gtaataacat tgttgttatt tgttaattca
     7621 acgcctgcag gcatagtaat tactttatta ccaatacgtg acatgtttat tttctcctgt
     7681 taaattgtca agccgcatag cgactagttt tcacgggggt ttgatagaat gatatacttc
     7741 aatttatatt tcaaaattga acacgagctg tggtctgtgt gaaaaagata aatcttcctt
     7801 gagtgtaaac acgctacgtc agatttccta ttttcacttt gtccacgacg ctcttagtat
     7861 cttaaattac caaacgtaag cgatcacttc tccaccaaca ttcttttggc gagcttcttt
     7921 gtcagtcaaa agaccttctg atgttgaaat gattgcaatt ccaagtccgt taagaacttt
     7981 aggcatgtca tcacgttttg cgtaaacgcg aagacctggt tttgaaatac gttttaagtt
     8041 agtgataaca cgctcaccat tttttccgta ttttaagaac acacgaatga tgccttgttt
     8101 atcatcttcg ataacttcaa cattttttac aaaaccttca cgtttaagaa tctcagcaat
     8161 ccctttttta atgtttgaag caggtacttc taacacttcg tgttttactt gattagcatt
     8221 acggatacgt gttaaaaagt ctgcaattgg gtcagtcata accatttttt atattctcct
     8281 cttactagca gtttgatgct atcacttact agttaatgtt gtccttaaag gactttggta
     8341 tcagcttatg ctcacaccgt tttaattaag cacaagactt aatctgaaga acaaattaag
     8401 ttttgtgtca tatttttacc aagaagcttt gacaacacct ggaatttgac ctttgtaagc
     8461 caattcacgg aagcaaacac gacaaagttt aaacttgcgg taaactgaat gtggacgtcc
     8521 acatttttca cagcgagtat aagcttgtgt agagtgtttt gcaggacgtt tgttcttagc
     8581 aatcatagat tttttagcca atttatttac cccctattat tttgcaaaag gcattccaag
     8641 gcccttaagc aattcacgtg attcttcgtc agtatttgca gtagtgacga ttacaatatc
     8701 aagaccacgt actttatcaa catcatcaaa gctgatttct gggaagataa gttgttcttt
     8761 cacaccgaga gtgtagtttc cgcgtccatc aaacgatttt gttggaacgc catggaagtc
     8821 acgtacacga ggaagtgaaa cgctaaccaa tttgtctagg aattcgtaca tacgttcgcc
     8881 acggagggta acttttgcac cgatcgctac accttcacga agacggaagc cagcgattga
     8941 tttcttagct ttagtaataa gtggtttttg acctgagata agtgctaatt cagctgcagc
     9001 tttctctagg ttttttgcgt tggatacagc atcaccaaca cccatattga gaacaatttt
     9061 ctcaactttt ggcacagcca taactgatgt gtaattgaat ttctctgtca acgcaggaat
     9121 tacttcgtta gtatattttt cttttaaacg atttgccatt atgcttctcc tttccttcgt
     9181 gattaatcaa gcacttcgcc tgattttttg ctgtaacgta cttttttacc gtcaacaact
     9241 ttataaccaa cacgtccagc tacaccattt ttatctaata cttgaacatt agagacatgg
     9301 ataggtgctt ctttttcgac gatagctcct tgaggatttt cagtgttagg tttttggtgt
     9361 tttttaatca ttccaacacc ttcaacaata actttgttaa cttttggaag tgctttaaga
     9421 actacagctt cagtgccttt gtccttgcca gcaattacgc gaactttgtc gccttttttt
     9481 acaaacattc tggtttctcc tattatttct tacgccccga tgggcaccct aggaaaacct
     9541 aggggactac gtttgttaac ctgagattaa agtacttctg gtgcaagtga tacgatcttc
     9601 atgtagccac cctcacgcaa ttcgcgtgca actgggccaa agatacgagt tccgcgagga
     9661 gttttatcat cacggatgat tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct
     9721 ggacggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc acctttttta
     9781 actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc gatgttagcg
     9841 aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc accgctatta
     9901 tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt ttagtttgat
     9961 tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt agctgaaagt
    10021 gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt ttcgtcatgt
    10081 gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt acgtttagtt
    10141 tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt
    10201 ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt tcatttctga
    10261 ttgcacagtt ttaacacgtg caatttgttt cttaacttcg tcaaggcgag cagtcttttc
    10321 aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga gttcgttttc
    10381 tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat ctttaatttc
    10441 ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc acttaactgg
    10501 taatttgtgg ctagcaagac gtaatgcttc gcgagcaatt tcttcagaaa caccagcaat
    10561 ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg cccctttacc
    10621 agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga agattttaat
    10681 ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag cttcgatttg
    10741 acggtttgta atccagtggc tagttgtggc ttgaagtccg tattcaccga atgaaacttc
    10801 tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt gtttaacacg
    10861 tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg gaagaacttc
    10921 tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag cttcttccca
    10981 agcatagtcg atatcagcgc gaagcgtgtg aagtggaaca gttccttctg aataaccttc
    11041 agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa tccctttagc
    11101 tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag caacacgttg
    11161 ctcaagttga cgagcaatat tttcaccaac aaggtgagca tctaaatcag gttgtttgat
    11221 ttcgatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac cacgaagagc
    11281 gtcaacgttt gcgccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac
    11341 aataactttg tttactgcac gttcaatttc aatagttgaa actgatgcgt cagctaattc
    11401 tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc
    11461 agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa ttggatgtac
    11521 tttttgaccc acgattttac ctccttattt ttctgataca actacagtta cgtgagttgt
    11581 acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg
    11641 tccttcgtta gcgaatgttt cagatactac caagtttgct ttttccaaac caaagttgtt
    11701 ttctgcgtta gcaattgctg agttaagagt tttctcaata acacgagctg ctttgtttgg
    11761 agtgaatttt aagattgcga ttgcgtcagc aactttctta ccacggataa gatcaagtac
    11821 taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag ctgaagtaat
    11881 ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc
    11941 tttgtaagta cgcgttggtg caaattcacc aagtttgtga cctaccatgt cttcttggat
    12001 gtaaacaggt acatgtttac gtccgtcata aactgcgatt gtatatccga tgaaacttgg
    12061 gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt cgtttgcttg
    12121 agcttcaact tttttcatca aatgctcatc gacgaaaggt ccttttttaa gactacgtcc
    12181 cattttgtag ttttctcctt taaataatgt accacagcgg cttgaattca agagcaagac
    12241 tccaaactct accgagttgg cggaataaat agtaaagcta ttgtaacgct atttcagcaa
    12301 gttttaagca acttactaaa attatttttc gttacgacga cgaacgataa gtttgtctga
    12361 tttagctttc ttgttacgag ttttaagacc aagcgctggt ttaccccatg gagttgatgg
    12421 cgctttacgt ccaactggtg ctttaccttc accaccaccg tgtgggtgat cgttagggtt
    12481 cattacagaa ccacgaactg ttgggcggat acctttccaa cgactacgtc ctgctttacc
    12541 aatgttaaca agtgattgtt gttcgttacc aacagtaccg atagttgcac ggcatgtacc
    12601 aagaatcata cgaacttcgc ctgattgaag acgaacaaga acgtatttac cttcttgacc
    12661 aagtacttga gcagaagctc cagctgcacg aacaagttct ccacctttac caggtttcaa
    12721 ctcaatattg tgaacaactg taccgacagg aatgtttgct aatggaagtg cgttaccaac
    12781 tttgatgtcg gcatctggac cagaaacaat acgttgacct acttcaagac ctttaggtgc
    12841 aatgatgtaa gctttcacac cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt
    12901 tggatcatat tcgattgttt taacaactgc ttcaacgcca tctttgttac gtttgaagtc
    12961 gatcaaacgg taatgacgtt tgtgtccacc accttggtga cgaactgtga tgcgaccatt
    13021 gttgttacga ccagctttgc tcttaagaga aacaagcaag gatttctcag gcgtgcttgt
    13081 tgtgatttcc gcgaaatcca aagaagtcat gttacgacgg ccatttgtcg ttggtttata
    13141 aactttaata cccacgttat ttcctcctta gattattcag cttcagctgc gaacaactcg
    13201 attgctttag aatcagctgt aagagtgatg atagcttttt tagtttttga agtgaaacct
    13261 gtgtaacgac caacgcgttt tgcttttggt ttaacgttaa cagtatttac acttgcaact
    13321 ttaactccgt caaaagcagc ttcaacagct tgtttgatca aaagtttgtg tgcacgagta
    13381 tcaacttcga aagtgtattt acctgcttca agagcaatca ttgacttctc agtgataact
    13441 ggttttttga ttacgtcgta caaattcatt atgcaagaac ctcctcaatt gtagagattg
    13501 cttctttagt aacaagaagt ttgtctgcgt tcacgatatc aagaacactt gcagttgctg
    13561 cagttgcaac agttacgttt ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt
    13621 cttcaacaag aacaagtact tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt
    13681 cagcagtttt tggtgctgca aatgaaaggc cttctacagc tacaaattta tcttcagcaa
    13741 cttttgctga gtaaactgat ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc
    13801 cgtatgaacg gggagttggt ccaaagacaa caccaccacc acgccattgt ggtgagcgga
    13861 tagaaccttg acgagcgcgt ccagttcctt tttgacgcca tggtttacgt ccaccacctg
    13921 atactgctga acggttttta accgcatgag taccttggcg aaggctagcg cgttggctaa
    13981 ttacaacatc aaaaacaact gattcgtttg gttcgatacc gaagatagcg tcgtttaatt
    14041 caactgagct aacttcttta ccagtttggt caaatagttt tacgtttgcc attttaactg
    14101 ttttctcctt tcttattatt tagcagcttt aactgctgac ttgatagtga taagagattt
    14161 cttagcacct ggtacgttac ctttaataag gataacgttc ttttctggga taacttgtac
    14221 aatttcaagg ttttgaactg ttacacggtt accacccata cgtcctgcca agcgtttgtt
    14281 tttgaaaacg cggttaggcg caacaggtcc catagaacct gggcgacgat ggtaacgaga
    14341 accgtgagcc ataggaccac gtgattgacc atggcgtttg ataacacctt ggaaaccttt
    14401 accttttgat gtccctgtga cgtcaacaac atcaccagct tcaaattgtt ctacagataa
    14461 ttctgcacca acttctaagc cttcaatgtt tttgaattca cgaatgaagc gcttaggagc
    14521 tgtgtttgct tttgcaacat ggcctttggc aggtttgtta ctcaagactt cacgtttgtc
    14581 atcaaaacca acctgaactg cttcataacc gtctgtttca acagttttaa cttgaagcac
    14641 aacgtttgga gttgcttcaa tgacagtaac agggatgaat tcgcctgatt cagtgaagat
    14701 ttgagtcatt cccacttttt tccctaagat tccttttgtc atgagaaaat atttcctttt
    14761 cttaataata tttcaaaaag ttttttatga gcgttttttg tgctcaaaaa agttttttac
    14821 gagcgttttt tgtgctcata gcaagatacg aagagcgtca aactcaaaat aaaaatagaa
    14881 aatctaacac aaaaacttct gtttcgagga agatttatct ttttcatgta gagtttagct
    14941 cgtgttcaag ttccatcaaa cactaactgt acttgcaaaa tctcaccgat taaagtttga
    15001 tttctacgtt gacaccactt ggaagatcaa gtttcatcaa agcatcaaca gttttctgtg
    15061 ttggattgat gatgtctacc aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat
    15121 ctttgtattt gtgagtcgca cgaataattg tgtaaagact gcgttcagtt ggaagtggaa
    15181 ctggtccagc aactgtagca cctgtacgtg ttgcagtttc aacgattttt tctgccgctg
    15241 tatcaagtgt acggtgttcg tacgctttca aacggatacg gatttttttg tttgccatct
    15301 tttctcctct cgtctattta cgataatagg ctagctcctc aagaaaaccg acacctgttg
    15361 cgtggcaatg caaccgagcg tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta
    15421 cagcaccaca atataataac agaatcattt agtcattgca agctttttag gcattttttt
    15481 caaaaaaact accttcctaa tgagaggtaa tttcttggta ctttttaaat tgtttagaga
    15541 gaataaacca ccgactggta ttatctaaca ctgtcgccat ccagacccct atgagtccat
    15601 attgccaaac cacaccaatc acataaccaa ggccaatgcg aatcacccac attcctatgg
    15661 ttgttgcata gaaaggaaac tttgcttttc ccaaaccctg ccagacggct gtataaacta
    15721 aagtgcctgc cgttgcggga gctcctagta aagaaaaaag taaaacaacc atggcggacc
    15781 tttgagcatc agtattctgt gtaaatagcg gcaagagact aggacctagc agataggtca
    15841 aggctcccat aaccagcatc atgagggtgg ataaaataaa agcttctcta ataatatacc
    15901 ttatctcagt taccttaccg ccccccagtt gcctagccac tagaataatt gttgccgttg
    15961 ccatggctaa gccaggcatg taattaaact gagtcaaggt ttctccaata gcattacctg
    16021 ccaaggccgt tgtcccaaag cgaacaacaa tgccaataat taggacatct cctgctctca
    16081 tcatcaaacg ttcaccagca gcaggtagtg ataaatcaaa aataattttg tccaaaggtc
    16141 tcatcaatcg tttcacaacc tgtttaatag gaataaactg acaaagtaaa aacataccca
    16201 ctaaacgaga taaaaccgtc gcccaagcaa caccaagaag accaaatccc catacataga
    16261 tagacaaggc agaaaaaatg gcattgagta cgtttattaa taagctcact tgcataggaa
    16321 tcttaggata cccttgagct cgaacaatag ctcctaagct agttaaaagt cctagactaa
    16381 caatcatacc gccgacaatg gatagatatt gccctccaac caatgttaca gaagcttcag
    16441 ctcctagcca ttctaacacc tgatgatgtc caacaactga taacaaccct aagccgaccg
    16501 aaaggaggag tgttacttgc aagacacctg ccatatagtt taattgtttg ttctgattat
    16561 tttctccaat acttcgagca atcagactag atacagcagc tcccagcgcg ataaagaggg
    16621 actgataaat actgataatg ttgttagcta tggagacacc agagactgct acaagtccaa
    16681 tttgagccac caggtaatta tcaaccattc ccattaacat ctgtaaaata ttttctatca
    16741 tagaaggcag agctaaagag aaaatttttc ttctattata tatcatttat gtcccctaat
    16801 aaaaaattct tgatcaaacg attttttcaa acacagtcac tggactcatt cagttatttt
    16861 ggtccattaa tgaattcata aagtctatct taatagcaga gtcccaagat acacgacact
    16921 tagtgttaca gtaacaattg accacatgcc aattcttaac atttacctaa cgtttataac
    16981 taagaagacg aagaacaaaa ggaccccaaa cgaatttttt ctattatata atatattctt
    17041 catttagcaa ctttcaatgt atcaaaatag cactctcgag tcaaaaatgg tttctgatac
    17101 tcgagagtgc tattttattt ggaaccaagt ttaagcaact taggcttatc tcatatttaa
    17161 tacatttgta tgtttagctg atgaaatcta agaccttgcg cccttggatt aaaccacgtt
    17221 ccatttcatc aaagacttct ggagcagtgt caactggaac tttttcgaca accggaacaa
    17281 ccagaccttc tgcaccaaag gcaaaggctt cttccaagtc tttacgtgtc cctacaagtg
    17341 atccgaccac tttgatacca tctaaaacag tttttacgat agacagttcc atgtattctg
    17401 atggaagacc tacggctaca actgtcccac cggcacggac actatcaatt gcttggttaa
    17461 aagcaacttt agaaacagcc gttaccacaa caccatgtgc tccaccggtt ttttcttgaa
    17521 tatagcctgg aacatcttca atttctttgc cgttaactaa aatatctgcg ccaacttctt
    17581 tagctaactc aagtttatca ttattaatgt ccactgcgac aacatgtgca ttaaagactt
    17641 ttttagcgta ttgaacagcc agatttccaa ggccacctgc cccaaagata acaatccatt
    17701 ggcctggagc agcacctgcc tctttaatag ctttataagt tgttacacca gcacaagtaa
    17761 tggaagaagc ttgagccggg tctaaacctt ctggaacttt aactgcataa tcagctgtta
    17821 ctactgcata ttcactcatt cctccatcaa cactgtatcc agcattttta acactacgac
    17881 aaagtgtctc acgtcctgta gtacagtatt cgcaatgacc acatccttca aagaaccatg
    17941 caatagagac acgatcacca acctttaagg atgttacgcc ttctccaatt tcttctacta
    18001 taccaatacc ttcgtgcccc aaaatacgtc ctggtacctg accaaaatcg ccatgtgcaa
    18061 cgtgtaaatc agtatgacaa acgccacagt attctacttt aacaagcgct tcaccatgac
    18121 caacatttgg aagatcatgc tcaacaactt cgacacctgt actagcttga ttaacaacta
    18181 cggctttcat atcagtcctc catctaataa cgacttattt gtaatcggtt acaaataaat
    18241 tgtacatctt ttcacaatta aagtcaacag aaacctgaaa agtttctgaa acttaactgg
    18301 ttaatcttat agctgtttgc ctatctgcaa aatacttaac tacattttct gacactggat
    18361 ataaatgata tatttagaaa ataaaatgat ttcttatgac tttaatcata agaaatcatt
    18421 tcctggtatc aacattaaaa atcacttacg tcgtcctgga cgttcagcat aaccatagta
    18481 agcatcttca ataatttctt gcatgtgatc aaccattgga agacgtgggt tagcaggtga
    18541 acattggtct tcataagcaa ggtaagccaa ttcacgagaa tgttctttcc attcattttc
    18601 gtcgatacct tgagctttga agttcatttg aataccaact cggcaaccca gatcataaac
    18661 agctttcgca taagattcca ctgcttcttc tggtgtagaa gctggaagcc ctaacaattt
    18721 cgcaatgtct tggtattttt catccgcacg gtagtaatta tacttaggcc atgtcgccgt
    18781 tttagctgga cgagttccat tatagcggat aacatacggc aaaagaattg catttgtacg
    18841 tccgtgtacg gtatggaatt gtgcgccaat tttatgtgcc attgagtgag aaatacctaa
    18901 gaatgcattg gcgaaagcca tacctgccat agttgacgca ttgtgcatct tttcacgtgc
    18961 ttcaaaatcg gctgttttaa ctgatttttc aagattatca aaaacaattt taatggcttg
    19021 aagagctaaa ccatccgtaa agtcattggc catttgtgac acgtaagctt cggttgcgtg
    19081 tgtcaaaacg tccataccag tatcagcagc aataaagcct ggaacagtca ataccaaagc
    19141 tgggtcaaca atggctacag ttggtgttaa tgagtaatca gcgattggat atttacgatt
    19201 atttgctttg tcagagataa cggcaaatgg tgtcacctct gaacctgttc cagaagttgt
    19261 tgggatagca acaaattttg ttttcttacc aagttctggg aatttgaacg cacgtttgcg
    19321 gatatccatg aatttttgaa cgaggtcatg gaaatcaact tctggttgct cgtagaagag
    19381 ccacataact ttggctgcat ccataggaga tcccccgcca agggcaataa tggtgtctgg
    19441 tttgaaggta cgcattaatt cagtaccttt cataacagtt gtaatgtctg gatctggttc
    19501 tacttcagca aagatctgat aaacaacttt attgcgacga agatcaagtt gttcaatgat
    19561 acgatccaag aaaccaagct caaccatagc gtgatctgta acaatcatga cacgttcaac
    19621 gtcacgacat ttttgcaaat attgaattga atcacgttcg aagtatgttt ttgaaggaac
    19681 tttaaaccat tgcatattat ttctacgtct tcctactttt ttgatgttca agagatttat
    19741 agcactaacg ttatcaccaa ctgcgttacg tccatacgaa ccacaaccaa gagtcaatga
    19801 tggcaagaaa gcattgtaaa catccccgat accaccaaat gtagaaggtg aattccagat
    19861 gacgcggata gcacggattc tagttccaaa ttcttttgcc aactctgcat ctgctgtatg
    19921 aatggcagct gagtgaccga gtccattaaa ttcaaccatt tgacgagctt tttcaacacc
    19981 gtcttcacgt gattcagatt tcaaaacagc aatgactgga gataattttt cacgtgttaa
    20041 aggctcattt tctgaaactt ctttacattc tgcagcaaga atattggttc cttctggaac
    20101 agtaaatcca gcttgttctg caatccaagt agctggtttt cctacaatat ttggatttaa
    20161 ttttgcacca gcacagtttt tgctatttgc tttggcacca aaacagaatt cttcaagaag
    20221 ggctttttct tttttattta caaaataagt atggtaagat ttgaactctg ctacaaaatc
    20281 gtcatagatt tctttatcga tgataacagc ttgttcagag gcacagacca taccattatc
    20341 aaatgattta gacatcacaa tatcatgagc ggcttgacgg atattagctg atttttcaac
    20401 ataagctgga acgttaccag cacctacccc aagggcaggt ttcccacatg agtatgccgc
    20461 tttaaccatc gcatttccac ctgtcgcaag gatagtggca atgccgtcat gattcatcaa
    20521 agcatttgtt gcttcaagtg atggtgtttc aacccactgc acacaatttt caggagcacc
    20581 agcggcaata gcagcatcac gaacaatacg agcggcatgc gctgatgatt cttgtgctga
    20641 tgggtgaaag gcaaaaataa ttggattacg tgtttttaac gagatcaaag atttaaagat
    20701 agctgttgaa gttgggttag ttgttggtgt aatcccacag ataacaccca ccggctcagc
    20761 aattaatgtc aaacctgtca catcatcttc ttcaataatt cctactgttt tttgatggcg
    20821 catgttatta accacatgct cacaagcaaa tagatttttt gttgctttat cttcaaagac
    20881 accgcgacct gtttcttcat aagcatgttt agcaagttca ccgtgagcat ccaaagcagc
    20941 aacagaagct ttggctacga tataatcaac ttgttcttgg tcaagcttac gcatctcttc
    21001 aagagcagct aaaccttttt gaacaagagc atcaattgtc acagaaacag aggttgtttc
    21061 aacagtatta tgtccttcag tcatttgaga ctcctttatc ttgttaaaag tttcacaatt
    21121 ttgtttacaa aattattata gcactttttg aaattttgta aatcatttca caataaattt
    21181 gtgaagtttt tttcaaatta tttctgaaat cctatggtat caatattctt cattaactct
    21241 ttttcagaaa tatatctaaa aaaagccgtt ttatttctga aatcgctttc ttttaaaaac
    21301 ctcaaagtac tactatttta ccatcatcat ttgttcacaa gaaaaagcca gagcacaatg
    21361 gatctgactt atctcttaga catttcttaa ctttatttag ctggtttttt agctgacttc
    21421 tcaacagcag tctggattgt ggagacatat agttctgctc cttgactatc ttcactataa
    21481 tgaacaccgt cactgccata ccaaatatct ggatgttcaa cagcaacttt ataccagtct
    21541 gcaatagtga catacttgta ttttttggac aatttaagac catagtctct agcttctgca
    21601 acctgactgg catttttggc attatatgga gaaaccaaaa ctaaacgatg accatttggc
    21661 aatgcctcaa taaaagactg aactgcttga gaatagttgt caagcgagtt aacacctact
    21721 gctaagacaa cagtcttaga tagactctta gatttaattt ggttattaaa taaatcgaaa
    21781 gcttctttaa aattccgact gaccgctgca tccagttgcg cttgtggcat aatttttgag
    21841 aatgctgcac tagagcgtaa tgctacagaa tcaccaataa tacctacgtc actcaaagca
    21901 ttagcgtctc ctgcagctag tgtatgtgtc cgctccatat tactttgagc ctgttgtagg
    21961 gaactaacca acaattgttt ctcaaactta ccaactttag gcgctatcat acaagttcct
    22021 aaagttaata atgctaaggc gagagtccct cctacaatcc acttatagta aggagaacag
    22081 tcaatttcta aaccaagcag gtttggtttt ctcccttgga ccaaaggttc tacaatatag
    22141 taagacacag tggcaaacaa gattgaaaag aaaactgtta gaataactgc caaaatatga
    22201 gacattaatt ggctaaaaat aatgtaaaat ggccaatgga agaggtaaat cccatagcta
    22261 atatcagcta tataagtgat agctttaggc tcttgtacat ctggtgtttg ctcatgaaga
    22321 acacgcgctg cataaatcat aattgatgcg aataagctag ccaaagcaaa gccaaataga
    22381 taggtcgtga tatggttaaa atccaatgtg accgttagca caagcaaaag tgtaaatgct
    22441 ccaaccatag cagcaaggac atactggcgc ggccataaac gtacattttt ttgaaatcga
    22501 acagtagtct cgttaatacc tgttatcgtg gcaaacatcg cccctaaaaa gaagggaaaa
    22561 ctgtgagaaa gactcgaaaa ataaatcagg gaaaaatttg aggtcataaa agatcttata
    22621 aacatgctca agaaactaat agcaaatatt cccaaagaaa tcaaaaacag tagtcctctc
    22681 agttgtttct gagtctcctt gcgtctagct aataaccaaa caaatacacc ccaaaacaag
    22741 taaaaatgaa cttcaatagc taagctccac gtatgaacaa agagatgtgg aataaattgg
    22801 ctttcataac tacttcccgt caatatttca taaatattag tggtaaatcc taaaacagct
    22861 gtaatctgac ttcctatatt agcaatgaaa tccttcttaa ttaaaaacgt aaaaggaatg
    22921 gtcaataaaa tcattaacac caaaggagga acaattcgat aaaagcgtct ttttaaaaaa
    22981 ccgataatat cgatagactc tttttttgtg tattcatcaa tcaagagggc tgtgattaga
    23041 tatcctgaaa aggtgaagaa aatgtcaaca ccaataaaac ctcctggaaa tacattttta
    23101 aaaaagtggt agagtaaaac cagtaaaagt cctgttaccc taacaaacga aaaccatttt
    23161 attctcattt ttgaaatatc ccctgtttaa aaatcccttt atagacttta ttattctttg
    23221 cttttaacac acctttgcca tttgcttgtc ctttatgaaa ttccccgtta tataaccagc
    23281 ccgtcttagc agtaaaagtt ccttttcctt caaataggcc attcttaaaa gtaccctcat
    23341 agatatcacc gttggggtag acaagcttcc cttctccatt cattttatgg ttaatcacgt
    23401 aaccagtata gtgtatacgc cctttatcat aggtcaaact tgtcttattc gaaattctta
    23461 cgctaaaaac agaaattgca caaaccaata tgacaatcac cgaaacaatt tccaacttcg
    23521 cccgtgtaat actccatttt tttactatat cttgcatatc tatcatttct tttactcaac
    23581 ttctaatatc aatactcttt actcattaaa cgagaaagcc aatcctggca tccttttcta
    23641 actaattggt aggtttcttc aaaatcgtta gtgtaccaag gatcaggaac acccccttct
    23701 ctaaacagat agattttaga gtcccattga tgttgcgaca tctctttgag atttttcaca
    23761 ttatcgctat ccataccaat aatatagtca aaggtgttaa aatcagttat agttatctgc
    23821 ttggagcatt tagttatatc ataattaatt tggtaagttt ttaatatgga ctgtgtacct
    23881 gagtgaatag ggtttccatg ctcccaatca gaagtagcac gactttctat catcataaca
    23941 tcacttgata ctattgactt cattacaaac tcagccatgg ggcttcgaca aatattaccc
    24001 agacagacaa aacatacttt tttcattaaa tactcctttg aattattata tcaaatttct
    24061 aactgatatc ggagaaatca atctaataat tttctgtcaa cttatcaaaa aatccgaaca
    24121 caaaaagcag gttcagatta attttcttag atttgaaaaa tcattaagtg ttttgatagg
    24181 gatagccaag atgacgataa gccttttgag tcgctacacg acctgttcgt gttcgcatga
    24241 gaaagccttt ttgaattaag tagggttcat acatctcttc aactgtatta cgctcctcgg
    24301 caatattcac agataatgtc cccaaaccaa ctggccctcc ctgatacatc tcaatcatcg
    24361 ttcgtaaaat tttctgatca atgtaatcga gaccttcgcg gtcaacatct aacatagtca
    24421 aagctctatc agttatttga gccgttatga tgccatcacc aataatttga gcatagtcac
    24481 gcactcgttt tagtaatcga ttagcaatgc ggggcgttcc tctactgcga caagctaatt
    24541 tccgtgcagc ttcatggtct atttttattt caaaaatagt agctgtacgc tccacaatct
    24601 ctgtcaaatc cttttcttga tagtattcca tgtgaccagt gataccaaaa cgagctctga
    24661 ggggattaga taacatgcct gcacgtgtag tagcaccaat caatgtaaat ggaggcagat
    24721 ccaaatgaat actacgacta gtatctccag cgccaatcat aatatcaata tagaagtctt
    24781 ccatagcact atatagcact tcctcgacag acattggcat gcggtgaatt tcatcaataa
    24841 acaaaatatc ccctggttct aattcattta aaatagctac caaatctcca gctttctcaa
    24901 cggcaggacc cgaagtttgc tttaaattaa ctcccaattc attggcaatc acaaatgcca
    24961 ttgttgtttt tcccagtcct ggaggtccaa ataataaaac atggtctaaa gattcgtcac
    25021 gtcttttagc tgcttctata aaaatagcga attgttcctt aactttatct tgtccaatat
    25081 attcgtgtag atattgaggg cgaagagtcc gatccgaaaa ctcctcattt cccattacat
    25141 tattgtctaa aattctagcc ataggactat tataacataa agtaaaagag agttaaccaa
    25201 gattccacc
//
LOCUS       DAUCWO010000020        25188 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUCWO010000020 DAUCWO010000000
VERSION     DAUCWO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25188
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.020"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            151..312
                     /gene="sagA"
                     /locus_tag="VGY20_000783"
     CDS             151..312
                     /gene="sagA"
                     /locus_tag="VGY20_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ4273652.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            534..1484
                     /gene="sagB"
                     /locus_tag="VGY20_000784"
     CDS             534..1484
                     /gene="sagB"
                     /locus_tag="VGY20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ4273653.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1481..2539
                     /locus_tag="VGY20_000785"
     CDS             1481..2539
                     /locus_tag="VGY20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ4273654.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIVQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2559..3917
                     /locus_tag="VGY20_000786"
     CDS             2559..3917
                     /locus_tag="VGY20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ4273655.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3892..4563
                     /locus_tag="VGY20_000787"
     CDS             3892..4563
                     /locus_tag="VGY20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ4273656.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPKITLSLESIDSKTFFMGLVFCVFIVLIELVFLHGLRCWQKK
                     EWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4560..5243
                     /locus_tag="VGY20_000788"
     CDS             4560..5243
                     /locus_tag="VGY20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ4273657.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLMALSYQDFLDV
                     LLSLFQFVVIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5266..6189
                     /locus_tag="VGY20_000789"
     CDS             5266..6189
                     /locus_tag="VGY20_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273658.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6198..7325
                     /locus_tag="VGY20_000790"
     CDS             6198..7325
                     /locus_tag="VGY20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273659.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIRLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7322..8440
                     /locus_tag="VGY20_000791"
     CDS             7322..8440
                     /locus_tag="VGY20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273660.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALIIFSSLLLKPTRL"
     gene            9012..11744
                     /locus_tag="VGY20_000792"
     CDS             9012..11744
                     /locus_tag="VGY20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ4273661.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNVAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11909..12024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            12022..12522
                     /locus_tag="VGY20_000793"
     CDS             12022..12522
                     /locus_tag="VGY20_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ4273662.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12716..14674
                     /gene="ligA"
                     /locus_tag="VGY20_000794"
     CDS             12716..14674
                     /gene="ligA"
                     /locus_tag="VGY20_000794"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ4273663.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRELATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKENFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRKL"
     gene            14688..15710
                     /locus_tag="VGY20_000795"
     CDS             14688..15710
                     /locus_tag="VGY20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ4273664.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDEALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            16102..16299
                     /locus_tag="VGY20_000796"
     CDS             16102..16299
                     /locus_tag="VGY20_000796"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ4273665.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16334..17050
                     /gene="atpB"
                     /locus_tag="VGY20_000797"
     CDS             16334..17050
                     /gene="atpB"
                     /locus_tag="VGY20_000797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ4273666.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            17068..17562
                     /gene="atpF"
                     /locus_tag="VGY20_000798"
     CDS             17068..17562
                     /gene="atpF"
                     /locus_tag="VGY20_000798"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ4273667.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17562..18098
                     /locus_tag="VGY20_000799"
     CDS             17562..18098
                     /locus_tag="VGY20_000799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ4273668.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            18114..19622
                     /locus_tag="VGY20_000800"
     CDS             18114..19622
                     /locus_tag="VGY20_000800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ4273669.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19638..20513
                     /locus_tag="VGY20_000801"
     CDS             19638..20513
                     /locus_tag="VGY20_000801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ4273670.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20675..22081
                     /gene="atpD"
                     /locus_tag="VGY20_000802"
     CDS             20675..22081
                     /gene="atpD"
                     /locus_tag="VGY20_000802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ4273671.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            22094..22510
                     /locus_tag="VGY20_000803"
     CDS             22094..22510
                     /locus_tag="VGY20_000803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ4273672.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22776..23033
                     /locus_tag="VGY20_000804"
     CDS             22776..23033
                     /locus_tag="VGY20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ4273673.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            23098..24369
                     /gene="murA"
                     /locus_tag="VGY20_000805"
     CDS             23098..24369
                     /gene="murA"
                     /locus_tag="VGY20_000805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ4273674.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24373..24561
                     /locus_tag="VGY20_000806"
     CDS             24373..24561
                     /locus_tag="VGY20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ4273675.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            24597..25188
                     /locus_tag="VGY20_000807"
                     /pseudo
     CDS             24597..25188
                     /locus_tag="VGY20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         7812 a         4174 c         5162 g         8040 t
ORIGIN      
        1 aaaagtgata agaactagat agttgttgtg ttacaacagt acaattgagc tagccttgtc
       61 cttgttgtgt taactttatt tttaaaataa ggttaaaaat aaacgactcg tgttcttatc
      121 agttacttat tagataagga ggtaaacctt atgttaaaat ttacttcaaa tattttagct
      181 actagtgtag ctgaaacaac tcaagttgct cctggaggct gctgttgctg ctgtactact
      241 tgttgcttct caattgctac tggaagtggt aattctcaag gtggtagcgg aagttatacg
      301 ccaggtaaat aatctattta gcatctctat gtgatagtga tattaaggta atgagtttgc
      361 tagcaactaa gttatctttg gtaacttctg attatagatg aatggcatag aggtgttaga
      421 aaacatgaga caaaagtaat cttcttgatg ctaacgtctc aggtaattag caggtactag
      481 acagtacctg ctaattacta tatgtttagt aaaatgagat aggaaagata gttatgtcat
      541 tttttacaaa ggaacaacaa cctaaagaga attgtccgcc aataactgtt gaaaaagcaa
      601 ggcaattgtt tgaatttaat acaaaccact tgtccttatc ggattaccat catcaaacgg
      661 tgctaaaaac gtcaaagcag ctagttgctc aacatttaat gcctaatgca accgataatc
      721 ttagtcaaca ttttttgatg aactataaag ctaataataa ttatttaggc ttccaagcta
      781 gtattgtcga cttttttaca gattctgccg ttgctaattt ttcaagtagt tacgtttatg
      841 aaagtcagga aaagataatt cgtttaccaa aacctaccaa gatatcaact gctctgtcga
      901 catgtattat aaaacgaaga agtcatcgtc aattttcaga tagacaaatg cctcttcaag
      961 atttatcaaa cattctttat tatgcatgtg gtgttagttc acaagcatca attagagatg
     1021 gagcatcaga taagattaca ctcagaaact gtgcttcagg tggaggttta taccctattc
     1081 atttagtttt ttatgctaga aacatcagta aattaataga tggtttctat gaatatctac
     1141 cctatcagca tgcactaagg tgttatcggc atagctctga ggaaaacgtt agagattttg
     1201 cggaatacgg tgctattaat gctgaaaatt gtaatattat tattatttat gtctaccatt
     1261 acatgaaaaa tacacgtaaa tatgggaatc aggcgactgc ctatgctttt attgaatcag
     1321 gagaaatagc ccagaatatt caattgactg caactgcctt aacttatgga agtattgata
     1381 ttggtggtta taataaggaa tatctccaag aattattaga tttagatggg ctaggagagc
     1441 atgtgattca catgacactc gtaggaacta aggagtctca atgaaatatc aacttaatag
     1501 taatgttcgt gtcgtgacat ttcaagatac attttgtttt agaaaaggaa tctgggactt
     1561 taatgaagcc attctagatt tgacacaaga acctcaaaat ttaaagatag tttatcaaga
     1621 aatagtttca cagttggtta agggagtagc cattgatact gaaacttatg aaaatacttt
     1681 agaacctgag acgttcgcta atttaatgga agtcatttca ggcttgtatt acaatgatat
     1741 gctgatgctt gaggatgact acaatcttga agaaaatgtc atgaaaattt taatgggtaa
     1801 tttccgtttt atggcgcaag caggtcatat ggtaagcaat gctcctgttt tgtttatcag
     1861 tgattcgact tatgttaatg aatctgctga gttgctagct gaacaactgc atttacaatt
     1921 gcaagtggct agtgatgact taaaaatgtt aattcagcag acagatgtta gttcgcgttt
     1981 agatgctttg gagcatcgcc gtaacatgaa ttgtttatca gaagccttgt gcgattacca
     2041 aagtattatt atttgtcaag aacgcttgaa tattatgatg ttgcgacact tgaatgaagt
     2101 tagcgtagct atgaaaaaac aattagtgat tggttttgta gatggaccat tcttgcacac
     2161 ttgtactttg aatcctcccc atagtgctga ctttgatagc ttagaaagaa gagttttggc
     2221 acgtttacaa gactcaactc tctatcaaca ttttgctaat caggttttac cagccacaca
     2281 agatgtgagc caggcctatt taccattgtt aaatgtttta atgaatttag tcgttagcga
     2341 agctttcatt attgttcaaa caggtagttc taaatttgag ggacgtttat taagcattta
     2401 cttaccgacg cttgagattc aggtccaaga tattttgaaa atgtctaatt cacagacaca
     2461 aggagctttg gcaaaattaa agtatgagga tcagcagata tcaacacgtg agattgttaa
     2521 aaaactcctt gacgagtaac ctataaggag aatctattat gttatactat tatccttctt
     2581 ttaatcatat ttttgatgaa ttaaaaagtt taagtggcaa tcgaacaggt atcttaaatc
     2641 aatctcaagt tccagtttgc aaccatcctc acgatgttta cttaaaaagt attactggcc
     2701 aaatgccaga ttatcataaa caatttatag gtgagttgag tcaagttagt tatcacatta
     2761 ttggttacgg cagttactac gaagaagcac ttatcaagta tttaggagaa agtattgaac
     2821 gctatgctac tgtaattgcc ggagacttac tatcagatcg gattgtctat gcttcttata
     2881 atgagttaaa actgttacat aaagtgatgc ctttagaata cttgcaagtg tttacccaag
     2941 aacagatcgc attatcttgt gacttgcaaa tgatgatgtg cgataaaatg gtaactgaga
     3001 acgatgtatt gggctgggtt aaatgtccca tgttctttga ggacgcagag atgtatgttc
     3061 cagctcaaat gctttgtgtt gggtataaaa caaatgagac tgtaggagaa agacggataa
     3121 taccaggatt ttctacggga acagcttctc acaaaacgtt agaagcggct atgtgcaaca
     3181 gtttgattga atacatccag atcgattcaa tgatgcttag ctggtatact aagaagcctt
     3241 gccccaagat tattgttgat gatcctgata ttgaagtgat tttagaagaa gctagactgg
     3301 gaaaagacag tctatacgac attattccta tcgatatgac tgtaggagaa gataatcctc
     3361 tttatacgtt tggaatcatt ctcaaaaata aatatgatga agggccttac ctcttgtttg
     3421 gagtgcaggc tgggttagat cctaaacatg ctttgctaag agggatcatg gaagcttcag
     3481 ctattagcta tagttactac tataatcttt tatatcaaaa agcttccctt gcaaatattg
     3541 aatgcgaaga acctttgttt ttagatttag atagtaatgt cttctattat gcacatccca
     3601 aggaccaaga ccataaatgg aaggcctttg aacctttgat ttcaggagaa gttctgttaa
     3661 gtgatttgga agatcattct ggtaaagata aaaaagaaga tttgaagact ttattagctt
     3721 atgctaagaa agtcagtcct aatgctgtct ttttagatat tacacctcct gaagctctag
     3781 aaaaaggctg gtacgtcacg cgagttctga tgccagaact tcttgaaatg tgtataccag
     3841 catttccttt tgctaatcat ccacgaatga gacagtttgg aggtgtaaca aatgcctttg
     3901 tccatccaat gccttaatct ttgttttttg ttggtaactt tttgtccttc aattcctatg
     3961 caagctattt ttggaaaaga agatagtggt tatgcattta atttaattgg ttttctaaga
     4021 gcaactctga tttatgacat tttggctttg gtaagtattt atgttttgtc acctaaaata
     4081 accttgtctc ttgaaagtat tgatagtaag acgttcttta tgggacttgt attttgcgtc
     4141 tttatagtac tgattgaact cgttttctta catggtttac gttgttggca aaaaaaggaa
     4201 tggcttcctg caactttctc gtttgtagga acgacaaatg attggtctaa gattggctat
     4261 cctttattac tagctttatt tgaagagacg atttatcgtt ttttgtggtt taatatatta
     4321 gcttttcaat ggcatttacc aacaattatt gttttaattg taactagttt ttgctatgct
     4381 ttaaatcact tattgatggg gaaatccatt ttttatgcta agttggtaac aggcatcatt
     4441 tatggtagta tttacatgct gactagtcaa ttgtggttgg ttgtgataat gcatgtagga
     4501 ggtaacttgc tagttgagtg tcttagccat cttcaaacaa aaaagaagaa ggaggtgaca
     4561 tgatgctatt ggttttgctg tcgtttttag gtctaactac tctttggttg accagttatc
     4621 gaagacgttg tttggctaga tggcatcttc aatggttaat ggccttatcc tatcaagatt
     4681 ttttagatgt cttactgagt ctgtttcaat ttgtcgttat tatacttgtt ttgttctttt
     4741 actcagctac tattaatcta ggagaggttc tgaccttttt gacacaaact agctggcatt
     4801 ggcagattct ttgttattta gtgttgtatc ttatggccat catagagatg acgcttttag
     4861 ttttaattct tatctttgat gttttgctcc agaaagatag tcgattgctc tttaaaaaaa
     4921 taacttggct gccttttaga caagataaac cagtcttaag tatacttttg ctaggcatag
     4981 ttattctagt agacagtctc ttttatttgg gcttgcttat attattagga gaacagtcgc
     5041 taacaagttt agcaacgctt attttaggct atgggatagt caaggcttgt cgctattcag
     5101 gttggttaaa tgtactatta gctttttgtc ttttcactat agttggacta tgggcagtta
     5161 cggcaacttt attatatggt tggctagtag gtgcgatgat attaacggtg acttatttga
     5221 tgattagttg caaagagtat tagcaatatt aaaaaggagt gatatatgag ttttgtacaa
     5281 ttaacaaatg ttgtcaagtc ctacaaaaac ggcaagaaag ctgtcaatga tgtttccttg
     5341 tctattgaag caggtaatat ttatggtttg ttaggaccaa atggtgctgg taagtctacc
     5401 ttgattaatc ttatcttagg cttgatccct ttgagttccg gtaaaattac tgttttaggg
     5461 caatcccaaa agactattcg aaaaataagt tcgcagatag gttatgttcc tcaagacatt
     5521 gctgtttatc cagacctaac tgcttatgaa aatgtagaac tatttgggtc actttatggc
     5581 ttaaagggag ctcagcttaa aaaacaagtt ctaaaaagtt tagaatttgt gggactacac
     5641 tcccaagcta agcagtttcc aagtcaattc tcaggaggaa tgaagagacg gttaaatatc
     5701 gcttgtgcgc tagttcattc acctaaatta atcatttttg acgaaccgac tgtagggatt
     5761 gatcctcaat cacgtaatca tattttagag tcgattcgtt tgctaaacaa agaaggtgcc
     5821 acagttattt atacgaccca ttatatggaa gaagtagagg ctctttgtga ttatattttt
     5881 attatggatc atggtcaagt tattgaagaa ggacctaaat ttgaactgga aaaacgttac
     5941 gttgcaaatc tagcaaacca gattattgta actctaacag attcacgtca tttggaactg
     6001 gcagataagc ctgattggtc tttgatagaa gatggagaaa aactcatgtt gaagattgat
     6061 aatagtgata tgacatcagt tgttcatcag ctcacacagg ccaatattac ttttagcgag
     6121 attagacata accatttgaa tttagaagaa attttcttac acttaacagg taagaagtta
     6181 cgagattaga ggttactatg gttttatttc atttaatcaa aaaagaaagt ttacagattt
     6241 ttagaaaccg aacagcctta ttgatgatgg tgatttttcc aattttgatg atcgttattt
     6301 taagttttgc ctttaaatca agttttaata ctgcgacaac agtccctaaa ttgaccattc
     6361 gttaccaatt agagggtgaa aaaacggatt accagaaaaa ttttcttgct tttttaaaag
     6421 ttttaaacca aaaacttcat ttagaggcta aacctagtaa ttctcttgaa aaagatcgac
     6481 aaagggtcag tgaaggagcc ttaacggctg ttttagaagt gaagaagaat cagaccatta
     6541 aggttattac taataatatt aatcagcaaa atgcagattt aatcaatatg ctagtaaaaa
     6601 attatgtaga taatgctaaa acttatgact cgatagcagc tctttatcct caacaattaa
     6661 atcatatcag aaagcgaagt gtggactatg ttaaggtcag ttcaatacag acaagtaaag
     6721 gaatgacatc agctgattat tatgctattt ccatgtttac catgattact ttttatagta
     6781 tgatgtctgc gatgaacctt gttttgtcag atcgtcaaca acggattaca aatcgtattc
     6841 gcttaacagg agtttctcca agtcttttag tctttgggaa attaataggt gctatgttag
     6901 caacaactgt tcaattgagt cttttataca tttttacaag gtttgtttta cgagttaatt
     6961 ggggcactaa tgagtggatg cttattggta taacagcctc cttagtctat ctctctgtag
     7021 ctataggtat cgggctaggc ataagcatta aaaatgaggc ttttttaacg gttgcatcca
     7081 atactattat tcctatattc gcctttttag gaggcagtta cgttccgttg acaacattac
     7141 acagctctat tattaatcaa ttgtcgaata tatctcctat taaatgggtt aatgatagtt
     7201 tgttctatct tatttttggt ggtcaatata atccgattcc tgtaactttg atcgttaata
     7261 ttagtatcgg aacaattttt ataatattgg cattaatagg tatgagaaag caggtgacga
     7321 catgatttgt tttataaaaa ctctatttgt taagataaag cgcaaaaaaa caagttatgt
     7381 gacattcttt ttaatgccta ttttaacgac acttttggct ttatctttga gtttttcaaa
     7441 taataatcag gctaaaattg ggatattaga taaagataat agtcagatat ctaaacaatt
     7501 tattgctcaa ctaaagcaaa acaaaaagta cgacattttt accaaaatca aaaaagagca
     7561 tattgatcac taccttcaag ataaatctct agaagcggta ttgactattg ataaagggtt
     7621 ctcagataaa gtgttgcaag gaaaatctca aaaactcaat atacgttcga ttgctaatag
     7681 cgaaattaca gagtgggtaa aggcacagac caattatcta ttggaaaact ataatattat
     7741 aggggatgtg gctctaggta acgaagatac ttttaataga attctccaaa aaaaccaaca
     7801 gttaaactat gatgttaagc aagtaacttt gacagaccga tcacgtagca aagcagtatc
     7861 ttccacaacg acaggatttt tgcttattct aatgttaggt agtacgagcg tgatttacag
     7921 tggtattttg gcagataaat ctagtcagtt ataccatcga ttaatgttat cgaacttatc
     7981 tcgttttcgc tatatgttga gttacgtctg tgtaggattt gttgctttta cgattcaaat
     8041 tgtcattatg ctgagtttgt taaaagtatt taatattagt ttttttgttc cgacatcact
     8101 tcttttgatt atttttttcc tttttagttt attagcgata ggctttggcc tgctgattgg
     8161 agctatcaca caaaattccc aacaaagtag ccagttagct aatcttattg taatgccgac
     8221 ttcaatgttg gctggctgtt tatggccatt gtctattacg ccttcttata tgcaagcaat
     8281 tggtaagtta ttaccacaaa attgggtttt atcagccata gctatttttc aaagtggagg
     8341 gaccttatct caggcttggc catacttgtt agctctaatg ggaacagcac tagctttgat
     8401 tattttttca agtttattat taaaacctac cagactataa ttagtctttt gttaaaaaat
     8461 actttcctaa ttagctaact accaggtagc ccttttattg gcttatatgc aataaaaggg
     8521 ttgctttttg cttatatttt ctctagtgca cttatctttt gttaaaagcg attgcgatgt
     8581 tactgttgtt actgtgtttt ttagagagca tattatcaaa agcgacctct ctagaaagtt
     8641 cgtttttgtt gataaatgag ttgtcaaagt attttaaaaa aacgttttca ataaacataa
     8701 aaactaatgt tattaaaaaa aggtgtaaaa acgtatttat tttatgaaat gtttttgtta
     8761 caataagcat gtatcgaatg ctataaagct gtcttgttcg ctatcagggg tgcttgtgat
     8821 gttatgtcag atcaaacaag tctttaattg ggtgacttaa cagattaaaa gaaaagatct
     8881 aatcgtgtta cttgatagta taattaatgc aaacagaaag tgtatcccaa ttcatagtct
     8941 caggtggagg ttaatattca aaaataaaat cttgttgttg tcttaaacta atcacattaa
     9001 aaggagatat catgattaac aagaaatata taatacctgt ttcattgttg acactagcta
     9061 ttacgcttac tagtgttgaa gaagttactt cacgccaaaa tttgacttat gccaatgaaa
     9121 tcgtaacaca aaggccaaag agagaatctg ttattagtga taaatcgaat tttcccgtca
     9181 tatcacctta cctagcaagt gtggattttg gtgagagaaa aacacctttg ccaacacctg
     9241 ataaaggagt aaaagtaact actgaacagt ctattgctca agtaagaaag gggcctgaag
     9301 aaagaaccta tactgttact ggcaagatta cgagtgtgat caatggctgg ggaggctatg
     9361 gcttttatat tcaagatagt gaaggtattg gactttatgt ttatcctcaa aaagatttag
     9421 gatacagtaa gggagatatt gttcaattaa caggtacact tactcgcttt aaaggtgatt
     9481 tacaactcca acaggtgact gcacacaaaa agttagagtt atcttttccg acttctgtta
     9541 aagaagcagt aatatcagaa ttagaaacaa caacaccctc aacattagtt aagttatctc
     9601 acgtgacagt tggagaatta tcaactgatc aatataacaa cacatctttc cttgtaaggg
     9661 atgatagtgg taaaagtata gttgttcata tagatcatcg tacaggggtt aaaggggctg
     9721 atgttgttac taaaataagt cagggtgatt tgattaacct cacagccata ttgtctattg
     9781 ttgatggtca gttacaatta agaccgtttt ctcttgaaca attggaagtg gttaaaaagg
     9841 tcacaagctc aaatagtgat gcttcatctc gtaatattgt gaaaataggc gagattcaag
     9901 gagctagtca tacgtcgcca cttctcaaaa aagcggtcac cgtagaacag gttgttgtca
     9961 cttatttaga cgattccact catttttatg ttcaagatct taatggtgat ggtgatttag
    10021 cgacttcaga tggtattcgt gtttttgcta aaaacgctaa ggttcaagtc ggcgatgttt
    10081 tgaccatttc aggtgaagtg gaagaattct ttggtcgtgg ttatgaggaa cgtaagcaga
    10141 ctgaccttac catcacccaa attgtggcta aagcagtgac caaaacaggg acagctcaag
    10201 ttccatcacc gcttgtttta gggaaagacc gtatcgcgcc agccaatatt attgataatg
    10261 atggcttgcg tgtgtttgat ccagaagaag acgctattga ttattgggaa tcaatggaag
    10321 gcatgttagt ggcggttgat gatgctaaaa tccttggtcc aatgaaaaat aaagaaattt
    10381 atgtcttacc tggctctagt acaagaccgt taaataattc aggtggagta ttacttccag
    10441 ctaattctta taacacagat gtgattcctg ttcttttcaa aaaaggcaaa caaattatta
    10501 aagcaggaga ctcttacaaa ggaagattag ctgggccagt atcttatagc tatggtaatt
    10561 acaaggtctt tgttgatgac agcaaaaaca tgccaagttt aatggatggt catctaaaac
    10621 ctgaaaaaac aaacttgcaa aaagatctta gtaagttaag cattgcttct tacaatattg
    10681 aaaacttctc agccaatcct tcttcaacta aagatgagaa ggtcaaacgg attgccgaat
    10741 cctttattca tgatctgaat gctccagaca tcattggatt aattgaagtc caagataata
    10801 atgggccgac tgatgatggg acaacggatg cgacacaaag cgcgcaacgc ctcattgatg
    10861 ctattaaaaa actaggcggc ccaacttatc gttatgttga tattgctcca gaaaataatg
    10921 ttgacggagg tcaaccaggt ggtaatattc gaacaggatt cctctatcaa ccagagcgcg
    10981 tcagcctttc tgataagcca aaaggcggtg ctcgtgatgc tctaacttgg gttaatggag
    11041 aattaaacct tagtgttggt cgaattgatc caactaacgt cgcttggaaa gatgttcgta
    11101 aatcactagc agcagaattt atcttccaag gtcgtaaagt cgttgttgtt gcaaatcatt
    11161 tgaactctaa gcgtggggat aatgctcttt atggtcgtgt gcaaccagtc acttttaaat
    11221 ctgagcaaag acgtcacgtc ttggctaata tgctagcaca atttgcgaaa gaaggggcaa
    11281 aacaccaagc taatattgtg atgctaggtg actttaatga ttttgaattc acaaagacga
    11341 ttcaattaat cgaagaaggt gatatggtta acttggtgag ccgacatgat atttcagatc
    11401 ggtattctta ttttcaccaa ggcaataatc agacccttga taatatatta gtttcacgcc
    11461 atttacttga tcactacgaa tttgacatgg ttcatgtgaa ttccccattt atggaagctc
    11521 acggacgcgc atcagatcat gatccattgt tacttcaatt atcattttct aaagaaaatg
    11581 ataaggcaga gtcttctaaa caaagtgtaa aagctaaaaa aacttcaaaa ggaaaactgt
    11641 tgccaaaaac aggagatagt cttgtttatg tgataacact actaggaacg gctagtttat
    11701 tagtgcctat tttattattg actaaaggca aaaaggaatc atagcgattt gaagaattgg
    11761 agttcataaa gagtcgttaa aggcttgata tacttacgtt agaccttaag ctaaaatgga
    11821 tgactatata aaaactaaga ttgataacaa aagaagataa caataagaca gctttacttg
    11881 ctaaagactt ttgtcaagct aattgtttta tggtacaatg taaccgttgc tttgcaaccc
    11941 ttggtgctta gttccctttc accaagcata ttagaaacgt agatagcttt tgagagttat
    12001 ccagacgtta aaggagaaaa aatgacaaaa ttgactgtac acgactatgt tcacattggg
    12061 ctagtagctg ccttgtatgt tgtcttaact attaccccgc ctttaaatgc gatttcttat
    12121 gggatgtatc aatttagaat atctgaaatg atgaatttct tagcttttta tcatcgcaag
    12181 tatattattg cggtgactct tggatgtatg attgctaact tttatagttt cggactgatt
    12241 gatgtctttg taggcggcgg ttcaactctt atttttgtga ccttaggtgt gattttgttt
    12301 agtaagtatc aaaaagatta tctttttaat ggcattttta ataaagcttt tgtttacttt
    12361 tctttctttt ttgcaacttc catgtttact gtagcaatcg aactttactt ctttggagct
    12421 ccatttttat taacatggtt tacaactgcg ttaggagaat tagtatctct gctcatagga
    12481 tcacttatta ttgataagtt gtctcaaaga atttcctttt agtaatgcat catactcaaa
    12541 agagttgggt caataccctt ctctttttta tgttgtttgt aatgttaaaa tcgttctaga
    12601 tagaaatatt taacacttta gctaatagta ccatatggag tggccaacga ttacttttga
    12661 tgttgttatg atatagaaat agctcatttg ttatctttta tgataaaata aaagtatgaa
    12721 aaaacgcata aaagaattaa cagatcttct taaccgttac cgttatgact attatactaa
    12781 agatgcccca tcagtttctg acagtgacta tgataagctt tatcgtgaac tggtgacttt
    12841 agagcagtca tatcccgagt atgtcttgca agatagccca acgcagcagg ttggagggac
    12901 tattttaaag gggtttgaaa aatatcgaca tcagtatccg ctttttagtt tgcaggatgc
    12961 tttttcacgt gaggagctgg atgcttttga taagcgcgtc aaagccgaat ttccaaatgc
    13021 cacctatcta gcagagttaa aaattgatgg tttatctatt tccttgagtt atgagaatgg
    13081 cttcttacag gttggagcaa cacggggtga tggcaatatc ggtgaaaata tcacagaaaa
    13141 tatcaaaaaa ataaaggata ttcctcacca attgagtgag cctttaacca tcaccgttcg
    13201 aggagaggct tacatgtctc gtcagtcctt taaagcgatc aatgaagcac gccaagaaaa
    13261 tggggagact gaatttgcta atcctagaaa tgctgccgct gggacactaa ggcagctaga
    13321 tacggcagtg gttgctaagc gtgaactagc cacttttttg tatcaagaag ctagtccgac
    13381 agctagaaat caacaaaatg aggtcttagc agaattagca gatctaggtt tttcagtaaa
    13441 tccgtattat caactcacat cttctatgga tgagatttgg gattttatca aaactattga
    13501 ggcaaaaaga gatcaattag cttatgacat agatggcgtt gttattaaag ttaatagcct
    13561 agccatgcaa gaagagctag ggtttacggt taaagctcca cgctgggcca ttgcttacaa
    13621 atttccagca gaagaaaaag aggcagaaat cctttcggtt gactggacag tgggacgaac
    13681 aggcgtggtc acacctacag cgaatctaac tcctgtgcaa ctagcgggga caaccgtgag
    13741 tcgtgcaacc ctacataatg tggattatat tgccgaaaaa gatatccgta ttggcgatac
    13801 agtgattgta tataaggcag gggacattat tcctgctgtc ttaaatgtcg ttatgagtaa
    13861 gcggaaccag caagaagtca tgcttattcc taaactatgc ccatcttgtg gtagtgaatt
    13921 agttcatttt gaagatgagg ttgctttgcg ttgtattaat ccgttgtgtc ctagtttgat
    13981 tcagcgaagt ttggagcatt ttgcaagtcg tgatgccatg aacattactg ggctgggacc
    14041 agctattgtg gaaaaactat ttttagcggg gtttgttcat gatgttgctg atatttacca
    14101 gttgaccaaa gaaaatttca tgcaattaga tggcatcaaa gaaaagtcag ctgataaatt
    14161 gctcgctgcg attgaggctt ctaaatcaaa ttcagctgaa aaactgttat ttggtctagg
    14221 aattcgtcat attggctcta aagttagtcg cttaatttta gaggtatacg gagatatttc
    14281 tgctctttta accgctaaag aagaggagat cgcaaggata gatggccttg gctcaacgat
    14341 tgctcaatca cttactcaat actttgaaca aaaaacggct gcgattttag tagacgagtt
    14401 aaagacagca ggtgtcaaca tgcattactc aggtcaaaaa gttaatagtg acgcagcgct
    14461 ttttggtcta actgttgttt taactggcaa attaaatcag ctgaaccgaa atgaggccaa
    14521 agataaatta gaggcattag gtgccaaagt tactggaagt gtatctaaga aaacagattt
    14581 agttattgca ggtagtgacg ctggttctaa gctagaaaaa gcaaagagct taggaattcg
    14641 gattgaagat gaagattggc tacgaaagct ttaagtgagg taatgttatg aaaaaacaat
    14701 tgagagcacg tctcatttat aatccaacgt caggacaaga acttatgaga aaaagtgttc
    14761 ctgaagtttt agacatatta gaagggttcg ggtatgaaac atctgccttt caaacaacag
    14821 cacaaaagaa ctctgctcta aatgaagcaa gaagagcggc aaaggcaggt tttgatttat
    14881 tgattgctgc tggaggtgat ggcactatta acgaagtggt caatggaatt gctcccttga
    14941 aaaagcgccc taaaatggcc attattccaa caggtaccac taatgatttt gctagagcgt
    15001 taaaagtacc aagaggaaat ccgagccagg cagcaaaact tatcggaaaa aatcagacta
    15061 tccagatgga tattggtaga gctaagaaag atacttattt catcaatatt gctgcagcag
    15121 gaagtttgac ggaattaact tacagtgtac caagtcagtt aaaaacgatg tttggatact
    15181 tggcttatct tgctaaagga gtagaactgc tcccaagagt tagcaatgtg cctgttaaaa
    15241 ttacgcatga caaaggcgtc tttgaaggtc aagtttctat gatttttgca gctattacca
    15301 attctgttgg gggatttgaa atgattgctc ctgatgcaaa actagatgac ggtatgttta
    15361 cgctcatttt aataaagaca gcaaatcttt ttgaaattgt acatcttttg cgtttgattt
    15421 tggatggtgg taagcatatt acagatcgcc gtgtcgaata cattaagacc agtaaaatag
    15481 tcattgaacc ccaatgtggt aagcgaatga tgattaattt agatggcgag tacggaggag
    15541 atgctccaat tacattagag aacctaaaaa atcatatcac tttttttgca gatactgatt
    15601 tgatttctga tgaagcttta gtcttagatc aggacgaatt ggaaattgag gaaatcgtaa
    15661 agaaatttgc tcatgaagta gaagacttag aacaagaatt agaagaataa tacaatagtt
    15721 attagctact gacagtttag tcaaatgcac tactattaga atctaagatt tttaaatcaa
    15781 ttgaggtagt gttttttgat gcgctcaaag agtcaattta ttcatatgag gggcccattt
    15841 gaggtctctc ttttttgatc atatgcaaga aaatgccatg ttttaggggt gttattatac
    15901 cctttttgga atcttgttgc ctttttgtta taataagagt gtaattctat ttttaaaaag
    15961 atcttaacaa agacagactc gtttatcaaa aaggttagta ttagtctaag aaagcgtact
    16021 cagtctaaag aaatctgttg acattcttaa ttttaaggaa tagaatagca aagtaaaaaa
    16081 aatttaaagg agtattctac tatgaatcct attttcgcat tggctttggc ctgttttggt
    16141 gtttctcttg ccgaaggttt cttgatggca aatcttttta aagcagcatc tcgccaacca
    16201 gaaatcattg gtcaattacg ttcattaatg atcttgggtg ttgcctttat cgaaggtact
    16261 ttctttgtta cccttgttat ggccttcatt cttaaataac agcctatttg ataatagaaa
    16321 aggagggtgt tagatggaag aagctaaaat acccatgcta aagcttggtc caataacctt
    16381 taatttgacc ctacttgctg tttgtattgt cacaattgct gttatctttg cctttgtttt
    16441 ttgggcaagt cgccaaatga aattgaaacc agaagggaag caaactgctt tagaatattt
    16501 aatcagtttt gtggatggta ttggagaaga gcacttagat cataatctac aaaaatctta
    16561 ttcgctgtta ctctttacca tttttctctt tgtagctgtc gctaataatt tagggttatt
    16621 tactaagtta gagacagtta atggctataa cctatggacc tcgccaacag ccaatctggc
    16681 ttttgacctt gctctatctc tttttattac cttaatggta cacattgaag gggttagacg
    16741 tcgtggcttg gttgcccatt tgaaacgttt ggctacaccg tggccaatga ctccgatgaa
    16801 tttattagaa gagttcacaa atttcttatc acttgccatt aggttattcg gtaatatctt
    16861 tgccggggaa gttgttacag gtttgattgt tcaactggcc aattatcgag tttattggtg
    16921 gccgatcgct ttcctagtca atatggcgtg gacagccttt tcagtcttta tttcctgcat
    16981 acaggctttt gttttcacaa aactaacagc gacctatcta ggaaagaaag ttaatgaatc
    17041 agaagaataa ggaaggagaa caaacgaatg tcaatcacat ttggtgaact cgttgggaat
    17101 tttattctag taacaggttc tgtaatcgtc ttgttactct tgatcaaaaa attcgcttgg
    17161 ggtgcaattg aatcgatttt acagacacgc tcacaacaaa tctctcgaga tattgatcag
    17221 gctgagcaat cacgtctaag tgctcaacag ttagaggcaa aaagtcaagc taacctagat
    17281 gctagtcgtt cacaagcaag taaaatcatt agtgatgcca aagaaattgg tcaattacaa
    17341 ggtgataaat tggtggcaga agctactgat gaagcaaaac gcttgaaaga aaaagcgttg
    17401 acagatattg aacaaagcaa atcagacgct atttcagcag tcaaaacaga aatgtctgat
    17461 ttaacggttc ttttagcgga aaaaattatg ggagccaatc ttgataagac ggcgcaaagc
    17521 cagcttattg acagttatct tgatgactta ggagaagctt aatgaccaaa aaagaacagg
    17581 ctcttatcga gcaatatgca aaaagccttg ttgaggtggc aagtgaacat cattctcttg
    17641 atgccttaca agctgatgtg cttgcgattt tagagacgtt tgtaacaact aatcttgatc
    17701 aaagtctatc aagtctagct gttccgcatg ctgaaaaaat aaaattgcta accttattaa
    17761 agggaaataa ttctgtttat atgaataatt tcttgaatct cattttacaa aacgaacgtg
    17821 aagcttattt gtatcaaatg cttcaggctg ttttaaatga aattgcaata gtttcaaatc
    17881 agtatgatgt gacagttacc tcatcactac ctttgactga ggagcaaaaa agccgtgttc
    17941 gagcagttgt tgcaaagaaa tttgctgtaa cagcgggtcg tttaattgaa aaagtagatc
    18001 cttcgcttat tggaggattt attatcagcg tcaataataa agtaattgat accagtattc
    18061 gtcgtcaatt gcaagcattt aaaatgaatt taaaatagaa agtggtgtga cttttggcaa
    18121 ttaatgcaca agaaattagc gctttaatta aaaagcaaat tgagaacttc cagccaaatt
    18181 tcgatgtcac agaaactggg atcgttacct atattggtga cggtattgca cgtgcccgtg
    18241 gattagataa tgccatgagt ggtgaacttc ttgaatttga aaatggtgcc tatgggatgg
    18301 ctcaaaacct tgagtcaaat gatgtgggta ttattattct tggtgatttt tcagcaatcc
    18361 gtgaaggaga tgtggttaag cgcaccggta aaatcatgga agtaccagtt ggtgaagctc
    18421 tcattggacg tgtggtaaac ccacttggac aaccagttga tggcttggga gatattgaaa
    18481 caactggctt tagaccagta gaaacgccag ctcctggtgt tatgcagcgt aaatccgttt
    18541 ctgaaccctt acaaactgga ttaaaagcaa ttgatgcttt ggttccaatt ggtcgtgggc
    18601 aacgtgagtt gattatcgga gaccgtcaaa caggaaaaac atccgtagct attgatgcga
    18661 ttttgaacca aaaaggtcaa gacatgattt gtatctatgt ggctattggt caaaaagaat
    18721 caaccgttcg tacacaagtt gaaacccttc gccgttacgg tgccttggat tatactattg
    18781 ttgtgacagc gtcagcctca caaccatcac cattgctctt tatcgctcct tatgcgggtg
    18841 ttgccatggc tgaagaattt atgtaccaag gcaagcatgt cttgattgtc tatgatgact
    18901 tatcaaaaca agcggtggct tatcgtgaat tgtctctctt gcttcgtcgt ccaccaggtc
    18961 gtgaggctta cccaggggat gttttctact tgcatagccg tcttttggaa cgctctgcaa
    19021 aagtttctga tgatttaggt ggaggttcta tcacagctct accatttatc gaaacacagg
    19081 ccggagatat ctctgcatat attgcgacta acgttatttc gattacagac ggacaaatct
    19141 tcttgcaaga gaacctcttt aattcaggaa ttcgtccagc gattgatgca ggatcatctg
    19201 tctcacgtgt aggtggttca gctcaaatta aagcgatgaa aaaggttgct ggtactcttc
    19261 gtttggattt ggcttcctat cgtgaattag aagcctttac ccaatttggt tcggaccttg
    19321 atgctgcaac acaagcaaaa cttaatcgtg gtcgccgtac ggttgaaatc ttgaagcaac
    19381 cattgcataa accgttacct gttgaaaagc aagtcgttat tctttatgcc cttactcatg
    19441 gtttcttaga tgatgttcct gtagatgata ttttggcttt tgaagaagct ctttacgact
    19501 actttgacgt tcattacaat gatttatttg aaactatccg taccacaaaa gaccttcctg
    19561 aagaagctgc ccttgatgcc gctattaaag cgtttaaaga acactcaaac tttaagtcat
    19621 agagggagga tactagtatg gcaggctctc taagtgaaat taaagctaaa attatttcaa
    19681 ctgagaaaac aagtaaaatc actagtgcca tgcgtatggt ttcttctgca aaacttgtta
    19741 aatctgagca agcagctcgt gattttcaaa tttacgcctc aaaaattcgc cagattacta
    19801 cagatttatt gaaatcagaa ttaaccatag gatctgataa tccgatgttg gtgtctcgtc
    19861 ctgtcaaaaa aacaggctat attgtcatca cgtctgataa aggacttgtt ggaggttaca
    19921 attcaaaaat cttgaaatct gttatggata tgattacaga gtaccatgct gatggtgact
    19981 atgaaatcat ttctattggt agtgttggtt cagacttctt caaagcaaga gggatgaatg
    20041 tggcttttga acttcgtgga cttgcggatc aaccaagttt tgaacaagtg agacaaatca
    20101 tttcacaatc cgttgacatg tttgtcaatg agatttttga tgagttatat gtctgttaca
    20161 atcaccatgt gaatagctta accagccaag tccgtgttca acagatgttg ccaatttctg
    20221 acttggttgc tgatgaagca gcagaagaag gtgttactgg gtttgaattg gaacctaatc
    20281 gtcatgatat tttagatcaa ttgttacctc aattcacaga aagtttaatt tatggggcta
    20341 tcattgatgc taaaacggct gaacatgcag caggtatgac tgctatgcag acagcgacag
    20401 ataacgctaa gaatgtcatt aacgatttga ccatccaata taaccgagct cgtcaagcag
    20461 caattacaca agaaattact gaaattgttg caggagcaaa tgcactagaa taaaaaatga
    20521 cgctgactag cgattaggtt attgtcagca ttcaatttta tcaatcgaga taaaatgact
    20581 tatgggactt ttctattgaa agactgctct tttcagtatg aaagaagtta gcaagactaa
    20641 ctaagccctg actatcgaat ataaggagaa atgaatgagc tcaggcaaaa ttgctcaggt
    20701 tgttggtcca gtagtagacg ttatgtttgc tagtggggac aaacttccag agattaataa
    20761 tgcattgatt gtttataaag atagtgataa aaagcaaaaa atcgtccttg aagttgctct
    20821 ggaacttggt gacggtatgg tgcgaacaat cgctatggaa tcaactgatg ggcttacacg
    20881 tgggttagaa gttcttgaca ctggtcgtgc gattagcgta ccagtaggta aagaaacttt
    20941 gggacgcgtc tttaatgtac ttggagaaac cattgacttg gaagaaccat ttgcagaaga
    21001 tgttgaccgt cagccgatcc ataaaaaagc accatcgttt gatgaattat caacatcatc
    21061 agaaattctt gaaactggta tcaaggtaat tgaccttctt gccccgtacc ttaaaggtgg
    21121 taaagttgga ctttttggtg gtgccggagt tggtaagact gtccttatcc aagaattaat
    21181 ccacaatatc gcccaagaac acggaggtat ttcagtattt accggtgttg gtgagcgaac
    21241 acgtgaagga aatgaccttt actgggaaat gaaagaatca ggcgttattg aaaaaaccgc
    21301 catggttttt ggtcagatga atgagccgcc tggggcacgt atgcgtgtag cccttactgg
    21361 tttaaccatt gctgagtatt tccgtgatgt agaaggccaa gatgttttgc tctttattga
    21421 taatatcttc cgtttcactc aggcaggttc agaagtatca gccctcttag gccgtatgcc
    21481 ttctgctgtt ggttaccaac caacccttgc tactgaaatg gggcaattgc aagaacggat
    21541 tacatcaact caaaaaggat ctgttacatc tatccaagcg atttatgtac cagcagatga
    21601 ttatactgac cctgcgccag caacagcatt tgctcacttg gattcaacaa ctaaccttga
    21661 acgtaaattg actcaaatgg gtatttatcc agccgttgac ccattggctt caagttcacg
    21721 ggccttatca cctgaaattg ttggtgaaga gcactatgct gtggcaacag aagttcaacg
    21781 tgtgcttcaa cgctaccgtg aattacaaga catcatcgct attttgggga tggatgaatt
    21841 gtcagatgaa gaaaaaacat tggttggacg tgcgcgtcgt atccagttct tcctctcaca
    21901 aaattttaac gttgctgaac aatttaccgg tcttcctggt tcttacgtgc cagttgctga
    21961 gacagttcgc ggctttaaag aaattcttga aggaaaatac gatcaccttc cagaagatgc
    22021 ctttcgttca gttggtccaa tcgaggatgt gattaaaaaa gccgaaaaaa tgggctttta
    22081 gtgaggtgat gccatgacac aaatgactgt tcaagtggta acgccagatg gaatcaaata
    22141 tgatcaccat gctaaatgta tttctgtaac aacaccagat ggggaaatgg ggattttacc
    22201 taatcacatc aatttaattg cacccttgca agttcatgaa atgaaaattc gccgaggtgg
    22261 tgaagatgaa aaagtggact ggattgctat caatggtggt attattgaaa tcaaggataa
    22321 tgtggttacc gttgtcgctg attctgcaga acgtgaccgt gatatcgatg tcagccgagc
    22381 agagcgagca aaactaagag cagaacgtga aattgcacag gctgaaacga cccacaacat
    22441 tgatgaagtt cgtcgtgcca aggttgcctt gcgccgcgct ttgaatcgta tcaacgttag
    22501 taaaaaataa gtggtgttga taaaagacaa ggataagtaa gaaaggtttt taacctatta
    22561 ttttgtttgc tgagtctttt taacatcagt ttctgagagt ttgaccatac taccttctct
    22621 aaacacgtta ttattgttga gaggagttat atgggggaac tcttttttgt atccacgttg
    22681 ttagtagaaa ctcagtaatg acgcttaatg tattttcagg ctatcaactt agtcagagag
    22741 gatgtcaatt taattgataa gttggacgga tcaagatggt atgttataat aatcctatgg
    22801 aattattaat gaagatcatt gccttttcgt gtcatttatt ttttattggc cttagttacc
    22861 aattattgat tagtgtgatt gactggacaa aatttagtca ttatcatcca gaaaatcttg
    22921 gccggctacg actttttgtc tttttattag ctactgccct agggtatttg gtgagccatt
    22981 gtatgctgga attgcttcaa attagccaga cgctcttttt tgagttccgc taatactgga
    23041 ataaaagaag tatggtataa ttttactacg actaagagat agttaaggga gaaaattgtg
    23101 gataaaataa tcattgaagg tggtcaaaca cgcttagaag gagaagttgt tattgagggt
    23161 gctaaaaacg cagttcttcc tttactggca gcatctattt taccttcaaa aggaaaaaca
    23221 attcttagaa acgttcctat tctatctgat gtttttacta tgaataacgt ggtacgtggt
    23281 ttagatattc gtgttgattt taatgaagca gctaacgaaa tcacagttga tgcctcagga
    23341 cacattttag atgaagcccc ttacgagtac gtgagccaaa tgcgtgcctc tattgttgtt
    23401 ttaggaccca ttttggcacg taatggccac gcaaaagtat caatgcctgg aggctgtacg
    23461 attggcagta ggcctattaa tttgcactta aaaggattag aggctatggg ggcaacgatt
    23521 actcaaaaag gtggagatat tactgctcaa gcagatcgac tgcaaggtgc catgatttat
    23581 atggacttcc catctgtagg agctacccaa aacttgatga tggctgccac tttagctgat
    23641 ggtgtgacca ctattgaaaa tgctgctcgt gagcctgaaa ttgttgattt agctcaattt
    23701 ttgaataaaa tgggagctag aattcgtggt gctggaacag agacgttaac gataacaggc
    23761 gtcacgcatt tacgaggcgt agaacatgat gttgttcaag atcgtataga agccggtaca
    23821 tttatggttg cagcggcaat gacttcaggt aatgttttga ttagagatgc tgtttgggaa
    23881 cataatcgtc cattgatttc aaaattaatg gaaatgggtg tttctgtaac agaagaagaa
    23941 tacggtattc gcgttcaagc aaacacgcca aagctcaaac ctgtaacggt taaaaccttg
    24001 cctcatccag gttttccaac agacatgcaa gctcaattta cagctttaat ggcagtggtc
    24061 aatggagagt ctactatggt tgaaactgtc tttgaaaatc gatttcaaca tctggaagaa
    24121 atgcgacgca tgggcttaca atctgaaatc cttagggaaa ctgccatgat tcatggtgga
    24181 agacaactgc aaggtgctcc tgtgatgtca acagatttac gtgccagtgc tgcccttatc
    24241 ttgacaggga ttgttgctca aggagttaca atagttaata atttggttca tttagatcgt
    24301 ggctactatc aatttcatga aaaattagca aagcttggag ctactattag tcgtagcagt
    24361 gaggtctagc ttatgacaaa tggtttaaaa tatgtcttag aacaaatgtt attacttttc
    24421 ataattgctg ccttggcgtg tcttttttta gccattggac ttatgattgg ttatagtttt
    24481 atgggagatg gccatagtcc atggcatatt ttatctatgg ataagtgggc agagttagtg
    24541 aataaattta ctggaaagta ggccttgcct actttttata gcatctgaga ggaagtatgt
    24601 taacaaggaa aaaacgaaaa agggtgcaag agcaaaaggt tcctaaaaga gccaacgctt
    24661 tacttgctaa gtcaacccgg caatatcaag accgttcata acttaagaaa ggttaccgtt
    24721 catttaagcc agcaggttgg caccgattgc accacttaaa aggaagttat gatcacgcgg
    24781 ttgatagagg gcatttgcta ggttatgctt tagttggtgg actgaaaggt tttgatgctt
    24841 ctactggtaa tcctgataat attgccactc aactaagttg ggctaatcaa gcaaataaac
    24901 cctactcgac aggtcagaat tactatgaag gtttagtacg tcgtgctttg gataaggggc
    24961 atcgcgtccg ctaccgtgtc accctacttt atgatggtga taatctacta gccagcggta
    25021 gccatttgga ggctaaatcc tctgatgaca gcttggcttt taacgtgttt gttccaaatg
    25081 tccaagcagg attgaccgtt gactatcgga cgggacaaat agctgtcaac aattgccttt
    25141 tgcttatctc ttcagttgct gtgattttat catacagtga taaaagat
//
LOCUS       DAUCWO010000021        24822 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUCWO010000021 DAUCWO010000000
VERSION     DAUCWO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24822
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.021"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(176..2386)
                     /locus_tag="VGY20_000808"
     CDS             complement(176..2386)
                     /locus_tag="VGY20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ4273676.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLFGGALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
     gene            2494..3657
                     /locus_tag="VGY20_000809"
     CDS             2494..3657
                     /locus_tag="VGY20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ4273677.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQ
                     EGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDT
                     ETAHLYGELAQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLN
                     LFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYF
                     KYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAA
                     NMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            3654..4340
                     /locus_tag="VGY20_000810"
     CDS             3654..4340
                     /locus_tag="VGY20_000810"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ4273678.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            4434..5600
                     /gene="aroC"
                     /locus_tag="VGY20_000811"
     CDS             4434..5600
                     /gene="aroC"
                     /locus_tag="VGY20_000811"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ4273679.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            5661..6002
                     /locus_tag="VGY20_000812"
     CDS             5661..6002
                     /locus_tag="VGY20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ4273680.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            6224..7576
                     /gene="gorA"
                     /locus_tag="VGY20_000813"
     CDS             6224..7576
                     /gene="gorA"
                     /locus_tag="VGY20_000813"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ4273681.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHEAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSEHLFNNKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKKYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(7656..8933)
                     /locus_tag="VGY20_000814"
     CDS             complement(7656..8933)
                     /locus_tag="VGY20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ4273682.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFPLIT"
     gene            complement(8963..9403)
                     /locus_tag="VGY20_000815"
     CDS             complement(8963..9403)
                     /locus_tag="VGY20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273683.1"
                     /translation="MSSHYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNP"
     gene            9638..10780
                     /locus_tag="VGY20_000816"
     CDS             9638..10780
                     /locus_tag="VGY20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4273684.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKNAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKHEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            10792..12006
                     /gene="thiI"
                     /locus_tag="VGY20_000817"
     CDS             10792..12006
                     /gene="thiI"
                     /locus_tag="VGY20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ4273685.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            12044..13336
                     /locus_tag="VGY20_000818"
     CDS             12044..13336
                     /locus_tag="VGY20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ4273686.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNELAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    13441..13515
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            13550..13864
                     /gene="rplU"
                     /locus_tag="VGY20_000819"
     CDS             13550..13864
                     /gene="rplU"
                     /locus_tag="VGY20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ4273687.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            13876..14202
                     /locus_tag="VGY20_000820"
     CDS             13876..14202
                     /locus_tag="VGY20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ4273688.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            14230..14523
                     /gene="rpmA"
                     /locus_tag="VGY20_000821"
     CDS             14230..14523
                     /gene="rpmA"
                     /locus_tag="VGY20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ4273689.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            14871..15785
                     /locus_tag="VGY20_000822"
     CDS             14871..15785
                     /locus_tag="VGY20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4273690.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            15782..16240
                     /locus_tag="VGY20_000823"
     CDS             15782..16240
                     /locus_tag="VGY20_000823"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ4273691.1"
                     /translation="MKKRLFVPSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMIHLDFMDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            16230..17120
                     /locus_tag="VGY20_000824"
     CDS             16230..17120
                     /locus_tag="VGY20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4273692.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            17516..18037
                     /gene="pyrR"
                     /locus_tag="VGY20_000825"
     CDS             17516..18037
                     /gene="pyrR"
                     /locus_tag="VGY20_000825"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ4273693.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDTKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            18053..19312
                     /locus_tag="VGY20_000826"
     CDS             18053..19312
                     /locus_tag="VGY20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ4273694.1"
                     /translation="MKDVSYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPILLGLLVGYVVALLVGVFTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKILSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            19373..20308
                     /locus_tag="VGY20_000827"
     CDS             19373..20308
                     /locus_tag="VGY20_000827"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ4273695.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            20352..21434
                     /gene="carA"
                     /locus_tag="VGY20_000828"
     CDS             20352..21434
                     /gene="carA"
                     /locus_tag="VGY20_000828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ4273696.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            21680..>24822
                     /gene="carB"
                     /locus_tag="VGY20_000829"
     CDS             21680..>24822
                     /gene="carB"
                     /locus_tag="VGY20_000829"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ4273697.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTV"
BASE COUNT         7687 a         4236 c         5292 g         7607 t
ORIGIN      
        1 tttcccatgt tttattacca gtaatgtggg atatttagat ggtagcaaga agttttatag
       61 ttgatttgtt ttctttaggt ctaattagca tattttgatt actgataaac ttgaatatcg
      121 ccaataaaaa agaagcaaaa ttattatttt gcttctagtc agatcattaa attgattatt
      181 ctctattttt ggtgttatcc tcttttagtt cagaagttgc cgcgtcagct tctacgggat
      241 ttagttctaa gtaagatggt gctttaaaaa gtttctgagt tgatttatga ccatttttac
      301 tgtataaact tgttgactct gatccgagtt ttgctgaaat atctttcagt tgttttaact
      361 gcttagtgta gataaactgg ttaggatcaa tagcttttag accattttga gtatcaaagc
      421 gcaggagatc acccgtttga atagcgtcgc tagctgctag ttgttgagca acagcagagc
      481 gaatttcctt agttttggct atagtcattt catcgggatt agtgatttct aaacctgttt
      541 gcgtgttgta cagacgtcca ctatagttag tgtattcagg agtcatataa gtcccagatg
      601 ttcgttgtgc cacaatctgg ctattttgag gtgataataa atcttgtcct aattgaacaa
      661 actgactagt gtcaatacca agtatgtgga gtaaagtagg aagagcatcg atttcaccgc
      721 caaaggtttc tttgatacta ccattcgtat aacctggaat atggatcata tacgggactc
      781 gttgtagcat agcattatca tattcagacc aagtttcaga atctttacca agaagtggag
      841 caagactaga attacgagaa tttgaaatac cataatggtc accatataaa acaaaaatag
      901 aattgtcgta caaaccagtg gctttcaagt aatcaaaaaa agatttaagg gcagcgtcta
      961 aataattggc agtagcaaag taaccattga tcgtttcatc gtctgttttg gctaaaggaa
     1021 aaccttcttc actactttcg ccttttagac tagtataagg atagtgatta ctcacagtga
     1081 taaacttggt ataaaaaggt tgttgcatct gttcaagata ttttatggaa tccttaaaca
     1141 tgtacttatc atttaaccca tattgaaaag agttcttact attttgttta gagaagtagc
     1201 tagaatcaaa aaaataatta tagccccatt gtttataagc attattgcga ttccagaaag
     1261 ttccaacgtt accatgaaag acagcgctgg tatagccacc tttttgggtt aaaatacttg
     1321 gagtagcaaa ttgtgtattt tcaccaccgt agttcaccat aaaagaacca ctatttaaac
     1381 caaataagga attctccatc atggtttcag catcagaagt tttacctgct ttaacctgat
     1441 gaaaaaagtt agggaatgct aaggtagcat ttgagtgata aagcgagttg ataaaaggcg
     1501 ttacctcata ttctttatct ccttctttta gtttataatc tattaagaat tgttgaaagc
     1561 tttctaaatg aagaacaata atatttttgc cttttccaat tccaaaatac tgaggatcag
     1621 gtgctgcata atgtcctttg acataagttt taacatcaat taattcttct gcagtagctc
     1681 catttcgttc tttttgagct tgataggttt gattgccgct atagagggta aacgctggta
     1741 gtcctaaagc tctcacaata taagtatttg aaaagccacg tgttaatagt tccggacgat
     1801 caatttctgc taaaaaaaga ttaatagaaa gcaatagact agataaagcg gtgatggcaa
     1861 atgctgcgcg cttgttaaaa ggacgttggt catttttgat ttttttagtg atagataaac
     1921 taattagaat aataaaatca agaatataga taatatccca gatgcgtaaa aggtttaaag
     1981 cagagtcccc taatccagcg gagactttac tactagctag catggcactc actgtaataa
     2041 agtctgaaaa ttctcggtaa tatatagaat ttgatatcaa gagaatgtta aggataatat
     2101 aaaccaccca agaacagata taaaaggctc gggtattctt aacgtaaaga gcacccccaa
     2161 aaagtaaaaa agctagtggg attggattga taatagttag aaagacttgg tatatatttc
     2221 ccaaatctag tgaaaagtct gtatgatatg cccacagggt ctttatccag taacaaaaga
     2281 gtagagtaat tataaaaccc aatcttgtgt tgataaaacc tgtgataagt gttttaaatt
     2341 tcttcacagt agcaacttcc ttatcgtttt ttcctaaaga tgtcatctcc aaataaaatc
     2401 tcagattaaa ttataccaaa aatgtgaagc taatgcttgt tgtaagttca aatttagtag
     2461 gattttttat cagattttgt tataataaaa actatgaata aactctatat tgattctttt
     2521 gtcgaaaaga agctgacagc aggggtacaa ttattagatg aaaaagattt ttctaatata
     2581 aaggaaaaaa atcagttggt tcaactggtg accaagtcca atcgtcccat tggaacagca
     2641 tatatctcta agcaaaataa aggaattggt tggtatctag gaccagaaaa aatcgacttg
     2701 tctatctctt attttgttag tctattttca gttgctaagg ccaaaagaca agattttgct
     2761 caatcggacg agaccaatgc ttatcgcctt tttaatcaag aaggagacgg ttttggtgga
     2821 gtaacaattg atctttataa agattttgca gtcttttcat ggtacaatgc ctttgtctat
     2881 gacaaaaaag aaatgattat ggaggctttt caacaggttt ttcctgaagt caaaggagct
     2941 tatgaaaagt gtcgctttaa aggtccagat actgaaactg ctcatctgta tggtgagctg
     3001 gcacaagaaa ccttttcgat tttagaaaat ggcatagcct atcaagtctt cttgaatgag
     3061 ggcttaatga caggtatttt tctagaccag catgatgtta gaagagcact agtagatggt
     3121 ttagctatgg gcaagtccct tcttaaccta ttttcctata cggctgcctt ttctgtagca
     3181 gctgctatgg gtggtgctat cgagaccact tctgtggact tggctaaacg atcgagagaa
     3241 ttatcactag ctcattttga gcataaccag ttaaacttag cctcacatca ttttgtggtg
     3301 atggatgttt ttgaatactt caaatatgcc aaacggaaaa aattaatctt tgatgtgatt
     3361 gtgattgacc caccaagctt tgctagaaat aaaaaacaaa ctttctctgt gagcagggac
     3421 taccacaaat taatcacaga ggccttggac atattgagtc ctaagggaac tattatagct
     3481 tcaacaaatg ctgctaatat gactgttagc cagttcaaaa agcaaattat caaaggattt
     3541 ggaagtcgtc gtcctgagtc tatgactctc cagcagcttc cgagtgactt tactatcaat
     3601 aaagcagatg aaagaagtaa ctatttaaag gtatttacta taaaggtacg agaatgagaa
     3661 ttgtagcacc agtcatgcca agacattttg acgaggctca agccattgat atttctaaat
     3721 atgaagatgt taatttaatt gaatggcgag cagactttct tcccaaggat gagattgtcg
     3781 cagtagcacc agctattttt gaaaaatttg ccggaaaaga aattatcttt acccttcgga
     3841 ccgttcaaga aggggggaat attacccttt caagtcagga gtatgttgat attatcaaag
     3901 aaatcaatgc tatttataat ccagactata ttgactttga gtattttacg cacaagtcag
     3961 tatttcaaga aatgcttgat tttccaaatc ttattttgtc ttatcacaac tttgaagaga
     4021 ctcctgaaaa tttaatggaa gctttttcag aaatgaccaa gttggcaccg cgtgtggtta
     4081 aaattgctgt gatgccccag agtgaacaag atgttctaga tttgatgaac tataccagag
     4141 gctttaagac gctcaatcct gagcaagaat ttgcaactat atctatgggg aaattaggaa
     4201 gattgtctcg ttttgcggga gatgtcattg gctcttcctg gacatacgtt tcattagatc
     4261 atgttagcgg tccaggtcaa gtgactctca atgatatgaa acgtattatt gaggtcttag
     4321 aaatggatat ctctaactag tgaatggtct aaaaatatta tttctacaat agttaagaaa
     4381 tgatgttttt agattaatgt ttttaaaata agtggaacca aaaaggagaa aaattgcgtt
     4441 atttaacagc aggagaatct catgggccat ccttaacagc tattattgaa ggtataccag
     4501 caggattaac tcttcatcca gcggatattg atcacgaatt acagcgacgt caaggaggtt
     4561 atggccgtgg tgccagaatg tctattgaga ctgatcgggt tcaaatatcc tcaggagttc
     4621 gtcatggcaa aacaacaggg gcccccatta ccttaacggt aattaataag gaccaccaaa
     4681 aatggctaga tgtcatggct gtaggagaca ttgaagagac tctcaagtta aagcgtcgtg
     4741 ttaagcatcc tagaccagga catgcggatt tggtaggagg tattaaatat cattttaacg
     4801 atttaagaga tgctttagag cgttcctcgg ctagagaaac aacgatgaga gtggctgtag
     4861 gcgctgttgc taaacgtatc ttagctgaac ttggaattga tatgttacat catatcttaa
     4921 tatttggtgg tataacgatc acaatacctt ctaagctctc ttttagagag ttgcaagaaa
     4981 gggctctcca ttcagaatta tctattgtta atccaaagca agaagaagaa attaaaacgt
     5041 atattgataa gataaaaaaa gaaggggata ctattggtgg tattatcgaa acgatagttc
     5101 aaggggtgcc agcaggtctt ggatcttatg tccaatggga taaaaaatta gatgccaagc
     5161 tggctcaagc agttctttcc attaatgctt ttaaaggggt ggaatttgga gttgggtttg
     5221 atatgggatt ccaaaagggc tctcaggtaa tggatgaaat cacctggaca cctacccaag
     5281 gctatggtcg tcaaacaaac cacttaggtg gctttgaagg tggcatgacc acaggacaac
     5341 ctcttgttgt taaaggggtt atgaagccca ttcctacttt gtataaacca ctcatgtcgg
     5401 tagatattga tagtcatgaa ccttataaag caacggtgga acgctctgat ccgacggcct
     5461 taccagctgc aggggttatc atggaaaatg tggtggcgac ggtgcttgcc aaagagattt
     5521 tagagacttt ttcttctact accatgtctg aattgcaaaa agctttttcg gactatcgtg
     5581 cctacgtcaa gcaattttag atagcgagat actggctttt atggtatcat acaggtatta
     5641 ataaacaaaa ggagactgtc atgtcacaag aaatttatga ctatgctaat caacttgaaa
     5701 gagcagttcg tgctctgcca gaataccaaa aagtgttaga agtaaaagag gccattcaag
     5761 cagacgctag tgctagcgaa ctgtttgatg agtttgtcgc tatgcaagaa aaaatccaag
     5821 ggatgatgca atcaggccaa atgccaacgg ctgaagaaca aacaagcatt caagaactta
     5881 gccaaaagat tgaagctaat gaccagctaa aagcttattt tgaagcgcag caggccttat
     5941 ctgtttacat gagtgatatt gagcgtattg tatttgctcc gttgaaagat ttagtcaagt
     6001 aagccaatta agataggaaa aaaaacagat tgctggcagc tcgtcaggat ctgtttttta
     6061 gtgtttattt tctttgtatc agaaagttga gcctctttct tatttgtgat atagtcaaat
     6121 aaattgacca aaatagcagg ccagtagtgg ctttaatgaa ggcaagttgt ctgctaactg
     6181 gctatgctaa aacatcagta ttagaaagta aaaggttata aaaatggtta ttccttatga
     6241 ttatattgtt attggtggag ggagtgcagg tattgcttct gcgaacaggg cagccatgca
     6301 tgaggccaaa gtattgttag ctgaaggtaa agagattggg ggaacctgtg ttaacctagg
     6361 ctgcgttcct aaaaaagtca tgtggtatgg cgctcaagta gcagatattt tagggactta
     6421 cgctaaggat tatggatttg actttaaaga caaagcattt gattttaaac aattgaaagc
     6481 aaatcgtcaa gcttatattg atcggattca tgcttcttat gagcgaggct ttgagcagaa
     6541 tggtgttgac cgtatttatg attatgctgt tttcaaagat gctcatacgg tagaaattgc
     6601 agggcagctt tataccgcac cgcatatttt gattgccaca ggaggacatc ctgtttttcc
     6661 agatattgag ggggcacaat acggtatcag ctcggacggc ttttttgcct tagatgaggt
     6721 accaaaacgt acggctgtgg ttggggcagg ctatattgct gtggaacttg ccggtgtttt
     6781 gcatgctttg gggtcgaaga cggatttgtt tatccgtcac gatcgacctt taagaagttt
     6841 tgacaaagcc attgtggatg ttttagttga ggaaatggca gttggtggtc cttgcttaca
     6901 ccctcattca gaagtggcaa aggttgtcaa aaatactgat gagtccttga ccctttacct
     6961 caaagatggc caagaagttg aggttgacca gctcatttgg gctatcggtc gcaagcctaa
     7021 tctagaaggt tttggtcttg acaaaactgg agtgacgtta aatgataaag gttatattga
     7081 gacagatgcc tatgaaaaca cgtctgtcaa aggtatttat gccgtgggag atgttaatgg
     7141 gaaactggct ttaactccag ttgctgtggc agctggacgt cgcctatcag agcatctttt
     7201 caataacaaa acagacgaga aattggacta ccaaaacgtt gctactgtga tttttagtca
     7261 ccctgttatt ggctctgttg gcttgtcaga agaagcagca gtcaaaaagt atgggcaaga
     7321 agcagttaaa acatatcaat cacgttttac gtctatgttt actgctatca ctaaccatcg
     7381 ccaaccttgt ctgatgaagt tggtaacagt cggagatacc gaaaaaatag ttggtcttca
     7441 cggaatcggc tatggggttg acgaaatgat tcagggcttt gcagtagcta tcaaaatggg
     7501 agcaaccaag gcggattttg ataacaccgt tgctattcac ccaactggct cagaagaatt
     7561 tgtcaccatg cgctaaaaaa tgtagcctaa ggcaataaaa aatgatgtta acagttttcg
     7621 aggaaagtct tctaaactgc tagcatcgtt ttttgttagg tgattagggg gaatatgttt
     7681 ttaaagatat ttgcgttgct cagacaatat tgccttacct cggagataaa ataaagtgac
     7741 cctgtaacaa caaagagttc atcagatgtt acggaacctt gtaaccagtc tctgtaatct
     7801 tttacctgtg ggtatttatc aggataatcc tctaaaagat ttgcttcagg gaaatcaaaa
     7861 gtcgtgacag atacttcaaa agtatctagt tgagccagca agtcggctaa aggcttacgt
     7921 ttgagacctg caaagaggat atgaatagga cgtttgggaa attcttcttg cagtaaagac
     7981 tttaatttag caatggaatc tggattgtga gcgccgtcta atagaagatt aggttgaata
     8041 aattcggacc ggccaggcca agttgttgcc tcaattcctt tttgaatcgt ttttggagat
     8101 attttaggaa aggattttct aagtaattga gctgtcataa tggctaaaca ggcattcgct
     8161 ttttgatgtt gacctaatag tttgagacgg agtttggtta aattcaagtt atcgtgttga
     8221 aaggaaaacg tcttttctga aggattgagc aaaaaatctt ttcctaattg atataagcgt
     8281 gaaccggctt tttgggtaac ttgttgaaag acttgactag cctcctgctc aagttgaccg
     8341 ataataacgg gttctttagc tttgataact tctgcttttt ggtgagcaat ttctgctagg
     8401 ctataaccta aacgttcttg gtgatcaaaa ctaatggaag ggcatacgac ggccagagca
     8461 tgaaaaacat tggttgcgtc ggttttccca ccaatccctg cctcaataat ggcaatatca
     8521 acaggtttaa gattggcaaa gtagcaaaac attatgagag tcactaactc aaactcggta
     8581 ggtctatccc aatttgtttc aagggacatt ttagtgagaa tgggtctaat cacgctcaca
     8641 cattgcgcca attctaggtc tgaaatgggc tggccattaa ggcagatccg atcgtgaaaa
     8701 gagatgataa aaggagatgt aaaggttccg acctgataac cagaagttga caggatatgc
     8761 tgtaagtagg ctgtcacaga gcccttgccg ttagttccaa caacgtgaat agcagggaat
     8821 ttgtcttgtg gccggcctag tgcctcaagt aaccaagcca ttctggccaa atcggtcttg
     8881 cgaccattag ccttaaaggt atgaatccag gaaatgctat cataggtagt cattactatt
     8941 tcttgtcctt ctaacaatgc ttctaagggt tcgtaggaag accagtatta gcatcttcag
     9001 ctccagttgc attagggagt tgcgtccctt cagtggttga tggtgtctca ggggctgtag
     9061 gagttgtctc gttgtgagaa ggtgtggtag aagatgacgg aagagttgtc ttattatcat
     9121 ctgtggtttc tgaagtacct gtatggtttt gattaccctg agaagactct ttttcaataa
     9181 tcttgacaat ggtattactc gattctttgg gtgtatcatt agaggtctta tctggtacag
     9241 gctgcagtgc tcttgtaatg gtaatggttg ccacaatgat actcaaaata ctaccaataa
     9301 gtgcaatgga tttttggaaa gctgtaaaac ctgttttaat atgctcggta gggacacctc
     9361 ctgatttacg aggtttttgt tgacgggaat aatgtgatga catatttttc tcctttaaaa
     9421 tcatcgtaat gccaagatta aggaggagga aatagaagtc ctagctcctc tttaacactt
     9481 catctatcag tataatctag aaaaaaagtg aagtcaacgg tttaccttat gtctaggaca
     9541 aggaagtcct ttgggcttat tgtaatacga atattcttga tttcatttta gctattttgt
     9601 gatagaataa agtattgagt aaaaatgaag gtgacacatg atttattttg ataatgcagc
     9661 cacaaccata ccttatggag aggctcttaa aacttaccaa gaagtagcaa caaaaatata
     9721 tggcaatccc tcaagtctgc accaattagg gacaaatgcg tctcgcattt tagaagcttc
     9781 ccgtaaacag attgctggtt tgttgggtgt taagtcagaa gaaatctttt ttacgtctgg
     9841 tgggactgaa agtgctaatt gggctattaa gggaattgct tttgaaaaaa acgcttttgg
     9901 caaacatatt attatctcag ccattgaaca ccctgccgtg agtgaaagtg tcaaatggct
     9961 tctcacacaa ggattcgaag tttcttatgc tccagtgaca acacagggag ttgttgatgt
    10021 gaatgcttta gctgagctga ttagaccaga caccatctta atctcgatta tggctgttaa
    10081 taatgaaatg ggagctatcc aaccgattag agccatttca aacctattgg ctaaccaacc
    10141 aacgatcacc tttcacgttg atgctgttca agccattggt aaaataccac tctgtgatta
    10201 tatgactaat cgtgtggatt tggcttcttt ttcagggcac aaatttcata gtgttcgtgg
    10261 tgttggcttc ctttataaaa aagcaggcaa gcgactcaat cctctcttgt caggcggcgg
    10321 ccaagagcag gagttacgtt caaccactga aaatgttgca ggaattgcta gcatggccaa
    10381 agcattaaga atagtcactg aaaaacaagt gagcgtctta cctaaactaa cagcgatgcg
    10441 agatgtgatt tacaaagctt tgtccgctta cccagatgtg acagtatttt cagctcaaga
    10501 aggctttgcg cccaatattt tgacctttgg gattagaggg gttcgaggag aagttattgt
    10561 ccacgccttt gaaaaacatg agatttatat ttcgaccaca agtgcatgtt cgtccaaagc
    10621 aggtaagcca gcaggaagcc tagtggctat ggggattcct gtaaaaactg cccagacagc
    10681 cgttcgtatt agtctagacg atgacaacga tatgggacaa gtagagcagt ttttgaccat
    10741 attcaaacag atttatgata aaacacagaa agttaggtaa gattaggtaa gatggactat
    10801 tcagaaatta tggttcgcca tggtgaacta tcaactaaag ggaagaatcg catgcgattc
    10861 attaacaaac ttaaaaacaa tattcaagac gtactggctc ctttcccagc cattactgtc
    10921 cgttcagatc gtgataggac ccatgtgtct ctaaatggca cagattatca gcctattgta
    10981 gaagctttaa agctggtgtt tggtgttcaa gctctatctc ctgtttataa acttgaaaaa
    11041 agtgtccctc ttttggtaac agctgttcaa gatatcatga cttctcttta tcgtgatgga
    11101 ctgacgttta agattgccac taagcgtagt gatcacgcgt ttgaattgga tagtcgtgag
    11161 cttaacagcc ttcttggtgg agctgttttt gaggttttac ccaatatcca agcccagatg
    11221 aaacatcccg atgtgactct caaagtcgaa attcgtgatg aagcagctta catttcttat
    11281 gaagagatta agggagcagg cgggttacca gtaggaacgt cagggaaagg gatgctgatg
    11341 ttatcgggcg gtattgattc accagtagct gggtatttag cccttaaacg tggccttgat
    11401 attgaagttg ttcattttgc tagccctccg tacaccagtc caggtgcctt ggctaaagcc
    11461 caagacctta ctcgtcggtt aactcgtttt ggtggaaata tccaatttat cgaggttcct
    11521 tttacagaaa ttcaagaaga aattaaaaat aaggcaccag aggcttacct tatgacatta
    11581 acccgccgct ttatgatgcg catcacagat gccattcgtg agcaacgtaa gggtcttgtt
    11641 attgttaacg gtgagagcct aggtcaggtt gctagtcaaa cccttgaaag catgcaggcc
    11701 attaatgcgg taacctcaac cccaattatt agaccagtcg tgacaatgga taaacttgaa
    11761 attatcgaga tggctcaggc gattgatact tttgacattt ctattcaacc gttcgaagat
    11821 tgctgtacta tttttgcacc agaccgtcct aaaaccaacc ctaaactagg taatgctgaa
    11881 aaatatgaag aacgctttga tattgatggt cttgtccagc gtgctgtgtc aggaattgtt
    11941 gtcacagaaa tcacacctga aatcgtcaat gatgaggttg aaaatctcat tgatgcttta
    12001 ctttaaaaga aaagatcaag tgtaaatctt ttagtagtca gtaatgatac tattgaaaag
    12061 cctctataag ggcttttttg ctgcaatgaa ggaggaaaat tttgaggcaa gatgttataa
    12121 tagagaaaaa ggagggtgtg tcttgattaa aactatgcaa tacaaaaaaa cgatggcaac
    12181 agttgttgct cttatcgtcg cacttttgct atttggcttg atttatgatt tacttggggt
    12241 tcaaaaaaat gagctagcag ctcaaaaatc tgctcagcca aaggtcaaaa cagcgcgtgt
    12301 tgtagctaat ggcgacattt taattcatga tattctctac atgagtgcta gaaaagctga
    12361 cgatacctat gatttcactc cttattttga atatgttaag gactggatta gcggtgctga
    12421 cttagctatt ggagattatg aagggaccat tagcccagat tatcccttag caggctatcc
    12481 cttattcaat gctccagaag agattgcagg tgctctgaaa aatactggct atgatgtagt
    12541 cgatttagcc cataatcata tcttagattc ccaattagac ggtgccctta acactaaaaa
    12601 ggtttttcat caattaggta tagacagtat tggcatctat gacaaggatc gttcaaaaga
    12661 atccttctta attaaaaatg tcaatggtat caaaattgca attttaggtt attcttatgg
    12721 ttataacggc atggaggcca cacttagcca agaagactat gagaaacaca tgtctgattt
    12781 agatgaagct aagataaaaa aagaacttca gctagctgaa aaaaaggctg acgtgaccat
    12841 tgtcatgcca caaatgggaa cagaatatgc cttagaaccg acagcagagc aaaaagaact
    12901 ttaccacaaa atgattgatt ggggagctga cgtcgtccta ggaggccatc cgcatgttat
    12961 tgaaccttca gagacagtta taaaaggtag gcaaaagaaa ttcattattt actctatggg
    13021 aaattttatt tcaaatcagc ggcttgaaac cgtagatgat atctggactg agcgtggcct
    13081 attgatggac cttacttttg agaaaaaaga taacaaaaca aaaattaaaa cagttgaggc
    13141 ccatccaacc atggttttag ccaaaggtaa aggcattgtg ggtaaagaag gttttgaact
    13201 atacaattat cgaacaatgg ttttagaaga ttttatcaaa ggtggaaaat accacgacaa
    13261 gattgatgag gaaaccaaag caaaagtagc acttgcttat caggaaatta atgatttagt
    13321 taacctgaaa tggtaatgtt tggtggttta gcatctaacg ataaaaaata gagattaaac
    13381 ttaatttttc cttgataaaa agcagttatg gtgctaaaat agtaacgttg actaaatgca
    13441 catcctgtgc aaccgcgcga aaatcgttgt caagtagacc aagtctcacg attccaggcg
    13501 agtcttcaca gagggaaacc tcaaacaaaa attttatagg aggtgcatca tgagcacata
    13561 cgcaatcatc aaaactggtg gaaaacaagt taaagttgaa gtaggtcaag caatctacgt
    13621 tgagaaaatt gacgctgaag ctggcgcaga agtcactttt aacgaagttg ttcttgtagg
    13681 tggtgacaaa actgtagttg gtactccagt tgttgaagga gctactgtcg ttggaactgt
    13741 tgaaaaacaa ggaaaacaaa agaaagttgt aacattcaaa tacaaaccta aaaaaggtag
    13801 ccaccgtaaa caaggtcatc gtcaacctta cactaaagtt gtcatcaacg caatcaacgc
    13861 ttaatttact agcttatgat taaagcaatt tttactcgcc aaaaaaatgg tcaactgagc
    13921 agtgttacac tgacaggtca tgctggtagt ggtaaacatg gctttgatat tgtttgtgcc
    13981 tctgttagta ctctagccat taactttgtt aattctcttg aggtgttagc agattgccaa
    14041 gcattagtag atcttaatga tgttgaaggt gggtatatgg ctattacaat accaccacat
    14101 gacaacaagg aggaagttca attattattt gaatcttttc ttctaggaat gaccagtttg
    14161 gcgaaggact cttcgaaatt tgtcaacact caggtcatct aaatttaatt taagagagga
    14221 aacaacatta tgttaaaaat gaatcttgct aacttgcaac ttttcgccca caaaaaaggt
    14281 ggaggttcta catcaaacgg acgtgactca caagctaaac gccttggtgc taaagctgct
    14341 gacggtcaaa ctgtttcagg tggatcaatc ctttaccgtc aacgtggaac tcatatctac
    14401 ccaggtgtaa acgtaggccg tggtggagat gacacacttt ttgctaaagt tgaaggtgtt
    14461 gtacgtttcg aacgtaaagg acgcgataaa aaacaagttt ctgtttaccc agtagctaaa
    14521 taattgttta atatcgcatt atataaggct ttccaggtgt ctggaaagcc gattttttta
    14581 ttttgctact catttgctac ccatattcta aaagccgatg taggaagaga gcttgtcagc
    14641 agttttatct tttgaggatt tagtaacatg agcgtagata ttcatggtag tttggatagt
    14701 ttcacaacca agtgtattgg attttcttct atcctactaa agattgtagt tgcttattta
    14761 gagcaatagt tctaattgta aaaagtcagc ataataaaac aatatcataa gatgttgtta
    14821 taacgggctt tatggtataa ttaaggatac tcattttagg aaggaaagca atgaatattc
    14881 aacaattacg ctatgtggta gccatcgcta ataatggaac ttttcgagaa gctgcctcta
    14941 aattgtttgt cagtcagcct agcttatcag tttctatcaa agatttagag gcggaattag
    15001 ggtttcaaat ttttaatcgg actacttcag gaacagtctt gacaagtcaa ggactagttt
    15061 tttatgagaa agctttagaa gtagtcaaat cttttgattc ttttgaaaaa accttttctc
    15121 aagctgatct ggatcaaaat gaattttcta ttgctagcca acattatgac tttttgccac
    15181 ctcttattac agctttttcg caacaatacg atggtcatcg tgtctttcgt atttttgaat
    15241 caacaactat tcaaatttta gatgaagttg ctcaaggaaa cagtgaaatt ggtattattt
    15301 acttgaatgt agacaatcaa aaaggtctct ttcagcggat ggataagctg ggcttggaat
    15361 atgtttctct aattcctttc actactcata tttatctttc aaaaacgcat ccattggcta
    15421 atcgcgaagc gctttatttg aacgatattc aaggattacc tgcggtaagg tttacccaag
    15481 aaagggacga gtatttgtat tattctgaaa attttgtgga tactagtgaa tgccctcgta
    15541 tttataatgt gtcagatagg gcaaccttga acggtatttt agagagaacc aatgcctttg
    15601 cgacaggatc aggttttctc gatcatcgga gtgttaatgg gattaaagtg atacctctag
    15661 ctgatcatat tgacaatcaa atgatttatg tcaaacgaaa agataaaaac ctttctgttg
    15721 ctggggccac ttttgtgact attttaaaag actattttga taaaagaagg aaggccttaa
    15781 catgaaaaaa cgattgtttg tgcctagctt gatcctcctt gtagctttgg atcaacttag
    15841 taaattttgg attgtttctc atatagcgct tggagaagtg aaacccttta tcccaggtat
    15901 cgtcagcttg acttacttgc aaaacaatgg ggctgccttt tccatattgc aggaccagca
    15961 atggttcttt gttgtcataa cggttttagt tatcggttat gctatttatt accttgctac
    16021 tcatccccat ttaaatatct ggaagcaatt agctctcttg cttattattt ctggtggaat
    16081 cgggaatttt attgatcgtt tgcgtttagc ttacgtgatt gatatgattc atttagactt
    16141 tatggatttt gccattttta atgtggcaga ttcatacctt accgttggtg tcatattatt
    16201 agtgatatgt ttatggaaag aagaggatta tggaaattaa tgtgataaca tcggggcaac
    16261 gcttggataa ggcactagct gatttaagtc ccctgtcacg tggtcaagcc aatgaccaaa
    16321 ttaagcaagg ccttgtttta gttaatggtc agcaaaaaaa ggctaaatat acggttcaag
    16381 caggagatgt gatttgtttt gaactcccta aagaagaagt cttagagtac caagctcaga
    16441 atattccctt ggacattatc tatgaagatg atgctctcgc cattattaac aagccacagg
    16501 gaatggtagt tcacccttcg gcgggacacc cttcaggaac aatggttaat gccttgatgt
    16561 atcatatcaa ggatttgtcc tcaatcaatg gtgtggttcg tccaggaatt gttcatcgga
    16621 ttgataagga cacctcaggt ctcttaatgg tagctaaaac agatgcagct catcaagcct
    16681 tggcagaaga attaaaggct aaaaaatctc tcagaaaata cctcgccatt gtccatggaa
    16741 acctaccgaa tgatcgcggg atgattgaag ctcctatagg tcgtagcgaa aaggaccgta
    16801 aaaaacaggc tgttacagca aaaggcaaag aagcagtgac acgttttaca gtgctagaac
    16861 gttttggcga ttacagtcta gtggaattac aactggaaac tggtcgaacg caccaaattc
    16921 gtgttcatat ggcctatatt ggtcatcctg ttgctgggga ccctttatat ggaccacgta
    16981 agacactatc aggtcatggt caatttcttc atgcaaaaac attgggatta acgcatccta
    17041 tgacaggaaa agaaatgata tttactgttg aagctcccga aatttttcaa aaggtgttaa
    17101 agctgctaag aaaaaaataa acagcgagga gtagccatta tttttagtct tatatctttg
    17161 tgtaaagttt ttaacattat aaaaaatact aaaaagcatt tacgataagc caggaaatat
    17221 gagaagtctt ttatgttatt gggatatgca acatatctgt tagagatctt tatttaaact
    17281 ccttaaagaa aagagatatt ccaaatagat gctaggtatt gtctttttta cttgtttttc
    17341 ttttcttttt ttgctatact gattgaaata aaatattcct ttaaaacagt cctgtgaggc
    17401 tgtcaaggag acggattaaa aacatgcact atgatactta attgtgctat attttgctga
    17461 atctccttgt ttccaaggag atctttttat ttgaattttt agaggaggtt tcaacatgaa
    17521 aactaaggaa attgttgatg acgtgaccat gaaacgcgcc attacccgta ttacttatga
    17581 aattatcgaa cgaaacaaac aattggacaa tgtggttttg gcaggcatca aaacgcgagg
    17641 tgtttttcta gctcgtcgca ttcaagaaag actgcatcaa ttagaagggt tggatttacc
    17701 aattggtgag ttagacacca agccttttag agatgacatg agagttgagg aagacacaac
    17761 tctcatgtca gtagatatta caggcaagga tgtgattttg attgatgatg tgctttatac
    17821 tggtcgtacc atccgcgcag ccatcgataa cttagtgagt ttaggtcgac cagcccgtgt
    17881 tagtctagct gttttggtgg atcgtggcca tcgggaatta ccgattcgcg cggattacgt
    17941 tgggaaaaat attccaacaa gcagtgtaga agaaattgtt gttgaggttg tcgaagtcga
    18001 tggccgtgat cgtgttagca ttattgaccc aacataagaa agaggtttta ctgtgaaaga
    18061 tgtcagttat gatgtggaag aagtgccaaa ggcaggtatg ttagttggtt tgtcctttca
    18121 gcacttattt gctatgtttg gtgcgactgt tctggttccc attctagttg gaattgaccc
    18181 atcagttgct ttgttatcta gtggtttagg aacccttgcc cacttatcag tgaccaaatt
    18241 taaaattcca gcctacatgg gctcaagttt tgcttatata gcagctatgc agttactgat
    18301 gaagacaaat ggtattggtg ctgtagctca aggggctatg acaggaggtc tcgtttacct
    18361 gattgtagct ttgattgtta aagctattgg caatgattgg attgataaca tcttaccacc
    18421 gattgtggtt ggtccgattg ttatggtcat tggtctaagc ctagcttcta cagctgttaa
    18481 tgatgtcatg ctaaaaaatg ggaactataa tctgacttat cttgttattg ggttagtcac
    18541 cttattatca gttatttttt tcaatattta tggtaaagga attgttgcta ttgtaccaat
    18601 tttattgggg ctattagttg gttatgttgt agccctttta gtgggtgttt tcaccggtca
    18661 agaaattgtt gattttacca atgtggctca ggccaaatgg tttagtatcc catcagtgga
    18721 aattcctttc ttaacctacg gggttaaatt ctacccaagt gccattttaa ctatggcacc
    18781 cattgccttt gttacaatga cagaacattt tggacacatt atggtcttaa atagtttgac
    18841 aaaaagagat tatttcaagg atccaggact tgaaaagact ttaactggtg atggttttgc
    18901 gcaaatcatt gccggcttct taggggcacc tccggtcact tcttatggtg aaaacatcgg
    18961 tgtaatggct ttgaataaaa tattatcggt ctatgttatt gcaggtgccg cagtgattgc
    19021 cgctctcctc agttttatcg gtaaggtatc agccttaatt caatctattc caacaccagt
    19081 tattggaggt atatcagttg ccttgtttgg tgtaatagct tctagcggtt tgaaaatctt
    19141 gattgaatcc aaagttgata tggataataa gaaaaactta ttgattgcca gtgttatttt
    19201 ggtctctggg attggaggat tgatgcttca agtaaatggt cttcaaattt caggtgtcgc
    19261 tttctcaaca cttcttggca tcatcttata tcaggtactt cctgaaaaat aggaagaatt
    19321 gttgttagac cacttacata gtcatcagtc gttaatgata aaggggataa tcatgtcagt
    19381 tgtaaataat cgcgttgcac ttaccaattt agtttcaatg gaagcattaa ccaccgaaga
    19441 agttttaggt cttatcaacc gtggttcaga atataaggct ggtaaggttg ttatttcgga
    19501 tcatcaaaag gatcttgttg ccaatttatt ctttgaaaac tccacccgaa cccataagtc
    19561 ttttgaagtg gctgagaaga aactcggctt gacagttctt gatttcaatg ctgatgcaag
    19621 tgcggttaac aagggagaaa gtttgtatga tactgtgtta accatgagtg cacttggaac
    19681 agacatctgt gtgattcgtc acccagaaga tgattattac aaggaattag tagaaagtcc
    19741 aactattaca gcttccattg tcaatggagg agatggttct ggacaacacc cgagtcaatg
    19801 tttacttgac ttattaacca tctatgaaga atttggacgc tttgaaggct taaagattgc
    19861 gatcgctggt gacttaactc actctagggt ggcaaagtcc aacatgcaaa tcctcaaaag
    19921 attaggagca gagctttatt tctatggacc tgaagaatgg tattcagaag cctttaatgc
    19981 ttacggcact tatattgcta ttgaccagat tattaaggaa ttggatgttc ttatgttgct
    20041 gcgtgtacag cacgaacgtc atgacggcca tcagagtttt tcaaaagaag gttaccatca
    20101 agcgtttgga ttgacacagg aacgttacca acaattaaaa gattctgcta ttattatgca
    20161 tccagctcca gttaatcggg atgtggaaat tgcagatagt ttggtggaag ctcctaaagc
    20221 gagaattgta tctcagatgg ctaatggtgt ctttgttcgc atggctatta ttgaagccat
    20281 tttgaatggt agaaataaaa actcatgatg aaagattaaa gcatgtttag aaggtaggaa
    20341 aaggtaggaa aatgacaaaa cgattactta ttttagaaga cggcactatt tttgaaggag
    20401 aaccctttgg tgctgatatt gatgtgactg gtgaaattgt ctttaataca ggaatgacag
    20461 gctaccaaga atccatcact gaccaatcct acaatgggca aattttaacc tttacctatc
    20521 ctttgattgg aaattatggg attaatcgag atgactatga gtccatttca cccacctgta
    20581 aaggggttgt tgtgtctgaa gtcagtcggt tggcaagtaa ttggcgcaag caaatgacgc
    20641 tagatgcgtt cttgaaaata aaagggattc cagggatttc gggaattgat acgagagctt
    20701 tgaccaaaat tattcggcaa cacggtacca tgaaagctac gatggcagat gatggagata
    20761 gtatccagca tttaaaagat caactgcgtg caacagtctt accgactaac accattgaac
    20821 aagtgtcaac taaaacagct tatcctgcac cagggatcgg gaaaaatatt gtattggttg
    20881 attttggttt aaaacattct attttaagag aattttctaa aagacaatgt aatatcaccg
    20941 ttgtcccttt caacatcact gcagaagaag tgctccaact aaatccggat ggtttgatgc
    21001 tatctaatgg cccaggaaac ccagaagatt tgccagaggc tttagacatg attcgtgggg
    21061 tacaaggtaa gattcctatt tttggtattt gtatgggaca tcaactcttt agtctagcaa
    21121 atggtgccaa gacttgtaaa atgacctttg gtcatcgtgg ctttaaccat gccgttagag
    21181 aaattgcaac gggaagaatt gattttacca gtcaaaacca tggttatgcc gttgaacgta
    21241 gtagtctacc tgatacttta atggttacgc atgaagacat taacgataag acggtcgaag
    21301 gagtgaaaca tagagatttt ccagcttttt cagtccaatt tcaccctgat gcggctccag
    21361 gacctcatga tgccagctac ttatttgacg aatttttgga aatgattgat tcttggagat
    21421 gtacgagtaa atagtaaatc agttgcaatt tcacgactat atcacctctt gttactctat
    21481 ttattaacat ttacagttga tttagtaccc tatcaaaaat attatttaat gttgctctgt
    21541 gaggcaacaa atagagaaag gaaggcaagg agaacacaag taaataattt agtaaaaagt
    21601 taggttttca aagagataaa gctcttattg agaagtgctg actttaccgc ttcttgtggt
    21661 attcttggta tcattaatta tgccaaaacg aaaagatatt caaaaaatca tggttatcgg
    21721 gtctggcccg attattattg gacaagctgc tgaatttgat tatgcaggga ctcaggcctg
    21781 tttggctcta aaagaagaag gctataaggt catacttgtt aattcaaatc ctgcaacgat
    21841 tatgacagat aaagaaatcg ctgataaggt atatattgag cctttgactc ttgaatttgt
    21901 taatcgtatc attcgaaaag aaaggccaga tgccatactg ccaaccttag gtggtcaaac
    21961 gggcctcaat atggcaatgg cattatctaa agctggtatt cttgatgatt tagagattga
    22021 attgttaggt actaagttat ctgcgattga tcaggcagaa gacagagacc ttttcaaaca
    22081 attaatgcaa gaactagacc aacccattcc agaatctaca attgttaaaa cagttgatga
    22141 agctgttaca tttgctagag atatcggtta tccagtcatt gtgcgcccag cttttactct
    22201 tggtggaact ggtggaggaa tatgtagtag tgaagaagaa ctttgtgaaa ttactgaaaa
    22261 cggcctaaag ttatcaccgg tcacacagtg tttgattgaa cgctcaattg ctggcttcaa
    22321 agaaattgaa tacgaagtaa tgcgagatag tgctgataac gcgctggttg tttgtaatat
    22381 ggaaaacttt gatcccgtag ggattcacac aggagattct attgtatttg caccaacaca
    22441 gaccctttct gatattgaaa atcaaatgtt acgtgatgct agtttaaaaa taattcgagc
    22501 tctgaaaatt gaaggtggct gtaacgtcca gttagcacta gatccttata gtttcaaata
    22561 ttatgttatt gaagtaaatc ctagagtgtc tcgttcctct gcccttgctt caaaagcaac
    22621 aggttatccc attgccaaat tggctgcaaa gattgctgtt ggcctaacac ttgatgaaat
    22681 gattaaccct atcacaggca ctacttacgc tatgtttgaa cctacccttg attacgttgt
    22741 tgctaaaatt ccgcgttttc cgtttgataa atttgaacat ggtgaacgcc aactgggaac
    22801 tcaaatgaaa gcaacaggtg aagtcatggc tattggccgt aatttggaag agtccttact
    22861 aaaagcctgt cgttctttag aaattggtgt ttgccataat gagatgacaa gtttaagtaa
    22921 cattagtgat gaagagttag tgactaaagt gattaaagct caagatgatc gactttttta
    22981 cttgtcagaa gcaatacgac gaggttatag tatcgaagaa cttgagagct tgaccaagat
    23041 tgatttattt ttcttggata aattacttca tattgttgaa atagagcaag atctacaaat
    23101 gcatgttgag catttggaaa gcctcaaaaa agcaaagcgt tatggatttt cagatcaaaa
    23161 aattgcagaa atttggcaaa aggatgaaag tgatatcaga gcgatgagac attctcatag
    23221 cctgtatcct gtctataaaa tggtagatac ttgtgcagct gaatttgatg ctaaaacccc
    23281 ttatttttat tcaacatatg agttggaaaa tgaatctgtt cagtcgaata aagaatcaat
    23341 tttagtttta ggttcaggcc ctattcgtat tggtcaaggg gttgaatttg actacgcaac
    23401 ggttcattct gttaaagcca tccaaaaagc tggctatgaa gccattatca tgaattcaaa
    23461 tccggagact gtatctacag atttttcggt atcagataaa ttatattttg aaccgttaac
    23521 ctttgaagac gtcatgaatg tgatagattt agagcagcct aagggagtaa tagttcagtt
    23581 tggaggacaa actgctatta acttagcaca agcactttct gaggcgggtg ttacaatatt
    23641 aggaacacaa gtagaggatt tggatagggc tgaagacaga gatttatttg aaaaggcgtt
    23701 gaaagagctt ggaatcccac aaccacaagg tcaaacggca actaatgaag aagaagcttt
    23761 agaggcagct aaaaaaattg gttttccagt cttagttaga ccttcttacg tacttggtgg
    23821 ccgggctatg gaaattgttg aaaacaaaga agatcttaga gaatatataa gaacagcggt
    23881 taaagcaagt cctgaacatc ctatcttagt ggactcttat attttcggta aggagtgtga
    23941 agtggatgcc atttcagatg gaaaatcagt cttaattcct ggtattatgg agcacatcga
    24001 aagagcaggt gttcattcag gagattcaat ggcagtttat ccgccacagc aattatctaa
    24061 gcaaattcaa gaaacaatag ctgagtatac taaacgttta gccattgggt taaattgtat
    24121 tggtatgatg aatgttcaat ttgttattaa aaatgaacaa gtctatgtta ttgaagtgaa
    24181 tccacgcgct agtcgtaccg tacctttctt atcaaaagta acaggtattc cgatggctca
    24241 gattgctact aagttgattt taggtcaaac attaaaggat ctaggatatg aagatggtct
    24301 ttatccccaa agtcaactgg tgcatatcaa agcgcctgtg ttttcattta caaagttggc
    24361 acaggttgat agtcttttag gtcctgaaat gaaatcaact ggagaagtaa tgggttcgga
    24421 tactagtctt gaaaaagcac tttataaagc gtttgaagcc aataattctc atttatcaga
    24481 atttggtcaa attgtcttta ctatagcaga tgatagtaag gcagaagctc taagtctagc
    24541 aagacgattt aaggcgattg gctatcaaat tatggctact caaggtacag cagcttactt
    24601 tgctgagcaa ggcttaagcg cgtgtttagt tggaaaaatt ggtgatgctg ccaatgacat
    24661 cccgacactc gtgcgacatg gtcacgttca ggcaattgtt aatacagttg gaattaaacg
    24721 cacagctgat aaagacggac agatgattag aagttcagct attgaacaag gagttccttt
    24781 atttacagct ttagataccg caaaagctat gttaacggtt tt
//
LOCUS       DAUCWO010000022        24732 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUCWO010000022 DAUCWO010000000
VERSION     DAUCWO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24732
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.022"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            20..316
                     /locus_tag="VGY20_000830"
     CDS             20..316
                     /locus_tag="VGY20_000830"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4273698.1"
                     /translation="MNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVGRLLHQLGLYAKGSRYQYKYYNRRRSS"
     gene            392..589
                     /locus_tag="VGY20_000831"
     CDS             392..589
                     /locus_tag="VGY20_000831"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEQ4273699.1"
                     /translation="MPTQEGILYVSVFIDVYSRKVVGWAMGRRMQDKLVTEAFNQAYN
                     REKPKEGGIVHTDQGSQYTGA"
     gene            629..844
                     /locus_tag="VGY20_000832"
     CDS             629..844
                     /locus_tag="VGY20_000832"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4273700.1"
                     /translation="MSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYS
                     ETYYNPKRLHSALGYLSPVEFEKIVTH"
     gene            971..1126
                     /locus_tag="VGY20_000833"
                     /pseudo
     CDS             971..1126
                     /locus_tag="VGY20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1173..1703)
                     /locus_tag="VGY20_000834"
     CDS             complement(1173..1703)
                     /locus_tag="VGY20_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ4273701.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1696..3030)
                     /locus_tag="VGY20_000835"
     CDS             complement(1696..3030)
                     /locus_tag="VGY20_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEQ4273702.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLTEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDVPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
     gene            complement(3706..4173)
                     /gene="perR"
                     /locus_tag="VGY20_000836"
     CDS             complement(3706..4173)
                     /gene="perR"
                     /locus_tag="VGY20_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ4273703.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQPDF"
     gene            complement(4225..4680)
                     /locus_tag="VGY20_000837"
     CDS             complement(4225..4680)
                     /locus_tag="VGY20_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ4273704.1"
                     /translation="MIYSFQNPSEDVLKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            complement(4867..7509)
                     /gene="polA"
                     /locus_tag="VGY20_000838"
     CDS             complement(4867..7509)
                     /gene="polA"
                     /locus_tag="VGY20_000838"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ4273705.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(7640..9367)
                     /locus_tag="VGY20_000839"
     CDS             complement(7640..9367)
                     /locus_tag="VGY20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ4273706.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAIFLIAALGRGIMTMASGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMAAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(9383..10579)
                     /locus_tag="VGY20_000840"
     CDS             complement(9383..10579)
                     /locus_tag="VGY20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ4273707.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVRKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(11067..12158)
                     /gene="ulaG"
                     /locus_tag="VGY20_000841"
     CDS             complement(11067..12158)
                     /gene="ulaG"
                     /locus_tag="VGY20_000841"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ4273708.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSLEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(12411..14057)
                     /locus_tag="VGY20_000842"
     CDS             complement(12411..14057)
                     /locus_tag="VGY20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ4273709.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(14382..15086)
                     /locus_tag="VGY20_000843"
     CDS             complement(14382..15086)
                     /locus_tag="VGY20_000843"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ4273710.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGSVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(15088..15951)
                     /locus_tag="VGY20_000844"
     CDS             complement(15088..15951)
                     /locus_tag="VGY20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ4273711.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(15956..16618)
                     /locus_tag="VGY20_000845"
     CDS             complement(15956..16618)
                     /locus_tag="VGY20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ4273712.1"
                     /translation="MTHLPNLQVALDHSDLQGAIKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIRRI
                     WG"
     gene            complement(16727..17212)
                     /locus_tag="VGY20_000846"
     CDS             complement(16727..17212)
                     /locus_tag="VGY20_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4273713.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     S"
     gene            complement(17335..17613)
                     /locus_tag="VGY20_000847"
     CDS             complement(17335..17613)
                     /locus_tag="VGY20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4273714.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(17684..19117)
                     /locus_tag="VGY20_000848"
     CDS             complement(17684..19117)
                     /locus_tag="VGY20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4273715.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(19424..21925)
                     /locus_tag="VGY20_000849"
     CDS             complement(19424..21925)
                     /locus_tag="VGY20_000849"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ4273716.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSGFLDHLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(22136..23275)
                     /locus_tag="VGY20_000850"
     CDS             complement(22136..23275)
                     /locus_tag="VGY20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ4273717.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            23760..24041
                     /locus_tag="VGY20_000851"
     CDS             23760..24041
                     /locus_tag="VGY20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273718.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHQTVHAAEQALAGTCTYSNE
                     NPYVGVCFERKDGMPIEENIITKNNDFSLLNWFWEIVTNWWSKITGYFS"
     gene            24226..24393
                     /locus_tag="VGY20_000852"
     CDS             24226..24393
                     /locus_tag="VGY20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273719.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGEHQTVSANDANYSNGFYGHN
                     GPESWLLYTVW"
BASE COUNT         7428 a         5351 c         4587 g         7366 t
ORIGIN      
        1 gaaggaaaat agtgttagct tgaatagtca tcacgcctgt caaactcttc aagtctcccg
       61 ttcaggtttc tatgcttatt taaaacgtcg tccctcttca aggcatgttg agaatgaagc
      121 tttgaaggaa atgattaaag ccattttcta tgaacataaa gagcgttacg gaagtgtacg
      181 aattacccaa gaactttgta gacgtggcat ccatgtcaat cataaacgtg ttggtagact
      241 ccttcatcag ctaggtctct atgctaaagg aagtcgatat caatataaat actataaccg
      301 tcgacgttct tcatgaactc gtcccaatct tgttaatcaa tgtttccaag cgactggtaa
      361 gaataaatta tggctaggag acttaaccta cattcctaca caagaaggaa tactttatgt
      421 gtctgtcttt atagacgttt atagtagaaa ggttgttggt tgggcaatgg gccgacgtat
      481 gcaagacaag ttggtaacag aagctttcaa tcaagcttat aatagagaaa agcctaaaga
      541 agggggaatc gtccatacag atcagggttc tcaatatacc ggagcatgat ttcaggacct
      601 gcttagatgg aaaaaatgta agtccaggat gagtcgaaaa ggtaatccat acgataatgc
      661 actcatggaa tccttttata aaacattaaa aagagagctt gtcaatgatg ctcatttcgc
      721 aactattgag caagctcagc ttgagatttt taaatatagt gagacttact ataaccctaa
      781 acgtctgcat tcagctttag gatatctctc acctgtagaa tttgaaaaaa tagttactca
      841 ttagcttaac tttatgtcta ttttttcttg acaagtccag ggtatgaagt ccccttatac
      901 acatactttc aaaacagctc tcaatcctct gtccgctaca aaaatacgtc accgctgccc
      961 tccggctgct tcattcaacg acaaacttga agccacaaac agactcatca agggcattaa
     1021 gtacaatgcc tttagatttc agaactttga caactttaaa accaaaatcc tcatcgcttt
     1081 gaacatccaa aaagagagaa ccaatctgat tctctctcgt tgttaatgat aagtcaccca
     1141 ctacagttga caatgagtct cttacttcct gtttactcat ttaatagctc ttctggttcc
     1201 caagattgat taaactcatg gtccttaaaa agagaagcca atcctgttat ttgtttcagt
     1261 cttaaaattt catctttatc cattcctaaa tgttttgcaa tccagttatc acttcgccca
     1321 cactcatgaa gatcctttac aatctgactc atcaaatcaa catcatgact tcctctagct
     1381 ctattatgtc gaatggtaga agccattcga tagtctaatg acttatcaat aacagaaaca
     1441 ggtaatctcc cactttccct ttcataaata tctgaataat ctagcattat acgatatcta
     1501 tgaaaaccat caacaatact ataccgatcc tcttccttat cgtaatagca aacaatcggc
     1561 atagtataac catcagattt tatactatca tacaataatt tcatttcagg cggagcaact
     1621 gaattcggat tataatcatt tggagaaatc ttttcaattg gaattgattt aatctcatag
     1681 actggactaa taaaattact cactttctaa ctcctttaaa ctcgtcttat atttctcttt
     1741 taaaaaggct actatttttt gttgctctct tgtcaaacca aaccccatag ttcgacaaat
     1801 atgatcattt tttaaaatac agaaacacat tcgtttccag cttggtacat cctttgttgt
     1861 tacaatatcg tctgtatcat caggaatttg atctaaaaaa ataacccttg aatgtttaaa
     1921 agaggtataa tttgaaatac catttctagc aatacgatat cctttttcaa ttagttcatt
     1981 aattgtttcc tccgtaagtc ctccgcctgt tttatgccaa aagtctattg atttattaaa
     2041 ttttctaaca taattatttc ttaacctcac tggtagcgtt gataaaagaa actgagtata
     2101 cgacttccac gaatgaccct ttggtaaagc aattgattta tatcccattg ctttagtacg
     2161 tccataaata ttcgaaaagt taactccttg aacacgtccc aaaagcttta cccaagtttc
     2221 ttgatctatt attctataaa gatttaggct atcaacagca tagtcttgaa aaggagatgc
     2281 aactctcatt tgtgatggtt ttaaacccgc tttatagaat agatcataaa gttcgttata
     2341 agaataagaa aacttatagt aagcatgcca tatgtcttcc actgaccaat catataaggg
     2401 agatgctgtc cagacatctt tgaattgttt ggtaatccag cattcctttt gataaccata
     2461 ctttttattg ataaaaccac tatatctgtg taaggattct gaagcacggt ttcctaatat
     2521 acaaactgtt ttttgatttc cgtgaatttg tttataccac ctaccaaatt gtttcgctaa
     2581 atcctcctga ttcattttat aacgataagt agtaattgaa ttattttcca aattaataac
     2641 ataatcttga gatggcattg gtcttaccca gatatcttct gttttgtcat cccacggata
     2701 ccaaaacatt tcataactac taagtgcagt tctagtagcc atcggtaagc acacccaata
     2761 caaagaaact ttttttctac cttctaaaga agtgaatgtt tcttgaacat attttgttgt
     2821 taaactgtac tgtgcttcaa aatcttgatg aaacacacct atttctctat ccggataata
     2881 tttatctcta aaatccagta aaatattaag aagtaaccca ctatcttttc cacctgagaa
     2941 ggaaatatag acaataggaa attcattaaa caaaaaatgt aatctttcta tcaatgcatc
     3001 ataaacattt tgatttaaat actttttcac taaacttcct cattatgcat ttaaaaatat
     3061 taatacatag attcactaag aatctctttt tcgatctttt tgttttttaa tttttcaagt
     3121 tgaataatta aaccttgctt ttctttttca aggtaataga ctctactcct tgtaagtcta
     3181 acttattctt aatcttattt atcgctcatt ttttaaattc cgatgctctc acagatatta
     3241 tcattgttta gttacatgtc tgtcaatcta ttcgccacca cactaggggc catctgattg
     3301 aaaaataaaa tttcggagtc cgttgtttgc tcaagaatca tatctgtcac aattacgtca
     3361 tactctgaag acagtgaacc atatcaacca tggtcagcct caagtcatca aagttgacaa
     3421 tctcaaaaaa tcctccaaaa tactctttta agaagttgcc aacattgtta taacttctct
     3481 caaccactaa tagtttcaag cgacgacgcg attgatttgt aagaaagaga ttttcccagg
     3541 tgaggaacaa acaatagagg agatgattga tggttctttc ctttaattcc ttatgatccc
     3601 cagcaaataa ctctttaaca taagcaacaa aggtctgata gtgaactggg ggtttttgat
     3661 ttgtaaaggc actatattta taatgccttt atttaagggt taactctaaa aatcaggttg
     3721 atcttttgct tggcaatccg gacaaatccc gtaagcaata actgggatac gcgtgacctt
     3781 ataacctgtt tgctcatggg cttctttggc aatatccata acatcaacat ccataaagtc
     3841 agcaatcttc ccacatattt cacaaaccac attgacgtgt tgatggccca taaagtcata
     3901 gtaggtggtt aaatcattgc tgattttcaa ctctgagaca aaaccttcat caactaaaac
     3961 tttcaaatta ttatagactg tggcaaggct catgttagga aagttaggct gtaagtcacg
     4021 gtaaatctta tcggcactag ggtgttctgt ggactgaatc atgtaagaaa taatggcttt
     4081 acgtgtctct gtaattcgaa tatgtttttc ccgtagatgc tctaggacat tttcataggc
     4141 atctagggct tgctgatgtg aatgaatgtc cataagctgc tactcctatc cttttttcct
     4201 ttattatcac gcacgtctct agtttcaatg attcgtgatg cattctggtt tagcgactaa
     4261 ttgcaaatag tctattttta ggcagcggtt catcacaata gcctctttgc ctgccgaccg
     4321 taaaatggtt tgtgcttctt ggttttcaag gcctaattgc gcccaaaaga ctttggcctg
     4381 acctgctaaa aagtctctgg caacttctgg taaaaactca cttctgcgaa agacatctac
     4441 aatgtcaacc tcaaagggaa ttgccttgat actggcatag accttctctc ccaaaataag
     4501 ctgacctgct aatttgggat tgactggaat gatccgataa tccatagcct gcataaattt
     4561 ggcgacacca taagctgctg tgtctttgcg gtcagaaaga ccaacaaccg caatggtttt
     4621 ggctgactcc aagtaagctt ttaggacatc ctctgaggga ttttgaaacg aataaatcac
     4681 ttgttcccct atctatttaa taacctctac agctgttcaa tatagcagga taactcctaa
     4741 agcatggctt caatcacttt taaccttatc cgtcaaaaag ctcaaagcag cttcatgact
     4801 tctcctacaa ttttttagtt tattataaca taagttgtca aaatgagaaa taacaagtaa
     4861 gaaaacttat ttggcttcgt accagctctg cccagtactt tcatcgacac ataaaggaac
     4921 agctaaatca acagcagctt ccatggtgtc tttgacaagt ttttttatag cagtcaactc
     4981 atcatttggc acttccaaaa cgatttcatc atgcacttga agcagcattt tagctctaaa
     5041 accacctgct tgcaaggcct tatccagatt aatcatagca atttttagaa tatcagctgc
     5101 gcttccttga atgggcgagt tgatagctgt acgctccgca aaagagcgaa cgttgaaatt
     5161 acgagagttg atatccggta attcacgacg acgcttaaag agggtctcta catagccctt
     5221 gtctttagcc tcacgcacca cattttccat ataggcttta atcccaggat agcgttcaaa
     5281 ataggtatca atgtaagact tggcctgttt acgggtgata ccaaggttat ttgacaaacc
     5341 aaaatcagaa atcccataca caattccaaa gttaacagcc ttggcattac ggcggtcatt
     5401 ggcagtaaca tctgctgcct tgtcaatccc aaacacacgc atagccgttg aggtatgaat
     5461 gtctgcccct tcattaaagg cagcaatcaa atgctcatcc cctgaaatat gtgccagtac
     5521 ccgcaattca atctgtgagt aatctgaact caacaagacg gcatcttcat gagatggggt
     5581 aaaggcctta cgaatcaaac gcccctgttc caaacgaata ggaatgtttt gaagattagg
     5641 gtccacacta gacaaacgcc ctgtctgggt gagatcctgc acatagcggg tatgaatctt
     5701 gccatcagcc agaatataat cctgtaaacc aatcacataa gtggattgca atttagtaat
     5761 ctggcggtaa tccaaaattt tagccacaat tggagcaatc ggtgccaaac gttccaaaac
     5821 atccactgct gtagaataac ctgttttggt cttcttagtc atctcaagag gtagttgcat
     5881 cttctcaaac aagatactgc ccagctgttt aggcgaatta atgttaaatt cttggccagc
     5941 catatcataa atctcttggg tgagcgcttc aataatgacc ttgttttgct ctgccatatc
     6001 ttgcaaagtc gccctattaa ccttgatccc ttcaatttcc attttagcaa ggacattagc
     6061 caaaggcaat tcaatattct gatagaggtc tagttgctca tgggcagtga gcttgtctag
     6121 catttgagac ttgctatcta ataatacttt gaccttacga gctaagtgtt ctaataagac
     6181 gtctttatca ggaacagccc gtttagcacc tttaccatag actgtatcgt cttcttccaa
     6241 agagatgtca gtaaaaatgc gggcaatggt gctaagctca ttgtcttcta cagttgacag
     6301 aaggtaatta gctaatctag catcatagct aggtgctacc aactcaattc ctaagtggct
     6361 caacaaaacc ttgctacgtt taaaatcata agtggcaatg ggtttttgga agacctgctt
     6421 aaaactatcc gtcgctaata ggctaaggtc agtggatgcg taaatctgct caccatgtcc
     6481 ccaagcaaaa ccaatgatgg cctcacgatg gtaattgtcc cgaagcgttt caaagtagaa
     6541 gatgtcttct gcgctaaaca aatcagcaga cacattggtc acttcttgat aggaaatggc
     6601 agctactggc tcttgaggca tctggctagc aagacctttc ttgagttgca caaaatccat
     6661 ctcatcgtaa aattgagaga ggctagcaac atctggacca ttgtagacga tatcatctaa
     6721 gccaattgtg ataggagatg ctgtattgat agtggcgagc gttttagata ggaaagcttg
     6781 gtcacggtca ttaatcaagt tttctttcat ttttgaggcc ttgaatccat caatatgctc
     6841 gtaaatgcct tccaaactac caaattcatg aaggagctta aggcctgttt tttctccaat
     6901 ttttgtcact ccaggaatgt tatccgattt atctcccatc aaggctttga ggtcaataaa
     6961 ctggtttggc gtcaagccca ttttttccat gaggtaagcc ggtgtgaatt cttcaaattc
     7021 ggcaacccct tttttagaaa tttcaaccac ggtattttcg tcagttaact ggatcaaatc
     7081 tttatcacca ctgacaatgg tcacatcaaa aggcacttct gtgcgctccg ccattttatc
     7141 aagcgtcccg ataatatcgt ctgcttcgta gtgttccaac tcataataag caatcccaag
     7201 ggctgtcaac atctcacgaa tataaggaaa ctgctcacga aattcatctg gggttttagc
     7261 tcgacccgcc ttataatcgg catacatttc tgttcggaag gtggttttgc ccgcatcaaa
     7321 agccaccagc acgtgagtag gctggactct tttcatcatg tggtctagca tcagatgaaa
     7381 gccataaatg gcattggtat gaagaccgct atggttttta aagcggtcaa tttgattgta
     7441 aagggcaaaa aaggcacgaa aggcaacaga tgacccatca attaatagta atttattctt
     7501 gttttccata agcttattat aacacagagg gacgggcttt tctcctttaa gccattggtt
     7561 ttgctgataa gtaaatggtt aacctaaaaa gcctaggaaa attcctagac ttctctattt
     7621 gctatttaga caaaagcact cattttgtcc aacttgatac tttagatttg tttgcctcta
     7681 cccatttttt ggcggcagct tctggactca tgcctttatt gatgtcaagc atgactgctg
     7741 ccatatcttt ttgggtccag tggaacttat caatgatttt gtagacattt ggaagatctt
     7801 ttttcaagcc tttacgagca atggtattga tgttctcagt cgagccaaag gtttctttag
     7861 gatctttaag gtacttcaag tcatacttgg caaacatcca gtgtggtgac caagctgtca
     7921 cgacaatagg atcttttttc ttgatcgctt gatccaagga agtcgtcatg gctcctgttg
     7981 atgccgccac taactcccaa ctagatagat tatgatactc ttttagagtc tttttggctg
     8041 ctgccatgat acctgcacct ggttcgatcc ctgtgatttt ttgatcggct tgtttgctta
     8101 agtcctcaat actattgaca tctgtcatgt attttggaac agctaaacca agtttggtcc
     8161 cttttaggtt aggtcctaaa tcatctaatt ttgacttgta tttctgatat tgttggccat
     8221 gggtgactgg caaccaagca ctagttgaaa agtcagcgtt gccattagca acggtttgcc
     8281 acatgacagc attgtcaagc ggagtcaagg tcacatggta accttcgttt ttcaaaactt
     8341 ctgcaatcac atgagttgag gcaacctcag aatcccactg cacgtaagcg atgttaacag
     8401 tttcaccctt atctgccata cccgaagcca tagtcataat accacgtcct aaagcagcta
     8461 tcaaaaagat agcaagggct gctagaccaa tccatttatt ggttttgcca gctttggctt
     8521 tttcttgagg tttactattg aacaactgtg tcatacggtc aagcacaatc gccaagataa
     8581 ccaaggcaag accactgaca aatccactac caatatcagc gtgttgaagg gctgacaaga
     8641 cttcacgtcc taaaccagga gcaccaatca tggagcctgt tactaccatg gaaagggcta
     8701 acatcatggt ctgattaaca cctgccataa tagtattttt agctaaaggc aattcaactt
     8761 taaagagctt ttgtttccca gttgacccaa aggcatctga ggcctcaatc aattctgttg
     8821 gaatgtcacg aatggccaaa tttgtaaaac gaactgttgg gggcaaggca aagatgactg
     8881 aagcaaatac tccaggcacc ataccaatac caaaaaaggc aacggcagga atcaagtaaa
     8941 caaaagccgg catggtttgc ataaagtcca aaataggatt cacaatctga cggacagtgg
     9001 catttttagc catccaaatc cccagcggaa taccaataag taccgaaatc aaactagcta
     9061 ctaacaccag agtaaaggta ttcattagct gcttccataa cccttgattg taaataaaga
     9121 gcaatcctaa caaggtaaag gttggcaaag gccatttttt cttagcaagg aaaaacatcc
     9181 ctgctgttac tagaacaata aaaagtaacg ggtggataaa taaaagtgtt ttggtcatcc
     9241 aatccatcaa aaagctacca actacttgca taatatcaaa tagacctgag aaagttttag
     9301 ttagccactc cgttagctgc tccaccagtt gagctactgg taattttgtt tgtaatatcg
     9361 tttccaaaat gcatcactcc ttttagttaa ggtcttcgtc tgagatatta gccaaggctt
     9421 caatcactcg tcctcgaata atgacaccta acagacgatt attatcatct gtcactgcaa
     9481 tcggagcaga tgaatcatag ataagaggca aaatatctgt aataatagta tcttttgaga
     9541 cagttctcac atcgcgatca atcacttctg atagcggtaa cccttttttg cgcgcctcta
     9601 tagcggcatc ggccgtcaaa ctaccgacta attggcggcg gcgattcgtc gccatcaaca
     9661 tagacacctc ttcgttgtgc atacggttca aggcaacttg aggtccatct aattcaacag
     9721 tagttgttaa cggtttgatc atgatatttt gtgctgtcaa gactttagaa cggtccacat
     9781 cctcaacgaa ttcacgcaca aagtcattgg ctgggttagt caagatttcc tcaccagtac
     9841 caatttgcat aatttgtccg tctttcatca aagcaatccg atcaccaata cgcaaagctt
     9901 cattcaagtc atgactgata aagatgatgg tttgtttcat gctatcttgc aaatcaagta
     9961 attcatcttg catctcacga cgaatcaaag gatcaagcgc tgagaatgcc tcatccatta
    10021 agagaatttt aggactatta gctagcgcac gggctaggcc gacacgctgc tgcatcccac
    10081 cagatagttg gtttgggtac tggtctttaa aatctaatag gcctgaatta tcaagggctt
    10141 tttctgccaa tcgctggcgt tcttctttgc gaacgccacg taattccaaa ccaaattcgg
    10201 tattttccaa aatggtttta tgaggaaaga gggcaaagct ttggaaaacc atgttaatgt
    10261 catggcggcg cacctcacgc agctgatctg ctgacatggt tgagatgtct ttaccttcca
    10321 gcaagataga tcctgccgaa ggttcaatca aacgatttag catgcggaca agggttgatt
    10381 tcccacttcc tgatagcccc ataataacaa agatttcacc ttttttgacc tcaaaactag
    10441 cgtcatagac acctacagta gcgcctgttt tcttaaaaat ctcgctctta tttttgccag
    10501 tctttaccat ctcaagagct gctttttgtt ttttgccaaa aattttactg agatgtttga
    10561 cttctaaaat tgtttccatt cgttttttct cctaggtaat cgtttatcat gtgaaatagt
    10621 gacatagctt tggcgaaata ttagccaata tttaacaaag tagctctaca caattaataa
    10681 acgtgtggtc acttttttac ttaaaaaaag aatctgccga aacagaacat aactatatta
    10741 tcacgtgtgg tcactttttg tcaaggacaa taactcgatc tggtaacgcc acaatgatag
    10801 gtgatcgctt tcattctttt aggtatggtc ttttaaccag tacaattttt tatcacaact
    10861 gatgactatt tctttgctga ttaggctatt gttacattag aaatccttct tttacctata
    10921 atatctcttt tgcggccatc gtaaataacg accacggtga ctcttaaaca tttgctcatc
    10981 tctccaaaaa aactcaccta agacagtgtc ctacgaagcg agttagctta atcaggcact
    11041 atcatcttag gtgagtaggg tgttgcttat agtaaagctt taaattggat attagaatct
    11101 tctaagaagc agtcatcaaa tccacgagga tgatggtatt cgatacgatt ttggtcttgt
    11161 ggataggtgt acttgccacc aacctcccag ataaagggat ggaaatcgta ttggagacgt
    11221 tctttgcgca ttttccacag ttcaaggatt tcatctgttg atgccataaa gtttgaccaa
    11281 atgtcataat gaactgggat aacgacttta gcacggagat tttctgccat acgaagaagg
    11341 tcaacagaag tcatcttgtc ttgaatccca attgggtttt ccccatagtt atttaacaca
    11401 acgtcaatat cataatcacg tccatgttta gcaaagtagt tcgaaaaatg agaatccgca
    11461 ccatgataga tggtaccacc tggtgtttca aaaatatagt taacagcttt gcgagccatg
    11521 tcatcatctg tgatagctaa acctgctaaa tcaccatctt gagcatcagc accttgtatt
    11581 gggagagtta ccagacaggt gcggtcaaac gactctacag cggtgatctt aatatctttg
    11641 aattcaaggc tatcacctgg ctttaagatc ataatacgat cttttggcac accccaattt
    11701 ttccaaactt caccacattc ataaggtcct acaaatttaa catgattaag ttttgggtta
    11761 ttgataatgg ctgccgcagt attgatatcg atgtggtcac tatgataatg agaaaccaaa
    11821 taataatcca actcattaat cataaatgga tcaatcacca ttggttgggc cctaagattg
    11881 ggctgtaatt tacgagctcc tgccatatta gccatttgat gtccacgaac catatcctta
    11941 acttgcttgg tcgctttccc acgatttgac cataagtcca tgacaacatt agcaccgcct
    12001 ggtgtcttaa tccagatacc acagtttcct aaccaccaca tcgcaaagtt gtcagctggc
    12061 acgacttctt gctcgatttc ttcatttaac catgttcccc attctggaaa cgtgttaagg
    12121 atccatgatt cacgtgtaat atcttgtact ttagtcatta gggtttcctc cgaatttgtt
    12181 cttcgtttct atactttaag tatagacttt cttgtcaatg ataaaaagac caagtgtcac
    12241 acagatctgt gttcaaaagt ggactgactt tttcattagc tcagtctaat actgtctcat
    12301 tttgcacact gtatctcgca tcaatactgt attcaaagct atattaaaaa cctcagttat
    12361 atttttctat gtcatagaaa actgaggtac tttgacgact taacacttac tcatagcagc
    12421 tcgcgatgaa tgagtttttc aattttagaa accaaggcat aacgatctga ttcatctttt
    12481 acaacagctt caatagcttt tgtgagctct gtagaaaagc gggagtgctc actcaagcgt
    12541 ccttgctttg aaaagcgtat caatttgatg atatcatcgt cacttaaaat agggttaaca
    12601 ataagcatcg ggattttagt ctttagcgta tctgtagtag caacaatcat atcgactgct
    12661 aaaaggtcgt aaacactctg atactgttct gtcgtaaaaa cagcttcaat ctgaccattt
    12721 gctagatacc tttgacactg tttgaacaat aacttttgaa tgcctatccc atcatctgaa
    12781 acaataacga gtttggtctt ttcttgctct gtattattgt gacgtagctc tcccccaaga
    12841 tggatagtca agtaggcaat atcatcgtct gtcaagctaa tcgtccaatc ttgttccaaa
    12901 atagtggcac aagattgagt catagcaaac aattcttcgt atttttcttt aatatggtct
    12961 gttaagggat taactaaaaa aatcccataa gctttacggt aaacgagcgc cttacagtgg
    13021 gtcgtcaact gcttcagcaa atcctgttta tgcgtgaaat gtaactgata acggctctcc
    13081 aactggtcaa taaagtgact aatggttgct cgcatgtcgt cataatcttg actttccacg
    13141 tgatgatctt gatcttttcg aaaagacaac atcaacatcg ctaccatact aacttcgata
    13201 tcatccaaat gaagcttaaa attatggtaa agctctctag ctaaatcctg agcaatgtga
    13261 tattctttac gtttccaaat caaatgaaat tcttgtttaa gagcagactt tgtttctgaa
    13321 tcaagacgca tattacgata acttaaaagc atgaaaggta aaatctgcaa catgaattgg
    13381 ctatcttggg tattaattgt ctttcctaag ctagcttgag ataaaaagag atactcatga
    13441 aaataagtca aaatatcttt agaaaaataa accgataagc cttgtatctc tgagagttta
    13501 cggttgaata tatcaataaa gctagtattg ccaccttggt aaacgtccac taacaatttg
    13561 tacaggtatt gaataagagc cattggatga caaccaaaat aatatcccct agcctttgtc
    13621 gcatgaagct gaatcttgta ttgtttgtct tccagttctt cccttaactc agtcaggtca
    13681 tttaaaatcg tgttacgaga cacatcattg atctgcatca gtttatcaat ggtcacgcgc
    13741 tcagtggaaa ccgcaatata aatcgaggat aggcgtctgc gctcatcact tttcatgaca
    13801 taattataat cagtcacttc ttctaataat aagcgacaag cagctttttg atcagctgtt
    13861 aaaagaatgc caagtctagg ataagagata atctgatcta ctccctttgg caaagcctga
    13921 ttaattttgt caagctggta ataaacttta cgacgtgact gatttaaagc atgagaaatg
    13981 gccataactg tctcaggtgt ttctagttta agtaaataag acagcaagtc ataactcttt
    14041 ttatctaaaa tcatcatgtt tacaaacctc ctctctaaaa atccatcctt gcttatttta
    14101 tcatgatttc agtgactgcg cagccaagtt ttgaaaaact caccataata agattcgctt
    14161 aatatgattc gagctaatca cctcaactga tccaacacca acaccttata tctggtctct
    14221 aagaactcca caacatacga ttcatccagt ttgtgctcat tggcgtttaa acagttcatc
    14281 gaaaaggaca agtcaaaagg acttgcccaa acaatgatta acaaaacctt gaactttctg
    14341 gcttctagtg agaacctgtg ctaataagcc atatcgcgac ctcatttttg accgtaataa
    14401 gcatttgggc catgtttacg caaataatgc ttatccataa tgtatcttgg cgctggctct
    14461 acgcgtggat tgatttgttc tgtcaagcga ttcattttag ccacttcttc taagacaacc
    14521 gagtggtaaa cggcttgctc tggggttttc ccccacgtaa atgggccatg attacgcacc
    14581 acgattcctg gaaccgccat cgggtcaaga cctcgcttgc taaactcttc taaaatcacg
    14641 ctaccagttt cttgctcgta agccccatca acttctgctt ttgtgagaga gcgagcacaa
    14701 ggaaccgggc cataaaaata gtcagcatgg gtagtcccat aaaacggaat atcacgacct
    14761 gcctgtgccc agccgacagc ttctgtggag tgagtgtgga caataccgcc cacttctggc
    14821 caagctttat aaagttccac atgggttggc aaatctgaag aagggttcaa atcaccttcg
    14881 accacattcc catcaaggtc tgtcaccacc atattttctg gtgtcaagag gtcgtagtcc
    14941 accccagaag gcttgatgac aatacgtcct aattcacgac aaacttcaga gacattgccc
    15001 cacgtaaatt tgacaagacc gtgttgtggt agagacttgt tagccgcaca gacacgttca
    15061 cgcatttctt gcaagttttt cgccatatta tgccaacccc gctttctcaa taagtgggta
    15121 aaggaagtcc tgagcttctt tgatagcagc ttttgtttcc tccaccgtgt cacaattttc
    15181 tgaccacatt tcaatcaaaa aaggcccatt gtagttggtt ttcttgagga ccgcaaacag
    15241 ctcttgccag tccacacaac cttgaccaaa tggcacatca cgaaactgcc cttttgaggt
    15301 ttccgtgaca gcataagtat ctttcaagtg gagggctgca atggacttgt gaccgttgta
    15361 aaattcactc cacaaatcat tatgccaggc agaaacattg cctgcatcag gataaacaaa
    15421 gagataaggc gagtcaatct ctttttcaac tgccaagtat ttttcaatag agttgataaa
    15481 agggtcatcc ataatctcaa tagaaagcat cacttgggct tcttcggccc agtcacaaga
    15541 ttgtctcaag tttttgataa aacgagcacg ggtttcaggc gatttttctt catagtaaac
    15601 gtcataacca gctaattgaa tggtacgaac gcctaagtcc tgtgccaatt caatacattg
    15661 tttcatcagt ttaagggact tggcttcaat agcaggatca tttgagccta gtgggtaacg
    15721 tctatgacca gaaaaacaaa tggtcgggat gcgaatgcct gtttcataaa tggctttcac
    15781 taaatcaagc cgttcttcct tagtccactc taagcgcgct aagcgagcat ctgattcatc
    15841 aacagacatt tccacaaaat caaaacctaa gtcttttgca aattgcaagc gttcacgcca
    15901 ggtaaactgt tttggcgttg ctttttcata aataccaata ggacgcgcca taaagttacc
    15961 cccagatacg tctgatttca tctttgaagg cacgcgccgc tgcagcagga tcttctgctt
    16021 cagtaatgcc tcgccctgcg ataaaggtaa agacatcaac cccttcaaaa agtttcaaag
    16081 tgtctacatc aagcccacca gttactgaca cgcggaagcc catgtcaatc aatgttttca
    16141 ccttgttaag gtctttttca ccccaagttt ctccagcaag aagggcatca cgagattggt
    16201 ggtaaatcgc ttgggaaatc ccagcgtcaa gccataattg agcttgctca taagtccaat
    16261 caccataaag ttcgatttga atttccccac ggtcaccgcg ctcttctttc atagccttga
    16321 gggctgcttc catggttgga atcgtcgcac agcaaatgca ggtcatccag tcagcaccac
    16381 gttttgcatt gtttttagcg accgtaccgc cagcgtcagc acatttggta tctgctacga
    16441 taattttctc tgggaagagg ctgcgtaata cctcaactag ttcgcttccc acttgaagca
    16501 aacaaacggt accagcttca atcacatcta cttcgtgacc cacagcaacg gctgctttga
    16561 tagccccctg caagtcagaa tggtctaagg caacttgtaa atttggaaga tgtgtcatgt
    16621 gtttttccta gaactctcag cggctatgaa aaagctgatg aggagttctt atcctttcaa
    16681 taatgattgt gagtcgtttt atccgtttaa gtcagtaagc tatcaattaa gagtctaaat
    16741 ccaaaccttc taaatacggg ctattttttg attcttctac catttctaga acctctttag
    16801 gactttggca ggccaccaag cgttcaatgg cgttatctag ttcaaacaag gccacaattt
    16861 gtgggatagc cactgttgtg tgaatagaag ggtctgtagc ggccaacgtc aaaagaacag
    16921 aaacctcttt accatctgag aaggtaactg gttttgttag tgtaatcaaa gcaaatgcat
    16981 tgcgattaac gcctaaacct gcttctgcat ggggcatagc catacctggc atcaacacat
    17041 aataagggcc gtatttttct gttgatgcaa tgatggcgtc gtagtaagct gacgtcactg
    17101 ctttgctatc aatcaaaggt tgaacagcta attgaacagc ctcttgccaa gtatcagcac
    17161 ttagccccaa tcggatggag ttgttgtcaa taaaggcttg ttttaaattc ataaaaacta
    17221 tctctttcta gtgtcttgct ttgcaacaca caccattttt actagtttta tcctactaaa
    17281 gagaaagagc tgaaaacctt cgtcacaact ccttcgtttt gattcagaat tagcttatag
    17341 cacttcttgt agtttggttt taatttcatt atcatccatc aggttatcaa gaccaaccaa
    17401 gtggcctttg gtacgaccat caagctcatg aatcaagtgg tttgatgcca ctacaatatc
    17461 atagccagaa gctagacctt tagcttcacc aactgagcaa gaagctgact gaatatctgt
    17521 cacaccaagt tgacgaagcg cattttcaac tttcattttg atcaccatag atgaccccat
    17581 accattgcca catgctgtta agactttaac cattgttttt tcctccgaaa atgttttatt
    17641 attgttttgt taaaatagca gagctatttc ttaagatgca gtgttattga gcttccccac
    17701 ggtagtaagc ttctttgtcc ttagcttttg cgaactgcaa ttgtggaatg gcaagtaaga
    17761 agatgcaaac aagcacatac cctgcaatac caaggaattt gaagaggtac ccaaatggta
    17821 accatggaag aaccaagtcg atattaccgt gataaccacc tgtaagtcct aaaagaccta
    17881 cagcaacagc accaagtgcc acctgaataa tacctgagat aaatgataga gctaccgctg
    17941 ctttccagcc accacgttta tcagcataaa ccgcaattgc cgcattatca aagaatacgg
    18001 gaacgaatcc tgtgataatt aagattggat ttttaaagat aacaagaaga gcaatggtga
    18061 ttaattgacc aatcaaacca aaggcaaatc ctgaaagcac cgcatttgaa gaaccaaagc
    18121 cataggaagc tgcaacgtca accgctggga atgaccctgg aagcaatttg cttgagatcc
    18181 cttgaaaggc attggtcaat tcagaaacaa acatgcgaac accctgcatc aagataaaga
    18241 gataaactga gaaggtgaga gaggtttgca aaatatacat gaagaaagct tgcttagctg
    18301 ggttgaatgc tccaggtccg attaagtcca catcagacat aatgtctgga ccaaggacag
    18361 ccaagatagc tccgaaaaag accaacatca aagttgctga tgcaacaaca gtatcatgga
    18421 aaatgtttaa gaaggttggt aattttaggt tatcaaggtt ttcttctttt ttaccaaaga
    18481 acggtgcaac cttatccaca aaccaaatcg caaattgttg ttggtgacca atcgcaaacc
    18541 caccgccacc tgtaaggcgt tgagttgctt caacggtcat attagaactg atagcccagt
    18601 aaagcccaca gatgatacca actgcccacg ccccaaaagc attttggaat tgagggatta
    18661 aaagaaggac aaagacagag atagtcgctg cctgttggac catgatgtgg cctgtgatga
    18721 aaagagtacg aactttagtg accttacgaa gtgcaaccaa aagaatgttg accccaaatc
    18781 caatcaagag agctgtcgta gcaacactga tgaagcccat ctcttctaac ttagtattag
    18841 cagcagccaa accaaaataa ggatcaataa cggcggcttt caattcaaat ttcttggcaa
    18901 gagcaaccaa gatcggacga aaagtcgtta caagaccgcc tgctccaacg ttcaaaatca
    18961 agtaaccaac agtagccttt acaaaaccag caaagacctc ataaatgggt tttttaagga
    19021 gtaaataccc gattaaaacc aaaagaccta caaagaacgc tgggttttgc aaaatatttt
    19081 gcgagaacca gtttagggga gctaggagca tttccataag aactacctct tttcaaagtt
    19141 aataaagata aagtgtttat atgttcatta taggtaatga aagcgtttta aaaaagacca
    19201 actatcacac aaagaagtgt tcaatattag acttaagtta acttatttta aaatttaact
    19261 acacttaata ataaggagga aataccccta aacaacgatt aaaaaccaca aaaacgaagg
    19321 ttcttgtggt ttttctagat gagttgcttt ttgaatagat cagctctttg gcattaactg
    19381 tgacgtgacg caaataacca ctgaagggct gtgtcaagtc aaactatttg ataacaatat
    19441 taacgagttt gtttggtact gcgatgactt tgatgatgtc tttgcctgcg atttcggctt
    19501 ggactttttc gttagcaagg gcaacttctt gcaattcttc gcgtgaaagg tctttggcaa
    19561 ctacaagttt agctttgacc ttacctttga tttggacaac gatttccaca tcgttttcaa
    19621 ccaatttgct ttcatcataa cttggccatg gcacgtaaga gattgactca cctgaggctg
    19681 tcaaggcttg ccagagttcc tcacctaagt gcggtgcaaa tggtgcaatc aattgaacaa
    19741 agcctttggc atagtcgcta aagagtttgt cttccttgtt ggctgcattg acaaagacca
    19801 tgagttgagc gatagctgtg ttgaatttca tttggtcaac ttgctcagta acggccttaa
    19861 cagtttcgtt gtaaaccttg tcaagggctc cgctattttc ctcagtaatc tctttagacg
    19921 tgatcaaacg gtaaacacgg tccaagaact tacgtgagcc ttcaagacct tcttcagacc
    19981 aagcgatgga agcgtcaagt ggtcccatga acatctcata aacacgaagg gtgtcagcac
    20041 cgtactgctc aacaacatca tctgggttga caacgttctt aagtgattta gacatcttag
    20101 ctggtgcttg ttcaagctct tcaccagttt ccacatggaa gaagctgcca tcacgttttt
    20161 caaccttgtc agtcgctaca agcgctccac ggctgtcgcg gtagcttgtt cccaaaatca
    20221 tcccttggtt aaaaagtttt tggaatggct ctttggttgg tacgacaccg agatcgtaaa
    20281 gaaccttgtg ccagaagcga gcgtagagaa ggtgaagcac ggcatgctct gcaccaccga
    20341 catagatatc aactggcaac cattgtttga ggagttcctc atcagctagt ttttcggtgt
    20401 tgtgtggatc gatgtaacgg agatagtacc agcttgaacc tgcccattgt ggcatggtgt
    20461 tagtctcacg acgaccttta acaccatctt cacgagtcac ttccaaccag tctgtcacgt
    20521 tagcaagtgg gctctcacca gtacctgaag gacgaatatc cttagttact ggaagaacca
    20581 gtggcaactc actctcagga accgctgttg aggtcccatc ttcccagtgg atgattggga
    20641 ttggctcacc ccagtaacgt tgacggctaa agagccagtc acggagacgg taagtgactt
    20701 tttcattacc gacgccttct gcttcaagcc attcgaccat tttggcaata gctgacgctt
    20761 tgtcaagatg gtctaggaag cctgagttga tgtgaagacc gtcttctgta aaggcagcct
    20821 cttcaacatt accaccttca agaactggaa tgatatctag gttaaattgt tttgcgaatt
    20881 cccagtcacg ttcatcgtgg gctggaacag ccatgatagc accagtaccg tagctggcaa
    20941 ggacatagtc cgcaatccaa actggcattt cgttaccatt aactgggtta atcgcatagc
    21001 tacccgtcca gacaccagtt ttttctttgg caaggtctgt acgagcaagg tctgatttga
    21061 gacttgcttg acgtttgtag tcagccacgg cttctgcttg gtcagctgtt gtaatagcat
    21121 caaccaaggc atgctcagga gcaagaacgg cgtaagttgc accaaaaagg gtgtcaggac
    21181 gtgttgtaaa gacagtgaag tccttatctg tatccttaac cttgaaggtt acattagcac
    21241 cagttgattt accaatccag ttgcgttgca tgtccttgat tgattctggc cagtcaactt
    21301 cttctaagtc ttctagcaag cgctctgcat aggcagtgat tttgagcatc cattggcgca
    21361 tgggttttct gacaactggg tagccgccac gttctgatgt cccatcaggg agaacctctt
    21421 cgttggcgat agctgttccc aattcttcaa cccagttaac gggaacttca gcttcgtagg
    21481 ctaggccttt ttcataaagc ttagtaaaga tccactgtgt ccacttgtag tagttagggt
    21541 cagttgtgtt gacttcgcga tcccagtcgt atgagaatcc gagagcgtta atctggcgct
    21601 taaagttagc aatgttttca gctgtaaact cagctgggtc attaccagta tccatagcgt
    21661 actgctcagc aggaagtcca aaagcatccc aacccattgg gtggaggaca ttgtgacctt
    21721 gggcacgttt gaaacggctg agaatatcag ttgctgtata gccttctggg tgaccaacgt
    21781 ggagaccagc tcctgatggg tatgggaaca tatctagggc ataaaactta ggttttgatg
    21841 cgtctgttcc tgttttaaag gtatgattat ctgcccaaaa ggcttgccat ttgggttcaa
    21901 tagcggtgtg atcgtaaaat gtcatgtgaa atctcccatg taaaaaatct agtcttctta
    21961 ttatagcatg agatgaagct tttaggaatg gtcaagacat gactagcacc ttggcttttg
    22021 agacttcaga cgattagcta tttttaaacc ctgctttagc atcaaccatc ctcacaaaaa
    22081 gctaataaaa aagccagact tgctctgtaa acagagccaa tctggacaaa tagacttaaa
    22141 aagcaagtgc ttgctcaaaa tcggccaata agtcttcgat attttcaaca ccacaagaca
    22201 agcgcagcaa accatcaaca accccttgtt ccaaccgcgc ttgtgatgat aaacaagcat
    22261 gagacatcgt tgctggatgt gacaaaatgg actctactcc acctagacta acggctaaaa
    22321 tcggcaactg aatacgatgg gtaaaggtca tcagctcctc cttagaagcc aactcaaagg
    22381 ataaaacagc accaccattc ttagcttgtt gcaggtgggt atcgtgatta ggatgactgt
    22441 ctaagccagg gtaatgtacc ttaagaactt tggggtgctt cgctaaatag ttagcgagct
    22501 gctgagcatt ttttaccgct tgttccatac ggatccccat agttttcatg ccacgaagaa
    22561 tcagccaagc gtcttcgacc ccaacaattg ctccaaagtt tttttgaaaa agcttgagct
    22621 ggttgtagat agcctcatta ttggtagcag ctaaaccagc taccacatca ctatgaccat
    22681 tgataaattt tgtcacactt tctaccacaa tatccacacc catagctaag ggttcttgat
    22741 acaaacttgt cataaaggta ttatcagcca cagttagcac accataagct ttggcgagtt
    22801 gcacaagctc tgaaatatca caaattttca agagcggatt tgacggtgtc tctagataaa
    22861 tcatacgcgt attaggcctg atacttgctt ttactgttgc taaatcagcc atatctacaa
    22921 agcttgtttc aatctgataa ttgggtaaaa tagctgtcgc aaattggcaa gtccctccat
    22981 agacctccaa tgggaaaatc acatgatctc cagcttttaa gagcatcaaa acagttgaaa
    23041 tagcagccat accagatgca taagccaccg cataagtcgc tttttctaaa caggctaacc
    23101 cgtcttctaa agcttcagtc gttgggtttg aaaagcgtgt gtacaaatgt ttttgactag
    23161 gacaatacag ttctgaattg tggaacgtcg aagtctgata gatgggaact gaagctgctc
    23221 ctgtaaactc gtcaataacc gtatagccat gtaataaggt cgtattttct ctcatttcgt
    23281 gcacctccgc aatagcgtta actccaatga actctctttt agacaatggt cccaccccaa
    23341 tggttgcaca aaccaaaaag ctcttcaaaa aagcgtgatc tgctaaccta ataagaaaag
    23401 tagtgggcac cttttaaatg gataatgaat ttttccaaca atatcatttt tagatcgatt
    23461 acgtttatta tacaaccttt agctaagttt gcaagccctt ttatcatctt tgaccaagct
    23521 gccaacacct ctctagcaag ttaaagtata gctacctttg gccccatcgt ctcaaaagct
    23581 tagcttatca cactgacagt cctactttgc atcgtcacct actcaatagg gcaatattgc
    23641 catcaaaatt tcattaaaag gtcacaattt atttcattta atgagaaaca gaacttgttt
    23701 tcaccatgcg ccttatagta cactgctaaa gtactattta tttttaggag agaaatatag
    23761 tgaaaaacaa attattttta gttgcccttg cgaccgtaac tgtcctaggg ccgtctttag
    23821 caacccctca tcaccagacc gtgcatgcgg ctgagcaggc actggcagga acatgtacat
    23881 actctaatga gaatccttat gtaggtgttt gcttcgagag aaaagatggc atgccaatag
    23941 aagaaaacat tatcacaaaa aataacgatt tttcccttct taactggttt tgggagatag
    24001 ttaccaattg gtggtcaaaa ataacaggtt atttttcgta atactagtca cagcatagtc
    24061 taccgctaac tattaaaata aaaaaccatc tcgtggcggt tttctaatcc taggtcgcca
    24121 catttcttat tcagatgaat aataataaaa acattaaaat aatacttgat ttattaacaa
    24181 aaagttagta taatatagtc atactacaaa ttaaaggagt tatctatgaa aaagaaatta
    24241 tctttattta tgatcgcaac tgctgctgtt gcaggcctta gccttgcaac actaggagag
    24301 caccaaactg tttcagcaaa tgatgctaac tatagtaacg gtttctacgg acacaatggt
    24361 cctgagagct ggttacttta cacagtatgg taaacctcat accgtaacat agaagctatc
    24421 ttcttcgtaa gactacggtt atgaaaagcc tagggctgca taagcagcct taggcttttt
    24481 gattggctaa aacgtttttg ccaatgaccc ctcaaggtaa aacagaaaag gcacaatgcc
    24541 tataaaaaga gagaagccca aaacacaagt cgtaacagtt gctagctaac cagcaccaaa
    24601 aaagctgtaa cacctcaaat agaatgataa tcagattaat attattaata attaatgtga
    24661 tttatataag ataaagaaat gctttagtct tggctttctt tgcctttttc ggtcaaacat
    24721 attacaaaaa ga
//
LOCUS       DAUCWO010000023        24513 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUCWO010000023 DAUCWO010000000
VERSION     DAUCWO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24513
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.023"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..326
                     /gene="amaP"
                     /locus_tag="VGY20_000853"
     CDS             <1..326
                     /gene="amaP"
                     /locus_tag="VGY20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ4273720.1"
                     /translation="LRHKNKKGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRK
                     FKIIVKGRLASRVAVADQISGVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHIT
                     RNRVE"
     gene            337..525
                     /locus_tag="VGY20_000854"
     CDS             337..525
                     /locus_tag="VGY20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ4273721.1"
                     /translation="MEFYEKFKYPIIGGLVGLILAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            538..1077
                     /locus_tag="VGY20_000855"
     CDS             538..1077
                     /locus_tag="VGY20_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4273722.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1118..1318
                     /locus_tag="VGY20_000856"
     CDS             1118..1318
                     /locus_tag="VGY20_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ4273723.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1329..1817
                     /locus_tag="VGY20_000857"
     CDS             1329..1817
                     /locus_tag="VGY20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4273724.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            1980..2621
                     /locus_tag="VGY20_000858"
     CDS             1980..2621
                     /locus_tag="VGY20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4273725.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(2764..3306)
                     /locus_tag="VGY20_000859"
     CDS             complement(2764..3306)
                     /locus_tag="VGY20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4273726.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            3424..4125
                     /locus_tag="VGY20_000860"
     CDS             3424..4125
                     /locus_tag="VGY20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273727.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4140..6776
                     /locus_tag="VGY20_000861"
     CDS             4140..6776
                     /locus_tag="VGY20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ4273728.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELVVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSTTSLSPYFNLLDC
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFRNHFKGLEAASAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            6868..7464
                     /locus_tag="VGY20_000862"
     CDS             6868..7464
                     /locus_tag="VGY20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ4273729.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDLPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            7475..8431
                     /locus_tag="VGY20_000863"
     CDS             7475..8431
                     /locus_tag="VGY20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ4273730.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGSQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            8528..9868
                     /locus_tag="VGY20_000864"
     CDS             8528..9868
                     /locus_tag="VGY20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ4273731.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKSQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFAKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKGCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAAMNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            9984..10868
                     /gene="truB"
                     /locus_tag="VGY20_000865"
     CDS             9984..10868
                     /gene="truB"
                     /locus_tag="VGY20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ4273732.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            10897..11829
                     /locus_tag="VGY20_000866"
     CDS             10897..11829
                     /locus_tag="VGY20_000866"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ4273733.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFLRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            11872..12276
                     /locus_tag="VGY20_000867"
     CDS             11872..12276
                     /locus_tag="VGY20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ4273734.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12278..12556
                     /locus_tag="VGY20_000868"
     CDS             12278..12556
                     /locus_tag="VGY20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ4273735.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLNS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            12546..13334
                     /locus_tag="VGY20_000869"
     CDS             12546..13334
                     /locus_tag="VGY20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ4273736.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            13337..14647
                     /locus_tag="VGY20_000870"
     CDS             13337..14647
                     /locus_tag="VGY20_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ4273737.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHINLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            14786..15652
                     /locus_tag="VGY20_000871"
     CDS             14786..15652
                     /locus_tag="VGY20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ4273738.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            15663..16598
                     /gene="pstC"
                     /locus_tag="VGY20_000872"
     CDS             15663..16598
                     /gene="pstC"
                     /locus_tag="VGY20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ4273739.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFIDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTVLSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            16588..17475
                     /gene="pstA"
                     /locus_tag="VGY20_000873"
     CDS             16588..17475
                     /gene="pstA"
                     /locus_tag="VGY20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ4273740.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            17491..18294
                     /gene="pstB"
                     /locus_tag="VGY20_000874"
     CDS             17491..18294
                     /gene="pstB"
                     /locus_tag="VGY20_000874"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273741.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            18307..19065
                     /gene="pstB"
                     /locus_tag="VGY20_000875"
     CDS             18307..19065
                     /gene="pstB"
                     /locus_tag="VGY20_000875"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273742.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19133..19786
                     /gene="phoU"
                     /locus_tag="VGY20_000876"
     CDS             19133..19786
                     /gene="phoU"
                     /locus_tag="VGY20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ4273743.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            19991..22528
                     /locus_tag="VGY20_000877"
     CDS             19991..22528
                     /locus_tag="VGY20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ4273744.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQSTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
     gene            22875..23549
                     /locus_tag="VGY20_000878"
     CDS             22875..23549
                     /locus_tag="VGY20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4273745.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
     gene            23542..>24513
                     /locus_tag="VGY20_000879"
     CDS             23542..>24513
                     /locus_tag="VGY20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4273746.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNR"
BASE COUNT         7820 a         4052 c         5046 g         7595 t
ORIGIN      
        1 agttacgcca taaaaataaa aaaggaacac ttttattaaa aaaatcggct attgaaagtt
       61 atgttgcgac cgctattcaa actgcaggtt tgatgccaaa cccaacagtc acagctaaat
      121 tatataagcg gaagtttaag attatcgtta aaggacgttt agcttctcgt gtagcagtgg
      181 cagatcaaat tagtggcgtg aaagaaggga tcgaaaaagg tttaactgag ttcttcggaa
      241 ttaattatcc tgttaatttc aaagtttatg ttaaagatat tgctgattct gatcgtaaac
      301 atattaccag aaatcgtgta gaataggaga tctatcatgg aattttatga aaaattcaag
      361 taccctatta ttggtggctt ggtggggcta attcttgcca tattgctcat ggcttttggt
      421 ttattcaaaa cgctactagc aattattttc attattttag ggatatatgg cggcctgtat
      481 gccaaaaaaa caggtattat tgatcaattt cttaatcgca aataaaggag acttattatg
      541 actgaaactt atattaaaaa cacatctaaa gacttaactt cagcaattcg tggtcaatta
      601 acatatgatg ataaagtaat tgaaaaaatt gttggtttag cacttgaaaa tgttgatggg
      661 ttactgggtg taaatggtgg tttttttgct aacctaaaag ataaattagt aaacaccgaa
      721 tctgtacgtg atggcgtgaa cgtggaagtt ggaaaaaaac aagttgccgt tgatcttgat
      781 attgtcgctg aatatcaaaa gcatgtgccg actatttatg actctattaa atcaatcgtt
      841 gaagaagaag tgaagagaat gaccgatctg gatgtgattg aagttaatgt taaagtagtc
      901 gacattaaaa caaaagaaca atttgaagct gaaaaagtaa gtttacaaga caaagtttca
      961 gacatggcac gttcaacatc tgaatttacg agtcatcaag ttgagaatgt taaagcttca
     1021 gtggataacg gtgttgaaaa acttcaagat caaaaagctg agccacgtgt aaaataatta
     1081 tatgtcgtca attaggtaag ctaaaggagg gtttattgtg tcagatgaaa aatataatgc
     1141 gaaattagat caagctggtg gcaagttaaa agaaggattt ggtaagattt ctggagataa
     1201 atctttagaa actgaaggta aagtagataa agttactgga aaagttaaag aggttattgc
     1261 agacgccaaa gatactgtaa aaggattagc taaaggctta gataataaag ataaataaag
     1321 gggacatgat ggaaaaaact caaattaaaa gcactcttac ttatgacgat aaggtcatcg
     1381 aaaaaattgt cggtcatgct ttggaaaacg ttggcggatt actagctgta acaggtggat
     1441 ttttctcaaa tatcaaaaat aatttggtga attctgagtc tgtaactgat ggtgtttcag
     1501 ttgaagttgg tagcaaggaa gtagcagttg atttagcaat cattgtagaa tacggtaaag
     1561 atattcctgc tattgcagaa tctattaaag ctattgtttc tcaaaacgtt gacagtatga
     1621 cacatttgaa agttgttgaa gtcaatgtga acgttgttga tattcgtact aaggaagaac
     1681 atgaagctgc tagcgttact gttcaagatc gtgtcactag tgcagcttca agtacttcac
     1741 aatttgtatc agaacaaaca gaaaaattaa aagatactat ctctgatact gtaaatagtg
     1801 atgaggcagc taaataagca atcgtctagg aagtattatt gactagtggt gataagtttg
     1861 agctggtatt taccagctct ttttgtcacg gttaaaaaga tacggactat tgtcatctct
     1921 ctaaaaatat gatttaatta gatagaaatt tttcaaattg ttagaaagga gtaaattaag
     1981 tgataagtcc taaaaaagag ataacaagtt caaaatatca aaaaattgct atttcagtag
     2041 ctcagcgtat cgcaaatggc gaatatgaag tgggcgaaaa gttaaagtca agaactacta
     2101 tcgcctcgac atttaatgta tcaccagaaa cggctcgtaa aggcctgaat attttagctg
     2161 acttgaaaat tttgacttta aaacatggta gtggagctat tgtgctgtca aaagaaagag
     2221 ctattgagtt tataaatcaa tacgaatcaa cccattccat cgctgtttta aaagaaaaaa
     2281 tacgtgagac tattaatgat cagggaaaag caatggaaaa aatggctgtt ttggtcaatg
     2341 attttttaat gcaaagtcaa tctgttagta agcaataccc attagcacct tatgagatta
     2401 tttgtagtca agattcggaa cattttggca aatctattgg tgtgttaaat atctggcacc
     2461 agacaggagc aactatcgtc gctattgaac atgctggtca gtttatcgtt tcaccaggtc
     2521 cttattctgt tattgaaaaa ggcgatcata tttatttcgt tggtgatgag tcagtcattt
     2581 cacgaatgaa gacctttttt aatttacgaa aaggattata agtttgagga cccacctcaa
     2641 actttttttg ttgctgttta gcattattaa aagaggcaga tcatcatggt ttgtctttcc
     2701 aaaatatctg tttagacagt tgttttggat tgggagcatg atttcagact cttttgatgt
     2761 tttttaattg tctagagtaa ctgctttgag aataatatcg gcaacctctt cagtggtttc
     2821 ttgatagcca ttagcaatcc aatagctgat aatgctctca atactggcaa ggtaaacttc
     2881 taaagcatag ggataaggaa tatggtattg attggtcaca atgtcttgga aaccagtaat
     2941 agtagtcaaa aattggtaac agaaatcctt gatgagttgc ggaaatttga ggtaagaaat
     3001 agtagcgagt gcggttataa attctctatg ttctataagg taggacaagg tttgattgag
     3061 gacttgtcga gtgtctgtgt agatttcagc ctgatttaac aaacggtaca aatcatccag
     3121 agtgtcattt ttgaagtgat tcatcatgtc aaatttatca gtgtagtgga ggtagaaggt
     3181 tcctctattg ataccagcct ttttagtaag gtcagagaca gtaagagttt caaaagattg
     3241 ttcagttagt aaggtggtta aggcggtctt tacataagct ttggtttcag tatgtctttt
     3301 ggtcattctc aggtgctcct taaatcaaca gttgcgtcag atttgttctt gttaaatact
     3361 tagggccatt atataataag gctataatta aatcaacact atgttcaaat aatggagggg
     3421 cagatggctt ttattgaatt aaaacaggtt tcaaaatctt atcaggtagg agagacgact
     3481 gtttttgcca accacgaagt gtcttttgag attaataaag gagaactagt ggttattttg
     3541 ggagcatccg gagctggtaa atcgacagtc ctcaatattt tggggggcat ggacacagta
     3601 gatgcaggtc aagtaattat tgatggcaaa gacattgctc attacacatc taaggcctta
     3661 actcagtatc gtcggaatgc tattggtttc gtttttcaat tttataattt ggttcctaat
     3721 ttgacagcta aagaaaatgt tgaattagca gttgaaattg tagcagatgc tttagatcct
     3781 gtgaccattt taaaggaagt aggactcagt catcgtttgg atcattttcc tgctcagctc
     3841 tcaggtggtg aacagcaacg ggtttcgata gcacgtgcct tagctaaaaa ccctaaattg
     3901 cttctttgtg atgaacctac aggtgccctt gactaccaaa caggaaagca aattctaacc
     3961 ctcttacagg atatggcacg aactaagggg accacggtag ttattgtaac ccacaatgct
     4021 gctattgctc cgatagctga tcgtgtcatt tttatgcatg atgcacaggt taccaagaca
     4081 gtcattaata aggaacctgc aagcattgag acaattgatt attaagagga aggtgtcgta
     4141 tgataaagaa aaccttatgg aaggatattt tgagagctat caagaatagt aaagggcgat
     4201 tcatttcctt atttttccta atggccttag gctcctttgc tttagttgga cttaaagtca
     4261 ccggtccaga tatggaacga acagccagcc gttacctcga aagacaccag gtgatggacc
     4321 taacggtact ggcttctcat caattttccc aagccgataa acaagagtta gatacgttaa
     4381 aaggggctca tttagaatat ggtcatttac ttgatgtcag tctaactagc aaccagaaat
     4441 ctcttaggct ctatagcgtg ccaaagaaag tgtctaagcc agtcttggtt aaagggagtt
     4501 ggccaaagcg agagacagat ttggttttat cctcctcact tgctaaaaac tatcagattg
     4561 gagatgaact agtagtcacc tcacctatgg aaggtttgct gacgacaacc cattttcaag
     4621 tggttggttt tgcgaattct tcagaagttt ggtccaagtc caatttagga agttcctcaa
     4681 ccggagatgg aagtctctac gcttatgctt ttgttaatcc aaacgttttt aaaagtgcct
     4741 ttaacctgct gcgcatccgt tttagtaatc ttcgtctgac aaacgctttt tccaaagact
     4801 atcaaaaaag ggtaacccaa aaccaagctc atttggataa tctacttaaa gataatggcc
     4861 agaagcgtta tgatgacctc caaaatcagt atgaccttgc cttaaaaaat ggcagagcag
     4921 cacttgcaaa ggaaacagtg aaactagctg cgagtgagga gaacttaact tttttagaag
     4981 gctctgcttt acaagaagct aagcatcaga ttgaacaagg caaacaagca ttagccaagg
     5041 aggaaaagca gttagagcag gtgcaggcta caaaagataa gctagaaaaa cccagttacc
     5101 tgacttataa tcgctcgacc ctaccaggag gagaaggata tcatacttat gcaacttcaa
     5161 cgacctccat ttcaaatgtt ggaaatattt ttcctgttgt tctttatctc gtagctgctc
     5221 tagtagcctt taccaccatg acacgttatg ttgatgaaga aagaacaagc tctggtctat
     5281 taaaagccat cggttattct aacaaggata tcagtttaaa gtttcttatc tatgggcttt
     5341 tagccagttt tttagggaca actttaggta ttattggggg aacttacctc ttatctgcct
     5401 tgatttcaga gatcttaaca ggagctttga ctattggaaa gactcacctt tatagttatt
     5461 ggttttataa tggcatagct tacttgctgg ctatgttatc tgctgtttta ccagcctact
     5521 taattgtcaa aaaggaatta ttcctcaatg cagctcagtt attgctgccc aaacctccta
     5581 gtaagggggc aaaaatctgg ttggaacacc tgacttttgt ctggaaagct ctgtccttta
     5641 ctcacaaggt gaccatacgt aatatttttc gctataaaca aagaatgctg atgaccattg
     5701 taggcgttgc aggctctgta gcccttttat ttgcaggctt agggattcag tcgtcattag
     5761 ccaaagtagt tgagcatcaa tttggtgatt taacgactta tgatattttg gctgtcggtt
     5821 cggccaaagc gacagcgaca gagcaaactg acttagctag ctatcttaaa caagaaccta
     5881 ttacagggta ccaaaaggta tcttatgcca gcttaaccct tcctgtaaag ggattacctg
     5941 ataagcaaag tatttccatt ttatcaagtt caactacctc tcttagcccc tattttaatc
     6001 tgctggattg tcaggagcaa aagaaggttc ccattccaac ctctggtgtt ttgatttctg
     6061 agaaattagc ctcttattac aaggtaaaac caggtgatca gttggtattg actgatcgga
     6121 aaggacagtc ttataaagtg acgattaaac aggttattga catgacagtt ggccattacc
     6181 tgataatgtc taatacctat tttaggaatc attttaaagg attggaggct gcttctgcct
     6241 atctgattaa ggtaaaagac aaggatagca agcacataaa ggagacagcc agtgacttgt
     6301 taaccttaaa agcgattaga gcagtttcac aaaacgtcaa tcatattaaa tctgttcagc
     6361 tagtagtcac ctctcttaat caggtcatga ccctccttgt cttcttgtct attttattag
     6421 caatcgttat cctttataac ttaacgacta ttaatattgc tgagcgtatt cgagaattat
     6481 ccactattaa agttctggga ttttacgatc aggaggtcac cttatatatt tatcgagaaa
     6541 ctatttcgct atccctagta ggcattcttt taggtatcta tttaggaaaa ggcctgcata
     6601 cttatatcat gacaatgatt tcaactgggg atattcaatt tggtgtaaag gttgatgctt
     6661 atgtttatct agtgccaatt ctagtaatcc ttagcttgtt agcggtatta ggtatctggg
     6721 ttaatcgcca tttaaaaaag gttgatatgt tagaagcttt gaaatccata gattgattgg
     6781 tgaaaagaca gagatagaca gcacttgaaa agttaatcat attatgatag tatctaaata
     6841 ctataattta atagaaagat actatgtatg gaaaatagca ttgaggccta tctagcagat
     6901 agcagagaaa aggtaacatt aggtacagtt gttgattgtt ttaaaggaaa agcagtctca
     6961 agtaaggttg tgccaggtga tgtgggattg attaatttat ctgacatggg caccttagga
     7021 attcaatatc accaattaag aactttccag atggatcgta ggcagctctt gcgttacctt
     7081 ttggaagacg gagatgtctt aatcgcctct aagggaaccc taaaaaaagt ctgtgttttt
     7141 cataagcaaa atcgagatgt ggtggcatca tcaaatatca ctgtcttacg ccctcaaaaa
     7201 ctgttaaggg gctactatat caagttcttt ttggacttac ctattggtca agcgctcttg
     7261 gacgcagcgg accatggcaa agatgtgatc aatctctcaa cgaaagaatt gttggatatt
     7321 ccaattcccg ttattccttt agtgaaacag gattatttga ttaatcacta cttgagagga
     7381 ttaacagatt atcatcgtaa actcaatcga gcagaacagg agtgggaata tattcagaat
     7441 gaaattcaaa aaggattagg ttaggagtct tgtcatgggt tcagttgaac gtacattaaa
     7501 aatgaccttg gctaccattg ttgccatttt aattgcttac caacttcatc tggattatgc
     7561 aatgtcagcg gggatcattg ccctgttaag tgttttagac acccgtaagt ccagtttggt
     7621 ggtcgctagg aatcgcttgc tatctttttt cctggctttt gggattgcga tgatgtgctt
     7681 tagcttgttt ggttttacga cagtaggttt tatgtgttat ttgttaatta ttattccttt
     7741 gctctatcac tttcagatag aggcaggact ggttcctata acagtacttg tgacacattt
     7801 gattgctaaa aagagtattg cattacctat tttatcgaat gagtttatgc tcttttttgt
     7861 agggactagt gtcgctttac tatttaatgc ttatatgggt tcacaagatc agcaaattcg
     7921 gtactatcac caaaaagttg aatcggatct gaaaggtatc ttataccgtt ttgaaagttt
     7981 tctactggaa ggaaaaggtc aaaatgaagg gctcttgatc aaaaaccttg ataaaatatt
     8041 agatgaagct ctaaaactag tttatcgaga aaggcataat caactatttc agcagaccaa
     8101 ttatcaagtt cattattttg agatgcgacg acaacaaaat agactattgg gacaaatggc
     8161 tatcaacgta aacacgttaa tgagacaaag taaggaaagt attcttttgt cacatctttt
     8221 tcacgaaact gcttgtcagc taagcgaaca aaatccggct ttaaccttga ttgatgacat
     8281 cgaacaattg cttgaaacct ttcgtcatgg tgatcttcct caaactcggg aggaatttga
     8341 gcgacgtgcg gttttatttc agctcttaca agacttagag cgctttatct tattaaaggt
     8401 agagttttat caggattatc aaaatgacta aatctctatt gcacacttct tatctatgac
     8461 agcttgggac ttaacttcct gagctttttt gctataataa aaagaagaat ctcagaatgg
     8521 agacaagatg aacgaaataa aatgccctca ttgtcatacc ctatttacta tcaatgagtc
     8581 agagtatagt caattattgg aacaagtcag agggcaagcc tttgatgaag aattgcaaaa
     8641 acggctaatc aatgagatag ccttgctaga agaaaaagcg aagcatcaat tgcatgaggt
     8701 tgtcgcaaaa aaagaaacag ctattaccag cttgacaaac caattagagc aaattgaaaa
     8761 agaacaagcc tacctcagac aagaagaact ggctaaaaaa gaccagctta ttgcttcttt
     8821 agaagcgaaa cttgataaat tagcctcaca aaatgctttg gaactggcca atcaattagc
     8881 tgagaaggac aaagaagtgg ttagtttgac caatcagctg gacaaattag cacttgaaaa
     8941 agatgccacg ttccagtcaa agcttgctac aatcgaaaaa gaacgcgatg gaatcaaaaa
     9001 ccaattggcg cttcaagcta aagaaagtga attgtcgttg gcttcggtgc gcagcgacta
     9061 cgaagcacag ttaaaagcgg ctaacgagca agttgaattt tataaaaatt tcaaatcgca
     9121 gcaatctacc aaagcaatcg gagagagtct agaactctat gctgaaacag agttcaataa
     9181 agtaagaagt tatgcttttc ccaatgctag ctttgctaaa gataatcaat tgtctagtcg
     9241 tgggtcaaaa ggggattata tttaccgaga ggttgatgcc aatggtgtag aaatcctctc
     9301 tatcatgttt gaaatgaaaa acgaggcaga caccaccaaa acaaaacata aaaatagtga
     9361 ttttttcaag gagttagata aagaccgtcg cgaaaaaggt tgtgagtatg ctgttttggt
     9421 aagcatgtta gaagctgata atgattacta caatacaggg attgttgatg tcagtcatga
     9481 atatcaaaag atgtatgtgg ttcgtcctca actctttatc cagttgatag ggattctgcg
     9541 aaatgcggcc atgaatagtt tacattataa gcaagagttg gccttgatta aggaacaaaa
     9601 tattgacatc actcacttcg aagaggattt ggaccaattt aagaatgcct ttgctaagaa
     9661 ctatcagtct gccagcaaca actttaaaaa ggctattgac gagattgata aatcgattaa
     9721 acgcatggag gaagtgaaac gctttttaac cactagtgaa aatcagttac gcttagctaa
     9781 taataaatta gaggatgttt cagttaaaaa actgacccgc caaaacccaa ccatgcgaga
     9841 gaaatttgaa gccctcaagg accagtaagc ctgaaagtga gtctttccct gctaaaattg
     9901 gttaagcgtt tattttggga aaactgatga taaagcggta gaatagaatc agtaataatt
     9961 actaattaga gagaaaaata gacatgataa atggaatcat taatttaaaa aaagaagccg
    10021 gcatgacctc tcatgacgca gtttttaaat tacggaagtt gttacaagaa aaaaagattg
    10081 gacatggggg gaccttagat cctgatgtgg tcggtgttct accaattgca gttggtaagg
    10141 ctactcgtgt gatcgaatac atgacagaag ctggtaaagt ttatgaagga caagtgactc
    10201 tcggttactc aacaacgact gaggatgcta gtggagaggt agttgcgagg tcctctttgc
    10261 cagctgtgct aacagaagaa ctcgttgatc aaacgatgac gactttcctt gggaaaatca
    10321 cacagacccc acctatgtat tcagccgtaa aggttaatgg ccgtaaatta tacgagtatg
    10381 cgcgtgctgg tgaaagtgtg gaacgtccta gaagagaagt gactatttct ctgtttgaac
    10441 gcaccagccc tttgaatttt actgaagacg gtctttgccg attctctttt aaagttgctt
    10501 gtagcaaagg gacttatgtg cggaccttag cagttgattt aggtcgagct ctaggggtag
    10561 agagtcatat gtccttcttg cagcgatcag cttcagcagg attaaccctc gagactgcct
    10621 atactttggg agaaattgct gatatggttt ctaaacaaga gatgtctttt ttgttaccga
    10681 tcgagtatgg cgttgctgat cttcctaaaa tggttattga tgatactgaa ttgactgaaa
    10741 tttcatttgg tcgccgtctt agtctcccta gtcaagagcc actgttagct gcttttcatg
    10801 gcgaaaaagt aattgctatt ttagaaaaaa gagatcaaga atacaaaccc aaaaaagttc
    10861 ttatttaaat agtttgagta agtagaatgg ataattatgg aaattgaata tatcaaagat
    10921 tatcgtgata ttaaccaaga agatgataca gtcttggtat tgggatattt tgatggatta
    10981 caccgtggtc ataaagcctt atttgacaaa gcaagagagg ttgcaaataa agaagggtta
    11041 aaagtagtcg tttttacgtt tacagagtct ccgaaattgg cttttttgcg tttttctcct
    11101 gagttattgc ttcatattac ttatccaaag aagcgttatg aaaaatttgc cgattacggt
    11161 gtaaataaat tatatttggt tgattttact tctaaattct ctaaggtctc atctgatcat
    11221 tttataactc attatattaa aaacctgaaa gcaaagcata ttgttgtcgg ttttgactat
    11281 aagtttgggc ataattgtac agatagtgat tatttaacca gaaattttga aggacaagtt
    11341 tataccattg aggaaataaa agaagatcat cgaaaaattt ctgctacatg gataaggaag
    11401 cttattcaag aaggtaatgt agttaaagca aaccacttgt taggctatga cttatcgaca
    11461 agggggagag tcgttcacgg ggatgcgcgt ggccgtacta ttggtttccc aacagccaat
    11521 ctagctccaa ttgacaacac atacttacca gctgatggag tctatgttac aaatgttatt
    11581 gttgctaata aaatctatcg ctctatgacg agtttaggta aaaatgtgac atttgggggg
    11641 aaagagttgc gtctagaagt taacattttt gactttgacg aagagatcta tggagaaatt
    11701 attgagattg tttggttaga taagattcga gacatggaaa aattcgaggg aatcgaagat
    11761 ttgactgata gattggaata tgataaacga acagcattaa attggaaaaa agatagcaaa
    11821 ttatcttaaa tttttgtata ataaggtaag taaatttgat agaggaaaaa tatggttacc
    11881 ttatttttat cacctagctg taccagttgt cgtaaagcaa gggcatggtt agtaaaacac
    11941 gaagttgact ttcaagagca taatattatt acaagtccat taagccgtga tgaattaatg
    12001 tcaatcctct cttttacaga gaatggaacg gaggatatta tttcaacacg ttcaaaggtt
    12061 tttcaaaaac ttgatgttga tgtagaagag ctttcaattt cagatttaat tgatttaatt
    12121 gctaaaaatc caagtcttct tcgtcgccct attatcatgg atcaaaaacg catgcaaatt
    12181 ggttttaatg aagatgaaat tagagcgttt ttatcgagag attatcgcaa gcaagaacta
    12241 cgtcaagcaa cgataaaagc agaaattgag gggtgatatg tcaggtaatt attattatcc
    12301 acttgactta agttggagca ctgaagaaat atcctcagtg cttcattttt tgaataaagt
    12361 ggagttagca tatgaaaaaa aagtagatgc aaaacaactc ctaaatagtt ataagactta
    12421 taaaaccatt gtcaaaagta aagctcagga aaagcaaatc gaccgtgatt ttcagaaagt
    12481 tagtggttat tcgacctatc aagttgttaa aaaagcgaag gcaattgaga aaggattctt
    12541 ttcccttgga aactaaatac gcatttgcaa ggcaaatcat taaagaagca gggcttttta
    12601 tcaagtcaaa aatgtcagag cagctagata ttcaggtgaa aacacaattt gatgatctgg
    12661 ttaccaatgt tgatcaagaa actcaacagt tactgatgga tcgtattcat cagacctatc
    12721 cttgtgatgc cattttagct gaagaaaatg acgttcgtca ccccattaat caaggaaatg
    12781 tttgggtcat cgatcccatt gatggaacgg ttaactttat tgtgcaggga tcacagtttg
    12841 cagtgatgat tgcctattat gaacaaggaa ttggccaatt tgggcttatt tatgatgtga
    12901 tggcagatca attactagct ggtggtggtg attttgaagt tactttaaac ggtgataagc
    12961 ttccagctta ccaagaaaaa cctttagagc gtagcttaat tggctgcaat gcaggcatgt
    13021 ttgccagaaa tgatcgcaac cttgctcatc taattgcaaa gactttaggt gtaagggttt
    13081 atggtggtgc aggtatctgc atggttaagg tcatgaagca ggaattactg gcctattttt
    13141 catttatcca accatgggat tacgcagcag caaaagtact aggcgataaa ctgggttatg
    13201 ttttactaac tattgacgga tatgagcctg attttcaaac acgtcaaaaa atcatgtttg
    13261 ttcctaaatg ccaactcact aggatagcgt cttttctaac aaaggactct gccgtcacac
    13321 ttgaagatca atagaaatga tgtcattacc aaaagaattt attaacactt atcaagcaat
    13381 actaggcaaa gaattagaag atttcttggc aagttttaac caagagccgg tcaatgcttt
    13441 tcggattaac cccttaaaga atcaactaaa aacctttgaa caccccattc caaatacctt
    13501 atggggatat tatggtaaac tatctggcaa gtctccagag catgtgtcgg ggttggttta
    13561 ttcccaagaa ccagcggctc aaatggtggc acaagtagcg gctcctcaaa aggggagtcg
    13621 tgttttagac ttggcagctg caccaggagg aaaatcaact catttattag cttatttaga
    13681 caatacaggt cttttagtca gcaatgaaat ttctaagaag cgcagcaagg ttttagttga
    13741 aaatatcgaa cgttttggcg ctagaaatgt tgtggtgact aatgaatccg cagatcgttt
    13801 agccaaggta ttttctcatt attttgatac tatcgtcttt gatggaccat gttctggtga
    13861 aggaatgttt cgcaaggacc ctgatgctat tcaatattgg catcacgggt atcccgcaga
    13921 atgtgccaag ttgcaaaaat ctattttaga agacgcctta gccatgttaa aaccaggagg
    13981 agaattgata tactccacct gtacttgggc tcctgaagaa aatgaagatg ttgtgcagtg
    14041 gctcttagag acttatacct ttttagaatt ggtcgatgtt ccaaaactaa atggaatggt
    14101 tagtggtatc ggacttccag agactgctag aatgtaccct catcgttatc aaggggaagg
    14161 gcaatttgtg gccaagctaa aagataagcg tcaagaagga caaagcacta aactaaaagc
    14221 acctaaatcc aacttgatca aagaccagtt acgtctttgg aaaatgtttg aaaaagacca
    14281 tataaacctt accctatcag gaacactaca aacctttggt gattacctat acctcttgcc
    14341 agatggtctt cctgctttag atggccttaa aattgctcgg aacggtttag aattaggcgt
    14401 cttcaagaag aaacgctttg aaccctctta cgccttggga ttagctttaa agcctgatga
    14461 ggtccgatca tctattgagc ttagagagga agactttaga ccttatgtgt caggtaacgt
    14521 cattactttg ccccaatctt atccaacagg ctggtaccaa ttactcatta atggcaatgg
    14581 tctaggattt gccaaggtcg ttgacaagac catcaaaaat aatttcccta aagggttaag
    14641 attttagcgt aaaaaataag attaaaaagt taaatattta aagaaaaaat gctttcaaag
    14701 cataggtaga tatgttacta taaagaatat gtggcactta gtttctacat atataccctt
    14761 tatttttacg aataggaaat aaataatgaa aatgaaaaaa aaattctttt tgttaagtct
    14821 tttggtccta tcaactttct ttttatccgc atgttctagc tggattgata aaggtgagtc
    14881 aataaccgct gtaggatcaa cagcactaca acccttagta gaagcagtag ctgatgaatt
    14941 tggaagcagt aatctaggca agactgtcaa tgttcaaggt ggtggttcag gtacagggtt
    15001 gtctcaagtt caatcaggag ctgtccaaat tggaaatagt gatgtctttg cggaagaaaa
    15061 agatggtatt gatgcttcta aattagttga tcatcaagta gctgttgcag gacttgcagt
    15121 tattgccaat cctaaagtca aggtttccaa tctcagtagt cagcagttgc aaaagatttt
    15181 ttcaggagaa tataccaatt ggaaacaagt tggaggagaa gatcttgcga tttcagtgat
    15241 caaccgagca gcaagttctg gctcacgagc aacctttgac agtgttatca tgaaaggggt
    15301 caacgctaaa caaagtcaag agcaagactc caatgggatg gttaaatcga ttgtttcaca
    15361 aacaccaggt gccatttctt acctttcctt tgcctacgtt gattcatctg ttaaatcttt
    15421 gcaattaaat gggtttaagg caaatgctaa gaatgtggct acaaatgatt ggccaatctg
    15481 gtcctacgaa cacatgtaca ccaaagataa accaacaggg ttgaccaagg aatttcttga
    15541 ttatatgttt tcagatgaag tacaacagaa cattgttaca catatgggat atatttcgat
    15601 aaatgatatg gaagtggtca aatctcatga tggaaaagta acaaaaaggt aatggaggaa
    15661 ttatggaaaa tcaggaattg gctaaaaaat tagcctcacc atcaaagaat tcaagacttg
    15721 aaacctttgg ccgcaccatt acgtttttgt gtctggcttt gattgttttt atcgttgcga
    15781 tgattctgat cttcgttgct caaaaagggt tgtcaacctt ctttattgac aaggttaatc
    15841 tttttgattt cttgtttgga aaggagtggc aaccaagtgt gaaaaatgcg gccggcattc
    15901 cttatcttgg tgctctgcca atgatcacag gatccttttt ggttaccgtt ttatctgcta
    15961 ttattgccac cccatttgcc attggcgcag ccgtttttat gactgaaatc tcacctaaat
    16021 atggcgctaa attattacag cctgcggttg agcttttggt tggaattcct tcggttgttt
    16081 atgggtttat cggtttgcaa gtgattgttc cttttatgcg ctctatcttt ggtggcacag
    16141 gttttggaat cctatctggg gtctgtgtct tgtttgttat gattttacca acagtgactt
    16201 ttatgacaac agacagtctg cgggcggtgc ctcgtcatta ccgcgaagcg tctatggcta
    16261 tgggagcaac acgttggcaa accatttggc gtgttgtcct taatgcagct cgtccaggaa
    16321 tttttaccgc tgttattttt ggaatggcga gagcttttgg agaagccttg gctatccaaa
    16381 tggtagtcgg taactctgct gtaatgccaa gttcactaac gacaccagcc gcaaccttaa
    16441 cgtctgtctt gacaatgggt attggtaaca cggttatggg aacggtccaa aataatgtgc
    16501 tttggtccct tgccctagtc ttattattaa tgagtctggc cttcaattcc cttgtcaaat
    16561 taatcacgaa agaaagaaag agaaattatg aacgctaaaa aagtcgataa agtagcaact
    16621 ggtactcttt ataccattgc tggaattatt gtagctattt tagcttcctt aattctatat
    16681 atccttgtcc gtggcttgcc acacatcagc tggtcctttt taacaggaaa atcgtcttcg
    16741 tacgaagctg gtgggggaat tggtatccag ttgtataatt ccttcttcct attgattgtt
    16801 accctgatta tttccattcc tttatcaact ggagcgggga tttacttggc tgaatatgcc
    16861 aaaaaagggc ctgttaccaa ctttattaga acctgtattg agattctgtc ttccctacca
    16921 tctgtggttg tagggctctt tggttacttg attttcgttg tgcagtttga atatggcttt
    16981 tctattattt caggggctct tgctttgacg gtctttaatc ttcctcaaat gacccgtaat
    17041 gttgaagata gtttactgca tgttcatcat acccaaagag aagcaggatt agctttaggc
    17101 ctgtcacgct gggaaacagt tttttatgtg gttatcccag aagctctccc gggaatggta
    17161 acaggtattg tcttagcttc aggtcgtatt tttggtgaag cggcagcgct tatctatact
    17221 gctggtcaat cagcaccagc tctggattgg tcaaattgga atccacttag tgttactagt
    17281 ccaatctcta ttttccgtca atctgagacc cttgctgttc atatttggaa agtcaatagc
    17341 gaagggacaa ttccagatgc tactcttgta tccgcaggaa gtgcagcagt gctattaatt
    17401 tttatcctta ttttcaactt ttcagctcgc tttattggga agaaacttca ttctaaaatg
    17461 acagcagcga aataagatat tggagaaatc atgacagaat ataattggaa tgaacgtcat
    17521 atcatcactt ttcctgaaga aacgcttgct ctagctacca aggatttaca tgtttactat
    17581 ggtgcgaaag aagctatcaa agggattgat atgcagtttg aaaaacataa aataacagcc
    17641 ttgattggtc catctggttg tggcaaatca acttatcttc gtagtcttaa ccgcatgaac
    17701 gataccattg atattgctcg ggttacaggt gagattcttt atcagggcat tgatgttaat
    17761 cgaaaagaca tgaatgttta cgagattcgc aagcacttag gaatggtttt tcaaagaccc
    17821 aatcctttcg ctaaatccat ttataaaaac attacctttg cccatgaaag agctggtgtg
    17881 aaagacaaaa aagttttaga tgaaattgtt gagacttcac tgaaacaagc cgcgctttgg
    17941 gatcaagtta aagatgacct tcacaaatca gccttcacct tatcaggggg tcagcaacaa
    18001 cgcttgtgta ttgctagagc catttctgtc aaacctgata ttctattaat ggatgaacca
    18061 gcctctgccc ttgacccgat tgcaacgatg cagctggaag aaaccatgtt tgaattgaag
    18121 aagaattaca cgattattat tgtgacccac aatatgcaac aagcagctcg tgccagtgat
    18181 tacactgcct tcttctattt aggcaacctc attgaatatg ataagacacg taatattttc
    18241 caaaatgcgc aatgtcagtc aacaaacgac tacgtttcag ggcactttgg ttagaaaggc
    18301 aatatgatga cagaacctat tttacagatc agagaccttt ctgtttacta caatcaaaag
    18361 aaaacgttaa aagatgtctc tttggacctt tatccaaatg aaataacagc tttaattggt
    18421 ccttctggat ctgggaaatc aaccctcttg cgttctatca accgtatgaa tgatttgaat
    18481 cctgaagtaa ccattacagg ctctatcgtc tataacggac acaatattta tagtccaagg
    18541 acagatacgg ttgatctacg caaagaaatt ggtatggtat tccaacaacc caacccattt
    18601 ccgatgtcta tttatgagaa cgtggtttac ggccttcgct taaaaggtat cagagataaa
    18661 tcaatcttag accatgctgt tgaaagctct cttaaagggg cttccatttg gaatgaagtc
    18721 aaagatcgtt tacatgattc tgcggttggc ttatcaggcg gacagcaaca gagagtttgc
    18781 atagctagag ttcttgctac cagccccaga ataatccttt tagatgaacc tacctcagct
    18841 cttgatccta tctcagctgg gaaaattgaa gaaacattat tgttattaaa aaaagattat
    18901 acactggcta ttgtgacccg ctcaatgcag caagcctcac gtttatcaga tcgaacaggt
    18961 tttttcttgg agggtgactt acttgagtgt ggtcctacaa aagctatgtt tatgaatcct
    19021 aagcgcaaag aaaccgaaga ctatatttct ggtaaatttg gttgagaaag acgtatatgg
    19081 ttgacccctc tgtgttttta ttataatgac tagattgtag gaagaaaaaa tcatgttaag
    19141 aacgaaattt gaagaagaat tagataaatt gcataaccag ttttattcaa tgggaacgga
    19201 agtattggca caaatcaata aaactgttcg tgcctttgtt agtcatgatc gtgaattggc
    19261 caaagaagtt atcgaagaag atgacactat taataatttt gagacaaagc ttgaaaagaa
    19321 atcgctggaa attattgccc tacaacagcc agtttcgaat gatcttagaa tggttattac
    19381 tgttttaaag gcatcaagtg atattgaacg tatgggtgac catgctgctt cgattgccaa
    19441 ggccactatt cgtatgaaag gtgaggagcg tattccagtt gttgaagaac aaatcaacct
    19501 tatgggaaaa gctgtgaaac agatggtaga agaagccctt aatgcatata tcaatgctga
    19561 cgatacaaaa gcttatgaaa tcgcagcttc tgatgagatt atcgatcaat atttccgtaa
    19621 tatccaaacc ttagctgttg aagaaatccg taagtctcca gatgctgttt ttgcaggaaa
    19681 agaatatttt caagtattaa tgtatttgga acgtattggt gactatgctc gcaacatttg
    19741 tgaatggatt gtttatctta aaactggaaa aattattgaa ttataagaaa ttatgctata
    19801 atgagactgg gaaacagctt gccagtccct ggttttatac gaaattgtgc aagttagaaa
    19861 atgaactaac aagaaaagtt ttctttgctg aatctgattt tgttaggtta acaaatatat
    19921 gtagacagag atgatactta ggtatcatct gttgatctat tatgatttgt tattagaatt
    19981 aaggagttat atgaagacag tagaacatct catcgaaaca tttgtgcctg aaaattataa
    20041 catcttttta gatattaatc gtcagactaa aacatttacg gggaatgttg cgattaatgg
    20101 tgaagcttta gataatcatg tggctttcca ccaaaaagat ttggacatta agtctatttt
    20161 gctcgataat gaagctgtta tttatcaagt ggataacgac aatgaagttg tgcgtgttga
    20221 attacctgaa acaggaatga tgacgcttgt cattgaattt tcaggtagca ttacagataa
    20281 tatgacaggt atttaccctt catattacac taaaaatggt gagaaaaaag aagtcatttc
    20341 aactcagttt gaaagccatt ttgcacgtga agctttccca tgtatcgatg aaccacaagc
    20401 aaaagctact tttgatttaa gcttgacctt tgatcaagaa attggtgaga tagcgttatc
    20461 gaatatgcca gaagtcaata ttgaccgtcg cgaggagact ggtctttgga catttgatac
    20521 gacaccaaga atgtcatctt acttgctagc gtttgcgcta ggagaacttc atggaaagac
    20581 tgtagaaagc aaaaaaggaa caactgttgg tgtgtatgcg acaacagcgc atcctcttag
    20641 ctcgcttgat ttttcattgg atattgcagt tcgtgttatc aacttttatg aagactattt
    20701 tggagtacat tacccaatcc cgcaatctct taatattgcg cttccagatt tttcatctgg
    20761 tgctatggaa aattggggtt taatcactta tcgtgaaatt tatttattag tagatgaaaa
    20821 ttcgactgtt caaagccgac aacaagttgc acttgtaatc gcccacgaga tagcacacca
    20881 atggtttgga aacttggtta caatgaaatg gtgggacgat ctgtggttaa atgagagttt
    20941 tgccaacatg atggaatatg tctctatcga ggctattgag ccgtcttgga aaataataga
    21001 agatttccaa acaggcggta ttcctcttgc tttaaaacgt gatgctactg atggtgttca
    21061 atcagtgcat gttgaagtca atcatccaga tgagataaat acattgtttg atcctgctat
    21121 tgtctatgca aaaggtagtc gtttgatgca tatgttacgc cgttttattg gtgatcgtga
    21181 ttttgccatt ggcttacatc actactttga aaaatatcaa tatcgaaata ctgtcgggcg
    21241 tgatttatgg aatattttat cagatacttc aggcaaagac gtagctgctt ttatggatgc
    21301 ttggcttgaa caaccaggtt atccagtttt aacagctcgc cttgaaaacg atcaattgat
    21361 tttgagtcaa aaacaatttt ttattggaaa aggtgaagaa aaaggtcgcc tttggccgat
    21421 tcctttaaac actaattggc atggccttcc tgaaactttg acagaggcag aaatggtcat
    21481 tccaaacttt agtcaattgg cagcagaaaa tgaaggagct ctacgtttca atattgataa
    21541 tacagctcat tacattacag attaccaagg atcattatta gatgccttag tcactgaact
    21601 tgcccaactt gataatactt ctgctttaca agttattcaa gaacgtcgtt tattggcaga
    21661 tagtggcttg atttcctatg ctgaattggt tgacttaatt gctcaattgg acgattctaa
    21721 gtcttatatg gtagcagaag cggttcagca agtggtctct ggtttgaaac gcttcattga
    21781 tgaaggaagc ttagcagaaa aatcctttaa tcgcttggta accaccatct atcaagaaga
    21841 ctttaatcag catggttttg agaaaaaagc tgacgaatca gatgaagatg agatggtacg
    21901 tcaggttgcc ttgggacgct tgtggctagc agaaaatcag acgattattg acggattgag
    21961 aactattttt gaagcatatc aaaacaatat tgcaagtatt ccagcagctg ttcgtcgctt
    22021 ggttttagcc aaccaaatga aatattttga aacagatagc ttggttgata tttattttga
    22081 gacttatgta gcaactactg ataataattt acgcagtgat ttaacggttg cttttagtca
    22141 aacaaaacaa tcaactacta ttagacgtat tcttgttagt ttaaaagata aagatattat
    22201 taaacctcag gatttatctt attggtacaa tgctttgctt ggacaaagct ttactcaaga
    22261 tatcatttgg gaatgggcta gagaaaattg ggattggatt aaatctgcac ttggtggaga
    22321 tatgagcttt gataaatttg ttatttatcc agcaagtaac tttaaaacac caaaacatct
    22381 agcagaatat aaatctttct ttgaacctaa gctagatgat atggctatta gccgtaatat
    22441 taccatggga attaatgaaa ttgaagcacg tgttgctttg atcactaaag aaaaagaagc
    22501 agttattgca gctctcagtc attactagac cataagcaaa agagttgggg ggactttcca
    22561 gctctttttt gtagtgagct agcttccttt cttagttccg tctataacta aatgattatc
    22621 catagtttaa ttcaaaagga gattgcactt taaagcgaag gctattagcc tcttaaatac
    22681 ttttttacca ggtggtgaat aagaacaatt ataaatgatt taatcattat gctttaaatg
    22741 ccttaaagga gtattaatat tttagtaaac cttaagcttt ttgtagtata gtctcgttag
    22801 aaattgttat cagaaatgag actaagttaa atgaagtgat agttacttta tttaagaaca
    22861 attgaggtag agacatgata aaaatattat tagtagaaga tgacttgagc ttatcaaatt
    22921 ccatttttga ttttttagat gattttgcag acgtcatgca agtttttgat ggcgatgaag
    22981 gtttatacga agcagaaagt ggtatttatg atttgatttt gttagacctt atgttgccgg
    23041 agaaaaatgg ctttcaagtc ttaaaagaat tgcgagaaaa agatattaaa attcctgtgt
    23101 tgatcatgac agctaaagag ggtttagatg ataaaggtca cggatttgaa ttaggtgctg
    23161 atgattatct gacaaagccg ttctatttag aagagttaaa aatgcgaatt caagcactgt
    23221 taaaaagaac agggaaattt gcagataaaa atattagttt tggcaattta gtggtcgact
    23281 tagctcgaaa agaggtgaag gttgaaggga aagtagttga attacttggc aaagaatttg
    23341 atttgttggt gtatcttttg caaaatcaaa acgttatttt accaaagaca caaatttttg
    23401 atcgtttatg gggattcgat agtgatacta cgatttcggt tgttgaagtt tacatttcta
    23461 aaataaggaa aaaattaaaa ggtacccgtt ttgttaacag gcttcaaact ttaagaagcg
    23521 taggatatat tctaaaaaac aatgaataaa ttaaaaaaag agattttatc agataactat
    23581 aaccactttt ttcatttttt tgcggttttt acaggtattt ttgtcattat gactattatt
    23641 atcttacaga ttatgcggtt tggcgtttat tcgtcagttg acagtagttt agtttctgtt
    23701 agtaataatg caagtagcta tgctaatcgt acgatggcta gaatatcttc tttttacttt
    23761 gatactgaaa ataacattat taaggcgctg cctgattcag atagttctaa gttattagga
    23821 acgcctgcag ctaatacaga tatcattttg tttagtgcta atggaacaat tttaaatgct
    23881 tttgatgcgt tttctaacta tcaaaatttt catttagata aacgccggtt ggggagtatt
    23941 gaaaccacca gtttaatgaa tttttatgga caagaagaaa aataccatac gataactgta
    24001 agggttcata tcaaaaatta tcctgcagtt gcctatatga tggcagtagt aaatgtggaa
    24061 caattagacc gcgctaatga acgttatgag cgcattatta ttatagttat gagtgttttt
    24121 tggctaattt ctattttagc aagtatttat ttagccaagt ggagcagaaa acctatttta
    24181 gaaagctatg aaaaacaaaa aatgtttgtt gaaaatgcta gtcatgaact aaggacccct
    24241 ttggcggtct tacagaatcg tctggaatcg ctttttcgta agcccaacga aacgatatta
    24301 gaaaatagtg agcatctcgc ttctagttta gacgaggttc gcaacatgcg catcttaaca
    24361 actaatttat taaatttagc aagacgagat gatggcatta atccacagtg gactcattta
    24421 gatacagatt tttttaatgc tatttttgag aattatgaac tagttgctaa agaatatgga
    24481 aaaatatttt attttcagaa ccaagtcaat aga
//
LOCUS       DAUCWO010000024        24436 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUCWO010000024 DAUCWO010000000
VERSION     DAUCWO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.024"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            287..1330
                     /gene="pheS"
                     /locus_tag="VGY20_000880"
     CDS             287..1330
                     /gene="pheS"
                     /locus_tag="VGY20_000880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ4273747.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            1540..3945
                     /locus_tag="VGY20_000881"
     CDS             1540..3945
                     /locus_tag="VGY20_000881"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ4273748.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDK
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDGNS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIRRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQVVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALSSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            4056..4433
                     /locus_tag="VGY20_000882"
     CDS             4056..4433
                     /locus_tag="VGY20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ4273749.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            4426..4812
                     /locus_tag="VGY20_000883"
     CDS             4426..4812
                     /locus_tag="VGY20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ4273750.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFYCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            4885..5961
                     /locus_tag="VGY20_000884"
     CDS             4885..5961
                     /locus_tag="VGY20_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273751.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAIDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNIVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            5971..6639
                     /locus_tag="VGY20_000885"
     CDS             5971..6639
                     /locus_tag="VGY20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273752.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSHLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGIARLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLEYCDKVYRMQDGEL
                     RQER"
     gene            complement(6743..7660)
                     /locus_tag="VGY20_000886"
     CDS             complement(6743..7660)
                     /locus_tag="VGY20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ4273753.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            7811..11026
                     /gene="rexB"
                     /locus_tag="VGY20_000887"
     CDS             7811..11026
                     /gene="rexB"
                     /locus_tag="VGY20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ4273754.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLATYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            10987..14655
                     /gene="addA"
                     /locus_tag="VGY20_000888"
     CDS             10987..14655
                     /gene="addA"
                     /locus_tag="VGY20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ4273755.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNVLVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKFTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFGE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLSLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QIPQLLNADDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKATYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALRGIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            14795..15607
                     /locus_tag="VGY20_000889"
     CDS             14795..15607
                     /locus_tag="VGY20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4273756.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHHERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            15748..15924
                     /locus_tag="VGY20_000890"
     CDS             15748..15924
                     /locus_tag="VGY20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ4273757.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(16052..16414)
                     /gene="mscL"
                     /locus_tag="VGY20_000891"
     CDS             complement(16052..16414)
                     /gene="mscL"
                     /locus_tag="VGY20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ4273758.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            16544..18358
                     /gene="dnaG"
                     /locus_tag="VGY20_000892"
     CDS             16544..18358
                     /gene="dnaG"
                     /locus_tag="VGY20_000892"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ4273759.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRMPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            18367..19476
                     /gene="rpoD"
                     /locus_tag="VGY20_000893"
     CDS             18367..19476
                     /gene="rpoD"
                     /locus_tag="VGY20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ4273760.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            19713..20051
                     /locus_tag="VGY20_000894"
     CDS             19713..20051
                     /locus_tag="VGY20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ4273761.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            20189..21043
                     /gene="rfbD"
                     /locus_tag="VGY20_000895"
     CDS             20189..21043
                     /gene="rfbD"
                     /locus_tag="VGY20_000895"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ4273762.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEYFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            21162..22316
                     /locus_tag="VGY20_000896"
     CDS             21162..22316
                     /locus_tag="VGY20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ4273763.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYEAAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDHLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            22306..23238
                     /locus_tag="VGY20_000897"
     CDS             22306..23238
                     /locus_tag="VGY20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4273764.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMAAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            23241..24044
                     /locus_tag="VGY20_000898"
     CDS             23241..24044
                     /locus_tag="VGY20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273765.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            24044..>24436
                     /locus_tag="VGY20_000899"
     CDS             24044..>24436
                     /locus_tag="VGY20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273766.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDM"
BASE COUNT         7820 a         4265 c         4972 g         7379 t
ORIGIN      
        1 ggcgtggaaa agtcactgat ttaagattgt cactaagcac tttttttgat agaatagaac
       61 taattgaata agtgtctatg agactagtac taatagagaa attaacaggg agtgagggcc
      121 gtgactggaa gccttcttaa gggaactatt aggaattcac tcagctaaag acaattaatt
      181 aaggtctgat aacatcagat aaaaacggat ggtaccgcgt gtcaacgctc cgcttatgaa
      241 gtggagtagt ggcacgcttt tttcatcgct aaaagaaggg aaaactatgg atttacaagc
      301 acaattagag gaattaaaaa caaaaacatt ggagactctc caatctttga cggggaatca
      361 taccaaagaa ctgcaagatt tgcgcgtggc tgttcttggg aaaaagggat ctttgacaga
      421 attactaaag gggctaaaag acttgtctaa tgacttgcgt cctgtggttg gtaaacaggt
      481 gaacgaagtt cgtgacttat tgacaaaagc ctttgaagaa caggctaaaa ttgtagaagc
      541 tgctaaaatt caagcccagc ttgatgctga aagcattgat gtgactttac cgggtcgtca
      601 aatgactctg ggccaccgtc atgtgttaac acaaaccagt gaagaaatcg aagatatttt
      661 cttaggaatg ggttttcaga ttgttgatgg ctttgaggta gaaaaagatt attacaactt
      721 tgagcgcatg aatttgccaa aggatcaccc tgcccgtgac atgcaagata cgttttatat
      781 cacagaagaa atcttgttac gaactcacac cagtcctgtg caggcccgta ctcttgatca
      841 acatgatttt tcaaaagggc ctttaaaaat ggtttctcca ggtcgtgttt tccgtcgaga
      901 caccgatgat gctacccact cacatcaatt ccatcaaatt gaaggtttgg tcgtgggcaa
      961 aaatatttcc atgggagacc tcaaagggac tcttgaaatg atcatcaaga agatgttcgg
     1021 tgaagaacgt agcattcgtt tgcgcccgtc atatttcccg ttcacagaac catctgttga
     1081 agtggatgtt tcttgcttta aatgtggtgg gaaaggctgt aatgtctgca agaaaacagg
     1141 atggattgaa attcttggtg ctggtatggt tcacccaagt gtcttggaaa tgtcaggtgt
     1201 tgatgcaaaa gagtattcag gctttgcctt tggtttaggt caagagcgga ttgccatgtt
     1261 gcgttatggc attaatgaca ttcgtggttt ctatcaaggc gaccaacgtt tctctgaaca
     1321 atttaactaa aatgagtcgt ttgaagactt ctgtcagcta ttttatctaa gattgagtga
     1381 aagttgatag gtaatgataa taggttaatt gtctaagggg aaagtccctc tcgcttcaac
     1441 gtcataaggg ttttgacttg gaacttaaaa tacgaaagga aatgactcaa atcacggctt
     1501 gagagctttc tctagccgag tgatttgggc actaaaatta tgttagtatc ttataaatgg
     1561 ttaaaagaat tagtagatat cgatgtgaca ccggcagcac ttgctgagaa aatgtcgaca
     1621 acgggtatcg aggtagaagg aattgaagtc cctgcagatg gtttatcaaa actggttgtg
     1681 gggcatgtgc tttcttgcga agatgtgcca gagacacatt tgcatttgtg tcaggtagat
     1741 acaggtgatg aaacgccacg tcaaattgtg tgtggcgcac caaatgtgaa agcagggatt
     1801 aaggttatcg tggctgtgcc aggtgctcgt attgcggata actataaaat caaaaaaggc
     1861 aaaatccgcg gcatggaatc tcttggcatg atttgttcct tgcaagaact tggtttgtca
     1921 gatagtatta ttccaaaaga attttcagat ggtattcaaa tcttgccaga ggaggctgtg
     1981 ccaggtgatg ctatttttaa atacctggac ttggacgatc atatcattga attgtccatt
     2041 acacctaacc gtgctgatgc gctttctatg cgaggtgtgg cacatgaagt cgcagctatt
     2101 tatggcaagt cagtatcatt cccccaaaaa aatctacaag aatctgataa ggcaacttca
     2161 gaagccattg aagtggccat tgcctctgat aaagttctaa cttacgctag ccgtgtcgtt
     2221 gaaaatgtca aagttaagcc aagtccacaa tggttgcaaa accttttaat gaatgctggt
     2281 attcgtccta ttaataatgt ggttgatgtg accaactatg tgctcttgta ttttggtcaa
     2341 cctatgcatg cttttgatta tgataaattt gaagaccata agattgtggc gcgtgcggct
     2401 cgtcaagggg aaagtttagt cacacttgat ggtgaaaaac gggatttgac cactgaggat
     2461 cttgttatta ccgtggctga caaaccagtt gctttagcag gtgttatggg tggtcaagcc
     2521 actgaaattg atggcaactc tcagacggtt gttcttgaag ctgctgtttt tgatggcaaa
     2581 tcgattcgta aaaccagtgg tcgtttaaac cttcgttcgg aatcctcttc acgttttgaa
     2641 aaaggggtta actatgcaac tgttttagaa gcgcttgact ttgcagcggc tatgttacaa
     2701 gaattagcag aaggtcaagt cttatcaggt catgttcaag ctggacaatt gccaacagaa
     2761 cctgttgaag tctcaactag ccttgactat gtgaatgttc gtttaggaac agaattaact
     2821 tttgctgata ttcgaaggat ttttgaccaa cttggatttg gtctaacagg agatgaaaca
     2881 agcttcacgg tagctgttcc tcggcgtcgt tgggatgtta gcatcccagc tgacttggtg
     2941 gaagaaattg cgcgtatcta tggttatgat aagttgccga caaccttacc agaggcaggg
     3001 ggaacggctg cagaattaac gccaacacag gctcttcgtc gtaaagtccg tggcttagcg
     3061 gaaggactgg gcttgactga aatcatttcg tatgccttga caacaccaga gaaagctgtt
     3121 gagtttgcag tggcaccaag tcacctaaca gaactcatgt ggccaatgtc tgtggaacgt
     3181 tcagcgcttc gtcaaaatat ggtttcaggg atgttagata cggtagctta taatgtggct
     3241 cgtaaacaaa gtaacttggc tctttatgaa attggaaaaa tctttgaaca agaagctaat
     3301 ccaaaagaag accttcctaa tgaagtgaac cattttgctt ttgctatttg tggcttagtg
     3361 gcacaaaaag atttccaaac ccaagcacag gtagtggatt tttaccatgc taaaggtatc
     3421 ttggatactt tatttgcaaa ccttaacctc aaggttcaat atgtgccaac caaagaccta
     3481 gcaagcatgc acccaggacg gacagcgctt atcctattag atgagcaagt gattggattt
     3541 gttggtcaag ttcatccagg aacagcaaag gcttatagta ttccagaaac ctatgtggca
     3601 gaattggata tggcggcttt agaagcagcg ttgtcaagtg atcaaacttt tgtggagatt
     3661 accaaattcc ctgctatgac acgtgatgtt gccttgttac ttgaccgtga agtgagtcat
     3721 caggccattg taacagctat tgaatcagca ggtgtaaaac gcttgactaa gattaagctc
     3781 tttgacgtgt atgaaggagt aactattcag gctggcaaaa aatcaatggc ttatagcctt
     3841 actttccaaa atccaaatga caatttaacg gatgaagaag ttgctaaata catggagaaa
     3901 atcaccaaag cattgacaga acaagtcggt gctgaagtac gctaagaaaa aaaactgggc
     3961 ctctaaggct ccgttttttt ctgttataat agaggaaaaa agaactccag ttcaaatgat
     4021 tggagaaaaa catcagaagg tggaggaaaa tcagaatgta tcaaacaaaa ataacaggca
     4081 accgccttta ccacacggtt tcgaaaggct acggagatag tgtaacctta tttggaaaaa
     4141 ctgatcaagg ggaaacccct atgagtttac tggttattgc cttgtcttct tgtgtgacta
     4201 tgtgtgtgca gggtttttac catcgtcaat ttcagcaaga cacacttgct gtttctgttg
     4261 aggctagtta tgaggaagaa gtttttgaac tacttgttag gattgatgat gttttagatg
     4321 acaaaaagaa agctgattta ttggcttaca ttggaactta ttgtcgtgtg aaacaactcc
     4381 tcaaacctga agttacggtg accattacgt tggaaggact gtctcatgtc tgatgctagt
     4441 ttgaaaactt accaaaaaca atgggcttat caaaagtatt gggtcatggc acactcacag
     4501 cagcattaca atgctttaag agaattgttt aaaggaaatc agtggtcaga agaaaaggtg
     4561 ttgacttttt attgtttgat tgaagaagcg caagccattc ctcccacagt aaaaagctta
     4621 cgaacggcat atcagcatgt ttgggggtat tttaaaaagg tagcgagtca ggaagaaaaa
     4681 gaccatttca aagatttgga tgctcagttg gaaactaaat ctgaggagat gctatgcttt
     4741 ttacaagaga tgactgctca ttatcagccc tcttatctgc tatcttgccg tttgattacc
     4801 aaaggtcctt aaatgctaaa cagaaaagta gggatttctg ggaagttatg ctaagctagt
     4861 gatataaata agggggaatg agagatgttt ttagcgctta atgaaatgaa acaatccaaa
     4921 ttgcggtatg gtttgattgc tggtttgcta tgtttggttg cctatttgat gttctttttg
     4981 tcaggattgg cctttggttt gatgcaggag aaccgctcgg cgattgatct ttggaaagct
     5041 gacagtgttt tactagccaa ggatgctgac gctaccttga ccttatcaca ggtgtccaga
     5101 gctcaagaaa atcaaataac agcagacaag gtagctcctc ttgctcaact caatatcgtg
     5161 gcgtggtcag ttaaaaatcc taaggatgcc gacaaagtta aggttagcct tttcgggatt
     5221 gattctaata gctttattcg tcctaacatt gtaaaaggtc gattatttaa gactaacaaa
     5281 gaggttgttt tggatcaaag ccttgcaaaa gaggaagctt ttgcgattgg caaggacttt
     5341 tacacatcga gttctagtca agcattaact atcgttggtt atactcaaaa tgctagattt
     5401 agtgttgcac cagtggttta tatgaatttg gaagcttttg aaacattaaa atatggagaa
     5461 ccactaccaa aagataagca agttgttaat gcttttatca ctaaaggaag tttaacagat
     5521 tatcctaaaa aagacttcca aaaattagat attaaaacct ttattactaa attacctggt
     5581 tatagcgctc aacttttaac ttttggcttt atgattagtt ttcttgtcat tatttcagct
     5641 attattattg gtatttttat gtatattttg actattcaaa aggcacctat ttttgggatt
     5701 atgaaagcgc aaggaatttc taacaaaacg attacgaccg ctgtgcttat gcagacattc
     5761 tttttgagtt ttttaggtag tggtttaggg ttgctaggta cttggctgac atcattatta
     5821 ttaccaacag tagtaccttt tcaaagcaat tggtttttgt atttggctat attcgttagt
     5881 atgatctgtt ttgctctatt aggcacctta ttttctgttt ttaacattat acgaattgat
     5941 cctttgaaag caattggata ggagatagtg atgtcggttt taacatttaa acaagtgacc
     6001 aaaacatttc aagatggtca tcacgaaatc aacgcattaa aggcaactga cttttccatt
     6061 gaagctggag aatttgtggc tattattgga ccatcaggtt ctggaaaatc aacctttttg
     6121 accattgctg gtggcttaca gaccccaagc tctggtcagt taatcattga tggcactgat
     6181 tatacgcatt tatcagaaaa agagcgctca cacttgcgct tcaaatccgt tggttttatc
     6241 ttacaagctt ccaatctgat acctttttta acggtccagc agcagttgga attggtcgat
     6301 catttgacag gaagcaaaga aaaagccaaa gccaatcagt tatttgatga tttaggcatt
     6361 gcaaggctaa aacatcagtt acctcaagaa ttatccggcg gagaaaggca aagagcggct
     6421 attgcccgtg ctctttacca tgatccagca ctaatattgg cagatgaacc tactgcaagt
     6481 ttggatactg aaaaagccta tgaagtggtc aaactgttgg ctaaggaaag taaagaaaaa
     6541 aacaaggcca ttattatggt cactcatgat gatcggatgc tagaatattg tgacaaagtc
     6601 tatcggatgc aagatggaga gttacgtcaa gagagatagt agactggtaa gagtttatga
     6661 tattagaaga aaaaaccaaa caagcacagc acttgtctgg tttttttcta agtgtgacta
     6721 ttatcaatac gtcaccattt ccctaaagct tactatacgg gatgctaaag gtatcaccat
     6781 gctcaaagtc gccatattgg taacctcggt caaaccaacg ttggcgttgc ttagaagttc
     6841 cgtgggtaaa actatcagga acggctctgc cataggtttc ttcttgcaga gtatcatccc
     6901 caactgcgtg agctgctgcc attgcctcct caatgtctcc tttttccaaa aggccttgcc
     6961 cttgaacata attagcccag gcaccagcat agtaatcagc ttgtaattcc aacttgacgt
     7021 tgagctgatt ggctttagcc tttgattttc cttgtctagc gctagcgtaa ttatccataa
     7081 ttcccaattc attttggatg tggtggccaa cttcatgagc aatcacatag gccatagcaa
     7141 aatcaccttt tgcgccgtac ttggttgata gttcattata aaaagaaata tctaaataca
     7201 ctttttgatc gcctgggcaa tagaaaggac cagaagaggc ttgccccctg ccacaagcgg
     7261 tttgagttgc tccagtatat agaactaatg ttggtttatg ataagttaac cctttctcac
     7321 gaaatgtctt agtccaatag tcctcggtag aggcgaagac cttactgaca aaagaaacct
     7381 gttcttgact agccttgtca ccatttgtac gcgttacttg gcttgattga taggcattgt
     7441 tgttggtaga agaaggcttg cctcctaaaa caccagatag gccacctcct cccataacta
     7501 ggagaaggag aagaataacc agcttggttt tccaacctcc tcgtgaaaag agtaattgaa
     7561 gcaggagacc gccccctagg ccgccgcccc caaaagaacc agacgattga ccacgtctat
     7621 cttcaacctg ttgactttct ctcaaatcat ctgttttcat aagtttctcc catctatttc
     7681 ttgattttat tataaaataa ttctattaga attgctattg ttatgatgag attataacta
     7741 aaaataggaa aagcaatagg tatttgctat aatataacaa gggtagattc agtttgtata
     7801 aaggagtcat atgaaattaa tttacacaga gatgagttac tcgatgacag agattttggt
     7861 gaatgaagca cgaaaagctg ctgaccaagg ttatcgagtg ttctatattg ctccaaattc
     7921 tctttcattt gaaaaggaaa gagaagtctt aaccttattg cctgaacgag ggactttttc
     7981 gataatagtc acccggttcg ttcaaatgtc aaggtatttt acagttgaga gctctccttc
     8041 taagcaacat ctagatgaca cgactttggc tatgattttt tatcgggcct tgatgcaatt
     8101 aaaaccagaa gatttgccga gttatggtcg tttgcaaaat aattcggttt ttattgaaca
     8161 actagttgag ttatataagg aattaaaaaa cgcacaatta agcgtacatg atttaacagg
     8221 attagaccat cctcaaaaac aagaagatct tattaaaatt attgagcttg cagagacgat
     8281 catgattcag caagattata atcaagatag tcctctacaa tcttttgcaa gagcaattaa
     8341 gttaggactt ttaaataacc aactatcaaa aacagttgtt gttattgatg gtttttcacg
     8401 tttttcagct gaagaggatt atttattatc acttttaaat aacaactgtc aagaggtcat
     8461 cattggcagt tatgtgagtc aaaaagctta tcaaaaatct tttattaagg gaaatatcta
     8521 tgaggctagc cttcattttt tgcaagattt ggctcaaaaa taccatatca agccggtatt
     8581 tgcgacctct aatcaagtgt ttaaaccagc cttttcacgg ctaacacaat tatttgaagc
     8641 aactcatgat ttttcacaag tagactggca actccaaaag aatgatcttg atcactttag
     8701 cctttggcaa tgtcatcatc aaaaagagga aattgaacat gtagcaaaat ccattcggca
     8761 aaagctatat gaaggttacc gttataagga tatcttagtg ttgctaggag atatggatgc
     8821 ttatcaatta caaattgggc ctatctttga taagtttgaa atcccctact atttaggaaa
     8881 agcagagcct atggctgccc atccattagt ccaatttata gaatcgctcg aacgaagtca
     8941 gcgctataat tggcgtcgtg aagacatttt aaacatgtta aaatcaggac tttttggttg
     9001 ttttgatgat agtgatattg atcgctttga ggaatatact cagtttgcag atattaaagg
     9061 gtttactaaa ttttcaaagc cttttaccat taactcttct aggcagtatc ctcttgactt
     9121 tttaaatgaa atgagacaag acatcgtttt acctttgcaa gaattgttta agagtcaaaa
     9181 acaacttggt gcttcacttg ttgacaaatt aatacttttt ttaaaaaaga ttagattagc
     9241 tgagaatatg caaggtttag cacagtccca attagaggtg gaaaaaaacg aagaagtttg
     9301 gaaaaggttt acagatattt tgacttcttt tcatcatatc tttggccaag aaaaattacg
     9361 gctatcagat tgccttgctt taatcaaaac gggaatgaaa tcagctcagt atcgtgttgt
     9421 tccagcgaca ttagatgttg tcactattaa atcttatgac cttgttcaac cacattcaaa
     9481 acctttcgtt tatgcaattg gtctaactca atcacatttt cctaagcaaa ttcaccattc
     9541 aggactactt tctgatcaag agcgtgctag aattaatgaa ataagaaatt atcgtcattt
     9601 tgatattgct agtgccgaaa atagtaaaaa aaaccatcaa actgcgttat ctctgtttaa
     9661 tgctgctact aaagagttag ttcttagtgt tccaacggta atcaacgaga cattcgatga
     9721 tctatctcct tatcttaaag aattaataag ctttgggcta cctcttttgg ataaaggcaa
     9781 aaactatcta tcttatgata atagcgatat tggtaactat aaagctctct tgtcacaaat
     9841 tattgctatt aaccgacaag atcttataga aatgtcagat caagataaga tgttttggac
     9901 tgttgtctta cgttatctca ggaaacagtt aagaaagcaa cagctagagc tacctacttc
     9961 agattatcgt ttatcaacaa agcctttgtc taaagaagtt atcgaggtat gttttccaaa
    10021 gggaatccct ttaaagctat ctgcaactgc tttaaccgtt ttttataata atcagtacaa
    10081 ctattttttg aaatatgttt tgaatttaaa caaaacagaa tccattcatc cagattccag
    10141 aattcatggt caatacttgc atcgtgtctt tgagcggtta atgaaagatc acacgcaaga
    10201 accgtttgat aataaattaa aacaagcaat ctaccatacc aatcaagaaa gtttttttca
    10261 gcaggtttac caagataatg ccgaggctga gtacagttta gctatactag aagatattgt
    10321 tagatcaaca gcacctatct tgcaacttaa ccaaaacata aaggtcattg atcaagaaaa
    10381 gaactttcat ctggatatgg gaaatgagat attagtccac gggatcattg atcgtattga
    10441 ccagctgagc gacggtagct tgggcattgt tgactataaa tcaagcgcga atcaatttga
    10501 tattgggacc ttttataatg ggttgagtcc acaactagcg acttacctcg cagccttaaa
    10561 acagatcgca ccgcacgata taaaccaatt atttggggct atgtaccttc atttgcaaga
    10621 tcctaaattg gatctagtaa catttaaaca aattgataac acattagtgg agagtatcta
    10681 taaagcgttg acctataaag gtattttttc agaagtcgaa aaagagcatc tatctactgg
    10741 tgcttaccaa accaaaaatg ctctttattc caatgacgaa ttggaaacac ttttaaatta
    10801 taacaagtat ctctatctta aagctgctaa acacattaaa aagggacatt ttttgattaa
    10861 tccttataca agcgatggta agacagttca aggagatcaa ttaaaagcca ttacccgttt
    10921 tgaagcagat cttgacatgg cacaggcaag gcgactagtg actctccctg ctaaggagaa
    10981 aaaagagtgc tttttaacat taatgagaaa ggagagccac ttgtgatttc ttttgcccca
    11041 tttttaagcc ccgaagctat taaacattta caagaaaacg aaaggtgcag tgatcagtct
    11101 caaaaacgca cagctcaaca aattgaagca atttatacta gtggccaaaa tgtacttgta
    11161 tcagcttctg ctggttcagg aaaaaccttt gtaatggtcg aacgcatact tgataaaatt
    11221 ttgagaggtg tttcaattga tcggcttttt atctcaacct ttactgttaa agcagctaca
    11281 gaactgcgtg aacggattga aaacaaatta tactcacaaa ttgctcaaac tacagatttt
    11341 caaatgaaag tttatttaac agaacaattg caatctcttt gtcaagctga tattggtact
    11401 atggatgctt ttgcacagaa agtagtaagt cgctatggtt atagcattgg catttcatcc
    11461 caatttcgta tcatgcagga taaagcagaa caagatgttt taaagcaaga ggtgtttagc
    11521 aaactcttta gtgagtttat gaatcaaaaa gaggcaccgg tgtttagggc tcttgtgaaa
    11581 aatttttctg gtaactgtaa agacacttca gcttttagag agttagttta tacttgttat
    11641 tcttttagcc aatcgacaga aaatccaaaa atatggttgc aagaaaattt tctaagcgct
    11701 gctaaaactt accaaagact tgaagatatc ccggatcatg atattgaact cttacttttg
    11761 gcaatgcaag acactgcaaa tcagctaaga gatgtgactg atatggaaga ttatgggcag
    11821 ctgactaagg caggtagccg atctgctaaa ttcactaaac acttaacgat catagaaaag
    11881 ttgtctgatt gggtgcgtga ttttaaatgt ttgtatggaa aagccggatt ggatcggttg
    11941 atcagagatg tgacaggcct tataccatct gggaatgatg ttacagtctc gaaggtaaaa
    12001 taccctgttt ttaagacctt gcatcaaaaa ttaaaacaat ttaggcattt agaaacaatt
    12061 ttaatgtatc agaaagactg ttttccctta ttggaacagt tacaagattt tgtgcttgcg
    12121 ttttcagaag cttatttagc tgtcaaaata caagaaagtg cttttgaatt ttcagatatt
    12181 gcacactttg caatcaaaat tttagaggaa aatacggata ttcgccaatc ctatcagcaa
    12241 cactatcatg aggtgatggt tgatgaatat caagataaca atcatatgca agagcgactc
    12301 ctgaccttac tatcgaacgg tcataatcgc tttatggtag gagatatcaa acaatcgatc
    12361 tatcgatttc ggcaagccga tcctcagatt tttaatcaaa agtttagaga ctatcaaaaa
    12421 aaacctgagc aggggaaagt gattttactc aaagaaaact ttcgtagcca atcagaggtg
    12481 ttaaatgtca gcaacgctgt ttttagtcac ttaatggacg aatcagtagg agacgtctta
    12541 tatgatgagc aacatcagtt aatagcaggt agtcatgctc aaacagtccc ctatctagac
    12601 cgtcgtgctc agttattgct atataatagc gataaagatg atggcaacgc cccttcagat
    12661 agtgagggta tttcatttgg cgaggttaca attgttgcca aagaaattat taagcttcac
    12721 aatgataagg gtgtcccttt tgaagacatt acgttactcg tttcttcaag aacaagaaat
    12781 gatatcattt ctcatacatt caatcaatat ggtattccta tagtaacaga tggtgggcag
    12841 caaaactatc ttaaatctgt tgaagtgatg gttatgttag atacattacg caccattaat
    12901 aacccaagaa atgattatgc ccttgtggct ttactgcgct caccgatgtt tgcctttgat
    12961 gaagatgatt tagcaagaat agcacttcaa aaagacaatg agctagataa agattgccta
    13021 tatgacaaga tacaaagggc tgtgattgga agaggtgctc atcctgaatt gattcacgat
    13081 accttgcttg gcaagttaaa tgttttttta aagacgttga aaagctggcg tcgatacgct
    13141 aagctagggt cgttgtatga cttgatttgg aaaattttca atgatcgttt ttattttgat
    13201 tttgtagcta gtcaagcaaa agcagaacaa gcacaagcta atctatacgc attagctcta
    13261 cgtgctaatc agtttgaaaa atcgggctat aaaggactat accgttttat taaaatgatt
    13321 gataaggtac ttgagacgca aaatgactta gcagatgtgg aagtggctgc tcctaaacaa
    13381 gctgttaatt taatgaccat tcacaagtct aaaggtttac aatttccgta tgtatttatc
    13441 cttaattgtg acaagcgctt ctcaatgaca gatattcata aatcatttat tctgaatcgg
    13501 cagcacggta tcggtatcaa gtaccttgca gatatcaaag gtttacttgg tgaaacaaca
    13561 ctcaattctg ttaaagtaag catggaaacc ttaccttatc aattgaacaa acaagagtta
    13621 cgcttagcaa ctttatcaga acaaatgcgc ttactgtatg ttgctatgac acgagctgaa
    13681 aaaaaagttt attttattgg taaagctagt aagagcaaaa gtcaagatat cacagatcct
    13741 aaaaagttag gcaaactttt gtcgctggct ttacgagaac agttattgac attccaagat
    13801 tggctattag caatagcaga tatattttca actgaagatc tttattttga tgttcgcttt
    13861 attgaagata gtgatttgac acaagagtca gtcggacgac ttcagatacc acagttatta
    13921 aatgcagatg atcttaaaga taatcgtcaa tcagaaacaa ttgcacgggc tttagatatg
    13981 ttagaagcag tgtctcaatt gaatgccaat tatgaagcag ctattcattt gccaacagtt
    14041 cgaacgccta gccaacttaa ggcaacttac gagcctttat tagaacccat tggtgtagat
    14101 attatagaga aatcttctcg atcgctatct gattttactt tgccacattt ttcaaaaaaa
    14161 gcaaaagttg aagcaagtca tattggatca gctcttcatc agttgatgca ggtgctccct
    14221 ttgtcaaaac cgataaatca acaaacgctt ttagacgctt taagaggaat tgatagtaac
    14281 gaagaggtaa aaacagctct tgatctcaaa aaaatagagt cgttcttttg tgatacaagc
    14341 ctaggccaat tttttcagac ttaccaaaaa cacttgtatc gagaagcgcc atttgctatt
    14401 ttaaaagttg accctattag tcaagaagaa tatgtcctac gtggtattat agatgcctac
    14461 tttttgtttg atgatcatat tgtattagtg gactataaaa ccgataaata caagcagccc
    14521 attgagttaa aaaagcgtta ccaacaacag ttggagttat atgcagaagc tctcactcaa
    14581 acgtataaac ttcctgtgac taagcgctat cttgttttaa tgggaggtgg aaagccagaa
    14641 attgtcgaag tttaaggttg ttgtgacaca aggggatttt gcaaatgata atagtagagt
    14701 tgaggatttt acgaaatact tgaaaagcta ttgacctttg aataataata caatataata
    14761 agttttatta gcgacaaaaa ggaataaata tactatgaat tttaaaaaaa taagatttgg
    14821 ttttttgctt tgcctcctac tttatccttt agcagcctgt aacaagtctg aacaacttaa
    14881 tcaccacgaa agaattaaaa aaacaagaaa attagtcgtt gctgtaagcc ctgattatgc
    14941 tccttttgaa tttaaagctt tagttaatgg aaaagatact attgttggag cagatgttca
    15001 attagctcag gctattgctg atgaattaga cgttgatctt gagctttcac caatgagttt
    15061 tgataatgtt ttgtctagtt tacagactgg taaagctgat cttgctattt caggaatctc
    15121 acatactaaa gaaagagcta aagtatatga tttttcaatt ccttactatc aggcagaaaa
    15181 cgctatagtg atgagagcat ctgatgctaa agtgaccaaa aatattagtg acctaaacgg
    15241 taagaaagta gccgctcaaa aaggtagtat cgaggaaggt ttagttaaaa tacaattacc
    15301 aaaggccaat ctgatttctt tgactgctat gggagaagcc attaatgaac taaaagcagg
    15361 acaagtctat gcagttacct tagaaacacc tgtagctgct ggttttttag cccaacataa
    15421 ggatctggct ttagcaccct ttagcttaaa aaccagtgat ggagatgcca aagcagtggc
    15481 ccttcctaaa aatagtggtg acttaaccaa agccgttaat aaggttattg ctaagttaga
    15541 tgagcaagaa cggtacaagt catttattgc agagacaata gcactaacga aaaatactat
    15601 gaagtaataa ggttaataaa aactttatta gtatccgttg gaaaagtgtt tggaataaac
    15661 aataaaagag gtttgacttt ttttaaaaaa taatgtaata taattgggaa cgtgaaaatg
    15721 ttacaatatc ttgaggaggt gaaacacatg tcaaaaacag tagtacgtaa gaatgaatct
    15781 cttgacgatg ctcttcgtcg cttcaaacgt tctgttacta aagctggtac tcttcaagaa
    15841 tcacgtaaac gtgaattcta cgaaaaacct tctgtaaaac gtaaacgtaa atcagaagca
    15901 gctcgcaaac gtaaaaaatt ctaatggtaa aaaataaaaa cagttctcac aattagaact
    15961 gtttttattt tttatccaga agaagcaagg gagctttttt gatatcaaaa aaagcagtta
    16021 gacatgccta actactttat caaaaatcga tttatttttg agccaacaaa tcacggattt
    16081 ctgtcaataa ttcttcttga gtaggagctg cagcttcttt ttctttttta ggcatagctt
    16141 tttcagcggc ttttacaaca aagaaaagac tggtaccgat gataagaaaa ttaataacgg
    16201 ctcctaaaaa gctaccatat ttaacaccat tccatgacaa ttgagtgatg ttttcaacat
    16261 ttgcagcttt cagagctggg tttaaaataa gcggtgtgat aatatcgttc acaaaagaag
    16321 tgacaatagc accaaaagct cccccgatga taactgctac agcaagttca ataatgtttc
    16381 ctctaaacaa aaatgctttt aattctttaa ccattaaata tccccccttt taatatctct
    16441 aagttaagca tagcataatt agtaaacaaa atccaatgga atcacttcag tttttaaatt
    16501 tacaaaaaag agtttcggtg gtataataaa acgtaaaact aaaatgggat ttttatgggg
    16561 aggtgacgat ttggcaattg acaaagaaat gatttcccaa gtaaaaaata gcgttaatat
    16621 tgtcgatgtc attggagaag tggtcaaact ttcccgatca gggcggcatt atctcgggct
    16681 ttgcccattt cataaggaaa agacaccctc ttttaatgtt gttgaagaca gacaattttt
    16741 tcactgcttt ggctgtggaa aatcagggga tgtttttaaa tttattgagg aataccgcca
    16801 agtccccttc ttagaaagtg ttcagattat tgccgataag actggtatgt cgcttaatat
    16861 accgccaagt caggcagtac ttgctagcca acacaagcac cctaatcacg ctttgatgac
    16921 acttcatgag gatgctgcta aattttacca tgcagttttg atgaccacta ccattggtca
    16981 agaagctagg aagtaccttt accagagagg cttggatgac caattaattg agcatttcaa
    17041 tattggttta gccccagatg agtcagatta tctttatcaa gctctttcta aaaaatacga
    17101 ggaaggtcaa ttggttgctt caggattgtt taacttgtcc gatcaatcta ataccattta
    17161 cgacgccttt cgaaatcgta tcatgtttcc cttatcagat gaccgagggc atattattgc
    17221 cttttcagga cgtatctgga cggcagctga tacagaaaag agacaggcca agtataaaaa
    17281 ttcaagagga acagttcttt ttaacaaatc ttatgaattg tatcatctgg acaaggcaag
    17341 gcctgttatt gccaaaaccc atgaagtatt tctaatggaa gggtttatgg acgtgattgc
    17401 cgcttaccgt tccggctatg aaaatgccgt tgcttcaatg gggacggcat tgactcaaga
    17461 acatgtcaat caccttaagc aagtcactaa aaaagttgtt ttgatttatg atggtgacga
    17521 tgctggacaa catgctattg caaaatcact agaattgctt aaagattttg ttgtcgaaat
    17581 tgtcagaatg cccaataaaa tggatcctga cgaatttgta caacggcatt ccccagaagc
    17641 atttgcagat ttgcttaagc agtcacggat cagtagtgtt gaatttttta ttgattacct
    17701 aaaacctact aatgtagaca atttgcaatc acaaattgtt tatgtggaga aaatggcacc
    17761 attgattgct caatcaccat ccatcacagc tcaacattcg tatattaaca agattgctga
    17821 tttgttgcca aactttgact attttcaagt agaacaatca gtaaatgcat taaggattca
    17881 agataggcaa aagcgtcaag gtcaaatagc tcaagccgtc agcaatcttg tgaccttacc
    17941 aatgccaaaa agtttgacag ctattgctaa gacagaaagt catctcatgc atcggctctt
    18001 acatcatgac tatttattaa atgaatttcg acatcgtgat gatttttatt ttgatacctc
    18061 taccttagaa ttactttatc aacggctgaa gcaacaagga catattacat cttatgattt
    18121 gtcagagatg tcagaggaag ttaatcgtgc ttattacaat gttttagaag aaaaccttcc
    18181 caaagaagta gctcttggtg agattgatga tattttatcc aaacgtgcca aacttttagc
    18241 agagcgcgat cttcacaaac aagggaaaaa agttagagaa tctagtaaca aaggcgatca
    18301 tcaagcggct ctagaagtac tagaacattt tattgcgcag aaacgaaaaa tggaatagag
    18361 gaaattatga caaaacaaaa agaaataaca acttttaacg ttcaagttgc ggagtttatt
    18421 cgccatcaca aaaaagaagg aacagcgatt gatgatgacg tgaccgaaaa acttgtcatt
    18481 ccatttgctt tggatgctga tcagattgat gatttacttg agcggttaac agatggtggt
    18541 atctcgatta cggataaaga aggtaaccca tcatcaaaat acattgttga agaaccaaaa
    18601 ccagaagaat taactgatga agaattaatt ggtagtaatt cagctaaagt aaacgaccct
    18661 gtccgcatgt acctcaaaga aattggtgtg gtaccgcttt taacaagtga agaggaaaaa
    18721 gaattggctg ttgcggttgc caagggagat ttaatggcta aacaacgttt agcggaagca
    18781 aaccttcgtc ttgttgtctc aattgcaaaa cgttatgttg gacgtggaat gcaatttctt
    18841 gatttaattc aagaaggtaa catgggactc atgaaagctg ttgataaatt tgattattca
    18901 aaaggattca aattctcaac ttatgcaaca tggtggattc gtcaagctat aacacgcgca
    18961 attgcagatc aagcacgtac gattcgtatc cctgttcata tggttgaaac gattaacaaa
    19021 ttagtccgtg aacaacgtaa ccttcttcaa gaacttggtc aagatccaac gccagaacaa
    19081 atcgcagaac gtatggaaat gactccagac aaggttcgtg aaattctaaa aattgctcaa
    19141 gaacctgttt ctttagaaac accaattggg gaagaagatg atagtcactt aggtgatttt
    19201 atcgaagatg aagttattga aaatccagta gattatacga cgcgtgttgt tcttcgtgag
    19261 caattggatg aagttttaga tacgcttact gaccgtgaag agaacgttct tcgcctacgc
    19321 tttggccttg acgatggtaa aatgcgtacg ttagaggacg taggtaaagt ttttaacgtt
    19381 actcgtgagc gcattcgaca gattgaagct aaagccctta gaaaacttcg ccatcctagt
    19441 cgtagtaaac aattaagaga ctttatagag gactaaaatc gtccagtgga cgattttagg
    19501 tcgttccttt gaaataagaa aggagcgaaa atcccaagtg gcacttatca gcggtacacc
    19561 tacaaattag aaatcggacc gaatgtggtg ggcattttta aggttggact taatatctta
    19621 aacaagccca gagggcaatt tcctctaaat gggcaaagct ttagtggttt ttttacttat
    19681 ctacgacata ttcaaaaaat gaaaggaaag ccatgtctga tacaccaaaa tatacacaag
    19741 atcaggtgat agctatcaag aatagaattc ttgaagcttt agaaactgtt attgatcccg
    19801 agctaggtat tgatgttgtt aatctaggct tgatttatga aattcggttt aatgataacg
    19861 gttatacaga gattgatatg acactcacaa caatgggttg tcctttggca gacttgttga
    19921 cagattacat ccatgatgcc ctgcaagatg tgccagaggt cactaagact gaggtcaaat
    19981 tagtctggta ccctgcttgg acggttgata aaatgagccg ctatgctagg attgcgctgg
    20041 gtattcgcta atcattgata gtaaagggat gaaatattcc cttttttgat gtagaataaa
    20101 aggaaataca ggtgaaagcc tttggttaat gttagagaac actaagatac tgagaaaagt
    20161 gttctaccta tggcaaaaag gagaactgat gattttaatt acaggaagca atggtcaatt
    20221 agggacagaa cttcgatact tattagatga acgaggtgtt gattatgtag ccgttgatgt
    20281 cgcagaaatg gacatcacaa atgaagacaa agtcgaagct gtttttgcgc aggtcaagcc
    20341 aacattggtc tatcattgtg ctgcttatac ggcagttgat gcagcagaag acgaaggaaa
    20401 agccttgaat gaggctatta atgtaacagg ctcagaaaat attgccaagg cttgtggaaa
    20461 atatggggct acccttgttt acatttcaac agactacgta tttgatggca ataaaccagt
    20521 cggtcaagaa tgggtagaaa cagatcgtcc agaccctaag acagaatatg gccgtacaaa
    20581 acgtttagga gaactagctg ttgaacgata cgcagagtat ttttatatta tccgtactgc
    20641 ttgggtattt ggaaattatg gtaaaaactt tgtctttacc atggagcagt tagcggaaaa
    20701 tcactcccgt ttgactgttg tcaatgacca acacggtcgg ccaacgtgga caagaacctt
    20761 ggcagaattc atgtgttatt tgacagagaa tcaaaaagcc tttggttact atcacttgtc
    20821 aaatgatgct aaggaagaca ccacttggta tgattttgcc aaagaaatct taaaagacaa
    20881 ggctgttgaa gtggtgccag ttgattcatc agctttccca gcaaaagcca aacgtccttt
    20941 aaattcaacc atgaatcttg ataaggcaaa agcaacaggg tttgttattc caacatggca
    21001 agaagccttg aaggcatttt accaacaagg actgaaaaag taagtattct aaaaagagtt
    21061 ggccttctaa ggccaactct ttttcatggg tttaaggtcg cattagcctt ctaattgtgg
    21121 tataatatca ctgattatat tttagaatgg ggtagtatgc aatgcaggat gtttttatca
    21181 ttggtagcag agggttacca gcgaaatacg gtggctttga aacctttgtc gaagagttga
    21241 ttagtcatca atcatctaaa aacattcgtt atcacgttgc ttgtttaagc gatacgaagc
    21301 ataaggttca ttttgattat aagggggcag attgctttta ccttaatccc ccaaaacttg
    21361 gtcctgctag agttattgct tatgatatga tggcaattac ctatgccctg tcttatagcg
    21421 accaacacca gattcagaac cctatttttt atgtgttggg caatactgta ggcgctttta
    21481 ttgcgccatt tgtgaagcag attcacaatc gaggtggacg cttttttatc aatccagacg
    21541 ggttagagtg gaaacggtct aaatggtcaa aaccagtgca ggcctacctg aagttttccg
    21601 aaaagcaaat gactagacag gctgatttgg tcatttcaga taatattggc attgaccgct
    21661 accttaaaca ggtttatcct tggtctaaaa catgttttat tgcttatgga acgcagacac
    21721 aaccttcacg tttgacaaca gcagatagca aggtcagagc ttattttcaa acctttgata
    21781 ttagagaaaa ggattactat ctcattttag gacgctttgt tcctgaaaat aattacgaag
    21841 ctgctattaa agaatttatg gcttcctcaa ctaagcgtga tttagtgatt atttgtaatc
    21901 atgaaggcaa tgcttacttt aagcaattac ttgctgagac agagtgtgat aaagacccac
    21961 gcattaaatt tgttggtacc ctttatgata aagaactatt agcctacatt cgagaacagg
    22021 cttatgccta tatccatggt catgaggtag gagggactaa tccaggcctt ctagaagcac
    22081 tagcccatac caatcttaat ttggttttag gagttgattt taaccagtcg gtggctaagt
    22141 ctgctgcgct gtattggtcc aagcaaaaag ggcaattagc agagctgatt aaccaagttg
    22201 atgcaggttt tgatagtgat catctgggca aagaagccaa agctattatt caagaacatt
    22261 acacttggga aaaaattgta ggggagtacg aggctttatt tttaaatgaa cattaatatt
    22321 ttactatcca cctacaatgg agaacgcttc ttagcagaac aaattcaaag tatccaaagg
    22381 caaacagtta acgactggac cttattgatt agagatgatg gttcaactga tggcacccag
    22441 gatattattc gcacatttgt caaggaagac aaacgaattc agtggatcaa tgaggggcag
    22501 acagaaaatc tgggagttat taagaatttt tacaccctct taaagcacca aaaagcagat
    22561 gtctattttt tcagtgacca agatgatata tggttagaca ataaactgga agtgacctta
    22621 ttggaggctc aaaaacatga aatggcagct cctttattgg tttatacaga cctcaaagtg
    22681 gtaacccaac atttagctat ttgccatgac agtatgatta agacccaatc tggtcatgca
    22741 aacacaagcc tacttcaaga attaaccgaa aataccgtaa caggcggcac aatgatgatt
    22801 actcatgctc tggctgagga atggaccact tgtgacggtt tattgatgca tgactggtat
    22861 ttagctttac tagcttctgc tactgggaaa ttagtttacc ttgatattcc aacagaatta
    22921 taccgtcagc acgatgctaa cgtgttggga gctagaactt ggtctaaacg catgaaaaat
    22981 tggttaaccc cccatcattt agtcaacaaa tattggtggt tgattacttc aagccaaaaa
    23041 caggctcaat tactattaga tttaccatta aaaccaaatg atcatgaatt ggtaacggcc
    23101 tatgtttctt tattggatat gccttttacc aaacgcttag ctaccttgaa aaggtacggt
    23161 tttagaaaaa atcgcatctt tcacaccttt atttttcgta gtttagttgt taccttattt
    23221 ggttacagga gaaagtaaga atgaattttt taacaaagaa aaaccgtatt ttactgagag
    23281 aaatggtaaa aacggatttt aaattacgct atcaagggag cgcaattggc tatctatggt
    23341 ctatcttaaa accgcttatg atgtttacga ttatgtactt ggtatttatt cgttttcttc
    23401 gcctgggtgg aaatgtacct cattttccag tagcgctttt attggcaaat gtcatctggt
    23461 cttttttttc agaagcaact agcatgggaa tggtatctat tgtatctcgg ggagacttgt
    23521 tgcgaaaatt gaacttttct aagcacatca ttgttttttc ggcagtgtta ggagctttaa
    23581 ttaattttct tattaatttg gttgttgttt taatttttgc gttgataaat ggtgtgacta
    23641 tatcagggta tgcttatctc tctctttttc tttttataga attagttgtt ttagtgcttg
    23701 gaattgcatt gttattgtcg aatgtctttg tttattatcg tgacttagct caagtctggg
    23761 aagtactatt acaagcaggt atgtatgcca ctccaatcat ttatccgatc acttttgttt
    23821 tagatagcca ccctttggcg gcaaagttgt tgatgctaaa tccagtagca caaatgattc
    23881 aagattttcg ttatttattg attgacaggg ccaacgtaac gatttggcag atgtcaacca
    23941 attggtttta cattgttatt ccatatttag taccatttgt tatattattt attggcatct
    24001 ttgtctttaa gaaaaatgcc gatagatttg cggagattat ttaatgatta atagagaaat
    24061 tgcagtaaaa gtacagcatg ttagtaaaac atttaaactg cctacagaag caactaaaag
    24121 ttttcgcacg actctggtta atcgcctaag agggattaaa ggatttactg aacaacatgt
    24181 gctaaaagat attaactttg atgtttataa aggcgatttt tttgggattg ttgggcgtaa
    24241 cggctcggga aaatcaacgc tccttaaaat tatttcccag atctatgttc cagaaaaagg
    24301 gcaggttaca gtagatggga agatggtttc ttttatagag cttggagtgg gattcaatcc
    24361 agaattaaca ggacgagaaa acgtctatat gaacggcgcc atgctgggat ttaccaaaga
    24421 cgaagttgat gatatg
//
LOCUS       DAUCWO010000025        23915 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUCWO010000025 DAUCWO010000000
VERSION     DAUCWO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23915
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.025"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(11..349)
                     /gene="cadX"
                     /locus_tag="VGY20_000900"
     CDS             complement(11..349)
                     /gene="cadX"
                     /locus_tag="VGY20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ4273767.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLSNQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            complement(361..975)
                     /locus_tag="VGY20_000901"
     CDS             complement(361..975)
                     /locus_tag="VGY20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ4273768.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            complement(1268..1417)
                     /gene="rpmG"
                     /locus_tag="VGY20_000902"
     CDS             complement(1268..1417)
                     /gene="rpmG"
                     /locus_tag="VGY20_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4273769.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(1433..1615)
                     /gene="rpmF"
                     /locus_tag="VGY20_000903"
     CDS             complement(1433..1615)
                     /gene="rpmF"
                     /locus_tag="VGY20_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ4273770.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1835..3115
                     /locus_tag="VGY20_000904"
     CDS             1835..3115
                     /locus_tag="VGY20_000904"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ4273771.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFENISEQLLS"
     gene            3453..5201
                     /gene="aspS"
                     /locus_tag="VGY20_000905"
     CDS             3453..5201
                     /gene="aspS"
                     /locus_tag="VGY20_000905"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ4273772.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPAAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            5194..6135
                     /locus_tag="VGY20_000906"
     CDS             5194..6135
                     /locus_tag="VGY20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4273773.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDIPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIITSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            6155..7033
                     /locus_tag="VGY20_000907"
     CDS             6155..7033
                     /locus_tag="VGY20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4273774.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGITGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            7076..7948
                     /locus_tag="VGY20_000908"
     CDS             7076..7948
                     /locus_tag="VGY20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4273775.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(7975..8283)
                     /locus_tag="VGY20_000909"
     CDS             complement(7975..8283)
                     /locus_tag="VGY20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ4273776.1"
                     /translation="MPSEKEILDALSKVYSEPVIQTDDYFRQAIFELASQLEKERMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKACHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(8371..10062)
                     /locus_tag="VGY20_000910"
     CDS             complement(8371..10062)
                     /locus_tag="VGY20_000910"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ4273777.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFAKMGYQPVKINHLGD
                     WGKQFGMLIVAYKKWGEEAAVQAHPIDELLKLYVRINAEAEIDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLELLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCCATATVLKEALRLLGVDAPNEM"
     gene            10353..10790
                     /gene="argR"
                     /locus_tag="VGY20_000911"
     CDS             10353..10790
                     /gene="argR"
                     /locus_tag="VGY20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4273778.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            10787..11139
                     /locus_tag="VGY20_000912"
                     /pseudo
     CDS             10787..11139
                     /locus_tag="VGY20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            11126..13681
                     /gene="mutS"
                     /locus_tag="VGY20_000913"
     CDS             11126..13681
                     /gene="mutS"
                     /locus_tag="VGY20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ4273779.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFNSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDYYRKVMREGTGWIADIEAKERQESGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKTHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(14077..14730)
                     /locus_tag="VGY20_000914"
     CDS             complement(14077..14730)
                     /locus_tag="VGY20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273780.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKPENKTYEIPCSILE
                     KQDNRNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEELFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(14895..15278)
                     /locus_tag="VGY20_000915"
     CDS             complement(14895..15278)
                     /locus_tag="VGY20_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ4273781.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKLDYRKILIECYLISEKKPQQDIIAELNRSQSWYY
                     ETKKRALLEFVEFYRDGVLKNNVRL"
     gene            complement(15253..15615)
                     /locus_tag="VGY20_000916"
     CDS             complement(15253..15615)
                     /locus_tag="VGY20_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ4273782.1"
                     /translation="MSAKEQLKELKPLFALITLFEEQRDKDIKLINAFHNPEAIRHIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEILDKLGYSRNTVF
                     KLHREALEVLDEQEERYS"
     gene            complement(16018..16506)
                     /locus_tag="VGY20_000917"
     CDS             complement(16018..16506)
                     /locus_tag="VGY20_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273783.1"
                     /translation="MLELSIENIIKPMKIQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPYCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            complement(16580..17089)
                     /locus_tag="VGY20_000918"
     CDS             complement(16580..17089)
                     /locus_tag="VGY20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273784.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYAEDRKLSKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHHSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            complement(17091..17264)
                     /locus_tag="VGY20_000919"
     CDS             complement(17091..17264)
                     /locus_tag="VGY20_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273785.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYDELTKYLLSGQQPNWVACPSAI
                     INLSDIIAITKEK"
     gene            complement(17270..17443)
                     /locus_tag="VGY20_000920"
     CDS             complement(17270..17443)
                     /locus_tag="VGY20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEQ4273786.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            complement(17440..17694)
                     /locus_tag="VGY20_000921"
     CDS             complement(17440..17694)
                     /locus_tag="VGY20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273787.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNTKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            complement(17988..19381)
                     /locus_tag="VGY20_000922"
                     /pseudo
     CDS             complement(17988..19381)
                     /locus_tag="VGY20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
     gene            complement(19393..20259)
                     /locus_tag="VGY20_000923"
     CDS             complement(19393..20259)
                     /locus_tag="VGY20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEQ4273788.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYIAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPITTGDPEDYQRYVNRGLGYPVPKNGNTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            complement(20260..20532)
                     /locus_tag="VGY20_000924"
     CDS             complement(20260..20532)
                     /locus_tag="VGY20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273789.1"
                     /translation="MNVFSQDYESKLLEQNLTAFNRFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVSV"
     gene            complement(20535..20843)
                     /locus_tag="VGY20_000925"
     CDS             complement(20535..20843)
                     /locus_tag="VGY20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273790.1"
                     /translation="MTLPENYRRVLNLIKVGAEISLILKLEERSVQSIISSLITRYNV
                     PIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSYKELLKGG
                     "
     gene            complement(20840..21202)
                     /locus_tag="VGY20_000926"
     CDS             complement(20840..21202)
                     /locus_tag="VGY20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273791.1"
                     /translation="MTTEEYFKKLLEDSEKQPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRNYNKADAVVKFLESIPIEMEYKKGLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            complement(21214..21405)
                     /locus_tag="VGY20_000927"
     CDS             complement(21214..21405)
                     /locus_tag="VGY20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273792.1"
                     /translation="MQELNLTPTQTLILFFVLSLLGLLLSLSKSLIDIDLPEDTQVPK
                     PPKNANYGAYIQSQNHYYN"
     gene            complement(21405..21737)
                     /locus_tag="VGY20_000928"
     CDS             complement(21405..21737)
                     /locus_tag="VGY20_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273793.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKNKAEAYFTRLYNQH
                     IAWVIIANVMTEYVIKFRKSATSFEEAWDALDYQRTTEIVFRAVNGLPCSEKDSGELE
                     TYLSEVSA"
     gene            complement(21994..22236)
                     /locus_tag="VGY20_000929"
     CDS             complement(21994..22236)
                     /locus_tag="VGY20_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEQ4273794.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPNADREQFEAITQE
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGNY"
     gene            complement(22263..22880)
                     /locus_tag="VGY20_000930"
     CDS             complement(22263..22880)
                     /locus_tag="VGY20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEQ4273795.1"
                     /translation="MNKTETWNGYTIRFVEHQGEWWAVAKDITNALGLKQPSRAISTL
                     KGVTKSKTLTKGGEQELSIINEKDIYRLVFKSRKPEAEAFQEWVFETIKQLRQSTGLE
                     GFQVFRMFDKEHQKQSMNRLVNGLQNATKKDLIKANTIANKAVSNKYGLPKMISKNEM
                     TENMLRDREPILDETVELIKVKEKYGLNFSVSEAIFNQNTIKKAQ"
     gene            complement(22890..23657)
                     /locus_tag="VGY20_000931"
     CDS             complement(22890..23657)
                     /locus_tag="VGY20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEQ4273796.1"
                     /translation="MRRNYSKVIDELRTSYNLNLVAIGQRIGIDPRTVGKWWQGKHNP
                     NQESRKKLNRLYREVKETMMTQVNIFEEANDNTKQVMQVITTTNFHGQPLDIYGDIQE
                     PLFLARAVAEMIDYTKTSQGYYDVQAMLRKVDEDEKLKGMALEGTTKNFRSGQKVWFL
                     TEHGLYEVLMRSNKPKAKEFRKAVKNILKEIRLNGYYMQGELVQELAQSSTQKLPGIS
                     DLTYIRNKLADLVDMDNLADISNGIDRVQQLVKLISL"
     gene            complement(23691..23897)
                     /locus_tag="VGY20_000932"
     CDS             complement(23691..23897)
                     /locus_tag="VGY20_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4273797.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
BASE COUNT         6808 a         4371 c         4767 g         7969 t
ORIGIN      
        1 aaaatagttg ttatcctagt tgattaaaaa aatctctgat ttcctcatct tttataaaat
       61 aatatataat tttcccctct cttctagtgt ccaagatgtt ttgattggat agtttacgaa
      121 gatggtggga ggcagatgcc atactgagat ttaataaaca ggctatatcg cagacacaga
      181 gttcttcgac ggcaaggaga taaaagatga tatttatctg tttattatcg gtaaattttg
      241 ataaaatacg aagtgatttt tggacttttt ccttttcaag gtagttcgtt gcggttgtaa
      301 cattttgttg atttataaca cccacttgac agatactatc ttttttcata attttttctc
      361 ctagcctaac acagcccata gcatgtcaaa gctgttgttt tcaatcagga tatacatccc
      421 caatcctaaa tagacaacgg caataaacca tctgctatat ttttccaaag tttctccaac
      481 agaagggact tgtgccaatt tttgggcaga aaaaaccaag agataaatca tgactagaaa
      541 ggtaagtaaa gccactatca aattcgctaa atttaaggta gtaaaatatg ggacaaagac
      601 accaatattg tcagcaccac aacttgcaaa agtaatcata gcgactagaa aaatcaggtt
      661 tttattatct ttgctcaaac cttctttggc aatagcttct ccatcagaat ctcctaaaag
      721 caaaactttg aggcctagga aaattggaat caaaccgagc aaacctaaaa tctctttact
      781 aggaatataa tctaagacaa atgcaaaaag caaacttagc aatattagac taacagagcc
      841 tagaaattgt cctaaataga tgttaatgat gtcttttctg ctttttcttt tggcaaaaaa
      901 taacattagg ataataagta agtctacggc tgtcccagaa tacaggatta ttgaagtaac
      961 aacattttga atcataaaac acctcattca aatatatttt tgaatgaatt ttaacattaa
     1021 actttgtaga tgtcaatttc agctacacca aaatatagat aagaagttag tgtaccaaat
     1081 ataaaaaagc cctgccatcc aaatgataac ggggcttaac ttcaatatcc aaatgatata
     1141 ttcatctaaa aaaggtagag taaaaggtag agttcttatt gatttatatt acaatgtaat
     1201 gaacttcaaa aattcaaaaa tcgcttaaaa tcaacgtttt gacgtctaat gacgtgtagt
     1261 gaacccctta cttaacctct gtaaatgtaa cgtgtttacg taattttggt gagtattttt
     1321 tcaattgaag acggtctgga gtgttacgtt tgttttttga agtaaggtac aagcgttcac
     1381 cagattcttt atgttcaagt gtaatattta cgcgcatggt atctcccttc tattatttag
     1441 cttcgttagc tttagcgatt ttacgtcctt tgtagtatcc tttaagggat acacggtgag
     1501 aacgtgagta atctccagta gtttcgtcga attgtacaga tggagctgtc aatttgtagt
     1561 gtgtacgacg tttgtttttc ttcgcttttg acgtgtgacg tgcaggtact gccattttct
     1621 tgttcttcct ttcgatgtgt aagttttgat tttcatcaac ttcactatga taacatatct
     1681 tttttgtaaa gtaaagttac ttgacaaaac tttttatttt tacaatttca tggtgaaaat
     1741 actatcaaac taaagcaaat gatatatgct tgtttaaaat ataagaaaaa atggtagaat
     1801 gtttaaagag aaaactaatc aaagaggtaa acttatgaaa cttcaaaaac caaaaggaac
     1861 ccaggatatt ctgcctgggg atgctgcgaa atggcagtat gtggaatctg ttgcgcgtga
     1921 tacgtttagc caatataatt atggagaaat tcgtactcct atgtttgaac actacgaggt
     1981 aatctcgcgg tctgtaggtg atacgactga tattgtgacg aaggaaatgt atgatttcta
     2041 tgataaagga gatcgtcaca ttaccttgcg accagagggt acagctccag tggtgcgttc
     2101 ttatgttgaa aataaattgt ttgcgccaga ggtccaaaaa cccgtcaaac tttattatat
     2161 cggttccatg ttccgctacg aacgtcctca agctggtcgt ttacgtgaat ttcaccagat
     2221 tggggtagaa tgtttcggcg ctgctaatcc agcaacagat gttgaaacca ttgcgatggc
     2281 ctatcacctc tttgaaaaat tgggcattaa ggatgtcacc cttcatttaa atagtttggg
     2341 gagtcctgag agtcgtgcag cttatcgtca agccttgatt gactacttaa caccaatgcg
     2401 tgatcaatta tctaaagata gtcaacgtcg cttggatgaa aacccactgc gcgtgcttga
     2461 ttctaaagaa aaagaagata aactagccgt tgaaaaggcg ccttctattt tggattactt
     2521 agatgaggaa agtcaggcgc actttgaggc ggtcaaagat atgcttgaag cacttgacat
     2581 tccttatgtg attgacacca atatggtgcg tggccttgat tactataacc acactatttt
     2641 tgaattcatc acgtctgtgg aaggttctga cttaactatc tgtgcgggtg gacgttatga
     2701 tagtttggta ggttattttg gtgggcctga gactccagga tttggctttg gtcttggttt
     2761 agaacgtctc ttgatgatta ttgaaaagca gggcattacc ttgccaattg agacagagat
     2821 ggatatttac cttgctgtgt taggtgatgg tgctaatagc aaagctttag aattggttca
     2881 agccattcgc cgtcaagggt ttacagctga acgagattac cttggccgta agattaaggc
     2941 tcagtttaaa tcagctgata cctttaaagc aaaattggta atgactttag gagaaagtga
     3001 agttgaagca ggcaaagctg tgattaagaa taatcgtagc cgtcaagaag tggaagtcag
     3061 ttttgaagat atgatgacca actttgaaaa catctcagag caattattgt cttaatgatt
     3121 ttattgtcgt cataagcttg tgttgatatg attgtttaga agataatcac taaaacaggt
     3181 gtaacttctc tttttagaag ggagttgcac ctgtttgttt tctgtaaggg gattgattag
     3241 ctttcttagt attctgtgag gacacctgat atgtcaaaat gctgacgact cagtattctt
     3301 gctttatctt tagaaaaact ctttttaaat gggtggggtt tttaagggct ttttgctata
     3361 atagtaggga atgaattaat tgatttatat agtgaaagga attggaaacg agcaggtttc
     3421 acaggattct gtttgtttta gggaatttat ttatgaaacg tagcatgtat gcagggcgtg
     3481 ttcgcgaaga acacattgga acgacaatca cattgaaagg atgggtcagt cgtcgtcgtg
     3541 acttgggtgg tctcatcttt attgatttgc gtgaccgtga gggtgtgatg caattagtca
     3601 ttaaccctga ggaagtttcc agtgatgtga tggcaactgc tgaacgctta cgtagtgaat
     3661 atgtgattga agtggaaggt tttgttgaag ctcgacaaca agccaatgat aagttggcga
     3721 caggaatggt tgagttaaaa gtatctgctt tgaccatttt aaacacagct aaaacgacgc
     3781 cttttgaaat taaagatgac gttgaagtaa gtgacgacac gcgtttacgt taccgttact
     3841 tagatttacg acgtccagaa atgttagaaa acttcaagct acgtgcaaaa gtgactcatt
     3901 ctatccgtaa ttaccttgat gaccttgagt ttatcgatgt cgagacacct atgttgacaa
     3961 aatcaactcc agaaggtgcg cgtgactatc tggtaccaag tcgtgttagc caagggcatt
     4021 tctatgcttt gccacaaagt ccgcaaataa ccaaacagtt attgatgaat gctggctttg
     4081 atcgctatta tcaaattgtg aaatgtttcc gcgatgaaga tctacgtggt gaccgtcaac
     4141 ctgagtttac acaagttgac ttggaaacgt cattcctttc tgaacaagaa attcaagaca
     4201 tcgttgaagg catgattgct aaagtgatga aagagacaaa agaaatagat gtaaccctac
     4261 cattcccacg tatgagctac gatgtagcaa tgaatagcta cggctctgat aaaccagaca
     4321 cacgttttga aatgctttta caagacttga cagtaactgt caaaggaatt gacttcaagg
     4381 ttttctcaga agcaccagct gtcaaggcga ttgtggtgaa gggaaatgca gatcgatact
     4441 ctcgtaaaga tattgacaaa ttaactgagt ttgccaaaca atttggtgct aaaggccttg
     4501 cctgggttaa ggtgacagat ggtcaattgg caggcccagc agccaagttc ttgacagcta
     4561 ttgagacaga gttgtcaagc caattaaaac ttgctgagaa tgatttggtt ttatttgtag
     4621 cggatactct tgaggttgct aataatacat taggtgcgct tcgtaatcgt attgccaagg
     4681 accttgacat gattgatcag tcgcagttta atttcctttg ggtggttgat tggccaatgt
     4741 ttgagtggtc agaagaagaa ggacgttata tgtcagctca tcaccccttc actcttccaa
     4801 cgccagaatc tgctcatgag ttagagggtg atttggctaa ggtacgggct attgcttacg
     4861 acatcgtttt aaatggttac gagcttggtg gtggtagcct gcgtatcaat caaaaagaga
     4921 tgcaagagcg catgttcaag gctcttggct ttaccgctga tgaagctaac gaccaatttg
     4981 gtttcttact agaagcgatg gactacggct ttcctcctca tggtggcttg gcaattggtc
     5041 ttgaccgttt tgtcatgttg cttgctggaa aagacaatat tcgagaagtt attgccttcc
     5101 ctaaaaataa caaagcctct gatccaatga cgcaagcacc tagtcttgtt tctgaaaacc
     5161 aattagaaga actcagtctt caaatagaaa gtcatgatta agaaaacaac ctataagaaa
     5221 aaagttaaat atgtcattag tagaggggcc aagaaagttg gcctactcca cgctctaaga
     5281 agtatttcaa gagaaaaata tgcagagaag atttcggctt ctctgcttta tggcattctc
     5341 tctagtattg ctgtgaattt tttcttccag cctgggcatg tttattcaag tggagcaact
     5401 ggtctagcac aggttttttc agctcttagt catcgtcttt taggctatga tattcccatc
     5461 gcctttgcgt tttatttgat taatattcct ttgcttattt tagcttggta taaaattggg
     5521 catcaattta ccatttttac ctttatcaca gtcagcatga gttctttctt tattcaaatc
     5581 atgcctcaag tgacgctgac gactgatcct cttatcaatg ctatttttgg tggcttggtt
     5641 atgggaatgg gaattggtac aggtctcaaa tcacgtatct ctagtggggg gactgatatt
     5701 gtcagtttga cccttagaaa acgaacaggc aaggatgtgg gcagtctctc attgatggtt
     5761 aatggtgcaa ttttagcctt tgcagggatt ttatttggct ggcagtacgc cctttattct
     5821 atggtctcta tctttgtatc aagtcgtgtt acggatgcca tttttaccaa gcaaaagaaa
     5881 atgcaggcaa ccattattac cagccatcca gagcgtgtga ttcatatgat ccataaacgt
     5941 ctgcatcgcg gggtgaccag tatcaacgac gcagaaggga cttacaagca tgaacaaaaa
     6001 gcagttttga tcaccatttt gacatgtgaa gaatacccag aattcaaatg gctgatgtta
     6061 aaaacagacc cacaagcctt tgtttcagtg gctgagaatg ttagaattat cggtcgtttt
     6121 gtggaagatg attaagaaaa acacaggagg tgtcatgtca attgaccaac gtttacagtt
     6181 aaagaaattt tgctttatta ttttaggagc tgctatctac gcctttgctt ttgtttattt
     6241 ttacatggct aatcgcattg cagccaatgg tttagctggt ctcaccctag ttggtaaggc
     6301 actttttgag attgatcctt ctttgatagg ctatttgatt aatttgcctt tggtgttgtt
     6361 gggagctaga tgttttggga aaagagccat ggtttacaca gtaacgggta ttttgtcact
     6421 ttactttttt gtgtggctct ttcaacgctt tcctttggtc gttgacttgg atcatgacaa
     6481 tttagtcgtt tccttgatgg ctggaattac tggtggtcta ggtggcggtc ttgtgtttcg
     6541 caatggtgga accatcggtg gagcagatat tattgctaag ttattagaag acaagttagg
     6601 actacagctg aatcaagctc ttttaggcat tgatcttttt gtcatgattg tctctttgac
     6661 ttatatttcc ctgccgcaaa tgatgtatgc tctgattgct agctttatgt atggtcaaat
     6721 tgttcgcttg gtacaacaag gtggttattc agcccgtggc atttttatcg tttcagaaca
     6781 agcggagaaa attgcccggt tcatcatgga cgaattggga aggggagtta cttatctaac
     6841 aggtgaagga gcctactctg gtcgccgtaa aaaagtgatt tatgtggctt tggggcgaag
     6901 agatattagg gaattaaaag cttttttaac tcaggttgat cccaatgcct ttatcactta
     6961 ttttgatgtt aatgaagtca atagcccaga atttttaact attaaaagca aataccataa
     7021 aaagagaaaa taaatgagca ttagagttgc ctgcgaagaa tagtgaggat aaaggatggc
     7081 acatcacgat aaattgacta aactactaaa actgtttttg attgccctag gtgtagccat
     7141 ttataccttc ggttttgtta attttaacat ggctaatgct ttggctgaag gtggtgtggc
     7201 aggaataacc ttgattttac acgcgcattt tgggattaat cctgcttact cttctctctt
     7261 atttaatctt ccacttttta ttttaggggc aaagattttt gggaagcgtt ctttggctct
     7321 aaccatctat ggaacagttc tgatgtccgc ttttatctgg atgtggcaaa aagttcctat
     7381 cgaacttggc ttggaaaatg acatgatgtt ggtggctgta gtggcaggtc tcttttcagg
     7441 gattgggagt ggcattgttt ttcgctatgg tgcaaccaca ggtggaacgg atattattgg
     7501 tcgcattgca gaagaaaaat ttggagctaa gctaggtcaa actttgcttt tggtggatgc
     7561 tcttgtattg acagcttcgt tgacttatgt ggatttaaaa cacatgcttt atactttggt
     7621 ggcaagcttt gtttttagcc aaatgattag tgtggttcaa aatggtggtt acactatccg
     7681 tggaatgatt attattacca agcattcaga ggctgccgct caagctatcc taaccgaaat
     7741 caatcgtgga gtgacttact tgaaaggtca aggagcttac tctggtaatg attacaatat
     7801 catgtatgtg accttgaacc caactgaagt tcgggaagtc aaacgtattt tagctggttt
     7861 ggatccagat gcctttatct ccattattga tgtggacgaa gttattagct ctgattttaa
     7921 aattcgccga agaaattatg ataaataaaa atacaggtca aacagcctgt atttttattt
     7981 tggtccccca cttaagaaac taccaagcat aatagctgat atagcagttc ccttgtaatg
     8041 gcaagctttt gtttttacca agcgactaag atcaaatatg cttttaggag catcaaattg
     8101 atgtgttaag atgtattgat tgataagtga gtcaattttt gtagctagta gtgagctcat
     8161 cctctctttt tcaagttgac tggcgagctc gaagatcgct tgacgaaaat aatcatcggt
     8221 ttgtattacc ggttcgctat aaactttact aagagcatct aatatttctt tttctgatgg
     8281 catggcattc tcctgtaaca tattgtgcta ttattataat ataaaaaact aagactgtca
     8341 taaatgtgac agccttagta atgttattac ttacatttca tttggtgcgt caacaccgag
     8401 taagcgaagc gcttctttaa gaacagtagc agttgcacag cagagagcta agcggttatc
     8461 acgttctgag ttgtcgtcaa gaatgcgagt gtgagcataa tatttgttaa agctttgggc
     8521 taagttaata gcaaatttag ccataataga aggctcaaag ttatctgatg ttcgcttgat
     8581 aatgcgtggg aaatcttgga taagtttgat aatttcccag ctttcagcat cagctaaact
     8641 gtaagtggtg gttgcagatg gcgtaaagtc agctttacgt agaatagatt ggatacgagc
     8701 atgagcatat tgaacgtaag gtccagtttc tccttcaaag gaaaccatgg cttcaaggtc
     8761 aaagtcatac ccattcatac gatctgtttt gagatcgtaa aacttgatgg caccaacacc
     8821 aacggcatgg gcaacggctt ctttgtcagc aaggtttgga tttttcgctt caatttgaga
     8881 agctgcacgg ttaatggctt cagcaactgt aggttcaaga agaataacat tacctttacg
     8941 tgttgataat ttagcaccgc cttttgtgac aagtccaaaa gcaacgtgtg tcatgtcatc
     9001 tgaccaatcg taacccattt cttttagcac tgcttttaac tgtttgaagt gagcagcttg
     9061 ctcattacca acaacataaa cggatttagc aaagtcgtaa gtgcgtttac ggtaaagtgc
     9121 ggcagctagg tcacgggtaa tgtaaagagt agcgccatct gattttttga tcaaggcagg
     9181 gtgttcaatg ccgtattttt caaggttaac aacttgggca cctttggatt ctacaagaag
     9241 gtttttcgct tctaatagct caagaacctc atccattttg tcattataga aggcctcacc
     9301 gttgtaacta tcgaaggtga catgtaattg gtcataaagt cggttgaact caagtaaact
     9361 ttcatcacgg aaccattgcc ataattcagt agcttctttg tcgccatctt ccaatttacg
     9421 gaaccattca cgagcttctt catcgacggt tggatcaatt tcagcttcag cattgatacg
     9481 aacgtaaagt tttaagagtt catcaatagg atgtgcctga acagcagctt cctctcccca
     9541 ttttttgtag gcaacaatga gcataccaaa ttgtttaccc cagtcaccaa ggtgattgat
     9601 ttttacaggt tgataaccca ttttagcaaa gatattagca aggctatcac caataacagt
     9661 tgagcggagg tgaccgattg aaaatggttt agcaatattt gggcttgaca tgtcaatagc
     9721 aacatttcgc ccttgacctt cgtcttgctg agcatagtct gaacctgctg taatcacttg
     9781 ttcaaggacc tgtgaggaaa tcttagtttt atcaaggaaa aagttgatgt aagggccgac
     9841 agctacgact ttttcaaatt ggctctcatc aatctgttca gctaactcgc tagcaatcat
     9901 ttgaggggct ttgcgaagga cttttgctaa actaaaggct ggaaaggcaa ggtctcccat
     9961 gtcagaattt tttggggttt caagcaagtt aaaaatagca tcttgttcta actcagggac
    10021 aactttagct atttcacttg cgattagagt tttagtatcc atacggcgtc tcctaaaaat
    10081 tgtttgtcac cctattctaa catatttctt taaaataaac gaggagagga ttgataaatt
    10141 atactgaaac tttcaaggaa atgaattgct tgaggttgtt atttttctga aaatgttata
    10201 aaaagagaaa tgagatttct tttcaacagt tttcaagtga aattctagtt ggatttgata
    10261 aaaaattgct gaaaatagct gggcgaagat gaaataatac actttttttg ttataatttg
    10321 agaggataat tatacaattg gggtgatata tcatgaataa aatggagcgt caacagcaga
    10381 ttaagcgaat cattcaagca gagcatattg gcacacaaga agatattaaa aatcaccttc
    10441 aaaaagaagg cattgtggtc acccaagcaa ctctatctcg ggacttaaga gaaattggcc
    10501 ttttgaaatt gagagatgaa caaggcaaac tctattatag tttgtctgaa cccgttgcaa
    10561 caccatttag tcctgaggtg cgtttttatg ttttgaaagt tgatcgagca ggttttatgc
    10621 tggttctcca taccaattta ggagaggctg atgttctagc taatttgatt gacaatgatg
    10681 ccattgaaga tattctagga accattgctg gtgcggatac cttgcttgtg atttgcagag
    10741 acgaagagat tgctaaacgc tttgaaaaag atttggctgc aggcctatga ctagctatta
    10801 agagtctttg acacaattga ttcttgctat ccatgaattc tagtatcgtt gcttataaag
    10861 aagtgaagca atagctgtct aagctccatg attttaaaaa gtaagcttat cagatgaaac
    10921 gctatcaaca agatgccctg cttttcaaaa aaaatagata aagaaaaggc tgcgacagta
    10981 tctgcaagca ggtcaaaaga actagaagat aggctcagtc atcagccatt aattgatgat
    11041 tatcgagaaa agatgcaaga tgcaagtgat gtgattcagt atatcaccaa acgtatagaa
    11101 gatcagttaa acaaggagtt aacaaatggc aaaaactaac atttctcctg gaatgcaaca
    11161 gtatctggac atcaaaaaag attatccaga tgcttttttg ctttttagga tgggtgactt
    11221 ttatgaatta ttttacgagg acgctgtcaa agcagcacaa ctcttagaaa ttggtttgac
    11281 cagtcgcaac aagaatgcgg aaaatccaat tcctatggca ggcgtgccac atcattctgc
    11341 ccaacaatac attgatgtgt taattgagtt gggttacaag gttgctgtcg cagaacaaat
    11401 ggaagaccca aagcaagctg ttggggtggt gaagcgtgag gtcgttcaag tcataactcc
    11461 tggaacggtt gtggattcag ctaagccaga tagcgccaat aactttttgg tagctgttga
    11521 ctttgatggt tgccgttatg gattggctta tatggatgta tccacaggtg aattttgcgt
    11581 gacagatttg gcggacttta cgagtgttcg tagcgaaatc caaaacctca aggcaaaaga
    11641 agtcttacta ggttttgatt tatctgaaga agaacagacg attttggtca agcagatgaa
    11701 tttgctgctt tcttatgaag aaacggtcta tgaagataaa tctttaattg acggccaatt
    11761 gacaacggta gaactgacag cggcaggaaa actcttgcaa tacgttcaca aaacacaaat
    11821 gcgagaactc agccacttgc aagccttggt tcactatgag atcaaggatt atttgcagat
    11881 gtcgtatgcc actaagtcaa gtttagattt ggtagaaaat gctaggacta ataaaaaaca
    11941 tggaagtctg tattggcttt tagatgaaac caagacagct atggggatga ggcttttgcg
    12001 ctcatggatt gatcgacctt tggtttctaa agaagctatt ttagagcgtc aagaaattat
    12061 tcaagttttt ctgaatgctt ttattgagcg aaccgattta agcaatagtt taaaaggtgt
    12121 ttacgacatc gaacgcttat ctagtcgcgt gtcttttggc aaggcaaatc cgaaagattt
    12181 acttcaattg gggcatacct tagcccaagt gccttatatc aaagctatct tagagtcttt
    12241 taacagtcct tgtgttgaca aacttgtcaa tgatattgac agtttgcctg agttggaata
    12301 cttgattaga acagccattg atccagatgc accagcaact attagtgaag gcagtattat
    12361 tcgtactggt tttgatgagc gcttggacta ttatcgtaaa gtgatgcgag aaggaacagg
    12421 ctggattgcg gatattgagg ccaaagagcg tcaagaaagt ggcattaata acctaaaaat
    12481 tgattacaat aaaaaagatg gttattattt tcacgttacg aattcaaatc ttagcttagt
    12541 tcccgagcat tttttcagaa aggcaacttt aaaaaattct gagcgttatg gaacagcaga
    12601 attggctaag attgaaggtc agatgttaga ggctagggaa gagtcatcta gtttagaata
    12661 cgatattttt atgtgtattc gagctcaagt tgaaacctat attaatcgtt tacagaaact
    12721 ggctaaaact ttggcaacgg tggatgtttt gcaaagttta gcagtagttg ctgaaaccaa
    12781 tcattatatt cggccgcagt tcaatgataa tcatgtgatt acaattcaag aaggtcgtca
    12841 cgccgttgtt gaaaaggtta tgggagtgca ggaatacatt cccaatagta tctcttttga
    12901 ccaacagacc agtattcagc tgattacagg tccaaatatg agtggtaagt cgacttatat
    12961 gagacagctg gccttaacgg ttatcatggc ccagatgggt tcatttgtgg ctgctgacca
    13021 tgttgattta cctttatttg atgcgatttt tacgcgtatt ggggctgctg atgatttgat
    13081 ttctgggcaa tcaaccttta tggtggagat gatggaagca aaccaagcaa tcaaacgcgc
    13141 aagtgacaac tctcttattc tatttgatga actgggacga ggtacggcaa cttatgatgg
    13201 tatggcttta gcccaggcaa ttattgaata tatccatgat agagttggtg ctaagaccat
    13261 atttgcaacg cattatcatg aattgacaga cttgtcaact aagttgacaa gtctagtcaa
    13321 tgttcatgta gcaacgcttg aaaaagatgg cgatgttacc ttccttcata agattgctga
    13381 gggaccggcg gataaatctt acggtattca tgtggcaaaa atagcaggac tgccaaaatc
    13441 cctattaaag agagcagacg aagttctgac ccgtttagaa acacagtcac gatctactga
    13501 gataatatca gtcccttcac aagttgagtc aagcagcgct gttagacagg ggcaattatc
    13561 cctttttggt gatgaagaga aaactcatga gattaggcaa gcactggaag ctattgatgt
    13621 catgaacatg accccgcttc aagcaatgac aaccctttac gaattgaaaa agttgttata
    13681 gtctttcagc tgaaaatgaa aaagatgcct tcaatataga aagcatcttt ttgttctgtc
    13741 aaaaacggtc cgaggccttc ggctatttat taagtgtgtt ataatagtcc ataagaatgc
    13801 gaggaaatta tgacaaacat tattggactt ggattggcag tctttacagg gtattgacag
    13861 tccttaagca ttgatatgac tgtgtttttg aaaaaatata ggttttaagt ttccttagac
    13921 ttccctcaaa agtccacaaa aaggtgaaca aaaaaagacc tttgcaggtc ctttacacaa
    13981 tgagttcagc aggcaagaac tagcgtggtt tagatactac gcttttagtt tgccctatgg
    14041 cttattatag cacgattagt gtcattgagg aaaaaactac cctctcaacc agtctttaaa
    14101 gttccaccat tggtcttgga tgctatcgct caagcgttga tgccaagttt ttttggattc
    14161 ttcttcgata gaacgcttga ataactcctc cgcatattgt tgctcttcgg catctctctc
    14221 tttttgaaga cgttcattat gtgtttttcc atcctcaaat acaagttgcc caaagtatag
    14281 attcccgtca tccgcaaaag ataaggtaac tttgtctcct tcagcaagtg gtttttccaa
    14341 ttgaactgtg tagttgcctc ctccatcagc acgattgcca cttactgtcc atttccttcc
    14401 ttcgcagttt cgattatctt gtttctccaa aatagagcaa ggaatttcat aagttttgtt
    14461 ttcgggtttt ttaattttat taccattact tgaagttaca aaaccataaa catacccata
    14521 gggatctaga ttaacattaa tggttgtatc tcctgtttta aatggtgcta cggttactcc
    14581 aggtaagtgc gattgcccta tagttgtaaa caaatcgcca ctttcattag ctctgacact
    14641 tatagcacat attgatatta acaaagttaa catcataact aatttctttt tcactatatt
    14701 ctccttaaaa ttaaatatcg aactccacaa taccagttaa tcagtcaaaa aatccattct
    14761 actttttgat attttatgtc taatttgacc caccaagtca gctttgcaaa cgataccaag
    14821 ttcgtttctt ctaatcgtta ttttctagat ggatgtgtta actgtattag agacgtatta
    14881 atcttaaacc attgtcataa acgaacatta ttttttagca ctccatccct gtaaaattcc
    14941 acaaactcaa gcaaagctcg tttcttagtc tcatagtacc aactttgact tctgttaagt
    15001 tctgctatga tgtcttgttg aggttttttc tcactgatca agtaacactc aatcagtatc
    15061 tttctgtaat ctagtttaga cagttgatta atggcatacc taatagcgtc tagttcctct
    15121 agggcgcatt ctcggcttat ttcaaggtgt tttctgcgcg tgggatggta ctctatatca
    15181 aactggtaaa gctcggtata agttaaatca aggctattag cgatacgttg ccatctatgg
    15241 aattctttca gcttacgaat agcgttcttc ttgctcatct aacacctcta aagcttctct
    15301 atgtagtttg aacacagtgt ttcttgagta acctagttta tcgagtatct cgtcccatga
    15361 taagtcatcc acgtatctag ccttaataac agctatctgt ctctcatctt ggagtgtggc
    15421 gatcatggct agtctcttat cacgttcttt agctaaatat aagagttgtt tagctgtacc
    15481 tttttcgata tgtcttattg cttcaggatt atgaaaagca tttatcagct tgatgtcttt
    15541 atctcgttgt tcctcaaata aggttattaa agcgaaaagt ggtttcaatt ctttaagttg
    15601 ttctttagcg ctcatggtct tttctcctgg ttatggtata atttatttaa gcttaaattt
    15661 aaccaagggg tgttccgtgt ggacgtcttt ttgttttgtt cattttgtaa actagatatt
    15721 ttttgtagta cttcgctttt ggcttctaaa atcgtttcta agcacttttt agagtttata
    15781 gtataaatca tcaacttagt agctagaagc gctaaaacaa tgttttattt tagtcttaga
    15841 tggttattct ttgtggcagg gttatgggct aaaatgagat gtcaaatcct ttcatatcaa
    15901 gtttttaatc ttcggtgaaa atttcaaaaa gggaattttt tgcacggaaa agggcgcgtt
    15961 cttaagtttc cgaacaatat agccccgttt aaaaatgaag ggggtagttt ccgaatatta
    16021 tagccaatac ctgtcaccct tatccatata gtaatgaaat cttctccagt gataaaggta
    16081 tttggttaat ctcatgtact ttggacgttt aggaaagtca tcacgactat aatatccatg
    16141 aatgtgtctt gcttctggat ttactttcaa acactcttta aaggctaatt gccagtagta
    16201 gcaacagtct gtcttgcttc gattgagcgt ggcttgatga accttctgac aagaaccaca
    16261 agcaaaggca tgagaagcct taaataattt ccgacaacgt ctctcacagt aaggacacac
    16321 gaaaaagtaa cgtttgccac cataagttcc tggaatcgtt tcaagcaata ggccttgacc
    16381 attgtagtga atcactagcc catctaggtc tatgcggata gcttggtcac ctattgatcc
    16441 tgtaactctt gtcttcccct gtatcttcat tggtttaatg atattttcaa tagatagttc
    16501 taacattctt tctccttaaa actcaaacaa cccaaaacta ttgataaaac gacaaaaaga
    16561 gggaaaaccc tctgaatgtc tatttaacga gtaactgacc ttcaacaacc atatcataca
    16621 aatggttaaa ggcttgactg atagactcaa ggatggcccc taagtcttct ggcgtcatct
    16681 ctttataatt catagagtgg tgttcagcca gttggttgtg atcggagatg aaggctataa
    16741 gtgtgtctcg attattaact ttcccttgag tacttttaga taaagaaacc acgcgctgat
    16801 gatcaagttc ttcatccgtc atatcctcag gttggttgta atagtccttg aaactatcac
    16861 agatacgctt gaagaccttg cttaactttc tgtcttcagc atattttaag actaactgat
    16921 tagcatgacc accttggtca tcattgtgat aagttgcgtc aatcactggt tgctcatagg
    16981 tcccagtcat atagcctaaa atagcatggc aggctacttg tgcggtatca aagtctttaa
    17041 acgtgtagtg gaatgtgaat gtctttggtg tgtctgaaaa tgttctcatg ctatttctcc
    17101 tttgtgattg ctataatgtc tgataaattg atgatggcag aaggacatgc cacccagttt
    17161 ggttgttggc cagataaaag atatttggtc aactcatcat agagggtgcg gtctccttgt
    17221 atggttatgg tattgccacc tcgtgtgtgt aattttagtt tcatatcagt tacctgtaca
    17281 agaccagtag tcctgttctt gaatccatgt cttcataatt gccataagtg gcttcttgga
    17341 atttaatgtc tatcacagat accgataggg taaagagatt gacccgatgt tcaaaatcat
    17401 ctagtgattc attgtgtttt tgataaaata gtttgatttt catgttttag ctcctttctc
    17461 aacaggtatc gcaaagccac agtcaaagac ccattggtaa ccacgtcgtg ttagttctgg
    17521 tctggtaaaa tcacaatcac ccggtctaga ctttgtgttg aaattaattt tggtgataaa
    17581 gtcataaccg cattgaccat taggaagttc caatcggtat agctggtcgt ttggcaaatc
    17641 atcataattg taacgcattt tctttacgcc atgatttgta tagttaggat tcatatgtcc
    17701 ctcttttctc tctaaaatgt gtgctttttg tttttttaaa aatcaaatag cacagcaaaa
    17761 agcacaggga taaagccagt ggtatcaagg gggttgggca ctttgtgctt ttagtagcat
    17821 taatttgagc aaaagacttt ctttttttgc acgcgcacta ttagttataa atattattat
    17881 ctataatatt aaatactact aatactacaa tagagtataa agcctataat accaaggttt
    17941 tagcttgtat cattacgtgt agtttttatg tagcattagt gtagttttta aactcgttca
    18001 tagtaaggta cgggagttcc acctttaaat agtctttttc ttgcgggatt ctctcctttt
    18061 ttccagccgt catcattatc taagtaatca cgtatttttt gagaaataag ggccttacca
    18121 ccttgcgttg gtttttggtt aaatcctaga taagcgatat ggttaggact tgtcacttgg
    18181 agtaagcaat ctgtctgtgc ggaagggtag tcactatagc tttgagcatt atctaacggc
    18241 tctcctagct gtttgaggat gtattgccgt tgttcatact gtgataagct atcccaacct
    18301 tcaacaattt gaaactcatt cagtaattga tcgataattt ctttgtcgac gtcttcaacc
    18361 ttataatctt cttgaatgtc tgctaactga ttcattaact ctttagatgg cgttagtggt
    18421 tcataattgt taaaccatac tttggcttca gcgagtacct gtaagaaata atcttcttcc
    18481 acctccatag gatgtttttt cacgtcattg ataccacatt caatagggaa aaagcgtctt
    18541 tctgttccac tatccttaag aaaagatttt ttattagctg ttcgataaag acacagtgcc
    18601 ttggatgagg agtggcttta cgttcataag gttcacgata agtatcacta tctgaggaaa
    18661 tgaagctttt aactgtttca atttctgcct ttgacatgcc ttttagctcc cctagctcaa
    18721 taatggcatt ggcttgtatc ttctgataat cactatcatt tttaccaaac ttgatttctg
    18781 aatcagtgtg gtagctaggg agtagtcgct tagtaacggt gcttttccca gttccttgtc
    18841 ttttatcaat gagaatagga acgacttcaa actttacttt acggagataa attctagcca
    18901 tgagacctgt tagccatact ttggcaattt ctctattata ggaattatca gcacagccta
    18961 atagatcaat aaagtagcgt tctcctctag ctttaccatc ccatttttga ctttcaatac
    19021 gctgtttaat ggggtgataa gtgttctttt tagctaaagc ggtaatagct acctctatat
    19081 gttctttacg aggggtaaac cgatattttt catcaatgaa tgcaatacaa aggctggtct
    19141 gctcgttcgt ccatagccct ttttctttag accaaggaac tgctttagtg atttcaatag
    19201 ttttttcaaa ttcgttgtat ttaatacctg tatagatatt ttcgtaaaat tcaaaaacct
    19261 taccgacatt gtaggggcta ctaatgacat attctttgtc tcctctacct tttcgtgtcc
    19321 tgaaagcagg ggcaaaagca ggttgagtgg cttgcgatag tttattttga tagtctttca
    19381 attcttcttt gtctatggct tgattcctct ctttcttaat tctttatcaa gtatgcttct
    19441 aaaggttgta tctatctcat caatgggtag tggcttagtt gtcacgctgt tagctatttg
    19501 taccagctca taagccgtct ctaaatcaca atccacccat ttattaaata gcaagccaac
    19561 aaatttagtt aaggccacgt tgcgcccgcc ttcgtctcca aaaccattaa acagggtatc
    19621 tatgaccctc atggtaatag aacgctgact tctagggcgt ggcgtgtaag tagtaacaac
    19681 ttgtctgttt ggcgtgttac catttttagg aacaggataa ccaagaccac ggttcacata
    19741 gcgctgatag tcctctgggt cgcctgttgt aataggtaag ccttgtaatt gtgaccaggt
    19801 aaggctagct aaatcaaacg gtagtccaat cttatcggct atctccttga ccacttgttt
    19861 ataagttgct tcagtcatca cgtcactagg cttcatgaca aggcgataac ggggcttctc
    19921 gggggtgtgt ttaatcgttg gataaataat ataactatac tcccaaagcg tctgagaaac
    19981 gattttaggt aggttgacgc ctgtttctat ctcgtcatag tcaagaaaaa tcaaatcgcg
    20041 ataaactaaa ctagcattat tgcgcttata gctaccgttt ttctcggctg tgaccttgcc
    20101 acttaggcag tagggggctt gtgttcgctt gtattcttca atatcaatat cctcaggcgg
    20161 tttcaaaggt ctaaattgag caatatagtc aaatggttct aaaggtcctt tgtaggggta
    20221 caaataagag ctaaagcctc ttgcttcata aatagccatc tacacactta cccccaaaaa
    20281 gataagaata tcactgacct tgtaataatg tttcctggtg tcttctagtg gtggttggta
    20341 tcgtcttaac ccagcatttt cccaccgttt tagggtttta cctttgatat ttaattcctc
    20401 tttgacttgt tcggccgtga tcaaccctaa aactcttggt ttaggtttct ggtaggcttc
    20461 caaaaagcga ttaaaagcgg tcaggttttg ttctaagagt ttggattcat aatcttgact
    20521 aaatacgttc atgcctaacc tcctttgagt aattccttat aactggttaa atcggcattc
    20581 aataaaacac ttaggcgttc ttgttccttt tgtacttggt tgtaaaaggc tttagcaccg
    20641 tctagcaatt cttctttgtt agctggaata aagtaaccac gattgaatcc gtgcctaatg
    20701 ccgataatag ggacgttata gcgcgtgatt aagctactga tgatactttg gacggagcgt
    20761 tcttcaagtt tcagtattaa gctaatctct gccccaacct taatcagatt aaggacacgt
    20821 ctataattct ctggtagtgt catttcgtgt cctccttatc atttggaaac aggatttggt
    20881 tatcaataac accaagcact tgatttttaa gactatcttt taactcgttc aatgcttgta
    20941 taactatatt tagccctttt ttatattcca tctctattgg tattgattcc aaaaatttaa
    21001 caacggcatc agctttgtta tagttacgtt gtgtagtatt gataaatatt tttttcgtga
    21061 ataacggaaa atcgtcccac tctgcttcaa tatcttcatc tagcattttg atatttttgt
    21121 ctaaaaccct atcactcaac cagtttgtag gctgtttttc gctatcttct agcaattttt
    21181 taaaatattc ttccgttgtc atgcagttcc tccctaattg taataatggt tctgtgattg
    21241 aatataagcc ccatagtttg cgttcttagg tggtttaggg acttgggtat cttctggtaa
    21301 atcaatgtct attaatgact tagaaaggct aagaagaagc cctaagagac ttaaaacaaa
    21361 gaatagaata agcgtctgtg ttggtgtgag gttaagttct tgcatcatgc cgatacctca
    21421 cttaaataag tttctagttc ccctgagtct ttctctgaac aaggtaaacc gttaacggct
    21481 ctaaagacaa tctctgtggt tcgttgatag tctaaagcgt cccatgcttc ttcaaagctg
    21541 gtggcacttt ttctgaattt aatgacgtac tctgtcataa cgttagcaat aattacccaa
    21601 gcgatatgtt ggttatatag tcgagtgaaa taggcttctg ctttattttt gctgagttgg
    21661 cgatttttga acatttctag ctgttcagga gtgtatctat cttttgaaaa aggatttgtt
    21721 tctactctat atttcattat gttttttctc gcttaattat tattttctgt gtagtgtttt
    21781 tattgattgc ttgtttctta tactagattc atgctagttt taaggggtag ctccctacgt
    21841 atggtcaaaa tagcttcaat atgctataat ttaagacata aaaccccttt aataatagct
    21901 tgcctgcttt attaattgag tttagttata ctaattaaag gcttggaagt ttggtcgctg
    21961 tcaaagcctt tttttgttgc tcttgattat tgattaataa ttgccttgtt caatgtcatt
    22021 caaacgcttt tgctctgctt tgcgattata gattagcact ttgtcatcaa gcatgagcga
    22081 tacgccttcc aatacgttga aaatttcctg tgtgattgct tcaaactgtt cgcgatctgc
    22141 atttggtact ttgtcagcgt aaccctgtgc tagctcagct aaatcaacac cttcatcaat
    22201 ccatttcttc aactctttgt aagttgttgc tttcataatc atttctcctt tatccacgcg
    22261 catcactgcg ctttttttat tgtgttttgg ttaaagattg cttcagatac gctaaaattt
    22321 aagccgtatt tttctttaac cttgattagt tcaaccgttt catcaaggat aggctcacgg
    22381 tctcgcaaca tgttttctgt catctcgttt ttactaatca tctttggtaa cccatactta
    22441 ttagataccg ctttattggc aatcgtgttg gctttgataa ggtctttctt agttgcattt
    22501 tgtaagccat tgacaagcct attcattgac tgcttctgat gttctttatc aaacattcta
    22561 aatacttgga agccctctag gcctgtgctt tgtcttaact gtttaatggt ttcaaatacc
    22621 cattcttgga aagcttccgc ctcaggtttt cgacttttga atactaatct gtaaatatct
    22681 ttttcattga taatagaaag ctcttgctcc ccaccttttg taagggtctt acttttagta
    22741 acccccttta gagtcgatat agctcttgac ggttgtttta atcccaaagc atttgtaata
    22801 tccttggcaa ccgcccacca ttcgccttgg tgctctacaa atcggatagt atatccgttc
    22861 catgtttctg ttttgttcat agattgctcc tacaagctga tcagcttcac tagttgctga
    22921 actcggtcaa tcccatttga aatatcagct agattatcca tatcaactaa atcagctagc
    22981 ttatttcgta tataagttag gtcacttata cctggtagtt tttgggtgct tgattgagct
    23041 agttcttgca ccaattcgcc ttgcatgtaa tagccattca agcggatttc ttttagaatg
    23101 tttttgactg cttttctaaa ctctttggct tttggtttgt ttgaacgcat aagcacttca
    23161 taaagtccat gctcagttaa aaaccaaact ttttgaccac tacgaaaatt tttcgtagta
    23221 ccttctaaag ccattccttt aagcttttca tcctcatcta cttttcttag catagcttgt
    23281 acgtcatagt acccttggct agtttttgtg taatcaatca tttcagcgac tgccctagcc
    23341 aaaaataaag gctcctgaat atcaccgtaa atgtctaaag gttgtccatg aaaatttgtc
    23401 gttgtaataa cttgcataac ctgctttgtg ttgtcattag cttcttcaaa aatatttact
    23461 tgtgtcatca tagtttcttt cacctctcta tatagcctat tcagtttctt tctgctttct
    23521 tggttcgggt tatgtttacc ctgccaccat ttaccaactg ttcgggggtc tattccgata
    23581 cgttgaccaa tagcaactaa attcaagttg taggaagttc tcagctcatc tatgaccttg
    23641 ctataattcc ttctcatttc ttgcctgcct ttctaattaa taaagtacta ttattttcgt
    23701 acttttttac ctaaaaaaag gttatcaata gtgacaccgt acaaagatga taatttttgc
    23761 aatagtccta aagaaatatc ggagctatct ttttcgtact ttgaaatagt ttggggattt
    23821 ttcccaaccg ctccagctac ttgttttaaa gtgtaaccag cgttgattcg ggcagctttt
    23881 agtgtaattt gcgtcatgtt ttcaactcct ttcta
//
LOCUS       DAUCWO010000026        23158 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUCWO010000026 DAUCWO010000000
VERSION     DAUCWO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23158
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.026"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            105..3671
                     /gene="rpoB"
                     /locus_tag="VGY20_000933"
     CDS             105..3671
                     /gene="rpoB"
                     /locus_tag="VGY20_000933"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ4273798.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            3762..7385
                     /gene="rpoC"
                     /locus_tag="VGY20_000934"
     CDS             3762..7385
                     /gene="rpoC"
                     /locus_tag="VGY20_000934"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ4273799.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDGLEINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAE"
     gene            7537..7902
                     /locus_tag="VGY20_000935"
     CDS             7537..7902
                     /locus_tag="VGY20_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ4273800.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            7995..8933
                     /gene="tadA"
                     /locus_tag="VGY20_000936"
     CDS             7995..8933
                     /gene="tadA"
                     /locus_tag="VGY20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ4273801.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            8917..9904
                     /locus_tag="VGY20_000937"
                     /pseudo
     CDS             8917..9904
                     /locus_tag="VGY20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
     gene            9906..10232
                     /locus_tag="VGY20_000938"
     CDS             9906..10232
                     /locus_tag="VGY20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ4273802.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            10207..10635
                     /locus_tag="VGY20_000939"
     CDS             10207..10635
                     /locus_tag="VGY20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ4273803.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            10640..10876
                     /locus_tag="VGY20_000940"
     CDS             10640..10876
                     /locus_tag="VGY20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ4273804.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            10869..11303
                     /locus_tag="VGY20_000941"
     CDS             10869..11303
                     /locus_tag="VGY20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ4273805.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD"
     gene            11287..11613
                     /locus_tag="VGY20_000942"
     CDS             11287..11613
                     /locus_tag="VGY20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ4273806.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            11711..12664
                     /locus_tag="VGY20_000943"
     CDS             11711..12664
                     /locus_tag="VGY20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4273807.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            12723..13919
                     /locus_tag="VGY20_000944"
     CDS             12723..13919
                     /locus_tag="VGY20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ4273808.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            14106..14414
                     /locus_tag="VGY20_000945"
     CDS             14106..14414
                     /locus_tag="VGY20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273809.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(14497..15267)
                     /locus_tag="VGY20_000946"
     CDS             complement(14497..15267)
                     /locus_tag="VGY20_000946"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ4273810.1"
                     /translation="MKIGIIGIGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLA
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(15315..16382)
                     /gene="pepA"
                     /locus_tag="VGY20_000947"
     CDS             complement(15315..16382)
                     /gene="pepA"
                     /locus_tag="VGY20_000947"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ4273811.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEEAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKCY"
     gene            16838..17131
                     /locus_tag="VGY20_000948"
     CDS             16838..17131
                     /locus_tag="VGY20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ4273812.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            17128..17445
                     /locus_tag="VGY20_000949"
     CDS             17128..17445
                     /locus_tag="VGY20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ4273813.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWDIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            17463..18089
                     /locus_tag="VGY20_000950"
     CDS             17463..18089
                     /locus_tag="VGY20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ4273814.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDLAKHWKG"
     gene            18241..18636
                     /locus_tag="VGY20_000951"
     CDS             18241..18636
                     /locus_tag="VGY20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ4273815.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(18896..19537)
                     /locus_tag="VGY20_000952"
     CDS             complement(18896..19537)
                     /locus_tag="VGY20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ4273816.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPAWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(19557..20534)
                     /gene="dusB"
                     /locus_tag="VGY20_000953"
     CDS             complement(19557..20534)
                     /gene="dusB"
                     /locus_tag="VGY20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ4273817.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELADLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(20521..21393)
                     /gene="hslO"
                     /locus_tag="VGY20_000954"
     CDS             complement(20521..21393)
                     /gene="hslO"
                     /locus_tag="VGY20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ4273818.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDSLLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEALINDKA"
     gene            complement(21540..23033)
                     /gene="rofA"
                     /locus_tag="VGY20_000955"
     CDS             complement(21540..23033)
                     /gene="rofA"
                     /locus_tag="VGY20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ4273819.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYVEQILRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVQHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
BASE COUNT         7007 a         4253 c         4981 g         6917 t
ORIGIN      
        1 taacctatat ttaaatattc taaaaattaa catttagggc aaatgataac ttagttgcga
       61 tttgctgaaa cagcaaagct taaatagaag aaggagctaa aaacttggca ggacatgaag
      121 ttcgatacgg aaaacaccgt acacgtcgta gcttttcaag aatcaaagaa gttcttgatt
      181 taccaaattt gattgaaatt caaactgact cattccaaga tttcctcgat tcaggtttga
      241 aagaagtatt tgaagatgta cttcctattt caaactttac ggatactatg gaacttgaat
      301 ttgttggtta cgaatttaaa gaacctaaat atacccttga agaagctcgt atccacgatg
      361 caagttattc tgcaccaatc tttgttacct tccgtttggt caataaagaa actggtgaga
      421 ttaaaactca agaagtcttc tttggtgatt tcccaatcat gacggaaatg ggtaccttta
      481 ttatcaatgg tggtgaacgt atcatcgttt ctcagttggt gcgttctcca ggtgtttatt
      541 tcaacgataa agttgataaa aacggtaaag ttggttacgg atcaacagtg attcctaacc
      601 gtggggcttg gttggaatta gagactgact caaaagacat tgcctacact cgtattgacc
      661 gtacccgtaa gattccattc acaaccttgg ttcgtgccct tggtttctca ggtgatgatg
      721 aaattgttga tatctttggt gaaagcgacc ttgttcgtaa taccattgaa aaagacattc
      781 acaaaaatcc aagtgattct cgtacagacg aagcccttaa agaaatttac gagcgtcttc
      841 gtccaggtga gccaaaaact gcagatagct ctcgtagtct cttgattgcg cgtttctttg
      901 atgcacgtcg ttacgatttg gctgcggttg gtcgttacaa agtgaacaaa aaacttaata
      961 tcaagactcg tcttttgaac caaatcattg ctgaaaacct tgtggatgct gaaactggcg
     1021 aaattttggt agaagctgga actgagatga ctcgtagcgt catcgaatcc atcgaagaac
     1081 accttgacgg tgatttgaac aagtttgttt acacaccaaa tgattacgct gtggttactg
     1141 aacctgttgt tcttcaaaag ttcaaggttg tgtcaccaat tgatcctgat cgtgtagtaa
     1201 ctattgttgg taatgccaat ccagatgata aggttcgtgc attgacacct gctgacattt
     1261 tggcagaaat gtcttacttc ttgaaccttg ctgaaggtct tggaaaagtt gacgatattg
     1321 accacttggg taaccgtcgt atccgtgccg ttggtgaatt gcttgccaac caattccgca
     1381 tcggtcttgc tcgtatggag cgtaacgttc gtgagcgtat gtctgttcaa gacaacgatg
     1441 tgttaacacc acaacaaatc atcaatatcc gtcctgtcac agcagctgtc aaagaattct
     1501 tcggttcgtc tcagttgtca cagttcatgg accaacacaa cccattgtca gagttgtctc
     1561 acaaacgtcg tttatctgcc ttaggacctg gtggtttgac acgtgaccgt gctggttatg
     1621 aggttcgtga cgtgcattac acgcactatg gccgtatgtg tccgattgaa acacctgaag
     1681 gaccaaacat tggattgatt aacaacttgt cttcatttgg acatcttaat aaatatggtt
     1741 tcatccaaac accttaccgt aaggttgacc gtgcgactgg tagggtaact aacgaaattg
     1801 tttggttgac tgccgacgaa gaagacgaat acacagttgc acaggccaat tcgaaactaa
     1861 acgaagatgg cacttttgct gaagaaatcg ttatgggtcg tcaccaaggt aataaccaag
     1921 aattttctgc aagtgttgtt gatttcgttg acgtttcccc taaacaggta gttgctgttg
     1981 cgacggcatg tattcctttc ttggaaaacg atgactccaa ccgtgccctc atgggtgcca
     2041 acatgcaacg tcaggctgtg ccattgattg atccaaaagc accatatgtt ggtactggta
     2101 tggaatatca agctgcccat gactcaggcg ctgcggtgat tgctcagcac aatggtaaag
     2161 ttgtcttttc tgatgctgaa aaagtggaaa tccgtcgtca agatggctca cttgatgttt
     2221 accacattac caaattccgt cgttcaaact caggaacagc ctataaccaa cgcacccttg
     2281 ttaaagtagg agacatcgtt gaaaaaggtg acttcatcgc tgatggacca tctatggaaa
     2341 acggtgaaat ggctcttgga caaaacccag tcgttgctta catgacttgg gaaggttaca
     2401 acttcgagga cgctgttatc atgtctgaac gccttgtgaa agaggatgtt tacacatctg
     2461 ttcacttgga agaattcgaa tctgaaacgc gtgatacaaa acttggccct gaagaaatca
     2521 ctcgggaaat cccaaatgtt ggtgaagaag ccctcaaaga ccttgacgaa atgggcatta
     2581 tccgtatcgg tgctgaggtg aaagaaggcg acatcttagt aggtaaagtc acacctaaag
     2641 gtgaaaaaga cctttctgct gaagaacgtt tacttcacgc catctttggg gataaatcgc
     2701 gtgaagtgcg tgatacctca cttcgtgtcc ctcacggtgg tgatggtatc gttcgtgatg
     2761 tgaaaatctt tacacgcgct aacggcgatg aattgcaatc aggtgttaat atgcttgtgc
     2821 gtgtttacat cgctcaaaaa cgtaaaatca aggtcggaga taaaatggcc ggtcgtcacg
     2881 gaaacaaggg tgtcgtttca cgtattgtac cagttgaaga catgccatac cttccagacg
     2941 gaacaccagt tgacatcatg ttgaaccctc ttggggtgcc atcacggatg aatattggtc
     3001 aggttatgga acttcacctt ggtatggctg ctcgtaatct tggcattcac attgcaacac
     3061 ctgtctttga cggggcttca tcagaagacc tttgggacac tgttcgcgaa gctggtatgg
     3121 atagcgatgc taagacggtc ctttatgatg gtcgcaccgg tgaaccattt gacaaccgtg
     3181 tatccgttgg tgtcatgtat atgatcaaac ttcaccacat ggttgatgat aaacttcatg
     3241 cccgttctgt aggaccatac tcacttgtta cccaacagcc acttggtggt aaagctcaat
     3301 ttggtggaca acgttttggt gagatggagg tttgggccct tgaagcttat ggtgcatcaa
     3361 atgttcttca agaaatcttg acctacaagt cagatgacgt cacaggacgt ttgaaagcct
     3421 atgaagcgat tactaaaggt aaaccaattc caaaaccagg tgtaccagaa tccttccgtg
     3481 ttcttgtaaa agaattgcaa tcgcttggtc ttgatatgcg tgtgcttgat gaggatgata
     3541 atgaagtgga acttcgtgat cttgatgaag gtgaagacga tgacattatg catgttgacg
     3601 atctcgagaa ggcacgtgaa aaacaagctc aagaaaccca agaagtttct gaaacaactg
     3661 acgaaaaata agcaatcaat tcttattaaa taattattta ctggtctggg gcaaaggtcc
     3721 caggaactgg taaagtcatc aaaggcagaa aggtaaaact agtggttgac gtaaatcgtt
     3781 ttaaaagtat gcaaatcaca ttagcctcac caagtaaggt ccgttcatgg tcttatggtg
     3841 aagttaaaaa acctgaaaca atcaactacc gtacattaaa accagaacgt gaaggactct
     3901 ttgacgaagt catctttggt ccaacaaaag actgggaatg tgcgtgtggt aagtacaaac
     3961 gtatccgtta taaagggatc gtttgtgacc gctgtggggt tgaggtaact cgtgctaaag
     4021 tacgtcgtga acgtatgggt cacattgagt taaaagctcc tgtatcacat atctggtatt
     4081 tcaaagggat tccatctcgt atgggattga ctcttgatat gagtcctcgt gcccttgaag
     4141 aagtgatcta ttttgcggct tatgtggtca ttgatccaaa agatacaccg cttgaaccaa
     4201 aatcattatt aacagaacgt gaataccgtg aaaaactaca agagtatggt catgggtcat
     4261 ttgtcgctaa gatgggtgct gaagccattc aagatctctt gaaacgtgtg gacttggcag
     4321 ctgaaatcgc tgaattaaaa gaagagttga aatctgcttc tggtcaaaaa cggattaaag
     4381 cagttcgtcg tttagacgtc cttgatgcct ttaacaaatc tggaaataaa ccagaatgga
     4441 tggttcttaa catcctgccg gttattccac cagatcttcg tccgatggtt caattggatg
     4501 gtggccgttt tgcggcatca gacttgaatg acttgtaccg tcgtgtgatt aaccgtaaca
     4561 accgtttggc acgtttgtta gaacttaatg cccctggcat cattgttcaa aatgaaaaac
     4621 gaatgcttca agaagccgtt gatgctttga ttgataatgg tcgtcgtggt cgtccaatca
     4681 ctggaccagg aagtcgtcca ttgaaatcat tgagccacat gcttaaaggt aaacaagggc
     4741 gtttccgtca aaacttactt ggtaaacgag tagacttctc tggacgttcc gttatcgctg
     4801 ttggtccaac ccttaaaatg tatcaatgtg gtgtcccacg tgaaatggct atcgagcttt
     4861 ttaaaccatt tgtaatgcgc gaaattgttg ccaaagaata tgctggtaac gttaaagccg
     4921 ctaaacgtat ggttgaacgt ggcgacgaac gcatctggga tatcttagaa gaagttatca
     4981 aagaacaccc agtcctactt aaccgcgcac cgacccttca cagacttggt attcaggctt
     5041 ttgaaccggt tcttattgac ggtaaggcac ttcgtcttca cccacttgtg tgtgaggcct
     5101 acaatgccga cttcgatgga gaccaaatgg ccatccacgt gccactttca gaagaagcac
     5161 aagctgaagc tcgtcttttg atgcttgctg cagagcacat cttgaaccct aaagatggta
     5221 aaccagttgt taccccatct caggatatgg ttcttggtaa ctactatctt acgatggaag
     5281 atgctggtcg cgaaggcgaa ggtatgattt tcaaggataa agacgaagct gtgatggcat
     5341 atcgtaatgg ctatgctcat cttcatagtc gtgtgggtat cgctgttgac agcatgccaa
     5401 acaaaccttg gaaagacagt caaagacata aaatcatggt gacaactgtt ggtaagattc
     5461 tctttaatga catcatgcca gaggaccttc cttacctcca agagccaaac aatgccaact
     5521 tgacagaagg aacacctgat aaatacttcc ttgaacctgg tcaagatatc caagaagtga
     5581 ttgacggctt agaaatcaat gtgccattca agaagaaaaa cctcggtaac atcattgcgg
     5641 aaaccttcaa acgtttccgt acaacagaaa catcagcctt ccttgaccgt ttgaaagacc
     5701 ttggttacta ccattcaacc cttgctggtt tgacagtggg tatcgctgac attcctgtta
     5761 ttgataataa agctgaaatc attgatgctg ctcaccatcg tgttgaagaa attaacaaag
     5821 ccttccgtcg tggtttgatg acagatgatg accgttatgt tgccgttaca acaacatggc
     5881 gtgaagctaa agaagccctt gaaaaacgtc tgattgaaac acaagatcct aagaacccaa
     5941 tcgttatgat gatggactca ggagctcgtg gtaacatctc taacttctca cagcttgcgg
     6001 gtatgcgtgg tttgatggct gctcctaacg gacgcatcat ggaacttcct atcttgtcaa
     6061 acttccgtga aggtttgagc gttttggaaa tgttcttctc aacccacggt gcacgtaaag
     6121 ggatgaccga tacggccctt aaaacagccg actcaggtta tcttactcgt cgtttggttg
     6181 acgttgccca agatgttatc attcgtgagg acgattgtgg cactgatcgt ggtcttctta
     6241 tccgtgctat tacagatggt aaagaagtta ccgaaacgct tgaagagcgt cttcaaggtc
     6301 gttacacacg taaatcagtc aaacaccctg aaactggtga agtcttgatt ggtgctgacc
     6361 aattaatcac tgaagacatg gctcgtaaga ttgttgatgc aggtgttgaa gaagtgacca
     6421 ttcgttctgt ctttacctgt gcgactcgtc atggtgtctg ccgtcactgt tatggtatca
     6481 acttggcaac tggtgatgct gttgaagtgg gtgaagcagt tggtactatt gctgcccaat
     6541 ctatcggtga gcctggtact cagcttacca tgcgtacctt ccacacgggt ggtgtagcct
     6601 caaataccga tatcacccag ggtcttcctc gtattcaaga gatctttgaa gcacgtaatc
     6661 ctaaagggga agcggtcatt actgaagtga aagggaatgt cgtcgagatt gaagaagatg
     6721 cgtcaactcg taccaagaaa gtctacgttc aaggaaaaac tggcatgggc gaatatgtcg
     6781 taccatttac agcacgtatg aaagttgaag ttggcgacga agttaatcgc ggagctgccc
     6841 ttacagaagg gtcaattcaa ccgaaacgtc tccttgaagt gcgtgatacc ttgtcagttg
     6901 aaacgtacct tcttgcagaa gtacaaaaag tttaccgtag ccaaggggta gaaatcggag
     6961 acaaacacgt tgaggtaatg gttcgccaaa tgcttcgtaa agttcgtgtc atggatccag
     7021 gtgatacaga cctccttcca ggtacactta tggatatttc tgatttcaca gatgctaaca
     7081 aagatattgt tatatctggt ggtattcctg cgacatctcg tccagttctt atgggtatta
     7141 ctaaggcttc ccttgaaacc aattccttct tatcagctgc atccttccaa gaaacaactc
     7201 gtgttcttac agatgctgct atccgtggta agaaagacca cttacttggt cttaaagaaa
     7261 atgttatcat tggtaaaatc atcccagctg gtactggtat ggcgcgctac cgtaacattg
     7321 aaccacaagc gatgaatgag attgaagtga ttgatcatac agaagtctca gcagaagcag
     7381 aataatctca tgaaagagtc cttaggggct cttttttgtc tgagtttgtt ggcagtaaaa
     7441 agcattgaca atcagtctta gtcccaatga gcagatgttt ttcaaaatac ttatgattta
     7501 gctataataa tgcataagag gaatgggtaa tgtgttatgt atcaagtaat taaaatgtat
     7561 ggtgattggg agccttggtg gttcatcgat ggatggcaag acgatattat agatgagcaa
     7621 caatttagtg actggcaaga agcgcttgat tattttaatc aagaatggca gcgcatgaaa
     7681 gctatttttc ctagttatca tagccaaaag aatttgttag ctactttttg ggaaaaagaa
     7741 gataaaagat ggtgcgagga ctgtgacgaa gatttgcagc agtttcattc tcttttactc
     7801 cttaaaaata aagatattgt accaagcaat aattatattc ctgaatttga acaacgaaat
     7861 gattcaccac aggtagctta tctttgcaag ttaaaccttt aagtgtttgt taaaaacaaa
     7921 ataagttaaa agaaaatatc acttcttatg atattttctt tttttgttta cgaataaatg
     7981 agtaaggagg aattatggta caagcattag caaaagctat tctagcaaaa gctgaacagg
     8041 ttcaggcaca agatatttat attttgccaa gagcagatca atatgatctt tttttacgaa
     8101 taggagatga aaggagatta gttgatgttt atcagagcga tcggatggct cctcttatta
     8161 gtcactttaa attcgttgca ggaatgatag ttggtgaaaa gagacgttgt caggtgggtt
     8221 catgtgatta taagcttagt aaagataagc agttatcttt gcgcttatct agcgtgggtg
     8281 attatcgcgg gcaagaaagc ttagtgattc gtctgcttca tcatcaaaat aaaagtgtac
     8341 attattggtt tgatggattg acaaaagtag ccaatcaagt tggcggtaga gggttgtatt
     8401 tatttgcagg accagttggg tctgggaaga caaccttgat gtaccagctg atttcgaatt
     8461 atcatcaaga agcacaggtt attagtatag aagatcctgt agaaattaaa aatcaccaga
     8521 ttttacaatt acaagtgaat gatgatattg gtatgactta tgacaatttg atcaaactgt
     8581 ctttacgcca tcgaccagat attttagtta ttggtgagat tcgagatagt caaacagcaa
     8641 gagccgttat tagggctagt ctaacaggtg ccatggtttt ttcaacggtt cacgctaaaa
     8701 gtatctcggg tgtttatgta agattgttag aacttggtgt aacgaaagca gaactgtcta
     8761 attgcttagc attaattgct taccaaaggt tacttaatgg aggagcattg attgactcta
     8821 ctcaaaacga atttgaatat tattcctcat cgaactggaa tcaacaaatt gatcagcttc
     8881 ttgaggcagg acatctcaat cccaagcaag ctaagcttga aaaaattatc tagtaaacat
     8941 cagcataaat tcattcaatt actagctaat cttttatcta caggatttag cttcgcagag
     9001 gttattgctt ttttaaaaag aagccagttg cttcagctag attatgttct taaaatggaa
     9061 gagtctttat taaaaggaca aggcttagca gatatgttgt cgggtttagg tttttcagat
     9121 gctattctta ctcaaattag tttagctgat agacacggta atattgaaac aacattagta
     9181 gcgattcaac attacttgaa ccaaatggca aggatcagac gaaaaactgt tgaagttatc
     9241 acctatcctc ttattctttt actttttctt tttgtgatga tgctagggtt gagacgctat
     9301 ttaatccctc aattagaaac tcaaaatcag ataacttact ttcttaacca ttttcctgcc
     9361 ttctttatag gtttttgctc aggtcttatt ttgttgttcg ggatggtatg gctacgatgg
     9421 cgatctcaaa gccgcttgaa actttatagt cggctaagcc gttatccttt tttaggtaaa
     9481 ctgttaaaac aatacttaac tagttattat gctagagaat ggggtacgtt aataggtcaa
     9541 ggtcttgatt tgatgactat tttagacatt atggctattg agaaatcctc actaatgaaa
     9601 gaattagctg aggacatcag aatgagtctg cttgaagggc aagcctttca cattaaagta
     9661 gctacctacc ctttttttaa aaaagaatta agtttaatga ttgaatacgg tgaaatcaaa
     9721 tcgaaactgg ggggctgagt tggaaatcta tgcccaggaa agctgggaac aattttttag
     9781 tcagctttat caagtgacac aacttattca accagctatt tttttagtag ttgctgtgac
     9841 aatcgtcatg atttatgcgg caatcctatt accaatttac caaaatatgg gaggtatttt
     9901 ttaagatgat taatcaatgg aacaacttac gacacaagaa gctaaaagga tttactcttc
     9961 tagaaatgtt attggtgatt cttgtcatca gtgttttgat gctattattt gtgcctaatt
    10021 taagcaagca aaaagacagg gttacagaaa caggtaatgc cgctgttgtt aaattagtgg
    10081 agaatcaagc agaactatat gaattatctc aaggctcaaa accaagtttg agccagttaa
    10141 aggcagatgg tagtatcact gagaaacaag aaaaagctta tcaagactat tatgacaaac
    10201 ataaaaatga aaaagcccgt cttagcaatt aaagctttta ctttgcttga aactttacta
    10261 agtttgtcag tgatgagttt cattatattg ggattgtctg taccagtgac aaaaagctat
    10321 caaaaagtag aagagcatct gttttttagc cattttgaac atttgtatcg acatcagcaa
    10381 aagttagcca tattacagca aaaacagcgc gtcttggata tttcttcaac aaaaattgtt
    10441 acagaaggta atagcctaac agttcctaaa tcaataacag tgaatcatcc ttatcggtta
    10501 gttattgatc agatgggagg caaccattcc ttagctaaaa ttatttttga catgactgat
    10561 cgacgcttta aataccaatt ttatctaggg agtgggaatt atcaaaaaac aagtcaaagc
    10621 ttacatagcc cttgaaagta tgatcgcaac aggcatcctc tttagtattg tcattctcgt
    10681 cttgagcagt ttacagcaga gtcaggctgc tctaacgtac tatcgaaagc agcaagaaaa
    10741 gcttaatcta gctttgatgg cagtacaaac tagaactaaa gagatgacat tgaatggttg
    10801 tcatattact attttacgta cggatagata tattagtatt cacgatgacg aaggagaagt
    10861 gatgaagatt gagtaaacaa ttaagtaaca taaaagcttt tacccttcta gaggcgttaa
    10921 tagccttact cgtgatatca gggtctttat tggtttatca aggtttgacc cagaccctcc
    10981 ttaaacgtag ccattatcta gcccgtcatg atcaagacaa ttggctctta ttttctcatc
    11041 aattgcgaga ggagttaagt ggagcaagat tttacaaagt agctgataat aaactatacg
    11101 ttgaaaaggg aaagaaagta ctagcttttg gccaatttaa aagtcatgat ttccgaaaat
    11161 cagctagtaa tggaaaaggg tatcaaccca tgttatttgg aatatcacgt agtcatattc
    11221 acatagggca gtcacagatt tgcattactt taaagtggaa aagtgggtta gaaaggactt
    11281 tttattatgc ctttcaagac taatctcaaa gcaggcatcc ttttgtatgc tatgtttatg
    11341 gctagtattt ttttgctggt tttacaggtt tacctttctc aagtgacagc tctacacaag
    11401 gaataccaag ctcaaactga ctatgttaaa gcccgactga tagcagaaat agtttatcaa
    11461 gatcatagat ataaagcttc taatcctgtc ttttttaaag gtggtcaagt catttgtcga
    11521 gagcgaaaag agagatggat gttaattgtt aagttagatc agcaacgaca gtatcagttt
    11581 gaatacctta aacccaaaca atatagtcaa taaatagtat tgttgtcgta aatccttaaa
    11641 atgatccaat atatagtaat taaaggtgac acttcttttc atttcaagtg taatttggta
    11701 tgataaggtt atgacttttg aaaaaattga agaagcttat cagctgctct tagagaactg
    11761 tcaactaatt gaaaatgatc taaaaacaca tatatacgat gccattgttg aacaaaactc
    11821 cttttattta ggggctgagg gagctagtcc tcaagttgct caaaatagtg ataaactgaa
    11881 agccttgtgc ctgacaaaag aagaatggcg taaggcctac cagtttcttt ttattaaggc
    11941 agctcagact gagcaactcc aagccaacca tcagttcaca ccagatgcta ttggcttcat
    12001 tctgctgtat cttttggaac aattgagtga taaagatagc ttagaggtac ttgagattgg
    12061 aagtggaaca gggaacctag cccaaaccct tctcaacaac acgagcaaga gccttgatta
    12121 tgtagggatt gaacttgatg atctcttgat tgatctgtca gccagtattg ctgagataat
    12181 ggattcttca gctcatttta ttcaagaaga tgcggtaagg cctcaattac taaaagaaag
    12241 tgacattgtc atcagtgact taccagttgg ttattatcct aacgatgata ttgccaaacg
    12301 gtacaaggtg gctagttcag ataagcatac ctatgcccat catttattaa tggaacagtc
    12361 tttaaaatac ttgaaaaaag acggttttgc gatttttctg gcaccagtca atttattgac
    12421 gagccctcag agccagttat tgaaacagtg gttaaaagat tatgctcagg tggtggcctt
    12481 gattacgcta ccagattcta tttttggtca tccctcaaat gccaagtcca ttattgtctt
    12541 acaaaaacaa acagaccacc caatggaaac ctttgtctat ccaattcggg atttgaagct
    12601 tgcagagaat attcatgatt ttatggaaaa tttcaaaaaa tggaaactga gtaatgtcaa
    12661 ttaattgatt ttttgttata ataaaaaagc atcacagaaa acgatttcaa gagaggttaa
    12721 aaatgtcaaa gacaattgca attaatgcag gtagttctag tttaaaatgg caactttatc
    12781 agatgccaga agaagaagtg ttagcacaag gaattattga gcgtatcggt ctcaaggatt
    12841 ctatttcaac agttaagtac gatggcaaaa aagaagagca aattcttgat attcacgatc
    12901 atacagaagc tgttaaaatc ttattgaacg atttgattca ctttggaatc attgcagctt
    12961 atgatgaaat tacaggagtt ggacaccgtg ttgttgctgg tggtgaactt ttcaaagaat
    13021 ccgttgtggt taatgataaa gttcttgaac agattgaaga attatccgtt ttagctccac
    13081 ttcacaatcc tggagcggcg gcagggattc gtgccttccg tgatattttg ccagatatta
    13141 ccagtgtttg tgtgtttgac acgtcatttc acactagcat ggcaaagcat acttaccttt
    13201 acccaattcc tcaaaaatat tatacggact acaaggttcg taaatatggg gcgcatggga
    13261 caagtcataa atatgtagcg caagaagctg ctaaaatgct tggtcgtcca ttagaagaat
    13321 taaaactaat tactgctcat attggaaatg gtgtctcaat tacagctaat taccacggta
    13381 aatcagttga tacctcaatg ggattcactc cattagctgg tcctatgatg ggaacacgtt
    13441 ctggtgacat tgacccagct atcattcctt atttaattga gcaagaccca gaattaaaag
    13501 acgctgctga cgttgtgaat atgttaaaca aaaaatcagg tcttagtggg gtgtcaggca
    13561 tttctagtga tatgcgtgat atcgaggctg gtttacaaga agataatcca gatgctgttt
    13621 tagcttacaa tatctttatt gatcgcatta aaaaatgcat tggtcaatat ttcgctgttt
    13681 tgaatggagc agatgctttg gtctttacag ctggtatggg tgaaaatgca ccattgatgc
    13741 gtcaagatgt tattggtggc ttaacatggt tcggaatgga cattgatcct gagaaaaatg
    13801 tttttggata ccgtggtgaa atttcaactc ctgagtcaaa agtgaaggtt cttgttatct
    13861 caacagacga agaattgtgc atcgcgcgtg acgttgagcg tttgaaaaat acaaaataaa
    13921 agagactaag tctcaataga ttaaccaagt aaggcaacac tttacttggt tttttatgag
    13981 gtttatctgt tagttatggt gcctaggtat tagttctgat cagtctcctt gctttttgta
    14041 atcttgtgct aggcgatttg gatgtcagcc ttatttagaa gtttttaatt gcttcaacgc
    14101 ttttcttgta tgtgtttata gagcccaatg atctaaaaga ctatttgata acggtttatg
    14161 atgagcgtaa tcgtgcgatt cgaaaacaga cacaagttta ctgcttttac ctgatgattg
    14221 ttctatttgt tgccttgttc tttttcttcc cttgcttaac cattcgagac cttgtttctt
    14281 ataagctagc tttgattttt gcttaccttt acttgattat cagaagtctg gtggctaaga
    14341 aaatggaggc actagggaaa ataacaacta atagtcaaaa gcctccgttt gatacgagtc
    14401 aggttctttg ctagatgact tgtctaacgg ttgagtttcc tgtctggtca acggaggtgt
    14461 ttgtattgtt gtctgatttg tattggggca cttattttac aagcttttag ccttatcgat
    14521 ggttttgtca atggcagagc tgacagtagt tgtgaggcca aggcgttcta actccatcag
    14581 accagcaata gttgtgccac cggggctaca aatagcgtca atgaaatcgt gcggactttg
    14641 agaactggtc ttgagattgc tggcactagc caagactgtt tgagtaacaa tctccagcgc
    14701 ctttgcttta ggcatgccat tcttgacgcc agccttagcc aaagcctcaa taaagagata
    14761 aatataggca ggacttgacc ctgctaaagc ggtaaaggtg tcaaaatcct tttcactaat
    14821 atcaaatgtg ctaccaaagc tatctgttaa gtctcgaaca cgtgcttgta attcctggga
    14881 caccaaagca tttcccgtta aagcggtact gctttggaga atttgtgcat tcatgtttgg
    14941 catgatacgc agcagcggaa ggtcttgtcc tacgaatgtt gctagtcgtt gaagggaaat
    15001 gcctgctgcc atagatataa taggctgttt gaagtgaagc ggtttgagta ccgtttcaaa
    15061 tagttgaggc ttgatgccta aaataacaag atcaacctgg tcaataaggt cttggtggga
    15121 catagcataa ggcagtgcta actgctccgc aatttccttg gaccgttcta aagatgatcc
    15181 tgaaatgata agttcatggg gtgtttgttt aaggcctttg atgatagcgc tagccatttt
    15241 gccaatacca ataatgccaa ttttcattct gttgctcctt gtgggatatt atggttaatc
    15301 aggctaatgc tagtttagta acatttaatc aagtcaacgg ttgagcgatc cagttttttg
    15361 ataatggctt gtaagaaggc ttgggcttct acgaaatcat ccatagcgta gagggtttga
    15421 tgagagtgaa tgtagcgtgc acagactccg atggttgttg atgggacacc accattttga
    15481 aggtgtgcag cacctgcatc tgtgcctccc ttgccacaat agtattggaa attgacacca
    15541 gcttcctcag cagtagtcag taagaagtcg cgcatatctt tgagcatgac atggcctggg
    15601 tcgtagaaac gcaacaaggt gccatctccg attgttccag gattgccata aatatcacca
    15661 gcaggcgaac aatctactgc gaaaaagagt tcagggtcga acttggtggt tgagacgtgg
    15721 gctccgcgca gaccaacttc ttcttgaacg ttagcacctg caattagggt gttgttaagg
    15781 tcttgtcctt ttaacgcttc aagcatttct gttatcatga gaacaccata gcgattgtcc
    15841 caagcttttg aaataatatt tttttgattg gctgttagga tcgtttcaga ttgagggaca
    15901 ataatatcac ccggtgtaat accaaatctt tcagcttctg ccttatccgt aaagccacca
    15961 tcaaacacaa tatcttcgat atgtggtaga ctagcagagc catttgcccc acggagaaaa
    16021 tgaggaggta ccgatcctga aataagggga ataacttggc cagtgcgtgt gtaaagggta
    16081 aaccgttgtg aactgacaac aagtgggttc caaccaccga taccaaccac gcgtagcgtt
    16141 ccgtcaactt tgatatcact gaccataaaa ccgacttcgt ccatgtgcgc agctactaaa
    16201 atacgggggg ctttttcagc tttactatct ctgataccaa aaatgccacc aagcccgtct
    16261 gtttcaacac ggtcaaccag cggggttatt ttggtgcgta ggtagtcacg aacgctatgt
    16321 tcatagcccg caatgccatc cagttcggta acttctttga tttttgagaa taagtctgtc
    16381 atttttcatc tccatttaca aggtttcaga ccagttcaac gcggtcatct ccctatattc
    16441 taccactttt taagtaagaa tgggtttcac agcaaagaaa aatcccagag actagagatc
    16501 tctggaagaa gtgtttgtct tttggcgata acctgttttt ttaggcagca aggaaatcat
    16561 taaaatgata aagcacaagc tggttaataa gaatagtagg gcgcttagga tgcgaaaaga
    16621 ctcttcacta gcgaaaagta aggataatgg agtcataagg agaaccttcc attttaggtt
    16681 aatcagtgat ttttatagtg tggttaaatt aataataaac aacacctaat caatgtattt
    16741 ttagtttagt tgtttttact atttttgtca atacataaca gtagaaatct ttagttttct
    16801 tatttcagat cgtccaattt gatcttttta tgataaaatg agggatatga gtaaaaagaa
    16861 aataggtatg atttcaggta tctttggatt tagtttagct attggcctag gaatagttat
    16921 caaagattat tgccaagaca ggcagcgtcg acaaatgaca agggatttac gcaccttttt
    16981 ttcaccttta ggacaaatcg aagttttata tatcaatcct tgtcaggtca aacaagatta
    17041 tatttcaggt ggggttgtta tgtcaaacgg caaacagtac caattcacct atcacagtcg
    17101 acaaattagt tttgaggagg acaagggatg atcagaccga cttcttatga gtcattagcg
    17161 gctcttattg aaaaagaaga taagctggtg ttatttttta cggcagactg gtgtccagac
    17221 tgtcaattta tttatcccat tatgccagaa attgaagcag aacttacaga tatgactttt
    17281 gtttgtgtta atcgtgatca gtttatagag gtagcacaaa agtgggatat ttttggtatt
    17341 cctagttttg tcgtgattga aaaaggtcag gaagtagggc gtctggttaa caaaatgaga
    17401 aagacaaaaa ctgagattat gcatttttta gctgcttatc aataagaaaa gaggaagaat
    17461 aaatgatttt tgcatacaat aaagaacaag ttggcgatgt cttgatggtt atcttacaag
    17521 acaccaaaga tatcaaacgt caagtagaac gaaaaggcaa agtagcccgt gtttttgcag
    17581 aagaaagcgg caaaaccctt gcttggaata tctttgaagc gtcaagcttg attactattg
    17641 aaggcaatgg acagattttt ttgacagacg aggaccttgc aagattaaat gcagagcttg
    17701 ctaaggaagg attttcagaa aggcttgaac cgactgtggg acctgttttt gtggttggtc
    17761 aaattgttga gatggtggct catccagata gcgaccatct taatatctgt caagtggcta
    17821 ttggtgaaga tcaaacggtt caaatcgtag cgggcgcacc aaatgctgcg cttggtttaa
    17881 aaacgattgt tgccttacca ggtgctataa tgccaaatgg tagtctgatt ttcccaggaa
    17941 aattgcgtgg tgaagaaagt tatggtatga tgtgctctcc tcgtgagtta gccttgccaa
    18001 atgcaccgca aaaacgtggc attattgaac ttgatgagtc agctgtggta ggagaagctt
    18061 ttgacctagc caaacattgg aaaggttaga aaaaccagct gaaataagac tgtatttaac
    18121 taaatgacca gaaaaaaagg attaggagcc cctagtcctt ttttcttatg ttttcaaaaa
    18181 cgtttaaaat gaaacttatt ttaccacaat ttttacgaat aataagataa ggaggtcagt
    18241 atgtataata aagtgatagc aatcggtcgt ttggtggcta aaccagaatt ggtaaaaacc
    18301 gctacggata agcatgtagc acgtctctct ttagctgtta atcgaagatt taaaaatgct
    18361 tctggagagc gagaagcgga ttttatctct gttgttgttt ggggaaagtt agcagaaact
    18421 ctggtttctt atgctagcaa aggtagtttg atgtctattg atggcgaact taggacccgc
    18481 aagtatgata aagatgggca agtgcattat gtgacagaag ttctctgcca atcatttcaa
    18541 ctgcttgaaa gtcgtgctca acgcgctatg agagaaaata atgttactaa tgatctagtt
    18601 gatttagtct tagaagaaga tactcttccc ttttgatagg ggataatgct tctcatcacg
    18661 gttcagctga ttactgaaat atatgatgag gtgatatccg agcaacaaaa gtgtgaccag
    18721 atacttctga taaaaataaa aaaacacctt acgagtatga acaaagtttc aagtgctaga
    18781 tgttggaact ttgttcatca taggaatagt agggtgtttt ttagtgtatt acttcttttt
    18841 gttgctagtg ttaaggttta cttgcttaca aacggttatc aggccactag aactcttata
    18901 ataaatggag ctctttgagt ttggtgtcaa tcatttttaa gacagtcgcc aaatcttgtg
    18961 ggttttgaac gaaatcaagg gaattgccat caattttcat ttttggagag accttgtagt
    19021 cttcgtacca ggctgggtat tcaccgtgga cttgatggta atattgctca agactaggat
    19081 tgccgtcgac ctgctcaaaa cttcggccac gtttttcaat gcgctcaagc attttgtcaa
    19141 aagacacatc aatgtagatg agaagatcag ggcgtttttt aggcatgcct tctagttctt
    19201 ctaacatgtt ggcgagaagt tcctgataga tgtcaagttc tgtcttagtg acattgccat
    19261 ttttgtagtt aagttttaaa aagagttcgt cttcaaaaat agagcggtca agaatgttgt
    19321 tatcggcttg ataagcttct ttgatggact taaaccgttt attcaaaaaa taaatttgaa
    19381 gtaaaaaggc gtacttttta gggtcttggt agtagaggtc tagcacgggg ttattatcaa
    19441 cagcttcgta aaaaacgtca gttcctaaat gctctccaag tgcagcagca agcgagctct
    19501 tgccagcccc aattgttcca gctagtacaa tcaacatgcg tctccttcta atgtcattag
    19561 cgtactgttt cgaagatggc ttcaacctca gctagagtct cagcgcgtga aacagcacca
    19621 cgaactttgg cagcacctgc tgttccgcga agatagtgtg gcgctaagcc ccgaaattcc
    19681 cgaacagcaa tggtttcacc tttgaggtta atcaggcgtt tgaggtggtc tttggcgatg
    19741 tctaattttt tggcaaaagg aagatcagcc aattcttgac cagtttcaaa gaaatgattg
    19801 atttgggtaa aaagataagg attgttcatg gctgcgcggc caatcatcac ggcatctaca
    19861 ccgatttctt caatcataaa tttggcgtct tgcaccgaac gcacgtcccc atttccaata
    19921 aagggaatct tagtgatagc cttagaaact cgggctaatg tctcatggtc gcaggttcct
    19981 gtatacattt gctcacgggt acgaccgtgc atggcaagag cagatacacc tgccgactca
    20041 gccgcaaggg cattttcaac agcaagtgaa ctatccgccc aacctgtacg cattttgaca
    20101 gtcagtggaa tatctaaaac agacgtgact tccttgacaa tgtggtaaat cttatcaggg
    20161 tcacggagcc acttggcacc agcctcgttt ttgacaactt tattaacagg gcagcccata
    20221 ttaatatcaa caatatcggc cttggtattg gtttgaatga aatcagctgc acgtttcaac
    20281 ccttctgcgt caccaccaaa taattgaatg gacatagggt gctcattttc atcaatatgg
    20341 agcatgtgaa gggtcttttc gttgttgtag agcagccctt tttcggaaat catttccata
    20401 accaccaaac cagcaccaaa ttctttagcg atggtacgaa aggcagagtt ggtcacgcca
    20461 gccatgggag caaggacagt gcggtgaggg atttccacat caccaatcct aaaggaagaa
    20521 ttaagcttta tcattgatga gggcctccaa atcactttca ttaaattggt acttggtacc
    20581 acaaaattgg caaacaatct cagcaccctt atcttcgtca atcatggcct gtaaatctgc
    20641 ttttggcaaa gtcattaggg ctgcttcaaa acgttctcta gaacagtcac actgaaaact
    20701 cagaggttct tcagaaagac gtttgtaagg ttcatctcca tagatggcct ccaaaaggga
    20761 atccacatga tttttggaag ctagtaggtg agaaatggca ggcatttctt gcaggcgttt
    20821 ttcatagcga gcaatctctt cttcagaagc tcctggtagc acctgcacca taaagccacc
    20881 agcaacctta accttatcat tttcatctaa aagcacatta agtccgattg cagatggtgt
    20941 ttgctctgat tcggttaagt agtaggcaaa atcctcacca atttctccag taataagagg
    21001 cgttgttgag gtgtaagggt tacctgtgcc ataatcaata atcgtcacaa aatgaccatt
    21061 ccccataaaa ggcccaacaa ggacttcacc tgttgcggtc tttttaatat cgacaccagt
    21121 attttgaatg taacctttca catgtccttt ggtatctgcc acagagataa tgtgtccaaa
    21181 agaagaatca ccaatcactt tgacggtgat tttactatcg cctttttgat tagccgccaa
    21241 aatctggttg gcgatcaagg tacggccaag cgcaacagta gacgatgaca gcgtgttgtg
    21301 tttttcctga gctaatgcaa ccgtctcagt gctatcaagg acataagccc taaaagcccc
    21361 tgattgtgct attgatttaa ttattttatc cataacttag attatagcat aaagtgtaaa
    21421 acttgtattg acaggattgc ttgaaaaatc aatacagata gacagtaaaa aatcccagag
    21481 aatgactcgg gattaagtta aggtgtggtc taaagtcgcg caatgtggtg acatggcgct
    21541 tatgttaatt gtttggttaa atcagcttgg aatttttcct ctttaacttg atacatcaat
    21601 tcttggatag acagaatcga ttcatcaaaa gatatttcag caacagcaat tccttttgta
    21661 agctcatgct gaacaaaagg aatcagttgg ctgtgagtga tgactaaatc tggttttaaa
    21721 tcaggaattt gataaacatt atcttgcaat agataatagg aatgaaaatc aatgctttta
    21781 tccgagaaat accttggaaa agaatccgtt aggagatgag cattgataaa attactggct
    21841 acgaaaacaa caactaaagg aggttggata tttcttagaa tttgctcgac atagtggcaa
    21901 aaaagatgaa aatgcttatg gttcaagtca cgcttaccag gaagtttggc catccattct
    21961 tcgacaatta actttaagga cgtatagagt ttttggtttc ctttatagta cggagaaacg
    22021 aataagttgg tctcaggaat aaaatgttgc agattaaaca agaatgattt tgaaaaaaac
    22081 ataagagctt ttactaaact agccttttgc tcttttaggt taggtaaaag agtgatgata
    22141 ggatttaaaa gcaggcgaaa agtatcattt tcttcaaaaa gttgacaaca ttgtctgata
    22201 tgctcaggtg tccattgtaa gctcgcaaaa gaattattag cggtgatata aattaaatag
    22261 aggtagtcca aatctcccgc tgaaaagttt agttggcagt aagtttcgat aatatcatgg
    22321 ctactttttt tcaaagaatc gtagacaaaa agttttttta attgttgaaa aattctggtt
    22381 tggggaatag ttaccgaaaa ttgatgccgc ttccacgata aagccaataa aatgtcatag
    22441 aaagaaaacg attccgataa ccaaggagag gttttaaggt gggtggaact atgggataaa
    22501 aagctatgaa tagtgttttt gtcttgctgc gtcaagtcat aaactttaat gccaaactta
    22561 ctatatagca gagcgatgag gtaacggatg cgatattcct caccgacaat cttgttctta
    22621 gagagtttta attcaaagtt tcttaataaa ggaatcaatg cttcgcgcat ccgataagct
    22681 gaggagtttg ataaaaaatg acttcttgca aaatccgtaa ggggacgaga gtgggaacca
    22741 ttttttatta aaaacgctag taattgtaag acattagatg atgcatagag ttggtaaaga
    22801 taagtttctt taaaaggatg tgtaaattgg caagatatca tccttttttg gatggtcata
    22861 gacagactac cagggaaaaa ggcattcagt tcctcacaat aatggtttag ttgtaaaaag
    22921 gttaagccag ttttttctgc tacctcagtt attggcaaat aagatgtctt aaaaaacaag
    22981 ataattaact gacattttga ttcgattgat gattccaagt atttttctat caaggaaagg
    23041 gacctctctt cctaaactta gaaagccaaa taagtctttg ataagtgtat tctcctattt
    23101 caattctatc taagctaaaa atagaaatca aatctaatgg tttttggtga aaagtcga
//
LOCUS       DAUCWO010000027        21544 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUCWO010000027 DAUCWO010000000
VERSION     DAUCWO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21544
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.027"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            63..707
                     /gene="fsa"
                     /locus_tag="VGY20_000956"
     CDS             63..707
                     /gene="fsa"
                     /locus_tag="VGY20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ4273820.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            926..2911
                     /gene="tkt"
                     /locus_tag="VGY20_000957"
     CDS             926..2911
                     /gene="tkt"
                     /locus_tag="VGY20_000957"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ4273821.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNTAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLDGIIIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            3103..3369
                     /locus_tag="VGY20_000958"
     CDS             3103..3369
                     /locus_tag="VGY20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ4273822.1"
                     /translation="MEKKRQRLYDVIRHAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            3403..4137
                     /locus_tag="VGY20_000959"
     CDS             3403..4137
                     /locus_tag="VGY20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273823.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            4142..5770
                     /locus_tag="VGY20_000960"
     CDS             4142..5770
                     /locus_tag="VGY20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273824.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVVQAPFSTVALLNFWFPLLLILAMVYGIVTKVMPTYYR
                     EVFYISNENKVKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDIKWQDMTQLFTRGDGQWLTMGLIFGNLIVAGVLGFGAVIIANIIQQ
                     PLLISILLSCLILMVLGLAQLWIQKTFWKSLERL"
     gene            5835..6656
                     /gene="proB"
                     /locus_tag="VGY20_000961"
     CDS             5835..6656
                     /gene="proB"
                     /locus_tag="VGY20_000961"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ4273825.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            6649..7899
                     /locus_tag="VGY20_000962"
     CDS             6649..7899
                     /locus_tag="VGY20_000962"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ4273826.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGARLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTRRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSYHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            7919..8134
                     /locus_tag="VGY20_000963"
     CDS             7919..8134
                     /locus_tag="VGY20_000963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273827.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFVFLETVC
                     LSAGSNVVSKVKQLLIDLQVTLQNCRR"
     gene            8177..8341
                     /locus_tag="VGY20_000964"
     CDS             8177..8341
                     /locus_tag="VGY20_000964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273828.1"
                     /translation="MSWDKTFGIWLDNLLTDWCVIKEAKNLLQAVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            8365..9378
                     /gene="rsmH"
                     /locus_tag="VGY20_000965"
     CDS             8365..9378
                     /gene="rsmH"
                     /locus_tag="VGY20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ4273829.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            9383..9706
                     /gene="ftsL"
                     /locus_tag="VGY20_000966"
     CDS             9383..9706
                     /gene="ftsL"
                     /locus_tag="VGY20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ4273830.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            9710..11965
                     /locus_tag="VGY20_000967"
     CDS             9710..11965
                     /locus_tag="VGY20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4273831.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            11967..12977
                     /locus_tag="VGY20_000968"
     CDS             11967..12977
                     /locus_tag="VGY20_000968"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ4273832.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            13135..14478
                     /locus_tag="VGY20_000969"
     CDS             13135..14478
                     /locus_tag="VGY20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4273833.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHSQEEIRL
                     ANYVGGTDKLRQIEKLKGGQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            14673..15476
                     /locus_tag="VGY20_000970"
     CDS             14673..15476
                     /locus_tag="VGY20_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4273834.1"
                     /translation="MTSVFLTSGWAFYDHLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKCLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            15476..16219
                     /locus_tag="VGY20_000971"
     CDS             15476..16219
                     /locus_tag="VGY20_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273835.1"
                     /translation="MITIRNLSKTFSEQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQEFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            16323..16547
                     /locus_tag="VGY20_000972"
     CDS             16323..16547
                     /locus_tag="VGY20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ4273836.1"
                     /translation="MLVEIFSLYVKGLILSSLTMMLICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            16611..17528
                     /gene="trxB"
                     /locus_tag="VGY20_000973"
     CDS             16611..17528
                     /gene="trxB"
                     /locus_tag="VGY20_000973"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ4273837.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVSTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIKGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMIPVTGMVTDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            17697..19076
                     /locus_tag="VGY20_000974"
     CDS             17697..19076
                     /locus_tag="VGY20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ4273838.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNSLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            19246..20706
                     /locus_tag="VGY20_000975"
     CDS             19246..20706
                     /locus_tag="VGY20_000975"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ4273839.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAIIGGADATSNVRAGKRFGIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVSTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMALIDNIRKDTHGKSDLK"
     gene            20703..21527
                     /gene="nadE"
                     /locus_tag="VGY20_000976"
     CDS             20703..21527
                     /gene="nadE"
                     /locus_tag="VGY20_000976"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ4273840.1"
                     /translation="MTLQEEIIRQLGVKASIAPQEEIRKTVDFLKAYLRKHLFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIM
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
BASE COUNT         6607 a         3866 c         4589 g         6482 t
ORIGIN      
        1 gatgatttga aaaagcttat caaatagagc ttaacaaaat cgaaaatttt tggaggtaag
       61 aaatgaaatt tttcctagat acagctaatg tagccgctat taaagcgatt aatgaacttg
      121 gcgttgtaga tggtgtcacc accaatccaa ccattatctc acgtgaagga cgtgattttg
      181 aaacagttat caaagaaatc tgtgacatcg tcgatggccc aatcagtgct gaagtgacag
      241 ggttaacagc agatgctatg gtagaagaag ctagaagcat tgctaagtgg catgacaatg
      301 tcgtcgttaa aattccaatg accacagaag ggttaaaagc aacaaacatt ctttcaaaag
      361 aagggattaa aactaatgtg accttgattt tcacagtaag ccaaggttta atggcaatga
      421 aagcaggcgc aacttacatt agcccattta tcggtcgttt ggaagatatt ggcacagatg
      481 cttatcaatt aatcagcgac cttcgtgaaa tcattgactt gtacgatttc caagcagaaa
      541 tcatcgcagc aagtattcgt acaacagccc atgtggaagc tgttgctaaa ttaggcgcac
      601 acattgcgac cattccagat cccttgtttg ctaagatgac gcaacatccc ttaacgacaa
      661 atggtttgaa aacctttatg gaagattggg cttcctttaa aaaataaggc taaagagtct
      721 gtggccaacg tgtcaacagg ctttcttatt tgatagtcaa aaagaaagtg atatgagctt
      781 attccataac gtttttagtt atttttctag cagaaaagaa tttagaaact ttagaggatt
      841 ttctgaaaaa aacgtctatt tttgtgatac aatagacaat agaaaacgtt taattttagt
      901 tgtaataaac agagaggttt tacttatgac atttgatgca attgaccagt tggcagtaaa
      961 cactgtccgc accctatcaa tggatgccat tcaagcggca aattctggac acccaggact
     1021 tccaatgggg gcagcaccta tggcttatgt tctttggaat cacttcatga acatcaatcc
     1081 caaaacaagc cgtaattggt caaacagaga ccgttttatc ctatcagcag gtcatggaag
     1141 tgccatgctt tatagcttgt tgcatttagc aggttatgat ttatctgtag aagatttaaa
     1201 gaacttccgt caatggggtt ctaaaacacc aggtcaccca gaagtgaacc acacagacgg
     1261 tgtcgaagca accacaggac ctcttggtca agggattgca aatgccgttg gtatggccat
     1321 ggcagaagct cacctagcag ctaaatttaa caaaccaggc tttgacatcg ttgatcacta
     1381 cacatttgct ttgaatggtg acggtgacct tatggaaggg gtcagccaag aagcagcaag
     1441 tatggcagga catttaaaac ttgggaaatt ggtcttgcta tatgattcaa acgacatctc
     1501 tcttgatggt ccaacctcta tggctttcac agaagatgtg aaaggacgtt ttgaagctta
     1561 tggttggcaa catatccttg tgaaagacgg aaacgattta gaagaaattg cagctgctat
     1621 tgaagcagct aaagctgaaa ctgagaaacc aaccatcatc gaagttaaaa ccattattgg
     1681 ttttggtgcc gaaaaacaag gaacttcagc tgttcacggc gctcctcttg gagcagaagg
     1741 cattgctttt gccaaaaaag cttaccaatg gactcatcaa gattttgaag tgcctgctga
     1801 agtaacagag cgctttgctc aaggtcttca agcgcgtggt gaaaaagcag aacaagcctg
     1861 gaatgacttg tttgcagctt atgaagcaga atatccagaa ttagcagcag aataccaaaa
     1921 agcctttact aacgaagctg ctcaagtaga acttgaagcg cacgaacttg gtagttcaat
     1981 ggcgagtcgt gtatccagtc aacaagccat ccaacaaatt tcagaacaag tagcctcttt
     2041 ctggggtggg tcagcagacc tttcagcttc aaacaatact atggttaagg ctgaaacaga
     2101 tttccaacca ggtcactacg aaggccgtaa catctggttt ggcgttcgtg aatttgccat
     2161 ggctgcagcc atgaacggta ttgcccttca cggcgggaca cgcgtttacg gcggaacctt
     2221 ctttgtcttc tcaaactatc ttcttccagc tgttcgtatg gcagcgctcc aaaatttgcc
     2281 aacagtttat gtcatgacac atgattctat cgctgttggt gaagatgggc caacgcacga
     2341 accaattgaa caattggcaa gtgttcgctc aatgcctaac ttgaacgtta ttcgtccagc
     2401 agacggtaac gaaacaaata ctgcttggaa acgtgccatt gctgaaacag atcgcccaac
     2461 tatgcttgta ttgacgcgtc aaaaccttcc agtgcttgaa ggaacaaaag aattggcaga
     2521 agatggtctt aacaaaggag cttacatttt atcagaagcc aaaggcgacc ttgatggtat
     2581 catcattgca acaggttcag aagtgaaact ggcaatggat actcaagaag ctcttgaagc
     2641 agaaggcatt catgtgcgtg tggtatctat gccatcacaa aatatctttg acgaacagtc
     2701 agcagagtat aaagaaagca tcctaccagc agctgtgact aaacgtctcg ctatcgaagc
     2761 aggttcaagc tttggctggg caaaatatgt cggcctagca ggaaaaacat tgaccattga
     2821 cacttggggt gcttcagctc caggaaatcg tatcttcgaa gaatatggtt tcactgtggc
     2881 aaatgcaact gagttataca aatcactcta ggttacacat cccaaacaga gtaaatcatt
     2941 tgatttactc tgttttttta aaataaaata cccagttctt tcttttcctc aaactgccta
     3001 atacacttct tttgataaga agtcattgac aaaacaattc ataaaaatta taatttaatt
     3061 gttattgtat tagcgtaaaa gtacaaaaat aaaggagtat aaatggaaaa gaaacgacag
     3121 cgcttatacg atgtgattag acacgcttat gattatccag aaaatcgtga aaatgtggcc
     3181 ttgtcgcagc tacttttagc agcttctaat agattaatta aacacagtaa cccacttctt
     3241 gttgcctacc aactaaacca agatgtggat aattacttac ttgataatga cattttactt
     3301 ccaaaatcgc tttgccgttt taagcagtca ttggaaaagt atattttaga ggaagcctca
     3361 tcagtctagg gagaaaaaga gaagaaaagg aaaaaggata ccatgataga atttaaacac
     3421 gtttcaaagt tgtatggcga caaagaagct cttagcgatt taaacgtcac catcaatgac
     3481 ggtgaaattt ttggcctcat cggacataat ggtgcaggta aaaccacgac catcagcatt
     3541 ttgacttcta ttatcgaagc gagttatggc gaagtatttg tggatggtca acttctgaca
     3601 gaaaatagag aagctatcaa aaaacaaatt gcttatgtgc cagattctcc tgatattttc
     3661 ttgaatctaa ctccaaatga gtattggcag tttttagcta aaatttatgg ggtgtcagat
     3721 gaggaccgag aagaacggtt agcacagctg acaacacttt ttgaactaaa ggaagaagtt
     3781 aatcaaacca ttgatagttt ctcacatggg atgcgccaaa aagtgattgt gattggtgct
     3841 cttgtatcaa atcctaacat ctggattttg gatgaaccat taacaggttt ggatcctcag
     3901 gcttcttttg accttaagga aatgatgaaa gctcatgcag cttctggaca tacggtctta
     3961 ttttcgactc acgtgctatc agtcgctgag cagttatgtg atcgtatcgg gattctaaaa
     4021 aaagggaaat tgatttttgt tggaacgatt gatgagttaa aagaacacca tcctgacaaa
     4081 gatttggaga gcatctacct tgaactcgca gggcgtaagg cgcaagaaga ggggtgatgg
     4141 gatgaattgg tctactattt gggaacttat caagataaac attctttatt ccaatccaca
     4201 aagtttagct aacctcaaaa agcgtcagga aaaacacccg aaagaaaact tcaaagccta
     4261 taaaagcatg atgaggcaac aggccttaat gattgccatg tttttggtga tttacctttt
     4321 tatgtttatt ggtgttgatt tcagtcatta tccaggactt ttctcctttg acgttgctat
     4381 gttttttatc atgtcaacct tgacagcctt tagctctctt tacaccattt tttatgaaag
     4441 taatgaccta aaactttata ttcacttacc agtaacgtca gaagaactct atattgcaaa
     4501 aattgtctcg tcgctaggga tgggtgctgt ctttttgatg cctcttatct ctcttctctt
     4561 aattgcctac tggcaactat tgggaaatcc tttgtctatt ttagtagcta tcgtgctctt
     4621 tttagtgtta ctagtgagtt cgatggtatt agctatttat atcaatgctt gggtgggcaa
     4681 aatcattgta aggagtcgca agcggaaact catttcaact atcatgatgt ttgtctcaac
     4741 ttttggagct ttcgtcttaa tttttgcgat taatattagt aacaacaagc gtacgatgac
     4801 ggatggcgta tttactgatt acccaactat tccgtatttc aaaggattct atgatgttgt
     4861 tcaggcaccg ttttctactg tggccctcct taacttttgg ttcccattgt tacttatcct
     4921 agctatggta tatggtattg tcacaaaagt gatgccaact tattatcgtg aggtttttta
     4981 tattagtaac gagaacaaag tcaagcaaac taaaaaacca gttaatcgtc ctcatcagaa
     5041 tcaatcactg gcgcagttgt tgcgaaaaca tcacctatta acgttacaaa atgcaacttt
     5101 actgacacaa acctatctca tgcccctgat gtatgtgatg ctttttatcg gtccaagttt
     5161 gtcacgtggc acaggtttct ttaagcatat ttccccagat tactttgggg tggccttatt
     5221 atttggggtt agtttgggtg tcatgtgtgc aacaccaacc agctttattg gagtaggtat
     5281 ttcacttgaa aaggataact ttacctttat taaaagctta ccaataacgt taaaaaaatt
     5341 cttgctggat aaattttgct tgcttgtagg tctgcaactg attgtgccta tggtgattta
     5401 tcttgtgttt ggtctctttg tgttgcacct ccatcccctg ctaacgattg ctttttgtct
     5461 tggctacgcc ctatcactga ttgtgcaagg ggaattgatg tatcgtcgtg actaccggct
     5521 tctggatata aaatggcaag acatgacgca actctttacc agaggagatg ggcaatggtt
     5581 aacaatggga cttatctttg gtaatttaat agttgcaggt gtgctaggtt ttggagctgt
     5641 tatcattgcc aatattatcc aacaacctct gttgattagt attctattga gttgtctaat
     5701 actaatggtt ttaggtcttg cgcaattatg gattcaaaaa accttctgga aaagcttaga
     5761 gaggctttaa cgaagaggga tcattattcg gaaaaacagt cctctggggc tgttttttat
     5821 gctataattt gggtatgatg aaacgacaat ttgaagatgt aacacgtatc gtaattaaaa
     5881 ttggcactag ttcattagtg ctaccaacag gtaaaatcaa tttagaaaaa atcgaccaac
     5941 tggcttttgt gatttcaagt ttgatgaata agggcaagga agttatttta gtatcatcgg
     6001 gagcgatggg atttggacta gatattttga aaatggaaaa acgtccgacc aatcttgcta
     6061 aacaacaggc agtttccagt gttggtcagg tagccatgat gagtctttat tcacaaattt
     6121 ttgcacacta ccaaaccaac gtgtcacaaa ttttattgac gagagatgtg gtggttttcc
     6181 cagaaagtct agccaatgtt accaatgctt ttgaaagtct gattagtttg ggcattgtgc
     6241 cgattgttaa tgaaaacgat gcggttagtg tggatgaaat ggatcatgcg actaaatttg
     6301 gagataatga ccgtttatct gctgttgttg ctggtattac aaaagctgat ttattaatca
     6361 tgttgtctga tattgatggc ctttttgata aaaaccctac tatttatgag gatgcgcagc
     6421 tacgcagcca tgttgcagtc attacgcaag aaatcattgc gtcagcaggt ggtgcgggca
     6481 gtaaatttgg cacaggtggg atgcttagca aaattcagtc tgctcagatg gtatttgaaa
     6541 acaaaggaca gatggtcttg atgaatggag ctaatcccag agatatttta agagtgcttg
     6601 aagggcaacc actaggcacc tggttcaagc agatagaaga ggtaacacat gactgatatg
     6661 agaaggttag ggcaaagggc aaaacaagct agtcttttaa tagcaccttt gtcgacgcaa
     6721 attaaaaatc gctttttaag caccttagca aaggcattag ttgacgatac acagactctc
     6781 ttagccgcta accaaaaaga tttagcgaat gctaaagaac atggtatttc tgacatcatg
     6841 atggatcgct tgcgcttaac ttcagaacgg attaaagcga tagcacaggg agttcaacag
     6901 gttgctgact tagctgatcc aatcggacaa gtcattaagg gctatactaa cctcgatggc
     6961 cttaaaatcc tccaaaagag agttcctcta ggggtgattg cgatgatttt tgagagtcgc
     7021 ccgaatgttt cagtggatgc ttttagtctg gcgttcaaaa ccaataatgc catcatccta
     7081 aggggaggaa aagatgccct tcactcaaat aaagctttgg tgaagttgat ccgacaaagt
     7141 ctggaaaaat cagggatcac cccagatgct gtccagctag ttgaggatcc ctcacatgct
     7201 gttgctgaag aattgatgca ggcaacggat tatgtagatg ttttgattcc acgtggtggt
     7261 gctagattaa ttcagaccgt taaggaaaaa gcaaaagtgc ctgtcatcga aacaggtgtg
     7321 gggaatgtgc atatctatgt ggatgctcag gctgacttag atatggctac taagattgtt
     7381 atcaacgcta aaacaaggcg cccaagtgtt tgcaatgcag ctgagggatt ggtaattcac
     7441 gaagctgtag cagcaaggtt tattcctatg cttgaaaaag cgatcaatca ggttcagcca
     7501 gtagaatggc gggcagatga caaggctttg ccgttatttg agcaggcagt gcctgcaaaa
     7561 gcagaggatt ttgagaccga atttttagat tatatcatgt ctgttaaggt tgtttcctct
     7621 ttggaagaag ctatttcttg gataaatcag catactagtt accattccga agctatcatc
     7681 acaagggata ttaaggcagc agagaccttt caagatttag tggatgctgc tgctgtttat
     7741 gtcaatgctt caacacgatt taccgatggt tttgtttttg ggcttggtgc tgaaattggg
     7801 atttctactc aaaaaatgca cgcccgtggg ccaatgggct tagaagcctt gacaagcact
     7861 aaattttata ttaatgggga tggacatatc agagaataag ctactagaag gtcccttaat
     7921 gactaatgaa agagaaaaag aagaagcaaa ggttgcttta gaagaacttt tacatcagat
     7981 tcaggtgatt catttggaat tgaaaattgt ccgaaattgg ggctattttg tttttttgga
     8041 gacggtatgc ttgtcagctg gatcaaacgt cgtgagcaag gttaaacaat tattaataga
     8101 cttacaagtt actcttcaaa actgccgaag atagctggca gacgtcgata ggctattatt
     8161 agctgactta ccaaatatga gttgggataa aacctttggt atttggttag acaacctgct
     8221 gacagattgg tgtgtgatta aggaagctaa aaatctttta caagcagttg aaattctaga
     8281 ggcagcggct aagtcagtgc ttgctaagtt ggctgatagc gaacaaaaag aacaagatta
     8341 gagaagagga agattcccac gagaatgaaa tccttttctc ttactttttc atttctaaac
     8401 cttttgaagt atggtacaat taaagttatg acaaaagaat ttcatcacgt gaccgtactc
     8461 cttcacgaaa cagtggacat gcttgacata aagcctgatg ggatttatgt tgatgcgacg
     8521 ctaggtggct caggccactc agcttatttg ttgtccaaac ttggtgaaga agggcacctc
     8581 tattgttttg accaagacca aaaggctatt gacaatgcac aagttaccct caaatcttat
     8641 attgacaaag gacaggtaac ttttattaaa gataatttta gacacctcaa agcacgttta
     8701 acagcgcttg gagttgatga aattgatggt atcttatacg accttggtgt ttccagcccg
     8761 caattggatg aaagagaacg agggttttct tataaacaag atgctccatt ggatatgcgc
     8821 atggatcgtc agtcgctctt aacagcttac gaagtggtga atacctatcc attcaatgat
     8881 ttggttaaga tttttttcaa atatggtgaa gataaattct ccaagcagat cgctcgaaaa
     8941 attgaacaag caagagctat taagcctatt gagacaacaa cagagttggc agaattgatt
     9001 aaggcagcaa agccagctaa agagttgaag aaaaaaggcc atcctgctaa acagattttt
     9061 caagctattc gcattgaagt caatgatgaa ttgggagcgg ctgatgaatc tattcaggac
     9121 gctatggaat tattagccct tgatggtcgt atctcagtca ttaccttcca ttctctggaa
     9181 gatcgcctaa ccaagcagtt gtttaaagaa gctagtacgg tggatgtgcc aaaagggctt
     9241 cctctaattc ctgaagatat gaaacctaag tttgaacttg tttcacgtaa gccgatctta
     9301 cctagtcatt cagagttaac agctaataaa agggcacact cagccaagct acgtgttgcc
     9361 aaaaaaattc ggaaataagc ctatgacaaa tgaaaaaaga actcaggcag ttacaaatgc
     9421 cttgcaaaaa cgaataaaaa ctttctctag aattgagaag gctttttata ctgctatcat
     9481 tgtaacagcg attacgatgg cagtaagtat tatttacctg caaagtcgta agttgcagct
     9541 acaacaagaa atcactagtt taaatagtca tatttcggat caaaaactag agcttaataa
     9601 tgccaagcaa gaagtaaatg agttatctcg ccgtgatcgt attatagata ttgctggtaa
     9661 agcaggctta agcaaccgta ataataatat caagaaagtc gagtaatcta tgaaaaaatg
     9721 gcaaaaatat gttttagatt atgttgtgcg cgataggaga actccagtcg aaaatcgcgt
     9781 tcgagttgga caaaatatga tgctcttaac tatctttatt ttctttattt tcattattaa
     9841 tttcatgatt attattggaa cagatcaaaa gtttggagtt agtttgtcag aaggggcgaa
     9901 gaaggtttat caagaaaccg ttacgatcca agctaagcgt gggaccattt atgatcgaaa
     9961 tggtacagct attgcagtgg attctacgac ttatagcata tacgcaattt tggataaatc
    10021 atttgtctcg gcttcagatg aaaagttata tgtacaacct tctcagtatg aaacagtagc
    10081 tgatatttta aaaaagcatc tgggaatgaa aaaaacagat gtaattaaac agcttaagcg
    10141 taaaggactt ttccaagtct cgtttggacc gtcagggtct ggcatttcat atagtactat
    10201 gtctactatt caaaaggcta tggaagatgc caaaatcaag ggaattgctt tcacaactag
    10261 tcctggtcgt atgtatccaa atgggacatt tgcttcagaa tttataggcc tagcatctct
    10321 aacagaagat aaaaagacag gtgttaagag tttagttgga aaaacaggtc tagaagcttc
    10381 ttttgataaa attttatcag gtcaagatgg tgttattact tatcaaaaag atcgaaatgg
    10441 gaccacactc ttgggtacag gtaagactgt caagaaagct attgatggca aagatattta
    10501 cacaacgcta tctgagccta tccagacctt cttagaaacc cagatggatg tttttcaagc
    10561 caaatcaaat ggtcagttgg ccagtgcaac acttgttaat gctaaaactg gtgaaatttt
    10621 ggcaacaaca caacgcccca cttataatgc tgatactctg aaaggacttg aaaatacgaa
    10681 ctacaaatgg tacagtgcac ttcatcaagg aaattttgag ccaggctcaa ccatgaaagt
    10741 gatgactctg gcagcggcta ttgatgataa agttttcaac ccaaacgaaa cctttagcaa
    10801 tgctaatggt ttaacaattg cagatgctac tattcaagac tggtctatta acgaaggcat
    10861 ttccacagga cagtacatga attatgcaca agggtttgcc ttctcaagta acgttgggat
    10921 gactaaactt gaacaaaaaa tgggtaatgc aaaatggatg aattatttga cgaagttccg
    10981 ctttggtttt cctactcgtt ttggtttaaa agatgaagac gcaggtatat ttccttctga
    11041 taatatcgtg actcaagcta tgagcgcttt tggtcaaggg atttctgtaa cccagattca
    11101 aatgcttaga gcctttactg ctatttctaa taatggtgag atgttagagc cacaatttat
    11161 cagtcaaatt tatgatccta acacagcaag ttttagaacg gcaaataaag aaattgttgg
    11221 aaaacctgta tcaaaaaaag ccgctagtga aacaagacag tacatgattg gtgtaggaac
    11281 agaccctgag tttggaacac tctattcaaa aacatttgga ccaattatta aagtgggtga
    11341 tttacctgtt gctgttaaat caggaacagc acaaattggt tcagaagatg gaagtggtta
    11401 tcaagatggt ggattgacta actatgttta ttcggttgtg gcaatggtgc cagctgataa
    11461 accagacttt ttgatgtatg ttactatgac taaaccacaa cattttggtc cccttttttg
    11521 gcaagatgtg gttaacccag tattggaaga agcatactta atgcaagata cactaactaa
    11581 gccagtagta tcagatgcta atcgtcaaac aacttataaa ttaccaaact ttgtaggaaa
    11641 gaatcctggt gagacatcaa gcgagttgcg tcgaaatctt gtccagccag ttgtccttgg
    11701 tactggcagc aagatcaaaa aagtatcgca tcagcccggt caaacgttaa cagaaaacca
    11761 acaagttctc atattatcag accgttttgt ggaggtacca gacatgtatg gctggacaaa
    11821 atccaatgtt aaaacctttg ctaaatggac tggaatagac atcagcttta aaggaacaga
    11881 ttctggtcgt gttatgaaac aaagtgttga tgttggtaag tccttgaaaa aaataaaaaa
    11941 aatgaccatt actttaggag attaacatgt ttttaaccct tattgcagca atcatatcct
    12001 ttatggtatc tgctttcaca atgccttatt ttatcaaatt ctatcaattg aaaaaaattg
    12061 gtgggcaaca aatgcatgaa gatgtcaagc aacatttagc taaagctgga acaccgacta
    12121 tggggggaac tgtttttctt ttagtagcta cggcagtatc gttattggtt agtctatttt
    12181 cgatcaaaaa cactcaaagt ttagctttga tttcaggcat tttatcaatt gttgtcattt
    12241 acgggattat cggatttttg gatgattttt taaaaatttt taagcaaatc aatgaaggat
    12301 tgacagccaa acaaaagtta gccttgcagt tggttggagg attgatgttc tattttttac
    12361 atgttagtcc aagtggcatt tcctctatta atgtgtttgg ttatcagctg ccgctgggaa
    12421 ttttctatct attctttgtt ttgttttggg tggttggttt ttcaaatgcc gttaatttga
    12481 cagatggtat cgatggctta gcttctatct cagtggtgat tagtctagtg acttacggcg
    12541 ttattgccta cgttcagagt caatttgatg ttttgttact gattggagca atgattggag
    12601 ccttgcttgg cttcttctgc tttaatcaca aacctgctaa agtatttatg ggagatgtag
    12661 gtagtttagc ccttggagct atgttggctg ccatttctat tgcgcttcgt caagaatgga
    12721 ctctcctgat tattgggatc gtttatgttc ttgaaacaag ttctgtgatg ttgcaagtgt
    12781 cctatttcaa gtacaccaag aaaaaatatg gagaaggtcg tcgtattttt agaatgacgc
    12841 ctttccacca ccatttggaa ttgggaggct tgtctggtaa aggcaagaag tggtcagaat
    12901 ggcaggtaga tgcttttttg tggggagtag gaagtctagc tagtttacta gttttagcta
    12961 tcttatacgt gttctaaggg ggattatcaa gaatagtcac caatagcatt ttgattaaca
    13021 tcatcaataa gagagtggag atgacccaca aatgctgatt ttgtgagtgt tgtcccactc
    13081 cttttgcttg tctctaacaa taatgatata atatggaaga aaacgaggta aaacatgtca
    13141 tttaaagatt atcattttaa gcaatacgtt cagcaagcgc tggaagaaat tggctttgtc
    13201 aatccaacag aggtccagaa gcggctgatt cctattgtta actccggtcg tgacttagtt
    13261 ggggaatcaa aaacaggttc agggaaaacc cacacctttt tgttgcccat ttttgaaaaa
    13321 cttgatgaag ctaaagcaga agtgcaagtc gttattacag cacctagccg tgaattagca
    13381 actcaaattt ttgatgcttg taaacagatt gctaagcact cccaagaaga gattcgtttg
    13441 gccaactatg ttggaggcac agacaaactc cgtcagattg aaaaactaaa aggcggccag
    13501 ccgcatattg tcatcgggac cccaggtcgt atttatgact tggtcaagtc tggtgattta
    13561 gcgattcata aagcaacgac ctttgttgtg gatgaggccg atatgaccat ggacatgggc
    13621 tttttggata cggtagataa gattgcagcc tctcttccaa aatctgttca gattttggtg
    13681 ttctcagcaa ctattccaca gaaattacaa ccctttttga aaaaatactt gaccaatcct
    13741 gttattgaac aaatcaagac aaagaccgtt atcgcagata ccattgataa ctggcttgtg
    13801 tcgacaaaag ggcgtgataa gaatggtcaa ctgttagaga ttctcaaaac catgcagcct
    13861 tatatggcta tgctttttgt caataccaag gaacgcgcag atgatttgca tgccttttta
    13921 acagctaatg gcttaaaagt ggctaagatt catgggggca ttcctccaag agaacgtaag
    13981 cgcatcatga accaagtcaa aaaactggac tttgaataca ttgttgccac agacttagca
    14041 gcgcgtggga ttgacattga aggggtcagc catgtgatca atgacgccat cccacaagac
    14101 ttgtctttct ttgtccatcg tgtaggccga acaggtcgaa atggtatggc aggaactgcc
    14161 attacacttt accagccaag tgacgactca gatatcaaag agctggaaaa aatggggatt
    14221 gcctttacac cgaaggtgct caaaaatgga gaatttcaag atacctatga ccgtgatcgt
    14281 cgtcaaaacc gtgaaaaagc ctatcaaaaa ttagacacag aaatgattgg tttggtcaaa
    14341 aagaaaaaga aaaaagtcaa accaggttac aagaaaaaaa ttcagtgggc ggttgatgaa
    14401 aaacgtcgca aagaacgtcg tgctgaaaat cgtgctaagg gccgcgctga gcgtaaggcc
    14461 aaaaaacaac atttttaaga acaaagagca agcaagtgat caattggcat ctaatcttgc
    14521 tttttttaca cctgctactt gtattgttta ccatttgtta acacaaaaag tgatcatagc
    14581 ttttgcttat tgctataata cggatttact attttacatg tggatctaag agtgttagaa
    14641 tattaagaca aacaaataca aaggggaaat acatgacatc agtgttttta accagtggtt
    14701 gggcttttta tgatcacctc atctcaccga ttccacatgg gaaacttttt agttggcatg
    14761 ctgtttttga tgccattcca aatattatcc aacggcttcc aattacgctt ggtttgacac
    14821 tatcaggagc aacctttggc ttggttttgg ctttgatttt tgcccttgtc aaaattaata
    14881 aagtgaagct actataccct attcaagcta tttttgtgag tttcttgcga ggaactccta
    14941 ttttggtaca gttgatgttg acctactacg gtattcccct ctttctaaaa tgtctcaatc
    15001 agaagtatgg ctttgattgg aatgttaatg caattccggc ttctattttt gccattacag
    15061 cctttgcttt taatgaagca gcctacgcta gtgaaactat tcgggcagcc attttatcag
    15121 tagatacagg tgaaattgaa gcagcaaaaa gtctaggcat gacttctgtg caggtttacc
    15181 gtcgtgtcat tattcctaat gcaactgttg tggctattcc aaccttgatt aatgggttaa
    15241 ttggcttaac taaaggaaca tcgcttgcct ttaatgcagg gattgtcgaa atgtttgccc
    15301 aggctcaaat tctaggcgga tcagattacc gttattttga acgttatatc tctgttgctc
    15361 ttgtctattg gtctatcagt attttgatgg agcaagtagg tcgcttgatt gaaaacaaga
    15421 tggccatcaa agcaccagaa caagctagaa atgaaaagtt aggagaattg cgttgatgat
    15481 tacaattaga aatttgagca agacattctc agagcaaaaa gttttagata gtttggctct
    15541 tgatattgaa aaaggtcaag tgattgcttt agttggtgct tcaggagcag ggaaatcgac
    15601 tttccttcgt agcctaaact atttggaaaa accagattca ggctccatta gcattggtga
    15661 ctttacggtt gattttgaga ccattaccac agagcaagtc cttattttac gtcggaaact
    15721 tgccatggtt tttcaagaat ttaatctttt tgagcgtcgt acagcccttg agaatgtcaa
    15781 agaaggtctt aaggtggtca aaaaactgtc tgaccaagaa gcgactaaac tggcgcaagc
    15841 agaattggct aaggttggtt tagcagatcg aaaacatcat tacccacgtc atttatcagg
    15901 gggacaaaaa caacgggtag ctttagcaag agctctggct atgaaaccag atgttttatt
    15961 actagatgag ccgacctcgg ctcttgaccc ggaattagtt ggcgaagtgg aaaagtctat
    16021 tactgatgct gctaaatccg gacagaccat ggttttagtg agtcacgaca tgaattttgt
    16081 ttaccaagtg gcagatagag tactcttttt agatcaaggg aaaatcttgg aacaaggaac
    16141 acctgaagaa gtctttagac atcctcaaaa agaacgaacc aaagaatttt ttgcaagcta
    16201 ttcaaaaaca tacatttaat gttattcttt tgttataata aaaaggtcta ggtattcctg
    16261 ggcttttctt gttatgtttt ttagaaaaac ttatgggaaa gaaataatga ctaggtggaa
    16321 atatgttagt agaaatcttt agcctttatg taaaaggtct cattctctca agccttacta
    16381 tgatgttaat ttgtttaata tggactttat ggcgagcaaa aaccaaaaga gataaaacat
    16441 tgattgaaag acaagctttt ctttatgaga tgctaatggt tgctatttta accatcccaa
    16501 ttttatcgtt tgcttttatg agtattttgg ttgtcataaa atcctaatca ttgcaagaag
    16561 ggattatcat tgctaaaata ttggcaagag tagattaaag gagaaaaatc atgtacgata
    16621 cattaattat tggttcagga ccagcaggaa tgactgccgc tttatacgct gctagaagta
    16681 atttatctgt tgccattatt gaacaagggg cacctggtgg tcagatgaac aacacttttg
    16741 atatagagaa ttacccggga tatgatcata tatctggacc agaattagct atgaaaatgt
    16801 acgaaccttt agagaagttt aacgttgaaa acatttatgg tattgttcaa aaaatagaaa
    16861 attttggtga ctataagtgt gtgtcgactg aagatgctag ttatgaagct aaaacggtta
    16921 ttattgcaac gggagctaaa taccgtgtac taggtgttcc aggtgaagaa tactatacta
    16981 gtcgaggagt ttcttattgt gcagtatgtg atggtgcgtt tttccgtgac caggatcttt
    17041 tggtcgttgg tggtggtgac tcagcagttg aagaagctat ttatcttact cagtttgcta
    17101 aaaaggtaac cgttgttcat cgccgtgatc aactaagagc tcagaaaatc ttgcaggacc
    17161 gtgccttcgc taatgacaaa gttgacttta tctgggattc agttgtcaaa gaaatcaaag
    17221 gtaacgacat taaggtctcc aatgtcctaa tcgaaaatgt gaaaacaggc caagtcactg
    17281 accacgcttt tggaggtgtg tttatctatg ttggtatgat tcctgtgaca ggtatggtaa
    17341 cggatctaga aattaccgat caagaaggtt ggattatcac agatgatcat atgaggacaa
    17401 gtattcctgg tatttttgcg attggagatg ttcgtcaaaa agaccttcgc caaatcacga
    17461 cagctgttgg agatggtgca attgcagggc aaggtgttta tcactacctt gaaagtttcc
    17521 cttcataagg ttaaaactcc ttcatttttc cttgtctttc ccttaaaaaa gctaaattgc
    17581 ataagacctt taagaaatat aaagaacagt tttccttaat atctttccta gataaatgtt
    17641 gaaaattttc tgaaaaaagg atataataaa tagtatttta gtgaatggag tttagaatgt
    17701 ctataaaaga acaaacagac aataacgaac tagaaaatgg aatggtaagg ggcttagaaa
    17761 atcgtcatgt gcagttaatt gcgattgcag gaacgattgg aacaggtctc tttttagggg
    17821 caggacgctc tattgcccta acaggaccat ctattatttt tgtgtatatg attacaggtg
    17881 ccttcatgtt tatgatgatg cgtgccatcg gtgaaatgct ctattatgat ccagatcaac
    17941 acacctttat caattttatt tccaaataca ttggtccagg ctggggttat ttttcgggtc
    18001 tatcctattg gatttccctt atttttattg gaatggcaga aatcacagca gtaggtgctt
    18061 atgtgcaatt ttggttccca agctggccag cctggttgat tcagttggtt ttcttagtct
    18121 tacttagttc gatcaactta attgctgtgc gcgtttttgg ggaaacagag ttttggtttg
    18181 ccatgattaa gattttagct attttagctt tgattgcaac agctattttc atggtattga
    18241 caggttttga aactcacaca ggccatgcta gcctttccaa tatttttgac catttttcca
    18301 tgttcccaaa tgggaaacta aagttcttta tggccttcca aatggttttc tttgcttatc
    18361 aagctatcga atttgtgggg attaccactt ctgagacggc taacccaaga aaagttctac
    18421 caaaggctat tcaagaaatt ccaacccgta ttgtgatctt ttatgtggga gccttggtct
    18481 ctattatggc aattgtgcca tggcatcagt tgccagttga tgaatctcct tttgtgatgg
    18541 tgttcaaatt gattggtatt aaatgggcag cagccttgat taactttgtg gtcttgacat
    18601 cagcagcgtc agcgcttaac tcaacccttt attcaaccgg tcgtcatctc tatcagattg
    18661 ctaatgagac tccaaacgct ttgaccaata gcttaaagat taatacttta tctcgacaag
    18721 gggtgccaag tcgtgccatt attgcttcag cggttgtggt tggtatctca gctttgatta
    18781 atattttgcc aggagttgca gatgcgttct cactcattac ggcctcctca tcaggtgtat
    18841 acattgctat ctacgcctta accatgattg ctcactggaa gtaccgtcaa tctaaggact
    18901 ttatggcaga tggttatttg atgccaaaat ataaagtgac aacgcctttg acccttgctt
    18961 tctttgcttt tgtctttatc tctcttttct tacaagaatc cacctatatc ggcgcaattg
    19021 gagcaacgat ttggattatt gtttttggga tttatagcaa tgtcaaattt aaataataat
    19081 tagcgatata gaactgaatc cattaacttt ttcttatttg aggatgttat gatggatcgg
    19141 ttctttttta ctttagcgat aactcaagtt atttctaaat cctagccaaa gagtcctttt
    19201 taaaatatgt tataatagta acaataattc aaaggagtcc cctttatgta taaagatgat
    19261 agtttaactc tccatactga tttgtatcag attaatatga tgcaagttta ttttgagcag
    19321 ggcattcata atcgtcatgc ggtgtttgaa gtctatttcc gcaaggaacc ttttaacaat
    19381 ggttatgctg tttttgccgg tttacagcgc atggtggagt acctagagca atttcaattt
    19441 tcagaaacag atttggctta ccttgaagaa ctgggttatc cagaaaactt tttgacctac
    19501 cttaaagagt tacgattaga attgaccatc cgttcagcta aagaaggaga tttggttttt
    19561 gctaatgagc cgattgtgca agtagaaggc cctctaggtc agtgtcagtt ggtcgaaaca
    19621 gctcttttaa acattgttaa cttccagacc ttgattgcta caaaggctgc tcgtattcgt
    19681 tctgtgattg aggatgaacc ccttttggaa tttggcaccc gtcgtgcaca ggaactagat
    19741 gctgcgattt ggggaacccg cgcagcgatt attggtggag cagatgcaac aagtaatgtg
    19801 agagcaggta aacgctttgg tatccctgtt tcaggtacgc atgctcatgc tttagtgcaa
    19861 gcctatggta atgattatga tgctttcatg gcttatgcta agacccataa agattgtgtg
    19921 tttcttgttg atacctatga cacacttaaa gtaggtgtgt caacagctat ccgagtagcc
    19981 aaagaaatgg gtgacaagat taattttcta ggtgttcgat tggactctgg tgacttggct
    20041 tacctctcaa aaacagtccg tcagcagtta gacgacgctg gttttaccga ggctaagatc
    20101 tatgcttcta atgacctcga tgaaaatacc atcctcaact taaaaatgca aaaggccaaa
    20161 attgatgttt ggggtgttgg gaccaaactg atcacagcct atgatcagcc tgccttaggt
    20221 gctgtttata agattgtctc tattgagcaa gaagatggta gcatgcgtga tacgatcaaa
    20281 ttatcaaata atgcagaaaa agtatccaca ccaggtaaaa aacaagtttg gcgcatcacc
    20341 agtcgtgaaa aaggcaaatc agaaggcgac tatatcacgt ttactgatat taatgttaat
    20401 gaattaactg agattgagat gttccatccg acctatacct atattaagaa aaccgtcaga
    20461 aattttgatg caattcctct cttagttgat atttttgtca agggagaatt agtttaccag
    20521 ctaccgacct tatctgaaat caagacttat gctaaaaaag aatttgacaa gctttgggat
    20581 gaatacaagc gtgtcttaaa tcctcaagac tacccagtag acttggcacg agatgtttgg
    20641 caaaataaga tggccttaat tgataacatt cgtaaagata ctcatggaaa gagtgattta
    20701 aaatgacctt acaagaagag attatccgac aattaggcgt caaggctagc attgccccac
    20761 aagaagaaat tcggaaaaca gttgattttt taaaagctta tctgcgtaag cacttatttt
    20821 tgaaaactta tgtcttaggc atttctggag gtcaggattc taccctcgca ggaaagctag
    20881 cccagatggc cattgcagag ttacgggagg aaacaagtga tcaagcttac caatttatag
    20941 ctgttcgctt gccttacggg gttcaggctg atgaagcaga tgcgcaaaag gccttggctt
    21001 ttatcatgcc agaccaaaca ctgactatta atattaaagc agctgttgat ggccaagtag
    21061 aggctctaca agcagcaggc gttgagattt ctgattttaa caaaggtaat atcaaagcac
    21121 gtcagcggat gatcagtcaa tatgccattg ctggccaaat ggctggtgct gttattggaa
    21181 cggatcatgc ggcagaaaac atcactggtt tctttactaa atttggtgat ggtggagcag
    21241 atattcttcc cctctttagg cttaacaaac gtcaaggaaa ggctcttttg aaagttttag
    21301 gagcagatgc tgctttgtat gaaaaagttc caactgctga tttagaagat caaaaaccag
    21361 gcttggctga tgaggttgct ctaggggtga cctatcaaga catcgacgac tatttggaag
    21421 ggaagctgat ttccaaagta gctcaagcaa ctattgaaaa atggtggcat aaaggacaac
    21481 acaagcgtca cctgccaatc accatttttg atgatttttg gaaataagag atgatgttca
    21541 aaga
//
LOCUS       DAUCWO010000028        20644 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUCWO010000028 DAUCWO010000000
VERSION     DAUCWO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.028"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            125..1108
                     /gene="guaC"
                     /locus_tag="VGY20_000977"
     CDS             125..1108
                     /gene="guaC"
                     /locus_tag="VGY20_000977"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ4273841.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDTPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLSALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1231..1326
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1413..1994
                     /locus_tag="VGY20_000978"
     CDS             1413..1994
                     /locus_tag="VGY20_000978"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ4273842.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            1994..3277
                     /locus_tag="VGY20_000979"
     CDS             1994..3277
                     /locus_tag="VGY20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ4273843.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSSAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFTS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(3341..4279)
                     /locus_tag="VGY20_000980"
     CDS             complement(3341..4279)
                     /locus_tag="VGY20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ4273844.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAYSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVLVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTVMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(4332..4517)
                     /locus_tag="VGY20_000981"
     CDS             complement(4332..4517)
                     /locus_tag="VGY20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ4273845.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            4655..5224
                     /locus_tag="VGY20_000982"
     CDS             4655..5224
                     /locus_tag="VGY20_000982"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ4273846.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            5259..6338
                     /gene="prfA"
                     /locus_tag="VGY20_000983"
     CDS             5259..6338
                     /gene="prfA"
                     /locus_tag="VGY20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ4273847.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRIDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            6338..7177
                     /gene="prmC"
                     /locus_tag="VGY20_000984"
     CDS             6338..7177
                     /gene="prmC"
                     /locus_tag="VGY20_000984"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ4273848.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLAKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            7161..7751
                     /locus_tag="VGY20_000985"
     CDS             7161..7751
                     /locus_tag="VGY20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ4273849.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPNHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            7769..8221
                     /locus_tag="VGY20_000986"
     CDS             7769..8221
                     /locus_tag="VGY20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4273850.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            8211..9467
                     /locus_tag="VGY20_000987"
     CDS             8211..9467
                     /locus_tag="VGY20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ4273851.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIARLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            9474..10451
                     /locus_tag="VGY20_000988"
     CDS             9474..10451
                     /locus_tag="VGY20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ4273852.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            10452..11051
                     /locus_tag="VGY20_000989"
     CDS             10452..11051
                     /locus_tag="VGY20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ4273853.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            11061..12785
                     /locus_tag="VGY20_000990"
     CDS             11061..12785
                     /locus_tag="VGY20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273854.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGATVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPSLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSPYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPISDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            12782..14497
                     /locus_tag="VGY20_000991"
     CDS             12782..14497
                     /locus_tag="VGY20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273855.1"
                     /translation="MIKTDHHLLKRMLHDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WFMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKVIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEEEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHVHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKERTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNGCYAR
                     LQKIE"
     gene            14738..16108
                     /locus_tag="VGY20_000992"
     CDS             14738..16108
                     /locus_tag="VGY20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ4273856.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(16267..17250)
                     /locus_tag="VGY20_000993"
     CDS             complement(16267..17250)
                     /locus_tag="VGY20_000993"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ4273857.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            17441..19927
                     /gene="gyrA"
                     /locus_tag="VGY20_000994"
     CDS             17441..19927
                     /gene="gyrA"
                     /locus_tag="VGY20_000994"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ4273858.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            19947..>20644
                     /locus_tag="VGY20_000995"
     CDS             19947..>20644
                     /locus_tag="VGY20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ4273859.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAP"
BASE COUNT         6375 a         3643 c         4287 g         6339 t
ORIGIN      
        1 gtttttacct aaagatacct ctaatcatca gaggtcatag gaagattatt ttgtatagag
       61 gacttagaga agaaggtgag aactgactta ttttctttca tctgtgctaa aagaggagaa
      121 acttatgttt aatgacatcc ctgtatttga ttatgaagat attcaactga ttcctaacaa
      181 gtgcattatt actagtcgtt cacaagcaga tacaagtgtg acactcggaa aataccagtt
      241 caagctacca gttatcccag caaatatgca aaccattatt gatgaaacaa ttgctgagca
      301 gttggcaaaa gagggttatt tttatattat gcatcgtttt gatgaagata gccgcaaacc
      361 ttttatcaaa cgcatgcatg aacaaggctt gattgcatcc atctctgttg gggttaaagc
      421 ttacgaatat gaatttgtga cttctttgaa agaggatact cctgagttta tcaccattga
      481 tattgctcat ggtcatgcta acagtgttat tgatatgatt aagcatatca aaactgaatt
      541 accagaaaca tttgtcattg ctggaaatgt tggtacgcca gaagctgtac gagaactcga
      601 aaatgcaggt gctgatgcta ctaaagtagg catcggtcca ggcaaagtat gtatcactaa
      661 agttaaaaca ggtttcggta caggtggttg gcagttatca gcactgcgct ggtgcgcaaa
      721 ggcagctcgt aagcctatca ttgctgatgg tgggattcgc acacatggtg atatcgctaa
      781 atccatccgc tttggagcta gtatggtcat gattggttcg ctttttgcag ggcatattga
      841 aagtcctgga aaaacagtag aagtgaatgg tgaaacgttt aaagaatatt atggctcggc
      901 gtcagcatat caaaaaggcg agcataaaaa tgtagaaggc aaaaaaatct tattgcctac
      961 aaaaggtcac ttatcagata ccctgaccga aatgcaacag gatttgcaat cgtccatttc
     1021 ttacgcgggt ggtaaagatt tagacagttt gcgtcatgtt gattatgtta tcgtcaaaaa
     1081 ttctatttgg aatggtgatt ccatttaaat gatatggagt tagctaggac cagtaaagaa
     1141 ttttttagct taatgctgga gatgctttta tcatatctct gtaggtattg aaaaaaataa
     1201 gaaacatggt aaaatagtaa attatcataa tgaattcaat aatgacatac ttatttatgc
     1261 tgtgaattgg cgcagcgtct ctacaagaca ccttaatgtc taacaataag taagctttta
     1321 ggcttgcttg gtttttaacc ttgcaaagtg agaaactctt atagtttctg gctttgcggc
     1381 aggctttttc tatttgaatc aaaaggagat ttatgcagtt acttgaagaa cgcatcttaa
     1441 cagatggcaa tattctagga gagaatattc taaaggtaga taatttttta actcatcaag
     1501 ttgattaccg gttgatgaaa gcaattggta aagtgtttgc tcaaaaatat gctgaggctg
     1561 gcattacaaa agtggttaca atcgaagctt caggtattgc accagccgta tacgctgcag
     1621 aagcaatgga tgttcctatg atttttgcga aaaaacataa aaacattacc atgacagaag
     1681 gcattttgac agcagaagtt tattctttca ctaaacaagt gacgagcacg gtgtctatcg
     1741 ctggtaaatt cctatctaaa gaagacaagg ttttgattat tgatgacttt ttagctaatg
     1801 gtcaggcagc caaaggcttg atcgagatta ttggtcaagc aggggcacaa gtcgtcggcg
     1861 ttggtattgt gattgagaaa tctttccaag atggtcgtcg attgattgaa gatatgggca
     1921 ttgaagtgac ctcattagca cgtattaaaa actttgaaaa tggtaatcta aacttcttgg
     1981 aggctgacgc ataatgacac attcaacaaa gcaggaacat tctcactcgc aatcagctgt
     2041 ccttggctta cagcatgtgc tttcgatgta tgccggatct attttagtac caattatgat
     2101 cgcaggtgct ttaggttatt ccgctagaga gttgacctac ctgatttcga cagatatttt
     2161 catgtgtggt gtggctactt ttttacagtt aaagttaacc aagcatacag gagtagggtt
     2221 accagttgtt ttaggctgtg cttttcaatc agtggcacct ctatccatta ttggagcgca
     2281 acaaggttca agtgccatgt ttggagccct cattgcgtca ggaatttatg tcattttagt
     2341 ggcaggtatt ttttctaaga ttgctcgttt cttcccacca attgttacag gatcggtcat
     2401 taccgttatt ggcttaagtc tcgtaggtgt tgctatgggg aatatgggag ataacgtcaa
     2461 agagcctacg gcacaaagca tgatgttatc attgttgacg attgtcatta ttcttttagt
     2521 ccaaaaattt accaaaggtt ttgtgaaatc catttccatt ttgattgggc ttgtagcggg
     2581 aactttggtt tcagctatga tgggattagt agatacaact cctgttgtag aggcttcttg
     2641 gattcatgtg ccaaccccat tttattttgg catgccaact tttgaaatca cttctattgt
     2701 gatgatgtgt atcatcgcaa cggtttctat ggttgaatca acaggtgtct acttagcgct
     2761 ttcagatttg actaatgatc aattagatga aaaacgtttg cgcaatggtt atcgctcaga
     2821 agggatcgca gtctttctcg gtgggttatt taataccttc ccttacactg gtttttctca
     2881 aaacgttgga cttgtccaaa tctcaggtat taaaacccgt cgtccgattt actacgcagc
     2941 aggtatcctt gtggtcattg gtctactccc taaatttgga gcaatggcac aaatgattcc
     3001 aagcccagtt cttggtggag ccatgcttgt tctctttggt atggttgctc ttcaaggaat
     3061 gcaaatgctt aatcgtgttg atttccaaaa gaacgagtat aattttatta ttgctgctgt
     3121 ttccatttca gcaggcttag ggtttaatgg caccaatctt tttactagct taccagagac
     3181 agctcaaatg ttcttaacga atggtattgt gattgccact ttgacatctg ttgtcttaaa
     3241 tttagtttta aatggtaaag acaaacaaga tgaataatta aaaaacggct aattgagccg
     3301 ttttttctgt gatcagtttt gagcaaaccg ttaacccctt ttaatgatag tatggttgaa
     3361 aatagtgtct caaaccattt gacataagga catggtgctt tcttgttact agaagacctt
     3421 caatattatc aaaggtgttt aaaagtgtga taatcattga gctatcaaga ccaaataaac
     3481 gggtagccca tatgtcacaa taaaaagaag aaggagccat gaccgttaag ctaagcatat
     3541 ctgtttctat agggtagcct gtctgacgat ctaagatatg atgatactgt ttccctttgg
     3601 ctgtaaactg cctttcataa attccagaag tcacaacaga gtgattgtta accttgatga
     3661 caccaagatg ttgcccacgt ttagcatctg gtttttgaat gccaattcga aaagtcttgt
     3721 cagggctttt cggattaggt ccatgaacta agacgtttcc acctaaatta ataagggcag
     3781 agtcaatacc atcttcaatc aggtaggcca taatcttatc agtaatataa ccttttgcta
     3841 aggctcctaa gtctaaagcc attcctactt gttgtaaaaa gacagtctgt tttttgtcat
     3901 ctataagaac ctgtctagga tctgttagag ctaactgttg tgagataagg ttatgactag
     3961 gcactctagc atcttcaaat ccaattcgcc aggcttgaac aagtggccca atggcgatat
     4021 taagattact tggtgttgac aagctataag ctttaccaat ttggattagg ttaaataaat
     4081 cagagtgaac gctgacgggt ttaacaccag ccgcctgatt aatagccatc aattcagagt
     4141 ttgaatcatt agctgaaaaa cgatttttgt aagtatataa taagtcaatg actctactta
     4201 gttgttggca agctttgtca gattcgatct gaatatcaat aactgttccc attagcttta
     4261 attgttgcgt aacaaacata attagtcctc tttttagcag aataccccca ctaaggagtg
     4321 ggggaaggtc attatgattt ttgtttcatt tcaccttgag gatagtaagt tccttcaggc
     4381 atatcgttga taaaaacatg gatattttct ttaggtgctt ttgcaatgcg tgagactact
     4441 tcggtaacct cgcgagctaa ttgatttttt tgttcttgtg agcgtccctc aaacaagtca
     4501 attgttacaa atggcataat gttactcctt tatgttattg ggcatcattg taacataatg
     4561 ctgtgaaaaa gtatacactg ttttcaatgg ctgggaaaac ttcagccttt gtggtaaaat
     4621 aggtagaata ctatagaaag taggattttc cgctttggct caactctatt ataaatatgg
     4681 gacaatgaat tctggtaaaa ccattgaaat tttaaaagtt gctcataact atgaagaaca
     4741 aggcaaacct gttgtcatta tgacaagtgc tcttgataca cgtgatggtt ttggcatcgt
     4801 ctcaagccgt attggtatgc gacgagaagc tattcccatt tcaaatgaca tggatatttt
     4861 cacatttatt gcccaattgg aggaaaaacc ttattgtgtc ctgattgacg aaagccaatt
     4921 tttaagcaag caaaatgttt atgatttggc gcgtgtggtg gatgagctaa atgttcctgt
     4981 catggctttt ggtttgaaaa atgatttcca aaacaatctt tttgaaggtt caaaacattt
     5041 gctattattg gccgataaga ttgatgaaat caaaacaatt tgccaatact gtagtaagaa
     5101 agcaaccatg gttttaagga cagaaaacgg aaaacctgtc tatgaaggcg atcagataca
     5161 aattggtggt aatgaaacct atatcccagt ttgtcgaaaa cattatttta atccagagat
     5221 ttaaaaggta atcagtttta aaggagatac aaagaaaaat gaatatttat gatcaattgc
     5281 aggcagttga agatcgttat gaagagttag gtgagttact tagtgatcca gatgttgttt
     5341 cagatacaaa gcgatttatg gaattatcac gtgaagaagc taatactcga gaaactgtca
     5401 cagcttatag agaatataag caagtcattc aaaccatttc agatgctgaa gaaatgatca
     5461 aagatgccag tggtgatcct gaattagaag aaatggctaa agaagaactt aaagaatcaa
     5521 aagcagctaa agaagagtat gaagaaaaat taaaaattct tctattacct aaagatccta
     5581 acgatgataa aaatattatt ttagaaattc gtggtgctgc tggtggcgat gaggcagcgc
     5641 tttttgctgg tgatctttta acaatgtatc aaaaatatgc tgaaactcaa ggatggcgtt
     5701 ttgaagttat ggaatcatcc gttaatggtg ttggtggtat taaagaagtg gttgccatgg
     5761 tttctggtca gtcagtttat tctaagctta aatatgaatc aggagctcat cgtgttcaac
     5821 gtgtcccagt gacagagagt cagggtcgtg ttcatacttc aactgcaact gttttggtaa
     5881 tgccagaagt tgaagaagta gaatacgaca ttgatcctaa agacttacgt attgatattt
     5941 accacgcgtc tggtgctggt ggtcagaacg tcaataaagt tgctactgca gttcgtatgg
     6001 tacacattcc tacaggaatc aaagttgaaa tgcaagaaga gcgtactcag caaaaaaacc
     6061 gtgataaagc gatgaaaatc attcgtgctc gtgttgctga ccattttgcg caaattgctc
     6121 aagatgagca agatgctgaa cgtaaatcaa cagttggtac tggtgatcgt tcagaacgta
     6181 ttcgtactta taatttccca caaaatcgtg taacagatca tcgtattggt ttgacacttc
     6241 aaaaattaga tactatttta tctggaaaaa tggatgaagt tattgatgca ctcgttatgt
     6301 atgatcagac aaagaaatta gaatctctca ataactaatg aactatgcta cattaatcag
     6361 aacttatgaa gataaactag aacaaataga tgaggatcgc gaaaatctcg cctatgtttt
     6421 tagagaaatc aaagaatggt caagtctaga tatgttgatt caccaaaatc aagctgtgac
     6481 tcctgaagat gctgttcttt tggaacatat tttctgttct ctaagtcagc atttatctcc
     6541 tcaatatatt acaggtaatg cttattttcg tgatttgaag cttgcagtcg acaagcgtgt
     6601 attaattcca aggccagaaa cagaagaatt agttgacatg attttagctg aaaatcttga
     6661 tgcaccgttg aatgttttag atattggtac aggtagtgga gcgattgcta tttctctcaa
     6721 aaaagaaagg tctaactggc aagtaacggc atctgatatt tctagagctg ctcttgactt
     6781 ggcaaaagcc aatgctgatg cttaccagct tgatataacg tttattgaat cagatgtttt
     6841 tagtctcatt tcaggaacat ttgatattat tgtgtcaaat cctccctaca tatcctacga
     6901 ggataaggaa gaagttagtc taaatgtttt gcaatcagag cctcatttgg cactttttgc
     6961 taaagaaaat ggctatgcta tttaccgtaa gattattgaa caagctgaca attacttagc
     7021 taaggagggg aagctttact ttgaaattgg ttataagcaa gctgaaggta taaaagatat
     7081 gttacaagcc tattttcctc aaagacatat acgagtcgta acggatatat ttggtaaaga
     7141 aaggatggtg gttgttgatt atggagtatc tagctagtat cattgaatca ggtgatgccc
     7201 ttgttttgcc aacagaaacg gtttatggtc ttttcgccaa ggctttagat gaaaaagcag
     7261 taaatgctgt ttatgactta aaacaaagac cccgagataa agcaatgaac cttaatgtgg
     7321 ctgattttaa tagtatctta gccttttcaa aagaacagcc gagatacctc aagaaattat
     7381 accaagcctt tttaccaggt ccgttaacga ttattttaaa agctaatgac caggttcctt
     7441 attggattaa ttcgggtctt tcaacggttg gttttcgctt accaaatcat ccaattaccg
     7501 cagctttaat tcaaaaaaca ggccctttaa ttggtccttc tgcaaattta tctggcaaag
     7561 ctagtggtcg agtttttgat catattatgc aagattttga ctttcaagtg tttggttatg
     7621 cagatgatcc ttttctaaca ggtaaggatt caaccatctt agacttatca ggtgagcgtg
     7681 ctgtgatttt acgtcaaggt gctatcacca aagaagaatt attagccaac gtgcctgaac
     7741 ttcgctttta agagaggagc cgcttggaat gttgagacca ttaagtaaaa cggactgtcc
     7801 tatgctacaa gaaattaacg ccaaagccct tggctattta gtttcgcttg acttgttaga
     7861 aaggcaatat gaacgattga ttgaggattg ccatcactat tttttagctt atgccgacaa
     7921 ggataccaat caattattag gctatgttca cgctgaacgt tatgagaccc tttatgcttc
     7981 tgatggtctt aatctcttgg gcttggctgt tttaccagcc tatcagagaa ggggaattgg
     8041 gagtgcctta ctaagggccc tggaatccca agctaggcag gaaggcattg cctttattcg
     8101 cctcaattct gccagtcatc gtaaagaagc gcatgctttt taccgaaacc tcgactatgc
     8161 agatgataaa acacaattac gattcatcaa aaacttacaa ggagatgaca atgatttttg
     8221 ataaaggtaa tgttgaagac tttgataaag agctttggga tgccattcat gctgaagaag
     8281 aaagacaaga acatcatatc gaattgattg catctgaaaa catggtttca aaagctgtca
     8341 tggcagctca aggttctgtt ttaaccaata agtatgcaga aggctatcca ggtaatcgct
     8401 attatggtgg aacagaatgt gtggatattg tcgaaacgct tgctattgaa cgtgctaaga
     8461 aacttttcgg tgccgcattt gccaatgttc aagcccattc cggaagtcaa gccaatgcag
     8521 cagcttatat ggccttaatt gaggctggtg ataccgtatt aggaatggat ttggctgcag
     8581 gtggtcatct cacgcatggc tctccagtaa atttttctgg aaaaacttac cattttgtgg
     8641 gttattcagt agatgctgat actgaaatgt taaattatga ggcgatttta gaacaagcaa
     8701 aggcggtcca gccaaagctt atcgttgcag gggcttcggc ctactctaga agcattgatt
     8761 ttgaaaagtt tcgtgccatt gctgatcacg taggtgctta cttaatggtg gacatggctc
     8821 acatcgctgg ccttgtagca gcaggtgtgc acccgagtcc tgtcccttat gcgcatattg
     8881 tgacgtcaac aacccataaa accttacgtg gtcctcgtgg aggcttgata ttaaccaatg
     8941 acgaagctct tgctaaaaag attaattcgg ccgttttccc tggtttacaa ggaggacctc
     9001 tggaacacgt gattgctgcc aaagcggttg ccttcaaaga agccttggat ccagccttta
     9061 aagactatgc acaagctatt attgataata ctgcggctat ggcagcagtt tttgcacaag
     9121 atgatcgttt ccgtctgatc tcaggtggta cagataatca tgtcttttta gtagatgtca
     9181 ctaaggttat tgctaatggt aaactagcgc aaaatcttct ggatgaggtt aatattaccc
     9241 ttaataaaaa tgccattcct tttgaaacct tgtcgccatt taaaacctca ggtattcgca
     9301 ttggttgtgc tgccataacc agccgtggta tgggtgttaa agagagtcag accattgccc
     9361 gtcttatcat caaagccttg gttaatcatg atcaagaaac tattcttgaa gaagtccgtc
     9421 aggaagtgcg tcagttaacc gatgctttcc cactctataa aaagtaagga atcatgttag
     9481 atagttatat aaaacgtatt gtcattcatc aattttctcc caatgacact gagttactgt
     9541 tatcagatcg tttagtgagc attacaccac gaattgatga atatttccgg aaaaaattgg
     9601 ctaaagtgtt ctcggatgaa gccaaacggg gtcagtttga ggctaataat acctttttta
     9661 caaccattgg tgatgatttg ttggaaacgt ctgttaccat tgcccagtta tggaaggaag
     9721 cctttgttat ttcagaggat caaaaaacca atgatttagt ctttgtccag tttgataagg
     9781 atggcgaacc cttttttgct ttcttgcgaa ttgctttgaa agaacagttt gctcaccttt
     9841 ctgacaatta tgaacatcca tttacggtga cgcagaataa tctgccgagt cccacccaga
     9901 caccagatga agcactagtc attaatctca aatctggtca gtactacttg attgaaaagc
     9961 gtgtgaagca taatggcagt tttgctaatt atttttcaga acacctcttg aaagttacac
    10021 ctgagcaatc tgttaaaaag tctatcaaaa tgatagaaca aaccgctcaa aaaattgcag
    10081 agcattttaa tcaagatgat tttacttttc agtcaaagat gaaatccact ctttttaagc
    10141 agttagaagc agacgacgtt ttatcaccag aaaaattagc tgaccaactc tttgatgata
    10201 atttaacagc ccgtttaacc tttgtggatc aagtcaaaga agtcattcca gaacctataa
    10261 aaattagcga tattgatcat tctagacaga taaaaaaact agaaaatcaa aagctatcgt
    10321 tatcaaacgg gatagaatta acagtaccaa atgccattta tcaggatgct gaagcggttg
    10381 aatttttgtt aaatgatgat gggacttatt caatcttgat taaaaatata gaggatatta
    10441 aaaccaaata gatgtttaga cttttaaagc gtgcctgcag ttttcttctt ctttttgtca
    10501 tttatcaatc ctttgttatt catcacaatg ttcaacgtgt tttggcatat aagcctatgg
    10561 ttgagaaaac attagcagaa aatgatacaa aggcaaatgt agacttagtc ttagctatga
    10621 tatacaccga gacaaaagga ggagaagcag atgtcatgca atccagtgaa agcagttcag
    10681 gacaaaaaaa ctcgattaca gatagtcagg ccagtattga gcacggggtg aatttactgt
    10741 ctcataatct tgctctagct gaggaagcag gagtagattc ttggacggct gttcaggcct
    10801 ataattttgg aacagcttat attgattata ttgctaaaca tggaggtcaa aataccgttg
    10861 atttagcgac tacctattcc aaaaccgttg tggcgccaag cctaggcaac actagtggtc
    10921 aaacctattt ttattaccac cccctcgcct tgatttctgg aggtaagctt tataagaatg
    10981 gcggcaatat ctattactca cgagaagttc atttcaatct ttatttaatt gaactgatga
    11041 gcctttttta gaaagttatc atgaaacgat tacgtccata tttgaaaggg tacctaaaag
    11101 aaagtatctt aggtcctctt tttaaattat tagaagcttt atttgaatta ttagtccctt
    11161 tgttaattgc taacatgatt gatatatcga ttagtcaaca caacagccag ggaattttga
    11221 gggttgtttt aacattattt ggtttagcaa ccattggctt attgctttcc gttacagccc
    11281 agtatttttc ttcgaaagca gctgttggtt ttacaagaca aatgacagat gatttgttta
    11341 aaaaaatcat gtttttgagc aaggaggacc aagaccatct tggttatgct agtctgttat
    11401 cacgattgac cagtgatagt tttcaaattc aaactggtat caatcaattt ttgcgtcttt
    11461 ttctaagggc ccctattatc gtatgtggtg ctacggtaat ggcttattgg attagtccaa
    11521 gtctaaccct atggtttgtg atgatggtga ttgtcttact aacacttgtt tttgttatgt
    11581 cacatttatt aggtcccctt tatctcttaa tccgccgaga aacagatcac ttggttcgtt
    11641 tgactagcca acagttacaa ggaatccgag taattaaggc ttttaatcaa acccaaaaag
    11701 aactccaagc atttaagcag caaaatatgc tcttaagtcg ccaccaatat caagctgcta
    11761 ccctagccaa tgtcttgaat cctatgactt ttttagtggt caatcttacc ctgttaatct
    11821 tgatttggca aggaagttgg caagtagctc atagatcgct tagtcagggc atgttggttg
    11881 ctttaattaa ttacctcttg cagattttgg cagaactttt gaaaatgacc atgttgatgg
    11941 gaaccatcaa tcaaagtgta acagccgcaa aacggattaa tcaggtgttt gttttagctg
    12001 atgaagcgcc tttgccctca cttaaagatg gtcctatttc aactcatctg ttgaccatcc
    12061 ggcacttgac ctttacttat ccaggagcag cagagccaag tctgtatgat attcagttaa
    12121 gtgctgacca gggagaatgg attggcatca ttggtggtac tggagcaggc aaaacgactt
    12181 tgattgactt gatctgccaa acttattcac catatagtgg tgagattagt cttaattggc
    12241 aaggagaagt gccaaaaaca ctaaccgagt ggcgaaatgt gattgctctt gttcctcaaa
    12301 aagctcaatt atttaaagga acgattagaa gtaacctcct actgggacaa tcaatgccta
    12361 ttagtgatga ggaactgtgg cgggctcttg aattggctca agcaaaagag tttgtcgctg
    12421 ccttaccaga acaattagag gcacctgttg aagccttcgg tcgtcatttt tcaggagggc
    12481 aacggcaacg tctagctatt gccagagcac ttctgaagcc aaaacccatt ttgattttgg
    12541 atgatgccag ttccgcctta gacaatgaga ctcgaggacg actttttaaa gctctaaaag
    12601 aagagttatc agatgtttta gtgattctag tcacgcaatc catcaagaat ttgcagtttg
    12661 ctgataagat tttagtgttg gaacagggcc atcaattgga ttttgccagt catgaccagt
    12721 taaaagtaag caatgcactt tatcaagaaa tgctagcgct ttacgtgaag ggggagcaaa
    12781 aatgattaaa acagatcatc acctcctaaa gcgaatgctt cacgatttgt taaaaaaacc
    12841 tctacctgtt tgtattctcg tgatagctag ttttgtacaa gttggtttaa gtgtttactt
    12901 gcctgttctt atcggtaaag ccgttgatat gagtttgtca gtcaattctt ggcaaacttt
    12961 aaaatggctg ttggggcaaa tgttagttgt tattgtggtc aacacattga ttcaatgggt
    13021 gatgcctctt gtctatagtc gtttgctata tcaatatagc cagcaattga aagataagct
    13081 tttggaaaaa attcatcgtc ttccttttgc atatctagat cgtcaaacca ttggagattt
    13141 ggttagtcga gtgataaccg acactgagca actgatcaat ggtctacaaa tggtttttaa
    13201 ccagtttata cttggactat tgacgattct gtgtaccatt atagctatgg cacagattga
    13261 ctggttcatg ttaatcttgg ttttggtgtt aacaccaagc tccctctttt tagctcgttt
    13321 tattgctcaa aagagttttc attatgcaca agcacagacc aaaagcagag gaaatttagc
    13381 tcagtttact gaagaaatcc ttcgtcaaga aggtttggtt caactgttta atgctcaaga
    13441 gcagtctatc tgtgattatc atgttttaaa taaaacctat tgcgaggctt cacaaaaagt
    13501 cattttttac gcctcaacag tcaatccagc cactcgcttt attaatagtg tcatttatgc
    13561 tttactggca ggtttagggg ctgttcgtat catggctgga cttttttcag taggtcagct
    13621 aaccactttt ttaaatgttg ttgtccaata taccaaacct ttcaacgata tttcatctgt
    13681 cttggcagag atacagagtt ctttagcttg tgcgcaaagg ctttatgacc ttttagatat
    13741 tgaaataaaa gaggaagagc actttttaac atttaaggca tcagcggtta aggggcaaat
    13801 tgactttgaa gaagtaagtt tttcttatca aaaagacagg cccttactga aggatattaa
    13861 tttttctgtt cctgcgggtt ccaaggtagc gattgtaggg cctacaggtg caggcaaatc
    13921 gactttaatc aatttactca tgcgctttta tgaacttgat gctggtagca tcaagttaga
    13981 taaagttcct attaagtgtt atgctaagga agaacttagg tccattactg gcatggtatt
    14041 gcaagaaacc tggttgaaag atgcgactgt tcatgagttg attgcttacg gcagtgaaga
    14101 ggctagccgt ggtgaagtag tggcagcagc caaagcagct catgtacact tctttattat
    14161 gcaacttcct aagacttatg atacttactt aagtgcttct gatgatgctt tgtcccaagg
    14221 gcagctccag ttattagcta ttgccagaat gtttttgaaa aaaccaaaag tcttgattct
    14281 agatgaagcc acctcctcta ttgatattag aacagaagct gttattcaag aggcactaaa
    14341 agaacggacc agctttatca ttgcccatcg tttatcaacg attcaatcag ctgatttgat
    14401 tcttgttatg gatcaaggtc gattggttga gtggggaaca catgccagct taatgtcaaa
    14461 aaacggatgt tatgctagat tacaaaagat agaataaagt tcgtctttta acaaaggtga
    14521 actttttaac tcttcctaaa gttagttaat taacaaactt taaactgtta tataaaaaaa
    14581 taacagcgaa aaagcggacg atcttttttt ataagtgaga aaaaacagtt gactaaagaa
    14641 gaaataactc aaaaaagtga gcaatatatt tggaagcgtt ttttgactgt ggtataatag
    14701 agccctatat aatattgtga aatgaggaac aaaatatatg agtaaaatcg ttgttgttgg
    14761 tgcaaaccat gctggtacgg cctgtatcaa gactatgtta acaaactacg gtgatgctaa
    14821 tgagattgtt gtatttgacc aaaactcaaa tatttcattt ttaggctgtg gtatggcact
    14881 ttggattggt gagcaaattg ctggaccaga aggacttttc tattcagata aagaagaatt
    14941 agagtcttta ggggctaaag tttacatgga atcacctgtt caatcaatcg actacgatgc
    15001 caaaacagtg acagcgcttg ttgatggtaa aaaccacgtg gaaacttacg acaagttgat
    15061 ttttgcaact ggctcacaac ctatcttacc accgattaaa ggtgccgaaa tcaaagaagg
    15121 atcacttgaa tttgaagcaa ctcttgaaaa tcttcaattt gtgaagttat accaaaactc
    15181 agctgatgtg attgcaaagc ttgaaaacaa agacattaaa cgtgtagccg tagttggtgc
    15241 tggttacatt ggtgttgagt tagctgaagc tttccaacgc aaaggcaaag aagtggttct
    15301 gattgatgtc gtggacactt gcttggcagg ttattacgac cgtgatttga ctgacttaat
    15361 ggctaaaaac atggaagaac atggtattca actagccttt ggtgaaacag ttaaagaagt
    15421 agctggtaat ggtaaggttg aaaagatcat tactgacaaa aatgagtacg atgtggatat
    15481 ggttatcctc gccgttggtt tccgtccaaa cacaactctt ggaaatggta agattgatct
    15541 tttccgtaat ggtgctttcc ttgtcaataa acgccaagaa acttctattc caggtgttta
    15601 tgctatcggt gactgtgcaa ctatctacga taatgctact cgcgatacaa actacattgc
    15661 tttagcttca aatgctgtcc gtacaggaat tgtagcagca cataatgctt gtggtacaga
    15721 ccttgaagga atcggtgtcc aaggctcaaa cggtatttcc atttacggct tgcacatggt
    15781 atcaactggt ttgactcttg aaaaagctaa acgacttggt tttgatgctg cagtgactga
    15841 gtatactgat aaccaaaaac ctgaatttat cgaacacggt aacttcccag taaccattaa
    15901 gattgtttac gataaagact cacgtcgtat cttgggtgct caaatggcag cccgtgaaga
    15961 tgtgtcaatg ggaattcaca tgttctcact tgctatccaa gaaggcgtaa ccattgaaaa
    16021 gttggcatta actgatattt tcttcttacc acatttcaac aaaccttaca actacatcac
    16081 aatggcagca cttggtgcca aagactagta tctgatagga aaaagccaga gcagttcagc
    16141 agcactggct tttttgcttt ttaattgttg caacctgaaa agttctaaac ttatgattat
    16201 cctcttatag cacgttggaa aaggaatatc cggcaaaaca aaaagagacc aaagtctctt
    16261 ttgtgattag tttttagcag cagaagcaaa ttcttcctta gcaaaggctt catcaatgat
    16321 agcttttaat tgatttgcag aggcttgcat tttttgcaat tcagcatcgt taagtggaat
    16381 gtttactgga cgaacaattc catacgctcc aacgatagct ggttgaccaa tatagcaatc
    16441 ttcaacacct tcgtattggc cttcttggaa aacagaaagt ggaagaacag cattttcatc
    16501 atcaagaata gcttttgtaa tacgagctag ggcaactgca ataccgtaga aagtagcacc
    16561 ttttttattg atgattgagt aagcagcatc acgtacagaa atgaaaagat caactaggcc
    16621 ttgctcatca atgtcacgat tagcttggag ccagtcgtaa agacctacac cagcaacgtt
    16681 agcgtgtgac caaacagcaa attcagagtc accgtgttct cccatgatgt aagcgtgaac
    16741 cgaacgcgca tcaacaccga ttttagcagc cagagcttga cggaaacggg ctgaatctag
    16801 agatgtacct gatccaataa cacgctcttt agggaaacct gagaatttcc atgttgagta
    16861 agtcaaaacg tcaactgggt tagcagcaac aagaaagatt cctttgaaac cagatgcaac
    16921 gatttgtgta acaacctctt tattgatacg aaggtttttt tcaacaaggt caaggcgtgt
    16981 ttcacctggt ttttgaggag caccagctgt taagacaaca agatcagcgt catgacagtc
    17041 tgagtagtca gctgcataga tttttttagg tgatgtaaaa gctagagcgt ggcttaaatc
    17101 ttctgcatct ccttgagttt tttctttaaa aatatcaata ataccaagtt cttgagcaat
    17161 attttgtgtt accaatgcaa aagcgtatga tgaaccgacg gcaccatcac cgaccaagat
    17221 tacttttttg tgttgtttag ttgcagtcat ttctaaacat ctccttaatt ttattagagc
    17281 aaaagctcat acggtcttat tttaacatat tgataattgt ttgtcattga attcagaaaa
    17341 ataatgaaaa tagtttcctg ataacgcttc caataagaaa aaataaggaa tttatggtat
    17401 aatgaaatct agtattgact agaaaaggag cattccttaa atgcaagatc gaaatttaat
    17461 tgacgtcaat ctgacaagtg aaatgaaaac gagtttcatt gactatgcca tgagtgtcat
    17521 tgtggcaaga gctttgccag atgtgcgtga tggtctgaaa cctgtgcatc gtcgtattct
    17581 ctatgggatg aatgaactcg gtgtgacacc tgataaaccg cataaaaaat ctgctcgtat
    17641 tacgggtgat gtcatgggta aatatcaccc gcatggggat tcttctattt atgaagctat
    17701 ggttcgtatg gctcagtggt ggagttatcg ccatatgctt gttgatggcc atggcaattt
    17761 tggttctatg gatggtgatg gtgctgccgc tcaacgttat actgaagcgc gcatgagtaa
    17821 aattgcgctt gaattattaa gggatatcaa taaaaatacc gttaattttc aagataacta
    17881 cgatggaagc gaaagagaac cagtagtatt accagctcgc tttcctaatt tattagtcaa
    17941 tggagcgaca gggattgccg ttgggatggc aactaacatt ccgcctcata atttggcaga
    18001 gtctattgat gctgtcaaga tggtaatgga acatccagac tgtacaacaa gagagttgat
    18061 ggaagtgatc cctggacctg actttccgac tggtgccctt gttatggggc gctcaggtat
    18121 tcatagggct tatgatactg gtaaaggttc tatcgtttta cgctctcgaa cagaaattga
    18181 aacaactcaa acaggtcggg aacgtattgt ggttactgaa tttccctatg gcgtcaataa
    18241 aacgaaagtg catgaacaca ttgttcgttt agcacaagaa aaaagactag aaggtattac
    18301 agcagttcgt gacgaatcta gccgtgaagg agtgcgtttt gttatcgaga ttcgtcgaga
    18361 ggcatccgcg acagttatct taaataacct ctttaaatta acaagccttc agacgaattt
    18421 cagttttaat atgttggcta ttgaaaatgg tgtgcctaaa atattgtcac tcagacaaat
    18481 cattgataat tacattagtc atcaaaaaga agttattata cgtcgcaccc gatttgataa
    18541 agataaagct gaagccagag ctcatatttt agaaggctta ctgattgccc ttgatcattt
    18601 agacgaagtg attgctatta ttcgtaacag cgagacagat gtcattgctc agacagaatt
    18661 gatgtcacgt ttcgacttgt ctgaacgcca aagtcaagct attttggata tgcgtttgcg
    18721 tcgtttgact ggtttggaac gtgataagat tcaatcagaa tatgatgatt tgttggcctt
    18781 gattgctgac ttatctgata ttttagccaa accagaacgt attatcacta tcatcaaaga
    18841 agaaatggat gagatcaagc gtaaatacgc taacccacgc cgtacagaat taatggtcgg
    18901 tgaagtcctt tctctagaag atgaagacct aattgaagaa gaagatgttt tgattacctt
    18961 gtcaaataaa ggttatatca agcgtcttgc tcaagatgaa tttcgtgccc agaagcgtgg
    19021 tggacgtggc gtccaaggaa ctggggtcaa caatgacgac tttgtccgtg aattggtatc
    19081 aacaagtacc catgatactt tgctcttctt tacgaatttt gggcgtgtct ataggttgaa
    19141 agcttacgaa attccagaat atggtcgtac agcaaaaggt cttccaattg ttaatctatt
    19201 gaaattagaa gatggtgaaa ccattcaaac tattatcaac gcacgtaagg aagaaacagc
    19261 aggtaaatcc ttcttcttta caacgaaaca aggcattgtc aaacgtacag aagtttcaga
    19321 atttaataat atccgtcaaa acggtctccg tgctcttaag ttgaaagagg gagatcagct
    19381 gattaacgtc cttttgacaa gcggtcagga tgacattatc attggaactc attccggata
    19441 ttctgttcgt tttaatgaag caagcatccg caacatgggc cgatcagcta cgggtgttcg
    19501 cggggttaag cttcgtgaag atgatcgcgt tgttggcgcg tcacgtattc aagataacca
    19561 agaagttctt gtaattaccg aaaatggctt tggtaagaga acaagtgcta cagactatcc
    19621 tacaaaaggt agggggggaa aaggaataaa aacagctaat attaccccta aaaatggtca
    19681 attagctgga ttggttaccg tagatggtac agaagatatc atggtgatta caaataaagg
    19741 tgtcattatt cgcacaaatg ttgcaaatat ttcgcaaaca ggtcgtgcta ctttgggtgt
    19801 taaaattatg aaactagatg ctgatgctaa gattgtaaca tttaccttgg ttcagcctga
    19861 agacagtagt atcgatgaaa tcaatactga tcgtgaaaat agtatttcaa aaaataaaga
    19921 taattaatca taacaaggag taaaaaatgg taaaaaaaca aaagcgtcga aaaataaaat
    19981 ccatgtcttg ggcaagaaaa ctactaatag ctgtactttt aattctaggt ttagcattgc
    20041 tttttaacaa acctatccga aatacattaa ttgctcgaaa ctctaataaa tatcaagtaa
    20101 cgaaagtttc gaaaaaacaa atcaaaaaga ataaagaggc taagtcaaca tttgatttcc
    20161 aagccgtaga gccagttagt acagagagtg tcttgcaagc acaaatggcg gctcagcaac
    20221 ttcctgttat agggggcatt gccataccag agcttggcat taatttacca atttttaaag
    20281 gtttaggaaa tactgagctt atttatggcg caggaacgat gaaagaagaa caagttatgg
    20341 gaggagaaaa taattattct cttgccagtc atcatatttt tggaattaca ggttcatctc
    20401 aaatgctctt ttcgccgctt gaaagagcac aaaatgggat gtccatctat ttaacagata
    20461 aagaaaaaat ttacgaatac atcataaaag atgttttcac ggtagctcct gaacgcgttg
    20521 atgttatcga tgatacagct ggtctcaaag aagtgacttt agtgacttgt acagatatcg
    20581 aagcaacaga acgtattatt gtcaaaggag aactaaaaac agaatacgat tttgataaag
    20641 cgcc
//
LOCUS       DAUCWO010000029        20634 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUCWO010000029 DAUCWO010000000
VERSION     DAUCWO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.029"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            542..931
                     /locus_tag="VGY20_000996"
     CDS             542..931
                     /locus_tag="VGY20_000996"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ4273860.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1025..2077
                     /locus_tag="VGY20_000997"
     CDS             1025..2077
                     /locus_tag="VGY20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ4273861.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGAAGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2216..3748
                     /locus_tag="VGY20_000998"
     CDS             2216..3748
                     /locus_tag="VGY20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273862.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVLPENTNKQELGILMAGGSIYKEEG
                     HV"
     gene            3741..4805
                     /locus_tag="VGY20_000999"
     CDS             3741..4805
                     /locus_tag="VGY20_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273863.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMVAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4807..5763
                     /locus_tag="VGY20_001000"
     CDS             4807..5763
                     /locus_tag="VGY20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4273864.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5976..7694)
                     /locus_tag="VGY20_001001"
     CDS             complement(5976..7694)
                     /locus_tag="VGY20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ4273865.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMNKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(7870..8439)
                     /locus_tag="VGY20_001002"
     CDS             complement(7870..8439)
                     /locus_tag="VGY20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4273866.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEIIISAIITFVLIPTLSRLKR"
     gene            complement(8497..9042)
                     /gene="coaC"
                     /locus_tag="VGY20_001003"
     CDS             complement(8497..9042)
                     /gene="coaC"
                     /locus_tag="VGY20_001003"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ4273867.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     LVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(9035..9727)
                     /gene="coaB"
                     /locus_tag="VGY20_001004"
     CDS             complement(9035..9727)
                     /gene="coaB"
                     /locus_tag="VGY20_001004"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ4273868.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKRLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            9908..10759
                     /locus_tag="VGY20_001005"
     CDS             9908..10759
                     /locus_tag="VGY20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ4273869.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            10756..11955
                     /locus_tag="VGY20_001006"
     CDS             10756..11955
                     /locus_tag="VGY20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ4273870.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            11952..12950
                     /locus_tag="VGY20_001007"
     CDS             11952..12950
                     /locus_tag="VGY20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ4273871.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            12992..13324
                     /locus_tag="VGY20_001008"
     CDS             12992..13324
                     /locus_tag="VGY20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ4273872.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            13317..14129
                     /locus_tag="VGY20_001009"
     CDS             13317..14129
                     /locus_tag="VGY20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ4273873.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            14122..15003
                     /locus_tag="VGY20_001010"
     CDS             14122..15003
                     /locus_tag="VGY20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ4273874.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            15050..16069
                     /locus_tag="VGY20_001011"
     CDS             15050..16069
                     /locus_tag="VGY20_001011"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ4273875.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     YLIFS"
     gene            16197..17867
                     /locus_tag="VGY20_001012"
     CDS             16197..17867
                     /locus_tag="VGY20_001012"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ4273876.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            17952..19529
                     /gene="cls"
                     /locus_tag="VGY20_001013"
     CDS             17952..19529
                     /gene="cls"
                     /locus_tag="VGY20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ4273877.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGRWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLKQRP
                     WYQKLIGLLVRTIAPLL"
     gene            19869..20207
                     /locus_tag="VGY20_001014"
     CDS             19869..20207
                     /locus_tag="VGY20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273878.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         6323 a         3488 c         4268 g         6555 t
ORIGIN      
        1 acctttttta atgtatttta actgaaaaag gctttcataa ttttaaaaaa atgttaaaat
       61 aggtgtataa caactgatta ccaagagatt attatcatta tcttctaagg tgtcattgtt
      121 atggtgacaa tcaaccatac gctactaaag ataggtagcg ttcgatcaca ggttgatcaa
      181 gtgattgaaa aatcaatatt tataatttta caaatatctg tatcaatcca tgttgaatag
      241 tctatgcttc taaggtgtcg acgttaatgt actgacggga ccgttatagt ctttcttttt
      301 ggagaaacgt cggatgccag tgaactagtt gaagttaaag atgttaattc aaacagtgct
      361 gaagagattg atatcgtttt gaacggtcgt ttacttcaaa gcaaagaaat taccagtcta
      421 tcggagatga tataagtgtt aaagttagat cacgttaatt atctatcaag tcgggtgcat
      481 atttcgttaa agtatccaca ggggtttcaa tagatgtagc tattatgaaa ggtgatgttc
      541 aatggggact actgatttag ttagttgtgc tgtacaagct agtgagtatg cttatgtccc
      601 ttactctcat tttccagtag gagcagcttt aaaaacaaaa gatggaacga tctatactgg
      661 ctgtaatatt gaaaatgtta gttttggtct gacaaattgt ggagagcgaa cagctatatt
      721 taaagccata tctgatggtc ataaagaatt ggttgaaatt gctatttatg gagaaactat
      781 gcaacctgta tctccttgtg gggcttgtcg tcaggtaatg gcagaatttt ttgctccttc
      841 ttctttagtc actttaattg ctaaaaatgg tcaaactgtt gaaatgactg ttggagattt
      901 actcccctat tcttttacag atttaatata atctgcttta atttctgtta tctagccgtc
      961 ttggctttat atttactttc agcaacttat ttgctagaaa tcttttttag gagggttcat
     1021 attcatgaac aagaaattta ttggtcttgg tttagcgtca gtggctgtgc tgagtttagc
     1081 tgcttgtggt aatcgtggtg cttctaaagg tggggcagca ggaaaaactg atttaaaagt
     1141 cgcaatggtt accgatactg gtggtgtaga tgacaaatca ttcaaccaat cagcatggga
     1201 aggcctgcaa tcttggggta aagaaatggg ccttcaaaaa ggaacaggtt tcgattattt
     1261 tcaatctaca agtgaatctg agtatgcaac taatcttgat acagcagttt caggagggta
     1321 tcaactgatt tatggtatcg gctttgcatt gaaagatgct attgctaaag cagctggaga
     1381 taatgaagga gttaagtttg ttattatcga tgatattatc gaaggaaaag ataatgtagc
     1441 cagtgttacc tttgctgacc atgaagctgc ttatcttgca ggaattgcag ctgcaaaaac
     1501 aacaaaaaca aaaacagttg gtttcgtggg cggtatggaa ggaactgtca taactcgatt
     1561 tgaaaaaggt tttgaagcag gagttaagtc tgttgacgat acaatccaag ttaaagttga
     1621 ttatgctgga tcatttggtg acgctgcaaa aggaaaaaca atcgcagcag ctcagtatgc
     1681 agcaggtgct gatgttattt accaggcagc aggaggcact ggagcaggtg tatttaatga
     1741 agcaaaagct attaatgaaa aacgtagtga agctgataaa gtttgggtta ttggtgttga
     1801 ccgtgatcaa aaagacgaag gaaaatacac ttctaaagat ggcaaagaag caaactttgt
     1861 acttgcatca tcaatcaaag aagttggtaa agctgttcag ttaatcaaca agcaagtagc
     1921 agataaaaaa ttccctggag gaaaaacaac tgtctatggt ctaaaagatg gcggtgttga
     1981 aatcgcaact acaaatgttt caaaagaagc tgttaaagct attaaagaag cgaaagcaaa
     2041 aattaaatct ggtgacatta aagttcctga aaaatagtgt ttaagaacaa gaagcgcgac
     2101 ctttgtcggt cgctcttttt aaactgagat aatttttatc tcagtaaagt ttgtgatagg
     2161 tgatagatca taggctttac tgaaataaaa gattgcattg aaaggaaatg atcctatgac
     2221 acagcatgtc attgaaatga gagaaatcac gaaaaaattt ggtgattttg ttgcaaatga
     2281 ccacattaac ttaaatgtca gaaaaggtga gattcatgcc ttattgggtg aaaatggggc
     2341 aggtaaatca actttgatga atatgctagc tggtctctta gagcctacta gtggagaaat
     2401 tgtcattaat gacaagccag ttcaaattga ttcgccatct aaatcagcta agcttggtat
     2461 cggaatggta catcaacatt tcatgttagt agaagccttc acggttgctg agaatattat
     2521 tttaggaaat gaagtagtaa aaaatgggtg cttagatctt aaccaagcat cgaaagatat
     2581 taaggtacta tccgaaaaat atggccttgc aatcaatcca tcagctaaag tttctgatat
     2641 ttcagtagga gcacaacaac gtgttgagat tttgaaaacc ttgtatcgtg gtgcagatat
     2701 tcttatcttt gatgagccaa ctgccgtttt aacacctgca gaaattaaag aattgatgac
     2761 tatcatgaag aatctcgtga aagaaggaaa atcaattatt ttaattaccc ataaactgga
     2821 cgagattcgt gctgtcgccg accgtgtcac agttattcgt cgtggtaaga gtatcgaaac
     2881 agttgatgtg gcaggagcaa cttcacaaga tttagctgag atgatggtgg gacgctcggt
     2941 ttcctttaca actagcaaaa aagctgctga accaaaagat gtggtcttat ccatcaaaaa
     3001 tcttgaagta gatgaaaata gagggattcc agcagttaag gggctatctc tagatgtccg
     3061 agctggtgaa attgtaggga ttgctggaat tgacggaaat ggtcaatctg agttaattca
     3121 agctattaca ggtttacgta aagtcaaatc tggaagtatc atgattaaaa acaatgaagt
     3181 cactcatttg tcttcacgca aaattactga gttatcagtg ggtcacgtgc cagaagatcg
     3241 tcatcgagat ggtttgatac tagatctttc tttagctgaa aatacagcct tacaaaccta
     3301 ttacaaacaa cctctaagtc aaaatggaat cttaaattat actaaaatta atgactatgc
     3361 tcgtcaatta atgaaagaat ttgatgttcg cggagccaat gagctagtac cagctagagg
     3421 attttctggt ggtaaccaac aaaaggccat tattgctcgt gaagtggacc gtgatccaga
     3481 tttacttatt gttagccaac caacacgtgg tcttgacgta ggagctattg aatatatcca
     3541 taagcgtttg attaaagaac gtgataaagg taaagctgtc ctcgttgtta gttttgaact
     3601 tgatgaaata ttgaatttat ctgatcgaat tgctgttatt catgatggta aaattcaagg
     3661 tatcgtattg ccagaaaata ccaataaaca agaattgggt attttaatgg caggtggttc
     3721 aatttataag gaggaaggtc atgtctaaaa atgcgcaaaa aattgctgta ccattgattt
     3781 ctgtcttgct aggattttta cttggcgcta ttattatggt tatctttggt tatgatccta
     3841 tctgggggta tgaaggttta ttccaaattg cctttggtag tgttaaaaat attggagaaa
     3901 tattccgttc aatggggcca ttgattttaa tagcgcttgg ctttactgtt gctagccgtg
     3961 caggcttctt taacgttggt ttgtctggac aggctttagc tggatggatt tcagcaggtt
     4021 ggtttgcact cttaaatcca gatatgcctc gtccattatt gattcttatg acagctctta
     4081 tcggaatggt tgctggtggt attgctggag ctattccagg gatacttcgt gcttatcttg
     4141 gtacaagtga agttatcgtt acgattatga tgaactacat cattttatat gtcggaaatg
     4201 ctattgttca acgtggttat cctgagagtg tcaaacaaag tattgattca actattcaag
     4261 ttagtgataa tgctagttat caaacgcatt ggttatcagc cctaactaat aactctcgta
     4321 ttaatattgg tattttcttt gccattattg ccattgccct aatctggttc ttgcttaaca
     4381 aaactacttt aggttttgag attcgttcag ttggtctcaa cccacatgct tcagaatacg
     4441 cagggatgtc atcaaaacgt actatcattt tatcaatgat tatctctgga gctcttgcag
     4501 gtcttggagg agtagttgaa ggtctaggaa cctttgaaaa tgtttttgta caaggatctt
     4561 ctttagcagt tggttttgac ggtatggcag ttagcttatt agcagcaaat tcaccgcttg
     4621 gaatattttt ctcatcattc ctatttggtg tcttaaatat tggtgctcca ggaatgaata
     4681 ttgcaggcat tccaccagaa cttgttaaag tagtaactgc atcaatcatt ttctttgttg
     4741 gttcacatta cctcattgaa cgttacatta ttcgccctaa aaaattagtg aaaggtggta
     4801 aataagatga gtttagtaac aatttttgcc ttattggtgt catctatgct tatttatgca
     4861 acaccactca ttttcacaag tattggagga actttctcag agcgctcagg cgttgttaat
     4921 gttggtttag aaggtatcat ggtcatggga gcgttctctg ggattgtttt caacttagag
     4981 tttgctgaaa catttggaaa agcaacgcct tggatagccg ttttagtcgg tggtattgtt
     5041 ggtttgattt tttctttgat tcacgctgta gcaaccatca actttcgagc tgaccacatt
     5101 gtcagtggta cagtgttgaa cttgttagca ccttcttttg ctgtcttctt ggttaaagct
     5161 atgtacggta agggacaaac agacaacatt caacaatctt ttgggaagtt tgatttccca
     5221 ggtttatcac aaattcctgt gattggtgat attttcttta aaaatactag ccttattggt
     5281 tacttcgcca ttgctttttc gttctttgct tggtttttgc tttataaaac caggtttggt
     5341 ctgcgtttac gatcagttgg ggaacaccct caggcagctg acacacttgg tattaacgtc
     5401 tatttgatga aatactatgg tgtgatgatc tcaggttttc ttggtggaat aggtggagct
     5461 gtttatgctc agtcaatctc cgttaacttt gctgtaacaa ctatcttagg tcctggattt
     5521 attgctttgg cagctatgat ctttggtaaa tggaatccag ttggcgccat gctatctagt
     5581 cttttctttg gcttatcgca aagtttagcc gttattggtg ctcaattacc attactagaa
     5641 aagattccaa cggtttactt acagattgca ccttatatgg taacgattat tattttagct
     5701 gctttctttg gtcaagcagt tgcaccaaaa gctgatggaa tcaactatat taaatctaaa
     5761 taacgttata ttagtaagcc tcttagtgag gtttaacagc actcctaaag caagatagtt
     5821 aggtctagct ttaagggtgc ttttttggga cttaccgcaa gtttggattg ccagtcacca
     5881 tcacaataga agcaagaata gactaaaaag aactaagtca gcggcttagt tctttttgct
     5941 tgtctttcgt ttttactgat agctcttaag ttgttttagc caacaaaggt attgatttct
     6001 gtttcaatgt tagcaatttt ttcttgagcg atgtcaagag tatcacctat tgtggcaata
     6061 tagaacttga tttttggttc agttcctgaa gggcgaactg caatccatga atcatcagct
     6121 aacgtgtact tcaaaacatt acttggagga gttgttaagt tagatattgt tccatcattt
     6181 ttagtagcgg tttgtttttg gaaatcttct aataatacaa tatcagtatt gttaaattgt
     6241 tttggaccat tttcacggaa tttgttcata atcttcttaa tttcagcagc tccatccaca
     6301 cctgaaagtg ttactgaaat cgttttttca gcaaaatagc catattcttt gtaaatttca
     6361 tcaataccat ctgcaagtgt taaaccacgc gaacggtaat aagctgcgat ctcagcaacc
     6421 aaaagaactg cttgaatagc gtctttatca cggacaaatg gtttaataag atatccgaag
     6481 ctttcttcaa aaccaaacat ataggtgtgg ttatgttttt cttcaaactc ttgaattttc
     6541 tcagcgatga atttgaatcc agttaaaacg ttaaacattg tagcgccata actttcggca
     6601 atttttgtta ctaattctgt tgatacgatt gatttagcga gagcagcatt ttctggaagt
     6661 gttcctgctg ttttatgagc ttccaggatg tatttcgcaa taatcgctcc aatttgattt
     6721 ccagaaagat ttttataaga accatctggt tggcgaattt caacgcctag gcggtctgca
     6781 tcaggatcgg tagctactaa gacatctgca tcaacttctc gtccgagttc ttcagcaaga
     6841 gcaaaggcag cttggctttc agggttaggc gatttaacag tagaaaagtc tggatctgcc
     6901 ttagcttgtg attctactac aacaacagat tcaaaaccgg cttgagcaag ggcacggcgt
     6961 gtcaacatct caccagttcc gtggagagga gtatagacaa ttttcatgtc ttttccaaag
     7021 ttattgatta aatcttgatt gatattaaca tctttaacct ctctaagata ttccatatca
     7081 acatcttcac cgataacttg aataagacct gctgatttgc tttcatctaa atcagcaagt
     7141 tcaactgcaa atggattttc aatagcacgg ataaagtctg ttagtgcatc agcatcagct
     7201 ggtggtagtt gacctccgtc ttgtccatag actttataac cattgaaagg tgctggattg
     7261 tggcttgcag taaccataat accagcatag gcatttaaat ggcgaacagc aaaggagagt
     7321 tctggtgttg gacgaagagc ttcaaatacg taagatttaa ttccatgctg tgccaagacc
     7381 tgtgcagact caaaagcaaa ttctggagag aagtggcgcg aatcataagc gatggctaca
     7441 ccacgttttt tagcttcctc tcccttagtt tctattaatt ttgctaatcc ttcggttgct
     7501 tgacggacaa cataaatatt aatacgattt gtaccagcac caatataacc acgcatacca
     7561 gcagtaccaa attctaagtt tgtgtaaaaa gcatcttctt ttgttttttc atccattgat
     7621 agtaattctt gacgaagata gtcaggaagc tgctcaaaat ctaaccattt ttgaaaattt
     7681 tctgtgtaag tcattgaaat actccttcaa tatttataaa cgctttcatt atatcatttt
     7741 gaagggaaaa aaacactaat tatttgcagc tgtttacaaa acttttttca tatttttaag
     7801 tagctaatgt aagaaagtta aaataaccaa gtttttaaaa gagaaaaact cggttattgt
     7861 gaaagctaat taccttttta gtcttgatag tgttggaata agtacaaagg taataatagc
     7921 tgagatgatt atctcaacaa tagcgtttga tgagatgatg gctgttagta gtgctttaat
     7981 atttccatca aaaactgtcg cgaaaaaaat aaatatccca gataatacaa atatagtatt
     8041 tgttagagaa ccaatgatac cggatactat taaaccaaac tgattaggaa tcaatttata
     8101 gcaataataa ggggtaatac caatcaaaat acgtgggata atagcaatta ttaaagaagc
     8161 gaaagttcca tgatcgacaa aaggtgaaaa gagataattt gtaggcagta gaatgatggt
     8221 atttgtaatg acacttataa tccccattaa tcctcctaaa atagcaccta tccttgggcc
     8281 atataagaca gaagcaataa taactgggat atggacaagc gttggtttga ttggaatagg
     8341 ccaaatatta aagacaagac tgctcacaaa atgaatgact aacatgatgg caaaaaagat
     8401 agcaatacga gaaatatctg ctgatttacg gtgattcatt ttactctcca aaaaatataa
     8461 tacttaattc taaaaaggtg atcagaaaaa cgactgttaa agtttccaaa tcgtatcaat
     8521 agtagctaag ataacatcaa tatcagccaa agcaccaggt cctttatctc cacaggctaa
     8581 taagctagat tttggaggaa tttctgtaaa ccctattgtc gataagcgtt taatattttc
     8641 ttgagtaata gggttttgat acattttggt attcatggct ggggctatta attttggtgt
     8701 ggtagctggt agggcaagag caacacttgt tacaagatta tcagcgaatc catatgctag
     8761 atgagcaata gtattagcag aggcaggtgc aacaataaat aaatctgtgc gttttgctaa
     8821 ttcgatatgg ttgataactt ttgggtcatg ctcatccata acatctaaat gaatagcatt
     8881 ttttgataac acttgcaggg ttaatggtgt aataaattgt gtagcagcct gagtcattat
     8941 gatgtgaaca tcatagccaa ttttagttaa ttgactcgta agatctgctg ctttataggc
     9001 agatatacta ccagaaacag ctaaggtaat atgtttagtc atgtttggtc atcctttcat
     9061 ataataagtc agcaattgcc tctttggtat ctgcagatgc aacttcgtta ttggatatca
     9121 aaagagcctt gtgcatacct gtttggatat caactaaatc attggcgaga atataatcag
     9181 catggttttt agctagactt gctctggcta ctttaattaa ctcttcttga ggcacattaa
     9241 ctagaagttt gaagccgact agtttaatat taggattcca ctgtttaaca taagagataa
     9301 ctttgggtgt ttttttcaaa aaaagaacct gataatcaga tgcagatgag attttcggct
     9361 cactgttatg ttcacataga aaacaattaa ggttatcagc ttgagagact tgttccaaat
     9421 cagtcatgta gactggagta tagtcagaaa cagccatact atgaattaga atgtcatggt
     9481 gaggaacctg atctttgagt gcagccatta agtcgttaac tgtttccact tcaataatcc
     9541 ttaggcgctt atttggcagg ggtttagtgg ctgttttagt agtgactagg gtaacgtcat
     9601 gatgatattg taaaaatcgc tctgtgatta attttccaag ttgcccagtt gagtgattag
     9661 tgatacctcg tactgcatct ataggttcag ttgttccacc tgatgttatt attaatttca
     9721 tagtcatatt ttatcaaaaa aggagctgat agtaaacgga agataatgac gccagacatt
     9781 gtcttgcgtt tgtattaata tggaatagta atagatttct agtaattaaa acgaagttct
     9841 ggaaagcaag acactatagt agctaaaatc taaattttaa ggtaaaatag agcaagcggt
     9901 ttcaagtgtg aaaggaagaa ttgtgataac tattagagat ttagcgtcac tgccgatttt
     9961 tgtttacggt gatggaaata aaaaagttcc aggagctttg agtccttttg tgtggacaga
    10021 ggtgttttta aaaactatca atcaagaacc taatcaactg atcttacata tttggccgat
    10081 gacaagaaca gttatattag gaatgttaga tagacagctt ccctactttg agctggctaa
    10141 aacagagatt ggcaataatg gttatgttcc tgttactcgt aatattggcg gtttggctgt
    10201 tgtcgctgat gatgggattt tgaatttttc gcttgttatt ccagaccatt tttcggaatc
    10261 tatcagtatt agtaatgctt atttaataat ggttgatgtc attagagaga gtttttcgga
    10321 ttattatcag cgtattgaat atcacgaaat taaaaattct tattgccctg gtaattttga
    10381 tcttagtatt gcaggccgca agtttgcagg tatcgcccag cgtcgtatca aaaagggaat
    10441 tgttgtgtct atttacttga gcgtttgtgg ggatcaagca gcgcgtggtc aactcatcaa
    10501 ggatttttat gaggccggaa cacaaggaga agttaccaag gttaattacc ctcaaattga
    10561 tccagaatgc atggcaactc tttcagaact ccttgagacg ccttttacag tggcagaagt
    10621 ccttgaacgt cttcgattga ccttgcgaca gcttggcttt tccctcacgg aaaaaagtcc
    10681 agatcaagcc ttactgacta attttgatgc tgtctatgaa cggatgcagc tagaagtcgt
    10741 cagaaaggaa ggtaaatgaa agaaagattt agtcctttat ttgagccttt gactttgcca
    10801 aatgggagtc aattggataa tcgctttgtt ttgtctccta tggtgaccaa ctcatccaca
    10861 aaagacggtt atgtcaccca agatgatgta tcctatgctt tgagacgagc agcttctgcc
    10921 cctctacaaa ttacaggagc ggcctatgtg gatccttatg gtcaattgtt tgaatatggt
    10981 tttagtgtta caaaagatgc cgatatttct ggcttgaaag agctagctca agcgatgaag
    11041 gcaaagggag ctaaagctgt cttacaactg acccacgctg gccggtttgc tagccatgcc
    11101 ttgactaaat atggctttgt ctatgggcct agttacatgc agttgagatc tccccaacct
    11161 catgaagtca aacctttaac aggtcagcaa atagaggaat tgattgcagc ttacgctcag
    11221 gccactcggc gggcaattca agcaggcttt gatggcgtgg aagtatcttc ggcgcagcgc
    11281 ttattgattc aaacgttctt ttcgactttt tcgaacaaga ggacagatag ttatggttgt
    11341 caaacacttt ttaatcgctc taagttaact ctagctgttt tgcaagctgt tcaacaggtg
    11401 attaaacaag aagctcctga tgggtttatt tttggatttc gtgctacacc agaagaaact
    11461 aggggaaatg atattggtta tagtattgac gagtttttgc agttgatgga ttgggtactt
    11521 aatgttgcaa aacttgatta tttagcgata gcaagttggg gccgtcatgt atttcgaaat
    11581 actgttcgtt ctcctggccc ttattatgga aggcgagtta atcaggtagt tagagattat
    11641 ttaagaaata agctacctgt tatggctaca gggggaatga acacacctga caaagctatt
    11701 gaagcattag ctcatgctga ttttattgga gtatctacac cttttgtcgt ggatccagaa
    11761 tttgctcata aaatcaaaga agggtgtgaa gagagtattc atttaaggat tcgacctgct
    11821 gatttgaaaa gtttagcgat tcctcaggct tctttcaaag atattgtacc tttgatggac
    11881 tacggggaat cattgcctaa agaatcaaga actttatttc gtagtcttac tcacaattat
    11941 aaggagatta aatgaaagtc agtattcttg attatggggt gattgataaa gaaaaaacac
    12001 cccaagaagc tttattagaa acgcgctgct tagcacaagt agccgacaaa cttggttttc
    12061 atcgtttttg ggtagcagag catcataata tttatgcctt tgcaataagc agtccagagc
    12121 tgttgatgat gcatttagca gatcatacca aacagattcg gataggttca ggaggtatta
    12181 tgcctctaca ttatagtagc tttaagattg cagaatggat aatgacttta gaggccttgc
    12241 accctaatcg gattgattta ggtattggca actctttagg aactacgtta gttcaaagag
    12301 ctctatcaag tatccattgc aaagacagct atagtcaggt tgtcacagaa ttataccagt
    12361 atcttaatcc tgatcatttg tcaccactac cgatttttgt taaccctagg ggtaatactt
    12421 accctcagat ttggacgcta agcaatagtt tggagacagc agaactggct ggtcaattag
    12481 gtttaggcta tacctttggt attttcccct atataccaaa agatcctata acagaagcta
    12541 aaagagtatc ggcacattat agaaaggctt ttaggccttc aaagctatta aaaatcccca
    12601 agttaatatt agcagttttt attgtccttt ctgataccga cgaggaagca gaagctttgg
    12661 ctaaaccgtt ggacatttgg atgcttggtc agcaagattt taatgaattt aagacctacc
    12721 ctgatgttga ggaggctaga aactatcact taacagagaa acaaagagaa gccattgcag
    12781 caaatcgtag tcgcatggtt attggatcac ctcacacagt caagaaacaa ttggataggc
    12841 tgatagaagc ttgtcaagct gacgaattat tagccatccc cttagttcct gaatttgcca
    12901 atagacaacg aaccttagag ctgctagcag acttatacgt gaccatttaa ccttaccaaa
    12961 aacaattccc taaactgaaa ggaaagaaac aatgaaaaaa attgcaaatt acttattaat
    13021 tgagaaaaca gatgaccgct ataccattag catgacacca gaattacaag acgatattgg
    13081 tactattggt tacgctgaat ttacggataa cgaccacctt gccgttgatg acattatctt
    13141 aaatctagag gcttctaaga ccgtcatgtc tgtcttgtca ccattggctg gggcagttgt
    13201 tgagcgcaat gaggcagcca cactaacacc aacccttttg aattcagaaa aagctgagga
    13261 aaattggatt gttgtgctaa cagatgttga tcaagcagct tttgatgcct tagaagatgc
    13321 ctagttcatt tgatttatta ggggaaatga ttggtctgtt gcaaacagag cagcttacta
    13381 gttcttgggc ctgtcccttg cctaatgctt taacaaagcg acaagacttg tggcgggcgc
    13441 tcattaacca gcgccctgct ttgcccttat caaaagatta cttgaatctg gaagatgctt
    13501 atctagatga ttggcgtgct tcttttgttc ctgtatcagt caaggattgc caaaaaacta
    13561 attataccag tctttttctt taccatgggg atattaggta ccttgctgtg gatgctattg
    13621 tcaatgctgc taatagtgag ttattgggat gttttattcc taaccatggc tgtattgata
    13681 atgcgattca tacgtttgcc ggaagtcgtt tacgcttagc ttgtcaggca atcatgactg
    13741 aacaaggccg taaggaagcc attggacaag caaagcttac cagtgcttat catctaccag
    13801 cctcttatat tattcatacc gtagggccaa gaattaccaa aggacaccac gtttctccga
    13861 ttcgagctga tttgttagca cgttgttatc gttctagtct tgatttggcg gttaaagcag
    13921 gattgactag cctagctttt tgctctattt caacaggaga atttggcttt cctaaaaaag
    13981 aagccgctca gattgctatt aagacagttt taaaatggca agcagaacat ccagaatcta
    14041 agaccttaac tatcattttt aataccttta catcagaaga caaggcctta tacgacactt
    14101 atttgcaaaa ggagaataac tgtgagtaat tggacaacct atcctcagaa aaacctcacc
    14161 caggcagaac aactggctca attaatcaaa gaagcagatg cgcttgtagt tggtattgga
    14221 gctggcatgt ctgcagctga tggttttact tatatcgggc cgcgttttga gacggcattc
    14281 ccagatttta ttgctaagta tcagtttttg gatatgctgc aagctagcct ttttgatttt
    14341 gaagattggc aagaatattg ggcttttcaa agtcgctttg tcgctttgaa ttatcttgat
    14401 cagcccgtag gccagtctta tcttgattta aaagagattt tggaaaccaa agactatcat
    14461 attattacta ctaatgctga taatgctttt tgggtggctg gttatgatcc tcataacatt
    14521 tttcacattc aaggagaata tggtttgtgg caatgtagcc agcactgcca tcagcaaact
    14581 tataaggacg atacggtcat ccgtcaaatg atcgcagagc aaaaaaacat gaaagtccca
    14641 ggtcaactga ttcctcattg ccctgaatgt gaggcgcctt ttgaaatcaa taagcgtaat
    14701 gaagaaaaag gaatggttga agatgctgac tttcatgccc aaaaagcacg ttatgaggct
    14761 tttttatcag aacataaaga gggtaaagtg ctttatctag aaattggtgt gggacatacg
    14821 acgcctcagt ttatcaaaca ccccttttgg aaacgtgtct cagaaaaccc taatgcctta
    14881 ttcgttacct tgaatcacaa acattaccgc atccctctgt caataaggcg acaaagccta
    14941 gaactgacag aacacattgc acaattgatt agcgccacaa aaaccattta ccaaaaatcg
    15001 taaccaaagc caatagacgg ttaattaaga gatagagatg gagactaaca tgtaccttat
    15061 tgaaccgatt cgtaacggta aacgtatcac cgatggagct gttgctctag ccatgcaagt
    15121 ttatgttcaa gaaaatctat ttttagatga tgatatttta tttccctact attgtgatcc
    15181 caaagttgaa attggaaaat tccagaatgc agttgtagaa actaatcaag agtatttaaa
    15241 agagcatcat atccctgttg ttcgcagaga tactggcggt ggtgccgttt atgtagattc
    15301 tggtgctgtt aatatttgct acttgattaa tgataatggt atttttggtg attttaaaag
    15361 aacctatcag cccgctattg aagcccttca tcatttggga gcaacagagg ttgaaatgtc
    15421 tggacggaat gacttggtta ttgatgggaa aaaggtatct ggcgctgcga tgactattgc
    15481 taatggccgt gtttatgggg gatattccct tttgctagat gtggactttg aagccatgga
    15541 aaaagcatta aaacctaatc gcaaaaaaat tgaatcaaaa ggcatcaggt cagttcgtag
    15601 tcgcgttgga aacatcagag aacatttggc accacaatac caaggtatta cgattgaaga
    15661 gttcaaagat ttaatggttt gccaattgtt acagattgag acgattagcc aagctaagcg
    15721 ttatgattta acggaaaaag actggcaaca aattgatgct ttaacagagc gtaaatatca
    15781 caattgggaa tggaattatg gcaacgcgcc tcaataccgt taccatagag atggccgttt
    15841 taccggtgga acagtagata ttcatttaga tattaaaaaa ggctatattg cagcttgtcg
    15901 catttacgga gatttttttg gcaaggcaga cattgcagaa cttgaaggtc acttgatagg
    15961 aacacgaatg gaaaaagagg atgtcttagc taccctcaat gcaattgatt tagcccctta
    16021 tttaggtgcg attacagcag aagaattagg atatttgata ttttcttgat aaaaaagata
    16081 cctcagaccg gtaaagtgcg attaaaaacg aacaataatt gttttaattc aaataaatgt
    16141 atgtatgata tgaattgtga tataatgacg tcattacgta aaattgagga gtcatcatga
    16201 aatcagatat cgaaattgcc caaagtgtcg ccttacaacc aatcactgac attgttaaaa
    16261 aggttggaat agacggcgat gacattgagc tttatggcaa atataaggct aaattgtctt
    16321 ttgagaaaat gaaagctgtc gaagctaatg agcctggaaa attaatcttg gtaactgcta
    16381 ttaaccctac tccagcagga gaaggaaaat caaccatgtc tattggactg gcagatgcct
    16441 taaatcaaat gggaaaaaaa accatgcttg ccttacgtga accctccctt ggtcctgtta
    16501 tggggattaa aggcggggca gcaggtggtg gttatgccca agtattgcct atggaagaca
    16561 ttaatcttca ctttactggt gacatgcatg ccattacaac cgctaataat gcattatctg
    16621 ctttgattga caatcatttg cagcaaggaa acgatttagg aattgatcct cgccgcatta
    16681 tttggaagcg cgtgttggac cttaatgatc gcgctcttcg tcaggtaatt gttggcttgg
    16741 gtagtccagt gaatggtgtg ccacgcgaag atgggtttga tatcacagta gcttctgaaa
    16801 tcatggcaat tttatgtttg gcaactgact taaaagatct aaagaaacgt cttgctgata
    16861 tcgtcgtggc ttatacctat gatcgtaagc ctgtctatgt gcgtgacttg aaggttgaag
    16921 gagcattgac attaattcta aaagatgcta tcaagccaaa cttggtgcag actatttatg
    16981 gaacaccagc tcttattcat ggggggccat ttgctaatat tgctcatggt tgtaattctg
    17041 tccttgctac ctcaacagct ttgcgattag cagattacac tgttaccgaa gcaggattcg
    17101 gggccgacct aggagctgaa aaattcctaa atatcaaagt acctaatctt cctaaagcac
    17161 cagatgctat tgtcattgtt gcaaccttgc gcgcccttaa aatgcatgga ggtgtcgcca
    17221 aatcagattt agccgctgaa aattgtgagg ctgttcgctt aggatttgcc aacttaaaac
    17281 gtcacgtgga aaacatgcgc caattcaaag ttccagttgt ggtggctatc aatgaatttg
    17341 tagcagatac cgaggctgaa attgctacct taaaagcgct ttgtgaagag attaaggttc
    17401 cagtagaatt agccagtgtt tgggcaaatg gagctgaggg aggcttagca ttagctaaaa
    17461 cagtggttcg tgtgattgat caagaagctg ctgactacaa acgtctctat tcagatgagg
    17521 atactttgga agaaaaagtg atcaatatcg ttacccagat ttatggtggc aaagctgttc
    17581 aatttggccc caaggccaaa acacaattga agcagttcgc agaattcggt tgggataaat
    17641 tgcctgtttg tatggctaag acccaataca gtttttcaga taacccatcc ttgcttggag
    17701 cgccaaccga ttttgacatt accattcgtg aatttgttcc taaaacagga gcaggtttta
    17761 ttgttggttt aacaggtgat gtgatgacca tgcctggttt gcctaaagtt ccagctgcca
    17821 tggcaatgga cgtagcagaa aatggcacag ccctcggtct cttttaggaa aaattgctcg
    17881 aataattcga gcaagcgctc ctttcttccc gctttactgt gatataataa ggtaaatgag
    17941 attttggagg gatgattatc aaaaagaaag caaaagtaaa atatttgctc cataaaggga
    18001 aacatggatt tttaagagga atttttagta gaacaacaat cattgtatta ttaattattt
    18061 tgcaattagt tttcttattt caatcttatg cctggatgga gcagtaccgt gtttggataa
    18121 cgattcttga aagtgtcttt gctattacta ttgttttata tttagttaat agtgatatgg
    18181 atgctatttc aagaatgacg tggttaattt tgattatgat tgctccatta ctagggtcac
    18241 tgtttttaat ttatacaaaa ttggattggg gttacagagg gctaaaacag agaataaatc
    18301 atcttgtaga tctgtctgca ccttacctta gcgatgatga cgctatatta gaggtattaa
    18361 aggatagcac atcaaccaca tatcatttag tacagtactt agaaagaagt cgtggcaact
    18421 ttccgattta taataataca agggtaactt acttcccaac aggtgaaact ttttttgata
    18481 gtttaaaaga acagttattc ttagctaaaa agtacatttt tcttgaattt tttattattg
    18541 cagaaggcca aatgtgggga gaaatcctta gtattctaga aaaaaaagtc agtgaaggtg
    18601 tagaagttag ggtgttgttt gatggcatga acgaactatc aacgctatca tcagattacg
    18661 ccaagagatt agaacaaata gggattaaag ctaaatcatt tttaccgatt tcacccttta
    18721 tctctaccta ttacaattat cgagatcacc gaaaaattgt cgttattgat ggggaggtat
    18781 cattcactgg aggtattaat ctagcagatg agtacattaa tgaagtagag cgttttggcc
    18841 gctggaaaga tgctggttta atgcttgagg gtgaagcaac agacagcttt ttaattttgt
    18901 ttttacaaat gtggtctatc acagaaaaag aactgattat tgatccttat ctttcagatc
    18961 attctttaaa acttccttca gatggctatg ttattcccta cggtgattcc ccgcttgata
    19021 ctgataaaat aggtaaaaat gtttatatag acattttaaa tcatgctaaa gagtacgttt
    19081 atatcatgac accttacctt attttagata gcgagatgga acacgcttta cgatttgcat
    19141 cagaacgtgg agtagatatc cgaattatta tgccaggagt gccagataag ggggtaccat
    19201 atgccttagc taaaacctat tataaagctt tgatgacttc tggagtaaaa atttatgagt
    19261 atcaaccagg gttcgttcat tcaaaggtat ttatctctga taatacaaaa gcagttgtcg
    19321 gaacaattaa cttagactat cgtagccttt atcatcattt tgaatgcgcg acttatttat
    19381 atcgtgtgtc agttattgct gatattgtga atgactttaa tgaagctcaa aagcaatcac
    19441 tattgatgac atcagatcat ttgaagcaac gtccttggta tcaaaaattg ataggattat
    19501 tagtacgaac aattgcccct ctcttgtaat tttcgtaaaa tatgttataa tatctgtatg
    19561 cgatgagtcg attgtgactg caaagtcact tttgcgcagg ggaggtcatt aaggcgggaa
    19621 gccgactttg ataatctgtg tgaaccattt tatcacaatc ttatttatta agaatgcccc
    19681 ttggattgag atattctcaa tccttttttt aattttatta acttagttaa aattcagaat
    19741 gtatcagtga ttactgtgtt tcaaaataag aaagtagttt ttcgggtatg agtgctatga
    19801 cttgacaaat gctaataaca tttgcataat gtaagtagag atgaaaactt atgaaaagag
    19861 atgatcttat gcaaattatt aagcgtgatg gccacgttgc tgattttgat ccagataaaa
    19921 tttatcaagc aattattaaa gcagcaagga cggtctatgt tattgatgac acgtggcgac
    19981 aaaacttaac acatgtcact aaaaaagtag ttattgattt agaagaagca catgtagaac
    20041 gtcctactat caatatggtc caatcccttg tggagaatcg tttgatggat gctggatata
    20101 ttaccattgc agaacattac atttcttatc gtcttcagcg agatttagag cgcaatggct
    20161 atggtgataa gattgtggtt cacttgcgtt ttgaacaaat aaaataaaaa cgcttctaat
    20221 ggcattttag tgtctttgag gggataaggt ttaaatacct aacagataaa aatctgttag
    20281 gtattttttt gctactatta ggtgtattct atattacata gaacttaagc attcaagcct
    20341 tagtttaact ttctgtttat tttttgctac attttgggaa actcccctcc tttcttagtt
    20401 gaggtgaata aacttttatt tgataaatca tatccttagt cttaatgaaa cagtctaaga
    20461 taatactact tcgaagtgac agctagtagt ttccaatttt taagtagtct aaaattatgt
    20521 ttttttaatt tcaaaaccat gctttcgttt gttataaaaa ccgatcttcc atagttagag
    20581 tttttatcca ggcattgaag attggggcaa ctatccaatt agagatttta taat
//
LOCUS       DAUCWO010000030        19756 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUCWO010000030 DAUCWO010000000
VERSION     DAUCWO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.030"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            7..927
                     /locus_tag="VGY20_001015"
     CDS             7..927
                     /locus_tag="VGY20_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ4273879.1"
                     /translation="MSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIADMTK
                     AIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFTKLVK
                     NAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLIAKDP
                     KNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGVPSAY
                     IWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYSEIFT
                     DSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            991..1716
                     /locus_tag="VGY20_001016"
     CDS             991..1716
                     /locus_tag="VGY20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273880.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            1717..2571
                     /locus_tag="VGY20_001017"
     CDS             1717..2571
                     /locus_tag="VGY20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ4273881.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(2719..3525)
                     /locus_tag="VGY20_001018"
     CDS             complement(2719..3525)
                     /locus_tag="VGY20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ4273882.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            3742..6147
                     /locus_tag="VGY20_001019"
     CDS             3742..6147
                     /locus_tag="VGY20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ4273883.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQAIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(6217..6570)
                     /locus_tag="VGY20_001020"
     CDS             complement(6217..6570)
                     /locus_tag="VGY20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ4273884.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            6814..7239
                     /gene="rplK"
                     /locus_tag="VGY20_001021"
     CDS             6814..7239
                     /gene="rplK"
                     /locus_tag="VGY20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ4273885.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            7345..8034
                     /gene="rplA"
                     /locus_tag="VGY20_001022"
     CDS             7345..8034
                     /gene="rplA"
                     /locus_tag="VGY20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ4273886.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            8357..9085
                     /locus_tag="VGY20_001023"
     CDS             8357..9085
                     /locus_tag="VGY20_001023"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ4273887.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            9115..9672
                     /gene="frr"
                     /locus_tag="VGY20_001024"
     CDS             9115..9672
                     /gene="frr"
                     /locus_tag="VGY20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ4273888.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            9780..10637
                     /locus_tag="VGY20_001025"
     CDS             9780..10637
                     /locus_tag="VGY20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ4273889.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLEVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            10710..11219
                     /gene="msrA"
                     /locus_tag="VGY20_001026"
     CDS             10710..11219
                     /gene="msrA"
                     /locus_tag="VGY20_001026"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ4273890.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            11216..11431
                     /locus_tag="VGY20_001027"
     CDS             11216..11431
                     /locus_tag="VGY20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ4273891.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            11587..12768
                     /locus_tag="VGY20_001028"
     CDS             11587..12768
                     /locus_tag="VGY20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ4273892.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMAPSATPSDVPTTPFASAKPDSSVT
                     ASYELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            13084..14856
                     /locus_tag="VGY20_001029"
     CDS             13084..14856
                     /locus_tag="VGY20_001029"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ4273893.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            15015..16067
                     /locus_tag="VGY20_001030"
     CDS             15015..16067
                     /locus_tag="VGY20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ4273894.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            16113..16688
                     /locus_tag="VGY20_001031"
     CDS             16113..16688
                     /locus_tag="VGY20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ4273895.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            16847..17344
                     /gene="ybeY"
                     /locus_tag="VGY20_001032"
     CDS             16847..17344
                     /gene="ybeY"
                     /locus_tag="VGY20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ4273896.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            17325..17732
                     /locus_tag="VGY20_001033"
     CDS             17325..17732
                     /locus_tag="VGY20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ4273897.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            17852..18748
                     /gene="era"
                     /locus_tag="VGY20_001034"
     CDS             17852..18748
                     /gene="era"
                     /locus_tag="VGY20_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ4273898.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            18768..19244
                     /locus_tag="VGY20_001035"
     CDS             18768..19244
                     /locus_tag="VGY20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4273899.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(19550..19729)
                     /locus_tag="VGY20_001036"
     CDS             complement(19550..19729)
                     /locus_tag="VGY20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273900.1"
                     /translation="MCYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSLRSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT         6110 a         3493 c         4262 g         5891 t
ORIGIN      
        1 aagagaatga gcctcatttt gggtgctttt ttatctgttt ttcttttagt agcttgttct
       61 tcaactggta ctaaaactgc taagagtgat aaattaaaag tcgtggcaac caattcaatt
      121 attgccgaca tgacaaaagc tattgctggt gataaaatcg atctgcacag cattgtgcca
      181 atcggtcaag atcctcatga gtacgaacca ttaccagaag atgttgaaaa aacaagtaat
      241 gctgatgtga ttttctataa tggtatcaat ctagaagatg gtgggcaagc ttggttcacc
      301 aaactagtga aaaatgctca aaaaacgaaa aacaaagatt actttgccgt gtctgatggc
      361 attgatgtga tttacttgga aggtgcaagc gaaaaaggaa aagaagatcc acatgcttgg
      421 ttaaatctcg aaaacggaat catttattca aaaaacattg ccaaacaatt gattgcaaag
      481 gatcctaaaa acaaagaaac ttatgaaaag aacctaaaag cttatgtggc taaattggaa
      541 aaactagaca aagaagccaa atcaaaattt gatgctattg cagaaaataa aaaattgatt
      601 gtgactagtg aaggttgctt caagtacttt tcaaaagctt acggtgtccc atctgcttat
      661 atctgggaaa ttaacaccga agaagaagga acaccagatc aaatttcatc attgattgaa
      721 aaactaaaag tcatcaagcc atctgcgctt tttgtagagt caagtgtcga tagacgccct
      781 atggaaactg tttctaaaga tagtggtatt cctatttatt ctgagatctt tacagattca
      841 attgctaaaa aaggtaaacc tggcgatagt tattatgcta tgatgaaatg gaaccttgac
      901 aaaatttctg aaggtctagc aaaataagat aacaataaag cacagcgatt ttagtgttgt
      961 gctttgtttt atttatgtaa aggagacaaa atgattacaa ctaacaatct ttgtgtgacc
     1021 tacgatggca acagtaatgc tttagaagcg attaatgtga caattgaagg tccttctatc
     1081 gttggtatta tcggtcctaa tggagctggg aaatcaacct ttatgaaagc tattttaaat
     1141 cttattgatt atcaaggtca cgtaacggtt gatggcaaag atggtcgtaa gcttgggcat
     1201 acagtagctt acgtagaaca acgaagcatg attgactata attttcctat tactgttaaa
     1261 gaatgtgtgg cgctagggac ttatagtaaa ttaggccttt ttcgccgagt aggaaaaaaa
     1321 caatttgagc aggttgataa ggttctcaaa caagttggtt tagaagactt tggccatcga
     1381 ccgataaaat ccttatctgg tggtcagttt cagcggatgt tggttgcgcg ttgcttgatt
     1441 caagaatcag actatatttt cttagatgaa ccgtttgttg gcattgattc ggtcagcgaa
     1501 aaaatcattg tagatctttt gaaagagctc aaaatggctg gcaaaacgat tttaattgta
     1561 catcatgatt tgagcaaagt agagcattat tttgacaaac tcatgatttt aaataaacat
     1621 ttggtggctt atggcaacgt ttgtgaggta tttactgttg ataccttgtc aaaagcttat
     1681 ggcaatcatt tgattttagg aaaggagatg gtgtaaatgt ctatgaaatt ttttgagggc
     1741 ttgatgtcat accacttttt acaaaatgca ctgataacgg ctgtagtcat tggtatcgtc
     1801 tcaggtgctg tagggtgttt tattatcctt aggtcaatgt ctcttatggg tgatgccatc
     1861 tcacacgctg ttttaccagg ggttgctttg tcatttattt taggagtcaa tttttttatt
     1921 ggagcgatta tttttgggtt attagcttct gttattatta cttatatcaa ggaaaactct
     1981 gtcattaaag gagatacggc tattggtatc acctttagct cttttttggc actgggagtc
     2041 atcttgatag gggtagccaa tagttcgacg gacttatttc atattttgtt tgggaatatt
     2101 ttagctgtgc aagacagcga taagtggatt actattggtg tttcgatttt tgttttaggg
     2161 gttattagtc tctttttcaa agaactatta ttaacatcat ttgatcctat cttagccaaa
     2221 tcaatgggtg tgaaggtcaa tgcgtatcat tatttgttga tggtcctatt aactttagtg
     2281 gctgttacgg cgatgcaaag cgtgggtact atcttgattg ttgccttact gattacgcca
     2341 gcagcgacag cttatttata tgctaatagc ttaaaagtaa tgttagtgat gtcatcttta
     2401 ctaggcgctt tagcatcggt tttaggactt tatctgggct atacctttaa tgttgccgca
     2461 gggtcaagta tcgtactgac ttctgcgatg atgtttttga tcagtttctt tgtttcaccc
     2521 aagcaaggct acctcaaaag atggatgcaa aaaaaggaaa aaacacctta aacttttcac
     2581 tttaaacacc ttagtggttt gcaaatagtg gcaaatgaca tcaaagggtg ttttttatat
     2641 gtcatcaaaa gaccataaaa aaccttatca aaccaaggta tttagcctat tatctgataa
     2701 ggttttttta agcatgactt agtttttaaa gcgataatct ttgacaatgt caattgaggt
     2761 aatcgtaatg tcttgttttg gtttatcatt ttggttgata gaagtcgcgg caattttatc
     2821 gacaacatcc ataccatcaa tgacttgacc aaatacagta taaccgccat ctaagcttgg
     2881 attgccgcca tgctcatagg cagagatgat aggttttggg tagttggtac ttgataatcc
     2941 cttgctttga tttttcttgt tttggttgat ataaaattgg ctaccgttag tattagcgcc
     3001 agcatttgcc atggcaagag caccgcgaat atgatataaa aatggagaga tttcgttgac
     3061 aaagccattg ccagcatctt ttttaggatc cttgcccttc caaatcgatt caccacctgt
     3121 gccatctcct ttggggtcac ctgattgaat cataaagtcg ttgatcacac gatggaaggt
     3181 aaggttatca tagtagcctt ttttagcatg ggtgaggaag ttctcaacag ctaagggagc
     3241 atattttggg aataacttaa gggtgatgtc cccttgactt gtcctcatta cgaccttagc
     3301 ttcttctttg ccgacttctt tggttagttg tggaaattgg ctagcatcag ctttgagagc
     3361 tttttgaatg ctttcttcat aggctttaga agctgcttcg ctttcttctt tggcagtttt
     3421 ttcatcaatg tatttatcgc cttttatagc gcggtcaaca gattcacaag cactcaaact
     3481 aagtaaacta atggctacta aactgagaga taataatttt ttcatgtcta ctcctaagaa
     3541 tgattttctt tattttatca taaaatgcaa cagaaaatag tagtaaagag ttaaaaacgc
     3601 ctatttttgt gttttatgat ctaaccttgg ttaaaaggct gctacgactg ttattatagc
     3661 acaagatgct atagaaaaac ttaaaacacc ttggtttttt agagctattt cctaatagtt
     3721 aaaaaatggt ataatagtgt tatggttaaa agaaatcaaa gaaaaaaaag tgcgcctaag
     3781 aagcgtttaa caaaagcaga agtggaaaag caacgtgcca ttaagaggat gattttatct
     3841 gttttaatgg ctttgttgct tatttttgcg atgcttcgct tgggcgtctt tggcgtgaca
     3901 acttataata tgattcgttt tttggtgggt agtttagcct atccgtttat gtttgcctgg
     3961 ttaatttact tattttgttt taaatggtta cggcaaaaag atggaatgat tgcgggagtt
     4021 gttattgcct ttttgggatt gctagttgag tggcacgcct tcttatttgc gatgcctagg
     4081 atgcttgatc aagctatttt tctaggcact gctcggctaa ttacaagaga tttactagcc
     4141 ctgcgcgtga ctgaatttgt tggtggagga atgcttggtg cattacttta taaacccata
     4201 gcctttttat tttcgaatat cggctcttac tttattggtt ttttgtttat cttattagga
     4261 ttatttctaa tgacgccttg ggatatctat gatgtgagtc actttgtgaa agaggctgtt
     4321 gataaactag cagtggctta tcaggaaaat aaagaaaagc ggttcatcaa acgagaagag
     4381 caccgcttac aagctgaaaa agaggcttta gagaaacaag cacaagagga agaaaaacgt
     4441 ctagcagaat tgacggttga tcctgagacg ggagagattg ttgaggatag tcagtctcaa
     4501 gtcagctatg atttggctga ggatatgaca aaagagccag agattcttgc ttatgatagt
     4561 cacctcaaag acgatgaggc aagcttattt gaccaagaag acttggctta tgctcatgag
     4621 gaaattgggg cttatgactc tttatctgct ttagcatctt ctgaagatga gatggatatg
     4681 gatgaacctg ttgaagttga tttcacacca aaaacccatc ttctttataa acttccaact
     4741 attgatttat ttgcaccaga taagcccaaa aaccagtcca aagagaaaaa cttagtccgc
     4801 aaaaatatca aggtgcttga agataccttc caaagttttg ggattgatgt caaggtagaa
     4861 cgagcagaaa tcgggccttc cgtcaccaaa tatgaaatta aaccagctgt cggggtccga
     4921 gttaatcgta tttcaaatct tgctgatgat ttggcacttg ctctggctgc taaagatgtt
     4981 cgtattgaag caccaattcc tggaaaatcg ttaataggta ttgaagttcc taactcagaa
     5041 attgcaacgg tttcttttcg cgaactttgg gaacaatctg atgccaatcc tgaaaacctt
     5101 ttagaagtac cactaggtaa agctgttaat ggcaacgctc gcagttttaa cttagctaga
     5161 atgccgcatc ttttggtagc tggttcaact ggttcaggta aatctgtggc agttaatggc
     5221 attatttcaa gtattttgat gaaggcacgt ccagatcaag ttaagtttat gatgattgac
     5281 cccaaaatgg ttgaattatc tgtttataat gatattccac atttattaat ccctgttgta
     5341 accaatccgc gtaaagccag taaggcactc caaaaagtgg ttgatgagat ggaaaaccgc
     5401 tacgagttat ttagtaaaat tggtgtgcgt aatatagcag gttataatac aaaggttgaa
     5461 gagtttaatg cttcttctga gcaaaaacaa ataccgttgc ctttaatcgt tgtcattgta
     5521 gatgaattgg ctgacttgat gatggtggct agtaaagaag ttgaagatgc tattattcgt
     5581 ttgggacaaa aagcacgtgc tgcaggtatc catatgattc ttgcaactca acgtccctct
     5641 gtagatgtta tttctggttt gattaaagca aatgttccgt cgcgtatggc atttgctgtt
     5701 tcaagtggta ctgatagccg tacaatcctt gatgaaaatg gtgctgaaaa gctcttggga
     5761 cggggtgata tgctctttaa gccgattgat gaaaatcacc ctgttcgctt gcaagggtct
     5821 ttcatctcag atgatgatgt ggagcgtatt gtcaacttta tcaaagacca agctgaagcg
     5881 gattatgatg acgcctttga tcctggtgaa gttagcgaca atgatcctgg tttttctgga
     5941 aatggcggag ctgctgaagg tgatcctctt tttgaagaag caaaagcctt ggtcttagaa
     6001 acacaaaaag ctagtgcctc aatgattcaa aggcgtttgt cggttggatt taatcgtgcc
     6061 acgcgcttga tggatgaatt ggaagaagca ggtgttatcg gtccagcaga aggaaccaaa
     6121 cctcgtaagg tattgcagac aaactaaaag aacctgacgt ctcatgatga gatgttaggt
     6181 tctttttttg tgatgtcagg ccagttgatg agatgcctat gctaatgtaa aaataaagat
     6241 aactagaata aggtagaaaa taacggtgac taaaaagcca actcgccaaa atagcttcat
     6301 aaagcgatgg taagaaaacg actgagttcg gatgactagc agtagtgaaa tgacgatggc
     6361 tagcagcgac atgataataa ggtaataagg cagtaaatta tgggtgaaaa attttcctga
     6421 gacaagcaca atttctatcg caaaaagtaa aagtgcgaca tctggaaatt taaatccctt
     6481 tttattaagt ttaaataaag aggttacaat catggcaaaa agaggcgtta ctatcaagaa
     6541 agctaaggca gttaatttat agatcatcat aggtctattt tatactcaaa tcccttactt
     6601 gtaaactgat acttacctgt tgtgatagaa aatgatcttt gagagggaaa gcgcctactt
     6661 caatttaaac attgcaaaag gcatcagcta ttttaacaaa aagacttgat ttattttggc
     6721 aataagctat aatagtaagg tatgcaatat tgcatatctg tgggaggtaa aaatctcaat
     6781 taccgccaaa accacaacag gaggattttt aaaatggcta aaaaagtcga aaaacttgta
     6841 aaacttcaaa tccctgctgg taaagctaca ccagctccac cagttggacc agctcttggt
     6901 caagcaggta tcaacatcat gggcttcact aaagaattta acgctcgtac agctgatcaa
     6961 gctggtatga tcatcccagt tgttatctca gtttatgaag acaaatcatt tgatttcatc
     7021 actaaaacac caccagctgc tgttcttttg aaaaaagctg caggtgttga aaaaggatca
     7081 ggtacaccta acactactaa ggttgcgaca gttactcgtg cacaagtaca agaaattgct
     7141 gaaactaaga tgccagattt gaacgctgca aacattgaag ctgcaatgcg tatgatcgaa
     7201 ggtactgctc gttctatggg attcactgtt actgactagt cagtcaagaa cccctattac
     7261 ccgcacgact tcatcataag agatgagtga cgtgggagat tttaaatcaa atcgatatga
     7321 ccacattaca aggagaattt taaaatggct aaaaaaagca aacaaatgcg tgctgcactt
     7381 gaaaaagtag atagcacaaa agcgtacagt gtagaagaag ctgtagcatt agtaaaagaa
     7441 acgaacttcg caaaatttga tgcgtctgtt gaagttgctt acaacttgaa cattgacgtt
     7501 cgtaaagcag accaacaaat ccgtggcgca atggtattgc caaacggaac tggtaaaaca
     7561 caacgtgttc ttgtttttgc acgtggtgca aaagctgaag aagcaaaagc agctggtgca
     7621 gacttcgtag gtgaagacga ccttgttgca aaaatcaatg gtggatggct tgactttgac
     7681 gttgttatcg caacgccaga catgatggct atcgtaggtc gtcttggacg tgtccttgga
     7741 cctcgtaact tgatgccaaa ccctaaaact ggtacagtaa cgatggatgt tgctaaagcc
     7801 gttgaagagt ctaaaggtgg taaaatcact taccgtgctg acaaagcagg taatgttcaa
     7861 gctcttattg gtaaagtttc atttgatgct gacaaattgg ttgaaaactt caaagccttc
     7921 cacgatgtaa tggctaaagc taaacctgca acagctaaag gaacttacat ggcaaacgtc
     7981 tcaatcacat caacacaagg tgttggtatc aaggttgatc ctaactcact ttaagaaaaa
     8041 aattagggca tcgcctaatc atagcaaaaa gacaaatttc tgaagacaaa tcttcactag
     8101 aggtttgtct ttttttatgg tttaaaatgg aatgcaaaca aaaaagtgta ggcgataata
     8161 ccatgaaagg agctgattct cttaatgaga tgtggtatta acacatcatg agctcacaaa
     8221 aaatgataaa ggaagtatcg gtggtgttaa aaaagggatt ttaattgata tttattagcc
     8281 tctaaaatgt cctttcccta ttcataattg agggtttgtg gtaaaatagt aaggacaaac
     8341 taaggagaac atagtagtgg aacctaaata tcaacgtata ttaattaagt tatctggtga
     8401 agcattagca ggtgaaaaag gagttggcat tgacattcca actgttcagg ctattgcaaa
     8461 agaaattgcc gaagttcatg tgtcgggagt acagatcgct cttgttattg gtggaggcaa
     8521 tctctggcgt ggagagcctg cagcagacgc tggaatggac cgtgttcagg ctgattacac
     8581 tggcatgctt ggaacggtga tgaatgccct agtcatggct gatagtcttc aacattatgg
     8641 tgttgatacc cgtgtgcaaa cagctattcc tatgcaaaat gtagcagaac cttatattcg
     8701 cggacgtgcc ttgcgccatc tagaaaaaaa tcggattgtt gtttttggag ctggtattgg
     8761 ctcgccttat ttttcaacag acaccaccgc ggcactgcgt gctgctgaga ttgaagcaga
     8821 tgctatttta atggcaaaaa atggtgtgga tggggtctat aatgctgatc ctaaaaaaga
     8881 tgctaatgcg gttaaatttg atgaattaac acatggtgaa gtgatcaaac gtggtctcaa
     8941 aatcatggat gcaacggcat cgaccttatc gatggacaac gatattgatc tggtggtttt
     9001 caatatgaat gaagctggca atattcaacg tgttgtcttt ggagaacata ttggaactac
     9061 tgtatcaaat aaagtttgtg actaaaagtt agaaaagaaa gagagtaaaa aaacatggca
     9121 aatgcaatta ttgaaactgc aaaagaacgt tttgcacaat cccatcagtc tttatcacgt
     9181 gaatatgcca gcattcgtgc gggtcgtgcc aacgctagtc ttttagaccg tatccaagtt
     9241 gattattacg gggcaccgac gccattaaat caattggctt caatcactgt gccagaagcg
     9301 cgtgtcttat tgatctcgcc ttttgataaa tcttctatca aagatatcga gcgcgctctt
     9361 aacgcatcag atttaggcat tacacctgct aacgatggct ctgtcattcg tttggttatt
     9421 ccagctttga ccgaagaaac acgtaaagaa ttagccaaag aagtgaaaaa agttggtgaa
     9481 aatgctaaaa ttgcaatccg taatatccgc cgtgatgcta tggatgatgc taaaaagcaa
     9541 gaaaaagcaa aagaaatcac tgaagatgag ttaaaaacac ttgagaaaga tatccaaaaa
     9601 gcaaccgatg atgctatcaa agaaattgac cgcatgacgg ctgaaaaaga aaaagaattg
     9661 ctctcagtgt aacaggattt ctacgaaaaa tagacgacta tagacacaaa aagataggtg
     9721 accatttcag aagcgtttgg ttttgatttg tccctatttt ttaaagaaag aaaaaagata
     9781 tgaatgattt actagcaaca gtcattactg ggcttattaa agaagaaaac gctaatgact
     9841 attttatcca aaaagagggg tttaccttta ccttgtctaa agcagaagga gaacgccaga
     9901 ttggtgatat ggtcacaggc tttgcctata cagacataaa gcaaaaggca cgtctcacaa
     9961 ccaaagagat ccgttcaaca cggacaagtt atggctgggg ggaagtgaca gaggttcgtc
    10021 gtgacctagg cgtttttgtc gatacaggaa ttccaaataa agaaattgtg gtttctttgg
    10081 acgtgttgcc tgaaatgaaa gaactatggc ctaaaaaagg ggataaactt tacattcgct
    10141 tagaagtgga taaaaaagac cgtatttggg gacttccagc agagcctgag gtctttcaaa
    10201 agatggctag ccctgcatac aataacatgc aaaatcaaca ttggccagca attgtttacc
    10261 gcctaaaact aacaggaacg tttgtttacc tgcctgaaaa caacatgttg ggctttattc
    10321 acccaagtga acgctacgca gaaccgcgct taggtcaggt tttagatgcc cgtgtgattg
    10381 gctttagaga ggtcgatcgt accctaaact tgtcgttgaa accacgttct tttgagatgt
    10441 tggagaatga cgctcagatg attgttactt atctggaagc caatggtggt ttcatgacct
    10501 taaatgacaa gtcaagtcca gaagagatta aggcaacctt tggtatttca aaaggccagt
    10561 ttaaaaaagc cttgggtgga ttgatgaaag ctaaacgcat caaacaagac gccacaggaa
    10621 ctgaattaat aggataaaaa tgctttgcca ctggcaaagt tttttgttac aatgaagacc
    10681 attaactgaa gaaaaagggg atatagagaa tggaaagagc catttttgca ggaggttgtt
    10741 tttggtgtat ggtgcaacct tttgaggagc aagcaggtat tttgtcagtc agaagtggct
    10801 acacaggtgg gcatctccca aatcctagct atgaacaagt ttgtgccaaa acaacaggac
    10861 atacagaagc tgttgaaatt atttttgacc ctaagcaaat cgcttataaa gatttggtag
    10921 agctctattg ggcgcagacg gatccaacag acgcctttgg gcaatttgaa gatcgtgggg
    10981 acaactaccg tcctgtcatt tactacacaa cggaaagaca aaaagaaatt gcagagcaat
    11041 caaaagcaaa cttgcaagct tcaggacgtt ttgaccaacc cattgtcacc actattgaac
    11101 cagcagagcc attttatctc gcagaggatt atcaccaagg tttctataaa aagaatccca
    11161 agcggtatgc acaaagcagt gctattcgtc atcagttttt agaggagaat tggtcatgag
    11221 aaaatcattt tacagctggc tgatgacaca acgtaaccct aaatcaaatg aacctgtggc
    11281 gattttagca gatttggtgt ttgatgacac cacttttcca aagcatacca atgattttga
    11341 actcattagt cggtatctag aagatcaagc tagtttttcc tttaatttag gtcaatttga
    11401 tgaaatctgg gaagattatc tagcacatta atacgtggat aaaaaagagt catatcgact
    11461 cttttgaagg gttatcacgt tattattaca aaatgacatc actttctttc aaaaaaatga
    11521 caagctactt tttttcttaa aaatatgcta tgatatagct tagaaaaaaa taaaaaggaa
    11581 gcagagatga ttattactaa aaagagctta tttgtgacaa gtgtcgcttt gtcgttagca
    11641 cctttggcga cagcgcaggc acaagagtgg acaccacgat cggttacaga aatcaagtct
    11701 gaactcgtcc tagttgataa tgtttttact tatactgtaa aatacggtga cactttaagc
    11761 acaattgctg aagcaatggg gattgatgtg catgtcttag gagatattaa tcatattgct
    11821 aatattgact taatttttcc agacacgatc ctaacagcca actacaacca acacggtcag
    11881 gcaacgactt tgacggttca agcgcctgct tctagtccag ctagcgttag tcatgtacct
    11941 agcagtgagc cattacccca agcatctgcc acctctcaat cgactgttcc tatggcacca
    12001 tctgcgacac catctgatgt cccaacgaca ccattcgcat ctgcaaagcc agatagttct
    12061 gtgacagcgt catatgagct cacatcgtca acgaatgatg tttcgactga gttgtctagc
    12121 gaatcacaaa agcagccaga agtaccacaa gaagcagttc caactcctaa agcagctgaa
    12181 acgactgaag tcgaacctaa gacagacatc tcagaagatt caacttcagc taataggcct
    12241 gtacctaacg agagtgcttc agaagaagtt tcttctgcgg ccccagcaca agctccagca
    12301 gaaaaagaag aaacctctca gatgttaact gcgccagcag cacaaaaagc tgtagctgac
    12361 accacaagtg ttgcaacctc aaacggcctt tcttacgctc caaaccatgc ctacaatcca
    12421 atgaatgcag ggcttcaacc acaaacagca gccttcaaag aagaagtggc ttctgccttt
    12481 ggtattacgt catttagtgg ttaccgtcca ggtgatccag gagatcatgg taaaggtttg
    12541 gccattgatt ttatggtgcc cgaaaattct gctcttggtg atcaagttgc tcaatatgcc
    12601 attgaccata tggcagagcg tggtatttca tacgttattt ggaaacagcg attctatgcg
    12661 ccatttgcaa gtatttacgg accagcctac acatggaacc ccatgccaga tcgcggcagt
    12721 attacagaaa accattatga tcatgttcat gtctccttta atgcttaatt catgctatca
    12781 aaaaaaactc acgtttaggc gtgagttttt tgacaattct gacagagtaa gccctataag
    12841 aggctgagat acgcttttaa tacctgttgt ttttctcacc ctttagacag ttttaacgtt
    12901 atgtctaaaa gagatggttt ttattagcgc aaatatgggt ttaggggtaa aatataggta
    12961 attaacctga tatgatcatg ataaaggtag aagaatacct attatttcgt gacaactgag
    13021 caatctgccc ttacctttgg agtgatgata tcactttgct tgactattca aggaggtctt
    13081 gaaatgtatt atactagtgg taattacgaa gcttttgcga cacctcgaaa acctgaaggg
    13141 gtagatcaga aatcggctta tattgttggt actggtttag ctggtttagc agcagctgtt
    13201 ttccttattc gcgatgggca tatggctggg gaacgcattc atctgtttga ggaattgcct
    13261 ttagcaggtg gttctttaga tggtattgaa aagcctcatc ttggttttgt gacccgtggt
    13321 ggtcgtgaga tggaaaatca ttttgagtgt atgtgggaca tgtatcggtc tattccctca
    13381 ctggaaattc ctggtgcgtc ttatttggat gaattttatt ggttggataa ggatgatcct
    13441 aactcatcca actgtcgttt gattcacaag agaggaaatc gtgtggatga tgacggccag
    13501 tatacgctcg gtaaacagtc aaaagaatta atccatttaa tcatgaagac agaagaatct
    13561 ctaggagatc aaaccattga agagttcttc tcagaagatt tctttaagag taatttttgg
    13621 atctattggg caaccatgtt tgcttttgaa aaatggcatt ctgctgtaga aatgcggcgc
    13681 tatgcgatga ggtttatcca ccatattgat ggtttgccag atttcacctc tctcaagttc
    13741 aacaaatata accaatatga ttctatggtc aaaccgatta ttgcttacct agaatcacac
    13801 gacgttgaca tccaatttga cacaaaagtc actgatattc aggtggaaca aacagctggt
    13861 aaaaaggtag caaaaaccat ccatatgacg gtgtctgggg aggctaaggc gattgagcta
    13921 acacctgatg atttggtttt tgtgaccaat ggttctatta ctgaaagtag cacatacggt
    13981 agtcatcacg aagtggctaa gccaaccaaa gcgttaggtg gttcttggaa tttatgggaa
    14041 aatctagctg ctcaatcaga tgattttggt catcctaaag tgttttacca ggacttgcct
    14101 gctgaaagct ggtttgtgtc tgccacagca accataaaac acccagctat cgagccttat
    14161 attgaacgtt tgacccaccg tgatttgcac gatggcaaag tgaacactgg cggcatcatc
    14221 actattacag attctaactg gatgatgagc tttgccattc accgtcaacc tcattttaaa
    14281 gaacaaaaag ataatgaaac cactgtctgg atttacggtc tttattccaa tagtgagggc
    14341 aattacgtcc acaagaaaat tgaggagtgt acaggtcaag aaatcacaga agaatggttg
    14401 taccaccttg gggtacctgt tgataaaatc aaggacttag cgagtcagga ctatatcaat
    14461 acagttcctg tttacatgcc ttatattacg agttacttta tgccacgtgt caaaggagac
    14521 cgtccgaaag ttatcccaga tggttcagtc aacttggcct ttattggtaa ctttgcggaa
    14581 tctccatctc gagatacggt ctttacgact gagtattcta ttcgtactgc catggaagca
    14641 gtgtatagct tcttgaatgt ggaacgaggc attccagaag tctttaattc agcctatgat
    14701 attcgtgaat tgctcaaagc cttttattac cttaatgata aaaaggcaat caaggatatg
    14761 gatttgccaa ttcctgcact gattgagaaa atcggacata aaaaaatcaa ggataccttt
    14821 atcgaagaat tgctcaaaga tgctaatctt atgtaacatg ctttagagta gttaaagctg
    14881 ataagtttag gcttatcagc ttttttgatg atttatagaa aagtgataaa ggaaaagtct
    14941 gacgtgaacg tcacataaga atttaaaatt tgttataatg taagcaataa agatgaaaga
    15001 aagagggact ctatttgcaa gagtattcta ttgacattac cttaacccat cctgatgatg
    15061 ttttagctct ttttggtagc aacgagcgtc atttgaagct aatagaagct catttaggag
    15121 ttattgttca cgcaagaacc gagcgagtcc aagtgattgg tgatgatgaa gaagctgtgg
    15181 aattggctcg attgaccatc aaagccctac tggttctagt gggtcgtggc atggtggtca
    15241 acacatcaga tgtggtgaca gctttatcta tggcagaatc tcatcagatt gaccaattta
    15301 tggcgcttta tgaagaagaa atcattaaag ataactatgg taaggcaata cgagtcaaaa
    15361 cattaggtca aaaaacctat gttgatagtg tcaaacgtca tgatgtggtt tttggtgtag
    15421 gacctgcagg gacagggaaa accttcttag ctgttacgct tgcagtcact gctctgaaaa
    15481 gaggacaggt aaagcgtatt attttgacgc gtcctgcggt agaagctggt gaaagcctag
    15541 gttttttacc aggcgatttg aaagaaaagg tggatcctta cctcagaccg gtttacgatg
    15601 ccctttatca cattttaggc aaggaacaaa ccacacgcct gatggaacga gatgtgattg
    15661 agattgctcc gttagcttat atgcggggtc gtactctgga tgacgctttt gttatcttag
    15721 atgaggctca aaacacgacc atcatgcaaa tgaagatgtt tttgacccgt cttggtttta
    15781 attctaaaat gattgtcaat ggagatacga gtcagattga tctccctcga aatgtcaagt
    15841 ctggtttgat tgatgctact cagaaattac aaggcattaa acagattgat tttgtctatt
    15901 tctctgctaa agatgttgtc cgacatcctg ttgtggctga tattatcaag gcttatgaaa
    15961 cgtcctcaga ggagatgaag gacttactaa aacctaaggc tagtgacgtt gaagctgact
    16021 accagccagg cttgacaaaa taccctgtga tcggtcaaga gcactaagac ctttacgatg
    16081 atgtttaata tggccttttt aaggaggatt ctatggatga tttaaccaaa gctattatgg
    16141 cagatgaggc taatctctcc tataccgaaa gaggtatttt tcctttatat gatgctcctc
    16201 aaacagctag gattattatt gttggccaag ctcctggaat agtagctcaa gaaaccaaac
    16261 tttactggaa tgatcgtagt ggcatccgct tacgagactg gctaggtgtg gacaatgaca
    16321 ccttttacca ctcaggattg tttggcatta tccctatgga tttctattat cctggtaagg
    16381 gaaaatccgg tgacttacca cctagagaag gttttgcggc taagtggcac ccgccattaa
    16441 gagcattgat gccagaagtt gaattgacga ttttggttgg tcgctatgct caggactttt
    16501 accttggtaa caaggcttac aaaaccttaa ctgaaacagt ccgtcacttt gaggattact
    16561 tgccagacta ttttccactt gttcatcctt ctccaagaaa ccaattgtgg ttagctaaga
    16621 acccctggtt tgagcaggat ttattaccta tcttgcaaaa acgtgtggag gcaattctaa
    16681 ccaaataggc cctgatttac ctagcattcc catttttaag gggcttgtgg tataatgggt
    16741 aaataacaaa tgctgattga aaggataagg gaaattatct gtcagataat aatgggtgcc
    16801 caacatacca aatgaggtgg gcagcccttc cctaacaatt aaccttatgt atatcgagat
    16861 gattgacgaa acgggacaag tttcgcaaga gattatggag caaacacttg atttgctcaa
    16921 ttttgctgct caaaagacgg gcaaagaaga aaaagaaatg tctgtgacct ttgtgaccaa
    16981 tgaaagaagt catgaactca acttagaata ccgagatact gatcgtccaa cagatgtgat
    17041 ttctctagaa tataagccag aaacgcctat tttatttagt caagaggatt tggctgctga
    17101 tcctagtttg gcagagatga tggcagagtt tgatgcttat attggcgaat tgtttatttc
    17161 tattgacaag gcgcgtgagc agtcccaaga atatgggcac tcttttgagc gtgaaatggg
    17221 atttttagct gttcatggat tcttacatat taatgggtat gatcactaca cccttgaaga
    17281 agaaaaagag atgtttacct tacaggaaga gattttgact gcttatggcc ttacacgaca
    17341 ataataccac caaacggaaa tggaaaaaca gaaccattac ctcaagcttg gagtttgcct
    17401 tgacaggggt tttcacagct tttaaggaag aacgcaatct cagaagtcac cttttgtctg
    17461 cctgtttagc ttgtgttgct ggtttatttt ttagcatttc agccatcgaa tggttatttt
    17521 tacttctagc catttttttg gtcattacct tagaaattgt caattctgct attgaaaatg
    17581 tagttgattt ggctagtgac tatcactttt ctatgctagc taaaaatgct aaggatatgg
    17641 cagcaggtgc tgttttgatg atttctggct atgctgtctt gacaggactt attattttta
    17701 ttcctaaaat ttggaatatt ttcgttcatt aaaaagaaag gaatgggcta tcctatgtga
    17761 tgatggcata ggagaataag gaaaggacaa gacggttaag cttagtgaca agcttgatct
    17821 gaagccctac cgttaaagcc cgtaattatc tatgtttaaa tcaggctttg tagccattct
    17881 tggtcgtcca aatgttggca aatccacctt tttaaatcac gtcatggggc aaaaaattgc
    17941 catcatgagt gacaaggctc agaccacccg taataaaatt atgggcattt acaccacaga
    18001 aacagaacaa attgtcttta ttgatacgcc aggtattcat aaaccaaaga ccgcccttgg
    18061 tgattttatg gttgaatctg cttatagtac tttacgtgaa gttgagacgg ttcttttcat
    18121 ggttcctgcc gatgaaaagc gtggtaaggg tgacgacatg attatcgagc ggctaaaagc
    18181 agctaagatt cccgttattt tagtcatcaa caaaattgat aaggttcatc cagaccaact
    18241 tttggaacag attgatgact tccgtagcca aatggatttt aaagaagtgg tcccaatttc
    18301 tgccctagaa ggtaataatg tgccaaccct aatcaagtta ttaactgata atcttgaaga
    18361 aggtttccaa tattttccag aagatcagat tactgatcat ccagaacgtt tccttgtgtc
    18421 agaaatggtt cgtgagaagg tactgcattt gacgcaacaa gaggttccac attccgtagc
    18481 tgttgttgtc gaatccatga agcgagatga agagactgat aaagtgcata tccgtgcaac
    18541 cattatggtg gagcgcgata gccaaaaagg catcatcatt ggaaaacaag gagccatgct
    18601 caaaaaaatc ggaaaaatgg cgcgccgtga tattgaactc atgttaggtg acaaggtcta
    18661 cttagaaacg tgggtcaaag tcaagaaaaa ctggcgggat aaaaagcttg atttagctga
    18721 ttttggctat aatgagaaag aatactaaca tagtaaagcg agatcatatg ccacaagact
    18781 acatttctta tatccgctcc aaagttgggc atgataagat tattcttaac tttgctggtg
    18841 ggattttgac caatgatgac ggcaaggtgc tgatgcagtt gcgtggtgat aagaaaacct
    18901 gggctattcc tggtggcact atggaactgg gtgagagctc tttagagact tgcaaacgtg
    18961 agttcttaga ggaaaccgga attgaggttg aagctgttcg cttactgaat gtttacactc
    19021 attttgagga agtttatccc aacggtgatg ctgttcagac tattgtcttt atctatgaac
    19081 taacggctgt ttctgatatg gctattgata actttcataa tgaggaaacg ctcaagttac
    19141 agtttttctc tcatgaggag atagcagagt tagagagtgt ctcagccaaa catcgcctga
    19201 tgttagagga atattttagt gatagctttg ctatggggca ttaagaactc tctaggacag
    19261 ttggagtatt tttggagatt ggcttgttaa aaaaattttt gaaagacgat gtttcgagag
    19321 cttatgctct ctttttttgc ttccaatttt tctgattaag tctgaataat tttgttaaat
    19381 gttaacagga ttttctttcc aattgatact agcagacatc aaacatttgt tttagagtta
    19441 tgacgaattg ccacctagcc atgcatgact taggcaagtt gaacctaact gtggcaatag
    19501 acttggaagg ggtgacaaga atcatttcct aaaagaattg atgaagtgcc tatccattat
    19561 tcgaattgta aagttcgtct aagtaaagtt gaatttggtc tcgaatggac cttaatgatt
    19621 tatgacttgt tgtgtcaata acaccaaaaa ctcgataatg atttttaaat atttcctcgt
    19681 aattaatatg atttccgatt agaataatat gagcatgagg ataacacata cgtatttcgt
    19741 ttagaaggtc atatac
//
LOCUS       DAUCWO010000031        18798 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUCWO010000031 DAUCWO010000000
VERSION     DAUCWO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.031"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            19..2019
                     /locus_tag="VGY20_001037"
     CDS             19..2019
                     /locus_tag="VGY20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ4273901.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(2043..2321)
                     /locus_tag="VGY20_001038"
     CDS             complement(2043..2321)
                     /locus_tag="VGY20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ4273902.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(2311..3165)
                     /locus_tag="VGY20_001039"
     CDS             complement(2311..3165)
                     /locus_tag="VGY20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ4273903.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGVAS
                     KGKPNHDH"
     gene            3205..3945
                     /locus_tag="VGY20_001040"
     CDS             3205..3945
                     /locus_tag="VGY20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ4273904.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKMAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            4144..4806
                     /locus_tag="VGY20_001041"
     CDS             4144..4806
                     /locus_tag="VGY20_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ4273905.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            4787..7030
                     /locus_tag="VGY20_001042"
     CDS             4787..7030
                     /locus_tag="VGY20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ4273906.1"
                     /translation="MTFSWTKLMPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGRTS
                     WHLETVR"
     gene            7101..8141
                     /locus_tag="VGY20_001043"
     CDS             7101..8141
                     /locus_tag="VGY20_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ4273907.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEASPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLRGKIDAARDLIHDLRLSGEDDIKLIAIMLGQFRLFLQLTILARDVKNEQQL
                     VISLSDILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            8238..8843
                     /locus_tag="VGY20_001044"
     CDS             8238..8843
                     /locus_tag="VGY20_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ4273908.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            9010..9233
                     /locus_tag="VGY20_001045"
                     /pseudo
     CDS             9010..9233
                     /locus_tag="VGY20_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9221..9448
                     /locus_tag="VGY20_001046"
     CDS             9221..9448
                     /locus_tag="VGY20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273909.1"
                     /translation="MDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSYKDLNSGR
                     TAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            9445..10022
                     /locus_tag="VGY20_001047"
                     /pseudo
     CDS             9445..10022
                     /locus_tag="VGY20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S11"
     gene            complement(10136..11356)
                     /locus_tag="VGY20_001048"
     CDS             complement(10136..11356)
                     /locus_tag="VGY20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ4273910.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQMEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(11363..12391)
                     /gene="queA"
                     /locus_tag="VGY20_001049"
     CDS             complement(11363..12391)
                     /gene="queA"
                     /locus_tag="VGY20_001049"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ4273911.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            12593..13297
                     /gene="nagB"
                     /locus_tag="VGY20_001050"
     CDS             12593..13297
                     /gene="nagB"
                     /locus_tag="VGY20_001050"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ4273912.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            13416..14132
                     /locus_tag="VGY20_001051"
     CDS             13416..14132
                     /locus_tag="VGY20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4273913.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            14429..15391
                     /locus_tag="VGY20_001052"
     CDS             14429..15391
                     /locus_tag="VGY20_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ4273914.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            15404..17209
                     /gene="pepF"
                     /locus_tag="VGY20_001053"
     CDS             15404..17209
                     /gene="pepF"
                     /locus_tag="VGY20_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ4273915.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFA
                     GHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNYFIQYEWA
                     RIPHFYYNYYVYQYATGFVAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            17584..18780
                     /locus_tag="VGY20_001054"
     CDS             17584..18780
                     /locus_tag="VGY20_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ4273916.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTSAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTSMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
BASE COUNT         5607 a         3329 c         3870 g         5992 t
ORIGIN      
        1 aattaggagg agtataagat gtctgatagc catcttactg cctttgacaa agcttcaaaa
       61 gcaggtttta ttattgcttt aggtattgtt tatggagata ttggaacaag ccccctttat
      121 acgatgcagt ctctagttga aaatcaagga ggggttaatc aggtttctga atcctttatt
      181 ttaggatcaa tctctttaat tatttggaca ctgaccttaa tcacaacaat caagtacgtt
      241 ttgattgctc taaaagcgga taaccaccat gaaggtggga ttttttcttt attcaccttg
      301 gtacgtaaaa tgtcaccgtg gctcattatt ccggcgatga ttggaggtgc aacacttttg
      361 tctgatgggg ctcttacacc tgcggtaaca gttacttcag ctattgaggg cttgaaagcc
      421 gttcctggtc tgagtcacat ctaccaaaat caaaccaatg tcattatcac aactctagtg
      481 attttaatag tcttgtttgg tattcagcgg tttggaacag gttttattgg taaaattttt
      541 ggaccagtta tgtttatctg gtttagtttt ctaggtgtta gtggtttttt taatacgttg
      601 gggcatctgg aaattttcaa ggctatcaat ccttactatg ctctgcattt gctgtttagt
      661 cctgaaaacc atcgtgggat ttttatcttg gggtccattt ttcttgcgac aacaggagct
      721 gaagcccttt attcggattt aggacatgtt ggtcgaggaa atatttatgt gagctggccc
      781 tttgttaaga tgtgtattgt gttgtcttac tgtggccagg ctgcgtggat tttagcgaat
      841 aaacactcag gtattgaact aaaccctttc tttgctagtg tgccatcaca attaagggtt
      901 taccttgtta gtttggcaac cttggcagct attattgcct cacaggctct tatttcaggc
      961 tcctttacct tggtttcaga ggccatgcgt ttgaaaattt tccctttgtt tcgtgtgact
     1021 tacccagggg caaatttggg acaattgtat attcctgtca ttaactggat tttatttgca
     1081 gtgacctctt gtactgtttt ggcttttaga acatcagccc acatggaggc agcttatggt
     1141 cttgctatta ccatcaccat gttgatgaca acaatcctct taaagtatta cttaatcaag
     1201 aaaggaacga gacccatctt ggctcattta gcgatggctt tctttgccct tgtagaattc
     1261 attttcttct tggcttcagc tatcaaattt atgcacggtg gctatgcggt tgttatcttg
     1321 gcacttgcaa ttgtctttgt catgtttatc tggcatgcgg gcacacggat tgttttcaag
     1381 tatgtgaaat ctcttaattt gaatgattac aaggagcaga ttaaacaact tcgtgacgac
     1441 gtttgttttg acctctacca aacaaatgtt gtctacctat ccaatcgtat gcaggatcat
     1501 atgattgacc ggtctatttt atactccatt ttagacaagc ggccaaaacg cgctcaagtt
     1561 tattggtttg taaatgtcca ggtaactgat gaaccttata cagctaagta caaggtagat
     1621 atgatgggaa cggattatat ggttcgagtc aacctttatc ttggttttag aatgccacag
     1681 actgtgccac gctatttgcg cacgattgtt caggatttga tggaaagtgg ccgtttaccc
     1741 aaacaagaac aggaatatac catcacacct ggtcgtgatg ttggtgattt tcgctttgtt
     1801 ttaatagaag aacgggtctc caatgcacgt caattgagta actttgaacg ttttatcatg
     1861 cagactaagg caagcattaa acatgttacc gctagtccaa tgcgttggtt tgggcttcaa
     1921 tattccgaag taacccttga agtggttccc ttgattctat cagatgtctt gaagttacca
     1981 atcaaagaat tggtgcctgt ggaagacagt gaagcataaa acaatcattg agatgataag
     2041 tctcaatgat ttttcttttc tttttgatga gtagcaatat aggccaattt ctggcttctt
     2101 gtttttcgtt ttttaaagag tgcttcagcg ctcatcgcag cctctttgct atcaaacact
     2161 tcgtaataaa gtaaagaaac aggtaaccgg tagcgagtat atttagcccc ctttcctgcg
     2221 ttatgtgtag ctaaacgctt tttcaaatca gttgtataac cagtgtagag ggttttatca
     2281 acacattcta acacatacat ataagctttt ttagtggtca tgattaggtt ttcctttgga
     2341 agcaacacca aaataaatct caaaaatatg atcagtgtat tctccgtttt ccttgtggac
     2401 aactaaagga ggtaaaatag tcatcccttc aatagaaccg tctttgattg cttcaataag
     2461 taacatatta gctgatttgc ctaatttagg atagacaaat tgtacccgtt taggggcaag
     2521 accattagct ctaaggctat ctataatttc caagaatcga tctggtcgat gaaccattgc
     2581 cagacgacca tttgatttaa gggcatgacg ggctacctga caaatctctt ctaaattagt
     2641 ggtcacttca tgacgagcca gtaaataatg ttcagagacg ttcttcttag aactttcgtg
     2701 acttttaaag taagggggat tgcagagcat caaatcaact cctgaacgtg gcacatggtt
     2761 taggaggttt tttaaatcat cacaaatcat ggttacttgg tcttccagtt gattgagctg
     2821 gatagagcgt tgtcccatat cagcaagccg ttcttgtaat tcgacttcaa cgatggctgc
     2881 cttggttcta gtgctagcaa ataagccaac cgccccattt cctgagcaca aatccacaat
     2941 taaccctttg gaaggcatct tggggaagcg ggataagagg acgctatcaa tagagtaact
     3001 aaagacatcc ttgttttgaa taatgccaac atcacttgaa aagagttgat caatacgttc
     3061 gccttctttt aagattgctt ttatcatacc tgttatttta acataaaata ggcttgaggt
     3121 gaatgtttga agagtgattt tgactttcga aaagtcagct ttcatgatac aataagtaag
     3181 attattctat taaaggagaa gcctgtgttt tacgcttact tacgcggttt agtagtcttt
     3241 ttattatggg ttgtcaatgg caatgcccac tatcatcatg aagaaaagat gttagatgct
     3301 tctgagaatt atattctggt agcaccccat cgaacctttt gggatccagt ttacatggca
     3361 tttgcagcac gtcctaagca gtttattttt atggctaaaa aagagttgtt tgcaaatcgt
     3421 ctctttgctt ggtggattaa aatgtgtggt gcttttccta ttgatcgtga caaaccgagt
     3481 ccagatgcta ttcgttaccc tgttaatatg ctcaaaaaga gcaatcgttc tcttcttatg
     3541 tttccaagtg gaagtaggca ttcgcaagag gtcaaaggag gggtcgctgt gattgctaaa
     3601 atggctaagg tcaagattat gccagcagcc tatcaggggc ctatgtcggt taagggatta
     3661 ctagcgggag aacgggttga catgactttt gggaatccca ttgacgtctc agatattaaa
     3721 cgcatgaatg atgaagggat tgctgaggtg gcaaaccgta ttcaggcaga gtttgatcgg
     3781 atggatgacg agttggcacc tttccaacca ggcaaagcaa ggaacccttt aacttatctc
     3841 taccgtttac cattaggatt ggttttggtg gtggtattat tattaaccat gttattttcc
     3901 tatattgcta gttttgtttg gaatccagat aaacatcgcc attaagaaca tgagggctaa
     3961 catgtgcctg aaataagaag tgattgagat tagtcttcta ttgaaagaag ccgctaatgc
     4021 ttaatcactt ttttcatttc taaaaaagat attcgatgac acccttaact cttttaagca
     4081 caaaaatgac aatactgcta tacttttgga tatcatttac gaataaaaag atgaggtaaa
     4141 aaaatgttag acgaattatt aattaaagga aaatcgtttt tgagagagcg gtattttctg
     4201 ccatacctaa ttagtctttt attaggactt tttctaatac taagcttcag ttttttgttc
     4261 tggggaaata ggcagagcaa ggcagccgtt cctgctttga gagaaatatc gcctgtgaaa
     4321 cagcaggtta gtgaagaaaa aaaggaaatt caagaagata gcagtatttt agtggattta
     4381 aaaggcgccg tacaaaaaga gggagtttat aagctaactg ctagcagtcg tgtgcgagat
     4441 gtcattgaac tagctggagg cctaacttca gaagctgata aacatgctat taattttgca
     4501 gaaaaactaa ctgatgagca agttgtttat gtacctaaac aaggagaaga gatttctgtg
     4561 ctaccaagat cactagtttc tggtaaaaaa gaaactgcca gcaaggatca gtcaaaggtt
     4621 catattaata aggccagctt agaagaacta caacatatcc caggtattgg tgctaaaaga
     4681 gctcaggata ttattgatat gagagataag ttaggtggtt ttaaagcgtt agaagatctc
     4741 cgtcaggttt cggggattgg cgaaaaaacg ctcgagaaat taaaagatga cattttcttg
     4801 gactaagcta atgcccctgt caaagattca atttgctttt ttaatcttgg ttctctttta
     4861 tcaaatccat agccctagct ggttaacgtt tctccttagc cttagtttga tttgcttact
     4921 tgtcaagcgt ttgtctaaaa aagaatttct cggtgtcttt gccatcctta gtttctgtgc
     4981 tttgtttttg ctgtatcaga aacagcagtt ggttcaaaaa ctggaaattc agccagtaca
     5041 aataacctct gtagcattag ttcctgatag tattcggatt aatggtgatc aattagctgt
     5101 tcttggacgt cacgggaaac attcttatca gttattttac cgtttgaaaa gccaagcaga
     5161 agcccaattg ttcaagaaag agcaccgttg gttagtgatg cacgcaaaag tgacattgga
     5221 aaaagctgag gaagttcgaa attttaaagg ctttaattat cagacttttt tggcttatca
     5281 aggaatttac aggataggga aggtagagca aatagaacag cttgaagtga tttcacccga
     5341 gtcaatatgt gactatttgt cctctttaag acggcgagcc attgttcatt gccagcagca
     5401 ttttccaaga cctatgtctc actacctgac gggcctcctt tttggttatt tagataagtc
     5461 gtttggtgag atgacagatt attatagtca gttgggtatc attcatctct ttgctttatc
     5521 agggatgcag gttggcttct ttcttacttg ttttagaagg gtgttgctat tactcgcagt
     5581 tcctttggaa tggataaaat ggatagagct tccttttgct tgcttttatg ccgcgttgac
     5641 aggttatagt atttctgtta ttcgaagcct cgtgcagtcg cagttgcgtc atttaggaat
     5701 aaaaggatta gacaatctgg cttgtacttt tttacttgtc tttttatggg atgctcattt
     5761 cttaatgact gttggtggcg tattaacgtt tagctatgct tttttgttaa cagtggttac
     5821 tgttgaagaa ttatcaggag ctaaaagaca gttggtacaa gtcttgacca tttccctagg
     5881 aatattacca tttttgctct tttatttttc cagttttaac ccaatgtcaa tggtcttaac
     5941 agctctcttg tcttatctct ttgacctttt tattttgccc cttctttgtc tggttttttg
     6001 tctatcccct ttggtgactg tctcaatatg caaccatctt tttattttat tagaaaaagt
     6061 cattcaattt ctcggaaata ctttcaatag ttcccttgtt tttggaagcc cgaccagttg
     6121 gcacttgctt atcttggtga ttagcttcgc tattttttat gattatcggc aggtgcgaca
     6181 acgagttatc acttgcggtt tggttatagc gttgacgctt ttgtctgtca aatatccttt
     6241 gaccaatgaa gtgaccttta tcgacattgg ccagggagat agtattttgg tgagggagtg
     6301 gacagggaaa aatctcttaa tagatgtagg tgggcgccct ttcttttctt caaaagagca
     6361 ctggcgacga ggacatcacg tagctaatgc tcagaaaacc ttaattcctt acttaaaaag
     6421 tagaggtatt cacacaattg accaactatt agtaactcac gcagatactg atcatatggg
     6481 ggatatcgaa gtggttgcca aagctattcg gataaaggag attttgacta gtcaaggcag
     6541 tttaagccat ccaagctttg ttaggcggct aaggcgttta aaatgtcacg ttagggtttt
     6601 agcagctgga gaccagttgc ctattatggg aagtgtctta caggtactct atccttggca
     6661 gttaggggac gggaaaaata acgattcttt ggtactttat ggcaggctct taaatcgaac
     6721 ttttcttttc acaggtgact tggaaaaaga gggggaaaac gagatcataa aacgttatcc
     6781 tcaactaaga gtagattatt taaaagcagg tcaccatgga tcaaatacct cttctagtgc
     6841 agctttttta gaccatattc agcctaaagt ggcctttatt tctgctggaa aaaataaccg
     6901 ttatcagcat cctcacagag aaaccttagc ccgtttggaa gacagacaaa tcacttatta
     6961 ccgaacggat acgcaaggag ctattcgttt gactggtcgg acaagctggc atcttgaaac
     7021 ggtccgttag ttaaaattcc agcctgacga agaaacaggc agtcgctttc cttttaggag
     7081 aaagatgata taataaaagc atgattgcga tagaaaagat tgaaaaactg agtaaagaaa
     7141 atttgggtct tataaccctt gtcacaggag atgacattgg tcagtatagc cagttgaaat
     7201 cccgcttaat ggagcagatt gcttttgata aggatgattt ggcctattct tactttgata
     7261 tgtctgaggc cgcttatcag gatgcagaaa tggatctagt gagcctaccc ttctttgctg
     7321 agcagaaggt ggtcattttt gaccatttgt tagatatcac gaccaataaa aaaagtttct
     7381 taaaagaaaa agacctaaag gcctttgaag cctatttaga aaatccctta gagactactc
     7441 gactaattat ctttgctcca ggtaaattgg atagtaagag acggcttgtt aagcttttga
     7501 aacgtgatgc ccttgtttta gaagccagcc ctctgaaaga agcagagcta agaacttatt
     7561 ttcaaaaata cagtcatcaa ctgggtttgg gatttgagag tggcgctttt gaacaactgc
     7621 ttttaaaatc aaacgatgat tttagtcaaa tcatgaaaaa catggccttt ttaaaagcct
     7681 ataaaaaaac gggaaatatt agcctaactg atattgagca agccattcct aaaagtttac
     7741 aagacaatat ttttgactta accagactcg ttctacgagg aaaaatcgat gcagctagag
     7801 atttgattca tgatttacgc ttatcagggg aagatgatat taaattaatt gctattatgt
     7861 tgggtcaatt tcgcttattt ttgcagctga ctattcttgc tagagacgta aaaaacgagc
     7921 aacaactagt gattagttta tcagatattc ttgggcggcg ggttaatcct taccaggtca
     7981 agtatgcgtt aaaggattct aggaccttat ctcttgcctt tctaacagga gcggtgaaaa
     8041 ccttgattga gacagattac cagataaaaa caggacttta tgagaagagt tatctagttg
     8101 atattgctct cttaaaaatc atgactcact ctcaaaaata ggcattggac cgctttccaa
     8161 gagataatca aattagttga aagctcttat tttttgcgat aagatagacg tatcataaag
     8221 gaaaagagga caggaaaatg gctattattt taccagaact tccatacgcg tatgatgctc
     8281 ttgaaccaca gtttgatgcg gagacaatga ctcttcatca tgataaacac catgccactt
     8341 atgttgctaa taccaatgcg gctttagaaa aacacccaga gatcggagaa aatctggagg
     8401 aactattggc agatgtcact aagattccag aagatattcg tcaagcctta attaataatg
     8461 gtggcggaca cctaaaccat gcgcttttct gggaactatt atcacctgaa aaacaagacg
     8521 tcactcctga tgttgcacaa gccattgatg atgcttttgg gtcatttgac gcctttaaag
     8581 aacaatttac agcagctgct actggacgtt ttggttcagg ttgggcatgg ttagttgtta
     8641 ataaagaagg acaactcgaa atcacatcaa ccgctaacca agatactcca atttcagaag
     8701 gcaaaaaacc aattctagcg cttgatgtgt gggagcatgc ttactacttg aattatcgta
     8761 atgtacgtcc aaattatatc aaagctttct ttgaaatcat taattggaaa aaagtatcag
     8821 aactttacca agccgctaaa taagaaaagg gtttccaatc tcaaaaagag attggttttt
     8881 taagtgcttt tgtgaataac agtgtgggga catcattttt tagaaaagat aaaaatagtt
     8941 tgtcttttta atgtaattcc ttgattttta gctaaattta gctagaatag aatggatagg
     9001 agattttagg tgttaagaaa gaagaaaact accattgtag ggattatttt aggaatgatg
     9061 atagtgacct tacttgtcgg ctgtttgact ggtaaaaaga aaggctttcc tcctaaagca
     9121 ggtgagcggc agcaaacagc tgacttaaaa atgaaaaaag attagaaaaa ctgttcaaaa
     9181 accgttacct gaaaaatcta aagaggctaa gcaagctgtt atggatgcct tagggctcta
     9241 ttacgatgac aaccatttga gtttggtcaa tacggtcaag gtgtatttaa aggaatttgg
     9301 cattggtgaa tctcaaattg ccttttccta caaagacctc aattcaggca ggactgctgc
     9361 catgaatgat catcagccaa tgattgcagg aagcacctat aagctgccat taaacatgct
     9421 ggtggtagat aaggcaaatt atccatgaca gagcgttttg acattacaga aactgattat
     9481 gaatatgatc aggaacatca tgcctatgtt gctcaattca atggtgccat gtctattccg
     9541 gatatgcaag atactcagaa atcacgccag cttatgctct agcagaacga ttgggtggca
     9601 tgaataaggc ttaccaatta tttgaccgct acgggaaggt atccggggct attacgacca
     9661 ttgatcgtaa tggcaataaa atcaccaccg cttactattt gcaagttttg gattacttgt
     9721 ggcagcatca ggataaatat aaggatatct tgtactacat tggtgaatct ttccctgatt
     9781 tgtattacaa gacttatttg tctcatgtaa aggtttatca aaaaccgggt tatgtcagag
     9841 aagccttaaa tgtaggtgcc attgtttgcg aagagtctcc ttatttgatt gctctttatt
     9901 catctggttt gggtggagca acacaagcat cagaggaagt caatggcctt ggttatgtgc
     9961 agcttgttca gctaacatat gtaattaatg agtggcatag agtcaatcac aacatgatct
    10021 aatactcagt tgacattagt ctgatagtag acatacttac tgatgacagt cactaaaata
    10081 agttgttgct cattaaaaaa gcttggcctg tttcttcagg tcaagctttt tgagattaat
    10141 gctgtgtttt tgaggtctta ttgtcacctc ttgtttcttg ataatggtga agcaattttt
    10201 tgaatttttt aacggcagcg cgtgttagct gcttagatga tttgccattt ctgctgtaaa
    10261 tgggattgtg cttatccctt tgctgttcta aatcgggaac tagaaaagtt gcatcaggac
    10321 tattaattgc tttttcaata aaagccggat caaaaagcag aatagggtca atatcaagca
    10381 gacggtgcaa gtcatcgttt tggttgccat aattgaaaga agaaccattg acaatactat
    10441 ttcgcgttag gttatcagga tctagtgcta ctaaaaattg tccagattct gacaaaataa
    10501 actctgagtg aaaatgaatc aaaatcttat agttggcttg aggaatattg tctgtataga
    10561 ctgcctgatg attgccatta gcatctaaaa tactagcaac cttattatgg tatctagaag
    10621 attcataaag agcataatcg tcatcttcta gagtagccaa atatttggct aaggcatccg
    10681 catcggtttt ccctttattc ccataatgtg agcgaaccca ctccatttgc tgggttgata
    10741 tgaggtaacg gagttgatga attttccgat taaaacggct ataaggaatg tgttcgagat
    10801 taacataata agcctgaatc agggtagata attggtccca aaaaggatct gttggcgata
    10861 gctttggact taattgtgcc aatacttgct gtgtttgttt tgattgtcct gtcaactcgt
    10921 ctggcatctt taaatacgtc aagagcagtt gtaaccgttt ttgagcttct tgaggcgtct
    10981 gtgccatctc ttcttgttgc caaagctttt gaaagtcagg tgatcctatc aggcgaatgc
    11041 gactataaag ttgttcgaaa tgataagagt ttcctctaac agcgagttgt tcaaggtatc
    11101 ctttttggat ggctttatag gaccatccgt ccttatcgag ctttcgaaca taatcaagca
    11161 agtcgtggcg taatttaata gcttctgaaa gctggagttc tcgtaattta ggatagtttt
    11221 ggtaaaaagg tgccactaaa tcttgataaa gtttgtgttc tttttcaaag gcaatccgct
    11281 gatagtcttt ctgccaaaaa ctgacgcaaa aagtcagaaa catcaagaag aatagccatt
    11341 ttccaagtaa tttcataggt ttttagttga caaacatggc atcgccaaag ctaaagaaac
    11401 gatacttttc gtcaaccgcg tgtctgtaag cttcaaggac aaagtcacgc cctgcaaagg
    11461 ctgatactag catcacaagg gttgattttg gaagatggaa attagttgaa aaagcatcaa
    11521 ctaccttgaa ttggtagcct ggtttaataa aaatattggt ccaacctgaa tcagcttgaa
    11581 tgtccccttg gaacttactc ccgatggttt ccaacgtacg aatagaggtg gttcctacag
    11641 ctacaacacg accgccagct tgtttgacgt ctcttagggt ttgcgctgct tcttctgaga
    11701 gggaataaaa ttccgaatgc atatcatgct catcgaggtt atccacggaa acaggtctaa
    11761 aagtcccaag ccctacatgt agggtcaaat aaaccaaatg tacccctttg gcttcgattt
    11821 ttttgagcaa gtctgttgtg aagtggagac cagcagttgg ggcggccgca gagccatttt
    11881 ctttagcata aacggtttgg tatcgttcag catcctctaa tttttcgtga atataaggag
    11941 gcaatggcat ttcgccgagg ctctctaaaa cttctaagaa aatcccatca tagctgaatt
    12001 caacaattcg tccaccatgt tctaattcgt cgataatggt ggctttgaga cgcccatctc
    12061 caaaattgac ttggctacca accttgagcc gtttggctgg cttggctaaa acttcccatt
    12121 gatctccttg tgtattttta agcaaaagca attccacatg accatgagta tcgggttttt
    12181 ctccataaag tctggcaggt aagacacggg tattattcat gacgagggca tcccctggat
    12241 ttaattgatc aatgatatgg tcaaaatggc tatcaaccat agttttttga cggtgatcaa
    12301 taatcaatag ttttgaactg tctctttttt cgagtggggt ttgggcaatc aattcttctg
    12361 gtaactcaaa atcaaaatcg ttagtattca ttgggactcc tttaaagcca ctagtaaatc
    12421 tcttcctagt aactctttca tgtaagataa atcttttcta ttatatcact tgtcgtctaa
    12481 cccgtcatct ttaatctttc atcagaaagt aaaggatagg cttgacaaaa attggtatat
    12541 accatataat aaaaacacga aaggtctata ccaattagaa aaggagaatg acatgaaaat
    12601 tattcgggtc caagatcaga ttgaaggtgg gaaaatcgct tttaccttat tgaaagatag
    12661 cttagctaag ggagctaaga cgttaggatt agcaacagga agttcaccaa ttagttttta
    12721 ccaagaaatg gtcaaaagtc ctttggattt ttcggactta acaagtatta acttggatga
    12781 atatgtcggt ctttcagtag agagcgatca gagttatgat tattttatgc gtcaaaacct
    12841 ttttaatgcg aagcctttta aaaagaatta cttgcccaat ggtttagcaa ctgatatcga
    12901 agctgaagct aaacgatatg accaaatcat tgcagagcat ccgattgatt ttcaagtgct
    12961 tgggattggg cgtaatgggc acattggttt taacgaacct gggacctctt ttgaagaaga
    13021 aacgcatgtg gtggatcttc aagaatcaac cattgaagct aatagtcgtt tctttacaag
    13081 tattgaggat gtgccaaaac aagctatctc tatgggaatt gcctccatca tgaagtccaa
    13141 aatgattgtt cttcttgcct ttggacaaga aaaagctgat gcgatcaagg gaatggtttt
    13201 tggaccaatc acagaagacc tcccagcaag tattctccaa aaacatgatc atgttatcgt
    13261 cattgtggat gaagctgctg cgtcacaatt agactaacct aaccagtcgt taaaccttgt
    13321 aagaacaaaa atgaggagat agtcaacctc atttaagaag aagtcttgta acttctattt
    13381 cttttgcaag ggtaaattta tgttacacta ctaatatgcg tttagataaa ttattagaag
    13441 gtacaaaagt tggctctaga agtcaagtga agaaactgat taaggctcaa ggggtttggg
    13501 ttgatcacat gcctgccaga aatggtcgac aaaatgttga tcctggcttg caactgattg
    13561 aggtgactgg acagcgagtg acgcatccta aacattctta cattatcctc aacaagccat
    13621 caggggtggt ctcagctaag aaagatacta actatttaac cgttattgat cagcttgctg
    13681 aagaagataa aagtccagac ttgtatcctg ttggcagatt ggatagagat accgaagggt
    13741 tggttttact cactgataat ggtcccctag ggtttcggat gttgcaccct agtcatcatg
    13801 tttctaaaac gtatcttgta accgttaatg gcttgttagc agaagatgcc tcagattttt
    13861 ttgcagctgg tatttgtttc ccaacaggtg aacaatgcca accagctcaa ttgactattc
    13921 ttaaagccga tacagaccag tcacaggcta gtttgaccat ttcagaagga aaatttcacc
    13981 aagtgaaaaa aatgtttttg acctatggtt tgaaagtgac ctctctgaaa cggacgcact
    14041 ttgcaggact ggaactcggt catttagcca gcggagaata tcgttatcta acagcgtcag
    14101 aaaggcaact cattaaaacg tatctggact gagcaaaaca tgctttcaaa aagaagctat
    14161 tcttgcttaa tcaataaagt gtccattgac tattatagtc atgatgagtc cgacttttgc
    14221 tatgtttagt catcttgttg ttacaaaatc agataagcaa aaagattcaa ttcccactat
    14281 ctccttaacc tgaagcgtta taaaaaccct tgtgttaaac tctcataaaa ttggaaatgg
    14341 gagtgttttt tgatggctaa aaaaaactaa agctacttaa agtttggtac ttagttttcg
    14401 aataataagg tggaagatct aataggaggt gacaaaaata ttaaccgctt tagatggtaa
    14461 gaatcagctc atttcgctag taactcagcc tatttcaacg aaaccgcctt ttcgttgccc
    14521 agcttgcaaa tctcctgttc gcttgcgtca ggggacaatt agacgacctc attttgccca
    14581 tgtacaacta gctcattgtc aattccaagc agaaaatgaa tctgaggaac atttgacgtt
    14641 gaaggcaaaa ctttacacca gtttagtccg aacagaagct gtctgtattg aaaaatatct
    14701 tcctgagctg caacagatag cagacctttg ggtgaatgat aagttagcct tagaaattca
    14761 gtgcagtccc ttgccagttg agcgtctaaa gaaacgcacc aaagcctatc aagaaaaggg
    14821 gtatccggta cgatggttac tgggaagaaa attgtggctt aacactcact taactgcttt
    14881 gcaaaaacag tttctttact tttcatctag tttgggcttt catttgtggg agctggatgc
    14941 agctgcaaat ctgctacgtc tcaaatattt aatccatgaa gacttatttg gtaaggtaag
    15001 ttacctaacc aaaaccattt ctttagacca caatattatg gaaatgtttc gattacccta
    15061 tcagcaagaa atcctttatt cttatcagaa aaaaatgact gtgaacctat ctaaaagaat
    15121 acaaagagct cttttagcta gacacccgaa atggctacga cgacaagaaa aggcttactt
    15181 atcaggctat aatttactta tgttaacaac tgatgctttt tatcctcaat ggagaccagt
    15241 tcaatcttcc agcggttttt gtcaaattaa aggaaatctt cgcccttatt atgaaagttt
    15301 taaagtctat tataaaaaag aaaaggataa gaaggtgcaa acgctttttt caccaaaata
    15361 ttatgttaaa atggatagta atagaaaatg agaaggagat gttatgactg ataatcgcag
    15421 ccatttagaa gaaaaataca cttgggattt gagcactatt tttgcaacag ataaggattg
    15481 ggaagctgaa gtcagtgact tagctactga agttgaggca tctaaaggtt ttgctggtca
    15541 tttgctggat tctagtgcta atttgttaaa ggttacaaaa acatatttag aattggctcg
    15601 tcgtgttgag aaagtctatg tctatgctca tatgaaaaat gatcaagaca cgactgttgc
    15661 taaatatcaa gaatatcagg ctaaggcttc tggcttgtat gctaaattta gcgaagtgtt
    15721 ttcattctat gacccagaag ttatggcatt gagtcaggaa gattaccaag cttttcttgc
    15781 ggaaacacct gatttaaaaa ggtatcatca tttctttgac aaattgttcc agacacgtga
    15841 ccatgtgctc agccaggctg aagaggaatt attagcagga gcccaagaaa ttttcaacgg
    15901 cgctgaagaa acctttagta ttcttgataa cgctgatatt gtctttccag tagttaaaaa
    15961 tgataagggg gaagatgttg aattaaccca tggtaacttc attagcctaa tggaatctaa
    16021 ggaccgtagc attcgccaag ctgcttatga agccatgtat agtacttatg aacagttcca
    16081 acatacttac gctaaaacct tgcaaaccaa tgtgaaagtg caaaactata aggcgcgtgt
    16141 tcataaatac gattcagcac gtcaagctgc catggctgct aactttattc cagaagctgt
    16201 ttacgacact ttattagaaa cggttaacaa acatttacca ctcttacacc gttatttgaa
    16261 attgcgtcaa gaggttcttg gtttagacga tttgaagatg tatgatgttt acacaccatt
    16321 atctgaaaca gatttagcca ttggttatga tgaggctttg gaaaaggctg agaaggtttt
    16381 ggctgttttt ggtaaagatt acgcagatcg tgtgcatcgt gcttttaccg aacgttggat
    16441 tgatgttcat gtcaataaag ggaagcgttc tggagcttat tcgggtggtt cctatgatac
    16501 caatgccttt atgctattaa actggcaaga taccttagac aatctataca ctttagttca
    16561 tgaaactggg cacagtttgc attctacttt cactcgtgaa acacagcctt atgtgtatgg
    16621 agattacagt attttcttgg cagaaattgc atccacaacg aatgaaaata ttatgacaga
    16681 agctctctta aatgaggttc aagatgagaa agaacgcttt gccattttaa accattacct
    16741 cgatggtttc cgtggaacag ttttccgtca aacccaattt gctgagtttg aacatgccat
    16801 tcatcaagct gatcaaaaag gagaagtgtt aacaagtgaa taccttaacc agctatacgc
    16861 tgatttaaat gaaaaatatt atggtttaag taaaaaagat aattatttta ttcagtatga
    16921 atgggcacgt attcctcatt tctattacaa ttattatgtt taccaatatg caacaggttt
    16981 tgtagctgca agctatttag cggataagat tgtgcatggc acacaagacg atatcgatca
    17041 ttacttggcc tacctcaagt caggaaattc tgattaccca cttgaagtga ttgctaaagc
    17101 aggcgttgat atggcaaaag gtgattattt ggaagccgcc tttaaagtct ttgacgaacg
    17161 tctaacagaa cttgaagtac tcgtgtccaa aggcatacac aaaagctaaa atcacaaaag
    17221 tcaaggacaa aaagagttta ggattgatag aaatggagat tcacagttgg tgtgaatctc
    17281 catttctatc ggtcattttc tttttatatt ttagtggatt atcagtcctg aaaagcaggc
    17341 ttcattgacc ttagtttgac ggtttctttt ttgtcttttt tattgtgtgg tcactcactt
    17401 aaataaaacc aatagttgag taaggcgctt cagattaacg ctattctatt gttttagaga
    17461 taatgttcgc ataaaacata tttttattat atttgacatt ttataaaaga gataatagaa
    17521 ttaaaatact aacatttatg tttttttaaa aataaacgtt cgtataataa aaaggagttt
    17581 ttcatggaaa aaacaaaacg ttatatcatt gcaacagcag ggatacttct ccacttaatg
    17641 ttgggatcga cctatgcttg gagtgtttat cgtaatccaa ttttacaaga gaccggttgg
    17701 gaccaagctc ctgtcgcttt tgccttttct ttagctattt tttgtttagg tttatctgct
    17761 gcttttatgg gaaatttagt tgaacaatat ggtcctcgtc taaccggaac ggtgtcagct
    17821 attctttatg cttctggaaa tatgctgact ggtttagcga ttgatcgtaa agaaatttgg
    17881 cttttatata ttggttatgg tgttattggt ggcttaggac ttggagcggg atatatcaca
    17941 ccgatatcaa ctattataaa atggtttcca gataaaagag gcatggcaac agggtttgct
    18001 attatggggt ttggttttgc gtctttatta acaagtccga ttgcccagtg gctaattgaa
    18061 acagaaggtt tggtagcaac attttacctt ttagggctga tttatctaat agttatgctt
    18121 tttgcttctc aattgataat aaaaccaact tctgctgaga tagctatact agataaaaaa
    18181 agattacaaa ataacagtta tttaatcgaa ggaatgaccg ctaaagaagc acttaaaaca
    18241 aaatcatttt attgtctttg ggtaatttta tttatcaata tcacatgtgg cttaggactt
    18301 atctcagttg ttgcgccaat ggcacaagat ctaactagta tgagtccaga aatgtcagca
    18361 attgtggttg gtgctatggg gatttttaac ggtttcggtc gtttagtgtg ggctagtttg
    18421 tcagactata ttggacgccg tgtgacagta atacttttat ttttagtaag cataattatg
    18481 actattagtt taatatttgc acatagttcc ctaattttca tgataagcat tgccaccctt
    18541 atgacttgtt atggtgcggg tttttctctc attccgcctt atttaagtga tctttttggg
    18601 gcaaaggaac tggctacatt acatgggtat atattgacag cttgggcaat agctgcatta
    18661 actggaccta tgttattatc tatcactgtt gaatggactc ataattatct tcttacctta
    18721 tgtgttttta ttgtcttata cattttagga ttaatggtag cattaaggtt aaagaaatga
    18781 tgtcctataa aaaattta
//
LOCUS       DAUCWO010000032        18399 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUCWO010000032 DAUCWO010000000
VERSION     DAUCWO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18399
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.032"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            316..393
                     /locus_tag="VGY20_001055"
                     /pseudo
     CDS             316..393
                     /locus_tag="VGY20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(491..2368)
                     /locus_tag="VGY20_001056"
     CDS             complement(491..2368)
                     /locus_tag="VGY20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ4273917.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDAASVLDTVLSADVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDAANLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDTGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLTNFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRVFYGNYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKRRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYDQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(2365..3573)
                     /locus_tag="VGY20_001057"
     CDS             complement(2365..3573)
                     /locus_tag="VGY20_001057"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ4273918.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLGRPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEATTFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDR
                     INTLDQALVIHDKHDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(3698..4546)
                     /locus_tag="VGY20_001058"
     CDS             complement(3698..4546)
                     /locus_tag="VGY20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4273919.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(4546..4926)
                     /locus_tag="VGY20_001059"
     CDS             complement(4546..4926)
                     /locus_tag="VGY20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ4273920.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(5173..5330)
                     /locus_tag="VGY20_001060"
                     /pseudo
     CDS             complement(5173..5330)
                     /locus_tag="VGY20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            5829..6854
                     /locus_tag="VGY20_001061"
     CDS             5829..6854
                     /locus_tag="VGY20_001061"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEQ4273921.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVT
                     PYHVTSVWTKGVTPTANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWLDQNNEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            6874..7239
                     /locus_tag="VGY20_001062"
     CDS             6874..7239
                     /locus_tag="VGY20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273922.1"
                     /translation="MSSTDDGILRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEF
                     DFKIRKFLMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKD
                     NKTINMKDFSHFDIYLWTK"
     gene            7443..7754
                     /locus_tag="VGY20_001063"
                     /pseudo
     CDS             7443..7754
                     /locus_tag="VGY20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7811..8518)
                     /locus_tag="VGY20_001064"
     CDS             complement(7811..8518)
                     /locus_tag="VGY20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4273923.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSAAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(8669..9268)
                     /locus_tag="VGY20_001065"
     CDS             complement(8669..9268)
                     /locus_tag="VGY20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4273924.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDSLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            complement(9361..11307)
                     /locus_tag="VGY20_001066"
     CDS             complement(9361..11307)
                     /locus_tag="VGY20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4273925.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMIREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            complement(11304..12215)
                     /gene="pfkB"
                     /locus_tag="VGY20_001067"
     CDS             complement(11304..12215)
                     /gene="pfkB"
                     /locus_tag="VGY20_001067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ4273926.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDITSTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISREQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            complement(12212..12925)
                     /locus_tag="VGY20_001068"
     CDS             complement(12212..12925)
                     /locus_tag="VGY20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4273927.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            complement(13181..14104)
                     /locus_tag="VGY20_001069"
     CDS             complement(13181..14104)
                     /locus_tag="VGY20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ4273928.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     IVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
                     ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVKLNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            complement(14117..15175)
                     /locus_tag="VGY20_001070"
     CDS             complement(14117..15175)
                     /locus_tag="VGY20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ4273929.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            complement(15353..16345)
                     /locus_tag="VGY20_001071"
     CDS             complement(15353..16345)
                     /locus_tag="VGY20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4273930.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGAELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            complement(16345..17076)
                     /gene="trmD"
                     /locus_tag="VGY20_001072"
     CDS             complement(16345..17076)
                     /gene="trmD"
                     /locus_tag="VGY20_001072"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ4273931.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            complement(17066..17584)
                     /gene="rimM"
                     /locus_tag="VGY20_001073"
     CDS             complement(17066..17584)
                     /gene="rimM"
                     /locus_tag="VGY20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ4273932.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            complement(17799..18320)
                     /locus_tag="VGY20_001074"
     CDS             complement(17799..18320)
                     /locus_tag="VGY20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4273933.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFVETLKWWLHQRQRMS
                     ANELLKYYLELIK"
BASE COUNT         5785 a         3851 c         3014 g         5749 t
ORIGIN      
        1 tgtcaagttt ttcagttgac ttaacttttt tacttgattt ttcctcacgt aataatagaa
       61 aagaagcgcc ataatgtaaa ctacacccca aacgttagat aaaaaaatct aacgtttggg
      121 gtgtttttgt atgacattaa gctatgaaga taagctagat atttagcgct aaaaaaaacc
      181 gagggaactc cgtttgaaag aagcatctct gcaaagaaga agacaaaaaa ccattataaa
      241 gaatggacaa agacgagctc tgattagatt ttcttttcag aacggctaaa gtgactcgtt
      301 ctacctacta ttatcatcga ttattgcaac aataaacgta tcaaaataaa acgaaaagga
      361 cttagtcctc tacaacacag aactaaatcc tttacttcaa atagagtagc caacttttgg
      421 ggctcagtgc aagagcactt cttttctatt atcacttgtt tattataaac ataacattac
      481 catagtatac ttatatgtct agtccactca aatactcaaa ccgttcatac gccaataata
      541 aatcattatt gcgctgatcg agttcctttt gtaattgagc taactggtca taatcactaa
      601 ctacagttaa catttggttt tcaatttctt caatattagc ctcaatagtt gctattttat
      661 cttctatctg agcccattct tgtttttcaa ggtaggacat gcgccttttc tcctctttcg
      721 gaggaagtgg tacttcttca gtgactattg ctttagctaa gtcagcttca actgtttctt
      781 tttcaaaagc tttttcatca aggtaatcac tgtaattgcc ataaaaaaca cgaatatcgc
      841 cttcttcaaa ggctaaaatc ttagtggcta ctttgtctag gaaataacga tcgtgactaa
      901 ccgtaatgac tggtcctgca aagttagtta aaaagttttc taagaccgtc aaggtggcaa
      961 tgtctaaatc atttgttggc tcatcaagga gaagaacatt tggcttttca atgagcagtt
     1021 ttagcaagta gagccgtttt ttctccccac cagataattt agcaatcagc gtgccatgac
     1081 ttgatctcgg aaaaagaaat tgttctaaaa gctcactgat actcgttgtt cccacgcttg
     1141 ttttaacctc atcagccact tcttgaaggt aattaatgac ccgtttggtc tcatccatat
     1201 ctttgagctg ctgagagaaa taacctacac gaatagtatc tccagtatca agcttcccag
     1261 aagttggttt gagatcaccg ttgatgatat ttaacagggt agattttccg acgccgttat
     1321 ctccaacaat tccaatacga tctttatttt gaataatcaa gttaaaatcc ttaatcaact
     1381 gtcggtcgcc ataagcaaaa gaaaggtctt caaagtgaat caccttcttc ccaattcggc
     1441 tcgtttcgaa agccatctcc aacttatccg cagaactgtc ttggtgaact tcttttttca
     1501 agtcagtgaa acgattgatt ctagcctgct gtttggttgc acgcgcctgt ggctgcgtcc
     1561 tcatccaggc taattcttgt ttatagagtt gcttcttctt gtgtaaatta gcagcatctc
     1621 gttcgtcttg ttctgctttt aagcgcacat agtcttggta attgccttga tattctgtca
     1681 gtcccgcttt atccaattca aaaatacggg tagccacatg atcaaggaaa taacggtcat
     1741 gggtaataaa aaggacggtt tttttagctg tcttgagata ggttgttaac caagcgatgg
     1801 tatcaatatc gagatggttg gtcggctcat ctaataggag taagtcagca gctcctagca
     1861 atacttgagc tagctggaca cgtcttctca taccacctga taggtctccc actttttgtt
     1921 ccaaatccgt gatgcctaat tttgatagga ctgttttgac atcactttca atagaccaag
     1981 catctaagcg atccatttca gacatgaggg actctaaggt ctcttgttta tcttcagtat
     2041 aattcgccat caatagttca tactgacgaa tcagctgaat cgccttaaca tcagctgaca
     2101 ataccgtatc taaaacagag gctgcatcgt taaattcagg atcttgtgtt aagtaagcga
     2161 ttttgtaatc attggcttta gaaaatggtg aatggtctcc gtcaaaacca agccttcccg
     2221 acaagacatc taaaagagtt gtttttcctg tcccattaac cccaataatc ccaatacggt
     2281 caaagtcatg aataataaag gaaatatctt gaaagacggt cttatctccc accgtttttg
     2341 tcaatttctc caccaaaaaa tggctcatgt tgctaattcc tctctaacaa atgcttcaat
     2401 tgtagtgaaa tcattatcta ggcgcccctc tacaatcgct aattcaactt tctcaagcat
     2461 aagccctagc tgaggacctg gtttcatccc aaaatcctta atcaggtgag aaccattaag
     2521 gacgatatcg tgtttgtcat gaataaccag tgcctgatct aaagtattaa tacgatccat
     2581 gtcaacggat aagctttgag cttttcggag gtcttccact agactcgcca ttttcttgcc
     2641 gtactggtaa atatccaatt tttcaaaaga tctttcctga cgaattctat aaagagcaat
     2701 caactttgtg acataacgct gaaaatcatt tgaagtcttc catgctttta ggaagctttt
     2761 aggatcctca atggctaaac taatcatcac ataagcccaa gcttggtgat aatcactaaa
     2821 ggtaaagtta tctttcaaag aaactatcag atgattcaag cctgcttcct gctgcttcaa
     2881 gcctggtaaa tagtcgtaag cctgacaagc aatcatggca gaaatccctt ttcgccagtg
     2941 cggtgccatc aataatttat caaattcagt aaaagaacgt tccacagaaa ttttttccaa
     3001 tagaggtgaa tggctcctca tagcttcaaa agttgtagct tcaatgtcaa aatccagact
     3061 cgccgcaaat cgaaagcctc tcataatgcg tagtgcatct tcttcaaaac gttcctctgc
     3121 tttacccact gctcgtaacc gtttttgctt taaatcaatg agtcctctaa atttatcaat
     3181 aacttgacca ttttcatcca aggccaaggc attgacggta aagtctcttc gttttaagtc
     3241 ttcctctaaa gagcgcacaa aagaaacctg actaggacgg cgataatcaa catagacatc
     3301 ttctgtccta aaggttgtga tttcatactc accgccattt tcaagcacga gcactgtacc
     3361 atgttcaatg ccaacatcga cagttctgtt aaaaatcgct ttagtctctt caggatagga
     3421 actagttgca atatctacat catgaatggg acgcccgagt aaaacatctc tgacgcttcc
     3481 tcccacaaag taggcttcat aaccagcttc ttttatttta gtcaatatgg gtaaagcctt
     3541 ctgaaactca gaaggcattg tcattaattt catacgttgt tttctaacct atcatccaac
     3601 tagcccaatc taaagtgtga ttaggctact tgggttctat cacattttgt tattgtttta
     3661 gtgattcttg caattaacta ttttttctaa tcgacaatta aagcctcggt gttacgccaa
     3721 atgcaatggc tccttctcct aaatgagtag caatcaccgc tccaaaatga acctcttcaa
     3781 tgtcatattg ataaccactg tcttgtaaga gtcgtttcaa ataatcagct ttgtcttgtg
     3841 cttttgagtg aataatagac acattatact gaccgtctgc tatcaagtca tttaaaatct
     3901 caaccaagcg tttcatggct ttcttttctg ttctcacttt ttcataaaca acgatttttc
     3961 cctcttcatc aaagcgtaaa ataggtttaa tactcaaaag atttccaaga agagcagacc
     4021 catttgacag gcgtccgcct ttaaccagat gattaaggtc atcaaccata ataaaggctg
     4081 tcgtcccatc aatctgttct tgcagcttgt ttaaaatagc ttgaaatgtc attccttgac
     4141 gtgaccaatc tagcacattt tttaccatac tacctaaagg agcagatgtg atcttactgt
     4201 ctggaaatgc catttcaatc tcaggatgtt cttcagctaa aaattgaata ttttgccaaa
     4261 agcctgagat tcctccagct aagaacaagc caataacatg tgtataaccc ttagaagaca
     4321 aaagccctaa aagattatct agttcagata aacttggctg acttgtcttg ggaagatttt
     4381 gggagtcagc catcttttga taaaaatcat ctatactcaa atttcgacct tcaaaatagg
     4441 tctcatcgtc aatgataact ggtatatcaa ggctaaaaat agctttatct tgtttcagat
     4501 ctgttggtag tgttgctgta ctgtcagtaa ttactgctaa tttcattaat ttttaaactc
     4561 caaatgaata cctggacgat ccaaggcaat ttctgtgact tcataagtca atcgcttaac
     4621 catgtctagc aaaggagcag ctaaggattc aacttcctct tgcgtaaatt cgcttggttt
     4681 atccactaaa cgatctaaaa tgcgaaccat ttgtgagatg accccactaa taacaaattc
     4741 ttctttgaca ataacaaatt gcaatacaga tacaattact gtttcttttt gctgctcatc
     4801 tttatcaatc aactgaaaag tcacttcaaa attagtctct ggtgttccgt tttctttttc
     4861 ccactctaaa tttctagcat cataatggta ctggttgaca aattcttttt cacgaactaa
     4921 ttgcattttt tattctcctt ggtatcaata tgatccatta tagcatagat ttgaaaatta
     4981 ttaaagtagc ccctaaacta ctattttcgc ataacgtttt aaacagtgct atctctcgaa
     5041 cagatatcta ttttttcacc tatttatatt tagtcgtcct tatactgaca ttatatcaaa
     5101 aaaacacccg ttagtggtct tgtactgcac ctcaattaaa acagaaccac tggtcaggta
     5161 agttttaagt caaggataaa aaggtttatc attaaggcct catcatgctt agccaagtat
     5221 ttcgaactag cttgtcttag ctctagtcgc tcaaacgtaa atcctaggcg ttcaatccgc
     5281 cgcaaaacct ctccaactaa cccatactta aacccatcgg gcttaatcat cgctccgccc
     5341 ttcttctttt atccattata aaataaaaac gttttaacag acaaaaaata ggaaagtacc
     5401 agactattgg atttattttt ttatctcaaa ctccattaac gatgttgagt taaaggcatt
     5461 caaaacaaca tagatcgtaa gctaacaaga ttttttatca attgaaccta tcgtccccag
     5521 ttataccacg acctcttctg gatgaggtct ttttttattt gtctaatttc atcaacgctt
     5581 cttcccttct ttcttctatc acagctctcc tattgctcac ttcctcatgc agcaacatat
     5641 catcaaaaaa ccgttcattt ttaagcacaa ccactactgt atctcttgtg atgtctccat
     5701 tgcaatcgct tgtttaattt tattaaaaat tatttaaaaa attaaatatt attttaagaa
     5761 cttgcaataa tatatatcta tattatataa tttctttatc ataaaattat atagaaaaag
     5821 gtattcttat gagaaaaaga tgctattcaa cttcagctgc agtattggca gcagtgactt
     5881 tatttgctct atcggtagat cgtggtgtta tagcagatag tttttctgct aatcaagaga
     5941 ttagatattc ggaagtaaca ccttatcacg ttacttccgt ttggaccaaa ggagttactc
     6001 ctacagcaaa cttcactcaa ggcgaagatg tttttcacgc tccttatgtt gctaaccaag
     6061 gatggtatga tattaccaaa acattcaatg gaaaagacga tcttctttgc ggggctgcca
     6121 cagcagggaa tatgcttcac tggtggttag atcaaaacaa cgaaaaaatt gaagcatatc
     6181 taaaaaaaca cccagataaa caaaaaatca tgtttggtga tcaagaatta ttggatgtaa
     6241 gaaaagttat taatactaaa ggtgaccaaa caaatagcga gctttttaat tatttccgag
     6301 ataaagcttt ccccggtttg tcagcacgcc gaattggagt tatgcctgat cttgttttag
     6361 atatgtttat caatggttat tacttaaatg tttataagac acagactact gatgtcaata
     6421 gaacctatca agagaaagat cgccgaggtg gtatttttga cgccgtattt acaagaggtg
     6481 atcaaagtaa gctattgaca agtcgtcatg attttaaaga aaaaaatctc aaagaaatca
     6541 gtgatctcat taagaaagag ttaaccgaag gcaaggctct aggcctatca cacacctacg
     6601 ctaacgtacg catcaaccat gttataaacc tgtggggagc tgacttcgat tctaacggga
     6661 accttaaagc tatttatgta acagactctg atagtaatgc atctattggt atgaagaaat
     6721 actttgttgg tgttaattcc gctggaaaag tagctatttc tgctaaagaa ataaaagaag
     6781 ataatattgg tgctcaagta ctagggttat ttacactttc aacagggcaa gatagttgga
     6841 atcagaccaa ttaaagcgta tcaataagaa agaatatctt caacagatga tggtattcta
     6901 aggactaata ctattgcagg gaattttttt atagatgggc aacagcaaaa aacattaata
     6961 aatcctataa aaatcgataa ggatattgtt acgattcaag agttcgattt taaaataaga
     7021 aaatttttga tggaatcaaa agaaattcat ttgactaagt ctccttatat aagggggagt
     7081 ttagaaattc acagtaaaaa taggaaacat gaaaaaatca atttatatga cgcaaaaccc
     7141 aatagcacca gatctgatgt cttaaaaaaa tataaagaca ataagactat aaacatgaaa
     7201 gatttcagcc attttgatat ttatctttgg actaaataag ctaaaagctt tggataaata
     7261 tgatcaattg acctataata acattgatac caaatctaaa actgctcaga ttgaattgcg
     7321 ccccaaaagt tagacaaaaa atctaataat tggggtgttt ttgtctggca ttaagtttag
     7381 ctaataacct tgcttaaatc acattaaacc aataatggaa aaagacatct ccaaataaat
     7441 gctatgatgg aactatatga tagagtgatc aaggttggca atatcaacac aactattatc
     7501 atcatttcct aaactctaaa ggaattcgtc cgtctatgtc acgtaaaaga aacagtcctg
     7561 acaatggcat gatggaatct ttctttggca tcttaaagtc agaaatgttc tatggttatg
     7621 agaataacta taaatcacta gaagacttag aacaagcgat tacaaatgac attgattatt
     7681 acaacgataa acatatcaaa gtaaaactaa aaggatttag ttctgtacaa tacagaacta
     7741 aatcctttac ttaaaataga gtatccagct tttttggggt cagtgcaagc agattgttgt
     7801 tagtgttaca ttaataagtc atgcgtttag cccaagcctc atcaactgct agggtctccg
     7861 catcagtgta attacgacga tactcattcc aaacataacc cgaagttgct agaccagttt
     7921 cttgagctgc gctattagca acatcataag ccgtcaaacc ataggttgca ataatattat
     7981 ttagttttaa gttataactg gtgtctgtag cgtataagcc agttagagct gctgtagcat
     8041 cttgatacga tagcgtgttt gattttctag cacctatata agtgctagaa cttagcaagt
     8101 ctgcatagtc attaagggag tctgcaatac ttggataagc tctaaaggct tgatcaatcg
     8161 tataggtatt gccattacca tcatcttccc aagttgacat cgtcacagaa gaaccgttat
     8221 aggcaccctt aataccgaaa aaattataat aaggtgcttg acttagactt gatttaccat
     8281 tacttgattc taaaatcgct tgtgctatca taacagacgc ataaagatca cgttcttgac
     8341 caatagtact agcagttgga ccaatttcat caataaaatg tcgggttgaa gaactggcga
     8401 tagtctcagc tgacacgttt gaagttttat gagattgtct cattgcacta taagctccta
     8461 aacaagctgc tagaacaaaa agactgataa gtacaagctt acctttcttt tttgtcatta
     8521 ttcacatatc cttttttata cattttctat gtttctatta tagcacacga acgtttaaat
     8581 taacttttcc acagataatt taatgagatt gataactttt gtcttgaaat cgacatattt
     8641 cttctgattt ttttaaaatt gtggataact atttcattag accatcaaaa ttagtgagtt
     8701 gataccattc aatcaattca tttaatttgg tatggtaagt tgtatcagtg gcgtaagtgc
     8761 ctgttaaggt tgcagtagca tcttgataat gagaccaaaa ctttttgtgg gttttgtcat
     8821 aaagtgaatc attcaaaact cttgcgtaat ctaaaaaaca agctgtcagg ctcccatagg
     8881 aacgaaaagc agcatcaatt tgataaagat tcccttgtcc atcgtcttct aaagttggaa
     8941 aaatgactga ccgttcttta tagctcccct taatcccgaa aaaattataa taaggttttt
     9001 gacttagttg tgacttgcca ttattggatt ctaaaatggc ctgagccatc atcaccgaac
     9061 tgtataactg tttcttttga gcgactgatt gagcagcatg actaatcttc ctgacaaatg
     9121 tcagagtttc gtgatccgca tacggagcta atcgctggtt tcccaatttt agtgtccaca
     9181 tactaacaat cagattaagt aatactaacc ctaagacaag tcgatttact gatgatttag
     9241 cccttcttct cctttttttc accttcaaag gtcctcgttt cttatccttt aactctagta
     9301 tagcaaaaaa ctagaacctt gacagattct agttttagta agaaaaaagt tctttacctt
     9361 ttaggctttt ttgcgaagag cgccaaataa gatacctgat acaagggcgc caatcacaac
     9421 aaagacaaga tataagattg gattgcttgt taaagcgatt acgaagatac caccatgtgg
     9481 cgccattaat ttaataccag ccaaaccaac aagagctcct gtcaaggcag aacctgcgat
     9541 aaagctagga atcgcacgtg ctgggtcagc tgcaccaaac ggaattgctc cttcggtgat
     9601 gaatgaaagt cccataacaa tatttgtcaa gcctgattca cgttcttctt tggtaaattt
     9661 atctttaaac aagagagtgg ctacaaaaac agcaagagga ggtaccatac cacctgccat
     9721 aacagcggcc ataacgaccg aaccaccatt tgccacagta gctgctaaag ttcctgtacc
     9781 aaaaacataa gctgccttat taacaggacc acccatgtca acagccatca ttccaccaac
     9841 aagaagtccc ataaggacag cagagcttcc tgaaagacct tgtaagaaat cattaagggc
     9901 tgtgttaata gctgccattg gaatattgac aaaaagcatc aagaaaccag tgacaaggac
     9961 gccaagtaat gggtaaagga ggatagattt aaccccttct agagaacgtg gtaggcctgc
    10021 caacaattta cgaagggcaa gaataacacc accggcaagg aaaccaccca caagcgcccc
    10081 aaggaaacct gatggcacgc ccgttaagcc caagctcact tctcctcctg ctgcaaatgg
    10141 taccttacca aaagcaagac catttgaggc aatggcacct gccacaaaac cagctactaa
    10201 gcccggtttt tcagcaatag aataagcgat ataacctgct agtactggta acatgaagga
    10261 aaaggctgct ccaccaatgt tcataaaaat agctgctatt tcatgatagg atccaagact
    10321 tccaagctga tcatttggca caccaagcat gttatccaat aaaaatgcta gagcaatcat
    10381 aatcccgcca ccaataacaa atggcaacat ttgtgagaca ccgcccatca agtgtttata
    10441 gaaagcaccg cctaaacttg ttttctcagt tgatactgct gtttcagatt ttccattttt
    10501 agcatgataa gtgtttcctt cattgtttaa aatcaaagaa atcaattcct gacttttctt
    10561 gataccatct gcaacagggc gagcaatgaa ttgtttacca tcaaaacggt ccatttcaac
    10621 cgctttatca gctgcaacaa taactccttt agctctctga atatcttcag cggttaaacg
    10681 attagccaca ccagaagcac cgtttgtttc aactttaata gctactccca tttctgcagc
    10741 ttgttttttc aaagcctctt ctgccatata agtgtgagca attcctgtcg gacaagctgt
    10801 aacagcaacg ataaagtctt gaccacttgt tggagcaacc acttcttctt tagctgttga
    10861 cgcttcatca aaaacagcaa tgactgcttc tggtgtcgct gcagcacgaa gtttatcagc
    10921 aaagccgtct ttcaaaaggt attgtgataa ttctgctagc gcagccaagt gagtatcatt
    10981 tgctccttga ggcgcagcaa tcataaagaa gagatccgtt ggttgaccat ctaaagcctt
    11041 ataatccacg cctttatttg atttcgcaaa caagaccgct ggtttatcta ctacaatatt
    11101 tttagaatga ggcatggcaa tcccatcacc aagccctgtt gatgtttgtt cttcacgtat
    11161 catgatactc tttttaaaga catcaaaatc atgtacaata tctttttcaa ctaatttagt
    11221 aatcatctcg tcaatggcaa cttctttaga aatagcctgt aaatcgagaa tcataatatc
    11281 ttttctcaat aaatcttgaa ttttcatcgt ttttctacct caactttatg atatgtttcc
    11341 ttaataaatg caattgtcgc caagtcatca gagaaagccg tcgctgtacc acaagccacg
    11401 ccccatttta aggcttctat tggattttga ctcttcataa attcgcctgt aaagccagca
    11461 accatcgaat caccagcacc gacagaattt ttaacttctc ccttaatcgg tttagcaaaa
    11521 taagtcgctt ctttagtaac cagcaaagca ccatcaccag ccattgaaat gatgacgttt
    11581 tgagcaccca tctctaaaag acgacgggca taggtttcaa catcatcaag actagtcaga
    11641 atcgtaccaa aaatagcttc tagttcatga ttattaggtt taaccaacag aggattatat
    11701 gtaagcgcat ctattaaagt ctgtccttca aaatcacaaa ccacctgagc accgctttgc
    11761 ctaactaaag gaagtaattc cttataaaca gcattgccaa gattagctgg cgctgaacct
    11821 gcaaagacaa ctgtatcctc tgctgttagc tgggaaagct tagttttcaa ctcctctagt
    11881 tgttcccgac taatgatagg tccttgacca tttaattcgg tctcttcttg tgacttgatt
    11941 ttaacattga ttcgagtatc ttgatcacct tttacaaaat cagttttaac gccttctgca
    12001 ctcaaacttt cttcgataaa acgtcctgta aaaccaccca agaaccctgt cgccgtgcta
    12061 gtaatgtcta aacgttgcaa gatacgactc acattaatgc ctttacctcc agcaaactta
    12121 tcatcactcg ccatgcgatt cactgaccca agatttattt ggtcaattct gacaataaag
    12181 tcaatagaag ggtttaaggt cacggtataa atcataattc aatcaccttt gccttttcct
    12241 ttatttgttt aagaatactt gctgatcctc ctaatgtaat aatagtcaca tcattaatag
    12301 gagctacttt tacaaacgat acctgaccaa ttttagtgcc atctactaaa atgtaagcta
    12361 attttgcatt tgacagaacc gcttttttaa taactgcttc ttccatatca ggagtcgtca
    12421 ggtaagaatc atcaacacca ttcattccaa gaaatgcctt gtcaaaattc atctgcctaa
    12481 tctgttccaa agctacatta ccaatactgg catcggtcgt ttgtttgaca taacccccta
    12541 caataatcgt ctcaatagag agttcaacta atctagcagc atgatgaata gaattagtaa
    12601 ctacagttaa atttttagct tgtaaaaaag gtaataaaaa ttcagtcgtt gtcccagcat
    12661 caataaagat cacatcgttg tcatagataa actgagaggc tctttgcgca atagcttttt
    12721 taatatgact gtttttgaca gacttttctt ggttagaaag ttcctcttgt aaagagtgaa
    12781 aaagttcagc acctccatga acacgatgta gccgtccttc ttgctccaac tctcctagat
    12841 ctctacggat agtagactca gaggagttga gtaattgcat taaatcctct aaagaaacat
    12901 agttttcttc agtgatcttt gccatgatta attgttttcg ttttgatttt aagatagaac
    12961 cacccctaac tgaaatcgtt tacaattatt atactatttt ataactaaaa gtcaagcata
    13021 acctttcaaa atctatcaaa ttctaccaat tattatttct tttattaact cataatctag
    13081 gctcacaaat aatgacgcct aggaaaaata ctatcagcat aacaaaaaac tcaatattaa
    13141 agctttagcc ataatattga gtataacgat ttgatagcgt ttattggatg ttcaacactg
    13201 cttctttagc gtgaactagc tctgtaatca tacggcagta tggagtattg ataccaagct
    13261 tttcaccttt ggtattgact gcaccattga taaaatcaat ttcagtcaat cggtgatttt
    13321 gcacaaggtc ttggtgcatt gatgggtaat ggtgagcagc tttaacagat gtatccatta
    13381 catattgtgt gatttcttct tcgtttagtt tgacaccttc tgcttgacca acgataacaa
    13441 actcatggat gatttctttt accattttga gaccgtcttc actggcaaat aattcaccaa
    13501 ttgtacaatc tagaagggca caagttgagt tcatggtacc attcacgcaa gcttttcgcc
    13561 agatgtttgg cacaacattc tcatcataag tcgcattcaa gttagcttca tttaacaaat
    13621 cagccacttg gtgtccagct tcttgattgc ttggatccat gctttgtaag ttaagtgctc
    13681 ccacaccttg aagatgagca cgaccaggac cttctagacc agcagtccag acagtgaccc
    13741 ccatcaaaat gttgtgctct ggaatgtact gacgaataac gtcttcgtgt ccaagaccat
    13801 ttaaaaggca aagcactttg gtttcttttc caataatgcc tttaatgtct tgaagcattt
    13861 gtggcaactg cattgctttt gtaaacaaga taatcaaatc ggcttcttga gtggcttcag
    13921 tcggtttcat aataggaagc ttaactgttt cttccacatc acctgttaca atcaacccgt
    13981 tttctttaat ggcattgata tgatcttccc agttatctaa caaaatcaca tcgttatttg
    14041 tttttgaaat ttggtaacca aaacggcacc ccatggctcc tgagccagca atataaacta
    14101 acatattttc ctcctattag ccttcaaaga cgaaaatgtc ttctttataa agttttagaa
    14161 tatctttaga aaccttatgc gccgcaaaag caaccgcaaa cggaacaatg atccaagcaa
    14221 ggataataat aagtattgag ctaccacctg ctaaagaagc aatcggacct actgcaccaa
    14281 ctaagccaaa accagatgaa gctggtgttc caacaagatt aaacaatggt actgttaatg
    14341 agctaattgc tgcagtgaac accatcggaa tagccatgat aggatgtttc aagaagtttg
    14401 gcatcatcat tttcatagcc ccaagtgcga tagcaattgg tacaccagat ttattaactt
    14461 taagcgtcgc ccacactaag actgctgctg tcgatgcaat tcccatagaa gctgctcccg
    14521 cagccattcc attaagccca atagctaaac cgattgcaac agttgaaatc ggactaacaa
    14581 tgataagcga gaaagcaact gcaattaaaa tagacattaa aataggttgt aaggttgtaa
    14641 aagaattaat cccttgtccg atcagggttg tcacgtaaga cacataaggc aaaaatttcc
    14701 aaccaatata acctacgcca gttcctatag tgataggtaa caagataatg gttaaagagc
    14761 caaatttatt cccaaaatat ttcacagcta aaaccgcaag gctagctgta atcatcatat
    14821 tgatgagatc cccaatacct ctaagttgga atgttcctgt cgccacacct ttttgaatga
    14881 cttctgtata agcccaggct ccagaaccaa tataggctgc tccaccaaca gccaactgtt
    14941 gcataggatt aaacttaaat tgttgcccaa tcaagaaacc agccataatc ggcgtgaaga
    15001 attggaagac ttgcacaatg tgtaaaaatt cagcagctgc catgtttggc aaaagcggtt
    15061 ttaagaaagt cgctaaaata gcatttggaa tgagtgccac aacgatagca atcgctgtac
    15121 cagccaagac cttattcata aaagaactaa atgtctcttt atctgttgtt gtcatgatag
    15181 accccttttc attgtaatgt gttaatctgt gaaaaccatc acactttttt cacaatgact
    15241 attttatcac aaagtataaa acttgtcacg aagacttagg taaaaaccat ttgtcctctc
    15301 ctgacaaact gaatattagc tgttgctact gctcttgaaa cttccgactc ccttagtcaa
    15361 ttaaagaggt tgaatgcacc acacggttat ctcgatctgg ataaatatag ttaatggcct
    15421 gattaactgc tgttggtgct tcgccaaaac cagtagcaat caaatccact ttcccattat
    15481 aagcggctgc atcaccaatg gcaaaaatac cttcttgact tgtttgaaaa agtggtgaaa
    15541 cagtaatact tgaacgctta tagtcgagat tccaattttt caaattctta ttagaagttg
    15601 aaaaaccaaa actaacaata agactgtcca attcaagttc taacacttca tcttctttca
    15661 ctttttgaat aactaatttt tcagccaggt ttccaattcc tttcaatgct tttggcacat
    15721 aaggtgttaa gaggttgacc gttgatgctt tcaataattc cacactgtgt tcatgtgctc
    15781 tgaaagcatc acgacggtgc acaaccgtca cactctcagc aatatcttct aaagccaaag
    15841 cccaatcaac tgctgaatct ccaccaccac aaatcacaac tttttgtccc gcaaactggt
    15901 ccaactggtg aacattataa aaaaggttat gatcagcaaa gttttcctca ctttctaacc
    15961 ctaaagttct aggtgcaaaa gcgccatttc cacaagcaat gataatggct tttgaaaaat
    16021 gctctgcctt attagtccta atcgaaaaac cacctttaac cttgtcaaag gttaatactt
    16081 cttcttttaa acaaatggtt gtacgatctt caaaacggct taattgtttt attaaatttt
    16141 ctgtcaactc agcacctgtt aaagatggat aagctggaat atcataaatc attttctcag
    16201 gataaagaat ggcaggctgt ccaccaagtt ctgacaaact ctcaataatc tttactgtta
    16261 cgcccctaag acctgcataa aaggctgcaa ataaacctat tggaccacca ccaataatcg
    16321 taatatcata tgctttatct ttcactaatc ttctccttga cccaatgctt ctttaatctt
    16381 atccaatagc ttgcgctctt cctcggaaaa atcgtaccgc tctaacaaat ctggccttct
    16441 caaataggtt tttctgagac tttcttctag acgccacaag cgaatacgtt cgtgatgacc
    16501 actcattaga acatctggaa ctgtcatacc acgataatca taaggtcttg tgtattgagg
    16561 gtattctaaa agtcctgatg aaaaagaatc gtcctgatgg ctactttctt tcccaagtac
    16621 ctgtggaatt aacctgacgg tcgcatccac catagtcatg gccgccaact cacctcccgt
    16681 taatacaaag tcgcctaatg aaatttcatc cgtcaccaag gtcttaatcc gctcatcata
    16741 accttcataa tgaccacaaa taaaaattaa ctcttcctca agagctaatt cttcagcata
    16801 agcctgagta aagggtttac ctgctggatc taaaagaata atccttggtt ttttggcttc
    16861 aatctgttca atggtgtcaa aaatgggctg cgctctcaat aacatgcctt ggcccccacc
    16921 atagggttcg tcatccacat gacgagcttt ttcagcataa tctcgaaagt tatggtagtg
    16981 aatgtctaac agaccttttt cttttgcctt gcctacaatg gaatgctcta gaggggcaaa
    17041 catttcagga aaaagggtta aaatatcaat cttcatcgtc taagccttcc atcaattcaa
    17101 catccacgcg cttgttagga acatctacgt taagaactac tgggggaata tatggtaaga
    17161 gcaagtctcg ttttccttgg cgtttcacta cccaaacatc attagcacct ggctgcaaga
    17221 tttccttgac atagccaatc aaccggtcct tttcataaac agccatgcca ataatctgat
    17281 ggtaataaaa ttctccttct tgtaaatccc cttgattatc ttttgagacc tttaaagtgt
    17341 atcccttgta cttttcaata gcattaatat gatacatgtc tttcaatttg ataatatcaa
    17401 aatgcttttg tttacggtga ctaacaattg tcacttcttg aacaaaccga tctttatcat
    17461 caaataaagc taattgtgag ccttttttaa agcgttcctc agcaaaatca ctgacagaca
    17521 acacgcgcat ctctccttgc aggccttgcg tgttgacaat ttttccaaca ttaaaatatt
    17581 ccatggtagc tcctaaatcg ttaactcttt atctcacgtc actttagcga cctctcttga
    17641 ttctaaagtt tgatagacac ttctttttca gaaccaaatt acctattatt ttatcacgtt
    17701 atagtctatt ttgcacttac agttttgtct ttctctgtta aattgttaaa atacgtctgt
    17761 attaaaaaat tgaatttggc aagatgactc actagtcatt attttatgag ctctaaataa
    17821 tactttaata gttcatttgc agacatcctt tgcctttgat ggagccacca ttttaaggtt
    17881 tctacaaaac tagatagaac aaattgtttc aggaatactt ctggaatagt cgttttgtcg
    17941 atatacttgc agcgcaagtt agggtaaaca tcatgttcta gttcgttttt aaatcttaat
    18001 aaaaaatagg gatcattaga aagtaacaac gtcgcaatgc tgtctttatt ttgttcaaaa
    18061 tgctttaaaa tatgtaccaa atagtcttca aaagtcacat ttcttttttg cctaaacaaa
    18121 tgatgaaaaa gttcttcaca gagttctttt agtaacattt ctttgctttc ataatgtgca
    18181 taaaaggttg atctccctac attagcaagt gtaatcatat ctcgaacagt tattttcgaa
    18241 tattcttttt tttgtaataa agcaataaaa gcactataga tagctgtttt tgtttttttg
    18301 atttgtctgt cttttgtcat atcggacact ttctaaatgt tgttcactat tgaacaggtg
    18361 ctcatttttg gttatagtaa ttgtatttta acttctaga
//
LOCUS       DAUCWO010000033        18087 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUCWO010000033 DAUCWO010000000
VERSION     DAUCWO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18087
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.033"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            180..1739
                     /locus_tag="VGY20_001075"
     CDS             180..1739
                     /locus_tag="VGY20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ4273934.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQI
                     TTKSFIVYQKK"
     gene            1836..4034
                     /gene="nrdD"
                     /locus_tag="VGY20_001076"
     CDS             1836..4034
                     /gene="nrdD"
                     /locus_tag="VGY20_001076"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ4273935.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            4216..4362
                     /locus_tag="VGY20_001077"
     CDS             4216..4362
                     /locus_tag="VGY20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273936.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            4391..5326
                     /locus_tag="VGY20_001078"
     CDS             4391..5326
                     /locus_tag="VGY20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ4273937.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLSFASWDTTLHKRGFETMINAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            5335..5844
                     /locus_tag="VGY20_001079"
     CDS             5335..5844
                     /locus_tag="VGY20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4273938.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     RRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGILEDSRGGTERY
                     WIEEQRGDK"
     gene            5844..6458
                     /gene="nrdG"
                     /locus_tag="VGY20_001080"
     CDS             5844..6458
                     /gene="nrdG"
                     /locus_tag="VGY20_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ4273939.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(6632..7363)
                     /gene="yaaA"
                     /locus_tag="VGY20_001081"
     CDS             complement(6632..7363)
                     /gene="yaaA"
                     /locus_tag="VGY20_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ4273940.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(7405..8262)
                     /locus_tag="VGY20_001082"
     CDS             complement(7405..8262)
                     /locus_tag="VGY20_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4273941.1"
                     /translation="MTSPYNSTISLGTVSLNVTDLAKMTTFYTSIISLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            8449..8786
                     /locus_tag="VGY20_001083"
                     /pseudo
     CDS             8449..8786
                     /locus_tag="VGY20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9065..9778)
                     /gene="treR"
                     /locus_tag="VGY20_001084"
     CDS             complement(9065..9778)
                     /gene="treR"
                     /locus_tag="VGY20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ4273942.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            9989..12013
                     /gene="treP"
                     /locus_tag="VGY20_001085"
     CDS             9989..12013
                     /gene="treP"
                     /locus_tag="VGY20_001085"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ4273943.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLETFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            12081..13709
                     /gene="treC"
                     /locus_tag="VGY20_001086"
     CDS             12081..13709
                     /gene="treC"
                     /locus_tag="VGY20_001086"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ4273944.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKDKSRDNARTPMQWDASEHAGFTTGKPWLEVSKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQSVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILAEN"
     gene            13917..15812
                     /gene="pepO"
                     /locus_tag="VGY20_001087"
     CDS             13917..15812
                     /gene="pepO"
                     /locus_tag="VGY20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ4273945.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGENIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(15979..17019)
                     /locus_tag="VGY20_001088"
     CDS             complement(15979..17019)
                     /locus_tag="VGY20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ4273946.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(17153..17920)
                     /gene="rpsB"
                     /locus_tag="VGY20_001089"
     CDS             complement(17153..17920)
                     /gene="rpsB"
                     /locus_tag="VGY20_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ4273947.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEKGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
BASE COUNT         5270 a         3145 c         3988 g         5684 t
ORIGIN      
        1 taatatcatt tttcaactta ctttgtttat gcactgtcac tttgctatgt gcaatacaaa
       61 gtcatgttat ttatctcata ctaaaaaata atggataata gctatgttag actagtgata
      121 tgaagaaata ttgcttttat tgaatcgtta gagaatatga tatttcaagg agaaaaatag
      181 tgatcgagtt aaacgatgaa ttcatccgaa aagagacgat agaactggcc aacgatggcc
      241 cacgagtaca caccactcaa tatgaaacta aagtacctcg gcttcataaa tgctatctgc
      301 ttttctttag cattattatc agttctttaa caattgcagt acctttttta acagatgcag
      361 ccaatggtct tcagtcccaa aacttatata taggaatgat gttaacgaag gggcaacttc
      421 cctatagtga tgcttttaca acaggagggc tcttttactt tgttatcatt gcattaagtt
      481 attatttggg atcgacactt tggctagtct ttgttcaggt gttttgtttt tacttatctg
      541 gtttatatct ttataaactc attaattata tgacaggctt tcaaaaagtg gctttaactt
      601 tttcaattag ctactatttg ttgtctgtta gtcttggttt tgggggattg tatcctactc
      661 aactggctat gccatttata ttaatatcgg cttggttttt aactaagtat tttgcctgtt
      721 tagtgaaaga tgaggcattt attctttttg gctttgtagg tgctcttgca atgctaattg
      781 acccgagtac ccttatcttt tggtcttttg cttgtgtgac agttttttct tataatataa
      841 gccaaaagca tcttgcaaga ggtttttatc aactgctagc ttcgattttt gggatgattt
      901 tagtttttta cacagcagga tatttcattt tgaacttaca agtgctaaat ccttatttat
      961 cacaaacgat gatttatcct tttacttttt ttaaatcagg aaacttatcg ttgctttttg
     1021 gactggctat tcagttgttc ttcgctttgg ggcttggtct tttgacggga atggagaatg
     1081 tcattaggcg atttaaaaac aattctgata gggtcgtcaa gtggctattt gttatggtca
     1141 ttctagaatc tatacttgtg gctatatttt cacaagacta tcgcccctat catcttttac
     1201 ctcttttacc ttttggatta attttgactg ctattcctgt tggctatcag tatggtatag
     1261 gattaggtca gagtagtcat cgcagacgtc atggtaaaaa tggtgttggt cgagtaatga
     1321 tgatttatct taagagacac ttttatttgc caattttaat tgtagggaca atactaatct
     1381 gttctactta ttgtttcatt agtagtattc ctcttaatca ggagcgtgat catattgcta
     1441 gttatttaga acagaaacta aataaaactc aatctattta tgtttgggat gatacttcta
     1501 aaatttattt ggacagtaaa gctaaatctg tttctcaatt tagttctcct gacatcaata
     1561 cgcaaaaagc gagtcatcga aaaatattag aagatgaact attagaaaat aaggctgctt
     1621 atatcgttgt taatcgctat aaaaacctgc ctaaaatcat tcaaaaagta ttatctacta
     1681 attacaaagt agataaacag ataacgacaa aaagttttat tgtttatcag aaaaagtaat
     1741 ctaatatcaa tatattgtgt tcgagcaaaa aacttgacac aatatattga tttttttagt
     1801 tgtcgtgata taatagttcc gtaaggagat ttgatatggt aagtttagaa gaagacaagg
     1861 tgactgttca acctgatatt aaagtgatta aacgagatgg tcgccttgtt aattttgata
     1921 gtacaaaaat ctatagtgct ttattaaaag caagcatgaa agtaactcgg atgtcgccac
     1981 ttgttgaggc taaattagag gctatttctg atcgcattat agcagaaatt attgagcgtt
     2041 ttccaactaa tatcaaaatt tatgaaatcc aaaatattgt agagcataag cttcttgcag
     2101 ctaatgaata tgctattgca aaagaataca ttaattatcg tactcagcgt gactttgcac
     2161 gttcacaagc aacagatatc aatttttcta ttgataaatt aattaataaa gatcaaacag
     2221 ttgttaatga aaatgctaac aaagatagcg atgtttttaa tactcaacga gatttaactg
     2281 ctggaatcgt agggaaatcg attggtttaa aaatgttacc ttcgcatgtt gctaatgctc
     2341 atcaaaaagg agatatccat taccatgatt tggattacag tccttataca ccgatgacga
     2401 actgctgttt aattgacttt aagggcatgt tagccaatgg ctttaaaatt ggtaatgctg
     2461 aagtggaaag tcccaagtct attcaaactg caacagctca gatttcacag attattgcga
     2521 atgtagcatc aagtcagtac ggcggatgca cagctgatcg cattgacgag tttttagccc
     2581 catatgcgga gcttaacttt aaaaaacata tggctgatgc taagaaatgg atcgttgaga
     2641 ctaagagaga aagctatgct tttgaaaaga ctcaaaaaga tatttatgat gcgatgcagt
     2701 ctttggagta tgaaattaat acgctcttta cgtctaatgg tcaaacacca tttacttctt
     2761 taggatttgg tttggggacg tcttggtttg aacgtgagat tcaaaaagct attttgacca
     2821 ttcggattaa tggtcttggt agtgaacatc gcacggctat tttccctaaa ttaattttca
     2881 cggttaaacg tggcttgaat ttagaaccag attcaccaaa ctatgatatt aagactttgg
     2941 ctttagaatg tgcgactaag cggatgtacc cggatatgtt atcttatgat aaaattattg
     3001 atttgacagg atctttcaaa tctccaatgg gatgccgctc tttccttcaa ggctggaaag
     3061 atgaaaatgg gcaagatgtg acctcaggcc gtatgaatct tggggttgtc accctcaatt
     3121 tacctcgcat tgccatggaa tcaaatggcg atatggataa gttttgggag ctgtttaatg
     3181 agaggatgct aattagtaag gatgctttaa tttatcgtgt cgaacgtgtc acagaagcaa
     3241 aaccagcaaa tgcccctatt ctttatcaat atggtgcttt tggaaagcgt ttggagaaga
     3301 cagggaatgt aaatgatctc tttaagaatc gtcgtgcaac agtctctctt ggctatattg
     3361 gtctttatga agtggcgtct gttttttatg gtggtcaatg ggaaggtaat ccagatgcta
     3421 aagcttttac cttgtcaatt gtcaaggcaa tgaaacaggc ctgtgaggat tggtcagatg
     3481 aatatggtta tcatttctct gtttattcga ctccatcaga aagtttgaca gatcgctttt
     3541 gtcgtttaga tacggaaaaa tttggcattg tgacagatat tacggataaa gaatactata
     3601 caaattcttt tcactatgat gtgcgtaaga gtccgacacc ttttgaaaaa ttagattttg
     3661 aaaaagatta tccagaagca ggtgcttcag gtggttttat ccactactgt gagtatcctg
     3721 ttttgcaaca aaatccaaag gccttggaag cggtttggga ctatgcttat gatcgtgtgg
     3781 ggtatttggg aaccaatacg cctattgata aatgctataa ttgccaattt gaaggtgatt
     3841 ttaccccaac agaacgtggt tttacttgcc caaactgtgg caataatgac cctaaaacag
     3901 ttgatgtggt caaacgtaca tgtggttact tggggaatcc tcaggcccgc ccaatggtta
     3961 acggtcgcca taaggaaatc tctgcgcgtg taaaacatat gaatggttct actataaaat
     4021 acccaggcct gtaaactcaa gctgggggag agcccttttg atggaaatat gatatgctta
     4081 aaaggttgct tcatgcaagc aatcttttat tgtttaatgg tttcttagca attgtgagat
     4141 aagaataact aatccaaaac gactaattag ctgttagcaa tcggactata agaagaaaga
     4201 gataaggaag aaataatggg aaaatatcaa ttagactata aaggaatgca gcaggtagaa
     4261 cgctttcatg agaagcattc taaaaaaaag acggataaaa aatcccgcgt tcaagaactt
     4321 aaggctcggt ttttagagaa gtcaaaaaaa caggataact aaagtatcca ataaagaaag
     4381 aaggttgagt atgcttaata ttggtattgt gggactaggt gccatttcac aaaaagccta
     4441 tttaccatat atgagacagc tgagtgacat tacttggcat ctatcaacgc ggaatgcagc
     4501 agtacgtcag caggtcggtc agttatttgg tcacgctatt ctttacagtg atgtcaagga
     4561 gttgtcaaaa acaaacttag atggggtctt tattcatgcg gccacatcgg cccacgcaga
     4621 gttggctagt ttatttttaa atcaggggat cccagtcttt atggataaac ctatagctga
     4681 caattacctg atgacaaaga acctttatga cttagccaaa gaaaatcaga cttttctgat
     4741 ggcaggattt aacaggcgtt ttacacctcg tgtcaagaag ttgtcaagtt tgtcaactaa
     4801 gcgtaaagtg gctgttgaaa agaatgactt gaaccggcca ggagatatga cttttaagct
     4861 ctttgatttc tttatccatc ctttagatac agccttattc ttgacagaag ggaccttact
     4921 aaaagggcat tttcaatatc atctggaagc aggcttactg agtcaagtta tggtaacctt
     4981 gatgaccgaa agcatgacga ctacagcttc tatgaattta caatcaggga gtcgccgtga
     5041 ggtaatggaa gttcaacggg ctgaagaaac ttatcattta gagaacttag acgagttatc
     5101 tatttataaa ggtactgaaa aaagggtgct tagctttgct tcttgggaca ctaccttgca
     5161 caaaagaggt tttgaaacaa tgatcaatgc ctttttagaa gctattagca caggcgtcaa
     5221 tcctgtcagc cctgagtcta gtcttttaag ccactggatt tgtcaacaga ttgctgactc
     5281 tcagctttct tatggagagt taacggtaga gttgcctaag gattaggaat tgatatggaa
     5341 ataagacgac cgactttaaa agataaagac gctgtattgt caatgattaa cgaattttta
     5401 gagcaaaaaa gtgctacaga tgggttatgg cattttaacg tcaatgactt taactatgaa
     5461 acatggttgg aagattcctt gcgacaggaa atgggtttgt ccagtcaagg agtgcctgct
     5521 attcaatacg tcgcatttga tgagagaagt caggctatcg ggttcttaaa tcttcgttta
     5581 cggttaaatg agagattact tgaaaaaggt gggcatattg gttactctgt ccgtcccagc
     5641 cggcgtggga aaggttatgc caaggagatg cttaaacaag ctgtcagcta tgcgatttcc
     5701 aagaatatca cgacaatttt ggtaacttgt gatgagacta atgttgcaag ccgagcagtt
     5761 attgtggcta acggcggtat cttggaagat agtcgaggtg gaacggaacg ttattggatt
     5821 gaagaacaaa gaggcgataa gtgatggcag aaaagtgttg gaataatcct aagccaaagg
     5881 aatggcaggc agaggagtta agtcaaggcc ggattattga ctataaggct tttaactttg
     5941 ttgatggtga aggggttcgt aattcccttt atgtgtctgg ttgtttattc cattgcaagg
     6001 ggtgttataa tgctgcgact tggtctttta aggcgggaat gccttatacg caagaacttg
     6061 aagagcagat aatgacggat ttggcacagc cttatgttca gggactgaca cttttaggtg
     6121 gagaaccctt tttgaatacg ggtatcttaa ttcctctgat taagcgtatt cggcgtgaat
     6181 tgccagaaaa agatatttgg tcttggacgg gttatacttg ggaagaaatg atgcttgaaa
     6241 caccagacaa actggaaatg ttgtccttaa ttgatattct ggtagatggt cgttttgata
     6301 tcacaaaaaa gaacctcatg ttgcaattta gaggctcttc taaccagcga attatcgatg
     6361 ttcaaaagtc tttggctgct aaagaagtga ttatctggga taagttaaac gatggggacc
     6421 aaacctttga acaaattagt cgggaggatt tgctttaaga agctgactta tccacatgat
     6481 ttttttgaaa aggtcatgtg aaaacaggtc ctttaagggt gaattgggga gaaagtaatt
     6541 gttatttcct ggttttcccc acattatcct catagttatc cacaggttgt gtataagttg
     6601 tagaattgtg aataaagact aaaaaagagg cctaggcctc ttttttgata tagacaaatt
     6661 catgatctgt tgataggtct ggatggtagt aaaaaccagc aaagacaagg cttttaaggc
     6721 tgtcaacagt ttggcaatta ttttccatac aggcagtgag aaatgcacct cgtgcctttt
     6781 ttgaaatggt ggaatgggtt ttgaattggc cctctttttc tgccataaac ttgggactta
     6841 tccacaattg tttacagtct ttggaaaaga catcgtcaaa ttcactggat aaaagggaaa
     6901 tcacttgggg atgctctttg gcaaactgat tataacaggg ccgccagtaa gacttgaggc
     6961 tttgtccttc aatttttatt ctggtgtgaa aatcatggcg gtgctctgcg ataggatgat
     7021 tagcgggaat gataccgtaa aaggatgagg tgatgtaaac ttgctgggtg aggtaagcct
     7081 gttcttgggt agtgagctta tcgcgcttga tatggcggta cataaggcca ttgaacagtt
     7141 ggtaggcagg gtaagccaag ctttgttgag aggccatgtc ttgccagcgt tgctgttctt
     7201 ttttagctgc ctcttctttg atacgatagg atttggccaa gtcctccgtg gtcatagctg
     7261 ccatgatttt taaaatggct tggctgtctt gtggaaggag gtgtgggtga gattccttag
     7321 ggatcatcat ttcttttgcg gttggaatta aaaaggttag catagtgtta ttgtagtaaa
     7381 gttacttgct ttctgtcaag aaaactagtc ttgtccaagg atgacgttca cacagatacc
     7441 ttcctcgtct tctgtcgtga aagaagaaga atcttcttgt aaaatagcta ggccgtgtaa
     7501 tcttgctttt ttcagagtag ctgaaaaaag caggggcgtc tcaatgtgaa tggtcaagaa
     7561 ggctaagcct ggggcacctt cttggtgttt tttgaggtaa ggggctgacc agtgattgaa
     7621 tgcaaggtga tgatggtagt tccctgaagc aatccagcta gcacttggaa tggtcatttt
     7681 atcgcctaaa tcaaaaacct tttgatagag aagtgaagaa gccagagcat ttttgacact
     7741 cagatggaca tggccaatgc gtgtgtcttg agctagtaaa aagcgcttgg ggatatctgt
     7801 caactgctcc aatatgcttt tggtatccgt gggctcagtc acaccaataa tttgaccatt
     7861 gtcgcggata tcccaatgct caacagcttt gtcatgataa atttcaatcc cattgccctc
     7921 aggaccgctg aggtagatag cttcgctata gccgtgatca gctgatcctt ctaaagagat
     7981 acctctggtt aagaaatgat tgagaacaag tcccaagcta tgacgatcag gaactaaaaa
     8041 ggctgtgtga taaagaccat aagccttatt tgctggtaaa ggtgtttgtc gtaactctaa
     8101 gataacggtt ttaccgtctg tagtcaattg acgagatgtc gtgtcttgtg agagaacctg
     8161 taaactgata atacttgtgt aaaaggttgt catctttgct agatcggtaa catttagaga
     8221 aaccgttcct aaagagatgg ttgagttgta aggggatgtc atcctattac ctccaaaaac
     8281 taattaaatc tatcataatc aatcagtttc aaaaagaaaa gtaggcacaa ataagtggta
     8341 taggcactaa aaagtaactc atttgttaac cctatcttgt ttctgttctc tctaatagtc
     8401 tataatgggt tataagttac aaaaagatac taagaggagc tgctgcctat gctgaaaaaa
     8461 agaccggctt gtcccgtaga aacggcctta tcggtaatcg ggaataaata gaaattatta
     8521 atattaagag acttgctaaa gggaccttgc gttttgggca gttgaagtcc tcaataggtt
     8581 tggtcagtca aaaggtactt actgcacaat taagggctat ggaagcagac ggattggttc
     8641 atcgggaagt gtatgcagaa gtgccacctc gggtggagta ttccttaaca gaaactgggt
     8701 tgagcttagc tcctgttatt gaagcgatgt cggattgggg ccaaacttac caagaaaaac
     8761 atgccgattt atttgaagaa ggctagagga aagagttgtc cgagcatact gagacttaga
     8821 gaagctgata gtttttaaaa aatgatatta tcaaattctt tacagtttga taaaattact
     8881 ttttttctat aaaaagactt gcgctcatct cacgaatatg ataatataat atcaagtctg
     8941 tttgagaaaa cagaagctca ggtcaagtaa gtgcacactt acaataacac agttgataat
     9001 ggggaaactc agagtcaaga aaaagaaaag caagccttgc attttgcaag gcttttttct
     9061 gtctttattt tggtctgcgc cgagcaaaat ctaaaaattt aaatttttcc agtttatggc
     9121 gactctcggt gaattggaat tggttattat tggaaaggta gactttggat ttgacggaaa
     9181 caacatgttg gtctgatcca aggtcaagta aaattttatc acgatcggtt atttggtcaa
     9241 tggtaacctc tttgagggca aagtcaatgg cgagatggag ttccttttcc aaatattgat
     9301 aaatagaatg ctctgcgatt tcccttgtca ttattggcac caaagtttta ctaaggtaat
     9361 caatgtctaa gacggaagcc acaccctcaa caacgcgttg gcgtgtaaca cgccagacca
     9421 agctgttttt gctaaaaccg gtcaattttg aaagtgtctc atccacaatc agtttatcaa
     9481 tggcaatgac gttggtctta gaatctagat ttgagtaaga gacgagttcc tgataactag
     9541 ttagttctga gatagggaac atgatctgat gatgtttaat cacttgggtg ccacgtcctt
     9601 gttttttgag gataagacct gcctttgtga gaagtgagag agccttgcgg acggtatctc
     9661 ggctagcttg gtattgttga ctcaattcta tttcagtagg taaaaaatcc ccttctttgt
     9721 aaaagtcttt gtttattttt gtttcgaggt ctttataaat acgttcatac tttgtcatag
     9781 ggtaaattac ctttccattt gtctattttt ttacagacaa caccttttat tttaccaaaa
     9841 taatggcttt tttggcacaa acaacttgca gacaaatttg caatcgatta caataaaggt
     9901 gtcaatcata ttttatctaa gaatttgaat atttcatttt gacgttgctt gttaaaagca
     9961 actagaacaa aggactaggg gatgtcacat gggaaaattt gaacaggatg ctaaaagtct
    10021 tctaactgct attggcggta aagaaaacat caaggctgtt acacactgtg caacgcgtat
    10081 gcgttttgtt ttgaatgata ataataaggc aaatgtcaaa gagattgaaa aaatctctgt
    10141 agttaaaggg acatttacca atgctgggca gtttcaggta atcattggta atgatgttcc
    10201 agttttttat aatgacttta cagctgtttc tggtattgaa ggggtgtcta aagaagctgc
    10261 caaatcagca gctaaaagta atcaaaatgc cttacaacgg gtgatgacca tgttggctga
    10321 gattttcaca cctattattc cggcgattat tgttgggggg cttattttag gtttccgtaa
    10381 tattttggag agtgtgcctt ttgaatttct tgggcagcag gtcgaaaaag ggaaattagt
    10441 ttttgatgca gctggggatc ctgtttggaa tacgattgtg agggtatctc ctttctggtc
    10501 aggggttaac catttcttgt ggttaccagg ggaagctatt tttcacttct taccagttgg
    10561 gattacttgg tctgtgacgc gtaagatggg aaccactcaa attttaggga ttgtccttgg
    10621 tatctgtttg gtgtcaccac aattattgaa tgcttatgcg gtggcaggaa cgcctgctgc
    10681 tgagattgcc aaaaactggg tttgggattt tggtttcttt accattaatc gtattgggta
    10741 tcaggcacag gttattccag cccttttagc tggtctgtcc cttgcttatc ttgaaacttt
    10801 ctggcgtaaa cggattccag aagtggtttc aatgattttt gtgccattcc tttctttgat
    10861 tccagctttg attttagcgc atacggtatt ggggccaatc ggttggacta ttggtaaagg
    10921 gatttccttt gttgtgttag ctggattgac tggtcctgtt aaatggctat tcggtgctat
    10981 ctttggtgcc ttgtatgctc cgctagttat tactggttta catcacatga caaatgccat
    11041 tgatacccaa ttaattgctg atactgcaac tcgtacaact ggtttgtggc caatgattgc
    11101 tctttcaaat atcgctcaag ggtcagccgt ttttgcttac tatttaatga atcgtcatga
    11161 agaacgtgag gctgaaatat cgcttcctgc agcaatttct gcttaccttg gggtaactga
    11221 gcctgcctta tttggggtta atgttaaata tgtttatccc tttgtagccg gaatgattgg
    11281 ctcaggtatt gcgggtctct tatcaacaac ctttaatgtt caggcaaatt ctattggtgt
    11341 tggtggctta ccaggtttca tggctatcaa tgtgaagtat atgattccat tcttcatctg
    11401 tatggcagta gccattgtgg taccgatgtt tttaaccttc tttttccgta aatcacatat
    11461 catgactaag acagaagatg aggctaaact acctgagaca cccgtttcgg aggctcctgc
    11521 agcaactgct ccacataaga ctatgcaagg gacagttatc actttaacaa gccctttaac
    11581 gggtgaagtt aaagcgttgt ctgaagctgt tgatcctgtc tttgcacagg gagttatggg
    11641 tcaaggtgct cttcttcaac cgacagaagg ggtgttagta gcgccttgtg atgctgaagt
    11701 atcggtcttg ttcccaacta aacacgctat ttgtttggtg acgactgaag gtttggaatt
    11761 attgatgcat attggcatgg acacggttaa cttagatggt caaggatttg aagctttggt
    11821 gaagcaaggt gatcaggtta aggctggaca aacattgatt caatttgata tagcagcaat
    11881 ttctgaagct ggatacgcca ctgaaacgcc tcttgtggtg actaatcaag atgcttttac
    11941 ggtaactgtt gaaggtagtt taccgcgtca gattaaggct aatgataagt tagcagtagc
    12001 gatgaaaaag taggtttact agggagaaaa cgttcaggaa ctgagatcag ttcctgaaac
    12061 tattttaggg aggaaaactc atgacaattg ataaaaagaa agtcgtctat caaatttacc
    12121 caaaatccta taaggacacg actggaaatg gtgtgggaga cttgcgaggg atcattgata
    12181 aattgcctta cttacaagaa ctaggaatag atatgatttg gttgaaccct ttctacccta
    12241 gtccacaacg agacaatggc tatgatgttt cagattatac ggcggttaac cctgattttg
    12301 ggactatggc tgattttgaa gatttggtaa aagctgctaa ggagcatcag attgagttaa
    12361 tgttggatat ggttttgaat cactgttcca cagaccacga gtggttccaa aaagctttag
    12421 caggagaccc ttattatcag gatttcttta tcttgagaga tcagccgact gattgggttt
    12481 ccaaatttgg tgggaatgct tgggcgcctt ttggagatac aggcaaatac tacttacact
    12541 tgtttgatgt gacacaggct gacttgaatt ggcggaaccc acatgttcgt gaggaattgg
    12601 ctaaagtggt taatttttgg cgagataaag gagtgaaggg gttccggttt gatgtgatta
    12661 atctgattgg gaaagatgaa gagctggtgg attgtccagt caatgatggt aagccagctt
    12721 atacggatcg tcctattact cacacttatc ttcatgatct caatcaagcc agttttggtc
    12781 aggatgattc gtttatgaca gtaggggaaa tgtctgccac gactattgac aactgtcttt
    12841 tatacacggc tcccgaacgt gaggagctat ccatggcttt taatttccac catctaaaag
    12901 ttgattatga gaacggtcag aaatggacta ttatggcttt tgattttgca gcgctgcgag
    12961 acttattcca tgcttggggt gaaggcatga gtcaaggcaa tgggtggaat gccttgttct
    13021 acaataacca tgatcaacca cgtgccctga atcgttttgt tgatgtaaca catttccgaa
    13081 acgaaggtgc gacgatgtta gcggcttcca tccatttgtc acgaggaacg ccttacattt
    13141 atatgggtga ggagattggc atgcttgatc cagactttga tagtatggat gattatgtgg
    13201 atgtggaaag tctcaatgct tactcaagct tactagtctc aggtaaaagt gcagaagaag
    13261 cctttgccat tatcaaggat aagtcaaggg acaatgccag aacaccaatg caatgggatg
    13321 ctagtgaaca tgctggcttt acgactggta agccttggtt agaggttagc aaatcttatc
    13381 gagacatcaa tgtcgaaaca gaaaaagagg gacgtatttt tcctttctac caacgcttga
    13441 ttgctttgcg gaaggaactc cctattattg ctgaagggga ttatcgggct gcttttaaag
    13501 atagtcagtc tgtctacgcc tttgaacgcc atttaggtga ccagtgtttg cttgttctca
    13561 atcatttcta tgctgatgag gtcgaactgg aattacctcc acgttatcaa catggacagg
    13621 tcttaatcag caactatgag aaagtttcta tttgtgaaaa agtgatactg aaaccttatc
    13681 agacacttgc tatcttagct gaaaactaac taaaagcctg aggttaggcc tcaggctttt
    13741 ggtgattgtc acgaaaaaag aagttttctc atatatttat ttttaaactc ttattttttc
    13801 taaataaaaa caattgagat aagttatttt agctaaattt tggtacaatg gaaaagatta
    13861 taagtagagt ttaagataaa gattctacaa atttaaaatg atataaggag atacgaatga
    13921 caacttatca agatgatttt taccaggctg tgaatggaaa gtgggctgaa acagcagtta
    13981 ttccagacga caagcctcga acaggtggat tttcagactt agcagatgaa attgaggcat
    14041 taatgctaga cactacagat gcttggctag ctggagaaaa tatccctgat gatgctattt
    14101 taaaaaattt tgtgaaattc catcgtttgg tggctgacta cgctaaacgt gatgaagttg
    14161 gagtaagtcc aattctccct ttaattgaag aatatcagtc cttaaaatct ttttcagaat
    14221 ttgttgctaa tatagcgaaa tatgaattgg caggtttacc aaatgaattt ccctttagtg
    14281 tggcaccaga ctttatgaat gctcagctga atgtgctttg ggcagaagca ccaagcattc
    14341 ttttgccaga tacaacatac tacgaagaag gcaacgaaaa agctgaggaa ttgcgtggta
    14401 tttggcgtca gtcgcaagaa aagcttttac ctcaatttgg tttttcaaca gaagaaatca
    14461 aggatctttt ggataaggta attgagctag ataagcaatt ggccaagtat gtcttgtctc
    14521 gtgaggaagg ctcagaatat gccaaattat accatcctta tgtgtgggct gattttaaaa
    14581 aactagctcc agagcttcct ttggatagca tttttgaaaa gattttaggc caagtacctg
    14641 acaaggttat tgttcctgag gaacgttttt ggacagaatt tgcagccact tattactctg
    14701 aagccaattg ggatttactc aaggctaatt tgattgtgga cgcggctaat gcttacaatg
    14761 cttacttgac ggatgatatt cgtgtggaat caggcgctta ttcgcgcgct ctttcaggaa
    14821 cacctcaagc tatggacaaa caaaaagcag ccttttattt ggcgcaagga ccgtttagcc
    14881 aagcgcttgg cttgtggtat gctggtcaaa aattctcccc agaagctaag gctgatgtgg
    14941 aaagtaaggt agcacgcatg attgaggttt ataagtctcg tctagaaacg gctgattggt
    15001 tagcaccagc cacacgtgaa aaagccatta ctaaattaaa tgttatcacg ccacatattg
    15061 gttacccaga aaaattacca gaaacctatg ctaaaaaagt gattgatgag agcttgtcat
    15121 tggttgaaaa tgcacagaac ttggctaaaa tcacgattgc tcacacttgg agtaaatgga
    15181 acaagccagt tgaccgtagt gagtggcata tgccagccca cttagtcaat gcttattatg
    15241 atcctcagca aaatcagatt gtctttcctg cggcaatctt acaagagcca ttttattctt
    15301 tagatcagag ttcttcagcc aattatggag gaattggtgc agtcattgcg catgagattt
    15361 cacatgcctt tgacaccaat ggtgcttcct ttgatgaaca tggtagccta aatgattggt
    15421 ggacacaaga agactatgct gcctttaaag agcgtacaga taagattgtg gcgcagtttg
    15481 acggcttaga gtcacatggt gctaaggtca acggtaaatt aacagtttca gaaaatgtcg
    15541 ctgatctagg tggggtagct tgtgctttag aagcggcaca atcagaagaa gacttctcag
    15601 cacgtgactt ctttattaac tttgcgacga tttggcgtat gaaggcccgt gaggaataca
    15661 tgcaaatgct agctagcatt gatgttcatg ctccgggtga actgcgaaca aatgtcacct
    15721 taaccaactt tgatgctttc catgagacct ttgatattaa agaaggcgat gctatgtgga
    15781 gagctccgaa agaccgtgtg attatttggt aattaggtag ttgataaacc ttcaaatgct
    15841 ttgtgattat aaaaatgact gattctgtga aacacaggat cagttttttg cttccttatt
    15901 ggtgtccgtt ttgacacaac aaaaagacaa ccctcaggaa agaaggttgc cttttttaga
    15961 cttatcagaa ataagtgatt agttgttaag ggcagcagcc atagtagctg caacttctga
    16021 ttcaaagtcg ttagcttttt tctcgatacc ttcaccaact tcaaaacgtg caaaagcaat
    16081 cgctttagcg tttactgagt caaggtaagc ttcaacagtt ttgctgtcgt ccatgatata
    16141 aacttgtgca agaagagtgt aagcttggtc aactttagtg ttgtcaagca tgaaacgatc
    16201 cattttacct gggatgattt tgtcccagat cttttctggt ttgccttcag cagcaagttc
    16261 agctttgatg tctgcttcag cagcagtaat cacgtcgtct gataattgag cttttgaacc
    16321 gtatttcaag aatggaagtg ctggtttgtc aaccattgca cgcgattcgt tgtcaagttc
    16381 gatagcgtgg ttcaattgag caagttcatc tttgatgaat tgtgcatcaa gctcagtgta
    16441 tgaaagaaca gttggtttca ttgcagcgat atgcattgat acttgtttag caagagcatc
    16501 gtcgccgcct tcgacaactg agataacccc gatacggcca ccattatgtt ggtaagcacc
    16561 gaagtgttgc tcatctgttt tttcaatcaa agcaaaacga cggaatgaga ttttttctcc
    16621 gatggtagca gttgcattaa cgtaagcttc agcaagagtt tcacctgaag gcataacaag
    16681 tgcaagtgct tcatcgttgt tagctggttt gccttcagcg ataactttag cagttgcgtt
    16741 taccaattca acgaattgag catttttagc aacaaagtct gtttcagcgt taacttcaac
    16801 aacagcagca acgttaccat gaacgtaaac gcctgttaaa ccttcagcgg caacacggtc
    16861 agctttttta gctgctttag ccattccttt ttcacgaaga agttcaacgg ctttgtccat
    16921 gtcaccatct gtttcaacaa gtgctttttt agcgtccata acgccagcac cagatttttc
    16981 acgtaattct tttacaagct tagctgtaat ttctgccatg tgtagtatcc tccaaatgat
    17041 atttgttttg gtttcaaaaa acgagacaga gcggtttgct ttctgccccg tttggttttc
    17101 gattgttaaa gacaaacaaa gtttgccata agagttagga actctgtgcc aattaagcgt
    17161 tgtcgccttc gacaacttca acgatttctt cgattgaatc tgcttgagtg tcagcttcaa
    17221 aagcaacgtc tgcatcttca ccttgacgac cttcgataat agcgtcagct aatttagcag
    17281 tgattaattt aacggcgcgg atagcgtcat cgttagctgg aatgatgata tcgatatcat
    17341 ctggatcagc gtttgtatca accatcgcta cgactgggat accaagtttt ttagcttctt
    17401 taacagcaat ttgttcttta tgtgggtcaa caacatacat cacgtctggg atacgaggca
    17461 tatcttcgat accgcccaag aatttttcaa gacgagcgcg ttgtttgtta agaagtgcaa
    17521 cttctttctt aggaagaacg tcaaaggttc ctttttcttc catacgtttg atttctttca
    17581 aacgagcgat acgtttttgg atagttcccc agttggtaag cgttccaccc aaccaacggt
    17641 ggttgatgaa gtattggcct gcacgcgttg cttcgtcagc aactgcttca gcagcttgtt
    17701 ttttagtacc aacgaacaag atgacagcat cgtttgctgc agcatcacga acaaattcgt
    17761 aagcttggtc agctaatttt acagtttgtt gaagatcgat aacgtggata ccgttacgct
    17821 ctgtgaagat gtatttagcc atcttagggt tccagcgacg agtttggtga ccaaagtgaa
    17881 caccagcctc aagaagttgt ttcattgaaa ttactgccat gagtagtttc tcctttataa
    17941 aatgtttttt cctcttccag acttcgactt gcaggctaac ctagcggcaa caagcccaca
    18001 attggtctag aatgagtatt tgccgtctac acgaccctac tatattatca aaaaaagagg
    18061 ttaaaagcaa ggagaaatgc tatgaat
//
LOCUS       DAUCWO010000034        16616 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUCWO010000034 DAUCWO010000000
VERSION     DAUCWO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16616
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.034"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(42..1661)
                     /locus_tag="VGY20_001090"
     CDS             complement(42..1661)
                     /locus_tag="VGY20_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4273948.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGSYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(1968..3512)
                     /locus_tag="VGY20_001091"
     CDS             complement(1968..3512)
                     /locus_tag="VGY20_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ4273949.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGVVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(3760..4455)
                     /locus_tag="VGY20_001092"
     CDS             complement(3760..4455)
                     /locus_tag="VGY20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ4273950.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLALAFSKEK"
     gene            complement(4559..5926)
                     /locus_tag="VGY20_001093"
     CDS             complement(4559..5926)
                     /locus_tag="VGY20_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ4273951.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKLHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMISAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHMGDIHLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMDSNGILIAP
                     GDSIIDPYLPENQMVIRFGDHQELNVTDIREEKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVADEDIIEALESVQLTANRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGPDSVALHSQLIKQLSPDRIDQLVFYGDDIQE
                     LAQLAHEFYPEGHVQFFQKNQQEDQFEDMVRQVQQLLAPSDQILLKGSNSMGLAKLVN
                     HLVEN"
     gene            complement(6125..7171)
                     /locus_tag="VGY20_001094"
     CDS             complement(6125..7171)
                     /locus_tag="VGY20_001094"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ4273952.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIRTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEIFDQRESHLI"
     gene            complement(7272..7868)
                     /gene="recR"
                     /locus_tag="VGY20_001095"
     CDS             complement(7272..7868)
                     /gene="recR"
                     /locus_tag="VGY20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ4273953.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYRG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(8657..9436)
                     /locus_tag="VGY20_001096"
     CDS             complement(8657..9436)
                     /locus_tag="VGY20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ4273954.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(9560..10102)
                     /locus_tag="VGY20_001097"
     CDS             complement(9560..10102)
                     /locus_tag="VGY20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273955.1"
                     /translation="MIKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            complement(10263..10784)
                     /locus_tag="VGY20_001098"
     CDS             complement(10263..10784)
                     /locus_tag="VGY20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ4273956.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            complement(10891..11586)
                     /locus_tag="VGY20_001099"
     CDS             complement(10891..11586)
                     /locus_tag="VGY20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ4273957.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            11825..12760
                     /locus_tag="VGY20_001100"
     CDS             11825..12760
                     /locus_tag="VGY20_001100"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ4273958.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLKVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            complement(13060..14922)
                     /gene="cadA"
                     /locus_tag="VGY20_001101"
     CDS             complement(13060..14922)
                     /gene="cadA"
                     /locus_tag="VGY20_001101"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ4273959.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQLASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKAFLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKSFILEMVPDLSAFEETIQVEESQGKTLIFVSRDNQLVAYYALLDDIKL
                     ESKRAIEALHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(15454..15636)
                     /locus_tag="VGY20_001102"
     CDS             complement(15454..15636)
                     /locus_tag="VGY20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ4273960.1"
                     /translation="MLTYDEFKQAIDNGYITADTVMIVRKNGQIFDYVLPNEKIKNGE
                     IVTDEKVEEVLVELSR"
     gene            15876..>16616
                     /gene="mf2"
                     /locus_tag="VGY20_001103"
     CDS             15876..>16616
                     /gene="mf2"
                     /locus_tag="VGY20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ4273961.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGT"
BASE COUNT         5223 a         3403 c         2835 g         5155 t
ORIGIN      
        1 gaaaattaaa aagagctgac tctcatcaac tctctttaag cttattcacc tttgtgacga
       61 atcacaaaac cagtattttt agcacttgat tttttctctt tattgaaaaa gtctttacta
      121 ttacgttttg atttacgttt gaaatcgcgg ttgccactgc catcgtgacc atcacgctta
      181 tcacgttttt gacgacgacg gtcaccatca cgctcatctc tgttacggtc accacgatag
      241 ctgccacggc gatcaccacg gttacggttg tcacgtccac gaccaccttt gccattttta
      301 ttgccgtgac caccacccac atatttgaat ggtaatggtt tttcacgtgc aatttcaact
      361 tccggtaagc tatctggatc ttgaactgtt aagctcaaaa tatataaagc taattcttca
      421 ggagtgaatt cggcagccaa ttggacagca tcacctttga atttatcaaa gtttgaacga
      481 atagtctcat ccgcaaagtc gcgttcaatt ttcttgaggg ctactttttt cttggcttga
      541 aaggcctctt cagcagttgc tgggcgaaga ggtttcatct gttttttcgt taagttctca
      601 atcattgaga ggtagcccat ctcatttggt gagacaaaag tgatagactc accagattta
      661 ccggcacgac ctgtacgacc aatacggtga acataacttt ctggatcttg ggtaatatcg
      721 tagttataaa cgtgggtaac tcctgaaata tcaaggccac gcgctgctac atcagtggca
      781 acaagaatat caatttggtc gtttttgaaa tcacggataa cacgtaagcg tttattttgg
      841 tcaagatcac catggattcc ttcggcacgg aaaccacgaa gtttcaagcc acgtgtaatt
      901 tcatcaactc ggcgcttagt ccgcccaaaa acgatggata attctggctg gttaacatcc
      961 atcagacggg tcatagtatc aaatttttct tgttctttca cacgaacata gtattgatca
     1021 acattgacgt ttgttaattc tttgttctta atttgaacat gttcagggtc tttcatgaat
     1081 ttaaccccga tttgtttaat aggtgccggc atcgttgcgg agaagagcaa ggtttgacgg
     1141 tctgctggga cacgactaat aatagcttca atgtcttcca agaatcccat gttaagcatt
     1201 tcatctgctt catcaaggat taaggtttcc acatgatcta aaataagagc cttgcgctta
     1261 atcaaatcta acaagcgacc cggtgttccc acgacaatgt gggcacctga tttaagggct
     1321 ttgatttgtt tttcaatact tgatccaccg taaacagagc gaaccttaac ccctttttca
     1381 cgtccaaaac ggaacaattc ttcctgactt tgtacagcca attcacgcgt tggtgcaatg
     1441 accaatgctt ggatgatatt ttcgttagta cgaatcttat tcaaggttgg tagaccaaac
     1501 gctgctgttt taccagttcc tgtttgtgct tgaccaataa catccttccc ttctagtgcg
     1561 agaggaatcg tcatttcctg gataggagat gctttctcaa aaccagcggt aacaacagct
     1621 gattggatgt cttgtgataa gttaaattct gtaaatttca attgtttctc ttttctaaaa
     1681 aggcagtgcg aagctgcctt ataaaccttt taattttcgt cgtttgatta acaacctaac
     1741 tagtgtaaca caattagaat caaaaagata ggaaataagg ctaatataag caataacacg
     1801 ctttcacgat tatccgctaa atcataaata tgcttacagt tgtgaagata tcgttttttg
     1861 atagaatctt aaatctgcaa aagagataac tagccactca tttcttttaa aaagttactc
     1921 agtaaattga tctccaaaac gttagactgt tgaaaatcat gtttcggtta cattttctct
     1981 tctaaagtga catctgggta tttgtctgca aaccagcgaa gggcaaaatc attttcaaat
     2041 aggaaaactg gttggtcaaa acgatcctta gctaaaatgt ttcggcttga ggacatgcgt
     2101 tgatccaaat catcttctga aatccagcga acagtctttt tacccattgg tgtcatcaca
     2161 acttcagcgt tgtattcacc ttccatacga tgtttaaaga cttcaaactg cagttgaccc
     2221 acaacaccca gcatgtattc gcctgtctga taattcttat aaagctgaat tgctccttct
     2281 tgtactaatt gttcaatacc tttgtggaag gatttctgct tcataacatt tttcggagaa
     2341 accttcatga agatttctgg ggtaaaagtt ggtaagggtt caaattcaaa tttatttttc
     2401 ccaactgtta aagtatcccc aacttgataa gttcctgtat cataaacccc gataatatcc
     2461 ccagcaacag cattcgtcac attttcccgt gattctgcca taaattgagt aacattggag
     2521 agcttagcgc ctttgccagt gcgtgtcaaa ttaactccca taccgcgctc aaattcacca
     2581 gaaacaatac gcacaaaagc aatacggtca cggtgtttag ggtccatgtt agcttgaatt
     2641 ttaaagacaa aacctgaaaa atctttagct aaggggtcaa caacattacc ttctgtagtc
     2701 ttgtgaccat gaggttctgg ggcaaactcc aagaaagtat caagaaaagt ctgaactcca
     2761 aagttcgtaa gggcagagcc aaaaaagact ggagttaggt caccatcaag aatagcttgt
     2821 tctgaaaagt cattacctgc ttcttgaaga agttcaatat cttctttgac ttgttcataa
     2881 aagggattat tggcaaacag ctgatcaccg tcttcaatac ttgcaaaacg ctcgtctccc
     2941 ttatagagtt ctaaacgttt attatgtaaa tcatataagc cctcaaaggc gcgtcccata
     3001 ccaatcggcc agttcatcgg ataagaagca atccctaaaa cttcttctaa ttcttccaag
     3061 agttctaaag gctcacgccc atcacggtct aacttgttaa taaaggtgaa gacaggaata
     3121 ttacgatgtt tgacaacttc aaaaagtttt ttcgtttgcg cctcaatacc tttagccgaa
     3181 tctacaacca tgacagccgc atccacagcc attaaggtac ggtatgtatc ttccgagaaa
     3241 tcctcatgtc caggtgtatc caaaatatta acacgtttac ccgcgtaatc aaattgcata
     3301 acagatgaag taacagagat accacgttgc ttttcaatat ccatccagtc ggactttgca
     3361 aacgtacctg attttttccc ttttactgtc cctgcttctc taatttcacc accaaaatat
     3421 aatagttgct cagtaatagt cgtcttacca gcatccgggt gagaaatgat ggcaaaagta
     3481 cggcgttttt taatttcttc tgtaagtgac attctttttt ccttgtatag taaaatggca
     3541 gagcttttct ctgagttatt ttcgttaggt attacttgtt tatcaaaaga gttgggctac
     3601 attgggtttc tccatatacg attagcctct aaagttcttc aaaactggta ttataactta
     3661 tcttgggcta tcaatgagtg tcttttttaa aaaaatcgag cctggaaatc attccagact
     3721 cttcttatta tagctgattt taagtgcttt ttcaacgacc tacttttctt tagagaaagc
     3781 aagtgccagc tgttgagctt caccaaattt aaggtaaaaa tattcggtta ttttaaccat
     3841 aaaagtcaag ccacttgtaa cgatcacaaa attaagtagc aaatgacgcg tatgaatcac
     3901 tggaatatcc atcacaaaac cataatttcc tttcgtggct aaactgacca acagaagaag
     3961 aaagttaaca gttgctaaat agcgtactac tgatagcagt cttaactgac tggcatcgta
     4021 aaaacgcaag aggtaaatca aaccattcac taacaaagca taatgaccaa gataaaacga
     4081 gacatttgct acatgccata atctaaacgg ataaagatct ggcgataaaa gtgcaaggaa
     4141 tgttccacct atccccaaaa ccataaataa ttgcttaaac ttattacgat caggtagaaa
     4201 aaagatagct aacatagcga tgcgacaatg atacaacggc aaagcttcat ctaaaggaaa
     4261 cccccttaac acgtaccatg tgtacaagcc aatcacctga gataactgta aaaacaagaa
     4321 aaaataacga taagatttta acctatatgc ttgaaaagtc aaaaaaacaa gcaaaagcgc
     4381 tatcaagagt gacgacaagt aaaagataag tgaggtatga ggcagtccga tggggtcaat
     4441 tgcaaaaaaa tccataacca tccctttcta cgttttttca ttatacctta atctttatga
     4501 gagaacaagc ccttctctgt gctttttcta aaaagtaagg ctaaggtaag tcttcacttt
     4561 aattttcaac taaatgattg accaatttag ccagccccat tgagttactc ccttttaaaa
     4621 gaatttggtc agagggagca agtaattgtt gcacctgtct taccatgtct tcaaactgat
     4681 cttcttgctg atttttttgg aagaactgaa catgtccttc aggataaaac tcatgtgcta
     4741 attgggctaa ctcttgaata tcatcaccat aaaaaacaag ttggtcaatg cgatcaggtg
     4801 acaattgttt gattaactgc gaatgtaggg caacagaatc tggtcctaat tctttcatgt
     4861 cagctagcac tgcaatcttt tttccaccgg ggttctttgc tatgttagca aaggtttcta
     4921 aaatcaggcg catggctgtc ggattggcat tgtaaacatc tgataagatg tcagcaccat
     4981 tagcagcttt tttccattct gtacgattag cagtgagttg aacagactct aacgcctcga
     5041 taatatcctc atctgctact gccaacaatt tgccaacata ggctgctacc atagcattgg
     5101 tcgcgttgta ttttcctggt agaggtaggg acacaggtgt tgctaacaca tttgttgtaa
     5161 acgttaagct atctttttct tctcgaatat cagtaacatt caattcttgg tggtcgccaa
     5221 atcgaatgac catctggttt tctggaagat aaggatcgat aatcgaatca cctggggcaa
     5281 ttaaaatgcc atttgaatcc attccatcaa caatttgcat tttcccttga gcaatcttgt
     5341 cacgtgaccc aaaatattct aaatgggctt cgcctaccag agttaaaaca gcaatacggg
     5401 gtcttgcaat ttcagagaga agatgaatat ctcccatgtg gtcttgtccc atctcaagaa
     5461 caattttttc ggtatcttca ggcatgtgga gaacggtgta gggtaatccg atctcattat
     5521 tgtaatttcc ctgcgtttta taagttttat aggttgttga taggactgcg ctgatcatat
     5581 ctttggtgga agttttacca tttgagcctg tcacagcaat aacatccacg cgcatcttat
     5641 caatataata atgggctagc ttttgaaaag cttttaggca gtcttcaaca agaaggtgaa
     5701 gtttccccgg caattctttt tctgaaaaag tagcgacagc tccattttga aaggctaaat
     5761 caataaactc atgaccatcc ctttgacctt ttaagggtaa aaagaggtct ccttttgtaa
     5821 tctttcggct atcaaattca atgtgatgca gaggaacgtc gtctaagtca gacacattat
     5881 tttgggcgtc aactatttta gccacttcgt gtaaggttaa tttcattttg ttttctttct
     5941 aagaacacta gtatttacga tgtcctatct attatagcac aaaacattag aaaaatttct
     6001 aaaatgtcaa actcccttaa tggctctact attcttccta aaagagcaac aaaaaaagga
     6061 cgaacgtcac atcttgacga tgtttgccgt tccatccaag tcaatgttta ttcatctcat
     6121 aggtttaaat cagatggctc tcacgctgat caaatatttc ttgtgccagt gtcaccagct
     6181 cttcaatcaa atctggataa gccaatccca tattttccca taagagtgga tacatagacc
     6241 attgggtaaa gcctggcatg gtgtttaatt cgttaaggta aacctgtccg tcttgggtca
     6301 agaaaaagtc acaacgagac aacccacaac cacctaaggc tttaaaggct acttcagcat
     6361 aagaacgcat ttctgtcaca atactctggt ctacatctgc tggaatggcc atcgtaatct
     6421 tattgtcaac atattttgct tggtagtcgt aaaaatcaac gtccttgatt acttctcctg
     6481 gtagagttga tttaacctta tcatttccaa ggagaccaac ttcaatttct ctggcaacta
     6541 cgccttgttc aatcaaaaca cgactgtcat aggtcagggc taactgaatc gcttttctta
     6601 actctacttt tgtttgggct ttggaaatcc ccacagaaga acccatatta gctggtttta
     6661 cgaagatagg aaaagtaagt cttgctaaag tttcaactaa acaagcctcc aaatcttgtc
     6721 cgtcaatata aacagtatag gcaacttgtg gaattccaat tgattctaag acacgtttag
     6781 tagtaatttt atccatagct atgctagaag acatgacatt agtccctata tatggcattc
     6841 taagcacttc taagaaacct tggatagagc catcttctcc cataggtcca tgtaatacag
     6901 gaaatacaac tgcaccttct tcatagatat cacttggttt tattttttga gttagctcta
     6961 tcgtctcatt tgtcatcaag cgttctgatt ccgatggctt ttctgaaaat tgctgagttc
     7021 tgataaattg gcccatctga gtaataaaat aagtttttac taagaactta tcatagttga
     7081 ctgctcgcat cacactctct gctgaaagca ctgagacttc acgttctgct gagcggcctc
     7141 catataatag aactaacgtt tgtttggaca tagctaacct ttcttatctg aattgaatgt
     7201 ttttttatta taacaaaaag atgtagcctt ttcaaaacct ctctttgcga gtaaaggcac
     7261 caacatgatc attaaagttc agtacgattc tcaatagctc tcaataatgt tacttcatca
     7321 gcatactcaa tatctgaacc tactgcgaga cctcttgcca aacgagttac cttaatcccg
     7381 gctggtttca agacacgtga aatatacatg gacgttgctt ccccatctgc tgtggcattg
     7441 gtagctacga taacttcgct caccttacca tccattagac gggtaattaa acttttaagg
     7501 ttgatgtcat ctggccccac accattcata ggcgaaatca agccgtgaag cacatgataa
     7561 tagccacgat actcttggat tttttccatg gcagaaacat ctttagcatc ttctactacc
     7621 agaatggtcg tctgatcacg gctcgtgtct gtgcaaatgt gacaaggatc gtcatcggta
     7681 aggtttccac aaatcgaaca ataggtcagt tctcttttag ctgctaataa atttttagca
     7741 aaatcattga catcttcatt tgacattcca atagtataaa aggctaatct cgtcgccgtc
     7801 ttgataccaa ttcctggaag tttagagtaa ctgtcaatta actttgcaat gggtgttgga
     7861 taaagcacta agttctcctt tattgttttc catatatcgc cacataaagc gccctcacat
     7921 ctttgccgat atattcgtaa tatgtacagc aaccgcaata ttatcttcgt tatttgtatt
     7981 ataagctact acatttaagt tgtaagttac taaggtctga tctgaattgt ctgtgataca
     8041 agtctcagca gttccaattt tagcgtgaat cgtgacagaa ccgtttggat tagccaagta
     8101 aaaacttttt tactcgactt gttaccttgg ccttgggata tttggctttt gttttttgat
     8161 tcatgttact tttttagaaa ccgcaaacca tccttatgat ataagtttat aggaattgtt
     8221 tcattccatg cagaaatgat gtttactcaa ttgactatgc tatttttctc aacaatagtg
     8281 cataaagcga actcatggtt atctttaagc tttcatgcta cactttcttc actaaaagtg
     8341 tattaccaaa agcattatta agaaatctct aagcacaaac cataagacag tttgtacgcc
     8401 tgatgaatct gttgctgcat cactgtccac tttttaccct atcaaggcta tttttgaaag
     8461 acaactaatg atctacatta ttttgggaac tactcccata gcaaattctt tttacggatt
     8521 ttttgggaat actcattttt tagggaataa actaaccatt atttagctaa attttttaaa
     8581 cttcgcttct tattttacca taaattataa aaagaagact gagaatagga cgccctcagt
     8641 ctaaaaaaat attttctcat tgagttttac tcttttgcgg atgatagtaa actttaaaat
     8701 ataaaaaact aacaaaaatg gctccaccga taatattacc actatagaca aaaataaaat
     8761 tagtgacaaa atccaaccat gttgctcctc cttcaaaaat agcagcagga atgacaaaag
     8821 catttgctac actatgctga aaaccaaggg ctacaaaggt catgacagga aaccaagtac
     8881 caaggaattt accagcagca tcatttgccc catagcatag ccacaaggcc aaaccaacaa
     8941 accagttaca accaattcct gatactagtg cctgcaaagg gctcgctgct attttagcat
     9001 gggccacctc aatcacttct tctttaaaaa taccagctga tgtcaagcct agaaaatgac
     9061 caaacacaaa agctacaaaa acagctccaa tcacattaaa caaggtaatg ataagccaat
     9121 tcttggcaag ctcgctaaac ttaatttttt tggccaacaa agctgcagaa acagccatca
     9181 tgttccctgt aattaattct ccacccgcca ttaaaataat aattagacca atcggaaaag
     9241 cacaagctcc tacgatactg gaaaaagcac caaaagtttc taaaccactg gcggcaattc
     9301 tcacgtagag taaataacca aggctaatca tcgctccacc aataaagcct aagatagatt
     9361 tagccaaaaa agttttggta accttatgtt ccccaatatg aatagttgct tctaaaatct
     9421 gttctggtgt tttcatgata tctccttttg ttactgtatt cacaaacaca cactttagta
     9481 tatcaatttt cctaaaaaat gcaagccctt tcaacaagaa agggctcgtc attatcatat
     9541 aactatctct attttagtct taggcattta tggtgggaag ttttaagttt tttgctaaaa
     9601 aacgccatag aactaaagca agatagaaat ccaatatgcc atgaataagc gaacctaagc
     9661 caatcaagcc tagcatagac caaaaataag acatagaagt tgctgtggtt gctgttaaaa
     9721 tatagacaac taaaaattca gccaagccat gaatgacatt aatgaaaaac gcaaaactaa
     9781 aacattttac tgccgatgtc atcagttgtg gttttctact taaccaccac gctgccaaaa
     9841 tagcaaacaa taaatgagag agtgctctta aaacaataat taaaggaagt ccagctaata
     9901 aaaaacctaa tgtggtacca agagcgacca aaattgctac tggaatcgac ataaaaatag
     9961 ccaaaaacaa aggaacatgg ctcgccagag taaaggaagc tggtccaata ataagcttaa
    10021 gaggcatgat tatcggaatc aaaatcgcaa atgcgaccaa aatagaggta taagctatta
    10081 gctgtgttgg ttttttaatc attttatctc cttttcaaaa atagtatacc ttaaaaacca
    10141 ataactatca agacacctgt atcttttggt atttttaaac gataccttgt tgctgataaa
    10201 taatcaatag aatattttag catggtattc ataacaacca tcaatggcat aatatactat
    10261 agttatattc ctttgtataa gatacctgct aattctaaat catgcttgat agctaaaaag
    10321 gcttcttgac taggacacga aattaagtga ctatgtagtc cttctgtcaa cgaagacaat
    10381 aactctgctt tagattgact gagtttggac atgaaattag ttacatcaga gtgtgtctta
    10441 atatttaaag gggctgttat cataccgtaa ataggatgtt ctacctctac cgtcgtaata
    10501 atcccttgat gagctaaaat aatttctaat tcttttttag tttcttctac attatgttgg
    10561 cacacaatac gtgcacaaaa ttggtgacta taaagcgctg ttgacataat ataacctttt
    10621 ggagtcgaaa taatatcaat ctgctgagct cgaagaagag ctatatctcc aacgattact
    10681 tggcgactca ctcctaataa cttacctaaa cgagtagcag atacagcttt ttgttcacta
    10741 tttaagcact caataatttt ttgacgacga tcttctgctt tcataatcta tctatccttt
    10801 tttatttcta tcatatcata tttttaagca gggttttctt tcttcttagg atatagaatc
    10861 aaaaaaccag accaaagtct ggtttttaag ttattcacca ccaaggtagt attctttaac
    10921 aatgttaagt ttttcatcta attcaaaaac aagtggtggg aagtttggaa tttccacatc
    10981 catgatttcg tcatctgaaa gacctttaat gtgtttcaca agagcgcgga ttgagttacc
    11041 atgtgcgcca acaaagacgt ttttaccgtc aagaagagct ggagcaattt tttcttccca
    11101 gtaaggcatt gcgcgttcaa gagttacttt taagttttca gcatctggaa taagggctgg
    11161 gtcaagatca gcataacgac ggtctttgtg tgctgaatac tcatcatctt tagccatagc
    11221 tggcggcaat acgtcgtaag aacgacgcca gatatgaact tgttcatcac caaattgttc
    11281 tgcagcttca gctttgtttt tgccagtcaa agcaccatag tgacgctcgt tcaagcgcca
    11341 tgatttttca gttggaaccc acaattgacc tgcattttca agggcaaggt tagttgtttt
    11401 gatagcacgt gtcaatactg aagtgaaagc aaggtcaaat tcaatacctg cttctttgat
    11461 caatttacct gcatcaatcg cttgttgtgt ccctttttct gaaagatcaa catctgccca
    11521 accagtgaag aggttagctt tgttccattc tgactcaccg tggcgagcga aaaccaattt
    11581 taccatgagt taatatctcc ttttaattta aaggttgcta cctttttaca attcctattt
    11641 tacaaaaaat atagcaaaaa agctagtcta ttctgacttt attgaaaatc ataatgtgac
    11701 tatcatgaaa atttcaaatg ttcgtcaata acagtaatat atttacattt ttgtaaaaat
    11761 agcttgctat tattcatgcc tagtggtaga atagaaaaaa tttgtctaaa atatggagga
    11821 aataatggtt tctactgcta cacaaattag tcatttctct tttgataact gtttgatgaa
    11881 tgctgctggt gtttactgca tgacaaaaga agagcttatg gaggttgaaa agtctcaagc
    11941 cgcttccttt gtcaccaaaa cagggacgct taaagttcgt ccaggtaatc cagaacctcg
    12001 ctacgcagat actcgtcttg gttccatcaa ttcaatggga ctccctaata atggttttcg
    12061 ctactactta gactttgtca gtgatcttgc caaaaccggg caacataaac ctcattttct
    12121 ctccgttgtc gggctatctc ctactgaaac cgaaaccatt ttaaaagcca tcatggcttc
    12181 agattatgag ggtctagtgg aactaaacct ttcttgcccg aacgttccag gaaaaccaca
    12241 gattgcttat gattttgaaa ccactgatca acttctagag aacatcttta cctattacac
    12301 taaacctctt ggtatcaaat tacctcctta ttttgacatt gtgcattttg atcaagcggc
    12361 tgcgattttc aataaatacc ccctttcctt tgttaactgt gtcaattcta tcggaaatgg
    12421 gctggtcatt gaggatgagc aagtcctcat caagcctaaa aatggctttg gtggtatagg
    12481 tggagattac atcaaaccta ctgctctagc caatgtccat gcgttttaca aacgcttaaa
    12541 accttctatc cacattattg gtacaggggg agttaaaaca ggacgcgatg cctttgaaca
    12601 tattctttgt ggcgccagca tggtccaaat tgggacagcc cttcatcaag aagggccagc
    12661 tatctttgaa cgggtcacta aggaactaaa aacgattatg gtagaaaaag gctaccaaag
    12721 cttagacgat tttcgtggga acttacgtta caaagattaa ccttaatagt caaaaaagct
    12781 tggaaatgtg cactttgtac actttccaag ctttttaaaa tgggaatttt tttgaaggga
    12841 cagcgtcact ttctttggac tgtctttgct ggttgtttga ttcaactttt cttttttgct
    12901 tgcgctcaaa ttgctgataa aaatagaaaa atagaatgat tttaattatt gaaaatacag
    12961 ctaaactaat gatcataata gtaaacatac ggcggttttc tctttcataa gtcttagtat
    13021 tattataaac actttcaagt tttcaagcaa gtttctaaat tatttaaaag aaagtaaacg
    13081 caagccattt aaaatcacta aaatcgttga gccttcgtgt ccaacaacac caagcggcaa
    13141 gttaactacc tgaaaaacat tggctaaaat caataaggta atcacagata aggcaaaaac
    13201 aatattttgt ttgataatgg tcttcatttt gcgggaaagt tgaatcgaaa atggaatacg
    13261 agtcaagtca tccataatca cactgtctgc actttccatt gcaatatctg ttcctgatcc
    13321 aatagcataa gaaacatctg cttgagcaag ggcaggagca tcattaatac catctcctac
    13381 catggccaca aaaccatatt tggtctttaa ttctgctaac ttagccacct tatcttgagg
    13441 catacagttg gctaccactt catcaatacc aagtttttgt gccacataat tggcagttcg
    13501 ttcttggtcc cctgttaaca tgaccgtctt gattcccata gcatgaagag cttcaatggc
    13561 acgttttgac tctaatttga tgtcatctaa caaagcgtag taggctacta gttggttgtc
    13621 gcgagaaaca aagataagag ttttcccttg actttcttct acctgaatcg tttcttcaaa
    13681 agctgaaaga tctggcacca tctctaaaat aaaggatttc ttcccaatgc gccattcttg
    13741 cccttgatag aagccctgaa aacctttccc agaaatttct tctaagtggt caaaggtcag
    13801 tggctccaat ttttcagtgt attcaagaaa ggctttagaa ataggatggg tactagcagc
    13861 ctctgctcct tttaccagtc tattcacgag caactcatct tccaaataat gagcattcac
    13921 aacagaaggt tttccttggg tgagcgttcc cgttttatcc atgacaacag ccttaatatc
    13981 tcccatgtta tcgacaatat cccctccttt gataatcaat cccttcctgg ctgcacgaga
    14041 aatagcagcc aagctagcag gtgtggaact ggcaattagg gcacatggtg aagctactgt
    14101 taagagaatc atccctcggt aaaaagcagc caaccaagtc caagaaagca caaagtggct
    14161 aaagaggata aaggcaggaa taaggacgag cacaaatttg acataaccat cttctaagct
    14221 ttcgataaag gtggcggttt tgcttttctt ttcttgggca gattctacca gattaataat
    14281 cttggcaaag agagtatcgt cattttcaat agtaaccaac atatctatgg tttgtccttg
    14341 gttaatggtt cccccaatca gatcctggcc ttctgcctta tcaacagtga taggctcacc
    14401 agtgaccata gattcatcaa attgaccaaa aggactaagc aactgcccgt caattggaac
    14461 agcttcgcct ttacgaactt gtaaacgatc accaacactc aaggatctgg tctcaacttc
    14521 taaaatatgg ccatcttctt ggtattgacg agctgtatct ggcgttaagg acatcaaggc
    14581 tgaaatagca tccttacttt tttccatggc catttcttca agcgtattgg acaacgaaaa
    14641 gataaaaata agcagagcac cctccagcca atagccgata attccagcac cgatagctgc
    14701 caaaatcatc aaaatatcca ctgacaagtg tttgttcttc accaaatcca atatacccgt
    14761 ttttgcagac gcatatcctc caatcaagaa ggctgtaatg aaaatagcgg aagccagttg
    14821 tggaaatgag tgtaaaaagg ctaggccaat tattattaat accaaacacg ctaaagtctc
    14881 cattaaatga agatggtctg ccatccattg tctgattgtc atttttagtt ccctcgcaat
    14941 taaaagttat ctcaatcctt aagatatatt aattataata attctaaata aataagtcaa
    15001 gtaatttaga aacattctaa ataaactttt ttaaacttac cttttgcttt caaaaatcct
    15061 aacttcaact atttgcaaat atgaaaaaaa ctccccctac ctgcggaaat gatatactaa
    15121 tagaaggatg actgaccatt atagagtgca tgcattagaa cttagcttaa ctgaagagaa
    15181 aactactaac tttatgcccc aaatataaaa aaagaacctt gtcgctatca ataaacatcg
    15241 atagcaacaa gatttttcta gacgaattat ttaacagcgt cttttaaaga ttacttaata
    15301 ttgtgcacat ataatataat aaactttgat tttataaagc tacaaaataa cgaaacttta
    15361 tataattgct tcgaatcaac tatgtaagtt ttaaacttat gccccctatt taccccttta
    15421 ataaaactac ttctcagggg gcaaagaaaa tacttatctc gaaagctcca ccagcacctc
    15481 ttccactttt tcgtctgtca caatttctcc attctttatc ttctcattcg gcaacacata
    15541 atcaaaaatc tgtccgttct tgcgcacgat cattactgtg tctgctgtga tatatccatt
    15601 gtcaattgct tgtttaaatt cgtcgtatgt tagcataaag cacctcctta tcttattatt
    15661 cgtattttac gagaatatag tacacttaaa tttaaaactt attaaaattt aataacttct
    15721 attttattaa acaaaaagaa attttagcgt aatattattg ataaattaat tttattaatt
    15781 ttaagttttt ttaatttaaa gtgatataat taactagaaa agtgcaatat ttgatccact
    15841 ctaaaagcta aaaatggaaa ggtttaggtg ttatcatgaa attatctaaa caaaaggcaa
    15901 gtttgcttac cgctgtttta ctgttacttt ctctttctat aacgacaata actgttgacg
    15961 cagctaaggt acgaacttat ccgaatgtat cccatgcaaa cacacattat aaaaatactg
    16021 cttctagtaa gctcttacct tttactgcta attatcagtt acaacttggt gagttggata
    16081 accttaaccg cgctaccttt tcacatatcc aactgcaaga tagacacgaa actaaagatg
    16141 tacgcactaa aataaattat gaccctgtgg gatggcataa ctatcaattt ccatacggag
    16201 atggctcaaa atcttcatgg gtaatgaatc gtgggcatct agtcggatat caattttgtg
    16261 gattaaacga tgaaccaaga aatttagtga caatgacagc ctggctaaac acaggtgctt
    16321 attcaggagc aaatgacagc aatcctgaag ggatgttata ctatgaaaac cgtttagatt
    16381 cttggcttgc acttcaccct gacttttggc tagattataa agtcactcct atatatagtg
    16441 gcaatgaagt agtgcctcga caaattgaat tacagtatgt tggaattgat tcatctggtg
    16501 agttgcttac tataaggcta aatagtaata aagaaagtat tgatgaaaat ggtgttacta
    16561 cagtaatctt agaaaactct gctccaaata tcaaccttga ctatttaaat ggaact
//
LOCUS       DAUCWO010000035        16119 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUCWO010000035 DAUCWO010000000
VERSION     DAUCWO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16119
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.035"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(53..124)
                     /locus_tag="VGY20_001104"
     tRNA            complement(53..124)
                     /locus_tag="VGY20_001104"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Glu,seq:ttc)
     gene            complement(103..1195)
                     /locus_tag="VGY20_001105"
                     /pseudo
     CDS             complement(103..1195)
                     /locus_tag="VGY20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1381..1454
                     /locus_tag="VGY20_001106"
     tRNA            1381..1454
                     /locus_tag="VGY20_001106"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1415..1417,aa:Arg,seq:tct)
     gene            complement(1611..2090)
                     /gene="rlmH"
                     /locus_tag="VGY20_001107"
     CDS             complement(1611..2090)
                     /gene="rlmH"
                     /locus_tag="VGY20_001107"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ4273962.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            2302..3525
                     /locus_tag="VGY20_001108"
     CDS             2302..3525
                     /locus_tag="VGY20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ4273963.1"
                     /translation="MPSMKHILKSLSILLVGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVRISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKIVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            3584..4390
                     /locus_tag="VGY20_001109"
     CDS             3584..4390
                     /locus_tag="VGY20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ4273964.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKIPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            4593..5948
                     /gene="dnaA"
                     /locus_tag="VGY20_001110"
     CDS             4593..5948
                     /gene="dnaA"
                     /locus_tag="VGY20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ4273965.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            6103..7239
                     /gene="dnaN"
                     /locus_tag="VGY20_001111"
     CDS             6103..7239
                     /gene="dnaN"
                     /locus_tag="VGY20_001111"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ4273966.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            7314..7511
                     /locus_tag="VGY20_001112"
     CDS             7314..7511
                     /locus_tag="VGY20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ4273967.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            7841..8956
                     /gene="ychF"
                     /locus_tag="VGY20_001113"
     CDS             7841..8956
                     /gene="ychF"
                     /locus_tag="VGY20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ4273968.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            9026..9595
                     /locus_tag="VGY20_001114"
     CDS             9026..9595
                     /locus_tag="VGY20_001114"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ4273969.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            9598..13101
                     /gene="mfd"
                     /locus_tag="VGY20_001115"
     CDS             9598..13101
                     /gene="mfd"
                     /locus_tag="VGY20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ4273970.1"
                     /translation="MDILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGENYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKIKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDILVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYIADERQK
                     IDIYKRIREIRSREDYLNLQDELMDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            13263..13535
                     /locus_tag="VGY20_001116"
     CDS             13263..13535
                     /locus_tag="VGY20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEQ4273971.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            13522..13893
                     /locus_tag="VGY20_001117"
     CDS             13522..13893
                     /locus_tag="VGY20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ4273972.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYAR
                     AKYYLSREGEMIYPIPGLLPK"
     gene            14028..15314
                     /locus_tag="VGY20_001118"
     CDS             14028..15314
                     /locus_tag="VGY20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEQ4273973.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQIPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIPDNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            15311..>16119
                     /gene="tilS"
                     /locus_tag="VGY20_001119"
     CDS             15311..>16119
                     /gene="tilS"
                     /locus_tag="VGY20_001119"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ4273974.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLTIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQL"
BASE COUNT         5544 a         2571 c         2888 g         5116 t
ORIGIN      
        1 ccaactgagc tatgccggaa aaagaaaaaa attgccaaaa tgacaattgc gatagtccgt
       61 acgggattcg aacccgtgtt accgccgtga aaaggcggtg tcttaacccc ttgaccaacg
      121 gaccagaagt tttggaggat atctccttaa cactcttact attatagcag attgattttt
      181 tttgtctata ctttttttaa aaaaaatttg atttttttta aaccgcaagt tgtcgccaaa
      241 tttcaagttg aagttagcca gttttaacat ttctactggc gacttatagt tgaagagtcg
      301 tttaggataa tgattgatcc aatgttcaat agcagcgact tctttagggg tcgttgtttt
      361 ggtaccttta gggagacatc tgcgaatcag gcgattatgg ttttcgttag tgcctctctc
      421 ccatgacgca tagggatggg catagtagat gtcacttgct ggaaagacat ctgataagcg
      481 agaaaattct gtcccgttgt ctgccgtgat agagttgacg gtgtagtctt ttagaatccc
      541 cgtaagagct ttattgacag cttgagcaga cttatccggt attaatcgaa tcagctgatg
      601 acgtgttttc ctgtccgtta gggtgagtaa gcattgattt ttagccctcg tcagaatgac
      661 tgtatcaatc tcataatgcc cagcttcaag acgttggtta atgctatctg ggcgttgttc
      721 aatactcttt cctgcgggtt taaaataagg actggcatgt tttctagcct gcttagcctt
      781 tctcggataa agcatagcct ccttaccaag acttaatttc ccatgatgaa tccagtaata
      841 gatggtagat acagggactt gaactccctt agctttaact atcatctcag gggaatagtt
      901 ctgacgcatg tagtgttcaa tagcctccct atcttgctta cgctgtctaa tgggtctaac
      961 agaacgcttg cgattttctt gatagaccaa ctgagcatgt tgagcagaat aaacggcttc
     1021 aaactttcct ttacgaacct gttgcctaac caatcctcgc ttgatttcat tatgaatggt
     1081 ctggggggcc ttacctagta agcgagcaat ttcacgatta gagcttccag cgttgaattt
     1141 gccgacgctc atggattgtc aattgtttgc cttttggtgt ataataatct tgcatctttt
     1201 tgagtttcct tgtgtttgtg gtgaactaca agtatacctc ttagaggtgc ttttttcata
     1261 ccttaaaagt ggctaagttc attttagaat ttttcaacgc gggatataat gttattttct
     1321 agttttttca aaataaatat tgacagtttt agataatttt tgttaaaatg atgtggttaa
     1381 ggtctcatag ctcagctgga tagagcattc gccttctaag cgaacggtcg caggttcgaa
     1441 tcctgctggg atcaaagata aaaaagcact gttttagtgc tttttttgta tttaccttag
     1501 tgtattgaaa agataggatt tagtctgtct ttttttaatg tttgctttta attataccga
     1561 actattatct aataaagtgg taagagttag ttattccagt tcacactctc ttacttatga
     1621 taaggactgc cttgtgtaat cataaaagca cgataaattt gctcagtaag aactagacgc
     1681 attaactgat gaggtaaggt taacaatcca aaactcatta gcatattagc tcttttttta
     1741 ataattgaat ctaacccaag acttccacca ataataaacg tgatagttga atatccctta
     1801 acagtaaccc ctgatattaa ttcactaaat gtctctgaag gaaactgttt tccttcaata
     1861 gctaatgcaa ttacaaaatc acgttcccca atttttttat ggattcgctg agcttcttta
     1921 ctcataatca attgattatc agcaaaactt gctttatctg gtgttctttc atcagttaac
     1981 tcaatcattt cgaattgaca aaaacgggaa agtctttttt gatattctga aataccatct
     2041 ttgaggtatc tttcttttag ttttccaaca caaattagtt ttactttcat agcttaattg
     2101 tatcacaaga acattacttt ttacacaatt tatccacaag ttatttttct tcaactacta
     2161 ataaaaacct ttgtacaaag gtctttatta gtaaaaagaa aagtaatcca cagcctgtgg
     2221 attacttttc ttttttttat ttttaaggta taattaagca aagttctata agctatggac
     2281 aaataattcg gaggacaaaa catgccaagt atgaaacata tcttaaaatc cttaagtatt
     2341 ttactagttg gatttttagg aggattaata gcaattatta catttaataa tctctaccca
     2401 cattctcctt caaaaataaa ttcaggtaag gcaacaacta gtaatatggt ttttaacaac
     2461 acaaccaata ctactaaagc tgtcaaagct gttcaaaatg cagttgtatc agttattaat
     2521 tatcaagata acccttcttc atccctttct aacccttata caaaactctt tggagaaggg
     2581 cgttcaaaag agaataagga tgctgaatta tctattttta gtgaaggatc tggggtcatt
     2641 tatcgaaaag atggcaactc cgcttacgtt gttactaata accatgttat cgacggagct
     2701 aaacggattg aaattcttat ggcagacgga tctaaagttg ttggtgaatt agttggagct
     2761 gatacttatt cggatttagc tgttgttagg atctcttcag ataagataaa aacagtagct
     2821 gaatttgcag attctacaaa actaaatgtt ggagaagttg ctattgctat cggcagccca
     2881 ctaggaacac aatacgctaa ttctgttact caaggaatcg tctctagttt aagtcgtact
     2941 gtaactttaa aaaacgagaa tggtgagact gtctcaacaa atgctattca gacagatgct
     3001 gctattaacc ctggaaactc tggtggacca ctaattaata ttgaggggca agtaatcgga
     3061 attaattcaa gtaaaatttc ttctacccca actggtagca acggtaatag tggtgctgtt
     3121 gaaggaattg gttttgctat cccatctact gatgttatta aaattattaa acaactagaa
     3181 actaatggtg aagttatcag acctgctctt ggaatatcca tggtcaattt aaatgactta
     3241 tccacaaatg ctcttagcca aattaatatt ccaactagtg taactggtgg tattgtagta
     3301 gcagaagtta aggaaggaat gccggcatct ggcaaacttg ctcagtacga tgtgattaca
     3361 gaaattgatg gcaaaatagt taattcaatt agtgatttac aaagtagtct atacggacac
     3421 gatattaatg atactattaa agtaactttt tatagaggaa ctacaaagaa aaaagcagat
     3481 atcaaattaa caaaaactac tcaagatttg actaaaacgc agtaaacaag ctacctaaat
     3541 actattacat tttatcacat aaaacagtcc aaaggactgt tttatgtttc ttattctatc
     3601 caggaactcc cttatgacaa aagaattatt gatagaccta ccaattgaag atattgttac
     3661 aaatccctat cagccaagga tacaatttaa tcaaagagaa ctccaagacc tagcaacttc
     3721 tattaaatca aacggcctca ttcaacctat tattgttcga aaatctgaca tttttggcta
     3781 tgagttagtt gctggagaac gacgacttaa agcttcaaaa atggctggtc taaaaaaaat
     3841 tccagctatc atcaagaaga tctctacact cgagagcatg caacaagcta tagttgaaaa
     3901 tttacaacgt tctaacctta acgctatcga agaagctaaa gcctatcagt tattggttga
     3961 aaaaaaacac atgactcacg atgagattgc taaatatatg ggaaaatcaa gaccttatat
     4021 tagcaatacc ttacgtctgt tacaactccc agcacctatc attaaagcaa ttgaagaagg
     4081 aaaaattagt gcgggacacg cgcgtgctct tttaactttg agtgatgata agcaacaact
     4141 gtacctcact cataaaatac aaaatgaagg cctaagtgtt aggcaaattg agcaactggt
     4201 tacttctact ccaagttcga agctatctaa aaaaactaaa aatatttttg ccacttcttt
     4261 agagaaacaa ttggctaaat cattgggact ctctgtcaat atgaagctga cagcaaacca
     4321 tagtgggtac cttcagatat ctttttccaa tgatgatgaa ttaaacagaa ttatcaacaa
     4381 gctaaagtag cttgttgata ttctgttttt tcttttttag ttttccacat aaaaaatagt
     4441 tgaaaacaat agcggtgtca ccttaaaatg gcttttcccc aggttgtgga gaacccaaat
     4501 taacagtgtt aatttatttt ccacaggttg tggaaaacta gaatagttta tggtagaata
     4561 gttctagaat tatccacaag aaggaaccta gtatgactga aaatgaacaa attttttgga
     4621 acagggtctt ggaattagct cagagtcaat taaaacaggc aacttatgaa ttttttgttc
     4681 atgatgcccg tctattaaag gtcgataagc atattgcaac tatttactta gatcaaatga
     4741 aagaactctt ttgggaaaaa aatcttaaag atgttattct tactgctggt tttgaagttt
     4801 ataacgctca aatttctgtt gactatgttt tcgaagaaga cctaatgatt gagcaaaatc
     4861 agaccaaaat caatcaaaaa cctaagcagc aagccttaaa ttctttgcct actgttactt
     4921 cagatttaaa ctcgaaatat agttttgaaa actttattca aggagatgaa aatcgttggg
     4981 ctgttgctgc ttcaatagca gtagctaata ctcctggaac tacctataat cctttgttta
     5041 tttggggtgg ccctgggctt ggaaaaaccc atttattaaa tgctattggt aattctgtac
     5101 tattagaaaa tccaaatgct cgaattaaat atatcacagc tgaaaacttt attaatgagt
     5161 ttgttatcca tattcgcctt gataccatgg atgaattgaa agaaaaattt cgtaatttag
     5221 atttactcct tattgatgat atccaatctt tagctaaaaa aacgctctct ggaacacaag
     5281 aagagttctt taatactttt aatgcacttc ataataataa caaacaaatt gtcctaacaa
     5341 gcgaccgtac accagatcat ctcaatgatt tagaagatcg attagttact cgttttaaat
     5401 ggggattaac agtcaatatc acacctcctg attttgaaac acgagtggct attttgacaa
     5461 ataaaattca agaatataac tttatttttc ctcaagatac cattgagtat ttggctggtc
     5521 aatttgattc taatgtcaga gatttagaag gtgccttaaa agatattagt ctggttgcta
     5581 atttcaaaca aattgacacg attaccgttg acattgctgc cgaagctatt cgcgccagaa
     5641 agcaagatgg acctaaaatg acagttattc ccatcgaaga aattcaagcg caagttggaa
     5701 aattttacgg tgttaccgtc aaagaaatta aagctactaa acgaacacaa aatattgttt
     5761 tagcaagaca agtagctatg tttttagcac gtgaaatgac agataacagt cttcctaaaa
     5821 ttggaaaaga atttggtggc agagaccatt caacagtact ccatgcctat aataaaatca
     5881 aaaacatgat cagccaggac gaaagcctta ggatcgaaat tgaaaccata aaaaacaaaa
     5941 ttaaataaca tgtggaaaag aatatctttt atgaaatagt tatccacaag ttgtgaacaa
     6001 ccatttagtc ttggattctc tcgtttattt agagttatcc actatataca caagacctac
     6061 tactactact tattattata cttattaaat aaaggagttc tcatgattca attttcaatt
     6121 aatcgcacat tatttattca tgctttaaat gcaactaaac gtgctattag cactaaaaat
     6181 gccattccta ttctttcatc aataaaaatt gaagtcactt ctacaggagt aactttaaca
     6241 gggtctaacg gtcaaatatc aattgaaaac actattcctg taagtaatga aaatgctggt
     6301 ttgctaatta cctctccagg agctatttta ttagaagcta gtttttttat taatattatt
     6361 tcaagtttgc cagatattag tataaatgtt aaagaaattg aacaacacca agttgtttta
     6421 accagtggta aatcagagat taccttaaaa ggaaaagatg ttgaccagta tcctcgtcta
     6481 caagaagtat caacagaaaa tcctttgatt ttaaaaacaa aattattgaa gtctattatt
     6541 gctgaaacag cttttgcagc cagtttacaa gaaagtcgtc ctattttaac aggagttcat
     6601 attgtattaa gcaatcataa agattttaaa gcagtagcga ctgactctca tcgtatgagc
     6661 caacgtttaa tcactttgga caatacttca gcagattttg atgtggttat tccaagtaaa
     6721 tctttgagag aattttcagc agtatttaca gatgatattg agaccgttga ggtatttttc
     6781 tcaccaagcc aaatcttgtt cagaagtgaa cacatttctt tttatacacg cctcttagaa
     6841 ggaaattatc ccgatacaga ccgtttatta atgacagaat ttgagacgga ggttgttttc
     6901 aatacccaat cccttcgcca cgctatggaa cgtgccttct tgatttctaa tgctactcaa
     6961 aatggtactg ttaagcttga gattactcaa aatcatattt cagctcatgt taactcacct
     7021 gaggttggta aggtaaacga ggatttagat attgttagtc agtctggtag tgatttaact
     7081 atcagcttca atccaactta ccttattgag tctttaaaag ctattaaaag tgaaacagta
     7141 aaaattcatt tcttatcacc agttcgacca ttcaccctaa caccaggcga tgaggaagaa
     7201 agttttatcc aattaattac accagtacga acaaactaag taagaaaaag ctccctttta
     7261 ggagtttttt tgttattata aatattaacg ataatataag tggagaaaag ggaatgtatc
     7321 aaattggatc atttgttgaa atgaaaaaac ctcatgcttg tgtaattaaa gaaactggta
     7381 aaaaggctaa tcaatggaag gtactgagag taggagctga tattaaaatt cagtgtacta
     7441 actgtcagca tgtgattatg atgagtcgtt atgattttga gcgaaaatta aaaaaagtac
     7501 tgcaaccatg aaagtccttt taaataaata attctagcta tatttgcaac caatactttc
     7561 ctaaaaaatt gctagtatgc cgttggaaaa ttagctattc taatgttatc gaaagaagaa
     7621 agatggccat tgacaatgac gaaagttgta gcacagttac ttagtttctc ggcaaactat
     7681 atcaaaatga gaataataca ccaaactttg ataatttaaa tgaaggtgaa agagtggcat
     7741 aatgataagc aaatctgaaa ttttcttctt attcataccg ctttttcttc tattttttgg
     7801 tataataatc ttgattgaaa tttgaatgga gattcgctaa atggctttaa cagcaggtat
     7861 tgtaggctta ccaaatgttg gtaaatcaac cttatttaac gcaattacaa aagcaggagc
     7921 agaagctgct aattatcctt ttgcgactat tgatcccaat gttggtatgg tagaggttcc
     7981 agatgagcgt ctgcaaaaat tgacagaatt gattacacct aaaaaaacag ttccgacaac
     8041 ctttgaattt actgatattg caggtatcgt taaaggtgct tctagagggg aaggtctagg
     8101 aaataaattt ttagctaata ttcgtgaagt cgatgctatt gttcatgtgg tacgtgcttt
     8161 tgatgatgaa aatgtcatgc gtgaacaagg tcgtgaggat gctttcgttg atcctatagc
     8221 agatattgac actattaatc ttgaattaat cttagctgat ttagagtcaa tcaataaacg
     8281 ttatgcgcgt gttgaaaaaa tggcacgaac tcaaaaagat aaagaatcag tagcagagtt
     8341 caatgttttg caaaagatta aacctgtttt ggaagatggg aaatcagcta ggacaattga
     8401 gtttacagaa gatgaagcaa aagttgttaa aggtctcttt ttattaacaa ctaaacctgt
     8461 tttatatgta gctaatgtcg atgaagataa agttgctaat ccagatggta ttgattatgt
     8521 caaacaaatt cgtgactttg cagctactga aaatgctgaa gtagttgtta tctcagcgcg
     8581 tgcagaagaa gaaatttcag agcttgacga tgaggataaa gaagaatttt tggaagctat
     8641 cggtcttact gaatcaggcg ttgataaatt aaccagagca gcttatcatc tcttaggcct
     8701 tggaacctat tttacagcag gtgaaaaaga ggttcgtgct tggacgttta agcgtggtat
     8761 aaaagctcca caagctgctg gtattatcca ttcagatttt gaaagaggct ttattcgtgc
     8821 agtaaccatg tcttatgatg atctaatgac ctacggttca gaaaaagccg tcaaagaagc
     8881 tggacgcttg cgtgaagaag gaaaagaata tgttgtccaa gatggggaca tcatggaatt
     8941 cagatttaat gtgtaattat attaaaacaa tatcagaagg ttggaagaac attccagccc
     9001 ttttggcatt ttagaaagag aaaatatggt aaaaatgatt gttggtctgg gaaatccagg
     9061 ctctaaatat gaaaaaacaa agcacaatat tggttttatg gctattgaca atattgtcaa
     9121 gaaccttgac gttaccttta cagatgataa aaattttaaa gcacagatag gaagtacttt
     9181 tattaatcat gaaaaagttt actttgtgaa acctactact tttatgaata atagcggcat
     9241 agcagtaaaa gcattactaa cctactataa tattgacata acagatttaa ttgttatcta
     9301 tgatgattta gacatggaag tcagtaaatt acgtttacgt agtaagggtt cagcaggagg
     9361 acataatggc attaagtcaa tcattgccca cattggaact caggaattta accgaatcaa
     9421 agttggtatt ggacgacctt taaaaggtat gactgttatt aaccatgtga tgggccaatt
     9481 caatactgaa gataatattg ctatttcgtt aactcttgac agagttgtca atgctgtcaa
     9541 gttttattta caagaaaatg attttgaaaa aacaatgcag aaatttaatg gataatcatg
     9601 gatattttag aattatttag tcagaataag aaagtccaat cctggcactc tggattaacc
     9661 accttaggaa gacaactggt aatggggtta tcgggttcaa gtaaagcatt ggctatagct
     9721 tccgcttatt tagatgatca aaaaaaaata gttgtggtta catcaactca aaatgaggtt
     9781 gaaaaattag ccagcgattt atctagttta cttgatgaag aacttgtttt ccaatttttt
     9841 gcagacgatg tggctgcagc ggaatttatc tttgcgtcaa tggataaagc tctatcaaga
     9901 atagaaaccc tgcaattttt aaggaatcct aaatctcagg gcgttttaat tgttagttta
     9961 tcaggcttaa gaactttatt gccaaaccca gatgttttta caaagagtca gattcaacta
    10021 acagttggag aaaattatga tagtgatact cttactaaac aactgatgac aattggatat
    10081 cagaaggtct cacaggttat tagtccggga gaatttagcc gtcgagggga tattttagat
    10141 atctatgaga ttacacaaga attgccttat cgattggaat tttttggtga tgatattgat
    10201 agtattaggc aatttcatcc agaaactcaa aaatcttttg aacaactaga aggtattttt
    10261 attaatccag caagtgatct tatttttgag gctagtgatt ttcaacgtgg cattgagcaa
    10321 ttagaaaagg ctctacaaac agcacaagat gataaaaaat cttatttaga agatgtatta
    10381 gctgtttcaa aaaacggttt taaacataag gatatccgta aatttcaatc attattttat
    10441 gaaaaagagt ggtcattatt agattatatt cctaagggaa cgccaatctt ttttgatgat
    10501 tttcaaaaac tagttgataa aaatgcaaga tttgatttag agattgctaa tctcttgaca
    10561 gaagatttac agcaaggaaa ggctctttcc aatcttaact actttacaga taattatcga
    10621 gagcttaggc actataagcc agcgaccttc ttttcaaatt ttcataaggg acttggaaat
    10681 atcaaatttg atcagatgca tcagctaact cagtatgcca tgcaggaatt ttttaatcaa
    10741 tttcctttgt tgattgatga gattaaacgg tatcaaaaaa atcaaacaac ggttattgta
    10801 caggtagagt ctcagtatgc ttacgaacga cttgaaaaat cttttcaaga ttaccaattt
    10861 cgccttcctt tagtgagtgc taatcaaatt gtttcacgtg aatcacaaat tgtaattgga
    10921 gctatctcca gtggttttta ttttgctgat gaaaagttag cgttaatcac agagcgtgaa
    10981 atttatcata aaaagatcaa acgacgcgct agacgatcta atattagcaa tgctgagcgt
    11041 ttgaaagatt acaatgagtt agcagtaggt gattacgtgg ttcataatgt ccatggtatc
    11101 ggtcgctttc ttggaattga aacgattcag attcagggaa tccatcgaga ttatgttact
    11161 attcaatatc aaaattcaga ccgtatttct cttccaattg accaaattgg tagcttatca
    11221 aagtacgttt ctgctgatgg aaaagaacct aaaattaata aactcaatga tggtcgtttt
    11281 caaaagataa agcaaaaggt cgctagacaa gtagaggata ttgctgatga ccttctaaaa
    11341 ttatatgctg aaagaagtca gcaaaaagga ttttcatttt caccagatga tgacttgcag
    11401 cgcgcttttg atgatgattt tgcttttgta gaaacagaag atcaacttag gtctataaag
    11461 gaaattaaag ctgatatgga gagcatgcag cctatggatc gtcttttagt aggcgatgta
    11521 ggatttggta agacagaagt agctatgagg gcagccttta aagcggtgaa tgatcacaaa
    11581 caagtagctg tcttagttcc aaccacagtc ttggcccagc agcattatga aaacttcaaa
    11641 gcacgctttg aaaattaccc tgttgaggtt gacgtcttaa gtcgtttccg tagtaaaaaa
    11701 gagcaagctg aaacactaga acgtgtacga aaaggtcaaa ttgacattat tatcggaacc
    11761 catcgactcc tatcaaaaga tgtggtcttt tctgatttag gattgattgt gattgatgag
    11821 gaacaacggt ttggtgttaa gcataaagaa actttaaagg aattaaaaac taaggttgac
    11881 gtcttaacct taacagctac tccgattcct agaaccttac acatgtctat gttaggtatc
    11941 cgagatttat cggttattga gaccccacca accaatcgtt atcctgttca aacctatgtt
    12001 ttggaaaata atccaggtct cgttagagaa gctatcattc gtgaaatgga tcgtggagga
    12061 caaatttttt acgtttacaa taaagttgac actattgaaa agaaagttgc agagctacaa
    12121 gaattagtcc cagaagcttc tataggtttt gttcatgggc aaatgagtga aattcaactt
    12181 gaaaatacct tgattgactt tataaatggt gattatgata tccttgtggc tacaacaatc
    12241 atcgaaacag gagttgacat ttctaatgta aacactttgt ttattgagaa tgctgatcat
    12301 atgggattgt caacattgta tcaactgagg gggcgcgtcg gaagaagtaa tcgtattgcc
    12361 tatgcttacc tgatgtatcg tcctgataag gtcctaacag aagtctctga aaaacgttta
    12421 gaggctatta aaggctttac tgaattaggt tcaggcttca agattgctat gcgagatttg
    12481 tctatacgag gagcaggtaa tattttagga gcttctcaaa gtggctttat tgattcggtc
    12541 ggttttgaaa tgtattctca gttattggaa caggctattg ctagcaagca aggaaaaacg
    12601 actgttcgcc aaaaaggtaa tactgaaatc aatcttcaga ttgatgccta tttaccagat
    12661 gattatattg cagatgagcg ccaaaaaatt gacatttaca agcgtattcg agaaattcga
    12721 tcaagagaag attatctcaa tttgcaagat gagcttatgg atcgttttgg agagtatcct
    12781 gatcaagttg cctatttgtt agagatagct ctgctaaaac attatatgga caatgccttt
    12841 gcggaattag ttgaacgcaa aaataatcag gtcattgttc gatttgaagt aacttcttta
    12901 acttatttct tgactcagga ttatttcgaa gccttatcga aaacccatct taaagctaaa
    12961 attagtgaac atcaggggaa aatcgatatc gtctttgacg ttcgccatca aaaagattat
    13021 agaattttag aagaattgat gttatttgga gaaaggctta gtgagataaa aatcagaaaa
    13081 aacaattcag tttttaaata ataatttgaa acaactttta gactgaatag tattaatgac
    13141 aatggaacga actagctctt tatacttttg tcaaaaatct aagatctttg ctgcgaaatt
    13201 aggatctttt ctaccattta aaaaataaaa atgataaaat gaagaggatt gtaggagaaa
    13261 ttatgagact agataaatat ctaaaggtat cgcgccttat taaacgtcgt tcagtagcaa
    13321 aagaagttgc ggataaggga cgaattaaag ttaatgggat acttgctaaa agttcaacga
    13381 atataaaact aaatgatcac attgaaatta gttttggaaa taaattactg acagtaagag
    13441 tcattgaaat aaaagatagt acaaaaaaag aagatgctct taagatgtat gagataatca
    13501 gtgaaacaag gataacatta aatgaagaag cctagtattg ttcaattaaa taaccattat
    13561 attaagaaag agaatctcaa aaaaaaattt gaagaagaag aatctcaaaa aagaaatcgt
    13621 tttatgggat ggatccttgt aagtatgatg tttttattta ttttgccaac ttataatctt
    13681 gtcaaaagtt atgttgattt tgaaaagcaa aatcaacagg tggttaaatt aaaaaaagag
    13741 tataatgaat tgtcaaagag gacaaaaaaa gaaaaacaat tagcagaacg actaaaagat
    13801 gataattttg tcaaaaaata tgctagggca aaatactatt tatcgcgtga aggagaaatg
    13861 atttatccta ttccaggact attaccaaaa tgatgatgga caatattgta aaaaaaatag
    13921 aagcatttct tgctttttct gataaaaaat tagcagagct gcaacaggaa aatcaaaaag
    13981 ttaaagaaga aagtcagtat attcaaaaaa actagaaagg agtcaatatg agaaaattat
    14041 tagcggctat gttaatgact ttttttctga ctcctttacc agtgattagt acagaaaaaa
    14101 aacttatatt ttcaaaaaat gctgtttatc aattgaaaca agatgtcgtt caatcaacac
    14161 aattctataa tcaaataccc tctaatccaa atctttatca agaaacgtgt gcctataaag
    14221 acagtgattt aactctacca gcaggaagat taggtgtaaa tcaaccatta cttattaaat
    14281 cgcttgtgct taacaaagaa tctttaccgg tttttgagtt agctgatggt acctatgttg
    14341 aggctaatcg acaattgatt tatgacgata ttgtacttaa tcaagtagat atagatagct
    14401 atttttggac acaaaagaaa cttaggcttt attcagcccc ttatgtttta ggtacgcaaa
    14461 caattccttc ttctttttca tttgctcaaa aagttcatgc cactcaaatg gcacaaacaa
    14521 accatggaac ttattatctt attgatgata agggctgggc atcacaagaa gatctagttc
    14581 aatttgataa ccgcatgtta aaagtccagg aaatgctctt acaaaaatat aataacccaa
    14641 attattcaat ttttgtaaag caactcaaca cacaaacaag tgctggtatt aatgctgata
    14701 aaaaaatgta tgctgcaagt atctcgaagt tagcaccact ttatattgtt caaaaacaat
    14761 tacaaaaaaa gaaattagca gagaataaaa ctttgactta tactaaagat gttaatcatt
    14821 tttatggaga ctatgatcca ttaggaagtg gtaaaattag taaaatacct gataataaag
    14881 attatcgtgt tgaagaccta ctgaaagctg tagcacaaca atcggataat gtagcaacta
    14941 atattttagg ttattatcta tgtcatcagt atgataaagc tttccgctca gagataaaag
    15001 ctttatcagg tatcgattgg gatatggagc agcgcttatt aacttctcgt tcagctgcaa
    15061 atatgatgga agctatttat catcaaaaag gccaaattat ttcttacctt tcaaataccg
    15121 aatttgatca acaacgtatc acaaaaaata ttactgttcc agttgcacat aaaattggtg
    15181 atgcttatga ttataaacat gacgttgcta ttgtttacgg taatactcca tttattttgt
    15241 ctatttttac aaataaatca acctatgaag atattacggc tattgcagat gacgtttatg
    15301 gtattttaaa atgatgacct atcaagaaat ttttaatgaa ataaaaaata aagcctactt
    15361 taaaaatcat agacatgtgt taatagccgt atctggtgga gtagattcaa tgaatttatt
    15421 gcactttttg tacctgtttc aagacaaatt gacaattcgt attggtattg ctcatgttaa
    15481 tcacaaacaa agatcagaat cagattcaga agaagcttat ttaaagtgtt gggctaaaaa
    15541 acatgacata cctatttatg tttctaattt tgaaggaata ttttcagaaa aagcagcacg
    15601 tgattggcgc tatgcttttt ttaagagtat tatgttaaaa aataactatt cagctttagt
    15661 aacagctcac cattcagatg atcaagcaga aactattttg atgaggctaa ttagaggaag
    15721 ccgattgagg cacttgtcgg gtataaaaag cgtgcaacca tttgccaatg gtcaacttat
    15781 aagacctttt ttaactttct caaaaaagga cttgccagaa atttttcatt ttgaagattc
    15841 ttcaaataga gaattatcat ttttaagaaa tagagttaga aataattatt taccactgtt
    15901 aaagcaggaa aatcctaggt tcattcaggg attaaatcaa ttagcacttg aaaatagctt
    15961 actttttcaa gcttttaagg agctaacaaa tcatattact accactgatt taacagaatt
    16021 taatgagcaa tctaagtcaa ttcaatactt tttgttacaa gattatttag aaggttttcc
    16081 agatttagat ttaaaaaaat ctcaatttac tcaattatt
//
LOCUS       DAUCWO010000036        16044 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUCWO010000036 DAUCWO010000000
VERSION     DAUCWO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16044
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.036"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(195..2186)
                     /gene="uvrB"
                     /locus_tag="VGY20_001120"
     CDS             complement(195..2186)
                     /gene="uvrB"
                     /locus_tag="VGY20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ4273975.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2379..4553
                     /locus_tag="VGY20_001121"
     CDS             2379..4553
                     /locus_tag="VGY20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ4273976.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLFAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4553..5293
                     /locus_tag="VGY20_001122"
     CDS             4553..5293
                     /locus_tag="VGY20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4273977.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5441..5593)
                     /locus_tag="VGY20_001123"
     CDS             complement(5441..5593)
                     /locus_tag="VGY20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273978.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(5590..6969)
                     /locus_tag="VGY20_001124"
     CDS             complement(5590..6969)
                     /locus_tag="VGY20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4273979.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(7147..7596)
                     /locus_tag="VGY20_001125"
     CDS             complement(7147..7596)
                     /locus_tag="VGY20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ4273980.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYQLEALGENRTRITYSYD
                     YHPLDIFQKANQFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(7593..7928)
                     /locus_tag="VGY20_001126"
     CDS             complement(7593..7928)
                     /locus_tag="VGY20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4273981.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(7931..8242)
                     /locus_tag="VGY20_001127"
     CDS             complement(7931..8242)
                     /locus_tag="VGY20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4273982.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8265..10259)
                     /locus_tag="VGY20_001128"
     CDS             complement(8265..10259)
                     /locus_tag="VGY20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ4273983.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVSQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNIFLEGINFAEIMIIVLDECRDAKLKLSDFVI
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLITRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LSKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVSICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(10365..11459)
                     /locus_tag="VGY20_001129"
     CDS             complement(10365..11459)
                     /locus_tag="VGY20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEQ4273984.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAERSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEQIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(11468..12868)
                     /locus_tag="VGY20_001130"
     CDS             complement(11468..12868)
                     /locus_tag="VGY20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4273985.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSNAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            13093..13788
                     /locus_tag="VGY20_001131"
     CDS             13093..13788
                     /locus_tag="VGY20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ4273986.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDSSQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(13793..13951)
                     /locus_tag="VGY20_001132"
     CDS             complement(13793..13951)
                     /locus_tag="VGY20_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4273987.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(14020..15333)
                     /gene="obgE"
                     /locus_tag="VGY20_001133"
     CDS             complement(14020..15333)
                     /gene="obgE"
                     /locus_tag="VGY20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ4273988.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLATQYDEFNDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDEADLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
     gene            complement(15390..15518)
                     /locus_tag="VGY20_001134"
     CDS             complement(15390..15518)
                     /locus_tag="VGY20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ4273989.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
     gene            15871..>16044
                     /locus_tag="VGY20_001135"
     CDS             15871..>16044
                     /locus_tag="VGY20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4273990.1"
                     /translation="MIRLIDRYGVTIVQKCKNNYYSPELKQEIINKVLIDGQSQKQTS
                     LDYALPTSSMLSRW"
BASE COUNT         4865 a         3388 c         2860 g         4931 t
ORIGIN      
        1 ctaaaagtca tattattaaa aagaaaattt ttttaaagct taactattta gacagttaaa
       61 ttttatgcct ttttactaaa cttactaagt taatctttac tttaaaaagt taacttaaaa
      121 ttgtcctaat aaaaagaatt atttgacttt aacttatata tgttaataag tataatataa
      181 gctttattca aaacctagtc cattaattta agttctaaaa tcaagtctcg catttgagct
      241 gccaattcga aatcaagtaa ttcagctgcc tcttgcattt gtttttgcag agcattgatc
      301 gcttcttttc gttctcctct tgacatactt tcataatcca tctcttcttt tgatatatca
      361 ttgtggcttg ttttacttat actaataagg cctctgatgt ctttttttat agtttgtgga
      421 acgatgccgt gcgctttgtt atatgctatt tgaatctcac gacgacgagc cgtttcgtca
      481 atagcccgct gcatagaatc tgtcatcttg tccgcataca taatgacatg accatctacg
      541 tttcgagctg ctctaccgat ggtctgaatc aagccgcgtt cattacggag gaaaccctct
      601 ttatcagcat ccaaaatggc tacaagacta acttctggca catcaatccc ttcacggagc
      661 agattgattc caatcaagac atcaaaaaca ccaagtcgta aatcacgaat aatttctgtc
      721 cgctctaagg ttttaatatc actgtgcata tacttgacct tgacacccat ctcttttaga
      781 taatcagtca aatcttctgc cattttttta gttaaggttg tgatgaacgt ccgttcatcg
      841 cgcgctacgc gctggttaat ttctcccaac aaatcatcca tttgccccat acttggcctt
      901 acatcaattt caggatcaag tagcccagtc ggacgaataa tttgctcaat aattgtatta
      961 gtctgtgaca tttcgtattc accaggcgtt gctgaaacat agacaatttg gtgcacatga
     1021 ctttcaaatt cctcgcgtct taagggacga ttatcaagag ctgatggcaa tcgaaaacca
     1081 tagtcaacta gcatttgttt tctggcttgg tcaccattat acatgccttt gatttgtccc
     1141 attgtcatgt gactttcatc aatcataatt aaaaaatctt ctgggaagaa atctaacaag
     1201 gtataaggag gttctcctgg tgacctgcca tccatatgac gggaatagtt ttcgactccg
     1261 ctagtatagc ccatttcacg taacatttca atatcatatt ccgttctttg gcgtaatcgc
     1321 tgtgcttcta gtagtttacc ttcagattca aataactgta actgctctgc taactctgct
     1381 tgaattttgg ctattgattg ctccatatgc tcatcatttg ttacaaagtg agtagctgga
     1441 aataaaacca aatggtcaac ttctccaata gttttcccag ttaaactttc gatttcacat
     1501 atacgatcaa tctcatcccc gaaaaattcc acccgaaaag catgttcatc cctggatgct
     1561 ggaaagacct caacaacatc gccacgtaca cgaaaacagc cgcgttgaaa atcaatatca
     1621 ttacgctcaa attgaatatc tactaattga tttagcaaag tgtcacgtga tatctcctga
     1681 cctggacgta ggctgactgc tgaatctgcg tactcttttg gcgaacctag tccataaata
     1741 caagatacag aagctaccac tatgacatcg ttacgctcaa gtaaagaaga ggtggctgaa
     1801 tggcgaagtt tatcgatttc atcattgact gaactatctt tttcgatata ggtgtcgctt
     1861 gatgggacat atgcttcagg ttggtagtaa tcataatagg aaacaaagta ttccacagca
     1921 ttgtctggaa aaaattcttt aaattcacca tataattgtc ctgctaaggt tttattgtga
     1981 gctatcacta aggtaggttt atttacttta ctaattactt ggctcatggt gtatgttttt
     2041 cccgttcctg tagcacctag gagaatctga gctttttctc cgccctcgat attatcaact
     2101 aggctttcaa tagcttgtgg ctgatcacca gacggtttat actttgattt tagtttaaat
     2161 ggtttatcat cacgtttatc aatcatttta taccctctaa tagtcttact ctcttataga
     2221 ctattttaac atagataagc cagaatcacc tccaataagc gtaaaaaaca tgtcatttac
     2281 atgttatgtt ttatgacata taaataacaa atttcttgct taatgttcaa tattttgtta
     2341 taattaacca tattttttag ttttataagg agattagtat gacgcacaaa ataaaagtat
     2401 tgctgcttgc gataatgtct atttttttga catgcaatat tgcaagtgct gaaactattg
     2461 ctattgtttc agatacagct tatgccccat ttgaatttaa agactcagat caaatttaca
     2521 aaggaattga cgttgatatt attaatgaag tagccaaacg tcaatcttgg gatttcagta
     2581 tgagtttccc gggttttgat gcagctgtaa atgctgttca atctggtcaa gcgagtgctc
     2641 taatggccgg tacaaccatt acgaatgctc gtaagaaagt ctttcatttc tcagagccat
     2701 attacgatac caaaattgtc attgcgacac gtaaagccaa tgccatcaaa aaatacagtg
     2761 acttaaaagg aaaaacggtc ggtgttaaaa atggaacagc ggctcaagcc tttttgaata
     2821 actataaaaa aaagtatgat tatactgtta aaacatttga cacaggtgat cttatgtata
     2881 atagtttatc tgctggttct attgccgctg ttatggatga tgaggcggtt atccaatacg
     2941 caatcagcca aaaccaagat attgctatta acatgaaagg agagcccatt ggaagctttg
     3001 ggtttgctgt caaaaaggga agcggatatg attatctagt taatgatttc aatacagctc
     3061 ttaaagctat gaaagctgat ggtacctacc aagctatcat gaccaagtgg ttaggcacag
     3121 atgataaagc taccaccagt caggcaacgg gaaatccatc tgccaaagct acacctataa
     3181 aggacagtta taaaattgtc tctgattcgt cttttgcacc ttttgaattt caaaatggta
     3241 agggcaaata cgttggtatt gacatagaat taatcaaagc tattgctaaa caacaaggtt
     3301 tcaaaattga aatcgctaat ccaggtttcg atgctgcctt aaatgctgtg caatctagcc
     3361 aagcagatgg ggtcattgct ggtgcaacta ttactgacgc tcgtaaagct atctttgatt
     3421 tttctgatcc ttattatact tctaatatca ttttagctgt taaagctgga aaaaacatca
     3481 agaactatga agacttagac agaaaaacag tcggtgctaa aaacggtact tcatcttact
     3541 cttggttaaa agaaaacgct cctaaatatg gttataatgt caaggcattt gatgatggtt
     3601 ctagcatgta tgatagctta aattcaggtt ctgtagatgc tatcatggat gatgaggcgg
     3661 ttcttaaata cgctatctct caaggtcgtc gctttgaaac acctcttgag ggcatttcta
     3721 ctggtgaagt tggttttgct gtcaagaaag gaactaatcc agaattaatc gaaatgttca
     3781 acaatggctt agctgctctc aaaaaatctg gtcagtatga tgacattata gataaatacc
     3841 ttgactctaa gaaagctgca actccttctg aaaaaggtgc tgatgagtct actatttcag
     3901 gcctattatc aaataactac aaacaactat ttgcaggact tggaaccacg ctcagtttaa
     3961 cccttatttc atttgctatt gctataatta tcgggatcat ctttgggatg atggccgtgt
     4021 caccaactaa atcacttcga ctcatttcaa cggtctttgt ggacgttgtt cgagggattc
     4081 ctttgatgat tgtggctgcc ttcattttct ggggagtacc aaaccttatc gagagtatga
     4141 ccggccacca gtcaccgatt aatgatttct tagctgctac aattgcactg tcacttaatg
     4201 gcggagccta tattgctgaa attgttcgcg gtggtatcga agctgttcca gcagggcaaa
     4261 tggaagctag tcgaagtctt ggtttgtctt acggaaccac gatgagaaaa gtaattctcc
     4321 cacaagctgt gaaactaatg ttacctaact ttatcaatca gtttgttatt tcattgaagg
     4381 atacaacaat cgtctcagca attggtttag tggaactctt ccaaacaggt aaaatcatta
     4441 ttgctagaaa ttaccagtcg ttccgcatgt atgctatttt agcaattatt taccttatca
     4501 tgattacact cttaacaaga cttgcaaaac gtttagaaaa gaggcttaac taatgacaga
     4561 attaaaaatt gatgttcaag atttacacaa atcttatggt caaaatgaag tcttaaaagg
     4621 aattgatgcc aaattctacg aaggcgatgt cgtttgtatc attggtcctt ctggttctgg
     4681 gaaatcaacc tttctacgta ccctcaacct cctagagacg attaccagtg gtaaggtcat
     4741 ggtggacggt tttgaattat cagatcctaa gactaatatt gacaaggcac gtgaaaatat
     4801 cgggatggta ttccaacact tcaacctttt ccctcacatg actgttcttg agaatattat
     4861 ctttgctcct gttgaattag gtaaagagtc taaagaagtt gctaaaaagc atggcatggc
     4921 acttctagaa aaagtcggac tttctgataa ggccgacgcc ttcccaggaa gtttatccgg
     4981 cgggcaaaaa cagcgtgtcg ccatcgccag aagtttagca atgaacccag atattatgct
     5041 ctttgatgaa ccaacttctg ctcttgaccc cgaaatggtt ggggacgttt tgaacgttat
     5101 gaaagacttg gcagaacaag gcatgaccat gttaattgtt actcacgaaa tgggatttgc
     5161 tcgccaagta gctaaccgtg ttatctttac agacggtggt caattcttgg aagatggtac
     5221 tcctgaagag atttttgatc atcctaagca ccctcgtctc attgaatttt tagacaaggt
     5281 cttaaacgtc taatagagca gacaccatac taacttccaa aaaataataa ggcaaggaaa
     5341 caggtttcct tgcctttttt ggttactgtt attattcaaa ccagcaaaat agggatactc
     5401 ttatgactct ctccttcata agagtgtccc ctatgctttt ttatgggcgt ttatgtttag
     5461 tgaggtaata acctaatccc gtaagagcta gtcccgtcac tatgtgtaag tatttgttac
     5521 caaccatcgg actaatggca atgagaaaaa agccaacagc cattaaaaaa gttgctccat
     5581 ttttttctgt catgcaaggt cactcgcttt ttctttattg gccaaaatga caaatggtgc
     5641 ccagataagg aaggcaacaa agaggttaaa cagtgaaacc aaacctgcca taatattacc
     5701 tccagtagct aggtaagcat aaagtccaac aggggtaatc caaggtactg ataagaatac
     5761 cggtggaatc aaacctattg cagtagcaaa ataagctatg ctagcacaaa ttggtggcac
     5821 aattagccat ggaatcacaa aggttggatt caaaacaatt ggcatcccaa atgtaattgg
     5881 ttcgttgata ttgaaaacac ccattggtgc ggacaatttg gcaatggttt tatgctcttc
     5941 tcgttttgag aatatgaaga tagcaataat caatgccaag gttactcctg aaccacccat
     6001 ttgagcataa gcatcaaagg acccgcgtgt ccaaaggtat ggtaaattag cagcgctatg
     6061 cgctgtattg taagcagctg tattttctgt taaagctacc atataaatac catccatcac
     6121 tggggctaag acattatgac catgcaagcc gaagaaccag agcagttgaa ctaagaaggt
     6181 taacaagata actgaaccaa taccctgtga taagcctagc aatggtaatt ggatataggt
     6241 tgaaatgaca tcacttaggg aagaacctgt taatgcgaaa atgaggtagg cgaaaattgc
     6301 cgaagcgtaa atcgcaactg ttcctggaat aatggcagca aaggctttat taaccgcagg
     6361 tggtacatta tctggtaagg taatggtaat attacgtttg gtcaaagccg catacacaaa
     6421 acttgataaa aagccaataa ttaaagctgt gaacaagcct gtggcaccga catactttag
     6481 agctattgct ccccattgac tagtttcaat acttgaagct ccacccgcag taacgactga
     6541 aagccccatt tctttcaaat tgctaattag gttagcagag gcattttcaa gtggtgtcga
     6601 aatggttaaa gtctgaggta gggtggcaat aaatgagcca aaagaaatca atcctcctgc
     6661 caatggatta atcttatgca taacagctaa attgtaaccg aaagcaaagg caaaaaagag
     6721 cgccatgata ccaattgtcc caaaataaac atagccattg atgtcgataa caggttgcat
     6781 agcttttgca aatcctgtcc atcccatatt gtttggaata tcgcgtaaaa aaacgttcaa
     6841 taagacagca actgaacctg ccatggtaat aggcatcatt gaaataaagg aatcacgaat
     6901 agctactaaa tgccgttgtg acccaatttt tcctgaaatc tccataaact tatcagaaaa
     6961 atgcgacata aatcttctcc tctttttatt tttttaatgt aacaaaggta accttaaaag
     7021 cagcggtgtc tgcctaacac aattatctca aaatcatcct tgttaaacgt gatgcttaga
     7081 gataaccggt aatcggctct tatccaaatg aacccttctc tctcattcaa ggtctgtgaa
     7141 taatcttcac aaagacgatg gttgctgcaa ctgtttagca tattggataa gggctgctaa
     7201 ctgagcttga gattggcgtt ctaggctttt tttgaacagt ttgccaacaa taaattgatt
     7261 tgctttttga aaaatatcta gaggatgata gtcataagaa taggtaattc tagtccgatt
     7321 ctcccccaga gcctctaatt gataagccaa ttcctttatc ccatgattgg aactaaaaca
     7381 agcagtatat aattgcggag cttctagggc cgtaataagc accctcactg tacgttcttt
     7441 gtatttacca taatgtttaa catagtttat ccccgctttg atatcatctc ttgacaactg
     7501 tctcaaggta ttttgtcgat aatcttcttg gagcgatatc agcatggctt caaaacaatg
     7561 atgaatggaa acatctcctt ctgctgaaat aatcatgact cacctctttg tattgctgac
     7621 attttgtcgt aaacggtaac caattccttg gctaaatcaa taaaggctaa ggcagtcatc
     7681 atgtgatctt gcccatgcac catcaataaa gacaattcaa ccgcatcacc agctgcttct
     7741 tgggacagca aatgtgtctg cgatttatgg gcaatattta acgagtttgt cgcttcttga
     7801 agtaagtgct gagcctcctc aaactgccca tctcgtgctg catgaatagc ctctacggcc
     7861 ttgcccttgg cttcgcctcc atacatgatt aagcccataa tagcttctaa gttggatggt
     7921 tccgtcatgc ctactcctcc atcattgcta gggctgtttc aaggactttt tcaccgttca
     7981 taagaccata atcagccatg ttaatggcat ctactttaat atctggttca aacttatctt
     8041 taaaatcctt tagtaagaat ttgacttgtg gacctaacaa gagaacgtca atgctattag
     8101 cagccacttc gttgtcagct tcactcacag gaactgccca aatagaaact tcaaggtcac
     8161 gcgcttgggc tgctttttgc atcttagtca ccaacatact agttgacata ccggcattac
     8221 aaactaacat aatagatttc ataaggtttc tccttttaat cgttttaatc atgaataagt
     8281 ggggtaaaca ctgtcatgaa tttggaataa tttggttctt ctagtaacgc ttgctgaagc
     8341 tccacctgat tgacaaaaga tgccaaacct ggtgatacat attttaacct atgattatgc
     8401 cctttagacg gggataatag aaagacaaaa tgaacagctt gatgcgcctt gtcccactca
     8461 agaggctctc ggcaaataga cactactacc tgttcagaat aagtcatggg attagcagga
     8521 tgcggaaagg caagcacttc tccatagatg actgggctgt aattttccct cagtaccatt
     8581 tgatgataga agtcctctac aaacgtttct gttcccgcct cgtcaagaca agcaatcatc
     8641 cgtaaaagca agtcttcctt tgagattttc tcgtctaaat aaagaaaacg attagggcta
     8701 aaaacacctg ttagaagctg ctctgctttt gataaactca tctgtgacgg taaactgacc
     8761 tctttcttga taccttcttg ttctccaata aattgtcgaa tagtttcaat atcctgattt
     8821 gacaaaaaaa cactgacagt aatcactggt gttaaaaaca tcagattggc aaggctgata
     8881 gaggaaataa tcaaatcaac ggttttcagt cgctcttcgg ttatttcata gtaactgata
     8941 acatcgacaa tacgcaaacg tccttcaaat tccttttcca accgattttt caacatcatg
     9001 gcactaccat aacctgtggc acaaactact agaaccctca atttatgacg gtttgaataa
     9061 cgctcaatgg ccgccattaa atgcaaacta atataagccc attcatcatc tgagacatca
     9121 ttcttttgac atacaagcaa atccgaaaag gtctgcttgg taagggtaaa tatttcagga
     9181 tactggcttt taatttcctc agttaaagga ttagtcagct gaatatggtt ttcaagacgt
     9241 gtgatcaagg gcatcatgtg ggctaataaa ccttgaaata aattggtatc tgtttctaaa
     9301 gtcatgcctg tcaattgact aatcttggca atacatgctt ttaaatggtc ctgcaacttt
     9361 tcatcggtat tatcatcttg ccaatgctta ccaacagaat gtttgacctt tagatgcagt
     9421 gcaatataat tagcctcctc tttaggaaaa cgtatcccca tcacttcttc cactcgatac
     9481 aaaatgcgca gagctacttg atactcatca gactgacgaa tagctattgg aatggaaaac
     9541 agttctaatg gacaccccga tcgaatacgc tgaaccatta aggcaatatg caagactaga
     9601 ttatttatga caaaatcaga aagtttcaac ttagcatccc gacattcatc caagacaata
     9661 atcatgattt ctgcaaaatt aataccttca agaaagatat tacctaccat ggtggacaag
     9721 gtattatcaa aactagtaac aaaaaaatag tccatgataa aatgcctaat atcctcttct
     9781 ttacctctta tttgaatatg ttggtgagtt atttcaagct ctaactggta aggtattatg
     9841 cgctccttaa tgcctgctaa aacatgtttt aaagttgtcc gactgatata caattcttga
     9901 caaagctctt caaaatcctg aacggcatct tcaaaaaaga atttatgaag cacataatct
     9961 tctcgatcaa ccgattcttc cacttgagac acatctgcca aacgtttttt agacgtcaca
    10021 ctttcttgcc aaaagatgtc aaatgccata gaatggtcaa tttctaagca gtaacctcga
    10081 ccttgtttag aaatgatgtg agctccgtgt tcaggaaggc tactgatcag ttctttgagg
    10141 tatttcctaa ccgtacggtc actcatacca ataacctcag ccagagttgc actagagaca
    10201 aactgctctt tgtgatggat tagaaatgtt atgatagcca gttctttttt tgataacatt
    10261 agtcctcctt actactttag aaaacgcttt ctaccctctt ttagtgtacc aaatatttcc
    10321 tgaaaaaggt gaccatcatt ttccgcattg attgtgaaaa gaatctatga tgggtcaatc
    10381 tccaaacaaa attcctgcca aggtcgcaaa tcatctaaca aactaagata gtcctctctg
    10441 acctcagcaa ccacattagc ttttgaactg accgtaaaat ttttcaaagc aatttgcagt
    10501 tctcccttat atcgatgata gcggtcattg tcaataataa tgctacctcg tttgacctcc
    10561 ttagattggt ctcttggagc aatcgattcc tgagcataga ctagacgtgg catggttgat
    10621 cgaatcacat aatcagatac atcgccacga taacaatgcg gatagccaat gatttgctcc
    10681 tcaacttctg tcaaatcgat gataggccta accttgatcg ttgtgactgg ccttgctaaa
    10741 gcctgagtac atgctgccaa ttcttcttcc gaaatgaatt ggttagatat gaggatatta
    10801 tcaatggttc ctatcgcttt cattaattct acttgcaggc caatgggcaa gtgcctatgg
    10861 tcttccactg tcggcaagcc ttctgccagt agccaagggc cttcagatgc tgactgagcg
    10921 gtaataaaag cagccgtttc aatatcatgc tcatgataaa aacgtgacat atccttaaaa
    10981 tgttgccatg acaaaccggt aaattcatgt ggataaaaat tatggcaacc aataatattg
    11041 ctccgttctg catctgtcgc tagcaatgac atcaataatt gtttatcagt gctcatatta
    11101 agttcaattt taagcccaaa gggatttctt gttaaagtta caatctctgc tagagggaga
    11161 gcctcgtcta agcgtaagcc cgctaacccg aaggcatgag ctcgttcaat aagctgatct
    11221 gaccaaccag cttggctaat aaagcttgga ctaacgtcag caatcactcg aatgcctaat
    11281 tgatttgcat aagcaatgag ctctgcgtag caatgaaaca tctggtgatc agctggtgcc
    11341 agctgtaaga gactcataaa caagcgcttg gctccatagc tatgacataa gtcaatataa
    11401 gctttactct tggtaacatc ataccgctca gggtatagag aaaatcctaa atcaaccata
    11461 ttgcctccta tttatcaaaa ccatttgact cgctcaacgc cttaaaccag taacctgatt
    11521 tttttatggt tcgcttttga ctctctaaat ccaaagctac caaaccatat cggtttttat
    11581 aagcatttaa ccaagaccag caatcaataa aagtccacaa gaggtagcct ttgcagttgg
    11641 ctccttctcc caaagcctta tgaagctgga taagatgatc ctctataaag gtgatgcggt
    11701 agtcatcttg tatctggcca tctgctaaaa aagcttcttc accttcgaca cccataccat
    11761 tttccgtgac tagccattcg atgttaccat agtgttcttt taaattaagg gcaatatcat
    11821 aaagtcctgg ttcataaatt tcccaacccc ggtgaggatt gactttttta ccaggcatat
    11881 cataaggttc aaaataaata tctaagataa gggggtcccc ctcttgctgt gacttactag
    11941 gtgcctgaac ccgcaaaggc tgataataat taactcccaa aaaatcaaca gtattgtttt
    12001 tgataatctc taattcatca gcactatatt gaggtaataa atcagcttcc tctaaaatgg
    12061 aaactaactc tgctgggtaa acacccaaaa ctgatggatc taggaaggat ttggtttgga
    12121 aaagctctgc gattctagca gccttgacat cttcaggagc attactccga ggataagcag
    12181 gtgtcatgtt aagcacaata ctaatcttgt ggtcgggatg aagcttatga caagctttga
    12241 ctgccagtga actggccagt tgtgtgttgt aagccacttg aactgctgcc ttggcatcta
    12301 ctttacaagg gtagtgataa taacctaaat agccacattc gactggaaca atcggttcat
    12361 taaaggtaat ccaagtgttt actaaatcac caaaaagctc aaaacaggtt tttgcgtagg
    12421 tttcataagc ccagacagta gctttgtttt cccagccacc tttttcttgc aaggcatagg
    12481 ggaggtcaaa atgatagagg ttaacaatca atttaatacc ttgagcaata atgtcttgaa
    12541 atacttctcg ataaaatgtc accgcctttg gattaacctc cccgactcct tctggaatca
    12601 atcgagacca ctggattgag gtccgaaaaa tcgtgtggcc tgtttctttc aaaagggcaa
    12661 tgtcaccctt atagttttca taaaaggttg aggttttttc tggaccaatt tggtgatgaa
    12721 acttagctga ctcaatacta aaccagtaat cccagttgct aggtcctttg ccatctccag
    12781 gaaccgttcc ttcgctttga ggcccagatg tggaacttcc ccataaaaag ccatctggaa
    12841 attgataacg gtgttttctt tgtttcatat cctttgtcct ttctttgctc acttctcttt
    12901 ttattataag aagaaaaaca ggtgatgtaa acgctttaaa aaatctgttt tttccttatc
    12961 atcacggaaa ataagtgatt taaagcttga catattttat tatcttaaag agcctatcta
    13021 acctgccctg ctacttctta tcaaaaccaa gcccctattg acagatgcct tagcctatgt
    13081 ttgaataggg gtatggctta tttaaactac tttacaaaaa ccgagtgggc actttggtta
    13141 agctctgtct tggccatttt aattagcgcc cttttattcg gtaagcaagc tcccttagct
    13201 ttaatagctt ctttgattgg tgtgacctct ctcattttta gtgctaaagc taatcctatt
    13261 ggtcaagggc tcgtcattat tttttccatt atctatgcct atctttcatt acgaaacagt
    13321 tactatggag agttaatgac ctatcttttc atgaccctcc ctatgactat tttttcactt
    13381 tttacttggt taaaccatcc ttttgaaggg aaaaaatcac aagtgactat ttcacgatta
    13441 acacccactg atcgaagatg tcttttcgtt tttactatac tggtcactct tattttctac
    13501 ggcatcttgg cttctattca aacagcctat ttacttgtct ccactttatc gattgccact
    13561 tctttctcag ccgtttatct cagttataag cgtagccctt attttgcttt agcatacggg
    13621 ctcaatgatc tagtcctcat tctcttatgg atgcatgctg ctcaaacaga ttctagccaa
    13681 tatgcagtag tcatctgctt tgccaccttt cttattaatg atagttacac tttttatagt
    13741 tggctaatgc tccaacgtta ccaagaaaaa aaggtcaaaa aaggatgagg gcctaagcct
    13801 catccttttt caatttttca cgttctaagc gcaaacgtct attgggattt aaaagattaa
    13861 acaattcttc taatttctca gcattccaaa catctgctgc cgatacaaaa tttccatcct
    13921 tatccttaaa ggatatcggt ttatcaccca taatcagaaa cctccatctt aaagataaca
    13981 cacaagagca ctatcacact gctattgtaa tggattgcct taatccacaa attcaaattc
    14041 aaacttgcca atacgaacag gatccccatc ttttgctcca cgttcacgaa gggcttcgtc
    14101 aacccccata ccacgcaatt gacgggcaaa tttcatgatg gactcatcac gttccatatt
    14161 ggtcatgaca aagagacgtt ctaacttctc accagacaag acccaagtag cgtcatcgtc
    14221 acgtgtgatc tcaaagtctt tttcagtctc agcaaagcca tagtaagctt cttcatccac
    14281 cagatcagct tcatcatata ataagaattc gtctgtttta gcgagtaatt ctgccgttgc
    14341 ttctaacaag ttctctaagc cttgatgtgc taggcttgaa atagggaaaa tcattggcaa
    14401 atcattaaac tcgtcatatt gggtagccaa ttttttcttg aaggctttca agttttcttg
    14461 agcctctggc atgtccattt tattagccac aataatctgt gggcgctcca tcaatcgcaa
    14521 attataggtt tccagctcgt tattaatgga aacataatct tcgtaaggat cacgaccctc
    14581 actagcagac atgtcaatca catgaagaat aacccgtgtc cgctcgatat gacggaggaa
    14641 ttgagttccc aaaccaaccc cttggctagc accttcaatc aaacctggca gatctgccat
    14701 agcaaagcta tcacctgact ttgtacgaac cattccaaga ttaggaacaa tagtggtaaa
    14761 atgataggca ccaattttcg gtttagctga tgacacaaca cttaacaagg ttgatttccc
    14821 aacagatggg aatccgacca agccaacgtc agctaagatt ttgagttcta actctaactg
    14881 acgttcctca cctggctcac cattttcagc aatctctggc gcaggattac gaggagttgc
    14941 aaaacgaata ttcccacggc caccacgacc acccttagca atgaccactt cttgaccatg
    15001 ttctactaag tctgtaatta cttttccagt ttcagcatca cgaaccgttg tcccttgagg
    15061 aacaaagaca atcaaatctt ctgcaccacg gccgtgcatg cctttggtca tacctttttc
    15121 accagatttc gccttgaatt tacgattata ccggaaatcc atcaaggtac gcaaaccttc
    15181 gtccactcgg aagataactg agccaccttt accaccgtct cctccccaag gaccgccatt
    15241 tgggacgtac ttttcacgac gaaaagctac cataccatcg ccaccacggc ctgcttggac
    15301 actgattttt gccgtatcta aaaacatact catacttttt ttattctcta actttctttt
    15361 actttaaaaa aacgcctttc agcgtttcct tacattaata ctgatagggc actcgcaata
    15421 agcccgccaa cagttacaag aaccattagg ataacaacga ccattgtcac tttttcaaag
    15481 gttgtttttt tgcgaggtcc attttctcca aatgccacgt taaatacctc atctattgat
    15541 ttttcttctt atatcttaac atgaaagatc gttttaggca aatatcagat gaatatttac
    15601 gctatgtagg ctattactga gtattgtatc ttgtgaattt catatgaata tgatacatta
    15661 gcatttgcaa aataatgtta cagataaaaa cctcctcgtc tggtgaactg caccccaaaa
    15721 gttagacaca aaatctaacg attggggtgc tattttttta tgaaatttaa tcaagaaacg
    15781 aaagttaaga tttatgagtt acggcaaatg ggagagtcca ttaaatccat atcaaaaaag
    15841 tttgatatgg cagaatccga tctcaaatat atgattcgct tgattgatag gtatggggta
    15901 accattgttc aaaaatgtaa gaataattat tattctccag aactgaagca agagataata
    15961 aataaagtcc tgattgacgg ccaatctcag aaacaaacat ccttagatta tgctttacca
    16021 acttctagta tgctctcaag gtgg
//
LOCUS       DAUCWO010000037        16004 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUCWO010000037 DAUCWO010000000
VERSION     DAUCWO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16004
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.037"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(155..2419)
                     /gene="glgP"
                     /locus_tag="VGY20_001136"
     CDS             complement(155..2419)
                     /gene="glgP"
                     /locus_tag="VGY20_001136"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ4273991.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSIASLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDQSWLVPTNI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(2454..3947)
                     /gene="malQ"
                     /locus_tag="VGY20_001137"
     CDS             complement(2454..3947)
                     /gene="malQ"
                     /locus_tag="VGY20_001137"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ4273992.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEATDLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(4062..5081)
                     /locus_tag="VGY20_001138"
     CDS             complement(4062..5081)
                     /locus_tag="VGY20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4273993.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESV
                     RDISH"
     gene            5325..6572
                     /locus_tag="VGY20_001139"
     CDS             5325..6572
                     /locus_tag="VGY20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4273994.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            6846..8207
                     /locus_tag="VGY20_001140"
     CDS             6846..8207
                     /locus_tag="VGY20_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4273995.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            8207..9043
                     /locus_tag="VGY20_001141"
     CDS             8207..9043
                     /locus_tag="VGY20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4273996.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            9230..10046
                     /locus_tag="VGY20_001142"
                     /pseudo
     CDS             9230..10046
                     /locus_tag="VGY20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10518..11501)
                     /locus_tag="VGY20_001143"
     CDS             complement(10518..11501)
                     /locus_tag="VGY20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4273997.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(11532..12782)
                     /gene="dltD"
                     /locus_tag="VGY20_001144"
     CDS             complement(11532..12782)
                     /gene="dltD"
                     /locus_tag="VGY20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ4273998.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(12775..13014)
                     /gene="dltC"
                     /locus_tag="VGY20_001145"
     CDS             complement(12775..13014)
                     /gene="dltC"
                     /locus_tag="VGY20_001145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ4273999.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(13032..14288)
                     /gene="dltB"
                     /locus_tag="VGY20_001146"
     CDS             complement(13032..14288)
                     /gene="dltB"
                     /locus_tag="VGY20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ4274000.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVVPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(14285..15823)
                     /gene="dltA"
                     /locus_tag="VGY20_001147"
     CDS             complement(14285..15823)
                     /gene="dltA"
                     /locus_tag="VGY20_001147"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ4274001.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(15835..15978)
                     /locus_tag="VGY20_001148"
     CDS             complement(15835..15978)
                     /locus_tag="VGY20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ4274002.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         5078 a         3165 c         2730 g         5031 t
ORIGIN      
        1 ttacctccat agatcgctaa acagacttgc tttacgctta atgtaataat taacctaagt
       61 ctgaagtaaa atcataagac agcagaaact actgtcttat aatttagttt aagcatgtta
      121 cggtaccgct accccacatt gcaattatta ttttttatcg tgaatgccaa atatcttcgt
      181 tatactgctc aatggtacgg tcagatgaga agaatccagc ttttgcgatg ttatggacca
      241 cttttgtcat ccataaatct tggtcttcat agtctgctaa cattttttct ttcacttcaa
      301 tgtactcttc aaggtcaatc aaagtcatga accagtcttt tgaaatcaat tctttataaa
      361 gacgatcaag acgttcttcg ttgccaaatg ctagcaattc tggactgata ataaagttca
      421 ctgctgcttt aatagcaggg tggttatcat agtaatcttt tgctacataa gaagcagtcg
      481 cataaaggtt gatgatggtg tcagaatctt taccaaaggt atagatattc tccatgcctg
      541 caagctcagc gatttctacg ttagcaccgt ccattgtacc aagtgttaaa gcaccgttaa
      601 gcatgaattt catattacca gttccagaag cttctttgga tgctagtgaa atttgctcag
      661 aaatatcagt tgctggaatc aagtgctctg ctactgtcac gttgtaattt tcaactagat
      721 gcacattaag gtatgggctt acttcagggt cattgttaat caattcagac aagcaaagga
      781 tcaagtgaat gatgtcttga gcaatgatgt aagcaggcgc tgctttacct ccaaagataa
      841 cagtgatttt acgttttgga aggttgcctt ttttaatttc caaatatttg tgaatcacgt
      901 aaagagcatt catctgttga cgtttgtact cgtggaaacg tttgatttgg gtatcaataa
      961 tagaatgttc atcaagctca atgtctttat tgtctttaag gtaacgttta agagctaatt
     1021 tgttgttgaa tttgatttca gccaatttag catgaacagc tttgtcatct gcaaaggcca
     1081 tcaatttttc taattttgtt gcgtcagtca agtactcatc gccaataagt tctttaatgt
     1141 aatcagccaa gtcttggtta gcaaattcta gccaacgacg gaaagtaatc ccgttggttt
     1201 tgttgttgaa tttttctggg taaaggtcat agaaagcttt taattcgctg tttttcaaga
     1261 tttctgtgtg gagtgctgct accccattga cacttgttgc aaaatgaata tccatatggg
     1321 ccatgtgcac acgacctgat tcgtcaataa tttgaacagc tggatcagac acttctgaac
     1381 gaacaagagc atccaatttt tcaatgatgg ttaccaagtg tggcactact tcgtttaagt
     1441 aagcagttgg ccatttttca agggcttctg caagaatagt gtggttagtg taaccaacca
     1501 tatttttcac aacagctacc gcttcatcaa agtcaaagcc atgtttttca gtcaagagac
     1561 gaattaattc tgggatgacc attgacgggt gagtgtcatt gatttgcacg taagcgtagt
     1621 ctgcaaggtc atgaaggttt gaaccacgtt cgatagcttc atcaatgatt aattgcgctg
     1681 catttgacac cataaagtac tgttggtaaa tacggagcaa ttccccattt ttgtcagaat
     1741 cgtctgggta gaggaacaag gtcaagtttt tagcaatttg cgttttatca aatgaaatac
     1801 cgtctttgat taacccgtaa tcaacgccct cgatatcaaa caagttaagg tagtttttag
     1861 tgtcgcgttt gtaacccaaa acatcaatac gatcaagacg agatttcaag gtgaagttct
     1921 taaaaggcac atcatacgaa atgtttgtag gaaccaacca tgactggtct tcaatccagt
     1981 agtttggttc agcttcttgc tcattgtgtt tgaagacttg cttaaacaac ccacaatgat
     2041 agtttaaacc aaccccttca ccattaatcc caagagatgc aatagaatca atgaaacatg
     2101 atgccaaacg ccctaaacca ccattaccta gtgatggttc taattcaaca tcttcgactt
     2161 ctgcgataga tttgccagca gctgccaatt cttctttgat gtctttgtaa attcctaggt
     2221 taatcaggtt gtttgatagt aatttaccaa tcaaaaactc tgctgagata tagtaaactt
     2281 tgcgtttagc agaattttta gccttgtggc tagcttcttc tttgacaaag tttaataatg
     2341 atagataaat ctcttcattg ctggcttgcg taagtgattt tcctagttta gtttcaacat
     2401 attctgtaaa gcgtgtcatg tcttcctctt ttcaataatg taacatttaa atattatctt
     2461 gcgcgaccat aaagatctgt taaataaata aggtaatctt tgtgatcttg gttcaattca
     2521 ccgtcaagca tacgccattg ccaattacct ccaattgtat ttggcatatt catacgactc
     2581 ttaccatctt tatctaataa atcttggatg caaagaatgg ctgtatcaca aacagaagca
     2641 aagattgttc ttagcatggc ttttgtaata ctttcatctg catgtttgtg aagatagtta
     2701 tttacaaagt gtacttgttc ttcactaaga ttgtcatacc atccgttaac cacctcgtta
     2761 tcatgagtcc ctgtgtaagc gacacaatta cggtcatagt aatgtggtaa atctatactt
     2821 tgactcgtta tatcaaataa accaaattct aatatcttca tgccaggaaa gcctgttgat
     2881 gctaatagct gctctgcttt ttcatcaata taaccaaggt tttctgcgat gataggaagc
     2941 tctcctaagg cctcacgaac cgcagaaaat aacgctatac ctggggctga ggcccaatgg
     3001 ccatttctag cagttttatc accagcagga atttcccaaa aatctgagaa gcctttaaaa
     3061 tgatcgattc ttagctgatc atatagtttg aaactttctt gaatacgata aatccaccat
     3121 gcaaaattag acttttcatg agcagaccaa ttgtaggttg gatttcccca cagctgacca
     3181 tcttcactaa agccatctgc tggtacacct gcaataaaca atggcttttt atcactatct
     3241 actttaaata gctcaggcat cgtccaaact tctacactgt ccgcagaaac ataaataggc
     3301 atatcaccaa taatttctat ccctttatgg ttagcgtaag ttttcaaggc tgaccattgt
     3361 tggtaaaaga agtattgaca gactttatga taggtaatct tttttgcaag caactcacgg
     3421 taattattaa gtgagtcttc ttggcgttta atgacagttt cgtcgtccca atcttgtaag
     3481 gctttattgt taaaatattc ttttaaagcc ataaattcag caaaatcagt taaccagcta
     3541 gaagctgttt caaaagcttc taatttacac acattttctt gttcagcaac aaaagctctc
     3601 actgcttttt ctaaaagtgg acgcctaact tgaaaaagct gagcatagtc tactgcttca
     3661 ggatttgtgc cgaatgtaat atcacacaaa tctgttgctt ctaaaagttc atcatctact
     3721 agcaattcaa aatcaatgaa atgtgtgttc ccagcaatag ctgaaaatga ctgataagga
     3781 gaatctccaa aactcgtcgt tgttaaaggc agaatttgcc aataggtttg tttcgtctct
     3841 gctagaaaat caacaaattc aaaagctgat tttccaaaag tgcctatccc aaatttccca
     3901 ggcagggaac taatatgcat taagataccg cttgcacgtt tgttcatgat ttttctcctt
     3961 gtgaaatcta ttcatgatga gattataacg caagcgcttg cgtaagtcaa ccagtttcct
     4021 aaaaaaagta acattcctta aaaatgctac tttttttgtt cttaatgact aatatcacgg
     4081 acgctttctc tttgttttaa actaaaagga acaaaaatag tctgactaag attctgttca
     4141 ttggaattga taatatcaag taattgctta aaagacattt ttccaagatt ttcaacattt
     4201 atatcaaatg tcgttaaata agggtgaatt aatttagcgt aattagaatt attaaaagtg
     4261 ataattgaca tatcatctgg cacttttata tcataaaaag ataataactg taccatacga
     4321 atagccagaa catcaccaac aacaaccaag gcagttgctt taaaggaaga tatagtttcc
     4381 atcaatattt ctacgctaac aggatcctta cgatcaaata acagcattgg cttcgttttt
     4441 aaagatcgct taaaacaacc cttcaaatag ccaagataac gctcagaagt tacttctgat
     4501 agtaaatcat ctgttataaa taatatgttg tgatggcctt tttgataaag gtgttctact
     4561 gctgttttag ccattagctg attatcatta tcaatataag tggttttatt agtgtcacct
     4621 tgtggagcac ctacaatgac aaatggtaag ttatttgtca tcaaatattt tttgacagga
     4681 tcatcttgat cagaataaag aataataaaa ccatcaactc gtttttgaag atgcatcagt
     4741 ttcacttgct ctatcaggtc atcaagagat atacctgtag caatcgacac agtaaaatga
     4801 gattctttag cttcgttagt aatagtcgac aaaatttcca taaagaaagg ttcacttaat
     4861 ctatcgctag ttgtaatagg aggaaaaaca agtcctatat tgtgtgtcaa accactcgct
     4921 agaatctgtg ctgcgacatt tggaacatat cccaaatctg ccatcgcttt tctaaccttc
     4981 tcttttgttt tcatagaaat agatcgatta tcttttaata ccctactcac cgtcgaaggg
     5041 ttaacacctg ccttttgagc gacatctttt attgttacca tattaacctc gttactatat
     5101 ttaccaaaga taaaccttat catgggtttg tcatgtattt cgtttccatt atacaattat
     5161 ctttcaaaaa atcaacttca atcaagattc ccttgtcatt ttaactcaaa ttctctaaac
     5221 tattttttaa aaaaaacgac ctttttttaa aaaaaggctt gactttttct gcaagcggtt
     5281 gcataatatt aagtgtaaga aatatacctt aggaggaaat agctatgaaa tcatggcaaa
     5341 aagttatcgt cggcggagca agtttgacac ttgcaagtac cttgttagtg ggatgtggat
     5401 caggttcaaa agataaaaag gaagctggcg ctgactcaaa gactattaaa ctttgggtac
     5461 cgactggctc taaaaaatca tatgcagata ccattgcaaa atttgaaaaa gattctggtt
     5521 acaccgttaa agtggttgaa tcagaagatc caaaagcaca agaaaaaatc aaaaaagatg
     5581 cctcaactgc tgccgatgtc ttttcacttc ctcatgatca acttggtcaa cttgtagaat
     5641 ctggtactat tcaagaagtt ccagaaaagt acaataaaga aattgctgct acttctactg
     5701 atcaggcgct cgtcggtgca cagtacaaag ggaaaacata tgctttccca tttggtattg
     5761 aatcacaagt tcttttctac aataaatcaa aattagctgc tgaagacgtt acttcatatg
     5821 atacaattac tacaaaagca acttttggtg gtacctttaa acaagctaac acttacgcta
     5881 ctggtccatt attcatgtca gttggtaaca ctttatttgg ggaaaacggt gaagatgtaa
     5941 aaggtactaa ctggggcaat gaaaaaggcg ctgctgttct taaatggatc gcagaccaag
     6001 cttcaaataa aggttttgtt agcttagatg ctaacaacgt tatgtctaaa tttggtgacg
     6061 gttcagtagc ttcttttgaa tcaggaccgt gggactatga agctgctcaa aaagctatcg
     6121 gtaaagaaaa ccttggtgtt gctatttatc caaaagtaac tattggaggc gaaacggttc
     6181 aacaaaaagc attcttgggt gtaaaacttt acgcagtcaa ccaagcacca gctaaaggtg
     6241 atacaaaacg tatcgcagct agctacaaat tggcatccta cttgactaat gctgaaagcc
     6301 aagaaaacca attcaagact cgtaacattg tcccagccaa caaagaagtt caatcttcag
     6361 aagctgttca atcaaacgaa cttgctaaaa ccgttatcac tatgggttct tcttcagact
     6421 acactgtcgt aatgccaaaa cttagccaaa tgggcacatt ctggactgaa agtgctgcta
     6481 ttcttagtga tgccttcaac ggtaaaatca aagaaagcga ctaccttact aagttgcaac
     6541 aattcgacaa agatatcgct gcaacaaaat aatatgatct catgtcatca ctgctatact
     6601 aaaacaagca caaggtaagt acttctaaaa agttctttcc acttacctta tgaagagtgg
     6661 aactaggctc catcacattt tattcaagct tagtctcact cttacttgtt tttgttttac
     6721 tagtagctta gtgctactta aatttttaac tctttaaaag tttacgtaac acttagctat
     6781 ttcatcctaa caagagattg aatctgaact accactctag tatcctaaaa taaaggagaa
     6841 tctatatgac tcagtcaaac ttgacaccaa atatatctgt aatagaagct ttaaaaagag
     6901 gcagttggga tattaaactt tcgagtatta tcatgggatt tgccaacttt gctaacaaac
     6961 agtttatcaa aggaatctta tttttaataa gtgaacttat ctttctagta gcttttgtct
     7021 cccaaatcat ccctgctatc cgtggccttg tcactcttgg tactcaaact cagggaatga
     7081 ctacaaaaac tattgatggg attaacatcc aagttgctgt tgatggcgat aactctatgt
     7141 taatgcttat ttttggttta gcatcactca ttttctgcct agtctttgct tacatttact
     7201 ggtgtaatct caaaagtgct cgtaaccttt atcttttcaa acaaaaaggt cagaagatac
     7261 caagcttcaa agaagatctt gccacactta caaatggtcg ctttcatatg actttaatgg
     7321 ctatcccact gattggtgtg cttttattca ccattcttcc tttgatttac atgatctgtt
     7381 tggcctttac caattttgat cataatcact tgcctccaaa atctttattt gattgggtgg
     7441 gacttgctaa ctttggaaat gttttaagcg gccgtatggc aggaaccttt tttcctatct
     7501 tctcatggac tttgatttgg gctgtttttg ccacagtaac taatttcttc tttggtatta
     7561 ttcttgcttt gttaatcaat accaaaggat taaagtggaa aaaaatgtgg cgaaccatct
     7621 ttgttatcac cattgcagtt ccacaattca tctcattatt gattatgcgg aacttactca
     7681 atgacgaagg cccactgaat gctcttctca ataaaatcgg cttgattaat ggctcactgc
     7741 catttttatc tgatcctctt tgggcaaaat tctcaatcat ttttgtcaac atgtggatcg
     7801 gtattccatt taccatgttg attgcgactg gtattatcat gaaccttcca agtgaacaaa
     7861 ttgaggctgc tgaaattgat ggcgctagca aattccaagt cttcaagtct atcacattcc
     7921 ctcaaattct cttgatcatg acaccaaact tgattcaaca atttatcgga aatatcaata
     7981 actttaacgt catttacctc cttactggtg gtggtccaac aaattcagaa tactatcagg
     8041 caggaacaac agacttactg gtcacttggc tctataaatt aaccgtcaca gctgctgact
     8101 acaatttagc ttctgttatc ggtatcttaa tctttacagt ttcagctatc tttagcttac
     8161 ttgcttatac aaggacagca tcctacaagg aaggagcggc taaataatga aaaacaaacg
     8221 tcgatttcaa cttggacttg tttatgctac tttaatcatc ctctcaatca tttggctctt
     8281 cccaatcgct tgggttattc ttactagctt ccgtagcgaa ggaactgctt acgtcaatta
     8341 ttttattcct aagactttta cattaaatca ctacattaat ctttttacta atgaaacctt
     8401 tccttttgga aaatggttta tgaatactct tattgtagcc acttttacat gtattatttc
     8461 aaccttcata actgttgcca tagcctactc tcttagccga attaagttta agtttcgcaa
     8521 tggcttttta aaactagccc ttattttaaa tatgttccct ggtttcatga gcatgattgc
     8581 catctactat atcttaaagg ccttaggttt gacgcaaacc ctaacagcac ttgtccttgt
     8641 ttattcctca ggtgcagccc ttggttttta cattgcaaaa ggattttttg ataccattcc
     8701 ttattcactt gatgaatcag ctatgattga tggcgctact cgtatggata ttttctttaa
     8761 aataaccctt cctttagcaa aaccaatcat tgtctatact gcccttcttg cttttatggg
     8821 gccttggatt gactttatct ttgcacaagt tattttaggt gatgctacaa gtaaatatac
     8881 cgttgctatt ggactatttt caatgcttca gccagatacc attaacaact ggtttatggc
     8941 cttcactgct ggttctgtct taattgccgt cccaatcact ctcctcttca tgtttatgca
     9001 aaaatactat gttgaaggca tcacaggtgg ttctgttaaa taagttacaa cataccaaac
     9061 cttctaaggg caagcccttt aggaggtttt tgataaataa ggccgtagat aatcatatta
     9121 gtcctcacat ttatcttcag atcccgcgtt gtaaaatgaa gtgcaacaaa aaagacatgt
     9181 tcgtctgata tactagaatt ccccaattca gtatggaaag cagatgaaca tgtctaatat
     9241 taattctacc agaaaatcct cctattctca tctttcagcc acagaacggg gtgaaattgc
     9301 tgcttatctt aaaatgggaa agaaacccgt tgagattgct cgacttctgg gtagtcaccg
     9361 ttctacaatc tgccgagaaa taaaacgtgg ttcagtagat caggtaaagg ataagaatgg
     9421 aaaacaaacc ttcttcaacg cctatttcgc tgatagtggg caacgtgtct attaaaccaa
     9481 tcgatttgcc taagatggtt agtatcagaa aacgttctaa gaaagtcatg aaaacgaata
     9541 agaagacttt aggtaaatct atcgaagaac gtccagaata tattaatgat cgttctgaat
     9601 ttgggcattg ggagattgat ttgattctcg gcaagaaaac caaaggtgaa gctgttatgt
     9661 tgactttggt agagcgccag acacgctatg cactaggagt taagctagaa gacaagcagt
     9721 cccaaaccat taaccgcgct gtcacgcatc tcatcagtca gtatcctatt gcatccatca
     9781 cagcagataa tggttctgag tttagtttac tctcaacctt agaagctgtt gacgtttact
     9841 ttgcacatcc ctattcttca catgagagag gaacaaacga gaacttcaat ggcctcctca
     9901 gagaatatgt ccctaaagga atctcactta atccactaac ctctgaagaa cttgacaatt
     9961 atcttacagc catcaatgag cgcccaagac gacttcttca atatcaatcc tcaacattcc
    10021 tgtttgagct agcccgaaca gcttaacctc tggaactcta gttatctatg aactcgttgc
    10081 acttgacttg acaacttgcg agagcatatc agtagttttt ctgctaattg aaaacagggt
    10141 atttactgtc acacaagtct gtcctttctt tttttctaca actttatttc gtcagcgtaa
    10201 ataaaacaaa agctctataa tctcttgaat gggtaaatcc actgtagaga ttatagagct
    10261 ttttgaatat caaaaaagtc ccataagatt tataatgaaa agcaaccaaa ctctcattag
    10321 aaagactctt atggaacata tcaatcatac cttacaacac atcggaatta aagacaaaaa
    10381 tatcactcta aacaatgttt tgaaggatga atctcctact gagctcgtta ctacttatca
    10441 gaaaggtaga taggaaatcc tgatgaagag tgtgtccaag ggattatcaa ttaggtctta
    10501 gattacaaaa tagtaattca ggtatgctct actgttttca acactagttc tgcaatttgt
    10561 tttcgagctt gcttagcttc tcttattgga aaaataacat acgcatgaat cattttttct
    10621 gcaataatca gctgattagc aattcgttgt cttgttaatt gatcatgtaa agacataatg
    10681 tctgggtaaa aaatctcttt tgtcccaaca aaggtcgtta tttcaggcaa atcctcataa
    10741 tcaccataca aaggactgac ataaggaagt tgcatattac cttgaccacc tgcccatagt
    10801 tcacctatat cacgaagacg acgagcatct aggtaagctt cttctgcttc aaaatccttg
    10861 taggcttgat ttttaaaact aacatctaac caaggagata taagaattaa ttgctttggc
    10921 tggggtgctg ttttagtatc tcgtatatac ttagcaaacg ccaaagccat acccccacca
    10981 gaagaatcac caatgacact aacttgactc ggtccattag acaagcaaaa ttgctggtaa
    11041 gcttcaacta atttaggata gacatcttga taagtagcat acggtacttt aggaaagtta
    11101 ggaaaaacaa ccttggcatt agtcagctgt ataagatgct taaccgtctt aaaatgaagt
    11161 ttttcgggtc gtcgtaaata gacactgcca taaagataat aaataacagg ctgatgactt
    11221 tgcttttgat cattaataat aaaaacttgc atcccgttca catcgtattc ttgagttgaa
    11281 aaacctacta tctcttcagg attatctaga tgataaggga tattacgatt acgctgcgtc
    11341 tcaaccccct tataattatc aactttttta gataacttat actttctata agcaaacatt
    11401 ttctcaacca accaacttga aaaggagcgc ttttgaccat attcaactat caaaatacct
    11461 agtatcactc caaaaaaaat caccacagca ataatgacca cttattatcc tcgttttcta
    11521 atattatttt actattgcaa gtctttccta tttgcccaaa ttttactata aaaataaggg
    11581 ttcattttat agttgggcac tggctgcttc gtttctagaa atggttgcac tttcttatca
    11641 aaagctaacc agccattcca accgagatgg atggtatctt gcataaagta ggactcacca
    11701 ccatcttttg agaagtcagc aatgcgatga aatccttgtg actttaactg gaattttatt
    11761 ttacgaactg ccgcttgata cttatcttga tttaagccgg tataatccgc ccaagcttta
    11821 ttaacaggag ttataacaaa gagtacatct gtttttcgtt tagcaaattc tgataataaa
    11881 agctgaaaat cattgtattc tggtgacgcc aggtaactat aattaacttg gaaattctta
    11941 taaagattgt atttaggtgc tattcgttta cgataaaatg tatttttaat cccaaaacgg
    12001 ttgttggttg ttgctaattg gcctctctta gttgctaatg cattcaattg tttatacgaa
    12061 aatactttag gtaatccctt aacgcgaggc aaaattcttt tttcatagtt agtagatttg
    12121 cctaaaaaac taaaaaggga ctcttctctc aatgctactt gatgttgaca tttcaaaata
    12181 gctctgtcta accgactaag agacttaccc ttacttactt ttttcaataa gtttgattta
    12241 gacacaccag ggttaagctc aagcaaacgc tttgctgcaa actgtgattc tttatcagtt
    12301 ctagctttta gtaaaaattc aatcacttga gtgttagaca agtacatctg aaccgcacta
    12361 ggattaatcc cttgagcagt aaaccattga ggagatacta caaagatggc ttttttcttt
    12421 tgcatttcat tggtaatttg ttgcatacca taataatgcg acaaagatgc tgatcctctc
    12481 ttaccaatta aaaatggtct atagctccgc ttgtagcgct ctgcaagcac cgaagggtgc
    12541 atactatcca ttcgactcca ttcgctagaa ccaaaaaaag gcacaaaacg acaagtctca
    12601 tctgataaag cttgtctttt aatcaaacca tttttaaaag aactatctgt gatcgcaacg
    12661 gcatttgctt tttcctgagc tatggaatga tcaagttgtg taggaaaact aaaaatagta
    12721 atcactacta aaacaaaggc aataagaaga ggacctagaa ttaaccagag tctcttaagc
    12781 atgttgcaat tcctctacac cttgaacaat cttagttact gtgttccaat catcacgacc
    12841 aaattctgaa ataggtactt taatattgaa cgttgactct aactcaacaa tcaattcgac
    12901 tgtcccaaga ctatccaaca cacctgcatc aaacaagtct tcgtccatca tctctgaaac
    12961 atcttccata aacaaacggt caaacaattc aattacagtt tcttcaatag acatttttat
    13021 acttcctttt attaatgtag tggtctgtta aaccaaaggt catttaaaaa accagagaat
    13081 aacagaagtg aaaacatgac aacgtggaaa gttaccacga tacataagac atggaatgcc
    13141 ctgctttgaa agaggggtga taagcctttt ttcttacggt gacggttgat ttcttttttc
    13201 ttacgaatcc aagcatcatt aataataagt cctataccat ggaaaagccc ataagcgata
    13261 taataccagg ttaagccatg ccaaaatccc atgactagca tattaaccag ataggcaaca
    13321 cctgaagtga catttcggtt tttaaaggtt ttatgcttaa tcaatagatg aaccaatcgc
    13381 ataaacacat aatctctaaa ccagaaggac aaactcatgt gccatctatt ccaaaaatct
    13441 ttcaaacttg cagataagaa aggcatgtta aagttttctg gtgttcgaat acccaatagg
    13501 taggaaatac caatcgcaaa catcgaataa ccagcaaagt caaaaaagag attcaagcca
    13561 tacacgtaca taaccagaat agtttcctta ttaaagtagc caccaaccat caaagcctta
    13621 ttttcaagga gaggaagtaa gatgccaccc aaacaatagg aaatgatatg cttgtataga
    13681 aaacctaaca tgagatacat gactgcctta ttgagcattg caaaataatc atcacgttca
    13741 ggaagtttat gataatcttc ctgaaaacgt ctgaagcgat caatggggcc acttgagaag
    13801 gtggggaaga aaatcatgaa gcgaataaaa tccggtaaac gaacctcttg gagagtcccg
    13861 tcacgcatct caataatcat tccaatactt ttaaaggtca aatatgaaat ccctagaaaa
    13921 ctaaagagtg tttgagaaga aggacctgtt aggggaacaa ctttgaccca gataaggggg
    13981 aataaggcca tcgcaatagc taagtaaaac actcctgcac tattagcttt ctcacggtat
    14041 ttctggtaag caaaaacact gatgatttgc cacaaaagat aaaagagaaa agcaactaat
    14101 tgttgataat gatctcctcc aaacataaag agaataaata ccaaactcac caaggtttca
    14161 taagtgctta agcgcttttg tttaaaaatc ccaatgatga ctggtaagaa agccaaaata
    14221 agatagacaa agtaaatggg gttaccgtaa ggttccatgt agggaatgtg gctaaaaaac
    14281 atcatcatcg gttattgacc tcattaatca aagttttaat atctatctta ccatttggag
    14341 ttaatggtag actatctcga taaagaaact tagaaggcat catataactc atcatatgat
    14401 ccttaacaga ggctttgata gctttggtta actccaattc cctatcaaaa cgctctttaa
    14461 ccccatcttt gacaacgata taggccaaga gattttgaac cttatgttct ttattgtacc
    14521 ttggaacagc cacagccgat gctaccattg gtgattgatt caactgttgg gaaacatctt
    14581 ctagttcaat acgataacca gcatatttaa tctgaaaatc caaacgccca ccatacagca
    14641 aaatattatc ttctgtcagt gagccaatgt cccctgtatg ataagcaggc tgtcctttaa
    14701 aagtaaagaa agcttctgcg gttttctcag gattgttgag gtaacctttt gacacagcag
    14761 gccctgtaac aataatttca ccctgctccc ctgaagctaa ctccttacca tcttcatcaa
    14821 taatataggt cggagaatct ggtttggggt agccaatcgg caagcgggta taattatcca
    14881 ccatttctct ggtaatttca atagcggata aggctacagt cgcttctgtt ggtccatagg
    14941 cattgatgat cttggcactc gggaaacgtt caaagagttt tcgagccgta gaaacggtta
    15001 attcttcgcc atcaaaataa aaatgcgtta aagctggcat cttagcctgg caaaaatcat
    15061 cgctaagcat ggccatatca gcaaatgaag gagtcgatgt ccaaatgcca actggaagtt
    15121 gcgcaatcgt tgtaaataac tgtttaaagt ctgccaccaa ttcttttgga agtgcaaata
    15181 aggttccacc taaagccaag gtaggtgccc agtacatgac agaaaggtca aaggaatagg
    15241 gtggctgcgc cagcatttgt ggctgtttag gtacatcaaa tgctgcatct tcgatcatcc
    15301 agttggtaaa gctcaaaaga ttgtcatgtg aaatttgcac cccttttggt tggcccgttg
    15361 ttccagaggt gaaaataatg taataattat catctccttt aacagaatgt gttcgctcat
    15421 agggcatttc tgctaatttt gctgactcaa tctctgatag tgagacaagg gaaatccctt
    15481 caatagtaag agggaattcc tcaatagcaa tgattaaact gggctttgca atctcaataa
    15541 tcgctaaaat acgttctggt gctgaatgca catctactgg gatataggca tgaccagatt
    15601 tggttaaagc tacaaaagta gctaacatat cataagtttg cgccccaaaa accaaaactg
    15661 gagattttgc cagtaaagct aagctatcta taaatgcagc aatgctatca gaatctcttt
    15721 tgagttgtcc gtaagtgcgg cgttccccta aacaatcata aactgggaaa tcagcctgtg
    15781 tctgagcaaa ctgctcaata ctgtcaatca tatccttaat catcagtcaa tctcctaaaa
    15841 ttcattataa ataaagttac tttgaccatg tcctagataa ccaaagaaat agaccagcaa
    15901 cattaaaatc agataaaata aaattgtctt gccaataaaa acagcaattc ctctcattct
    15961 tgatttgttt tttatcatac tttaccttct tgtattagtg aaat
//
LOCUS       DAUCWO010000038        15720 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUCWO010000038 DAUCWO010000000
VERSION     DAUCWO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.038"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            14..853
                     /locus_tag="VGY20_001149"
     CDS             14..853
                     /locus_tag="VGY20_001149"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ4274003.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            933..1430
                     /locus_tag="VGY20_001150"
     CDS             933..1430
                     /locus_tag="VGY20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ4274004.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            1450..1620
                     /locus_tag="VGY20_001151"
     CDS             1450..1620
                     /locus_tag="VGY20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274005.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLASLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            1750..2979
                     /gene="clpX"
                     /locus_tag="VGY20_001152"
     CDS             1750..2979
                     /gene="clpX"
                     /locus_tag="VGY20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ4274006.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            2989..3588
                     /locus_tag="VGY20_001153"
     CDS             2989..3588
                     /locus_tag="VGY20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ4274007.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDNKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            3736..4479
                     /locus_tag="VGY20_001154"
     CDS             3736..4479
                     /locus_tag="VGY20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274008.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(4537..6636)
                     /locus_tag="VGY20_001155"
     CDS             complement(4537..6636)
                     /locus_tag="VGY20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4274009.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQTQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNRAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQNDQ"
     gene            7014..7697
                     /gene="rpiA"
                     /locus_tag="VGY20_001156"
     CDS             7014..7697
                     /gene="rpiA"
                     /locus_tag="VGY20_001156"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ4274010.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPKAG"
     gene            7774..8985
                     /locus_tag="VGY20_001157"
     CDS             7774..8985
                     /locus_tag="VGY20_001157"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ4274011.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTIPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIFANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            9004..9444
                     /gene="arsC"
                     /locus_tag="VGY20_001158"
     CDS             9004..9444
                     /gene="arsC"
                     /locus_tag="VGY20_001158"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ4274012.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLADPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            9428..10237
                     /locus_tag="VGY20_001159"
     CDS             9428..10237
                     /locus_tag="VGY20_001159"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ4274013.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDTYTK
                     VYRDKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILAEL"
     gene            10903..11616
                     /gene="deoD"
                     /locus_tag="VGY20_001160"
     CDS             10903..11616
                     /gene="deoD"
                     /locus_tag="VGY20_001160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ4274014.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            11609..12397
                     /locus_tag="VGY20_001161"
     CDS             11609..12397
                     /locus_tag="VGY20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ4274015.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWINQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWQFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(12477..13382)
                     /locus_tag="VGY20_001162"
     CDS             complement(12477..13382)
                     /locus_tag="VGY20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4274016.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            13604..14140
                     /locus_tag="VGY20_001163"
     CDS             13604..14140
                     /locus_tag="VGY20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274017.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDLLYEHIQRVNLEE
                     EAFIKYYQGPKVPTGEFE"
     gene            14417..15109
                     /gene="pyrF"
                     /locus_tag="VGY20_001164"
     CDS             14417..15109
                     /gene="pyrF"
                     /locus_tag="VGY20_001164"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ4274018.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            15167..>15720
                     /locus_tag="VGY20_001165"
     CDS             15167..>15720
                     /locus_tag="VGY20_001165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ4274019.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAK"
BASE COUNT         4842 a         2765 c         3263 g         4850 t
ORIGIN      
        1 aaggttatta gagatgacaa aagctgacca gatttttaaa gctaatattc aaaaaattat
       61 aaacgaagga tcattgagtg agcaagcacg tcctaaatat aaagatggtc gaacagctca
      121 ttctaagtat attactgggg cgtttgctga atatgattta gctaaaggag agtttcccat
      181 cacaacttta cgacccattc caattaaatc tgctataaag gaattatttt ggatatatca
      241 agatcagtct aacagtttag atgtgctaga agctaaatat aatgttcatt attggaatga
      301 atgggaagtt gaccagacac gtactattgg acaacgttat ggtgctgtag tcaaaaaaca
      361 tgatatcatc tcaaaaatac taaagcaatt agcagaaaat ccttggaatc gtcgtaatgt
      421 catttctctc tgggactatg aagcatttga ggagacaaaa gggttgttgc catgtgcctt
      481 tcaaatcatg tttgatgtta gacgtgtagg ggaagatctt tatttagatg ctagtttgac
      541 gcaacgttct aatgacatat tagttgctca tcacatcaat gccatgcaat acgttgcctt
      601 gcagatgatg attgctaaac attttggatg gaaaataggt aagttctttt attttgttaa
      661 caatttacat atttatgaca accaatttga tcaggctcaa gaattactaa aacgacagcc
      721 agttgccagt cagcctaagc ttgttttaaa tgttccagat gggactaatt tctttgatat
      781 taaaccagat gattttgaat tacaaaatta tgatccagtg aagcctcagt tgcactttga
      841 ccttgctatt taaactttat ttagattttt tgtgagcatt ttgttcataa atttggtaaa
      901 ataaatagag tttaaaatga aggaaataat taatgacaaa ggaaattatt gctatttggg
      961 cagaggatga agcagggctt attggtgttg cgggtaagtt accatggtat ttaccaaaag
     1021 agttagaaca ttttaaaaag acaacgctac atcaagcaat cttaatgggc cgtgtgactt
     1081 ttgaaggaat gaattgcaaa agacttcctc aacgtcaaac ccttgtcatg acaagaaata
     1141 gagattatca agtagatgag gtgttgacaa tgactagtat tgaaaaagtc ttggagtggt
     1201 accatgcgca ggataaaaca ctttatatca ttggtgggaa taaggtttta gaagcgttta
     1261 atggttattt cgatcgtatc ataaaaacag ttatccatca tcgttttaaa ggagatacct
     1321 accgaccaaa tctagatttt agtcatttca cgcaagaaag ccaaacgttc tatgctcgag
     1381 acgctaaaaa tccttatgat tttaccgtta ctgctttaaa acacaaatag tctgaggaga
     1441 tgataagcga tgcaacgtag tatttttgga gtatttacgg cttttttgtg cgtgatttgt
     1501 gttctttgtg ccatcccagc ttttaaaaag aatcgttatg gcttagcaag tctgttctta
     1561 ttaaatgcgt ttactaatct ggttaatagt attcacgcct tctatggtac attgttttga
     1621 gatgcgactt gcaaagacag cactggtgaa gatttagcta tcaagcaaga atcaccaaaa
     1681 accaatacaa cacattgact aatattaaaa ttcagtaaaa gataagagaa agtaagagaa
     1741 ttataagaaa tggcaggaag tagaactaac gatattaaag tatactgttc attttgtggt
     1801 aaaagccagg atgacgttaa aaaaattatc gcaggaaata atgttttcat ctgcaatgaa
     1861 tgcgtagctt tatcacaaga aattataaaa gaagaattgg ctgaagaagt ccttgccgat
     1921 ttgacagaag ttccaaaacc aaaagagctc cttgatgttt taaatcaata tgtggttggt
     1981 caagatcgtg ctaaacgtgc tttatcagtt gctgtgtaca atcattataa gcgtgtctcg
     2041 tttacagaaa gtcgtgatga cgatgatgtg gatttacaaa agtccaacat tttgatgatt
     2101 ggaccaacag gttcaggaaa aaccttctta gcacaaactc tggctaaaag cctcaatgta
     2161 ccatttgcta ttgcagatgc aacttctttg actgaagcag ggtatgttgg agaagatgtg
     2221 gaaaatattt tactcaaact gattcaggct gctgactata atgtcgaacg agcagagcgt
     2281 ggtattattt atgttgatga aatagataag attgctaaaa aaggtgagaa cgtatctatc
     2341 acacgtgatg tatctggtga aggtgtacag caagcccttc ttaaaattat tgaaggtacg
     2401 gtagcaagtg ttcccccaca aggtgggcgg aagcacccta atcaagaaat gatccaaatt
     2461 gatacaaaaa atatcttgtt tattgttggc ggggcttttg atggcatcga ggagattgtt
     2521 aaacaacgtt taggtgaaaa agttattggt tttggtcaaa acagccgtaa aattgatgac
     2581 aatgcttctt atatgcaaga aattatttca gaagatattc aaaagtttgg gcttattcca
     2641 gaatttattg gccgcttacc agttgtggca gcccttgaac aactaaacac atcagatttg
     2701 attcaaattt tgacagaacc aagaaatgcc ctggtcaagc aatatcaggc ccttctttct
     2761 tacgatggcg tcgaattggc atttgataag gaagccttag aagccattgc caataaagcc
     2821 attgaacgta aaacaggagc acgtggtctt cgttctatta ttgaagaaac gatgttagac
     2881 attatgtttg aaattccaag ccaagaagat gtcacaaaag tgcgtatcac caaggctgct
     2941 gtcgaaggca agtcaaaacc cgttttagag acagcttaag gagaaataat ggctgaagaa
     3001 caagtcttaa atacccataa cgcctcaatt ttattgagcg ctgccaataa atctcattac
     3061 ccccaagatg atttgccaga aattgccctt gctggacgtt caaatgttgg taaatcaagt
     3121 tttattaata ccatattggg gcgcaaaaat ttggcaagaa cctcaagcaa gccaggaaaa
     3181 acccaactat taaatttttt caatattgat aacaagctta gatttgtgga tgtccctggt
     3241 tatggctatg ccaaggtttc taaaagtgaa cgggccaaat ggggaaagat gatcgaagaa
     3301 tacttaacca gcagagacaa tctgcgtgca gtcgtgagtt tggttgattt acgtcatgcc
     3361 ccttcaaaag aagatattca gatgtatgac tttttgaagt actatgacat tcctgttatt
     3421 gttgtggcaa ccaaagcaga taagattcct cgtggcaaat ggaacaaaca tgagtctgtg
     3481 gtcaagaaag ctttgaattt tgacaaatct gatactttta tagtattttc ttcggtagaa
     3541 cgtattggta tcgatgattc ttgggatgct attttagagc aggtctagcg ataggtgttg
     3601 atttatctat gctaaaacat tctcagtaga gggtgttttt cttatcgaaa acataaaaaa
     3661 tcatagttag gtttaccgat tttacttgac gccttaatga tgacatgcta gtattttata
     3721 ttaaggagat gagtcatgac acaagtagcc caagggcgct ctaaaggaaa gtcattgtta
     3781 tgccatcaat gcaatgccct ttttgtattt attatttttc ttatcttgct tgctgtactt
     3841 tttggtgttc gatatcgtaa tagctctatt gaaggtatct ggcgaacaac aagcattgat
     3901 cagaagttag gcgatgattt tgctaaacgt cttactggtt tgcatcagag ccctcttatt
     3961 gatgatagtt tattaacgtc atctcagatg atactaacag ttaaaaataa caacgttgac
     4021 ttgagtttta gtgttcaagt tgaacgagac atttttgtca aacgtttagc tgcttatcac
     4081 caaaatgaat tattaaagac attaaaggaa aatcatttag ttgttggtga cttgagtagt
     4141 aaagaacgcc aaattattga gaatagtatg ccagctagcc atgaattaga aatgatttta
     4201 gatcaagctt ttgagaaatt agcttctcag atcggaggca aatataatca aaaaacagga
     4261 catttatctg ctgttgttct caaaggaaaa gttaatcgta ttttgcacac cattgatatc
     4321 aaagaagaag tagcagcggg acatacttct ttttcaaaag gattgctcac acctaatggt
     4381 tattttgatt acaccagatt tggaaagaaa cttgagttat taggcgatga aaaaattatt
     4441 tttaaaaaag cgctaaaaaa aagtccgtca agtgtataac gtagtcacaa gacggatttt
     4501 ttcatgtttt gaaaattatt cagttgaaga agtttattac tgatcgtttt gcctaatcac
     4561 tagttgtcct tcttttagga ttgcctgaag ttttttgact tctggatggt caagataata
     4621 atcagttata cggtcacgaa tttcctgttc gataatacgt cgtaatggac gagctcccat
     4681 agcgcgattg taccctaaat ctatcaaatg agctttagcg tcatcagcaa tcgttaaact
     4741 aatacctttt tttgcggtgg tttgatttac ttcagctagc attaaatcaa caatttcttc
     4801 taaattttct ttttgcagat gattaaattt aatgatacta ttaaaacggt tcaaaaactc
     4861 tggtctgaaa taaggtgcaa tgcgatccat aatgttaaac ccagaagttt ccgtatcttg
     4921 ttggccaaaa ccggcatttg atgtagcaat aataacggta tttttaaagt taatagtatt
     4981 tccttgtcca tctgtcaatc gtccatcatc taacacttgt agaagaagtg taatgatttg
     5041 aggatcagct ttttcaattt catctagtaa gacaatagca taagggttac gacgaacacg
     5101 ctcagttagg gtattattat tatcatcgta gccaacataa ccagccgttg tcccaatcaa
     5161 ttttgacaca gcagtgcgat cgttatattc ggacatatca agtcgaataa tagcatcttt
     5221 ggaaccaaat aaatcaagtg ctaattgttt agctaactct gttttaccaa caccagttgg
     5281 acccacaaaa aggaaagaac ctattggacg gttaccatca tcaaaacctg ctcgatttcg
     5341 tcgaatagcg cgtgctactg ctgcaacagc ctcatcttgt ccgatgacat ggctttttaa
     5401 gcggctgcta attcctttga gcctttctaa atcattagtc cccatgttag agacaggaat
     5461 accagttaag cgctctacag actctgcgat atcattgact gtagcagtga cattttgggt
     5521 ttggttatgt tgctcaattt gcttttgtaa tgtttcaatc cgtgtttttg ctgctaaagc
     5581 tttttcaaag tcttctttgg cgacagcttt ttcttgcctc tctttttgtt tagctatttc
     5641 agtttcgagt gtttttaagt cagtgaccgg gtgttgcgca gctaaatgag cagctgtcat
     5701 atccagtaag tcaattgctt tatctggcag gctacgctgt gggatgtatt gaatagaata
     5761 atcaacagca gcttttagaa cattatcagg aagggtaata tggtggtgtt gttcataaag
     5821 attacgaata cccattaaaa tgtgaaaagt atcttcagct gatggtgcat tcactttaac
     5881 ttcattaaag cgacgagcga gagcagcatt ttttaaaata gtattgcggt attcgtcttg
     5941 agtagtagca ccaatcagtg ttaattctcc tcgtgacaag gctggtttaa gaatatctgc
     6001 aagtcccttt gagcctgaat cgctgcttgt ggcaccagcg ccaactattt gatgaatttc
     6061 atcaaaaaat aagatgatat tcccagcttc ttttacttct tgaatcaagt tttgaatggt
     6121 ctcttcaaag gaaccacgat actgtgtacc agcttcaaga ctagaaatat caattgatac
     6181 gatttccttg tttttaatag cagctgggac atctccatta acaatggctt gagctaaacc
     6241 ttcaatgact gcagttttac cgacaccagc atctccgaca aggacaggat tatttttagt
     6301 tcgtcgtgcc aagatttcag cggtatcttg aatttctttg ttacgaccga tgacggggtc
     6361 aagatgccct tgacgagctt cttgtgttaa gttagttccg agttgagtca atacactatc
     6421 agctttaggt gttaacattt gagaatttgt tgctttggtt gtcgctacag ggagttgtcc
     6481 tgtttgacga tagtgttgga attcttcggg agttatttcg cgaccgttaa cgagatagcg
     6541 gggattttcg ctacgataac cacccatgtt tgccattaat tggttgaaaa tatcatccat
     6601 atttacaaaa ggatctttac cagaaaaatg tgtcatatca gttacctcgc aataaaaaat
     6661 agactataag atgtttgtaa gcagtcgcag aatcgttgtt aggatttcac atgataggaa
     6721 accctttgct aataaaatga ttcagacatt atttttactc atcttatagt attattataa
     6781 tcaaaaggtc agtaaaggtc aaacaaaaac acttgatttt caaaaagcta aataaacacg
     6841 aaaagtaagt gcttatatga tgactttaga ataaatcata agttttaaaa agatcatcct
     6901 attctatagc acttaggctg tcaatgagag aaaaacaaat gttttcatta acaagttggc
     6961 gctttcatgg tataataaag tgctaagacg caattctatt ataaggggga atcatggaag
     7021 cactcaaaaa aattgccggt gttaccgctg cgcaatatgt gactgatggc atgaccattg
     7081 ggcttggaac tgggtcaacg gcttactatt ttgttgagga aattggccgt cgcgtaaagc
     7141 aagaaggact gcaagtggtt ggtgtgacta cgtcaagtgt gactagcaag caagcagaag
     7201 gcttaggcat tcctttaaaa tcgatagatg atattgatag cattgacctc accgtagatg
     7261 gtgctgatga agtggataag gatttcaatg gaatcaaggg tggcggcgca gccttactaa
     7321 tggaaaaaat tgttgcaaca ccaacaaaag aatacatctg ggttgttgat gcttctaaaa
     7381 tggtagagca tctgggtgcc tttaagttac cagtcgaagt cgttcaatat ggtgctgatc
     7441 gtttgttccg tgtctttgaa aaagctggtt acaaaccgtc ttttcgtatg aaaggtgata
     7501 gtcgtttggt gacagacatg caaaactata ttattgatct tgatttgggt tgtattaaag
     7561 atcctgttgc ttttggtcac ctgctagatg gaacagttgg tgttgtggaa cacggtctct
     7621 ttaatggtat ggttgataag gtgattgttg ctagtaaaga cggcgttact gttttagaag
     7681 cgcctaaagc aggctagtct tagcatataa cagtgttaat atattttata ggtattgggg
     7741 agtcaaccgt aacctcccct agattggaga cttatgtcaa aatttaatcg tattcacttg
     7801 gttgttttag actctgtcgg tattggtgca gcgccagatg ctgacaaatt ctttaatgct
     7861 ggtgtagctg atacagattc tgacaccctc ggtcatattt cagaaacagc aggcttatcg
     7921 gttccaaata tggcaaaaat tggacttgga aacatttctc gtccgatacc actaaaaact
     7981 attccaacag aagacaatcc tactggttac gtgaccaaat tggaagaagt atctcttgga
     8041 aaagatacca tgacaggcca ttgggaaatt atgggcttga acattacgga accttttgac
     8101 actttctgga atggtttccc agaagagatt ttaactaaga ttgaagaatt ctcaggtcgc
     8161 aaaattattc gtgaagctaa taaaccctat tcaggaactg cggttattga tgattttggt
     8221 ccgcgtcaaa tggaaacagg tgagttgatt gtttacacct cagcagaccc agtgcttcaa
     8281 attgcagccc atgaagacat tattcctgtg gaagagcttt acaagatttg tgaatatgct
     8341 cgctccatca cgcttgaacg ccctgccctt ttaggccgta ttattgcgcg tccttatgta
     8401 ggcgaaccag gaaactttac acgtacggct aaccgtcatg actatgcagt gtctcctttc
     8461 caagataccg ttttgaacaa actggctgac gcaggcgttc caacttatgc cgttggtaag
     8521 attaatgaca tctttaatgg ttcaggtatt acaaacgaca tgggacacaa taagtcgaac
     8581 agccatggta ttgacacgct tattaagact cttcaattac ctgagtttac taagggattc
     8641 tcctttacta acctcgttga ttttgatgcc aactttggtc atcgtcgtga cccagagggt
     8701 tatcgtgact gcttgcatga atttgacaat cgcctacctg aaatcttcgc aaatatgaaa
     8761 gaagacgatt tgcttctcat cactgctgac catggtaatg acccaaccta tgcaggcacc
     8821 gaccacactc gtgaatacat tccattgctt gcctactctg catcctttac tggaaatggt
     8881 cttattccac aagggcattt tgcggatatt tcagcaactg ttgcagaaaa ttttggagtt
     8941 gatacagcca tgattgggga gtcattcctt ggtcacttaa aataagaaag ttagtaattg
     9001 ataatggaag aaatcacgat ttatcataat ccaaactgtg gcacttcacg taatgttctt
     9061 gccatgattc gccatgcagg tattgagcct actattattg agtatttgca gacgccacct
     9121 aatcgggaga ctttaattga gctcttacaa tcgatgggaa tctctgcgcg agaactcttg
     9181 cgaaccaatg ttcctgaatt tgaggctcat ggattagcaa atcaagcagt agctgagaaa
     9241 gacgttatta atgccatgtt ggcggatcca attttgatca atcgtcctat tgttgtgact
     9301 cgcaaaggag taaaactttg ccgtccttct gagaccttac tggatattct tcctgttccc
     9361 ctgcctagtc cttatatgaa agaggatggc gagagtgtga accctattga ggtaaaagga
     9421 gaaaaagatg tcattgatga ctaaaattaa cgaaacaaaa gactttttag ttactaaagg
     9481 aattgaaact cccgaatttg gattaattct gggttcaggc cttggtgaac ttgctgaaga
     9541 agtagaaaat gctattgtta ttgattatgc agatattcca aactggggca agtcaacggt
     9601 tgttggccat gctggaaagt tagtttacgg agatttagct ggccgtaaag tgctcgcctt
     9661 acaaggacgt ttccattttt acgaaggaaa tccgcttgaa gtggtgactt tccctgttcg
     9721 tgtcatgaaa gctcttggat gtgaaggcgt tcttgtcaca aatgcagctg gtggtatcgg
     9781 ctacggccca ggtaccttga tggcaatcac agaccatatc aatatgaccg gaaataaccc
     9841 actaattggt gaaaacttgg acgagtttgg tccacgcttc cctgatatgt ctgataccta
     9901 tactaaggtt tatcgcgata aggcacatga agtggctgaa aaaatgaaca tcaaactaga
     9961 agatggtgtt tatatgggct tgactggtcc aacttatgaa acaccagcag aaatccgtgc
    10021 tttcaaagta ttaggagcag atgctgttgg catgtcaact gtaccagaag ttattgtggc
    10081 tgctcattct ggacttaaag tgcttggcat ttcagctatt acaaactttg cagctggttt
    10141 tcaatcagaa ttgaaccatg aagaagttgt ggaagtgacc caacatatca aagaagattt
    10201 caaaggtctt gttaaagcta ttttagcgga gttgtaggac gaacaaaatc aactggagca
    10261 ttattacagg aatgttattt tatgtcgtca gtttggcaca tctgttcgca ataaaaaaat
    10321 caaaaaataa tcacggagtg tgagttccat gaatgttgaa ctcagattct agcttttcta
    10381 actaacataa ccaacgaagg gaaagagacg tattcggttt aaattaagca tggactcgaa
    10441 catttcttcg tattaatttt taaataataa aggaaagaga gttagtcctt tcttatttga
    10501 actcgatttt tagctctctt cttctattca atatcaatta tgaaaggaat ggggttgtat
    10561 tcaattgaat acgggcggaa agctcggaaa ctagataaac taacttttgt gctcacacat
    10621 ggcgttagtt ccctaaattt cagttgcttt ctgtccgccc tctgaccttc tacaaaataa
    10681 aaggaaaggt agggctaact gtattcattg aattgaatac gggcggagaa ctgtttttct
    10741 tacttataaa aaataaaaaa ataagaaagg tgagggctga gttcattaat ttgaattcgg
    10801 gctaaggact tggtcaaaaa gataagttgt ctagaaaatc aggattttct gccaacttcc
    10861 tattttgcca tcgttctttt aacgccctca gtatcataat ttatgtctat ccatatttct
    10921 gccaaaaaag gcgatattgc tgataaaatt cttctacctg gagacccttt gcgcgcgaaa
    10981 tttatcgctg aaaatttcct agaagatgca gtgtgtttta acgaagtgcg taacatgttt
    11041 ggctacacag gaacttacaa agggcaccgc gtttctgtca tgggaactgg tatggggatg
    11101 ccttcgatct ctatctatgc tcgtgaatta atcgttgatt acggtgtcaa aacattgatc
    11161 cgtgttggta ctgcgggtgc gattgaccca gaagttcatg tgcgtgaatt ggtgcttgcc
    11221 caagcagcag caaccaactc aaacattatc cgtaatgact tcccagaatt tgatttccca
    11281 caaattgctg attttggttt acttgacaag gcttaccata tcgccagaga aatgggagta
    11341 acaactcacg ttgggaatgt gctttcttct gatgtctttt acactaacat gcctgagcgt
    11401 aatatggctc ttggtaaatt gggtgttaaa gccatcgaaa tggaagcagc agctctttac
    11461 tatttagctg ctcagcacca tgtcaaagct cttggtatta tgacaatttc tgacaatttg
    11521 aacgacccaa cagaagacac cactgctgaa gagcgccaaa caactttcac tgatatgatg
    11581 aaggtcggct tggaaacctt aattgccaat gactaataat caaacgctag acatcctttt
    11641 ggatgtctat gcttataatc acgcctttag aattgctaaa gccttgccaa atatccctaa
    11701 aactgctctc tatttactag agatgttaaa agagcgcaga gaattgaacc ttgcctttct
    11761 ggcggaacat gcagcagaga atcggaccat tgaagaccag taccactgtt cattatggat
    11821 taaccaatcg cttgaagatg agcagattgc caattacatt ttggatttag aagttaaagt
    11881 aaaaaacggt gctattattg atttcgtcag gtcagtgtcg cctattcttt accgactttt
    11941 tctcagacta atcacgtcag aaattccaaa cttcaaggct tatatttttg atacaaagaa
    12001 tgaccaatat gatacctggc agtttcaggc catgttggaa tctgatcacg aggttttcaa
    12061 ggcttacctg tctcaaaagc agtctcgcaa tgtgacgacc aaaagcttag cagacatgtt
    12121 gacgttgacc tccttacctc aggaaatcaa ggacttggtt tttttgttac gacattttga
    12181 aaaggctgtc cgtaatcctc tggctcattt gattaagcct tttgatgaag aggaactgca
    12241 tcgcaccact catttttctt ctcaggcttt tttggaaaac attatcacct tagcgacttt
    12301 ttctggtgta atctaccgac gtgagccttt ttactttgat gacatgaatg ccattattaa
    12361 aaaggagttg agcctttgga gacaatctat tgtctgatat ttatcaaaaa acacgataac
    12421 ttgaccgata caggtcttac taccgtgttt ttttagtatg acgattgaaa aagacattaa
    12481 tcaaaggtta cttcttcgag aaggtattcg ataaaacgtt cccccatttt ggacaagttg
    12541 gctttttcat gcttgataaa aacaatgtca atttcgtcag ggacatctag cgggatagcc
    12601 acaatctggt caccatttag attgctattt agaataccac tggcaacggt gtagccatct
    12661 aaaccaatca ttaagttaaa gagggtggca cggtcactga ccacaattga tttgttgtgg
    12721 ggcatctggg acatcatctc ttctgaaaaa taaaaggagt tgtgaatccc ttggtcatag
    12781 ctcaggtaag gaaaatctct caagtcgtcc atgcttaata ggcttttagt tgctagagga
    12841 tttgatttgc tgacaaaaat atgaggctgt gccttaaata atggagaggc agtcaaatga
    12901 ttgtcatcaa ataatttggt caagacatct cggttatagt cgttgataaa gagaacacca
    12961 atttcagatc gaaagttttt gacatcatca atgatttccc aagttcttgt ctcgcgcaaa
    13021 aagagttcgt accgagtcat gtcggtccgc ttgaggagtg agacaaatgc attgactacg
    13081 aaggcataat gttgagatga aacgctaaag agctctcggc cagtattgtg gtttttatag
    13141 cggtcttcta acagtgatgt ttgctctata atttggcgag cataagataa aaattccacc
    13201 ccatcttttg tcaaggtgat ccctttagga tttcggttga aaatggtaat gcccatctcc
    13261 atttccaaat ccttgaccgc attggataga cttggttgtg tgataaataa ttgcttagca
    13321 gcttcattca tagagccgca ctcaacaatt ttgataatat aatgcagttg ttgaattctc
    13381 ataagtacag tctagctcaa aaaaaataaa gacgcaagca caaacgtcat catgggttta
    13441 gaactttgaa aaagcgctgg agtcttttct caatatggcc taggataaat atgattcaat
    13501 tttgagagtt gaatctatca atttcattgc ccttttattt ttcttgtgtt atattaatag
    13561 tattaacaaa taggtatttt tctaatcaaa gggtggtggt cgtgtgaatg ttactgaaca
    13621 aaatagtcat caaatcttaa tccaaaaatt attggtcagt atccactatt tgactctttt
    13681 tcgagatgaa ctgaagcttg tagaacgaac tccttctata cttggagggg aatttccagc
    13741 tcatttagta cagtctgagt tgggagatat tgttgcggcc attgatacct tggatatgca
    13801 gcaacgcttg attgagtcta ccttttggta tgaagaatca gcttttaagt tgatgaacaa
    13861 aactttggat attgtcgata attggattaa aggagtggat catctgatag atttatgcca
    13921 gtccaaagaa gtctttcaaa ttattattgg agataaacgc attcgagttt ttggggtctt
    13981 aagtgatgtt ttttcatctt taaaagtgag tgctttgtca cttaaggaag ctcctattcc
    14041 ggatttgctc tatgaacata tccagagggt taatcttgaa gaagaagctt ttataaaata
    14101 ttaccaaggc cctaaggtac ccacaggtga gtttgaataa atgattgggt aaaaatgata
    14161 aaatgagttg acgtaagtgt acaaaaaaag atatagtagg aaaaatgtgt tcaaataaca
    14221 aaaagttttg acatcttgtt ttaaaaaagc tataatagtt taaatttaga cacctttaag
    14281 tgagtccaga gaggcaaaca aggtatacaa gataaaaaaa gggaattccc ttttagtttg
    14341 ctgtagcctc gcttctttgg tagcgaggct ttttggtact taatgagata ttgacacatt
    14401 atagaaagga taagttatga aagaagaacg cccaattatt gctttagact tttcatcatt
    14461 tgaggagact aaagcatttt tagacttatt tccagcagaa gaaaaactat acgtgaaaat
    14521 tggtatggaa ttatattatg cgcaaggacc agatattgtt cggtctatca aatcgttagg
    14581 gcataatgta tttttggatt taaaattaca tgatattcca aatactgttc gtgctgctat
    14641 ggcagttctt aaagaattag atattgatat ggcaactgtt catgcagctg gtggtgtaga
    14701 gatgctaaaa gcagctcgag aaggactagg ccaaggccct accttaattg cggtaacgca
    14761 gctaacttca accagtgaag accaaatgcg tggtgatcaa aatattcaaa cgagtctttt
    14821 agagtctgtt ttgcattaca gcaagggggc agctaaagct cagctagatg gggtggtttg
    14881 ttcggcacaa gaagtagaag ctatcaaggc ggtgacgcct acgggattta cttgcttaac
    14941 tcctggcatt cgtcctaaag gcagtaatat tggcgatcaa aaacgagtga tgactccaaa
    15001 ccaagcaaga cgcattggaa gtgattatat tgtggtaggg cgaccaatta cccaggcaaa
    15061 agacccagtg gctgcttacc aagccataaa agctgagtgg gcaggctaac agataagatt
    15121 aggaaaccaa cacttgtctg ttaaagaaga aaaaggagaa taagaaatga cactagcaag
    15181 ccaaattgca actcaattac ttgatattaa agctgtttat ttaaagcctg aagatccttt
    15241 tacgtgggca tctggcatta aatcacctat ttacactgat aatcgtgtca ccctttctta
    15301 tcctaagact cgtgatttaa ttgagaatgg gtttgtggaa actatcaggg ctcatttccc
    15361 agaagttgag gtcatcgctg gtacagctac agcaggtatt cctcacggtg ccattatagc
    15421 agataaaatg actcttcctt ttgcttatat tcgcagtaag ccaaaagatc atggagcagg
    15481 caaccagatt gaggggcgtg tgcttaaagg ccaaaaaatg gttatcattg aagatttgat
    15541 ttcaacaggt ggttcggtac tagatgcagc cgcagcagcc agtcgtgagg gagctgacgt
    15601 tctgggagtt gttgcgattt tcacttatga gttaccaaaa gctagtcaga attttaaaga
    15661 agctggtatc aagttgatta cgctttctaa ttatactgag ctaattgcag ttgcaaagtt
//
LOCUS       DAUCWO010000039        15593 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUCWO010000039 DAUCWO010000000
VERSION     DAUCWO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15593
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.039"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            13..750
                     /locus_tag="VGY20_001166"
     CDS             13..750
                     /locus_tag="VGY20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4274020.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERDIDELKKQAIEDKEATTAIEAASSDALEALADQA
                     DTLQSEEAAAVQSDNAASDALEALADQADALQSEEAAVVKADNAASDALEALADQADA
                     LQSEEAEVVQSDNAASDAWEKAATPIALDVKKTKDTKPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAIMVSTGVLVVSSKCKEN"
     gene            988..1530
                     /locus_tag="VGY20_001167"
     CDS             988..1530
                     /locus_tag="VGY20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4274021.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1539..2822
                     /locus_tag="VGY20_001168"
     CDS             1539..2822
                     /locus_tag="VGY20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ4274022.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2838..3698
                     /locus_tag="VGY20_001169"
     CDS             2838..3698
                     /locus_tag="VGY20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ4274023.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3700..4665
                     /locus_tag="VGY20_001170"
     CDS             3700..4665
                     /locus_tag="VGY20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ4274024.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4776..6059
                     /gene="aroA"
                     /locus_tag="VGY20_001171"
     CDS             4776..6059
                     /gene="aroA"
                     /locus_tag="VGY20_001171"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ4274025.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6052..6543
                     /locus_tag="VGY20_001172"
     CDS             6052..6543
                     /locus_tag="VGY20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ4274026.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFTNDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6751..8202
                     /locus_tag="VGY20_001173"
     CDS             6751..8202
                     /locus_tag="VGY20_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ4274027.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDAPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8251..9642
                     /gene="rlmD"
                     /locus_tag="VGY20_001174"
     CDS             8251..9642
                     /gene="rlmD"
                     /locus_tag="VGY20_001174"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ4274028.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQT
                     HHVECVSLLEKRS"
     gene            9794..10575
                     /locus_tag="VGY20_001175"
                     /pseudo
     CDS             9794..10575
                     /locus_tag="VGY20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10660..11061
                     /locus_tag="VGY20_001176"
     CDS             10660..11061
                     /locus_tag="VGY20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ4274029.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(11166..12650)
                     /locus_tag="VGY20_001177"
     CDS             complement(11166..12650)
                     /locus_tag="VGY20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ4274030.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            13024..14244
                     /locus_tag="VGY20_001178"
     CDS             13024..14244
                     /locus_tag="VGY20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4274031.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            complement(14364..14729)
                     /locus_tag="VGY20_001179"
     CDS             complement(14364..14729)
                     /locus_tag="VGY20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ4274032.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14844..15575
                     /locus_tag="VGY20_001180"
     CDS             14844..15575
                     /locus_tag="VGY20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4274033.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
BASE COUNT         4827 a         2727 c         3329 g         4710 t
ORIGIN      
        1 aaaggagaaa caatgggaaa agaaataaaa gtgaaatact ttttgcgtag atcagctttt
       61 ggattagttg cggtgtcagc atcagtatta gtcggttcaa cagtatctgc tgttgactca
      121 cctatcgaac agcctcgaat tattccaaat ggcggaacct taactaatct tcttggcaat
      181 gctccagaaa aactggcatt acgtaatgaa gaaagagaca ttgatgaatt aaaaaaacaa
      241 gctattgagg ataaagaagc tacgacagct atagaagcag caagttcaga tgccttagaa
      301 gcattagcgg atcaagcaga cactttacaa tcagaagaag ctgcagcagt tcaatcagat
      361 aatgctgcta gtgacgcctt agaagcattg gcggatcaag cagacgcttt acaatcagaa
      421 gaagctgcgg ttgttaaagc ggataacgct gctagtgacg ccttagaagc attagcggat
      481 caagcagacg ctttacaatc agaagaagct gaagtagttc aatcagataa cgctgctagt
      541 gacgcctggg aaaaagcagc aactccaatc gctttagatg ttaagaaaac taaagataca
      601 aaacctgtag ttaaaaaaga agaaagacaa aacgttaata cccttcctac aactggtgaa
      661 gagtctaacc cattctttac agctgctgcg cttgcaataa tggtaagtac aggtgtgtta
      721 gttgtaagtt caaagtgcaa agaaaattag ttgctatttg ttctagcaaa tgaaacaagg
      781 gaatcgaaag attctctttt tctgatattt aaaaatgcta cagtttataa tagcttgaaa
      841 gactaaatgt tctcaaactc attagaacaa gctttaataa aactaaccca gttgtatcaa
      901 aggatagata aagactaaga ctgttataga atatttgcaa aagatttgtt ataatggacc
      961 caatacaggt attgtgaaag gtataatatg gatatttgga caaagcttgc agtgtttgct
     1021 ttttttgaga ctccgaaagt aatattacgg ccttttcgct atgaagatca ttgggatttt
     1081 tacagcatgg ttaacgacac taaaaacctt tattatgttt ttccagaaca aaaaactaag
     1141 gcagcaagtg actatctttt agtacatagt tttataaagt ttcctttagg tcagtgggca
     1201 atagaagata aagcaaccca ccaagtaata ggttctatta gaattgagca ttatgatgct
     1261 aaaacgcgtt gtgctgatat tggctatttt ttaaactatg ccttttgggg acaaggaatt
     1321 atgacagaag tcgtaataaa acttgtttat ttatcttttc acgaatttgg tctcaaaacg
     1381 ttgcgtatta taactcattt agaaaataag gccagccaga aagtagctaa aaaagcaggt
     1441 tttcaactaa aaacctgttt taaaggaagt gatcgtaata ctcataaaat ctgcatttat
     1501 aaaatgtacc aactaactaa tgataggtga taatcattat gagcaaacat caagatattt
     1561 tagattattt agaaaaactt gctattggta aaaaagtgag tgttagaagc atttcaaacc
     1621 acttaaaagt aagcgatgga acagcctacc gtgctattaa agaggcagaa aatcgaggta
     1681 ttgtcgaaac caaaccaaga agtggaactg ttcgtattga aaaaaaagga cgggttcgta
     1741 ttgaccgtct gacctattcc gaaattgctc ggatcagtga ttcggaagtg ctagcgggac
     1801 atgctggttt aggacatgaa tttagtaggt tttcaattgg tgctatgact caacaaaata
     1861 ttcgtcgtta cttggtcaaa ggagggttat taatagttgg ggatcgtgaa acgattcaac
     1921 tcttagctct tgaaaaccat aatgccattt tggtgacggg cggatttcca gtatccaaac
     1981 gtgttattga aatggcaaat aaccaacgaa tcccagttat ggtaactcat tatgatacct
     2041 ttactgtggc aacaatgatt aatcacgcgc tatctaatat tcgtattaaa acagatctca
     2101 aaacagttga acaggtaatg atacctatta cagattatgg ttacctttgc gaaaacagct
     2161 ccgttgaaga attcaataca ttaataaaaa aaacacgaca agtcaggttt cctgtattag
     2221 attataaaag aaaagtgatt ggtgtagtta gtatgcgtga tgtggtggat cagttgccaa
     2281 caactaagct taccaaggtc atgtctaaaa atccaataac ggctagacca aatactagtc
     2341 ttgctaatat tagccagaaa atgatctttg aagatttaaa catgttacct gtcactgacg
     2401 aagaaaacaa tttacttggt atgattactc gtagacaagc aatggaaaat ttgccaaatc
     2461 accaaccaaa taacccctat acttatagtg agcaaatact ttctaacctt gaagaaacgg
     2521 ttgattatta ccaagtagta gttgagccaa caatgattga tagtgctggt aacatgtcaa
     2581 acggtgttat ttcagagttc ttaaaggaaa ttagtatcag agccctcacg aaaaagcacc
     2641 aaaaaaatat cattatcgaa caaatgatgg tatacttttt acatgctatt caaattgagg
     2701 atgaattaaa aatatatcct aaaatcatta ctgaaaatag acgaagcagc accattgata
     2761 ttgaaatttt tgtggatgat caagtgattg ctaaagctat tattacaacg aaaataaatt
     2821 agaggagtaa caaaacaatg ataacattaa aatcagcccg tgaaatagaa gcgatggata
     2881 gagcaggaga cttcttagca ggtatccata ttggcttaag ggatattatt aagcctggtg
     2941 tggatatgtg ggaagtggaa gcatatgttc gtcgccgttg taaagaagac aacgttcttc
     3001 ctttgcaaat aggggttgat ggtcacatga tggactatcc ttatgcgacc tgttgtggtc
     3061 taaatgacga agtagcacat gcctttcccc gtcattacat tctgaaagaa ggggatttgc
     3121 ttaaagttga catggtactc agtgagcctt tggataaatc aattgttgat gtagcagctt
     3181 tagattttga taacgtgcct gagatgaaga aatggacagg atcttatacc ggtggtttag
     3241 ctgactcttg ttgggcttat gctgttggaa caccgtctga cgaaataaag caattaatgg
     3301 atgtcaccaa agaagccatg taccgtggta ttgaaaaagc agtaattggc aatcgtattg
     3361 gtgatattgg cgcagctgtt caagaatacg ctgaaagctt cggttatggt gtcgtgcgtg
     3421 atcttgttgg tcacggtgtt ggtcctacaa tgcatgaaga gccaatggta ccaaactatg
     3481 gaacagcagg tcgtggactt cgtctaaaag aaggcatggt cttgactgtt gagccaatga
     3541 ttaatacagg aacttgggaa attgataccg acatcaagac aggttgggct cataaaaccc
     3601 tagatggtgg tttatcttgc caatacgaac accaatttgt gattactaaa gatggtcccg
     3661 ttattttaac aagtcaagga gaagaaagaa cttactaaaa tggcagaaaa aaaatggttt
     3721 gataaggtac tgtccaagtg gcagtatgaa cccattcaag tctttatgag gcatttacaa
     3781 agtgcagaaa tggatttatc tgccattgcg gttgcgtatt accttatctt aacggccttt
     3841 ccattaattg ttattgcagc caatattttt ccttacctca atattgatat tgctgatttg
     3901 ttacgtctaa tgaagcaaaa tttgcctaaa gatattttta gaccggcttc tgcaattgta
     3961 gaaaatattt tctctaaacc gtctggaagt gttttgggag tagcgacttt gacaggatta
     4021 tggacaatgt ctcgaagttt gacatcgctg caaaaagcta ttaataaagc ctatggcgct
     4081 tctcagcatc gagatttctt tatcggacac ttggttggtt tgttgaccag tttaattatt
     4141 ttatttttat tagcctttgc cttaatcttt tcaacatttt ctaaagctgc tattcaagtg
     4201 ctggataagc attaccattt gagtgataat atcacaacga ttttcttact cctcattcaa
     4261 cccattacag ttttgattat ttttgttggc ttgatgctac tatatttttt attacccaat
     4321 gttaagatta aaaaaattcg ctacatttta ccaggaaccc tatttacatc ttttgtgatg
     4381 acatttttga gtaatctggt tggcaattac gttgtgtata atgttgagcg gatggtagat
     4441 attaaaatgt ttggttcagt tatgattttt atcatcatgc tatggtttat tttcttagca
     4501 agaatcctga ttttaggagc tatatttaat gcgacctatc aagaaatgtc cttggggaaa
     4561 ttagaaggtc gtagtggtga tatgatcgct atccttaaaa aaacacttgg gaatgattca
     4621 gatctatctc cttcacagtc tatcgaagat agtcacactg attgatacaa aaaagataat
     4681 aagtcgtgga gtgagaaact attttcttgc ttcatttttt atgaggatga atagaaaatc
     4741 attgatctta gcaaatagaa aggcagatga aacggatgaa attgcgaaca aatgcaggac
     4801 cgttacaggg aacgattcaa gttccaggag acaagtccat tagtcaccgg gcagtcatat
     4861 taggagccgt tgctaaaggg gaaacacgag taaaaggact cttaaaagga gaagatgtgc
     4921 tttccacgat tcaagccttt aggaatttgg gggtccgtat cgaagaaaaa gatgaccagc
     4981 ttgtgataga aggtcaaggt tttcaaggat tgaccgctcc ttgtcagaca cttaatatgg
     5041 gaaattctgg aacctctatg cgcctcattg ctggtttatt ggcaggtcaa ccttttagtg
     5101 tcaaaatgat tggcgatgaa agcttatcca aacgtcctat ggatcggatt gtttatccgt
     5161 taaagcaaat gggggttgag atatctggag aaacagatag gcaatttcct ccactacaat
     5221 tgcaaggtaa tcgtaattta caaccaatca catatacttt gcctatctct tctgctcagg
     5281 ttaagtcagc tattttgtta gcagctttgc aagcaaaagg aacaacacag gttgttgaaa
     5341 aagagattac tcgaaaccat acagaagaaa tgattcaaca atttggtgga cgattgatag
     5401 ttgatggtaa acgcattact ttggtgggac cccagcagct cactgctcaa gagataactg
     5461 tccccggaga tatttctagt gcagcttttt ggcttgtcgc cggcttaatt attcctggtt
     5521 cggagctcct tttaaagaat gttggagtca atccgacacg aacagggatt cttgaagtgg
     5581 ttgagaaaat gggagcacag attgtctatg aagatatgaa taagaaagag caagtaacta
     5641 gtattcgagt ggtatattct cacttgaaag gaactatcat atcaggaggt cttatccctc
     5701 ggttgattga tgaacttccc atcattgcac ttcttgcaac ccaagcacaa ggaacaacct
     5761 gtattaaaga tgctcaagaa ttacgagtca aagaaacaga tcgtattcaa gtagtaactg
     5821 atattcttaa cagtatggga gctaatatca aagcgacagc agatggcatg attatcaaag
     5881 gccctacggt actatatggg gctaacacaa gtacctatgg agatcatcgt attggtatga
     5941 tgacagcaat tgcagccttg cttgttaaac aaggacaggt ccatttggat aaagaagaag
     6001 ccatcatgac gtcttatccg acgtttttca aagatttaga aaggctgtgt catgactaaa
     6061 gtgttattgg gatttatggg agtgggaaaa acaacggttt cgaaacacct aagtatgcac
     6121 tgtaaagata tggatgctat tattgaagct aaaatcggta tgtctattgc agcttttttt
     6181 gaacagcatg gagaaatagc atttcgaaca attgaaagtc aagtgttaaa agatctattg
     6241 tttacaaatg ataatagtgt catcgtaaca ggtggcggtg tagtggtttt acaagaaaat
     6301 cgacaattac tgagaaaaaa tcaccagcat aatattttat tggtagcttc ttttgaaact
     6361 ttgtaccagc gtttaaaaca tgataaaaag tctcaaagac ctctatttct taagtactct
     6421 aaggaagcat tttatgaatt ttatcaacaa cgtatggtat tttatgaggg actttcagat
     6481 ttagtaatca gagttgacca tcgtacgcct gaagaagttg ccaacatcat tgagggttat
     6541 tagcaggtca ttgtcgtaat ctgatattta tggttttaaa caggtatctg tctaaagtac
     6601 ttcttatggc aagttggctt tattaaatat taggaaataa tcttgattat tctataatga
     6661 ttaaaatttt gagaggacaa tctggctaat ataggttata atggtagtat tcgttaaaag
     6721 tgcaattgta atactaagag atgataagat atgacaaaat accccatggg tggccttagt
     6781 caccatgaag aattacgtta tttttatctc ctaaggaatt tgagttacct tagtgagaat
     6841 gaaaaaaaag agtttgcttt tttaaaatct aaattggaaa ttggtcgagc ttatgcgcct
     6901 tctaagcagc attataggaa gtcaaaacgg caagaacctt actttgaaga tgactactat
     6961 aacgactact caccaaatga tttactagaa gatgatgacg tcaatcatga ctcttctttt
     7021 gttccatatg gtttaccgat atatcctaaa gaagacagat atttaaacaa aaaaacaaaa
     7081 cttacagctc gtagacctat agatgctccg cagcctattg atgaagacga tgctttttta
     7141 acagaatctg ttgctcgttg tgctcttcct agatctcaaa aaagaaagca taagaaaaaa
     7201 ggatgtatga aatggttttt taacattctt ggcttattgc taatgactgt gcttatgggg
     7261 ttagggttga tgtttgcaaa aggggttttt gatatttcta ctaataaagc taattacaaa
     7321 ccagctgttt cccaagcttt tgatggtcaa gaaactcaag atggtacgaa cattctggtt
     7381 ttgggttctg accaacgagt tactcaaggt tctacagatg ctcgcacaga taccattatg
     7441 gttgtcaatg tcggcaacca tgctaaaaaa ataaaaatgg tctcttttat gcgtgacact
     7501 ttgattaaca ttccaggata cagttataat gataattcgt atgatcttaa attaaatagt
     7561 gcatttaatc ttggagagca agaagatcac cacggagcag agtacgttcg acgggcctta
     7621 aaacataatt ttgatatcga cattaagtat tatgtgatgg tagattttga aacatttgca
     7681 gaagctattg acaccttgtt tccaaatggt gttaagattg atgccaaatt tgcgacagta
     7741 ggtggtgtag ctgttgactc cgtagaagtt ccagatgacc tgcgaatgaa aaatggggtt
     7801 gttcccaacc aaaccatcga agttggagaa caacgcatgg atggtagaac cttgctcaac
     7861 tatgctcgtt tccgtaaaga tgacgaagga gatttcggac gaaccgtccg gcagcaacag
     7921 gttatgtcag ctgttatgtc ccaaatcaag gacccaacta aactctttac agggtctgcg
     7981 gctattggta aaatctatgc gctgacttcg accaatgttt cttttccatt tgttgttaaa
     8041 aatggtgttt ctgttttagg aagtgggaaa aatggagttg agcacgtgac catcccagaa
     8101 aatggggatt gggttgacga atacgatatg tatggaggac aagctcttta catagatttt
     8161 gataagtatc agaaaacgtt agccaaatta ggtttgcgct aagatatggt ataatggaga
     8221 gtaactaaag ttgctcttct tttttgagca atggttagcc ctagaaaagg aaaaagaata
     8281 agaatgttga aaaaaaatga tattatccaa gttgctattt cagatttaag ccatgaggga
     8341 gcaggtgtcg ctaaacacga tggttttgtt ttctttgttg ataatgcctt gccagaagaa
     8401 gtgattgaca tgcgtgtctt aaaggtcaat aaaaacagcg gttttggcaa agtagaagcc
     8461 tatcattacc tgtcgccagc tagaaatgca gatgtcaatt taacttattt gcgaacaggc
     8521 attgcagacc ttggacatct aacttatgaa gaccaactga ctttcaaaaa gaaacaagtt
     8581 caggacagcc tctataaaat tgctggaatc agtgacgtaa cagtcgaaag cacgattggg
     8641 atgacagaac ctttagctta ccgaaataag gctcaagttc ctgttagacg tgttaatggt
     8701 cagctagaaa caggcttttt ccgcaagcat tctcatgact tgattcctat ttcggattat
     8761 tatattcagg acaaagagat tgatcgtctc attaatttta cccgtgattt attacgccgc
     8821 tttgatatta aaccgtatga cgaaacagaa caaacaggtc tcttgagaaa tattgttgtt
     8881 cgccgaggcc attactctgg agaaatgatg ctggtcttag tgactacacg cccaaaagtt
     8941 ttccgtgttg accaagtcat tgaaaaaatc gttgaagctt ttccagcagt ggtgtctatt
     9001 attcaaaata ttaatgataa gaataccaat gcaatctttg gtaaagattt caaaacgctt
     9061 tacggcaagg ataccattac tgatagcatg cttggaaaca actatgccat ttctgcccag
     9121 tctttctacc aagtgaatac ggttatggca gaaaaactct atcaaacagc tatagccttt
     9181 tcagaccttt ctaaagatga cattgtgatt gacgcctact caggtatcgg aaccattggc
     9241 ctatcatttg ctaaaacagt taaagctgtt tatggtgttg aagtgattga agcagctgtt
     9301 agagatgctc aacacaatgc cgctcttaat ggcatcacca atgcctactt tgtcgcagat
     9361 accgcagaac atgccatggc aacatgggcg aaagatggca ttaaaccaag tgttattctg
     9421 gtagacccgc cacgaaaagg cttaacagaa agtttcatcc aagccagcgt ggcaatggga
     9481 ccacaaaaaa ttacctacgt ttcctgcaac ccagccacca tggcgcgtga catcaagcgt
     9541 tatcaagagt tgggttacaa actggccaaa gtacagcccg tagatttgtt tccccagacg
     9601 catcacgtgg agtgtgtaag tttacttgag aaacggagtt aaagagtccg cctcactctg
     9661 ttatttcaag gtgagacgct caaaaggtcc actggacctt ttgactcccg cagacgcatc
     9721 acgtcgagtg cgtagtgttg ctaatcaagg agtaaggtag ctgcctccac atgacaaaaa
     9781 taaggagaaa cgaatgccta ttaccaaaca cgagattccc cttttagaat gtgatagtaa
     9841 tccatcagcc gtcattatac ctacacatga aggtctacag ctaagtgtgt ctatgctttc
     9901 ttagaagacg agattgaagc ttttgcttta gaccatcaag gtaaaaaagt agcagaattc
     9961 atttcagcca caaaggttta ccctgtttat cagatacctt ataaaggcca agacatttgc
    10021 ctagctcaag ctcccgttgg ctcagctcct gcggcgcaat tcatggactg gctgattggt
    10081 tatggggtta aagaaatgat cagtacagga acctgtggcg ttttggtttc tatcgcagaa
    10141 aaccgttttt tagtgccagt taaggctttg cgagatgaag gaacaagtta tcattacgtg
    10201 gcaccatctc gttatattga tattgatcct aagatgctta gactgattga gaaaacgctt
    10261 ttagcacagg ggttagctta tcaagaggtc atcacctggt caacagatgg tttttaccga
    10321 gaaaccaaag aaaaagtggc ttatcgccag gaagaagggt gttcggttgt tgagatggaa
    10381 tgttctgcct tagcagcagt agctcaatta agaggcatcc tttgggggca actcttattt
    10441 acagcagata ctctagcaga tgtagaggtt tacgatcaac gcaactgggg tgctgattcc
    10501 tttagttttg cgcttcattt atgcttggaa gtgctcaata cgctagaaaa ggatggaaag
    10561 gctactgatt tttgaaacat gagctctgcc ttctgttcct atttgtagta taatgaggct
    10621 tgtattgctt ttagactaag cttagaaaag agaaaaacaa tgtcagattt aacacaagag
    10681 atgaccttaa atgttattag tatttttgat aattaccaaa ttgaacttgc tgaaaaagga
    10741 caccttattt taagtactga agtgactgag acggctttga attattatgg gaatgcccat
    10801 ggtggttatc ttttcaccct atgtgatcaa gtaggtggtt tggttgctag aactactggc
    10861 gtagaatctg tcaccttaca ggccaatgct aactatttaa aagcagggca caagggagac
    10921 aagctgatgg ttgaaggtcg attggttcat ggaggtcgaa ccacgcaggt ggttgatgtg
    10981 acgattcata accaaacagg agctcttcta acaaaaacaa gcttgaccat gtttgtgact
    11041 ggccgacgtc ataacgggtg attaatgaat caataaagga aaaaagtccc aactctctga
    11101 gaattgggac ttttttcctt tattgagaga taccataaat cacccgttag acggatggtt
    11161 acggtttatt tacggtaaag tttgtgatgt tggtaaacag ggtcaaaggt aatatttacc
    11221 ccaagttttc ggagtacatt tttatcttct tcggttaaga taacggtaga atgggcttca
    11281 ctacctttca aattgccgag ttctttcatg gctagattag cttcttcact agtcatagca
    11341 gtaatggcca acgcaatcag aatttcattg gaatgaagcc gcggattgtg actgcctaag
    11401 tggttcactt tgaggccttg aataggtttc acgtattcgg gttcaatcaa atgagtggtt
    11461 ttatcaatct ttgccaaggt tttaatcgca ttgatgataa cagctgcagt tggtccaaag
    11521 agttcagatg ttttacctgt cacaatctgc ccatttggta gttggagagc aagggcaggt
    11581 tggcctgttt gctctgcttt ttgatgagca gctacagtaa cgtgacgatc gtcaggggtg
    11641 acaccgatat cattcattaa gagctcaatt tttttgacag cagattctgt gactcgctct
    11701 gccttgaaat ccacgagggt ttggtaataa cgacggatga tttcttgttt agaagcttcg
    11761 atagcagctt cttcattgac gatagaaaaa ccaaccatgt taacacccat atcggttggt
    11821 gaggcataag gagacttgct caaaatacgc tcgaaggtcc gatttaagac aggaaagacc
    11881 tcgatatcac ggttgtaatt aacggccgtt ttgccataag tttccaaatg gaaggggtca
    11941 atcatattca catcatctag gtctgccgtg gctgcctcat aagccagatt gacagggtga
    12001 tgaagaggaa gattccaaac tgggaaggtt tcaaatttag catagccaga cttaacccca
    12061 ttgatttggt cgtgatacat ttgggacatg caagtggcta attttccaga cccagggcct
    12121 ggtgctgtaa caacgataag gttacgactc gttttgatgt aatcattttt ccccatcccc
    12181 tcagatgaga taatatgatt aatatctgtc gggtagccct tgataggata atgcaggtaa
    12241 gaagcaatgc catttttttc gagttgcttg cgaaaggcat ctgcagcggg ttggttattg
    12301 tattgagtaa taacgacaga acctacgtaa atatctaacg tgttaaattt gtcaattaag
    12361 cgaaagactt cttggtcata agaaatgcct aaatcaccac gcgctttaga atgttcaata
    12421 ttattagcgt taatggcaat tacaatttcg acttgttctt tgagctcttt gagtaattta
    12481 atcttattgt caggttcata gccaggaagt acacgggcag catggtaatc ttctaacatt
    12541 ttaccaccaa attccatgta aagtttgcca tcaaattggc tgatacgttc taaaatatga
    12601 tctctttgca agtttaaata cttgttagaa tcaaaagcaa tagttttcat tttttcacct
    12661 ctgtaaagca ttataacaaa gaaattccaa gaaagaaaag gttttttgca gggatacctc
    12721 attttgtgac tttttaaaaa aacttaggct tggttcttag aagaaaaatg tatccgctat
    12781 gttcaaaaga acttgacaat taaaatgagc tttactatac tagtaacaat taaatactac
    12841 tttaaacatg ttccgtgaag catgaaagtc ttaacattaa agagagtttt tttgtcatct
    12901 cttattattt agtgaacttt gatggacttg cggacttgtt tccaagtctt tttttgacct
    12961 ttggacaaag ggtattcaat taattaaggt agtcagtgag ctactattaa gaaggagaca
    13021 attatgtcaa cattatcatt ggacaccaca aataagcgag ctttagtggc agctattgca
    13081 gcttcaggaa cggatgactt gaatgtcatg tttcttgcct tttccatgtc ttccattatg
    13141 acagacttga gtcttagtgg cactcagggt ggctggattg cgaccattac caatttagga
    13201 atgttggtgg gaggcctctt attcgggttg ctggctgata ggcaccacaa gtttaaggtc
    13261 tttaaatgga cgattttact gttttcagta gcaacaggat taatttattt tacccagtct
    13321 ttgccctacc tgtacctgat gcgatttatt gctggcatag gagttggagg tgagtacggt
    13381 gtagccattg ccataatggc agggattgta ccaccagaaa aaatgggtcg gatgtcttct
    13441 ttaaatggta ttgctggcca gcttggctcc attagttcgg ctcttttagc agggtggctg
    13501 gctccaagtc taggctggcg tggccttttt ctctttggat tacttccgat tctcttggtg
    13561 atttggatga ctttagccat agatgaccaa aaaatttggg atcattatgg gcaagaggag
    13621 gaagaatgca gtcaaccaat taaaatcaat gaactattca aaacaaaatc cttaacagct
    13681 caaacccttg ctttaatggt catgacgact gtgcagattg cgggctattt tggaatgatg
    13741 aattggttgc caaccattat tcaaacaagc ttaaaccttt cagtaaaaag ttcgtccttg
    13801 tggatggtgg ctactattgt cggcatgtgt ttggggatgc tgtgttttgg tcagctttta
    13861 gattgctttg gtccacgtct gatttattcc ctctttttat tagcatcatc tatttgtgtt
    13921 tacctttttc aatttgccaa ttccatggcg agtatggtta ttggaggtgc tattgtcggt
    13981 ttttttgtca atgggatgtt tgctggctac ggggctatga ttaccagact ttatccccat
    14041 cacattcgat ccacggctaa taatgttatt ttgaatgttg gccgtgctct aggaggattt
    14101 tcgtccgttg ctattggtag tattttagat gcctcaggta tttcaatggt catgattttc
    14161 ctagcttctc tgtatgtgat tagttttgga gctatgtggt ctattggtca gttaaaagca
    14221 gaacgctacc aacaattgcg ttagcaaaga gatgggtgtt agtatttgag ttactttcac
    14281 tcacaaagct attttattga ttgagataac caaaaaggta agcacttagg gattgcttac
    14341 ctttttggtt cttttctttt gttttactcc gctactttgg ccataaaatg tttactgttc
    14401 accctaactc tagtatgaca ggcttttcca atgagttgtt ctcccacaaa agcttccaag
    14461 cggaaatcgt attttcggcc ttcttcttta agggctgtaa tgatgattgt aacaggatct
    14521 ccaatggctg aagctgccaa atgttggata gctatctcgc tgccaaccgt tgtaaagtca
    14581 ctgtcgagct gttcttgggc aaataagtaa gaagcatttt ccataaaagc aacaagagca
    14641 ggagtagcta ggacattcag cgttcctgag cccatggctt tagccgaatg attgggacgg
    14701 gtttcataag tgtgagaata aatagtcata agttaatctc ctcgtttcta taagcctatt
    14761 ataatatatt aagcgcttac agtcatgaaa ctactgtttt tttatgttat acttaataat
    14821 gaattttctt tgaaagggtt atgatgcgct tagataaatt tttagtggca actggtgttg
    14881 gaactaggag ccaagtcaaa cttctcttaa aaaagaaggc tattttcgtc aatcaaaagg
    14941 ttgaaacctc tgccaaagca cacattgatg aatacaagga tttggtgacg tatcaaggca
    15001 cgcccttggt ctatgaatcc tttgtttatt acctgttaaa caagccgtca gggtatgtgt
    15061 cagcaaccca ggacagacag caggctactg ttatggagtt gttagatgac acggctaggc
    15121 aaaaagcagt ttttcctgtg ggtaggttag ataaggatac ccgtggactt ttgctcttga
    15181 ccaataatgg acaactagcc catgaccttt tatcccctaa aaaacatgtt acgaaagagt
    15241 acttggctaa agtagtaggc atcatgactg aagctgataa agattatttt gctagaggta
    15301 tttccttaaa ggatcaccag tgcctacctg ctcatttaga agtgcttgct agtgatttac
    15361 agcagcaaac aagtttggtt aaaattacca tacaagaagg aaaattccat caggtgaaac
    15421 gcatggtagc agcctgtgga aagggagtgc ttgacttgca gcgtctcagc atgggaccat
    15481 taaaactaga ccctagcctt gcagaagggg agtttaggcg cctgacgcct gaggaattgc
    15541 aaagcctggc accttattgt cagagctcac tataaaaaga aacctcccct gtc
//
LOCUS       DAUCWO010000040        15319 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUCWO010000040 DAUCWO010000000
VERSION     DAUCWO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15319
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.040"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            464..781
                     /locus_tag="VGY20_001181"
     CDS             464..781
                     /locus_tag="VGY20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ4274034.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFMVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
     gene            905..2803
                     /gene="mnmG"
                     /locus_tag="VGY20_001182"
     CDS             905..2803
                     /gene="mnmG"
                     /locus_tag="VGY20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ4274035.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGYVAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            2893..4869
                     /locus_tag="VGY20_001183"
     CDS             2893..4869
                     /locus_tag="VGY20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ4274036.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            4866..5318
                     /gene="rplI"
                     /locus_tag="VGY20_001184"
     CDS             4866..5318
                     /gene="rplI"
                     /locus_tag="VGY20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ4274037.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            5348..6715
                     /gene="dnaB"
                     /locus_tag="VGY20_001185"
     CDS             5348..6715
                     /gene="dnaB"
                     /locus_tag="VGY20_001185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ4274038.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            6732..7004
                     /locus_tag="VGY20_001186"
     CDS             6732..7004
                     /locus_tag="VGY20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ4274039.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            7736..8347
                     /gene="rpsD"
                     /locus_tag="VGY20_001187"
     CDS             7736..8347
                     /gene="rpsD"
                     /locus_tag="VGY20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ4274040.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(8501..9034)
                     /locus_tag="VGY20_001188"
     CDS             complement(8501..9034)
                     /locus_tag="VGY20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4274041.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            9169..11442
                     /locus_tag="VGY20_001189"
     CDS             9169..11442
                     /locus_tag="VGY20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ4274042.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGDDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQST
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIV
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGTNALSSKSGELLDGSHQLSEGATKLADG
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(11558..12631)
                     /locus_tag="VGY20_001190"
     CDS             complement(11558..12631)
                     /locus_tag="VGY20_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEQ4274043.1"
                     /translation="MKNISRKCFMTSVVCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPLEIKIENETLHISEKP
                     RDVIITGGMELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTIQD
                     LEFSGGLTLLNTRIKKGKVFGTFDATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVEAEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGDVAVDITLTNPKTTAYKTKTEDGKISLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(12628..13215)
                     /locus_tag="VGY20_001191"
     CDS             complement(12628..13215)
                     /locus_tag="VGY20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ4274044.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGIAGILVAGVTLFESLTLIGSSIPAQAIGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(13202..13528)
                     /locus_tag="VGY20_001192"
     CDS             complement(13202..13528)
                     /locus_tag="VGY20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ4274045.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLQKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
     gene            13733..14374
                     /locus_tag="VGY20_001193"
                     /pseudo
     CDS             13733..14374
                     /locus_tag="VGY20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
     gene            complement(14421..15263)
                     /locus_tag="VGY20_001194"
     CDS             complement(14421..15263)
                     /locus_tag="VGY20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ4274046.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVTSGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMTFVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT         4936 a         2608 c         3068 g         4707 t
ORIGIN      
        1 aaattattgg aaatttctag ttatactttt atggttttgt tatcggcgtt cttacttgta
       61 gacttagaaa caacggtatc tgtattttac tactttagaa gatatcaaaa aatcgtatga
      121 taatactgat gacattttta tttctcgcct cgctagatta gacatagtga tacttttgta
      181 atcattttaa aaacattaca atagattgac agagctgtaa aaacattgac attactgtaa
      241 aggtctgtga tgaaatcaat attgacaatc actaatcatt tttttaaaat agatagtaac
      301 aatcggaatg atggtcaaag ctcatgaaag ttgtaggttt ttttgtcctg caatcttcta
      361 gggttttggc ccttttatgg tttagctcta gaaatgagat ggctaatagg actggaattt
      421 aaaatcacta gaggtcagat aactttagtt tacgagctag cgcttgattg ttagaaatgg
      481 taagaatttt ttaactagag atgcagctga ccaatattac accattggtg gtacaagttt
      541 ggtaggtgaa aaaacgcatg aaactgtttt acgtgaaacc ttagaggaag ttggtattcg
      601 agctaaggtt aatcaactgg cttttatggt tgagaatcat tttgatatcg atgatgtttt
      661 ctggcataat attgaatttc attatttggt aagcccctta gaagacccta aactagaaat
      721 gatagagaat gcaagcgaca ggtttgtgaa tggattccaa tcaatcaact ggatcaaatg
      781 aatcttgttc ctgagttttt acaaaccgag ttggctaaat ggccaggaca tattgtccgc
      841 attgaaggct aaactaaagc gtatgatttt aaatgaatag actgaaataa aggattttaa
      901 aaaaatgaca catgaattta cagaaagcta tgacgtcatt gtcataggtg caggtcatgc
      961 tggtgttgaa gccagtttgg ccactagtcg tatgggttgt aaaactttat tggcaacaat
     1021 caaccttgat atgttggctt ttatgccgtg taatccttct attggaggat ctgccaaagg
     1081 gatagtagta cgagaaatcg atgctcttgg cggcgaaatg ggtaaaaata ttgataaaac
     1141 ctatatccag atgaaaatgc ttaatacagg taagggacca gctgtacgcg ctctccgagc
     1201 tcaagcagat aaatccctgt atgcgcgtga gatgaaacat acggttgaaa aacaggctaa
     1261 tttgactctt cgccaaacga tgattgatga tattctagtt gaagatggac gagttgtagg
     1321 tgttttaaca gccacaggac aaaagtttgc agctaaggca gttgtagtga caacaggaac
     1381 agctctgcgt ggcgaaatta ttttaggaga actcaaatat tcttctgggc ctaataatag
     1441 tttggcatca gtaaccttag cagataattt aaaaaaactc ggcttagaaa ttggcagatt
     1501 caaaacagga acgcctcctc gtgtcaaagc atcttctatt aattacgatc aaacggaaat
     1561 tcagccaggt gatgataaac ctaatcattt ttcatttatg tcaaaagatg ctgattacct
     1621 caaagatcaa attccctgct ggttgactta tactaatcaa actagtcatg atatcattaa
     1681 tcagaacctt tacagagcac cgatgttttc tggcattgtt aaaggtgtag gccctcgtta
     1741 ctgcccatct attgaagata agattgttcg ctttgctgat aaagaacgtc atcaactttt
     1801 cttagagcca gaggggcgtg atacagaaga agtgtatgtt caagggttgt caacgagtct
     1861 tccagaagat gtccaaaaag atttgattca ttcgatcaaa ggacttgaaa aggctgaaat
     1921 gatgcgcact ggttatgcta ttgaatatga tattgtttta cctcaccagc tacgtgccac
     1981 acttgaaacc aagctaattt caggactctt tacggctggg caaactaatg gaacttctgg
     2041 gtatgaagag gctgctggcc aaggacttat tgccggaatc aatgctgctt tgaaagttca
     2101 aggtaagcca gaacttattt tgaaacggag cgatgcttat attggcgtta tgattgacga
     2161 tttggttacc aaaggaacct tggaacctta tcgattgttg acatcgcgag ctgagtatcg
     2221 tttgatttta cgtcatgaca acgctgatat gcgtttgaca gagattggtc gcgacattgg
     2281 gttggttgat gatgagcgtt ggaaggcctt cgaaattaag aaaaaccaat ttgataacga
     2341 attaaaacgt ctgaacagta tcaaattaaa accagtaaaa gcaaccaacg atcgtgtgca
     2401 ggagcttggt tttaaaccat tgacggatgc tatgacagct aaagaattta tgcgtcgccc
     2461 agaaattgat tatgcgactg cagtctcttt tgttggttat gtggctgaag atttagatgc
     2521 taaaatcatt gaacttttag aaacagaaat caagtacgaa ggctatattc gcaaagcttt
     2581 agatcaggtt gctaagatga aacgcatgga agaaaaacgt attcctgcaa atattgattg
     2641 ggatgctatt gattctattg caactgaggc gcgtcagaaa tttaagaaaa ttaatcctga
     2701 aacaattgga caagctagtc gtatttcagg agtcaatcct gcggacatca gtatcttgat
     2761 gatttattta gaaggaaacg gaaaagctca tcgaaaatat taaatggaaa tgaaattgta
     2821 atctcaaagc aagtggaagc tctgtatttc agggcttttt tatggtataa tgtgtgctta
     2881 agaggttata agatgaaaaa atttcgtttt gaaaccattc atttgattat gatgggattg
     2941 attttatttg gactactggc actttgtgtc agtattatgc aatcaaaaat attaatatta
     3001 ttagctattt ttctcgtttt gctatttgtt gttgctcttt tatggtatca aaaagaagct
     3061 tatcaattat cagacttagc tcatattgag ctcttaaatg aacaaacaga agataaccta
     3121 aaaacgctac ttgataatat gcctgttgga gtagttcaat ttgatcaaga gaccaacgct
     3181 gtagaatggt ataatcctta tgcagaatta atttttacaa ctgaagaggg ttttatacaa
     3241 aacagtttga ttcagcaaat tattacggaa aaacgtcgtg aagatatttc tcaaacattt
     3301 gaagtttctg gtaataaata cacttcttat attgatgtat catcaggaat tttttatttt
     3361 tttgatagct ttgtgggaaa caggcaatta gctgatgcta gtatgttaag accggttgtt
     3421 ggcattattt cggttgataa ttatgatgat atcactgatg atttatcaga tgcagatacc
     3481 tcaaaaatta attcatttgt agctaacttc attgatgagt ttatggagtc aaaacggatt
     3541 ttttaccgtc gtgttaatat ggatcgctac tatttcttta cagactttaa aacattaaat
     3601 gatttgatgg ataataaatt ttctgtctta gaagagtttc gtaaagaagc tcaagatgct
     3661 cagcgccctt taacactcag cataggaatt tcttttggtg aagaaaatca tagccagatt
     3721 ggacaggtag ctcttgagaa tcttaatatt gcgcttgttc gtggtggtga tcagattgtc
     3781 attcgtgaaa atgcggatca tacgaatcca atttatttcg ggggagggtc tgtttctaca
     3841 gttaaacgtt caagaacacg tacccgtgct atgatgacag ctatttcaga tcgaatcaag
     3901 atggtggaca atgtttttat tgttggacat aggaaacttg atatggatgc actaggttca
     3961 gctgttggta tgcaattttt cgcaggtaac attattgaaa atagttttgc cgtttacaat
     4021 ccagatgaga tgagtccaga tattgaaagg gctattgagc gattgcaggc tgacggaaag
     4081 actcgtctaa tcagtgtttc tcaagcaatg ggtttagtga ctccaagatc acttttagta
     4141 atggttgatc attctaagat atctttaaca ctttctaaag aattttatga acagtttcaa
     4201 aatgttattg tagttgatca tcacagaaga gatgatgatt ttcctgataa tgctatctta
     4261 acttttattg aaagtggagc aagtagtgct gcggagctcg tcactgagtt aattcaattt
     4321 caaaatgcta aaaaacgttt aaataagatc caagccagtg ttttaatggc aggtattatg
     4381 cttgatacta aaaatttttc tacgcgagtg acaagtcgta cctttgatgt cgctagttac
     4441 ttaagaagta aaggaagtga cagtgttgaa attcaaaata tatccgcaac agattttgaa
     4501 gaatataagc aaataaatga aattatttta caaggagaac gtcttggtga cagtatcatt
     4561 gtagctgcag gagaaaaaaa tcatctttat agcaatgtta ttgctagtaa agctgcagat
     4621 acgatactgt caatggctca cgttgaggct agctttgtat tagttgaaac agcttctcat
     4681 aagattgcta tttcagctag aagtcgtagt aaaatcaatg ttcaacgtgt gatggaaaaa
     4741 ttaggtggag gaggccactt taatcttgct gcctgtcagt taacggatat cagtcttccc
     4801 caagcgaagc acttattatt aaaaactatt aatatgacaa tgaaagaaac aggagaagta
     4861 gaatcatgaa agttattttt ttagcagatg taaaaggaaa aggtaaaaaa ggagaaatca
     4921 aagaggtccc aacaggctat gctcaaaatt tccttattaa gaaaaattta gctaaagaag
     4981 caactagtca aagcatcggt gaattaaaag gaaaacaaaa agctgaagaa aaagctcaag
     5041 ctgaaatatt agcagaagct caagctgtca aggctgtttt ggatgaggac aaaacacgtg
     5101 ttcaatttca agaaaaagtt ggtccagatg gacgtacatt tggttccatc acagctaaaa
     5161 agatttcaga agaattgcaa aagcaatttg gtgtaaaagt tgataaacgt catattgttt
     5221 tagatcatcc tatccgcgcc attggtctaa ttgaagttcc tgtgaaatta cataaggaag
     5281 taacagctga gattaaattg gctatcacag aagcataata taaaaaacat cacaagattt
     5341 ggaggttatg aggttgcctg aagtagctga attacgagtt caaccccaag atttactagc
     5401 ggagcaatct gttcttgggt caatctttat ctcacctgat aagctgattg cagtgagaga
     5461 atttatcagt ccagacgatt tttataagta cgctcataaa attatctttc gggcaatgat
     5521 taccctcagc gatcgtaatg atgccattga tgcaaccact ataagaacaa tcctagatga
     5581 tcaagatgat ctgcaaagta ttggtggctt atcctatatt gttgaactag ttaatagtgt
     5641 cccaactagt gctaatgcag aatattatgc taaaattgta gctgagaaag ctatgttgcg
     5701 tgatattatt gctaggttga cagaatctgt caacctagct tatgatgaaa ttttaaaacc
     5761 agaagaggtt atcgctggag ttgagagagc tttaattgaa ctcaatgaac atagtaatcg
     5821 tagtggattt cgcaaaattt cagatgtgct aaaagttaat tacgaggctt tagaagcacg
     5881 ttctaagcag acttcaaatg ttacaggttt accaactggt tttagagacc ttgacaagat
     5941 tacaacaggt ttacacccag atcaattagt tattttagct gctcggccag cagtggggaa
     6001 gactgccttt gttcttaata ttgcgcaaaa tgtggggact aagcaaaaaa agactgttgc
     6061 tattttttct ttggaaatgg gtgctgaaag tttagtagat cgtatgcttg cagcagaagg
     6121 aatggttgat tcgcacagtt taagaacagg gcaactcaca gatcaggatt ggaataatgt
     6181 aacaattgct cagggagctt tggcagaagc accgatttat attgacgata cgcccgggat
     6241 taaaattact gaaatccgcg caagatcacg gaaattgtct caagaagtgg atggtggttt
     6301 aggtcttatc gtaattgact acttacagtt gattacagga actaaacccg aaaatcgtca
     6361 gcaagaggtt tcagatattt caagacagct taaaatccta gctaaagaat tgaaagtacc
     6421 agttattgcc ctaagtcagc tttctcgtgg cgttgagcaa aggcaagata aacgaccagt
     6481 tttatcagat attcgtgaat caggatctat tgagcaggat gccgatattg tagccttctt
     6541 ataccgggac gattattacc gtaaagaagg tgatgatgct gaagaagctg ttgaagataa
     6601 cacaattgaa gttatcctcg agaaaaatag agctggggcg cgtggaacag tcaaactgat
     6661 gttccaaaaa gaatacaaca aattctcaag tatagcccag tttgaagaaa gataagaaag
     6721 agagacaaaa aatgagcgat gcatttacag atgttgcaaa aatgaaaaaa attaaagagg
     6781 acataagagc ccatgaagga cagttagtag aattaaccct tgagaatgga cggaaacggg
     6841 aaaaaaataa aattggtagg cttattgagg tttacagttc actctttatc attgagtata
     6901 gtgatagctc tgatactcca ggagcaattg ataattctta tgtcgaatca tacacctatt
     6961 cagatatttt gacagaaaaa acattaattc gttatttaga ttgatttttg cctcatagag
     7021 gctttttctt tacaataaat tgatataatt attagcgata gaagattatt ctgcctgtat
     7081 tctttttatt ttgaaaaaat tcctataaaa gataatattt ttaggttaga ttgaggtaaa
     7141 aaagaggtct tcaccatggt taagcaaatg atcttagtat taaggttaaa agattatcgt
     7201 gaaataaagt tttgacacaa gtgtaattaa tagaggaact tgaagtatcc ggtaaaacta
     7261 tcttgaagtg aaaaaccgag tagacctccc ctgactaaat acaatatact agccatttca
     7321 gagcaattaa gagtttccag tgaagtgctt ttgatagaga gctaagaaaa aaaacagtca
     7381 aggtttaaga actgcaaatt agcatttaaa ttattttttg ttaatgacag ttgtctttat
     7441 taccataact ataacttctt ataatatagt cttcttaatt catcagtggt ggctcgtccc
     7501 atatctgacc ttaaaatatt aaactgatca tcagatgata gatagactga ctttacaaaa
     7561 caactttctt atggtaaact gttctttgtg cgaaataaca gcagaaaaat atgaagctcg
     7621 tcaacaggtg gttatctatt caagtaacct ttgctgttta ggcgaaaacc ccctgcacga
     7681 atcaggattt tttaaaccgt tatttcctca aaaaatttag aggaggacat gtaatatgtc
     7741 acgttatact ggtccatcat ggaaacaatc acgtcgcctt ggtttatcac ttacaggcac
     7801 aggtaaagaa ttggcacgtc gtaactacgt tcctggtcaa cacggtccaa acaaccgtag
     7861 caaactctca gaatatggtt tgcagttagc tgagaaacaa aaactacgtt tctcatacgg
     7921 tttaggtgaa aaacaattcc gtaacttgtt cgttcaagct acaaaaatca aagaaggaac
     7981 tcttggtttc aactttatgg ttcttttaga acgtcgtctt gacaacgttg tttaccgtct
     8041 aggtttagca actactcgtc gtcaagcacg tcaattcgtt aaccacggtc acatccttgt
     8101 tgacggtaaa cgcgttgata tcccttcata ccgcgttgat ccaggtcaag tgatttcagt
     8161 tcgcgaaaaa tcaatgaaag tacctgcaat ccttgaagct gttgaagcta ctcttggacg
     8221 tccagctttc gtatcatttg atgctgaaaa acttgaaggt tcattgactc gccttccaga
     8281 acgcgatgaa attaacccag aaatcaatga agcacttgtc gttgaattct ataacaaaat
     8341 gctttaattt taagattaaa cttacaaaaa gcctatttaa taggcttttt ttgtatctca
     8401 taacaaatct attctgatcc cttcatttta ctatggcttc taaaaaagtt atcactaagg
     8461 ctgatgactt gtaaaatgat actttcgtaa gggttactat ttaggtactc gtaagcattt
     8521 tgagaacgaa ttgtgtcatt tcttgaggtg attctttttt acctttagca atccaacttt
     8581 gacagacacc aaaaaaagca tgggctaagt agatactttg gtattctttt tccgtttgag
     8641 ataactcttc ggtgcggaac ttgtcctgta aatcagtagt gatgagtagg cgaactttat
     8701 tgatgataaa ggcctgtatt tctttggtac cgttagcaga caaaagggaa gagagaagct
     8761 gttctctttg gagaaattca aatacttcaa ggaaggcttg ttctttattt tgatactttt
     8821 tctcaaaaat atattctaat ttgtgaaaaa aggtttgctg ataatagtca atcatttcat
     8881 atttatcttt atagtgtgtg taaaaactag agcgactaat tccagctctt ttagataatt
     8941 taactgtggt aatatcgtca aaactttcgg tttttagtaa cataaccatt gcttttaaaa
     9001 ttgcttgctt cgtattttct ttacggttga ccatctacca tctctccttt tgaacacttt
     9061 tagacataat gtccaaaagt gaaatttttt atcttgtaaa tttaaattta aattgtataa
     9121 tatattttat cataattttt gaacaccatg tccattttag gagaaaatat gttagaagaa
     9181 ttgaaaacac ttattaaaaa tccaaaatta atgattacaa tgattggtgt ggccctagtg
     9241 cctgccttat ataatttatc ctttctaggc tcaatgtggg atccttatgg tcgggtcaat
     9301 gaccttccca ttgctgttgt taatcatgat aagcctgcaa agagagctga taagtcattg
     9361 acaattgggg atgatatggt agacaagatg tctaaaagta aagatttaga gtatcatttt
     9421 gtctcagcta agcaagctca agagggactt aaagaaggtg attattatat ggttattact
     9481 ttacccgaag atctttctca acgggcagca accttattaa atcccgaacc ccaaaaatta
     9541 actatccgtt accaaacgag taaaggacat ggaatggtcg ctgctaagat gggggaaaca
     9601 gcgatggcta agctgaaaga gtctgtttcg caaaacatta cgaagactta tacctcagca
     9661 gtttttagca gtatgacaga cctccaatca ggattaaaag aagcctcagc tggaagtcaa
     9721 gcacttgctt caggagcgaa gaccgctcag gcggggagtc aaacactttc gacgaactta
     9781 gcagccttaa cgggtgctag ccaacagttt caacaaagta ctggtcgatt gacatcaggt
     9841 ttgactacct atacagatgg tgtcaaccaa gtcaagaatg ggttaggaac attatcaacg
     9901 gacatcccca attatttgaa cggggtttct aggttatccc agggagcttc tcagcttaat
     9961 cagggtcttt cacagttgac acaagcaaca acactttctg acgagaaagc taagggaatt
    10021 caatccttaa ttgtaggact accagtccta aatcaaggca ttcagcaact aaatacagag
    10081 ctatcaacat tgcaaccccc taaccttaat gctgatgagt taggtaatag cttaggagct
    10141 atcgctcaag ctgccaaaca agtcattgtt gaagagactg ccgctcagaa tgaagaatta
    10201 tcggctctcc aagctactag cgtttaccaa tcattaactg ctgaacaaca aggagagtta
    10261 gctgcggccc tcagtcaatc tgataaaagt cagaccgtat ctgcagccca aactatttta
    10321 agttctgttc aaactttgtc aacaagttta cagtctctct ctcaagaaga tcagtcaaaa
    10381 cagttggagc aacttaagga agctgttgca cagattgcta atcaatccaa tcaagctttg
    10441 ccgggagcaa gttctgcttt aactgaatta tcaacgggat tagcaaaggt aaatggtagc
    10501 ttaaatcaac aagttctacc aggaagtaat caattgacaa caggattagc acaattaaac
    10561 aggtataata ctgccattgg ttctggggta ataaaactct cagaaggtac caatgccttg
    10621 tcatccaagt ccggagaatt actagatggt agccatcaat tatcagaggg tgctactaaa
    10681 ctagctgatg gtagttctca attgagtcag ggtggtcatc aattaacgag cggattgact
    10741 gaattatcaa caggattgtc aaccttaaat ggttccttag ccaaagcctc tcagcagtta
    10801 tcgcttgttt ctgtgactga taaaaatgct aaagctgtcg caaaacctct tgtgttaaat
    10861 gagaaagaca aagatggtgt taagacgaat gggatcggga tggcacctta tatgattgct
    10921 gtttctctaa tggttgtggc cctttcaacc aacgtcattt ttgctaattc tttatctggt
    10981 cgtccggtca aagataaatg ggattgggct aaacaaaaat ttgttattaa tggttttatt
    11041 tcgactatgg gatccattgt tctctactta gctattcaat tattagggtt tgaagcccgt
    11101 tatggtatgg aaaccttagg atttattatg ctaagtggtt ggacgtttat ggctcttgtc
    11161 acagctttgg tcggttggga tgatcgatat ggctcttttg cttctttggt catgttattg
    11221 cttcaggttg gctcttcagg tggctcttac cccattgagt taagtggagc attcttccaa
    11281 aagttacatc ctttcttacc aatgacttat gtggtatctg gtttacgaca aaccatttca
    11341 ttatcaggtc atattggagt agaagtgaaa gtcttaactg gtttcttact ggcatttatg
    11401 gtattagcac tactcattta tcgtcctaag aaaacagtct aaataaaata acctcaaaaa
    11461 aaacagcatc taaggcagtt acactagatg ctgttttttg tcttattttg acaataactt
    11521 gacaatttct gtcaagtttt ttattgtctt gttaagatca tttgatagta atgtctccct
    11581 tattgagatt aaccgttaaa tttccagtag ctgctttatt ctcagccaca taagaaataa
    11641 catctgaagt tgattcctta ccaatcttag ctggtgtcag ctgtgatcca agtgagattt
    11701 taccatcttc ggtttttgtc ttataggcgg tcgtctttgg attggtcaaa gttatgtcaa
    11761 cagcaacatc accaccacta atcgtattag tgcctttaag ggtaagagca tgagcagtga
    11821 atatagcacc attatcattg gtttcctcgt tttcttgctc actagtaaca gtgaaagtca
    11881 cattttcaag gcttgtttct tcggcttcaa ccccaccagg agatagagat tggatggtga
    11941 cagatttaag attagtattt tttgtggtta attgtccatg ccctaagcta atcgtcccat
    12001 tttctaaact agaattcgta aggcttgaca acgaatagtc ggctttcaac tcaagattgg
    12061 ctaacaagct atcagtagcg tcaaatgttc caaaaacctt ccctttcttt attctagtat
    12121 ttagaagtgt cagaccacca ctgaattcaa ggtcttgaat cgttaaatcc gatatagtgg
    12181 cttgaggcat gcttgagcct ttgagctctt taagactggt accttttgga agcgttatgg
    12241 tagtggttcg gtagttgtgg ttgcggtcac ttttatttaa tccaaacccg atcagttcca
    12301 tacctccggt gatgatgaca tctctgggtt tttcagaaat atgaagtgtc tcgttctcta
    12361 ttttaatttc taaaggatca ataaatttag gatgtgtgta ataggtcact gttggtcttt
    12421 gaacgggtcc tgtctctact gtaatagttg aggcagaact gtcaatatca agagcagtta
    12481 tttggtcaaa tgttttgatg acctttttgg gagctgtttg gtgtttaatg tcctgaagtc
    12541 ctccagttgc atagccagcc cctagaagaa tgcctcctag aataatacat accacactgg
    12601 tcataaagca tttgcgtgag atatttttca tgcttgagct cctttcttat tactgatcca
    12661 cttaataaag gcaacaaaag cccgtcctga aaaacgaagg attgctgtgg caataatcca
    12721 aataagaaga ccaccaccaa aacacaacaa ggcgctgccg atgcctatag cttgtgcagg
    12781 aatagatgag ccaatcaagg tcaagctttc aaacagagtc actcctgcga caagaatacc
    12841 tgcaatccca aaaatgtagg cggcaaagat agctgtgagc ccgagcatta ggcaggcaat
    12901 aataaagaga atcaacgcta gcaatacggg taaagcaatc ggagcagcaa agagggacaa
    12961 aatggttaat ccaataattg ttgcccgatt tttgggagtt ttctcgggag ttgccaagtg
    13021 ttttcctaga acatttgaaa tgatttcact ggcagcttcc ttaggatttc ctaaatcgtc
    13081 gataacctgg ctttctttgt caggaccagc ttcatcaaaa tattcaatat aatattccat
    13141 cgcttcatgg tagtcttctc taggtagttt gcgcaagtat ttatctagtt ctgctagata
    13201 ctcagtcctt gtcatgtctt aagctccctt caataatccc gtctaaggca aatttatagc
    13261 tctgccattc tttttttaga aaaatcagct gtgctctccc cgaagaagtg acagcataat
    13321 atttacgctt tcgtccctga tgttcttgac tataagttgt caaaaaacct gccttctgca
    13381 attttttaag aataggatag agggtggatt ctttgatatt agccactagt ttaatggttt
    13441 gactaatatc gtaaccgtaa gaatcgtttt tttcgacaat ggctaaaatc agaaattcaa
    13501 tcaaggtagc cgatactgga aaatacattt taggctcctt tcatatataa aaattttata
    13561 tataattatt ttacatattt agtataagct tagttttttg atatgtcaat attttatata
    13621 taaaaaaata atatatatca aaaaccacta actaggagta ataagttgac caaaaagcta
    13681 ggattggtta tgatgaagat aagattaaca gaaagagaaa ttagggaagg ctatgaagga
    13741 cttattaaaa cagtattagg caaaaccact aggagaggag aagcggtacg ccgtattttt
    13801 acctcttatt cttgtcaatg atgactggca tgtcttatat gaagtcagaa gtcagcatat
    13861 ttcacaaccg ggggaagttt ctttcccagg gggacaagta gaaaatcagg agacacttca
    13921 agaagctgct atccgagaaa cagttgagga attgactgtt gatgcgtccc agatccagct
    13981 atggggtgaa attgattatt tagtacagtc ttctagaact attcattgtt tcgttggtca
    14041 attagtcgtt gatgactggg aaagcataca gcctaacgaa gaagttgaca aggtctttac
    14101 agtaccttta cgtcagctac ttgtcactga tcctgtctat tatcatttag aagccactcc
    14161 tattgagaca acggattttc cttttgaccg tattcgtaat gggaaatact accaatttag
    14221 tcaagagtgc cgtagtattc cgttttatga aaatctagaa gaaaccatat ggggaatgac
    14281 tgcccagttt actaagtgtt taaccgatat tctaaatgat gaatcaattg caaagaaaaa
    14341 ccccttagat attgaaaaat ctaaggggag atagagcact agtttgttgg cgtcaacttt
    14401 ttgtcaacaa cctgtaaagg ttatttgtat ttttcttcta attgactcat ccaaagtccg
    14461 agccaaatac caagagcaaa taacacaaag gtcataagcc aggttaagat acctgcatga
    14521 ctgtaggaaa tggattcggg actactacta tttggaataa gaattaatag cgtgctggat
    14581 aatacaatac caatgataaa gtggtaaact tttgaatgat ggtgctgtaa agcataatcc
    14641 atacttttag aaaaagccaa gatggctagg actcctccga tagcaatagg taagaaagtc
    14701 cctaataaat ccaaagattt gaaccctatt aacataggac catagagtcc taagataagt
    14761 aagagatttg acgggcttag tccagggacc agtactccta aagcaattaa ggcacctgct
    14821 aaaataaagg ttaaaaagtt agctggtaag gtaccgatta agtcatttaa gaagtaaaga
    14881 cctagtcctg aagtaacaaa ggtaaggact agccatagcc agtcaacttt gtctctttga
    14941 gactgcttgg ttgactcctt aatcagacta ggaatagtgc cgacaatagc gccagcaaat
    15001 ccccataaga cgataacttg atagtgctta agcaaaaact caacggggaa agaaaagaga
    15061 gcaataccta aaataccacc aattcctact ggtaagaaaa ataagacatt ttcaataaag
    15121 ttatccctca tatgagctaa aaaggaaatc attcgctcat agattcctaa aatggctgcg
    15181 agaacaccac cggatacacc aggtaaaata aaacctagtg caataatcat gcctttaaaa
    15241 actcttgaaa taaatgaaac cattgttacc tcctgaaaag aatgatttta ttgtatcata
    15301 aatttaaaga attgctgac
//
LOCUS       DAUCWO010000041        14598 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUCWO010000041 DAUCWO010000000
VERSION     DAUCWO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14598
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.041"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..643)
                     /locus_tag="VGY20_001195"
     CDS             complement(<1..643)
                     /locus_tag="VGY20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ4274047.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLT"
     gene            785..1258
                     /locus_tag="VGY20_001196"
     CDS             785..1258
                     /locus_tag="VGY20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4274048.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            complement(1425..2141)
                     /locus_tag="VGY20_001197"
     CDS             complement(1425..2141)
                     /locus_tag="VGY20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ4274049.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     TPSLHVLDKDNPHVPLFTEN"
     gene            complement(2155..3234)
                     /locus_tag="VGY20_001198"
     CDS             complement(2155..3234)
                     /locus_tag="VGY20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ4274050.1"
                     /translation="MSLATDYFSRQTPIVEKLVAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKDLPQLLQQEGIYPAYHMSKNNWVSVVLDDKVTDDQLWGLATQ
                     SRQLVNPNGLSNPNSPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYACQVLETDIPNEGYRKNLNIDKLMRLRKCQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            complement(3307..5040)
                     /locus_tag="VGY20_001199"
     CDS             complement(3307..5040)
                     /locus_tag="VGY20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4274051.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNSEIQDFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNPQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVIRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLVLRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLTRGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLSLPKMH
                     ELMADDTQENE"
     gene            complement(5037..5777)
                     /locus_tag="VGY20_001200"
     CDS             complement(5037..5777)
                     /locus_tag="VGY20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ4274052.1"
                     /translation="MYSLLIVEDEYLIRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIILTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            complement(5865..6971)
                     /gene="msrB"
                     /locus_tag="VGY20_001201"
     CDS             complement(5865..6971)
                     /gene="msrB"
                     /locus_tag="VGY20_001201"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ4274053.1"
                     /translation="MEHKWKLWLFIVGMVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKADPNAPVDQKMIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEEYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            complement(7014..7637)
                     /locus_tag="VGY20_001202"
     CDS             complement(7014..7637)
                     /locus_tag="VGY20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ4274054.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKYIGYAKKSDIKKTLKGIH"
     gene            complement(7650..8360)
                     /gene="ccdA2"
                     /locus_tag="VGY20_001203"
     CDS             complement(7650..8360)
                     /gene="ccdA2"
                     /locus_tag="VGY20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ4274055.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHMLLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     repeat_region   8520..8947
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8586..8617
                     /rpt_unit_seq="atttcaatccactcacccatgaagggtgagac"
     gene            complement(9096..9389)
                     /gene="cas2"
                     /locus_tag="VGY20_001204"
     CDS             complement(9096..9389)
                     /gene="cas2"
                     /locus_tag="VGY20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ4274056.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     gene            complement(9400..10425)
                     /gene="cas1c"
                     /locus_tag="VGY20_001205"
     CDS             complement(9400..10425)
                     /gene="cas1c"
                     /locus_tag="VGY20_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ4274057.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQIKLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILLTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            complement(10422..11096)
                     /gene="cas4"
                     /locus_tag="VGY20_001206"
     CDS             complement(10422..11096)
                     /gene="cas4"
                     /locus_tag="VGY20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ4274058.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            complement(11098..11946)
                     /gene="cas7c"
                     /locus_tag="VGY20_001207"
     CDS             complement(11098..11946)
                     /gene="cas7c"
                     /locus_tag="VGY20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ4274059.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            complement(11951..13846)
                     /gene="cas8c"
                     /locus_tag="VGY20_001208"
     CDS             complement(11951..13846)
                     /gene="cas8c"
                     /locus_tag="VGY20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ4274060.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMVDKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQHNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKALHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTITWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTVHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            complement(13846..14574)
                     /gene="cas5c"
                     /locus_tag="VGY20_001209"
     CDS             complement(13846..14574)
                     /gene="cas5c"
                     /locus_tag="VGY20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ4274061.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DATYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
BASE COUNT         4335 a         3202 c         2564 g         4497 t
ORIGIN      
        1 aagtcaaata cttacctttt atggtaatca attcttgctt cttcaaatcc ctaagcacat
       61 ggctaactgt ttctcttgtt gtcccactta tttgagcaat atctgttgtt gttaattgaa
      121 aaggtaaggt atcactgtct ttacccattt caaccagcaa aatcgccaga gattgaatca
      181 cacgagaact agcacttgat gtaatcaagt ttcggaccct caactcatgt aactcgagca
      241 gcttagacat cttactatat aagtgtttca tttgtagacg attttcaagc gaataatctt
      301 caaataaatc gactggaaag taaaaataag tcacgtctgt catggctacc actgaaaaat
      361 ggtaataatc atctgtaaag aggccaccgt aaggaaaaat ggtgccgtgt ctgatgaaat
      421 ccgtatacat gaaggttcct gattgatcgg actgctccac cttgaaataa ccagaggtca
      481 ctagaaaaag cttatctctt ttatctccct caaaaaaaag gatatggtcc tttttagctt
      541 ttcggaattc catctgccct acaattttgt caaattgttc aatagaaaag tagcgaaaat
      601 cgtttagttt tctaaggtat tgataatctt ctcttcgaat cacacaaatc tcctttttct
      661 aaacatcatc tttattttac cataaaaaag ctattttgta agcggttcat caaaaacttt
      721 acaccttctc ctctttttgc tatactcgga ttgttatcaa ttacgttaaa ggaagattat
      781 aacaatgaat aagaaagaga cgcgtcacca actcatacgt tccctcatct ctgagacaac
      841 tatacataca cagcaagaat tacaagaacg gttgcaaaaa aatggcatca cgatcactca
      901 ggctacccta tcacgtgaca tgaaggaatt gaacctggtc aaagtaactt ctggaaacga
      961 cacacactat gaagcgttag ccatttcaca aacccgctgg gaacatcgtc ttcgttttta
     1021 catggaagac gctttggtca tgttaaagat agttcaacat caaattattt taaaaacgtt
     1081 acctggacta gcccaatcct ttggctccat tttagatgcc atgcaaattc cagaaattgt
     1141 agcaacggtc tgtggtgatg atacttgctt gattgtctgt gaagacaatg agcaagccaa
     1201 ggcttgttac gaaaccctta gtcattacac gccacctttc ttctttagta acaaataagg
     1261 cttaacagaa aacctctatg tgatatacga cctcatcctc accttttgct aggacagcag
     1321 taaaatctca tcaaaaagcg agtaaacgaa aacctaaatg acaggacatt cgtttactcg
     1381 ctttttttgt taaccttcta ttttacacag ctcatcctac taatctagtt ttctgtaaaa
     1441 agtggcacgt gtggattgtc tttatccaac acatgcaaag agggggttgc atgaaggtac
     1501 atttttgcgc tgccttcaat gaagtcaaga gcttcttgca atctattttg accttcttga
     1561 tcaagagctt cgataatcag aaatgcattt ttggtgtgtt ctgtcaccat ggtgcgttcg
     1621 ccatctcgcc agttaaggca acgacaaacc gctccaatat catctttata acacagctca
     1681 tttggcaagg ttgggttgtt atcagcatcc ccaatcaagt aaaagtcgtc accaccatct
     1741 gttatcgtca gacgcaaatc tccgataaaa ctgtcactat cttcggctcc aacaggtaaa
     1801 ccaaaacgta gactagccgc attataaata tccactagcg gattgatcga cggaatgctt
     1861 tgaccatttg acacccgctt gagaagggct tcaatgctag agcgagctcc ttttttggtt
     1921 ttaaagtgtt gataggcctt gcggtagact tgaaccacct gattatcact aaaattgtct
     1981 gctgttaaat aagcttttgc taactcatgg ctatctgcta agagctgttt gacatcttct
     2041 ggtgactctg cttgattctg aacatccttt aataagacga cacctagttt ggcatctgga
     2101 aaaagttccc aaaaactttg atctgctaca aattcaacca tctcttattc tcctttagtt
     2161 gttctcttta aaatactctt tttcctttaa aaatgcaatt aattgagggc ttaatcgcct
     2221 tggccctctc actgcagcta cgccatgttc tttcataagg tcaaaagaca agagaccatc
     2281 cttgtactgt tggcattttc ggagacgcat gagtttgtca atgttaagat ttttacgata
     2341 accctcgtta ggtatatcag tttctagaac ttggcaggca tagcgaatgg atttgactgg
     2401 ggctgttata taaatcagca cgtagtctcc cacggcaata ccagcttttt gggtccataa
     2461 aatcacatca ttggctgcaa attctgcatc aatatcatag tatttcaaat tagctggaat
     2521 gacccaataa tcaggactat taggattaga aagaccgttg gggttgacta gttgacgact
     2581 ttgcgtagct aacccccaaa gctgatcatc tgttacttta tcatccaaga caacactaac
     2641 ccaattgttt tttgacatat ggtaggcagg gtaaatgccc tcttgctgca gtaactgagg
     2701 taaatccttg ggattaactt tgacagtcat cacttcacat gtctgtccta ctaatctctc
     2761 tggtatctgg tctaatttat cagccaagag agaaaaaatc atagcatacc atttgccccc
     2821 tacccggtag gttgcaaggt ctgggtgctt ctcaaaagga tagtccattg ggtcagacca
     2881 ttctttggtg atatgcttag caagtctatt agcttgcatt gactgaaaag gagtgacttc
     2941 aaaacaggct actgataagc gctccaaaag ttccaaatag gctgctctaa cctgcccagt
     3001 ataggttcct tgccaggctg cttgttgtaa aggcaaataa tcctcctcca aatcacaatc
     3061 aataacccga tcccagatat tgccagcctc atcaatccta agctgcgcct caaattctcc
     3121 ttccataaac cgttcgttat agaaataacc attatctcgt ttttcaaaac cgtaagccac
     3181 tagcttttca actatggggg tctgtcggct aaaatagtca gttgctaaac tcatgatatc
     3241 ctttctaagg ttcctatctc caatacccta acttagggaa atggctgaca tgcacttcct
     3301 gtaaccttac tcgttttcct gtgtgtcgtc tgccattaat tcatgcattt taggaaggct
     3361 taattgtatt tcagtgaact ggtctgactg gctatgaatg gtcatgtggt agccttcacc
     3421 atagtgaagt tttaaccgct ggtcaacatt tttaaggccc acacctcccc tagtcaacaa
     3481 gctctgacta ttggtcagtg aagagtcttc aatgccttta ccattatccc aaacagtcaa
     3541 caccagatgc tgagctgtat cagataccgt aaccttgatc atgcctttgc gatcgacttc
     3601 tttgatgcca tggtagatag cattttctac taagggctgt aagataagct taggaataac
     3661 aaagtctgcg tagacatcta agccttgcac ttcataactt agcttgtctc catagcgctg
     3721 tttttgaata aaaaggtatt ggctaacgtg atccagttca tctgccaaac gaatgtattc
     3781 gttcccctga ttaagggcca aacgaaaata cttagctaga gacttggtca cttctaccac
     3841 gcgcttgctg tcattaaatt ctgccatcca aataatagtg tccaaggtgt tatagagaaa
     3901 atgcgggtta atctgactag ccaaggcttg taacctgtac tgcccaatcg ccttttcctt
     3961 atccgcaacg gcaaccatca agctatcaat ttgatctaag agcgcattaa actgttgggc
     4021 aaggtctgtc aattctggag aacccgtttc ttgggcacgt aactgacgat ctcctttttg
     4081 aatagcaaga ataacttgct gcaattgttg aatgggacga atccatctgc gtaagactaa
     4141 gacagttgca aagccacaaa tcagacaggc tagaagccct gttccaagaa aggaccaaaa
     4201 aatttggcgc tgcacccggt gcaactgatc cagtgacgcc actcccacta aagtccattg
     4261 actgttagga atgagttttt ggtaaacaaa agatgtcttg tccttagtgt aaccattctg
     4321 aatcgctaaa taaggcttca tcgcagccat ctccttagaa gaactgtaaa cactcttttt
     4381 gggatggtaa acaaattcat gcttatcatt cacaataaag gcaaagcctt ggcggcctag
     4441 ctgaagctga tctaaagacg ctttaatagt cggatatgca atatcaagtc ttatcacgcc
     4501 taaattatga ccagcagcat ccactacctc ttgggtcaca gaaactaccc attctttttt
     4561 agaagaaagg gataattgcc gagctggggt taagattggc atggcttgac ggtctatggc
     4621 tgctttatac cagggttctg ccatcatatc actggaggtt ttcataatca actgaggatt
     4681 ggtagaaacg gttcggccat ccttagtcac cagaacagct gcttgtaaat cggaattggt
     4741 cgcaagaacc atcttcatga gctgaagaac ctgtttgtca ttttcttgat gaatttgact
     4801 agcaaagtct tgaatttccg aatttttcgc cagcaccgaa gttgtttctt taagaggctt
     4861 gatgtaagcg tcaataaatt gggaactttg ccgaatgctc tgccgggtat cacgttcaat
     4921 taattgcctg atattacgag aactcgtctg gtaataaagc aaaccaagcg tggtaatgag
     4981 ggcaattacg atcacaaaga cataagaaat caactggacc agtaggggat aacgcttcat
     5041 gcctggcctc cttttttata ctgacttggg gtaacgccta ccagttgctt aaagcgctga
     5101 gaaaaataat tcatgtcctc aaaaccgacc tgttctgcaa tttcatagat tttaaggtta
     5161 ctggttaaca agagaagctt ggcttttttc aaccgttctt gtaccagata atcttgaaag
     5221 ggcatcccta actctttttt aatcaagacg ctaaggtaat tagggctaaa accaagctgc
     5281 tgagccaagc tttttagggt caaatcagaa tctgccaacc gctcatgaat agccattgct
     5341 attgctgata cttctttttg aggctgctca accaattcct gaatggtttc tgttttcttg
     5401 gaaagttcta atttttgctg gagctttcct aacatgtctt ctacatctgc cttggaaaag
     5461 ggtttgagca agtaatcatc tgcccctaat ttcaaagcag ataaggcata gttaaaatca
     5521 tcgtagcccg tcaaaaatac cagatgagtt tggggggatt cctgtttaat gagttctgct
     5581 agttgaatcc catttaattt gggcatattg atatccgtca aaataatatc ataaggctct
     5641 ttctgaaaca agtcccaagc caactggcca ttttctgctt cgttgacccg atcaatcttg
     5701 aactggctaa aatcaaccaa agaacgaata ccttggcgga taaggtattc gtcttctaca
     5761 attaacaatg agtacacacg gtgtctcctt atctttataa agggccttgc atggccctct
     5821 catactagtc tttcttctat tatatcagat ttttaggcct aaccctattc tacagatgat
     5881 aataaataac catagccctt agcttccata tcagcttttg gaataaaggt aatagacaag
     5941 ctattgatgc agtaacgaag gccgccttgg tctttaggtc cgtcagtaaa gacatgcccc
     6001 agatgagaat tccctgaacg gctgcgcact tctgttctcg tcatattgaa actcttatct
     6061 tccttgtagc ggacaacgtc tgggctgatg gggcggctaa agcttggcca accacaacct
     6121 gattcaaatt tgtcttttga agagaaaagc ggttctcctg tcacaacgtc tacgtaaatt
     6181 cctgcatcaa aagaatccca gtagcgatta gaaaaagctt tttcagtttc gtttttttgc
     6241 gtcactcggt attcgtctgc agatagtttt gctttgatct cagtggcact tggtttgggg
     6301 tacttgcttt catcaatcac aggataggtc gcttggttga tgtcaatatg gcagtagcca
     6361 tttggatttt tcttgaggta atcttggtgg tactcttcgg ccttaataaa gtgtttcaat
     6421 ggcgcctttt caaccactat ttttttcttg taatccttag ctttttcttt gaagacctca
     6481 tcaataatag ctaaatctgc tttgtcagta tagtaaatcc cagttcggta ttgactaccg
     6541 cgatcatttc cttgcttatt aagactggtt gggtcaataa ttcggaaaaa atgcagtaat
     6601 aattctttca agctaatacg gttcgcatca taggccactt caacggtttc tgcatgacca
     6661 gtctgatgaa ttaactggta attagtggta tcgcctctac cattggcata tcctgacacc
     6721 gcatctaaga cgccatccac ttgtgaaaaa tattcttcaa cgccccagaa acagccacca
     6781 gctaggtaaa tcattttttg atcaacaggg gcattgggat ctgctttagt ggatttgact
     6841 tttttagctg gtagttgagg cttgccttga ctaaaagcct tggcttgacc tttctgaatg
     6901 agaacagaag ctccgcctat taacaagacc attccaacga taaatagcca taatttccat
     6961 ttgtgttcca taaactcaac tccttttttc taatacctat cagctggtcc ttcctaatgt
     7021 atacctttaa gggtcttttt gatgtctgat tttttggcat aaccaatgta ttttttagca
     7081 agaacaccat cacttccaat gaaaacttct gttgggtaag atctgacgcc gtaagcttcc
     7141 aataacttgc catccggatc taacaagact ggtaagtcct tatagtctgt tccttggaac
     7201 cattttttaa agtctgcttc tgatttttcc ccttgatgac ctggcgagac aactgttagg
     7261 accacatagt ctttatctga cattttagcc aggtcttcgg tatctgccaa ggttgataga
     7321 cagattgaac accaagaggc ccaaaatttc aaataaactt ttttaccttt gaactctgat
     7381 aagcgatagg tcttaccatc aatgcctttg agttcgaaat caggcgccat aggaccatca
     7441 ttggtcattt tcttatcaga tgatttatct gccatcatgg acttgtcctt tgatgttgac
     7501 atcttatctt tctttttagc tgccatgctc atcttgtcct gagttatttc ctttttagcc
     7561 atattgtctt gggttgagca agcagttagt agcccgagac aagccaaacc agttgttact
     7621 aatagtcctt ttttcattgt gtttctcctt tatccaaaaa gttgtgctaa gttatttaag
     7681 gttcctgtca taagcaagat tcccatgact ataatcagaa cgcctcctac ttttttcagt
     7741 aaaagcatgt ggggtttgag cttgttaaac tgtttcaaaa caatgcctga cgcaaaagag
     7801 ataagcagga aaggaatgcc caatccaaga gtataaataa tcatcaaaac gccaccttgc
     7861 caggcaccat ttccccctga agccaccaaa gctagaacag aactcaaaac aggtcccaca
     7921 caaggcgtcc aaccaaagct aaaggttaaa ccaaggataa atgcattgaa caaaccgttg
     7981 cgattcttgt tattatggaa agtcagtgat ttttgaaatt gtaagctctt aatggtaatg
     8041 aggcccatct gatgaatccc caagataatg accaaggctc ccagtaagta ccgaaaccat
     8101 accgcataaa ggatatttcc taaaaaacca gccccgtaac ctaaaatcac aaaaatagta
     8161 gatagaccaa aaataaaggc taaagttttg acaatgccat accaatagag ttttttacca
     8221 aatatagtaa tcgtctttga atcatctgca tccagtaaaa tgcctaaata gacaggaagg
     8281 accggaaaga tacacggaga gaaaaaggat aacaagcctg ccccaaaaac cgataccatt
     8341 aatacaagtg atagtgtcat aaataactcc tctcttaagt tacccttagt ttaccgaaag
     8401 ctagtggcgc aaaccataga ctagaacatg caaaaacttg aatttgatac gctatcagat
     8461 aaatggttga ctaaaggcat gtaaaaaccc gacctcatta aaagagagat cgggttcatc
     8521 tttcaatcca ctcacccatg aagggtgaga ctgtacgcga gtatgtgact aaatacgccg
     8581 tagcaatttc aatccactca cccatgaagg gtgagactcg ttatccttgt gttcattttg
     8641 cgtgtaatca tatttcaatc cactcaccca tgaagggtga gacttgccca tgccccactt
     8701 gcagagccgt tattagcaat ttcaatccac tcacccatga agggtgagac tttaagcatg
     8761 gattgctggt taggtcccat aactatttca atccactcac ccatgaaggg tgagacaaat
     8821 caaaggatga tgttgctgac aaattggcta tttcaatcca ctcacccatg aagggtgaga
     8881 cgaccattta aaaactaaag ttcctcctac ttttaatttc aatccactca cccatgaagg
     8941 gtgagactgc gccaaatgac acagtttata gagaaacgga tggcacacat agaggtgttt
     9001 taagcctttt tatctccatt tcgcttcttt tttaagttat ttttgcgcga gcaagcaagg
     9061 tttttctgtg aaagctagct tcgcacaaga gatttttaca ataataagac acctttatct
     9121 gggtcatagc tgtctgagcg accaagtgtt tccacacgcc tctgccaatt tttccccaat
     9181 aaataaaagc gaatactatc agttttctca tcaatgattt gtgttaagcg gtgctttata
     9241 tccacaaatt ctgcgggtgt cacagaacat tcaaaaacag aattttgaac acgttgccca
     9301 tagtccacac agagtttggc aacatgacgc aatctttttc taccagcagg tgtttccgta
     9361 tttacatcat aagtgactaa aaccatcata taacatctcc taaaccataa aaggtggata
     9421 gctttctaaa tctcctcgta tggccttagc taaaagctgc gcttgtacat agggtaataa
     9481 cataagtttt actttctctt ttgtaaaagg atgttctacc tcagtatgct tacgcttctg
     9541 ccacaaatca ataaaaatag ctctgccatt ttccgtcaat aaaatactac cattttcttt
     9601 aacctcaaag tgttttttct gaagttgtcc tttattaatt aatgaaaaga caaaacgatc
     9661 tacaatatat gagcggaact cttcaactaa atcaagcgct aaactagcac gcccaggacg
     9721 atccgtgtga aagaaaccaa cgtaactgtc taatccgaca gcttccaagg cagattgaca
     9781 ttcaaaggtc agtaaactgt aaccaaaaga caagagggca ttaacacaat ctaagggtgg
     9841 tcgtttactc cgaccttgga agtaaaacgt ttttttgtcc gtcaacacca ggtcattaaa
     9901 tatgcgaaaa tactgattag cagcttggcc ttcaatccct cttaaagagt ctttattatc
     9961 tgctgcctga accatctcta aagcccatat caattcgtca ttaacagcct caaaaagctt
    10021 ggtatcaatc tgttgacgat gatctcgttt aaagcgtagc aagtatttcc ttgagttgga
    10081 aattttagcc aaaataaacc gctttgcgta ttccaaagat tcctcacgat cagataaacg
    10141 ataatgttct ctacgcaaca acacattccc attggttgaa ccaatatagc gaccacaaaa
    10201 acgcccttgt ggtgtatgaa acgataattt aatctgattc tccgtacata acttcaccaa
    10261 agcaggcgac acacccaaat aagaaaaaca gacaataccg tcaataatcc gaaacggaaa
    10321 ccgtttgaga accttacctt cttgcttgat aacaatgtta tcgccctctt tagtgacata
    10381 aaaatcttct tgcgtcaaat acaaggtatt tagcaacttc ttcataggtc catctcctca
    10441 ctggtcatag cctcattgat gtaacgcgac acactccttg tttttttact caacctgggc
    10501 ttacaaatat cgactaaaga acaaagctga cagtttttaa aataagctgc tttaggtagc
    10561 atttgactct gataaacctc atgcatctct gcggctaatt ccttcacttc ttgacgcaaa
    10621 gctgatgtca taggaacaat cactctttga ttgacactat ggtaataaag acaaccttcg
    10681 ttaatgtcgc agtctagcgt ttcttctaaa cacatggtct gagccaccaa ctggacaata
    10741 tctctggtat cttttttagg ttttccgcgc ttgtattcca caacttttgg taaccatttc
    10801 ccacgttttc ccctcaaaga cacaccttga tcatctttat aaaattccac aacatccata
    10861 attcctgaaa gtccaagttc tgcagaagaa atgggcatag cacgtgagac caaaagctct
    10921 tttcgttttt ctttaatgta agggttatct gctttagtat gtaaaacctg tccatgcgct
    10981 gtcgcttcat tatcaagcca ttgttgctca atatggatca acgcccattg acgtttacaa
    11041 aattggaaat gctgaatacc tgatagcatt aaataatcat cttcggcata gaccatacta
    11101 gagtccttct aggatttcaa ccgttaaccc tttcgcttca tatttagcca atttttcttg
    11161 atttagatga atctgataag cgtcataagt gcttttttct tctattgatt gattatactc
    11221 taacaagtca aagacacgcg cacttgaaac atttcccaat ttgcttgaat gcgtaaacca
    11281 aaagacttca caaactcgca tagagccttc cggacgtgca gacgacgcat cattttcaaa
    11341 caagctaacc aaaacttctt taatagcctc agcatcttcc tgagaaaaac cagtcttttc
    11401 agcaaaataa gcattgatag aacctttaag tacatacaca ccataatcta caaaatgttt
    11461 tgtccccatc gtatcagaag agcggccact attattctta gcttccatac cattggtact
    11521 acgcgtaatt tgaaggctgg aaatgacaat tggctccaag gacttagcca tactgatgga
    11581 aactggtcca cgcaccccaa ttgatttttt cagataagta aaaacttgtc caaaagcacg
    11641 aacatcaaac cataatgcat ttgctttttc ttcaatttct ttgtcaggtg tcttagctgt
    11701 aaaatgttgc gaaaagcgtt tttccagtga acgaaaatca tcttcaatac gctcattagc
    11761 ttgcacaaaa atagacttcc ccatatcttg caaacgatta cgaatcttac gtttaatgga
    11821 gacatcactc atcacaccat atcctttggc atctgtacga ggcatgtttc catttaaggg
    11881 atcaccattt gcatttgctt ctttcacttc aagagttacc ataaaatcaa ttttgtgttc
    11941 caacatcttt ttactcctca ctttctgtta cttccgtgtt ttgttttgta tagtaatagt
    12001 ttttctcagc ataataccca aaaataaagc ggtaatctaa gggtttttcc attgtttctg
    12061 tttctaacag aggatttaac agttctaaaa tctcttcttt ttctttttct aatttcatcc
    12121 aactgccacg acgatttaat ttcaatggtt cttcgtaagg cttaatttta ttttccaata
    12181 acatcatcaa ttttgttggt tgtccagtat aggctgtcca ataacgttca gcattggtaa
    12241 tacggtcgtt attgtttcca tccaagccat aacgcaaggt ctcgattaat tcaaaaattg
    12301 ctaataatcg tccaaaaaga taggaacgat tttgattggt atgatctagc atcggtgaaa
    12361 attcctcccc attttgtttg tgtaaaattg ctaagcagac ctgctcaact tgataccagt
    12421 gcttacggta acgatgtcgt tctttaacat tgttacctaa ctttttgaca atggactgtg
    12481 gcatcggttt accatcaatc aaacttgcca ctaacttttg aatttgatca cttttgaagt
    12541 tatcattatc taattcaagg aagcgatccc tatcaacccc gtaggacaca ttaaggatgt
    12601 catgaacagt aggggttctt aagcgacttt tcccaaactt agatcgcgac tcccaactgt
    12661 atgtttcctg ccacttgtta agattggtga gtaattggga cgcttgaagc tgacgaaaat
    12721 attttaaagc aatccgccca ttgctggttt tattcaaaat agcaacgtag taagtggcat
    12781 cattagcaaa taatttttgt cccttgatga atgatttacc aattctttta ttgtcttcag
    12841 ttgctaatgt tataacagga ggcgtatcgt catcatcatc ttcttcaagt ccgtcatcaa
    12901 agattggtga tacgatatcc aaccgactat catttgttaa atcatcacta aaccagttga
    12961 ttaaatattg agaagaccct aaccaagtaa tggtattttt attttctaaa aggtacttga
    13021 gcattaaatg aatcttttcg gaagtttcat agccaacact aaaaacgtct tcgcgttctc
    13081 taaaacgtcc tttataagct tctcttttat tactaacaga gatgatttta gcattcccca
    13141 tcaaacctct atgcttatcg gtaagctgtt cctctcgtcc actaatatta caaataccga
    13201 gattttcacg attggcttca acaaaagaaa tataggattg gtgtaaagct ttaaacgtcg
    13261 aaaccgattc atttttaaag ccctggaact gatcaattga aaattctaaa aagcaagctg
    13321 ataaatcaat taatttttct tttccagtgg catcacaaaa tgtgactttt aattgattat
    13381 gttgataatc aggaccaatt aaagaatcga gaatcagtgt cagaaattct ggtttcaaaa
    13441 taaattgctg aacaatggtt aaaaacctct tgacgtcacc ctcctcacaa taatcaatcc
    13501 agttattcag ttgcttatga aaagaatcat actgggactg ccccatttca gcactataat
    13561 aagcaaattt atcgactaga ggatgcggtg caggatgact acctgaccta gcaacagaat
    13621 cagccgttac aggaaaaata atcatttgct tatctaccat aaattctgcc ttgtgaaact
    13681 ggccatcttt gtcaagtttc actgaaatga tatttttacc atttgacttt aaactcgtat
    13741 gataaatcgg cagtaaaacc ggctcattat ttcttttttg atgatcaacc aagtctgcta
    13801 gctctgcttt ttcataagtc ttcaagagag aagtaaaaaa atccatcagt tttctccttt
    13861 ctccatggca tcatactcca tgcattcatc gttaaccgat tttatctcct ctggtgcttt
    13921 aaaagcataa ctagaaagcg tgttaacaat atcgcattca gactgtgctt taaacgtaat
    13981 gactccattt ttcatcacag tctttgtaaa gtatgatttt aatggtgtct ttttgtcctt
    14041 cggataggca aaggaatgga acatgattcc caagtcgata ttgacaccat tataatacga
    14101 cacagtagtc tcatactctt cttggctgat atcatctaca agccctaaac attctcttgt
    14161 acccaaaaac acatctcgac gtcccccttt acgaatagaa cgctccataa tggcttcatg
    14221 tttagctgga agtctatctg agttcaaatc ttttctatct tcattccaaa caaaatgaaa
    14281 cttgatcaga taaacaacat cactcaaata ggatacataa cttaaatctg cactataatc
    14341 atgtaacaga gccctgacac cctgtaattc ggtttgaatc tggttaataa ccttaacctc
    14401 tgtgacgata ttagtaaagg tcggcttata ataggtggca tcaacgatac cattcagtgc
    14461 ctgtctagtc ggaaccgaat aggatgaccg ttccgatccc ccttttgtgg ctggatttgt
    14521 aaaaagagct cgctgaccac ttactctcac gtagaagtct ctagatctgt acaagctact
    14581 ttcctccttc tgtttttt
//
LOCUS       DAUCWO010000042        14291 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUCWO010000042 DAUCWO010000000
VERSION     DAUCWO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.042"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            40..1581
                     /locus_tag="VGY20_001210"
     CDS             40..1581
                     /locus_tag="VGY20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4274062.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSKFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            1562..2227
                     /locus_tag="VGY20_001211"
     CDS             1562..2227
                     /locus_tag="VGY20_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ4274063.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(2282..3355)
                     /locus_tag="VGY20_001212"
     CDS             complement(2282..3355)
                     /locus_tag="VGY20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4274064.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDGIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(3348..4124)
                     /locus_tag="VGY20_001213"
     CDS             complement(3348..4124)
                     /locus_tag="VGY20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274065.1"
                     /translation="MKKFANLYLTSVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(4121..4915)
                     /locus_tag="VGY20_001214"
     CDS             complement(4121..4915)
                     /locus_tag="VGY20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274066.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMIAIMWLTKEKSK"
     gene            complement(4899..6053)
                     /locus_tag="VGY20_001215"
     CDS             complement(4899..6053)
                     /locus_tag="VGY20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274067.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(6099..6986)
                     /gene="murB"
                     /locus_tag="VGY20_001216"
     CDS             complement(6099..6986)
                     /gene="murB"
                     /locus_tag="VGY20_001216"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ4274068.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            complement(7136..7636)
                     /gene="folK"
                     /locus_tag="VGY20_001217"
     CDS             complement(7136..7636)
                     /gene="folK"
                     /locus_tag="VGY20_001217"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ4274069.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            complement(7633..7992)
                     /gene="folB"
                     /locus_tag="VGY20_001218"
     CDS             complement(7633..7992)
                     /gene="folB"
                     /locus_tag="VGY20_001218"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ4274070.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            complement(8008..8799)
                     /gene="folP"
                     /locus_tag="VGY20_001219"
     CDS             complement(8008..8799)
                     /gene="folP"
                     /locus_tag="VGY20_001219"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ4274071.1"
                     /translation="MKIGRFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCRFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLS"
     gene            complement(8808..9392)
                     /gene="folE"
                     /locus_tag="VGY20_001220"
     CDS             complement(8808..9392)
                     /gene="folE"
                     /locus_tag="VGY20_001220"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ4274072.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            complement(9427..10704)
                     /locus_tag="VGY20_001221"
     CDS             complement(9427..10704)
                     /locus_tag="VGY20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ4274073.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRRYWKKHIEKNVLLTH"
     gene            11035..11997
                     /locus_tag="VGY20_001222"
     CDS             11035..11997
                     /locus_tag="VGY20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ4274074.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(12114..13349)
                     /locus_tag="VGY20_001223"
     CDS             complement(12114..13349)
                     /locus_tag="VGY20_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4274075.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(13507..14291)
                     /locus_tag="VGY20_001224"
                     /pseudo
     CDS             complement(13507..14291)
                     /locus_tag="VGY20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         4490 a         3089 c         2422 g         4290 t
ORIGIN      
        1 catttcttat cgtgttatac tcaataaaga ggagacctta tgaaaaaacc actacgttta
       61 tgggccagtt tatcgttgat tttggtatct atgattgttg tcacaacctc tctattttac
      121 gggattatgc tacatgatac gcaccaatcc atcaaaaatc aggaaaccca tttgctaacg
      181 tcaactggaa aaatgttagc aagtcatcaa gcaattaaag aattactcct aaacaaccaa
      241 ccaaatgcta aaacaacagc ctataccaac tccattgcct ccatctataa ccttgattat
      301 gtggtcgtga tgaacatgaa agggattcga ctcacccatc ctaacccgaa aaatatcgga
      361 aaaccttttc aaggaggtga tgaggaggct gttctagcag gaaaaaaggt tatctctacc
      421 gccaagggca ctttaggaaa gtcacttcgc tatctagtac ctgtgtttga cggtgacaaa
      481 cagattggtg ctattgcggt tggcattaaa ctaactaccc taaatgatgt ggctttaacg
      541 tcaaaacgca attataccct gtctttactc ctttgtcttc ttattagttt actggtcacc
      601 agttttattt cttttcgact caagcgtcaa ctgcaccagt tagaaccaag tgaaatttac
      661 cagctctttg aagagcgtaa tgccatgttg gatcaaatcg aagccgccgt atttgtggta
      721 gataaagctg gtatcttaca gctctgcaat caggctggac aaaaattaat tgctcggaaa
      781 tgccagttgg gaaaacctac tggcaacagt ttcaatcacc tctttcctga ttttccaaaa
      841 cttagtttgc aagaggggca tgaacaactc tttcggtatg gtgaggaaga ctacttgtta
      901 gccatttcac ccatctgtgt caaaaacgat caccgaggcc acattatttt catgcgagaa
      961 gcagttaagg ctattgatac cttggatcag ctagcctata ccaccgccta tgcttctgct
     1021 ctacaagccc aaactcataa attcatgaat caattgcatg ttatttatgg cttagtggat
     1081 attgcttatt atgaccaatt gaaaatttat ttagacagta ttttggagcc cgaaaatgaa
     1141 attctcacta gcttatctgt ccttgttaag gagcctcttc tagctagttt tctcattgga
     1201 gagcaagaga aataccaaga attaaatgtc catctcaaga ttgatgtttt aagcgagatt
     1261 cctcattctg ccaccaagaa tcaacttaat aatggtttga tgatttaccg ctttatccat
     1321 actaatcttc taaccacact gagacccaaa tcactcgttt tgagtattca acatgatcag
     1381 aatcatttaa tcagccatta caccttgaca gacaattgga ttgatttgga gcgggtacag
     1441 cctatttttg acttacctta ttttcaacag ctcttgaccg atacaaattc caaattccat
     1501 caacatcaaa ccgtaaccga gttacagttc tcagtgacaa caccttatgt aggagggcaa
     1561 catgaacgtt ttaatcattg aagatgatcc catggtggat tttatccatc gcaattacct
     1621 cgaaaaacta aatctttttg atcgcattat atccagcgac tctatgaaag ctgttcaaag
     1681 catacttact gattacgcaa ttgacctcat tttactggat attcatatta cagatggcaa
     1741 tgggattcaa tttttggaga aatggcgtgc tcagcatatt ccttgcgaag tcattattat
     1801 ttcagccgca aatgatggga atattatccg agatggtttt catttaggaa ttatcgacta
     1861 cctcatcaag ccctttacct ttgaacgctt tcaagaaagc atccaacagt ttgttacaca
     1921 tcgagaacat ttggccaatc agcaactaga gcaggcccaa attgaccaac tgaaatgttt
     1981 gactagcaaa aaagacacta aaaacaaaca attgttagaa aaaggcttat ctgaatccac
     2041 tttccagtgg attatggaaa atatcaaagc ctttgatcaa ccttttacta tccaagaatt
     2101 agcttccgct tgtcacttat cccatgtctc tgtccgtaaa tacattgctt atttagaaga
     2161 aaataagcaa ctgaacagcc agcaaatttt taccaaggtt ggccgtccct atcgcgtgta
     2221 ctggtgacgt catctgtcga tacattaaaa aggctggaga aataccagcc tttttgagtg
     2281 cttatttgcg atacatttta aattggaggt ataaatcatt ataaatcccc aaccatcttg
     2341 accctaaatt atcataaact tccaattttt tgataatgcc atctgttgga taaaaagcag
     2401 gatcattttt tatctcatct ggaagtaagg ccttggcttt tttatttggt gtcgcataac
     2461 caatatatgc agcattttgc gcagcatttt caggacgatt gataaagttc aaaaaagcat
     2521 acgcttcttt ttcatgtttc atggtttttg gtagtaccaa attatcaaac caaaggttag
     2581 acccttctga aggcacgatg tagtgaaggt gttcgttact atctaacatc tcactggctt
     2641 caccagaaaa ggtaattcca atagcagcgt caccttgaat catgtagcct ttcatctcat
     2701 ctgctacaat ggctttaaca ttcggcgtca actgctgcag ttttctctcg gctgcctgca
     2761 actgctctag atttttagaa ttcacactat aaccaaaagt tgttaaacca acccctagca
     2821 tttcacgcgc tccatcaatc agcataatac tatttttata ttctggacgc cacagatctt
     2881 cccagtgcat aggcgcctta tcaactaatt gatcattata aacaatccca acggttcccc
     2941 agaaataagg caaagaatag tcgttttgtg ggtcaaagct tttccctaaa aattctttcc
     3001 cgatattatc catgccaact aattttgact tatcaagctt attgagtagg ttttctttga
     3061 tcattttatc aatggtgtaa tcactaggaa cagcaatgtc gtaagtggtt ccgccttgct
     3121 tgattttagt gtacatggct tcattggaat cgaaagtttc atactgcact tcaatgcccg
     3181 tttctttggt gaattttttg agcaaagctg gatcaatgta atctccccag ttataaataa
     3241 ctaatttatc cgattgacta ccagaacctg attttttctg caagatgaaa gaaagacttg
     3301 ttaaaataac aataacaccc aaaactcctg ctagaaagga ataaagttta cgcatgtttt
     3361 tcctccttgt cctgtgacat ataataataa ccgatcacta agaggatgga gaaaaagaaa
     3421 acgatggttg acaaagcatt gatatccaag gaaattccct gacgagcccg cgaataaatc
     3481 tcaacagata aagtagtaaa accatttcca gtcaaaaaga aagtcactgc aaaatcatct
     3541 aaggaatagg taaaggccat aaaataacct gcaataatcc ctggtgttaa gtatggcagc
     3601 atgacttctt tgagcatttg gaaataatta gctcccaaat cataagcggc gttgaccatg
     3661 tcttgattca tctctttcaa gcgcggcaat accatcaaaa ccacaatagg aatcgaaaaa
     3721 gcaatatgac ttaataaaac tgaagacatg cccagctgaa acttcaatga tgtaaaaaga
     3781 attaaaaagg aagccccaat catgacatct ggtgatacca tcaagacatt attggctgat
     3841 agcatggcat tttggtactt acctctaaca tggtggataa agatagctcc aaaggtccca
     3901 ataatcgttg ctagtagagc gctactaaaa gcaagaacaa aggtttgcag taagattgtc
     3961 atgagacgac tatcctcaaa catggtttgg taatgctcaa gggtaaatcc tgtaaaacca
     4021 ttcatatccc cacctttgtt gaaagaatag aaaatcaaat aaaaaatagg aatgtagagt
     4081 aaaacaaaga cactcgttaa ataaagattg gcaaattttt tcatttactt ttctcctttg
     4141 tgagccacat aatagcaatc atggttaaaa ttaacaccac acctatggtt gatcccattc
     4201 cccagttttg ggtggtcaaa aaatgttgtt caatggctgt accaagtgta atcacccggt
     4261 ttccaccaat caaacgggtt aacataaaga gactcaaact tgggataaag acagactgaa
     4321 cacctgccct aaccccattt aaagaaaggg gaaaaataac ttttgagaag gcctgaaatt
     4381 cactagctcc tagatcgcga ctggcattga tgacattatg gtcaatatca tccaaagcat
     4441 taaaaatcgg taacatcata aaaggaagct caatgtaaga ggctacaaaa atgaaggaga
     4501 aatccgtgaa aaggatttgc tgcgggccaa tccccataaa ggttaaaaag ctgttaatcc
     4561 ctccttgttg accaaagatt cccataaagg cataggcttt tagcaataag tttacccaag
     4621 ttggcaaaat aatgagcata agccacaact gcttgtgctt taggcgcgtt aaaaagagag
     4681 ccgtaggata tgaaattaag agcgtgacga gtgtgataat accagcgtat aaaatagaat
     4741 tcacactcat tctcaaatag gtccaagagc taaaaaaggt ttcataattg gctaaggtca
     4801 cgcgcccttc tatgtcaaaa aaggacttgt aaaacaagag agtgactggt gccacaacaa
     4861 agaagagaat ccataagaag taaggaatcg aaaaaagact agaggttttc ttcattacgc
     4921 tcctcctcaa tggcattaat caatccatct tcaggctcat ccatttccac atattcttca
     4981 atacgggcat caaattcctc ttcagtctca ttaagacgca tgatatgaag atcttcaggg
     5041 gtaaaatcta atccgataac ttctccttcg atagctttgc gggtagaatg aatcatccat
     5101 tcattaccca attcatcata ggcaataatt tcgtagtgaa ccccgcggaa taattgggta
     5161 tcaaccttaa cttgtaattt cccttcttct ggcaaagtaa tttgaagatc ttcaggacga
     5221 ataaccactt caacaggctc attagggcgc attccaccgt ccacagattc aaattctttc
     5281 ccgttaaagg agacaagata gtcttcaatc atggtaccgt taataatatt agattctcca
     5341 ataaaattag caacaaaatg attaattggc tcatcgtaaa tatccactgg tgttcccgac
     5401 tgaacaattt caccttcatt catgacaaaa atccaatcac tcatggccaa ggcttcttct
     5461 tgatcgtgag taacaaaaac aaaggtaata cctagacgtt gttgcaattc acgtaattca
     5521 tattgcatct ctgttcttaa tttcaaatca agggctgaga gtggttcgtc aagtaagacc
     5581 acacgcggtt gattgataat cgcacgcgca atggcaacac gttgacgttg accaccagat
     5641 aacttctgga tagaacgatt ttcataccct tctaattgaa ccattttcaa ggtttctttc
     5701 acgcgcttag caatctcttt cttgtccact ttcttcagct tcaaagcaaa ggcaacattc
     5761 tcaaaaacag tcatatgagg gaagagggca taattttgga aaacggtatg aatgtcacgt
     5821 ttattaatcg gcaaatcatt aatacgttcc ccatctaaat aaatgtctcc gctgctggca
     5881 tctagtaggc ccgccataat atttaaaata gttgattttc cagagccaga agctccaagc
     5941 agggtgtaaa attttccttc ttcaaggtca aagttaatgt tctttagaac ctgtgttcca
     6001 ctgtcctcaa atgtttttga aacattgtta aatgtgataa ttggcttagt caattgtcat
     6061 aaatcctcca cggtactttc tttaattatc aataatggtt acaggttttc cccaataatc
     6121 cgaacttctg gttcaagcct gacaccagaa tggttttcaa ctgtctcaat gacataagca
     6181 atcaaatctt cataatcttt agctgtgcca tctgccacat taatcataaa accagcgtgt
     6241 ttttcggaaa cttccacacc accaatccga tgccctttaa gatttgcttc catgattaat
     6301 tgacctgcaa agtgtcctgg cgggcgctta aacacagatc cacaagatgg aaattctaaa
     6361 ggttgtttta gttggcgaag gtgatttaac cgattcattt cttggctgat cgtatcataa
     6421 ttacccggtt taagagcaaa cttagcagaa atgacaatgt cacctgtttc ttgaatggca
     6481 gagtgacggt aaccaaaagc catatcccta gctgaaatgg ttttgatctc tccacttgag
     6541 gtaagcacct tagcggataa gaaaatatgt gatatttccc caccataggc acctgcattc
     6601 ataaaaacag cacccccaat gcttccagga atgccacatg caaattcaaa ccctgttaaa
     6661 ctatggaatt tggcaatctt cgttgtttca atcagattgg caccagcttc ggcttctaag
     6721 gtataaccat ttaagtgcac tgcatttagc ttatcaaaca tgatgacaaa gccacgaata
     6781 ccaccatctc gcacaatgag attgctggca tttcccaaaa caagccaagg catattttct
     6841 ttgttggcat aagcaacgat gcgtgatagc tcgtagtgat ttctaggaaa agccaagaaa
     6901 tctgctgggc cacctacctt agtgtaagtg taatgtttta ggggttcatt ctctcgaata
     6961 tcaatcccat gaagttcagt tatcatagtt atcttctttc ttttatcact tcttatacgc
     7021 gtaaaaaaat cggcataaag ccgatactat tatatcaaat tttagaaaaa ataacgatga
     7081 atttggcaaa actttatgga ttggcttata gcaggcatta ctccgaaaac agagtctaga
     7141 actctgtctg ttgagctgaa aagagcgtga tatctgattg atctaaagct gcaagataat
     7201 cccttaaaaa cttatgatta ggaggcaatt ttaaatctgg ctgcaattcc aataagggga
     7261 ttaaaacaaa agcacgctca gtcatataag ggtgtggcac tttcaattcc tttgtgtcat
     7321 aaacctcttc tccaaatagc aagatatcaa tgtcaatagt tcttgatccc catttttcat
     7381 gcctcacacg accaagagat tgctcaatag cttgtgtttc ttttaagaaa tcagccgcgg
     7441 ttaattgtgt atctaattga caggccatat tgagaaaatc agcctggcct gtcttgcccc
     7501 aagcagtcgt ttcataaatg gatgattgag caagcaaccg tgtttgaggt aagtcagcta
     7561 aagcctcaag agctttttgc aagtaagctg ctcggtcccc catattggtg cctaaactta
     7621 aataaacaat ggtcatctct gtctctccaa ttcaatacca actgccttat agtggcctgc
     7681 aatcggtggg ttttcctttt taatggccac tttgatggct tcaataggtg gaaattcgtt
     7741 aaaaagctgt tcacaaatcg cacctgctaa acgttcaatc aaaataaact ttcccccttc
     7801 cactaactga cggacactat caaacaccat cccgtaatgg accgtatctg ttaattggtc
     7861 tgacaaagaa gctgcttgca aatcgactga caattctaaa tcaaccagaa aaatttgccc
     7921 aagggtctgt tcttctttaa aggctccatg gtagccataa aaacggcaac cttctaatac
     7981 gattttatcc ataagtcctc acatcaacta gctcaaaaca gccacaatgt cttgattagc
     8041 cttgacatcg tgtacgcgta caatctgaca gccttttcca agggcataag cagacaaggc
     8101 tgctgtcgct ccgtctcgct ctttagcttt ggtgttgccg cctaacaagg catctacgac
     8161 acgctttctc gaaataccaa acaagacagg atagcccaac tgacagacgc ggtccaatcc
     8221 ttttaataac tccatattct gttgaacaga tttggcaaat ccaaatcctg gatcaatcca
     8281 aatattgttt tttggcacgc cagcatcaag agctgcttgt gctctattgc ctagaaaatc
     8341 acaaacgtct tgtgttacct cttgataaac ttcttcgtct tggttatgca tcaagatgat
     8401 aggcgcatcg tactcggctg ctaaggcaaa catttgtccg tcgtacaaac ctgcccaaac
     8461 atcattgaga atatcggcac ccgcctctaa agctgctcta gctgtttcgg ttttataggt
     8521 atcaatgctg attagaatat catagttttc tttgatggcc ttgatcacag gaaccacccg
     8581 gtcaatttca tcggtagcgc ttacaaaacg gcaacctgga cgtgttgatt ctccaccaac
     8641 gtcgatgatt ttagcaccat cagcaatcat ttgctcaacg tgatctaatg ctttttgcac
     8701 agtagtgtaa gaccccccat ctgaaaaaga atctggagtc acatttaaaa tccccatgat
     8761 agccgcattg ccctcaatca caaaccttcc aattttcata agtcctccta atcttttgtc
     8821 attagagaaa taacttcttg tctctcagca cgactttcct tgtataagcc tctggcagtg
     8881 gtggtaatgg ttttactacc tggctttttg atgcctcgca tggtcatgca catgtgctct
     8941 gcttctacca taactaaagt ccctttagga ttgagggctt caactaaagc atccgctatt
     9001 tgagaggtca aacgctcttg gagttgaggt cgtttactag ctacttcaac tgctcgcgct
     9061 aatttactca aacctgttac tcgaccatca cttggtaagt aagcgatatg agccttgcca
     9121 taaaaaggaa ctaagtggtg ttcacatata gaataaaaac taatgtcttt gacaatcacc
     9181 acatcctcat gttgttcttt aaagacagcc gtaaactcct ctttaggatc ttttcccagt
     9241 cccaaaaaca tctccgcata cattttagca acccgtttag gcgtgtcaag aagaccttcc
     9301 cgatttggat tttcaccaat agcctctaaa aactgataaa tggctgcttc tgctttttcc
     9361 ttattaatag acattagtct ttcccttttc attagttttt tccttttcct tttcattttc
     9421 tcttttttaa tgggttaaaa gaacgttttt ttcaatatgt tttttccaat accgtctaat
     9481 ttctgaaata aaatatagtg accctgtaat cacgaaaaag tcatctgctt tagcatgctt
     9541 acgcagggcc aagaaatctt tgaaatcagc aaccctacca aaacgttctg ggtatttttc
     9601 caatggatag gcgttagggt aatgaaagcc agtaacctgc aagtcaccta tttgctcaag
     9661 agcaaccaac atatccgcaa taggcttggt gtcgatcgca ccaaacaaaa tatgaagctt
     9721 tttatcattg tagttatttt ttaagacggc taccaaagca gcaacgcttt cattgttatg
     9781 agccccatca atcattagat ttggtgctaa taattcggta cggcctagcc acaaactatt
     9841 tgccagacct tcccttatat gatctgaagt tagcctagga tagcggtctt tcaaggttaa
     9901 gcaagtcata atagctaaag ctgcattaga cacttggtgg tgtcctggca tagcgatgtg
     9961 aatatctgaa ataactccta aaggactagt aaaacgatag ccagatgcgt tctcagccat
    10021 ttgaaactgc tcttgccatt cccagattga agcaccaacc tgctcagctt ttgttaaaaa
    10081 aacctctcga gctgacgggt tttcaacggc aaaaacaagg gtctcgcccc cttctaaaac
    10141 accagctttt tgggcggcaa tatcagcgta agtctctccc aaaatagctt ggtgatctaa
    10201 accaattgag ggacatacca caaccattgc ttgaaaaaca ttggtcgaat cataaagacc
    10261 gccaaggcct gcttcaataa tagcaatatc tacaggatgc atgtctccaa aatagaggaa
    10321 catgatcaaa gtaatcactt caaattcagt tacctctcca aaatcagtct cttgaactag
    10381 acgctctgtc aaaggacgaa tacgattagc agcaataacc aaatcttttt ctgaaatcat
    10441 tcgaccattt atgctaatac gttctttgaa atccataata taaggagagg taaaggtccc
    10501 cacttcataa ccagctgtcg taaaaatgtg ttgtaaatga ttgaccgtgg aaccttttcc
    10561 gttagttcca acgatatgca ctcccttgac atttttttgg ggatttccta actgtccaag
    10621 aacccaaagc atacgtttta agcctggctt gatgccgaaa actaagtgat catgaatcca
    10681 ttctagagtc tcttcataag tcatttcaca cctcacatct atctttagta taccatatat
    10741 aaaaaaaagt gacagcagat aacctagagt agtacccttt ttttattatc ctcaccaaac
    10801 actcaaataa gttctaaagt acactacact tttatcttca ttgctttttt tacgtgataa
    10861 gaataccagc cccacatagt ctttgctacc tcctaaagtg ttatctgtga caataatttt
    10921 tttactcctt aatacacgtt cttccaaaaa acaagaaaaa aggctgactt caagccttct
    10981 tttctctaaa aaatgttaca atagaggtac ttatatcaaa aaaaggagca agtaatgaac
    11041 aatagacata aacggcgcca aaaaagaaaa gccttcactc tcattaacct tatactatta
    11101 tttgcctgca ttcttggttt gaccctcatt ttctatttat accaagggtc taacaaattc
    11161 ggcttgtcag atgtctctca atttgtacct aaaataagtc tttttcatca caaaaagacg
    11221 gcaaaaaaag aaacgacaaa acttaagaag actcatttcg attcctcaaa atctcaaaaa
    11281 aaggcccatt ctaaattaac ttggaccaag caagaaacac ctgtcaagat ccccatttta
    11341 atgtatcatg ctattcatgt aatgtcccct gaagagacgg ccaatgctaa tttgattgtc
    11401 aacccagatc tctttgatca acaacttcaa aaaatgaaag acgagggcta ctattttttg
    11461 agtcctgaag aggtctaccg cgctctttca aataatgaat tgcctgctaa aaaagttgta
    11521 tggctaacat ttgatgacag tatgattgat ttctacaacg ttgcttaccc aatcttaaaa
    11581 aaatatgatg ctaaagccac caataatgtt attacaggac taaccgaaat gggaagcgct
    11641 gccaatctga ctcttaagca gatgaaagaa atgaaacaat tcggtatgtc tttccaagat
    11701 catacagtga atcatcctga tctagagcag gctagtcctg atgttcaaac aactgaaatg
    11761 aaagattcaa aggactatct tgataagcaa ttaaaccaaa acactattgc cattgcttat
    11821 ccatctggta gatataatga cactacctta caaattgcag ctagacttaa ctataaatta
    11881 ggtgttacca ctaacgaagg aatagctagc gcggctaatg ggcttctttc tcttaacagg
    11941 attcgtatcc tcccaaacat gagtccggaa aatcttttac aaacaatgga accataacat
    12001 caattttttt atcattccaa atcatactca ctgtaaacta aaaagacagt tatgaaaaaa
    12061 ttctttcata actgtctttt tttgattgtt ttaaaattat tttattctta tctttaacgt
    12121 cttttttcaa gaaaatctcg ccaccacagc tgtaagtcag aggctttttt aagagattgg
    12181 ctagctttaa gaggaatacg cttaggttcc tttaaatacc tcactgactg atctgttatt
    12241 cttggagacg tcaaataagc aaccacttca cctttagtaa tcggtgctag taaagaagtc
    12301 tctgatatca taaattgttt aggtttggga acttgttcac cctgttgcaa aatgatggtt
    12361 acatcttcgg caacagatac cggtagttct tttacttggc catcttgaat agggagcgac
    12421 cgctccgaga caagacttcc ttttttaaga atcttacgac gacaataagt tctagccaca
    12481 tagtccaaca aacgatttgc ttgctcaaac cgcgtgtttt ggtttttatc agatccatcg
    12541 gcatttagaa taacggtaat cactcgcata ccattttcaa tagaagttac cacaagacaa
    12601 tgacctgcca agtctgttgt cccagtcttt aaaccatcca ctcctgctct acccttagcc
    12661 atacctggta agagttgatt aaaactttta atagaattgc cagcaaaatt aacttctgtt
    12721 tgtttggtga tctctaaaat ctcaggaaaa tcttctaaca aatgcatcgt cacaactgca
    12781 acatcttgag cactcaaggt attttcttct tccaacgcag aaccaggata acgattatcc
    12841 tttaacacct catttggcag acctgaggcg ttgaggattt taccagaagt aatcccccaa
    12901 tgagatagct gttctctcat ttgatttaca aaatgaagct ctgaacccgc cactttttcg
    12961 gctaacgcaa tagcagcgct atttgagctt gctaccaaag tagctgtcaa caggtcttga
    13021 actgaataag tttttttgtc aaaaggtaca ttacttaaag acctatcttt tgttaactca
    13081 aaggcataat ctgagagatt aacttgatct gataattgca gctggcctga tttaacctta
    13141 tctaaaacca gataagccgt caccaatttg gtcaaagagg caatagcatc tggcgtctta
    13201 gcgtctttct catacagaac tctcccagag tctgcctcta cgacaatcgc atgttctgct
    13261 cccacttgaa aatcagctgc gagaactgtt ttaccaaacc ctgaacaagt taataccata
    13321 aaaaaaagga taacccacct ttttatcatt atatctttca tagttactat tataacaaaa
    13381 attctcagaa atttggaagg aagtatagtg gcacacacct ttagtagctt aaacaagctc
    13441 ataaagcaag taccctgtga caataacaca tcctgttata tgggcgatat aataagcttc
    13501 gttatatcaa ttagttgttt ggcaggaatt ttggtataaa atggaatcta atcctgaact
    13561 tattacattt tgggttattt cttcagatac tgttttatct aatgcattaa accagtgact
    13621 atatgtattc aaagttgtag acttatcagc atgtcccata cactttgcaa cgtacagaat
    13681 gtcttttttc aaaacattaa taagatagga agcatgacta tgtcgcaaga cttttcccgt
    13741 tatcactggt actcctactt cttgagcttt gtgcttaata actcgacaaa tcgttgattt
    13801 acaaaatggt tcaccaaacc gtgaaataat gaaatctgtg tcagtattga ctacttgatt
    13861 tcttctccaa ttttccaata ctactattgt cacatcatct aactcaataa gacgttctcc
    13921 tgctggcgtt tttgtttgat ctttacgata ccaatttcca ttttcatcct tttccaaggt
    13981 tgtatgaact ttcaaaaatt tttgttcgaa atcgatatct tcccatcata atgataaacc
    14041 ttcacttaca cgaacacctg tcatataata tagccaaata gttgtaaatc tttgtagctc
    14101 ttcataatct tgcaaatcaa acgattttat aaaattctga aactcaatat aagtccaaaa
    14161 tggagtatcg ggatgtttcc ctttaggatt atccaaagcc ttacacggca cttttgaaat
    14221 atagtctaac ctttcagcat accccatgca agctttgaat cgcgaccata aattctttgc
    14281 ataattttca a
//
LOCUS       DAUCWO010000043        14283 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUCWO010000043 DAUCWO010000000
VERSION     DAUCWO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.043"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            92..1288
                     /locus_tag="VGY20_001225"
     CDS             92..1288
                     /locus_tag="VGY20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ4274076.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALAGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            1551..2102
                     /locus_tag="VGY20_001226"
     CDS             1551..2102
                     /locus_tag="VGY20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ4274077.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKTGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWLNKKHSDVR"
     gene            2169..2540
                     /locus_tag="VGY20_001227"
     CDS             2169..2540
                     /locus_tag="VGY20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ4274078.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            2578..3924
                     /gene="glnA"
                     /locus_tag="VGY20_001228"
     CDS             2578..3924
                     /gene="glnA"
                     /locus_tag="VGY20_001228"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ4274079.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(4152..5834)
                     /locus_tag="VGY20_001229"
     CDS             complement(4152..5834)
                     /locus_tag="VGY20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ4274080.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDILALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(5836..6066)
                     /locus_tag="VGY20_001230"
     CDS             complement(5836..6066)
                     /locus_tag="VGY20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ4274081.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            6350..7048
                     /gene="tsaB"
                     /locus_tag="VGY20_001231"
     CDS             6350..7048
                     /gene="tsaB"
                     /locus_tag="VGY20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ4274082.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHASLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            7020..7475
                     /gene="rimI"
                     /locus_tag="VGY20_001232"
     CDS             7020..7475
                     /gene="rimI"
                     /locus_tag="VGY20_001232"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ4274083.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKLIGKRPDYYRNPIETALLMKREGKHDR"
     gene            7465..8493
                     /gene="tsaD"
                     /locus_tag="VGY20_001233"
     CDS             7465..8493
                     /gene="tsaD"
                     /locus_tag="VGY20_001233"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ4274084.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(8664..8933)
                     /gene="rpsN"
                     /locus_tag="VGY20_001234"
     CDS             complement(8664..8933)
                     /gene="rpsN"
                     /locus_tag="VGY20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ4274085.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(9168..9911)
                     /locus_tag="VGY20_001235"
     CDS             complement(9168..9911)
                     /locus_tag="VGY20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4274086.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSCIPIEIISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSC"
     gene            10151..10930
                     /gene="udp"
                     /locus_tag="VGY20_001236"
     CDS             10151..10930
                     /gene="udp"
                     /locus_tag="VGY20_001236"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ4274087.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            10951..12153
                     /locus_tag="VGY20_001237"
     CDS             10951..12153
                     /locus_tag="VGY20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ4274088.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            12183..12854
                     /gene="deoC"
                     /locus_tag="VGY20_001238"
     CDS             12183..12854
                     /gene="deoC"
                     /locus_tag="VGY20_001238"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ4274089.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KPDSY"
     gene            complement(12949..14118)
                     /locus_tag="VGY20_001239"
     CDS             complement(12949..14118)
                     /locus_tag="VGY20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4274090.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYSYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDVLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSADQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVTDFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
BASE COUNT         4351 a         2418 c         2981 g         4533 t
ORIGIN      
        1 aaatgattat tttattattt catattttat ctttatgcta ggaattgtgg tataatggag
       61 gagtataaaa aatattaagg agtctattca catggctaaa ttgactgtta aagacgttga
      121 tttgaaaggt aaaaaagtcc tcgttcgtgt tgactttaat gtgcctttga aagacggcgt
      181 tatcactaac gacaaccgta tcactgcggc tcttccaaca atcaagtata tcatcgaaca
      241 aggtggtcgt gctatcctct tctctcacct tggacgtgtt aaagaagaag ctgacaaaga
      301 aggaaaatca cttgcaccgg tagctgctga tttagctgct aaacttggtc aagatgttgt
      361 attcccaggt gttactcgtg gttcaaaatt agaagaagca atcaatgctt tggaagatgg
      421 acaagttctt ttggttgaaa acactcgttt tgaagatgtt gacggtaaga aagaatctaa
      481 gaatgacgaa gaacttggta aatactgggc ttcacttgga gatggaatct tcgttaacga
      541 tgcatttggt acagcacacc gtgctcacgc atcaaacgta ggtatttcag caaacgttga
      601 aaaagctgta gctggtttcc ttcttgaaaa cgaaattgct tacatccaag aagcagttga
      661 aactccagaa cgtccattcg tagctattct tggtggctca aaagtttctg ataagattgg
      721 tgttatcgaa aatcttcttg aaaaagctga taaagttctt atcggtggtg gtatgactta
      781 cacattctac aaagctcaag gtatcgaaat cggtaactca cttgtagaag aagacaaatt
      841 ggatgttgct aaagacctcc ttgaaaaatc aaatggtaaa ttgatcttgc cagttgactc
      901 gaaagaagca aacgcatttg ctggttacac tgaagttcgc gacactgaag gtgaagcagt
      961 ttcagaaggc ttccttggtc ttgacatcgg tcctaaatca atcgctaaat ttgatgaagc
     1021 acttgctggt gctaaaacag ttgtttggaa tggtccaatg ggtgtctttg aaaaccctga
     1081 cttccaagct ggtacaatcg gtgtcatgga cgctatcgtt aaacaaccag gagttaaatc
     1141 aatcatcggt ggtggtgatt cagcagctgc tgctatcaac cttggccgtg ctgacaaatt
     1201 ctcatggatc agtaccggcg gaggcgctag catggaactt ctcgagggta aagtattacc
     1261 aggtttggca gcattgactg aaaaataata gtcattataa tatatagtaa agggcatgct
     1321 gatgcatgtc tttttttagt tgatcatgta gagttattga actttcccta acttcactta
     1381 tcatttacta gtttttagat atagtggtat ttggctgaca cttcaatttt caaaagttta
     1441 tgacgatttc atatcaattg gtttataaaa gatatcctaa tcataaataa aacgtttacc
     1501 aaaagggttt tatggattga tatgttaaaa tagatagtga agggataggt atgaaaacgt
     1561 taagaaaatt attatctaat tacaagtttg atattaaaaa gtttaaattg ggaatgagaa
     1621 cattgaaaac aggcttatcg gtctttttag ttttattggt ttttcattta tttggttgga
     1681 aagggctcca aattggtgct ctaactgctg tatttagttt aagagaagac tttgataaaa
     1741 gcgtgcactt tggcttttct agaattattg gtaatagtat aggtggccta ctctccttag
     1801 tattttttgc attcaatgag atatttcatc aagctttttg ggttacttta ttaattgttc
     1861 ctatttgtac catgttatgt atcatggtta atgttgcatg taataataaa acaggtatta
     1921 ttggggcagt ggctgctctt ttgattatta cactgtctat tccaacagga caaacgttta
     1981 tttatgtgac ttcgcgggta tttgaaacat tttgtggcgt ctttgtagct attttagtta
     2041 atactgatgt tgaacttatt aaaaataaat ggcttaacaa aaaacacagt gatgttagat
     2101 aatataacat atatacttga caggttattt ttttcagcct ataataagga agatgaaagg
     2161 agtttgtcat gaaagaaaaa gaacttagac gctcaatggc tgtttttcct attggaacgg
     2221 tgatgacatt aacagatctc tctgcgagac aaattcgcta ttatgaagat caaggtttaa
     2281 ttaagcctga acgaacgcaa gggaatcggc gtatgttttc cttaaatgac atggaccgac
     2341 ttttggaaat taaagatttt ttgtctgagg gattaaatat tgcagctatt aaacgcgaat
     2401 atgtcgagcg tcaaggtaag ttaatgcaaa aacaaaaagc tctaacagat gcagatgtca
     2461 gacggatctt acatgatgaa atgctgacac aaagcggttt ttctactccc tcacagcata
     2521 ttggtaattt tcgtatttaa gatatgaatt gtataaaatt taaaggagac ctatttaatg
     2581 gcaatcacag tagctgacat tcgtcatgaa gtcaaagaaa aaaatgtaac gtttcttcgc
     2641 ttgatgttca ctgatatcat gggcgttatg aaaaatgtgg agattcctgc aacgaaagaa
     2701 cagttagaca aagtattgtc taataaggtt atgtttgatg gttcatctat cgaaggtttt
     2761 gtacggatca atgagtcaga tatgtacctt taccccgatt tagacacttg gattgttttt
     2821 ccctggggag atgaaaatgg agcagttgca ggtttaattt gtgatattta tacagcagaa
     2881 ggaaagcctt ttgcaggaga tcctagagga aatttaaaaa gagccctgaa acacatgaac
     2941 gagatcggct acaaatcatt taatcttgga ccagaaccag aatttttcct ttttaagatg
     3001 gatgataaag gtaatccgac acttgaagtt aacgataatg gtggttattt tgatttagcg
     3061 ccaattgact tagcagacaa cacgcgccgt gaaattgtga atattttaac gaagatgggt
     3121 tttgaagtgg aagctagtca tcatgaagtg gctgttggtc aacatgagat tgattttaaa
     3181 tatgcagatg ttttgaaagc ttgtgataat attcaaattt ttaagctagt tgtaaaaacg
     3241 attgcccgtg aacatggact ttatgctact ttcatggcta aaccaaaatt tggaatagct
     3301 ggatcaggga tgcactgtaa catgtctttg tttgataacc aaggtaataa tgctttttat
     3361 gatgaagctg ataagcgagg aatgcagtta tcagaagatg cttattattt cttgggagga
     3421 ctaatgaagc atgcttataa ctacactgct atcactaacc ctacagtgaa ttcttataaa
     3481 cgattagttc caggttatga ggcacctgtt tatgtcgctt gggctggaag taatcgttca
     3541 ccgcttatcc gtgttccagc atcacgtggt atgggaacgc gtttggagtt acgttcggtt
     3601 gatccgacag ctaatcctta tttagccttg gctgttctct tggaagctgg attagatggt
     3661 atcattaaca aaattgaagc tccagaaccc gttgaagcta acatttatac catgacaatg
     3721 gaagaacgaa atgaagcagg cattattgat ttgccatcaa cgcttcataa tgccttaaaa
     3781 gctcttcaaa aagatgatgt ggtacaaaag gcactaggtt accatatcta cactaatttc
     3841 ttagaagcaa aacgaattga atggtcttcc tatgcaactt ttgtttctca atgggaaatt
     3901 gaccattata ttcataacta ttagacttat caggacctgc tcaagttgag tgggtctttt
     3961 ggatgagcta ttgtatttga gctagcaagc tattattagc tgatcggaca ccaattgatt
     4021 ttccattata gaaaactaaa aaccagtcca tgttctatga ggactggttt ttagtttgat
     4081 aattatctga actagaactg atatattaaa gatatagacg gatagtatta agaaatgagt
     4141 cttatttgat tttagtgctt atcaggtgtt aagaccatcg gaattataat tggttcacgc
     4201 tcagtctttt catacaagaa tggacgaaga gcattgacga tagcaccgtt aactgattgg
     4261 atgctagcat ccttattttt taaagcaata cggatagcgt taaagagcac acgttggctt
     4321 tcgcggatca agtcacctga ctcacgcata taaatgaaac cacggctcag aatatctggt
     4381 ccagctaaaa tcatttgtgt gttaaaatca acagttgcaa cagctaagac aacaccatct
     4441 tctgacaaat cacggcggtc ccgtaaaacg gctgctccaa tatccccgat gccattacca
     4501 tccacgtaga tgtcttgggc attaaagtga ccagcaatac gcgcactgtc actggttaaa
     4561 gctagaatgt ctccattttc cataataaag atattttctt ttggaatacc aatatccatc
     4621 gccagtcctg catgaacttt ttgcatgcga tactcaccat gtactggcat gaaatatttt
     4681 ggtttaatca aactaagcat gagcttttgt tcttgctgtc ctccatgacc agaagtatga
     4741 atattattga cttttccatg aataacatca acaccagctt cttggatggt attgattaat
     4801 ttattgacac ttgtggtatt accagggatt ggacttgatg agaaaatcac agtatcacca
     4861 ggctgtaaag ttacttgacg atgagtgcca ttagcgatac gagcaagtgc tgccatagac
     4921 tcaccttgac tacctgtaca cataattaaa acttcactag catgaagatt tttaagttcg
     4981 cttggctcaa tgaaggtacc ttttggaact ttaatatagc caagttcaat accgttaaca
     5041 attgcctttt ccatagaacg accaaaaacg gcaattttac gtcccgtttt aacagcagct
     5101 tctgcagcct gttgcaaacg gtagatgttt gaagcgaagg aagcaaaaat aatacgccca
     5161 tgaatacctt cgataatttt taaaatggat tggccaacaa ctttttcaga gttagtaaag
     5221 gttgggattt cagcattagt tgagtctgat aataagcaaa ggacaccttc ttcaccaagc
     5281 gctgccatac gttgtaaatc tgcaggatct cctactggcg taaagtcaaa tttaaaatca
     5341 ccagtacaga taatcttacc ttgaggggtg tggataacaa taccgaccgg ttctggaatg
     5401 gaatgagtag tcttgaagaa agtgacgctc atgtttttaa aagttaactc ggtgttgtga
     5461 ttgatttcgt aaacagttgc ttcacgccaa agaccatgtt cttccaattt accacggata
     5521 agagcaagtg ctaaaggtcc tgcgtagata gggatattag cctgtttgag aagaaatgga
     5581 ataccaccaa tgtggtcttc gtgaccatgt gtaataacaa gagctttgac ccggtctaag
     5641 ttatcgacaa tgtaagaata gtctggaata acgtaatcaa ttcccaaaag atcatcttct
     5701 gggaatttga taccggcatc aacgataatg atctcgtctt gatactcaat accataagta
     5761 ttcttcccaa tctctcccag accaccaatg gcaaacaccc caacttcatt aggttttaaa
     5821 ctgatatttg tcatcttaga actccgttaa ttcaaaaaca ccggtctctt tttcgtaatc
     5881 gaggtgtttg tcagaaagta gctcgataaa ttccacgtta taataggtgt tatcttcaac
     5941 gagacgacga gccttgatac ggccatcgag ttcatcagta gcatcaatat taagatagag
     6001 tgctttagta ctttcacgac gtgggctttg atcctttgtt tcttgataga aaactttata
     6061 aatcatttgt tttttattcc tttcgttatg aatcaattat ccaaacaata ataaataggt
     6121 caaaaggtag gtgttccatt ttgacggttt tatatgttgt cattattgtg tagtagatta
     6181 attcctgatt ctgtgcaatt attataatta taccataaac ttagctttta gtaaaaaatt
     6241 taggtacatt tatggtatag tctaaatcaa attaagagtc agcctttttg tttatgattc
     6301 ttaatttgtg ttataattga tactagaact tgcagtagaa aggcataaaa tgaagacact
     6361 tgcatttgat acctcaaata aaaccttgtc ccttgctata cttgatgatg agacacttct
     6421 agcagatatg acccttaaca ttcagaaaaa tcatagcgtt agccttatgc ctgctattga
     6481 ttttttgatg acttgtactg atcttaaacc tcaagattta gaaagaatag tggttgcaaa
     6541 aggccctgga tcttacacag gtttacgagt ggcagttgct actgcaaaaa cgttagcgta
     6601 cagtttaaat attgcattgg tcgggatttc gagtctatat gctttggctg cgtctacttg
     6661 taaacagtat ccaaatactt tggtggtgcc attgattgat gctagaaggc aaaatgcgta
     6721 tgtaggttat tatcggcaag gaaaatcagt gatgccacaa gcccatgctt cactagaagt
     6781 tattatagaa caattagtag aagaaggaca gctgattttt gttggggaga ctgctccttt
     6841 tgctgagcaa attcaaaaga aactacctca ggcgatacta cttccaaccc ttccttctgc
     6901 ttacgaatgt ggtcttttgg ggcaaagttt ggcaccagaa aatgtagacg cctttgtccc
     6961 tcaatatctc aagagagtgg aagctgaaga aaactggctc aaagataatg agataaaaga
     7021 tgatagtcac tacgttaagc gaatctaata tgaaaacagt tgaagaacaa gcaaaaaata
     7081 tttatcagct tttggaaatg gtttatggca cgtctccttg gactttagaa caagtattaa
     7141 tcgatatacg gcgggatcaa acagattatt tcctactata tgatcatgat aaattgctag
     7201 gttttttagc tattcaagat ttagctggtg aagttgaaat gactcaaatt gctatactac
     7261 ctagtcatca aggacttggg ctagcgtctc aattgatgac gcatttagat agcattgaaa
     7321 gtgacatctt tttagaggtt agagaatcta atcatagagc acagggttta tatcagaagt
     7381 ttggttttaa acttattggc aaaagaccag actactatag aaatcctata gaaacagctt
     7441 tattgatgaa acgggaagga aaacatgaca gataggtata ttttagcagt tgagagttct
     7501 tgtgatgaga ccagtgtagc gattttaaaa aacgaaagta ccctcttaag taatgtcatt
     7561 gcaagtcagg ttgagagcca caaacgcttt ggtggtgtgg tgccagaagt agctagtaga
     7621 caccatgtcg aagtgattac gacttgcttt gaggatgctt tgcaagaagc tggcatcagt
     7681 gcttcagatt tatcagcagt tgcggtaact tatggccctg gacttgttgg agccttgtta
     7741 gttggattag cagctgcgaa ggcttttgct tgggcaaatc atttaccttt gattccggtt
     7801 aatcatatgg caggccactt aatggcagcg cgtgaacaaa agcctttagt ttatccttta
     7861 atagcactat tggtttcagg aggacacaca gaattggttt atgtcccaga gccaggggat
     7921 tatcatatta ttggcgagac gcgtgacgac gctgttggtg aggcttatga taaagttggg
     7981 cgtgtgatgg gcttaaccta tccggcagga cgtgaaattg accagttggc tcataaaggt
     8041 caagatactt atcatttccc tcgtgctatg ataacagaag accacttaga attttccttt
     8101 tcaggactca agtccgcttt tatcaatctt catcataatg ctaagcaaaa aggcgatgaa
     8161 ctgattctag aagatttgtg tgcatctttt caagcagctg ttctagatat tttattggcc
     8221 aaaacaaaaa aagctctgag cagataccct gcaaagatgc ttgtagtggc tggtggtgta
     8281 gcggcaaatc aaggattacg agatagatta gcacaagaaa ttacacatat agaagttgtg
     8341 attcctaaac taagactctg tggagataac gcgggtatga ttgctttagc agcagctatt
     8401 gagtatgaca aacagcattt tgctaatatg agcctaaacg cgaagcctag tttagctttt
     8461 gaccagtttc cagacagttt tgtaataaac tagtcacact tgctagttag taagatttat
     8521 aatggataag aatctccctt tgaaaaaaca acccggtatc ttggcatacc gggttgtcat
     8581 gatgataagt aattggcttc tatggttact aataattcag ataattacct gcaaaataat
     8641 tgtctgccta aggtgaagta agcttaccag ctagctttag tgacgccagg aagctgtcct
     8701 ttgtgagcta agtcacggaa attgatacgg ctaacaccaa acttacgcat gtaagcatga
     8761 ggacggccgt caattttgtc gcggttcttc aagcggtttg gatttgaatc acgaggcaat
     8821 ttgcgcagtg attcataatc gcccttcgct tttaagtcac gacgaaggtc agcgtactgt
     8881 tcaatcagtt ggagctgctt ttggtattta gcaatttttg acttttttgc cattgctttc
     8941 tccttcttaa tatttaataa cctcagagtc gttatcagcg ctctgaatcc acttaactgg
     9001 tatttaactg gttaatcgga tatataatca ttatacaata aataaaagca aaagcaacta
     9061 aaaaaaatta actagttatt tttttagcca taatatcaaa aaactccaca tctaagtgtg
     9121 ggagtcaggt attaataact gtttaaaggt gtttaatgag tgtcgcttta gcaactttga
     9181 gattgaactt ttaagtcaaa taaatcagca ggaacgtagt gtttggttga gaccaagatg
     9241 tctttaccat tgtagagagt ttcttggatt aaaataaaag cactattttc tgatagagta
     9301 tacttagttg ctgtgtaatt gccagtcttg gtatagccga tttggttgca ggaatgagaa
     9361 gctttaccag attcatagat tttctcttct aagaaagtaa gaaggggctc ttcttgattg
     9421 agatttatag catatttaga aataatctca atagggatac aagataaact ataagcaata
     9481 gctttatttt ggctatgata ccagcgatca acaataatca caacaggagt ttcttgtttt
     9541 agggatgctg taatggcaac agtgggcact tcaatccgat attccaactc aacctttgtg
     9601 atgtcagatg ataggcttgc atatatgggg tgttgaagat attcgaaacc ttggtgatat
     9661 ttagtttcag gagtcttgag tataaaattt ccctttcctc tgatattttt tatgagatga
     9721 tcctcttgga ggagggctag ggatttacgt agtgtcatcc gactgacatt gagctgtcta
     9781 gcaagttcag gttctgatgg cagttgcatg ccaggactat atgtaccatc ttgaataagt
     9841 tgaaaaatag tattatatac ttgcacatgt ttaagttttt tgagtttttt ggttaagtcg
     9901 ttagtagaca tatcacttac ctcattaata atctttagtg gagtttaact tgtctaaaaa
     9961 ctattttagc actaacagta ttccttgatt agaattgtac ccatctatag tgttagtatc
    10021 tcacactcag gtactttttg tcaaaaaaga gatgaaaaac accttaaaaa agcgtttaca
    10081 aaatagagtt tgtgtgatac tatacaagtg tatcaaaaat tagacaagta aaatacagga
    10141 ggtttttaac atgcaaaatt attcaggtga agtcggattg caatatcacc tacagattcg
    10201 tccaggtgat gtaggtcgtt atgttatcat gccaggtgat ccaaaacgtt gtgcaaaaat
    10261 tgcagaacat tttgataatg cagttcttgt agcagacagc cgtgaatatg ttacgtatac
    10321 aggtacatta aacggtgaaa aagttagtgt tacttcaact ggtattggtg gtccatcagc
    10381 gtctattgcg atggaagaat tgaaactttg tggcgctgat acctttatcc gtgttggaac
    10441 atgtggtggt attgaccttg atgttaaagg cggagacatt gttattgcaa ctggagctat
    10501 ccgtatggaa ggtaccagta aagaatatgc tccgattgaa tttcctgctg tagctgactt
    10561 ggaagtgaca aatgctctag ttaatgcagc taaaaagctt ggttatacaa gtcacgcagg
    10621 agtagttcaa tgtaaagatg cattttacgg gcaacatgag ccagaacgta tgccagttag
    10681 ttatgaatta ttaaataaat gggaagcatg gaaacgtctg ggaacaaaag cgtctgaaat
    10741 ggaatctgca gctctttttg tagcagcaag tcatctagga gttcgttgtg gatcagattt
    10801 ccttgttgtt ggtaatcaag aacgcaacgc tttgggtatg gataacccaa tggctcatga
    10861 tacagaagca gctattcaag ttgctgttga agcactccgc acacttattg aaaatgataa
    10921 atcacaataa gcgaggtttt ggaagtcatc atgcaattta tttatagtat tattggtatt
    10981 ttattggtat taggaattgt gtatgcaatt tctttcaatc gtaagagtgt ttctctaagt
    11041 ttaattggaa aagctcttat cgttcaattc attattgcgc taatcttagt acgtatccca
    11101 ctaggccaac aaattgttag tgttgtttca actggagtta ctagcgtaat caactgtggt
    11161 caagctggtt taaattttgt gtttgggtca ttagcagata gtggcgcaaa aactggtttt
    11221 attttcgcta ttcaaacgct tggtaatatt gttttcttat ctgccctagt tagtctactt
    11281 tattatgtag gaatccttgg atttgtagta aaatggatag gtaagggcgt tggtaaaatt
    11341 atgaaatcct cagaggttga gagttttgtt gccgtagcta atatgtttct tggtcaaaca
    11401 gacagtccaa ttttggttag caaataccta ggtcgtatga ctgatagtga gataatggtt
    11461 gtgttggtat caggtatggg aagtatgtca gtttctattc ttggtggcta tattgcatta
    11521 ggcattccaa tggaatatct cttgattgct tcaacaatgg ttcctattgg cagtattctc
    11581 attgctaaaa tcttattgcc tcaaacagaa cctgttcaaa aaattgatga cattaagatg
    11641 gataataaag gtaataacgc caatgtgatt gatgcaatcg ctgagggtgc aagcacaggt
    11701 gcacaaatgg ctttctcaat tggtgctagt ttgattgcct ttgttggttt agtttctttg
    11761 attaatatga tgttaagtgg attgggaatt cgcttagaac aaatcttctc atatgttttt
    11821 gctccatttg gttttcttat gggatttgac cacaaaaaca ttcttctaga aggaaacctt
    11881 cttggaagta agttgatttt aaatgagttt gtttcgttcc aacaattggg tcacctaatc
    11941 aaatctttag attatcgtac agcattggta gcaactattt cactctgtgg ttttgctaat
    12001 ttatcaagtt taggtatttg tgtttcaggt attgctgttc tttgcccgga gaaacgtggc
    12061 accctagctc gacttgtttt ccgtgcaatg attggcggta ttgctgtaag tatgcttagc
    12121 gcctttatcg tcggtattgt aactctattc taaacgttga caaaagaaag aaggatagta
    12181 acgtggaagt aaaagatatt ttaaaaacgg tagaccatac tttgctagca acaacagcaa
    12241 cgtggccaga aatccaaaca attttagatg atgccatggc ttatgaaaca gcttcagcat
    12301 gtattccagc ctcttacgtc aaaaaagcag cagaatacgt ttcaggtaaa ttagctattt
    12361 gtactgttat tgggttccca aatggctata gtacaactgc agcgaaggtt tttgaatgtc
    12421 aagatgctat taaaaatggt gctgatgaaa ttgacatggt cattaatttg acagacgtta
    12481 aaaatgggga ttttgatact gttgaagaag aaattcgtca aatcaaagcc gcatgtcaag
    12541 accatatctt aaaagttatc gttgagacat gtcaattaac taaagaagaa cttatcgaac
    12601 tttgtggagt tgtcacacgt tcaggtgcag actttattaa aacctctact ggtttttcga
    12661 cagcaggtgc tacatttgaa gatgttgaag tgatggcaaa atatgtcggc gaaggtgtta
    12721 aaattaaggc agcaggtgga atctcatcat tggaagatgc taaaacattt attgctttag
    12781 gagcttcacg cttgggtact agccgtatca tcaagattgt taagaacgaa gctacaaaac
    12841 ccgatagcta ttaacttaat cataccgatt tagcttgaga ctaaaacaaa aaactcatgt
    12901 aataggagtt aaaaagccta ctatcttagt gataataggc ttttttagct atgaagactt
    12961 ccctgcgatc catcctgaac aaagagctga ggtaatatta aaccctccgg tgtgagcatt
    13021 aatatctaag acctctccag cgaaatacag gccaggaact ttcttgcttt ccaaggtttt
    13081 agggttgatt tcttttaaat ctacgcctcc tttggtcaca aacgatttag ccaaagacat
    13141 ttttcctgta atgggtatct ggaggtgttt gagtttatca agcaattcct tttcttgttt
    13201 tggagagagt tgtttgacct tttcggggta atcttctgat aaaaaatcag ttactcgttc
    13261 aggcagtagg cctttaagag catttttgat gtttttatca cgttgatcac tcagataagc
    13321 ggttaagtca tctgttgata agtgtggcaa aaagtccagc tctgcaattt ctccaccttt
    13381 cacaaaagag gacagacgaa gagctgcagg tcctgaaagg ccaaagtggg tgaagagtaa
    13441 gtcgtgtgtg atgacgtgtt tgtcataact aagagtaacg tcgtctaggg aaataccttg
    13501 caagactttg tgaggaaaat cagttaacag agggctctca gcagcttcta aatcagtcac
    13561 ggtcagttta aaatgccgtg caatatcatg tccaaaacct gttgaacccg ttgaagggta
    13621 ggacttgcct cctgtggtca caatcaattt ttggcaggtg aaagtctggt ctgcagattt
    13681 gaggtagaag aggtcatctt gttttttaac ggaaaccact tcagtgctgg ttagcacctg
    13741 acccccaaga gccttgattt ttttctccag agcatcaatg attgtccttg atttatctgt
    13801 tgtggggaac atccgtccat ggtcttcttc tttgagtttg acgccgtttt cctcgaaaaa
    13861 ggcaatgatg tcatggttgt caaactggga aaaaacacta tataaaaagc ggccatttcc
    13921 aggaatgcca gccataagga catcaaggtt accactatta gtgacattac agcgtccacc
    13981 accggttccc gctaattttt tacctaagcg gcgatttttt tcaattaaga gagttttata
    14041 gctatagtag ctgctggaga tggcggccat cataccagca gggccacctc caataataat
    14101 cgtatcgtat tgagtcatag ccttatttta gcataaaagc gggggattta atagtcaggt
    14161 aaatgaacta acatggcata ccgtttttta cgcatttaaa caatgcctcc atgacatcaa
    14221 aaagctcgaa aaatagtcca gatgattaaa attatctttg tatgaaaacg gttgcttttt
    14281 tat
//
LOCUS       DAUCWO010000044        13507 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUCWO010000044 DAUCWO010000000
VERSION     DAUCWO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.044"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..858
                     /locus_tag="VGY20_001240"
     CDS             <1..858
                     /locus_tag="VGY20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ4274091.1"
                     /translation="TDDLKPFQVKDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKES
                     NSLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKK
                     LVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANH
                     TWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQML
                     WTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYLKAEGYECV
                     TVSELYAHQ"
     gene            complement(968..2053)
                     /locus_tag="VGY20_001241"
     CDS             complement(968..2053)
                     /locus_tag="VGY20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4274092.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVNQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDINNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2151..2777
                     /gene="udk"
                     /locus_tag="VGY20_001242"
     CDS             2151..2777
                     /gene="udk"
                     /locus_tag="VGY20_001242"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ4274093.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2857..3120
                     /locus_tag="VGY20_001243"
     CDS             2857..3120
                     /locus_tag="VGY20_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ4274094.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3173..3982)
                     /locus_tag="VGY20_001244"
     CDS             complement(3173..3982)
                     /locus_tag="VGY20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ4274095.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4127..4624
                     /locus_tag="VGY20_001245"
     CDS             4127..4624
                     /locus_tag="VGY20_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ4274096.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4624..6294
                     /gene="dnaX"
                     /locus_tag="VGY20_001246"
     CDS             4624..6294
                     /gene="dnaX"
                     /locus_tag="VGY20_001246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ4274097.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            6389..6592
                     /locus_tag="VGY20_001247"
     CDS             6389..6592
                     /locus_tag="VGY20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ4274098.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
     gene            complement(6567..7508)
                     /gene="birA"
                     /locus_tag="VGY20_001248"
     CDS             complement(6567..7508)
                     /gene="birA"
                     /locus_tag="VGY20_001248"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEQ4274099.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGYLIVQFENGDLQTLRSGEISLSSWEN"
     gene            7712..10090
                     /locus_tag="VGY20_001249"
     CDS             7712..10090
                     /locus_tag="VGY20_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ4274100.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSCKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQRAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSRLVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KDWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGLGFTPIKDISPVLQFKKLKQLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHTYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            10627..11823
                     /locus_tag="VGY20_001250"
     CDS             10627..11823
                     /locus_tag="VGY20_001250"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ4274101.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            11997..13256
                     /locus_tag="VGY20_001251"
     CDS             11997..13256
                     /locus_tag="VGY20_001251"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ4274102.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
BASE COUNT         4311 a         2328 c         2808 g         4060 t
ORIGIN      
        1 acagacgact tgaaaccgtt tcaggtaaag gatctagtat caggacattt agaaagaata
       61 caagaagaag ttgaaaaaaa atatccaaat gctggtttta atagcgataa gtacaatggc
      121 ttaaaagaat ctaattcttt attaagcgat ggctttgagg taaaatcggg aaaccttatt
      181 tttgataaaa agctaacgat acctttgacg acattatttg atgttattaa tccagatttt
      241 ttagcaaata gcgatagagc tgcgtatgat aattatagga cctacaaaga acagcatccc
      301 aaaaaactag ttgcattaac gtttgatgat ggtccagatc cgacgacgac tcctcaagtt
      361 ttagatattt tggcaaaata ccaggctaag ggaactttct ttatgatagg ttcaaaagtt
      421 gtgaataatg aaaaccttac taaacgtgtt agcgacgctg gccatgaaat tgctaatcat
      481 acttgggatc atcccaatct gactaatctt tcagtcagcg agattcaaca tcaagttaat
      541 atgacgaacc aggctattga aaaagcttgt ggcaagaaac ctcgctattt acgccctcca
      601 tacggagcta caaatgcaac tgttcagcaa tcctcaggat taacacaaat gctttggacg
      661 gttgatacaa gagattggga aaatcatagc actgatggca ttatgactaa tgtcaaaaac
      721 caattgcaac cgggaggagt tgttttaatg catgatattc atcaaacaac tatcaatgct
      781 ttgccgacag ttatggaata tctaaaagca gaaggatacg aatgtgtgac tgtatcagaa
      841 ctctatgcgc atcagtaagt aatatcgtta tgtttcttta gaattgatat actaaacaat
      901 ccaattactt tgaagtaatt ggattgttgt ttttagtagc ccttgattaa gttttgtttt
      961 ttaacaacta gttgatgaaa tgaagttgtt gatttttgag ggagatttca gagactttag
     1021 caaatttttt caattttttg aggtcttcag ggtggctaac aaaggtaata acgataccag
     1081 attttcccat acggccggtt cgtccagctc tgtgagtata attttcctta tcacgagcaa
     1141 catcaaaatg aatgacatag tcaagattat taatatcaat gccccgagca acaagatccg
     1201 tagctaacaa taaggagagt tggtgggatt tgaatttttc gagaatggtt ttacggaatt
     1261 tgacattgat gtcactcgct aaggaaaccg cagcagctcc attatactgg agacgttctt
     1321 ccgtagcacc caaatcagat aaactgttga aaaagaccaa agctctaaaa tgaggaatat
     1381 tagcaaattt tcgtaataaa tctgtacgtt cgcgcttgtc aaccatgagg taaaaatgct
     1441 gaatagcatc gttggtttgc tctgaaagat cgatgcagaa tgtatttgga gctaaggaag
     1501 tttgattcac cttattagta gcactcatgt ataccatttg atgatctcga ggaacatagt
     1561 ggctaatttt ttgaacaaaa tcatattggg aatcacctaa tagttcgtca tattcatcta
     1621 aaacaatggt gttgacagac atcatcttga tttttttcaa tttaatcagt tcaaaaatgc
     1681 gtccaggggt cccaatcagg atttcaggac cttttttgag gcgttcgatt tgacgttttt
     1741 gactggttcc agaaataaag agttgtgccg ttaagcctag tggctgtgcc caatcctttg
     1801 tgacttcaaa aatttggcca gctaattcgg tattaggggc taaaatgagt agttgttggg
     1861 actttttagg cgtcagtgcc aatagactag gaaagacata agctaaggtc ttgcccgtcc
     1921 cagtagggct aataccaagg aaatttttac catcaacaat aggttgaaag gcctgttctt
     1981 ggattggcgt taaatgggta aatgcaactt ggtcaagttt ttcttgccat tgagggggaa
     2041 atttagtgat catgcgtctt ccttttacta ttgtttctat attcattata gcattatagt
     2101 ctcaattttg gtaaaataat gagaaagact ttttagattt ggagtttaaa atgcttaaaa
     2161 aaccgattat tattggtgta actggtggtt caggtggcgg taaaacaagt gtgtcacgcg
     2221 ctattttgga cagttttcca aatgctcgta ttgcaatgat tcaacacgat tcttactata
     2281 aagatcagtc tcacatgagt tttgaagaaa gggtcaagac caattatgac catcctctag
     2341 cttttgatac agattttatg attcagcaac taaaagaatt attagctggt agacctgtag
     2401 atattcctat ctatgattac aaaaagcata cccgcagtaa tacgaccttt cgtcaggatc
     2461 ctcaggatgt tattattgtt gaaggtattc ttgtcctaga agatgagcgt ttgcgagatt
     2521 tgatggatat caagcttttt gtagacacag atgacgatat tcgtattatt cgtcgtatta
     2581 agcgtgacat gatggaacgt ggcagaagct tagaaagtat tattgatcag tacacaagcg
     2641 tggtaaagcc tatgtatcat cagtttattg agccaagtaa acgctatgct gatattgtta
     2701 ttccagaggg agttagtaat gtcgttgcta ttgatgttat taatagtaaa attgcgagta
     2761 ttcttggaga agtatagtgg aagtggctga gtagattatt taaatctgct cttttattgt
     2821 tgtgctcttc atagagaaag ggatcataaa atagaaatgt tattaatttg gcttggatta
     2881 ctatttgttt ggaatgaact cgtgtttgtc atatatgcct ttgataaaag aaaagccata
     2941 aaaaagaaac ggcgtattag tgaaagaaac ttattagtga taacagtttt gtttggaggt
     3001 tttggagcat tacttgcagc taaaaagtac caccataaaa ccagaaagtg gtactttgtc
     3061 atcacttggt atactagtat tttgttgaca ttactagtaa cttatatctt tatgaagtga
     3121 gtcacttaaa agacccttaa tcagtattat ttagtcaagg ctcttttata gtttaaaaaa
     3181 gcacatcatc gtcaagggtg ttgatttttt gaaaaagatc accaaagatt aaaaggatta
     3241 atgttcctag taaaactgtc atgccaggaa tgcttgttag aggagagttc cagatgaaat
     3301 gtagcaccag aggacttaat aaccagaaaa tttgttgctg ttttgaaaaa aggttagaac
     3361 cctttaataa taagtatgct ccaatggtaa aaatagctgt atgtacccaa tgtgaagtca
     3421 cagcagtgct aatacgttca atagcagtcg gtacgagact atctatagat tttgcttgaa
     3481 tcaaataacg gatatcttct gttagttgaa agcccatgcc agaaagtaaa gcaacaacta
     3541 atttttcttt aagagtaagt ctaggaaata aagtaattac taaaatagcc gaagaagcct
     3601 taatgggctc ttcgacaata ggggcagtta aagcatctcc ccaaagcttt aaagcgtctt
     3661 tagaagggat gatgtatgac caaaattgtc ctacgagatg gttcccacaa gaagctaaaa
     3721 aacctgaaat ataaagtccg ctaagacagc taacaataat aacattcagg ttcatatgaa
     3781 attttttact taaataaaaa atagctgctg caaatggaat taagtaaatg cctaatagac
     3841 taatggttag tattagagca aaagcttctt taaaggaaag gttgttagct gtcagtccta
     3901 acaattcaag ttcaatacca tttaaaacta aagcaataaa aagaccgtat cgtaaccatt
     3961 tctgttgagt gatttgtttc acgaaaacct cctaatatta agatgatatt aataatattg
     4021 aaaattatat cataaacaac ctcaaaaacc attttaaaaa ctgactgaag cgaaagatag
     4081 tcgtaaaaaa tactcgaata gacccagata tgctataata aaaggtatga ataagtctaa
     4141 aaaaattgag caataccaac taatgatagc acaggcaaaa gagctgtttg cgaatgaatc
     4201 aaatgctttg gctaatttgt caaatgccag cgccctgtta aacatgacac tacccaattc
     4261 tgtttttgcg ggattttacc tttttgatgg gcaggaatta attcttggcc cttttcaagg
     4321 tagggtttct tgcgtccata tcaagcttgg taaaggtgtt tgtggcgaat cagctcagtc
     4381 tcgtcgaacc attattataa atgatgtgaa gcagcatgcc aactatattt cctgtgatgc
     4441 ggcagccatg agtgaaattg ttgtgcctat ggtgaaagaa ggccatttga ttggggtgct
     4501 ggacttggat tctagccttg tagctgatta tgatgaggtt gatcaagaat acttagaggc
     4561 ctttgtggac ctatttcttg aaaagaccac gtttaccttt aacatgtttg gagttaaaaa
     4621 ctgatgtatc aagctcttta tcggaaatac cggagccaaa cgtttgacga aatggtggga
     4681 caatcggtta tttccacaac tttaaagcag gcagttgaat ctggcaagat tagccatgct
     4741 tatctttttt caggtcctag agggactggg aaaacaagtg cggcaaagat ttttgcaaag
     4801 gccatgaatt gtcctaacca agtcgatggt gaaccctgta atcaatgcga tatttgccga
     4861 gatatcacga atggaagctt agaagatgtg attgaaattg atgctgcctc gaataatggt
     4921 gttgatgaaa ttcgtgacat tcgagacaaa tcaacctatg cgccaagtcg tgcgacttac
     4981 aaggtttata ttattgatga ggttcacatg ttatcaacag gggcttttaa tgcgcttttg
     5041 aaaactttgg aagaaccgac agaaaatgtt gtctttatct tggcaacaac ggaattgcat
     5101 aaaattccag ccactatttt atctcgtgtg caacgctttg aattcaaagc tattaagcaa
     5161 aaagctattc gagagcattt agcctgggtt ttggacaaag aaggtattgc ctatgaggtg
     5221 gatgctttaa atctcattgc aaggcgagca gaaggaggca tgcgtgatgc tttatctatt
     5281 ttagatcagg ctttgagctt gtcaccagat aatcaggtca ccattgcaat tgccgaagaa
     5341 attacaggtt ctatttccat acttgctctg ggtgactatg ttcgatatgt ctcccaagaa
     5401 caggctacgc aagctctggc agccttagag accatttatg atagtgggaa gagcatgagc
     5461 cgctttgcga cagatttatt gacctatctg cgtgatttat tggtggttaa agctggcggc
     5521 gacaatcaac gtcagtcagc tgtttttgat accaatttgt ctctctcgat agatcgtata
     5581 ttccaaatga taacagttgt tactagtcat ctccctgaaa tcaaaaaggg aacccatcct
     5641 cggatttatg ccgaaatgat gaccatccaa ttagctcaga aagagcagat tttgtcccaa
     5701 gcaaacttgt caggagagtt aatctcagag attgaaacgc tcaaaaatga gttggcacaa
     5761 cttaaacaac aattgtcgca gctccaatcg cgtcctgatt cactggcaag atctgataaa
     5821 acgaaaccta aaaccacaag ctacagggtt gatcgggtta ccattttgaa aatcatggaa
     5881 gaaacggttc gaaatagcca acaatctcga caatatctag atgctctaaa aaatgcttgg
     5941 aatgaaattc tagataacat ttctgcccaa gacagagcct tattgatggg ctctgagcct
     6001 gtcttagcaa atagtgagaa tgcgattttg gctttcgagg ctgcctttaa tgcagaacaa
     6061 gtcatgagcc gaaataatct taatgatatg tttggtaaca ttatgagtaa agctgctggt
     6121 ttttctccca atattctggc agtaccaagg acagattttc agcatattcg taaggaattt
     6181 gctcagcaaa tgaaatcgca aaaagacagt gttcaagaag aacaagaagt agcgcttgat
     6241 attccagaag ggtttgattt tttgctcgat aaaataaata ctattgacga ctaaaactga
     6301 aatacataag ggtaaggttt tgcccttacc cacgttttat ctcgttactt ttcttcatag
     6361 tacagtattt ttttgttata ataaagctat gactattcga caatttcttt ttatggcctt
     6421 tgtatgtgcc tttgaaacct atttttttaa tgacctgttg ctctctggaa attatctctt
     6481 cgccttattt tggggcttgt tgctctttag agatttaaga agagtgcata ccattaacca
     6541 attgactaaa accattttaa aaacggctaa ttctcccaag aagaaagact gatttcaccg
     6601 cttcgcaagg tttgaaggtc accattttca aactgtacaa tgaggtatcc ttgatctgtg
     6661 agatcaatcg cgatagcacg tttttcttga ctattttcta taaaggtcac ttgtttatta
     6721 agaacaagtg atttttcttt gtaaactttg acatggtcct tgacaggaat agataagaat
     6781 aatttccaga tgtcaatgat aaggtcatta cgcgtgatag tcggtttttc ggtaaagaga
     6841 gaacctgcct tttgagcaat cgcttctggg aagtcggtga caaaaaagtt gagaccaacc
     6901 ccaataatga catcggtgat taaaccagtt tcaacagaag taatggcttc tgtcagaatc
     6961 cctgcaactt tatggttacc tagatagatg tcgtttaccc atttgatttc tgtatcaatg
     7021 cctgttaatc gcgagatagc tttgacaata ctggaagcaa ccatcatcgt ataaggaggc
     7081 atatcagcat aagggacatt gggttttagg tacatggaca tgtaaatacc accggtgcta
     7141 gctgaaaaaa attgccgatc gaggcgtcct ttggcagctt cttggctagg agctaggtaa
     7201 agccttggag cgctgttatg agcttcgatt ccttgtttgg catctaattg ggtagattgg
     7261 ctatgagggg tataggtgac tggcatccca agcccttggc taatggtttt gggtaataaa
     7321 atatcacctt gtaccatgcg gtagcccttg tgttttaaag aatcaatttg aatcccctga
     7381 ttttcgagac ttttgatgga tttccaaacg gatgtacgag agatactgag ttgatctgct
     7441 agatattccc cactgacgaa gtcatcggtt tgtgacagta attgataaat tttttctgat
     7501 gttttcatat gtatagtata ttataaaaga tcttattttc aaaggaaaag aaaagttagt
     7561 ttttttgtgt aatgctacta acaacagaaa ctattgtttg ttgaagcttc agagctccaa
     7621 ctcgtcaaca actaatagtt atcctaaaaa gagcttgagg aaaaacaagt gatgtgatat
     7681 acttaaccag ttaaataaaa aggagggttg aatgaaaacg aaaaaagtta ttattttagt
     7741 tggtctattg ttatcatctc agttgacttt gatagcttgt caatcacgag gtaatggtac
     7801 atatcccatt aaaacgaaac aatcatgtaa gggaatgacg tcaaacaaaa ttaaaccgat
     7861 taaaaaaagc aaaaagacaa acaagactca caaaggtgtg gcgggtgtcg attttcctac
     7921 agatgatggg tttattttaa ccaaagactc aaaaatctta tcaaaaacag atcagggaat
     7981 cgttgttgac catgatggtc attcgcattt tattttttat gccgatttaa agggaagtcc
     8041 atttgaatac cttattccaa aaggagcaag tttagctaag ccagctgttg ctcagcgagc
     8101 agctagtcaa gggacttcta aagtagcaga tcctcatcac cattatgaat ttaacccagc
     8161 ggatattgtg gctgaagatg ctttaggcta cacggttcgc cacgatgatc acttccatta
     8221 tattttgaag tcaagcttat caggtcagac acaggcacaa gctaaacagg ttgctactcg
     8281 cttgccacaa accagtagac ttgtttcaac agctacagct aatggtattc caggcttgca
     8341 tttcccaacc tcagatggtt ttcaatttaa cggtcaaggt attgttgggg taacaaaaga
     8401 cagtatttta gtggaccacg atggtcactt acatcctatt tcttttgcgg accttcgtca
     8461 gggtggctgg gcacatgtgg cagatcaata cgatcccgct aaaaaagcag aaaagccagc
     8521 agaaacccat cacacaccag agctatctga acgtgaaaag gaataccaag aaaaattagc
     8581 ttatttggca gaaaaattgg ggattgatcc atcaactatt aaacgtgtgg aaacacaaga
     8641 cggtaaactt ggtttggaat accctcacca tgaccacgca cacgtattga tgttatctga
     8701 tattgaaatc ggaaaagaca ttccagatcc acatgctatt gagcatgccc gtgaattgga
     8761 aaaacataag gttggaatgg ataccttgcg tgccttaggg tttgatgaag aagtgatttt
     8821 ggatatcgtt cgcactcacg atgctccaac cccattccca tcaaatgaaa aagatccgaa
     8881 taagatgaaa gactggttag caacggttat caaacttgac ttgggcagcc gtaaagatcc
     8941 tttgcaacgt aaaggacttt cactgttacc caacttagaa actttaggac ttggctttac
     9001 accaatcaaa gatatctcac ctgttttgca atttaaaaaa ttgaaacagt tgttaatgac
     9061 aaaaacaggg gtgactgatt atagattttt ggataatatg ccacagttag aaggcattga
     9121 tatttcacaa aacaatctca aagatattag tttcttgagc aaatataaaa acttaactct
     9181 agtagcggct gctgataatg gtattgaaga tattaggccg cttggtcaat taccaaatct
     9241 caaattcctc gtattgagta ataataagat ttctgattta agcccactgg catcgttaca
     9301 tcaattgcaa gaattgcaca ttgataataa tcagattaca gatttaagcc ctgtttctca
     9361 taaagaatca ttgacggttg ttgatttatc aagaaatgct gatgttgact tagcaacact
     9421 tcaagcaccc aaattagaaa cgttaatggt caatgatacc aaggtttctc atttggattt
     9481 cttgaaaaat aatcctaatc tatctagcct atctattaac cgtgcgcaat tgcaatctct
     9541 tgaaggtatt gaagcaagta gcgtcattgt cagagtagaa gcagaaggta accaaattaa
     9601 atcgcttgtg cttaaagaca agcaagggtc acttactttc ttggatgtga caggcaacca
     9661 gttgacttct ctagaaggtg ttaataattt tacagcactt gacattttaa gcgtgtctaa
     9721 aaaccaatta acaaatgtca acttatctaa acccaataag acagttacta acattgatat
     9781 tagtcataac aatatcccat tggcagacct taaattgaac gagcaacata ttccagaagc
     9841 cattgcgaaa aacttcccag cggtttacga aggttctatg gtaggtaatg gaacagctga
     9901 agaaaaagca gctatggcta ctaaggcgaa agaaagtgct caagaagcat cggaatcaca
     9961 tgactacaac cataatcata cctatgaaga tgaagaaggt catgctcacg agcacagaga
    10021 caaagatgat cacgaccatg aacatgagga tgaaaatgaa gctaaagatg agcaaaacca
    10081 tgctgactaa actaatctaa gaataacccg atccaactga atcgtcctaa tgacaccatt
    10141 gttgactatc aagagaacct caagaataac cttgaggttt ttttgaaata aataggataa
    10201 tgagtgaaaa gaatggcacc taaatggcta aaacatgtta taatagattg ttattgagtc
    10261 ccgataagac ggtctgttga acgaaatgtt tttgagacgt tgttgacttc atttggggaa
    10321 cagtcgttct ccctttagct aaattacttc gtctaaggct tattttgtgg tgacatcctc
    10381 tcaacaagga gaccggattc tttgtaactc aacgctattg tcagacattc tttgtctggt
    10441 taatggacgc tagccactag gtggactagc tagttgcctt actagtaaaa ccgtaactga
    10501 ttagctaagt tggttttggc cacttgactt gctcaatcag ctaggaagaa aggggtaggc
    10561 aacttcttgc tttgtggtca atgtgactga aaaaggaaga atcaacatat taggggggac
    10621 atttttatgt cagaacgtaa acttttcacg tctgagtccg tatctgaagg gcatccagat
    10681 aagattgcag accaaatttc agatgctatt ttagatgcca ttttagcaga agatccagaa
    10741 gcccatgtgg ctgcagaaac ttgtgtttac acaggttctg ttcatgtttt tggagaaatc
    10801 tcaacaacag catacattga tattaaccgt gtggtccgcg acaccattgc agaaattggt
    10861 tatacagaag cagaatatgg cttttcagca gaatcagtgg gagtacatcc atcacttgta
    10921 gaacaatctg gagatatcgc tcagggggtt aatgaagctc tcgaaagcag agaaggtgat
    10981 actgatgacc ttagccatat tggagcgggt gaccaagggc tcatgtttgg ttttgccatc
    11041 aatgaaacac cagaattgat gcctttacca atttccttat ctcaccaatt agtgcgtcgc
    11101 ttggcggaat tacgtaagtc aggtgagatt agttatctac gtccagatgc taaatctcaa
    11161 gtgacagttg agtacgatga acacgataaa cctgttcgtg tggatacagt cgttatttct
    11221 actcagcatg atccagaagc tactaatgac caaattcgtc aggatgtgat tgaaaaagtg
    11281 atcaaggctg taattccagc agattattta gatgatgaca caaagttctt cattaatcca
    11341 acaggacgtt ttgttattgg aggtccgcaa ggagactctg gattgaccgg acgtaagatt
    11401 attgtggata cctatggagg ctattcacgt catggtggcg gtgctttttc aggaaaagat
    11461 gcgacaaaag tggaccgttc ggcttcttac gctgctcgtt atattgctaa aaaccttgtg
    11521 gcagcaggtc ttgcgacgaa agctgaagtc caactagctt atgcgattgg ggtggctcag
    11581 cctgtgtctg ttcgcgtgga tacatttgga acctcaacgg ttcctgaagc tgttttagaa
    11641 gctgctgttc gtcaagtctt tgatttacgg ccagcaggca ttattcaaat gcttgattta
    11701 aaacgcccga tttacaaaca aacagctgcc tatggtcaca tggggagaac agatattgat
    11761 ttgccatggg agcgtttaaa taaggttgat gctttagtag aagctgtaaa aacagtatta
    11821 taaacgacaa ctttattaaa aaagtagagt gagtgaaaaa atcttactct gcttttttgt
    11881 tattaaataa gcctttaaaa gggatgatta aaaattaaga cgtcaataat tgtcaggctt
    11941 catgatttat gataaaatga aaaggttgaa ttctagtata agaaagtttt gaatgtatgc
    12001 gtaaaattat tattaatggc ggaaaagcct tatctggaga agtggcagtt tcaggggcaa
    12061 aaaacagtgt tgttgcgctt attccagcaa tcatcttagc cgatgatatt gtgattcttg
    12121 atggtgtacc tgctattagt gatgtagata gtctgattga aatcatggag cttatgggag
    12181 caacagtaaa ctatcatggt gatactttag aaattgaccc tagaggggtg caagacatcc
    12241 cgatgcctta cggaaagatt aatagtcttc gagcttccta ttatttctac ggtagtcttt
    12301 taggtcgttt tggacaagca gttgttggct tacctggtgg ttgcgattta ggaccaagac
    12361 caattgactt gcatttgaag gccttcgagg ctatgggagt tgaagtgtct tatgagggtg
    12421 aaaatatgaa tttatcgaca aatggtcaaa aaattcatgg cgctcatatc tatatggaca
    12481 ccgttagtgt cggtgctaca attaatacta tggttgcggc aacaaaagct caaggtaaaa
    12541 cggttattga aaacgctgca cgtgagccag agattattga tgtggctact cttttgaata
    12601 acatgggtgc gcatattcgt ggagcaggta cagatattat tacgatacaa ggtgttcaga
    12661 aattgcatgg tactagacac caagttattc ctgatcgtat tgaagcaggg acttatatcg
    12721 ctttggcggc agctattgga aaaggtgtta agatcactaa tgttctttat gagcatttag
    12781 agagtttcat tgctaagtta gaagaaatgg gtgtccgtat gacggtagaa gaggatgcta
    12841 tctttgttga aaagcaagaa tccctcaagg ctattacaat taaaacctct ccttaccctg
    12901 gttttgccac tgatttgcaa cagccattaa cgcccttgtt gttaaaagca gatggtcgtg
    12961 gaacgattat tgacactata tatgaaaagc gcattaacca tgtgccagag ttgatgcgca
    13021 tgggagcaga tatctcagtt attggtggtc agattgttta tcaagggccg tctcgcctga
    13081 caggagctca ggtcaaagcc actgatttac gtgccggagc ggctctagtg actgctggtc
    13141 taatagctga aggcaagaca gaaattacta atattgaatt tatcttacgt ggctatgcta
    13201 gtatcattgc aaaattaaca gcactagggg cagatattca attaatcgaa gactaaagag
    13261 aagctaacgc ttctcttttt tatattacat gtgcttaggc taatgatact taggtgcata
    13321 ctttgttgca ttttatggct ttttaactgt cataaaaaag ataaaaatga tatcactaac
    13381 taatattatt aattttaatg ctaaaataat gagttgacgg tagtaaagtg ataaataaac
    13441 actccttaaa atattatttt attaattttt taaaaaaata atatataaat tagataataa
    13501 acttgat
//
LOCUS       DAUCWO010000045        13165 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUCWO010000045 DAUCWO010000000
VERSION     DAUCWO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.045"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            30..785
                     /gene="recO"
                     /locus_tag="VGY20_001252"
     CDS             30..785
                     /gene="recO"
                     /locus_tag="VGY20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ4274103.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAVI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            888..1895
                     /gene="plsX"
                     /locus_tag="VGY20_001253"
     CDS             888..1895
                     /gene="plsX"
                     /locus_tag="VGY20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ4274104.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDSLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            1888..2130
                     /locus_tag="VGY20_001254"
     CDS             1888..2130
                     /locus_tag="VGY20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ4274105.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            2281..2985
                     /locus_tag="VGY20_001255"
     CDS             2281..2985
                     /locus_tag="VGY20_001255"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ4274106.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            3109..6834
                     /locus_tag="VGY20_001256"
     CDS             3109..6834
                     /locus_tag="VGY20_001256"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ4274107.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYQADDFATYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDDVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGEVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDVKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKDNFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGAGLLAAFEGKLEEVYPTEFEQADALEEVPAVVSDAVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGSSVNLVPFVTLNEAAITESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIENGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            6995..8449
                     /locus_tag="VGY20_001257"
     CDS             6995..8449
                     /locus_tag="VGY20_001257"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ4274108.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPNFIGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCNRYTDETQLAICSMEYIYFARPDSTIHGVNVHTARKNMGK
                     HLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            8477..9499
                     /locus_tag="VGY20_001258"
     CDS             8477..9499
                     /locus_tag="VGY20_001258"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ4274109.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            9667..10221
                     /gene="purN"
                     /locus_tag="VGY20_001259"
     CDS             9667..10221
                     /gene="purN"
                     /locus_tag="VGY20_001259"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ4274110.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            10405..11952
                     /gene="purH"
                     /locus_tag="VGY20_001260"
     CDS             10405..11952
                     /gene="purH"
                     /locus_tag="VGY20_001260"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ4274111.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVADPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDVDAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(12011..13135)
                     /locus_tag="VGY20_001261"
     CDS             complement(12011..13135)
                     /locus_tag="VGY20_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ4274112.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNENTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
BASE COUNT         3903 a         2426 c         2971 g         3865 t
ORIGIN      
        1 aaggtaagag aaaaatacga cgaggtaata tgcaactaac agaatcacta ggcattgttc
       61 tttttaatag gaattatcga gaagatgata aattagtcaa aatatttact gaagtagcag
      121 gtaagcagat gtttttcgtg aaacatatta gtcgttccaa aatgtcctca atcattcaac
      181 cactaacgat tgctgatttt attttcaagt taaatgatac aggcttgtct tatgttgttg
      241 actatagtaa cgttaacact tatcggtata ttaataatga tatttttcga ttagcctatg
      301 ctagttatgt cttagcatta gctgatgctg tgattgcaga taatgaatca gattcgcatt
      361 tgtttacgtt tttgaaaaaa acacttgatt tgatggaaga gggcctagat tatgaaattt
      421 tgacaaatat ttttgaaatt cagatattag atcgttttgg tattagtcta aactttcatg
      481 agtgtgccat ttgtcatcgc actgatttac cgcttgattt ttcccatcgt ttttcagctg
      541 tactttgttc tgaacattat tacaaagaca accgacgtaa tcatttagat ccaaatgtta
      601 tctacttgtt gagtcgattt caaaaaatca catttgatga tttgagaact atttcattga
      661 ataaagacat caaaaagaag cttcgtcagt tcattgatga gttgtatcac gactatgtag
      721 gaatcaaatt aaaaagtaaa acattcattg ataatttagt taagtgggga gatattatga
      781 aatgataggg cctctttcgt ttatgggaat tggtttcaga atatgctata ataaaaatac
      841 tattaataga ttgtgtaaga acacaagata taattaaagg agtggaaatg aagagaattg
      901 caatagatgc tatgggtgga gataacgctc ctaaagctat tgttgagggt gttaatcaag
      961 caatagaagc attttcagat atcgaaattc aactttacgg tgatcagaca aagattaact
     1021 cctatcttat tcaatcagat cgtgttgcta ttattcatac agatgaaaaa attatgtcag
     1081 atgatgaacc tgcaaaagct gttcgccgta aaaagaaggc ttcaatggtt ttagcagcta
     1141 aggcagtgaa agaaggaaaa gcagatgcca ttatttcagc agggaataca ggtgctcttt
     1201 tagctgtagg tttgtttgtt gtggggcgta tcaagggagt tgatcgtcca ggattattat
     1261 caactattcc cacagtaacc ggattaggat ttgatatgtt ggatcttgga gctaatgctg
     1321 aaaatacggc aaaacattta catcaatatg ctattttagg ctctttctat gctaagaatg
     1381 tacgtgggat tgctaatcca cgtgttggtc tattaaacaa tggtacagaa gaaacaaaag
     1441 gagattccct tcgtaaggct acttatgaac ttttaacagc agataataca atatcttttg
     1501 ttggtaatgt cgaggcgcga gaacttatgt caggtgttgc agatgttatt gtatcagatg
     1561 gtttcacggg aaacgcagtg ttgaaatcaa tagaaggaac agctattagc attatggggc
     1621 aactgaaaca gataattaat agcggcggta ttaagactaa aattggagct agtctactta
     1681 aaagtagtct ttacgagatg aagaagactc ttgactactc tagtgctgga ggtgcagttt
     1741 tatttggttt aaaggcacct gttgtaaagt ctcatggttc tagtgatgta aaagctattt
     1801 tttcaacgat aaaacaagta cgtacgatgc tagacactaa cgtggtcgga caattagtag
     1861 aagaatttgc taaggagact caagtaaatg actagacagg agatttttga aaggctgatt
     1921 aacttaattc agaagcaaag aagctatttg agtgtagcta ttacagaaca aacacattta
     1981 aaaaatgatt tagcggttga ctctattgaa ctagttgaat ttattattaa tgttgaagat
     2041 gaatttcata ttgctattcc tgatgaagat gttgaggata tggtatttat gggagacgtt
     2101 ttagattatt tagttcagcg tttgacttag ttagaataat aaaaagagcg taagctcttt
     2161 ttattattct ttaaaaacga accttttttt aaaaaaactt ataaacgtaa ataatatctt
     2221 gaatattttt atagagtatg ttactcttaa atgaaaacag aaagaaaggc gaataatcaa
     2281 gtgactaacc agcttattta taaaggaaaa gcgaaagata tttatagtac aaaagatgaa
     2341 aatgtgatta gaacggtcta taaagaccaa gctactatgt tgaatggagc tcgtaaggag
     2401 actattgatg gcaagggagc actaaataat cagatttcat ctcttatttt tgagaaatta
     2461 aataaggctg gtgtggtgac acattatatt gagcaaattt caaaaaacga acaattgaat
     2521 aaaaaagttg atattattcc acttgaggtg gtgttacgga atgtgatagc aggctcattt
     2581 tcaaaacgtt ttggagtgga agaaggacgt gttcttgaaa cgccaattgt ggaattttac
     2641 tacaaaaatg atgatctgaa tgatcctttt atcaatgatg agcatgtgaa gtttttgggt
     2701 attgttaatg atgaggagat tgcttatctg aaaggagaga ctcgccgtat taatgaactc
     2761 ttgaaaggtt ggtttgctca gattgggctt aacttgattg acttcaagtt ggagttcgga
     2821 tttgatcaag aaggtacaat tatcttagca gatgaatttt cgcctgataa ctgtcgccta
     2881 tgggataaga atggtaacca tatggacaag gatgttttta gaagagacct tggcaacctg
     2941 acggatgtct atcaagttgt acttgagaag ttgatagcat tgtaactaga agaggaagga
     3001 attgaatccg ttcgggaaac tccgaaaaaa agatggaact tactgaaatc tcaatttcag
     3061 atgtcctcct attttccagt cgctcccttg tcggacttgg tatcttatat gaataaacgt
     3121 atttttgttg agaaaaaggc tgactttggt attaaatcgg ctagtcttgt gaaagagttg
     3181 acgcataatc tacaactgac ctctttgaag gctttgcgta ttgtgcaggt ctatgatgtc
     3241 ttcaatttgg ctgaggattt gctggcgcgt gctgagaagc atattttctc tgagcaggtg
     3301 acagactgtc ttttgacgga aactgaaatc actgcggagc ttgataaggt tgccttcttt
     3361 gccattgagg cgcttcctgg tcaatttgac caacgtgctg ctagttcgca agaagctttg
     3421 ctattatttg gaagtgacag tcaggttaag gtcaatacag cccagcttta cttggtcaat
     3481 aaggatatta cagaagcaga gcttgaagcc gttaagaact atcttttgaa ccctgttgat
     3541 tcgcgtttca aggacattac tttgccgctt gaagagcagg ctttctctgt gtctgataag
     3601 acgatcccta atcttgattt ctttgaaact tatcaagctg acgattttgc gacttataag
     3661 gcagagcagg gcttggctat ggaggtcgat gaccttctct tcatccaaga ttatttcaaa
     3721 tcaatcggac gtgtgccaac tgagactgag ttgaaagttt tggatactta ctggtcagac
     3781 cactgccgtc acacaacctt tgaaactgaa ttgaagaata ttgatttttc agcttctaaa
     3841 ttccaaaaac aattgcagac gacttatgac aaatatatcg ccatgcgtga tgagcttggc
     3901 cgttctgaaa agccacaaac acttatggat atggcgacta tttttggtcg ttatgagcgt
     3961 gccaacggtc gtctggacga tatggaagtc tcagatgaaa tcaatgcctg ctcagttgag
     4021 attgaagtag atgttgatga tgtgaaagag ccttggctcc tcatgtttaa gaacgagact
     4081 cacaatcacc caacagaaat tgagccattc ggtggagcgg cgacttgtat cggtggtgct
     4141 attcgtgacc cattgtcagg acgttcatac gtttatcagg ctatgcgtat ttcaggcgca
     4201 ggcgatatca cgactccgat tgcggaaaca cgtgctggta aattgccaca acaagttatt
     4261 tctaaaactg cggcgcacgg ctattcttca tatggtaacc aaattggact tgcgacaact
     4321 tatgtgcgtg agtacttcca ccctggcttc gtagccaaac gcatggagct tggagctgtg
     4381 gtcggagcgg cacctaagga aaatgtggtt cgtgaaaaac cagaagcagg tgatgtggta
     4441 atcttgcttg gtggtaaaac agggcgtgac ggtgtcggcg gtgcgacagg ttcatctaag
     4501 gttcaaacgg ttgagtctgt ggaaacagct ggcgcagagg tacaaaaagg gaatgccatc
     4561 gaagagcgta agattcaacg tctcttccgt gatggtgaag tgactcgtct catcaaaaaa
     4621 tcaaacgact ttggtgctgg tggtgtctgt gttgccatcg gtgaattggc tgacggtctt
     4681 gaaattgatt tggacaaggt gcctcttaaa taccaaggtc ttaatggtac tgaaattgca
     4741 atctcagaat ctcaagagcg tatgtcagtc gttgttcgtc caagtgatgt ggatgccttc
     4801 atcgcagcct gcaacaagga aaatatcgat gcagtcgttg ttgcgaccgt tactgaaaaa
     4861 ccaaatcttg tcatgacttg gaatggcgaa atcatcgttg atttggaacg ccgtttcctt
     4921 gataccaatg gtgtccgtgt tgttgttgat gttaaagtcg ttgacaagga cttgacagtt
     4981 ccagaagcac gcacaacatc agcagagaca cttgaagcag atacgcttaa ggtcttgtct
     5041 gacctcaacc acgctagtca aaaaggtctt caaactatct ttgactcatc tgttggtcgt
     5101 tcaaccgtta accacccaat cggtggtcgt taccaaatca caccgacaga aagttctgtt
     5161 caaaaattgc cagttcaaca tggtgtgaca acaactgcat ctgttatggc tcaaggttac
     5221 aatccttata ttgcagagtg gtcaccttat cacggtgctg cctatgctgt cattgaagcg
     5281 acagctcgct tggtagcaac gggtgctgac tggtctcgtg cacgtttctc ttaccaagag
     5341 tactttgagc gtatggataa acaggcagag cgttttggtc agccagtatc agctcttctt
     5401 ggttctattg aggctcagat tcaacttggt ttgccatcaa tcggcggtaa ggactctatg
     5461 tctggtactt tcgaagactt gacagtacca ccaaccttgg tagctttcgg cgtgacaaca
     5521 gcggacagcc gcaaggttct ctctcctgag tttaaagcgg ctggcgaaaa catttactat
     5581 atcccaggtc aagctatttc agaagatatt gattttgacc ttatcaagga taactttagc
     5641 cagtttgaag ctattcaagc tcaacataag attacagctg cctcagccgc taaatacggt
     5701 ggtgtcctag aaagtcttgc tctcatgact tttggtaacc gtatcggtgc ttctgttgaa
     5761 attgcagagc ttgacagcag cttgacagct caactcggag gttttgtctt tacatcagct
     5821 gaggaaattg ctgacgcggt gaaaatcggt caaactcagg cagactttac agtcactgtc
     5881 aatggaaatg accttgctgg cgctggcctt ctagcagcct tcgaaggcaa attggaagag
     5941 gtttacccaa cagaattcga acaggcagac gctcttgaag aagttcctgc tgtggtatca
     6001 gatgctgtta tcaaggctaa ggaaacaatt gaaaaaccgg tggtttacat tccagtcttc
     6061 cctggtacca actcagagta cgattcagct aaggcctttg aacaggttgg atctagcgtc
     6121 aacttagtac catttgtaac cttgaatgag gctgctatta ctgaatcagt tgacactatg
     6181 gttgctaata ttgctaaggc aaatatcatc ttctttgctg gaggtttctc agcagcagat
     6241 gaaccagatg gctctgctaa gtttatcgtc aatatcttgc ttaacgagaa ggtgcgcgca
     6301 gctattgaca gcttcatcga aaatggaggc cttatcatcg gtatctgtaa tggtttccaa
     6361 gcccttgtta aatcaggtct tcttccatac ggaaacttcg aagaagctgg tgagacaagt
     6421 ccaactctct tctataacga tgcgaaccaa cacgttgcca agatggttga gactcgtatc
     6481 gcaaatacca actcaccttg gttggcagga gttgaggtcg gcgatattca tgccattcca
     6541 gtttcacatg gtgaaggtaa atttgttgtc agcgcttctg aatttgcaga gctaagagac
     6601 aatggtcaaa tctggagcca atatgtggac tttgacggac aaccatctat ggattctaaa
     6661 tacaatccaa acggctctgt caatgccatc gaagggatta ccagcaagaa tggtcaaatc
     6721 atcggtaaga tgggacactc agaacgctgg gaagacggac tcttccaaaa tatccctggt
     6781 aacaaagacc aaaccctctt tgcaagtgct gtaaaatact ttacagggaa gtaaaaaggt
     6841 tcagtgaacc tttttagccc tcgccttgaa ttagtgaagg gcgaggttag aaagaatctc
     6901 gcttaatttt tgagaaatag taaacacggg cgataagttg tgcgaaaaac tttctatttt
     6961 cgctttactt gacagccttt gtatcgaggt aaaaatgaca tacgaagtaa aatctctaaa
     7021 tgaagaatgt ggagtctttg gtatctgggg acatcctcag gcagctcaag tcacttactt
     7081 tgggcttcat agccttcaac atcgcggtca agaaggagct ggtattgttt caaatgacaa
     7141 tgggaaactc tatggttatc gaaatgttgg gctcctttct gaagttttta agaatcaatc
     7201 tgaattagat aatttaactg ggaatgcggc tattggacat gttcggtatg ctactgcagg
     7261 ttctgcagat attcgcaata ttcagccttt tctttataaa tttcatgacg ggcaatttgc
     7321 tttatgccat aatggtaatt tgacaaatgc tatttcctta aggaaagaat tagaaaagca
     7381 aggtgcaatt ttcaatgcct cctcagatac tgaaatttta atgcatttga ttcgtcgaag
     7441 ccataaccca aatttcatag gaaaggtaaa agaagctcta aatacagtaa agggtggctt
     7501 tgcctatcta ctgatgacag aaaataaact cattgctgct cttgacccta atgcctttcg
     7561 tcctttgtca attggacaaa tgcaaaatgg tgcctgggtt atttccagtg agacctgtgc
     7621 ttttgaggtg gtaggcgcaa aatgggttag agatgttgaa cctggcgaag ttattcttat
     7681 agacgatagt ggtattcaat gtaatcgtta tactgatgaa acgcaactgg caatttgttc
     7741 gatggaatat atctattttg caaggccgga ttcaactata catggtgtta atgttcatac
     7801 ggctcgaaaa aatatgggaa agcatcttgc acaagaattt aaacaggatg ctgatattgt
     7861 aattggtgtc ccaaattcgt ccttatcggc tgctatgggc tttgctgaag aatccggatt
     7921 accaaatgag atgggtcttg taaaaaatca gtatacgcag cgaaccttta ttcaaccgac
     7981 acaagaatta agggaacaag gtgttcgaat gaaactatca gcggtatctg gtgttgtcaa
     8041 aggaaagcgc gttgttatga ttgatgactc aattgtaaga ggaacgactt ctagaaggat
     8101 tgtcggatta ttaagagaag caggagctag tgaagtacat gttgctatag ctagtccaga
     8161 attaaagtat ccttgttttt atggtattga tattcaaaca agacgagaat taatttcagc
     8221 aaatcacgct gctgacgaag tgtgtgatat tattggcgca gatagtctga cctatctctc
     8281 actagatggg ttgattgaat caattggact cgaaacaaaa gcaccaaatg gtggtttatg
     8341 cgtagcttac tttgatggac attatccaac accgctttat gattatgaag aagaatacct
     8401 cagaagttta gaagaaaaaa caagttttta tattcaaaaa gtgaaataag ctctgctaat
     8461 tatataagga gaaaacatgt ctgaaaaaaa tgcttatgca aaatctggtg ttgatgttga
     8521 agctggttat gaagtggttg aaagaataaa aaaacacgtt gctcgtacag aacgtgcggg
     8581 agtcatggga gctctaggtg gctttggtgg gatgtttgac ttgagtaaaa caggagttag
     8641 agagcctgtt ttggtctcag ggacagacgg tgttggcaca aaactcatgc ttgctatcaa
     8701 gtacgacaag cacgacacaa tcggtcaaga ctgtgttgcc atgtgtgtca atgatattat
     8761 tgcagcaggt gctgagcccc tttacttcct tgactacgta gcgacaggaa aaaataatcc
     8821 agtcaaattt gaagaagttg tttctggcgt tgcagaaggt tgtgttcaag caggagcagc
     8881 actcattgga ggtgagaccg ctgaaatgcc tgggatgtat ggcgaagatg attatgacct
     8941 tgctggcttt gctgttggtg tggctgaaaa atctcagctc atcgacggtt caaaagtaaa
     9001 ggaaggcgat attcttttgg gacttgcttc aagtggtatc cattcaaacg gttattcttt
     9061 ggtacgtcgt gtctttgctg actatactgg taaagagctg cttccagaac ttgaaggaaa
     9121 acaacttaag gatgtccttc ttgagccaac tcgtatttat gttagagcag ctctgccatt
     9181 aatcaaggaa gaactagtca acggtatcgg ccacatcaca ggtggtggct ttatcgagaa
     9241 tgttcctcgt atgtttgcag atgatttggc tgcggaaatc gatgaggaca aggtgccagt
     9301 gcttccgatt ttcaaggcgc ttgaaaaata tggtgacatc aagcacgaag aaatgtttga
     9361 aatcttcaat atgggtgtcg gtcttatgct agctgttagt cctgaaaatg ttaaccgcgt
     9421 caaagagctc ttggatgaac cagtttatga aatcggtcgt atcatcaaga aagcagacga
     9481 tagcgtggtg attaaataag tcactaaatt ttataagaaa ggagtgttta gttagtttgc
     9541 ctaactgaac acgggattaa atttctagga aaaaagataa aatgacttag acgcaagcgt
     9601 cgttgttcgt tttcctattt ttctacgaaa tttttcggca agccgaatca tccctttgta
     9661 tcgaatatga aaatcgctgt ttttgcttct ggtaatggtt ccaactttca ggtcatagca
     9721 gagcagtttc cagttagttt tgtcttttca gatcatcgtg atgcctatgt tttagagcgt
     9781 gctcagaatt tagccattcc aagcttcgct tttgaactca aggagtttga gaataaggcg
     9841 gcttacgagc aagccatcgt tgatttgttg gacaaacacg agattgattt ggtctgtcta
     9901 acgggctata tgaagattgt tggagaaacc ttactcttgg cctatgaggg gcgtattatc
     9961 aatattcacc cagcctacct gcctgaattt ccaggtgccc acggtatcga ggatgcttgg
    10021 gaagctggtg ttgaccagtc tggcgtgacc atccactggg ttgactctgg tgtggatacc
    10081 ggtcaggtca tccaacaagt gcgcgtgcca cgcctagcag acgatagcct agaaagcttt
    10141 gaaacccgta tccatgaaac cgaatatcaa ctctacccag ctgtcttaga tagcttggga
    10201 gtggagagga aagtaatcta gcctttatct taaaatttta ggtaaagtta ggaaagatag
    10261 taaagaagaa ttaacgttcc aaaaagaaag gaattgaact cgtctggaag gcacagaaaa
    10321 aagataaaca tctcctaggc gcaagcgtct tcgtcgagtt tcctattttt ccttggcctt
    10381 ctagtcggac ttggtatctt aaatatgact aaacgtgctt taatctcagt gtctgacaag
    10441 tcaggaattg ttgactttgc aaaagaattg aaaaacttgg gttgggatat tatctcaact
    10501 ggtgggacta aggttgccct tgacgatgct ggtgttgaga ccattgccat cgacgatgtg
    10561 actggattcc cagagatgat ggacggtcgt gttaagaccc tccacccaaa cattcacggt
    10621 gggcttttgg ctcgtcgcga cgctgacagc caccttcagg ctgctaagga caacaatatc
    10681 gagctgattg acctcgtggt tgtcaacctc tatcccttca aggagactat ccttcgtcca
    10741 gacataacct atgatttggc ggtggaaaac atcgacatcg gcggtccatc aatgcttcgc
    10801 tcagccgcta aaaatcacgc tagcgtgacc gttgtggctg acccagcgga ctatgcgact
    10861 gttttgggag aattggctga cgctggtcag acgacatttg aaacgcgtca acgcttggca
    10921 gctaaggtct ttcgtcacac agcagcctac gacgctttga ttgctgagta cttcacaact
    10981 caagtgggag aggctaagcc tgaaaaatta accatcactt acgaccttaa acaggctatg
    11041 cgttacggag aaaatccaca acaagacgct gatttctatc aaaaagcctt gccaacagac
    11101 tactcaattg cttctgctaa acagctcaac ggaaaagaat tgtccttcaa taatatccgt
    11161 gatgttgatg cagcaatccg tattatccgt gatttcaaag accgtccgac agtggttgcc
    11221 ctcaaacaca tgaacccatg tggtatcgga caggctgatg atattgagac tgcttgggat
    11281 tacgcttatg aagctgaccc agtttcaatc tttggtggaa ttgtcgtcct taaccgtgaa
    11341 gttgacgcag cgacagccaa gaagatgcac cctatctttt tggaaatcat catcgcacca
    11401 tcatactcag aagaagcgct agctattctc acaaataaaa agaaaaactt gcgaatcctt
    11461 gagttgccgt ttgatgccca agctgccagc gaagtggaag ctgagtacac tggcgtagtt
    11521 ggtggacttt tggtgcaaaa ccaagacgtt gtggctgaaa atccatctga ctggcaagtg
    11581 gtgacagacc gccaaccaac agaacaagag gcgactgccc ttgagtttgc ctggaaggct
    11641 atcaagtatg ttaagtctaa cgggattatt attactaacg atcacatgac gcttggactc
    11701 ggtgcaggtc aaaccaaccg tgtcggctca gtcaagattg ctatcgagca ggctaaagac
    11761 caccttgacg gtgccgttct agcatcagat gccttcttcc catttgcgga caacattgaa
    11821 gaaatcgctg ccgcagggat caaagcaatc atccaaccag gtggttcagt tcgtgaccaa
    11881 gactctattg acgccgcaaa caaacatggc ttgaccatga tcttcacagg tgtgagacat
    11941 tttaggcatt aataaaaaaa tgtggtctaa ttaaaaccac atttttttat taaaagaaaa
    12001 tataagttaa ctataatttg ttagctttat cagcatttac ccatgttcca cgagatccga
    12061 tttgaacata aataccatta cttggttgat caatttttaa tactttataa ctaccagtga
    12121 cggtaaaata ttcacccaca cggagaattt gatctcctaa aacttttcct tggttatctg
    12181 tttcatctaa tggtgtggga tcaatccagt ttagtggagt tgggtctcct ccggctaatt
    12241 ctttattaac gatcaaatta ttaacaagct gatctacacg aaaaacgcca gggaaggtaa
    12301 cagtatcacc gaccttaatg gaaggttttg tattttcatt ttgaaccaca gactgtgcgg
    12361 gctctttaat gggaatatag cgtcggtttc cagaaaaact gaggtagcta agccatgtat
    12421 aacctccagc agtcacgact ttatcgtaat aaacagattg acctgcttca tagtaagcaa
    12481 gatcagggct atcgatactg gtttgtcctt tgactggtaa tctggttgta aagtgataag
    12541 ttccagaggg gtcaaatgaa gcttgagaaa tgtgttttag ttgtcttggg taggaatgac
    12601 ttgtctgaga tgataagtct ttaaaatgga tataaccact tacctgagat tttggaattt
    12661 gacgcttatg gtatctttca gggccttgtc cagcgttgta attatactct tcgatagtga
    12721 cagtgtctcc acggatatca gctacccatg ctacatgacc gtaagcagca tttgactgat
    12781 aagcgttttt atcaaaccaa gcgatagccc ctatgcttgg tgtcttattc acaggataac
    12841 cctgattttt cgcgatatgt ccccacgtgc aggcattacc gtagccttta ggtaactgaa
    12901 aaccattagc agagcttaaa cgaaaagctg caaaagaagt gcattggcgg atatacatgt
    12961 tccacgaatc gattccattg ccttttttcc acttactcgg atagttatcg cctaaaacag
    13021 ctgcatgaac aagattttcc tgttgcgaaa taagtgtaga tggcatagta ggtactagac
    13081 cattaaaacc taaaaggatt actcctgaga ccaaaaaacg atgaaatttt ttcattaatt
    13141 acctcctgta acttattatt cgaaa
//
LOCUS       DAUCWO010000046        13139 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUCWO010000046 DAUCWO010000000
VERSION     DAUCWO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.046"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            26..1360
                     /gene="brnQ"
                     /locus_tag="VGY20_001262"
     CDS             26..1360
                     /gene="brnQ"
                     /locus_tag="VGY20_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ4274113.1"
                     /translation="MLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLG
                     VIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGATSFSVGIAPILG
                     DNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISILVITSFVHPAGNY
                     GDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVT
                     KITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRF
                     YLGGIGQSFLAVVIFLACLTTSTGLITSSAEYFHKLVPTLSHIAWATIFTLLSAFFYF
                     GGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIFDALL
                     TLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS"
     gene            complement(1414..2106)
                     /locus_tag="VGY20_001263"
     CDS             complement(1414..2106)
                     /locus_tag="VGY20_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274114.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(2107..3171)
                     /locus_tag="VGY20_001264"
     CDS             complement(2107..3171)
                     /locus_tag="VGY20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274115.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFEQGKVQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            complement(3421..4266)
                     /locus_tag="VGY20_001265"
     CDS             complement(3421..4266)
                     /locus_tag="VGY20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ4274116.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            complement(4593..5435)
                     /locus_tag="VGY20_001266"
     CDS             complement(4593..5435)
                     /locus_tag="VGY20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4274117.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            complement(5638..6354)
                     /locus_tag="VGY20_001267"
     CDS             complement(5638..6354)
                     /locus_tag="VGY20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4274118.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(6837..7943)
                     /locus_tag="VGY20_001268"
     CDS             complement(6837..7943)
                     /locus_tag="VGY20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ4274119.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            complement(8164..8907)
                     /locus_tag="VGY20_001269"
     CDS             complement(8164..8907)
                     /locus_tag="VGY20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4274120.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKSRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEVPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTETSKRWFFVAYK"
     gene            complement(8955..9308)
                     /gene="rsfS"
                     /locus_tag="VGY20_001270"
     CDS             complement(8955..9308)
                     /gene="rsfS"
                     /locus_tag="VGY20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ4274121.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            complement(9332..9925)
                     /gene="yqeK"
                     /locus_tag="VGY20_001271"
     CDS             complement(9332..9925)
                     /gene="yqeK"
                     /locus_tag="VGY20_001271"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ4274122.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            complement(9922..10554)
                     /locus_tag="VGY20_001272"
     CDS             complement(9922..10554)
                     /locus_tag="VGY20_001272"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ4274123.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDYYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            complement(10623..10931)
                     /gene="yhbY"
                     /locus_tag="VGY20_001273"
     CDS             complement(10623..10931)
                     /gene="yhbY"
                     /locus_tag="VGY20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ4274124.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            complement(10956..12074)
                     /gene="yqeH"
                     /locus_tag="VGY20_001274"
     CDS             complement(10956..12074)
                     /gene="yqeH"
                     /locus_tag="VGY20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ4274125.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETEELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIGRINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLARFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPDKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            complement(12074..12601)
                     /locus_tag="VGY20_001275"
     CDS             complement(12074..12601)
                     /locus_tag="VGY20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ4274126.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
BASE COUNT         4005 a         2756 c         2063 g         4315 t
ORIGIN      
        1 aaatgtatat atcgtcattg gattcatgct atttgcacta ttttttggag cagcaaacct
       61 catttaccca gcttttttag gcatctactc cggtcatcag attctatggt ctatcattgg
      121 tttttgttta actggtgtct ccttgccttt actcggtgtc attgctgttg ctaaatctgg
      181 atcaggcgat gttgaaagtt tggcacgccc catctctaaa tggtatgcaa tcttctattc
      241 ttccattttg tatttatcta ttggtccatt ttttgctatt ccaagaacag gagccacttc
      301 tttttcagtc ggtatcgctc ctatcttagg agataataca accaataaag ctatttatgc
      361 tatattattt tttggtctgt cctacttcct tgctatcaaa cctagtaagc tagctgaaaa
      421 tatcggaaaa tttttaacgc caacgttgtt agttgttatt tctattttgg ttatcacgtc
      481 ctttgtccat cctgctggaa attacggtga tgcttttaac gctggggtcg gtgttaataa
      541 tgcctttaaa gattttcctt ttatagcagg attaattcaa ggttatggca ctatggatgc
      601 actagcttct cttgtttttg ctattttagt cattgaggct accaaacaat ttggcgctaa
      661 gacggacaaa gaagtgacca aaataacact tatttctggg gctattgcca ttttgctatt
      721 agcacttgtc tatatctttg tcggtcgtat tggagcaaca tcacaatcat tatttccttt
      781 tagtgatggc agctttaccc ttcatggtaa tccagttaat ggcggtcaaa tcttaagtca
      841 tgcttctcgt ttttacctag gtggcatcgg acaatcattt ctagctgttg tgattttcct
      901 ggcctgtcta accacttcaa caggcttaat cacgtcaagt gctgaatact tccataaatt
      961 agtgcctact ttatctcata ttgcttgggc aactatcttt actttactat cagctttctt
     1021 ttattttggt ggcttatcag tcattatcaa ctggtcagct cctgttttat tccttttata
     1081 cccattaaca gtcgatttaa ttttccttgt tttggcacaa aaatgcttca ataatgatcc
     1141 tattgtctat cgaactacaa ttggtctaac ctttattcct gccatatttg atgcactcct
     1201 aacactatca caaatgactg gattatttca tttaccagaa gccgttgtaa cttttttcca
     1261 aaaaactgtt ccactagggc aattctcaat gggatggatt atctttgctg ctattggttt
     1321 tttaataggg cttatactaa gtaaaacgaa gaaaagctaa tgacatcatc taaacaacaa
     1381 caccctaagt aaaaacttag ggtgttgttt cgtttattta tgactaaacc ttctagtcaa
     1441 gctatcacca ataaattgga tagcaaaaat aattaaaaga ataataatag tcgctaccca
     1501 agtcacatca ttgttaaaac gattataacc ataagaaatc gctacatttc ctaatcctcc
     1561 agcaccgata gctcctgcca tggcagtttc cccaactaaa gaaattaagg tgactgtaga
     1621 aacacgaata agatctggta aaccttcact gagatacact ttgacaatat cccagaaagt
     1681 agcacctgaa gcttgcgctg cttcaataac acccttgtct aattctgaaa aaacaacttg
     1741 gacctgacgg gcataaaaag gaaaagtcgc aaaggttaaa ggtaccaagg ccgctgttgc
     1801 ccctaaagtt gttcttaaaa gcaaataggt aaagctagct aaaatagcaa tcaaaataac
     1861 aaaaggaatg gctctaaaaa tagaggtaac tttatcaata acccaacaaa tcgttttgtt
     1921 ttcaataaca ccgtccggtc ctgttaaaac taatagtaag cctaataaga gaccaatagc
     1981 acccccaaca ataaaaggca caatggtcat gtataaagta ttccaaatcg ctaagcccca
     2041 accagcatct ccagaccatc cgagttcata aacatttggc aaataagttt gaattaactg
     2101 tgacatctag gctcctctct ttaaaatact aacatcaaca ccagcttcat gcaaggcttt
     2161 ctcagccgca agaatatttt cagcttgccc ttccaagaca acaatcatgt caccaactgg
     2221 aatatgatct aaaatctcaa tattgccata caaaatatta gctgtcactt caaattgacg
     2281 gtaaatgcta ttaagtagag gctcatccgt agaagttcct gcatatttca actgagctag
     2341 aagggcattt gcaggcaaat gtttaacaat gtcttgttgg ttaatttttt ctaaagcctc
     2401 atcaatccct gtagctgttg tgataaattc ctgtgtcaag gcttcttttg gatttgaaaa
     2461 gatatcaagg acagaacctt cttcaatcaa aacaccattt tgcataacag caacacgatt
     2521 acaaatatcc ttaacaattt gcatttcatg agtaatcata acaatggtaa ggccaagttt
     2581 gcggtttaat tcttgcaaga gcgccaaaat ttgtttagtg gtttttggat ctagtgctga
     2641 tgtggcctca tctgaaatca aaatttttgg atcgttagct agagcacgcg caatagccac
     2701 cctttgtttt tgcccacccg ataactgggc agggtagtta tctgctcgct ctgataaacc
     2761 tacaagttct agcagctcga taactttatg ttctttctca gtcttactta acgatgaatg
     2821 gcggagcgca aatgctacat tttctttggc tgttttttgg gccatcaaat tgaaatgttg
     2881 gaagatcatg ccaatatcac gtcgtttttg acgaagggca ttggctgata actgaacttt
     2941 cccctgttca aaggtaacat ccccatcaac agtgattttc ccgtttgttg gtgcctgtag
     3001 caggttaatc acacgcacaa gagttgattt cccagcacct gaatacccaa caatgccata
     3061 aatatctcct tgattgatat gaaccgtgac atctttaaca gcttcgataa cccgtttttt
     3121 ttgacggaag gtaatatcaa tatgatctaa ttgaattatt gcttcattca taagatttta
     3181 ataactcctc tattagtacg atatggcgac agtaatctac gatagcaatg ttttcatcgc
     3241 ctgcataatc atgactatta agataacatg caatagtctt cccaaggttc ctaaatccta
     3301 aaagggaaaa ggtcttaaat agctttctaa aggtaagtta atcgtaaact cttcctcaag
     3361 catgcaagaa agcatgtgtt ttaaacacaa ccacactctt gttataacaa tggctaaaat
     3421 ttaccatacg gatacatcta taccatttga agttttttcc acgactttct tcacctcatc
     3481 agtttggtag gccttgatga gttttttaat agcatcagcc ttctctgatt tttcccaatc
     3541 tttttgacct gcaataatgt taatccattg ttttgaattg tcatctgctt tttctttgaa
     3601 aagagaggtt ttgtagtcaa tttttgcagg aacagcgtaa ctattattga caacagctgc
     3661 atcagcagat actaaagcac gtgccgtttg gctagcatct aattccttga tatctaattt
     3721 ctttttgttc tctgagatat tggcaatggt tgctaattga tcaccagaca cattcaattt
     3781 aatgaggcca gctgattgca gcacatagag ggcacggctt tcattggtcg catcatttgg
     3841 tactgcaatt tgagcaccat ttggcagatc agctactgat ttgtatttag ccttcccatc
     3901 ttgactggtt ccagagaaaa ggttaattgg gctgatatag gtatcagcaa tggccaccaa
     3961 atgttcctta ttttccttat tccagttatt taagaaatta tagtgttgga aggcattaat
     4021 atcgacttca ccattagcaa ctgccttatt aggttgcgag taatctgtaa attctttgta
     4081 cttcaaagtg atattgtctt tttttaataa ttcttcaact ttatcccatc tcgcttgatc
     4141 agattctgtt tttgtcatca ccccaatagt tagagtgttt ttatcatctt gtttttcaga
     4201 acaagcaacc aatacagttg atgccaaagc gattccgact attccaatcc attttttaaa
     4261 agacatagcg ttatcctcga ttcattttgt ttacgatcat tattgtatct taattagata
     4321 tagttttcaa attgataatg gctagcatat accatagttt taaactatat gctatgctaa
     4381 ccatccttat ttgtttagtc aacaaaaaac ttggtatttg aggactaaaa cccaaagcat
     4441 acttcttaca atattcactt ctcaaaaagc actttaacat ctcattttga ttaaggctat
     4501 ttgcaatttt tataacataa cctcataaac tataatattg tttccgatca gaaaaaccag
     4561 tcttctagat gaataactgg ttaatagatg gtttagttag ctgtaggaag tttcaactct
     4621 ttatctgatg gcacgtaatc accgccaagg tgctctttgg caagcttgct aagagttccg
     4681 tcagcagtca actctttaat acgcttgtta acaaagcttt gcaatttctc ttgatcttga
     4741 ctaaagataa aataaataaa tggttgctct gttgaggtga gttcaatggt tttgagatta
     4801 tctaaccctt gatttttaat aatggcgtta accgttggag cgtcaaaaat cttgaaatct
     4861 gccttgcctt cactcaaatt ggttaacatt tgagtaatgt tctcattggt gaattttaga
     4921 gtaaccgggt tgtcactgtg tttcttatta aagtcttcta actgtgcaac acttgtagtc
     4981 ccttgaacaa cttgtgtcgt atgcccttgg atatcatcaa agctcttgat atcactgtct
     5041 tttggaacaa ctaaaacaga aggtgttgat ccgattggat aagaaaaaag gtacttagct
     5101 gaacgctctt tggtaaagga aatgttgtta ccacccattt ggtattttcc tgagtccaaa
     5161 cctgtaaaga tagaagacca ttctgttttt ttaaaggaca ccttgtaatc atccgatcct
     5221 ttaaagactg ctttagccac ctcgatatcg tacccagtta gttgaccacc tttttcataa
     5281 gaaaagggag ctgttgttcc aaccgttgca aataagactt ctttagaact cccctgattt
     5341 cctgactcag ctgtttttga tgacccacaa gctgctagca caagggttga tgctagggca
     5401 attgttgctc ttcctaaata ttgtttttta atcatcctat ctctcctttt tgttcttgtg
     5461 tctagtttac catttttaat tagagttcac taataattga ttcctatcaa gttgatactg
     5521 atttactatt gttcattatc ttctaacaac gactatgtta cctggtactt acttataaaa
     5581 gcatcactta aaaagagctg aaatagattt caactctttt tattattcta tgtcaattta
     5641 aaaatctgct acattatgat acactttttg aacatcatca tctgattcga gtgcatcaat
     5701 aagcttttca aaagtttcaa ggtcatcacc ttccaatact acttctgatt gaggaatcat
     5761 ttcaagttca gtaacttgga attcttctac accattatcg cgaagtgctt ggataccttt
     5821 atgaagatct gttggggcag tgtaaactgt tattgttccc tcttctgctt caacatcatc
     5881 tacgtctaca tccgcttcaa gtaattgttc aaagacactg tcagcatcat caccagcaaa
     5941 aacgatgacc ccttttttat caaataagta ggatacagat cctgaagctc ccatattgcc
     6001 accgttctta ccgtaagccg tacgtacatt tgccgctgta cggttaacat ttgatgtcaa
     6061 agtatccaca ataatcattg aaccatttgg accaaaacct tcatagcgtc cctctacgaa
     6121 agtttcatct gtgtttcctt tagctttatc aatcgcttta tcaataacat gctttggcac
     6181 ttgtgcttgc tttgcacgat caatgacaaa tttaagagct gtatttaact ctggatctgg
     6241 ctcgccttgt ttggcagcca cataaatttc aacaccaaat ttggcataga cctttgatgt
     6301 tgctccatct ttagcagttt ttttagcaac aatatttgcc catttacgtc ccatttttgt
     6361 ctccttttaa tagtatttta ttgaatattg tcaacattaa cgttactaat attgactcta
     6421 actcccttat tatagcactt tgaaaagctt tggtaaaata gaaatataaa atcgttttaa
     6481 gcatcacact aaaaaccaaa taattaagtt cccatacatc aatattatgt tacagacaga
     6541 ttttggcgct tgagtgacaa cagcttccgg ttcacgacct cccgtttaat tagtattcct
     6601 ttaaaatctt gtcattcata acacacaacg ttctaaattt tggcaacatt ccattactac
     6661 aattgtcaaa atgattactg atataatctt gtaaaaacgt tagcctgttc ttcgaaagca
     6721 tatcttttga ctgacttttt taattattgt tataaaattt cctcattgga ttaactgaag
     6781 tataaaccat caacgtaatg ttagagtcaa gcccccaaaa aaaactcaat tagagtttaa
     6841 ttgagcctct cattttttat tataataggt attcttccaa aactttgttc tgggatatcc
     6901 tgatgcccaa gttgatagat gctgtctgct ttttgtgtca tctcatccca agtttctgct
     6961 ccaattctag taatcagagg tctgcttttt tttaattttt ttaaatgtgc ttgtcctttt
     7021 gaggtaaacc ctagaatatg aatcccctta ggtaaagtag gttccttagc attaaccaaa
     7081 atgtaggtta aaatacgtct tacacgagct tttgtatagc gtttggtagc aacagtgtca
     7141 actaggtggt caatattttg actaactttt atcgcttttt taatacgact cgccaattca
     7201 tcattgacct gaaaaatact agttaaatca gaatggttca aaatttgata ttttaagaat
     7261 gaaaaataat ggtcccatga catatgggga ttgtttataa gtaaacctgc atttggtact
     7321 gaatgactta tgaaatccca atcattcagg tgctgtctaa tggcacttgc agaagctagc
     7381 agatgatttt tgtctttcga atggtaggca gccccttgac gcttgatagg acatagttgt
     7441 atatgctttc cagcgctagc ctttgcgtaa cttaacccca aaatatgatt tggcgtttgc
     7501 cctgaaaatt taactccagc aaagatttcc cacatttttt gtgttttttg tggatagcta
     7561 agtgtatctt caagcgtcga taaataggca gtcatttgct ctgatttttt ctcatagacc
     7621 ttaatcagtt tttgataatc aatcacatcc tctgtcccaa atgctaattg ctgaattcct
     7681 aattgacaaa gaatatcaat agctccttga gcaaaatagt ctgccgattg aactgaaacg
     7741 aaaaagggca attccacaac aatatctgca ccatttttaa gagccatctc tgatcgaatc
     7801 catttatcaa taagagcagg ctctcctctt tgcataaaat ttcctgacat agcaatgatt
     7861 tttagtccct ctgctgtttc tagcaaatat ttgtgtccat tgtgaaacgg attaaactca
     7921 gctataattc cggtaacagt catagaatct cctcaaattg tactaaataa tcttttatag
     7981 ggctaataag agttaaatca aatgcttaag cacaacgtat aatagaaaat gctttcttta
     8041 tcgtaatgtc tccaatactt ctaagctttg ataatagcat ttaatgccta atgaactctt
     8101 ttcatataag taacattcaa gataattcat taaatcctat agctaaaaag gctacttttt
     8161 cttctattta tatgccacaa aaaaccaacg cttgctagtc tcagtcggct ccttatcctc
     8221 aaaatccgca tagaccttaa atgacttgaa ccctgcttgt tccaacaaaa tatcataagt
     8281 caataactca tacgttcgtt cctcatgcac ttcatcaaag cgagaaaaac gtccatcgtc
     8341 ttcttggata aagaaagtga gttcgtgaac aacagagtga ggaacctcgt cagcataggt
     8401 atcccaaacc attgcaaaat catctgcatt ctcatgatag gaataaccag ggaaacactc
     8461 atccgtttga taggttgaat gcacatcaaa aataaagatg ccatcgtttg ctaaaacatc
     8521 ataaacttct ttaaaaacat cgccaacatc aacttcgtct tgcatatagc aaatggaatc
     8581 cgaatagcag gttacaaaat caaactgccc cacctgtgac aaatccagca tgttaccttg
     8641 aataaaatca attttctttt tagcagattg cgcacgcttt ttagcaatag ccagcatgtc
     8701 ttgacttaag tctaaaccag tcacgtcaaa acctgcttga gcaaaacgaa ctgactgaat
     8761 accggttcca caggccaact ctaacaaacg atttcgactc ttagactttg gtaaatgtcg
     8821 aagtgaaaaa tctgtccaca agtcatatag agaatcatcc atcacagcat cataaactga
     8881 cgcaaatttt tcataattat gttccattat catcctcaaa aaccctgtcg tattgacaag
     8941 gtctctttgt aacattaagc taaataggca tctagagcca cagcaggcgc ttcatgccac
     9001 aatttttcaa gattatagtg gtaacgctcg tcttctaaaa aaagatgtac caccacatcg
     9061 gtcaaatcaa gcaagaccca gcctgcttgg ctattacctt caacatggct agcatctcca
     9121 ccagcctctt ttaccttttc acgaatatta tccgcaatag cctctaattg acgactgttt
     9181 gttgcactcg caatcacaaa ataatcagtc aaactagtta acccctctaa atccaaggct
     9241 aaaatatctt tagcacgctt ttcatcagtt gcttccacaa caatttttaa gagttcttct
     9301 tttttcattt tattcctcat atcattatcc attagtattc ctttaaatag ctgcaaaacg
     9361 cattataggt atccagagtt tgtgggaaaa taggctgcgc ttttgaggct aaataagcga
     9421 cagtgttcac tgtttcatag gcaacagcct gattcaaatc gagcttcgcg atttttctag
     9481 cgtcatcaac caaaggaaaa atgcgacctt cttcaatata atcagcaacg tataagacct
     9541 tatccagcaa ggtcatctca gcagcaccaa cggtgtgaat ttcaatagct cgcaagatat
     9601 ctttgtcttt tagccctaag tcttcttgaa tcttataaat accaaccata ccatgccaga
     9661 cattattatt ccatttagct aattctggag ataattggta cttgtcaatt aagtctaaaa
     9721 agacctggtc agggcactcc ttggcataat catgtaaaag agctgctaat cctgctttgt
     9781 ctgggttaca accataacac tctgccaagg acaaggctgc cttttccacg ccaagtacat
     9841 gtttaaaccg tttagggctc atctgctctg caatttttgc taacagttcg gtcctgctat
     9901 aaggaagata atcttcatat gtcattgata caaaccttct tgagtaatgt agtctaaaac
     9961 ccgcttaggt aagagataat tgggctgacg acctttttta ataaaatccc taatcatact
    10021 agaagaaata tccatcaaag gaagatctac ccaaatcact gggtaagagg ttccagcttt
    10081 atattttgga cgttgaaccc caacaaattg aacgagtttg accaattcat caatacggtg
    10141 ccactttggc aaataatcaa ccatatcagc tccgatgata aaataataat cgacatcagg
    10201 atgctgctca gtcaaataaa gcatcgtatc ataggtatag ctgattccct gacgttcaag
    10261 ttcacaggtt tcaatagcta gcccttcaac atcttcaata gccaattcca acatgcgaag
    10321 acgatgcttc tcatcaatag tctctttggc atccacatgc ggtggtttac attcaggcat
    10381 caatagcacc tgatctaatc ctaattgctg gcgaacttga tctgcaacca caaggtgtgc
    10441 attgtgaata ggattaaaat tccctcctaa aatcccaatc tgtttgcggt tgctttcttt
    10501 tttttcttcc tctaattcca ccttcgtaaa aggcgtcaac aattctaatg ccatactttc
    10561 tccaaaattc tcgcgacaat agtcctcgtc tcataaaacc agtccctata caaataaaag
    10621 gactagattg ctttaacttt aggggacaac ttgcggtttt ctttcttagc agaaacctta
    10681 tagagaatca agatacgacc aattttcaaa accgtatcac acccaatttc ttcttctaaa
    10741 atctctgcta cttcatgaat atcttcatca gtattttgca acaaagtgac ctttatcaat
    10801 tctctagcat ccaaagcttg acgaatacta gtcttaatgt ggtcatttaa tccatttttg
    10861 ccaatttgta caatcggttt taagctatga gcctcagatt ttaagaaagc tctttgttta
    10921 cttgtaagca tttttttcca acttcttttt tatttttaaa taatcgcttt acgaacaatg
    10981 acagcgaccc cttcaggagc ccatgcagca actatcgctt ttgagtcttt ttgaccatta
    11041 acacgaatcc atcctagacc agaaaaaaca atatccattt tctgatcaat tgtaaattca
    11101 tgacgaacta atttaggaaa tgcagtcaac tctttcttat ctggcggggt taacaaagtt
    11161 ccaacatgct tatcataaaa agcatctgct ccagctaatt ttgtacgatg taattctagt
    11221 tgattgtcaa aaaaagctgt aaatccttga cgctcaccgt tgataaagtc aaagcgagcc
    11281 agaccaccta aaaagagagt ttgttctgga tttaattgat acgtcttagg cttgatttcc
    11341 ttcttaggac tcactatttt tagctcttta ggggataaat aatgagccat ctggtgcctg
    11401 tgaataattc ctggcgtatc aaaaatgaac gtcccatcat ccaaaggaat ttcaattttg
    11461 tcaagagttg tcccaggaaa acgagacgtc gtgatgacat ccttgttacc agtaatctct
    11521 tggataatcg cattaatcaa ggttgattta ccaacattag tgacccccac aacataaaca
    11581 tctcgaccat tacgtaactc attgatacgc ccaatcaagt ctttaatcgc atatttattt
    11641 tgagcgctcg ttaacataac gtctaatggg cgaagacctt cttcgtgtgc acgttccgtc
    11701 aaccattgag taactttacc atccttaaca gactttggca aaatatcttt tttattccca
    11761 accagtaaga catcattacc agaaataaag cgagatagac ctggaataat agaaccatta
    11821 aagtcaaaaa tatcaatcac attaaccaca agagcatcac tatcacccac ttcgtgaaga
    11881 agtcttaaaa attcatcatc ggtaatatgg acatctgtaa tttcattata atgcctcaat
    11941 ctaaagcagc gctgacaata gagttcttct gtttccatac cctttttcaa agcagcagct
    12001 ggcgtaaaac cagctttttc tttatcttca gtttgaatct ggataccaca gcctatacaa
    12061 aataattctt ccattaaatt cctttttgat atgataattt accatatttc tcttctaatt
    12121 ttgccatcac acggcgttca cgccagcggt taattttagt attccaagca tcagaagcaa
    12181 caagtggttt gactaaaact gattttattc cagctctatg actagctctg atatcagtca
    12241 ttagctgatc tccaaccatg ataacctcgt tgcgatcaaa tccataacga gcaatggctt
    12301 tttcaatacc ataagcaaat ggcttcaaag cccggctaat aaagtcaaca ccaaagcgtg
    12361 aaacggcacg ctcaacacga gaatgtttat tatttgaaac aacaaccacg ctaatatccg
    12421 cgatagtcat ttcatcaagc caagcgcgaa cctctggtgt gccatcagga ttattccatg
    12481 caattaaggt attatctaaa tccactaaaa cagccgtaat accatgacgc aacaaatcat
    12541 ttgctcttaa atcataaata gcctctacca tataggtcgg cctataatca tcgatactca
    12601 tgtcatctcc aaaataatta gtactttcta ttttatcata ttttttttga aatagcattt
    12661 aaaaccatag ttaaatatac tagatatgtt tgacattaca actagatatg atacctcaac
    12721 tcgaaaagat aacaccttga tatgctcaaa aatagactct tctaatggtt agtgtaacac
    12781 aaaacactcg ttgtttgaca ctctataaat tcactgccaa gctatctaaa cgtaccacag
    12841 gactgatata caaaaatctt atcaagagtc aactcctgat aagataatca caaatgattt
    12901 cgttttgaac ttgtttagac gtatatcctt gacaaggata tcaaatacat ttaaatacag
    12961 tagatatccg caaaaagtgt taaacatatc agctcttctg acctatgccg atatttttct
    13021 atgactattc agtatttact atagtttata ctattttatc ccatacttgt gaacttactt
    13081 gtaaacagga cagtcgccag ataacacatg ataataactg taaatcaaaa aatgattac
//
LOCUS       DAUCWO010000047        12891 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUCWO010000047 DAUCWO010000000
VERSION     DAUCWO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.047"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            53..1351
                     /gene="ska"
                     /locus_tag="VGY20_001276"
     CDS             53..1351
                     /gene="ska"
                     /locus_tag="VGY20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ4274127.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGYEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSRPAHGGKTEQGLRPKSKMPYKLEKADLLKAIQ
                     EQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADKDDSVTLPTQPVQEFLLSGHVR
                     VKPYQPKAVHNSAERVNVNYEVSFVSETGDLDFTPLLRNQYHLTTLAVGDSLSSQELA
                     AIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQAYKANSKT
                     GIEEKTNNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTINYVDVNTNKLLKSEQLLTA
                     SERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIITVYMGKRHE
                     GENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPKDK"
     gene            complement(1449..1892)
                     /locus_tag="VGY20_001277"
     CDS             complement(1449..1892)
                     /locus_tag="VGY20_001277"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ4274128.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     RQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(1907..4126)
                     /locus_tag="VGY20_001278"
     CDS             complement(1907..4126)
                     /locus_tag="VGY20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ4274129.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(4378..5712)
                     /locus_tag="VGY20_001279"
     CDS             complement(4378..5712)
                     /locus_tag="VGY20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4274130.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEISTATM
                     TQQERAEKIKKIKDYITKYPKTTEERFWNRTWIRDYFKDEDYLKAVQSFTEQALENIL
                     ELIEKGATKGEKGDAGPAGPRGERGPQGKAGEKGETGPRGERGEQGIQGEQGKAGEKG
                     EKGDKGDTGERGEKGETGATGAQGPQGEAGKDGAPGEKGEKGDRGETGAQGPVGPQGE
                     KGETGAQGPQGEAGQPGAQGPAGPQGEAGKPGEQGPAGPQGEAGQPGEKAPEKIPEGE
                     AGQPGEKAPEKSPEVTPTPETPEQPGEKAPEKSPEVTPTPETPEQPGEKAPEKSPEVT
                     PTPETPEQPGEKAPEKSPEVTPTPEMPEQPGEKAPEKSKEVTPAPEKPADKEANQTPE
                     RRNGNMAKTPVANNHRRLPATGEQANPFFTAAAVAVMTTAGVLAVTKRKENN"
     gene            6094..6576
                     /gene="nrdI"
                     /locus_tag="VGY20_001280"
     CDS             6094..6576
                     /gene="nrdI"
                     /locus_tag="VGY20_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ4274131.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFSLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(6970..7788)
                     /locus_tag="VGY20_001281"
     CDS             complement(6970..7788)
                     /locus_tag="VGY20_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ4274132.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMDDETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVAAHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(7871..10057)
                     /locus_tag="VGY20_001282"
     CDS             complement(7871..10057)
                     /locus_tag="VGY20_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ4274133.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSVLFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDRNTQARPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTNGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLMHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTAEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(10414..11163)
                     /locus_tag="VGY20_001283"
     CDS             complement(10414..11163)
                     /locus_tag="VGY20_001283"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ4274134.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTSSMAHELEIIEALPDQVELPVEVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYSADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKEEFLKNLPS
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(11163..12116)
                     /locus_tag="VGY20_001284"
     CDS             complement(11163..12116)
                     /locus_tag="VGY20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ4274135.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYTGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELVSFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQDNIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMIQGEWNALVFKKTDDISGVIGG"
     gene            complement(12133..>12891)
                     /locus_tag="VGY20_001285"
     CDS             complement(12133..>12891)
                     /locus_tag="VGY20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274136.1"
                     /translation="RELLMFNTALLFISNTYTFSLPIELLLVFLLIILSIFSAVIDTK
                     KELQQPGRLFSFLLSIVGLIMLLGALKQFLDNLSDIKSFDFWLSYAFELLVILINLPV
                     LYIAQKMIIIEKIIVHSEYPNTIVSFMRYYYKWYCRKIKFKKLIVKDYNLDIAVQKYI
                     FGYPKISVYVKEGNLSKEKVLNLIALIIVKGDKKEKLSRRIDRFPVYIEVVDKENQTV
                     ALWTEEFLSKQNYFYDPFMTKNTKEIYPSILMLQ"
BASE COUNT         3469 a         3001 c         2513 g         3908 t
ORIGIN      
        1 tattatgtta ttctaataat ggggattgaa acttaacttt taggaggttt ctatgaaaaa
       61 ttacttatct tttgggatgt ttgcactgct gtttgcatta acatttggaa cagtcaagcc
      121 tgtccaagct attgctgggt atgaatggct gctagaccgt ccatctgtca acaacagcca
      181 attagttgtt agcgttgctg gtactgttga ggggacgaat caagaaatta gccttaaatt
      241 ttttgaaatt gacctaacat cacgacctgc tcatggagga aagacagagc aaggcttacg
      301 tccaaaatca aaaatgccat ataaactgga aaaagctgac ttattaaagg ctattcaaga
      361 acaattgatc gctaacgttc acagtaacga cggctacttt gaggtcattg attttgcaag
      421 cgatgcaacc attactgatc gaaacggcaa ggtctacttt gctgacaaag atgattcggt
      481 aaccttgccg acccaacctg tccaagaatt tttgctaagt gggcatgtgc gcgttaaacc
      541 gtatcaacct aaagccgttc acaactctgc tgaacgcgtt aacgtcaact atgaagtgag
      601 ctttgtctcc gaaacaggag atttagactt tacaccgttg ttaagaaacc aataccattt
      661 gaccacactg gcagttggtg actctctttc atcacaagag ttagcagcaa ttgcccaatt
      721 tatcctatca aaaaaacatc cagattatat cattacaaaa cgtgactcct caatcgtcac
      781 tcatgacaat gacattttcc gtacgatttt accaatggat caagagttta cttaccatat
      841 caaagaccgg gaacaagctt ataaggccaa ttctaaaaca ggtattgaag aaaaaacgaa
      901 caacactgac cttatttctg agaaatatta cgtccttaaa aaaggggaaa agccgtatga
      961 tccctttgat cgcagtcact tgaaactgtt caccatcaat tacgttgatg tcaacaccaa
     1021 caaattgcta aaaagcgagc agctcttaac agctagcgaa cgtaacttag acttcagaga
     1081 tttatacgat cctcgtgata aggctaaact actctacaac aatctcgatg cttttggtat
     1141 tatggactat accttaactg gaaaagtcga ggataatcac gatgacacca accgtatcat
     1201 aaccgtttat atgggcaagc gacacgaagg agagaatgct agctatcatt tagcttatga
     1261 taaagatcgt tataccgaag aagaacgaga agtttacagc tacctgcgtt atacagggac
     1321 acctatacct gataacccta aagacaaata accacgatct tctaaaacga tgagattaac
     1381 tgacaaaaaa agcaagcaac acgctatcaa cagttgcttg cttttttcta atctcttagt
     1441 tgtagagact agtgacattt cgtgtctaaa ataatcgtaa ctggtccatc attgatgaga
     1501 ctaacctgca tatctgcccc aaaaacgcca cgctcaactg gcacaaactc tgccaattgt
     1561 tcattaaagc tatcatacaa ctgcctagcc aaatctggct tagcagcacc agtaaaggca
     1621 ggacggtttc cctttttggt gtcagcataa agggtaaatt gcgacacaga taagatactg
     1681 cccttgatgt cttggataga ctgattcatc ttgccatcag catctgaaaa aatgcgcatg
     1741 ttgactattt ttcgcacagc gtaagccaaa tcttctgcat tatcatctgg tcccacacct
     1801 actaacaaca acaatccttg attaatagcg ccagcaattt tcccatcaat agaaacagag
     1861 gcctctttga cccgttgcaa gacaagtttc atgtttttat cctttcttag ccattggtcc
     1921 gcttcacact ataaacatct ggaactgcct tgattttttc gacaacagtg gtcagatgcg
     1981 tcagatttgg aatgccaaag ctcacgtgaa tattagcaaa cttcatgtct ttggtcggct
     2041 gagcattgac tgtcgatatg ctcttggttg agtttgataa aatttggagc acatcattga
     2101 gcaggccact acgattgagc ccatagatgt caatttcagc ctgataatct ttactcgaat
     2161 tgtccaaatc ccactcgacc tcaatcaagc gttcttggta gccatcttga ctcttaatgt
     2221 tatgacagtc cgatctgtga atcgcaatgc cacgcccttt ggtaatgtag ccgtcaatag
     2281 gatcaccagg tacaggatta caacacttgg caatccgcat caagaggcct gatgctcctt
     2341 ggataatgac tccattttca ctgcgaacct tgagcacatc tttgttttcg tgtttgacct
     2401 caccgccctt aaccaattct tcagcttctg ctttagcctt ggccctttct tcttcacggc
     2461 gctctttttc ggttaacttg ttaaagacac tgataggact aatgtcacca aacccaacgg
     2521 ctgcatagag cgattcttcg ctcttcacac tgacttttgg aaggatcgct tcaatgcgtt
     2581 ttttgtcaag gtatttattg gcaacgtagc cctgctcttg aaaataagac accaacaaat
     2641 cacggccttt attcactgac aattccttgt cttgattttt aaagaactga cgaattttgt
     2701 tgcgagcctt attggttttg accagtttta cccagtctcg actagggcca aaggaattgg
     2761 cattggtgat gatttcaacc acatcgcctg tttttaactt ggcagtgaga ggaaccatac
     2821 gtccattgac tttggcacct gttgcttttt caccgatttg cgtatggatc gcataagcaa
     2881 aatcaatagg acctgattct tttggcaact cctgaacggc ccctgtcggt gtaaagacat
     2941 aaatccgttc agaaaaaatg tcttctttga ccgaatccac aaagtccact gcatcaccat
     3001 ttgaggcatc ttgcaattct accagctctt tgatccagtt catgccaacg gcttgctcag
     3061 cctgattgac cttaccacgc acgccttttt tataagccca gtgagcagca accccgtact
     3121 cagccacttg atgcatgtcc ttagttctga tttgaatctc aataggtcct tttggtccat
     3181 acacggtggt atgaatcgac tggtagccat tagctttagg agctgcaata taatccttga
     3241 agcggcctgg catgggacgc caaagctcat gaatatagcc aaccatagca taaacatcgc
     3301 tctgcgtttc catgacacaa cggatagcaa tcaaatcaaa aatctgatcg aaacgtttct
     3361 ttttgtcccg cattttccga taaatggaat aaatgtgttt tggtcgacca tacacatctc
     3421 caaacaagcc ttgttgtgtc gtataggttt tgaccttact gacaatagcc tctaccaaag
     3481 cttcacgctc gcgacgtttt tctttcatca tatgggaaat tttgtaaaat tcggtttcat
     3541 tgaggtaacg aaaagacaaa tcttctagtt cccatttgat gcgactaatc cccaaacgat
     3601 gcgccaaggg ggcatagatt tccatggttt cgcgcgaaat acgctcttgt ttgtccttgc
     3661 gcaaatgttt gagggtgcgc atattatgca ggcggtcagc caatttcacc aaaatcacgc
     3721 gaatatcttt ggacatagcc atcagcattt tgcgatggtt ttcggcgagt tgctcctcat
     3781 gagatttgta ctcaacttta cctaacttgg tgacaccatc aacgatatca cgagcatctt
     3841 ggccaaagtc tgcttcgatc tcatctaagg taatatccgt atcttctacg acatcatgta
     3901 aaaagccaca ggcaactgtc acagcatcca gatgcaaatc agccagaatc cccgccacct
     3961 gaatcggatg gacgatataa ggttcgcctg actttctcac ttggtagaaa tgggccgctg
     4021 ttgcatatgc taaagccttt gccacaaaag ccacatcagc cttggtcata taggtggccg
     4081 ctaaggcaat gacttcttct cctgttacgt tcattatttt tgccatttcg ttcctcatct
     4141 agcatttctt ttccctattt taacacttta cacacaaaag caaaaggcag aatctgctaa
     4201 gaaagcgctt agtgtgacct tttcttagaa aacggttagt gagctgcttt tgatatcttt
     4261 tgggatctct caggcgtgag catgatacca aaggcctgct cccgaccaaa aatagagaga
     4321 accaaggggc tctctctatt atgtttgctt atcagtaagg cctctcaatt tagaggatta
     4381 gttgttttct ttacgttttg taacggctag gacaccagct gttgtcatca ctgctactgc
     4441 tgctgctgta aagaatgggt tggcttgctc accagttgct ggtagacgtc tgtggttgtt
     4501 ggctacaggt gtcttagcca tattgccatt gcgacgttct ggcgtttggt tagcttcttt
     4561 gtcagcaggt ttttctggag ctggagttac ctctttgctc ttttctggag ctttttcgcc
     4621 tggttgctct ggcatttctg gcgttggtgt cacttctggg ctcttttctg gagctttttc
     4681 gcctggttgc tctggcgttt ctggtgttgg tgtcacttct gggctctttt ctggagcttt
     4741 ttcacctggt tgctctggcg tttctggtgt tggtgtcact tctgggctct tttctggagc
     4801 tttttcacct ggttgctctg gcgtttctgg cgttggtgtc acttctgggc tcttttctgg
     4861 agctttttcg cctggttggc ctgcttcgcc ttctgggatc ttttctggag ctttttcgcc
     4921 tggttggcct gcttcacctt gtgggcctgc tgggccttgc tcacctggtt tgcctgcctc
     4981 accttgtggg cctgctgggc cttgtgcgcc tggttggcct gcttcgcctt gtgggccttg
     5041 tgcgcccgtt tcaccttttt caccttgtgg gcctactgga ccttgagctc cggtttcacc
     5101 gcggtcacct ttttcaccct tttcgcctgg agctccatct ttaccagctt caccttgtgg
     5161 gccttgtgcg ccagttgctc cggtttcacc tttttcaccg cgctcaccgg tatcaccttt
     5221 gtcgcctttt tcacccttct cgccagcttt accttgctcg ccttggattc cttgttcacc
     5281 gcgctcacct ctcggaccgg tttcaccttt ttcaccagct ttaccttgtg ggccgcgttc
     5341 gcctcgaggt cctgctgggc cagcatcccc tttttcacct tttgttgcac ctttttcaat
     5401 cagttctaaa atattttcaa gtgcttgttc agtaaaactt tgtactgctt ttaaataatc
     5461 ttcatcctta aaatagtcac ggatccaggt ccgattccaa aatctttctt cagtggtttt
     5521 tggatatttg gtaatatagt ctttaatttt tttaattttt tcagctctct cctgctgtgt
     5581 catagtcgca gtagaaatct cagccttaac gctgcttgcc ccgcctccaa aggctagaag
     5641 gacggcagcg gctgaggtta gcccgtagcg ccagaccagt ttgttgagat tatggtgctt
     5701 tgatgtcaac atatgttgtt ctctctttct ttattagatg gtgtgtttta gataaaaata
     5761 acatggtagc tagacctgat tatttataat agtattataa tagagtgtga aaacgattgt
     5821 aaagttctct tttgttgttc atcttttaga ttttgactga tcgctctttt gtggagatca
     5881 gagggccact ttagcacttg cctgcctcgt ttttaagttg tgggcatccg gatcctttct
     5941 ttaaggtcac tcttttttga aatgttactt tttttgactg cttgtacctt cttgtctggt
     6001 tggataccgc taggtttctt ctttacttgt ttgttggtct tgattgtggt aagcttatga
     6061 agaaaccttg taggataaaa aaaggagaca cttatgcctc agattaccct tgtttttatt
     6121 agcctcagtg gcaacaccct tagctttgtc aagcggctat ccctttatct agcagacaat
     6181 tacgattacc atgtcaaaca gattaacatt aaagatttga agcacgagac ttttcccgtc
     6241 aaagaagagt ttgtggcgat tctacccact tatctagaag gcggaaatgg cgttgactct
     6301 ggtgaggtgg agatcctcac cactcctttg ggagagttta ttgctgccca tggcaacgcc
     6361 cagcgttgtc ttgggattat tggttcaggc aacaaaaact ttaaccatca gtattgcctg
     6421 acagccaaac aatatgccaa acgctttggc ttttcactgc taggtgattt tgagctgcga
     6481 gggactcctg atgacatttc ccggttagcc caggtcatca tggaggctag ctccagacac
     6541 tcatctaacg acacacagac actgcccaac agctagacag tctatagatg atcatagctg
     6601 ttttaaaaaa gagcgctttt aggacaataa ctacagaggt ctcttacccc acgccttgtg
     6661 atttctcagc ctttgaaaag agcgatcaca gtgcttcttg gtgcttggga tcacaccaag
     6721 cactcctaga aataccccca tgcagacttt agcaaggcaa ctgaacgctt ggcggtactt
     6781 tctgtgaagc tcagatggtg ggtgacaaaa aagccctggt tttttagggt tactgacatg
     6841 actaaagagg tctaaccagc tctaccttga gctagttaga cctctttgtg ttctcctaat
     6901 ccccctagat gatcgcagtt tcttatgtgt cttggcttct cctatgtggt gtgcctgtta
     6961 gcacatgctc taacgccagt ctaatgtcac ttctagaccg tagtggtcac tgacgactgg
     7021 cgcatctccg ccttcaaagg taatcttaga agatcggatg atgaagtcct tactggtaaa
     7081 gacatgatct accttgaggg cttctttgtt accttgccag ccatcaatat cagccacgat
     7141 gctatgatca ccaaacacgt gtgcagctac tttgtgacta tcttgtaggt ctagggggct
     7201 acccatcatc acttgataac cgtcttgatc cgttggattg ttaaaatctc ccataagtag
     7261 caagggacag tttagtgtga gcagctcttt ttctaatttc tcccactccc ctaaaaaccc
     7321 tttgtcaaac caagataggt gcacattgac aacagcgact tctttgccat ctaacgtggt
     7381 tttggctatg agtgagcgtc tggtgtggta gtctgtttca tcatccatcg ctgacacgag
     7441 aatatctgaa acatgaatag gttgttttga taagattgcc acgccttcct ggtaaatgtc
     7501 ataaccaata tggttataag cccaagacca ataatagtgc tgccctcgtt tttgtaaata
     7561 atggatcaag agcagagcaa aatggtcttt atgaatgctt ggtgtccctg aaagagcttg
     7621 atatcttggt aaatctgtgg ccagctcgct ctcgattaac tgattgattt cttgcaagca
     7681 aatgatatcg tatttttcag ctaaaatatg ctcagccaaa gcaactagtt ttttaagagt
     7741 gttggcttgc atccacgaat gggtattcag tgttaatacc ttggtcatta atcctccttt
     7801 ataagacaag aagagccata gggcctaaac cagctatggc tattacctat cactatctcg
     7861 tgatgttttg ttaaagttct acggtggcta ctttagtctt agcaggttga ttgcctaaag
     7921 aagtcaaggt cacatcttgg atctctgctg tgttggtaaa agcaacaaca atggttgttt
     7981 cacgctctgc tgatttaata gcagcaagat ctgctacgac taataggtca cctgcatgaa
     8041 cccgttgccc ttctgagact ttgactgaaa aaggcacacc atttaaggcg acggtatcaa
     8101 gtcccacgtg catcagcact tcaagaccat tatctgtcag taaaccaaag gcatgcttgg
     8161 tcggaaagac actggtcaca aggccagcta cgggtgcata gatattacca tttgttggtt
     8221 caaccgcaaa cccatctccc atcatcttag ccgcaaacac ctggtctttc acacctgtga
     8281 ttgggagaac catcccgtct gccactgata gcacatcttc tgtcacgtgt ttaaaatctt
     8341 ttgcgggagt gggtttgcta gtgagctgtg acatattgac ttctggaata acagcccctg
     8401 agtccagcaa atcttgaata tctgatttca aaatatctgc tttagggcca tagaccgctt
     8461 gaacgccgtt gcctttttgg atcaagccca tagcgccagc ttttttccaa tcgtcctcag
     8521 cgccaacttt agcggggtct ttgaccgtca ctcgtaagcg cgtcatacaa gcatcaacat
     8581 catcaatatt gcctgcccca ccaaggagat taatgatttg tacgacttga gagttgctat
     8641 ctgctacttg ggtaggtctt gcttgagtgt tacggtcacc cagtatatcg gcatcgtagt
     8701 tgcctaaacg gccagctgtt gcgagatgca ttttcttgat catcatatct gcaataaagt
     8761 acatgataac tgcaaagagg acagaaaccc aaacaaagtt aatcacatcc atgcctaggc
     8821 ctgctttaag agccataggg gtacgggtta gtagctcaat gttcccaaat gagtgaacac
     8881 gaaggttcac aaggtcagcc atagcaaatg aagctccttg aacaagagca tagacgatgt
     8941 aaagtggcat tgctgcaaac ataaataggt actcaagtgg ctcagttacc cctgtcaaaa
     9001 agaccgctgc cgctgctgag atgaacatca ttttgtatgt gtgtttttta tcggcatcta
     9061 cattgcggta catggctaag gcaaccccca tcaatgttcc ggtagctcca atcatttgtc
     9121 ctaccttaaa gcgagctgga gtcacactat ccattaagtg actataggct gaagcgtctg
     9181 atcctttaag gtgaacaagg tcagttaccc aagcaagcca caaagggtct tgaccaaata
     9241 cttttgtccc cgctgctgca cctgtcatga cctcataagt tccaccaaga gctgtatagt
     9301 tcattgggat cgtcaacatg tggtgaagac caaatggcaa caagagacgt tccaaggtac
     9361 catataggaa tggggctagg attggagctg aatcttgtga agaggcaatc cacatcccaa
     9421 aactgttaat cccagactgg atcaccggcc aaacaacaac caaaataagg gctacaaaaa
     9481 tagaacgtaa aataacgaca aacggtacaa aacgcttccc attaaagaag gtcaaaactt
     9541 caggaagttt acggtaatta tagtatttgt tataggccgt tgctcctacg aaccctgcaa
     9601 taatcccaac aaaaacccct gtgtttaaag ctggagattc caagacactg gtgaaataat
     9661 ctttaacgat catttgagtg ccaaggaggc ttgtgatttt tgcttctgga tcagctagca
     9721 tagcactaga cacgccataa aaagcacctg ttatccgatt gatcaagaca aaagcaagac
     9781 ctgaggcaaa ggcccctcca gctctttctt tagcccaact accaccaatg gccaaggcaa
     9841 atagcaaatg aaggtttaca ataacagccc agccaatctg agcgataatg ttgcctagag
     9901 atgccaaaaa tgctgagtcg tggttaatca ttggaataga gtttccgata ctgatcatta
     9961 aaccagccgc tggcatgaca gcaataacga ccataagaca tttcccaaat ttttgccaaa
    10021 attcgaaacg aaacagttgt ttgaatgatg ttttcattct atctctcctt tattatcccc
    10081 ttttagccat agctgccctt aaggcacttt tgtgacacaa tgatatagac tacaactcag
    10141 taatgcaatc gtttgcattg atctgatacc tttattatac tagcctcgca acaatttgta
    10201 aagcgttttc ttagacagat gttagtcgtt tttcttcatt aaatggtagt gtaactcacc
    10261 aaaaatatga caagagccca tttccttttt tggtgatgag cccatcaacg ctcttcaaaa
    10321 aaacaagaag caataaaaaa aggccgttga cacaatcgtg aactgcctgt tcgtttcttt
    10381 agcctcttct ttctaaagta agcagcagct tacttatttt gatgcctgca attccaaagc
    10441 ataactaaga gcacttaagg catatagcgg cgctgtctct gctcgcataa tgcgagggcc
    10501 taagcccacc tttatggcac ctgctgcctc aaactgagtg atttcagttg gggaaattcc
    10561 cccttctggc ccaaagataa agaggatttt tgcccctggc ttaacttctt ttacttcacg
    10621 cgccaaggtc gctaactgac ctgccttggc tgtttcttcg tatgcaacga aaatatgatc
    10681 aaagctagga aggtttttta gaaactcttc tttgtgttca aagaggtgaa cttcaggcac
    10741 cctatttcgt ttgctctgct cagccgctcc taatacaatc ttagccaatt tgtcttcttt
    10801 tttagctaat tttttgccat cccatttgac cactgaccag tctgcggagt agccccatag
    10861 cgcactggct cccaattcag tcactttctg ggcaatcgtg tctaacttat cgcctttagg
    10921 aaatccagaa gcaatggtca cctcaacagg caattccacc tgatctggca aagcctctat
    10981 aatctctaac tcatgagcca tactactggt cactttggct aaatacttca ccccatcatc
    11041 aaataccaag acaacttctg cctcgtcagt caagcgcatc acttgaaaca tgtgcttgat
    11101 ggtatcttta tcggtaatcg ttaccttttt ttctgctttg cctttaataa aatactgttg
    11161 cattagccgc caatcactcc tgagatatca tctgtttttt taaagacaag ggcgttccac
    11221 tcgccttgaa tcatgtgtgt ctcaaggaag aagcctgcgc taaaggcagc ttccagaacc
    11281 atatccaatt tctcagaaat gatgcctgat aagataaggt aaccttcctt tttgaccaaa
    11341 cggtaagcat catcagtcaa taagactaag atatctgcca aaatattagc cacaatcaca
    11401 tccgcttctt ggctaacccc ttttaaaagg tcaccagcag ccacatgaat gttgtctgtt
    11461 ccttggttta aatcaatgtt gtcttgagcc actcgaacag ccacgtcatc taagtcataa
    11521 gcgtagatgg ttttagcacc tagtaaggaa ctggcaatcg agaggacacc tgatccggtc
    11581 cctacatcga taactgtctc accaccacga aggacttgtt ccaaggcaaa aaggctcatc
    11641 ttagttgtgg gatgtgtccc tgtcccaaat gccattccag gatctaactt aataaccttt
    11701 tccccagcag aagcgtcata atctgtccaa ctagggacaa tggttaaatc atgcgtgata
    11761 cgagctggtt catagtattt tttccaattg tctgcccagt cttcctcagc caattcctga
    11821 ctgtccacag taacctgacc aacctgtaac ccaaaactaa ctaactccgc taactgttca
    11881 ttaatggtcg caataacatc cgccaaatta gtgctacttg ggtaataggc tgtgatagct
    11941 atcatatcag actgctccac atctgggtat aactccccaa agcggtcctt ttgcccagta
    12001 tagtctgcgc tatccgcgat agcgacccct tgactgcctg tctcaatcaa cacatgtgaa
    12061 acagcctctt gcgcatcacg atgcacatga accgtcactt cttgccacgt ttccataagt
    12121 atgtcctttc tcctactgca acattaaaat cgatggataa atttcctttg tatttttagt
    12181 cataaatgga tcataaaaat aattttgttt agataagaac tcctcagtcc ataatgctac
    12241 tgtttgattc tctttatcaa ccacttcaat ataaacagga aatctatcta tccttcgaga
    12301 taatttttcc ttcttatctc cttttacaat tattaacgct attaaattca gaaccttctc
    12361 tttagagagg ttaccctctt taacgtatac agaaatttta gggtatccaa aaatatattt
    12421 ttgtactgca atatctaaat tataatcttt tactattaat tttttaaatt ttatttttct
    12481 acaataccat ttataatagt atctcataaa actaacaata gtatttggat actcactatg
    12541 aacaattatt ttttcaataa ttatcatttt ctgtgctata tacaatactg gaagattaat
    12601 cagtatgaca agtaattcaa aagcatatga taaccaaaaa tcaaatgatt taatatcgga
    12661 aagattgtcc aaaaattgtt ttaacgcacc aagtagcatt atcagtccta ctattgataa
    12721 taaaaaagaa aataatcgac caggttgttg gagttctttt tttgtatcaa taacagctga
    12781 aaatattgac aaaattatca ataaaaatac taacaataac tcaataggta gagaaaatgt
    12841 ataggtatta ctaataaaca ataacgccgt attgaacatt aataattctc t
//
LOCUS       DAUCWO010000048        12702 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUCWO010000048 DAUCWO010000000
VERSION     DAUCWO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.048"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            8..706
                     /locus_tag="VGY20_001286"
     CDS             8..706
                     /locus_tag="VGY20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274137.1"
                     /translation="MFSGHQLKTARLSRGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(841..2403)
                     /gene="ffh"
                     /locus_tag="VGY20_001287"
     CDS             complement(841..2403)
                     /gene="ffh"
                     /locus_tag="VGY20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ4274138.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2418..2759)
                     /locus_tag="VGY20_001288"
     CDS             complement(2418..2759)
                     /locus_tag="VGY20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ4274139.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(2848..3546)
                     /locus_tag="VGY20_001289"
     CDS             complement(2848..3546)
                     /locus_tag="VGY20_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4274140.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3629..5062)
                     /locus_tag="VGY20_001290"
     CDS             complement(3629..5062)
                     /locus_tag="VGY20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274141.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKKPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            5229..6791
                     /gene="guaA"
                     /locus_tag="VGY20_001291"
     CDS             5229..6791
                     /gene="guaA"
                     /locus_tag="VGY20_001291"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ4274142.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELHEINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEITK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(6833..8056)
                     /locus_tag="VGY20_001292"
     CDS             complement(6833..8056)
                     /locus_tag="VGY20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ4274143.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDRAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(8421..9964)
                     /locus_tag="VGY20_001293"
                     /pseudo
     CDS             complement(8421..9964)
                     /locus_tag="VGY20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(10098..10655)
                     /locus_tag="VGY20_001294"
     CDS             complement(10098..10655)
                     /locus_tag="VGY20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4274144.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            complement(10633..11499)
                     /locus_tag="VGY20_001295"
     CDS             complement(10633..11499)
                     /locus_tag="VGY20_001295"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ4274145.1"
                     /translation="MKRIVVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEDSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            11589..>12702
                     /locus_tag="VGY20_001296"
     CDS             11589..>12702
                     /locus_tag="VGY20_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ4274146.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVID"
BASE COUNT         3936 a         2437 c         2140 g         4189 t
ORIGIN      
        1 ctaccaaatg ttttctggac atcaactaaa aacagcacga ttatcaaggg gaataactca
       61 atcagaattg ggaagattgt tgcatgtcaa taaaatgaca atatctaatt gggaaaaagg
      121 taagaatata ccaaatgaaa aacatttaaa tgccttattg catctattca atgtgacatc
      181 tgattatttc gacccaaact atagattgct aacgccttat aaccagctga caatatctaa
      241 taaagaaaaa gtaattggct attcagagcg attgttaaat catcaaatag acaaaaaatc
      301 taaagatctc atagataaac catcacaatt atatgcttat cgggtctatg aaagtttatc
      361 tgctggtact ggttactcct attttggtga tggtaacttt gatgttgtct tttacgatga
      421 acaattagaa tacgattttg cgtcttgggt ttttggagat tctatggagc caacttattt
      481 aaatggcgaa gttgttctta taaaacaaaa tagttttgat tacgatggag caatttatgc
      541 agtcgaatgg gatgggcaaa catatatcaa aaaggtattt cgtgaagatg agggattacg
      601 tctagtgtcc ttaaataaaa aatattctga taagtttgct ccctatagcg aagaacctcg
      661 cattattggc aaaattatcg ctaattttag gcccttagaa atttaagtta gattgattag
      721 gttatttcaa attttaaatg aactcatcat gcatgtaacg cggtcttcca taattttaat
      781 aatcttgtat acaagataaa tgaatttcac atcaaaaaga gacgaaaatt tccgtctctt
      841 ttattttcgt tttttctttg ctttttttag tttgttagct tgacgtttca tggcctgttt
      901 catggcaaat tgtccaattt tccccttgaa ccctttacca aatagttgac tcatatccat
      961 atcaccacca aggccagata aatcaggcat accaccttgt cccatcatcc cttctaagga
     1021 agacatatct ggcataccag caggcatgtt tttaggaaga ttattgggat taatgcccat
     1081 atctttcatc atcttagaca tatcgcccga cataacccct tgcatcatgc ttttggcttg
     1141 gttaaagtct ttgataaatt tattaacttc cacaaagctg ttcccggaac cagctgcaat
     1201 acgacggcga cgactcgggt ttagtaagtc agggttttca cgttcagcgg gggtcatcga
     1261 agagacaata gcacgtttgc gagcaatttg gttttcgtca actttgatat tggccaaagc
     1321 tgggttacca gccattcctg gaatcatttt caagaggtct tccataggcc ccatattttg
     1381 cacttgatct aactgttcaa tgaaatcatt gaaatcaaag gtgttctcac gcattttttc
     1441 agctaactct aaggattttt tctcatcata ttcctggcta gctttttcaa taagagtgag
     1501 taagtcaccc attcccaaaa tccgactgga catgcggtct gggtgaaatg tttcaatatc
     1561 tgtgattttt tcaccaatac ctgtaaattt aattggtttt ccagtgattt cacggacaga
     1621 tagtgctgca cctccacggg tatcaccatc aatctttgtt aacacaaccc ctgtgatgct
     1681 caactgatga ttaaactcgt aggctacatt ggcagcttct tgaccaatca tgctatctac
     1741 aaccaaaaga atttcatttg gttgtgctaa agccttgaca tcgcgcagct cttccatcag
     1801 tttttcatca atttgcaagc gacccgcagt atcaatcaag acatagtcat tgcggttttc
     1861 acgagcttgt tccaaacctt ttctgacaat atcgactgct gagtggtctg tccccatatc
     1921 aaaaacaggt acattgattt gttgccccag tgtttttaac tgatcaatgg ccgctggacg
     1981 gtaaatatcg gcagcaatca tcagtggtcg cgcgttttct tctttaatta atttattagc
     2041 gagttttcct gcaaaagtgg ttttaccagc cccttgcaaa ccgaccatca tgataatagt
     2101 tggaattttt ggagatttat caatctccgc ggtttctgag cctaaaatac tcgttaattc
     2161 ttcattaaca atcttaagaa tttgttgtgt tggatcaaga gtatcaataa tctcatggcc
     2221 aatagcgcgc tcacgtacgc gcttaataaa ggttttaaca actggtaaag cgacatcggc
     2281 ttctaaaaga gctagacgaa tctcttttgt cacttcttga acatctgatt ctgataattt
     2341 ctttttaccg cgaatgtgtt taaacacatc ttgcaaacgt tgggttaagc tttcaaaagc
     2401 catagtcaaa actcctctta ttctctattg tcaatactgg ttagaatgga aattttttct
     2461 tgcaaatact catcatgagg gtagtgagca atcatgtcgt caaagatttc acttcgaaca
     2521 acataatccg aatacatatg aagtttcatc tcataagtct ctaaaatttt ttccgtacgc
     2581 ttaatattat catagacagc ctgacgactg acaccaaatt catcagcaat ctcagctaag
     2641 ctataatcat cggcataata cagctcaata taattcattt gtttatctgt tagcaaagct
     2701 gcataaaatt caaaaagggc attcatcctg tttgtttttt caatttccat aatgttcatt
     2761 ttatcatact atgattgttt ttaacagtag caaattaatt gccttatgct gttcgtgtaa
     2821 aaaaggaagt cataaatgac ttctttacta attattttct aagtaaaatt caaaacggtc
     2881 accgatatac tgactgcgga catactcaaa aggtttgccg tctgtaaagt aagacacctg
     2941 agttagagcc aaaatggcat gtcctttagc aacttccaag taggaggcta cacgctcact
     3001 tgctagctta gcgtagattg tctgtttact tttaccaatc tcgtaaccat ttgctatcaa
     3061 tgagtgaaaa aagtgttctg taatatcagc tcgtttaacc gtttttataa atttttctgg
     3121 gattgaagca atttcgtaca ccaaagggac actgtctgca tagcggatac gttccattcg
     3181 aatgaccaag tctgttttgt ctaagttcaa ttctttaact tcggtatcac tagctaattg
     3241 cttttgatag gataataatt tagaggaggg ttgtcgtcct tgggagcgta taatttctgt
     3301 gaaacttgtt gttcctcgca ttttttcttg aacacgatga ctggcaacat aagtaccact
     3361 tcctatccgc ctttcaagaa tcccttcttc tactagtaat gtaatagcct gccgtagagt
     3421 cattcgacta acggtgaaat gttccgctaa atgtcgttca cttggtaagc gactaccaat
     3481 tggccagatg cccagatcaa tgtctttttt aattgcatca tgaattttta tataagctgg
     3541 taacatgcca cactcctcaa gtcggttatt catctatttt atcatatttt tgatgaaaat
     3601 tgaattgtaa atattgaggt atttggcatc aattaatgat attacctgcg acaataactg
     3661 gtgaatttgg tggaaaatga ataggttcca taaaccaact agtgagcttc gctttagtaa
     3721 caatggatga ggtaaaaaca ttatttaaaa ccctaataac tcctactttt gaggaatgct
     3781 gaccaataga accactatag gctctcaggc aaccagtcgg atctatataa ggtaaaaatt
     3841 gattttggct tacagtaata ttgtgacata gatgtcgagt ttggttaaat gcttcatagt
     3901 ttggagcaat attactaagt agacctccat cccatgcaaa atgatgaaca gcagcatcaa
     3961 actgaatgac ttctgaaaaa aaatcatgcc cttgagcctt tgatagcagc tgttggtctt
     4021 gtactaattc tggcgcatag ccaataaatt ggtttttctc aaacaatgaa ttttgtagag
     4081 atcctaaatc aaaaatatga ctattacgct tatgaaccat agtaaatttg ttatcgtaaa
     4141 tatgccaatc agtaccatga tcagccataa tcataaagtg gtctcctttt ttcaaatcat
     4201 ttgctttgaa atgcactcca gtcaacacga gattacgaac accttgttca gcctttggtc
     4261 ctgtagggaa tccaaaccac aacattttac cgtgaataat gaactctgtc tgatgaccaa
     4321 taatagctgt atcagatgga agctcaaaat aatctttttg tgaaggcaaa gagccaatgt
     4381 gaaattgccc ctttggaaaa gatataacgc tatttggatg ctgctttgca taggcgaaag
     4441 cttttcgcaa aagacgattg ttttgctcag gggaatttcc tttattgaac ctaatataat
     4501 aaggtttaac aaaatcttgc tctgaaattc catctaatct tagattatct ttcaggttaa
     4561 tatcgtactg atatcctcct aacgaatagt catagcgttt tttaacttgt ttaatagtcg
     4621 ctaattgacc ttgatacact tttatatgtg gctttttagt taatgaagat aatttaacga
     4681 tatctccttt tttaaacctc ggtttatcag gctttttcag gttatgttct ggcaaagaaa
     4741 cagtgacttt tcctattgga aataccactt cataatgata actcactcgg taattttcgg
     4801 ctatatcttc tacctgttta ttaatagttc caatccattg atgatatgga ccactttttt
     4861 gtacttgaat agcttgtcca atgtgatagc gaggctgttg atctttgctt cgttccacta
     4921 ttccgacagt tgctaatata ttatgaagtg ttgcaaaact attggtccac gcaaggtacg
     4981 aaacaactaa actcaacaat aatcctagga aaattaattc aacaggtcga aataaaccca
     5041 aaattctctt ttttcttagc ataggccctc cctgttgtct taattccctt tcatagcctc
     5101 tattgtaaca taacgattac gtttttttcc aacgagctag agaaattgta atgtcttttc
     5161 gatgaaaata tgctacaata agttgacaac tatcagcctg acggttggta tagaatgaaa
     5221 ggattataat gactgaaatt tcaattttga atgatgttca aaaaattatc gttcttgatt
     5281 atggtagcca gtacaatcag cttattgcta gacgtattcg agagtttggt gttttctccg
     5341 aactaaaaag ccataaaatc accgctcaag aacttcatga gatcaatccc ataggtatcg
     5401 ttttatcagg agggcctaac tctgtttacg ctgataacgc ctttggcatt gaccctgaaa
     5461 tctttgaact agggattccg attcttggta tctgttacgg tatgcaatta atcacccata
     5521 aattaggtgg taaagttgtt cctgctggac aagctggtaa tcgtgaatac ggtcagtcaa
     5581 cccttcatct tcgtgaaacg tcaaaattat tttcaggcac acctcaagaa caactcgttt
     5641 tgatgagcca tggtgatgct gttactgaaa ttccagaagg tttccacctt gttggagact
     5701 caaatgactg tccctatgca gctattgaaa atactgagaa aaacctttac ggtattcagt
     5761 tccacccaga agtgagacac tctgtttatg ggaatgacat tcttaaaaac tttgctatat
     5821 caatttgtgg cgcgcgtggt gattggtcaa tggataattt tattgacatg gaaattacta
     5881 aaattcgtga aactgtaggc gatcgtaaag ttcttctagg tctttctggt ggagttgatt
     5941 cttcagttgt tggtgttcta cttcaaaaag ctatcggtga ccaattaact tgtattttcg
     6001 ttgatcacgg tcttcttcgt aaagacgagg gcgatcaagt tatgggaatg cttgggggca
     6061 aatttggcct aaatattatc cgtgtggatg cttcaaaacg tttcttagac cttcttgcag
     6121 acgttgaaga tcctgagaaa aaacgtaaaa ttattggtaa tgaatttgtc tatgtttttg
     6181 atgatgaagc cagcaaatta aaaggtgttg acttccttgc ccaaggaaca ctttatactg
     6241 atatcattga gtcaggaaca gaaactgctc aaaccatcaa atcacatcac aatgtgggtg
     6301 gtctccccga agacatgcag tttgaattga ttgagccctt aaacactctt ttcaaagatg
     6361 aagttcgagc gcttggaatc gctcttggaa tgcctgaaga aattgtttgg cgccaaccat
     6421 ttccaggtcc tggacttgct atccgtgtca tgggagcaat tactgaagaa aaacttgaaa
     6481 ccgttcgcga atcagacgct atccttcgtg aagaaattgc taaggctgga cttgatcgtg
     6541 acgtgtggca atactttaca gttaacacag gtgtccgttc tgtaggcgtc atgggagatg
     6601 gtcgtactta tgattatacc atcgccattc gtgctattac gtctattgat ggtatgacag
     6661 ctgactttgc tcaacttcct tgggatgtct tgaaaaaaat ctcaacacgt atcgtaaatg
     6721 aagttgacca cgttaaccgt atcgtctacg acatcacaag taaaccaccc gcaacagttg
     6781 aatgggaata acagaataaa cattaaaaga ctcgctcatt ttggcgagtc ttttaatgtt
     6841 tattctttaa ctgttttcgt attttgtaca tggtattagc aatatgataa agtgggctga
     6901 cagggagatt aaattctcct ataaattctt ctattgtcgg agcaaagttg gccttaaatt
     6961 tggtaagtcc atcatcaagc gagccttcta ctcctcccat gttagcccaa ataataccat
     7021 cttgataagc atcctgaaaa actttaggat acagcagata ctgaggataa aacttcttaa
     7081 aatcatcatt catcccagca taaagcattt ccataacatt tccataagaa atagataata
     7141 tacctgctac aacaacttcc tcaggatatt ggtctgtaaa gccttcaaat tcagttatat
     7201 aacgttctaa agaagctttt tgatcttcta atttttttaa acgcttcttt tgatgagctt
     7261 gggtgcgagt aatatcttgg ttaataagat ttaattgctg gcggtattga tctagttttt
     7321 gaggaatatt cacttttgct agatgtaagt aggccctatc cccataagta gtcatcaact
     7381 tttgaaaata agcttcgtta cgcaaagaaa tatttttacg cttttctgtt aaggagacaa
     7441 tcttggaaaa tttgtgaagt tctgattgac tggcacgata tgtttctaca ccacgctgct
     7501 tagcatcttt tattaaacgt ctggtatgct taggaaattg catctcaagg ttttcttgag
     7561 tgtaaatatt agcttggaaa cgaggttgga tactatctgc aatctccatt gttaaaccag
     7621 tccaatgaac accagcttct tggagattct taatagctgc taaagctaaa ggtttttctt
     7681 cttcttcctg tcccagtgcg tattgtttta acaggatagc tggatcatat ttgataaaga
     7741 gggccttttt agttttacca taatccttta gtgttttcat ggtaaatgcc accaaatcac
     7801 ggtcaagata atccatgact ggccctcttg gaatataaat aatgcttctt ccaagtggca
     7861 atagcttaat caaaagtgat aaagaggcaa cctgcttttc ctctttatag ataccaattc
     7921 gctcattctg ccattgattt ttgatttttg cccaatcact accttgtaaa acgctaattt
     7981 gctgatgttc tttaacaaaa gagtcatgct cttcttctga aataccgatt tttgcataaa
     8041 acgttaaatt cgtcatttat tgtccccatc tattcgtaat cttagcagat gtacttacta
     8101 atcatcttca ctgtctttat ctatttattc gtaataaatt ttaaaaaaac ataaaaatag
     8161 ggaaactatt tgaatcactt tttagtaata acgtttgtat cttaaatgac ttgttaatca
     8221 ccaacaattt catgaacaac tgatcatcaa aaaataagct attattaaac gactttacac
     8281 cattccatta gataaaaaaa agatgtagtc acatcttttt tggttggtga tcagttacct
     8341 aaaccatcac atatctagca gtatttattc tatatcttta ctgattttag taatttctgt
     8401 gtaaaaaatt aaggaatagt ttaggttaac ttactaaaat cccaagtatc ggttacccac
     8461 ccattgtaga aatcaggttc atgacaaacc attaaaatag aagccttata tgcttttaaa
     8521 gcacgtttga gctcattttt agcatcaata tcaagatgat ttgttggttc gtctaaaata
     8581 agcacgttat tttcacgatt catcaacaaa caaaaacgaa cttttgcttg ttcaccaccc
     8641 gaaagtactt gaatttgact ttcgatatgt tttgatgtta gtccgcaacg agctagtgcc
     8701 gctcgaactt ctgcctgatt taaggcagga aaagcatccc aaactacttc tagcggagtt
     8761 tgtcggttaa tacctgtcac ttcttgttca aagtagccaa cttctaaaaa atcccctgtg
     8821 acaatatgac cttctaaagg ctcgataaca cctaataaac tttttagcaa agtagatttt
     8881 ccaataccgt tggccccaac aatagcaatt ttttggtttc tttcaaacgt tatatttaag
     8941 ggttctttgg tcaatgggta atcataacca atcacaagat tttttgtttg aaaaatgaat
     9001 cgactgggag ttctagcttg cttaaattca aaatttggtt ttggtttctc agcttgaagt
     9061 tcaattatat ccatcttatc aagttttttt ggcgagacat tgccatgtta cgtgtcgcta
     9121 cccgagcctt atttcggttg acaaaatcct gcaagttagc aatctctttt tgttgacgtt
     9181 cataggctgc ttcaagttga gattgtttca tctcataaac agcttgaaat tggtaataat
     9241 ccccagtata gcgaactaaa ctttgatttt caacatggta aacaatatta atcacatcat
     9301 ttaagaaaga aatatcatgc gaaatcaaca caaaagcatt ttcataatgc tgtaaatagc
     9361 gttttaacca ttcaatatgc tcagcatcca aatggttagt tggttcatct aatagcagaa
     9421 tatcaggttt ttctaatagt aatttggcta ataaaacctt tgttcgttgc ccacctgata
     9481 aggatgtaac atctgactcc attccaaaat ccataacacc aagcgcacgc gctacttcat
     9541 caatcttagc atccaaagta tagaaatcac gactttctaa acgatcttga agctcaccta
     9601 cttcttccat caaaacagca atatcagctt tatcatctgc cattgacgcg taaatctcat
     9661 taatacgatt ctcggtctta aataactcat caaaagcagt tcgcaagaca tcacgaacgg
     9721 tttgtcctga ttccaacact gtatgttgat ccaggtaacc tgcagtgaca tacttcgacc
     9781 actctacttt tccttcatca ggctgtaaat gtcctgtgac tatactcata aaggttgatt
     9841 ttccttcacc atttgcccca actagtccaa tatgttcgcc ttttaagagg cgaaatgaca
     9901 cattttcaaa aatagcccga tccccaaaac cgtgactcaa ctgcttaact tctaaaatac
     9961 tcatcgtttc ttccttaact gtcatcaaaa aagccgtcaa aacggcttta tagttatttc
    10021 attatggcaa aattgtaacc ttttgtcaat atttgactaa aagtgtacct tgctttttta
    10081 tcgataaggt acttgtatca aaaacctctt tttgcttgtc cgattgtaac cttttctttt
    10141 aagtttttga taacaggata caaaagagga acagcaacta aagtcacaaa agatgttccg
    10201 aaactaccac caatttttcc aagagctacc caaaaagata ctgaaagtgg tagctgagta
    10261 cctatataag ctaacagtaa gtattttata aggttaatag ttaattttga caatgcagca
    10321 ataactgtta ttgttataat ggcttttggt tttttactag attgcatagc tctttcatat
    10381 accaaatgca aaattaaaca aatagccaag gattctaaga tagttatcca aacgactgaa
    10441 gcatagccgt tcaaaacatc aaagatccct aaaccaagac tagcaactag agctcctttt
    10501 ttactaccat agattaagac tgcaataaca actaatgcat tgccaagatg aataaactgc
    10561 ataccgacag ggatacgcaa cacttgtact gaaataaaaa ttaaagcggc atacatactc
    10621 aattcaatat aattagctaa tttttgagat gacatttttt tcctccaaca actttttaaa
    10681 cgatttagtt aagtaattta aatgagattc ataagcaaca cctaagatta aatctctttt
    10741 taatgtaagg gtattttgca atactttttc aagaaatgaa acaacaagat ctagaatttg
    10801 tgttagtggt agctgataat agaatcctgc tgttataata gctgatgcta tatctcctgt
    10861 accatagaaa tggtaaggga attgcttccc aaaatggaat gttaattgat ctgaatctga
    10921 ctgatagtaa gcaaaaccaa tgctgtcctc ctcaaaagag attccgctaa tgacaacctg
    10981 tttaggtcca agcttagcta attttctagc cacctgtcta atatcttctt catcataatt
    11041 ttttgctaaa taagctgttt gtgttaatag acaagcctca ctaatattag gtaaaacaag
    11101 ctcagctttt tgagtaagtt ttatcataat ctctatatgt ttttgactga agccttgata
    11161 aagttttcca ttatctgcca taattggatc taaaattaag ggaagttgct ttcttgaaag
    11221 ccattctagc agcttttgcc catctcttgt tgtcgtaaaa taacctataa agatgccgtc
    11281 aaacttaatt cttttgttgt cccaatctgt taaaaaaacg tctaatatac ttgatgtagt
    11341 atgcttcaca gggctctgtc ctccgccagt atggcttgat aaaaggcaag ttggtaataa
    11401 aacttgctct aacaaacaag aagccattaa aggaatacta gcagacaagg ctaccttacc
    11461 aacaccaacc agatcatttg ccacaacaat ccgtttcata gctattacct cttttctata
    11521 ataactattg taagaaaaag gggcataaga tacaatagtt ataacactta actgtatggg
    11581 gacaatttat gacaacaaaa tatcaaacaa tcatttccaa tatcgaacaa gatatccaga
    11641 aacagcgact aaaaaaaggg gacaagctac cttcaattag ggtattaagt aaggtttatc
    11701 attgtagcaa agatactgtt cagcgggctc ttttagaatt aaaatatcgt cacttaattt
    11761 atgctgttcc taaaagtggt tattatgttt tgggtaacgt aagcatgccc gataatgttc
    11821 ttaatctaag tcttgaagat tataataata tggcttatga agatttccga ctctgcttaa
    11881 atgaggcttt aagtgctaaa gacaaatacc tctttcatta ttatcacaaa accgaaggac
    11941 tagaagaact aagggaagct ttgcttcttt acttagctga aaatagtgtt tacagtaaca
    12001 aagaccaact attaatcacc tctgggacgc aacaagcgct ttatatttta tcacaaatgc
    12061 cttttcctaa tgcgggcaaa acaattctcc ttgaaaagcc tacttatcat cggatggaag
    12121 ctattgttgc tcagttagga ttaccctatc aaactatctc tagacacttt aatggccttg
    12181 atttagaact cttagaatct ttatttcaaa ctggcgatat taaatttttt tacacgattt
    12241 cacgattttc acatcctctg ggactttctt atagcactaa ggaaaaggaa gctattgttc
    12301 gtctagcaca acgataccag gtttatattt tagaagatga ttatttagga gattttgtga
    12361 aacttaaaga gccaccaatt cattattatg acactcatca ccgaatcatc tatctaaaat
    12421 ctttttcaat gagtgttttt cctgcactcc gtatcggagc tctagtttta ccgtcaggtt
    12481 taaaacctca ttttttaacc caaaaatcat tgattgattt agacacgaac ttactaatgc
    12541 aaaaagcatt agctctttat ttagaaaatg ggatgtttca aaaaaatctt cgctttatta
    12601 agcgttatct aaaacagcgg gaacgtcaat tggctctctt tttaaaacaa aattgccctg
    12661 atattcacta tcaactaacc cctactcatt tagtgattga tt
//
LOCUS       DAUCWO010000049        12526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUCWO010000049 DAUCWO010000000
VERSION     DAUCWO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.049"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            354..764
                     /locus_tag="VGY20_001297"
     CDS             354..764
                     /locus_tag="VGY20_001297"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ4274147.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(846..2858)
                     /locus_tag="VGY20_001298"
     CDS             complement(846..2858)
                     /locus_tag="VGY20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ4274148.1"
                     /translation="MKKYFILKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGAL
                     DNTGTAYTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANS
                     ALLQDEPTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEH
                     EASHQTIVIANVIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQ
                     NYEHYQFLDVAETIAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQI
                     YPEHSIDIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSAN
                     VVAVAPGIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNL
                     VTTAQLTIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQM
                     TGQHIYDVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLT
                     TTYTVVVNDFLYGGGDGFSAFKKTKLIGAINTDTEAFITYITNLEASGKTVNATIKGV
                     KNYVTSNLESSTKVNSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKE
                     TTTNKNTISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKERKS"
     gene            3078..3728
                     /locus_tag="VGY20_001299"
     CDS             3078..3728
                     /locus_tag="VGY20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ4274149.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            3731..4399
                     /locus_tag="VGY20_001300"
     CDS             3731..4399
                     /locus_tag="VGY20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4274150.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            4408..5640
                     /locus_tag="VGY20_001301"
     CDS             4408..5640
                     /locus_tag="VGY20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4274151.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPDTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            5933..6811
                     /gene="mvk"
                     /locus_tag="VGY20_001302"
     CDS             5933..6811
                     /gene="mvk"
                     /locus_tag="VGY20_001302"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ4274152.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            6793..7737
                     /gene="mvaD"
                     /locus_tag="VGY20_001303"
     CDS             6793..7737
                     /gene="mvaD"
                     /locus_tag="VGY20_001303"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ4274153.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDQWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            7730..8743
                     /locus_tag="VGY20_001304"
     CDS             7730..8743
                     /locus_tag="VGY20_001304"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ4274154.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFAHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPTIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            8730..9719
                     /locus_tag="VGY20_001305"
     CDS             8730..9719
                     /locus_tag="VGY20_001305"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ4274155.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(9949..11226)
                     /locus_tag="VGY20_001306"
     CDS             complement(9949..11226)
                     /locus_tag="VGY20_001306"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ4274156.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(11213..12388)
                     /locus_tag="VGY20_001307"
     CDS             complement(11213..12388)
                     /locus_tag="VGY20_001307"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ4274157.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APVTEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIHLTGKFALTAIKEHRRIYHTNDKN"
BASE COUNT         3924 a         2211 c         2490 g         3901 t
ORIGIN      
        1 ttaaagcatt tcaaacagca ggtgttttat ttggacctgt taaggctgca aatgcaagag
       61 aaagcagtat ctggtttgaa aatctcacaa agcaataact atctctctta gacatttgaa
      121 gaagcagaca gtaaattcga taatattgtg atgagaactt agcaacaatc tgctgatgca
      181 ggtcaatttt tatttggtac tcatattgca gaatttcata aagttgccca agatatcttg
      241 aaataaggaa tcacctgatg ttttaaacag atgatactaa gtctatcata tggtgataat
      301 agtccattga acatgtagtt tatgaattaa aaagaaggtt taaggagata agaatgatta
      361 gagaaatcat aacagatcat tttttattac agcaaaaagc gcaagtcgcc aagaaagaag
      421 atctatggat tggccaagat ttacaagata ccttagcctt ttatagacag gaatgtcttg
      481 ggatggctgc caatatgatt ggtgaacaaa agcgcattgt tattgtgagt atgggattta
      541 ttgatttggt tatgtttaat cctgtgatgg tttctaaaaa gggaatttac caaactaaag
      601 aatcttgttt gtcgttatct ggctatcgca aaacacagcg gtatgataag attactgttg
      661 agtatttgga ccataattgg cgaccaaagc gattaagctt aacaggctta actgctcaaa
      721 tatgccagca cgaattagat catctagaag gtattttaat ttaacaaaaa acctagagta
      781 ggacgcttta ggtttttttg gcaaagatgc ctgattatat aagggtgatc gattactagt
      841 aaacgctagg atttcctttc tttaataaaa gcgtttagtc cacctaagct tatcaaggca
      901 aggattgtca taataggaga cattttataa ttgtcccctg ttattggaag agtggaacta
      961 gagatagtat ttttgtttgt cgttgtttct tttttgccaa ggctgttatt agtgttttca
     1021 gtagaagtca aaaggtctga aatgatttca ctagacacta tattgccatc acgatttcta
     1081 aaaaccttac taatgatact gtgtttacca gcactattaa cttttgtcga gctttcaagg
     1141 tttgaagtta cataattttt aacccctttt atagtagcat taacagtttt acctgatgct
     1201 tctaaatttg tgatatatgt gatgaaagct tcagtatctg tgttaatagc tccgattaat
     1261 ttagtttttt taaatgctga aaagccatca ccaccaccat aaagaaagtc gttgacaaca
     1321 acggtgtaag tagttgttaa gttaatttct tcaccattgt ctttataaac cttaactatc
     1381 ttgaaggggg tatcagagtt cttaggatca ttatctgtat aagtgtatgt taaacctgac
     1441 atttgaagaa aataggtctg gttttcatcg tattgctgat ttaggacatc gtaaatgtgt
     1501 tgaccagtca tttgaatgac ttgaaggata ttaccaaatg gttgtacagc ctgtgcagct
     1561 ccccaggtga tggtccggtc atttttgaca actaggtcac ttcgaatacc accattattg
     1621 gtcatagcaa agtcaacagt tggaaaagtt ttcttagcaa tagtaagctg agccgttgtt
     1681 actaagttac cgacaggaga ttctttatca atattttctg ttttagaaat agttgaagaa
     1741 ttagttgcag ttccgatttt tcgttcagta actgttttaa caatatcatt agcatgattt
     1801 attatagctt tgatatctga attttctgtt ttgatacctg gtgctacagc aacaacattt
     1861 gctgatggag ttttaataaa atcattggta tcagtatcta gcgtaccacg gacatctgca
     1921 taagcttttc cttgagagag ggcttgaacg atacgtgttt taccgatagt tccattagtg
     1981 tattgatgat tatgtcctgc aaaaataata tcaatgctat gttcgggata gatttggttc
     2041 actttttcca taaccgtagc catttcatga tcaacaacac catctttact tgttgcagga
     2101 atatgagcta aaaccacaat agcatgaaca tgttgttctt gcagttcttt agcatatttg
     2161 gcaatggttt cagctacatc taaaaattga tagtgttcat agttttgctt taaaacgaga
     2221 tttggaatct ctgtagtcac aacaccaatg aagccaatct taacgatttt gtcattaatg
     2281 gctatgtctt ttatagcata aggtttccaa ccatagggga tatcattagt ttttttatca
     2341 atgacattag caatgacgat ggtttgatgc gaagcttcgt gctcatattg tttggtgata
     2401 tcgttaattg ttgattcagg atcaggcgct tgacctgtca tgatacggtt aaattcatct
     2461 agtccttcgt caaattcatg attaccaaga gtgccatatt caaatttcat tttgttaaag
     2521 actttcacag taggctcatc ttgtaaaagt gcagagttag caggactggc tccgaccata
     2581 tctccagctt gaacacgtat acttgttccg tcttgatttg cttgcttgaa atctatctca
     2641 gcgtcatcca tataagcacc taattgagca gccgtcccag catttggtat tttaccactt
     2701 ggtgtgtaag ctgttccggt attatcaaga gcgccgtgaa aatcattgac gccaaggaat
     2761 tgcacatcaa cttgatctgc ttgaacgtct gtaactaata gagtaaaact agtcaggata
     2821 ctcaatacac tactttttaa aataaaatat tttttcataa aatctcctaa taagttaacg
     2881 taatcgttat agatcaagtt tactatgttt ttgtttgtaa ttcaatggta tttttaaaaa
     2941 ataacgtaca aaaaatatat gaaagcgatt taaaagcaaa cgatacagat catttgcagt
     3001 tatatagtta gaaataaaga taaccgatca ttgcagagat ttaagcattt gttgagaaaa
     3061 tttgttataa taagcgtatg aaagaaagac tagaacatta ttctaccagc tctatatata
     3121 gcggatttga agtgtacttg ccgttggtac tacagacaat aacagatgtg attattgctg
     3181 agaatatcaa aagcaaaaaa gaaacagggt ttaaactata cgaacacttt acatcccgta
     3241 ttaaatcgga agcttcgatg attgaaaaat gtcaacgcaa acagcttcct ctaacaagca
     3301 aatctgcgtt aaaaatcatt aaagatagta tcggtattcg cattatttgt ggttttattg
     3361 atgatattta tcggatggta gacttgctca aaagcattcc tggtatgtca gttaacactg
     3421 aaaaagatta tattttaaat gccaagccta atggctatag atcatatcat ctcattttgg
     3481 aattagaaac acattttcca gatattttag gggaaaaaaa aggatgttat tttatagaag
     3541 tgcagttgcg aaccattgct caagactctt gggctagctt ggaacatcaa atgaagtaca
     3601 aacatcaagt tgctaatgca gaaatgatta caagagagtt aaagcgttgt gcagacgagt
     3661 tggcatcttg tgatgttacc atgcaaacca ttcgtcaatt gattcaagaa acgacggagg
     3721 aagagtaacc atgaaaattt tattagctga agacgaatgg cagatgtcta atgttttgac
     3781 aactgccatg actcatcaag gttatgacgt tgatgtggtt tttaacggtc aagaggcaat
     3841 tgataaagcc aaagacaatg catatgatat tatgatttta gatatcatga tgccgattaa
     3901 gtctggcatt gaggctttaa aggagattcg cgcttcagga aattgctcac atattattat
     3961 gctgacggct atggcagaaa ttaatgaccg tgttaccgga ctagatgcgg gagctgatga
     4021 ttatctgact aaacctttct cattaaaaga acttttagct agattaaggt cgatggaaag
     4081 acgagtagaa agttttactc ctcaggtatt acaatttgct ggtgtcacat taaatattaa
     4141 tgagcaagaa ctgagtgccg gtaatgctat acgattagct tctaaagaag gtaaattaat
     4201 ggccttttta atgttaaatc aaggaaagta cttggatact aaaacacttt accaacatgt
     4261 ttggtcagac caagaagatt atgatacttc tattgtttgg gtttatattt cttatcttcg
     4321 tcaaaaatta ttagctattc aagcaaatgt cattattgca ggtgataaag atactagcta
     4381 ttgtttatta gaaaaatagg aggtgacatg tttaatcgta tccgtattcg ttttatcatg
     4441 attgcttcta ttgccatttt tattatttta agttcaattg ttggtattat taacacggca
     4501 cgctgttacc aaagtcaaca agaaattaac cgtattttac atttaatttc ctcaaataaa
     4561 ggtaaattac ctgacactac ggaaagctct aaacgacttg gaacgaaact gtctgaagat
     4621 agcctaagcc agtttcggta ttatagtgtc atttttaatg ctaatggaca tctactttct
     4681 tctaatactg ctaatatttc agctttagat agagaagaag cccaatattt tgctagactt
     4741 tttgctaagt ctggggaaga aaaaggcagt taccgtcacc aagatagtgt ttattcgtac
     4801 ttgattactc agctgccaaa tgaagaaaaa ctggttgtta ttttagatac taccttttat
     4861 tttcgtagtg taggagattt attagctgtt tcaatgatgt tggcctttgg aggatttatc
     4921 ttttttgttg ttttagtgag tcttttttcc ggtatggtca tcaaaccttt tgtccaaaat
     4981 tatgagaagc agcgccgctt tattactaat gcaggccacg agttaaaaac accattagcc
     5041 attatctcag ccaataatga attggtcgaa ttaatgactg gagaatcaga atggaccaag
     5101 agtacaagtg atcaggtgaa gcgattgaca ggcttgatta atcaaatgat tactttggct
     5161 cgtttggagg aacagccaga tgtggtatta catatggtag acttttcagc tattgctcaa
     5221 gatgcagctg aagattttaa gagtctggtt ttgaaagatg gtaaacgttt tgatttgacg
     5281 attcagccga atattatgat taaagcggag gaaaagtcat tatttgaact tgtgacaatt
     5341 ttagttgata atgccaataa atattgtgat cctaaaggct tggtcaaggt atctttaacc
     5401 actattggaa gaagacgaaa acgagcgaaa ttagaagttt ctaataccta tttggaagga
     5461 aaatccattg attacagccg cttttttgaa cgcttttatc gtgaagacga atcccataat
     5521 agcaaagaaa aaggctatgg aattggttta tctatggcag aaagtatggt taagttattt
     5581 aaaggaacga taactgtaaa ttataaaaac gatgctattg tttttacagt ggtgatttaa
     5641 cttaaacatt tttcagtacc tttagctagt aataatgtct aggcaaaggt aataaatgat
     5701 gcttaaccag cccatgaggc tggttttaac gtttaattta gtagaagagt gattaaagaa
     5761 ggtagtttga aataagaaaa tttttgttat gatcctaaaa agagtagata gtctttattg
     5821 ttaatttcag tgaagaaatg attttaaaaa gatatatcat cgcattttct aatcatttag
     5881 tgtaaaatag taaggtaaaa gtttagaaga attagtaatg aaaagataaa aaatgaatga
     5941 aaacattgga tatggtaagg cacacagtaa gatcattttg ataggtgagc atgctgttgt
     6001 gtatggctac ccagctattg ctttgccttt aacagatatt gaggtggttt gtcatatttt
     6061 tccagctgat aagccattgg tctttgattt ttatgatact ctatcaacag ccatttatgc
     6121 tgccttggat tatttgcagc gactacaaga accaattgct tatgagattg tttcacaagt
     6181 gccacaaaag cgtggtatgg ggtcttcggc agccgtttct attgcagcta ttagagccgt
     6241 tttttcttat tgtcaagaac ccctctcgga tgatttgttg gaaattttag tcaacaaggc
     6301 agaaattatt gcccacacca atcctagtgg tttagatgcc aagacatgcc ttagtgacca
     6361 tgccattaag tttatccgaa acattggctt tgaaactatc gaaatcgcct taaatggtta
     6421 tcttgtcatt gcagatacag ggattcacgg tcatacacgt gaggccgtca ataaggtagc
     6481 acagtttgag gaaacgaatt tgccctatct ggctaaacta ggagctttga cacaagccct
     6541 tgaaagagcg attaaccaaa aaaataaggt ggctatcggt cagctcatga cacaagcgca
     6601 ctctgcttta aaggccattg gcgttagcat cagtaaagct gaccaacttg tggaggctgc
     6661 cctaagagca ggtgctttag gcgctaagat gacaggcggt ggcttgggtg gctgtatgat
     6721 tgccctagca gataccaaag acatggccga aaaaatcagt cacagattaa aagaagaagg
     6781 agccgtaaac acgtggatcc aaatgttata accgtaacat cctatgctaa cattgctatt
     6841 atcaaatatt ggggaaaaga gaatcaagct aagatgattc cttcgacctc tagcatttct
     6901 ttgactttgg aaaacatgtt caccacaacc agcgtttcct tcttaccaga tactgcaacc
     6961 agtgatcagt tttacattaa cggtgtcttg caaaatgacg aagaacatac caaaatttct
     7021 gctatcattg accaatttcg ccaacctggt caggcttttg taaagatgga aactcaaaat
     7081 aatatgccaa cagctgcagg tttatcatca agttccagtg gcttatcagc cttggttaaa
     7141 gcctgtgatc aattatttga cacccagcta gatcagaaag ctttagctca aaaggctaag
     7201 ttcgcctcag gatcatcttc tcgttctttt tttggcccag tcgctgcttg ggacaaagat
     7261 agtggtgcta tttacaaggt tgagactgac ttgaaaatgg ccatgattat gctggtctta
     7321 aatgctgcga aaaagccaat ttctagccga gagggcatga agttatgccg cgatacctca
     7381 actacatttg atcaatgggt agaacaatcg gcaatcgatt accaacatat gcttacctat
     7441 ctcaaaacta ataattttga gaaagttggt cagttaacag aagctaatgc cttggccatg
     7501 catgccacga caaagacagc caatcctccc ttttcttacc taacaaaaga gtcttaccag
     7561 gctatggagg ctgtgaaaga actgcgtcaa gaaggttttg cttgttattt taccatggac
     7621 gcaggtccaa atgtgaaggt cctatgttta gaaaaagact tggctcaact agcagaacga
     7681 cttggcaaga actaccgtat tatcgtttca aaaacaaagg atttaccaga tgtctaatta
     7741 ttgtgtgcaa acaggtggga aactatacct cacaggcgaa tatgctatct taacaccagg
     7801 acaaaaagcc ttaattcact ttattccact gatgatgaca gcagaaatta gcccagcagc
     7861 ccatattcaa ttagcttcag atatgtttgc ccataaagcg ggcatgacac ccgatgcctc
     7921 ttatgcactg attcaagcaa cggttaaaac ctttgctgat tatctaggac agtcaattga
     7981 ccaactggag ccattttccc taattataac gggaaaaatg gagcgcgatg gcaaaaaatt
     8041 tggcattggt tcaagtggta gtgtcaccct cttaacctta aaggccttat cggcctatta
     8101 tcagatcact ttaaccccag agttactctt taaactagcg gcttatacct tgcttaagca
     8161 aggagataat gggtctatgg gggatattgc ctgtatcgct taccagactt tagtcgccta
     8221 tacctccttt gaccgagaac aggtcagtaa ctggctgcag accatgccct taaaaaaact
     8281 tctcgtcaaa gattggggtt accatatcca agtcattcaa ccagccctgc cttgtgactt
     8341 tttggttggc tggactaaaa tacctgctat ttcaaggcag atgattcaac aagtgacagc
     8401 gagcattacc ccagctttct taagaacaag ttaccagcta acgcaatcag ctatggtagc
     8461 tttgcaagaa ggtcataagg aagaactcaa gaaaagttta gcaggagcaa gtcatctcct
     8521 aaaagagctt catccgacta tctaccatcc taagctagta accttggtag ctgcttgtca
     8581 gaagcaagat gctgttgcta aatcttcagg ttctggtggt ggagattgtg gcatcgcgct
     8641 tgcctttaat cagaatgcca gagataccct tatttccaaa tggcaagaag ctgatatcgc
     8701 attactttat caagaaaggt ggggagagaa tgaccaaccg taaagatgat cacatcaaat
     8761 atgccctcaa gtaccaatcg ccttataatg cttttgatga catagaactc atacatcatt
     8821 ccttacctag ctatgatttg tctgatattg atctcagtac tcattttgcc gggcaagact
     8881 tcgactttcc cttttacatc aatgccatga caggaggaag tcaaaaaggc aaagctgtca
     8941 atgaaaaatt ggccaaagta gcagcagcaa cagggattgt catggtgaca gggtcttata
     9001 gcgctgcttt aaaaaatcct aacgacgatt cctatcgttt acatgaggtg gcagataact
     9061 tgaaactagc cacgaatatt ggtctagata aacctgtggc gctaggacaa caaacggttc
     9121 aagaaatgca gcccctcttt ttacaggttc atgtgaatgt gatgcaagag ttgctgatgc
     9181 cagagggtga gcgcgtcttt catacctgga aaaaacacct cgctgaatac gctagtcaaa
     9241 taccagttcc tgtcattctc aaagaagttg gttttggcat ggatgtcaat agtatcaagc
     9301 tagcacatga cctaggcatt caaacctttg atatttcagg aagaggaggg acctcatttg
     9361 cctatattga aaatcaaaga ggaggagacc gctcttactt aaacgattgg ggacaaacca
     9421 ccgttcagtg cttactgaat gcacaaggac tgatggacca agtggaaatc ttagcttcgg
     9481 gtggtgtcag acaccccttg gacatgatta aatgtttcgt cctaggagca cgtgcagtgg
     9541 gactctcacg caccgtttta gaattggtcg aaaaataccc aaccgagcgt gtgattgcta
     9601 tcgttaacgg ctggaaagaa gaattaaaaa tcattatgtg tgctcttgac tgtaaaacta
     9661 ttaaagaatt aaagggagtc gactacttac tatatggacg cttgcagcag gtcaattagg
     9721 cttcatcacc tctacaatag tgtcacctac tacaaagatg atagagcttt tttagaacat
     9781 aatataaaat atttataaat acctcatcaa actcagaata gagtagatga gtaaatagag
     9841 tatgttatgg agactatttc taagacttgt atgtatagaa acaaagatta aacacccatg
     9901 taaaccaatt gtttacatgg gtgtttgcct tatattagtt gtttaaatct aagactgtct
     9961 tagctgagat aagtaaaaat gtgctttttc tagatttatt ggtttatcag ctagtaaagc
    10021 attaactaaa ggagcaattt cgtcttgggt tgcccccgcc aaaagagcta atgatttcgc
    10081 atgtagtttc atgtgaccag cttggatgcc agatgaggtc agagctttta gtgcagcaaa
    10141 gttttgagct aaccccacag atgcaatcaa ttgggctaat gttttggcat caggttgatt
    10201 aagaagatca tgtgcgatag aaacagtagg gttaagcccg atagatcccc ccttactagc
    10261 aataggcata gggagggtca tttggccaag cagaacttgt ttatcttggt caaaatgcca
    10321 ctgactaagt ccttgatagc taccatttct tgaggcataa gcatgggccc ctgcttcaat
    10381 agcacgccag tcatttcctg tggctattac cactgcatca atgccattaa aaataccttt
    10441 attatgagta gtagcccggt aaggatctac ttgagctagt cggctggcta gctctatttt
    10501 ttgagctaac tgtagggatt ttgttttatc gtgatctaaa aggcgcacgg gaatctcaca
    10561 actagtagta acaagcgact ctgttgcgta attagataaa atcgccatta gacaatgccc
    10621 cttagacagt cttgttaaat caggaacaag agcttccatc atagtattga ccatatttgc
    10681 tcccatagct tcttgggtat caacagtcag atagaaaatc aaaaactctc ctttttcttc
    10741 caaatgaatg gttctagcgc ctccacctct tttaacaatg gaaggataag ctttatttgc
    10801 caatgcaatt agcttttttg ttttatgaag gatggcggct ttagcgttat ctatttggtc
    10861 gatatcgtaa agaacaattt gaccaatcat ttgtcggttt agggtttgag ctttaaaacc
    10921 acctgagcgt ttgattagtt ttgctgcgaa agatgctgca gcaacaacag aaggttcttc
    10981 cgtgacaaaa ggcatctgat aagttgaacc gttgacaaga aaatcaggag ctatgctaaa
    11041 gggcaaagcc aatcgtccta agacattttc agtcatttga ttagctgttt ggataggcaa
    11101 aagaacgtct gttttgagtt gatttaagtt ttcagcatta agtagtttaa atttttcgat
    11161 aagttggagg cgttcttcga atgttttctt tgaaaaaccg ctccagttaa gattagtttt
    11221 tgtcattggt atgatagatc cttcgatgct ccttgatggc tgttagagca aattttccag
    11281 ttaagtgaat gtcaaaattg gcattaccat tatcatctaa ttggacttgt tcgtagaaaa
    11341 ggtcttcgta tttagaaacg gaaagacgtg ttcgttgatc cagtaaagct tgtcgattag
    11401 tcattaacat tttatcgtac ccagcaacta actgaccgga ataaaattca cttacggcac
    11461 cggaaccata gctaaaaaaa ccaattttat ctccagattg taatacttta ctattttcaa
    11521 gtaaagataa caatcctaga taaagtgagc cagtataaat atttccaatt tgtttgctat
    11581 aagtaataga agcttgaaag gcttctatta gtttttctct gtgttcagga ggtactgtgt
    11641 tatccataat gttatttaga ccttttaggg ctaacttggg aaatggaata tggaaacaaa
    11701 ctgcagcaaa atcagataac tgaaggtttt ctcttttctg ataagcttgc cacgtggttt
    11761 ctaagcaatt gagatattgc ttggtagagt aaataccatc aacataaggt gtgaaactat
    11821 agttaggcct ccaaaagtcc ataatatccc tagtttgagc cacattatct ttatttagag
    11881 caagaatacg agggttagca gttaccaaaa gagcaatact accagagcct tgagtcgatt
    11941 ccccaggaga tcctacaccg tatctagcaa tatcactagc aataacaagg acacgagaat
    12001 ctggcttaga agccacatgc agtttagcat agtctagagc agcagttgcg ctatagcagg
    12061 cttgttttac ttcaaaggaa cgtgcaaaag gctggatacc aactaagtga tgtacataga
    12121 tagctgatgc ctttgactga tcagtactcg attcagttgc caaaataacc atatcaatct
    12181 tagctcgatc ttcgtcggtt aagatttgat ctgctgcaga agcagctaaa gtaataatgt
    12241 cttcagtgac tggtgccaca ctaaaagact caatgagtag cccttgacta aattttgctg
    12301 gatccacttg gcgtgcaagc gctaaatctt ccaatttcaa gacatattga ctggttgcaa
    12361 aaccaatctt atcaattcca attgtcatat ttgtccttct tttagttatt tttatagtct
    12421 tacccattct atcacatttt actgcaaaat tagactgata aagttatttg ttttatcctt
    12481 taatagttaa aatgttcatg accacatgaa gtttttcgaa tgttta
//
LOCUS       DAUCWO010000050        12285 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUCWO010000050 DAUCWO010000000
VERSION     DAUCWO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12285
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.050"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..481
                     /gene="lepB"
                     /locus_tag="VGY20_001308"
     CDS             <1..481
                     /gene="lepB"
                     /locus_tag="VGY20_001308"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4274158.1"
                     /translation="VFSTFKVSPETANTYLKSGDLVTIKKNIQPKYKDFVVYRVGKKD
                     YVSRVIAVEGDSVTYMDDIFYLNNMVESQAYLEKMKAHYLNHAPFGTLYTDDFTVATI
                     TADKYQKVPKGKYLLLNDNRKNTNDSRRFGLINASQIKGLVTFRVLPLSDFGFVEVE"
     gene            657..2471
                     /gene="glmS"
                     /locus_tag="VGY20_001309"
     CDS             657..2471
                     /gene="glmS"
                     /locus_tag="VGY20_001309"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ4274159.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSRESTYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLTTTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2667..3002
                     /locus_tag="VGY20_001310"
     CDS             2667..3002
                     /locus_tag="VGY20_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ4274160.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            3109..3750
                     /locus_tag="VGY20_001311"
     CDS             3109..3750
                     /locus_tag="VGY20_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4274161.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3760..4389
                     /locus_tag="VGY20_001312"
     CDS             3760..4389
                     /locus_tag="VGY20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274162.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            4405..5241
                     /locus_tag="VGY20_001313"
     CDS             4405..5241
                     /locus_tag="VGY20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4274163.1"
                     /translation="MIIKKRTVAILAIASSFFLVGCQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
     gene            5611..6384
                     /gene="cfa"
                     /locus_tag="VGY20_001314"
     CDS             5611..6384
                     /gene="cfa"
                     /locus_tag="VGY20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ4274164.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTYSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
     gene            complement(6754..7989)
                     /locus_tag="VGY20_001315"
     CDS             complement(6754..7989)
                     /locus_tag="VGY20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ4274165.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLSLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAVVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEELFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TFNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQRTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(8109..9431)
                     /locus_tag="VGY20_001316"
     CDS             complement(8109..9431)
                     /locus_tag="VGY20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ4274166.1"
                     /translation="MIAFVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(9559..9645)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9960..12278
                     /gene="pcrA"
                     /locus_tag="VGY20_001317"
     CDS             9960..12278
                     /gene="pcrA"
                     /locus_tag="VGY20_001317"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ4274167.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         4006 a         2155 c         2475 g         3649 t
ORIGIN      
        1 tgttttttca acttttaaag tttctccaga aacagctaat acttatttaa agagtggtga
       61 tttagtcaca atcaaaaaaa atattcagcc caaatataaa gattttgtgg tttatagagt
      121 tggaaaaaaa gattatgtca gtcgagtcat tgctgttgaa ggcgatagcg tgacttatat
      181 ggatgatatt ttttacctca ataatatggt agagtcacag gcttaccttg aaaagatgaa
      241 agcacattac ttgaatcatg caccgtttgg cacattgtat acagatgatt ttacagttgc
      301 taccatcaca gctgataagt atcaaaaagt tcctaagggg aagtatcttc ttttgaatga
      361 taaccggaaa aatacgaatg acagtcgtcg atttgggtta ataaatgcct cgcagattaa
      421 aggtttagtg acctttagag ttttgcctct cagcgatttt ggatttgtag aagtagagta
      481 gttgtcaggc tactctactt tttttataat tagactatac caattttatg tttgacaaga
      541 cattgtggat attatatact gtaaacatac tgtttttatc ggtataatta gactatacca
      601 attattgaaa ttatgagaga aagtagcaag ttaacgaact tgttaaggta attaagatgt
      661 gtggaattgt tggagttgtt ggaaatcgca atgcaacgga tattttaatg caaggccttg
      721 aaaagcttga ataccggggt tatgattcag caggaatttt tgtggctaat gccaatcaaa
      781 caaacttgat taaatcagtg gggcggattg ctgatttgcg tgccaagatt ggcattgatg
      841 ttgctggttc aacagggatt ggtcacaccc gttgggcaac gcatggccaa tcaacagagg
      901 ataatgccca tcctcacacg tcacaaactg gacgttttgt acttgttcat aatggtgtga
      961 ttgaaaatta ccttcacatt aaaacagagt tcctagctgg acatgatttt aaggggcaga
     1021 cagatactga gattgcagta cacttgattg gaaaatttgt ggaagaagac aagttgtcag
     1081 tactggaagc ttttaaaaaa gctttaagca ttattgaagg ttcctatgcc tttgcgttaa
     1141 tggatagcca agcaactgat actatttatg tggctaaaaa caaatctcca ttgttgattg
     1201 gacttggtga aggttacaac atggtttgtt cagatgccat ggccatgatt cgtgaaacca
     1261 gtgaatttat ggaaattcat gataaggagc tagttatttt aaccaaagat aaggtaactg
     1321 ttacagacta cgatggtaaa gagctgatac gagattccta cactgctgaa ttagacttat
     1381 ctgatattgg caaagggact tatcctttct atatgctgaa agaaattgat gagcaaccaa
     1441 ccgtaatgcg tcaattaatt tcaacttatg cagatgaaac tggtaacgta caggttgatc
     1501 cagctatcat tacctctatc caagaggctg accgtcttta tattttagcg gcagggactt
     1561 cctaccatgc tggttttgca acaaaaaata tgcttgagca attgacagat acaccagttg
     1621 agttgggagt ggcttctgag tggggttacc acatgcctct gcttagcaag aaaccaatgt
     1681 ttattctact aagccaatca ggagaaaccg cagatagtcg tcaagtttta gtaaaggcaa
     1741 atgctatggg cattccgagt ttgacagtaa ctaacgttcc aggatcaacc ttatcacgtg
     1801 aatcaacata caccatgttg attcatgctg gacctgaaat tgctgttgca tctacaaaag
     1861 cttacactgc acaaattgct gcccttgcct ttttggctaa ggcagttggt gaggcaaatg
     1921 gtaagcaaga agctcttgac tttaacttgg tacatgagtt gtcattggtt gcccaatcta
     1981 ttgaggcgac tttgtctgaa aaagatctcg tggcagaaaa ggttcaagct ttgctaacta
     2041 ctactcgtaa tgctttttac atcgggcgtg gcaatgatta ttacgttgcg atggaagctg
     2101 ctttgaaatt aaaagagatt tcttatattc aatgcgaagg ctttgcggct ggtgaattga
     2161 aacatggaac catttcatta attgaggagg acacgccagt aatcgcttta atatcgtcta
     2221 gtcagttggt tgcctctcat acgcgtggta atattcaaga agttgctgcc cgtggggctc
     2281 atgttttaac agttgtggaa gaagggcttg accgtgaggg agatgacatt attgtcaata
     2341 aggttcatcc tttcctagcc ccgattgcta tggtcattcc aactcaactg attgcttact
     2401 acgcttcatt acaacgtgga cttgatgttg ataagccacg taatttggct aaagctgtaa
     2461 cagtagaata aatgattata atggataagg caaccatctg ttaggtggtt gctttttgtt
     2521 tttaggagaa tagcagagct tagaaaatgg ccttgagaaa agctatacaa gaagaatagt
     2581 catcttctga tgagaacgta ttcatcttgt caaatccttg attctaaagt aaaatggtaa
     2641 cattaagaaa tgtttaggag tgtaagatgt cattaccaaa ttgtttgcaa tgccagtctg
     2701 aatatgttta cgaagatggt cttttattaa tctgtccaat gtgtgctttt gaatggacac
     2761 caggcgaaga agcgactgaa gaagaaggac ttgttgtttt agatagcaat ggtgttcgat
     2821 tatctgatgg cgatactatt actgtggtta aagatttgaa agtaaaaggc gctccaaaag
     2881 acctgaaaca agggactcgt gttaaaaata ttcgcctggt ggaaggtgat cataatattg
     2941 attgtaagat tgatgggttt ggcgcgatga aattgaaatc agaatttgtc aagaaaatct
     3001 aataataata cgcaaccagc tgatctggtt gtatttttat aaaaaaacct ctccccaaat
     3061 acgttcaaat ttggtataat agtaaaatat tcagttttag gaggcaacat gacctatatt
     3121 cagcaagttt tacccagctt attagatggt gccttggtga ccttacaagt attctttatt
     3181 gttatcattc tttctatacc cttaggggct attttagctt tcttgatgaa gattcccttt
     3241 aaaccgctac agtggttttt gaccctatac gtgtggatga tgcgagggac accattacta
     3301 cttcaattga tttttttcta ttatgttttg ccaagtgtgg ggattagttt tgatcgaatg
     3361 ccagctgcta ttttggcgtt tactttgaat tatgctgcct actttgctga aatttttaga
     3421 ggtgggattg aggctattcc aaaaggtcaa tatgaagcag ctaaagtatt aaagttaaaa
     3481 cctcttcaaa ccattcggta tattattttg cctcaagtgt ttaaaattgt gttaccaagt
     3541 gttttcaatg aagtcattaa tttggtcaaa gattcttccc ttgtctatgt actcggtgta
     3601 ggagatcttt tattagcaag taagacggca gccaataggg atgcaacctt agcccctatg
     3661 tttatcgctg gtcttatcta tttgctttta attggactgg tcacgattat ttcaaaacaa
     3721 gttgaaaaac ggtttaatta ttatcagtaa ggaggcttca tgttagaact caaaaatatt
     3781 tccaaacagt ttggtcaaaa aaccattttt gatggcttta atctaaccgt tcaagacgga
     3841 gaagtgctct ctttggtagg cccatcaggt ggcggtaaga caacactctt acggatgttg
     3901 gctggtcttg aatctattga ttcaggtcaa gtcttttaca atggcgaaga tgttggtatt
     3961 gatcatttgg aaaatcgtaa cttgcttgga tttgttttcc aagattttca attgtttcct
     4021 catcttaccg ttttagacaa cctaacgctg tctccaacaa ttacaatggg caagcaaaag
     4081 gctgacgcta aagaaaaggc tttggattta ctggcacgtt taggattaaa agaacatgcc
     4141 caagtttatc cctattcgtt atctggaggt caaaaacaac gggttgcttt ggcaagagcc
     4201 atgatgattg accctcaaat tatcggttac gatgaaccga ccagtgctct agatcctgaa
     4261 ttacgtcaga cggtagaagc cttgattgtt caaaaccgtg aaatgggaat aactcaaatt
     4321 gtagtcactc acgatcttgt ttttgccgaa gccatctcag atcgtattat tagggttaat
     4381 cctaaataga agaggaccca aaagatgatt ataaaaaaaa gaaccgtagc aattttagcc
     4441 atagctagta gctttttctt ggtaggttgt caagctacta aaagtcttaa atcaggagat
     4501 gcttggggag tttaccaaaa gcaaaaaagt attacagttg gttttgacaa tacgtttgtt
     4561 cctatgggct ataaggatga aagcggcaaa tgcaaaggtt ttgatattga tttggctaaa
     4621 gaagtttttc accaatatgg actcaaggtt aactttcaag ctattaattg ggacatgaaa
     4681 gaagcagaac taaacaatgg taaaattgat gtaatctgga atggttattc aataactaag
     4741 gagcgtcagg ataaggttgc ctttactgat tcttacatga gaaatgaaca aattattgtt
     4801 gtcaaaaaaa gatctgatat caaaacaata tcagatatga aacataaagt gttaggagca
     4861 caatcagctt catcaggtta tgactccttg ttaagaactc ctaaactgct gaaagatttt
     4921 attaaaaata aagacgctaa tcaatatgaa acctttacac aagcttttat tgatttaaaa
     4981 tcagatcgta tcgatggaat attgattgac aaagtatatg ccaattacta tttagcaaaa
     5041 gaagggcaat tggagaatta tcggatgatc ccaacgacct ttgaaaatga agcattttcg
     5101 gttggactta gaaaagaaga caaaacgttg caagcaaaaa ttaatcgtgc tttcagggtg
     5161 ctttatcaaa atggcaaatt tcaagctatt tctgagaaat ggtttggaga tgatgttgcc
     5221 actgccaata ttaaatctta aaaaggacac ttgtcctttt tttgtttaac aggtgagatc
     5281 tagcagacaa agtcagctag acgatttaat aaaaatctta gtttaaggtt ttttattaaa
     5341 ctgtattttt tctaaaaatt atagtttttt taaaactgat aaatgacaag agaagttatt
     5401 ttaggtcaat taacgtccat ttaaaagtaa tactaggcat ttaatgacat taaaaaatga
     5461 ttatttttgt gaaatcctct tattttctgg aaattaaaga aatattgacc ttagtaaaaa
     5521 caagtagtat gataacgtta taagcgctta taaattaatt taacttcact taactattta
     5581 aataggttta ttatattcag gaggaataat atgaaaccca aacaccttct ctgtctttca
     5641 acagttgttg ctggattagc tttattttca actatgacgt actcagtttt agctgatgat
     5701 gcttcaaatc ccgatactat tttaatgaat aacaaccaag cgaatcttca aagagatgct
     5761 ctagtgcaaa agcttgatga gggacatcaa caattagaag ctattaaaca tgaagctaaa
     5821 ggtactgata ttgaggctac tgttaacaaa gctatcgatg ctgttgatca catgaagagt
     5881 tctatacgct tcaatactga aacgatctat gattttagtt caattggggc aagagtagaa
     5941 gcattatcag atgctatcaa agcaatcgta ttttccacaa cccaattaac tcataaagta
     6001 gaaaaagcgc atactgatat ggggtttgct attactaaat tagtcattcg tattatagat
     6061 ccatttgcat ctgttgacgc tatcaaagct caagtacaag aaattaaagc acttgaggaa
     6121 aaagttatca attatcctga tttacagcca acagaccgtg ctactatcta tactaaagct
     6181 aaactaaata aagctatttg gaacactcgt ttagaaagag ataaaaaggt tttggggatc
     6241 aaaccatttg acgtttacaa tagacttaac aaagctatca cacatgcggt tggtgtccaa
     6301 ctaaatccga caaccactgt tcaacaagtt gacgatgaag ttattgctgt gcaaaatgct
     6361 ttggaaacag ctcttaagtc atagagtgta taagtaatgt gttgtatagg tagatgatta
     6421 cattaggata tagaataggt aaagatttta aatatcttac cattgtagag tttgaggtga
     6481 tgctctcata aacaatgagt cacctgtagc cctttttgcc tttaacatga tgtaccaatt
     6541 taaggtattg ttcaaccgtc actattgaat cttgtttaac ctcctaaact aataagattc
     6601 aatatgaaaa cgtgtattct taaaaaaatc cctatgatct gtttaaaaac aaattgtagg
     6661 gattttttta actatttaac ttatcatttt gggactagga atcgctttag gaatatcatg
     6721 aagtatagtt atgttataag agaattaatg tttttacatt tttacaatac gatagtgtgt
     6781 gtgatgtaat tgtctcttag cgataggggt aatttggtgg aagagtaagg accaagattc
     6841 atgacgcctc caaatactgg tacgttgatt gccatttaat tggtaagtta ccaacatcgt
     6901 tttttgacta atggattcaa tctgaaaatg gttgaagtta cttggataat aaatttcttg
     6961 attcaaaaat tgttcatagt taaatgtttg gccattcgca tcaatcagtt ggaaagactt
     7021 agcaatgtag tggtcatagt caggaacctt actcaaaatt aatttttcgt agcggtagag
     7081 ctttttggcg acattatcaa aaagctcttc ttcgggaatc atggcaggct caacgtgaat
     7141 gtcaatgtca taaatagaaa attggtcact caataactgc tccactttct ctgtaataga
     7201 gtgactttca tagactgaaa gatcaggatt catttcaagt acaatatcaa gatagacatt
     7261 gctaccatag gtcctagctc gttgagactt aacggcgaca attttaggga tttctaaaat
     7321 ggctttttcg tattttttca aatgacggct atcaaatcca tcagataaac taaacgaact
     7381 ttccataaag atatcaaaag ctgttttaag aataaagaac gtaatgatca tagcagctat
     7441 atgatcgata actggtaaat gcaaagaagc tgctataata gcaattgatg ttccgataga
     7501 agtaacagca tcagctagat tatccttaga agcagcgact aaggcactgg attttacacg
     7561 tttggaaaga cgtttgttaa agacatagac ccctaacata acaaaagctg aaacgatacc
     7621 gacaacagcc ccaagagggt caatatcaac ttgctgacca ctaaagatac ttttaattgt
     7681 gtgaatcagt acttggaaac ctacaagaaa cataataaaa gaagtgacaa ggctggataa
     7741 gtcttcaatt ttccaatgac caaatttatg attggcatcg gctggttggc tagctaagtg
     7801 aagaccaata agcagggcta catttcccac aatatccgat aaattgttaa atccatcagc
     7861 aataagggaa cttgcattta acaaataacc agctaataat ttggcaacac taagcgacaa
     7921 gtagacaata atactaacga ttgggccttt tctggctagt tttaaatttg caatcggatc
     7981 ctgtgtcata ataactcctt tgatttcaac tttatattat accataaaaa ggggcttaag
     8041 aaaaaagcct agcgtcttga acttggcaga agaagactag gtataactgg gtagtactaa
     8101 cgtcatgttt acttaataaa gtaatgcttg gtttctaaaa taacaactgg cgagagagct
     8161 aggagggcaa tcaggttagg aagtgccatt aaaccattga caatatcagc caaaacccaa
     8221 attagttcta acttaaggaa gcctcctagg cctaccatta agataaagac aatacggaag
     8281 taggtgagat gtgtaatgcc aaagaggaat tcaaagcagc gttctccata ataactccat
     8341 cctaaaatcg ttgtaaaggc gaagagaact agggaaaagg ttaggccaaa agtccctaaa
     8401 tttccaaaca cagtggcaaa ggcagattgg gtcagaggag ccccttcgag ctgcccagtc
     8461 cattggcctg ttactaaaat agataggccg gttagggtac agataataat cgtatcaata
     8521 aaagttcctg tcatggagat cagtccctgc tccacgggtt cattggtttt agctgctgct
     8581 gcagcaatag gtgcagatcc tagtccagac tcattggaga agacaccgcg tgcaattcct
     8641 ttttgaatgg catccttcat gagacttcca gcaaagcctc caatagcagc tgtcggagtg
     8701 aaggctgatt gaaagactaa acgaataaca ggaagtagct gctggtagtg actaaaaata
     8761 actgctaaac tagatagaat ataaaaaata gccataaaag gaacgacttt ttcagccact
     8821 ttggaaatgg aatgaatgcc accaaaaata atggcagcca caaagatagc taaaacaata
     8881 ctgaccattt gtggggataa gccaaagcta tgacctaaag acgaggtgat cgagttcact
     8941 tgagcaaagg ttcctattcc aaatagggca actaggatcc ctgaacccgc aaaaagaatg
     9001 gctaagggtt tccattttgt ccccatgcca ttaacaatat agtacatagg accaccagaa
     9061 atatgaccgt tggcatcctt tgtacggtat ttaatcgcta gtaccccttc agcgtactta
     9121 gtggccattc caaaaaaagc ggcaacccac atccaaaaga gcgctccagg accaccagac
     9181 ttgatagcag tggcaacccc aacaatgtta ccagttccga cagtagcggc aagggcagtt
     9241 gcaagagcag caaaggatga aatatctcca tgtccttcgt catctgaaaa aatgagttta
     9301 aaggctcttg gtagttttaa gatttgaatt aatcctaaac gactggtaag gtaaatcccc
     9361 gtcccaacca ataaaattaa gaggggcggt ccccaaacaa ggttatcaat taatttaacg
     9421 aatgctatca tctttctctc ctaaaagtca tcgaagacgc caaaagaaaa aagctatctc
     9481 tttctttgag acaacttctg aagcaatgat aaaacagcaa acacaaaaaa gactaagact
     9541 gttcttaatg gtgtttgtat tttaccttct gtccttttgc ctgagagatt gagcagtatg
     9601 ccttgcccct tcggcgccta ataaggtctc tccagaagtc cgtccatcaa acagtcccgt
     9661 tcaaaaactg aacacctgag agtataactc cttcggcgaa aaaaattctt ctcctgcatg
     9721 acatcattcg gtttattaaa ttaggactat catacctcaa aaaacgaatg aagtcaacca
     9781 aaatagcaaa aaaataagga atgctttaag aaatcgctat tatttttaaa tatctgatcc
     9841 taaactagaa aaaaggaatc tctttacttt tggggtcttc tttgctattt ttcttggaaa
     9901 catgatataa ttttaaggtt gataaaagag atgtcactga acgagattaa ggagaaagca
     9961 tgaatccttt attaaatgga atgaatgatc ggcaggcaca agctgttcaa acaactgaag
    10021 gccccttatt gattatggcg ggggctggtt ctggtaaaac aagggtactg acccatcgta
    10081 ttgcctattt aattgatgaa aaatttgtca atccttggaa tattttagct attaccttta
    10141 ctaataaggc tgcgcgtgaa atgaaagaaa gagccttgtc cttaaatcca gctactaaag
    10201 ataccttaat tgctaccttt cactccatgt gtgtacggat tctgcgtcgt gaggctgatc
    10261 acattggtta caaccgaaac ttcacaatcg tggatccagg tgagcaacga acgttgatga
    10321 agcgcatttt aaaacaatta aatatagacc ctaaaaagtg gaatgaacgt tctattttag
    10381 gaactatttc aaacgctaaa aatgatttgc ttgatgaaaa gggatacgag gcacaggctg
    10441 ctgatatgta tagtcaaatc gtagcgaggt gttacaaagc ttatcaggaa gaattacgac
    10501 gtagcgaagc acttgacttt gatgatttaa tcatgatgac gctacgcctg tttgacgcta
    10561 atcctgacgt gttagcttat taccagcagc gttaccaata cattcatgtg gatgagtacc
    10621 aagataccaa ccatgctcaa tatcagctaa ttaaattgct agcttctcgc tttaaaaata
    10681 tttgcgttgt tggagacgct gatcagtcta tctatggttg gcggggagct gacatgcaaa
    10741 atattcttga ttttgaaaaa gattacccag atgctaaggt agtgctttta gaagaaaact
    10801 atcgctctac caagaaaatt ctgcaagcag ctaacgacgt gattaacaat aaccgtaatc
    10861 gccgtcctaa gaaactttgg acacaaaatg cggatgggga acaactggtt tattacaggg
    10921 caaatgatga gcgagatgag gcagtatttg tggcctcaac cattagcaat atgagtcaag
    10981 agcttggaaa aaacttcaaa gatttcgctg ttttataccg taccaatgcc caatctcgga
    11041 ccattgaaga agcactctta aaatccaata ttccttacac catggttgga ggcaccaagt
    11101 tttacagccg taaagaaatt cgggatttga ttgcctattt aaccattgtt gctaaccctg
    11161 ctgataatat ttcttttgag cggattgtga atgagcctaa acgaggtgtt ggtccaggaa
    11221 cgcttgataa actgcgtcag tttgcctatg agagtgacca gtctttatta gaagcagctt
    11281 caaatctatt gatgtccccc ttgaaaggta aagcagcaca agctatcatg gacttggcca
    11341 atattctcgg tcagctacgt caagacctag atcagatgag tatcaccgac ttagcagaag
    11401 ctttgttaga aaaaacaggc tatttagata gcttacgcct gcaaaataca ttggaaagcc
    11461 aagcccgtat cgaaaatatc gaagaattct tatctgttac caaaaatttt gacgagtcaa
    11521 gtgcctctca agaagaagac gaaaccggtg ttgatcgctt gggacgtttt ttaaacgatt
    11581 tagcactgat tgctgataca gatgattccc aagcagaagc tgctgaagta accttaatga
    11641 ccctacatgc tgccaaaggc ttggagttcc ctgttgtttt cttaattggt atggaagaag
    11701 gtgtcttccc cttatcacgt gcctctgaag atccagatga attggaagaa gaaagacgtt
    11761 tagcttatgt cggaatcaca agagctgaag aagttctctt tatgaccaat gccaacacca
    11821 gaaccctctt tggtaaaagt agttataacc gtccaacacg cttcttgaaa gaaatttcag
    11881 aggaactact ctcttataaa ggattggcaa gaccagcaca atcttcattt ggtgtgcgtt
    11941 tttctactga aacacacaca cagtttggac aagggatgag cctatcagaa gccttacaag
    12001 ctcgaaaggc ccaggctcaa gtaagacaat cagcacagcc aatgcaggca catacgatac
    12061 catcagcttc aactagctca gttttaccgt ttggttctaa tagctctgta gaagaggtca
    12121 cttggcagat tggcgatatc gctcaccata aaaaatgggg agacgggact gtgcttgagg
    12181 tttcaggctc cggaaaaacg atggaattga aaatcaaatt tccagaagtt ggtttaaaga
    12241 aattactagc aagtgttgct cctattgaga aaaaataaga aaaaa
//
LOCUS       DAUCWO010000051        11891 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUCWO010000051 DAUCWO010000000
VERSION     DAUCWO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.051"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            142..1743
                     /locus_tag="VGY20_001318"
     CDS             142..1743
                     /locus_tag="VGY20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274168.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHRCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSP
                     SKSHSGKEVQKPEKPDNSVNQATSS"
     gene            1931..3190
                     /locus_tag="VGY20_001319"
     CDS             1931..3190
                     /locus_tag="VGY20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ4274169.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLATGQTIKADTS
                     EQEHPNITKIKEQLLRNKLVPATQWYQAYTKSEELSRQVEKLQTLVQSKTSNYEKIKS
                     QLQELEQSKKALEDTFYSTLRDHSSLFKEQDKEIKNMRAQLEFKNKQISDLTNENAQL
                     KEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEEIAKLQS
                     EAATLENLLGSAKRELTDLQAKLDTATAEKAKLESQVTTLENLLGSAKRELTDLQAKL
                     DAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAK
                     QAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEET
                     TNPFFTAAALTVIASAGILALKRKEES"
     gene            3415..4638
                     /locus_tag="VGY20_001320"
     CDS             3415..4638
                     /locus_tag="VGY20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ4274170.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFTNQTTVKADSS
                     SRDITEAGVSKFWKSKFDAEQNRANELEKKLSGYEKDYKTLEQEYENLEETLQKFGND
                     YERLENKNNEYANQLGKNQEEREKLELEYLRKSDKEYKEHQQYRQEQEERQKNLEELE
                     RQNKREIDKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKGLEAEHQKLKE
                     EKQISDASRKSLSRDLEASREAKKKVEADLAALNAEHQKLKEEKQISDASRKSLSRDL
                     EASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARLEAEAKALKE
                     QLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGEAANPFFTAAAATV
                     MVSAGMLALKRKEEN"
     gene            4658..5122
                     /locus_tag="VGY20_001321"
     CDS             4658..5122
                     /locus_tag="VGY20_001321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274171.1"
                     /translation="MINKFQRPSKKYYFLIALASLVLLGTSKTVFGESMTLYTGDYWY
                     EEDGGMLNGNHWGMENWWEPSSLWAPYPNPHYSENDPHQPPYGGSLLGLSSDNERERG
                     PRPTPSPQSPLTPESNPNNSRNQGPVNSPWPQGPLFGGLTSGTTSSWGSVIK"
     gene            5427..6551
                     /gene="enn"
                     /locus_tag="VGY20_001322"
     CDS             5427..6551
                     /gene="enn"
                     /locus_tag="VGY20_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Enn"
                     /protein_id="HEQ4274172.1"
                     /translation="MTRQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRADQA
                     ASEKVEVKESEKETKYKTLALRGENADLRNVNAKYLEKINAEEEKNKKLEATNKELNE
                     NYYKLQDGIDALEKEKEDLKTTLAKTTKENEISEASRKGLSRDLEASRAAKKELEAKH
                     QKLEAENKKLTEANQISEASRKGLSNDLEASRAAKKELEAKHQKLEADHQALEAKHQK
                     LAEEHQVSETSRKGLSRDLEASREANKKVTSELTQAKAQLLALEESKKLSEKEKAELQ
                     AKLDAQGKALKEQLAKQTEELAKLRAEKAAGSKTPATKPANKERSGRAAQTATRPSQN
                     KGMRSQLPSTGEAANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            6886..10434
                     /gene="scpA/B"
                     /locus_tag="VGY20_001323"
     CDS             6886..10434
                     /gene="scpA/B"
                     /locus_tag="VGY20_001323"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ4274173.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQAKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQNDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPEAKPE
                     QDGSGQTPDKEPETKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTRDQLP
                     TTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
     gene            10531..11799
                     /gene="fbpA"
                     /locus_tag="VGY20_001324"
     CDS             10531..11799
                     /gene="fbpA"
                     /locus_tag="VGY20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibronectin-binding protein FbpA"
                     /protein_id="HEQ4274174.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQQN
                     SSTQRNTANTSKKWYHIDDRGFIPVDISLETAKEDFKKEVKESRLSEEQQKTYNQKIE
                     AAKNKDDLLLTYHREYSEDIKKHPASTEPTTQPVEAPVQETQAPASDSMVTGDSTSVT
                     ADSPKETPSSESPVAPAPSIPEVPEAPASSPESEKSSVAASSEETSSPETPAAPEAPA
                     LSEAPAQPAESEKPSVAAPSEETPSPETPVAPETPEEPAAPAPSPESEEPSVAAPSEE
                     TPSPAAPALSEAPAQPAESEKPSVAASSEETPSPSTPAESETQTPPAVTKDSDKPSSA
                     AEKPAASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGE
                     KAQPLFIATMTLMSLFGSLLVTKRQKETKK"
BASE COUNT         4395 a         2391 c         2313 g         2792 t
ORIGIN      
        1 cgcatcattg accgctttac tcgttttaat gaaagaattt gaaagatatt ttatggtata
       61 cttttcctta atatggttca tacggacttg ataaatctat ttacaaaaat aataaaaaat
      121 taagataagg acatgaagtt aatgcatgta agtaaattgt ttactagcca acaatggaga
      181 gaattgaaac tgatctcata tttaacagaa aactccaatg ctatcggagt taaagataag
      241 gagcttagca aggctctcaa tatttccatg ttaacattac agtcatgcct cactaatatg
      301 cagtttatga aagaagtagg tggaattacc tacaaagacg gctatattaa catttggtat
      361 caccggtgtt gtgggctaca agaggtctat caaaaagctc ttcgagaatc gccttctttg
      421 aaattactgg agcttttgtt tttcagagac tttagttcct tagaagagtt agctgaagag
      481 ctgtttgtca gtttatctac cctcaaacgc ctcatcaaaa aaaccaacac ttacttgtct
      541 catacctttg ctattagtat cgtgacaagt ccagtgcagg tatcaggaga tgagaggcaa
      601 atccgcctgt tttacctcaa gtatttttca gaagcctata aaatctcaga atggccattt
      661 ggggacattt taaacctgaa aaactgtgaa cgcctattga gcttattgat caaagaagta
      721 gatgtcaaag ttcattttac actatttcag cacttaaaaa tcttaagtgg tgtcaacttg
      781 atccgttact acaaggggta ttcttgcagt tataacaata aaaaaaccag tcaccgtttt
      841 tctcaactta tccaacattc ctcagaaatc caggatcttt ctcgcttgtt ctacctcaaa
      901 tttgggttac atctcgacga gtataccata gctgaaatgt tttctaacca tcttaacgac
      961 aagctagaaa ttggctgtgc ttttgagatt atcaatcaag acccaacatc aggaggcaga
     1021 caagtaacca actggatcca tctattagat gagatggaaa tcaagctcaa tctcagcatc
     1081 accaataaat acgaagttgc tgtaaccctc cataacgctt cggtgttaaa cgaagaagac
     1141 attacagcca attatttgct gtttgattat aaaaagagtt acctgaactt ttaccaaaaa
     1201 gaacaccctc gtatctatga ggcctttgtg acaagtgtcg agaagctgat gcaggcagat
     1261 aatgctcaag ttagcaaaga attgatcaat cagctcactt attgtttctt tattacctgg
     1321 gaaaatagtt tcttaaaagt aaatcaaaaa gatgaaaaag tacgacttct agtgatagag
     1381 agaagttaca atagtgtcgg taattttttg aaaaagtaca tcggtgagtt tttcagcatt
     1441 actaattttg atgagttaga ttgtttgaca attgatctag tagagattga aaaacagtac
     1501 gatgttatcg tgacagatgt tatggtgggt aaaagcgaag agttagagat tttctttttc
     1561 tacaagatga tcccagaagc tattattgat agattaaacg aatttttgaa cgttagtttc
     1621 acagataaca acgttatggt caaaaacctc gaagcccctt ccccttcaaa atctcatagt
     1681 ggcaaagagg tacaaaagcc agaaaagcca gacaattcag ttaatcaagc aacatcatca
     1741 taggatttca gacgtcatgg taagaaaaaa aagaatcatt tacagcccat agaggtaagg
     1801 tcaaaagctg aaagcagctc aaaaaaactg acctttacct tttggctttt tttatttaga
     1861 ataattttat tggagagatg cttaataatt taagcacaat tcttagaaat tgagaaataa
     1921 ggagtaaaca atgtctaaaa gaaatccaaa caaacactac tcactgagaa agttaaaaac
     1981 aggtactgca tcagtagcag tagctttgac agttttgggg actgggttag caactggaca
     2041 aacaataaaa gctgatacca gtgaacaaga acacccgaac attacaaaga tcaaagaaca
     2101 gcttttacgc aataagttgg tccctgctac acaatggtac caagcctata ctaaaagcga
     2161 ggaacttagt cgtcaggtcg aaaaacttca gaccttggtc caatcaaaga cctcaaatta
     2221 tgaaaagata aagtcacagt tacaagagtt ggagcaatct aaaaaggctt tggaggacac
     2281 tttttacagt acacttagag atcactcaag tcttttcaaa gaacaagata aagaaatcaa
     2341 aaatatgagg gcccagcttg agtttaaaaa caagcaaatc tcagatttaa ccaacgaaaa
     2401 cgcacagtta aaagaagcca ttgaaggtta tgtgcaaact atccaaaacg ctagtcgtga
     2461 aatcgcagca aaacaacaag aacttgcagc tgcaaaaagc cagttagagg caaaaaatgc
     2521 tgagattgag gcattgaaac aacaagatgc ctctaagact gaagaaattg ctaaattgca
     2581 atcagaagca gcaactttag aaaaccttct aggttcagcc aagcgtgagt tgactgactt
     2641 gcaagctaag ctagatacag caactgctga aaaagcaaaa ctagaatcac aagtaacaac
     2701 cttagaaaac cttctaggtt cagctaagcg tgaattgact gatctgcaag ctaagctaga
     2761 tgcagctaac gctgaaaaag aaaagctcca atcacaagca gcagccctag aaaaacaact
     2821 agaagcaact aaaaaagagt tagctgattt acaggctaaa ttagcagcaa ccaaccaaga
     2881 aaaagaaaag ttagaagctg aagcaaaagc tcttaaagag caattagcaa aacaagctga
     2941 agagcttgct aagctaaaag cagataaagc ttcaggggct caaaaaccag atactaaacc
     3001 tggcaataaa gaggttccaa caagaccatc acaaacaaga acaaacacta ataaagctcc
     3061 tatggcgcaa acaaagagac aattaccgtc aacaggcgaa gaaacaacca acccattctt
     3121 cactgcagca gcattgacag tgatcgcatc tgcaggcata cttgccctaa aacgcaaaga
     3181 agaaagctaa gcccaactca caatatcttt tctagcccaa gaaaaaaaca aaaaaagagg
     3241 aagaccattc ctcttttttt gaacggttga aaagcaaaaa ggtcaaaaag gcactaaagt
     3301 ctcaaaaacc tggtctttac cttttaccgc ttattcttta gaatagaatt attagagaga
     3361 agtcttagaa aaatgaggct aattccctaa agatgaaaaa ataaggagca aataatggct
     3421 agaaaagata cgaataaaca gtattcgctt agaaaattaa aaacaggtac agcatcagta
     3481 gcggtcgctg tggctgtttt aggagcaggc tttacaaacc aaacaacagt taaggcggac
     3541 tcttctagta gagatatcac tgaggcaggt gtatctaagt tttggaagag taagtttgat
     3601 gcagagcaga atagagcaaa cgagcttgaa aaaaaactta gtggatacga aaaagattat
     3661 aaaacattag aacaagagta tgaaaatcta gaagaaactt tacaaaaatt cggaaatgat
     3721 tatgaaagat tagaaaacaa aaataatgag tacgcaaatc aacttggtaa aaatcaagaa
     3781 gaacgcgaaa aattagagct tgaatacctc agaaaatcag ataaagagta taaagagcat
     3841 caacaatatc gacaagaaca agaagaacgt caaaaaaatc tagaggaact tgaacgtcaa
     3901 aataaacgag aaatagacaa acgctatcaa gaacaactcc aaaaacaaca acaattagaa
     3961 acagaaaagc aaatctcaga agctagtcgt aagagcctaa gccgtgacct cgaagcttct
     4021 cgtgcagcta aaaaaggcct tgaagctgag caccaaaaac tcaaagagga aaaacaaatc
     4081 tcagacgcaa gccgtaagag cctaagccgt gaccttgaag cgtctcgcga agctaagaaa
     4141 aaagtagaag cagacctagc tgctcttaat gctgagcacc aaaaactcaa agaggaaaaa
     4201 caaatctcag acgcaagccg taagagccta agccgtgacc ttgaagcgtc tcgcgaagct
     4261 aagaaaaaag tagaagcaga cttagccgaa gcaaatagca aacttcaagc ccttgaaaaa
     4321 ctaaacaaag agcttgaaga aggtaagaaa ttatcagaaa aagaaaaagc tgagttacaa
     4381 gcaagactag aagcagaagc aaaagctctt aaagagcaat tggctaaaca agctgaagaa
     4441 cttgccaaac taaaaggcaa ccaaacacca aacgctaaag tagccccaca agctaaccgt
     4501 tcaagatcag caatgacgca acaaaagaga acgttaccgt caacaggcga agcagctaac
     4561 ccattcttta cagcagcagc tgcaacagtg atggtatctg caggtatgct tgccctaaaa
     4621 cgcaaagaag aaaactaaaa tataaaaggg atcaatgatg attaacaaat ttcaacgtcc
     4681 gtctaaaaaa tattattttc ttatcgcttt ggcaagttta gtgctgttgg gaacttctaa
     4741 gaccgtcttt ggagaatcaa tgacactgta tacaggagat tattggtacg aggaagatgg
     4801 tggtatgtta aacggaaacc actggggaat ggaaaattgg tgggagccat caagtttgtg
     4861 ggctccttat ccaaatcctc actatagtga aaacgatcca caccagccac cttatggcgg
     4921 atccttattg ggattatcct cagataatga gagagaacgt gggcctagac ctactccatc
     4981 gccacagtct cctttaactc cggagtcaaa tcctaacaac tctagaaatc agggtcctgt
     5041 taatagccca tggcctcaag gtccgctttt tggaggatta acaagtggaa ctacctccag
     5101 ttggggaagt gttattaaat agtttgttat aagttatcca taatttctaa atcaaacaac
     5161 cgccccattc tttacagtag caggtatgct tgctctaaaa cgtaaagaag aaaactaagc
     5221 tattagactg atgctaaagc taagagagaa tcaaatgatt ctctcttttt gagtagctaa
     5281 gtaactaaca atctcagtta gaccaaaaaa tgagaatggc tcaaaaagct ggcctttact
     5341 ccttttgatt aaccatatat aataaaaacg ttaggaaaat aatagcacta ttaagttgct
     5401 ttttcaataa aatcaaggag tagataatga ctagacaaca aaccaagaaa aattattcac
     5461 tacggaaact aaaaaccggt acggcttcag tagccgttgc tttgaccgtt ttgggcgcag
     5521 gttttgcaaa ccaaacggaa gtaagagctg atcaagcagc ttctgaaaaa gtggaagtaa
     5581 aagaaagtga aaaagagacc aagtataaga cgttggcctt aagaggtgaa aatgctgacc
     5641 ttagaaatgt aaatgcaaaa tatttagaga aaattaacgc agaagaagaa aaaaataaaa
     5701 agcttgaagc aactaataaa gagctaaatg agaattatta caaattacaa gatggcattg
     5761 atgctttaga aaaagaaaaa gaagatctca aaacaacttt agctaagact actaaagaaa
     5821 atgagatttc agaagctagc cgtaaagggt taagccgaga cttagaagct tctcgtgcag
     5881 ctaaaaaaga gctagaagct aagcatcaaa aattagaagc agaaaacaaa aaactaacag
     5941 aagccaatca gatttcagaa gctagtcgta aaggtctaag taacgactta gaagcttctc
     6001 gtgcagctaa aaaagaacta gaagctaagc accaaaaatt agaggctgat caccaagccc
     6061 tagaagctaa gcaccaaaaa ttagcagaag aacatcaagt ttcagagact agccgcaagg
     6121 gtctaagccg tgaccttgaa gcatcacgtg aagctaataa gaaggttaca tctgagttaa
     6181 cacaagcaaa agctcaactc ttagcgcttg aagaaagtaa gaaattatca gaaaaagaaa
     6241 aagctgagtt acaagcaaaa ctagatgcac aaggaaaagc cctcaaagaa caattagcaa
     6301 aacaaactga agagcttgca aaactaagag ctgaaaaagc agcaggttca aaaacacctg
     6361 ctaccaaacc agctaataaa gaaagatcag gtagagctgc tcaaacagct acaagaccta
     6421 gccaaaataa aggaatgaga tcacaattac cgtcaacagg cgaagcagct aacccattct
     6481 ttacagcagc agctgcaaca gtgatggtat ctgcaggtat gcttgctcta aaacgcaaag
     6541 aagaaaacta agcctttaaa acttggtttt tgtaacgatg caatagacaa aagcaagcaa
     6601 ggccaaaaac tgagaaagtc ctaaaaagct ggcctttacc cctcaaaatt aatgttttat
     6661 aataaagatg ttagtaatat aattgataaa tgagatacat ttaatcatta tggcaaaagc
     6721 aagaaaaata gctgtatcat atgcaaataa cccctgtttg ctctttaaaa aagacgttat
     6781 ccttatttct ccacgcacag atggacagct aggagagaat cgtttgattc tctcttttct
     6841 taatggtcat aaagacaaag tctctcatca tgaaaggacg acacattgcg taaaaaacaa
     6901 aaattaccat ttgataaact tgccattgcg ctcatgtcta cgagcatctt gctcaatgca
     6961 caatcagaca ttaaagcaaa tactgtgaca gaagacactc ctgctaccga acaagccgta
     7021 gaaactccac aaccaacagc ggtttctgag gaagcaccat catcatcaaa ggaaactaac
     7081 cctccacaaa ttcctgatga cgcagaagaa acagtagcag ataaggctaa tgatccagcc
     7141 cctcaagctc ctgctaaaac tgctgataca ccagcaacct caaaagcgac tattagggat
     7201 ttgaacgacc cttctcaggt caaaaccctg caggaaaaag caggcaaggg agctgggact
     7261 gttgttgcag tgattgatgc tggttttgat aaaaatcatg aagcgtggcg tttaacagac
     7321 aaaaccaaag cacgttacca atcaaaagaa gatcttgaaa aagctaaaaa agagcacggt
     7381 attacctacg gcgagtgggt caatgataag gttgcttatt accacgacta tagcaaagat
     7441 ggtaaaaccg ctgtcgatca agagcacggc acacacgtgt cagggatctt gtcagggaat
     7501 gctccatctg aaacgaaaga accttaccgc ctagaaggtg cgatgcctga ggctcaattg
     7561 cttttgatgc gtgtcgaaat tgtaaatgga ctagcagact atgctcgtaa ctacgctcaa
     7621 gctatcagag atgctgtcaa cttgggagct aaggtgatta atatgagctt tggtaatgct
     7681 gcactagctt acgccaacct tccagacgaa accaaaaaag cctttgacta tgccaaatca
     7741 aaaggtgtta gcattgtgac ctcagctggt aatgatagta gctttggggg caagacccgt
     7801 ctacctctag cagatcatcc tgattatggg gtggttggga cacctgcagc ggcagactca
     7861 acattgacag ttgcttctta cagcccagat aaacagctca ctgaaactgc tacggtcaaa
     7921 acagacgatc atcaagctaa agaaatgcct gttctttcaa caaaccgttt tgagccaaac
     7981 aaggcttacg actatgctta tgctaatcgt gggacgaaag aggatgattt taaggatgtc
     8041 aaaggcaaaa ttgcccttat tgaacgtggc gatattgatt tcaaagataa gattgcaaac
     8101 gctaaaaaag ctggtgctgt aggggtcttg atctatgaca atcaagacaa gggcttcccg
     8161 attgaattgc caaatgttga ccagatgcct gcggccttta tcagtcgaaa agatggtctc
     8221 ttattaaaag acaatcccca aaaaaccatc accttcaatg cgacacctaa ggtattgcca
     8281 acagcaagtg gcaccaaact aagccgcttc tcgagctggg gtctgacagc tgacggcaat
     8341 attaagccag atattgcagc acccggccaa gatattttgt catcagtggc taacaacaag
     8401 tatgccaaac tttctggaac tagtatgtct gcgccattag tagcgggtat catgggactg
     8461 ttgcaaaagc aatatgagac acagtatcct gatatgacac catcagagcg tcttgattta
     8521 gctaaaaaag tattgatgag ctcagcaact gccttatatg atgaagatga aaaagcttat
     8581 ttttctcctc gccaacaagg agcaggagca gtcgatgcta aaaaagcttc agcagcaacg
     8641 atgtatgtga cagataagga caatacctca agcaaggttc acctgaacaa tgtttctgat
     8701 aaatttgaag taacagtaac agttcacaac aaatctgata aacctcaaga gttgtattac
     8761 caagcaactg ttcaaacaga taaagtagat ggaaaacact ttgccttggc tcctaaagca
     8821 ttgtatgaga catcatggca aaaaatcaca attccagcca atagcagcaa acaagtcacc
     8881 gttccaatcg atgctagtcg atttagcaag gacttgcttg cccaaatgaa aaatggctat
     8941 ttcttagaag gttttgttcg tttcaaacaa gatcctaaaa aagaagagct tatgagcatt
     9001 ccatatattg gtttccgagg tgattttggc aatctgtcag ccttagaaaa accaatctat
     9061 gatagcaaag acggtagcag ctactatcat gaagcaaata gtgatgccaa agaccaatta
     9121 gatggtgatg gattacagtt ttacgctctg aaaaacaact ttacagcact tactacagag
     9181 tctaacccat ggacgattat taaagctgtc aaagaaggag ttgaaaacat agaggatatc
     9241 gaatcttcag agatcacaga aaccattttt gcaggtactt ttgcaaaaca aaacgatgat
     9301 agccactact atatccaccg tcacgctaat ggcaaaccat atgctgcgat ctctccaaat
     9361 ggggacggta acagagatta tgtccaattc caaggtactt tcttgcgtaa tgctaaaaac
     9421 cttgtggctg aagtcttgga caaagaagga aatgttgttt ggacaagtga ggtaaccgag
     9481 caagttgtta aaaactacaa caatgacttg gcaagcacac ttggttcaac ccgttttgaa
     9541 aaaacgcgtt gggacggtaa agataaagac ggcaaagttg ttgctaacgg aacctacacc
     9601 tatcgtgttc gctacactcc gattagctca ggtgcaaaag aacaacatac tgattttgat
     9661 gtgattgtag acaatacgac acctgaagtc gcaacatcgg caacattctc aacagaagat
     9721 cgtcgtttga cacttgcatc taaaccaaaa accagccaac cggtttaccg tgagcgtatt
     9781 gcttacactt atatggatga ggatctgcca acaacagagt atatttctcc aaatgaagat
     9841 ggtaccttta ctcttcctga agaggctgaa acaatggaag gcgctactgt tccattgaaa
     9901 atgtcagact ttacttatgt tgttgaagat atggctggta acatcactta tacaccagtg
     9961 actaagctat tggagggcca ctctaataag ccagaacaag acggttcaga tcaagcacca
    10021 gacaaaaaac cagaaactaa accagaacaa gacggttcag atcaagcacc agacaaaaaa
    10081 ccagaaacta aaccagaaca agacggttca ggtcaagcac cagacaaaaa accagaagct
    10141 aaaccagaac aagacggttc aggtcaaaca ccagataaag aaccagaaac taaaccagaa
    10201 aaagatagtt caggtcaaac accaggtaaa actcctcaaa aaggtcaacc ttctcgtact
    10261 ctagagaaac gatcttctaa gcgtgcttta gctacaaaag catcaacaag agatcagtta
    10321 ccaacgacta atgacaagga tacaaatcgt ttacatctcc ttaagttagt tatgaccact
    10381 ttcttcttgg gattagtagc tcatatcttt aaaacaaaac gcactgaaga ttagtacaac
    10441 tgatcagatg gctactattg atgatgagtt agatcagtct tcttttcaaa agaaggcata
    10501 gcagtataaa aatagatatg tgaggacaat atgcgtagag cagaaaataa caaacacagc
    10561 cgctattcca ttcgcaaact gagcgttggg gtaacgagta tagcaattgc gagtctcttt
    10621 ttaggaaagg ttgcctatgc cgtagatagc atgcagcaaa actcttcgac tcaaagaaac
    10681 acagccaata catccaaaaa atggtatcat attgatgata ggggctttat tcctgtagat
    10741 ataagcttag aaactgccaa agaggatttt aaaaaagaag taaaagaatc acgtttgtct
    10801 gaagaacaac agaaaacgta taatcaaaaa attgaagctg caaaaaacaa agacgaccta
    10861 ttgttaacat atcatagaga gtactctgaa gatattaaga aacacccagc gtctactgag
    10921 cctactactc agccagttga ggcacccgtg caggagacac aggccccagc ttcagattcg
    10981 atggtgacag gtgattcaac atcagttacg gctgattctc ctaaggaaac cccatcttcg
    11041 gaaagtccag tagccccagc tccatcaatt ccagaggtcc cagaagcccc agcttcatca
    11101 cctgagagtg agaaatcttc agtagcagct tcttctgagg aaacctcatc tccagaaact
    11161 ccagcggccc cagaagcccc agctttatct gaggctccag ctcaaccagc tgagagtgag
    11221 aaaccttcag tagcagctcc ttctgaggaa accccatctc cagaaactcc agtggcccca
    11281 gaaactcctg aagagccagc agctccagct ccatcacctg agagtgagga accttcagta
    11341 gcagctcctt ctgaggaaac cccatctcca gcggccccag ctttatctga ggctccagct
    11401 caaccagctg agagtgagaa accttcagta gcagcttctt ctgaggaaac cccatctcca
    11461 tcaactccag ctgaatcaga gactcagacg ccaccagctg ttactaaaga ctctgataag
    11521 ccatcttcag cagctgaaaa accagcagcc tcttcacttg tttcagaaca aaccgttcaa
    11581 caaccaactt caaagagatc ttctgataaa aaagaagagc aagaacagtc ttactctcca
    11641 aatcgctcat tgtcaagaca ggttagggcc catgagtcag gtaagtactt gccttcaaca
    11701 ggtgaaaaag cgcagccact ctttatagct actatgactt tgatgtctct atttggaagt
    11761 cttttagtca caaaacgcca aaaagaaact aaaaaatagt attacttaaa ctgataagag
    11821 cttatttaat agagagaacc aatttggttc tctctatttt tttagatgtg ctgcctcatc
    11881 aaaaagaagt t
//
LOCUS       DAUCWO010000052        11804 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUCWO010000052 DAUCWO010000000
VERSION     DAUCWO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.052"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..225)
                     /locus_tag="VGY20_001325"
     CDS             complement(<1..225)
                     /locus_tag="VGY20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ4274175.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILN"
     gene            546..1829
                     /locus_tag="VGY20_001326"
     CDS             546..1829
                     /locus_tag="VGY20_001326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ4274176.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            2041..2616
                     /locus_tag="VGY20_001327"
     CDS             2041..2616
                     /locus_tag="VGY20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ4274177.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            2873..4477
                     /locus_tag="VGY20_001328"
     CDS             2873..4477
                     /locus_tag="VGY20_001328"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ4274178.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            4645..5571
                     /locus_tag="VGY20_001329"
     CDS             4645..5571
                     /locus_tag="VGY20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4274179.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            5625..5710
                     /locus_tag="VGY20_001330"
     tRNA            5625..5710
                     /locus_tag="VGY20_001330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5659..5661,aa:Leu,seq:aag)
     gene            5916..6797
                     /locus_tag="VGY20_001331"
     CDS             5916..6797
                     /locus_tag="VGY20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ4274180.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            7179..7367
                     /locus_tag="VGY20_001332"
     CDS             7179..7367
                     /locus_tag="VGY20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ4274181.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            7519..7884
                     /locus_tag="VGY20_001333"
     CDS             7519..7884
                     /locus_tag="VGY20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4274182.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            7884..9548
                     /locus_tag="VGY20_001334"
     CDS             7884..9548
                     /locus_tag="VGY20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ4274183.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            9785..10675
                     /locus_tag="VGY20_001335"
     CDS             9785..10675
                     /locus_tag="VGY20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ4274184.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            10905..11759
                     /locus_tag="VGY20_001336"
     CDS             10905..11759
                     /locus_tag="VGY20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ4274185.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
BASE COUNT         3941 a         1918 c         2522 g         3423 t
ORIGIN      
        1 gtttaaaatg ttgtgagtga gcttagagag tgtttttttg cgagcttcac tttgtcttga
       61 ataagcaaac gatttgttaa tggttttttc aaaaagatag ttagtaacac gttttaaaat
      121 agcaaaaaag agtaatagaa taatgagaga cactagcttt ttaaaaatcg ttaagccaat
      181 attttcgata tgagactgct ctaggtaatg taatataaat gtcatgagaa atcctttctt
      241 tgatagttta tcaaaaaaaa accaatcttg aaaagagata aattaatatt ttaaccaaag
      301 ttatgataat tgttttatca taaagaatgc catagtcatc cgttgtgata atgcttgaaa
      361 atagtcagtt agtaagcgaa aaagtctaac attaaccttt ataaattgaa aataactgtt
      421 agtagactaa tgacaggtag atactcttaa tagcgcgttt gatttcacgc ataaaaagct
      481 ttaaaaacgc tttaaattat gataaaatga actgtatcga aattatttta aggagaaatg
      541 acttaatgtc tacatcattt gaaaacaaag ctacaaatcg tggcgtgatt acatttacaa
      601 tcagtcaaga taaaataaaa ccagctcttg ataaagcttt taataaaatc aaaaaagact
      661 taaatgcacc aggtttccgt aaaggacaca tgcctcgtcc agtcttcaac caaaaatttg
      721 gtgaagaagt tctttatgaa gatgctttga atattgtatt gccagaagct tatgaagcag
      781 ctgtgacgga acttggtctt gatgtggttg cacaaccaaa aatcgacgtt gtgtcaatgg
      841 aaaaagggaa agagtggaca ctttctgctg aagttgtgac aaaacctgaa gtgaaacttg
      901 gtgattacaa aaaccttgtt gtagaagttg atgcttcaaa agaagtttca gatgaagacg
      961 tggatgctaa aattgagcgc gaacgtcaaa accttgcgga actcattatt aaagacggtg
     1021 aagcagctca aggtgacact gttgtgattg actttgttgg ttcagttgat ggtgttgagt
     1081 ttgatggcgg taaaggagat aacttctctc ttgaacttgg ttcaggacaa tttatcccag
     1141 gttttgaaga tcaactagtt ggtgctaaag ctggcgatga agtagaagtt aatgtcacat
     1201 tcccagaatc ttaccaagca gaagatcttg caggtaaagc cgctaaattt atgacaacta
     1261 ttcacgaagt caaaacaaaa gaagtacccg agcttgatga tgagcttgca aaagatattg
     1321 atgaagatgt tgacacactt gaagacttaa aagtaaaata tcgtaaagaa cttgaagcag
     1381 ctcaagaaac tgcttatgat gatgctgttg aaggagctgc gattgaatta gcagttgcaa
     1441 atgctgaaat tgttgattta cccgaagaaa tgattcatga agaagtcaac cgttcagtga
     1501 atgaatttat gggcaacatg caacgtcaag gaatctcacc tgaaatgtac ttccaattga
     1561 ctggtacaac tcaagaagat ttacataacc aatattcagc tgaagctgac aaacgtgtta
     1621 aaacaaacct tgttattgaa gcaattgcta aagcagaagg ttttgaagca acagatagtg
     1681 aaattgaaca agaaattaat gaccttgcaa cagaatataa catgccagct gaccaagttc
     1741 gttctcttct ttcagcagat atgttgaaac atgatattgc aatgaaaaaa gcagttgaag
     1801 tgattacaag cacagcaagc gttaagtaat cttattaagg aacaaaacgt acaagccgga
     1861 ttagaacgtc tttctagtct ggtttttctt tttctaaaga taaggaatta gttaaacgtt
     1921 attttaatga attagcttta aagatttgtc aagaagaagt gatatgagtt gataaaagtt
     1981 ttgacgaatt acttttttta gcgtaaaatg gtagagaatg ataaaataag gagaactgcc
     2041 ttgaaattag acgtatttgc aggacaagaa aaaagcgagc tttccatgat tgaagtggcg
     2101 cgtgccattt tggaagaacg cggtcgcgac aatgagatgt actttagcga tttagtcaat
     2161 gagattcaaa attacttagg aaaatctgat gcaggtattc gtcatgcatt accatttttc
     2221 tatactgact taaatacgga tggctcattt attcctctag gtgaaaacaa atggggactt
     2281 cgttcgtggt atgctatcga tgaaattgat gaagaaatta ttactctaga agaagatgaa
     2341 gatggcgcac aaaaacgtaa gaaaaaacgc gttaatgctt tcatggatgg agatgaagac
     2401 gctattgatt atagagatga tgatccagaa gatgaagatt tcacagaaga atcagctgag
     2461 gttgaatatg atgaagaaga tccagatgat gaaaaatcag aagtggagtc atatgattca
     2521 gaattgaatg aaattatccc tgaagatgat tttgaagaag ttgatatcaa tgaagaagac
     2581 gaagaagatg aagaagatga agaaccagtt ctctaatgtc tttagttcag aaataaaaat
     2641 gtaattatca tttgacaaat tgtctattat agtttatatt attattcggg cacctcttta
     2701 agaggtcagg caaaagctct ctagttaata gggggctatt tttgttttct agaataatgc
     2761 cagtttgcct ctatatttag aaaactaagg tgatacctaa ccaatgtgga accaactaat
     2821 agggaattaa caagaaagca atgcttttat tcagcttata taaaggagta ctatgacgaa
     2881 atacattttt gtaactggtg gtgttgtatc atcaattggc aaaggaattg ttgcggcaag
     2941 tcttggccga ttgttaaaaa atcgtggact taaggtgacg attcaaaaat ttgatcctta
     3001 tattaatatt gatccaggga cgatgagccc ctatcagcat ggagaagtat atgtgacaga
     3061 tgacggtgct gaaaccgact tggatttggg tcactatgaa cgttttattg atatcaatct
     3121 taataaatat tccaatgtta caacaggtaa aatttatagt gaagtccttc gtaaagaacg
     3181 taaaggtgag taccttggag cgactgttca ggtgattcct catatcacag atgcccttaa
     3241 agaaaaaatc aaacgcgcag cttcaacaac ggactctgat gtaattatta ctgaggtcgg
     3301 ggggactgtt ggcgatattg aaagtttacc gttcttagaa gctcttcgtc agatgaaagc
     3361 tgatgttggt tctgaaaatg tgatgtatat tcacaccacc ttgttgccat atttaaaggc
     3421 agcgggtgaa atgaaaacta agccaacgca acattccgtt aaagaattgc gtggtttagg
     3481 gattcaaccg aatatgttgg ttattcgtac ggaagagcca gttgagcagg ggattaaaaa
     3541 taaattggct cagttctgtg atgtgaattc agaagctgtt atcgagtcac gtgatgtaga
     3601 acacttgtat caaattccat tgaacttgca agctcaatca atggatcaga ttgtttgtga
     3661 tcatttgaaa ttgaatgcgc cacaagctga tatgaccgag tggtcagcta tggttgacaa
     3721 ggtgatgaat ttaagaaaaa caacaaaaat tgccttggtt ggtaaatatg tggaattgcc
     3781 agatgcctac ttatctgttg tggaagcctt gaaacattct ggttatgcca atgataccgc
     3841 aattgaccta aaatgggtca atgctaacga tgtgacggtg gataatgcag ctgacttgtt
     3901 gggagatgcg gatgggatta ttgtaccagg tggttttggt caacgtggta cagaaggcaa
     3961 gatccaagcg attcgctatg cccgcgaaaa tgatgtgcca atgcttggta tctgtcttgg
     4021 catgcagttg acttgtgttg aatttgcccg ccatgtcctc aatatggaag gtgcaaactc
     4081 ttttgaatta gagccaagta ctaaataccc catcattgat atcatgcgtg atcaaattga
     4141 tattgaagat atgggaggta cgcttcgtct gggattgtac ccatgtaaat tgaaaccagg
     4201 ttcaaaagca gcaatggctt acaataatca agaggtggtg caacgtcgtc atcgtcaccg
     4261 ttatgagttc aacaacaaat tccgtccaga atttgaagca gctggttttg ttttctcagg
     4321 ggtatcacct gacaatcgct tagttgaaat tgtagaattg aaagagaaaa aattctttgt
     4381 agcagctcaa taccacccag aattgcaaag ccgtccaaat cgtcctgagg agctatacac
     4441 cgcttttgtt acagctgcta ttaaaaatag caattagtgg ttttaagggt aagtttgtgg
     4501 cattctactt ggctggcaga tactgataaa gattactagc tattagaggg atcttttcta
     4561 taaaaatagc attagaaaaa gaaaaacgtg atatttttag gttagcactt tctttttcag
     4621 tcattttttt gtaaaatact tggtatgaaa actattcgaa tagcaaagta tttaggaatt
     4681 ctattccttt tgataactct tattagcgta ggtgctagtt tttatttctt tcatgttgca
     4741 caaataagag aagagaaatc gtttattaat aataagaaac ggagtacgaa taatccatta
     4801 tacccagctg aacagtcttt tgacgcttta ccttacgaaa aacgtcaact aacaaatcgt
     4861 gggttaaaac aagtggggtg gtacttacca gctgctcaaa aaacaaaaaa gacagctatt
     4921 gttgttcatg gttttacgaa tgacaaagaa gatatgaagc catatgccat gctttttcat
     4981 gatttgggct ataatgtctt aatgccagac aatgaggccc atggggaaag tgaagggaac
     5041 ttgattggtt atggctggaa tgaccgcctt aatgtcatgg cttggacaga ccaactgatt
     5101 aaggaaaacc ctgaaagcca aatcacactc tttggcttat ctatgggtgc tgcaacagta
     5161 atgatggcaa gtggtgagcg attgcctgcg caagtcacct ccctcatcga agattgcggt
     5221 tataccagtg tttgggacga attgaagttt caggccaagg ctatgtacaa cttgcctgcc
     5281 tttcctttac tctatgaagt ctctgcctta tctaagattc gagcaggttt tagttacgga
     5341 gaagcgagct cagtgaaaca gctggctaaa aataaacgtc caactttatt tatccacggt
     5401 gataaggatg attttgttcc tacaaaaatg gtttatgaca attataaggc cacgaaaggt
     5461 cctaaggaaa tcttgattgt taaaggggca aaacacgcca aatcctttga aacaaaccca
     5521 gaacaatacc agaaaaaaat tgccgctttt ttgaaaaaag ttgagaaata agtattgaca
     5581 gttaaactca cagttgctat aataatttat gttactgatg agtagcgggg atggcggaat
     5641 tggcagacgc gcaggactaa ggatcctgtg accgctttag gtcgtgtggg ttcaagtccc
     5701 actctccgca taatgtaaga ggtgaatcat tgattcgtct ctttttttgg ttaaatgaag
     5761 gataagaagg catagattta ctggagaaaa cttttgataa tctgcttttg gaaaacgctt
     5821 taagcttgtg aaagactaga aatttatgga aaattgtgct agaatgaaca atgtaaacgg
     5881 ggagttatcc cgaaaaataa gaggaggcct tacaaatggc aatcgtttca gcagaaaaat
     5941 ttgtccaagc agctcgtgaa aacggatatg ctgttggtgg atttaacaca aacaacttag
     6001 agtggactca agcaatcttg cgtgcagcag aagctaaaca agctccagtt cttatccaaa
     6061 catcaatggg tgcagcaaaa tacatgggtg gttataaagt atgtcaatct cttattacta
     6121 accttgttga gtcaatgggt atcactgttc ctgttgctat ccaccttgat catggtcact
     6181 acgaagatgc tcttgagtgt atcgaagtag gttacacttc aatcatgttt gacggttcac
     6241 accttccagt tgaagaaaac cttgctaaaa ctgcagaagt tgttaaaatt gcacatgcta
     6301 aaggcgtttc agttgaagct gaagttggta caatcggtgg tgaagaagac ggtatcatcg
     6361 gtaaaggtga gctcgctcca atcgaagatg ctaaagcaat ggttgaaact ggaattgact
     6421 tcttggcagc tggtatcggt aacatccacg gtccataccc agaaaactgg gaaggtcttg
     6481 ctcttgacca cttagaaaaa ttaacagcag ctgtaccagg tttcccaatc gtattgcacg
     6541 gtggttctgg tattcctgac gatcaaatca aagaagcaat ccgtcttggt gttgctaaag
     6601 ttaacgttaa cactgaaagt caaatcgctt tctctaacgc aactcgtgaa tttgctcgta
     6661 actacgaagc taacgaagca gaatatgatg gtaaaaaatt atttgaccca cgtaaattct
     6721 tggctccagg tatgaaagct gttcaaggtg ctgttgaaga acgtatcgat gttttcggat
     6781 cagctaacaa agcataattt agagtattaa ataccctaaa gccctcttga gggctttttt
     6841 agatacaatt gacaccctaa aatggatacc agtttctgtg aaacaatctt tgattgtgaa
     6901 caaacaaagc ataatttaga gtattaaaat ctggaccctg ctatctatgg taagtctttt
     6961 tgttattgtt aaagaggtta agcggaatgt taatatgttt tagctaaaaa atgtagtgaa
     7021 aaatgagtac gtagactatt gtaatagtaa ttccgtaaaa ttgttgaaaa agaaaatggg
     7081 aaataccttg tcaaattaag caccctatga tagaatagat aggtatgtgg tgctagcaca
     7141 tccgtaaaca tattctacgt aggaggaaac agatcaaaat ggctaaagta tgttatttta
     7201 caggacgtaa aaccgtttct ggaaacaacc gttcacacgc gatgaaccaa acgaaacgta
     7261 cagttaaacc aaaccttcaa aaagttacta tccttgttga tggtaaacct aaaaaagttt
     7321 gggcttctgc tcgtgcgctt aaatctggta aagtagaacg catctaatag aaataagcct
     7381 tagggctttt tttattagct tatttctatc cataagctag tcagggtaat aaacgctatt
     7441 gttaactatt ctatctcttt tacattactt ctaaaatatg ataaaatagg atgataaaaa
     7501 ctttttgagg tagtaactat gactgtaaaa attaatacaa aagatggtct aatcgagcta
     7561 tccgacgatg tgattgcaac tgtcgtggga ggatcagcaa cggaaatttt tggtgttgtt
     7621 ggaatggcca gcaaaagtgc aataaaagac aattttcaat cactacttcg caaagaaaac
     7681 tacgctaagg gtgttgtagt aaaatcaaca gatctgggta tttctgttga tgtctacact
     7741 gtgatgagtt atggtgtgaa aatcagtgaa gtctctaaaa atatccaaga acgcgtgaaa
     7801 tttaaccttg aaagtcagct tggtctcact gctgatatgg tcaatgttta cgtacaaaat
     7861 attaaggttg taggagaaaa ttagtgtcaa atattacaac aagcttattc caagaaatgg
     7921 tccaagcggc agcgactcgc cttggcaaac aagcagaata tgttaattcc ttaaatgttt
     7981 ttcctgttcc agatggtgat acaggaacaa acatgagcat gactatggac aatggtgcca
     8041 aggaagttgc tgataaacct gcgtcaactg tgggagaagt tggacaaatg ttatcaaaag
     8101 gtcttttgat gggagcgcgt ggaaattctg gagttattac ttcacaactt tttcgtggtt
     8161 ttggtcaaag cattaaagga aaagatgagc tgacaggtaa agatttggca caagcctttc
     8221 aagtgggtgt agaagttgcc tacaaagcag tgatgaaacc tgttgaagga acaattttaa
     8281 ctgtttctcg gggtgcagcg actgctgcct taaaaaaagc agatcttaca gacgacgccg
     8341 tggaagtcat gcaagctgct ttagacggtg ccaaaggtgc ccttgctaag acacctgatt
     8401 tattaccagt ccttaaagaa gttggtgtgg ttgattcagg cggtcaaggt cttgtcttta
     8461 tctatgaagg tttcctatct gctttgaatg gcgactatgt gacttcagca gactttaaag
     8521 cgactcctgc taacatgtct gaaatgatta atgctgagca tcacaaatcc gttgttggtc
     8581 atgtggctac tgaagacatt acctacggtt attgtactga aatcatggtt gcccttaaac
     8641 aaggcccaac ctatgtgaaa gaattcaact atgatgagtt tcaaggctac cttagtggcc
     8701 ttggtgactc gctgttagtg gtcaatgatg atgagattgt taaagttcac gtccatactg
     8761 aagaccccgg acttgtgatg caagaaggtc ttaaatatgg ttcgctcatc aagattaaag
     8821 tggacaatat gcgtaaccag catgaagcac aggttcaaaa gactgatgtc gaaaaaaaca
     8881 aggctgaagt aaaagacttt ggcttgattg cagtagtagc tggagaaggt ttatctgaaa
     8941 ttttcaaggc gcaaggtgtt gattacgtga tttctggtgg tcaaaccatg aatccatcta
     9001 cagaagatat tgttaaagct attgaagcag ttaatgccaa acaagtcatt atcttgccaa
     9061 acaataaaaa tatctttatg gctgcccaat ctgctgcaga agtggtggat attccggctg
     9121 ctgttgttgc aacacgtact gttccgcaag gatttaccag cttgttggcc tttgatcctt
     9181 caaaatcttt agaggacaat gtagcagata tgtcaactag tctttctgac gtggtcagtg
     9241 gaagtgttac tttggcagtc cgtgacacca ccattgatgg tttagaaatc catgaaaatg
     9301 atttcttggg aatggttgat ggtaaaatca tcgtttcaaa cccagacatg gaagcaactc
     9361 taaaagctgc ctttgaaaag atgattgacg aagatagcga gattgtgact atctttgttg
     9421 gtgaagaagg tgaccaagac ttagcagaag aacttgctgg gtatttagga gagacttacg
     9481 aagatgtgga agttgagatc catcaaggag atcagcctgt ttacccatat ctcatgagtg
     9541 tagaataata gaatacaata aaaaaagagg agttaggcaa aggtctgact ctttttgtct
     9601 atattgatgt cgtaatgagt ggttttagtg atggtaagcc cctaaaaaat gagggtaatc
     9661 catcacaaag ctaatggtta cgatttaatt tgacaaaacc tagaaaaaca ttataattgt
     9721 attataacaa taagtcaagg actgaaaacg gttcagttct aagacattac aaggaggaga
     9781 ttttatgtta ggaccattta tctttattgc ttttggggtt attgtaatcc tagctattgt
     9841 tgccagtacc ctttatgttg ttcgtcaaca atctgttgcc atcgtggaac gttttggtag
     9901 gtaccaaaaa acagccacta gtggtattca tgtccgcctt ccgtttggga ttgacaaaat
     9961 tgctgcgcgt gtgcagctgc gtcttttaca gtcagaaatc atcgttgaaa ccaaaaccaa
    10021 ggacaatgtg tttgtaactt tgaatattgc cactcagtat cgtgttaacg aacaaaatgt
    10081 aacagatgct tactacaaat taatgaaacc tgaatctcaa atcaagtctt atattgaaga
    10141 tgccttgcgg tcatccgtgc cgaagttgac cttagacgaa ttgtttgaga aaaaagatga
    10201 aattgccttg gaagtacaac atcaagtggc agaagaaatg tcaacctatg gatatattat
    10261 tgtcaaaacc ttgattacca aggtggagcc tgatgctgaa gtcaaacagt caatgaatga
    10321 aatcaatgcg gctcaacgca aacgtgtagc tgctcaagag ttggcaaacg cagataaaat
    10381 taaaattgtg acagcagcag aagcagaggc tgaaaaagat cgattgcacg gggtagggat
    10441 tgcccaacag cgtaaggcca ttgtggatgg tttggcggag tctattcaag agcttaaaga
    10501 agcgaatatt tccttaaacg aagaacaaat catgtccatt ttgttgacca accaatacct
    10561 ggataccctc aatacctttg cggcaaaagg aaaccaaacc ctttttctac caaatacacc
    10621 aagtggggta gaggatatcc gtacacaagt tttatctgcc cttaaaacaa aataacgtgt
    10681 aaaagcttgg ctaaaaagtt gctcaatact ggatttagca gaaggctatc ataccttgtg
    10741 aagtggcaaa cgacgaaatg aatacattac ttttattgga gaaatgaaag taaaaaaagc
    10801 ctttttcatg attaacaata tagtattgat taaatgttaa gttgtattga cttttaaatt
    10861 aaataagttt aaaatcaact taataagcaa aaaggagatt ttcaatgaaa tccaaaaaag
    10921 ttgttagtgt tatatcactt accttatccc tttttttggt gacaggttgt gctaaagttg
    10981 ataacaacaa atcagttaat cgtaagcctg ctactaaaca aacctataat agctatagtg
    11041 atgaccaatt aagatcgcgt gaaaatacca tgtctgtttt atggtaccag cgagctgcgg
    11101 aaactcaagc gctttattta caggggtatc agttagcaac ggatcgctta aaagaacaac
    11161 tcaataaacc aacggataaa ccttattcaa ttgtattaga tattgacgaa acagttcttg
    11221 ataatagccc ttatcaagct aaaaatgttt tggaaggaac aggatttaca cctgaaagct
    11281 gggattattg ggtacaaaag aaagaagcaa aaccggttgc tggtgctaaa gactttttgc
    11341 aatttgcaga ccaaaatggt gttcaaattt actacatttc agacagatca actactcaag
    11401 tagatgctac aatggaaaat ctccaaaaag aaggtattcc agtacaaggt cgtgatcatc
    11461 ttctattctt agaaaaagga gtaaaatcaa aggagagtcg tcgtcaaaag gtcaaagaaa
    11521 caactaatgt aacgatgcta tttggtgata atcttctaga ttttgctgat ttttcaaaaa
    11581 aatctcaaga agatagaaca gctttattat cagatttgca agaagagttt ggaagacgct
    11641 ttatcatttt ccctaatcct atgtatggtt catgggaagg tgccatttat aaaggtgaaa
    11701 agctggatgt gcttaagcaa ctagaggaac gccgtaaaag tttaaaaagc tttaaataaa
    11761 gtaattgaaa aagaggtgca aaagaaaatg taacctcttt ttaa
//
LOCUS       DAUCWO010000053        11759 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUCWO010000053 DAUCWO010000000
VERSION     DAUCWO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.053"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1573
                     /locus_tag="VGY20_001337"
     CDS             <1..1573
                     /locus_tag="VGY20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274186.1"
                     /translation="YLVSLLLGPTLYFLAVLAITHLLELTLEVQWFITANEIIESLGI
                     LVVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAITAITAITALLLSYIL
                     CLATVFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKENKKRLYRP
                     LSNIIYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEY
                     NVESENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKE
                     LMKAQGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTS
                     VTTLVVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYT
                     VKTANIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRI
                     LRYETGILFLIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGT
                     FYVLTLRSVTSIIKN"
     gene            1613..3175
                     /locus_tag="VGY20_001338"
     CDS             1613..3175
                     /locus_tag="VGY20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ4274187.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     IILLYCYTVEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNSILFNFLFLMVFTI
                     LHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGW
                     YLFPLIMSVIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFL
                     LFFIVVDASTLFCLVVISCIFYYLKDSKQNFDYENRRLNYFMKSEENMRVEFSNYLHD
                     DVLQNIIAIKNLLSLENSNITHGFIVNELNDLVSGIRDEMDTYHPIVSANQTMKENIQ
                     SLFDDIVKSRKSNTLLYFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSL
                     TIQSDIIIIEESNQVVEKVHSINYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLP
                     IDWKLCYEDFIN"
     gene            3156..3761
                     /locus_tag="VGY20_001339"
     CDS             3156..3761
                     /locus_tag="VGY20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4274188.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(4314..5894)
                     /locus_tag="VGY20_001340"
     CDS             complement(4314..5894)
                     /locus_tag="VGY20_001340"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ4274189.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLDQHFNTFTEAQKIFEDAYQDEDLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTIANVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDNHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(5907..7082)
                     /locus_tag="VGY20_001341"
     CDS             complement(5907..7082)
                     /locus_tag="VGY20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ4274190.1"
                     /translation="MTKSKQPQYRFDGFKGEWEEKELGDIVQITMGQSPSSQNYTTNP
                     SDYILVQGNADIKNGYVFPRVWTTQITKQADKGDIILSVRAPVGDVGKTNYHVIIGRG
                     VAAIKGNEFIFQILKYLKEIGYWKRISTGSTFDSISSSDIKYAKIQIPSLPEQEAIGE
                     LFQTVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFDGEWEEKKLGEI
                     SRMFSGGTPNVGIPEYYNGNIPFIRSAEINSDQTELSITDKGLSNSSAKLVEKNTLLY
                     ALYGATSGEVGLSRISGAINQAILAIIPEKKYSSLFIKNWLYKQKSSIIKKYLQGGQG
                     NLSGSIVKELTIHFPSLSEQEAIGDFFQTLDQQMSQTEDKLTELKALKQTLLNRLFV"
     gene            complement(7095..10073)
                     /locus_tag="VGY20_001342"
     CDS             complement(7095..10073)
                     /locus_tag="VGY20_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ4274191.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     AIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPLAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSCWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFLEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAYQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRRDDDEAIPEINIDYELESIQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIVYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            10354..11496
                     /locus_tag="VGY20_001343"
                     /pseudo
     CDS             10354..11496
                     /locus_tag="VGY20_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
BASE COUNT         3626 a         1913 c         2193 g         4027 t
ORIGIN      
        1 ttacttggta agtttattgc tgggaccgac tttatatttt ttagctgtac tggcaattac
       61 tcatctattg gaattaacat tggaagttca gtggtttatt acagctaatg agattataga
      121 gtctttagga atattagttg tagtttttct gattaatgtc atcacaaatg gacttatcat
      181 tagtaaacag tctttgattg aatttgttaa tttctcaaga aaggctgaga aaaaaattaa
      241 gataagaaaa gtcagagcta ttactgctat cactgctatc actgcattgc tattgtcata
      301 tattttatgt ttggcgacag tgttttcatc cacacgaaat atgctattaa gcatagggat
      361 ggtaccggtt tctctattga taattgtctt agttgtttta ggaacagtgt tcaccatcag
      421 atatggattg gcttttgtag tttcgttgtt aaaagaaaat aaaaaaaggt tataccgtcc
      481 tctgtctaat atcatctatc ccaaatttaa ctatcgtatt gcaacaaaaa ataaattatt
      541 aacagtcttg ggaggtcttt taacagtaac cgtttcagtt gccggaatga tggtaatgct
      601 ctatgcttat tctcttaatg ggatagagag gttgactcca tctgccatag aatataatgt
      661 tgaatcagaa aacggtcaag tcaatgttac aactatttta gagaacgacc aagtgagctt
      721 ggttgatgtt gacctattga gattgaacac taacccagaa gtaactatca cagaatctgg
      781 gcaaacaatt ccttattttg atataattaa ttacagtgat tataaagagt taatgaaagc
      841 tcaagggaga ataaattcta ttgaaggtag taagtcactc ccattgttaa taaattatta
      901 tccaacagaa attagccttg gaaaaacctt taacttagga aatgcatatg atgttactgt
      961 aaaacaagta tcaacgcata atgtttttag tttttctaca agtgtcacga ccttggttgt
     1021 ttctgataaa ttatatgcta aacttagttc ttgttttcca gagaaagaaa tgacaattag
     1081 gacttttaat ggaaattcga ttaggtcaag tgaagcattt tacaatcagt ttagtatggt
     1141 tcctgatgtt atcagtagtt atagtaagga atacacagta aagactgcta atattgcgac
     1201 ttatatcttt ataactttcc tatccatact ctttattatt tgtacaggta gtattctgta
     1261 ctttacaagc ctcatcgaaa tcatggaaaa taaagaagaa tatggctatc taagtaagct
     1321 aggttatagt aaaaaaatga ttcatcggat tcttcgatat gaaacaggta tacttttcct
     1381 tattcctgta ttcattggga ttgtaaatgg tggtatgttg cttatttact ataaatattt
     1441 attcatggat acattggtag caggcaatat cataatgtta tctttattgc tttgtctgct
     1501 tttcttcttg ataatatatg gcacatttta tgtattgaca ttgcggtcag tgacatccat
     1561 aatcaaaaat taattttaaa aatcttttaa ctttaacgat taagtaaata atatgataag
     1621 aataaaaaac ataacaaaga gtaagttctt tgggactgca ataatcttgt tgcaacaatt
     1681 aatagccttg ttaattttgg tctataatcg agagaacctg tctcttcttt tctctgaaaa
     1741 agtagctctt gtaatgaccc ttattgatac agcttttatt tggctggcaa caatattacg
     1801 ccaaaaacaa ggtgatattt ttaaaaggat tatttctatc atctccttaa caatttggca
     1861 atacttagta tctgtgctga caagaggaac tcctctcttt ttaagcagtg gactacagat
     1921 tatattgctc tattgttata ccgttgagat tactaattta atattatatg ggcacaaaca
     1981 atttaaagat aagttagata agggcttgtt aataattatt tttgtttcta taactagcct
     2041 ttttgttaat agcattttat ttaatttttt attcctgatg gtttttacta ttttacacct
     2101 gtatccgtta ctagttatag tgctatatta taggtccttt aggcaacaaa tatcagttgt
     2161 tagacgttca ttgatactat tttctttact tttgctggta atacttggta gtgaacttta
     2221 tggagaaatg ctagatgtta atcaagcctt taataatttg ggatggtatc tgtttcccct
     2281 tataatgagt gtaatatact attttaagac catacatgat aaattgagtt ttgttaccca
     2341 aagatggtta ggtgattaca aagctcgtat agaactatta tttctacttc ttatcctttg
     2401 ttggattgta ctcatgaagg tattagttaa agattttttg ctatttttta tagttgttga
     2461 cgcaagcaca ttgttttgtc tagtggtaat ttcttgcatc ttttattatt taaaagactc
     2521 caaacaaaat tttgactatg aaaatagaag actgaactat tttatgaaat cagaggaaaa
     2581 catgagagtg gagttctcaa attatttgca tgatgatgtt ttacaaaata tcatagcgat
     2641 aaaaaacttg ctttctctag aaaatagtaa tataacacat ggttttattg ttaatgagtt
     2701 gaatgattta gtttctggta ttagagatga aatggatact taccacccaa tagtttccgc
     2761 taatcaaaca atgaaagaaa acatacagtc tctttttgat gatattgtta aaagtagaaa
     2821 aagtaatacc ctattgtatt ttaactgttc agataatatg gtagtgccat ctccatatgg
     2881 agatattgtt tataggttta taaaagagct aataaacaat gctataaaat atggagatgg
     2941 aaaagacatt cgtttatctt taacgattca atctgatatt attatcattg aggagagcaa
     3001 tcaagtagtg gaaaaagtac actctataaa ttatggtaga ggattgaaat catttcaaga
     3061 aacactagca gcttttgatg gagatctaga actacaaatg gatactaaac aatttactat
     3121 cagaatatta cttcccatag attggaaatt gtgttatgaa gattttatta attgatgacc
     3181 acagactatt tgcaaagagt atccaactgt tgttccaaca gtatgatgaa gtagatgtca
     3241 tagatacgat tacttctcat tttaacgatg taacgattga tttgtctaag tacgatatta
     3301 tattgcttga tattaattta acaaatattt ctaaagaaaa tggattggag atagcaaaag
     3361 aactcataca gtctactcct catttgaaag ttgttatgtt gacaggatat gttaaatcaa
     3421 tctatagaga gagggcaaaa aaagtaggag catacggttt tgtagataaa aatatagacc
     3481 ctaaacaact catttctatc ttaaaaaaag tggattctgg aaaaaagtat tttgaacaaa
     3541 tcgaatctca ggattatgtt gaatctttaa ctgaccagga aatagctatc ttaaatttga
     3601 gtaagaaagg attctctata aaagaaatag aggaaacgtt acaaattagt aggagaacag
     3661 tatttaatca tttgacccac atctactcaa agcttttggt aaataataag caagaggcta
     3721 tctacaaagc cgaacaatta gggtatttta tggatttctg agttgcagat aatcaaacga
     3781 cataagtttt acttgtgtcg ttgctaggga tttcctgtta aatacccaga tttaaaatca
     3841 ttgtttttta agttcttttt gattgaaatt gattgagata acgcttttat aaaaagtagg
     3901 gcaatgcctg atatgaaaag ctttttaaat ttagtcaaac aaaagttgtt taaaccaggt
     3961 ctaaaaaaac tcgtaaagct tcacaactcc cagaacgtta atatatgctt atatatcaac
     4021 gattggaact actttatggt tcgcaccatg gtttttgtgg aaggatcaaa agttgtcctg
     4081 aaaatttctc ttaaccgtgt ttaagctttc taaacgtttg agaaatcatt gtaacttcaa
     4141 ataactattt taaataaaac aaagaaacgg acaaagttag atgagatgat tctattttga
     4201 tttataggtg aagaaggaga atcttgtttg ataagtactc aataaaggtt cactactcaa
     4261 ttttaaataa aacgccacag tagtaatcat ctactgtggt gttttttggt cttttagttt
     4321 ctaaaagtat ccaattcgtc ttgtgcttct ttggttgttc cgactagttg tttcatgagt
     4381 tgatctagtt tggcatttgt tttggcaatt tcttgatcaa tgtcactgag ctgtttagct
     4441 aattctggca gtggtttaac tgggacttcc tcaaaggtat caacatagcg tgggatattt
     4501 aagttatagt cattttcaat gatttcatca aatgaggcta aatatgaaaa cttatcagaa
     4561 ttatcacgac tcttataagc atcaaggatc tttttgatat ggttatctgt cattgtgttt
     4621 tgatttttac ccttatcaaa ctcttttgaa gcatcgataa agaagacatc tttgttggtt
     4681 ctgttcttct ttaatatgat aatagtcgta gggatgcttg tattataaaa gatatttgat
     4741 gggagtccga taatggtatc gatagcccct tgttcgagta gtttttggcg aatcttgcct
     4801 tctgcagctc cacggaaaag gacaccgtgt ggtaagacaa tcgccatagt tcctgtattt
     4861 tttaaatgat agaaaccatg aagtaaaaag gcaaaatcgg cttttgattt tggtgctaat
     4921 acaccataac ttgaaaaccg tggatccgtt aagaaaccag cagtcgctga ccatttcaaa
     4981 gaataaggtg ggttcatcaa caccccatca aagttggttg gttcatctgt tggccagtct
     5041 gcatccaagg tatcagcgtt acttaagtgt tggttctcga tggcaacgcc atgaagcatc
     5101 atgttcatac gagccaagtt ataagtagaa gtattgattt cttgaccata gtaagaaacg
     5161 gtatcggatt ggttactata tttcttagca tttaaaagga gtgatccaga tcccatagca
     5221 gggtcataga gggtcattcc tttttgatct tcacgaccta aaaagacaat ctgagtcatt
     5281 aagtgagaga cagcttgtgg ggtatagaac tcacctgctt tttttcctga ttcactggca
     5341 aattcaccaa tcaagtactc ataagcatcg cctaaagtat ccccatctac tgcctcaaag
     5401 tcaatttcat ttaatgtttt catgacattg gcaatggttt gattttgttt ttgaggagta
     5461 gagcctaatt ttttagagta gaggtcaata tcttcaaata agttttcaaa gtcttcacca
     5521 ctttgttcaa tatcgcgaaa accttgagct aatgattcta attggaaagt attgtgataa
     5581 acatcttgga tgagtttttc aaaggtcagt gtcggttcga taaagtaacc taaatcacca
     5641 gtaacaacag aaatgaggtc atcttttagg tcttcatctt gataagcatc ttcaaagatt
     5701 ttttgggcct ctgtaaaggt attaaagtgt tgatctaaat tatcacaaac agcaaagagt
     5761 aatttatcag ataaatgttt gtaaaaaata agtccaagaa gatagttttt atagtcattg
     5821 gcatccattt ggccacgtaa ttgatcggcc gaatgccata gtgcttgccg gagtgaagtt
     5881 gttttctctg ccatgtgagt cctttcttat acgaataaac gattgagtaa tgtttgtttg
     5941 agtgctttga gttctgttaa tttatcttct gtttgactca tttgttggtc taaggtttgg
     6001 aagaagtcac cgatagcttc ttgttcggag agagaaggga aatgaatagt taattcttta
     6061 acaatagaac cagataaatt tccttggcca ccttgtaaat atttcttaat aattgaggat
     6121 ttttgtttat ataaccaatt ttttataaaa agagaagaat attttttctc aggaataatg
     6181 gctaaaattg cttgattaat agctccactt attcttgaaa gtccaacctc tccactagtt
     6241 gcaccgtaga gagcgtataa taatgtattt ttttccacta attttgctga tgaatttgat
     6301 agacctttgt ctgtgataga taattctgtt tgatcactgt tgatctcagc agatctaatg
     6361 aaaggaatat taccattata gtattcaggg atacctacgt taggagtgcc acctgaaaac
     6421 atccgagata tctcccccaa tttcttctct tcccactcac catcaaaacc ttgtaaacgg
     6481 atttcaggga ctttttgtcc ttgagctggg aacattttcc tgaggaaggt ttgcttttgt
     6541 tctttaaggg ttgctagctt ttgatcctgc aactgaatta attgatcaac cgtttggaag
     6601 agttcgccaa tggcttcttg ttcagggagg gagggaattt gaattttagc atatttgata
     6661 tcactagagc taatgctatc aaaagttgaa cctgtactaa ttcttttcca ataaccaatt
     6721 tctttcaagt attttaatat ttgaaagata aattcattac cttttatagc agcaactcca
     6781 cgcccaataa taacatgata atttgtcttt ccaacatcac caactggagc cctaacacta
     6841 agaataatat cacctttgtc agcttgtttt gtaatttgtg ttgtccagac acgcggaaaa
     6901 acatatccat tttttatatc ggcatttcct tgtactagaa tataatcaga agggtttgta
     6961 gtataatttt gacttgatgg agattggccc attgtaattt gaacaatatc ccctagttct
     7021 ttttcttccc actccccttt aaaaccgtca aagcgatatt gaggctgttt agattttgtc
     7081 atctctttcc tttcctagtg cccttttaag ggttggattt gatctctaat gaattgaact
     7141 aactcccgtt caaattggct cttgtatttg agtttattca tggccgagtc accttgtttt
     7201 tctttatagg cttggtagtc tagggattct tttaaggttg acatgcccac ttgtttggta
     7261 gagttagcac tatgataatt agtcgccaca aaggctaatt tgtcaggatc tgccttccat
     7321 tggtccgcaa agtgtttgat ggccctttgg atcttatcac caatgaggtt atctaactca
     7381 taaacaatgc ttttcccttc ataggctttg ggttcttttt ggatatcttg ccataactca
     7441 gctagtgagt cagccatagc agggtttgat tttgccaaat cttggatgta ttgatccatg
     7501 ggattgtcat tgagcctttc ttgaagagct tcttgttctt gatcgacaaa ggcttgaatt
     7561 aaggtcaaga tatagtgata gttgatatca tccatctgaa tactttctag ctcatagtcg
     7621 atattgatct ctgggatggc ttcgtcatca tctcttcgtt ttcgtatttc tgcaatgaca
     7681 ttttggtaag tgcctttata agtatcaatc acttcatctg ataacccaac ttcactcaag
     7741 agtgtttcat taaagtcact atagacctgt acagatgcat agagcttatc aaatgcttga
     7801 taggctttgg cgtattgttt gagaaaagct gtctgtgcgc tctcaatagt tgggaaagcg
     7861 tctggatcag gaactatatt ttttaatact gttacttttt caaggaatct tgctttttct
     7921 tcttcccaag aaggggccaa aacgtcgttt tcacccccat tagagtagag gctaagcgct
     7981 ttgtcgacag cttctttaaa acgaagtggt gtttggaaag tcacaacctg accgtaatgt
     8041 tttctacttt caaagatacg gttggttcgt gagaaagctt gaataatgtg ctgaggtttc
     8101 ataggctggc gatcaataaa gatcgttgat aaacaaggcg catcaaaacc tgttaagaga
     8161 cggtcaacca caatcacgag gtctaattgt tcatgacggt ctttgaattt atctttttta
     8221 cgggctaagc gatcatttaa atctctgtta tagccttgta agttatcgat ggtaaagtta
     8281 gtgccaaaga ggtgattata gtcttctaga tttttggtca ttttatcttg tcggctgata
     8341 gacgcattgt cattttcagt gatagaataa gtaatagcta cctttggaaa gtctggtagt
     8401 ttttctttga ccttcttaga aatgactaaa tccgtttcac cagctttaac tttctgaatc
     8461 agatcataat aggcttgggc acgagcaata ctcttaacgg ttaataagcc ttcgaatgtc
     8521 tgaccaatcc cattattaaa gccaagcttc ttacgtgatt gattaatgat actatttaag
     8581 acagctaaca tatgttcttc atgatcataa gcttcttcag gaatactgtc ttcaggcata
     8641 tcaggaatgg ttgtcttata ctcaacttgg aagcctagaa ccgctttatc gtgaatggct
     8701 tctttgacag tgtattgatg gagacatttg ccgtattgct gttccgttgt ttgggctaaa
     8761 tcacctaatt gagcacgctt attttccaca aagataggag tacctgtaaa gccataccag
     8821 caactgtttc gaaaagtatt cgtgagatga cgctgccgtt ctggcgtgac agcacggtgg
     8881 cattcgtcta caacaaagac gacatttagg tgtgcaagac gctcttttag tttcttgaat
     8941 tttggagtgt cataactttc catctgactg atcatagcat tgagtttttg aatggtggtg
     9001 accacaacac gacgatcact ggattctaaa tttttgatga gttgtctcgt atcttctgtc
     9061 tcatcgacat caaagatatc attttgcgca taagattgaa aagcagaagt ggtctgatta
     9121 tctaaatcct ttcggtcaat gacaaagatg gatttttcaa cggccggtat ttgaaggata
     9181 ttgcgagcca ctttatagga agttaaggtt tttccagaac ctgttgtgtg ccagatatag
     9241 ccagacttgc ggtgacgact cgcttcagcg acggcttcga ttgcatgaat ctgataagga
     9301 cgaaggagaa ttagtgcttt tttatcatca tcaatgacag agtaagtcat gaccatttgg
     9361 tgagcaagag ggatagatag gacttctttg gcaaaagcaa agagatcttt ttgtggtttg
     9421 ttgttttgat ctacccattg ggtcaagaag ttaggattta atttattttc tttagctgca
     9481 gcgatatacc ttgtatctgt tttatttgag accacaaaca tttgtaaggt tgaaaagata
     9541 cctctaaact ggccttcttt atcatacttt ttgacttggt taaaagcttc aatgcattga
     9601 tgattctgag attttaattc aatttggatc ataggtaagc cattgatgag aagtgtcaca
     9661 tcacctcgtc tatcacgaga accagagaaa gcaacttgat tggcaatctc ataaacagaa
     9721 gttccaccag ccacattgtc tgctttaacg acttctaaac gaatcgctcc tagttttgca
     9781 tcttcgcgtt gcacttgtac tttagcaatg ccgttttcac cagctaacca tttagccgct
     9841 tcatagtaat tgacaaagtt aagttgattc ttaatctgct ctttttcact agctgttaaa
     9901 ggctcctcat tcagatattg cgtgttattt tgcgctaaga tcttaaagaa attatcccat
     9961 aaagcatctt cagtttttaa ttctttacga tatgtccatt gactttcacc tgtttctaag
    10021 agatggatca actctttttc aagttcaagt tccgttttag tttgatttcc catttttaat
    10081 ccccattcga catgatatag tagtattata tcattggcgg atccttttta gaatggcaaa
    10141 gctattgaaa ccttccaaac agtctttttt tgattagttg aatgcttaag atcctagcaa
    10201 ctaaacaaag ctatgactat gtaaatgcaa tcgagaaagt tcttgtattt ttgagtttgc
    10261 ttgacaataa tttaaggtga agaaagtaat aagacaacac tagtaaaaag gtcgagttct
    10321 caaaatagat aaaaaactta agacagatct agcttgacta ttaatagggt tttaaaggac
    10381 gataatctgc tacaatatac agaaaaggaa tttattgaag atatgaaaat tttggtagcc
    10441 atagattctt ttaagggatc ggtaacgtct tcagaactta acactagtgt agctcaagcc
    10501 cttctttctg tcgataagca gttagttatt gaagcaagag ctatcgctga tggagaagaa
    10561 ggaagtttag cagccctctc acaaaccgtg gcggggaggt ggcatcaagt aaaaaccgtt
    10621 gatctattaa gacgcccgat taaagtcgct tattatcgtc acgcaaagca agcttttatt
    10681 gaatctgcga gtattattgg tattgatagg atcaccccca gttcagtaac ctatgcacaa
    10741 gccacttcct acggtttggg tttggcagtt aaagatgcca ttcaaaaagg agctactcaa
    10801 atcgaaatca tgctaggagg aacaggcact tctgatggtg gcaaagggtt tttagaaagt
    10861 ttaaactatg atttcatgac aggaagatct tatcttgata ctttagcatc acctgttacg
    10921 ctattaggtc tgactgatgt aaccaatcca tatcatggcc ctcaaggatt tgctgcggtc
    10981 tttggccctc aaaaaggtgg gagtcttagt caaattgaag aaacagatca aattgcaagt
    11041 aactttgcta aaaaagtttt ttaccaaaca accattgatt tacagacaat accaggaagt
    11101 ggagcagctg gaggattagg aggtgccatt gtcctattag gaggcacctt aactagtggt
    11161 tttagcagga ttgcagagct actaaatctg gataattccc ttcagtcttg tgatttagtt
    11221 attactggag aaggatgcct ggatacacaa tcacaatctg gcaaggtacc tgttgcgatt
    11281 gcaagaatgg ctaaaaaata ttaagttcca acgattgctc tatgcggttc tgtaaagatt
    11341 gagacaggac tagcagcaga agactttttg gctgtttttt caatacaaca gcagccaatt
    11401 tccttagaag cagcgataga taaaataaca acactttcta atatcaaaat attagcagct
    11461 aaccttatgc tattgatagc tcagtttaat aagtaaactc atctattttc gattgtgtag
    11521 cattcatttg gcggttaaaa gtaacctatg aagaaggaga cgcacgagct aactcattag
    11581 catagtatga aaaacagaaa cattttgtaa aaaagagttg acaatgacag taaagatatc
    11641 gtatactaat aaagctgtca gagaggttgc tgtaacacac ttattgaaaa aagatgaaaa
    11701 agtagttgac aggctatgag aaacctgata gaataaagaa gttgtctctt aggagacgt
//
LOCUS       DAUCWO010000054        11482 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUCWO010000054 DAUCWO010000000
VERSION     DAUCWO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.054"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(387..2963)
                     /locus_tag="VGY20_001344"
     CDS             complement(387..2963)
                     /locus_tag="VGY20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ4274192.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLASMP
                     DAIYLFTLIKFGVIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMVALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPSTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFSSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNINTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYISIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(3046..4665)
                     /locus_tag="VGY20_001345"
     CDS             complement(3046..4665)
                     /locus_tag="VGY20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4274193.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4744..5616)
                     /locus_tag="VGY20_001346"
     CDS             complement(4744..5616)
                     /locus_tag="VGY20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4274194.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5739..5980
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6035..7057
                     /gene="trpS"
                     /locus_tag="VGY20_001347"
     CDS             6035..7057
                     /gene="trpS"
                     /locus_tag="VGY20_001347"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ4274195.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     gene            7365..8846
                     /gene="guaB"
                     /locus_tag="VGY20_001348"
     CDS             7365..8846
                     /gene="guaB"
                     /locus_tag="VGY20_001348"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ4274196.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
     gene            9049..9912
                     /locus_tag="VGY20_001349"
     CDS             9049..9912
                     /locus_tag="VGY20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ4274197.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWIFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTKPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFDVLSVILPMAFGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
     gene            complement(9968..11074)
                     /gene="recF"
                     /locus_tag="VGY20_001350"
     CDS             complement(9968..11074)
                     /gene="recF"
                     /locus_tag="VGY20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ4274198.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(11076..>11482)
                     /gene="yaaA"
                     /locus_tag="VGY20_001351"
     CDS             complement(11076..>11482)
                     /gene="yaaA"
                     /locus_tag="VGY20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ4274199.1"
                     /translation="KLFTEFITLQALLKELGIIQSGGAIKRFLAETTVLFNGEDEKRR
                     GKKIRVGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKKTSS
                     KHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
BASE COUNT         3738 a         1853 c         2171 g         3720 t
ORIGIN      
        1 actaacagga ttgtattttg cattaaaata gaagttctgt tctttaattg tatagatgat
       61 aacattaagt ttaattacaa acattaactg aagcttaatt ttttacattt tgaaagcatg
      121 tctcccattt aatactgatg aatataagat tattgtaagg agtcaaaaga ttgaaatcgt
      181 atcagcgatg attattgtca tttcaatctt tgaaagagag tagtttaaca ttaaccctca
      241 cgaaaaatct gacctttata ttatttgagt ttattagtaa aatcgataaa agcatctcaa
      301 gtattagaat atttgtctag ctcttgagat gcttttagta tattgactca tttgccaaat
      361 tgataaaaaa atctgatttt tttggactaa agtaatttag actttatatc tatgtactta
      421 tacaaaagca tataagcgat aattcctacg caagatagag ataaccctaa tttaaaacca
      481 ttaggaataa aggtaaggat aacacgtccc tttcctttag gaatagtcac tgatagaaat
      541 cctccttgtg cttttttgac tggaagattt ttcccatctt tttgtgctga ccaaccttta
      601 tcgtaaggaa gtgtaaaaat aagagaacct ttcgtttttg aattataatc agtgattacc
      661 gtattacttt tagcgtaagt atgaacattt ttttgtttaa tactattcat tgcttcaagg
      721 taagattcaa tagacaaact ataaaaatga ggttccttaa aactaatttg tttatttttt
      781 ggaaaaacaa acgaaaatgt agcaactttg gcatcggcga aatatcctaa atcaaaaaaa
      841 gagtaagcgt tatctgtagt atatatgaaa ttatgattat ctgtctgaat acgcacctct
      901 ttagcatcag gatttgaaaa tataatattg ggtatgctaa tatagagttg actatgttta
      961 gggatgttaa tttgataatt aagggtaacg gagttttgat aatcagaagc ttgtgcagat
     1021 atttgtccgt taaattgatt agcaccagaa ataagttgag ctggctgtaa gttaaaatag
     1081 gttaaagatt tcccacttag ttggttaagt aatttggttt ggttatcaag ggtattgata
     1141 tttaggttga catctttgta aacattacgt gttaatatag ctaaagggct actatagtga
     1201 ttttgataaa gagtagtatt cccgctagtt tttagttttg taaatccaaa tttatttaga
     1261 ggtccttcgc taagattata ttttatacca agtagactgt ccataataat agtattgttt
     1321 tggtatctaa ggtttaaatt tgtgccttta gactgaaatc ccaatcgatc caataaagaa
     1381 ctagatagtc tatttcttac agaggaaaat tgtgaaatgc cgtaataatt aaatttcatg
     1441 ctatcgttcc ctgtttgggg aagtagtctt tccattctaa aaaaaggttg actatttttt
     1501 gacacagagt tgacaaggtt gttaatatcc tttaattgac tattatatat ctgtcgtgat
     1561 gagaatcccc actccttatt aattccttga agctggtagt aggtgtttaa ccctgattca
     1621 agaaggctaa agataagtat gaaagcagaa ataaaagtag atgggatttg agaatttcta
     1681 aacgaaaata gtgaaatagt ataacctaat aataaaaaaa cactaagtaa aaaaagagtt
     1741 aaaggtagaa aattatattg ttgagaaaag ctataaggaa gagatgtcag tataatgagg
     1801 aaaataaaag caaaacctgc ttttatttga gtcacttctt ttagtcgaga gagagtctca
     1861 catgcgagta ataggataac tatggaaaaa gaccaagcgt agcgatgcaa aaacatattt
     1921 ggtgagtgca tcccctgcca aaaaagatca agtggctgta ggtaaaaact tattataata
     1981 aaagttaaca agcaggcatt ggctaatttt atttttaaag ggatactttc taaagtaaaa
     2041 taaataacac taagcattag gggaaataat cctacgtaaa tcataggcag agcattaaac
     2101 ttggtagtat cgtacactcc tattgagagc ttagcaggta tatccaaaaa ccaagcattg
     2161 ttcgtaacta actgttttac cggggataga ttctctccat aagttgacaa atctagatag
     2221 gtaggaagta ttactagagc acttgttaaa gcagcacata ttgacacagc tgtaaaccta
     2281 acaaaagcga taaacatttt gttaaaatca ttgagactta aaagacaaac taaggcgtaa
     2341 agaatacaaa aaagagcaac catgtagcca aagtagtaat tttgaatgaa taataatgat
     2401 atcgaaagat aataagttct ggttttattt tctgtgataa gtttatttaa tccaagtaca
     2461 acaagtggaa gaagaatgaa aacatctaac caagaattta gttccatttg acttgtcaag
     2521 aagctcatta agctataaaa aactgagatg gaaatcatca agaaagcact gatttttgga
     2581 tataatctat gaaaagaata gcatgcagct aatcctatta ccccaaattt tatcaaggta
     2641 aacaaataga tagcatctgg catagaggct aaattaaaaa agaaaagtaa tggagaaaag
     2701 aaactgccaa gataataaca cattaaagca taaaaattta tccctagtcc gcttgtaaag
     2761 gtataaaaaa aactatcaga accgtgcatg atgttacgaa agttctgcgc aaaaataaca
     2821 tactgatgaa aagcatcact agctagaatt gttttatcac tattataata aatgcccatc
     2881 gatagaagga tgataaatat aatactaaga gggaacaaaa aactagcaag tccagcaatt
     2941 atccatttat tattattttt cataaattat atggtctttc gtttacaaat tttaaaaaag
     3001 aggctaggaa gagatcttct tagccacctc atttatcata aggttttatt tccaaagttc
     3061 agcaactcgg gcttggactt ctggattgtc aaggaattcg tcataagttt catctatacg
     3121 gtcgataaca ccattttttg aaataacaac gatgtggtta gcaatcgttt gaataaattc
     3181 atggtcatga ctggcaaaga taacagattc tttgaagtct ttgatcccat cattaagact
     3241 tgaaatagat tccaaatcca agtgatttgt tggatcatca agaataagaa cgtttgattt
     3301 caatagcatc aacttagaaa gcatgacacg cactttttcg ccaccggaaa gaacgttaac
     3361 agatttttta acttcgtctc cagagaaaag catccgtcct aggaaaccac gtaaaaaagt
     3421 atcgtcatct tcaccttttg aggcaaattg acgtaaccat tcaagaatag attcttccgt
     3481 ggcaaaatct tttgaattgt ctttaggcaa gtaagaacga cttgttgtca ctccccattt
     3541 aatggttcct tcgtagtcaa tatcatctgc taaagcacgc ataagagcgg ttgtttgaat
     3601 atcattttga ccaataatag cagctttgtc acctggacgt aaaatgaaac tgatattatc
     3661 aatgattttc tctccatcga tcgttacaga aagattttca acagtgagga agtcattccc
     3721 catttcacgc tctgctttaa aattaataaa tgggtattta cggcttgagg gaacaatttc
     3781 ttctaattca atcttatcaa gcattttttt acgagaggtt gcttgttttg atttagatgc
     3841 gtttgctgag aaacgggcaa cgaattcttg taattctttg attttttctt cggctttagc
     3901 attgcggtca gcttgtaaac gagcggctag ctcagaagat tgtttccaga aatcgtagtt
     3961 acccacaaag agtttgattt taccaaagtc aaggtcggcc atgtgagtac atactttatt
     4021 caagaagtga cggtcatggg aaacaacgat gactgtattt tcaaaatcaa tcaagaaatc
     4081 ttccaaccat gaaatagatt gaatatcaag accattggtt ggctcgtcaa gtaataaaac
     4141 gtcaggctta ccaaagagcg ctttagcaag aaggactttc actttatctc cattagccag
     4201 ttcactcata ttttggtagt gaaggtcttc tggaatattt agattttgga gaagttgaga
     4261 agcttcactt tctgcctccc agccacctaa ttcagcaaag ataccttcta gctcagcggc
     4321 acgaacccca tcttcttctg aaaaatctgc tttcatatag atagcgtctt tttctttcat
     4381 gatattgtaa agttgctcat tacccatgat tacaacatca atcgcacgtt cttcttcata
     4441 atcaaaatga ttttggcgaa gaacagataa gcgttcgtca ggtcctaaag agatatgacc
     4501 agtacttggt tcaatatctc ctgcaaggat tttaagaaaa gttgattttc cggcaccatt
     4561 cgcaccgatt aagccgtaag tattgccggc tgtgaatttg atattaacat catcaaatag
     4621 ttttcgatca ctaaagcgta gtgatacatc agaaactgta agcaatttgt cacctcatct
     4681 tatttatcta agcttcattg tacctaaaat agtccccttt ttcaatcaga aaagggctaa
     4741 tcactacata ggcaataaaa cgtctttgtc ttcacgtttg tattgtttag tcaaactaaa
     4801 tcctcttccc ataacttgag ctgctggcat gacaacaata aaggctgtgt cgtcaatttc
     4861 gaggattttt tcttggagag tgggtaattc gtgacttgat acaatagcca ttaacataat
     4921 tttatcagaa gtagtataac caccgcgaat aggaagttta gtcactccac gatccataac
     4981 agttgtgata tattctcgaa tagcttgata atctttggaa ataatcataa catttttgga
     5041 tgagtcaaag ccattttcca taacgctaat cacataacca attacgaaaa gtccaatcgt
     5101 agaatacatc acgtcatctg cagaaagtgc aataaatccc ataaggacac taataccatc
     5161 tacaattgtc attgcaactc caagagataa aggagagtat ttatgaagta tttgtgttag
     5221 aattccagtt cctcctgtcg aagagtttcc ccaaaaaacc attcctaaac caatgccaca
     5281 gataatacca ccaaaaatag ctgctaataa ctgattgtgt gtcagagtag gtaatgaatt
     5341 ggttgaacgg atggcaatag gatatatcca agaaccataa agtgttttaa taaaattttg
     5401 ttttccaaga aagaagtaac acattaataa taagggaata ttactagcca ttaaaaaaag
     5461 cgacggacta atgccaaata gagcttttat aacaactgca ataccaacca taccccctga
     5521 tgcaatatga ttgtcgacaa acattgtatt gaaaccaata gctgttatga atgagccaat
     5581 tgtgacaagt agtaaatcaa tccctcgttt tttcatgctt ttaatctcct ttataactgg
     5641 ttaaaaatag tagactcatt atctcttaaa tgtactgaga ccgtcaagtc atattttcaa
     5701 aaaatctggt gaacttgaca agaagtttaa aatttcatag aataaatcta aattagaaga
     5761 gtattctttt catgttttta gagagttagt ggttggtgca agctaacaac agaaaaggtg
     5821 aaatggacta atgattattt gaaaggaaac cataactttc aggttggcac cccttaccgt
     5881 gcagcttctt tcattgagaa gattgagata gtatctcgtt ttgataaagc agatgctaat
     5941 tgaggtggta ccgcgtatta cttgtaataa cgccctcacg ttttaatagc gtggggactt
     6001 tttgctatat caagagatag ttagggagaa aatgatgaca aaacctatta ttttaactgg
     6061 agatagacca actggaaaat tacatttagg tcattatgtc ggaagtctta aaaatcgtgt
     6121 ctttttacaa aatgaaaaca agtataagat gtttgttttc ttggccgatc aacaggcact
     6181 gacggatcat gctaaagaat ccgaattaat tcaagaatct attggaaatg ttgccttgga
     6241 ttacctctct gtcggtttgg atccgaagca atcaacgatt tttattcaaa gtcagattcc
     6301 agagctagct gaattgagca tgtattatat gaatctggta tcattagcac gtttggaaag
     6361 aaatcccact gttaaaaccg aaattgctca gaaaggtttt ggcgaaagta ttccatctgg
     6421 ttttttggtt tatccagtat cacaggccgc tgatattaca gcatttaaag cgaatttagt
     6481 acctgtaggt aacgaccaga aaccgatgat tgaacaaaca cgtgaaattg tgagaagttt
     6541 taatcatact taccatacag actgtttagt agaacctgaa ggtatttatc cagaaaatga
     6601 aaaagctgga cgcttacctg gtcttgatgg caatgccaaa atgtctaaat cattgggaaa
     6661 tggaatctat ctctcagatg atgcagatac cgttcgcaaa aaagtgatga gcatgtatac
     6721 tgatccaaat catattaaaa tagaagatcc tggtcaaatt gaagggaata tggtctttca
     6781 ttatttggat atttttgcta gaaaagaaga tcaagctgat atcgaagcaa tgaaagagca
     6841 ttatcaaaga ggtggtttag gagatgtgaa aacgaaacgc taccttctag atattttaga
     6901 acgtgaatta gcacctattc gtgaaagacg tttggagtac gctaaagata tgggagaggt
     6961 gttccgtatg ttacaagaag gtagtcaaaa agcaagaact gtggcagcca agactttatc
     7021 agaagtgaag tcagcaatgg gtattaatta tttttaatgt tttagagaaa aagctaagtt
     7081 agtaatgata agataattac tattatcaaa gcagaaccta atttgtcatt tctttacagg
     7141 atagtagaga ttatgtttta tatagtctta tttttgatta aaaataagac ccaaatgaat
     7201 tttttgatta ataaaaaacg gataatactg atatattatg ctgatagtga cttcagctgt
     7261 aggaatattt tattgacaaa tgattttagc gtgatatgat agtaacaata aaaaatagac
     7321 cagggtaata ttccctaaaa ataaaagaaa agaggattat ctggatgtca aattgggaca
     7381 ctaaattttt gaaaaaaggc tacacttttg acgatgtctt gcttattccg gcagagagtc
     7441 atgttcttcc aaatgaagtt gatttaaaga caaagctcgc agacaatttg acattaaata
     7501 ttcctattat tacagcagct atggatacgg ttactggtag taaaatggct attgcaattg
     7561 cgcgtgcggg tggtttaggt gttattcata aaaacatgtc tattactgag caagcagaag
     7621 aagttcgcaa ggtaaaacgt tctgaaaatg gtgtcatcat tgatccattc ttcttaacac
     7681 ctgaacacaa ggtttcagaa gctgaagaat taatgcagcg ctaccgtatc agtggtgtgc
     7741 caattgttga aactttagca aaccgtaagt tggtcggtat cattactaat cgtgatatgc
     7801 gttttatttc tgattacaat gcaccaatct ctgaacatat gactagtgag cacctcgtta
     7861 cggctgctgt tggtacagat cttgaaactg cagaacgtat tcttcatgaa caccgtattg
     7921 aaaaacttcc tttggttgat aatagcggtc gtctgtctgg tttaatcact atcaaagata
     7981 ttgaaaaagt aatcgaattc ccacatgctg ctaaagatga atttggtcgt cttctggtcg
     8041 ctgctgcggt aggtgttacc tcagatactt ttgagcgtgc cgaggccttg tttgaagctg
     8101 gtgcagatgc gattgttatt gacacagctc atggtcattc ggctggtgtt cttagaaaaa
     8161 ttgctgaaat tcgtgctcat tttccaaatc gtactttgat tgctggaaat attgccactg
     8221 ctgaaggtgc gcgtgctctt tatgatgcag gtgttgatgt tgttaaagtg ggtattggtc
     8281 ccggatctat ctgtaccact cgtgtggttg caggtgtcgg tgttcctcaa gtgactgcta
     8341 tttacgatgc tgctgctgtt gctcgtgaat atggcaaaac gattattgct gatggtggta
     8401 tcaagtattc tggcgatatt gttaaggctc ttgcagccgg tggtaatgct gttatgcttg
     8461 gatcaatgtt tgcaggtact gatgaagctc caggtgaaac tgaaatctac caaggccgta
     8521 agttcaaaac ttatcgtgga atgggttcca ttgcagctat gaagaaagga tcaagcgatc
     8581 gttacttcca aggttccgtc aatgaagcta ataagttagt tccagaaggt attgaaggcc
     8641 gtgttgctta taaaggagct gcttcagata ttgtcttcca aatgttaggt ggtatccgct
     8701 caggtatggg ctatgttggc gcaggtgaca ttcaagaatt acatgaaaat gctcaatttg
     8761 ttgaaatgtc aggtgcaggc ttgattgaaa gtcatccaca tgatgtccaa atcacaaatg
     8821 aagcaccaaa ctattctgtg cactgataat ttaatttagt aactttaagg gctgacatat
     8881 tgtgccagct ctttttttat attaaaaaat gataaaatat aataacaatt ttcggataac
     8941 ttttattgtt ttttcttagt aaataagcta taattagtct ttacatgaaa ccattttatg
     9001 atagaattat tataatcttt aaagatttac tataaaggag ataacatttt ggaaggtatt
     9061 ttttatgcac ttattcccat gtttacatgg ggaagcattg gatttgttag taacaaaatt
     9121 ggaggaaaac cttcgcaaca aactctagga atgacttttg gggctttatt attttcatta
     9181 gctgtttggt taattgttag acctgagatg acactgcaac tttggatttt tgggatttta
     9241 ggcggtttta tctggtcaat tggtcaaact ggtcaatttc atgccatgca atacatggga
     9301 gtctccgttg ccaatccttt gtcaagtggc tctcaacttg ttctgggaag tttgattggt
     9361 gtcctcgttt ttcatgaatg gacaaaaccg atgcaatttg tagtaggaag tctagcacta
     9421 ttacttctga ttgttggttt ctacttttct agtaaacaag atgatgctaa tgcacaagtc
     9481 aatcaccttc ataatttttc aaaaggattt agggcgctca cttattcaac aattggttac
     9541 gtgatgtatg cagtcttatt taataacatc atgaaatttg atgttttatc tgttatttta
     9601 ccaatggcat ttggtatggt cctaggagct ataactttca tgtcatttaa aatttccatt
     9661 gaccagtatg tgattaaaaa tagcgttgtt ggattacttt ggggcattgg gaatattttt
     9721 atgcttttag ctgcatcaaa agcaggactt gctattgcct ttagtttctc acaattaggt
     9781 gctatcattt cgattgtcgg aggcatttta ttcctaggcg aaaccaaaac caaaaaagaa
     9841 atgcgttggg ttgtcacagg aattatttgt tttatcgtag gtgctatttt attaggtgtg
     9901 gtcaaatctt aatcgccaca taatatcaaa aaaaggctca gtttaactga accctttttt
     9961 gatgttatta gtgaatatca gaatcaattt gaacggttcc ttttgtcacg tgaaaaatac
    10021 ggataccttc tggcaactgt gacaggtgat ccaaacttgt tgttgtgata aaggtttgaa
    10081 cattttcttt aattactgtt tcaagtaact ttgtttgtcg tgtattgtct agctcgctca
    10141 tgacatcgtc tagtaaaaga atgggattat ctccagtcaa ggccttcatt aaactaactt
    10201 cagccatttt taaggaaaga ataaggctgc ggtgctgacc ttgactagca aagttagcat
    10261 tcatcccgtt aatgtaaaag gctaagtcat cgcgatgtgg gccaacgcta gtgtttttac
    10321 ggaagaaatc tttctgatga tttttttcaa gttggtgtaa aaattgttgg taaatattag
    10381 tttttttgtc aaagacaact gacgactgat aggagagaga gagactttct aaaccatttg
    10441 agatggcttg gtgatgagta tttgcttctt tctctaacgc attgataaaa tcaatacgat
    10501 gttccatgac acgggctcca tagctagcta attgctcatc tagaacagct agaaaagcgg
    10561 catcaatttg ttgggcactt tttaaatagc tattacgctg tttaagcacg tggttataat
    10621 gggataactc agataagtaa acaggcttaa tttgaccaag gtcaatgtcg ataaatttgc
    10681 gacgaaggct aggtgctccc ttaactagtt gcaaatcttc tggtgcaaaa agcacaacca
    10741 tcatcgttcc aatataatca gataattttg cttgctttag cgcattaatc tttgttacac
    10801 gtcctttatc tgatagattg atttctaaat caacagtacc actaatacgc tgaatcttac
    10861 cagtaagaga aacagttgag tgatcaaaat gaatcaattc tttatcagcc ctcgtgcgat
    10921 gacttctagt aagtgataga aaatagattg cctcgagaaa attggttttt ccttgagcat
    10981 tattaccgat aaagacattt aaacccgacg aaaatgaggc aagcaggtgg tcataattgc
    11041 gataatgttt taactctaac tctttaatcc acatactaga tacctgggaa gcggacaggt
    11101 gcagttggtt tacctttagt tttcttagtt gctcttagtg attttttggt agtagattga
    11161 cgatttttat gtttagaaga agtctttttg ttagcttggt tcatttgttt cactagagcc
    11221 gctacacgtg tcttttcagc tatttcttcg gcaaattgtt ctttttcttc ttgactaggc
    11281 tcaacaatag tgataattaa gtcttggtca ggtagagaaa tcttatcccc tactctgatt
    11341 tttttgcctc gacgtttctc atcttcacca ttaaatagaa cggttgtttc agcgagaaat
    11401 ctcttgatag cgccaccact ttggataatt cctaattctt taagaagggc ttgtagggtg
    11461 ataaattctg taaataattt gt
//
LOCUS       DAUCWO010000055        10952 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUCWO010000055 DAUCWO010000000
VERSION     DAUCWO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.055"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..704
                     /locus_tag="VGY20_001352"
     CDS             <1..704
                     /locus_tag="VGY20_001352"
                     /inference="COORDINATES: protein motif:HMM:NF013550.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4274200.1"
                     /translation="TGPEGPQGKTGPAGPAGPRGEQGLQGDRGEVGPRGEQGPAGPAG
                     KDGEKGDQGPMGPRGDRGEAGPAGKDGERGPVGPAGKDGQDGKDGLPGKDGQNGKDGL
                     PGKDGKDGQDGKDGLPGKDGQDGKDGQPGKPAPKTPEVPQKPDTAPHTPKTPQIPGQS
                     KDVTPAPQNPSNRGLNKPQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIM
                     TTAGVVAVAKRQENN"
     gene            874..1329
                     /gene="msrB"
                     /locus_tag="VGY20_001353"
     CDS             874..1329
                     /gene="msrB"
                     /locus_tag="VGY20_001353"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ4274201.1"
                     /translation="MKRSFPWKHPDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEK
                     GIYVDIVSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAG
                     SHLGHVFSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            1459..2478
                     /locus_tag="VGY20_001354"
     CDS             1459..2478
                     /locus_tag="VGY20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ4274202.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKCILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDAIHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADSFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            2685..3110
                     /locus_tag="VGY20_001355"
     CDS             2685..3110
                     /locus_tag="VGY20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4274203.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            3137..3628
                     /locus_tag="VGY20_001356"
     CDS             3137..3628
                     /locus_tag="VGY20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ4274204.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3645..4454
                     /locus_tag="VGY20_001357"
     CDS             3645..4454
                     /locus_tag="VGY20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ4274205.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4451..5278
                     /locus_tag="VGY20_001358"
     CDS             4451..5278
                     /locus_tag="VGY20_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4274206.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5414..7063
                     /locus_tag="VGY20_001359"
     CDS             5414..7063
                     /locus_tag="VGY20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4274207.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDELGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            7067..7855
                     /locus_tag="VGY20_001360"
     CDS             7067..7855
                     /locus_tag="VGY20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4274208.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7849..8895
                     /locus_tag="VGY20_001361"
     CDS             7849..8895
                     /locus_tag="VGY20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4274209.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMCIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            9010..10407
                     /locus_tag="VGY20_001362"
     CDS             9010..10407
                     /locus_tag="VGY20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ4274210.1"
                     /translation="MTYQTIYPYTNEVLHTFDNMTDQGVADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQVKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10509..>10952
                     /locus_tag="VGY20_001363"
     CDS             10509..>10952
                     /locus_tag="VGY20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ4274211.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPF"
BASE COUNT         3380 a         2017 c         2261 g         3294 t
ORIGIN      
        1 agaccggtcc tgaaggccca caaggcaaaa ccggcccggc aggtccagca ggtccacgtg
       61 gtgagcaagg cctgcaaggt gatcgcggtg aagttggacc aagaggtgaa caaggtcctg
      121 ctgggccagc tggaaaagat ggtgaaaaag gagaccaggg tccaatgggt ccacgtggtg
      181 accgcggcga agccggtccc gccggaaaag atggtgaacg tggtccagta ggtccagctg
      241 gcaaggacgg ccaagatggt aaagatggtc ttccaggcaa ggacggccaa aacggtaaag
      301 atggtctccc aggtaaagac ggcaaggacg gccaagatgg caaagatggt cttccaggca
      361 aggacggcca agatggcaag gacggtcaac caggtaaacc ggctcctaaa acaccagagg
      421 tccctcaaaa accagatacc gcaccacata ctccaaaaac ccctcagatc cctggccaat
      481 caaaagacgt gacacctgct cctcaaaacc cttctaatag aggtctaaac aaaccacaaa
      541 cacaaggtgg taatcagctc gcaaaaacac cagcagctca cgacacacac agacaattgc
      601 cagcaacagg cgaaacaacc aatccattct ttacagcagc tgctgtagct atcatgacga
      661 cagctggagt tgtagctgtt gcaaaacgtc aagaaaacaa ctaatcctct ttttgctacc
      721 taactgatgg aaagtaaaac tcagagagaa cctcaaggtt ctctcttttt ttgaatcaag
      781 atagtagctc taggtataac gtcttgtctg gggtgctgtg aacaaatcag ttactgctca
      841 cctctttttt tggtaaaata taagcactaa aacatcaaaa ggagttttcc atggaaacat
      901 ccagatgaat taaaacaacg cattggtgat ctttcttacg aggtcaccca gcatgcagca
      961 acagaatctc cctttacagg cgaatatgat aatttttttg agaagggtat ctatgtcgat
     1021 attgttagtg gtgaggtctt gttttcatca ctggataaat ttaattctgg ctgtggttgg
     1081 cctgcctttt caaaacccat tgaaaatcgt atggtcacaa accacgatga ctcttcttat
     1141 gggatgagac gagttgaggt taaaagccgt gaagcaggat cgcatttggg gcatgtcttt
     1201 agtgacggcc ccaaagaagc tggaggcttg cgttactgta tcaattcagc agctcttaaa
     1261 ttcattcctt atgagcagat ggaaaaagaa ggctatgccc agtggctcac attgtttgat
     1321 gagacataac agggtttcaa aatgactgaa aaagaagcgt cagtgattcc cataagcact
     1381 ggattggtga ggcgtacggg tcaattttac aagcctttgt aaaaggtcta taataaggaa
     1441 tgaaaaaagg aggttagtat gtcaactcac ttaagaaaac ttccaggcct gttactttgt
     1501 ctgcttttag ctcttccagc ctggtgttta gggcgcttgt ttcctattat tggagctcct
     1561 gtttttgcta ttcttttagg aatgttgtta gccttgtttt atgaacatcg tgacaagact
     1621 aaagagggaa ttagttttac atccaagtgt attttgcaaa cagcagtggt tttgcttggt
     1681 tttggattaa acctaaccca agttatggca gtaggcatgc agtctttacc cattatcatt
     1741 tcaactattg cgacagctct tttggtagct tatggctcac agaaatggct gcgcttagat
     1801 gtcaatacag ccaccttggt gggtgtaggg tcttccattt gtggggggtc tgctattgca
     1861 gcgacggctc ctgtcattaa ggcaaaggat gatgaggttg ctaaggcaat ttcagtcatt
     1921 tttctcttta atatgttagc agctttgcta tttccaagtt taggacaatt actgggctta
     1981 tctaatgaag gttttgctat ttttgctggg acagctgtta acgatacctc ttcagtaacc
     2041 gcgacagcaa cagcttggga tgccattcac cattccaata cactagatgg cgcaaccatt
     2101 gttaaattga ctcgcacctt ggctattctt ccaattactt taggcttatc cctttaccga
     2161 gcgaaaaaag agcacgacat cgttacagaa gaaaacttta gccttaggaa gtcattccct
     2221 cgtttcatcc tcttcttttt attagcttct ctcattacaa cgctgatgac cagtttggga
     2281 gtttctgccg atagtttcca ttccctaaaa accttatcaa aattctttat cgtgatggct
     2341 atggcagcga ttggtttaaa cacaaacttg gttaaattaa tcaagacggg cggtcaggct
     2401 atccttttag gagctatttg ctgggtagct atcacccttg tcagtttagc catgcaatta
     2461 agtttgggca tttggtaatc agacacgaga aatcagtttg actgatttct ttttttgtat
     2521 cttatttgtg tagaattgtc tacattttaa agagaatagt gcattttaaa aacgttttca
     2581 tttgataaaa taaacctatc gttaaaccag ttcagtaaag acaactggtt gagataagca
     2641 attaccgttt attcagcggt ttttgttaat agaggaggat aggaatgaaa cgaaaatttc
     2701 tcattgggag tcacggcaga ctagccagtg gtttgcaaag ctctattgac attttagcag
     2761 gtatgggaca ggcacttgaa accattgatg cttatgttga tgatagtgac tacactagcc
     2821 agattgatga ctttatcgct ggggtagcag cagatgagca gggccttatt tttactgatt
     2881 tgttaggcgg cagtgtgaat cagaaaatgg taaccgctgt aatgaacagt ggcaaagaca
     2941 atatcttttt aatcacaaac agtaacttag ccactttatt gtcgttggta tttttaaaac
     3001 caggcgaagc gcttactaaa gatgaaattg taaccgttat taatgaatcg caagttcagt
     3061 tagtggattt ggtccctgag acaaattcag aagatgactt ttttgattaa tatcttaata
     3121 tctaggagga agacaaatga ttacccaaat tcgtgttgac gaccgtttga ttcacgggca
     3181 agtggcagtt gtatggacca aagagttaaa tgcgccgctt ttagtagtgg ctaatgatga
     3241 agctgcaaaa aatgaaatca cacaaatgac gcttaaaatg gctgtgccaa acggcatgaa
     3301 actgcttatt cgttcagttg aagactcgat taagctcttt aatgatccac gcgccaaaga
     3361 caagcgtatc tttgtgattg tcaattctgt taaagatgct tgtgccattg ctaaagaagt
     3421 tccagactta gaagctgtta atgtggctaa tgtggggcgc tttgataagt cagacccagc
     3481 aagcaaagtc aaagtgacac caagtctttt acttaaccca gaagaaatgg cagccgcaaa
     3541 ggaattagtc agtttaccag aacttgatgt ctttaaccaa gtcttaccat caaataccaa
     3601 ggtccatttg agccaattag tcaactaaag agaggtaaaa aagtatgtta gtaccagcaa
     3661 caatggcagc tttagctgta ttgatttgtt ttgggggaaa ttatttaacc ggtcaaagca
     3721 tgatggaaag acccctagta gttggactgg tcacaggtct tcttttagga gatatgaagg
     3781 tcggtatctt aatgggggcc tctctagaag ctcttttcct aggaaatgtg aatattgggg
     3841 gtgttattgc agctgagcct gtaacagcga ctgccatggc aacaaccttt accattattt
     3901 cacatattga ccaaaaagca gccatgaccc tagctgttcc aattggaatg ttagcagcct
     3961 ttgtggttat gtttttgaaa aacgtcttca tgaatatctt tgctccaatg gttgataaag
     4021 ctgcagcagc taaccatcaa ggtaaactag tgatgctcca ctatggcact tggattatct
     4081 attacttaat cattgcctct atttctttca ttggtatttt agtaggtagt ggacctgtta
     4141 atgcctttgt ggaacacatt cctcaaaatc tcatgaatgg cttgagtgct gcgggaggcc
     4201 tccttccagc tgtcggtttt gccatgctga tgaaattatt gtggaccaac aaattagctg
     4261 ttttttacct attgggcttt gtcttaacag cttatttgaa attaccagcg gttgcagttg
     4321 ctgccctagg agcagttatt tgcgtcatta gttcccaacg tgatctagaa ttagacgcaa
     4381 ttactagagg tgcaattagt aaacaaacaa cctttgactc taaagaatca gaagaggagg
     4441 acttcttcgc atgacatcac aagataattt gactaaagaa gaccgtaaaa tgcttcgctc
     4501 agtcttttgg cgctcatgga cgatgaatgc cagtcgtact ggggcaacac aataccacgc
     4561 agtaggggtt atttacactt tactaccagt catcaatcgt ttttataaga ctgataagga
     4621 taaagcagag gcactggtcc gtcatacaac atggttcaat gccaccatgc acatcaataa
     4681 ctttatcatg ggtcttgtgg catcgatgga aaagaaaaac agtgaagatc ctgattttga
     4741 tgctagtgct attacggcgg taaaagcatc tctaatggga cctatttccg gcgttggaga
     4801 ttccttcttc tggggaattt taagggttat tgcggcaggt atcgggattt ccttggcaag
     4861 tgcaggctca gccatgggag ctgttgtctt cttactcttg tacaatattc ctgcttttat
     4921 tattcactac tatagccttt atggtggtta ttctgtaggt gcaggattta tcaaaaaact
     4981 ttacgaatct ggtggtataa aaatcgtcac taaaacctca agtatgcttg ggttaatgat
     5041 ggtaggctca atgacggctt caaatgtaaa atttaaaacg attcttacag ttgctgcgaa
     5101 aggtgccaaa gaagcagctt ctattcaaga ttaccttgac caattgttta ttggtattgt
     5161 accattaatg gtaacactag cagctttttg gcttctacga aagaaagtga atatcaactg
     5221 gattatgttt ggtatcatgt tccttggcat tatccttgga cttttaggta tttgctaata
     5281 atctcctagc aataaaggta agtaagacaa gttttcttgt tttgcttacc tttttccttt
     5341 taagatgatg agacttgctt tgatgaaaaa cgtagtagca ttaagaaatt tgttataatg
     5401 aaaggagctt aagatgcgag gtgaacaggt ggaagaacat tttaaaaaac aattacaaga
     5461 tgatatttct agacattttt cgtaccaatc gttaatgtta tcattgttgc taattggtct
     5521 ttttattatt ttttcattag cgccacaaca attaggcctc tatcgtgata tcaatgccac
     5581 tgcaactcgt tatcaccgtt tgattagcaa acaagaagcc ttgctggatg aattagggaa
     5641 aaatagctta ctaccttttt taaataaaaa cctcagcact gctgatttaa gcaagcacta
     5701 ttttcatttg cgccatagca gccaaacgtc accagaactt ttactatttt cacctagtca
     5761 aggcctttta tttgctagta acccacattt aggaaatgtt tttagtaaat ctgtttatat
     5821 tcaagaagtc ttgagagcag ctcatcctcc aaaaaccttg tttaaagttg ctatggatag
     5881 tgaagatggt cactacttga tgatcattaa gccaatgatt gatcaaaacc aactaaaggg
     5941 atatgctttt ttagtgatga gtggcaaaga ttttcttcat cctacaaaaa cattgacgtc
     6001 agagttggtc attgctgata agctggacaa cacgtttacg ttttccaatc gtgagtttat
     6061 agcatctagt ctagataaga tcaatagcca gtatttacac cattattttg tttttcaaga
     6121 taatcgagcc tttatcacaa gaaaggttgc cttacaagga ggtctttggc tttacatgta
     6181 taggcccttg attccaatgg tatcggtgat gttattttca cttatttcat cagctgttat
     6241 ctttgtgatc ttgcaacgta aatcaagtgg tttagctaac cgaattgcag ctaaaaattc
     6301 aagagcaatc aatcaaatgg ttagagatat gagtgcgatc tctcggcaag aaaaaagacg
     6361 tattgacctt gagagtcaag atgaatttca atatttatct gatcaaatca atcaaatggt
     6421 agagcgattg caacagttac atgataaaac gttagatttg gaaactcaaa aattattatt
     6481 tgaaaaacgg atgttagaag ctcaattcaa tccgcatttc ctctacaata cgctagaaac
     6541 cattttgatt acgagccatt atgattctgc cctaacagaa aaaattgtta tccaattgac
     6601 aaaattgttg cggtatagtc tcacggattc tagcaaacct gtcttgctca aagatgattt
     6661 aagtgtcatc gagtcttatt tagtgattaa tcaggtgcgg tttgaagaat tgcaatatag
     6721 cattaaccta tccccagacc ttgatagtct tgcagtgcct aaattgttct tgttaccttt
     6781 gatagaaaat gccatcaaat atggcttgaa agaacgacac gatgtcaaga ttaacattgc
     6841 ttgttattat caagatgacc acattatttt ttctgtgcga gataatggtt cgggaattga
     6901 tgctcatcac caaaaagtga ttcgagagca attggaagct ggagagtcac accatgggtt
     6961 aattaactct tatcgtcggc ttaagtatca tttttcagaa gtatccttgg tttttgatca
     7021 aggtgataaa cagtttaatg ttagctacca tgttaaggag tagtctatgt acaaattagt
     7081 cattattgaa gatgaacata ttattaggaa atggttgcga tacgctattg attataaagc
     7141 tttagatatt ttggtcattg gcgaggctaa agatggcaaa gaaggagctg ttttaatcaa
     7201 agagagtcaa ccagatatcg ttttaaccga tattaacatg cccatcatga cagcttttga
     7261 catgtttgaa gcaacaaaag atcaaactta cgccaaaatt attttatctg gctatgccga
     7321 ttttccaaat gcaaggtctg ccatacacta cggtgtactt gaatttttaa caaaaccgat
     7381 tgaaaaagca gctctttggg aatgtcttca aaccattatt gctaaaatag aaaagcaaaa
     7441 ggggtctaat caaaaaacag atgcttgcgt ctatattccc ttaccccaga tgactgatca
     7501 gattcctgaa gtagtcaaag atattttgga gtgggtacat gctcattttc aagacaagat
     7561 cagcacgagc cgcctagctc atgacttagg gtatagtgag agttatatct atcaaaatat
     7621 caaaaagcat cttcagatgc ctttaagtga ttacattaat caatatcgga ttaatcaggc
     7681 aattcaattg atgcaacaag aaccagattt aatggtatat gaaatagcac aagctgtagg
     7741 tatttatgac tatcgctatt ttgatagggt ttttaaaaag tatctaggac aaactgtaaa
     7801 ggcttttaaa gaagagcatt ttatgaaaga taccgatagg agtaagccat gttaaaactc
     7861 aaacgcaaat ggctattaag ctttttgtta gtaataatta ttttagcaat cgttaacgtt
     7921 gcaatgtgta tattctcatc gtcaaaaaaa gatagtgcta aagaattagt tatcttgact
     7981 cctaatagcc aaactatttt aacagggact attccagcct ttgaggaaaa gtatggggtt
     8041 aaagtaagat taatccaagg tgggacgggc caacttattg atcaattagg tcgaaaagat
     8101 aagccattaa acgctgatat tttctttggt ggcaattaca ctcaatttga aagccataaa
     8161 gatttatttg aatcctatgt ttctccgcag gtttctactg tcatttcaga ttaccaattg
     8221 cctagtcatc gtgcaacccc atatacgatc aatggcagtg tactgattgt taataacgaa
     8281 ttagcaagag gacttcatat taccagttat gaggatttgc tacaaccagc tttaaaaggc
     8341 aaaattgctt ttgctgaccc aaatagctca tctagcgctt tttcacaatt aacaaacatc
     8401 ttactggcta aaggaggcta tacgagctca gaagcttggg agtatgtgaa acgtttatta
     8461 ctgtctatga attctatcag agcaacaagt tcttcagatg tttacgaatc tgttgcagaa
     8521 ggtaaaatga ttgttggttt gacctatgaa gatccttgca tcaatctgca aaaaagcggt
     8581 gcaaacgttt cgattgttta tcctgatgag gggacagtat ttgttccgtc atcagtcgct
     8641 attatcaaaa aagcaaaggc aatgaaagaa gctaagcaat ttattaattt tattttatca
     8701 tcagatgtac aaaatgcctt tgggcagtca actagtaata gacctatccg acgagatgct
     8761 caaacaagtc atgaaatgaa accattagat gaaattatta ctttaaaaga agattacgga
     8821 tatgttacta gacataagaa aaaaatattg aatacctata atcgactaca tgaagcatta
     8881 gaaagaggaa agtagactct aggcttgttg ttgctatact agtagtgtta caaaatagcg
     8941 tagtcatttt ttgaaatcga tttcataatc tggtagacta aggttatcac gaaaaggagg
     9001 ctatatgtga tgacttatca aactatttac ccttatacaa atgaagtcct tcatacattt
     9061 gataatatga ccgaccaagg tgtggcagat gtccttgaaa gagctcacct tctctataaa
     9121 aaatggcgca aggaagatca tttagaagaa cgtaaagccc agcttcatca agtggctaat
     9181 attcttcgcc gtgaccgcga taaatatgct gaaatcatga ccaaagacat gggaaaactg
     9241 ttcacagaag cccaaggtga agtagacctt tgtgccgata ttgctgatta ctacgctgat
     9301 aaggcagatg aatttttgat gtctacgcca ctagaaacag attcaggtca agcttactat
     9361 ttgaaacaat caactggtgt tatcctagct gttgagcctt ggaatttccc ttactatcaa
     9421 atcatgcggg tttttgcgcc aaactttatt gttgggaacc caatggtttt aaaacacgcg
     9481 tccatttgcc cacgctctgc ccaatctttc gaagaattag ttcttgaagc tggtgctgaa
     9541 gcaggtagta ttaccaattt gtttatttct tatgaccaag tatcacaagt gattgctgat
     9601 aaacgtgtgg ttggtgtctg cttgacaggt tccgaacgtg gaggagctag cattgcagaa
     9661 gaagctggta aaaacttgaa aaaaacaacc cttgaattgg gtggagatga tgcctttatt
     9721 atccttgatg acgcggattg ggatcaatta gagaaggtgc tttacttctc tcgtctctac
     9781 aatgcaggac aagtatgtac ctcatcgaaa cgttttatcg tgcttgacaa agactatgac
     9841 cgtttcaaag aattattaac aaaagtcttc aaaacagcta aatggggtga cccaatggat
     9901 cccgaaacga cgttggcacc attatcatca gctcaagtta aagcagatgt tcttgaccaa
     9961 attaagttgg ccttggatca tggtgcggag ttggtttatg gtggtgaagc gattgatcat
    10021 ccaggtcatt ttgtcatgcc aaccattatt gctggcctca caaaagacaa ccctatttac
    10081 tatcaagaaa tctttggccc agtcggtgaa atctacaaag tgtcttctga agaagaagcg
    10141 attgaagtgg cgaatgattc taactatggc cttggaggta ctatctttag tagcaatcaa
    10201 gaacatgcga aagccgtagc tgctaaaatt gaaactggta tgtcttttat caactcaggt
    10261 tggacaagtc ttccagaact cccatttggt gggattaaac attcaggcta tggtcgtgaa
    10321 ttgagtgagc ttggctttac atcttttgtg aatgaacacc ttatctatat tccgaataaa
    10381 acgaataata gcaatactaa agtttgatct agattgttaa aggagatctg ctcgccttaa
    10441 taaacaatca aggcgttaca ctcttctttt ttaaagtcaa aattttaaaa atttgataaa
    10501 ctagtaacat gaacgaactg attaaacata aactagaact cttaccagac agccctggct
    10561 gttacttaca taaagataaa gaaggaacta ttatctatgt cggtaaggca aagaatttga
    10621 aaaaacgcgt ccggtcctat tttcgtggga gccatgatac caaaacagag ttactagtat
    10681 ctgagattgt agatttcgaa tatattgtaa cagagtcaga taccgaagcg cttttgttgg
    10741 aaattaactt gattcaaaaa aatatgccta agtacaacat caagttaaaa gatgataaat
    10801 cttatccctt tctcaaaatc acaaatgagt cttttcctcg gttagtgatt acaagatata
    10861 ttaagaaaaa tgatggattg tactttgggc cctacccaga ttcttacact gctaatgaag
    10921 ttaaaaagtt attagatagg atttttccat tt
//
LOCUS       DAUCWO010000056        10524 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUCWO010000056 DAUCWO010000000
VERSION     DAUCWO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.056"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(50..787)
                     /locus_tag="VGY20_001364"
     CDS             complement(50..787)
                     /locus_tag="VGY20_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274212.1"
                     /translation="MNQLLLKNVCKRYPNQPNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISVFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNIDSSKIDDLINSYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKKDCGESFYDVISQALSDRGE"
     gene            complement(784..1317)
                     /locus_tag="VGY20_001365"
     CDS             complement(784..1317)
                     /locus_tag="VGY20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274213.1"
                     /translation="MDKKDFRDISAFVSQDSPIFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIISMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRVLVTHRLNTVEKADKILIMDNGKIIDYGSHHCLYKNNEYY
                     RDLYDSYMNNYQEEGIK"
     gene            complement(1319..2944)
                     /locus_tag="VGY20_001366"
     CDS             complement(1319..2944)
                     /locus_tag="VGY20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEQ4274214.1"
                     /translation="MIKRDELKLEYQNHQFYEYVNSIFDYDILDTSIRETSEVLRKKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQYIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKTDYFQEDGYNIPTLNGKVVEPLDYANEVIADFRECYTFLMSQRAKV
                     KKILEDFPKLKTRAIFRNTSDYGKFLQASTNPKYLFSEKKRENLFSILHESKHIEQFI
                     VVSEIKDLMNGDIPYFSMDTSGNVYNSLGTVIGNLGETTSLFDNIATLNDERMKFTCE
                     LLGIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            complement(3028..3183)
                     /locus_tag="VGY20_001367"
     CDS             complement(3028..3183)
                     /locus_tag="VGY20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEQ4274215.1"
                     /translation="MSFMENSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            complement(3688..5094)
                     /gene="lacG"
                     /locus_tag="VGY20_001368"
     CDS             complement(3688..5094)
                     /gene="lacG"
                     /locus_tag="VGY20_001368"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ4274216.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNLDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFTALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYIDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(5167..6864)
                     /locus_tag="VGY20_001369"
     CDS             complement(5167..6864)
                     /locus_tag="VGY20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ4274217.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLSSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHSTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFALFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDVLKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEE"
     gene            complement(6864..7181)
                     /locus_tag="VGY20_001370"
     CDS             complement(6864..7181)
                     /locus_tag="VGY20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4274218.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(7205..8188)
                     /gene="lacD"
                     /locus_tag="VGY20_001371"
     CDS             complement(7205..8188)
                     /gene="lacD"
                     /locus_tag="VGY20_001371"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ4274219.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEVGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDINEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKISDNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(8192..9121)
                     /locus_tag="VGY20_001372"
     CDS             complement(8192..9121)
                     /locus_tag="VGY20_001372"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ4274220.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCVGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHQFKYLLNDVDVVTMSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(9167..9682)
                     /gene="lacB"
                     /locus_tag="VGY20_001373"
     CDS             complement(9167..9682)
                     /gene="lacB"
                     /locus_tag="VGY20_001373"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ4274221.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHKVIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRSALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(9717..10145)
                     /gene="lacA"
                     /locus_tag="VGY20_001374"
     CDS             complement(9717..10145)
                     /gene="lacA"
                     /locus_tag="VGY20_001374"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ4274222.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKHEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
BASE COUNT         3273 a         2081 c         1578 g         3592 t
ORIGIN      
        1 taacgaaaaa ttttttttaa tgttagattt tgtaattgac caaatcatat cactctcctc
       61 tatcggataa agcttgacta attacatcgt aaaacgactc tccacaatct tttttatcaa
      121 tctccatgtg aagtttacca tctttgagaa gtacaactct atccgcataa gaagctgata
      181 aaacgtcatg agtaaccatt aaaatagtta ccttttctgt tttattgatt tcactcaaaa
      241 tcatcattgt ttcctcagaa gatttcaagt caagtgctcc agttggttcg tcagcaaaaa
      301 taatctctgg ttctttaatg attgctctga taatactgac cctctgtctt tgacctccag
      361 aaagttgata cggatatttc ttcaactgtt catataaatt aaatcttttt gcatagctat
      421 ttatcaaatc atcaatcttt gaagaatcaa tattttttag agacaaagca acagaaatat
      481 tctccctaat ggttagacta tctaacagca tataatcttg aaaaacaaaa ccaatatcat
      541 ttttcctaaa actagttgcc tcgttatcag aaaatacact aatttctttg tctgcacaat
      601 aaatactccc actatcaatt ttatcaataa ttgaagtaac atttaaaagt gttgtttttc
      661 cactaccact acgtcccatg acagctacaa attctccttt tctaacagtc aaattgatat
      721 ggtctaaagc ataattcggt tggtttggat accttttgca aacatttttc aatagtaatt
      781 gattcatttt atcccttcct cctgatagtt gttcatgtac gaatcatata aatcacgata
      841 gtactcatta tttttatata aacaatgatg actaccatag tcaataattt tgccattatc
      901 cattattaaa atcttatcag ctttttcaac tgtattaagt ctatgggtaa ccaaaaccct
      961 cgtacaatgt aaagcctcaa catttttttg gattattctt tcagttttta catctaaagc
     1021 tgatgtcggt tcgtcaagaa ctaagattct aggggtagtt acaagttctc ttgctaaaga
     1081 aattcgttgt ttttgccccc cagatagtga tggattatct cgaaaaagtg gagtatgaaa
     1141 cttcattggc atacttatga tatcgtcata tattgatacc cttttacaag tttcaataac
     1201 ctgttctcct gaaacagatt ctctccctag cgaaatatta tacatcacat ccccatcgaa
     1261 tatgggagaa tcttgagata caaaagccga tatatcccta aaatcttttt tatcaatttt
     1321 agagaaggta atgttctgag gagattgata aaaactgata agatttgccc ttttccctct
     1381 cccaatgctt aatcggtttt ttcaagacta ttccaagcaa ttcgcaagtg aacttcattc
     1441 tttcatcatt cagggtagct atattatcaa ataatgaggt tgtctctcct agatttccaa
     1501 ttactgttcc taaagaatta tatacatttc cactggtatc cattgaaaag taaggaatat
     1561 caccattcat caaatcttta atttcactaa caacaataaa ttgttcaata tgttttgact
     1621 catgtagtat cgaaaataaa ttctccctct ttttttctga gaaaagatac tttggattcg
     1681 tagaggcttg taaaaatttt ccatagtcgg aagtatttct gaatatggct ctagttttca
     1741 attttgggaa atcttctaaa atttttttaa cctttgcacg ttgcgacatg agaaatgtat
     1801 agcattctct aaaatctgca attacctcat tagcatagtc gagaggttcc accactttcc
     1861 catttaatgt cggaatatta taaccatctt cctgaaaata gtcagttttc actaacttca
     1921 tatctccacg atttttattt atcagttcat attttccttt ttttcttttt ccaccttttc
     1981 cacttattcc agaaacgtta ggagcgctgt ttttctcaaa ttgtacagga aataaaccgt
     2041 ttgaaagtga tgtctcataa attctctgat aggtgtctac agtaatccct tcaaaattct
     2101 gattttgtac attcaacttt ctttgaaaaa aggtttcaac gtcaattaaa tataaatctc
     2161 cgcctgaaac aatcacattt tccatatgta agtccgtaat agaaaatatt tcagaaaatg
     2221 ctgctaaaac accatactta aagtaaattt tcgctacttc atctttattg gaacttgtat
     2281 aagctacacc aagttgccaa caataatctg ctttaactaa gcttttagga agactgaatg
     2341 aaggtatatt actagatttc aataaactag taagttctaa caagatactg tgtgaatgat
     2401 aacttttagg cttgtagtaa acagatttct ctttaaacgc aattttaaca actgtttgct
     2461 ttttagagtg actgtcacca agtgaaaagg caacattgct taggggctca ctaatgttaa
     2521 agcacgacct tatctcagat aagtcttcta aaaatctatc aaataataaa gaatatgatt
     2581 cctctatcag tcctacttct ttattaattt gttttagcaa atacggatac ctgtcaaaaa
     2641 aatgatttat tccgttttct tctcttatct gctgtatata ctgctcatat gcctctgact
     2701 tattttcaat ttccttggaa aaatgattta tatctaaaac tagtgttttc atcgaaagga
     2761 acatgatcgt ttcaaataac tgattttcaa actcactata aaataactta tgtgtctttt
     2821 tccttaacac ttcactggtt tctctaatag aagtatctaa tatgtcatag tcaaaaattg
     2881 agttaacgta ttcgtaaaac tgatggttct gatattctaa ctttaattca tctcttttta
     2941 tcattttttt cctaccacta cttaaaaatc ctgcctagac tgagtctaaa caggaaattt
     3001 ttagtctgct tgtccagatt gttttaatta acaacaaaca aatactgagt tcggacagtc
     3061 atcagtaata gttgcaaacc aaccggaacc ttttttacca ccagctactt ccataagttc
     3121 tttttcagaa acttcttcga tagcattagt caaaatatcc tttgaatttt ccataaaact
     3181 cattctcctt tccatcagta tgtagtgact attttctaca cttttattgt aaataatacg
     3241 tcttcatttt caatgagtaa aaagtgcaaa aatgactttt tcatattttt tagtaagatt
     3301 tttgagatgt cacataagag gtcactttat aacaaatagc cctctgatat aaaaaaaaac
     3361 aaaactcgtt ctttacagct attttacaat ctaatcgctt ctcgttataa catcaatctc
     3421 taatcagaaa atggttactt tacttattta atcaggtcag gagtttgaat gacagaagtc
     3481 aaatatctga aaaaggatgt taaataaaac tttcaaaacc cattttcatc aactaaaaac
     3541 acttataacc cgatcttaca actaatggat taccatcaac agcaaataat actatgcttg
     3601 aaaaatgtca acatcatcta ctttttgtga ctataattca gtaaacgaac atgatgtgta
     3661 aaaatgtatt aaaaaagtat ctcaaaatca ttcaatcact tgagtttctg ctactttttt
     3721 ataccagtag gcactcttct taggataacg ttcttgagtc tcaaaatcga tatagaagag
     3781 accgtaacgt ttttcatagc catttgacca tgaaaagaca tccatcagtg accacataaa
     3841 gtatccttta acatttgcac catcagaaat agcatcagaa ataacttcta agtgtttttt
     3901 cacataatcg atacgtccat catcatagac agtattatct acaaactcat ctttgtaacc
     3961 aagaccattt tcagtgatat aaattttctt gtagttagga taatcagctt taacacgcat
     4021 gatttggtca tatagtcctt gtgggaagat aatccagtcc cagtccgttt ttggaacatc
     4081 aacgggtgct tttcttcgac caacaccctt gatttggtat ttagagctgc ctttttcccc
     4141 tttaccatta tgaatgattt cagtctcacc atcaaaagct tgcatccaat cactcatata
     4201 atagttaata ccaaggaagt cattcaaatc ttttgcggcg tctagtgcag taaaatcttc
     4261 ttcacgaaga tcaagttcac caccattcac ctcaaggata tggttaacac cttccattgt
     4321 tttatctgaa tacttaccta gataagttgc atcaagaata aatttattat ggatgatatc
     4381 ttccagttca gctgctctaa catcatcaag attgttagcg tcaaatggat acttagttgg
     4441 aagagcatgg acaacgccga tttcacctga ataaccacta tctttaaaaa gtttgactgc
     4501 acgagcatga gagaccatca tgttatggtg tgattggaaa actttagcaa gatcatattg
     4561 gatacctgga gggaatttac caactaagta ttgaccatcg ccaattggtc caatttcgtt
     4621 aaatgttgtc caatagttaa cttctgagaa ttctttaaaa caaaattctg cataatttac
     4681 aaaatgttca atgttctcac gattgaggaa atcaccatcc gagtggagag cttctggtgt
     4741 atcaaaatgg tgaagtgtaa caaaaggctc aacatgacgc ttatgacact ctgcaaaaag
     4801 attgtggtag tattctactc ctttagggtt aacttctcct tttcctgttg gaaaaatacg
     4861 agaccaggca atagagatac ggatcccatt aacaccaaat tcttcactaa gtttcaaatc
     4921 gacagggtaa cgattataaa aatcacttgc tggctcagct gtgtaccaat agttgtcttc
     4981 taagtattta tcccaagcta ctggtccttt gccatctgta tgggtagcac cttcagcttg
     5041 ataagcagct gtagcaccac caaaaataaa atctttaggt aatgtttttg tcatgatata
     5101 cctcttctaa attttcattt cactataaag gaagattgag tacccccaac cttccctagt
     5161 gaaatattat tcttccatct gttctttaac gaagtccaat gcaccttgac catcgcgagt
     5221 caatttgatg tattgagccc cttctgtttt agctaattta atatcaagtt tatcagtttc
     5281 cactttcatg tcttcatagt tagaagctac ttgaggtgca aggataacaa gttgatattg
     5341 tggtaaaatc tcacggtgag caccataact tcctgctgct gcagtgacag gtgcaccata
     5401 ttctttagct gctttattca aagcgtttgc aagcaaacca cttgtaccac caccggcaca
     5461 aagaacgaga acattagttt gatcagtgat ttctttggtt tctttagctg ctgatttttc
     5521 aagaatagca tcagcttttt tagtatcaaa gtttgctgct actttttctt tgaggacatc
     5581 tgatgaattc gtacctgctg cttcttcagc aagaatttgc tcatcatata ctttcaagaa
     5641 tgggtagtag atgatgacat caactacgat taacaaagct gccaaaatga atgctagcac
     5701 ttggaagttt gtcccaagaa taagacctaa tggagctggt gttgtccaag gtaagtttgc
     5761 agtgaaactg ttcattccaa gtgtgtcaat aaagaatttg aaaatccata cattgacaat
     5821 tggtgctagg ataaatggga taaagaaaat tgggttcaaa acaagtggag caccgaacaa
     5881 gataggttcg ttaacaccaa agaatgttgg aacaactgat gcgcgtccaa tggctttgtt
     5941 acgttttgat tttgttaacc acatgaacat gaatggaacg acaagtgttg caccagtacc
     6001 acccatggta acgatgaaca tttgagtccc tgaagtcaag atcttatcag catgttcacc
     6061 agcttgaagt aattgaaggt tggtttcagc atttgcgtaa gtaattgctg caatggctgg
     6121 ttcaacaata gatggtccat ggataccaac aaaccagaag agagcaaatg caccgaagat
     6181 aatagtgatt ccaacataac catctgctgc ttggaacaat ggtgcaagaa gtttaccaat
     6241 tgactcggca acgttaacgc ctaaagtact atgaacaatc aattcaatca cataaagcag
     6301 tacaactgat aacgtaaatg gaatcaaatc tttaaaaact tgagagatgt ttggtggcac
     6361 ctcttctggc atgcgaatag tgacattgtt ttttacacag actttataga catttacggt
     6421 aataaaagct gcgatgaagg ccgttaacaa accttttgtt cccataaatc cagttaagaa
     6481 accaccgtct tttgctggat ttgctgccat tagtagaaaa cctacaatag cagcaagcat
     6541 ggttgaaata aagttgattt ggttagtgct agacaatgag cggttcattg aatcggttaa
     6601 tgctttggct gtcgtaccag ccacaaacaa acctaaaata cccattgagt agttgtatgg
     6661 cgtcatcaag agattctcaa tatccttact ccaatggaag ccccatgcat ttggaacata
     6721 ggcaatcaag ataaagatac ttgaaaacaa gataacaggc ataccagcga taaaaccatc
     6781 acgaatagcg cgaagataga tgtttcgaga aattttctcg aagaaaggtt tccctttctc
     6841 aatctgagca attaatccat tcattatttt gctcctctct tataaagttc aatcatgtgt
     6901 ttcattaaat cttgtaataa aatggtcgtc attaaatgat cttgaccatg cattaaagtg
     6961 acactataag ctaagtcatc tccagctgct tctttttgta gtagacttgt ctgagcattg
     7021 tgagcatcat tcaagcagtc actacctgca gcgattaatt cttctgcttt agcataatcc
     7081 ccttcttgcg ctgctttaag cgcttccaaa aattttgaac gggcatcacc agcataggct
     7141 acgatttcaa aacctaaaag agtagtttct tctctattca ttgtagtttc ctccgaaaat
     7201 aatgttacat tttttcagtc caaggacttg cagttttatc tagcactttg tttaattcgt
     7261 caatattttt gaaaccttct gtacgcaacc attcacgagc tgcttgtggt ccttcttcga
     7321 tatagacttt tacagatcct gcccaggtag cacgtccaca taagacaccg ttgaatttag
     7381 cacctgattc tgctgcaaac accaaagtat cttggaacaa tttggcagaa acacctgcac
     7441 tcaagtaaat atatggtaaa tcagtagaag cttcttggtc acggaaagct tgagctgctt
     7501 cttcttttgt aaagagaacc tcaccatctg caaagctttc tacaaatttc atatttactg
     7561 gaacttcgac tttcaagacg tcaacaccaa agcgttcttc tgagaagact ttcattgctt
     7621 cattaacttt atgagcttta acttttgcaa actcagggct agcattgtca gagattttct
     7681 catcataagt tagaatttct aagtagaatg gaatatcttc tgcacggcat tcagaaccaa
     7741 tacgttcaat gtaagctttt ttctgctcgt tgatatcttg atctccatca atatcatagt
     7801 aaagaaggaa tttaactgct tctgctccag cttctttaat acgttttgca gaccaaacat
     7861 ctaagcaatc aggtaagcga ctagttgttg ttgcatcgta tcctgttttc tcatatgcca
     7921 acaacaagcc tacttcttct gaacgaacac gacttgctgg caagccatat tcaggatcaa
     7981 gtaaaattga tgatgcaaat ggtgttagtt cttcagaaac taaactttta agctcttcaa
     8041 tttgttctac tgttggctct tttgtttgat gttgagccat catacgtttg agagctccac
     8101 gttggtcaaa agctaaagca gaaataacac catctatact tagtttttcc atgcttttac
     8161 gtttgttttc agttaaagta attgtcattt tttatacctc tttgacttta atagattgat
     8221 ataacttatc gtaatttgcc atgttaacat gcccagttgt tttttcttgc gcatttaaca
     8281 tccctaagac atttgctttt gttaataaag ctcgatcatc ttcatcattc gctaaacctg
     8341 aagcaatacc tgccactgta gaatcccctg aacctacagc gcttacaatt ttgattttag
     8401 gaatgtcaac gttataaaat gtatccttgt gctttgcaaa tacaccatca gcaccaagag
     8461 aaacgattat ccattcaatt ccttcaaata gttcatcttg taagacttct tttaaggctt
     8521 caaaatcttt tgtcatttct ttacctaaaa gttgagaaag ttcttcaaga ttaggcttga
     8581 taactgttgg tttactatca ccttttagta ctgcttctag agcatttcca gaacaatcca
     8641 gaaccgtttt cttaccatta agattggcaa tcttgattaa cttctgatag taatcatctg
     8701 gcataccagc aggcaaactg cctgacattg tcactacgtc aacatcattc agtaaatact
     8761 taaattgatg agtaaatcca tcaatttcat ctacacttaa cagcggtcct ttttctaaaa
     8821 tctccgtttg attaccttcg tgtaaaatgg ctatacaagt tctagtatca cctgaaattt
     8881 tatagaagcg acttaaaatc gaatcaggaa gatgattaat gataaaatca ccgctttctc
     8941 cacctacaca acctgtagct ttgactgtct caccaaagtc atttagaact cgggaaacat
     9001 tcaagccttt accaccgggt gtctttgtaa cgtctacaac tcgattgaca gtatcgcatt
     9061 ttagctcatc caaagggtag gagacgtcaa tagctggatt taaagtaact gttaaaatca
     9121 caatagctcc tttttctata tatccttatc ataattcttt caaatattag tcgtgatatt
     9181 caccacggtc ccatttttct agaaattcat caaagaagtg tgggttgttt tgatcttggc
     9241 tagcatgttg caagctatcc attttttcaa tccatttttt attttcttca gttggtttgt
     9301 attccgcggc taagaaagca tcgataatat cattcatcaa taaaccaccg atgatttttc
     9361 caccaaaacc aataacgttt gcgtttagct cttcttttga gtagatagca gatgtcatat
     9421 ctctaacgag tgctgaacga attccaggaa ctttgtttac ggcatttgta ataccaacac
     9481 cagtaccaca gataacaaca cctagttctg cttcaccact tgctactgct tcaccaactt
     9541 ttttaccaaa aattggatag tgtgtgcgaa cattatcata cgtaccacaa tcaataactt
     9601 tatgaccttg tgattttagg taatccacaa cagcgatttt ttcataagta acaatatggt
     9661 cacaacctac agcaattttc atttttgttc tccttgtttt aacctatttc aatcaattag
     9721 cacattttat taagcatgtc gacacgaact tgatgacgtc caccatcata tttaccttcg
     9781 acaaagcctt tgataatgtt ctttgcaatt cctggagcac taatttcaga accaagcgtg
     9841 atgattcgag cattgttgtg tcctcttgtc atgtatgctg aacgctcatc tgatacttca
     9901 gctgccacca ttcctttgat tttagtagct accatgaaag aaccaacacc atatgcatca
     9961 atgacaatac ctaagttatc ttcggatgca ttaacttctt ttgcaacggc taaagttgta
    10021 tctacaaagt cacgtacttc gttttcacta acatcaacga cttcgtgttt accttcttta
    10081 aggtaatctt tgataacttc tttaagttct tgacctgctt tgtcagcacc gataataatt
    10141 gccattttat ttctcctatg atttagaata agtaaatgtg gaaaacctac gtagatagtt
    10201 aattaacgat ttacaacatc ttttgaaata acgctttcat tatttctatg acctaatcat
    10261 acacccacca aacataaaag tcaacatttt tttgtttatt tttataaaaa ataaacaaaa
    10321 tatgtttatt tttttcattt ttacgttatt atcctaatgt ttcttgtgtt tctttttcat
    10381 ttcataaatc cctttgacag ccactcgtaa cctatcatat attacgagac ccttatctct
    10441 aattattgta aacgttttat ttaaaacatt ttctattttg ttaatttttg ttttttccaa
    10501 tgtttatgtt tgttttgttt gatt
//
LOCUS       DAUCWO010000057        10427 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUCWO010000057 DAUCWO010000000
VERSION     DAUCWO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.057"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(414..1859)
                     /locus_tag="VGY20_001375"
     CDS             complement(414..1859)
                     /locus_tag="VGY20_001375"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ4274223.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDKLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYISEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(2041..2751)
                     /locus_tag="VGY20_001376"
     CDS             complement(2041..2751)
                     /locus_tag="VGY20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ4274224.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2882..3379)
                     /locus_tag="VGY20_001377"
     CDS             complement(2882..3379)
                     /locus_tag="VGY20_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ4274225.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVDTGKM
                     TQVME"
     gene            complement(3568..4929)
                     /gene="radA"
                     /locus_tag="VGY20_001378"
     CDS             complement(3568..4929)
                     /gene="radA"
                     /locus_tag="VGY20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ4274226.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(5010..5456)
                     /locus_tag="VGY20_001379"
     CDS             complement(5010..5456)
                     /locus_tag="VGY20_001379"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ4274227.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5566..6333)
                     /locus_tag="VGY20_001380"
     CDS             complement(5566..6333)
                     /locus_tag="VGY20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ4274228.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6451..8235)
                     /locus_tag="VGY20_001381"
     CDS             complement(6451..8235)
                     /locus_tag="VGY20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274229.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAVVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTIGQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8238..9944)
                     /locus_tag="VGY20_001382"
     CDS             complement(8238..9944)
                     /locus_tag="VGY20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274230.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPFLFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9937..10386)
                     /locus_tag="VGY20_001383"
     CDS             complement(9937..10386)
                     /locus_tag="VGY20_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4274231.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
BASE COUNT         3088 a         2315 c         1878 g         3146 t
ORIGIN      
        1 atatattcgc tattagccat tttttctcct ttccaacttt attattttag catattttat
       61 gacaatgatt acaatattat ttgattacaa ttgattagct aagtgaatac taaactagtc
      121 tctttcagca tataccaaaa aaatattgta cactaattga ttcataaaaa ccatttcttt
      181 aaatcacact aatcaaaacc tcaccgtttg ctgtcattat cacgtaagca tctcaccacc
      241 tctctttcaa atccaatctc caaaatagaa ataaactata ctatttttta ctttaccaag
      301 gtagcaagct cagataacga taaaaaagac tgaagaactt aatcttcaat ctttcgttgt
      361 cgtgactttt taactcctta aagcgtttaa aaataaaggt gaacgataca gccttatagc
      421 atgtttttga tgtgctcaat ggatttatca cgtcccaata aataaatcgt atttggtaat
      481 tctgggccat gcatttcacc agatactgca atacgaatag gcataaagag gtttttccct
      541 ttgattcctg tttctttttg aacagcctta atttgtggga aaatattttc tggtttgaag
      601 tcttcatctg acatagcttc caacttagcc ttgaaagctt gcaagactgt cgaaactgtt
      661 tcacctgcca tcacttcttt ttcggcttct gttaattcag ggaaatcaga aaagaaaagg
      721 tcagtcaatg gaataatctc atctgcagat tttaattgag gtttgtataa ttctactagc
      781 ttctcagctt tttctgtcaa acggcctgcc tcttcaagga aaggtttgca gagggcataa
      841 actgtttcaa aatccgcatg tttaagatac tcattgctca tccaatccat tttcttctgg
      901 tcaaaggcag ctggcgactt gctaaggcga ttttcatcaa aaagggcaat cagttgctca
      961 cgagagaaga tttcctcttc tccgccagga ttccagccaa gaagggcgat gaagttaaag
     1021 acagcttcag gcatataacc ttttttgcgg taatcttcaa tgaattggag ggtattggtg
     1081 tcgcgttttg ataatttttt acccgtttca gaattgatga taagagtcat atgaccaaac
     1141 tctggcgctt cccagccaag agcttcatag accatcaatt gttttggcgt attggcaata
     1201 tggtcatcgc cacggataac gtgagaaatt tgcatatcat gatcatcaac aacaacggca
     1261 aagttataag ttgggtagcc atctttcttt tgaataaccc agtcaccacc gatgttgcca
     1321 ccttcaaact cgatatcgcc tttaaccatg tcagtccatt tgtaaatacc ggactcatta
     1381 acagcaagac ggactgttgg aacgatacct gctgcttcac gctctgaaat gtacttagct
     1441 ttttcgtcag ctgacatacc aataaattca ttgatatagc gaggcgtttc gccagctgct
     1501 tcttggcgtt cacgctctgc tgccaattct tcctcagtca cgtaagactt ataggctttt
     1561 ccttcagcta acaatttatc aatatattgt tgataaagtg ctagacgttc agactggcgg
     1621 taattttcat gcgtttctgg gctttcatcc caatccattc ctaaccattt taggttttca
     1681 agttgagaac gttcaccatc ttcaacatga cgtttacggt cagtatcttc aatacgaatg
     1741 ataaaagtac ctccgtgacg acgggcataa aggtaattaa atagggctgt gcgggcattc
     1801 ccgatatgta agagccctgt tggacttggt gcataacgca cacgaattgg tttagacatg
     1861 cttcttctcc tgttacatat atcactcaga aacctgtggc acgaaaaaca agcaacaaat
     1921 gatgatttga gcttgccaag accttttcag acttcttcta aaatctttct tattatagca
     1981 tattttactt gcttaggcga aaaaaatcag ccctaaaaag ctgattttat gttattttga
     2041 ctaatttgaa ctagttggta gataacgttt aagagtccca gcaaattgct caaggttaag
     2101 actttggata taaatctcac catgacctct aaaggtatta acaactcctt cacctgtacc
     2161 aattgactgc ataaagccat tttctaaatg aatatcataa tcaagttctc tgctccatgc
     2221 tacaacatgg gcattatcaa tagtcattgt gccaccatca agtgtgattt tcttgataga
     2281 tccaaacgag ttagcaagta aggtacccag gccttcagtt gtcatgacaa aaagtccgcc
     2341 ttgaccacca aaaagtgctt ttccaatatt ttgacgttcc attttgtact gtgctgaacc
     2401 atctagggct agaaaggcac catcatttag acgatattgt ttttcaccta gttctaaagc
     2461 aacaatctgt cctggtgtat tgggtgctaa tgctaattta ccatctccat ttgacatagc
     2521 ttgtgtaatg aacattgatt caccagaaac catggaacgg ccgatggctc caactaattt
     2581 tcctaaacca gatcccttac cattcaattt ggtattcaag gttacattct cagtatgata
     2641 aaccatactt ccttgttgta agtaaactga tcccccatgt tccagatcaa tctcaactag
     2701 tggaaattgc atgttttggt caatcgtaaa tctcattcta ttgtcgacca tcttactctc
     2761 cttgtttgta tgaataactt gatacaatta ttataacgct tccctatttc tttgtctaat
     2821 attttcaata atttttaaaa ataaaaaaga gctgaattca gctctgcaac ttggctacta
     2881 actattccat tacttgtgtc atctttccag tatccacatc gtagacggca ccgttaataa
     2941 cgacatcatc agggataaga gaggaggctc ttagcttagc catatccttg cggacactgt
     3001 cttccacatc ttggaaggga aggaaatctt gccctgaaac atccacacct aagtgctcat
     3061 gaatatgctt ggcaaagcct tcattagtaa aagtctgcgc gccacaatct gtatggtgga
     3121 gaaccacaat ctctcgcgtt cccatttgct gctgagaaat aaccagcgac cgaatcatgt
     3181 cttcggtgac acgtccgcca gcattcctta aaatatgagc atctcctaga gccagcccca
     3241 gagcttgggc cacatgtagc cgtgaatcca tacaagtcac aatcgctact ttagtcttgg
     3301 gttttaaggg caaatgagct gtcccatgca aggccacata agcctgattg gcagccataa
     3361 aatgttcaaa ataagacatc aataaactcc ttttgagaaa cagcagagct atttttgctg
     3421 ttagtttgtc ttatcttaac atcttttatg gaaaatagct ggattaacct cttagacggt
     3481 cggtatcgtt cgaaaagtta aaaaaacaaa tgattgggat actttcttga caaatttcct
     3541 cctcatttgc tcttagtttc tgatcctcta gctaaaaact gcctttaaaa cctgaccaac
     3601 cgtagtcact ccaacaacct caataccttg agggatatca atgccttgca aggcattttt
     3661 aggagcatag accttggtaa agccaagctt tgctgcttca ttaatccgtt gctcaatacg
     3721 ggtcactcgc cgaatctcac ctgttaaccc gatctcgcct aagaaagctt cttgagggct
     3781 ggttggtttt tccttataac tggatgcgat agccacagcg actgccaaat caatggcagg
     3841 ttcgtctagt ttgacaccac cagccgactt gaggtaggca tcttgatttt gtaagagtaa
     3901 accacaacgc ttttctaaaa ctgccattat taggcttacg cgattgaaat caagtcccgt
     3961 agtggttcgt ctggcattgc caaaaacggt tggtgtcact aaagattgca cctctgctaa
     4021 gataggacga cttccttcca tcgttacaac gacagccgat cctgttgccc catctaagcg
     4081 ttcctctaaa aagacttgac taggatttaa gacctctacc aagccgccag actgcatttc
     4141 aaagatgcca atctcgttag tggagccaaa gcggtttttc acagcccgaa gaatgcgaaa
     4201 ggtgtgatgc ctttcccctt caaagtagag aacggtatcc accatgtgtt ctaacatacg
     4261 aggcccagcc aaggttcctt ccttggtgac atgacccaca ataaaggttg caatgttgtt
     4321 cgtctttgca agctgcatca attctgcagt gacttctctc acctgactga ctgatccttg
     4381 aacacctgta atatcagggc tcatgatggt ctgaatggag tcaataatta aaaaatctgg
     4441 tttaatattt tcaatttctg tgcgaatagc ttgcatattg gtttcggcat agaggtaaaa
     4501 ttcattgtca atatcaccca gacgctcact ccgcagttta atctgctctg ctgattcttc
     4561 cccagacgca tagagaaccg ttcccttatt agcgagctgc gtagatacct gcaagagtaa
     4621 ggtggatttt ccaatacctg gatcaccacc aattaaaatc aagcttccag gaaccacacc
     4681 ccctccaaga actctattaa attcagacat gtcagtctgt gttctgtgat aactaatatt
     4741 atccacatct tttagtttaa ccggtctgga cttttctcca gctagactaa cacgggcatt
     4801 tttgacttct ttgacctcta cttcttccac aaaagaagac caggccgagc aatttggaca
     4861 gcgccctaaa tactttggcg actggtagcc acactcctga cagataaagg ttgctttttt
     4921 ctttgccata gtaatctccc tcccctagac aagacaaacc cactcttaaa gaataggtct
     4981 gctgtcataa gcatttttgt taaatcgctt tactgccctg tcgaaccaaa accacctgtt
     5041 cgttcacctc tggcttggtc accatcagca attaaaaaag gcataaaaac accctgaacg
     5101 atcctctcac ccacagctaa tgtcacaggg tgatctgtaa tgttttgcat ttgtgcaaaa
     5161 atatgtcctt cattagcctc attgccataa taatctgcat caatcacacc tactgaatta
     5221 atcaggataa tccctttttt tcgtggattt gacgaacgat catagaggta aagcacctca
     5281 ccatcttgca tataggcctt aacccctgtt ggtaccaact taatctcccc tggggcaatg
     5341 gttacagcct cagcaacaga caggtcataa ccagctgcat gagtcgtctc acgttttggt
     5401 agtaaatcag gattagtaaa gcttgaaaca agctcgaaac cacgaatttt tgtcattatg
     5461 ataggtatat cctttcaagt caatcatatc ctcttattat agactattcg taaaagaata
     5521 gcaaaaaagc ggtcaacttt aaacggacca cttttaatca tgccatcaca tctcatcccc
     5581 acgataacta aagcggatgt gggtgaaatc attatccaaa tccttgtctg ccataaaagc
     5641 tttggcatct tttttcactt gaaccaagtc aataccttgc attttggcag cataaacaca
     5701 gccacaatag cattgacggt aaatatcata ctcctcacac atttcaacgg atctgcgata
     5761 accgttgttt ttcttgaaat cacttggcag gtatttggtg gtgtaaacct tttgcacatc
     5821 gatgcccaca tcattaatgg tttgcgaatt tttatgagga ctgatggtta gggcactggc
     5881 aaagtagtca aaacctagtt ccactgcttt ttgagcggtc ttgtctagtc ggtaatcaaa
     5941 acagacacgg caacggtccc caccttctgg ttcttcttct aaacctcgca cttgtcgaac
     6001 gtactcattg gggacatagt ccgcttcgag aaactgcacg gtgttgcctg ttttagcgtt
     6061 gaactcggag acaaactgtt gtgtcacata ggctctgcga tggtattcgt ccttagggtg
     6121 aatattagaa ttggcaaagt aaaccgtaat gtcagcaaac tgcgtcaggt attctagggt
     6181 gtaagtggag catggggcac agcagacatg cattaaaata ctgggacgca cggattcctt
     6241 agcccaaacc ttggccatct gttgcatgac cctgtcataa tttatctttt gattgggatt
     6301 catgttagcg agaatctctt gtaaatcaat cattgtggct actttctaat tgttttttct
     6361 cttgcttgct attataccat atcaggaaag aaagcttgac tgctttattg ttcaccaaaa
     6421 aactccgcta agacgcggag ctgataccat ctattcaaag acaaattgat tgtgatacaa
     6481 ttcggcataa aagccttttt gatgcaatag ctcatgatga tttccttgct caatgacctt
     6541 gccatctttc aacacaataa tgtgatctgc atttaaaatg gttttgaggc ggtgagcaat
     6601 gacaaagcta gttcgacctg ccacgatagc ttccatggcc cgttgaattt tactttcggt
     6661 aaccgtatca acatttgaag tggcctcatc caaaatcaac acttcagggt cagtcagtag
     6721 cgtcctagca atagaaatca actgcttttg acctgttgaa aagacattgt catcatctga
     6781 gacataggta ttgtaccctt ttggtaagga catgataaag tcatgaatat gggtcgcacg
     6841 cgcagcagtt tcaaccatgt cttgaccaat ggtctgatca ccaaaacgaa tattatccgt
     6901 aatggttcct gaaaaaagaa ctgactcttg caacacaatc cctacctttt gacgaagact
     6961 atccaaatcg tagtcacgaa tatcacggcc atcaaaggta atcgaacctg catccacatc
     7021 gtagaaacgg ttaatcaagt tcataatagt ggtctttcca gaacctgtcg gtccaaccac
     7081 ggcaatcatt ttgcccttgg gtgcaacgat tgacacatct gataaaactt tttgcccagg
     7141 aagataccca aaatcgacgt ggttaatcgc cactgcttct tttaagctgg tgaacgctgg
     7201 tgcattttgt ggacgaactt cttcggtttc atcaaacatt tcttgaatac ggtgagcacc
     7261 ggtaaaggcc agctgcaatt ctccccaact agacgcgatt tgcatcatgg gttggtaata
     7321 ttgctgggaa tattgtacaa aagtaaccac taaaccaagc gctgccgctg ctggcataga
     7381 tttgtcactg aggacaattg ttgaaccgac aaaaatcacg acagccgtgt taataaggct
     7441 cattccattc atgactggaa ataattgtcc tgagaacaga cggcgtttga aggtggcttg
     7501 tcgaaccctt tcattatgct ttaaaaaggc tgtcatcgta tcttcttgga caccttgtac
     7561 aataatagcc ttttgtcctg aaatggtttc atccataaaa gcatttaaag cactgacttc
     7621 ttgctgttgg atattggtgt attttcttgc caaacggatg ttaatcacta aaaaaatgag
     7681 agcaactggg gttgatgcga tggttaacaa agctaaacgg ctatcttgcc taaacatcat
     7741 ccagaccagg ccgatgtaaa gggcaatatt agtcaccact tgaaccaagg attggttcag
     7801 cgagttttga atattatcca aatcactcgt gaaacgagaa agaatctccc catctttatg
     7861 gcggtcaaaa aaggcgacgg ttaaacgttc taatttacca aacaagccct tgcgcatgcg
     7921 gttggtcgaa tgtgagacaa cacgtgtgag taagaaacta tatattagat tagctaaaac
     7981 agtaaagaaa taagtcatca aaagcttaaa catcacggca ttaaaagctg ataaatcagg
     8041 gctaaaatgt gtctgaccac tcatcttagc aacgtaataa gcctgaccga tttttcccaa
     8101 ctcagtcaag gactccccta agaagacagg agcttttact tgtaaataag ttgctaagat
     8161 aacggcaaca gcaatgacag taaacgagaa acgatagcgt ttaaaataaa accagaaaaa
     8221 acgtgctgtt ttcatcacta ctcctccctt cctttttgtg tctcataaat ttcacgataa
     8281 atcgcattat tagccaccaa ctctgcgtgt gttccttctc caattaactg tccttggtca
     8341 aggactaaga tcttatctgc cttaactacc gatgaaattt tttgagcaac aataactgta
     8401 gttgtacctt ctaatttatg acttaaggct tcttgaacgc gtttttcaga ttttgcatca
     8461 agagctgaag tggaatcgtc taatataagg attttgggat gattaatgac accacgtgca
     8521 atagacagac gttgcttttg tccgccagac aagttactac ctcgctcttc aacttggctc
     8581 tcataacggc tatccatgcg atcaataaat tcttttgctt gcgcgatttg agcggctttt
     8641 tgcatagcat caatatcagc ttttgctgag ccttggcgaa ggttatcagc tatcgttcct
     8701 gaaaagagaa tagccttttg caacacgatg gaaaccgatt ggcgcagggt tgtttgactt
     8761 agggttttaa tcggctttcc tcctaacaaa atttggccat cttgaggatc aaataagcga
     8821 gggatgagct gtacaagcgt tgatttccca gatcctgttg caccaacgat gccgaccatt
     8881 tgcccaggtt caatagcaaa agaaatgtgc ttcaaggttg gctcatcatc attaggatat
     8941 gtaaaactaa catcgtcaaa tacaatgctt cctgagatgg tttcttcctt ttcattttca
     9001 aatgtcatgg caggttctgt agacaatatt tgcctaattc tagccataga aacaaaagca
     9061 cgactaactt gcatccccat ggaaccaacc acaataattg aaaacataat ttgcatcata
     9121 taagtcatga aagaggcaat attcccaatc actgttggat ctgtttcgac catggtcgaa
     9181 acaacattta tagaaacata aactgccaaa taagacacca acattaaagc cggctgcatt
     9241 agtgaaaaac cataaccaat agataaattt aacgccaaaa gatcattgga cgtttcttta
     9301 aatttagtgt attgttgttg ttcttgtaca aatgatttaa ccacgcgaac gccacggaga
     9361 ttttccttag cgatgcgatt aatcttatcc attaaccgtt gaaatttacc aaaacgagga
     9421 cccatttgac gcatcaccaa acccatgatg agggcaatta agataaccat tacaacaatg
     9481 acccaccata attgagggaa tgtctgaact gccatgataa acgcgccaat aaaaaggaac
     9541 ggcaaacgaa agagaatttg aaacatcatc atgactaaac tttggatttg attgatatca
     9601 ttagttagcc taaccacaag attaccagca ttaaaagctt caatattggc atatgaaaag
     9661 tcttggattt ttcgaaaggt cttctccctc atatcagcag atacaccttg agctatttta
     9721 gcagctaaaa ccgtattaat cgctccagac attaacccta aaagggcaat aataagtaat
     9781 ggtttcccaa cctcataaat tttctcttga ttattttgaa taacagcgct tagcacagat
     9841 ttaagataat gtggttgcaa gagactactt gcaacaaccc caataaccat aaaaaatgag
     9901 cctaaagcat accatttata acgtaagatt gcgtctttaa gcatcgttgt ctcctttata
     9961 agttagcata ttttgcttga gctgttcaat tacttttttc gctgtcagca actcttcttt
    10021 tgtaatttct tttaaaaaat agtgctcgat atccttacga cactcccgca acaaaggcaa
    10081 tttatctcta ccaacctgcg ctaacactac ttgcttataa cgcttatcca cttgggaagg
    10141 catcacattg ataaacccat ttttgaccat acgtttcact aaatgactag caactgactt
    10201 tgagatacga agttgttttt caatatcttt gacaaatatt tcttgatttt ggtgtttagc
    10261 taaaaaaaca agaacataac cttgaggacc tgctagatgc tctacatcat atttttttgc
    10321 aatctcatca ctaatttgtt cgatttgatg tatcaattca cgtaaatcac ctatcacttg
    10381 tgacatctct tccctccttc ttttccttaa tagtttctat gataagt
//
LOCUS       DAUCWO010000058        10343 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUCWO010000058 DAUCWO010000000
VERSION     DAUCWO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.058"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(184..489)
                     /locus_tag="VGY20_001384"
     CDS             complement(184..489)
                     /locus_tag="VGY20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ4274232.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(501..920)
                     /gene="ruvX"
                     /locus_tag="VGY20_001385"
     CDS             complement(501..920)
                     /gene="ruvX"
                     /locus_tag="VGY20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ4274233.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(917..1186)
                     /locus_tag="VGY20_001386"
     CDS             complement(917..1186)
                     /locus_tag="VGY20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ4274234.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1300..1698)
                     /gene="spx"
                     /locus_tag="VGY20_001387"
     CDS             complement(1300..1698)
                     /gene="spx"
                     /locus_tag="VGY20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ4274235.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(1989..3125)
                     /gene="recA"
                     /locus_tag="VGY20_001388"
     CDS             complement(1989..3125)
                     /gene="recA"
                     /locus_tag="VGY20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ4274236.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPQLFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            complement(3214..4485)
                     /locus_tag="VGY20_001389"
     CDS             complement(3214..4485)
                     /locus_tag="VGY20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ4274237.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRRDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPI
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4554..5114)
                     /locus_tag="VGY20_001390"
     CDS             complement(4554..5114)
                     /locus_tag="VGY20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ4274238.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEELADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5124..5720)
                     /gene="ruvA"
                     /locus_tag="VGY20_001391"
     CDS             complement(5124..5720)
                     /gene="ruvA"
                     /locus_tag="VGY20_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ4274239.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGHTKARSNKAGNTQLDEAIEAL
                     LALGYKAKELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(5722..6942)
                     /locus_tag="VGY20_001392"
     CDS             complement(5722..6942)
                     /locus_tag="VGY20_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4274240.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLDSFRNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQLYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(6953..8935)
                     /gene="mutL"
                     /locus_tag="VGY20_001393"
     CDS             complement(6953..8935)
                     /gene="mutL"
                     /locus_tag="VGY20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ4274241.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLTLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNIFTETDHPNLDLKNRQKLSQMLTRLE
                     NEEKSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(9030..10175)
                     /locus_tag="VGY20_001394"
     CDS             complement(9030..10175)
                     /locus_tag="VGY20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ4274242.1"
                     /translation="MKIKEHKKKNGAIVYRASIYLGIDQMTGKRVKTSITGRTQTEVK
                     QKPKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRHIM
                     PTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNPARDI
                     ILPRKQPNAKKKVKFIDPSDLKSFLEHLESSQHKRYNLYFDAVLYQLLLSTGLRIGEA
                     CALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMRQR
                     QLFNEVGARVSEVVFATPTRKYFNASVRQSALATRCKEAGIERFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
BASE COUNT         3144 a         2221 c         1711 g         3267 t
ORIGIN      
        1 atttcttata tattttcaca tactgtaata attctctaaa tctatgcaaa ggtttagcct
       61 tacaattatt aataaattta tcatttaatt cttttatcat tatcacctcc tgcttattta
      121 ttcgaaaata acattaaaaa aatagccctc acggctactt tttaacatag tgtcactaac
      181 aatttagttc tcatctagaa aactattaaa tacctcttca atcatatccc attcagcatc
      241 tgaatcctca ggaatcggtt gtaaatcccc ttcagtacca tcttcatttt cagtaaatga
      301 ataggcttgg atttcaattt caccagactc atcttcttca gatcccgctg gaaccaagag
      361 aacataattt ttaccaaact cttcacgacc gtcaattgtt aataaaattt caaacaaggt
      421 ttcatttcct tgttcatcca caagtgtgat aacttcgtgt tggtggtcat tctcatgatt
      481 atgtgtcatt ttttatctcc ttaaaaattc cgatttaaat agttctgtaa aatgagttgt
      541 gcagctaatt tatcaatgac tttttttcgc ttaccacgac taatgtcagc ttgctcaata
      601 agcatacgct ctgcctcaac agtcgtcaat cgttcatctt gataatggac tggaagtcca
      661 aagaggtgct ctatgtggtt tccataagtt atacttgcat caacacgtgg accattggtg
      721 ttattcatat tttttggcaa tccaataaca aactgttcaa cttggtactg cttaacaagc
      781 tcttctaaac gtgtaaagcc aaattctgct ttttcctcat caatcttgat aatttcaagt
      841 ccttgggctg taaagccaag aggatcacta atagctacac caaccgtttt cgagccaaca
      901 tcaagtccca ttattctcat ttaacatcaa ttccatttcc ttgtaaatag tagcgaacaa
      961 gttcttctac aatttcatca cgttcatatt tacgaatctg atttcgagca tcattataac
     1021 gaggaacata agctgggtcc ccactcaaga cataaccaac aatttgatta attggattat
     1081 aacccttttc atcaagtgaa tgatagactg ctgttaatgt ttcactaatt tgtcgtttgt
     1141 cgccatcatc caacttaaag cggactgttt catctgtaaa tcccatactt acaccttctt
     1201 tctaaaatta agtgctatgc tttattatag cttatttcta ttaaataaac aagaatttca
     1261 agcgaagaag gtatttaaaa aaaagctcta caagagcttt tagagtgcag cacgtaatcg
     1321 tgcttcagta ttttcaatat tacgaataga gcgaggtaaa aaagcacgaa tatcgtcttc
     1381 tttgtagcct acttgcagac gtttatcatc aatcaaaatg ggacttttaa ggatgcgcgg
     1441 gttatcttga atcaaatcaa taacctcact aacacttaat tcttcaatat cgcaatcgag
     1501 agctttggca taacgatttt tagatgaaac aatgctctcc actccatttt cggtttttga
     1561 taaaattgct aaaatttctt ctttagttag cggttctttt cctaagttct gttctttata
     1621 agcaagctta tgggcattta gccaagtttt ggctttcttg cagctcgtac aacttgaaat
     1681 cgtgtaaatt ttaatcatgt tgctgactca ctttctattt cgtgatagct ttattgtagc
     1741 acagtttctt atctataaat ataggtcttt ggacctattt ttttaatttt aaagatatta
     1801 tcttagtaaa ataaggtttt tcacctgtat tcccttgaat tattagttat tttaatttta
     1861 tatcagttaa cctaagttct gctctctcac atctttttaa atgatggttt ctattcaccc
     1921 acacacctat gaacagtagt cctactaacc tttaaaaata cattttaaaa agccctgaag
     1981 ggctcttttt aatcttcaat ttcaatacca ttatctaaat ctaaagcaag atcatcggtt
     2041 tcttctgatg ctactgccat agcagattct tcttcgcttt cttcaagcaa accaaattta
     2101 acacgtactt tacggtcgat ttcatcaaac aattgtggat gatcggccaa ataacgctta
     2161 gcattttcag aaccttggcc aatcttctca ccattataag agaaccaagc acctgctttt
     2221 tggataatgt ccaaatcaga agcaattttc acaagctccc ctgtacgaga aataccttcc
     2281 ccatacatga tttcaacttc tgctacctta aatggcggag cgaccttgtt tttaacaacc
     2341 ttgattttgg tctccttacc aatgctgcta tctttttggt caccagttcc tttaatttga
     2401 gttgttccac gcacatccag ccgaacagaa gcatagaatt tcaaagctcg accacctggt
     2461 gttgtttcag gatttccaaa catcacacca actttttcac gcaattggtt aataaagata
     2521 gcgatagttt ttgttttatt aatagaagct gataatttac gcatggcctg actcatcata
     2581 cgtgcttgca atccgacatg gctatcgcca atatcaccat caatttcagc acgtggcact
     2641 aaagctgcta ctgaatcgac aacaaccaga tcaaccgcac cagaatcaat caatttacct
     2701 gcaatttcaa gtccttgttc tccagaatct ggttgagaca agaggagttc atcaatatta
     2761 accccaagcg cagcagcata agctggatca agcgcatgct cggcatcgat aaaggctgcg
     2821 attccacctt ctttttgagc ttgtgctaca gcatgtaaag ccacagtcgt tttaccggaa
     2881 gactctggac catagatttc gatgatacgt cctttaggat aaccaccagc tccaagcgca
     2941 atatcaagag ctagacttcc tgaactcata acctgaactt tttgctctgc acgttctcct
     3001 aatcgcatca ctgcgccctt accaaaatct ttttcaatat ttttcaaagc atcatcaagg
     3061 gctttgcgac gttcgtcgcc aaacttctta gtgatttctt cattcttttt taattttttt
     3121 gccaatactt tctcctcttt tttaacaaac catcgtcagc aggattaatc tacataattg
     3181 taaaccatat cctactatag ctactaaata acattatagc aaattctcaa gttttaataa
     3241 agttttacgg accatattaa aggcatgtaa agtggcgata tagcgcacat ccaaacgact
     3301 tcgcccgcta atcaaaacct ttattgattc taccttattt tgagtggcaa ggccaatgaa
     3361 aactgtacct gcaggctgtt cctccaacat atctggtccg gcaacacctg ttaaacttac
     3421 cccaatatca gcaccagtca ataaccttgc ttgctccgcc atcccctcgg ctgtataagc
     3481 actaacaacg ccatgcgatt tcaactcctc taaaggaagg cctagcattt tcgctttttc
     3541 ttccatgcta taagccacaa agcctccatt gaatacttga gaagctcctg gaaaactcgc
     3601 caaagttgac tgaaataatc ccgcggttag actttctgct gctgtaattg tcttatcata
     3661 ttttactaag agctcaaatg tctcacgcgc taaggaatta tcctccccat agccataaaa
     3721 gacatcagct aagggttgac cttcaagagt ttttcgggat agtagctgcg cttctagctg
     3781 acctaacttt ttatctgcca gagcttggtt ttcagttttt gttgataagc gaagagtcac
     3841 ttcgccagtc ttagcatacg gagcaatggt tggatcagtt tgattctcaa taaaatctga
     3901 caagactgtt accaactgac tttccccaat accaaaaaag cgtagtacct ttgaatacaa
     3961 tgtactgtat tgttttgaca gaagtggtac caattcttca tttactatcg gcttcaattc
     4021 acttggagga cccggtaaga caacataact aataccatcg actgtaatga acccaccaac
     4081 cgcaagacca gttttatttg gcaaagggat tgatccttca atcacttgtg cctgtcgctc
     4141 attatttggt gtccgtgatg aaggcttgcg cttagcaaaa aagtcatcta gtttctgaca
     4201 agcttgctca tcataaacca agtctctcct aaggtacttt gctaaagttt gtttggttaa
     4261 atcatctttc gttggaccaa ggccaccgca taaaatgacc aaatcactcc gctgactagc
     4321 agttgtaatc actgaaagta aacgctcctc gttgtcccca acagccgttt gaaaatagac
     4381 atcaatacct agctctgcca ttttttccga cagaaattga gcattggtat tcacaatttg
     4441 accagtcaaa atttcggtac ctactgcaat cagttcagct ttcatttccc cacctactta
     4501 tctattcgta atctgatctc attttatcaa aaaatcttcc aattcacgaa gaattagaag
     4561 attctattga aaacacaagc ctcttcatgg tcatttacca gacccgatgc ttgcataaaa
     4621 gaataaaccg ttgtaggccc taaaaattta aatcctcttt tctttaaatc cttagctaag
     4681 cgaatagata attctgtttg agcaggaaca agattctctt gattgacaag gttgttaatt
     4741 ggctttcccc caacaaaatt ccagagataa gttgaaaaac taccaaactc ttcttgaatt
     4801 ttctgtactg cgatagcatt gttgacagtt gctgctagct ttagcttgtg gcgaatgatg
     4861 gatggattct ctaaagcgtc agccagttcc tccgaggtaa aggttgcaac cgatgcaatg
     4921 tcgtaatgat gaaaaacagt tctgaaagct tggcgttttt tcaaaacagt cagccaggat
     4981 aagcctgact ggtaactttc aaggcagagt aattcaaaaa aatctctatc atcatgtagg
     5041 ggttgacccc attctaaatc gtggtaatca cagtaaagct ggttatcctt aggaacccat
     5101 gagcaacgtt tcatgtgaaa cacctaccct ttcattaaca gtttcaatgc tgatttgatg
     5161 tattgctctg ccgtctcaga ggtcccctca aagaaggcac gaattttttt cagctctttt
     5221 gctttataac cgagggcaag gagggcttca atcgcttcgt ccagttgagt attgcctgct
     5281 ttattgctgc gcgctttggt atgaccagtc tcttgtggag cctccacaaa tttgccagct
     5341 aagtcaagaa ccatttgctg cgctgttttt ttaccaattt taggaaattt cattaggtac
     5401 ttaatgtcac tgttatcaat ggcattcaca agtccctcat tatcatcaac tgccacaata
     5461 gcaagagctg ttgtcggacc aatacctgat acagaaatta atttcagaaa aacatctttt
     5521 tcatcttccg tgtgaaaacc aaacaacagg tgagcatcct cacgaatcac ttgatgcaga
     5581 taaatggtta ccaattggtt gacactatct gtaaagctat aaggattggc aacattaatc
     5641 atgtagccca atccattagc ttcaacgaca atgtattttg ccgtaatttt ggtcaattga
     5701 cctttaatat aatcgtacat attacacctt tttcattttt gcagaaacaa ccgtaaaata
     5761 aatccctagc aaagcaatga ctacaaataa gcattgcacg cctaaaggac ctgtaaaatg
     5821 gctgacagat actaggagac tagccaaaat agcacctagg ggttgtgcca ttgacacaaa
     5881 acctgtataa gatcctattt gggaatgatc catcatatca gcacgtagga cttgactagc
     5941 aggaacattt atcatttccc caaaagttaa caaaacaact gataaccaga tagcatagag
     6001 ctgcgtaaag gtaaatgcca agagcatccc tagagtaaat agtaaagaac caaccacaag
     6061 ctgaggtaat agtttccatt tttccgttaa tttatttact actgtcatga aaaggacaat
     6121 cagcaatgta ttagttaaaa ccattaatga taacatttta gaactagtta cttggaaacc
     6181 taacacagcc gttggctgaa aatacagttt caaatggact ggcacatagt tatccatctg
     6241 catccagata ctaccagaaa agatggcacc taaggtaaac aagacaaagg cacgatcatg
     6301 aaacacctga cgataatttc gaaaactatc cagcaagcct tttccatgat caaaagcatg
     6361 tgtctctggt ctagtctcat caaaatagta gtaagcaacg ccaaaacaaa gtacattgac
     6421 gagtaataaa gctactaaca aggctaaaaa atgatgatca taaaaaagcc cagatagccc
     6481 tgcaccaaac atgacggcaa tattgataaa ccaataattg atggtgtaaa caaatcgacg
     6541 attactctca tcagtcaaat caatcaacat agcttcatag gcaggaccat aaaaactaga
     6601 agcaatctct accaataaaa tacccgcaaa ggttaaccaa ggaatagccg cattaggcaa
     6661 attagctaaa atagtcaaaa accagcctag cgttgttcct actgacccaa tcataatgac
     6721 ttttttgcga ccaagagcat ctgacaaatg cccaccgtat aaagttccaa caaatcccat
     6781 caaactggta atcatcatta agaggcctgt ccaaaacgta ccaaagtaag tcgtataata
     6841 catggccata aagggaaaga tactactacc taaggtaatg gtcacaaagc gtaccagttg
     6901 cctcagctga atctgcttag gaaggtttaa aaactcttgc atctcatcct ctctaatatt
     6961 ttcctagctc tcgcaggcta gtgtgatttt cttgaattcg acggaacatt ttttccatat
     7021 ctgccttact aaaattaatc aatacgggtc taccatgagg gcagttataa ggattttgac
     7081 attgggccaa ttgcagtagc agatttcttg ctgaataatc atccaagcta tgatttgcct
     7141 tgatagatcg tttacaactc atcataatag ctaactctgc tcggtaagtt ttaatagata
     7201 cctcattggt tagaagtaac atgtcacaca tttcatagac accagatgca atctcttctt
     7261 ctttcatcca gataggatgc tccctcaaaa tgaaagtatt gtgcccataa acttctaaga
     7321 agataccaac ttcatttaag agtgccattt tctcctgtaa gttaatgaaa tccgaaccgg
     7381 aaaactcaaa caaatagggc accaacaatt gctgtaaact actgtcaacc tcacctatct
     7441 tatcacgata atattcatat ttaacccgct cctgggcagc gtgttggtca attataaata
     7501 aaccatcttt tccctgagca aagagataag ttccgtgcat ctgaccaaaa taatccaatt
     7561 caggaaatac tgatttttct tcattttcca aacgggttag catttgagat aacttttgtc
     7621 tgttttttaa gtcaagatta ggatgatcag tctcggtaaa gatattctgg ggacgacttg
     7681 cgtgtttaac cgaagaaggt cctataactt cttctgagtg gggcaacagc tctgcttttt
     7741 caacctttac actgttgtca acagcgccgg aataaaaatc agtttcaggt attttttccg
     7801 agacagccga ctctttgaca aaaaagtcat tcttttgagg atcataataa agtcccctag
     7861 actgtaaagg aagttgtgtt tgctctggtt tagagaaatg tcgtgtgctt gatttggcta
     7921 agttttccag agcatctgga atcaaatctt gttccttaag actttcagaa atcgccgtac
     7981 taattaaagc catcaactca cgctcttttg aaatacgaac ctcttgtttt gtgggatgaa
     8041 cattgacatc ggccaaatag ggatcaatct gaatatcaat cacaacaatt gggaaacgtc
     8101 caaccatgag cttagaaccg taaccatcaa gaattgctcg attaagcaag aaattcttga
     8161 tgtaacgccc attaaccaaa atcgtcatgt aattacggtt ggcgcgtgtc aactctggca
     8221 agctaacata gccggaaact tcaaaatcca aatcagcatt agagatagcc agcatttttt
     8281 tggtagtatt caaaccataa atccctgcaa ttgcttggcg taaatcgcca gttcctgatg
     8341 tctgagttaa ttggcgacca tcactaatca gtgtgaaagc aacttctgga tgtgccaaac
     8401 tcaaccggtt gaccacatct acaatgtgag ctaattctgc ctgtaaactt ttcatgtatt
     8461 tgagacgagc aggcgtgttg taaaaaaggt tttcaacctt aattttggtt ccagtaggag
     8521 ttgagatcgc ttcaagtgtc tcaatttccc caccagtagc tataagaaga gaaccgtgag
     8581 tgacttcttt tgttgctgtt tttatcgtga ttttactgat agaggcaaca gacggtaaag
     8641 cctctccacg gaagccaagt gttctaattc taaacaaatc actctgactc ttaattttac
     8701 tagtagcgtg gcgacgcaga cttaaagtta aatcttcatg agacattccc tcaccgttgt
     8761 ctgtaacctg tatcatctta aggccagact cttcaatttc aacggtaatc tggctacttt
     8821 tagcatcaat agcattctca accaattcct tgacaacact cgctggcctt tctacaactt
     8881 caccagctgc aatttggttg gcgagaactt ccggtaattc aataatgttt gtcataattt
     8941 tgtttttaac actttctaaa cgttgatata acaaggtttc attaggttta cacctctcaa
     9001 aatattaatt tgtggacttt tttgtggact tataaattat tcatagcctt ttcataatat
     9061 aaaacagctt ctttttcttt gtccttagaa agatggccat aggtatccaa agtcatgctg
     9121 atattcgcat gtcctagacg gtactgaagt tccttataac taataccagc gttcagcaat
     9181 aaactagcgt gagtgtgtct aaaagcgtga aaggtaaagc gttcaatccc tgcttcctta
     9241 cacctagtag ctaaagcgct ttgtctaacc gaagcattaa aatactttcg tgttggtgtg
     9301 gcaaacacta cctccgacac acgcgcacca acctcattaa ataattgtcg ctgtctcatt
     9361 tgatagagct ttaggcttct aagggtcttt ttatcaacac taatcactct attaccagac
     9421 tgggttttag ctgtactcaa aaacttcaaa tttttattgt aagtcttatt aatggctatt
     9481 gtaccatttt ctaggtcaat atctccccac tctaatgcac aggcttcgcc tatcctcaag
     9541 ccagtggata ataaaagttg gtagagaact gcatcaaagt aaaggttata gcgtttgtgt
     9601 tgactagatt ctaaatgttc taaaaaagac ttcaaatcag acggatcaat aaacttaact
     9661 tttttcttag cgtttggctg cttccttgga aggataatat ctcttgctgg gttgtaatct
     9721 attagcccta gcaatactcc ttgttggaga acttttcgga tgactgaacg taccgcagta
     9781 taattgacat aatatttaga taatctatta attagcattt gaatatcact agcggttatc
     9841 ttatcaacct tcatattgcc cagagttggc ataatatgtc gattaagtct agtaacagta
     9901 gcatcataag tttgaggctt tactgttaac ttataggttt caagccataa atgactaagt
     9961 tctttaaatg ttttaatcac aacttttctt tttattgtag atccattaga caggaagtca
    10021 aactgcgcgt gcttgggttt ttgcttgacc tctgtttgtg ttcttcctgt gatgcttgtt
    10081 tttactctct tacctgtcat ttggtcaatg cctagataaa tactagcacg ataaacgatt
    10141 gcaccgtttt tcttcttatg ttcttttatt ttcatgatta taaacctttc catcagcagg
    10201 caagctatta ttaaaagggt tttagattag ttcatatcat ggtataataa tggtatcggg
    10261 taaatatccg aaatattata aaagaaaatc atggtataat attaggcttt ttaagacctt
    10321 gcgagcgtgg aaaacttgcg ggg
//
LOCUS       DAUCWO010000059        10170 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUCWO010000059 DAUCWO010000000
VERSION     DAUCWO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.059"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1282
                     /gene="uvrC"
                     /locus_tag="VGY20_001395"
     CDS             <1..1282
                     /gene="uvrC"
                     /locus_tag="VGY20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ4274243.1"
                     /translation="CHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFE
                     RAAEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDV
                     NLFPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGE
                     KKQLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQ
                     GTSPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPN
                     LIIVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSE
                     EFFLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIA
                     SATSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            1467..2069
                     /locus_tag="VGY20_001396"
     CDS             1467..2069
                     /locus_tag="VGY20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ4274244.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            2194..3603
                     /gene="pepV"
                     /locus_tag="VGY20_001397"
     CDS             2194..3603
                     /gene="pepV"
                     /locus_tag="VGY20_001397"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ4274245.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFIFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(3671..5047)
                     /gene="mnmE"
                     /locus_tag="VGY20_001398"
     CDS             complement(3671..5047)
                     /gene="mnmE"
                     /locus_tag="VGY20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ4274246.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGSIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    5216..5351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            5380..5880
                     /locus_tag="VGY20_001399"
     CDS             5380..5880
                     /locus_tag="VGY20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ4274247.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            5945..6310
                     /gene="rplL"
                     /locus_tag="VGY20_001400"
     CDS             5945..6310
                     /gene="rplL"
                     /locus_tag="VGY20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ4274248.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            6568..7188
                     /locus_tag="VGY20_001401"
                     /pseudo
     CDS             6568..7188
                     /locus_tag="VGY20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            7368..7678
                     /locus_tag="VGY20_001402"
                     /pseudo
     CDS             7368..7678
                     /locus_tag="VGY20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            7676..7960
                     /locus_tag="VGY20_001403"
                     /pseudo
     CDS             7676..7960
                     /locus_tag="VGY20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8006..8218
                     /locus_tag="VGY20_001404"
     CDS             8006..8218
                     /locus_tag="VGY20_001404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274249.1"
                     /translation="MMTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLKFLF
                     NRANSLEELLLKAKEVNLTIDLKQKM"
     gene            8483..9180
                     /locus_tag="VGY20_001405"
                     /pseudo
     CDS             8483..9180
                     /locus_tag="VGY20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
     gene            9331..10017
                     /locus_tag="VGY20_001406"
     CDS             9331..10017
                     /locus_tag="VGY20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4274250.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
BASE COUNT         3284 a         1689 c         2172 g         3025 t
ORIGIN      
        1 ttgtcataca gacaaggcct attgggatcg tttaattgat gatgttaagc actttttaaa
       61 tggtaaagat gacaaaatta ttgaagatct tcgttctaag atgttagcag cttctgaaga
      121 aatggcattt gaacgtgccg ctgaatatcg agatttgatt tcaggtattg ccaccatgcg
      181 caccaagcag cgggtgatga gcaaggattt gcaagataga gatatctttg gctattatgt
      241 tgataaggga tggatgtgtg tccaagtttt ctttgtgcgt caagggaaat taattcagcg
      301 tgatgttaac ctttttccct attataacga tgcagaagaa gatttcttaa cttatatggg
      361 gcaattttac caagataaac agcatttcat ccctaaagaa gtctttattc cagaagctat
      421 tgatgaagaa ctggtagccg ctattgtgcc aaccaaaatc atcaaaccca aacgcggtga
      481 aaaaaaacaa ctggtagctt tggctactaa gaatgctcgt gttagtctgc aacaaaaatt
      541 tgatttatta gaaaaagata tcaaaaaaac gagtggtgct atcgaaaatt taggtcagtt
      601 acttaggatt gacaagcctg tgcgcattga agcctttgat aattctaaca ttcaagggac
      661 aagtccagtt gcggctatgg ttgtttttgt tgatggcaaa cctagtaaga aagattatcg
      721 taaatttaag attaaaacag tggtgggacc agatgattat gccagtatga gagaagtgct
      781 ttttcgaagg tacagtcggg tgaaaaagga gggactgcaa gctcctaact tgattattgt
      841 tgatggaggc gttggacaag tcaatgtggc caaagatgtg atagaaaaac aacttggact
      901 cactattcct gttgcgggac ttcaaaaaaa cgataagcac cagacccatg atttgctttt
      961 tggaaacccg cttgaagttg taccgttgcc gcgccgttct gaagagtttt tcttactgca
     1021 tcggattcaa gacgaagtgc atcgctttgc agttacattt catagacaag tgcgacgtaa
     1081 aaattctttt tcatcaacat tagatcatat ttcagggctt ggcccaaagc gaaaacagtt
     1141 attactaaga cactttaaaa ccataacagc gattgcatca gctacctctg aggagattca
     1201 agctttaggt atacctaaaa cagtagttga agcaatacag caacagataa cagacaataa
     1261 aaatgataga tcgtctcctt aaataagtgt tatgttttca gtactgatga ggtaaaaagg
     1321 cgctacttgt tacgcacgac atttcagttc gttattttga aactttcaga aagttgcgaa
     1381 gtgtagtgag tttatgattt taaaaactat tttatggtaa aataaaatgg tattaaaaaa
     1441 aacagattta gataggaagt tttgaaatga aatttctaga gttaaacaaa aaacgccatg
     1501 ccattaagac ctttaatgac cagccagttg actacgagga tttgcgaaca gccattgaga
     1561 ttgcaacctt agcacctagt gccaataaca ttcaaccttg gaaatttgta gtcgttcaag
     1621 agaaaaaagc tgagctagca aaagggttac ctctagcaaa taaagtgcag gttgaacagg
     1681 ctcaatatgt agtggcgcta ttttcagaca ctgatttagc tttacgttct cgtaaaattg
     1741 cccgtatcgg tgtcaaatca ttaccagacg acttaattgg ttactatatg gagactttgc
     1801 caccacgttt tgctgctttt aatgaggttc aaaccggtga gtacttagct attaatgcag
     1861 gcattgtggc tatgaacttg gtgttatcct tgacggatca aaagattgct tccaatatta
     1921 tccttggttt tgataagtca acgaccaatg agattttaga catcgatccg cgttttcgtc
     1981 cagaattatt gattactgtt ggctatagtg acgagaaacc agaaccaagt tatcgtttgc
     2041 ctgttgatga agtcatcgaa agaagataga gtcaggacaa ttagttaaat gtaatgtcca
     2101 gatgcgctac tgagcgacca ctattgttag actgatacta ttttggctta ttttaagtta
     2161 aacattatcc ctaaaacgaa taaggagagt ataatgacaa ccattgattt taaagcagaa
     2221 gttgacaaac gtaaagaagc tatgctagca gatttagttg acctattacg cattaattca
     2281 gaacgcgatg accaattagc tgatgataaa cacccatttg gcccaggtcc agttaaggct
     2341 ttggagcact tcctcgcaat ggctgagcgt gatggctaca aaacgcgtaa tattgataac
     2401 tatgcaggtg actttgaatt tggtcaaggt gatgaagtac taggaatctt tggtcacctt
     2461 gatgttgtgc cagctggtag cggttgggat acagaccctt atgagccagt gattaaagac
     2521 gatcgcatct atgcgcgtgg gtcttcggat gataagggac caaccatggc gtgctactac
     2581 gctctgaaaa tcattaaaga attgggtctt cctgtgtcta aaaaagttcg tttcattgtt
     2641 ggtaccgatg aagaatctgg ctggggggat atggattatt actttgctca taatggcttg
     2701 aaaaatccag attttggttt ctcaccagat gctgaatttc caattatcaa tggtgagaaa
     2761 ggaaatatca ccgaatacct ccatttcgct ggtgacaaca agggcgcttt tgtgcttcat
     2821 cgcttccaag gtgggttgcg tgaaaacatg gtaccagaat ctgcaacagc tgtcattacg
     2881 gcaccgcatg accttgatgt gctagaagca gctttggagc aatttttatc agaacatggt
     2941 gttaaaggtt ctatgaaggc tactgatggt aaaattgaag taactattat tggtaagtct
     3001 gctcacgggt cgactccaga agcgggtgtt aatggtgcta ccttgttggc taaattcctg
     3061 aatcaattta tctttgaagg ggctgctaag gattatttac atgtggcagg tgaggtgctt
     3121 catgaagact ttgcggcaga aaaactaggc cttgcttata ctgatgaccg tatgggagcc
     3181 ctgagcatga acgcaggtgt cttcaccttt gatagtcaat cagctgacaa taccatcgcc
     3241 ttgaatttcc gctatcccaa aggaactgat gcggcaacct taaaagcagg attggaaaaa
     3301 ttaccaggat taactaaggt aagcctttct gagcatgaac atacacctca ttatgtgcca
     3361 atggatgatg aattagtagc taccttactt gctgtttacg aaaaacaaac aggtcttaaa
     3421 ggctacgaac aagttatcgg cggaggaacc tttggtcgtt tgctagaacg tggtgtggcc
     3481 tttggagcga tgttcccagg cgatgaaaac actatgcacc aagctaatga atatatgcct
     3541 ttagaaaata tttatcgctc agcagctatt tatgctgaag caatctacga attaattaaa
     3601 taagatgctt atgacagcaa aaagagctgg aacactgttc cagctctttt gtgatgatta
     3661 gagactgttc ttatttacca agacaaaatt ggctaaagag ttgggtaatg agttcatcag
     3721 gagcagcatc tcctgtgatt tctccgagaa tttcccaagt tctggttaaa tcaacctgta
     3781 gtaaatcaac tggcatgcca agcgccaagc cgtcattgac agcttctaaa ctctggactg
     3841 ccttttcaat caaagaaatg tggcgggcat ttgacaggta agtggcatct tgctctacta
     3901 gtccagcatt atcaaagaag agctggttga tacgatcttc gatcagatta atgttttggt
     3961 tggttaaaac ggaaatggga atatagtcat ctggtaattg ctctaattca atcttttgtt
     4021 ccaaatccgt tttattcaac aatataatac ggttactgtc ctgactgaga tttaacaaag
     4081 cccggtcttg gtcggtcagt ttttcagaag cattcagcac aagaagaaca aggtcagctt
     4141 cttgaagagc ttttttagac cgttcaactc cgatttgttc aaccagatca tctgtctcac
     4201 gaataccggc cgtatcgaca agttttagag gaactccctt gatattaacg tattcttcaa
     4261 tgacatctcg ggtggttcct gcaatatcag tcacaatagc cttgtcttct cgcaacaaat
     4321 tattaagtaa gcttgactta ccgacgtttg gacgaccaat aatagctgtt gataagccct
     4381 cacgcagaat tttaccgcgt ttagctgttc gaaggagatt ttccaaaagg ctttggaatt
     4441 cttgtgtttt ctcacgtagc agagcagtgg tcatttcttc cacatcgtcg tattcagggt
     4501 agtcaatatt aacttcgact tgagctaagg tgttgagaat ttcttgtcgg gtgtcgttga
     4561 taagctggga aagggagcca tccagttgct tgactgcaat cgtcatagcc ttgtctgttt
     4621 tggcacgaat aatgtccatg acagcctcag cttgagtcaa atcaactcgc ccatttaaga
     4681 aggcacgttt ggtgaattcg ccaggctctg ccattctagc gccttggcga atcaatagtt
     4741 gcaagatttc atttgtcaca gcaatactac cgtgcgtatt gatttcaacc acattttcac
     4801 gggtaaaggt tttgggggct agcatgactg acaccataac ttcatcgata atagttcctg
     4861 tcttaggatc aatgatatgg ccatagttaa tggtgtgtga ggcgacttgc tctaagttct
     4921 ttcccttaaa aacagattgt gcaatggcaa gcgcatctgt ccctgataaa cgaacaatgc
     4981 caatagctcc ttcaccaaga ggggttgaaa tggcggtaat tgtatcaaat tctttagtta
     5041 tactcatgcc ctctatttta aatcaagtcc tagtagaaag caaggtatta ttttgtatag
     5101 aaaataggac tagcaccatt ttgagtttag ctaatactaa aaaaataaat gtaaaaaaat
     5161 cattttttaa taatttttat tgacaatagt attataaaag aatataatga tataggtttt
     5221 gataaaaaac tgacctaaga cagcagggga gcatgctcat aatattcctg ccgaggcaca
     5281 aaacgtgatt aaagaaaata acgtatttgt actttcgtgt ctaggtttag gtgcgatttt
     5341 tttgtaccta gcccaaaaat aaaaacggag gtaaaactaa tgagtgaagc aattattgct
     5401 aaaaaagctg aacaagtcga acttattgct gagaaaatga aagcagcagc aagtatcgtt
     5461 gttgttgatt cacgtggtct tacagttgat caagatactg ttcttcgtcg ttcacttcgt
     5521 gaaagtggcg ttgagttcaa agttatcaaa aactcaattt tgactcgtgc agctgaaaaa
     5581 gcaggtcttg atgagttgaa agatgtattc gtaggaccat ctgcagtagc attttctaac
     5641 gaagatgtta tcgcaccagc taaagttatc aacgacttta ctaaaactgc tgacgcactt
     5701 gaaatcaaag gtggtgcaat cgaaggcgct gtttcttcaa aagaagaaat ccaagcactt
     5761 gcaacattgc caaaccgcga aggaatgctt tctatgcttc tttctgtact tcaagctcca
     5821 gtccgcaacg ttgcatacgc tgtcaaagct gttgcagaaa acaaagaagg cgctgcttaa
     5881 gacgtctcag agtagcttaa ggctacgctt aacattttaa attaattatt tggaggaaat
     5941 cacaatggca ttgaacattg aaaacattat tgctgaaatt aaagaagctt caatccttga
     6001 gcttaacgat cttgtaaaag ctatcgaaga agaatttggt gtaactgcag ctgctcctgt
     6061 agctgctgca gctgctggtg gtgctgaaga agctgctaaa gattcattcg acgttgaatt
     6121 gacatctgct ggcgacaaaa aagttggcgt tatcaaagct gttcgtgaaa tcacaggtct
     6181 tggtcttaaa gaagctaaag gtcttgttga tggagcacct gctaacgtta aagaaggcgt
     6241 tgctgctgca gaagctgaag aaatcaaagc taaacttgaa gaagctggag caacaatcac
     6301 tcttaaataa gaagcagata gcaatttgag agcgaaatcc cgatttatca gttttttaga
     6361 gcttatagcg atttgaagaa actgataaaa tgatttggtt tcttgccaaa aatcctgcca
     6421 aaaatttttt ggcaggattt ttattttcta taaaaattaa gctgagtaga gtggctattc
     6481 tatttatatg tgtctagtaa taattttttt atgttcagga ggaaaatcat gcaaatgaat
     6541 gaacagtcat cacgacaaat ggtgattgcc atctatctca tttattccaa ctcaaaatga
     6601 tagtactcct tggctgttcc aagtcgaaat tgttagtgat taagaaaacc tacgagacta
     6661 tggtttgatc gatgaaggcc agcagtctcc cagtgaaaaa gggcgtttgg ctaataagat
     6721 ttatttagga aagttggaac atgattccac cccagtctta agttcagacg ggggtagtct
     6781 aaaaaaacac gggaccagtc tcaaaaacag acaagccccg tgttaaattc agcccctagt
     6841 gagactgaaa agagtgaaac tgaaggtagt gaaactaaag agagtaattt agtcattgag
     6901 gacgaggagg aaagaaagga gtgcactagt gtgaaaaaga ctgagggtca ctttacccgt
     6961 caagttgatc aggtcaccaa atatgacaaa gattatattt ggagtttggt tcattcccaa
     7021 ttacgtgaag gtggtttatc acaagctgct agtgacttag tcatgagcta ttttgaagaa
     7081 cgctatgctt atgcgctgga acacatacgg ttcgcaagaa cagctgaagc tattgcggaa
     7141 tatgtcttta atggtgtctt atccgaatgg accaagcaac taaggtgaca agaagcgaag
     7201 tgaggtgagt aagatgtttt ggtggctatt attaggtgtt tggggtctaa ggacaatttg
     7261 gttgattatt gaagttatca ctgcaccaga aatggaagat tatagttgag taaggacgtt
     7321 acataagcag taaggtcctt ttattttgtc agaaaggaat gagagatatg aagtttttag
     7381 atttgtttgc aggaattgga ggctttcgat tgggtttaat taatcagtgc catgagtgta
     7441 ttggcttttg tgagattgat aagtttgcga ggcaatctta caaagccatc tatgagaccg
     7501 aaggagaaat agaatttcat gacattagac aagtcacaga ccaagacttt agacaactta
     7561 gagggcaagt ggatatcatc tgcggtggtt tttcccttgt caagcatttt cactcgcagg
     7621 cagacgactg ggatttgagg gcactaggag aactctcttc tttgagatta ctcgagcgac
     7681 tcatacagac aaagatcata ttcataatca catcttaatc aacgccattg actgtaattc
     7741 agataaaaag ttgatatgga attatgcctt ggaacgaaac cttcgtatga tttcagaccg
     7801 catttctaaa atggctgggg cgaaaattat tgaaaagcat ttctcttacc gtgactatca
     7861 aaaatataga gcgactagcc ataagtttga actaaagcaa cgtctttatt ttttgatgca
     7921 gcagtcaaag ttctttgatg attttttaga aaaagcagag taattacatg ttcatattga
     7981 ttttagtcag aagcatagca gattcatgat gacggatcga gtaatgaaaa aaccaattcg
     8041 aggtcgtcaa ctcagtaaac gagatttata tgatgaagaa ttttttcgta cacattttgc
     8101 caagatagaa attgaaagtc gattaaaatt tttgtttaac cgtgccaatt cattagagga
     8161 gctactatta aaagcaaaag aagtgaatct aaccattgac ttaaaacaaa aaatgtaact
     8221 tttatcctag aagaagatac tcaaaagata agtttaggtc ataaaaaaat aagcgataag
     8281 aaattatatg atgtcaattt tttcaagatt actttaaaaa taaggaagtc ggtgtttcag
     8341 atcgattaga aagtctacag aaacagtaca tgcttttcaa gaagaatgag ataaagagga
     8401 ggtatccact gaagagagta aggaagtctt caagaaatac aaggggcaac gagatgccat
     8461 ccatgagttt gaagttgaac ttgtggaaca tcaaattgag aggttaattg ctgaggacat
     8521 ttatatcaag gtgtcttttg gtgtaaagca gagtggtctt attttcattc ctaactatca
     8581 attggatatt aggaaagaag aaaatcataa aaaatataaa gtctatattc gtgagaccgc
     8641 acaattcttc atttacaaca aggaggcgtc taaacttaat cgctatgtgc gagggcgtga
     8701 gctcatttac cagttaacca atgatagcaa gagtattcca aaaagaagac gaccaaaacc
     8761 attgatacac ttaagaaaaa gattgaagaa ataagtctcc tcattgagtt agatacggag
     8821 aataagcctt atcaagatat taaagatgac attgttaaag atatggctca attagatttg
     8881 accattacag agttgcagga ccatattgct catctcaata aggttgcgga ggtcttgctt
     8941 aatctgaaca acaacgatat agagaaccgc cgtttggcca gatatgacta tgccaagatg
     9001 aacttgactg cagctataaa aatagaagaa gttgagaaag agattgaaac ttctcaaaat
     9061 gaacttaata tatccataga tgagtatgaa tatctagtaa gaaggttgga aaagtttgga
     9121 gagatcttga gtgatagcaa aattatcgat acttctcgaa atgaaataca atgggagtaa
     9181 ttatgatata atttgggtgg tagcaatttt tatatcagat attaaaatct agttgatgtt
     9241 tcgtaaataa ctatattaaa gaaatgattc ctgccgctta cattaagaat agtttatttg
     9301 taagtatttc attatttaaa aggggtaata atgtataaga ttttagccat agatgatgat
     9361 aaagaaattc tgaagcttat gaagactgct ttggagattg aaaattatca tgttataacc
     9421 tgtcaagaga tagaattacc aatagttttt gatgatttta aaggatatga tttgatctta
     9481 ttggatatca tgatgcctaa tataagtgga actgagtttt gttataaaat tcgggaagaa
     9541 gttcattctc caattatttt tgttagcgct ttagatggcg ataatgaaat tgtccaagct
     9601 ttaaatatag ggggagacga ttttattgtg aaaccattta gcttaaaaca attcgtagct
     9661 aaagttaact ctcatttgaa gagagaagag agagcaaaga taaagaatga ggctgaggag
     9721 agagtgaagc gcagttttcc acctatagaa atctatctag aggaacgtat gttatatatt
     9781 gataaacagc cgttattcct gacttataga gagtacgaaa ttttagaatt actgtcacgt
     9841 catccgtata aagttttcac aaaagaagag atatatgaac aagtatatag cgatgaagct
     9901 tcagcattgt ttcattctat ttcagaatat atctatcaga ttagaatgaa attttcaagt
     9961 tttggaatta atccaataaa aactattcgc gggattggtt ataaatggga tgtttaaaaa
    10021 atattcaata aaaaatcgtg tttggcgcgc cgtcgttgaa attgtctttg gagtttgtat
    10081 cactgtttta atgattgcga taatatcgct ttctttttca aagttaaata tagtgacatc
    10141 tcataatgtg ggagaagaat tttatattaa
//
LOCUS       DAUCWO010000060         9429 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUCWO010000060 DAUCWO010000000
VERSION     DAUCWO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.060"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(238..585)
                     /gene="ssrA"
                     /locus_tag="VGY20_001407"
     tmRNA           complement(238..585)
                     /gene="ssrA"
                     /locus_tag="VGY20_001407"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            677..1291
                     /locus_tag="VGY20_001408"
     CDS             677..1291
                     /locus_tag="VGY20_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ4274251.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(1418..2203)
                     /locus_tag="VGY20_001409"
     CDS             complement(1418..2203)
                     /locus_tag="VGY20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274252.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNVNELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHNHIFNIN
                     TSADSFPFTEQKTTYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2213..2911)
                     /locus_tag="VGY20_001410"
     CDS             complement(2213..2911)
                     /locus_tag="VGY20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274253.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(2911..3282)
                     /locus_tag="VGY20_001411"
     CDS             complement(2911..3282)
                     /locus_tag="VGY20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4274254.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            3467..6577
                     /locus_tag="VGY20_001412"
     CDS             3467..6577
                     /locus_tag="VGY20_001412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ4274255.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELTDCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQELTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHLPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDAKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            6657..7670
                     /gene="pfkA"
                     /locus_tag="VGY20_001413"
     CDS             6657..7670
                     /gene="pfkA"
                     /locus_tag="VGY20_001413"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ4274256.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            7733..9235
                     /gene="pyk"
                     /locus_tag="VGY20_001414"
     CDS             7733..9235
                     /gene="pyk"
                     /locus_tag="VGY20_001414"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ4274257.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         2972 a         1568 c         1915 g         2974 t
ORIGIN      
        1 ttccccataa cgcatgcgct atggggaatt tagtactatt ttgattcttt tttttagata
       61 ttcatgaaaa aaatggtcaa aaagctctga tatgacaagg ttttgagtgg tattgatgga
      121 gtatcggtaa gcgtgatggt atggctatag ggataacgga aactcatgaa caagacaaaa
      181 agaaaaaacc ttgatgtaac aaggttttag taagttatga ttacttacgg taagcattga
      241 tggagctggt gggagtcgaa cccacgtcca aacacctgcc aacatatttg tctacgtcca
      301 taggttatgt cttcatttaa caaactaaag acacataact caagccatta gtttgcgagt
      361 ctatcaatct cttgtcacga aactagacaa tttcgtaacc gtagcttgct attttgagac
      421 ttctaacagg acacaagcaa tcttgtagaa gtcacgctag ctggttttta ggcagctaaa
      481 gcgtaagagt ttgtattttt tgcagttata tttaactggc aatttacatc tgccgatggg
      541 acgcaaaaca tgcctcataa tgcctgtcga atccgtaaca accccaaata gataattaac
      601 caatttctta cactaactat tctatcaaaa ctttttttaa atagcaaaat tttacagctt
      661 atggtatgat tagtctatga caaaacgtaa ttcaaaagcc tatatattat ggcaaaaaat
      721 tatcaaaatt ttaggaatta ttgcattaat tggtactttt ttcctcgctt tttggctgta
      781 tagactaggt atattaaacg atagtaatgc tcttaaagac ttagtgcagc gctatagatt
      841 atgggggccg tttgttttta ttgtcgtgca gatcatacag attgtttttc ctgtgattcc
      901 tggaggcctt acgacagttg caggtttttt aatctttggc cctgtaacag gttttattta
      961 taattatgta ggcatcatta ttggcagtat agtcctcttc ttgctcgtta agacttatgg
     1021 tcgtaaattt atcttactct ttgtaaatga caaaacattt tacaagtatg aaaggcgttt
     1081 ggagacacca ggatatgaaa aattatttat tttttgtatg gcatcgcctg tttctccagc
     1141 tgatattatg gttatgatta cgggattgac ggacatgtct ttaaagcgct ttgtaactat
     1201 tcttttaata actaaaccaa tttctattat cggctacagt tatttattta tatttggaaa
     1261 agatgttata tcttggttct tacagcatta aggtttggtg ttaatttgat aaaccgcttc
     1321 tatttgatgg aagacggttt tttaatttac tatgaacact gaaaaaaact ttcttttaaa
     1381 ctatagtgtg tcatagttta aaagaaagtc ttaacgctta ctgtaggttg agtttatttt
     1441 tgataataaa attagtggcg aggtaaaaca ttatcatttc aataaattgt tccaaaataa
     1501 gaagataagt tgttttttgc tcagtaaatg ggaaactatc ggcacttgta ttaatattaa
     1561 agatgtgatt gtgaacatat gttgcagcaa cgctaatcag aataactaat ataaaatatg
     1621 caataaaggc catcaagcct cgccgattag aaaaaagttg acctatagaa atggaaagat
     1681 aaattaataa gatactcgta aatgttgata ataggacata ggctagtaca gttagcatat
     1741 tgatgaaata atccatctta aaactattaa agaatccttc taatagttcg ttgacgttaa
     1801 acattggtac aattacaata gcgatagcaa atgcaaggat gatagtattg aaaacactgc
     1861 aaataaaaga agctagtagt tttgataaaa taatttgatg cgaattgacg ggtaaagtca
     1921 acgttaagta tccttcccgt ccaaaaacac ttttgctaaa acgtttgata atgataagta
     1981 atgttgataa gagggagcca gcgatcaggg agccaaaagt taaacctaat gtcagaggca
     2041 acatcttatt ggttagtact ccaaatagtc catcactatt gctttgagca aacagtttta
     2101 tcgtaaatga caaaatggca gcgatggcaa taacgaaagc attaagcgca aagtaccatt
     2161 ttccaataga tctaaattcg tattttaata attttccaaa catgatatcc tcctaaacct
     2221 tataagtttc cctaaagagg gaatcaatgg attgctggta ttcttgtctt agatcatcag
     2281 cattgccaga taagaaaagt cttccttgtt tcaagaaaat gacttcgtca aggataggtt
     2341 caatgtccga aatcaagtgg gttgagataa taacagaagc attttcacaa taactattaa
     2401 tgatggtttt taggatgtag tctcttgcgg cagggtcaac tccaccaata ggttcatcaa
     2461 gtacatagag tctggcttta cgactcatca caagaattaa ttgtactttt tctttgtttc
     2521 ctttggatag agttttgaag cggtcttcag ggtctagttc taaatctctt agaaggcttg
     2581 ttgctttgga tttgtcaaag tcgctgtaaa aatcactaaa gaactccaac atgtccttaa
     2641 ttctcatatt ttcattgaga taagtagtat ctggtaaata agaaataatt ttttttgtct
     2701 caacacaggg acggtagcca tcaatgacaa tttctccttt atttggttgt aaaaggccat
     2761 taatgagttt aattaaagtt gttttaccac ttccatttgg acctaataat ccgataattt
     2821 taccgttcgg gatagtaatg gttaagtcat caatagcttt tttttcacgg taagattttg
     2881 aaacatgatg tagttgcaat aggtgtgcca ttattgtgaa tcctccttta aaaagctcgt
     2941 caaaacaggg ataatctcag tgtgactaaa tcccattttt gtcatattgg tgataaatga
     3001 ttctaattca ctaattgcta actctcgtct tttacgagca attaattttt gatcgtcagt
     3061 cacaaaacga ccagcagtac gttgcgaata aaccattcct tcccgttcaa gttctgtaaa
     3121 cgctcgttgc atggtgttag gattgacacc tgcgatttct gcatattcac gaacagttgg
     3181 aagttgatca ccactcttga tttcttggct aataatttgc atcatgacat gttgtgctat
     3241 ctgcgcataa ataggagatt tttcatcaaa tttccaagac atgtggtctc ctttctgagt
     3301 aatatagata tttgtactaa ttaaatggta cagaaaaaaa aagggaatgt caagttttat
     3361 gccatttttc actctttgag aagtagacgt agattagaga gaaaattgaa gcaagaaaac
     3421 ctttttcata gtataatagg gaacaagata accaaaggag gaacttatgt ttgctcaact
     3481 tgatactaaa actgtatact catttatgga tagtttaatt gacttaaatc attattttga
     3541 acgagcaaag caatttggtt accacaccat aggaatcatg gataaggata atctttatgg
     3601 tgcttaccat tttattaaag gttgtcaaaa aaatggactg cagccagttt taggtttgga
     3661 agtagagatt ctctatcaag agcggcaggt gctccttaac ttaatcgccc agaatacaca
     3721 aggctatcat cagcttttaa aaatttccac ggcaaaaatg tctggcaagc ttcatatgga
     3781 ttacctctgc caacatttgg aagggatagc ggttattatt cctagtaagg gttggagcga
     3841 tacattagtg gtcccttttg actactatat cggtgttgat cagtatactg atttatctca
     3901 tatggattct aagaggcagc ttatacccct aaggacagtt cgttattttg cgcaagatga
     3961 tatggaaacc ctgcacatgt tgcatgccat tcgagataac ctcagtctgg cagagacccc
     4021 tgtggtagaa agtgatcaag agttaacaga ttgtcaacaa ctaaccacct tctatcaaac
     4081 acactgccct caagctctac agaatttaga agacttagtg tcaggaatct attatgattt
     4141 cgatacaaat ttaaaattgc ctcattttaa tagagataag tctgccaagc aagaattgca
     4201 agagttgact gaggctggtt tgaaggaaaa aggattgtgg aaagagcctt atcaatcgcg
     4261 cttactgcat gaattggtca ttatttctga catgggcttt gatgattatt ttttgattgt
     4321 gtgggattta cttcgctttg gacgcagtaa aggctattat atgggtatgg gacgtggctc
     4381 ggcggcaggt agtctagtgg cttatgctct gaacattaca gggattgatc cagttcaaca
     4441 tgatttgcta tttgagcgct ttttaaacaa agaacgttat agcatgcctg atattgatat
     4501 cgatcttcca gatatttacc gttcagaatt tctacggtat gtccgaaatc gttatggtag
     4561 cgaccattcg gcgcaaattg tgaccttttc aacctttgga cctaaacagg ctattcgtga
     4621 tgttttcaaa cgattcgggg ttccagaata cgaactgacc aatctcacta aaaaaattgg
     4681 ttttaaagat agcttggcta ctgtctatga aaagtcaatc tcttttaggc aggttattaa
     4741 tagtagaact gaatttcaaa aggcttttgc cattgccaag cgtatcgaag gaaatccaag
     4801 acaaacgtcc attcatgcag ctggtattgt gatgagtgat gataccttga ccaatcatct
     4861 tcctctaaaa tcgggcgatg acatgatgat cacccagtat gatgctcatg cggtcgaagc
     4921 taatggcctg ttaaaaatgg attttttggg gttaagaaat ttgacctttg ttcaaaaaat
     4981 gcaagagaag gttgctaaag actacgggtg tcagattgat attgcagcca ttgatttaga
     5041 agacccgcaa acgttggcac tttttgctaa aggggatacc aagggaattt tccaatttga
     5101 acaaaatggt gctattaatc ttttaaaacg gattaagcca caacgttttg aagaaattgt
     5161 tgccactacc agtctaaata gaccaggggc aagtgactat accactaatt tcattaaacg
     5221 aagagaagga caagaaaaaa ttgatttgat tgatcctgtg attgctccca ttttagagcc
     5281 aacttacggt attatgcttt atcaagaaca agttatgcag attgcacaga tttatgctgg
     5341 ttttacgtta ggcaaggccg acttgttaag gcgtgccatg tctaaaaaaa atctacaaga
     5401 aatgcaaaaa atggaagaag actttattgc ttctgctaag cacctaggga gagctgaaga
     5461 aacagctaga ggacttttta aacggatgga aaaatttgca ggttatggtt ttaaccgcag
     5521 ccatgccttt gcctattcag ctttagcttt tcaattggct tatttcaaag cccattatcc
     5581 ggctgttttt tacgatatca tgatgaatta ttctagcagt gactatatca cagatgctct
     5641 agaatcagat tttcaagtag cgcaagttac cattaatagt attccttaca ctgataaaat
     5701 tgaagctagc aagatttaca tggggctgaa aaatattaag gggttgccaa gggattttgc
     5761 ttattggatt atcgagcaaa gaccatttaa tagcgtagag gattttctca ctagaactcc
     5821 agaaaaatat caaaaaaagg ttttccttga gcctctgata aaaataggtc tgtttgattg
     5881 ctttgagcct aaccgtaaaa aaattctgga caatttggat ggtttactgg tatttgttaa
     5941 tgagcttggt tctctttttt cagattcttc ctttagttgg gtagatgcga aagattactc
     6001 agcaactgaa aaatattctt tggaacagga gatcgttggg gttggcatga gcaagcatcc
     6061 tttaattgat attgctgaga aaagtaccca aacttttact cccatttcac agttagtcaa
     6121 agaaagcgaa gcagtcgtac tgattcaaat agatagcatt aggatcatta gaaccaaaac
     6181 aagtgggcag caaatggctt ttttaagtgt gaatgacact aagaaaaagc tcgatgtcac
     6241 actttttcca caagagtatg ctatttataa agaccaatta aaagaaggag aattctatta
     6301 cttaaaaggt agaataaaag aaagagacca tcgactgcag atggtgtgtc agcaagtgca
     6361 aatggctatt agtcaaaaat attggttatt agttgaaaac catcagtttg attcccaaat
     6421 ttctgagatt ttaggtgcct ttccaggaac gacaccagtt gttattcact atcaaaaaaa
     6481 taaggaaaca attgcattaa ctaagattca ggttcatgta acagagaatt taaaggaaaa
     6541 acttcgtcct tttgttctga aaacggtttt tcgataaaaa tggacaatta gtgaagtatt
     6601 ttgcaaatga atgtgctata ataagaccgt taaattaatt aaaggagtac cagaaaatga
     6661 aacgtattgc tgttttaacc agtggcggcg acgctcctgg tatgaatgct gctattcgtg
     6721 cagtcgttcg taaagcaatt tcagaaggaa tggaagttta tggtatcaac cgtggctatg
     6781 caggtatggt tgatggcgac attttcccac tgggttcaaa agaagttggt gataaaatct
     6841 cacgtggtgg aactttctta tactcagctc gttatccaga atttgctcaa cttgaaggtc
     6901 aattggctgg tattgagcaa cttaaaaaac atggtattga gggtgttgtt gttattggtg
     6961 gggatggttc ttatcatggc gctatgcgct tgacagagca cggcttccca gcggtaggta
     7021 tcccaggaac aatcgataac gatattgcgg gaactgacta cactatcgga tttgatacag
     7081 cagttaacac agcagttgaa gctattgata aacttcgtga cacctcatca agtcacggac
     7141 gtacttttgt ggttgaagtg atgggtcgca atgcgggtga tattgctctt tgggcaggta
     7201 ttgctagtgg tgctgatcaa atcattgttc cagaagaaga gtttgatatt gaaaaagtgg
     7261 catcaacaat ccaatatgat tttgaacaca aaggtaaaaa tcaccacatt atcgtcttag
     7321 ctgaaggtgt tatgagcggt gaagcttttg ctcaaaaact gaaagaagct ggcgataaga
     7381 gtgaccttcg tgtgacaaat cttggtcaca ttcttcgtgg gggttcacca actgctcgtg
     7441 accgtgtcat tgcttcttgg atgggttcac atgctgttga gctacttaaa gaaggcaaag
     7501 gtggtttggc tgttggtatt cacaatgaag aacttgttga aagcccaatc cttggtactg
     7561 cagaagaagg tgctttgttc agcttgactg aagaaggcaa gattattgtt aataatccac
     7621 acaaagctcg tttagacttt gcagctttga accgttcatt gtcacaataa gtttagttaa
     7681 aattgttaaa tcgtcaatta ttgacaaaaa aataagggag tttcagagta aaatgaataa
     7741 acgcgtaaaa atcgttgcaa cacttggtcc tgcggttgaa atccgtggtg gtaaaaaata
     7801 cggagaagat ggctactggg ctggtcaact tgatgttgaa gaatcagcaa aaaaaatcgc
     7861 tgaattaatt gaagctggtg cgaatgtttt ccgtttcaat ttctcacatg gtgaccacaa
     7921 agaacaaggt gaccgtatgg caactgttcg ccttgcagaa gaaattgctc gtcaaaaagt
     7981 tggtttcctt ttggatacta aaggtcctga aatgcgtacg gaattgtttg cagatgatgc
     8041 taaagaattc agctatgtca ctggagaaaa aatccgtgta gcgactacac aaggtattca
     8101 atcaactcgc gatgttattg ctttgaacgt tgctggtagc cttgacatct acgatgaagt
     8161 agaagtaggt catactattc ttatcgatga tggtaaactc ggtttgaaag tgatcgacaa
     8221 agatatcgca acacgtcaat ttatcgttga agtagaaaac gatggtatta ttgctaaaca
     8281 aaaaggtgtt aatatcccta acactaaaat tcctttccca gcacttgcgg aacgcgacaa
     8341 tgctgatatc cgttttggtc ttgaacaagg acttaacttt attgcaatct catttgttcg
     8401 tacagccaaa gacgttgagg aagttcgtga aatctgtcgc gaaactggta acgatcatgt
     8461 gcaattattt gctaagattg aaaaccaaca aggtatcgat aatcttgatg aaatcatcga
     8521 agctgctgat ggtatcatga ttgcacgtgg cgacatgggt atcgaagtac catttgaaat
     8581 ggttccagtt ttccaaaaaa tgattatcac taaagtgaat gcagctggta aagcagttat
     8641 cacagcaaca aatatgcttg aaacaatgac tgaaaaacca cgtgcgacac gttctgaagt
     8701 atctgacgtc ttcaatgctg ttatcgatgg tactgatgca acaatgcttt caggagagtc
     8761 agctaacggt aaatacccgg ttgaatctgt tcgtacaatg gcgactattg atcgtaatgc
     8821 acaaactctt cttaacgaat atggtcgttt agattcatct gccttcccac gtaccaataa
     8881 aacagatgtt attgcttcag ctgttaaaga tgccactcat tcaatggaca tcaaacttgt
     8941 tgtgactatt actgagacag gtaatacagc tcgtgcgatt tctaaattcc gtccagatgc
     9001 ggatatctta gctgtaacat ttgatgaaaa agtacaacgt gctttgatga ttaactgggg
     9061 tgttattcct gttcttgctg aaaaacctgc atctactgat gacatgtttg aagtagcaga
     9121 gcgtgttgct gtggaggcag gcttagttca atctggggat aatattgtta ttgttgctgg
     9181 tgttccagtt ggtactggtg gaacgaatac tatgcgtgtt cgtacagtaa aataaatgtg
     9241 ttataactat ttacctaaag gttagaaggc aagcttctaa ccttttttga tatcaaaaat
     9301 atcatgttaa ttcactagtg ttaatttact ttaatgagtc taagtttgta ttttatacag
     9361 aatgagtaac gtttattggt attgatcaaa aattgtttac tttttttcta gcttagaaca
     9421 tggtataat
//
LOCUS       DAUCWO010000061         9388 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUCWO010000061 DAUCWO010000000
VERSION     DAUCWO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.061"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            90..1010
                     /gene="lmb"
                     /locus_tag="VGY20_001415"
     CDS             90..1010
                     /gene="lmb"
                     /locus_tag="VGY20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ4274258.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1023..3494
                     /locus_tag="VGY20_001416"
     CDS             1023..3494
                     /locus_tag="VGY20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ4274259.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARSSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPFDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            3663..3863
                     /locus_tag="VGY20_001417"
     CDS             3663..3863
                     /locus_tag="VGY20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ4274260.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3943..4569)
                     /locus_tag="VGY20_001418"
     CDS             complement(3943..4569)
                     /locus_tag="VGY20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274261.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHDFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4553..5356)
                     /locus_tag="VGY20_001419"
     CDS             complement(4553..5356)
                     /locus_tag="VGY20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274262.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIRQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSRGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5368..6189)
                     /locus_tag="VGY20_001420"
     CDS             complement(5368..6189)
                     /locus_tag="VGY20_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274263.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6186..7163)
                     /locus_tag="VGY20_001421"
     CDS             complement(6186..7163)
                     /locus_tag="VGY20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274264.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIKSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7276..8904)
                     /locus_tag="VGY20_001422"
     CDS             complement(7276..8904)
                     /locus_tag="VGY20_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4274265.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            9083..9307
                     /locus_tag="VGY20_001423"
     CDS             9083..9307
                     /locus_tag="VGY20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274266.1"
                     /translation="MTYFRLKKELNWLLRSKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFFEVVRAAATFSTAKNICFGLGLLRSQK"
BASE COUNT         3088 a         1806 c         1862 g         2632 t
ORIGIN      
        1 aaaagatggt agttgccatt gacaaagcat tgataaagga gtaaaattaa ctggttaata
       61 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
      121 tcgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
      181 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
      241 gagacctaaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
      301 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatactt
      361 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
      421 aagcgtcaaa acctctgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
      481 aaggcattga ccctgcgaca ctttatgacc cacatacctg gacagatccc gttttagctg
      541 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
      601 gttacactaa aaaggctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
      661 ctcaaaaatt taaaaaggtg cgctcaaaaa cattcgtgac gcagcacacg gcattttctt
      721 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggtatttct ccagagcaag
      781 agccctctcc tcgccaattg aaagaaattc aagactttgt caaagaatac aacgtcaaga
      841 ctatttttgc agaagacaat gtcaatccca aaattgctca tgctattgcg aaatcaacag
      901 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
      961 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
     1021 tagtgaagaa aacatatggt tatatcggct cagttgctgc tattttacta gctactcata
     1081 ttggaagtta ccagcttggt aagcatcata tgggtctagc aacaaaggac aatcagattg
     1141 cctatattga tgatagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
     1201 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt agtcaaaatt actgaccaag
     1261 gttatgtgac ctcacacggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
     1321 cgattattag tgaagagttg ttgatgacgg atcctaatta ccattttaaa caatcagacg
     1381 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
     1441 tcaagccagg tagtaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
     1501 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
     1561 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
     1621 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
     1681 gtaatcacta tcattatatt cctaaaaaag atttgtctcc aagtgagcta gctgctgcac
     1741 aagcctactg gagtcaaaaa caaggtcgag gtgctagatc gtctgattac cgcccgacac
     1801 cagccccagg tcgtaggaaa gccccaattc ctgatgtgac gcctaaccct ggacaaggcc
     1861 atcagccaga taacggtggc tatcatccag cgcctcctag gccaaatgat gcgtcacaaa
     1921 acaaacacca aagagatgag tttaaaggaa aaacctttaa ggaactttta gatcaactac
     1981 accgtcttga tttgaaatac cgtcatgtgg aagaagatgg gttgattttt gaaccgactc
     2041 aagtgatcaa atcaaacgct tttgggtatg tggtgcctca tggagatcat tatcatatta
     2101 tcccaagaag tcagttatca cctcttgaaa tggaattagc agatcggtac ttagccggcc
     2161 aaactgagga caatgactca ggttcagagc actcaaaacc atcagataaa gaagtgacac
     2221 atacctttct tggtcatcgc atcaaagctt acggaaaagg cttagatggt aaaccatatg
     2281 atacgagtga tgcttatgtt tttagtaaag aatccattca ttcagtggat aaatcaggag
     2341 ttacagctaa acacggagat catttccact atataggatt tggagaactt gaacaatatg
     2401 agttggatga ggtcgctaac tgggtgaaag caaaaggtca agctgatgag cttgctgctg
     2461 ctttggatca ggaacaaggc aaagaaaaac cactctttga cactaaaaaa gtgagtcgca
     2521 aagtaacaaa agatggtaaa gtgggctata tgatgccaaa agatggcaag gactatttct
     2581 atgctcgtga tcaacttgat ttgactcaga ttgcctttgc cgaacaagaa ctaatgctta
     2641 aagataagaa gcattaccgt tatgacattg ttgacacagg tattgagcca cgacttgctg
     2701 tagatgtgtc aagtctgccg atgcatgctg gtaatgctac ttacgatact ggaagttcgt
     2761 ttgttatccc tcatattgat catatccatg tcgttccgta ttcatggttg acgcgcgatc
     2821 agattgcaac aatcaagtat gtgatgcaac accccgaagt tcgtccggat gtatggtcta
     2881 agccagggca tgaagagtca ggttcggtca ttccaaatgt tacgcctttt gataaacgtg
     2941 ctggtatgcc aaactggcaa attatccatt ctgctgaaga agttcaaaaa gccctagcag
     3001 aaggtcgttt tgcaacacca gacggctata ttttcgatcc acgagatgtt ttggccaaag
     3061 aaacttttgt atggaaagat ggctccttta gcatcccaag agcagatggc agttcattga
     3121 gaaccattaa taaatctgat ctatcccaag ctgagtggca acaagctcaa gagttattgg
     3181 caaagaaaaa cgctggtgat gctactgata cggataaacc caaagaaaag caacaggcag
     3241 ataagagcaa tgaaaaccaa cagccaagtg aagccagtaa agaagaagaa aaagaatcag
     3301 atgactttat agacagttta ccagactatg gtctagatag agcaacccta gaagatcata
     3361 tcaatcaatt agcacaaaaa gctaatatcg atcctaagta tctcattttc caaccagaag
     3421 gtgtccaatt ttataataaa aatggtgaat tggtaactta tgatatcaag acacttcaac
     3481 aaataaaccc ttaaccaaaa gaagatctca ttattaaagc actgctttat caaagcaagt
     3541 tacggtgatt ttgaagtcat tttatgtaac gagtagtgat aaaagttgga taatagcggt
     3601 tttcttttgc aaagaaatgg tatccatgtt agaataataa aaaaaagagg aggattctaa
     3661 tcatgtcaga agaaaaatta aaatcaaaaa ttgagcaagc atctggtggc ctaaaagaag
     3721 gtgctggtaa gttgacaggt gataaagagc tagaagcaaa aggatttgtt gaaaaaacaa
     3781 ttgctaaagg caaagaactt gcagacgatg caaaagaagc ggttgaaggt gctgtagatg
     3841 ccgttaagga aaaattaaaa taattaaaaa accagctgta agctggtttt ttaatggtgt
     3901 gattttgtta gaaaaagact caataggtaa taaccataga tgttaggtat gcttgaacag
     3961 ttgagtacga ttctcttcaa tcatatagca cgcgtcacaa agtttttcta acatatcaaa
     4021 atcatgacta ataatgatga gacctaaatt tctatccttt acaatctcca acaggctttt
     4081 ccatacacta gcttgtgtaa tgctatccaa catagtagtc atttcatctg caataaggta
     4141 tttcgtctct ggatgtaatg aacgcacaat cgaaaagcgt tgcaattccc ctcccgagag
     4201 ttcactaggg cgacgcttta gccatttttc ttgaatgcca aaagcatctc gcaaatcttg
     4261 acttggatag taggcttctt ccaaactttt tttcataggc cataaaggat tcatggtttg
     4321 ttcaggatgt tgttggataa gttgcacagg tctaaatgct ttgttaggca aatgactgcc
     4381 atcaactaat acttcaccag atttaggctg caaaaaaccg gcaagcactc tggataaact
     4441 cgtttttcca cacccacttt gtccaaatat ccctaagatt tgaccaggtg ctacctctaa
     4501 atcaatctcc ttaaaaagcc attgatcttt tttatgataa aagccaagct ttttagcctc
     4561 taaggtcatg agtaactcct ttcaaaaagt cttgctgagg gagtgtgcgc cataaacgtc
     4621 tcgcaaattc tgtttgtaat tgctctccac ctctgctaaa aaagctagct ggggctgttt
     4681 caatagcttt tccctcttta aaaatagtta tacgatccgc aatttgacta gctgctacaa
     4741 tatcatgagt gataaagatg acgcttattc ctttatctgc aaaagagcgt agttgatcta
     4801 aaaccatttg cagagcatct ggatgtaatc caggggtggg ctcatccgca ataatcaaag
     4861 aaaccgtatc actaatacac gttgtaaaca aaacacgtcg gagcattccg ccagaaagtt
     4921 ggaaaggata caagtcacca tcactttctt ttaatccaaa ctgttgaaac aatccttctt
     4981 gagtagcctt agcattttct gagataccca agcgcacctg atgtttgact ttcatagatg
     5041 gatctaaata attaacggat tgtggaatca acgtcatttc ttttcctcgc aactgtctga
     5101 tgcgtttaga agttagtgat tgaccacgat aaatcatatc tcctgttaca gctgcatttt
     5161 taggaagaat atccataata gcatgtgcta ataaactctt gcctgaacca ctagcaccta
     5221 taatagctaa taactcacct tttttaactt ctaaattcag cgcttgtatc ggtgtggatt
     5281 gaaatggttt taaaaatctt ccgtattgag tgaaggtgat ggagaggtct ttaatgctta
     5341 ataatgtttc tgtcatatct ctacctccta aaaatgatcc gtttgagggt aaaagagttt
     5401 ctttaaagat tctccgatag tatcaaaggc attgacaacc aaaataagat aaaggcctgg
     5461 aaaaatcacc aaccaccaat tgccaagaga gatatgctta gctgcctctg acagaatgat
     5521 accaaccgaa ggttgttcgg cagaaaggcc aaatcctaag aaagtcatgg atgcttcatg
     5581 caagatgacg tgcggaaata agaggataaa cccaatgaaa atttgagaag caatcaaagg
     5641 caggatatga tgcctcacaa tataataagg cgtttttccc atgcttttag agagctggac
     5701 aaaggcttta ttctttagat catagacttc attgcggata agccttgcta gagagggcca
     5761 atgggtaaca gccgttgcaa tgataacccc ttgagcccct ttcccaacaa caaaagaaat
     5821 gagaatcata aaaatcaaat gaggcatacc aataaacaaa tcaaccaacc aggctattat
     5881 tttatcaata aggctatttc ctagacctgc aagcactcca aaaacggtcg caagaaagac
     5941 tcccataagg gcacctaata agccgacttg taaagagaaa taaagccctt taatcgttct
     6001 gacaaacata tccctaccta aaccatctgt tccaaaaaga tggtttaagg aaggagcgag
     6061 gttgcgtaaa gctgcattgg tttccaaagg cgtcctatag aaataaaggt ttaaggctag
     6121 aatactaaga atgagagaaa tggcgatacc tagttgccat aaaaccatcg ttcgacgttt
     6181 caatatcata cttttctcct taactgtgga ttgatgatgc tatttaaaat gtccgcaata
     6241 agattgcccg caaaaacaaa taatgtccct atcatcacaa tagctagaag gagcggtgta
     6301 tcacttttaa ggcctgcttc agtgagggta gaccctaagc caggatatga gaaaacttgc
     6361 tcagcaagaa cagatcctcc aaacaattct ccaaaatagg aaaaatgcag tgtaatagct
     6421 ggtacgatag catttctaag acaatgatgt ttaaaaattt gccactgtgt ttccccacgc
     6481 gctctggcaa ataagacata ttcactggaa agcaccgaca tcattttagt tctcgtatga
     6541 agagtgacat tggcaatgcc tagaatactt agcgtgaaaa caggtaacat aaggtgctta
     6601 atccgatcag ctaacgtaat atcttgactt aaagtgccta tcggagaaga aataccaatc
     6661 gggaaccacc ccagctggac agaaaagatt aataaaaaaa tgagtccaat ccaaaacgtt
     6721 ggtactgata tctgaaggta agaaaaccac ctgacaactc gatcaagtaa tttcccttga
     6781 tggaaagcgg ataacgttcc taagataaat ccaataagac ccgataagat ccaagagagt
     6841 cccataagta tgaaagaagc acctgcccgt gatttaataa tatcactaac aggttgccga
     6901 taaacgagcg aggtccctaa atgtccctgt atcacatttt ttaaccaaat aaaatattgg
     6961 actagagctg gcttatccaa gccatagtgg tgagcaatcg ctttgtactg agcaggggtt
     7021 agtgatgtgt cataattgac acttgccatg actggatcta ctggagattg ttttaacaaa
     7081 acgaaggtca aaacagatac cccaaaaata agcgtgacac atctgatgat tttccagatg
     7141 ataataatgg tagtacgttt cacaatgaaa tctccttaat cgataagaaa aggagccgag
     7201 gaaacgatca gcacagtttt gtgtatcttg ttttctcaga ctccaaactt aaagtcgctg
     7261 tattaactaa acgcgttact tagttgattc atcccaagtc cactcggcaa tgttagtcaa
     7321 taatgaccaa tcatgaccat gactgtggac gccttgttta cctacattga tacgtttatc
     7381 accaatataa gtatggttaa ggctcaccaa ccatacattt ggcaaatctc caagagtaga
     7441 agcacctgtt ttgccatccc actgcgctaa cttccaatat tcgttagctt tgtcaaggtc
     7501 agaagatgtc attgctttgt caaggtactt agtcacggta ggattgttat aaaacgtaat
     7561 attggtccaa cctttacctg ctaggcttgg atgatgtgat tcataaaatt gctgcgcgtg
     7621 atgacgtcct ccggcataaa gtaaggctga gtcatgtgac ttcgttgcca tttcatccca
     7681 gttactagct ttgagtttaa tagtaatccc tagggctttg gcttgctctg ctacttcaac
     7741 ggctaagttc gctcgcaatt gatcattagt agggtagtac agatcaaacg ctgcatcaag
     7801 gtcacctttt ttacggctac cgtctgcttg ttctttccat cccgcttttg tcaacaattg
     7861 cttagcttta gctactttat tatctttaat ggctgttttt ggattccaaa atggtgtttt
     7921 atcaataatt gaataagctg gtttaccata accatttaaa accgtatcga gaactttttg
     7981 tctatttaaa ccaatagtca aggctttgcg gattgctgga tcactagtga catcatttcc
     8041 tacaggataa ccatcaggag aatcagtgat gacgcctttt ttcacataag gtaatgataa
     8101 gccgcgcaca tcatttgatg gaatgtcaag gaggcgagtg cctttgactt tcttactagc
     8161 aagttctggt gtcgcgtaga tcatatcaac atcaccagat tctaaagcag ctagtgctgt
     8221 gttttcatca agtaagaccc aagtccactt tttaaagtat ggttttttcc catgccaata
     8281 agggttacga acaaaaatag cttgttctcc agccttatat tcttttacca tgtaaggtcc
     8341 tgaaccgata ggattgctct tatacttatc attgtaatgt tttttaggga cgattgggat
     8401 ttcagtcaac tgtgctgtaa atgtcgaatg cgcctcagtc aaatggatat tgacctgatt
     8461 tttcccaact acttcaacgt tcttgatgaa ggttagatcc caagcctttc catctgcttt
     8521 caacatatca taagtaaact taacatcatc agcagtaaca ggctcaccat ttgagaattt
     8581 aaaatcatca tgcaagtcaa acgaccaagt cagcccatct tcagagagat ggtatgtttt
     8641 agcaagctct ccttttatat ctagttcagg agaacgtttc aatagagtgc tatgagtgat
     8701 attcccttca ttgtggactc cataacggtc ctttggatcg aattcatgag ggagctttgc
     8761 ccccatagaa acgacaattt cgtcttttgg acgttgtttg cgctgacgtt cttttgtttg
     8821 aggcttttgt tgttgacatg caactaaaat aagcccagtc aaaaataacg tgataataga
     8881 gaagtatttt aggtattttg acacaatcaa atctccttat ttctaattaa taaataataa
     8941 tagtaacgac tccatctcat tatagcaata aacaaatagg agggcaattg tttaactggt
     9001 taattagaat ttttgttatt ttcaggaaga tactcagggc ttttttataa gttgatgtat
     9061 taacaaaaga taaagaggtt tcatgactta ttttagatta aaaaaagaat taaactggtt
     9121 actaagaagt aaaggaattg tagtttacga agggaatcaa aaacttcaga tagctattaa
     9181 tacaggtaaa aataacagtg aaaaagtctt ttttgatact ttttttgagg tggttcgggc
     9241 ggcagcgact ttctccacag caaaaaacat ctgttttggt cttggcttac ttcgttcgca
     9301 gaaataggaa atcgggtatc acattagcag agtagttcat gctttttgag catcacactg
     9361 ggtctgtcta cgttctaaaa gcttttgt
//
LOCUS       DAUCWO010000062         9281 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUCWO010000062 DAUCWO010000000
VERSION     DAUCWO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.062"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            237..2237
                     /gene="metG"
                     /locus_tag="VGY20_001424"
     CDS             237..2237
                     /gene="metG"
                     /locus_tag="VGY20_001424"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ4274267.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGAVPAYVDNGT
                     AFDADLSQLIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETTPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLEPVSDLSVLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKSYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLMVLTVDSSVPNGSIIG"
     gene            2732..3745
                     /gene="nrdF"
                     /locus_tag="VGY20_001425"
     CDS             2732..3745
                     /gene="nrdF"
                     /locus_tag="VGY20_001425"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ4274268.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            3749..4237
                     /gene="nrdI"
                     /locus_tag="VGY20_001426"
     CDS             3749..4237
                     /gene="nrdI"
                     /locus_tag="VGY20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ4274269.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            4204..6384
                     /gene="nrdE"
                     /locus_tag="VGY20_001427"
     CDS             4204..6384
                     /gene="nrdE"
                     /locus_tag="VGY20_001427"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ4274270.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQAFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQMTTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(6545..7297)
                     /locus_tag="VGY20_001428"
     CDS             complement(6545..7297)
                     /locus_tag="VGY20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ4274271.1"
                     /translation="MLKKRYQLAIVLLLSCFSLIWQTGGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAATLIKKRWGKGLVYPRAEQEAMAAYTCQQAGPINTSLDKAKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
     gene            7756..8208
                     /locus_tag="VGY20_001429"
     CDS             7756..8208
                     /locus_tag="VGY20_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274272.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMARPVKAEVSRSEGAERTEQ
                     QNLFRGPQEDFEEGNDEAGSYTYTWEKDRDFGKQQGLEAGKNGYGPNISRDDISVPVG
                     MQNPDEYKDGYQEGYSEGWHQEHPLLGVVFDVFVSIWGVVSGLFSEAN"
     gene            8741..9139
                     /locus_tag="VGY20_001430"
     CDS             8741..9139
                     /locus_tag="VGY20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274273.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMTQPVKAEIVKSPEAEQTVM
                     QKNGVTETDNSYQKEWDRGYDEGYKKGNERNSPKKPEHSPVADGSGYSDGYSTGYGAG
                     WYDGHLVEAIIDGLISWFNYIFFGSGGSSE"
BASE COUNT         2749 a         1992 c         2004 g         2536 t
ORIGIN      
        1 gggcacaaaa ggatgaagag aaagttttca tgcgtacggc gtgccctcct ttgattgact
       61 ggttttgctg ccccttaaaa tatgatataa tagactattg atagctgtta cttaacagaa
      121 ggctgataaa aaaggttcca ttagccttga gggttgaatt taaacaggaa aaaacgaaat
      181 cctacactat gcatctcaat catactttca tttgagtatg gttctgaaag gaaactatga
      241 tgaaaaaacc attttacatt acgacaccca tttattaccc atctgggaaa ttacacattg
      301 gttctgccta tacaactatt gcttgtgatg tcttggcacg ctacaagcgc ttgatgggcc
      361 atgaggtctt ttacctcaca ggacttgatg agcacggaca aaagattcaa accaaggcaa
      421 aagaagctgg tattacacct caaacctatg ttgataacat ggcaaaggat gtcaaagccc
      481 tttggcaatt gctagatatt tcctatgaca catttatccg gacaacggac gattaccacg
      541 aagaggtcgt tgctgctgtc tttgaaaaat tgctggcgca agacgacatt taccttggcg
      601 aatactctgg ttggtattct gtctctgatg aggaattttt cactgaaagc caattaaaag
      661 aagtctttag agatgaagac ggtcaggtta ttggaggaat tgccccatca ggtcatgagg
      721 tcgagtgggt ctctgaagaa tcctatttcc tacgccttag caaatacgct gatcgtcttg
      781 tggccttctt taaagagcga cctgacttta tccaaccaga cggtcgtatg aatgaaatgg
      841 tgaaaaactt tatcgagcca ggtctagaag atttggctgt tagccgtact acctttacat
      901 ggggtgtacc tgttccatca gacccaaaac acgtggttta tgtctggatt gatgccctcc
      961 taaactatgc gacagctctt ggttatggtc aagctaatca cgctaacttt gataaattct
     1021 ggaatggaac ggtttttcac atggttggaa aagatatctt gcgtttccac agtatctact
     1081 ggccaatctt actcatgatg cttgatttac caatgccaga ccgcttaatt gctcatggtt
     1141 ggtttgtcat gaaagacggt aaaatgtcta aatcaaaagg caatgtggtc tatccagaaa
     1201 tgttagtaga gcgctttgga ctagatcctc ttcgttatta cctcatgcgt tctttgccag
     1261 tgggttcaga cgggaccttt acccctgaag attatgtggg tcgtatcaac tatgagttag
     1321 ccaacgacct tggcaattta ttgaaccgta ccgttgcgat gatcaacaag tattttgacg
     1381 gcgcggtgcc agcttatgtt gacaatggaa ctgcctttga tgctgactta agtcaactga
     1441 ttgatgctca gctagcagac taccataagc acatggaagc tgtagactac ccacgcgcct
     1501 tggaggctgt ttggactatc attgctcgta ccaacaaata catcgatgaa accactcctt
     1561 gggtgctcgc taaagaagat ggtgacaaag ctcaactagc cagtgtcatg gcacacttag
     1621 cagctagctt gcgcgtggtc gctcatgtga tccaaccctt tatgatggaa acatcagctg
     1681 ctatcatggc ccagcttggt ttggaacctg tttctgattt gtcagttctt gctttagcag
     1741 attttcctgc caatacaaag gtcgttgcca aaggcacacc gattttccca cgtcttgata
     1801 tggaagctga gattgactac atcaaagccc aaatgggtga cagctcagct atttcccaag
     1861 aaaaagaatg ggtccctgaa gaggttgcgc tcaaatctga aaaagacgtc atcacatttg
     1921 aaacctttga tgctgtggaa atccgtgtgg cagaagtcaa ggaagtgtca aaagtggaag
     1981 gttcagaaaa attgctgcgc tttagagtag atgcaggaga cgggcaagac cgtcaaatct
     2041 tgtcaggtat tgccaaatcc taccccaacg aacaagaatt agtgggtaaa aaactacaaa
     2101 ttgttgctaa cctgaaaccg cgtaaaatga tgaaaaaata catcagccaa ggcatgattt
     2161 tatcagcaga gcatggagat cagctaatgg tcttaaccgt tgattcctct gttccaaatg
     2221 gaagtatcat tggctgatgc tgacttatta ttcaaaaaac ctaagcaaga acatgtgctt
     2281 aggttttttt gattgccttc aagaaagacg aataacgaga tgggtgatat ggcagcaaaa
     2341 gcataactat cctaggacct gtcctagtaa gattgctgtg cttttgacaa gtgaacagag
     2401 aggtcttaaa actatatctt gtcataaaaa agattgcaaa acactatatc ttgtgttatt
     2461 atggttttta taaaagttaa gcacgcttaa ctttagccaa acaaaagagg acactagagc
     2521 gtgtcccaca agacagttga gcccttcgct tttttagtag agtaggagtg ctcaagtcaa
     2581 aaagagacat cccatcaaac cattgctgtt tagcaggtct ttttgtgttg ctcaaaaaaa
     2641 caattcctgt ctgtttggcg tctagtgatg acttaggctt agcgctacta gacttttgtt
     2701 tgaaaaatac ctcaagcctt gaaggagaca aatgacccaa cattattatg aacgttcgca
     2761 atccccaatt gaatacgccc tatctgaaac acaaaaacag ttgcgctcca tcaattggaa
     2821 ttaccttaat gatgacaaag atttggaagt gtggaaccgt gtcactcaaa atttttggtt
     2881 gcctgaaaag gttcctgttt ctaatgattt aaattcttgg cgctccttgg gtgaggattg
     2941 gcaacagctc atcacaagga cctacactgg gttgacctta ctagataccg tacaagcaac
     3001 ggttggtgat gtggcacaaa ttcagcattc acaaaccgac cacgaacaag tcatctatac
     3061 gaactttgcc tttatggtgg ggattcatgc ccgctcttac gggacgattt tttcgaccct
     3121 ttgcagcagc gagcaaattg aagaagcaca tgaatgggtg gtctcaacac agagcttgca
     3181 agaccgtgcc cgtgtcttga ttccttacta tacgggtgat gaccctttga aatcaaaagt
     3241 agcagcagct atgatgccag gatttttgct gtatggcggc ttttaccttc ctttttacct
     3301 atcagcacgt ggtaaaatgc caaacacatc tgatatcatc cgcctgattt tacgcgacaa
     3361 agttattcac aactattaca gcggttacaa ataccaacaa aaagtagcac gtcttagccc
     3421 agaaaaacag gctgaaatga aggcctttgt ctttgacttg ctctatgaac tcatcgacct
     3481 tgaaaaagct tacttgagag aactgtatgc aggctttgat ttggcagaag atgctatccg
     3541 ctttagcctt tataacgctg gaaaattcct tcaaaatttg ggatatgagt cgcctttcac
     3601 agatgaagaa acgcgcgttt caccagaagt ttttgctcag ctatcagcgc gtgcagacga
     3661 aaaccatgac tttttctcag gcaatggctc atcttatgtg atggggatca ccgaagaaac
     3721 cacagatgac gattgggagt tttaagaaat ggcagagctg attattgtgt atttttcatc
     3781 taagtccaat aacacccatc gcttcgtcca aaaattaggc ctgcctgctc agcgcatacc
     3841 agtagataat aggccacttg aagtttcaac ccattacctt ttgattgtgc ctacctatgc
     3901 agctggtggc tcagatgcca aaggagccgt ccctaaacaa gtgattcgtt ttttgaataa
     3961 tcctaacaat cgtaaacatt gtaagggagt gatctcttca ggaaatacca attttggcga
     4021 tacctttgcc ttagcaggcc ctatcatttc tcaaaaattg caggtcccct tattacacca
     4081 atttgaacta ttaggcactg ctacagatgt caaaaaggta caggcgattt ttgcccgctt
     4141 aaagcatcac acacacgaca agcaaaaaca aatcaacaac ctgattacag aaaggacaca
     4201 cccatgtcac aaaccaatgc gtcatacctc tcattaaatg cactgacccg ttttaaaaaa
     4261 ccagacggca gctaccattt tgatagcgat aaagaagctg ttaggcgcta cctggaagaa
     4321 catgtctctc ccaatcaaat ggcttttaac tcactagaag ataagctagc ttacctcatc
     4381 aatgaagggt attatgagca ggccattttt gatgcctacc ctaacgacct tatcaaggag
     4441 gcctttcatt atgcctatca acaaggctat cgctttttaa atctcatggg cgctatgaaa
     4501 ttttaccagt cctatgcctt aaaaaccttg gacggtaagc agtatctaga aacatttgaa
     4561 gaccgtgctg tgatgaatgc actcttttta gctgatggag atcaagcttt tgtctttgac
     4621 gtgattgatg ccatcttgca ccgacgcttt caaccagcta ccccaacgtt tttaaatgcc
     4681 ggaaaaaaac gccgtggcga atacatttcg tgctatcttc ttcgtgtcga agacaatatg
     4741 gaatctatct ctcgcgccat cagcaccagc ttgcaactct caaaaagagg aggcggagtc
     4801 gccttgtgtc tcaccaacct cagagaaatc ggagcaccga taaaagggat tgaaaatcag
     4861 gcaacaggca tcgttcctgt catgaaacta ctagaggact ctttttccta tgccaatcaa
     4921 ctaggtcagc gccaaggagc aggtgcggtt taccttcatg cccatcatcc tgaggtcttg
     4981 acctttttag acaccaaacg tgaaaatgcc gatgagaaaa ttcgcattaa gtccctagca
     5041 ttgggacttg tgatcccaga tattaccttc cagttagcca aagaaaacaa agacatggct
     5101 ctcttttcac cctatgatat caagcgtgcc tatggcaagg acatgtcaga catctccatc
     5161 acagaggaat acgataaact gctagccaat ccagccatca aaaagaccta catcagtgct
     5221 agaaagctct ttcagttaat tgcagagcta cattttgagt caggctaccc ttacctcttg
     5281 tttgatgaca cggtcaataa gcgcaaccct catgccaaaa aaggcagaat cgtcatgtct
     5341 aacctgtgtt ctgaaatcgc tcaagtcagc accccaagca ctttcaaaga agatttgagt
     5401 tttgaaacca ttggagagga tatctgctgt aatttagggt ctatcaatat tgcccaggct
     5461 atggcagatg cgccacactt tgaacagctc atcacaacga gcatcagagc acttgatcgt
     5521 gtctctagag tgtctgacct gaattgtgcg ccttctgttg agacaggcaa tgcagccaac
     5581 catgcagtag gattaggcgc catgaaccta catgggtttt tggcaaccaa ccacatctac
     5641 tatgatacaa aagaagccgt tgactttaca gatctgtttt tccatgccat ggcctattat
     5701 gcttttaaag cctcttgcca attagccaaa gaaaaaggcg cttttgcagg attttcgtta
     5761 tcaacctatg cagatggcac ttattttgcc aagtatttgc aagaagatgc caagccacaa
     5821 acagccaagg ttgccacatt gttgcaagat tacggcttca cattgccaac ggtggcggac
     5881 tggcaagccc tagtagcaga tatcaagcag tttggtctag ccaatgcgca cttgctagcg
     5941 gttgctccga caggctccat cagctactta tcatcctgca ctccgagtct acaaccagtc
     6001 gttgctcctg ttgaggtccg aaaagaaggt agcctgggca ggatttacgt ccctgcctat
     6061 cagattgacc aggctaacta tgcttactat gaacgtggtg cctatgaggt cggcccaaaa
     6121 gccatcatcg acgttgtcgc tgctgcccaa aagcacgttg accaagccat ctcattgacc
     6181 ctgtttatga ctgatcaaat gaccacacgt gaccttaacc gctcctatat ccaagctttt
     6241 aagcaaaact gtgcatcgat ctattatgtg cgagtgagac aagacgtcct agcaggcagc
     6301 gaacagtatg acgaggatag tcttgtgaca gctcctggag ctagtgatga gaccaccaca
     6361 gagtgccaaa gctgtatgat ctaacagcta ctagaaagct ctcgtgtccc ctatcacatg
     6421 cataggatca gtgcgctcga ccattcaaga caagcaagca tcagctcttg cttgtctttt
     6481 tttgagcatc agaacgtcaa aaaaaggaca tggagtcctt ttttggtgat cggtgttgag
     6541 gccgtcacaa actgcccttg aaatacgctt ctatgtggag ctttttttgg tcctgtgaca
     6601 cgtaagctcc aacgacctcc agctgacagc ctcttggaaa caagagctca acctctgaag
     6661 gcacagccga ataaggctcg acaaaggctg ccttggcccc ttttttgaca cagatgcgca
     6721 cctccaccgg tcgatgggtc atggcgccat ttttcaaggc tgtcgtgctc ataaaactgt
     6781 gcttggtgta gcgtgtgcca agcttgatct tacaaaggat atgagggtca aactgatggt
     6841 tacggtagta agacgtgaga tcagcgtgtg aaacaccgat atcacgcaaa aacgtctcgt
     6901 atacatagcg gtatactaca atgttccacg ggatgactag ccggtgagtc gcagcatcga
     6961 gctgggccac ctgatccctt agctcaggcg tgagttggct gagctcacct ttggccttgt
     7021 ctaggctggt gttgataggg cctgcctgct ggcaggtata agcagccatc gcctcttgct
     7081 cagcccttgg gtagacgaga cccttgcccc atcgcttttt aatcagtgtc gcagctccct
     7141 tttgatccga aaaggtaaaa taagcaggcg tcccctcaca aaccgcccgc tcatagtgct
     7201 cacagacaaa aagctcgacc aagcccccag tttgccagat cagactaaaa cagctaagaa
     7261 gaaggacaat agcgagttga tagcgctttt ttagcatagt tccccctgac accagcccaa
     7321 aaggctgacc cttcccctag accttttggc tagctcttga caccaccagt ttctcagaca
     7381 ggctaatctt tttgtcatct ctttctccct aaacagactc tcttatcatg atattatgct
     7441 attatgagcg atttgtcaat ccatagtaac cgttttttag ttagataagt aagcttttga
     7501 cctagaggca gacaagagtg gcagtacctg ccgtttgctg tggtggctaa taaggactaa
     7561 cataaaatct caaacgcttt caaaaatagc tcctgagctt aaaaaaggcg aattaccaga
     7621 tttgttgagc ttaagggaga ttgccatgat tttctaatca tttgatggtt aaaatgataa
     7681 aaacatgaac ttgaagtgaa agaatgcgag tagtaaacta gaaccgtatc aaaaataatg
     7741 aaatggagtg attttatgaa aacaaaatca aaacgctttt taaacctagc aaccctttgc
     7801 ttggccctac taggaacaac tttgttaatg gcacgtcccg taaaggcgga ggtgtcaaga
     7861 agcgaaggcg ctgagcgtac agagcagcaa aatcttttta gaggtcctca agaagatttt
     7921 gaagaaggta acgatgaagc cggaagttat acttatacat gggagaaaga tagagatttt
     7981 ggaaaacaac aaggacttga ggctggtaaa aatggttatg gaccgaatat aagtcgggat
     8041 gacatatcag ttcctgtagg tatgcaaaat ccagatgaat acaaagatgg gtatcaagaa
     8101 ggttatagtg aggggtggca tcaggagcat ccacttctag gagttgtatt cgacgttttt
     8161 gtttcgatct ggggcgtagt gagtggtctt ttcagtgaag caaattagtt atagttacgc
     8221 tgttagagag tagtattatc tgttgaaagt actgtaatgt ttaggctaca gcgatatttt
     8281 tggtacatat agctaaaagt actacagtta attttttatc acaagcattt cccacactaa
     8341 tctgctataa ttgggatagt ctctttagta gtcaggccac ctctgatagg ggtggctttt
     8401 ttgggtttta gtgacccgtg tgttgagaaa ggagcatgta cgtcagaaaa gaaagtgttg
     8461 cccgttctaa tatgaaccga cagttattcc ttattaaata gacgcaaata tccacgcttt
     8521 cactgtggtg gttgataaga actaacacaa aatctcaaac gctttcaaaa acaactccta
     8581 agcttaaaaa aagctgatga acagattttt tgaacttaag agagattgcc atgattttct
     8641 aatcatttga tgtttaaaat gataaaaata tgaacttgaa atgaaagaat gtgagtagta
     8701 aactagaacc gtatcaaaaa taatgaaatg gagtgatttt atgaaaacaa aatcaaaacg
     8761 ctttttaaac ctagcaaccc tttgcttggc cctactagga acaactttgt taatgacaca
     8821 gccggtaaag gcggagatag taaaaagccc agaagcggaa cagactgtaa tgcagaaaaa
     8881 tggagtcact gagaccgata atagctatca gaaagaatgg gataggggtt acgatgaagg
     8941 atataaaaag gggaatgagc gtaattctcc aaaaaaacca gaacattcac ctgttgctga
     9001 tggtagtggt tatagtgatg gttatagtac aggttatgga gctggatggt acgatggtca
     9061 tttagtggaa gctattatag acggtttaat atcgtggttt aattatattt tctttggatc
     9121 aggcggttca tccgagtagt catgtctatc tagctatgct tttcggtaag taactagaaa
     9181 ataagaacgc taaagacgaa tctgaagttt tatcgtaatt ctataggtgt gactaccgta
     9241 actatacaat actgtatgag gagctatttc agaatatttt t
//
LOCUS       DAUCWO010000063         8754 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUCWO010000063 DAUCWO010000000
VERSION     DAUCWO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.063"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            673..909
                     /locus_tag="VGY20_001431"
     CDS             673..909
                     /locus_tag="VGY20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ4274274.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            902..1600
                     /locus_tag="VGY20_001432"
     CDS             902..1600
                     /locus_tag="VGY20_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ4274275.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDIVCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLADWVARETPIGRWTKPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            1676..2635
                     /locus_tag="VGY20_001433"
     CDS             1676..2635
                     /locus_tag="VGY20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ4274276.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVIIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANMDKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQIELVIYGQKPQVINHNKH
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            2968..4305
                     /locus_tag="VGY20_001434"
     CDS             2968..4305
                     /locus_tag="VGY20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4274277.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            4478..5860
                     /gene="glmU"
                     /locus_tag="VGY20_001435"
     CDS             4478..5860
                     /gene="glmU"
                     /locus_tag="VGY20_001435"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ4274278.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKAVTVIGHKSEKVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            5891..6445
                     /locus_tag="VGY20_001436"
     CDS             5891..6445
                     /locus_tag="VGY20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4274279.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            6445..6696
                     /locus_tag="VGY20_001437"
     CDS             6445..6696
                     /locus_tag="VGY20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274280.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            6715..7410
                     /locus_tag="VGY20_001438"
     CDS             6715..7410
                     /locus_tag="VGY20_001438"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ4274281.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENSPV"
     gene            7561..7902
                     /locus_tag="VGY20_001439"
     CDS             7561..7902
                     /locus_tag="VGY20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274282.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTD
                     EKSHLQYSKDNAKLQLRNILNGYQSDLGRNYSSYYYYNLRTVMGLSSEQEIEKYYEEL
                     KNKLHDMYNHY"
     gene            complement(8001..8648)
                     /locus_tag="VGY20_001440"
     CDS             complement(8001..8648)
                     /locus_tag="VGY20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ4274283.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLETHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
BASE COUNT         2593 a         1539 c         2006 g         2616 t
ORIGIN      
        1 ttttatagaa tttcagaaag attttataga attattatta cggtttttcg attttggaaa
       61 ataaaaggta ccttcaaggt gacttttatt atttaagtgg cttaatgaat tggttaaaat
      121 ctttttagtt tagtaaaatt gctatttaag gttagccacc tctcgtatgg tcacagttga
      181 tgaattatgc tatagtatag acataaaaaa tagttcaaat tccttcttgc ctgttggtgg
      241 attttgaact gaaagcctct ctgattgcag ttggtaggct tttttgatgc tttttcgtac
      301 tttttttatt tattacgcta aaaaatgtta tacttgatga agaaaaagga gagtttagaa
      361 cgttaagata cctcacagca gattgataga ggatatggaa gaaggttagc tttcccaaga
      421 gatgccgttt gggaatggta taacaataac tggcagaagt tataagtaaa acaaaaacaa
      481 attaactcag atgattttta gacgttccca cgcgtgtgcg aatgcgtgtg cgaattgatg
      541 ttatcaataa atcctttaat aacgcctttt tcgaacagga aacaaattta ttatcccacg
      601 cgtgtgcgaa tgtgtgggcg aattaattgg agaaaaaatg agaaaatacg atatacaaaa
      661 aaggagaaca atatgacatt tgaagagatt ttaccaggtt taaaagccaa gaaaaaatat
      721 gtacgtacag gttggggtgg cgctgaaaat tatgtgcagt tgtttgatac ccttgaagtg
      781 gatgggaaag tactacaggc gacgccttat tttctgattc atgttactgg tgcaggtgaa
      841 ggtttttcca tgtgggcacc gacaccatgt gatgtgttag cagaggattg gatcgaggtc
      901 aatgactaaa gttgttttag tgacgggctg tgctagtggg attggttatg cacaggcccg
      961 ctattttttg agacaagggt atcacgtata tggtgtggat aaatcggata aaccggactt
     1021 aaacggtaat tttcacttta tcaaattgga tttatcctct gagttatccc cactatttaa
     1081 agtggtcccc agtgttgata ttgtgtgcaa tactgcaggg attttagatg cctacaagcc
     1141 acttttagac gtcagtgatg aagaagtgga acacctattt gatattaatt tttttgcgac
     1201 ggtgaagtta acacgtcatt atcttagacg tatggtggaa aagcagtcag gtgttatcat
     1261 caatatgtgc tcaatagcaa gttttatagc gggtggcggt ggtgttgcct atacgagttc
     1321 taagcatgcc cttgcgggat tcacgcgcca gctggctttg gattacgcca aagatcagat
     1381 tcacattttt ggtatagcac caggtgctgt caaaaccgct atgacggcta gcgattttga
     1441 acctggtggc ttagcagact gggtagctcg tgagacgcca attgggcgtt ggactaaacc
     1501 agatgaagtt gctgaattaa cgggatttct tgcgtcagga aaagcgagat caatgcaagg
     1561 tgaaattgtc aaaattgacg gtggctggac tttgaaatga tcacatgcct tagcttgagc
     1621 tggcgcagta agccctagag gtgtgacgag tcaaacagtc aaaggagatg aacaaatgaa
     1681 attagcggtt ttggggacag gtatgattgt taaggaagtt ttgcctgtct tgcagaaaat
     1741 tgatggtatt gatctagttg ctatcttatc aactgttaga agtttaaaga cagctaaaga
     1801 cctcgctaag gcatatcaca tgcctctagc tacgagtgaa tatgaggctg tcttaggcaa
     1861 tgaggagatt gacacggtct atattggact gccaaatcac ctgcattttg cctatgccaa
     1921 ggaggctctt ctggctggta aacatgtcat ttgtgaaaag ccctttacca tgacagcagg
     1981 agaattagat gaactggtga taatcgctaa aaaacgaaaa ctcattttgt tagaagcgat
     2041 tacgaatcag tatttaagca acatgacctt tatcaaagag catcttgacc aacttggcga
     2101 tatcaaaatt gtagagtgta attattcaca gtattcttca cgctacgatg ccttcaaacg
     2161 aggtgatatc gcaccagcct tcaatcctaa aatgggtggt ggagctttac gtgatttaaa
     2221 catttacaac attcattttg tggtaggatt atttggtcgc cctaaaacgg ttcaatattt
     2281 agccaatatg gacaagggca ttgacacctc aggcatgctt gtcatggact atgagcagtt
     2341 taaagtggtg tgtatcgggg ccaaagactg cactgcggaa atcaaatcaa ccatccaagg
     2401 taataaaggg tcactggcag tgcttggagc aacaaatacc cttcctcaga ttgagctcgt
     2461 tatttatggt caaaaaccac aagtgattaa ccacaacaag catgaccacc ggatgtatga
     2521 agagtttgtc gcttttaggg acatgattga ccaaagagac tttgagaaag tgaatcaggc
     2581 tttggaacat agccgagctg ttatggctgt gttggagcgg gctgttcatt cgtgattgta
     2641 atctggtagc cattgccttt tgtgcagctg ctatggtaat ggttgaaaga cgtgttttca
     2701 cgagggttaa ccccctcgtg cttttttggt aaaaataact tgttcaggat ttgggctttc
     2761 tttgtgagat gagagatgcc tgacgaattg atttgattaa aataatgata aagccaaacg
     2821 atttaagaaa tgaccgttcg gaaattctgt cccaaaggag ctctagaagc agtaaaatcg
     2881 aatttgtaag cgttgacact gttgcataac tctgttacac taagagaatg tcatgacaaa
     2941 aattacagtg taggagttta attgtttatg gaagaggatt ataacaaaag agaacctgag
     3001 aagtttacgc aatttctgcg ccgtcaaaag gttgtctttt ttgtcgcatt ttttggttat
     3061 gtgtgtgctt acttggtacg caacaatttc aaactaatgt caaacactat tatggtccaa
     3121 aacggttggg acaaggctca aattgcgacc ttgttatcgt gtttgaccgt ttcttatggc
     3181 ttagcaaaat tctatatggg ggccttaggg gatcgtgtta gcttgagaaa gctcttttcg
     3241 atcagcttgg gtgccagcgc tttaatttgt attttaatcg gttttttcaa tagttcaatg
     3301 gtggtattag gaatactgct agtcttgtgt ggtgtcgtac aaggtgcctt ggcacctgcc
     3361 tcacaagcca tgattgccaa ttattttcca aataaaacac gcggcggagc catcgctgga
     3421 tggaacattt ctcaaaacat gggatcagcc ctcttgccat taaccatcgc cttgctcacc
     3481 agtatgggct tggtggtacc agctaatggc aatattttac ttgccttttt aatccctggt
     3541 gttttagtgt tcttgtttgc tttgtgttgc tggaagcttg gcggtgataa ccctgagtct
     3601 gagggacttg actctcttcg aacgatgtat ggtgatgcgg gcgagtcagc tgttgctagt
     3661 gaagaagaaa agcataacct gtcttattgg caactcatct ggaaatacgt tttttgtaac
     3721 ccgtcgctct tacttgttgc tgctgtcaat gtggcccttt attttgttcg ttttgggatt
     3781 gaagactgga tgccgatcta cttgtcacaa gtagccaata tgtcagaggc tcatattcat
     3841 tttgcgattt caatgttaga gtgggtagct attccaggct cgctggtatt tgcgtggtta
     3901 gcggttcgtt atcctaataa aatggccaag attggggcta ttgggctttt tgtgttagcg
     3961 gctattgtct ttgtctatga acgcttgact gccacaggtg ctccaaacta tttcttgttg
     4021 cttgttattg caggtatttt agggtcattg atttatggcc cacagttgat cgtgaatatt
     4081 ttaacaatta actttgttcc tttaaatgtt gcaggaacag cgattggttt tgtaggagta
     4141 acagcttatc tcatcggcaa tatgggagca aactggctga tgccgatttt ggcagatggt
     4201 tttggctggt tttggtcata tattgtcgtt gcagccttat ctgctttttc agcggttggt
     4261 tatttgattt tagccaaacg tgaggaagaa atcatcaaag attagctagg caaatgccta
     4321 agaaagacag acttattgct gaagtcctgt cttttttagt catcacaagc cgcaaatgac
     4381 actataaaag acaagaaaat gctaggatgt ttcaggaaaa aaagagaaaa tatgctataa
     4441 tgagaaggta tttaaattat atagtaagga aaaacaaatg acaaattatg ccattatttt
     4501 agcagcgggc aaagggactc gcatgacttc tgatctccca aaagttcttc ataaagtatc
     4561 tggactaacc atgcttgagc atgtttttcg tagtgtaaaa gcgattagtc ctgaaaaagc
     4621 cgtgacggtt attggtcaca aatcagaaaa ggtacgtgct gtcttagcag atcaatcagc
     4681 ctttgtccat caaacagagc agttaggaac tggccatgcc gtgatgatgg cagaaacaca
     4741 actagaagga ctagaagggc ataccttggt gattgcagga gatactccct tgatcactgg
     4801 agaaagcctg aaaagtctga ttgattttca tgtgaatcat aaaaatgtcg ccactatttt
     4861 aacagctacg gctcaagatc catttggtta tggtcgtatt gttcgcaaca aagatggcga
     4921 agtgatcaag attgttgagc aaaaagacgc caatgagtat gagcaacaat taaaagaaat
     4981 caatacagga acctatgtgt ttgataacaa gcgtctcttt gaagcgctca aatgcatcac
     5041 taccaacaat gctcaaggag agtattacct gactgatgtt gtggctattt ttagagcaaa
     5101 caaagaaaag gtaggagcct atatcctgag agacttcaat gaaagtcttg gtgttaatga
     5161 tcgtgtggcc ttagcaacag ctgaaacagt gatgcgccaa cgcatcaccc aaaaacatat
     5221 ggtcaatggg gtgactttcc aaaatcctga aaccgtttat attgaaagcg atgttgagat
     5281 tgctccagac gtccttatcg aagggaatgt gaccttgaaa gggcgtacac atatcggctc
     5341 aggaactgtc ttgacgaatg gtacttatat tgtggattct gaaattggtc aaggaagtat
     5401 catcaccaac tcaatgatcg aatcatctgt tttagctgca ggagttacgg taggccctta
     5461 cgcccacctt cgaccaggaa cgaccttaga tagggaagtc catatcggta actttgttga
     5521 ggtcaagggt tctcacattg gtgaaaaaac aaaggctggg catctgactt atattggaaa
     5581 cgctcaggtt gggtcttcag tcaatgttgg ggctggaacc atcactgtca actacgacgg
     5641 ccaaaacaaa tatgaaacag tcattggaga tcacgctttt attgggagca actcgactct
     5701 cattgcacct ttggaagttg gagataatgc tttaactgca gctggctcaa cgatttctaa
     5761 gacagtacca gctgatagca ttgttattgg tcgtagccgc caagtcacaa aagaaggtta
     5821 tgccaagcgc ctagcgcatc acccaagtcg aagcaaataa ttcaagatct atttagtaag
     5881 gaggccaggc atgaaatttg aagaaaaaac gcttaagaga caaacagttt ttgatggtca
     5941 tatttttaaa gttgttgtgg atgatgttga acttcccaac aatctgggac aatccaagcg
     6001 agaattaatt tttcatcgtg gtgccgttgc tgttttggct attactcctg aacgtaagat
     6061 tgtccttgtc aaacaatacc gtaaggctat tgaaagtgtc tcatacgaga tcccagcggg
     6121 taagctagag attggtgagg aaggctcaaa gctaaaagct gctgcacgtg agctagaaga
     6181 agaaacagcc tatacgggga cattgacgtt tttgtatgaa ttttatacag cgattggttt
     6241 ttgcaacgaa aaaatcacac ttttcttagc gacagaccta acacaagttg ccaatcccaa
     6301 acctcaagac gacgatgaag ttatcgaggt cttagagttg acatatcaag agtgcatgga
     6361 cttagtcgct caaggtaaac ttgctgatgc taaaacgttg attgctttgc aatactatgc
     6421 cttacatttt ggaggtgagc agtgatggga aggccattgt tgaccgatga tatcatcgaa
     6481 aaagcgagac gaatggaaac atttgagcca gatgatgctg tagactttga cacaaaagta
     6541 atgacgctac cagaaaaaga tgaaaaagca cgcatttata aaagccgtcg gattgaaaat
     6601 gccaaacgca gccaattgca atctaaatta aatgtgattt tgatagctgt tatgctgtta
     6661 attgccatct tggtatatgc tatcttttat ctttaaaaaa cgtaaggaaa tgatatgaaa
     6721 ataggaatta ttgctgctat ggaggaggag ttgtctctgc tcttagcaaa tctcttggat
     6781 gctcaagaac atcaggtgct ttcaaaaact tactatacgg gtcgttttgg caagcatgag
     6841 ctcattttgg tacagtcagg tgttggaaaa gtgatgtctg ctatgacagt tgcgatcttg
     6901 gttgagcatt tcaaagccca agctattatc aatacaggat cagcaggggc agtcgcttca
     6961 catttagcga ttggagatgt ggtggttgca gatcggttag tgtatcatga tgtggatgct
     7021 actgcatttg gttatgctta tggtcaaatg gctggtcaac cattatacta tgattgtgat
     7081 ccacagtttg tggctatctt caaacaggtc ctaaaacacg aaaagacaaa tggccaagtt
     7141 ggtttgattg caacaggaga cagttttgtt gctggtcagg acaagattga ccagatcaaa
     7201 acagctttta gcgacgtgtt agcagtagaa atggaaggcg cagctattgc tcaagcagca
     7261 catactgcag ggaaaccctt catcgtcgtt cgtgcgatga gtgataccgc agcgcatgat
     7321 gccaatatta cttttgatca atttattata gaagctggca agcgttcggc ccagatatta
     7381 atgacatttt tagaaaattc acctgtttaa ctcaccttat cgtgagtcaa ttagctggat
     7441 aatctctatt ttaggagtct ggtcacttct tgttttgaaa cctaaaaata agaattgcaa
     7501 tcacttgacc aaacgctata atgggttaag gaacgataga ttactaaaag gagtctaaaa
     7561 atgacactaa aaaaacacta ttatcttctc agtctgctag ctcttgtaac ggttggtgct
     7621 gcctttaaca caagccagag tgtcagtgca caagtttata gcaatggagg atatcgtcaa
     7681 ggtttgactg atgaaaaatc acacctgcaa tatagtaaag acaacgccaa acttcaattg
     7741 agaaatatcc ttaacggcta ccaaagtgac ctagggcgaa actactctag ctattattac
     7801 tacaatctaa gaaccgttat gggactatca agtgagcaag agattgaaaa atattatgaa
     7861 gagcttaaga acaagttaca tgatatgtac aatcattatt aagtcaaagg tttaaaaatg
     7921 gcgactagcc cctcaaaaaa agaggccaca ctcatttaaa tttaaatgag tgtggcctct
     7981 ttttgaggag acgcaagggg ttaaagggct gtgacataga gttgcttagc aatattttca
     8041 gggatgacaa gttccttgtc gccataacaa atcgtatagg tttttgcaaa ggtatcaatt
     8101 tgagttaagg ttagctcggt attgatactc aggtgatgtg tttctagata ctgaattaaa
     8161 tcaaagtggt cgtggattct gcttaggcga aaccgtccca actccgtgat ggtgttgaga
     8221 gttgtagtgt tcatttcgac aagaggctga ccatagcgag gaatagtccc tccgtgtggg
     8281 cagaaatctg gaaaatcaag gatcttgtct aaacggtcga taaaggtgtc tgagacagtg
     8341 tgctctaaga cttcagcttc ttggtgcact tcttgggtat tatagcccag ctgatgaatc
     8401 aaaaatacct caatcaagcg gtgtttgcgg tagaggttag caacaagagc atagcctttg
     8461 tcttttagca aatagccttt tgctttatct ttgacaatcc agccttgtga aatcattttt
     8521 ttaatcatct ctgaaacggc aggtgccgaa acgtgcatct tttctgcaac cattttattt
     8581 gtaatcttgg gttcttgttc tccaatttca tagatacatt tcaagtaatc ttctttatta
     8641 ggcgtcatat cgtttggcct ttctcttatt tataatgatt attatatcag aatttttagg
     8701 aaaaagcttg acaaaaagcc taaaagagga tatccttata gcataattaa ctaa
//
LOCUS       DAUCWO010000064         8523 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUCWO010000064 DAUCWO010000000
VERSION     DAUCWO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.064"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(113..553)
                     /locus_tag="VGY20_001441"
     CDS             complement(113..553)
                     /locus_tag="VGY20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4274284.1"
                     /translation="MTSSTQACVFSGVKIALMQDQHLVTILRDNKSTIPFPDTWDLPG
                     GGREGLETPFECVQREVMEELGIAISQDMVVWEKAYQGVMNPETYSIFMVAMISKDLV
                     KAIHFGEEGQAYKFVPVKDFLADKKAVPQLQERLAAYLAEKSDQ"
     gene            complement(581..898)
                     /locus_tag="VGY20_001442"
     CDS             complement(581..898)
                     /locus_tag="VGY20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEQ4274285.1"
                     /translation="MVLSPKPFEMIASGTKTIELRLNDEKRQKVQVDDAIVFVRTDDS
                     SQMLWTKVVELHYFDDFVQLYQSLPLDNCGYRKEELEMARPEDMERYYSQEQQAQYGG
                     SRH"
     gene            complement(912..1382)
                     /locus_tag="VGY20_001443"
     CDS             complement(912..1382)
                     /locus_tag="VGY20_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ4274286.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEMTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEGESYPYPRY"
     gene            1555..3528
                     /locus_tag="VGY20_001444"
                     /pseudo
     CDS             1555..3528
                     /locus_tag="VGY20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            3713..5470
                     /gene="pabB"
                     /locus_tag="VGY20_001445"
     CDS             3713..5470
                     /gene="pabB"
                     /locus_tag="VGY20_001445"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ4274287.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKTLDQVGPVIEKVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHHEMRQGNTYQVNYTIQLTQELNAADSFAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNQLTTRPMKGTTKRGVNTWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVEQYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPNPRGIYCGTVGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVEHQKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKNNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            5503..6069
                     /locus_tag="VGY20_001446"
     CDS             5503..6069
                     /locus_tag="VGY20_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ4274288.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKADA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQDPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            6102..7370
                     /locus_tag="VGY20_001447"
     CDS             6102..7370
                     /locus_tag="VGY20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ4274289.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLLD"
     gene            7459..7655
                     /gene="ssrS"
                     /locus_tag="VGY20_001448"
     ncRNA           7459..7655
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGY20_001448"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7656..7728
                     /locus_tag="VGY20_001449"
     tRNA            7656..7728
                     /locus_tag="VGY20_001449"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7689..7691,aa:Lys,seq:ctt)
     gene            7943..>8523
                     /locus_tag="VGY20_001450"
     CDS             7943..>8523
                     /locus_tag="VGY20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ4274290.1"
                     /translation="MKKTKLIFSLTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKDGDKIAVFSVPFDWNYL
                     SEGKVTAYTYGGVTPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLIAQHQLYSSGSSYKSGKLVFHTNDNSDK"
BASE COUNT         2561 a         2027 c         1607 g         2328 t
ORIGIN      
        1 caatttctct agtagtcata tacctccatc taatctctca atcgtccttc caaactccca
       61 tagacttggc catttctcac aatccttaaa cactgaaata ccaacactca tctcactggt
      121 cacttttctc agctagataa gctgccaatc gttcttgtaa ttgaggcaca gccttcttat
      181 ccgctaaaaa atccttcacc ggtacaaact tgtaagcctg accttcttct ccaaaatgaa
      241 tggctttaac aagatcctta ctaatcatgg ccaccatgaa aatagagtaa gtctccggat
      301 tcataacccc ttgataagct ttttcccaga cgaccatatc ttgcgaaatc gcaattccta
      361 gttcttccat cacttcacgt tgcacacatt caaagggtgt ttctaaacct tctcggcctc
      421 caccaggcaa atcccaggtg tcaggaaaag gaatggtaga cttattatct cttaaaatgg
      481 tcacaaggtg ttggtcttgc attaaagcaa ttttcacccc agaaaaaacg catgcctgtg
      541 tcgatgatgt cataatcgtc tcctctacag cactgataac tcaatgccga ctacccccat
      601 actgggcttg ctgttcctga ctataatagc gttccatgtc ttctggacga gccatctcca
      661 gttcttcttt ccgatagcca cagttatcta agggtaacga ctggtaaagc tgaacaaagt
      721 cgtcaaaata atgaagctca accacctttg tccacaacat ctgactacta tcatctgttc
      781 taacaaatac gatggcatca tcaacttgga ccttttgccg cttctcgtca ttcagacgca
      841 attcaatcgt ctttgtacct gaggcaatca tctcaaaagg tttaggactt agcaccatct
      901 cgtgtgtcac cttagtacct tgggtaaggg taactttctc cctcttctaa tccttgttga
      961 ccttcttcaa aagcttgcgc gttccactcc aagaagaaat cagccttgct gtcatccgct
     1021 aagaaatcca ttaaatcgct gtgcccttca atagcaacat cgttgagaaa ttgcgcaaag
     1081 taagctaaga attcacgaga aaagcccttt ttagcgtcaa aaggaatagt aaccaaataa
     1141 tctgctgcat caaattgaga cttggcaggg ttgtaaaaga gcacatagtc ctcaaagaca
     1201 atatcatctt gggtcatttc gccactatca tcaatggttt ttatggcctc tttattttga
     1261 gcttctaaaa caaaagtcac ttcaaccgca tgatttttct tgtcccaatc catagcataa
     1321 tcatactgaa aatgcttatc catttcttct tctaatactg ataaaaagcc atattttgcc
     1381 atctaattgt cctttttcct aacgattatt tgcttccatt gtagcataat ttgaactaac
     1441 tttcactggc aaatgctgac tgatgggcct tctgtatttt agtaagcgtt ttacccctta
     1501 ctttcctccc attatgttaa actaataagc gattatgatg ttaggagaaa aagaatgaga
     1561 tattctagac gctacccttc tcagacaaga cagatgcttc ttgccttgtt attaatgact
     1621 gccagcctac aagctggagc tatgactact tatgcggatg aggttaatac caaccatcaa
     1681 ccactaacgg cccaagtgga agctagtaag ccgacagcca tggaatctgt cacttcacct
     1741 gcagaccaga cccatccaat ctcaacgcaa gaggcttcgt ctcctttaca ccctttaaca
     1801 acagaggcca ctcctgccgc ccaagaatct ccgattacct tagaagacta taaggtagcc
     1861 agtgcgagcc aattagctga gtgggctcgc cagcaacggg tcacaggcca acagctgcta
     1921 gattttgcct tggaaaccat caaagaaact aaccctgagc tcaataatgt catttctctg
     1981 cgtgaaccat tggctcgaca agagagcgag caaatgacag atgaggggca acccttttac
     2041 aaggttccta ttctggttaa agggttagga cacaccgttg ctggaagtag caacaccaac
     2101 gggcttgcct ttttgaaaga caaaaccagc tcatctacca gcgcctttgt caaacaactc
     2161 caaaaggctg gttttatcgt ggtagggcag tcctctttcc ctgaaatggg atggattaat
     2221 gtgacgaatt ccaatcttta cggaaataca cataacccat ggcagcttga ccaaaatcct
     2281 ggcgggtcct ctggtggctc tgcagctgct gtggcttcag gacaagtcag tctagccagt
     2341 gcttctgatg gtgggggctc aacccgcatt ccagcttctt ggagtggctt gattggccta
     2401 cacccaacac gtggcatttt agagggaaat ccgacttctg aacgtagtaa tgttagtcat
     2461 tttgctctaa ctaagagcat ggaagacact gaaaagctct tccaattcct cttaaaagac
     2521 aaggctaaag cgcaacaaaa cccacagcgt ttagacacaa gtatccccat tgcttacagc
     2581 acccagacac cagcttgaac accaattagt gaggaagcta ttgctgcgat caaagaagct
     2641 gtagcctttt tacagaagca aggttatcaa accgtagaag tgccttaccc cgttgatggt
     2701 aagctcatga tgcaatacta ttacactatt gctgcttctg cagcgccatc tatcaatttt
     2761 atggcacaac aaaccttgaa acgccccctt caaaaagagg atgtcgaact cctatcatgg
     2821 gcgctctacc aaactgggaa agacttgacc aaagaggatg tcaacaaagc ctgggagggt
     2881 atcgcagcca tgaccgagca attaaaccag ttctaccaaa aataccccat cttcttaaca
     2941 ccgacaacag cctatcccgc ccctgctgct gattatcacc acattcctaa agatctagta
     3001 gcacagctca gtgacatgtc aggcttgagc aaagaagaaa aattggacct tatttaccgg
     3061 caatggttac ctgcatggac gctaacacca tttacccagc ttgctaacct gacgggaaca
     3121 ccatccttgt ctttaccaac ccatctgacg aaaagcggtc tacctcttgg cattttggtg
     3181 aatagcggag ctcataacga tagtctcctg ttacaactgg gacagctctt tgaaaaagca
     3241 aatcgtttcc atatcctgac ggctggcaaa aaaggattgc cagaaactcc tgcgcatgag
     3301 cataacctct caactcaatc tgaaaacaaa caaggcgttg ccattccagt gacttatcaa
     3361 accaagggat ttactactgg accaaccaag ggccagaacg gcttagtaac cctccctcaa
     3421 actggtgatg gtcagtcaaa aggtgtgttg ttaactagct acatcagcct tttccttgga
     3481 accctcttct tagcaggaag cctctggagc aataaggtaa aagactaggc tattgtcgtc
     3541 ggagatttaa cctacctaaa cattcactgc tgcttgcctt acgataagag tatattgtag
     3601 tcaatcaaga ctgaactttc ttagtgaaag ttcagtcttt ttacgtggcc aaaagacagt
     3661 tgttgcctgt tctcattagg cttagcaacc gcttttatga tataatttgg gtatgcatag
     3721 aaaaacgatt attgatttca aagagctcgg gcaacgctat ctttttgatg agccattagt
     3781 agaattggta gccaaaacct tagatcaggt cggcccagtc attgaaaaag tacaacacta
     3841 ccaacagctg ggctattacg tggtgggcta tctcagttat gaagcagctg ctttttttga
     3901 caatgcctta caaacccata atgacagact aggcaacgag taccttgcct attttacagt
     3961 gcacaaaacc tgtcaaaaaa aagacctacc tcttgattat gacagcatta ctatccctaa
     4021 ccaatgggtc agcgccactc aaaaagaagc ctatcaaaaa gccattgaaa ccattcacca
     4081 tgagatgcga cagggcaata cttatcaggt taactatacc attcaattaa ctcaggaact
     4141 caatgcagct gacagcttcg ccatttacaa taaactcgtc gttgaacagg ctgctggcta
     4201 caatgcctat atcgcccatg atgagtttgc cgttatttca gctagtcctg agttgttctt
     4261 taaacaggaa gggaatcaac tcaccaccag acctatgaag ggcaccacca agcgcggggt
     4321 taacacctgg ctggatcaac aagaacatga ttggctccaa gctgacggga aaaatcgctc
     4381 tgaaaacatg atgattgttg acttgctccg caatgacatg ggaaaaattt gccaaacagg
     4441 cagtgtttgt gtcgataggc tctgtgaggt ggaacaatac tctaccgtct ggcaaatgac
     4501 ctcaaccatt gttggtgacc tcaaagcgga ctgtgacctg atagacattc tcacagctct
     4561 ctttccttgt ggctctatca caggggctcc taaagtctcc accatggcga ttatcacatc
     4621 tctggagcca aacccaaggg ggatttattg tggaactgtt ggcatttgtc tacctgacgg
     4681 tcgccgtttc ttcaatgttc ccattagaac catccagctc agtcataatc aagctactta
     4741 tggtgtcgga ggcggtatca cttggcaaag caagtgggaa gacgaatacg aagaagtcca
     4801 tcaaaaaaca gcttttctct atcggcacaa acaaatcttt gacctcaaaa caaccgccaa
     4861 ggtggaacac caaaaaatag ccttccttga gcaacacctc aatcgcctta aagaagcagc
     4921 cacttatttt gcttaccctt acgatgagaa agctttgcag aaacaactgt caacctattt
     4981 ggaaaataag aataatgctg cctaccgttt gatgattcgt ttatccaaag atggaaagat
     5041 tagcctctct gatcaaccct tggaaccttt gtcagccgat tttctgacag ctcaactctc
     5101 tttacagaaa aaggacgtga cagcttcgcc ttttacctac tttaagacca gctaccggcc
     5161 tcatattgag caaaaatctt atgaacagct attctataac caagcggggc aattattaga
     5221 aacgtccatt ggcaatctct ttgttcagct gggtcagacc ctctacacac caccagtggc
     5281 tgtggggatt ttaccaggac tttttcgcca agagctgcta gctactggtc aagctcagga
     5341 aaaagaggtg acactagctg atttaaaaga ggctagcgct atttttggag gaaatgctgt
     5401 ccgtggcctc tatcctctca accttgagct tactcacctt gatgccctct tagctaagag
     5461 tcaagcctaa actgccactc atcttttgtt attaaggaac ctatgatact cttaattgat
     5521 aattacgatt catttaccta caacctcgcc caatatttaa gtgaatttga cgagacgatt
     5581 gtcttgtata accaagaccc aaacttatat gacatggcca aaaaggccga cgcgctagtc
     5641 ctctcacctg gtcctggttg gcccaaggaa gccaaccaaa tgccaaaact cattcaagac
     5701 ttttaccaaa caaaacctat cttaggagtg tgtctgggac accaagcgat cgctgaaaca
     5761 ttagggggaa ccttacgctt ggccaaacgc gtcatgcacg ggagacaaag caccattgaa
     5821 acgcaagacc ctgctagtct ttttcgctcc ctgccacaag agatcaccgt catgcgctac
     5881 cattccatcg ttgtggatca gttaccaaaa ggttttagcg taactgctag agactgtgac
     5941 gatcaagaaa tcatggcatt tgaacaccac accctgccac tttttgggct acaatttcac
     6001 ccagaaagca tcggaactcc tgatggcatg accatgattg ctaacttcat cgcagctatt
     6061 ccccgttaat cccatccccc aaaagaaagg aggacagtta catgcctgat catttagcgc
     6121 tcagaatgcg ccccaaaacc atttcagagg tcatcggaca aaaacacctt gttggcgaag
     6181 gcaaaatcat ccgtcgcatg gtggaagcaa atagactatc gtccatgatc ctctatggcc
     6241 ctccaggaat cggtaaaact tccattgctt ctgctattgc aggtactacc aagtacgcct
     6301 ttcggacctt taatgccacc gttgatagca aaaaacgtct ccaagaaatt gcggaagaag
     6361 ctaaattttc tggtggcttg gtattattac tagacgagat tcatcgcctt gacaagacaa
     6421 aacaagattt tcttttacct ttacttgaga atggcaccat tatcatgatt ggcgcaacca
     6481 ctgagaaccc cttcttttca gtgacacccg ctatcagaag cagggtccag atttttgaat
     6541 tggaaccact ttctaacgag gatattaaaa cagccattca gctagcaatt tccgacaaag
     6601 aacggggatt tcctttttta gtgacaattg atgatgaggc gctagatttc attgttaccg
     6661 ccacaaatgg agacctacga tctgcttata attccttgga tctagcagtc atgtctactt
     6721 ctcctaatga agatagtagc cgtcatatta gtctagagac aatggaaaat agtctgcagc
     6781 gtagttatat taccatggat aaaaacgggg acgggcatta tgatgtttta tctgccttac
     6841 aaaaatccat tagaggttct gatgtcaatg ccagcctgca ctacgccgct cgtttagtag
     6901 aagctggaga cttgcctagt ttagcaagac gattgaccat tattgcttac gaagatattg
     6961 gcttagccaa ccctgacgcc caagttcata ccgtcaccgc actagatgcc gctcaacgca
     7021 ttggttttcc agaagctcgt attcttatcg ctaacgtggt cattgatttg gctctatcac
     7081 ctaaatccaa ttcagcttat ttagctatgg atgcagcttt agctgatttg agaacatcag
     7141 gtaacctccc cattccaagg catcttagag acgggcatta cgctggcagc aaggacctag
     7201 gcaatgccaa agattacctc tacccacacg cctaccctga aaaatgggtc aaacaacaat
     7261 acctaccaga taaattagta ggacatcatt attttgaagc caatgaaacc ggtaaatacg
     7321 aacgcgcact tggttcaaat aaagagcgta ttgataagct cttagactga ctgctagttt
     7381 taaaaatcgc aaccgatttc aaaaatcgat aattttgctt gaaaaaattt taaaaagtgg
     7441 tattataaaa gtacagaaac attgctgtgg catacgaatc cacatctaag tgttgaccga
     7501 ctatttcttt gtattttcgg gaaacaaaga ctctttctag tctgcaagcc gtttcacgcg
     7561 gacgcaactt ccaagaaaga gcgcgttacc cacctgctta tactgcgcgg gctcaataca
     7621 aaaacgtgga tcaccggtgc caatacagca ttcttgcctc cttagctcag ttggtagagc
     7681 agtagactct taatctatgg gtcacaggtt cgagccctgt agggggtata caactataca
     7741 taaaaaagcc tttgtttaaa ggcttttttg attgtttcat aactaatcgg cttaattaaa
     7801 agcgtcaatc cacttttagt atcatgtctc aattttcatt tgatagtcag caagtatatc
     7861 tacaaaattg ttaatgatta tttcttttga aagtgttata ttaaaacata ataaaataat
     7921 aactcctgaa aagaggctat ttatgaaaaa aacaaaactt attttttctt tgacttcaat
     7981 attcattgca ataatttctc gtcctgtgtt tggattagaa gtagataata attcccttct
     8041 aaggaatatc tatagtacga ttgtatatga atattcagat acagtaattg attttaaaac
     8101 cagtcataac ttagtgacta agaaacttga tgttagagat gctagagatt tttttattaa
     8161 ctccgaaatg gacgaatatg cagccaatga ttttaaagat ggagataaaa tagctgtgtt
     8221 ctccgtccca tttgattgga actacttgtc agaaggaaaa gtcacagcat atacgtacgg
     8281 cggagtaaca ccctaccaaa aaacttcaat acctaaaaat atccctgtta atttatggat
     8341 taatggaaag cagatctctg ttccttacaa cgaaatatca actaacaaaa caacagttac
     8401 agctcaagaa attgatctaa aggttagaaa atttttaata gcacaacatc aattatattc
     8461 ttctggttct agctacaaaa gtggtaaatt agtttttcat acaaatgata attcagataa
     8521 ata
//
LOCUS       DAUCWO010000065         8455 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUCWO010000065 DAUCWO010000000
VERSION     DAUCWO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.065"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            18..98
                     /locus_tag="VGY20_001451"
     tRNA            18..98
                     /locus_tag="VGY20_001451"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VGY20_001452"
     tRNA            105..175
                     /locus_tag="VGY20_001452"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VGY20_001453"
     tRNA            187..259
                     /locus_tag="VGY20_001453"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="VGY20_001454"
     tRNA            268..339
                     /locus_tag="VGY20_001454"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="VGY20_001455"
     tRNA            359..442
                     /locus_tag="VGY20_001455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            1525..1833
                     /locus_tag="VGY20_001456"
     CDS             1525..1833
                     /locus_tag="VGY20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ4274291.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSAVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1888..2100
                     /locus_tag="VGY20_001457"
     CDS             1888..2100
                     /locus_tag="VGY20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ4274292.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2210..2653
                     /locus_tag="VGY20_001458"
     CDS             2210..2653
                     /locus_tag="VGY20_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ4274293.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKVLNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2657..3376
                     /locus_tag="VGY20_001459"
     CDS             2657..3376
                     /locus_tag="VGY20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274294.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEAETDDEKGGHGHA"
     gene            3369..4184
                     /locus_tag="VGY20_001460"
     CDS             3369..4184
                     /locus_tag="VGY20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ4274295.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVIGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4224..4607)
                     /locus_tag="VGY20_001461"
     CDS             complement(4224..4607)
                     /locus_tag="VGY20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ4274296.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMEEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4658..5914)
                     /locus_tag="VGY20_001462"
     CDS             complement(4658..5914)
                     /locus_tag="VGY20_001462"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ4274297.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LNNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6006..8318
                     /locus_tag="VGY20_001463"
     CDS             6006..8318
                     /locus_tag="VGY20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4274298.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
BASE COUNT         2451 a         1455 c         1884 g         2665 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgtgggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tgtaaacctt gttttttgtt
      481 ttgatcttag gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttt aaaaaggttt gtcactacta ggtgcttttt taactataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggtcgggt atcgggtaat ggagtgtggt tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggctaga gccttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggcacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agttcaagac tttgtagagt tggagaattt ggttctttat
     1081 gaggaagaag tgcaggtaat tcgttgagcc agtcgtgaag atattttagg tatgattgat
     1141 gaaggttcgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggt
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgcaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1381 gatttatcga aggttatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1561 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1681 gatattggta taccgattgg tagtgctgta ccttattgct tgctttcagg ttgtgcccaa
     1741 gttactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1801 ttggttaaac cagattttgg tatctcaact tgaacaattt ttctagatat taattgtaaa
     1861 accatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     1921 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     1981 tttattcaaa aaataaaaga gaaaacactg caagctggtg ctgctactac tttgatgaca
     2041 ggaagtggac cgactgtttt tgctttgtgc cagaccgaaa aacaaactat aatagtttaa
     2101 aaggactttg caaggaagtt tttaaggtga gaatgttgta atattttgtt gggattgctt
     2161 ctttggaagc ttcatgttat aattaactgg ttaatgaaag aggagattta tggggacttt
     2221 agaaaaaaaa cttgataact tagtaaatac tattttatta aaagcagaaa atcagcatga
     2281 gttattattt ggagcttgtc aaagtgacgt taagcttact aatacgcaag aacatatttt
     2341 aatgttacta tctcagcaac gtctcactaa tacagatttg gctaaggtat taaatattag
     2401 tcaggcggca gtaactaagg ctatcaagag tttggtcaaa caagacatgc tagcaggaac
     2461 taaggatacg gttgatgcta gggtgactta ttttgaatta accgagttag ctaagcctat
     2521 tgcatcagaa catacccatc atcatgatga aactttaaat gtttacaacc gtttattaca
     2581 aaaattctct gcgaaagaat tagagattgt agataagttt gtaacagttt ttgctgagga
     2641 attagaaggg taatccatga gatacatatc agtgaaaaat ctctcctttc aatatgaaag
     2701 tgagccagtt ttagaaggga tcacttatca tttagatagt ggagaatttg tcaccatgac
     2761 cggtgaaaat ggcgctgcaa agtcaacctt aataaaagca accttaggaa ttttacaacc
     2821 aaaggctgga cgagttacta ttgctaaaaa aaataaagac ggtaaacaat taaggattgc
     2881 ttacttgccg cagcaagtag ctagctttaa cgctggtttt ccatccaccg tttacgagtt
     2941 tgtcaaatca ggtcgctacc cacgtagtgg ttggtttaga catttgaaca aacacgatga
     3001 agagcatgta caagcaagct tagaagcagt cggcatgtgg gaaaaccgtc ataagagaat
     3061 tggtagttta tcaggtggtc aaaaacaacg tgtggttatt gcccgtatgt ttgcttctga
     3121 ccctgatatt tttgtgctag acgagccaac aacgggaatg gatagcggta ctactgatac
     3181 cttttatgaa ctgatgcacc acagtgcaca tcaacatggg aaatccgttc tgatgattac
     3241 ccatgaccca gaagaagtga aggcttatgc tgatcggaac attcatttag tcagaaacca
     3301 aaaacttcct tggcgttgtt tcaacattca tgaagctgaa acagatgacg aaaaaggagg
     3361 tcatggtcat gcttgatatt ttatcctatg atttcatgca acgggcggta atggcggtag
     3421 ttgccattag tatttttgct ccgattttag gtattttcct tattttacgt cgtcaaagtt
     3481 tgatgagcga tacccttagt catgtttctt tggctggggt agcgcttggg gtagtcattg
     3541 gtatttcacc aaccatcact actattattg ttgtggtttt agctgctatt ttgttagaat
     3601 acctgcgtgt agtttacaaa cactacatgg agatttcaac ggcgattttg atgtcacttg
     3661 gcttggccct atctctgatt attatgagta agtcgcatag ttcatcaagc atgagtttag
     3721 aacaatacct ttttggatcg atcatcacga ttagtatgga acaagttgtc gccttgtttg
     3781 ctattgctgc gattatttta atcttgaccg ttctcttcat tagaccgatg tacattctga
     3841 cctttgatga agatactgct tttgtagatg gtctgcccgt tcgcttgatg tctgttctat
     3901 tcaatatcgt cactggggtt gctattgctt tgaccattcc agcagcagga gcacttttgg
     3961 tttctaccat tatggtcttg ccagcaagta tcgcaatgag attgggtaaa aactttaaaa
     4021 cagttatctt actgggaatt gtcatcggtt ttagcggtat gttatctggt attttcttat
     4081 cttatttctt tgaaacgcca gctagtgcca ctattaccat gattttcatt agtattttcc
     4141 tcttagttag tctaggtgga atgcttaaaa aacggttatt ttaataagaa aaatcctcac
     4201 atgtttaaca tgtgaggatt tatttataaa tgctttaaaa agggggtaag gtcccaattt
     4261 tcttgattaa tggtgatgct ctcccaaaag ctatcattag ttaaacgagg aatcaaatga
     4321 aggtgaaaat gagtcccctc ctccatcaac tccttgtcat tgctgacgct agtgacgcct
     4381 gaaatgggaa ggtgctgctc tagcttagca actagagcgg cttgtaagtc gatgagatca
     4441 tagcgcactt cctttggtag ttgtgttaga ctagtgtaat ggtctttgga aatcagtaaa
     4501 aggtgcccct tttggatagg atcaatatca aaaacaacct tgaaatattg actttgagtc
     4561 agtagctgat ttgctttgag ctgatggcaa aagatacatt ctgtcatagg tacatctcct
     4621 tatgaaaaaa cgagccagag ctcgtttaat cggttatcta gtaagtgaga acagcgtatt
     4681 ttttcttacc gcggcgaata acggttaatt gatcatcaat tttatcatca ttatttaatt
     4741 ggtaatctaa atcttgaacg cggtctccgt taatgtagat tgctccattt tgcacgtctt
     4801 cacgcgcctg gcgttttgat ggggaaatct tagcagctac taaaatctcg acaatattat
     4861 gattgtctat tgattgaacg tgataatttg gtacattgct caatccttgt tttaattcat
     4921 tagcagaaag atttttgatg tttccagcaa ataactgctc ggtaatgtta agagcttgtt
     4981 tataagcttc ttcgccatga accaaggtaa ccacttcgcg tgccaaggtt ttttgagcga
     5041 gacgctcgtg acgagcggca ttaaattgag tttcaatttc tgcaatctca tctagagata
     5101 agaaggtgaa gattttcaag aaacgcactg catcatcatc catgacattt aaccagaatt
     5161 ggtacatctc gtaaggagac gtcttatcgg catcaagcca gacggcatta ccttctgatt
     5221 taccgaattt tttcccggtt gagtcagtga tgagtggtac agtcatgacg tgcccagttt
     5281 tatcagcctt tttgcggagc aattcagtac cagctgtcat attgccccac tggtcagagc
     5341 cgccaatttg taaggttaca ttatgcttgt cattgagttc gtagaagtcg tagccctgca
     5401 tgatttggta agcaaactca gtgtaagaaa tgcctgtttc aatgcgtttt ttaacagagt
     5461 ctttactcat catgtagtta acggtgaagt atttaccgac atcacggagg aagtcaataa
     5521 agctgatttg cgagaaccag tcgtagttgt tgacaagctc tgctttatta tcaccatttt
     5581 caaaatcaag gaaagtagac aactgccctt taatcttgtc actccactcc aaaactgttt
     5641 ctttggtttg aaggctgcgc tctgcatctt taaaggaagg atcaccaatt aaacctgtgg
     5701 caccacccac aagagcgtaa ggtttgtgcc ctgctagttg caagcgacga gatgttaaga
     5761 tagccactag atggcccaga tgaaggctgt cagcggttgg atcataaccg gtataatagg
     5821 atacttgccc ttctgttaat gctttgacaa gggcttgttc atcagttgtt tgaaagacca
     5881 agccacgagc tttgagttct tcaaaaatat tcataggctt tctccatttt cattatagtt
     5941 tcgaatcatt atatcaaaaa acgctactgc tatgcaagct ttatcaaagt ttggtataat
     6001 gataaatgag gtattttatg gtgaaatgga acacgaaaca aaagcgtata agtcatcaaa
     6061 gattaggtct cttggatttg gggccggtgc tattacgtac gttgagacta ctgtctaact
     6121 ttttttatat tgttatcttc ctttttggaa tgatgggatt tggtatggca tttgggtatt
     6181 tggctagtca gattgaatct gttaaggtac caagtaaaga aagtttagtc aaacaagttg
     6241 aatcattaac gatgatttcg caaatgaact attctgataa tagtttaatt tctactttag
     6301 atacggattt acttcgaaca ccagtagcta atgatgcgat ttcagagaat atcaaaaaag
     6361 ctattgtatc aacagaagac gaacattttc aagaacataa aggtgtcgtg ccaaaagctg
     6421 tttttcgggc aacattggct tctgtattgg gatttggaga agctagtgga ggttcgacct
     6481 taacacagca attagtcaag caacaagttt tgggagacga tcccacattt aagcgcaagt
     6541 ctaaggagat cgtttacgct cttgccttag agcgttatat gtccaaagac aatatcttat
     6601 gtgattatct taatgtttca ccttttggcc gtaacaacaa gggtcaaaat attgctggtg
     6661 ttgaagaagc tgcgcgtggc atttttggcg tttctgccaa agatttaacg gtgccacagg
     6721 cagcattttt ggcgggtctt ccgcagagtc ctattgttta ctctccttat ttgtcaacgg
     6781 gacaactgaa gtcagaaaag gacatggctt atggcatcaa gcgtcagcaa aatgttctct
     6841 ataacatgta ccgtacaggt gttctgtcta aaaaagaata cgaggactat aaggcttatc
     6901 cgattcaaaa ggattttatt caaccgggaa gtgcaatagt aaataatcac gattaccttt
     6961 attacacggt gttagcggat gctaagaaag ccatgtatag ctatttgatt aagcgagata
     7021 aggtgtctag tcgtgacttg aaaaatgacg agactaaggc tgcttatgaa gagagagcct
     7081 taacagaatt gcaacagggt ggctatacca tcaccacaac cattaataag cctatttaca
     7141 atgcgatgca gacagcggca gctcagtttg gtggtttgtt agatgatggc actggtacag
     7201 ttcaaatggg aaatgtcttg acagacaatg cgactggtgc tgtgttaggt tttgttggtg
     7261 gtagagatta tgctctgaat caaaataatc atgctttcaa tacagttaga tcgccaggtt
     7321 ctagcattaa accgataatc gcttatggtc ctgctattga tcaaggttta atggggagtg
     7381 ctagcgtttt gtctaattac ccaacaactt actcgagtgg tcaaaaaatc atgcatgctg
     7441 atagtgaagg aacagccatg atgccacttc aagaggccct aaatacttct tggaacatcc
     7501 cagctttttg gacacagaaa ttactgcgtg aaaaaggggt cgatgtcgaa aattatatga
     7561 caaaaatggg ttataagatt gcagactact cgattgaaag tttacctcta gggggcggta
     7621 ttgaagtctc ggttgctcaa caaaccaatg cttaccaaat gctttcaaac aatggcttat
     7681 atcaaaagca atatattgta gataagatta ctgctagcga tggtacagtc gtttacaaac
     7741 atgaaaataa gccaattcgt attttttctg cagcaacagc tacgatttta caagaattgt
     7801 tgagaggtcc gattacttca ggcgctacga ctactttcaa gaaccgtttg gcggctatta
     7861 atccgtggct tgctaatgct gattggattg gtaagaccgg aacaactgag aattatacgg
     7921 atgtttggct agtcctgtct actccaaaag ttaccttagg cggttgggca ggacatgatg
     7981 acaatacctc attagcgcca ttaacaggat ataacaataa ttctaattac cttgcctatt
     8041 tagctaatgc cattaatcag gccgatccca atgttattgg agtagggcaa cgcttcaact
     8101 tagatccagg agtcattaag gcgaatgtct tgaagtcaac aggtttacaa ccaggaactg
     8161 ttaatgtcaa tggacatact ttttctgttg gtggagaaat gaccaccagt ctatggtccc
     8221 aaaaaggacc gggggctatg acttaccgat ttgctattgg tggcacggat gccgattatc
     8281 aaaaagcctg ggggaacttt gggttcagaa aaaattaggt tgccatttat cgataaaaag
     8341 ggtataatag caataactat ttgtcgtcca atctagctga aatattgtcc agttaggaag
     8401 aacagcagtt aaatcacact gataaagtca gatttagctg ctctttttgt gtcta
//
LOCUS       DAUCWO010000066         8412 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUCWO010000066 DAUCWO010000000
VERSION     DAUCWO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.066"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            56..1387
                     /locus_tag="VGY20_001464"
     CDS             56..1387
                     /locus_tag="VGY20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ4274299.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLTVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHTSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            1419..2585
                     /locus_tag="VGY20_001465"
     CDS             1419..2585
                     /locus_tag="VGY20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ4274300.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(2666..3717)
                     /locus_tag="VGY20_001466"
                     /pseudo
     CDS             complement(2666..3717)
                     /locus_tag="VGY20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            3953..4684
                     /gene="aphA"
                     /locus_tag="VGY20_001467"
     CDS             3953..4684
                     /gene="aphA"
                     /locus_tag="VGY20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ4274301.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            4862..6394
                     /locus_tag="VGY20_001468"
     CDS             4862..6394
                     /locus_tag="VGY20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ4274302.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPILA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            6557..7171
                     /locus_tag="VGY20_001469"
     CDS             6557..7171
                     /locus_tag="VGY20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ4274303.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQSYI"
     gene            7330..>8412
                     /locus_tag="VGY20_001470"
     CDS             7330..>8412
                     /locus_tag="VGY20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ4274304.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAAT"
BASE COUNT         2484 a         1416 c         1691 g         2821 t
ORIGIN      
        1 atgcttttga catgttctct tttatactag ccttatcact aaaggaggtt gtttcatgga
       61 aacaataagc aagaaaatgc ctcaaaaaga cttgtctgag cattcaaaag catggcaaaa
      121 taggcgaata ggctccgttc ccttgactgt ttatttggtt ttagctacct taattttagt
      181 cacaggctgg ctccaacagc tgcctgttaa tatgctgggt ggctttgctg ttattttaac
      241 ccttggttgg ctactaggaa ccattggagc gaccattcca ggcctcaaac attttggtgg
      301 cccagctatt ttatcactct tagtcccttc gattttggtt ttttttaatc tcctcaaccc
      361 caatgtctta gaagcgacga atgtcttgat gaagcaagcc aattttcttt atttttatat
      421 tgcctgcctg gtctgcggta gtattttagg catgaatcga aaaatcttga ttcaaggtct
      481 ttttcggatg attattccca tgttacttgg aatggtttgt gcaatgggtg tagggacttt
      541 ggttggcgtc attttaggat tagactggca acataccctc ttttatgttg tgacgccagt
      601 ccttgctgga ggaattggtg aaggtatttt acccttatct cttggttaca gtgccattac
      661 aggagtcgga agtgagcaat tagtggctca actgattcca gccaccatta ttgggaattt
      721 ctttgctatc ttgtgcacgg ctttgctcaa tcgttttgga gagaagcaca caagttattc
      781 tggtcaaggg caactggtta aaattggtca cagcgaagac atgtctgatg ccctaaaaga
      841 caattctgga gccctagatg ttaaattaat gggggcaggc gttttaacag cttgttcact
      901 ttttattgct ggtggcttgt tacagcattt aacaggtttt ccaggaccgg tgttgatgat
      961 tattctggca gccttcttga aatacctgaa tgttattccc caagaaaccc aaaatggtgc
     1021 caaacaactt tataaattta tttccagcaa ctttactttt ccgttaatgg cagggcttgg
     1081 tctgctttat atccccttaa aagaggttgt ggcaaccctt agctggcaat attttattgt
     1141 ggtgattagt gtggtgttaa cggttgtcag tgtcggtttt tttgtctctc gtttccttaa
     1201 catgaaccca gtggaagcag ctattatttc agcttgtcaa agtggtatgg gtggaacagg
     1261 ggacgtggct attttgagta cggctgaccg catgaatcta atgccgtttg cccaagtcgc
     1321 tactcgtcta ggcggggcca ttaccgttat caccatgaca gctattttaa gaattatttt
     1381 tagttaatcc aaaccaataa aaaagagagg tatttcctat gaaaaatcaa ttaggtcaac
     1441 ttgcccttga acaagctaaa acgttcggcg gtaaattgga agttcaacca aaagttgaca
     1501 ttaaaactaa acatgatttg agcattgcct atacaccagg tgtagctggt gtgtcatctg
     1561 ccatcgcaaa agataaaaca ttagcatatg acttaaccac caagaaaaat acagtagccg
     1621 ttatcagtga tggtactgcg gttcttggtc tgggggatat tggccctgaa gcagccatgc
     1681 cagttatgga aggaaaagca gcccttttta aagcatttgc aggtgtcgat gctattccga
     1741 ttgtattaga taccaaagat actgaagaaa ttattagtat tgtcaaggcg cttgcaccaa
     1801 cctttggtgg tattaattta gaggatatta gtgcgccccg ctgttttgaa attgagcaac
     1861 gcttaattaa ggaatgtcat attcctgttt tccatgacga tcagcatgga acagctattg
     1921 tggtgttagc agccatcttt aatagtttga aacttcttca aaaatccctt gatgaggtat
     1981 ccatcgtggt gaacggtggt ggatctgcgg gattatccat tacccgtaaa ttattagcag
     2041 caggtgctac gaaagtgaca gtagtggata aatttggcat tatcaatgaa caagaagccg
     2101 ctcaattagc gccacatcat ttggatatag cgaaagtcac taatcgtgag tttaaatcag
     2161 gtaccttaga agatgctctt gaaggagcag atatcttcat tggcgtatca gcaccaggag
     2221 tcttaaaagc agagtggatt agtaaaatgg cagcgcgccc agtaattttt gccatggcca
     2281 accctattcc agagatttac cctgatgaag cccttgaagc gggggcttac attgtaggta
     2341 ctggccgcag tgattttcct aaccagatta ataatgtcct tgcctttcca ggtattttta
     2401 ggggagcatt agatgcacgg gcaaaaacca ttactgttga gatgcaaatt gctgccgcaa
     2461 aaggtattgc cagtcttgtc ccagatgacg ctttgagcac tactaatatt attccagatg
     2521 cctttaaaga aggagtcgct gagattgtcg ctaaaagcgt caggtcggta gtccttaaat
     2581 catagaatat atgaaaaaag agaaagttat actttaaatc acaaaataag tagaagcgta
     2641 tttctagctt ctacttattt tgtgattagc taatatcatt atcaataata actttcaagg
     2701 cgttatttgc accagcatgt ttaaaggttt cataagcttt ttcaacttct gaaagtttaa
     2761 aataatgtgt aataagtctt gttgcgtcaa tctttccagt tttcaagaca tttagtagca
     2821 tttcagttgt attggcatta acaagtccag tgtttaaggt gatattttta atccataatt
     2881 catccaggtt gaaatcaact ggtttcccat gaaccccaac gttagcaata tgcccaccta
     2941 ctgatataat tttttggcaa atatcaaatg tcgcgggata accaacacac tccatggaaa
     3001 tatcgacacc acgtccattg gttatatcgt caatgatagc cttgacttct tcactacttc
     3061 ctgaacaaat cgtgtgtgtg gctccaaacg ttttagctgc ttcaaggcga ttttgagata
     3121 agtcgaccat aataatattg gctggagaga aaagttgaac ggttagtagg gctgcgagtc
     3181 ctactggacc ggcaccaata atacaaacat tatctccagg cttgacatgt gatggcaaga
     3241 caccaatttc atatgaagtt ggtaaaatat cagaaagcat gaccagtgcc tcatcatcaa
     3301 tcgtatcagg agcatgatag agactaccat ctgcatgtgg gatatggaca tattctgctt
     3361 gtgttccatt aattaaatgg ccaagaatcc aaccgccatc ttcacaatgt gacgaaaggc
     3421 ctcgtttaca ataataacag gtatgacatg atgtgacgca agaaataatc actttatctc
     3481 cgattttaaa gttagtgacc gcatcaccga tctccttaac aattccaatt ccttcatgac
     3541 caaggattgt tccttcttta catgcgggaa catccccacc taagatatgc aaatcagttc
     3601 cacaaataga gtggttttta ccaactggac gatagcatct gtaggcttta taatgactgg
     3661 ttttggtttg tctatgagct gtagatttcc ggttgaaagg taagttgctg ctttcatatt
     3721 taactcttta taaggttgag aggtaatcaa ccaattcatt tttacacaga tattataata
     3781 gtttaagaaa gggttttcaa tattttttaa atcgattatg ataatttttt agatcatctt
     3841 tgttgaataa cttcatcctt attcagaact taaagggcac tattataaga atttgaaacc
     3901 gctttactta aatcgtgtat ttttgtacaa taatgattaa ggaggttttt agatgaaaaa
     3961 agaatttacc agtatcctgt ttacagttag tttttgtgga atcatagcat tgcctgtcga
     4021 agctagtggc cctaaagttc cttatacaca agaaggtata acggccattt caaatcaggc
     4081 aacagtaaaa ttgatttcta ttgctgatat cgcgagcagt ttagaaggac aaaaacctat
     4141 aacggttagt tttgatatcg atgatacttt gttatttact agccaatatt ttcaatacgg
     4201 caaagaatat ataactccag gctcatttga ttttttgcat aagcaaaaat tttgggactt
     4261 ggtcgctaaa cggggagatc aggattctat tccaaaagag tatgcaaagc aactaatcgc
     4321 aatgcatcaa aaacgaggag ataaaattgt ctttattact ggccgaactc gaggatcgat
     4381 gtataaaaaa ggtgagattg ataaaacagc taagtcactg gctaaagatt ttaagttaga
     4441 caagccaata gctatcaatt atacaggaga taaagctgtg aagccgtatc agtatgataa
     4501 aacatattat ataaagaaaa acggctcaca aattcattac ggagatagtg atgaagatat
     4561 aaatgctgcc aaagaggcag gtgcaagacc tattcgcatt ttaagagctc ctaattcaac
     4621 taatcttcct ttgccaaaag ctggaggata cggagaagag gttttagaaa attcggctta
     4681 ttagtcaaat atttgtgtaa gaaaggaagt aacagtaatt tctttcatca ccccactgag
     4741 taaccaatag cccagtgggt tttttgggtt acagaaatca gttctaaaaa agaatcactg
     4801 gtaacttaag gttgtctttg atacaatggt aacagtgtat ttaaaagaaa gagaggataa
     4861 catggaaaac cataaaaatg aattcacctt ttccaacaaa tcaattattg cctatgtttg
     4921 gcgtggtgtt atggttggta ttattgctgg tgttattgtt agtttattcc ggttgttaat
     4981 tgaagtgaca gctgactggg tgattgaatg gtaccgatac gctcatatca attcgctgtt
     5041 gctacttcct atactaagtg tcagtttatt agctgttctt tttgtaggtt ttttagtaaa
     5101 gtctgattct gatataaaag gatcagggat cccccatgtc gaaggagaac taaagggact
     5161 aatgtcccca gattggtgga gtgtcctttg gaaaaaattt ttaggaggaa tcatggctat
     5221 ttctatggga tttatgctcg gtcgtgaggg tccttcgatt cagttaggtg ctatgtcagc
     5281 caaaggatta gctaagtttt taaaatcgag tcgtttggaa aagcgagtgc taattgccag
     5341 cggagcagca gcagggctat cagcagcttt taatgctcca atagcaggcc tcttgtttgt
     5401 tgttgaagaa atttaccatc atttttcgcg tttaatttgg attacagcgc tagtggctag
     5461 tttagtggct aattttattt ctctgaatat ttttggattg aaaccaatct tagcaatgtc
     5521 agaggcgatg ccttttttag gactcaatca atattggttg ttgcttttac ttggattatt
     5581 tctaggctgc ttaggctatt tatatgagat agttatcctt aattttaata aactttatgt
     5641 tattttaggg agttggttac atctgccagc ttatttttac ggtattatta tggtttttct
     5701 aattttaccg attggctatt atttaccaca gttacttgga ggagggcatg gtttaatact
     5761 ttctttatcc aatcaacaat tgcctttgat gactatcttc ttttatttta ttattcgatt
     5821 tattgttagt atgtttagtt atggaagtgg cttacctgga ggtatttttt tacctatttt
     5881 aactttaggt gctttggcag gcctattatt tgggcaaata gcttctcaat taggtttatt
     5941 gaaccaatct tttctttctc tttttttgat attagggatg gcagggtatt ttgcggcaat
     6001 ttcaaaagca ccactgacag gcatgatttt ggtaactgaa atggtaggtg atttgaaacc
     6061 cttaatggcc atagctgtag ttacttttgt ctcctattta gtgatggatt tgttaaatgg
     6121 acagccaatt tacgaagcta tgctagataa gatggcaatg aagcatccaa ctaatcttgt
     6181 tgaaccaaca ttaattgaat tgactgttgg tgataagatt gcagggaaat atgttaaaga
     6241 gttaaagctt ccagaaaatg tcttaattac aacccaaatt caccatcaaa aatctcaagt
     6301 tgtgtcaggt aacacgcgcc ttttgtcagg agcaactatc tttttggtcg ttaatgaagc
     6361 tgatacaggt tttgtccgtg aggtattgat gtaggtatta atttatcaca attcggaaag
     6421 ttcgagcttg tcttttagac taagtcttcg aacgttttac tgctagagtt tcatctgaac
     6481 caaagtgaaa cgcttttgat gaagtcaagt aggctttttg ttatactaac aataaagata
     6541 agctaaggag gctgagatgt tagaaattgt ttcagaagag ctacgtcact atcaagaaca
     6601 aaaattgatt gagtaccgta acaaaaatca gttagcccca aaaggaggca ttgtatttgc
     6661 aggtgattct ctcattgaat tttttccttt aaaaaaagct tttggttcat gtttgcctat
     6721 tattaatcga ggtattgctg ggatagatag tcagtggtta ttgcgccatt tttctgtcca
     6781 aattactgat ttagagccta aacatatttt tttattaatt ggttgtaatg atatcgggct
     6841 tggatatgat aaatgtcata ttgtcaagac tattgtagag cttattagcc agattcgtag
     6901 tcattgtgtc tacagtcaga tttatctctt atctttatta ccagtttcca acaatccccg
     6961 atatcagaaa actgtcaaaa taagaacaaa tgctatgatt gatgctatta ataaagattt
     7021 agcaatgatt ccaactatag aatttattaa tcttaatact tgtcttaaag acgaaaaagg
     7081 tggtttatca gatgagaata ctctagatgg tttacatctt aattttccag cttatgctaa
     7141 actagctgaa attattcaat cttatatcta gccaataaat gtactaatag cgatgaggca
     7201 agaagtcaga tagcagatgg ttcatcaatc aattaagaaa aaagttatca agataccatt
     7261 aaaaatagtt gctcaaagtt ctttcaaatc aaaagattat gatatcgcta tgttgagttt
     7321 tagtgtataa tgataattaa aggagttacc atgaaatttg aaaaaaaaca agtcttctat
     7381 ttagtcctta cgtttatctt atgctatggg atcctagcaa actggcgaaa tggaacggct
     7441 atcgtcacga ctatttacaa aacaagcctt cctttttttt atggagcagc aggtgcctat
     7501 attgttaata ttgtaatgag cgcttacgaa aaggtatatg tttatatctt taaagactgg
     7561 tcacatgtct taaaagtaaa acggggtatt tgtttactgt tagcgtattt gacatttttt
     7621 attttaatta cttggattat ttccattgtc attccagact taattacaag tattagtact
     7681 ctaacaaaat ttgatactat aacgatacaa gaagttgtta acaatcttga gcacaataaa
     7741 ctgttagcac gtactattca gtatattggt ggcgatggca aacttacaga gacgattgct
     7801 aactatagtc aacagttatt aaagcaattt cttactgttt tgacaaatat tttaacatct
     7861 gtcacggtta ttgcttctgc aattattaat ctttttatta gttttgtttt ttcgttatac
     7921 gttttagcaa gtaaagaaga tctttgtcgt cagggaaata ctttagtaga tacttatact
     7981 ggtaagtacg ctaaacgcat tcattacttg ttagagttgt tgcatcagcg ttttcacgga
     8041 ttttttgtta gtcagacctt agaagctatg attttaggtt cgctgactgc tagtggcatg
     8101 tttatattaa gattaccatt tgcagggact attggcgttt tagtagcttt tacagcattg
     8161 attccagtta taggtgcttc aattggagca gctattggat ttattttaat tatgactcag
     8221 tccatgtcac aggctattat ttttatcatt tttttaatta ttttgcagca gattgaaggt
     8281 aattttattt atccgaaagt agttggtgga tcgattggat taccggctat gtgggtatta
     8341 atggcaatta caataggcgc ttctttaaag ggaatagttg gtatgattat tgcagttcct
     8401 ttagcagcga ca
//
LOCUS       DAUCWO010000067         7865 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUCWO010000067 DAUCWO010000000
VERSION     DAUCWO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.067"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(41..1567)
                     /locus_tag="VGY20_001471"
     CDS             complement(41..1567)
                     /locus_tag="VGY20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ4274305.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(1560..2288)
                     /locus_tag="VGY20_001472"
     CDS             complement(1560..2288)
                     /locus_tag="VGY20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274306.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPDM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVVNLFGGDEHA"
     gene            2705..3394
                     /locus_tag="VGY20_001473"
     CDS             2705..3394
                     /locus_tag="VGY20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ4274307.1"
                     /translation="MKNAISILALLTPENQLEDTFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLEYLPLQARLSDAKGNILVDNHAFNGSFLPTTDNEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLSQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(3587..4342)
                     /locus_tag="VGY20_001474"
     CDS             complement(3587..4342)
                     /locus_tag="VGY20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ4274308.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKVIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTISYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVTRD"
     gene            complement(4469..5464)
                     /gene="pta"
                     /locus_tag="VGY20_001475"
     CDS             complement(4469..5464)
                     /gene="pta"
                     /locus_tag="VGY20_001475"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ4274309.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(5468..6373)
                     /locus_tag="VGY20_001476"
     CDS             complement(5468..6373)
                     /locus_tag="VGY20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4274310.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(6370..7206)
                     /locus_tag="VGY20_001477"
     CDS             complement(6370..7206)
                     /locus_tag="VGY20_001477"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ4274311.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            complement(7181..7852)
                     /locus_tag="VGY20_001478"
     CDS             complement(7181..7852)
                     /locus_tag="VGY20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ4274312.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
BASE COUNT         2417 a         1695 c         1314 g         2439 t
ORIGIN      
        1 gagacgaaaa aatgccagtc ctacggacca acatttctgt ttatttctgg attaagcctg
       61 ttttcaacag ataatctcga gcgaccttat tagcatctgc tcctttaaca gatacctcgt
      121 agttcatgtc ctgcatctct ttttcagtaa tttttcccgc taattgatta agaatgcctt
      181 ttaattctgg atgttttgtt aataaggaag ttttcatcaa aggagctccc tgataaggtg
      241 gaaacaactg tttatcatcc tttaaaactt tcaaatggta tttcgtaatt tcagcatccg
      301 tagagtaagc atctgttact tggatatctc ctgattgaat tgcttgataa cgaagagctg
      361 gctccatagt cgccacagat agctgtaatc catattgaga ttgcattccc ttatagccat
      421 ctgctctgtc cttaaattcc agagtaaaac cagcctttaa agtatcagca tgcgctttta
      481 aatcagaaat ggtttcaatc tgatattcct tagccaattt ttctggcata gcaacagcat
      541 acgtattttg gtaagcaaat ggcttaagaa gggtcagttt atcttgctta gcaatgcctt
      601 ttttagcatc ctcatagacc tgcttagggt cattagacaa aggtggtttg tcgcgtaaaa
      661 gacttgatgt tatggttcct gtaaattcag gatagatatc aatatctcca gatttaaggg
      721 cttcatataa gaagctagtt ttcccaaaat tagatttcaa ctcaacactt atatctgatt
      781 ggtcttcaat taattcctta tagagattaa tcagaatatc gggctctgct cccaatttac
      841 cggcaataac cactgtatct tttccttttg aaaactgact aagcgccata ggagtatacg
      901 atgctagcaa tgcaagtaag gtgattccaa aactaatcat aatccgtcgc aaggaagctt
      961 tctcaaggta ctgtaacaaa ctattgaaaa taatagctag cagagctgaa gaaatagccc
     1021 ctatcagaat caggttagca ttattacgat cgatccccaa taagatgaaa gagccaagtc
     1081 caccggctcc tatcaaggaa gctaaagtag ctgtgccgat aatcatgact gctgacgttc
     1141 gcacacctga cataatgaca ggcatggcaa ttggaatctc aaacgttttt aaccgctccc
     1201 atttggtcat cccaaaagct attcctgctt ccacaagact tgggtcaata ccatttaatc
     1261 ctgtgatggt gttttgtaaa atcggaaaaa tcgcatagat aactaaagct gtcactgcag
     1321 gcaacgttcc aattcccatt aaagggatga aaagaccaag caaagccagt gagggaatgg
     1381 tttgaaaaac acctgttacc tgtaacataa tgtctgacca acgtttgctg cgactgagaa
     1441 gggctgctaa aggcacacct attagcaagg cgatcataag agataaaagg gaaatttgca
     1501 ggtgttcccc cagagccgcc agccattcat taaaacggtt ttggaaagtt acaaacaaac
     1561 taggcatgct catcacctcc aaataaattg accacaaaag cattagcagg gtgggtctta
     1621 atcatttctg ggcgatccag ttgcacaatt tctccctcat ttaaaatagc tacgcggtct
     1681 cctaatttaa tggcctcatc aatatcatgt gtcacaaata caatcgtcat gtcaaattcc
     1741 ttgtgaagac tcaacattaa ctcttgcaat tgtttacgtg aaataggatc taaggctgaa
     1801 aatggttcat ccatcaatag aattttagga tgagaaatga tagcacgtac aataccaata
     1861 cgttgctgtt cacctcccga taaatcactt gggtaacgat caagataatc tttagcaggt
     1921 aggcccacct tatccaataa ttcttcagtt ttttgtctaa tttcttcagc tgaccattgc
     1981 ttcatatcag ggataatcgc aatattttca gctactgtga gattggggaa aagagcaatt
     2041 tgctgcaaca cgtatccaat agatagcctc atctcacgta aatctaactc tttctgtggg
     2101 acgttattca gtaaaatatc tccagagctt ggttcaataa ggcagttaat catttttaaa
     2161 agtgtggttt tccccgatcc gctggggcca accagcacaa aaaattcacg gtcattaatt
     2221 tgaaaggtct gctcttgcaa aacctttgtc tgaccaaaag ttttagaaac attattaaaa
     2281 cgtatcatta tctctccaat tcattggtta aagtaacagc ataaaagctg atgagttcat
     2341 tcaacctgtc tctctgtcat tttttctcaa aacgttctga gtaactacaa ggaatcactg
     2401 catgccctac catttttaga caaacgcatc tactaattaa agggactctt aggtatcctc
     2461 agtaaggtaa aaactaccaa ttctcaaaaa cattttctct cattattttc ttgtcgtttg
     2521 ttgttgacca ttataaaaag tttcccacta taagtcaaat aatgagacta ttaccccctt
     2581 atagaaaaac aggtcactgg gtcagcctac ctctccaaaa ttaccttatc aaaaatccta
     2641 gacttgccta tgatagataa gcagattagt tgtcttttct agaaaatacg gctatcatac
     2701 aattatgaaa aatgccattt ccattttagc gctcttaaca cctgaaaacc aacttgaaga
     2761 tacgtttatt agagagcttt cccaccactt ctcacacctt tttgaggtga ctattctcac
     2821 tagcaaagcc aacattgagt caaatcaatt gtcgactttc caaggaattt tcagtttcca
     2881 tgagcatgac attgacttgc ctacactcta tttcaaaacc tctcaacatg gtcagggatt
     2941 tttagtgaca gaaagtgtct ttgatcaagc aacagcagtc ttatcgttaa gccaatacct
     3001 cactagattt taccaaaaat ttgatagaca ttttttagaa taccttcctt tgcaagcaag
     3061 gttatctgac gcaaagggga acattctagt cgataatcat gcttttaatg gaagtttttt
     3121 gccaactact gataacgaaa tagaagattg gattcttgct gaattgcgtt tatcagataa
     3181 cccttgtaaa accttcctac ttccttcggg ttcattggat catatttaca tgcagcatta
     3241 tcaagctctt aaaaatcctc aaggtcagct ggtgggagtt ttggataccg tccaagacat
     3301 taaaccgtta ttaagccagt atttagaaga aactggacaa gccattgttg gctggtccga
     3361 tgtgacatca ggtccttcca ttttagatca ctaaacttga ttttaatagg gagtccagcc
     3421 taaatcatca acgcacaaga agcaatcaag aacacttgga aacatcaaaa ccttcttctt
     3481 gactgctttt tttgatgact aaatcggtaa gtctcactta taaagaataa tccattccag
     3541 taaaccattt ttctaaagat tattattctt atggactcat cccttgctag tcacgagtaa
     3601 ctggttgcgg tccataactt tgcgacactg gcatcagctc aatacgatta acattaacat
     3661 gagggggctg atgaatcacc catgatacag tcgacgcaat atcttgaggt tgaatggcat
     3721 gtgctccttg ataaatggcc ttcacccgtg cctcatcacc attaaagcga acctttgaaa
     3781 attctgtccc ttcacataga ccgggttcaa tattggtcac acgtatatgg ctacctgcta
     3841 aatcagctcg taaattgagt gagaactgct taacaaaagc cttgctagcc ccataaatat
     3901 tagctcccgg ataggaaatt gtccctgcgg ttgaccctaa attaataata ataccatcat
     3961 ctttggagac catatgtggt aataattgac gtgtgagata aatcaaccca acaatattag
     4021 tgttaatcat cgtcatccaa ttctcaaaat cggcttcata agccttatca agtcccaatg
     4081 ccaagcctgc attattcact aaaatagtaa tatcttgcca ctgatttggt agacttgtca
     4141 aagcatcatc aatcactttg gtatctgtga catccatctg caaagggtaa aagttgtccg
     4201 acccaaagct cgtttctaag gccttgagtt tctcaagacg acgagaagca ccaataacct
     4261 ttatcccatc tgcaattaag tgttcgacaa tggctctgcc aaaaccggct gaagcccctg
     4321 ttactaaggc aatatttccc atacatttcc tcaccttcaa aaaaaacgag atggtctggc
     4381 caactcgttt ttaataatgt caattagtct taggacaact tctaacacat cacttagcgg
     4441 ttaggatcct atatcaaggg gtcttaagtt acccttggct ttcaatagct tgtgctgctg
     4501 taataatagc taatttgtaa atatcttcag cgcttgagcc acgagataaa tcattaacag
     4561 gtttgttcaa tccttgtaag attggtccaa tagcatcaaa cattcctaaa cgttgtgcaa
     4621 ttttgtaacc aatatttcca gattgtaagt cagggaaaat aaaagtatta gcttgaccag
     4681 caactgctga atctggtgct ttgatagctg ctgtttcagg aacaaaggct gcatcaaatt
     4741 gcaactcacc atcaagtgcc aaatcaggat tcaacccttt agcaatttct gttgcttctc
     4801 ttaccttatc aacttgaggc gctttaccag aaccttttgt tgagaagctt aacatagcaa
     4861 ttttaggatc aatatcaaag attttagctg tttcagctgt gttaacagcg atttctgcca
     4921 attcttgtgc tgttgggtca atattgatag cacaatcagc aaacacgtaa cgctcactcg
     4981 tattttcgcg attcatcaag aaaacacctg aagttcttga aatacctggt ttggttttaa
     5041 tgatttgaag ggctggacga acagtatcag ctgttgaatg aatagcacca gataccattc
     5101 catctgctaa tcccatttta accaacataa caccaaagta attcacatca cgaagcattt
     5161 tatcagcatc ttcaagcgtt gcttttcctt tacggatgtc aacaaaagct tctttcattt
     5221 tgtcaaaatc cgcatattca tttggattaa tgatagtata gtcttgatca gcgaaaccta
     5281 attttgtcaa aaggttgcgt acctcttctg actgtccaag gataatcggt tctaagagtc
     5341 cttcaaattt caaacgagca gctgcacgga cgacgcgctc atcattacct tctgggaaaa
     5401 caatcttcat gtttttacca agaatttttt ctcttaaacc accgaataaa ctacgaatac
     5461 tcattcttta ataacctcta tcttttttct gtaaacctat gataacgcta tcaaagtcat
     5521 ctgtcaagtg tattgcatag ctacaatcac tttctgtaaa tggatctttg aaattcaaat
     5581 aatgacaatg taaagcttgc cttgttattc ctaagtccaa acgacctccg taaaggtcat
     5641 cccctaaaag tggaaaacca atatgggcaa aatgaactct gatttgatgg gtacgtcctg
     5701 tatgcaattt gatatccact aaatgcacat tttctgaata gcgcgccaca accttatagc
     5761 tagtcttggc atatttgccc atagggtcaa ctgccctggt aataatacta tcttttgaac
     5821 gcccaattgg agcaataata tctccttcat ctggcaacgt cccatttcca gaaaccagag
     5881 caaaataacg tttctctatt gagcgagttt ggagttgctt gtccaacctt gcatgagcat
     5941 aaccgtgctt agcaaataac atcaagccac tcgtatctct gtctaaacga gtcacgatat
     6001 gcacttgttg gtctaggtaa tgattgtcca cataatatgc cttgataaag ttagcaatgg
     6061 tattggagtg gatggcactt ggaatactgg caaagccaat gggtttatta atcaccaaaa
     6121 aatgatcatc ctcgtgaaca atatccaaat caaaagggat ggcttcaagc ttttcaaagg
     6181 gctcttcgtt tggaatgtca atggtgacaa catctcccac ttgcaataaa taaatggcat
     6241 tttgttcaat accattcact aatatattac cgcccttata tttgatttta gccaatagtc
     6301 ctttagagac atcatgactt tttaaaaggg tttttacttt tattcgttta tctgctacaa
     6361 attcaaatct catgagtcaa tctctccaat aaaggcatcc ttgacccttt cccaaaagct
     6421 cgtatgactc ggagaggaaa caaaatgaat tttctcatcg tcgataaaat actccacctt
     6481 cgtcacattt tttaactgat aggttttatt atcaatggaa atggtataaa ttcctaatcg
     6541 ttttggcact aactcaatct tatctttttt gggaataatg attgatgagc ccaaggttct
     6601 aaagacacgg ttattaagac tggaaatttc cgtcaattgc agcgcttcaa tcgtcggatg
     6661 caagacagca ccacctaaag atttattgta ggctgtgctc cctgtcgggg tcgatactga
     6721 aatcccatca cctcggaagc tttcaaattt gacatggtta ataataacat ctgctaccat
     6781 cgttttttca atacgcttaa ccgtcgcttc attcaaagca cgcgctttaa ccacacgacc
     6841 atcatctaaa gtaatagcaa cttttaaaat aggataagag atttgttctc ccttgtcttt
     6901 tcttaaatta tcaatcaatt tatcaacttc aaaatcccta taatcggtat aaaagccaag
     6961 atgaccggtg tggattccta caaaacgtac cttatctaat tctttttcat acatgtgaaa
     7021 agcagataag agcatcccat ctccgccaat agaaatcaca atatccggat ttttctttga
     7081 aagatagaaa tcaggatcat ctttaaatac ggagaaaagt ttggaggcaa cgcgtttact
     7141 ttggtactta ccatttgcaa taatagcaac tcgctttacc ttacctgtat aattcatctg
     7201 tgtcatcact atttcctaca ccatcactta aatttcgtgt cacggggtca aacaataatt
     7261 gagcctcccg aatatcttct cgaatcttac gcatttcttc gtctaattct aaggcaattt
     7321 tagcagtcac ttcaagacgc tttttaattt cttctgggaa gtctccacca tatttataat
     7381 ttaaagaatg ctcaatggtc gcccaaaagt tcattgccaa agtacgaatc tgaatctccg
     7441 ctaagacctt tttttgaccc tcaatggtat caacagggta ctcaaccacc acatgataag
     7501 acctgtagcc actggacttc atattgcgaa tataatctcg ttcataaaca attgtcatgt
     7561 cttgtcgttg acgcaataga gctaagactt cttccacatc atcaacaaac tgaaccataa
     7621 tacgtagtcc agcaatatct tgaatatctt gtgctatatt ttcttcaata acaccacgta
     7681 aaatcatttt ttctttaata ctttcaattg acttaacacg tcccgtaaca aattcaatcg
     7741 gagaataacg attttgctta cgatattgct tgcgaatacc ccgtagtttt atttttaatt
     7801 caccaactgt ttggatataa ggatctaaaa attcttccca atctaaggtc atctattgtc
     7861 tcctt
//
LOCUS       DAUCWO010000068         7558 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUCWO010000068 DAUCWO010000000
VERSION     DAUCWO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.068"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..526)
                     /gene="speG"
                     /locus_tag="VGY20_001479"
     CDS             complement(<1..526)
                     /gene="speG"
                     /locus_tag="VGY20_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEQ4274313.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     QPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSES"
     gene            complement(628..725)
                     /gene="ffs"
                     /locus_tag="VGY20_001480"
     ncRNA           complement(628..725)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGY20_001480"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            925..2157
                     /locus_tag="VGY20_001481"
     CDS             925..2157
                     /locus_tag="VGY20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ4274314.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQSQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2771..3286)
                     /locus_tag="VGY20_001482"
     CDS             complement(2771..3286)
                     /locus_tag="VGY20_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ4274315.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(3286..4065)
                     /locus_tag="VGY20_001483"
     CDS             complement(3286..4065)
                     /locus_tag="VGY20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4274316.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQARVKKLVLTHMPPVP
                     PEGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(4205..4744)
                     /locus_tag="VGY20_001484"
     CDS             complement(4205..4744)
                     /locus_tag="VGY20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ4274317.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(4748..5059)
                     /locus_tag="VGY20_001485"
     CDS             complement(4748..5059)
                     /locus_tag="VGY20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274318.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQEISPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(5276..6418)
                     /gene="tgt"
                     /locus_tag="VGY20_001486"
     CDS             complement(5276..6418)
                     /gene="tgt"
                     /locus_tag="VGY20_001486"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ4274319.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6637..7500
                     /locus_tag="VGY20_001487"
     CDS             6637..7500
                     /locus_tag="VGY20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ4274320.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGVILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     ANLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIKTSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
BASE COUNT         2361 a         1563 c         1332 g         2302 t
ORIGIN      
        1 gcgattccga atcatagata ttgtattttt ccattagaaa ttttcttatt ttaaaatcaa
       61 attcctgtaa agtaaactgt ttctttttta tgcgaacagc ctcagagggc aaataagttt
      121 ctttcccatc ttgatttaaa attcctacaa actgtaatga tttatttcct tgattcgaat
      181 gttttacaat accaccataa atattatcta cataaggttg ggaaaaatta taagggagac
      241 caaaaacatc gaccttatcc ccttttataa actgatcagt aatgcctaaa gatacaatag
      301 aatcaacata aagaatacat tccgatcttt tttgtttagt attaatatgt atgctatttg
      361 tagtaacaaa tgcaatttct acattttcat aatcgcatgg ggtaatatta taggcaaacc
      421 ttaaacttct ttttaaatct tttaaatttt catctgcata agctaattga cttccatagc
      481 taaaaacaca tgataatatg ataattgtca aaatgtttgt tttcatttaa aactttttat
      541 gtcctgtttt atacctctta acctaaataa aaatatcaat cggtttcaac aactgatgac
      601 actatagaag gaggagatta ctttagtaaa gtcaacaaaa aagagcacac acccgaaaat
      661 cgcttagggc tgctggattc ctcccctgac ccacttcacg cacaagtgtt gctccgccta
      721 ttattatatc atattacgga cagtttgcaa gttttccaac taagtaaaat cactttcaag
      781 tgagaatagc ttcattttgg gacaagatgg actgaatacc tagaaaaatc atgacccttt
      841 ccaaacacga ttttgctttt tgaaaatctt tccctctccc cattccagct attctctatg
      901 ttataataag attattagat tagaatggga gttatgatga aacaaaagat taaaatctta
      961 acagtaattg gccttatgac agttggtatg tctgcgtgtc acaacactag caaaccttct
     1021 aataccgatt ctgttttttc tttaaccgga aaaaagcgcc aacaaatcgt caaacaagta
     1081 agacaacggt attatttcca acaattatcc aaaaccgaac aagaaaatta cctcacttta
     1141 tacgatagtc tagcacaatt tcgtgaaatc atcagtttga cgccggcttc taaaaaaagc
     1201 ttaattaaaa cgattgacgc ctttgtgatg gataatcccg aattttactg gattacatct
     1261 gccgattatc gatttgagtt ttctgatcaa acggtctttg tcacctttcc tatcccagaa
     1321 gatgccaaga acgtttatca agacttgcaa gcaatcggta atgatatcgt cgcaaacacg
     1381 ccatcaaagg atcgctatga gcaggtcaaa tatttttatg aggtcatcat tcgagacaca
     1441 gactacaata aaaaagcctt tgaagcctat caatcaggca gtcaatccca ggtcgcttct
     1501 aatcaagaca ttaaaagtgt ttttattgat catttatctg tttgtaatgg ctatgcccaa
     1561 gcctttcagt ttctttgtca aaaagctggc attccagtcg cctatatccg tggaactggt
     1621 acatctcagc aacctcagca atcttttgca catgcatgga atgctgttca aattaataac
     1681 acttattatg gcgttgatgt cacttggggt gaccctgttt ttgataacca tctctcacat
     1741 caaaagcaag gaactatcaa ttacagtttt ctatgcctac cagattatct aatggcctta
     1801 tcacatcagc caagcaaaga cattgctttt aatactaaag aacgttttga aaatgtttgg
     1861 actatacctt cttgtacaga tgattctttg ctttattcta aacgtcatca aagctatatc
     1921 tcaacgtttg acagtgacgc catccttgca agccttgaaa atcagttgtt aaatagacaa
     1981 gagcaactgt ctctacaatt tgctcatcaa gatgattatc aacaaatggt aactgattta
     2041 acgacgaatc aaacaggtta tcataacctt ttcaatcagt attggaataa ttatacgggc
     2101 tttacctatg gactcttacc agagactctt agtatttcat ttgctagtag gaactaggtt
     2161 tagacacatg aaacacaaag ttaagatgat tatctttgtc aaacattaca agaaattgat
     2221 atactaacac aattgaaggc gattattatt ctaaccgtct tcaatagata taaccttttc
     2281 agattgagca catctaatgc ttgtaacatt agcaacagtg atgagctctt ttttaatgct
     2341 ttagcaaaat atatcagcta agacaaaaga ttaaataact caagtcatgc ctataagtca
     2401 cttgttttta atccctgtgt cagtgtcaat ctacgccaca gataactgac agctctgagc
     2461 cctagaagtc tgaacttgat aagtttacta aaaacaaaaa taaggtgagc cattactatc
     2521 tgtttttaga tagtaaatct ttttaagaat gactaactca tctagtcatc acgtgtttta
     2581 tttttcgatt gttttcatct cgattgggtc tgaaaaagag cgcttctagt acttggaaac
     2641 gcccttttgt atattctatc ttcgtatgtt gaatctaaca accattggaa aaatggtctg
     2701 acgtttcact cagtattttg acaggctacc cttcaccaaa aatgcatgtg ataacaaact
     2761 ccttaaaact ctagtcaaag ggatctgact gttccttgat caaatgctta gctatttttt
     2821 tgcgctctcg cccttggcgg aaaaaggttt gcatgatgtt agcacagtct gctgctagca
     2881 ggcctcgttc aacttgcacg cggtggttta agcgctcatc tgtcaaaatc tggtaaaggc
     2941 tgtctgctcc accaaatttt tgattgctgg ctccataaat aacatgcgga atccttgcta
     3001 gcccaatggc accactacac atcacacaag gctcgatggt cacaaagagt gtggtgtcta
     3061 ataaacgcca gttgccttca tgagcgttag cctcgttaat agccatcatt tcagcgtgca
     3121 taatggcttg gttactctct tcacgcgcat tgtgcccacg accaataatc tcaccatcct
     3181 tgacaatgac acagccaatg gggatttcag ctttctgtaa ggatttttcg gcttctttta
     3241 aagcctcttg catgaaataa gtttgctctt ctaaactata tggcatcaag aaagactccc
     3301 caagttccaa gatttgtgtg gtaaggcaag gtcaacagga atattacctg cataggcctg
     3361 tgtttcttgc ctcaacactt ctaaatggtt tttggaatta ataccttctg gaggaaccgg
     3421 tggcatgtgg gtcaagacaa gcttcttaac acgtgcctgg ctagccatta gtcctgcttc
     3481 cttactggtc aaatgggcca tatgattttc attgccttcg tataagtaaa catccgctaa
     3541 gaaaaggtct gcttcttttg cgaagtctgc caatccttca aaataaccac tgtctccagt
     3601 aaagacaagt acctgaccag tcgctctttc tacaattcga aaggcataac acacaactgg
     3661 atgaactgtt ttaagaaagg taatgtcaaa aggaccaatc tgttcaactc cagtaacatc
     3721 ataggctcta ccttctgaaa catcaggaat gcttaactta gcaaattcgt gtcggtcctc
     3781 atcatgccca taaatgggca atactttagg ctgccacagg tgtttaggat aaagttgaaa
     3841 ataatggcgc aacacgccta aatcggccac gtggtctgga tggtaatggc taataatcac
     3901 tgcatccaaa tcgagaggac tgatttcttt ttccagctca ttgagcgctc gactaccagc
     3961 atccatcaat aactgaaagc cgtcatgtcc tgtgaccaaa taagaggttg tgcctgcatc
     4021 ttgataggga taggctcccc aagaccctaa ggtggttaat ttcatctcaa acccttcttt
     4081 cttgctttaa ctcttattat aacaaaaatt agtgtcaaag aggattgaga cccaccaaaa
     4141 acaggtccta gcagataaat ctgccaagac ctgttcaaca caacttaact atttgatgat
     4201 tagattactg ttttgtattc aaaaaactat ctttgtattt aacagcgata atggtcgcaa
     4261 tgattgcttt gatggtatct cctggaataa agaccaagtt tgacaaaagc gatgtcacaa
     4321 gagacatgcc agtgtaggct gccaaccaga tagcacccag aacatcaacc agtacaactc
     4381 ctgagattaa aagagctagg aatgctaatg gcgtgctttt tgagaaccag ttgcggtaaa
     4441 gtaatgaaaa gacaataggc acaagaaggt aggcaataac atagccagca gatggcccca
     4501 tcaatactgg aatggttgtt cttgagcctg agaaaacagg taagaaaagg ccaatcacta
     4561 aaaataagaa aacagataag gttccctttt taccacccag catcaatcca gccaacatga
     4621 cacctaagtt ttgtaaaaca atagggactg ggataaagcc tagcgggata gcaggaataa
     4681 agcctaaaat aataatcaaa gtggtcatca tagccacctt aacaagttct ttggttgtaa
     4741 acatcagtta cgactccttt aaaaaataga tatccttgtg cctatgacag gctggattaa
     4801 agggactttg acaataagga cagcatccac aaccatactc agctaggctc aagagctttc
     4861 gacaatgccc acaaataaca ggagatattt cttgatgtcc tgttggcaca aaggcatgat
     4921 ctgttaactg gtcatggcaa tggtaacaag cataataagt ctgacaaaaa gcacatttga
     4981 gcccaacaat atcaagcggg gtatgataat gtaggcaccg atactcttgg tcaaggtcaa
     5041 ttccaaaaca gtccgtcata tctcatcctc ttgtaatagt ttaatcaaaa gcttatagtt
     5101 tgtcaactct atttttacaa aagggtaact gctttctgaa aaagaacacg taaaacaaga
     5161 acttcccttt aaagagtgac ctatcagttg aaagcaatag gacatccgtc tatacaaaaa
     5221 aactgaaacc aaagacgata gatcgccctc agttccagga tcatagcatt tttaattaga
     5281 aattacggtt tgatttatta taaccatacc gttcaagaaa atcttggcgg aattcaagta
     5341 agttatcgtc cataatagcc tgacgtactt ttttcatcaa gttaaccaag aaataaaggt
     5401 tgtggtagct tgtcaaacga ataccaaaag tttcatccgc ttttagcaag tgacgaatgt
     5461 aggcacggct atagttttga caagtgtagc agtcacagtc atgatcaagt ggggtaaaat
     5521 cttcagcgaa tttggcattt ttaaccacaa gacgcccctc actagtcatg caggtcccat
     5581 tacgagcgat acgcgttggc aagacacaat caaacatatc gacaccacga atcacaccat
     5641 caatcaagct atctggtgct cccactccca tcaggtaacg aggtttattt tctggtaaga
     5701 ggggagtcgt aaagtcaagg acggcattca tttcttcatg gctctcccca acagctaaac
     5761 caccaataga ataacctggg aaatccatag ccaccaaatc tgccgcagac tgacgacgaa
     5821 ggtcctcaaa tccagctcct tgcacaattc caaataatcc ttggtcatga ggacgacgat
     5881 gggcttttag gccacgttct gcccaacggc tagtacgttc aatggatttt ttcacgtaat
     5941 cataaggttg gtaaaattgc gggcactcgt caaaactcat catgatgtct gagccaaggt
     6001 tattctgaat ggaaatggct ttttcgggtg atagaaacat ttttgagcca ttcaagtgat
     6061 ttttgaaagt cactccttct tcagtgatgt ttcgtgaatc ggctaaagag taaacttgaa
     6121 aaccacccga atcggttaag attggctgat cccagttcat aaacttgtgc agaccaccag
     6181 agcgagcaat caactcatct cctggacgca accagaggtg ataagtgttt gataaaataa
     6241 tccctgaccc aatagctttt aactcttctg gagactgtgt tttaacagta gcctgagttc
     6301 ccactggcat aaacattggt gtcggaaagg tcccgtgagg tgtgataatt tcgcccaagc
     6361 gtgcaccggt gtgcttttct gttttgataa ggcgatactt aatcggataa tctgtcatgt
     6421 gctttcttcc ttgctatcaa gataaacagt cttgagcttt tttgatctgc ctgagtttcc
     6481 atcataatga agggcttcaa gatgacatgt tgttctagcg cctatcattt tatcaaaaaa
     6541 tccataattt gtcatgcttt cccaaaaaag tatagtcaaa ataatggtta gctgtctcat
     6601 tttgttttat ggtataatga aataaaggag tctgttatgt caattaaagc aatcaaaggc
     6661 caggctagag acacgttaaa aaatctctct ggcaagtatt tactattttt aattccaacc
     6721 cttctcttta tgtttcattt cggtattgag attcatcaag gttacgtcct atctagtggt
     6781 attgaagtct cgctagcggc tagttatttc ccattactac taggagttat ccttagcctt
     6841 ttcattttat cagcatcctt tacaatgatt gatgttgtta ggcattttcg tcagaaagtt
     6901 tcttttgccg aatcaacaac agccttctca aaagaattct ttgcgaacct gctggtttta
     6961 gcgatcacta agtggctatt ctttctcatc tggtcactaa tttggttttt tggcctcttt
     7021 attttccttt ccggactttc agcgtttttg gtcaatgcta aatcaggtag ctcaacagtc
     7081 atttcactga tctttttact atttggagca gttttgtcct taattggttt tggtatttat
     7141 atcaaccgtt attacgctta tagtctatca gaataccttc tctacgataa ggttaaagag
     7201 gggacttatc taggagctat agccgtcatt aaaacaagtg tagctatgat gaaaggttat
     7261 aaatggaagc tttttttcct acaacttagc ttcaccggtt ggtttctact gaatattgtt
     7321 acttttgggt tacttaatat ctatctttta ccatatttta caacagccaa tgtcattttt
     7381 tatgatcaac tcaagaaacg tttcaaagac aaagatgacc caattgaagg agaacattta
     7441 agccttcatc agcttaataa aaagcctact cctatgatgt atgacgatca aaaagactaa
     7501 ataaatcctc cgtccataaa aatagccctt gaactgcacc ccaaaagtta gacacaaa
//
LOCUS       DAUCWO010000069         7529 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUCWO010000069 DAUCWO010000000
VERSION     DAUCWO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.069"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            25..732
                     /locus_tag="VGY20_001488"
     CDS             25..732
                     /locus_tag="VGY20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ4274321.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEIFR
                     LLRVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            795..1850
                     /gene="prsA"
                     /locus_tag="VGY20_001489"
     CDS             795..1850
                     /gene="prsA"
                     /locus_tag="VGY20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ4274322.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AAEPTETEAQTQEPAAQ"
     gene            2237..4855
                     /gene="alaS"
                     /locus_tag="VGY20_001490"
     CDS             2237..4855
                     /gene="alaS"
                     /locus_tag="VGY20_001490"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ4274323.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRN
                     EAIGWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAEIV"
     gene            5216..5431
                     /locus_tag="VGY20_001491"
     CDS             5216..5431
                     /locus_tag="VGY20_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274324.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            5428..6189
                     /locus_tag="VGY20_001492"
     CDS             5428..6189
                     /locus_tag="VGY20_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ4274325.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEENDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            6208..6903
                     /locus_tag="VGY20_001493"
     CDS             6208..6903
                     /locus_tag="VGY20_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ4274326.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIVGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(7001..7231)
                     /locus_tag="VGY20_001494"
                     /pseudo
     CDS             complement(7001..7231)
                     /locus_tag="VGY20_001494"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7256..>7529)
                     /locus_tag="VGY20_001495"
     CDS             complement(7256..>7529)
                     /locus_tag="VGY20_001495"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEQ4274327.1"
                     /translation="TRPNLVNQCFQATGKNKLWLGDLTYIPTQEGILYVSVLIDVYSR
                     KIVGWAMGRRMQDKLVTEAFNQAYNREKPKEGVIVHTDQGSQYTGA"
BASE COUNT         2380 a         1323 c         1564 g         2262 t
ORIGIN      
        1 gggttttttt atataattag gtttatggtt aaatcataca gtaaaacagc aaatcataat
       61 atgcgtcgtc ctgttgtcaa agaagagctt gttcattata tgcgaacacg gcagaagcaa
      121 accacaggtt ttttagcaga gttagagcaa ttcgcaagac aagaaaacat tcctattatt
      181 cagcctgagg tcgtggctta tttcaggttt ttattgcaaa gcttacagcc aaaacacatt
      241 ttggagattg gcacagccat tggtttttct gcgcttttaa tggctgaaaa tgcaccagat
      301 gcgacgattg tgactattga tcgtaatcga gagatgatcg attttgctaa agcaaatttt
      361 gctaagtacg acagtcgcca acagatccgc ctgttagaag gagatgcagc agatatatta
      421 tctacacttg aaggaaattt tgactttgtg tttatggatt ccgccaaatc caaatatatt
      481 gtctttctac cagagatttt cagactgtta agagtcggtg gtgtagtcat tttggatgat
      541 gtctttcaag gaggtgacat tacaaaaccg atcgaagaca ttcgacgagg tcagcgtact
      601 atttatcgtg gcttacagtc tttgtttgat gccaccttaa ctcatccaaa cctaacaact
      661 agcctggtcc ctcttagtga tggattatta atgattcgta aaaatcaagc agatattgta
      721 ttaccagatt aagtaacatt gataaattta tgttataata aattagttaa agataaaaag
      781 gagctcagaa gtaaatgaaa aactcaaata aactcattgc tagtgttgtg acattggcct
      841 cagtgatggc tttagcagct tgtcaatcaa ctaatgacaa tactaaggtt atttcgatga
      901 aaggtgatac aattagcgtt agtgattttt acaatgaaac aaaaaacaca gaagtatcgc
      961 aaaaagcgat gctaaatctg gtaattagtc gtgtttttga agctcaatat ggtgataagg
     1021 tttcaaaaaa agaagttgaa aaggcgtatc ataaaacagc tgaacagtat ggcgcttcat
     1081 tctctgctgc tttggcacaa tcaagcttga cacctgagac ttttaagcgt cagatccgct
     1141 cttcaaaatt agtagaatat gcggttaaag aagcagctaa aaaagaattg acaacacaag
     1201 aatataagaa agcatatgaa tcttatactc caacaatggc agtcgaaatg attactttag
     1261 ataatgaaga gacagctaaa tcagtcttag aggaactaaa agccgaaggc gcagacttta
     1321 cagctattgc taaagaaaaa acaacaacac ctgagaaaaa agtgacctat aaatttgatt
     1381 caggcgcgac aaatgtaccg actgatgtcg taaaagcggc ttcaagtttg aatgagggtg
     1441 gcatatcaga cgttatctcg gttttagatc caacttctta tcaaaagaag ttttacattg
     1501 ttaaggtgac taaaaaagca gaaaaaaaat cagattggca agaatataag aaacgtttga
     1561 aagctatcat tatagctgaa aaatcaaaag atatgaattt ccaaaacaag gttattgcaa
     1621 atgcattgga taaagctaat gtaaaaatta aagacaaagc ttttgctaat attttggcgc
     1681 aatatgcaaa tcttggtcaa aaaactaaag ctgcaagtga aagttcaaca accagcgaat
     1741 catcaaaagc tgcagaagag aacccatcag aatcagagca aacacagaca tcatcagctg
     1801 cagaaccaac tgagactgag gctcagacgc aagagccagc tgcacaataa gtaggatttt
     1861 attgactaat cagttaaaaa aacttataat aatttagtta agaattgttt atcaaggatt
     1921 agatacagaa aagtgacggt tgctgcgagt cattgataat tagataaaca tgctacttaa
     1981 ctttacaaca attgatatcg taaattaaga atgacaagtt cattgaatag aggtggtacc
     2041 gcggttttcg ccctctgtga gatggacttg ttttgtattg aggactattt gaaatcaacg
     2101 aaaggaatag agtagactag gctgccaggc ttatagctga tctcctagca agaaagacga
     2161 gctgtgtcaa ctggtgtgat tacattacta gcttcagcat aacactgttt ttcttactct
     2221 ttgtatctaa atttttatga aagaattatc gtctgcacaa atccgccaaa tgtggttgga
     2281 tttctggaaa tctaaaggac attgcgttga gccttcagct aacttggttc ctgtgaatga
     2341 cccaacgctt ctttggatca actcaggtgt tgcaaccttg aaaaaatact ttgatggttc
     2401 agtgattcca gaaaatccac gtattaccaa tgcacaaaaa tcaattcgta ctaatgatat
     2461 tgaaaatgtt ggtaaaacag cacgtcacca tactatgttt gaaatgcttg gtaacttctc
     2521 aattggagac tatttccgta atgaagctat tgggtgggga tttgaactct tgacaagtcc
     2581 tgaatggttt gactttccta aggacaagct ctacatgact tattacccag atgacaagga
     2641 ttcgtataac cgttggattg cttgtggcgt tgaaccaagt cacttggtgc cgatcgagga
     2701 taacttctgg gaaatcggtg ctggtccttc aggtccagat acggagattt tcttcgatcg
     2761 tggtgaagat ttcgatccag aaaatatcgg acttcgcctc ttggctgaag atatcgaaaa
     2821 cgatcgttac atcgaaatct ggaacatcgt tctctcacaa ttcaatgctg acccagccgt
     2881 accacgttca gaatacaaag aattaccaaa caaaaacatt gatacaggtg ctggtcttga
     2941 acgtcttgca gctgttatgc aaggggcaaa aacaaacttt gaaactgacc ttttcatgcc
     3001 aatcatccgt gaagtagaga agttgtcagg taaaacttat aatccagatg gcgacaacat
     3061 gagtttcaag gttatcgctg accacatccg tgcgctttca tttgctatcg gtgatggtgc
     3121 gcttcctgga aatgaaggtc gtggttacgt tcttcgtcgt cttctccgtc gtgcggttat
     3181 gcatggccgc cgtcttggca tcaacgaaac tttcctttac aaattggttc cgactgttgg
     3241 acaaatcatg gaaagctact acccagaagt gcttgaaaaa cgtgatttta tcgagaaaat
     3301 cgttaaacgt gaggaagaaa catttgctcg tactatcgat gcaggtagcg gtcacttaga
     3361 ttcattgctt gcgcagctta aggctgaagg taaggatact cttgaaggta aagatatctt
     3421 caaactttat gatacttatg gattcccggt tgaattgaca gaggaattgg cagaagatgc
     3481 aggctacaag attgaccacg aaggctttaa gtcagccatg aaagaacaac aagaccgtgc
     3541 gcgtgcagct gttgttaagg gtggttcaat ggggatgcaa aatgaaaccc tagctggtat
     3601 tgttgaagaa tcacgattcg aatacgacac atatagtctt gaatcaagtc tttcagtcat
     3661 catcgctgat aatgaacgta ccgaagctgt ttcagaaggt caagcccttc ttgtctttgc
     3721 tcaaacacca ttctatgctg aaatgggtgg acaggttgct gacacaggta gaatcaaaaa
     3781 tgataagggt gacacagttg ctgaggttgt tgatgttcaa aaagcaccaa atggtcaacc
     3841 tctacacact gtaaacgttt tagcatcact ttcagttgga acaaactaca cacttgaaat
     3901 caacaaagag cgtcgtttgg ctgttgagaa aaaccacaca gctactcact tgctccatgc
     3961 agctcttcac aatgttatcg gtgaacacgc aactcaggct ggttcattga acgaagaaga
     4021 attcttgcgc tttgatttta ctcactttga agcagtaagc aatgaggaac ttcgtcacat
     4081 tgaacaagaa gttaatgagc aaatttggaa cgctcttaca atcacaacga ctgaaactga
     4141 cgttgaaacc gcaaaagaga tgggagcaat ggcgcttttt ggtgagaaat atggtaaagt
     4201 ggttcgtgtg gttcaaattg gtaattattc tgttgaactt tgtggtggaa ctcacttaaa
     4261 taattcttca gaaatcggtc tcttcaagat tgtcaaagaa gaaggtattg gttcaggcac
     4321 tcgtcgtatt attgcagtta ctggtagaca agcttttgaa gcttatcgta accaagagga
     4381 tgccctaaaa gagatcgctg ctactgtaaa agctccgcaa ttgaaagatg cagcagctaa
     4441 agtacaagct cttagcgact cgcttcgtga tcttcaaaaa gaaaatgcag aacttaaaga
     4501 aaaagcagca gctgcagcag ctggtgatgt ctttaaagat gttcaagaag ctaagggcgt
     4561 gcgcttcatt gctagtcaag ttgatgttgc agatgcaggg gcacttcgta catttgctga
     4621 taactggaaa caaaaagact actctgatgt gcttgttctc gtagcagcta ttggtgagaa
     4681 ggttaatgtc cttgttgcaa gcaaaaccaa agatgtccac gctggtaaca tgatcaaaga
     4741 attggcacca attgtagcag gtcgtggtgg aggtaaacca gacatggcta tggcaggtgg
     4801 tagcgatgca agtaaaattg cagagctgct agcagcagtt gctgaaatag tgtaaatcca
     4861 taaaaaacca agttatttag aacttggttt tttatatttg aaatgactac catcataacc
     4921 tttttgacta ttatcgttgt caaaaagaaa aagatatgtt acaattatag taacaaatag
     4981 acatgaggag gtgttagaga aaatgacaca tggtatcaaa cttaagaggc agatttgctt
     5041 atgggcaagt atcctgactc ttttcatttt aagttatcat cttatcaggg taatctggtt
     5101 tggagtaccc ttgagttatc tggttttggg ctttacagga ctagccttgc tgttgaatac
     5161 tttttcttat tttagaaagt aagttataag ttaaagaaga atgaaggaga tggcaatgga
     5221 ggttattctt aaaaatcggc tcaaagaatt acgagccaga gatggaatta atcaaacgga
     5281 aatggctaaa ctggcaggag tatcgaggca gaccatcagt ctcattgagc gtaacgagta
     5341 caccccatca gttataattg ccatgaaaat tgcaaaggta tttcaggaac cagttgaaga
     5401 ggtgtttcgt ctagtggagg tcgaggaatg aaaaagaaga agaatagttt tttacgcttg
     5461 ttaaaaatgt tattgctatc aagtttggcg ggagggatta taggtggcat ggttggagcc
     5521 tttttaggtt accatggaga gagattagat cagctcacgt tcctaaaaga tgatgtgatt
     5581 aacctgatta tcttattaaa tcgtctagta gttgtcacag gcctcacact ttcttttgtt
     5641 tttttaacgc aactgaaaaa agagacagct gtttataaca caattgaaga gaatgattat
     5701 tctgaaaatg gttaccgtca gctgaataaa aagcatgctt ataccatgtt attgattgcc
     5761 gtagcttcta ttttatccat gtgcaatgtg ctacttggtc tgaccttaac taatgattca
     5821 cagcatgcta tgctggccat tccgcttctg gatatccttt tattattaat ggtcattcct
     5881 tttcaagctt tagccatgaa acgttacaat gccattcggg gaactgatgt tccttatttt
     5941 ccaaatttga aagaattaaa acataatatc atggctctag atgaggcaga gttacaagcc
     6001 tatcataaga catcgtttga aagtgtgttg tcattaaatg gagtcatcat cccttccttg
     6061 tatgtgattt tattttttgt ctatctattc actggtcaag tggagttaac agccatctta
     6121 gttttggtgt taattcagct ctatttactc gttaagagtg caacaatgac cagacaattt
     6181 taccgttagc ctaaaaggag actgtttatg aaaggattca ttaattattt aaaaatagca
     6241 gtgctcatta ttctggctat ggttttcaat gtccttccga tgattttatt gcaaaagcaa
     6301 cacgatattc ctatggtgct taattgggga attggtattt tctacttggt tattgttgga
     6361 agtgtcctta ttgtattatg gcgtctttat caagctaagc aagacacccc tattaaacag
     6421 caaaaaatga gattggttga ctggggttat ttagcattat tttggttaat catccgtgtg
     6481 atagctattg taggtaccct tgttaaccag ctatggtctg gtcaacaagt gagtgctaat
     6541 gatgctgcaa tacatacctt agctagactt atcaaaggtg gtttcccgct ttatactgcc
     6601 ctatttgtac ttgtgatagc ttttatcgct cctattatgg aagaactagt ctttagagga
     6661 tttcctatga ttgatctctt caaaggaaaa tcacttaagg tggcaggttt agtgacctct
     6721 cttgtttttg ctttaccaca tgccaccaat agtgttgaat ttatcatgta cagctgtatg
     6781 ggcatttttc tctttgttgc ctatcaaaga cgaggaaact taaaagacgc tatcttgtta
     6841 catattttta ataacttgat tgaagtcatt ttgttaatgt caataggctt gggagtcata
     6901 taataaaaag cttcgaatga ctgaactgca ccccaaaagt tggacaaaaa aatctaacat
     6961 ttggttggac ttgtcaagaa aaaatagaca taaagttaag ctaatgagta actatttttt
     7021 caaattctac aggtgagaga tatcctaaag ctgaatgcag acgtttaggg ttatagtaag
     7081 tctcactata tttaaaaatc tcaagctgag cttgctcaat agttgcgaaa tgagcatcat
     7141 tgacaagctc tctttttaat gttttataaa aggcttccat gagtgcatta tcgtatggat
     7201 taccttttcg actcatactg gacttacatt ttttctgtct aagtaggtcc tgaaatcatg
     7261 ctccggtata ttgagaaccc tgatctgtat ggacgattac cccttcttta ggcttttctc
     7321 tattataagc ttgattgaaa gcctctgtta ccaacttgtc ttgcatacgt cggcccattg
     7381 cccaaccaac aatctttcta ctataaacgt ctatcaagac agacacataa agtattcctt
     7441 cttgtgtagg aatgtaggtt aagtctccta gccataattt attcttacca gtcgcttgga
     7501 aacattgatt aacaagattg ggacgagtt
//
LOCUS       DAUCWO010000070         7220 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUCWO010000070 DAUCWO010000000
VERSION     DAUCWO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.070"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            581..670
                     /locus_tag="VGY20_001496"
     CDS             581..670
                     /locus_tag="VGY20_001496"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4274328.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(708..1631)
                     /locus_tag="VGY20_001497"
     CDS             complement(708..1631)
                     /locus_tag="VGY20_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274329.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1624..2694)
                     /locus_tag="VGY20_001498"
     CDS             complement(1624..2694)
                     /locus_tag="VGY20_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274330.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQGLHQKILRKMSQQEEGNV"
     gene            complement(2703..3629)
                     /locus_tag="VGY20_001499"
     CDS             complement(2703..3629)
                     /locus_tag="VGY20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274331.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3629..5131)
                     /locus_tag="VGY20_001500"
     CDS             complement(3629..5131)
                     /locus_tag="VGY20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274332.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSFIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAVYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5196..7166)
                     /locus_tag="VGY20_001501"
     CDS             complement(5196..7166)
                     /locus_tag="VGY20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4274333.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYEKAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2248 a         1408 c         1339 g         2225 t
ORIGIN      
        1 tttttgtact tttttatttg cccgcgttgt aaaatgaagt gcaacaaaaa agagaaaaag
       61 ccttgaacta caccccaaac gttagacaaa aaaatctaac gttcggggta ttttttatga
      121 aattaagtga tgaaagtacg aaatagacat cgttaagaaa gaaaaaaata cctactatcc
      181 accagagata aagcaagaaa taatggataa cgttttgata gaaggcccgc cacaatacca
      241 agtatcactt gtttatggct tgttacatgc aggaactctc ccaagttgaa tagcacaata
      301 taagaaaaac agttatactg ttgagaaaac aagagcaaga ccatcacaaa caggacgtaa
      361 accaaaagaa acctgaagaa atgactaaat tagagcggct tcaagaagaa aatgaatatc
      421 tcagaactga ggttgcttac ttaaaaaaac cgaggaactc cgtttgaaag aagcatctct
      481 gcaaagaaga agacaaaaaa accattataa aaaatggaca aagacgagtt ctgattagat
      541 tttcttttaa gaacggctgt attgactcgt tctacctacc attatcatcg attattgcaa
      601 caataaacgt atcaaaataa aacgaaaagg acttagtcct ctacaataca aaactaaatc
      661 ctttacttaa aatagagtag ccaacttttg gggtcagtgc atctctttta caattctttt
      721 tgatattttt caatttctgc ttggtttgcc caaacaaagt gatttggttt gatttcaacc
      781 attgatggct tatctaatgt ataatcatgt tggtctggat gatagacaac aagttctttt
      841 tgacgctcta aaattggatc tgggataggc acggctgata acaaagattg ggtgtaggga
      901 tgaatcgggt tattaaacag ttcttctgtt tctgcaactt ctacaataac ccctttatgg
      961 ataaccgcaa tacgatctga aataaagcga acgactgaaa gatcatgggc aatgaagaga
     1021 taagtcaaac ctttttcggc ttgcatccgt ttgagaaggt ttaaaacctg tgcgcgaacg
     1081 gaaacgtcca aagctgaaat cggttcatca gcaataacga attcagggtt cattactagg
     1141 gctctagcga taccgatccg ctgacgttga cctcctgaaa attcatgagg gtagcgcgtc
     1201 aaatgctctg atagcaaacc aacttcggcc atcatgttct taattttttc tttacgttct
     1261 tcctctgttt taaacagatt aaagttataa agaccttctg aaatgatgta gtcaacggtt
     1321 gcccgttcat tcaaactagc agcgggatct tggaaaatca tttgaatttt acgaatgagc
     1381 tcattggctt ctgattttga ttttctgcca ttaattactt tcccatcgta taaaatttga
     1441 cctgaactag tatcgttcaa accaataata gcacgaccaa ttgttgtttt accactccca
     1501 gattctccaa ctaaagaaaa ggtttctcct tttttaataa agaaattagc atttttaact
     1561 gcaacaaatt ttttctttcc ttctccgaag gaaatttcta ggtcttttac ttcgactaat
     1621 ttctcagaca tttccttcct cctgttgtga cattttcctt aagatttttt gatgcaaacc
     1681 ttgaatgact tctggttttt gaacttttgg agcctctggg tgtaaaagcc atgttttggc
     1741 ccaatgagtc tcagatacgt taatcgccgg aggtgctttt tcaaagtcta aaaccatagc
     1801 atattctgag cgaagtgcaa aggcatctcc gataattggt gaataaagtg atggaggcgt
     1861 tcctggaata gcgtaaagtt caccagattc atctgccaac tgcggtaagc tagacagcaa
     1921 actccatgta taggggtgtc ttggatcata gaaaatctct tcgactgttc caaattcaac
     1981 aatttctcct gcatacatga cagccacttt atctgcaatg cttgccacaa cacctaaatc
     2041 gtgcgtaata aagataatgg tgaaatgata ttctcgttga agcgacttca ataactcaac
     2101 gatttgagct tgaatagtca catcaagggc tgttgttggc tcatcacaga taagaatatc
     2161 tgggcgacaa gctaaagcaa tagcgataac aatacgttga cgcattcctc ctgaatactc
     2221 aaatgggtaa tcttcaaagc gttttttggc atttgggata cccactttat tcatgtaatc
     2281 aagggccatt tctttagctt tggcatgact tactttttgg tgcttaataa tcacttctgt
     2341 gatttggcta ccgatagttt taatgggact aagactggtc attgggtctt ggaaaatcgt
     2401 tgcgattttt gagccgcgaa tctttgccca ctctttattt gtttttaaat ctgtcaattc
     2461 ttgcccacga tagacaattg aaccattagc aatgcgtcca ttagactcca acatccctgt
     2521 aaaggtcttt gttaaaacag atttacctga gcctgattcc cctacaaaag caaggacttc
     2581 tccttcaaca agttccagtg agacgttacg gatagctgtt aaaacacgat cacgcacatc
     2641 aaattctacc accacatctt tggcagttaa gattacatta ttttcttttg tcattttaac
     2701 tcctatctat gtgaacgtgg gtcactggca tcagccaagt tttgtccgac aatgtatagt
     2761 ggtaacgata ctaaaatcaa tgttactaag ggaatccaaa agaggtaggc atttgttgtt
     2821 aagttgcttg aataattagc aatcaaacgt cctaaacttg gagtggtggt tggtaaacca
     2881 atcccaaaga aggataagaa ggcctcagaa gatacataaa ctggtagcat ttgtgacaac
     2941 atagtcatga taactgaaac caattgaggc aggaggttct taacagcaat cttgtacatt
     3001 ggtgttccca aagtttgact agcaaggttg tattctaaat cacggtaacg caagatttga
     3061 acacggatgg agtaggcgac accaatccat ccagtgatac agaaagctag aatcaaattc
     3121 caaaatcctg cacctaatga ataggtcaaa acaatgataa taagcataga agggatattt
     3181 gagataatgt tataaatttc aatcataact ttatcaaatg ctttagaaac tccccatatg
     3241 gctcctaaca ctaccccaat ggtgatatta attagtgtcg ctataactga gattaaaata
     3301 gaattacgtg ccccatacca aacaccatca aacagagatt gtccattttt gtcggttcca
     3361 aaccagtact ctgcattcgg ccaaatataa cgctttgaaa agtcattgat attactaacg
     3421 tcattaaagt cgtagttggc aaacattgga taaataaagc tcatcatcaa gactgtcact
     3481 aaaatcacga gcataaagac tgtagatttt ttagaaaaaa actgacgaaa cactgatttc
     3541 cagtaagaat aagcaggggt atcaatcact tcggaggctt cactatcgcg ctcaacaaat
     3601 cgaaatttag atttatcaat cgattccatt acttacctcc tttttttgtt gacaatttaa
     3661 tacgtggatc gacaagggtc atgacaatat ctcctagtaa aagtgaaaca attgacaaga
     3721 cagtgaagat aaaggttaat ccaacaatca tagaattgtt agcagattta atagaatcaa
     3781 ttaacatttt acccataccc gggaaggcaa agacagtctc tgttaaggtt gcaccaccaa
     3841 tagcaaggat aatacttgca ggaacccctg acacaattgg gaccatggca tttttgaaga
     3901 gatgccctct gtaaatttca gattctgata atccttttga acgcgcaaaa cgtacccaat
     3961 cgctggcctg aaggtcaaca agatagcgac ggaaccagat aaccgtagtt ggaatattta
     4021 aaattcctaa aataagggct ggtaaaatat aagatttagg atcagatgcc cccagcattg
     4081 ggaaactatc tggtaaacca accatgccac ctaggaagcg aacaacatag ataacagcaa
     4141 tacttggtaa agcaagcatg aaggtcatgg tcgctgttga gaaacggtca aaataggtat
     4201 ttttaaaccg agccataaat aaaccaagag gcaaaccaac gatataggaa aacataacac
     4261 caaaaaaacc aattacaaaa gaattgtgaa tcattgatgg gtcgacataa ttattgatgg
     4321 ttttagtgta ggaatctccc ttaccatagt ttaccttggt gatatcatcc aatgattttg
     4381 gatttttgta gctacggcta tagatatcca ctgacgattt cttagtgaca cctgatggga
     4441 aggtcacatc ttgaagagct gttcgtcctt gcccttgtga aataacctga agaacaggaa
     4501 tattgctgta tgttggataa gatgttccta agtttagggt aacaaagttt tggtgaagat
     4561 aaggaaattt tccgttagta tagaggagat atttatgttt tgtccccgaa ccaacaagtg
     4621 accagccaac tgatttatct ttttctagac gaatatacct agctaatttt ggattatctt
     4681 tatcctgaat cttccaaggg tgatcaataa caactagatt tgagaaaaag ttccaaacac
     4741 gttcgtaaat aggaatattg cgaattgcat aaaactgttt gctagtgggg aagcgtttta
     4801 gctgccagcc attgccaaga gaatcaatat acttctcata gacagctttg ttagcactag
     4861 ttgcttctgt cgttacacta ctatctactt tagaagcatt atcttttaat tctttgcttg
     4921 aaaaataatt gacataaccc atacgttgaa aagttagatt ttcatacgct acttttttat
     4981 ctggtgttgt tgtcatttta ttataattag ggtcttgttt aaagataaag ctggtaggta
     5041 ctagagtata tacgatggtg tatgttaaag ccgttaccaa aactaccgac actaacgatc
     5101 gcatgatacg gtttaaaatg tattttttca tagttatcct caatagttca aaagaactcc
     5161 ctcacataaa cgagaaagtt cttttgaaca tcgttttatt tttcaacgtg atcagctaac
     5221 ttctcagcat attttgcatt tgacttagcc tttgctttca tccatttttc ttttgctttt
     5281 tcgtattgtt ttgctgtgac aggtttgtct tgaagtttca ttcctttata tgctagagga
     5341 cctttagacc ctgcccaaga gaagcccccg ctaaatggaa cggctttagt aactcgtgga
     5401 gtgccaccca atgccacaac tggaatatct acggcattat ctgtaaggta ggcttgtgct
     5461 tttgcgtaag ctttatagcg cgcatcgttg tcgtctgtaa ttgctgctgc ttcatcaaga
     5521 agagtttggt aggtatcaag gcctgcagct gccacaacat ccttattttg acctgctttg
     5581 attccaagtt tttggataac agatccacca cctactggac tcatgatgtc aaggtaggtc
     5641 cgtggatctt gatagtctgg tccccaccat gatgaaatga tatcgtagtc ttgttgttct
     5701 ggggtctcag catagaagcc ttgggcttcg tgagttgatg tttctgtttc aagaacattg
     5761 acaatgacat tttctttacc aagagatgct tcaacagatt gtttgaaaga ctgggcttcc
     5821 tgaacagttg ctgcgtttgc ttggtcaaca gggtaatcta attgaactgg gaaggttaca
     5881 ccttcagctg tcaaagcttc tttggctttt gcaaactcag cttttgcttt ttcaggatta
     5941 tagaaaccat cttgagcatc agctaagtta acgtctttcc attcatcacc aagttttgcc
     6001 atttcctttt caacttctga accaaaatca ctttctccaa tggtcacaaa tgttggtggc
     6061 acaagcatgt tacgtaaggc ttttgttttg gcatcttgac ctgcagtttg ggcttggaat
     6121 gacgctcggt caaaagcaaa ctgaatagct tgacgaaaat ccttgttgtt aagagctttc
     6181 ttaccggcat cttgttgtgc agggtctttc ttagtgtttt tgaaagaagt acggttcaaa
     6241 ttccatgtta aatgacggat atctccagtc aacattccgt aagtaatgtt atcagcatag
     6301 tttttcttag ctgatttgta ggtagggtca tttgggtaaa gtcgtgcaac gctgaactca
     6361 cccttgtcaa agttcttgta gaacgaacct gggtctgaac catctgagta agtcaattta
     6421 acagattcta tcccaacatt cttagcatcc cagtagtttt catttttatg gaattccata
     6481 gatgattttg aggtgaaggc gctcaagaag taagcaccat taacaaggat tgatgatgga
     6541 tcggttgtac caaaatcttt accttttgac ttcaaaaatt tcgcattaac tgggaaaagc
     6601 acactataag ttgtttttga attccagtag ctttcaggct tgttcaaagt atactgaaca
     6661 gttttatcgt caagggcttt gacaccaact tctttaaaat ctacttcacc attttggtaa
     6721 gcctttaagt tttttattga atcttcaaca acgtaaagag catctgattt atcgtcaacc
     6781 gcgtgcttca aaccagtcac aaaatcttct gctgttactg gggcatattc ttcaccatca
     6841 gccgtatacc aagagacacc atcgcgaaga gtataagtat aagtcagacc gtcttttgaa
     6901 accttccaat ctttagcaag tgatggtact aaattaccat actcatcgtt ttccaaaaga
     6961 ccatcaacca tttgtgttat cacatcagtc gttccaccgc cattagtcaa aatataatcc
     7021 aatgattttg gatcgttaac aaaaacgtac ttgtaggttt ttgtagcttc tgagccacct
     7081 gaagcatttt tattaccaca agctgccaaa gcggatactg acaagatcgc aacacttaca
     7141 gctgccaacc atttactttt cttcataaaa gtcaccccta aactttattt ttcatttttt
     7201 ggcaattgct aaatgaaatt
//
LOCUS       DAUCWO010000071         7118 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUCWO010000071 DAUCWO010000000
VERSION     DAUCWO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.071"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            180..1415
                     /gene="arcA"
                     /locus_tag="VGY20_001502"
     CDS             180..1415
                     /gene="arcA"
                     /locus_tag="VGY20_001502"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ4274334.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1515..1955
                     /locus_tag="VGY20_001503"
     CDS             1515..1955
                     /locus_tag="VGY20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4274335.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQTYGSKYLEDG
                     EYCQSLVKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNNEPKD"
     gene            1980..2993
                     /gene="argF"
                     /locus_tag="VGY20_001504"
     CDS             1980..2993
                     /gene="argF"
                     /locus_tag="VGY20_001504"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ4274336.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3163..4656
                     /locus_tag="VGY20_001505"
     CDS             3163..4656
                     /locus_tag="VGY20_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ4274337.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4673..6004
                     /locus_tag="VGY20_001506"
     CDS             4673..6004
                     /locus_tag="VGY20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ4274338.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGCDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            6024..6974
                     /gene="arcC"
                     /locus_tag="VGY20_001507"
     CDS             6024..6974
                     /gene="arcC"
                     /locus_tag="VGY20_001507"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ4274339.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALMSTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQTKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
BASE COUNT         2143 a         1351 c         1520 g         2104 t
ORIGIN      
        1 taaacgaata caaatctata aaaataaaaa caacgctcaa aaaatgtaag cgattactca
       61 aaatggctgt gagaaaaacc ctatttttat cttttttaag gagtacacta ggaatataag
      121 caagagttaa gaatcttgac ggtgaattca caaaaaccaa ttagaaagag gtaattctta
      181 tgactgctca aacaccaatt catgtttatt ctgaaattgg aaaattaaaa aaagtgttgc
      241 tccacagacc cggtaaagaa attgaaaatc ttatgccaga ctacttggaa cgcttgttgt
      301 ttgatgacat tccattctta gaggatgccc aaaaagaaca tgatgctttt gcccaagctt
      361 tgcgtgacga aggtattgaa gtcttgtacc ttgagacctt agctgcagaa tctcttgtca
      421 ctccagaaat tcgcgaagct ttcatcgatg aatacctaag tgaagctaat atccgtggaa
      481 gagctactaa aaaagccatt cgtgagctgt tgatggctat cgaagacaat caagaattga
      541 ttgaaaaaac catggcaggg gttcaaaaat ctgaacttcc agagatccca gcatctgaaa
      601 aaggcttgac tgacttggtt gaatccaatt acccatttgc gatcgaccca atgccaaacc
      661 tttatttcac acgggaccca ttcgcaacta tcggtacagg tgtttctctt aaccacatgt
      721 tctcagaaac ccgtaaccgt gaaacccttt atggtaaata cattttcact catcacccaa
      781 tttacggtgg tggcaaagtg cctatggttt atgaccgtaa tgaaaccact cgcattgaag
      841 gtggggacga acttgttctt tcaaaagatg tgcttgcggt tggtatttct caacgtacag
      901 atgctgcttc tattgaaaaa ttgttggtta acatctttaa acaaaacctt ggcttcaaga
      961 aagtattggc ctttgaattt gcaaataacc gtaaatttat gcacttagac actgtcttta
     1021 ccatggttga ctatgacaaa tttaccattc acccagaaat tgaaggcgac cttcgtgttt
     1081 actctgtcac ttacgacaat gaagaacttc atatcgttga agaaaaaggt gatttagcag
     1141 aacttcttgc tgctaacctt ggtgttgaaa aagttgacct tatccgttgt ggtggtgaca
     1201 acttagtagc agcaggtcgt gaacaatgga acgatggttc taacaccctt actatcgcac
     1261 caggtgtggt tgtggtttat aaccgtaaca ccattaccaa tgctattctt gaatctaaag
     1321 gcttgaaatt gatcaagatt cacggaagtg aattggttcg cggtcgtggt ggacctcgtt
     1381 gtatgtcaat gccatttgaa cgtgaagata tttaataagc tatggtaaag gtggttatag
     1441 gtcagaagcc tttttaaagg gcagctagtg tttatcttcg attctgtgtc tttgtccttc
     1501 aatgaaattt tgtcatgaca gataaatttg atgccaatga cgaaacaaga acggtttatg
     1561 cagtcgttta tgacaatgac cagcccgttt caacaggaca atttttagct gaaacgaaaa
     1621 tagaagcacg attgacacgc attgtaacct tagcagatta ttgtggttgc ggttatggtg
     1681 ccaaagtcac tgaagcgcta gaaacttata ccagacgaga aggcttttac caactaacca
     1741 ttcacagtga actgacagca caaacctttt atgaaaacct aggttatcag acctatggtt
     1801 ccaagtattt agaagatggt gagtattgtc aatcccttgt taaaaccatt cttaaatggg
     1861 agaagaatat ggacatagca atgctaattg cgattgttgg tggtctatta ggctgctatc
     1921 tctatctcac aaaaaataat gaacccaaag attaagttaa tactcgaagg agacaataga
     1981 tgacacaagt atttcaagga cgtagcttcc tagcagaaaa agattttaca cgcgctgaat
     2041 tagaatacct tattgatttt tcagctcatt tgaaagattt gaaaaaacgt ggtgtgcctc
     2101 atcactactt agaaggtaaa aacattgccc tcttgtttga aaaaacatca actcgtacgc
     2161 gtgcagcttt tacaacagca gccattgacc taggtgctca cccagaatac cttggtgcca
     2221 atgacatcca acttggtaaa aaagaatcaa ctgaagacac tgccaaagta ttgggtcgta
     2281 tgtttgatgg gattgaattc cgtggcttta gccaacgcat ggttgaagaa ttggcagaat
     2341 tctcaggtgt gccagtttgg aatggtttga ctgatgaatg gcacccaacc caaatgcttg
     2401 ctgactattt aacagtgaaa gaaaactttg gcaaacttga aggccttacc cttgtttact
     2461 gtggtgacgg gcgtaataac gttgccaatt cccttcttgt gactggtgct atccttggtg
     2521 ttaatgttca tatcttctca ccaaaagaac ttttcccaga agaagagatt gtgacattgg
     2581 ctgaaggcta tgctaaagaa agtggcgctc gtatccttat cacagaagat gctgacgaag
     2641 ctgttaaagg cgcagacgtg ctttacacag atgtttgggt atcaatgggt gaagaagaca
     2701 aattcaaaga acgtgttgaa ttgcttcaac catatcaagt caacatggac cttgttcaaa
     2761 aagcaggaaa tgacaagctt atcttcttgc actgcttgcc agctttccat gacacaaata
     2821 ccgtttatgg taaagacgtt gctgaaaaat tcggcgtgaa agaaatggaa gtcactgatg
     2881 aagtcttccg cagcaaatac gctcgtcatt ttgaccaagc tgaaaaccgc atgcacacta
     2941 tcaaagcggt tatggcagca acccttggta acctctttat tccaaaagtg taataaggac
     3001 tggtactcct taactcattt cacatatcaa tgtgacccct ttaatttaac aatttaacta
     3061 cttaaaaacg tactttaact acatgacaag tcgttctggt tttcgcgtcc ttgaccggaa
     3121 ctagaacggc ttatttttat gaaaaggtaa gaggtaatca ttatgacaga agaaaaaaaa
     3181 cggggtttta gaattccttc ttcttacacc gttcttttta tcattatagc catcatggca
     3241 gtgttaactt ggtttattcc agctggcgcc tacgaaaccg ctaagggcgg tggtgttatt
     3301 tcaggaacct acaaaacagt agcatccaat cctcaaggat tttttgacat cttgatggct
     3361 cccgttcgag gcatgttagg tgttgaaggc actgatggtg ctattcaagt gtctttcttt
     3421 atcttaatgg ttgggggctt tttaggagtt gtcaacaaaa caggtgctct tgacacaggg
     3481 attgcgtcag tggttcgtaa aaacaaaggt agagaaaaaa tgctgattgc tattttaatc
     3541 cctttgtttg ctctaggtgg aacgacctat ggtatggggg aagaaaccat ggccttttac
     3601 ccacttttaa ttcctgttat gattgcggtt ggttttgaca gtattgttgc tgtagccatt
     3661 attttgattg gttctcaaat tgggtgtcta gcctccacta ttaacccatt tgcaactggt
     3721 gtagcagctg atgctgctgg tgtcagcatt gcagatggaa tgatttggcg tgttatccaa
     3781 tgggttattc ttgtcggcat gtctatttgg tttgtttaca attacgctag taagattgaa
     3841 gaagacccaa gtaaatcatt ggttgcagac aaggaagaag agcataagga actcttccag
     3901 ttgcaaaatt ctggagaaga cttaaacaag cgccaacgaa acgttttgac catttttact
     3961 ttgacctttg tcattatgat tcttagtttg attccgtggg aagattttgg catcaaattc
     4021 tttactaata tcaatacttg gttaaccaca atgcctatct tagggggagt tattgggaaa
     4081 accatgggag catttggtac ttggtatttc ccagaaatca ccatgctctt tatcatgatg
     4141 ggtgtcttag ttgctattgt gtatcgtatg agtgaagaag acttttttag ctcttttttg
     4201 actggtgcag gtgaattttt aggtgttgcc atgatctgtg ctattgcacg cggtattcaa
     4261 gttatcatga atggtggtat gattacagcc actatcttac acttaggtga aacaagtctt
     4321 tctggtttat cttctcaagt ttttgtgata ttagcttata ttttctacct cccaatgtcc
     4381 ttcttgattc catcaacatc aggacttgct ggggctacaa tgggaattat ggcaccgctt
     4441 ggacaattct caaatgtccc tgctcacctt gttattacag ccttccagtc agcttctgga
     4501 atcttaaaca tgatttctcc aacttcagca atcgttatgg gagcacttgc gcttggtcgc
     4561 gttgaccttg gtacttggtg gaaattcatt ggtaaattta ttgtaatggt gatgcttgta
     4621 agcgtgctat tacttgtagt tgcaacattc ttttaacaga taggaggttc ctatggaatc
     4681 ctatatcaca cctaaacatc aagaggcctg tgtagctgcc attaaacaga tcgtttctta
     4741 cccttctgtt tgtcatgaag gagaaaatgg cacacctttt ggacaggcca ttcaagatgt
     4801 tctcgaagca accttagact tatgtcaagg tcttggtttt catacttata ttgatcctga
     4861 aggttactat ggctatgctg agttaggtga ccaaaaagag gtcttggcta ttctttgcca
     4921 tttggacgtt gttccagaag gagatcgtca gttatggaag acagatcctt ttgactgtgt
     4981 tgaggcagac ggctgtttat ttggtcgagg cactcaagac gataaagggc ctactatgat
     5041 ggcgctattt gccaccaaag ccttgataga tgcaggcgtt acctttaata aacgcattcg
     5101 ttttattttc ggaacagatg aagaaacttt gtggcggtgt atgaatcgct acaatgatgt
     5161 agaagaacag gctacctttg ggtttgctcc tgattcaagt ttccctttaa cctatgccga
     5221 aaaaggccta cttcaggcaa aattagtggg gaaaggttgt gataaactgt cacttgaaat
     5281 cggacaagcc tacaatgttg ttcctgcaag agcttcttat aaaggcaata agcttgaggc
     5341 tttacaaaaa gaactagata agcttggctt tgagtacgtc gtcaaagagg gagaattaac
     5401 ggtttatggt ctcgcccaac atgctaaaga tgctcccgat ggcatcaacg ctatgattag
     5461 gcttgccaag gctttagtgg tccttgaacc taacaaaacc cttgattttc ttgctaatgt
     5521 cattgatgaa gatggcagag cacttaatat ttttggcaga atcgaagatg aaccttcagg
     5581 caaattaagc tttaatgccg cagggcttac cttaaccaaa gacaaggcag aaattcgcct
     5641 agatattcgg attccagttt tggcagataa ggaaaaatta gtccaacaat taagccaaaa
     5701 agcacaagaa tacggcctaa cctatgaaga atttgattat ttggccccat tatatgtgcc
     5761 attagacagt gaattggtaa caaccttgtt atctgtctat cggaaaaaaa caggtgatca
     5821 aagccctgcc caatcatcag gaggggcaac ctttgctaga acaatgaaga attgtgttgc
     5881 ctttggtgcc cttttccctg atgctgttca aacggaacac caagaaaatg aacatattgt
     5941 tttagcggat gcttaccgcg ctatggatat ttatgcggaa gctatttacc gactaacgcg
     6001 ataacaagat atgaggagta attatgacga aacaaaaaat cgtagtcgca cttggaggaa
     6061 atgctattct ttcaacagat gcgtcagcaa aagcacaaca agaagccttg atgtccacat
     6121 caaaatcatt ggttaaatta attaaagaag gtcatgaagt catcgtgacc cacggcaatg
     6181 gcccacaagt tggtaatctc ttattacaac aagcagcagc agattctgaa aagaatccgg
     6241 ctatgccttt ggatacttgt gttgccatga cagaaggtag catcggtttt tggttagtta
     6301 atgcccttga taatgaattg caagcccaag gtattcaaaa agaagtggca gctgttgtca
     6361 ctcaagtgat cgtggatgcc aaagacccag cttttgaaaa cccaacaaaa ccaattggtc
     6421 catttcttac cgaagaagat gccaaaaaac aaatggcgga gtctggtgca agtttcaaag
     6481 aagatgcagg tcgtggctgg cgtaaggttg tgccatcacc aaaaccagtt ggtattaaag
     6541 aagccaacgt catcagaagt ctggttgatt ctggcgttgt tgtggttagt gctggtggtg
     6601 gcggtgtgcc cgttgtggaa gatgcaacta gcaagaccct tacgggtgtg gaagccgtta
     6661 ttgacaagga ctttgctagc caaacccttt cagaattggt ggatgcagac ctctttatcg
     6721 ttttgacagg ggttgacaat gtttatgtta acttcaacaa acctgatcaa acaaaattgg
     6781 aagaagtaac ggtctctcaa atgaaagagt acatcacaca agaccaattt gcaccaggta
     6841 gtatgttacc aaaagtggaa gctgccattg cttttgttga aaacaaacca aatgctaaag
     6901 ccattatcac atcgctcgaa aatattgaca atgtactatc agcaaacgct ggaacacaaa
     6961 ttatcgcagg gtaatcaaga ggatttttag ttctgtttaa aagactcatt taatggtatt
     7021 ttctgaagag tacgactgac ataaagagtt tatactgttt ttaaaggtaa agagtgtgcc
     7081 ataataaaaa acaataatag tattagaaaa ggctaatt
//
LOCUS       DAUCWO010000072         6723 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUCWO010000072 DAUCWO010000000
VERSION     DAUCWO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.072"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            43..381
                     /locus_tag="VGY20_001508"
     CDS             43..381
                     /locus_tag="VGY20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274340.1"
                     /translation="MKKRFNSKAITLLAVSSLSLGFTAVTSVVSAATAHPAPSPIIDE
                     WNYGVSDNYGYSNYYVKSPNNIGSKSSITNYWGSIKASDSQKYGWALSSSKKAWNDIR
                     LNAHWNYFNF"
     gene            449..2431
                     /locus_tag="VGY20_001509"
     CDS             449..2431
                     /locus_tag="VGY20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274341.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKSAPITGMYYTL
                     GKIDSSSLKVLTASGLKLVYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQISQLSPWNKVRNWKRLELLGIENGSINNGEINEPESQYIQI
                     ATALKKLDFIYIKPSSVYIPDYMKNTNFAEDFFKKLNNDGITDPEVNKEMIYINKSGA
                     NIQNKVNGTDYQLLDNKVATIYIPDKFKEKKKSIENTVVAEQFTGTKYTKENLAVQII
                     PNEKKIFYFNENGNEHDENKEASPMANVTDSKGNIVVVLDTDKMVASKDFSLASNIVN
                     SSLFSPEAIKKINDLSLHLNFSINPVDVYQLVKLNIQSLKHQIFLSKVLQKIIYGIVF
                     LLIYQYAQLFISSKQNDYVKKIILGLSKTRIAISSLKYFIMTIAIVIICTFMVTEQIE
                     LLYIGIASLVVLILSTVMSFRKLSKKYTQILKGDEQ"
     gene            2428..3051
                     /locus_tag="VGY20_001510"
     CDS             2428..3051
                     /locus_tag="VGY20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4274342.1"
                     /translation="MTIEIFNVTKKFSSKKIFTDLNLTFEAGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDNGKVLVDEQDIWKIKERTFFKNIIGYVFQNYSLIENKTVYDNLKLIN
                     KDKKKISAVLEKVGLSSDYLNQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIINLLLSETAENKYVIIATHDPAVYNKVDFIIDMKDIGSKK"
     gene            3048..3539
                     /locus_tag="VGY20_001511"
     CDS             3048..3539
                     /locus_tag="VGY20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274343.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPEVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            3743..4273
                     /locus_tag="VGY20_001512"
     CDS             3743..4273
                     /locus_tag="VGY20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ4274344.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            4322..4909
                     /locus_tag="VGY20_001513"
     CDS             4322..4909
                     /locus_tag="VGY20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ4274345.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            5062..5799
                     /locus_tag="VGY20_001514"
     CDS             5062..5799
                     /locus_tag="VGY20_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ4274346.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SSYLQKFWLVYVAMGLFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRR"
     gene            complement(5856..6155)
                     /locus_tag="VGY20_001515"
     CDS             complement(5856..6155)
                     /locus_tag="VGY20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ4274347.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDETTKKTLGAFAGKLPF"
     gene            complement(6348..6722)
                     /locus_tag="VGY20_001516"
     CDS             complement(6348..6722)
                     /locus_tag="VGY20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274348.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLENKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
BASE COUNT         2344 a         1021 c         1247 g         2111 t
ORIGIN      
        1 taatttttta aaaattaaat aattttatta aaggagaata gtatgaaaaa aagatttaat
       61 agcaaagcga taactttgct tgctgtaagc tctttaagtt tgggatttac ggccgtaact
      121 agtgttgtca gtgcggcaac agcacatcca gctccatctc caattattga tgaatggaat
      181 tatggagtaa gtgacaacta tggttattct aattattatg ttaaatctcc aaacaatatt
      241 ggttcaaaat cttcgataac taattattgg ggatccatta aggcatctga ttctcaaaaa
      301 tacgggtggg cactgtcaag ttcaaaaaaa gcttggaatg atattcggtt gaatgcacat
      361 tggaattact ttaattttta gaaatggtat atgcaagcta aaggcccaat aattattata
      421 gggtctttta taatatagga agttgtttat gaaattaaaa ttatctgtca ttggattatt
      481 cttttgctta attgctgcga taggtgttat cagtattagt gatactcaaa aaccggtacc
      541 tttaccaatt gatggtgcct tttcaatcca aggaaaaagt agcctttcta gtgacgaagt
      601 ttacaaaaag attagtaatc tatcaaaaac gaaaaacgtt aatatttaca agcctatagt
      661 tcgtaactca ggtcagttga cttatatcaa ttttaaaaat acaaattatg aacaattaaa
      721 atctgcaccg ataacaggca tgtattatac cttaggtaaa atagattcaa gtagcttaaa
      781 ggtacttaca gcttcaggtt tgaaacttgt ttatgcagcg tatccttggt atataggggg
      841 gattttacaa tttgatggga ctttaaggtt gttattgatg ctatcaattt acttaacatt
      901 attagttgtt ttatttgttg ttagaacgag gcagattaaa gaaggagtga taagacattc
      961 tttaggattg cctatatata acttaagaag agattatgtc atttctttta cttttgaatt
     1021 ggttatggct ggtttattaa tgattgccta cagctatttt tggggaagtg gcctctttac
     1081 ttacagttct aagttatttt tctctctaat tttaactaat cttataatat tccagattgt
     1141 tgacattatc acttttattt tattttggct aactattagg gttgagaaac ctattgaaat
     1201 tataaaaaat aaagcaaaaa atagttttct ttttattgta tggcttgtaa ttatttctat
     1261 tataataatc gtctcaggaa tctttttaca agaaacaaag gctagtcaat caaggataaa
     1321 tagacagata agtcaactat ccccatggaa caaagtaaga aactggaaaa gactagagtt
     1381 gttggggatt gaaaatggct ctatcaataa tggggaaata aatgagccag aatcacaata
     1441 tatacagata gcgacagcct taaaaaaatt agattttata tatatcaaac cctcttcagt
     1501 atatattcca gattacatga aaaatactaa ctttgcagaa gattttttca aaaaattaaa
     1561 taatgatggt ataacggatc cagaagtaaa taaagagatg atttatatca ataaatcggg
     1621 cgctaatatt caaaataaag tcaatggaac agactatcag ctgttagata ataaggtagc
     1681 aacaatctat attcctgata aatttaaaga gaaaaagaaa tcaattgaaa ataccgttgt
     1741 ggcagagcaa tttactggaa ctaagtatac aaaagaaaac cttgcagtgc agataattcc
     1801 taatgagaaa aaaatttttt attttaatga gaatggaaac gagcatgatg aaaataaaga
     1861 agcctcacct atggcaaatg taacagatag taaaggtaat atagtcgttg tgttagatac
     1921 tgataaaatg gtagcaagca aagatttttc tttggctagc aatatagtaa atagctctct
     1981 ttttagtcct gaagcaataa aaaaaattaa tgacctgagt ctacatttaa atttttcaat
     2041 taatccagta gatgtttacc aacttgtaaa acttaatatt caatccttaa agcaccaaat
     2101 tttcctttcg aaagttttgc agaaaattat ttacggcatt gtatttctac taatttatca
     2161 atatgctcaa ttatttattt cttcaaaaca gaatgactat gtgaaaaaaa taattttagg
     2221 cctctcaaaa acacgcatag caatttcgag tctcaaatat tttattatga ctattgcaat
     2281 agttataata tgtacattta tggtgacaga gcagattgag ttattatata ttggtattgc
     2341 ttcattagtg gttttaattc tttcgacagt aatgagtttt agaaaattat caaaaaaata
     2401 cactcaaatt ttaaaaggag atgaacaatg acaattgaaa ttttcaacgt tacaaaaaaa
     2461 ttcagctcaa aaaaaatatt tacagattta aatttaacgt ttgaagctgg aaaaagctat
     2521 gccttgattg gaggttctgg ctctggtaaa agtactctgc ttaacattat aggaagacta
     2581 gaaaaaattg ataacgggaa agttttagtt gatgaacaag atatttggaa aataaaagaa
     2641 agaactttct ttaaaaatat tatcgggtat gtattccaga attattcttt aatagaaaac
     2701 aaaactgtat atgataattt gaaactcatc aataaagaca aaaaaaaaat atctgctgta
     2761 cttgaaaaag tagggctctc tagtgactat ttaaatcaaa aaatatatga attgtctgga
     2821 gggcaggctc agcgtgtagc tattgcaaga atgttaatga agccacgtaa aataatttta
     2881 gcagatgagc caacaggagc tttggatggt gaaattggaa aagaaattat taatttgttg
     2941 ttgagcgaaa cagccgaaaa taaatacgtt attatagcaa ctcatgatcc agctgtatat
     3001 aataaggttg attttataat tgatatgaaa gatataggga gtaagaaatg aaaaagaaaa
     3061 tttatattgg cttgattgtc attgttggag tttttgtagt gttcttgtct ggaaaacaaa
     3121 tcacacaaaa agaaacttca aataagcctg aggtgaccct aacagtttat accatctcct
     3181 ctagtgatac ccaaaaatgg agtaaagtaa agcagtttga aacgaaagaa gcaatatacc
     3241 tagtaaaggt aaaagaaact acctcttcag aagaagtctt ttcaaacatc attgcagatg
     3301 gaatggcagt tggttttgga gttagtgaag aagacgttaa acagtttaac gataatttat
     3361 gtagcccggt agaagatgct aagaataata aattaattgg aatagaattc atcaattttt
     3421 ctagtgttga taaggggttt gtggttgcaa attttgacta cggcaaaaaa gcgtttaatt
     3481 ctcagaaaac agaaaataaa aaattatata aaaagctcta tgaagcgtat aaaggatagc
     3541 tatttatagt ttcttgagag tgataccact tctgaataat aaatgaacta acttggcata
     3601 ccgttttcta ttcatctaac aatgttttca agaaataatt gatgccttta gcacggctga
     3661 aatgaggtca aattgacaga atactcctat tatggtacta tttttaataa gaatattcag
     3721 tgatagtgat aaagaggaaa atatgggaat tgaaaagaca gttagtgaac tagctgatat
     3781 tttgggagtg agtcgtcagg ccgtcaataa tcgtgttaag tctttacccg aagaagatct
     3841 tgacaaaaat gaaaaaggcg ttactgtggt taagcgcagt ggtcttgtta agctggaaga
     3901 gatctataaa aaaacaattt ttgacgatga gcctatcagt gaagaaacga aacaacgtga
     3961 gcttttagag attcttgtgg atgagaagaa cactgaaatc acgcgccttt atgagcaact
     4021 caaagccaaa gatgctcaac ttgcctcaaa agatgagcaa atgcgtgtta aagacgtgca
     4081 gatcgctgaa aaagataaac aattggatca gcagcaacaa ttaactgcta aagccatggc
     4141 tgataaagaa accctaaaac tagaattgga agaagctaaa gcagaagcca accaagctcg
     4201 cttacaagtc gaagaagttc aggctgaagt tggccctaaa aaaggctttt taacccgctt
     4261 gtttgcgaaa taatcacaaa aaatgacgaa ggccctatca gggtcttttt tggaggaaaa
     4321 catgaaacac ttagatactt atattgcctt tgatttagaa tttaacactg tcaatgacgt
     4381 tagtcatgtc attcaagtat cagcagtcaa atatgaccac cataaagaag ttgacagctt
     4441 tgacacctat gtttacacag atgttccctt acagagcttt atcaatggcc taacaggaat
     4501 cacatctgac aagattgctg cggcaccaaa agtagaggag gttatggctg cgtttaaaaa
     4561 ttttgtggga gagttaccac tgattggtta taatgctcaa aaatctgacc tgcctattct
     4621 agctgaaaat ggcttagatt taagggacca ataccagatt gacctctttg atgaagctta
     4681 cgatagacgt agcgcagact taaatgggat tgctaacctt cgtctgcaaa cagtggcaac
     4741 ctttttaggc attaagggtc gaggtcataa tagcttagaa gatgccagaa tgacagcagc
     4801 aatctataag tcttttttag aaacagatac aaacaaagca tatctcagcc agcaagaaga
     4861 ggtaaccaca gataaccctt tcgcagcttt gggagacttt tttgactaag ggctctgcta
     4921 tttctaaaaa ataacaaaaa ttcctagggt tagctcttgc ttgtaacgct aggtcatgtg
     4981 ataagatagg ggcatatcaa gagggagtcg agtgcacacg aatcgttttt gatcactaaa
     5041 tgatagaaag aaggtgtgga aatgtctcaa ttctattcga ctggagaatt ggccaactta
     5101 gctggcgttt ctattcgaac tgtccaatac tatgatcaac gtgggatttt gattccgaca
     5161 gctttgacag aaggtggacg gcgactctat acggattctg atttagaaca attacgcatg
     5221 atttgctttc tgcgcgattt gggattttcc attgagcaga ttcgcaaagt cttagcagag
     5281 gaaaatgcag cccaggtgct ggaattgctt ctagttgatc atattgcgac tgccaaggaa
     5341 gacttggccg caaaagagca acaagttgac atagctgtca agatacttga cagacttcga
     5401 aaacaagacc cccagtcact agattttctg atggacattt cgcttagcat gaaaaatcaa
     5461 aaagcttgga aaaaattaca atggaagatg ggtatcagta tatttttagc cattgtttca
     5521 tatgtaggac ttattttact atctagttat ctgcaaaaat tttggttagt ttatgttgct
     5581 atgggactat ttctaccagg tttcagttgg ttggtaattt attttaggaa gcaatttatt
     5641 tatctctgtc cccattgtca tcagaaattt gaacctagct ttaatgaatt tgcccttgca
     5701 ggccatacac cacgaaccag aaagttaacc tgctcccatt gccatcaaaa atcgtattgc
     5761 ttggaactgg caaaagagga cactgtctca agaagataag cactaactca taaaaaaact
     5821 agcctgagat ccttctcaag ctagtttttg tttttttaga atggtaattt accagcaaaa
     5881 gcgcctaatg ttttcttagt tgtttcatca atttgcgtaa gggcatcatt aatggcttga
     5941 gtggtcatgt cttgaagcgt ttctacatct tcaggatcta caacagcttc tttgaagtca
     6001 atcccaacca attttttatc acccgtgaaa gtggcagtga caaggtcttg agctgatttt
     6061 ccagtaaatt gcatagcagc caaatctgct tgtttttgtt ccatttgttt ctgaagcttt
     6121 tgtgcctgct tcatcatatt ctgcatattc atcataagac tatactctcc ttaatttaat
     6181 actaactatt ataacatata aaaagaggca ataaaaacaa tgtcagttag tagactagcc
     6241 tgtatcagta agaaagtcgc tatggataag atggattagc agcaagggtc tggctaatga
     6301 cagaataagt ctagaaatga actatttttg tcatagacta gtccgactta gtggttatct
     6361 aattcacgat cttctttagc ttgttgttct aaaagaattt gtgcgaagtc aacccactta
     6421 gtgtagttat tttcggataa ttgttctgaa atcatcgcta actctcgttg tggcaactga
     6481 taagagactc ccttattttc taaggttaaa tcttcttcca ttagggcggt taaactaaca
     6541 tcaaagatag cagcaatatc acttaataga ccagactttg gagtatattt tcctttttcc
     6601 cactcactga ttgaagaaga gctttttcgt cctaggagat tagcaagttc taattgttcc
     6661 atgttctttt ttaatctaag gtactttaaa ttacttgcaa aaaaagtttt cttggcggac
     6721 ata
//
LOCUS       DAUCWO010000073         6644 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUCWO010000073 DAUCWO010000000
VERSION     DAUCWO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.073"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1244
                     /locus_tag="VGY20_001517"
     CDS             <1..1244
                     /locus_tag="VGY20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ4274349.1"
                     /translation="YYVKLENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESR
                     DAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLE
                     LLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAG
                     SKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRV
                     TVDPLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWT
                     SKIYHTLYEDGKIDDSFDVDVEIHHNFQFVLISLDTPEPIAMSNYIRQKLATIKISKE
                     FTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDV
                     VTIGKAFFEKADASDFTVFPK"
     gene            1294..2319
                     /locus_tag="VGY20_001518"
     CDS             1294..2319
                     /locus_tag="VGY20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274350.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            2333..2875
                     /gene="pgsA"
                     /locus_tag="VGY20_001519"
     CDS             2333..2875
                     /gene="pgsA"
                     /locus_tag="VGY20_001519"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ4274351.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            2877..3716
                     /locus_tag="VGY20_001520"
     CDS             2877..3716
                     /locus_tag="VGY20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ4274352.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            3692..4534
                     /locus_tag="VGY20_001521"
     CDS             3692..4534
                     /locus_tag="VGY20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ4274353.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            4527..5327
                     /locus_tag="VGY20_001522"
     CDS             4527..5327
                     /locus_tag="VGY20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ4274354.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            5699..6313
                     /locus_tag="VGY20_001523"
     CDS             5699..6313
                     /locus_tag="VGY20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEQ4274355.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKVPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     ATNASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT         2190 a         1055 c         1243 g         2156 t
ORIGIN      
        1 tttactatgt aaaattagaa aatggtttaa ctgtttattt tataaaaaaa atagggtttt
       61 tagaaaaaac agctatgttg acagtaggtt tcggttctct tgacaataag ttgacagtcg
      121 atgacgaatc acgagatgct ccagcaggta ttgcacactt tttagaacat aaactctttg
      181 aagatgaatc aggtggagat atttcattaa agtttacaca gctaggagca gagacaaatg
      241 cctttacaac tttcaaccaa acgagtttct tcttttcaac agctagtaaa tttcaggaaa
      301 atttggaact cctccaatat tttgttcttt ctgctaatat aacagatgaa tctgttagta
      361 gagaaaaaaa aataatcggg caagaaattg atatgtatca agatgatgct gactaccgtg
      421 catatagtgg aatccttcag aacttatttc caaaaacaag tttagctaat gatattgctg
      481 gctcaaaagc atccattcaa aagatcacga aaatattatt ggaaacgcat catacttatt
      541 tttaccaacc tactaatatg agtctattca ttgtcggtga tattgatatt gacgaaactt
      601 ttttagctat tcaaaggttt caaaccacat tatcctatcc agatagaaaa agggtaactg
      661 ttgatccttt acactactat cctgtaatta aatcatcttc tgttgacatg gatgttacta
      721 ctgccaagct ggttgttggt tttcgagggt atctgacctt aactcagcat tcactattaa
      781 cgtatcgtat tgccttgaag ctatttttat caatgcttat aggttggact tctaaaatct
      841 atcatacgct atatgaagat ggtaaaatag acgattcatt tgatgtggat gttgaaattc
      901 atcataattt tcaatttgta ttaatatcac ttgatacacc agagcctatc gcaatgtcta
      961 actatatccg tcaaaaatta gccactataa agatatcaaa agagtttaca aatgagcact
     1021 taaatctatt aaaaaaagaa atgtatggtg actttattca gagtttagac tctatagaac
     1081 atttaactca tcagttcagt ctatacctgt ctgattctga taaagaaaca tattttgata
     1141 ttccaaaaat tatagaaaga ctcactctaa aagatgttgt tacaattgga aaagcatttt
     1201 ttgaaaaagc tgatgcttca gacttcacag tcttcccaaa atagatgaaa atctgttata
     1261 atagggttac tatattattt ttgaggtacc tgtatgagtg aaaaaagtct tggtgaagtg
     1321 ttaagagaat cgcgtgttgg aaaaaatatc acactagatg atatagaatt gaagacaggg
     1381 atttcttccc attacttgct ggcaatggaa ttggatcagt ttaaaattat tccggaagaa
     1441 aaatttgatc aatttttaaa agaatatgct gacattgttg agttagattt caatacttta
     1501 aaaagacgat accgttatca agttaattcc aaaaaagaca gtgatataaa atctgtaact
     1561 gagattgttg aagagaagtt aagtaagaaa cgattacaag agaagttgtt atcacatcag
     1621 agcatggggt atggtcaacc gttagaagtt tctaaaaaca aagaaacctt agttatccct
     1681 gtaaagactg ttggtttaga tagcaaatcg ccactaaaaa cgatatcgcc tgttagccaa
     1741 tttaaatcag ataacaggtt aattcctaaa aaaaatgtgt cacgtatgag gcgaaatagc
     1801 actgacagtc gttctaaaac gtcactattt cccatagtta tattaggaac gattgctttt
     1861 gcaattatca gtgttatcat tttaggtgtt tggcaacaat tcgaaaagag tcaaagagct
     1921 aaagaagctg aagttgcttt gcttaaagcc tctgagcagt ctactaacgg tttaaaaaat
     1981 aattcaaatg atcgcaaaac tcaagtcaca actgaggggt cgggtaatta tttaatagcg
     2041 actgttacta aatctaaaga gaccgttgat atcagtgtgt ctttaaccga ggcacaaagt
     2101 agttggattt ctctaacaaa ttcagaaatt ggtgaaggtg gtataacatt gactcaagac
     2161 tcgccaactt atacagcgac tttacctgca gaaataacag aatcattgtt gacacttggt
     2221 gtgacaaaag gtgtatctgt aacaattgat ggacaacctg ttgatctatc acctttaaca
     2281 agtactgact taagttatat cacgtttaag atccaataat taaggtagac taatgataaa
     2341 aaaagaaaat attcctaatc tattaactct tgttcgaatc gcaatgattc ctttctttct
     2401 ttttattact tcctcatcta ataaggtggg gtggcatatt tttgcagctg tgatttttgc
     2461 aattgccagt tttaccgatt atctagatgg gtatcttgcg cgtaagtggc atgtagccag
     2521 taattttgga aagtttgccg atcctctagc agataagatg cttgtcatga gtgcctttat
     2581 catgttagtt ggacttggct tagttcctgc gtgggtgtca gctgttatta tttgccgaga
     2641 attggcagta actggtcttc gattactact tgttgaaacc ggaggaaagg tccttgcagc
     2701 tgctatgccg ggaaaaatca aaacagcgac acaaatgttg tctattattt tattactttg
     2761 ccattggata ttcctaggaa acgtcttact ctatattgct ctttttttca ctatttattc
     2821 tggatatgat tattttaaag gagcaagctt tctttttaag gatacgttta aataacatgt
     2881 cagctattat tgaacttaaa aaagttacat ttaattacca taaagaccaa gaaaaaccaa
     2941 cattagatgg tgtatcgttt catgtgaaac aaggtgagtg gctgtctatc attggccata
     3001 acggatctgg taaatcaacg actattcggt taattgatgg attattggaa ccagaatcag
     3061 ggtctattat tgttgatgga gatctactaa ccataactaa tgtttgggaa attcgtcata
     3121 aaattggtat ggtctttcaa aaccctgata atcagtttgt tggagcaact gttgaagatg
     3181 atgttgcttt tgggcttgaa aataaaggta ttgcacatga agatataaaa gagagggtta
     3241 atcatgcttt agagttagtc ggcatgcaga actttaaaga aaaagaacca gcccgtttat
     3301 ctggtggcca aaaacagcgc gtagctattg caggtgcagt tgctatgaag cctaaaatta
     3361 ttattttaga tgaagctact agtatgcttg accctaaagg acgattagag ttaatcaaaa
     3421 ctataaaaaa catccgtgac gactaccagc tgactgttat ttccattact catgacttag
     3481 atgaagttgc tcttagtgat agagttttag tgatgaaaga tggtcaagtg gaatcaacat
     3541 caacaccaga acaattattt gcaagagggg atgaactact ccaattaggt cttgatatcc
     3601 cttttacaac atctgttgta cagatgcttc aagaagaagg ttatcctgtt gactatggat
     3661 atctcacaga aaaggaatta gaaaatcagt tatgtcaatt aatctccaaa atgtaagtta
     3721 tacttatcaa gcagggactc cttttgaagg gcgtgccctt tttaacatta atttagatat
     3781 tttagatggt tcttatacgg cttttatcgg acatactgga tccgggaaat cgaccattat
     3841 gcaattgcta aatggtttac acgttccgac aacgggaatt gtcagcgtag ataaacaaga
     3901 tataacgaat cactctaaaa ataaagagat taaatctatt cgaaaacatg taggattagt
     3961 ttttcaattt ccagaaagtc aactatttga agagacagtt ttgaaagatg ttgcttttgg
     4021 ccctcaaaat tttggggtct ctccggagga agcagaagct ttagcgcgtg aaaaactagc
     4081 tcttgttggg atttcagaaa atctttttga aaagaatcca tttgaactct cagggggtca
     4141 aatgcgtcgt gttgctattg cgggtatcct tgctatgcag ccaaaggttt tagttttaga
     4201 tgaaccgact gctggtcttg atcctaaagg gcgtaaagaa ttaatgacta tttttaaaaa
     4261 gttacaccaa tctggcatga ctattgtttt agtaacccat ctgatggatg atgtggccaa
     4321 ttacgcagat tttgtatatg ttcttgacaa aggtaaaatt attttatcag gaaaacccaa
     4381 aacaattttt caacaagtca gtttgcttga gaaaaaacag ttaggagttc ctaaggtgac
     4441 taaacttgct caaaggcttg ttgatagagg aatacctatc tcttctttgc ctattacctt
     4501 agaggagcta agagaggtac taaaacatgg ataagctgat tcttggtcgt tatattcccg
     4561 gagattctct cattcatcgt cttgacccaa gaagtaaatt attagcgatg attatttata
     4621 ttgtcattat cttttgggct aacaatgttg tgacaaattt gttgatgttg acttttactt
     4681 tagcagttgt ttttttgtca aaaatcaagt tgtctttctt tttaaatggg gttaaaccaa
     4741 tgattggtat tatcctcttc acgactttat ttcagatgtt tttctcacaa ggaggaaaag
     4801 taatttttag ttggtggttc attagcataa ctgatttagg attgagccaa gctattttaa
     4861 tttttatgcg tttcgttcta attattttct tttcaacgct gttaacgttg acgacaacac
     4921 ctttaagcct atctgatgca gttgaatcat tattaaaacc tttaacaaga tttaaagttc
     4981 cagctcacga gatcggacta atgctttctt taagtttacg ttttgtgcct actttaatgg
     5041 atgataccac gcgtattatg aatgcacaaa gggcccgtgg agttgatttt ggagagggaa
     5101 atttaattca aaaagttaaa tccattattc ctattttaat ccctcttttc gcttcaagct
     5161 ttaaaagagc cgacgcatta gctattgcta tggaagctag aggttatcag ggaggagaag
     5221 ggcgaactaa gtatcgtcaa ttagactggc agctaaaaga tagtttagct attggaatag
     5281 tttctttatt gggtttgcta ctttttttct tgaaaacctc tttgtaatat tttttagcac
     5341 aaaacttttc tttattagta agaagtgaat tgtacctcaa aagttagata caacatctaa
     5401 tgattgaggt acttttcttc atttgaggaa ttgaaaaccg ctatctcaac tatattaatc
     5461 atcccaacaa taacaaatcg aacgaaatct aaaaggactg agtcctgttc aatacagaac
     5521 taaattctta gtttaatgat attatcccgc tttttaatgt tgctaagaag aagagtttta
     5581 tgatttttta caacttctta taatatcaat gtagttgtaa tatttttaat atatcttcaa
     5641 taacgaggta ataattaaaa gatattatgg ataacggaga caagtgaggt attattgaat
     5701 gtttaagaaa gaaaatttaa aacaacgtta ttttaatttt ggattagtag cgttagctct
     5761 aacaatatta gccatcattt ttgccttctc aagtaaaaat gctgatacta agtcttatgc
     5821 taagaagtca gaaagtaaaa tggtaacaat cgacaaggtt ccaaaaaata atcatgctat
     5881 tactaaagaa gaaagcaaag aaaaagcaaa gagcattgct tcggagccta ttcccacagt
     5941 agaaaactct gtagctccga cagtaacaga ggaagcaccg gttgttcagc aagaagtgac
     6001 tcaaactgtt cagcaggtat cttcagtagc ctataatcca aacaatgtgg tactttccaa
     6061 tggaaatact gctggtattg taggaagtca agcggcggca cagatggcag cagcaacagg
     6121 tgttccacaa tcaacttggg aacatataat tgcgcgtgaa tctaatggaa atcctaacgc
     6181 aactaatgct tctggggcat cagggttgtt ccagacaatg ccaggttggg gttctacagc
     6241 aacggttgaa gatcaagtca atgcagcctt gaaagcctat agtgcacaag gtttatcagc
     6301 ttggggttac taaataaaca tagctggaag aaataaaacc tattgttagt atttactaac
     6361 aataggtttt tggtatttaa ttgtctttat gatttgtagt gatgaattag tctcaaaaca
     6421 aaacattgcc ttatcaggga atatagctcg tattatttag gaaaactgac cagcacttaa
     6481 gtgcctctga tatttaaggc agttatactg ataacaagca ggaaaaaaac tcttaaatgt
     6541 ttataaggtc attagcaatt ttcagtattt tacactacta cacttttgag tttcaacaat
     6601 ttactaggag ttgtttctaa ggggaaacaa catagcttga tagc
//
LOCUS       DAUCWO010000074         6255 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUCWO010000074 DAUCWO010000000
VERSION     DAUCWO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.074"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            7..294
                     /locus_tag="VGY20_001524"
     CDS             7..294
                     /locus_tag="VGY20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ4274356.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            284..619
                     /locus_tag="VGY20_001525"
     CDS             284..619
                     /locus_tag="VGY20_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ4274357.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFEADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            771..1241
                     /locus_tag="VGY20_001526"
                     /pseudo
     CDS             771..1241
                     /locus_tag="VGY20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            1204..1704
                     /locus_tag="VGY20_001527"
     CDS             1204..1704
                     /locus_tag="VGY20_001527"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ4274358.1"
                     /translation="MAALRKGELCALKWADIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETLSILQEWKAICQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHA
                     DYINNILSKVIKQHSLKKISPHGFRHTHATLMIEIGVDPVNTAKRLGHAISQMTLDTY
                     SHATAV"
     gene            complement(1872..2264)
                     /gene="rpsI"
                     /locus_tag="VGY20_001528"
     CDS             complement(1872..2264)
                     /gene="rpsI"
                     /locus_tag="VGY20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ4274359.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2285..2731)
                     /gene="rplM"
                     /locus_tag="VGY20_001529"
     CDS             complement(2285..2731)
                     /gene="rplM"
                     /locus_tag="VGY20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ4274360.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(2949..3155)
                     /locus_tag="VGY20_001530"
     CDS             complement(2949..3155)
                     /locus_tag="VGY20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274361.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(3152..3850)
                     /locus_tag="VGY20_001531"
     CDS             complement(3152..3850)
                     /locus_tag="VGY20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274362.1"
                     /translation="MFDSKQNLAKSQWMFIIIIAVMDLVVLVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQMRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILMGMIATGMSLVTIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(3986..4846)
                     /locus_tag="VGY20_001532"
     CDS             complement(3986..4846)
                     /locus_tag="VGY20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4274363.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(4943..5461)
                     /locus_tag="VGY20_001533"
     CDS             complement(4943..5461)
                     /locus_tag="VGY20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ4274364.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     SQLKDFMSQLDR"
     gene            complement(5465..6211)
                     /gene="rlmB"
                     /locus_tag="VGY20_001534"
     CDS             complement(5465..6211)
                     /gene="rlmB"
                     /locus_tag="VGY20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ4274365.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
BASE COUNT         1922 a         1324 c         1143 g         1866 t
ORIGIN      
        1 cataaaatga ctacagtaaa aaaaaacaga gcggttacct ttcaagctaa taaagaattg
       61 gtaagcgaag caatgacagt attaaacaaa aaaaatttaa ccttatcatc tgctttaaga
      121 ttattcctac aaaatgtcgt tgtcacaaac gaggttgact tattgacgga agaggagtta
      181 gagaaagaaa aacttttcaa gcaatttcaa gctgaaatca acaaaaatat tgaggatgtt
      241 cgtcaaggga agttttatac ctctgaagaa gtgagagctg aacttggact ataagaaata
      301 tcagattatc tatgctcctg atgttttaga gaagctaaaa gaaattcgtg attatatttc
      361 tcaaaactat tcctcaacat caggtcagcg taaaatggag caaatcatta gcgacataga
      421 aaaactcgag gttttcccag aagttggttt tgaggctgat gaaaaatatg gttcaaaaat
      481 cagccattat cacagcacta aaggttacac cttaagtaaa gattatattg tcctatacca
      541 tatcgagggg gaagaaaata gggtcgttat tgattacttg cttcctactc aaagcgacta
      601 tataaagtta ttcaaataac acgttaaaag ccttagcagt cttgctaggg cttttttggt
      661 agattaccaa ttaactagga gcaaaatgag ttcgggctgt ttagtagata ttccacaaag
      721 aagccaaaga ctacgaagct aggctaatct accttaaggc ttcaggaaac ttggaggagt
      781 tcgccaagcc aactcataaa acttatagcg agatattcga gaaatggtat caagcctatc
      841 aagatacggt agagccaacc acggcttctc gtactcttga tctgtttcga cttcacattc
      901 tgccagtcat gggagaattg cccattaaca aaacctcccc tttagactgc tagaacttca
      961 tcaccgataa agcaaagacc tttaagaata tcaagcaaat caagtcctac actgaaaagg
     1021 tctttgattt tgccatcaag atgaaactgc tcaagcataa ccctatggct gaaatcatta
     1081 tgccaaagcg gaaaaagaca cgaatagaaa actattggac ggttcaagaa cttcaggagt
     1141 ttttagccat agtacttcag aaagagcctt acaagcacta cgcactattt cggctactag
     1201 cttatagcgg ccttaaggaa aggcgagcta tgcgctctta aatgggctga tatagacttt
     1261 cagagtgaga cactaagcgt caataagagc ctaggacgat tagacggaca ggcaatcgaa
     1321 aaaagcacta aaaatgattt ctcagtaaga aagattaagc tagacagcga aactctttcc
     1381 attcttcaag aatggaaagc catctgccaa aaagaaaagg cacaactagc agttgcacct
     1441 ctttcaattg agtctgactt tatatttaca tactgtacta gagaagggag tattgaacca
     1501 cttcatgcag actatattaa caatatctta tcaaaggtca ttaagcaaca tagtcttaaa
     1561 aaaataagtc ctcacggctt caggcatacc catgcaaccc tgatgattga gattggagtt
     1621 gacccagtaa atacagccaa aaggttagga catgctatta gtcaaatgac tcttgataca
     1681 tacagtcatg ctactgcagt ctgagaggac aaatcaatca aacaatttgc taactatctc
     1741 aaaaataact aaataaacgt attaaaccaa atcaaaagtt ttccgaaacg gtatcaaaag
     1801 caaaaaacga caaaaaaagc accttgcaag agacttgcaa aatgcttgta atcgttgatg
     1861 taaagctgtt cttaacgttt tgagaattgt gaagctttac gagctttctt aagacctggt
     1921 tttttacgtt caaccatacg tgcgtcacgt gtaagaaggc cagcgcgttt caatgaatcg
     1981 cggaagtctg ggtctacttg aagaagcgca cgagcgatac catgacggat cgcacctgat
     2041 tgtccaccgt aaccaccacc aacaacgtta acgaaaacgt cgtatgaacc ttcagttgat
     2101 gtaactgcaa aaggttggtt gatgatcaaa cgtaggtcag catgtgggat gtattcttct
     2161 acatcttttt tgttaacagt gattttacca gtacctggaa ccaaacgaac gcgtgcaaca
     2221 gcgtttttac gacggccagt acctgcatat tgtgcttgtg ccattattta atgctccttt
     2281 cctcttagat aagtcctgag atgtcaagta cttctggttg ttgtgctgcg tgagtgtgct
     2341 caccgccaac gaagactttc aatttcatac cttgtgcacg tccaagagtg ttatgtggaa
     2401 gcatgccttt aactgatttt tcaatcaagc gaactgcgtt tttagagcga agttcaccag
     2461 cagtgattga tttcaaacca cctgggtaca ttgagtgagt gtagtaaact ttatcagttg
     2521 cttttttacc agttaattta actttttcag cgttgataac gataacaaag tcacctgtat
     2581 cagtgtgtgg tgtgaaagtt ggtttgtttt ttccgcgaag tacgctagca actactgcag
     2641 aaagacgtcc aagtggcaca tcagttgcgt caacaacgta ccatttgcgt tcaacttggc
     2701 ctggtttagc catgaaagtt gttttgttca tgatttctcc tatacgaatt cgtaatattt
     2761 gtttacaagg tgatgggtgt tccgtcccat cgaaggtatt tggaaggttc cggggccttt
     2821 caaatggggt aaacaatacc gcctactatg atatcaaact taagaggctc cagtcaagtg
     2881 ctttagctga ttttattgga ttattttttc tttcgattta ggagaatggc agagctgctc
     2941 tgctggtgtt aacgagaatc atcagcttcc caaaaaaggt cgtctaaggt cttatcaagc
     3001 actcgacaaa tggcaatgca aagattaatc gttgggttgt aatcaccttt ttcaacagcc
     3061 gcaatggttt gtctggagac gcctaccaaa tctgctagag cttgttgaga caaatctttg
     3121 cctgcacgcg ctgcttttaa tttgaggttc ttcattcatc tgcctcttta gcatccagat
     3181 aatgacgata gaaaatacta gctcccatac taaggagcgt tatgcctaac accattaaag
     3241 atccactgcc gccagccttg aaaaagcttc ccattttaag ttgccctgcc atcatctctc
     3301 ttaccgccaa acctatagtg actaagctca ttcctgttgc tatcatcccc attagtattc
     3361 cttgttttcc aaagcgccta tccacaaatg gcgatacccc gttgaggtta caatatgtcg
     3421 tttgaactgc caaccctccc cataaagcca acgtaaagag gaagcttgca gataccagtc
     3481 cctctgtgaa tcggctaata aagagtgaca gccagataac cacaatcata aaccaaaacc
     3541 caatagtgta tcctttgaga agaatctgtt tttggcgttc atcatatttg ggcaacttct
     3601 gatcgtccct taccttagaa tctagtactt ccttgctgtc tagtctcatt tggagctttt
     3661 tcgctgtctt tagcccccat accaacaata tgagaaagct agtcaatata accgctacgc
     3721 ctccaactaa gtaacgttga aaactggttt tagccagcag tgtcgctact aacacaacca
     3781 aatccatcac tgctattatg attataaaca tccattgtga cttagcgaga ttttgtttgc
     3841 tatcaaacat gattcgtttc tcctttaaat ctttacatga cataatacaa attattttgg
     3901 atattttgtc aaatatattt tacattcttg attgatttaa gcagcaaaaa aggattgagg
     3961 atttctcttc aatccttata agggattaac gggaattctg cccaatcaca aaaactgcca
     4021 gggtgttagg accaacgtga gctgagataa ctggtcctag tggcatcata agaacatcgc
     4081 taatattctc atgcgccagt agctcttctc gcaacttttc ggcactaccc tgatcgcttg
     4141 tataagagac aataaccgtc gaatccgcaa tatctttttc cacttgagcc accatttctt
     4201 tgatggcttt ttggcgtccc cgaatttttg caatgggcac caatttccct tcctcatcaa
     4261 tccacaaaag aggtttgata ctggctaaac tgcctagaaa agctgaacct tttgacagac
     4321 gcccaccacg catcaaatga aaaagatcat ctactaagaa ataggtgcgc agtcgaggga
     4381 tcactgcttc aacaatatct ttagtctcca gtaaattttt gccactatct ctggcttctg
     4441 ctgctaaaat ggttagatag ccttctcctc ctgcagcagc caaggtatca acaatttcaa
     4501 tcaccgcatc gggataatct tctcgaacaa gatcgcgtgc cattagagcg ctttgatagg
     4561 tacccgataa aaccgaagaa aaagcaagat aaagcagtgc tttgttgttc ctagcatgtt
     4621 cacgaaaaac cttctcaaac tctcccacat taatctgact ggtttgagga tgactgccag
     4681 ctttcatctt tttcaaaagg taatcactac taatacggtt aggaccgacc gtttcataaa
     4741 cctctccatc gcacaaaatc gttaatccta taaggacaat atcatgatct tctgcccagg
     4801 tttgattcaa atcagcggtt gaatctgtca ttattgtaaa ggtcattctt acttctcctc
     4861 atccagtttt ttagtttgac aatcaaataa ttattgtttt attttatcaa aaaaacccgc
     4921 acttggcgag tcgtaattct cattatcgat ctaactgaga cataaaatcc ttcaattgac
     4981 taagctgtcc ttggtcaaaa ggccgcagtt taggcgtttt gagatcaata tctctagaca
     5041 ttttatctaa atcagctttg acagtatgaa ctctttgctc taattctctt gcagtgacac
     5101 gaagagctcc ttgagaaaaa atcgtccatt gttcatttaa atcactcgta gctacttcta
     5161 ccatatgtat agccgtattt aactctgctg ccatgcgctc aatgtagcta tctgctgttt
     5221 cgtcttcctc tgtaaatacc actgagatat aatactggtc atatcgttgc cttagccccg
     5281 gtacatattg ggcatcaaaa acacaaataa tattaatatt ctcaaaatgg gcataatgat
     5341 taagttttgt taaaagtgtg ttacgtgcct gatcaagctg atttgtctta aacaactgac
     5401 gggttgattg ccaaaaggca atcatattat acccatctac taataatatc cgttttttca
     5461 taatttagcg cctatttcgg aaaacttcat acatgagaat agctgccgct acgctagcat
     5521 ttaagctctg tacgtgacca ttcataggaa tcgtaatcat ttcatcaact tgctttttga
     5581 tattagtaga gatgcctttg ccttcgttac caatgactaa agcaagttta ccattagtgt
     5641 tccagcaatc agacggtgtt ccattcatgt ctgtgccaaa aatccagaat cctcttgctt
     5701 tcaatttatc tagagtttga ctaaggttag ttactctcgc tatcggaata tgttcaacag
     5761 cacctgtaga tgtctttgaa accactggag tcacaccaac agaacgatgt ttaggaataa
     5821 tcactccaca tacatttgta gcatcagctg tccgtaaaat cgatcctaaa ttatgaggat
     5881 ctgtcagacc atctaaaata agaatcaaag gatttgcttc ttgctctgct atttcgagga
     5941 tctcatcaac atcagtatag gcaaaagctg atactctcag aacaaaacct tgatgcacag
     6001 ctccatcagt catttgtgac aaggtttttt taggcgtcca tgaaatagcg accttttttt
     6061 gtgtcgctaa gcttttgatg ttatccactt tctttcctct taaatcctct tggatataaa
     6121 gcttatttcc tgtatttgct tgaaggcttt ctgtaacggc atgaacacca tagacgatat
     6181 cgtttgtttc aatagtatct ttatcttcca tgagtttatt ataccatgaa tgtttcaaaa
     6241 taaatgcata gactc
//
LOCUS       DAUCWO010000075         6089 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUCWO010000075 DAUCWO010000000
VERSION     DAUCWO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.075"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            213..1427
                     /gene="sstT"
                     /locus_tag="VGY20_001535"
     CDS             213..1427
                     /gene="sstT"
                     /locus_tag="VGY20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ4274366.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGRQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(1482..2156)
                     /locus_tag="VGY20_001536"
     CDS             complement(1482..2156)
                     /locus_tag="VGY20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ4274367.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGVEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(2166..3557)
                     /locus_tag="VGY20_001537"
     CDS             complement(2166..3557)
                     /locus_tag="VGY20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ4274368.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFGLEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(3627..4340)
                     /gene="rsmG"
                     /locus_tag="VGY20_001538"
     CDS             complement(3627..4340)
                     /gene="rsmG"
                     /locus_tag="VGY20_001538"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ4274369.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVITARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            4490..5047
                     /locus_tag="VGY20_001539"
     CDS             4490..5047
                     /locus_tag="VGY20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ4274370.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            5094..5990
                     /gene="htpX"
                     /locus_tag="VGY20_001540"
     CDS             5094..5990
                     /gene="htpX"
                     /locus_tag="VGY20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ4274371.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
BASE COUNT         1925 a         1086 c         1172 g         1906 t
ORIGIN      
        1 atactaatcc gaagattcta acaattttaa ttatattatg tgtcaaaata acaacaaagg
       61 ttattatatc taagattact tcgatagtca atagaaattg actgaaaatt cttagcacaa
      121 aaaatggtta agatatgtag cttattcgat ttgacgaata gatagaatta tattattata
      181 aaaaacatgt aactttagta aggatgtaac gtatgaaaaa aatatatgat ttatgggtta
      241 gggttagtct aatcaaaaaa ataggtatcg gtgttgtaat aggagtcatg ttgggcattt
      301 tagctcctga tcttacaggt tttagtattt taggaaagtt atttgttggt ggtttaaagg
      361 ctatcgcacc tttgcttgta tttgctttgg tttcccaagc catctcccat cagaaaaaag
      421 ggagacagac taatatgaca ttaatcattg ttctgtattt atttggtacc tttgcgtcag
      481 ctttagtagc agtactgaca gcttatctat tccctttgac gttagtgtta aatacacctg
      541 taaatacaga attatcacca cctcaaggtg ttgctgaagt ttttcagtca cttctattaa
      601 aattagtgga taatccgatc aatgctttag ctactgccaa ttatattggt gtgctatcat
      661 gggcaattat ttttggacta gcattaaagg ctgcaagtca agaaacaaag catcttataa
      721 aaacagcggc agaagttact tcacaaatag ttgtttggat catcaatctg gcacctattg
      781 ggattatgag tctggtgttc actacgattt ctgaaaacgg tgttggaatc ttatctgatt
      841 atgctttttt gatactagtt ttagtaggaa caatggtttt tgtcgcctta gtggttaatc
      901 cacttattgc ggttttgatt acacgacaaa atccttatcc ccttgtcttg agatgtttac
      961 gtgagtcagg tcttacagca tttttcacac gaagttctgc agccaatatt ccagtcaata
     1021 tgcaattatg ccaaaaaatt ggattgagta aagatactta ttcagtgtca attccacttg
     1081 gagcaactat taatatgggt ggggcagcaa ttaccatcaa tgtcctaacg cttgctgctg
     1141 tacacacttt tggtattcct attgattttc taacagccct cttacttagc gttgttgctg
     1201 ctgtctctgc ttgtggtgct tctggtgttg ctgggggatc gttgttactt attcctgtag
     1261 catgtagttt gtttggcatt tcaaatgatc ttgcgatgca ggtggttggt gttggtttta
     1321 ttgttggtgt tatccaggat tcttgtgaga cagccttaaa ttcgtcaacc gatgttcttt
     1381 ttacagctat tgctgaaaat gctttttgga aacgtaaaaa agcctagcta ggtttaacat
     1441 agtaaaaaac atccttggaa aacgccaagg atgttttttt attatttaag atagccaaga
     1501 taatcaaatt tttcaaaggt gtggtcatta gcgatggcca caatgatagt attcggttct
     1561 aaaggttcaa aaggtttgac attagtatct aaggttttaa cttctttttg acgcatgcca
     1621 atcacattta actcatattt acgacgaaca tctagttcgg aaagagattg tccttcccag
     1681 cttttaggaa tgacaaattc aatcatcgaa ataccatgtt ccaaatagat gatgctttca
     1741 atatgacgtc ttaacagatt tgaagctaca cgttttccag aatcacgttc aggggtgatg
     1801 actttagttg ctccgatgcc atatagaact tcttcaaaaa ttttattttt agccttggca
     1861 ataatggttg gaacaccgag ttttttacag tgcatgactg caagaacaga agattccaag
     1921 ttattccctg aagcgataac gacagtatca caatgttcaa cccctacagc taatagaaat
     1981 tctttgtcag taatatctcc aacagcggct ttagtgacca agtcggccac ctctttgaca
     2041 tgactttctc tgatatcaat agcaataacg tcttgatcaa agttacttaa ttctctggca
     2101 acagtacggc caaaaatacc aaggccaaga acgccgacag ttttacgttt taacatgttt
     2161 tctccttaac ccactagaat atcagtagtg gcatattgaa tggttttttc ttttcgttga
     2221 atcagactaa tgaggacagt aattgggcca acgcggccga cgaacatcaa gacaatgaca
     2281 ataagacgtc cagcagtaga caattgtggt gtcaaatcca tagatacccc aactgtcgcg
     2341 atggcagaaa tagattcaaa taagagtggt ataggagcga tatgaggctc cacgctaagt
     2401 aataaaataa aacctatcat aagaactgca aaaaagaaga taagaactgt catggtctgc
     2461 ttaattgtct tatttgcgat aattcggtta cgaaaggtaa cttctgattg ccctgaaagt
     2521 tccgctttaa agagtaagaa agtaatggca gcagtcgtca ctttaatccc accagctgtt
     2581 ccaccaggag caccaccaat aaccatttga atcatataaa gaatgttagt tggtgctagg
     2641 gtatcgttat aagagattgt tgcaaagcca gctgttctca tagtgactgt ttgaaaaaat
     2701 gaaaccataa gttgctgatg taaactaaaa ttggcaatgg ttttgctgtt atctttttct
     2761 aaaaaccagg ttaagaaggt tccaagaaaa agaataacag ctgtggtttg tagtactaga
     2821 cgagactgat tggataattt acgaaaagtt gccccataac aatgaggtct ttcaaaaaag
     2881 tattttttaa aagctactcc taaatcaacc caaaccgcaa aaccaagtcc accagagata
     2941 atcaagaacg taatgataac atttaatgta ggatttaaca taaagtcttt taaactagaa
     3001 gatcctaaat tgtcaaaacc agcgttacaa aatgccgaca cagctaaaaa aatactattg
     3061 aagataccat ttttccaccc gaagcggggg ataaagtcaa tcatgataac tatcgcagca
     3121 aaggcttcta gaccaaaggt tactttataa gcaaaaaaaa ggtagtgctt taaatctttg
     3181 ctatcacctc gatttaacgc tgattggagc aaggtttgat cgctcaatcg catttttcgc
     3241 ttgagggcaa aagtactcac agctattaac gtcaccaagc caagaccacc aatctgcatt
     3301 agtgccatag caatggtttg tccgataccg ttatacactt cagcgacggg aacaacagaa
     3361 agtccagtca cacaaaccat ggaaaccaca ttaaagaaat gatcaagata gacggtatta
     3421 ggaccatttt gataatgggt aaagggcatg gataacaaga gagtcccaat cagaatcaca
     3481 atggcaaaac taaatgttaa gcgctgtgtg actgaaagag atttgataaa agagcgtttc
     3541 atgtttcctc cagacataat atctcctact ataataacaa aaataagtta aaaaagctaa
     3601 gtttcatctt agcctttggt actattttat agaggttttt tgttaggcaa gccagctttt
     3661 cttggatact tgttaggtgt ttcctttttc ttctcaacaa tggtgatgaa gcgagaatct
     3721 ccatttggca attggtagct atggttttta atgactttac caaaaagaag acaaagagca
     3781 ttcttagctt cttctaactc ttggtcagca gcttgtgcct ttaaggcgat taattttcct
     3841 ccaattttta aaaaggggat ggttaattca gatagaacct gcatccgagc aaccgctctg
     3901 gcagtaatga catcaaattg acctcgaaag gccttgtcct gtccgaaatc ttctgctcta
     3961 ccatgaaaaa aatggacatt ttccaagccc aattcctgag caaggagtgt cagaaaagaa
     4021 atccgcttat taagagaatc aataatggta acctcaaggt ttgggaaaag aattttcatg
     4081 ggtaggctag gaaaaccagc tccagctcca atgtccaata atttgattgg ttcattagct
     4141 aaaaagccct gtaaaatagg agcgatggaa tcataaaaat gcttgaggta aacctcattt
     4201 tcttctgtaa tagctgttaa attaatttta gtgttccatt ccactagtaa tttgaagtag
     4261 gtgtcaaatt gttctttttg tttcgatgat aaggaaaaac catcttcctc tagtgttcgg
     4321 taaaagtctt ggggtgtcat ttgtatcctc tgtttttgat agttgttacc attttatcat
     4381 aaaatagccc ataacaaaat ccaataaatc atcagaaata acacctcatt acttgctatt
     4441 agcctagaaa ttgctataat tgagacacaa agattaaagg agacccacta tgccaacagt
     4501 tttgattatc ttagtcgttt taggagtgct tgcgctatgg ttaatgatta gttacaatag
     4561 cctggttaaa tctcggatgc acacaaaaga agcttggagt cagattgatg tgcaattaaa
     4621 gcgtcgtaat gacttgattc caaatcttat tgaaaccgta aagggatatg ctagctacga
     4681 gcaaaagaca tttgaaaaaa tcactgattt gcgtgcgcgt gttgcgaatg cctcaactcc
     4741 tcaagaaacc atggcggctt ctaacgaatt gagtaaacaa gtgaccagtt tgtttgccgt
     4801 tgctgaaaat tacccagact taaaagctaa cgaaaacttc ttgaaattac aagaagagtt
     4861 gaccaatacg gaaaataaaa tctcatattc tcgtcaactc tataattcaa caacgtctaa
     4921 ttacaacctt caattagaat ctttcccaag caatatcgct ggtaaattat ttggttttaa
     4981 accaagtgaa ttcttacaaa caccagaagc tgaaaaagaa gttccaaaag ttgaatttaa
     5041 cttttaaata tgaaattatc atctagtagg caatcataag agaaaggaga attatgcttt
     5101 atcaacagat ttcacaaaat aagcaacgaa cagttgtttt attagttgtt ttcttcgctc
     5161 tcttagcact tattggagct tcagcaggct atctgctgtt agataattat gccatgggct
     5221 tggttcttgc tcttgtcatt ggggtgattt atgctaccag tatgattttt caatccacca
     5281 gtcttgtaat gagtatgaac aatgctagag aagttacaga aaaagaggct ccaggcttct
     5341 tccatattgt cgaggatatg gctatggtgg cccagattcc gatgccgaga gtttttatta
     5401 ttgaagatcc ttctttaaat gcttttgcga caggatctag ccctcaaaat gctgctgttg
     5461 cagcaacgac aggtttactt gaagtcatga atcgtgaaga acttgaaggt gttatcggcc
     5521 atgaaattag ccacatccga aattatgata tccgtatttc gactattgct gtagctttgg
     5581 cgagtgctgt taccgttatt tctagtatcg gtgggcgtat gctatggtat ggtggcggtt
     5641 ctcgcaggca aagagatgac ggagatgatg atgttttaag aatcattaca ttacttttgt
     5701 cgttattatc tttgctttta gcgcccctag tagcaagttt gattcagttg gctatttctc
     5761 gtcaaagaga atatctagct gatgctagtt ctgttgaact aacaagaaat ccccaaggta
     5821 tgattaaggc tcttgaaaaa ttgcagttat ctcagccaat gaagcatcct gttgatgatg
     5881 ctagtgcagc cttgtatatt aatgagcctc gcaaaaaaag gagcttcagt tcattattca
     5941 gcacccatcc tcctattgag gagaggattg aaaggttaaa aaacatgtga gcgtttgggg
     6001 tttttgctca agcctcactt ttttatatct ttgggtagat aatctaaaat tgcaggaatt
     6061 atcaaaagta tctgaacaaa aagaaaaaa
//
LOCUS       DAUCWO010000076         5935 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUCWO010000076 DAUCWO010000000
VERSION     DAUCWO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.076"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1134)
                     /locus_tag="VGY20_001541"
     CDS             complement(<1..1134)
                     /locus_tag="VGY20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ4274372.1"
                     /translation="MVIFDLKHVQTLHSLSQLSISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHF"
     gene            1499..4999
                     /locus_tag="VGY20_001542"
     CDS             1499..4999
                     /locus_tag="VGY20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4274373.1"
                     /translation="MWREKINDTKKSYKLSFLLSLTGFILGLLLVFIGLSRVSVGHAE
                     TRNGANKQGAFEIKKVDQNNKPLSGATFSLTPKDGKGASVQTFESGSDGKIDAKDIKP
                     GTYTLKEDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISRAGSKDVSQSLQLE
                     KSTMSVVSKYGEQEKTSNSADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDQR
                     LNPKGISQDIPKIIYDSANSPLAIGKYHAENHQLIYTFTDYIAGLDKVQLSAELSLFL
                     ENKKVLENTNISNFKSTIGGQEIKYGGTVNVIYGNEKNQKDNYVANGLSNVGGSIESY
                     NTQTGDFTWYVYVNPNRTNIPYATMNLWGFGRLTDKQAFSTDSNSSSAQLLNAEIYEV
                     PSDYHLPSSYGVDVTKLTLRTDITAGLGNGFQMTKRQRIDFKDNLKDGKRFVVKVTGK
                     TDQSGKPLVVQSNLASFNDQFAVKSSNPTNNVYFQNEIALSPSKGSGSGTSEFTKPSI
                     TVANLKRVAQLRFKKMSTDNVPLPEAAFELRSSTSDSQKVEAKSNEQGEVHFKDLTSG
                     TYDLYEKQAPKGYQQVTEKLATVTVDTTKPAEEIVKWKKTHPFVKVEVNKEVTIVNHK
                     ETLTFSGKKIWENDRPDQRPVKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYD
                     DKNQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGS
                     KIIDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEF
                     KNLKKYNGTGNDIIYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPNLEIEETLPL
                     ESGASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGA
                     TMELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQV
                     TVNGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLII
                     GGQGEVVDTTEDTQRGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDS
                     GCKTEVEDTKLVQSFHFDNKEPESNSEIPKKDKPKSNTSLPATGEKQHNKFFWMVTSC
                     SLISSVFVISLKSKKRLSSC"
     gene            complement(5265..5930)
                     /locus_tag="VGY20_001543"
     CDS             complement(5265..5930)
                     /locus_tag="VGY20_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274374.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKTLVDNDKNIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTEITYEHHY
                     WDGTVHSEKRRNTQWHYNWEKQKTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEDYKNKWNKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         2023 a         1003 c         1191 g         1718 t
ORIGIN      
        1 aaaatgagta gtgtcataaa aatataagct tttagccact tctccaacag gaattcctcg
       61 ttttaagagt agttgagctt ctttgatttt tgtacttaga atataatgtt gtaaggaaac
      121 atttgagtat tttttaaaga ctgaacgtaa gtgagattcg gaaacatata ggcgcttagc
      181 gatatcagat acctttaatt tggaatacag gtgactatcg acataatgaa gcacactatt
      241 gtacatttga cgtttatcag aaatactttt attggttaat gattcggaaa aatggatgat
      301 agctgcacag cgaattctca aaactttgag caagtcactc gcttcttcat ttaaggccat
      361 gtagtatttg acaaggctat aatgctttgc tacgtcagta ttttctacaa aactgaattc
      421 cagtaaccta gttaaataga taacagtgag gttcttttcc gcccttagag cagaaataga
      481 agatgaggtg atacttgata aggggatgcg attgatttct tgcttgagca attgtggatt
      541 gcctagcttt accagatcca taatatgatt taggtgctct aaaacaggcg gtagttggct
      601 agatgctttg ctaatatgct taaggcgatg aatgatatca ggatcagcag tcatctgttt
      661 tgaatgctgc aacaattgat gaattgtttg ttggcaagtc gtctcaaatt gtgtgtcaaa
      721 aaaagcgtca atagctatca aaaggttccg cacattatta attggaaaat gtgggacaag
      781 tgccatatag gacaagagct cttctttgct acgatgagaa gaatgtttta aaaaattatt
      841 agctaattgt tcctgatagt ctttattaag tgaataaggg tagaaaggtc caatggcaat
      901 aatgtagtga caaagaggaa aaatcataaa ggactcttca aataaaccct cataaaaaat
      961 aacatcttgt gcagcttgag gaatagctag atgctttaaa aattgatagt aacataataa
     1021 atagtcgtca ttaccatata cttgaataag tgccttatct tgtgacatca ctgaaataga
     1081 taattgagac aagctgtgta atgtttgcac atgttttaaa tcgaatataa ccatttgata
     1141 gattgccccc atccttagtt gtatcatttt ccttagactt gtaaaagctt tagtaaatgt
     1201 taatgatgaa aattcctatt tggtaggcac actaaaaggt cttcctaata gtaatctctc
     1261 tctccatcat ctcctgcctt aattatacta tgaatagcta agcagataaa gataaaggac
     1321 ttgtacgtta agcgcttgaa aaagaaagag ttacagataa tgacataaaa aacgctaaaa
     1381 aaccatcaaa ataaatattc taaccataag atgtagactt gacaactgaa aatggtaaaa
     1441 taactatttg acagttgtcc tgtagtcttt agttttggac ataggctgtc gcttatgaat
     1501 gtggagagag aaaataaatg acacaaaaaa aagctataag ttaagcttcc tgttatcctt
     1561 aacaggattt attttaggtt tattattggt ttttatagga ttgtccagag tatcagtagg
     1621 acatgcggaa acaagaaatg gagcaaacaa acaaggagct tttgaaatca agaaagtcga
     1681 ccaaaacaat aagcccttat cgggagcaac tttttcactg acaccaaagg atgggaaggg
     1741 agcatctgtt cagacctttg aatcaggttc agatggcaag attgatgcta aggatattaa
     1801 accagggact tataccttaa aagaagataa agcaccagat ggttatgata aaaccagtcg
     1861 gacttggaca gtgactgttt atgagaacgg ctataccaag ttggttgaaa atccctataa
     1921 tggggaaatc atcagtagag cagggtcaaa agatgttagc cagtctttac agttagaaaa
     1981 atcaacaatg tctgttgtat ccaagtacgg tgaacaagaa aaaacgtcaa actcagcgga
     2041 tttctaccgc aaccatgccg cctattttaa aatgtctttt gagttgaaac aaaaggataa
     2101 atctgaaaca atcaacccag gcgatacctt tgtgttacag ctggaccaac gtctcaatcc
     2161 taaaggtatc agtcaagata tccctaaaat catttacgac agtgcaaata gtccgcttgc
     2221 gattggaaaa taccatgccg agaaccatca acttatctat actttcacag attatattgc
     2281 aggtttagat aaagtccagt tgtctgcaga attgagctta ttcctagaga ataagaaagt
     2341 gttggaaaac accaacattt caaattttaa gagtactatt ggtggtcagg agatcaaata
     2401 cggtgggaca gttaatgtta tttacggaaa tgaaaaaaat caaaaagaca actatgttgc
     2461 taacggattg agcaatgtgg gtggaagtat tgaaagctac aatacacaaa caggcgactt
     2521 tacttggtat gtttatgtca acccaaatcg taccaatatt ccttatgcga ccatgaattt
     2581 atggggattt ggaaggttaa cagataaaca agctttctca actgattcta atagcagtag
     2641 tgctcagtta ttaaatgcgg aaatttatga agtgccttct gattaccatt taccatcaag
     2701 ttatggggtt gatgttacaa aacttacttt aagaacggat atcacagcag gcctaggaaa
     2761 tggttttcaa atgaccaaac gtcagcggat tgattttaaa gataacctta aagatggaaa
     2821 aaggtttgtt gttaaagtaa cagggaaaac agaccaatct ggtaagccac tagtcgttca
     2881 atccaatttg gcaagtttta atgaccaatt tgctgtaaaa tctagcaatc caactaataa
     2941 tgtctatttc caaaacgaaa ttgccttgtc tccttctaag ggtagtggtt ctgggacaag
     3001 tgaatttact aagccctcta ttacagtagc aaatctaaaa cgagtggctc agcttcgctt
     3061 taagaaaatg tcaactgaca atgtgccatt gccagaagcg gcctttgagc tgcgctcatc
     3121 aactagtgac agtcaaaaag tagaagcaaa atcaaatgaa caaggagagg ttcactttaa
     3181 ggacctgacc tcgggcacct acgacttgta tgaaaaacaa gcacctaaag gctatcagca
     3241 ggtgacagag aaattggcga ctgttactgt tgatactacc aaacctgctg aggaaatagt
     3301 caagtggaaa aagacacatc cgtttgtaaa agtagaagtt aacaaagaag tcacgattgt
     3361 caaccataaa gaaaccctta cgttttcagg gaagaaaatt tgggagaatg acagaccaga
     3421 tcaacgccca gtaaagattc aagtgcaact gttgcaaaat ggtcaaaaaa tgtccgataa
     3481 agttcaagaa gtaacgaagg ataacgattg gtcttatcac ttcaaagact tgcctaagta
     3541 cgatgacaag aatcaggagt ataagtactc agttgaagaa gtaaaagttc cagacggcta
     3601 caaggtgtcg tatttaggaa atgatatatt taacaccaga gaaacagaat ttgtgtttga
     3661 acagaataac tttaaccttg aatttggaaa tgctgaaata aaaggtcaat ctgggtcaaa
     3721 aatcattgat gaagacacgc taacgtcttt caaaggtaag aaaatttgga aaaatgatac
     3781 ggcagaaaat cgtccccaag ccattcaagt gcagctttat gctgatggag tggctgtgga
     3841 aggtcaaacc aaatttattt ctggctcagg taatgagtgg tcgtttgagt ttaaaaactt
     3901 gaagaagtat aatggaacag gtaatgacat catttactct gttaaagaag taactgttcc
     3961 aacaggttat gatgtgactt actcagctaa tgatattatt aataccaaac gtgaggttat
     4021 tacacaacaa ggaccgaacc tagagattga agaaacactt ccgctagaat caggtgcttc
     4081 aggtggtacc actactgtcg aagactcacg cccagttgat accttgtcag gtttatcaag
     4141 tgagcaaggt cagtccggtg atatgacaat tgaagaagat agtgctaccc atattaaatt
     4201 ctcaaaacgt gatattgacg gcaaagagtt agctggtgca actatggagt tgcgtgattc
     4261 atctggtaaa actattagta catggatttc agatggacaa gtgaaagatt tctacctgat
     4321 gccaggaaaa tatacatttg tcgaaaccgc agcaccagac ggttatgaga tagcaactgc
     4381 tattaccttt acagttaatg agcaaggtca ggttactgta aatggcaaag caactaaagg
     4441 tgacgctcat attgtcatgg ttgatgctta caagccaact aagggttcag gtcaggttat
     4501 tgatattgaa gaaaagcttc cagacgagca aggtcattct ggttcaacta ctgaaataga
     4561 agacagtaaa tcttcagacc ttatcattgg cggtcaaggt gaagttgttg acacaacaga
     4621 agacacacaa cgtggtatga caggccattc tggctcaact actgaaatag aagatagcaa
     4681 gtcttcagac gttatcattg gtggtcaggg gcaggttgtc gagacaacag aggataccca
     4741 aactggcatg cacggggatt ctggttgtaa aacggaagtc gaagatacta aactagtaca
     4801 atccttccac tttgataaca aggaaccaga aagtaactct gagattccta aaaaagataa
     4861 gccaaagagt aatactagtt taccagcaac tggtgagaag caacataaca agttcttttg
     4921 gatggttact tcttgctcac ttattagtag tgtttttgta atatcactaa aatccaaaaa
     4981 acgcctatca tcatgttaag ataagctgtt ggtgacaata gcgaaaaagg gtccacttat
     5041 tagtgggcct ctttttctgg aatcgatcgt ctcggttcac tagataagtc attttaagta
     5101 gttcaaaaag gcagagcctt aaaaggtttt ctgccttttt aatcttgttg ggcacttacg
     5161 aaagtatttc ggccacttca agcataataa aaagagtctg agcaatcatg ttgtctcaga
     5221 ctctgactgt aattatgcaa aagatgatgg actggcagct tcatttactc accgaactcc
     5281 cctttataac ttgggaatga tgctgctaat gggttgtttt gtttaatacc tgctataaac
     5341 ggtaatttat tccatttatt tttataatct tcttcaagct cagttttaaa cttccaaacc
     5401 attttatgag aaaaataagt gtgtgattga cggccatagg tgtctactaa aacgtgagaa
     5461 aattttccgt cgttttttag tttgtaaaat ttatctagag aaggactcca accagtataa
     5521 ccagggaatt tatcaaattc ttgattaaaa tttgtttttt gtttttccca attataatgc
     5581 cattgtgtat tcctgcgttt ttcagaatga actgtaccgt cccaataatg atgctcataa
     5641 gttatctccg taggagcttt tacaccctta ggaagaaaaa ctgagaaatc agggtaaacc
     5701 cattgttctt tagctgaatt aaaggttaca gtccattcga tatttttatc gttatcgacc
     5761 aatgttttag tatagtcgac atagttatca acatagtaat ttccctgcca gtaaaggtgc
     5821 actttacctt tctcatcaat gttatcatgg gcatgtactg atttatacga actaaacagc
     5881 atcgaagctg ctagtagtga agtgaagaga tatttattac atcgtttcat atcat
//
LOCUS       DAUCWO010000077         5699 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUCWO010000077 DAUCWO010000000
VERSION     DAUCWO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.077"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(408..716)
                     /locus_tag="VGY20_001544"
     CDS             complement(408..716)
                     /locus_tag="VGY20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274375.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNLSI
                     DTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVRID
                     "
     gene            complement(782..1233)
                     /locus_tag="VGY20_001545"
                     /pseudo
     CDS             complement(782..1233)
                     /locus_tag="VGY20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1338..1558)
                     /locus_tag="VGY20_001546"
                     /pseudo
     CDS             complement(1338..1558)
                     /locus_tag="VGY20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1577..1882)
                     /locus_tag="VGY20_001547"
     CDS             complement(1577..1882)
                     /locus_tag="VGY20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ4274376.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(1882..2184)
                     /locus_tag="VGY20_001548"
     CDS             complement(1882..2184)
                     /locus_tag="VGY20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ4274377.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLFQLKENA"
     gene            complement(2239..2415)
                     /locus_tag="VGY20_001549"
     CDS             complement(2239..2415)
                     /locus_tag="VGY20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ4274378.1"
                     /translation="MGQNVVMRQAPAGNIKPDKEKSVEKIDGIVSLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            2431..3324
                     /locus_tag="VGY20_001550"
     CDS             2431..3324
                     /locus_tag="VGY20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274379.1"
                     /translation="MTTMLDFPFHHEFIESLGRWTHILITLPKRHHGQVDTAIPRYLL
                     VTIREKTGIVKLTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQMIVSYFIQKCEVLE"
     gene            3324..3548
                     /locus_tag="VGY20_001551"
     CDS             3324..3548
                     /locus_tag="VGY20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274380.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            3539..5196
                     /locus_tag="VGY20_001552"
                     /pseudo
     CDS             3539..5196
                     /locus_tag="VGY20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
     gene            5186..5395
                     /locus_tag="VGY20_001553"
     CDS             5186..5395
                     /locus_tag="VGY20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274381.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(5497..>5699)
                     /locus_tag="VGY20_001554"
     CDS             complement(5497..>5699)
                     /locus_tag="VGY20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter permease"
                     /protein_id="HEQ4274382.1"
                     /translation="KDVDNDFLGRVFGIIFTITILFMPIGTGFFSVALNPNNSFNLFI
                     IGSCITILSLVFRILFKKYNIR"
BASE COUNT         1886 a         1181 c          935 g         1697 t
ORIGIN      
        1 catcattttc tccttacaat cttttttctt atttatacca taaaaataaa tttatcgctc
       61 gttcttcaag cgataaaaca agcgataaac tattacaatt tattttaaat tctttaacga
      121 ttactaactt tttaacgtta ttattttcaa atactctaca taaaattacc ctagagtatt
      181 aaaactccta gtccctttgt ttcaagctat ttagaacttt tattgtatta tccactttat
      241 tcctatttct gttgctatta gtttaaccat gaaatctaac ctttaccctt tttagggagt
      301 gcaaaatcca aatgaatcgt gcatctcaac tcccaatata acttctttca ttataccaca
      361 gataaactaa aaaaggacgt gccgaattga tacgcccttg ccttttatca atcaatccgt
      421 acgccatttt tgaaggttag gctgatgtgc ttgtctactt ttatatccaa cctttcaaca
      481 cttgtctgcc agagttctcc ctttggacca ggagttaaag agatggttgt aaggtcagct
      541 tgtgtcgcta gactttcccc tctcaccttt aggagcggtg tgtcaatgga aagattccca
      601 tccctatccc aactaaagac tggagtattc tgtcctggaa taacgacctt atcagcttca
      661 acttctaggc tcttcacata ctctgataga atctgttggg agataagcct tgtcatccat
      721 gctgaactag agacggtcaa ctcatcaatc ttagcttttt gaagtgctgc aactcgtgct
      781 tctaaggcat ccgttgccag ataatcaaga gcagctttct tatcagaacg ctcgccttct
      841 gctttagcag tagcgatacc ataccacttt tatctcgtat aatctgcaca taggcatttc
      901 cccatatcaa aagatggctc atgattgcct ctcgaaagat aaaggaggac atctctggat
      961 tgggttcatc atgaagaaga aagtacagtg ggtggtccag ctttttctcc ttcccatttt
     1021 ctgtccgttc atagatgtga ataggaaatg acgccaccgc ttcagcaaga atacgcacac
     1081 aggcatagac tgccgtcgtg tgcatagtct taaactcatc cacattctcg ccactagtcg
     1141 tgcgaccaaa gaggtaggaa aagtcctgac cttcataact gttttgaggc ttatctctag
     1201 cccttttttt ctaattaaat caagtagtcc cataagccct ccttattttg gggtacaaaa
     1261 aaagcacctc ctttgaagtg ctttcggtat atttctttca aagtaatcaa cagtctttct
     1321 tacaaaacct agtattctca ttctgtcaat tccttaaatg cgtcaagatg tcgtgataat
     1381 actgaagtcg caatctcatc taaccttgct tgttcaacaa ctgttgtcat ctgctcatca
     1441 ccaaagttcc atcagttgat ttataaaggt taaactcctg attctcttga atgccaaata
     1501 ggtcaggtat cggaggatag atgtcatcac caatttgtat cgtctttacg agttccatac
     1561 atcactctcc cttactttac caattattca tatgacgtga tccatagacg accgcaacaa
     1621 ttataacttc atcttccaga acatgatata caatgcgata ttttttgaca atcagttgtc
     1681 tgaaggtata gccttttcca atcagatcct caatgatggc acaacgttca ggaaaaatgg
     1741 atagtgataa cattccttgg gataacctct caagaagatt atccgctgcc tgtggtgcac
     1801 agagttcatc acgaacatag tgatagatgc tcagcaaatc tgctttagca tcatccgaca
     1861 tggtgacctg atattctttc attaagcatt ttccttcaat tgaaatagaa attctctgac
     1921 atcctgacgg ttgccagttt tcgcatcttc aaagctggtg ataagcttat cataaaactt
     1981 ctcctgactc atgtaatcaa cattaactcg ttgaggcgct tcaggtagag aaacatcaaa
     2041 tgggatgccc ccagtcaaaa taatctgatt caaaaacata tcaatcgcag ttgacatagg
     2101 aatacctaag cgtttcaaaa tatcatctgc cgcacttttt actgaatcat caactcgtaa
     2161 attcaaagtt cccgttttag ccatggttat cctccttttt tgtaacgaca ttgtatcaca
     2221 tttttggagt tgactcaact aaaaactcaa tatccctcgc tcatcataga cgctagcttc
     2281 atcaccttgg cggcgaatgc aacggtcaag ccccaggata agacttacaa taccgtcaat
     2341 cttctcaaca gatttttcct tatctggctt gatgttgcct gcaggagctt gtcgcatcac
     2401 cacgttttgt cccatccatt taacgactgg atgaccacca tgttagattt tcccttccat
     2461 catgagttta tagagtccct tggacggtgg actcatatcc ttataaccct gcccaaaagg
     2521 caccatggtc aagtcgacac cgcaattcca aggtatcttc tggtaaccat cagagagaag
     2581 acagggattg taaagttaac cactgtgcaa aatacagcgt tcactaccca gtcgaatcaa
     2641 ctcaaaaaac aatacgggca gttcttgtta acagaatcta ataactgttg tgcatgccca
     2701 ggctgtgata gaccactcat tctaaccagt ggcaacttag cagcagaaaa ttacgaggtt
     2761 tcagccattg aaaaagataa ggacgctgaa ccactgaatc tgatagccct ctgcccagat
     2821 tgttttttaa cctatcaagc agatagtcgc aaaaaaattg taacagctct aaaaaatgtt
     2881 aaaaagattc tagtctctgc acataacagc caacaagcga tttctgatat gaagttagac
     2941 agtggaattg tttcagtttt gactagtctc aataaactaa aatgtgatga atatgatatc
     3001 tcctatgact cgaagcaatt gactgataag atatcacctg agaacaatcg tacgctttat
     3061 caattggtta aaaacccagt tattgataac tacttaacca ttcaaaaaat catcgtaaat
     3121 ttggacaaac aaggaaaaat tgattacgag gaaatccaat atcaaacgcg atcgatgtat
     3181 aaaaagttaa aagcagccaa gcataataat ctggctattt tcaatactat ttcggaaaaa
     3241 ctccataaag ctacactaca ggacatctac ttctgtcaga tgattgtctc ttactttatc
     3301 caaaaatgcg aggtacttga ataatgcaat taccaaataa actttactcc tatcaaaaaa
     3361 gcaccctggc ctatttacct agagtgctaa atgaaatcaa aagtggaaac tcaaacgtca
     3421 aagatatctt tcacgctatc tcagaagagt tggatgatcc tacagatttt ctatccataa
     3481 tggattgctt gtatgcattg aacgctattg aaatgtctaa tgaaggagaa gtaaaattat
     3541 gcttatagaa atgtggtctc cagtatttaa gaaacacgga gaaattcggg aaccaatctg
     3601 cttccaccat gggttaaacg tgattatggg gatggattta gctgataact ctatcggcaa
     3661 atcctcctca ctattagcca ttgatttcat ctttggaggt aatagctatc taaaatcaat
     3721 tgcggttaaa aaattaggtg atcatcctat ctacttctgc tttcaatttg agaaaaagtt
     3781 ttatttttca agagatgcgg ccaacccaga tatcattcta gtgtgtgaca agagctactc
     3841 tccaaccggt gaaacaatgg acctaggaat ttttcttaac aaacttaaga aacgatatca
     3901 tttagattct ccagagctat cattccgatt agctatgagt ggcttcttta ggattgcagg
     3961 aaaaagtaat caaaataccg atttcccctt acaagtttat tcgagccaaa aatcttctga
     4021 atctattact actctcatcc aactgtttaa tttgtatgat aacattgcac gatacaaaga
     4081 acgtctgaaa gataagagcg accagttaac aacttttcgt aatgctcgaa agtatgcctt
     4141 catctccaac ctggttggcg gtaaaaagca atttgaagcg aatgtctcag aaataaaaag
     4201 attggaatat gatatagcac atctccaaga tactcatcaa gataaaattg actctgatga
     4261 tattgagaaa aatcaacaaa aactccaact tagaaatact aagctggagt tagaaagcag
     4321 tcttcgggac aagcaaagac gtcttaagct attggacatc agtatcgaat ttggcttata
     4381 tcctactgaa tctgacttaa cagagcttca aaaatacttc ccagatacta atctcaaaaa
     4441 attatatgaa gttgaagctt accacaaaaa attggcaact attttagatt ccgaattctc
     4501 aactgaacgt gaatccctaa tagctgagat cgatgaatta gagagtcaat taacaattct
     4561 aaaccaagaa cttcaagaac taggaaacat cccaaatctt tcaagtgagt acttagaaaa
     4621 ctactctaaa ctaacagcta caatcaatgc actaaaagaa caaaacgagg cctacttaaa
     4681 ggagtctggt ctatccaaag aaaaatcaga agcagacgct gacttgaaac gcagtacaga
     4741 agatatcctt ctagatctag aagtaaaaat caacagtaag atgcgtgaat tcaataacat
     4801 tctatatcca gatattcgca aagctcctca attaacctca atgctcacaa tagctactcc
     4861 ttctatacac cagatgatga tggtacagga actaagttca aagggatgat tgtattcgac
     4921 ttagtaatgc tctaccttac taatctgcca gcactcgcac acgattcatt actcttgagt
     4981 aatgttagct accaagcgac tgaagcactt ttgaagctgt atgaccagtc aaagtcattg
     5041 aacaaacaag tctttttagc cttcgataaa gccagctctt attctccaga tgctaatcaa
     5101 ctcttgtcag aaaatacggt attgagactc tctagtaacg gaaatgaact ctacggtatt
     5161 tcatggaaca aaggagaaaa ctcagatgag atttagttac aataaattat ggaaacttct
     5221 tattgataaa ggttggacca aaacaaagct caggcaagaa gctgggatta gttcttccac
     5281 gatcgcaaaa ttgggaaaag gagaaaatat cactactgat attttattgg aaatttgtat
     5341 tgcacttgat tgtaagattg aagatatagt agaaattgta gataataata tctagaaata
     5401 aaaaaagcac tgagttgata agatgtgtaa gtcagatcaa cttagtgcct ttactttgaa
     5461 aaaaataatt ttctcaataa tacttagtac taactgttaa cgaatattat actttttaaa
     5521 taatattcta aatactaatg acaatatagt aatgcacgaa ccaataataa aaagattaaa
     5581 gctattgtta ggatttaatg ccaccgagaa aaagccagtc ccaataggca taaaaagaat
     5641 agttattgta aagataatac caaacactct ccctaaaaaa tcgttatcaa catctttct
//
LOCUS       DAUCWO010000078         5438 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUCWO010000078 DAUCWO010000000
VERSION     DAUCWO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.078"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            78..2348
                     /locus_tag="VGY20_001555"
     CDS             78..2348
                     /locus_tag="VGY20_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ4274383.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMFLTLVSSMRGA
                     QSIFGEEKRIEEVSVPKIKSPDDAYPWYGYDSYDSSHPYYERFKVAHDLRVNLNGSKS
                     YQVYCFNINSHYPNRKNAFSKQWFKRVDGTGDVFTNYAQTPKIRGESLNNKLLSIMYN
                     AYPKNANGYMDKIEPLNAILVTQQAVWYYSDSSYGNIKTLWTSELKDGKIDFEQVKLM
                     REAYSKLISDDLEETSKNKLPQGSKLNIFVPQDKSVQNLLSAEYVPESPPAPGQSPEP
                     PVQTKKTSVIIRKYAEGDYSKLLEGATLRLTGEDILDFQEKVFQSNGTGEKIELSNGT
                     YTLTETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVAEPYSVEAYSDMQD
                     SNYINPETFTPYGKFYYAKNKDKSSQVVYCFNADLHSPPESEDGGGTIDPDISTMKEV
                     KYTHTAGSDLFKYALRPRDTNPEDFLKHIKKVIEKGYNKKGDSYNGLTETQFRAATQL
                     AIYYFTDSTDLKTLKTYNNGKGYHGFESMDEKTLAVTKELINYAQDNSAPQLTNLDFF
                     VPNNSKYQSLIGTEYHPDDLVDVIRMEDKKQEVIPVTHSLTVKKTVVGELGDKTKGFQ
                     FELELKDKTGQPIVNTLKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETE
                     AKDYIVTVDNKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVP
                     FGLLVWFFGRKGLKND"
     gene            2341..2862
                     /gene="lepB"
                     /locus_tag="VGY20_001556"
     CDS             2341..2862
                     /gene="lepB"
                     /locus_tag="VGY20_001556"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4274384.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            2884..3927
                     /locus_tag="VGY20_001557"
     CDS             2884..3927
                     /locus_tag="VGY20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ4274385.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVTGKSLQVTKT
                     MTYDDEEVLMPETAFTFTIEPDMTASGKEGDLDIKNGIVEGLDKQVTVKYKNTDKTSQ
                     KTKIAQFDFSKVKFPAIGVYRYMVSEKNDKKDGIRYDDKKWTVDVYVGNKANNEEGFE
                     VLYIVSKEGTSSTKKPIEFTNSIKTTSLKIEKQITGNAGDRKKSFNFTLTLQPSEYYK
                     TGSVVKIEQDGSKKDVTIGTPYKFTLGHGKSVMLSKLPIGINYYLSEDEANKDGYTTT
                     ATLKEQGKEKSSDFTLSTQNQKTDESADEIVVTNKRDTQVPTGVVGTLAPFAVLSIVA
                     IGGVIYITKRKKA"
     gene            3943..4668
                     /gene="srtB"
                     /locus_tag="VGY20_001558"
     CDS             3943..4668
                     /gene="srtB"
                     /locus_tag="VGY20_001558"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ4274386.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            4685..5272
                     /locus_tag="VGY20_001559"
     CDS             4685..5272
                     /locus_tag="VGY20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ4274387.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
BASE COUNT         1947 a          862 c         1038 g         1591 t
ORIGIN      
        1 atatagtagc tatgaaatag cattgtgact gtccggtaaa gggatgggac tgaagatttt
       61 attcagaagg agagggaatg aataacaaaa aattgcaaaa gaagcaagat gctcctcggg
      121 tatcaaacag aaagccaaaa caattaactg tcactttagt gggagtattt ttaatgtttt
      181 tgaccttggt aagttccatg agaggtgctc aaagcatatt tggagaggaa aagagaattg
      241 aagaagtcag tgttcctaaa ataaaaagtc cagatgatgc ctacccttgg tatggctatg
      301 attcatatga ctctagtcat ccttactatg aacgttttaa agtagcacat gatttaaggg
      361 ttaatttaaa tggaagtaag agctaccaag tatattgctt taatatcaat tctcattatc
      421 cgaatagaaa aaatgctttt tctaaacaat ggtttaagag agttgatggg acaggtgatg
      481 tgttcacaaa ttatgctcag acacctaaga ttcgtggaga atcattgaat aataaacttt
      541 taagtattat gtacaacgct tatcctaaaa atgctaatgg ctatatggat aagatagaac
      601 cattaaatgc tattttagta actcaacaag ctgtttggta ctattctgac agttcttatg
      661 gtaatataaa aacgttatgg acatctgagc ttaaagacgg aaaaatagat tttgaacaag
      721 taaaattaat gcgtgaagct tactcaaaac taattagtga tgatttagaa gaaacatcta
      781 aaaataagct acctcaagga tctaaactga atatttttgt tccgcaagat aaatctgttc
      841 aaaatttatt aagtgcagag tacgtgcctg aatcccctcc ggcaccaggt cagtctccag
      901 aaccgccagt gcaaacaaaa aaaacatcag tcattatcag aaaatatgcg gaaggtgact
      961 actctaaact tctagaggga gcaactttgc gtttaacagg ggaagatatc ctagattttc
     1021 aagaaaaagt cttccaaagt aatggaacag gagaaaagat tgaattatca aatgggactt
     1081 ataccttaac agaaacatca tctccagatg gatataaaat tgcggagccg attaagttta
     1141 gagtagtgaa taaaaaagta tttatcgtcc aaaaagatgg ttctcaagtg gaaaatccaa
     1201 acaaagaagt agcagagcca tactcagtgg aagcgtacag cgatatgcaa gatagtaact
     1261 atattaatcc agaaacgttc actccttatg ggaaatttta ttacgctaaa aataaggata
     1321 aaagttcaca agttgtctac tgttttaatg ctgatttaca ctctccacct gaatcagagg
     1381 atggaggagg aactatagat cctgatatta gtacgatgaa agaagtcaag tacacacata
     1441 cggcaggtag tgatttgttt aaatacgcgc taagaccgag agatacaaat ccagaagact
     1501 tcttaaagca cattaaaaaa gtaattgaaa aaggctacaa taaaaaaggt gatagctata
     1561 atggattaac agaaacacag tttcgcgcgg ctactcagct tgctatctat tactttacag
     1621 acagcactga cttaaaaacc ttaaaaactt ataacaatgg gaaaggttac catggatttg
     1681 aatctatgga tgaaaaaacc ctagctgtaa caaaagaatt aattaattac gctcaagata
     1741 atagtgcccc tcaactaaca aatcttgatt tcttcgtacc taataatagc aaataccaat
     1801 ctcttattgg gacagaatac catccagatg atttggttga cgtgattcgt atggaagata
     1861 aaaagcaaga agttattcca gtaactcaca gtttgacagt gaaaaaaaca gtagtcggtg
     1921 agttgggaga taaaactaaa ggcttccaat ttgaacttga gttgaaagat aaaactggac
     1981 agcctattgt taacactcta aaaactaata atcaagattt agtagctaaa gatgggaaat
     2041 attcatttaa tctaaagcat ggtgacacca taagaataga aggattaccg acgggatatt
     2101 cttatactct gaaagagact gaagctaagg attatatagt aaccgttgat aacaaagtta
     2161 gtcaagaagc tcaatcagca agtgagaatg tcacagcaga caaagaagtc acttttgaaa
     2221 accgtaaaga tcttgtccca ccaactggtt ttactactga tggtggaacc tatctgtggt
     2281 tattattgct tgtcccattt ggtttgttag tgtggttctt tggtcgtaaa ggactaaaaa
     2341 atgactaatt acctaaatcg cttaaatgag aatccactat ttaaagcttt catacggtta
     2401 gtacttaaga tttctattat tggatttcta ggttacattc tatttcagta tgtttttggc
     2461 gtcatgattg ttaacacaaa tcagatgagt cctgctgtaa gtgctggtga tggagtctta
     2521 tattatcgtt tgactgatcg ctatcatatt aatgatgtgg tggtctatga ggttgataac
     2581 actttgaaag ttggtcgaat tgccgctcaa gctggcgatg aggttaattt tacgcaagaa
     2641 ggaggactgt tgattaatgg gcatccacca gaaaaagagg tcccttacct gacgtatcct
     2701 cactcaagtg gcccaaattt tccctataaa gttcctacgg gtacgtattt catattgaat
     2761 gattatcgtg aagaacgttt ggacagtcgt tattatgggg cgttacccat caatcaaatc
     2821 aaagggaaaa tctcaactct attaagagtg agaggaattt aaaaaaattt ggagagagag
     2881 aaaatgaaaa aaaataaatt attacttgct actgcaatct tagcaactgc tttaggaaca
     2941 gcttctttaa atcaaaacgt aaaagctgag acggcaggag ttgtaacagg aaaatcacta
     3001 caagttacaa agacaatgac ttatgatgat gaagaggtgt taatgcccga aaccgccttt
     3061 acttttacta tagagcctga tatgactgca agtggaaaag aaggcgacct agatattaaa
     3121 aatggaattg tagaaggctt agacaaacaa gtaacagtaa aatataagaa tacagataaa
     3181 acatctcaaa aaactaaaat agcacaattt gatttttcta aggttaaatt tccagctata
     3241 ggtgtttacc gctatatggt ttcagagaaa aacgataaaa aagacggaat taggtacgat
     3301 gataaaaagt ggactgtaga tgtttatgtt gggaataagg ccaataacga agaaggtttc
     3361 gaagttctat atattgtatc aaaagaaggt acttctagta ctaaaaaacc aattgaattt
     3421 acaaactcta ttaaaactac ttccttaaaa attgaaaaac aaataactgg caatgcagga
     3481 gatcgtaaaa aatcattcaa cttcacatta acattacaac caagtgaata ttataaaacc
     3541 ggatcagttg tgaaaatcga acaggatgga agtaaaaaag atgtgacgat aggaacgcct
     3601 tacaaattta ctttgggaca cggtaagagt gtcatgttat cgaaattacc aattggtatc
     3661 aattactatc ttagtgaaga cgaagcgaat aaagacggtt acactacaac ggcaacatta
     3721 aaagaacaag gcaaagaaaa gagttccgat ttcactttga gtactcaaaa ccagaaaaca
     3781 gacgaatctg ctgacgaaat cgttgtcaca aataagcgtg acactcaagt tccaactggt
     3841 gttgtaggga cccttgctcc atttgcagtt cttagcattg tggctattgg tggagttatc
     3901 tatattacaa aacgtaaaaa agcttaagtg attagtcaaa gaatgatgat gacaattgta
     3961 caggttatca ataaagccat tgatactctc attcttatct tttgtttagt cgtactattt
     4021 ttagctggtt ttggtttgtg ggattcttat catctctatc aacaagcaga cgcttctaat
     4081 ttcaaaaaat ttaaaacagc tcaacaacag cctaaatttg aagacttgtt agctttgaat
     4141 gaggatgtca ttggttggtt aaatatccca gggactcata ttgattatcc tctagttcag
     4201 ggaaaaacga atttagagta tattaataaa gcagttgatg gcagtgttgc catgtctggt
     4261 agtttatttt tagatacacg gaatcataat gattttacgg acgattactc tctgatttat
     4321 ggccatcata tggcaggtaa tgccatgttt ggcgaaattc caaaattttt aaaaaaggat
     4381 tttttcaaca aacataataa agctatcatt gaaacaaaag agagaaaaaa actaaccgtc
     4441 actatttttg cttgtctcaa gacagatgcc tttgaccagt tagtttttaa tcctaatgct
     4501 attaccaatc aagaccaaca aaggcagctt gttgattata tcagtaaaag atcaaaacaa
     4561 tttaaacctg ttaaattgaa gcatcataca aagttcgttg ctttttcaac gtgtgaaaat
     4621 ttttctactg acaatcgtgt tatcgttgtc ggtactattc aagaataacg aaaggaggag
     4681 acttttgaga aaatattgga aaatgttatt ttctgtcgta atgatattaa ccatgctggc
     4741 ctttaatcag actgttttag caaaagacag cactgttcaa actagcatta gtgtcgaaaa
     4801 tgtcttagag agagcaggcg atagtacccc attttcgatt gcattagaat caattgatgc
     4861 gatgaaaaca atagaagaaa taacaattgc tggttctgga aaagcgagct tttcccctct
     4921 gaccttcaca acagttgggc aatatactta tcgtgtttat cagaagcctt cacaaaataa
     4981 agattatcaa gcagatacta ctgtatttga cgttcttgtc tatgtgacct atgatgaaga
     5041 tgggactcta gtcgcaaaag ttatttctcg aagggctgga gacgaagaaa aatcagcgat
     5101 tacttttaag cccaaacggt tagtaaaacc aataccgcct agacaacctg acatccctaa
     5161 aaccccatta ccattagctg gtgaagtaaa aagtttattg ggtatcttaa gtatcgtatt
     5221 actggggtta ctagttcttc tttatgttaa aaaactgaag agtaggctat aaaacccatt
     5281 tctagttgct ctatttttat aatgaaactt attgacatct ccatagtctt gcttgacttt
     5341 ggagatgttt ttctatgctg ataggttttt gtaccatata agataatgat tgatgaaaag
     5401 agtgtcttga attcttaaat attatctcgg tatttagc
//
LOCUS       DAUCWO010000079         5381 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUCWO010000079 DAUCWO010000000
VERSION     DAUCWO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.079"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            135..1472
                     /gene="pepC"
                     /locus_tag="VGY20_001560"
     CDS             135..1472
                     /gene="pepC"
                     /locus_tag="VGY20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ4274388.1"
                     /translation="MSALTETFTEQLFAHYEASAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(1937..4102)
                     /locus_tag="VGY20_001561"
     CDS             complement(1937..4102)
                     /locus_tag="VGY20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4274389.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLRSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(4089..4697)
                     /gene="recU"
                     /locus_tag="VGY20_001562"
     CDS             complement(4089..4697)
                     /gene="recU"
                     /locus_tag="VGY20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ4274390.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            4778..5293
                     /locus_tag="VGY20_001563"
     CDS             4778..5293
                     /locus_tag="VGY20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ4274391.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDKWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPPQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPYYQLLLL
                     TFDRLNDMSQS"
BASE COUNT         1649 a          900 c         1080 g         1752 t
ORIGIN      
        1 ctaacttttt tcttgaagaa aatcaaataa ggtctataat agaagtaatc ctttagaata
       61 gaatggctgg ataataacca tcttgccaat attatttcag gtgaagctta tcttaatatt
      121 ttaggaggac aataatgtca gcattaacag agacatttac agaacaatta tttgctcatt
      181 acgaagccag tgcaaaattt tcagctattg agaatgccgt tacgcataat ggtttgctaa
      241 aatcattaga aacccgtcaa agtgaagtgg acaatgattt tgtgttttct attgatttaa
      301 ctaaagataa ggtatctaac caaaaggctt ctggtcgttg ctggatgttt gctgccttga
      361 acactttccg acacaaatta atcacagagt ttaaattaga aaattttgaa ctgtcacaag
      421 ctcatacttt cttttgggat aagtatgaaa aagctaactg gtttatggag caggtcattg
      481 ctacagctga ccaagaatta accagtcgta aagttaagtt tctcttggat gttccacaac
      541 aagatggtgg tcagtgggac atggtagttt ccttgtttga gaagtatgga gtggtgccaa
      601 aatctgttta cccagaatca atctcatcaa gtaacagtcg tgaattaaac cagtatttga
      661 ataaattatt gcgccaagat gctcaaattc ttcgtgacct gattgcgtca ggtgctaagg
      721 cagaccaagt tgaggatcga aaagcagagc ttcttcagga aatttttaac ttcttggcca
      781 tgacccttgg tttaccacct cgtcattttg attttgccta ccgtgataaa gatgaccact
      841 atcatgttga gaaaggtttg acaccgcaag cgttttacga caaatttgtt ggcttaaagc
      901 tttctgatta tgtgtctgtt attaatgcgc caactgctga taagccttat ggcaaatcat
      961 acactgttga gatgctagga aatgtggtcg gtagtcgtga ggttcgctac ctcaaccttg
     1021 atatgaaacg tttcaaagaa ctagctatca agcagatgca ggctggtgag tccgtttggt
     1081 ttggctctga tgtgggtcaa gtttcagatc gtcaaaaagg gattttggca actaatactt
     1141 acgattttga agctagtatg gatatcaatc ttagtcaaga taaggctgga cgtttggatt
     1201 atagtgaaag tttgatgaca catgctatgg ttttaacagg tgttgactta gatgaaacag
     1261 gaaaaccgtt aaaatggaag gttgaaaatt catggggtga gaaggttggt gacaaaggtt
     1321 actttgttgc ttcagatgct tggatggatg aatataccta tcaaattgtt gtccgtaaag
     1381 aattcttaac agctgacgag ttagcggctt atgaaaaaga gccacaagta ctagctcctt
     1441 gggatccaat gggtgcttta gcacaatgct aattcctttc tttgcgactg tatgaaatct
     1501 aaaggctgga caaaacgaac agcaaacgga ttggactccg cataagaaag tgaacatgaa
     1561 aaaaattatt atgctaagaa aatgacatat gaaaaaacac gtcttcggta tatggaaacg
     1621 tgttttttca tgtttactct cttatggcat taggttgcta tttataacaa ttttaaataa
     1681 gtctattttg ttttaggtta acgtttgaaa aatcagttgc taatcccctt aacgttgtac
     1741 cttttcttta atacgattaa agaattctgt tttttgtttt atattgtatg tggtaagtca
     1801 tttgtgtcat ataaaaacga gtcctcacag aacgtgaact cgctttttat ttagattatc
     1861 acttaaggta gatattaaaa gtaactaaat ggatatgtaa taaaaggtag gtacagacaa
     1921 taagtagagt tagagattaa tgagttgtct ttttatcatc ggtagatgga tgattgctat
     1981 tgttgtctgt attggttgaa ttacttatat cattgctact actagtatca tctgaagtcg
     2041 tttggctaga agctgttgtc gtgttattgc tgtaaagatt gttgtaaacc gaattagtat
     2101 agtcggtgtt gctcgcatag gttccgctta agtagaggaa tccaccactg cgataaagac
     2161 catttggcat cgtccaatct tcactgtaac catttgttaa gtaagtcatc atgctacgat
     2221 aaacgtcaga tgcaatctct agacttgatc cgtatactgg ggtcaagcgg tttttgtaac
     2281 ctgtccaaac agccatggcg tagcgcttag taaagccaac aaagttttcg tctggcgcta
     2341 atgtaccaac ataatctgga taaaggccgt atttttcacc aattttagct agttcctcat
     2401 cagtgtagtt agaagtccct gttttaccag cttgcgcaac accaggaatg gcagcagcag
     2461 tacctgtacc ataagtgaga acagttttta acatatctgt catcatatag gccgtggttt
     2521 ctttcatggc acgttttcct ttttcatcaa aagttttact tgtaccatca ctaaattcca
     2581 ctttattgac ataccttggt ttatgataaa taccaccatt tgcaaaagca gcgtatgcag
     2641 cggccatttt ttcactactt gcaccatatt ttttatctga gctactgtta ttacttgaga
     2701 tagcgtttga gtagtgcatt tcgggatagt taatacctaa actgcttaag aaagatcgag
     2761 catagtcaag tcctgcggct tccagtgctc ggactgctgg gacatttcgc gatagcataa
     2821 tagcagcttg gattgtcatc catccgttat atctaaggtc ccagttaaac aattgggtag
     2881 tggttccagg ccaataatag actgagtcat tagtcgactg agcagtagaa gtataaacac
     2941 cagattcaat agcaggagca taggctgtga ttggcttcat ggtagaaccc cagtcacgat
     3001 cagttaaaac agcttggtta gtcccaaatg aaacattttc atcttgatta cgtccgccaa
     3061 gctgagcaat aacatgacca tttgtcacat caacaatcgt tgaagcaact tggaaatctt
     3121 ggtctggata gtaaacataa tcaccagaat gataaatatt ataaagagtc tgctgcgcat
     3181 cggggataat attggtataa acttttaaac cagcggtaaa aatatcttta ttcgtttctt
     3241 ttttgacttc ttcaataact tgttttagat aattatccat atattttgga taagttgagc
     3301 gctgttggag tgatcgtaga ccttcagcga cgggagttgc gatggcagtt tcatattctg
     3361 ctttcgtcag atgtttttcc atgtacatct gttgcaacac gacgttacgg cgattttgag
     3421 cagcttcagg atgaaggtaa ggatcatatt gactaggagc ttgagggatt ccagccaata
     3481 gggctagttg ggcataagat aaatccttaa gatccttgcc ataataagac ttagcggctg
     3541 tcagcatacc atagttgcca ttacccatat atactttgtt gatgtaaaaa gtcaggattt
     3601 cttgtttagt atattttcgc tccatttgaa gagcaagcca aacttcttga gccttacgtt
     3661 ttaaggtttg atcagattca ttagtagaaa aataggctag tttaatgagt tgctgatcaa
     3721 gcgttgaccc cccttgagtg gtctgactcg ttagattatg aaaggcagca ccaaaaatac
     3781 gataaagatc tactccacga tggttaaaga aacgtttatc ttcaattgag gtaatagcat
     3841 taactagatt aatagggata ctatcagctg ttacattttc acgcttttca gaacccaaat
     3901 cagcaatcag attgttatta ccatcataaa ccaagctaga gtttgttgat tttaactggg
     3961 cttctgacag tttcggagca ctgctaatgt agaaggtaaa caaaagacca ccaataataa
     4021 taacaaggat aataaggcta agaattgcac ttaatacata ctttagccac ttaaggattt
     4081 ttggattttt aattgtaatc accgcctaaa aaattttgtt caattatgtt taaataaggg
     4141 acttgtggaa aggctccaaa tgctacctta aagccatttt ttctgatata atcaatgggc
     4201 atcgatttac tgccattatc tatctgataa aatgaaatca aggcttgagc aggtaggtag
     4261 taagtctcct tcagtgtgga aaagtgaaga aggacaaagc agattccttt ttgatgtaaa
     4321 acgcaagcca tatgttcaat ctgatgtaaa tggaaatttt tcatgggcat agctgttttt
     4381 tgcctggttt cttttgcttc aaaatcaaca taatgtcctt tataaacgcc gcaatagtca
     4441 gtcgttgaag cttgtctaaa gtacgcttca acgatttttg cccgactgcg tttagggtaa
     4501 tccactttga caatttggac aggagtaggt tttttgtgaa tgacagctat ttgacgagat
     4561 aaataataat catttgtcgc gttgatagca gcttcaaagg acatacctct attggcgaag
     4621 tccaccttat tttgctttgt ttgcttttga acggaactta ctttttgtcg aataagatta
     4681 tgaggataat taaccattgt tctcctaata ttatctagtt aatgctaaaa atttaagcac
     4741 ttttattata acatattttt tgaaaggata acacctaatg actgcgattc taattacagg
     4801 ttaccgaagc tttgaaatag ggatttttga ccataaagac ccacgagttt caattattaa
     4861 acaggctatt cgtaaagatt taataggtta tttggaaaat ggtgtggatt ggtttatctt
     4921 tacagggaac ttgggttttg aacaatgggc tttggaagta gctaatgagc taaaagagga
     4981 gtatcccttg cagatagcta ctatattctt gtttgaaacg catggagata agtggaatga
     5041 aaaaaatcaa gaagttttaa gtcagtttag ggctgttgac tttgtaaaat attactttcc
     5101 aaattacgaa caaccgccgc aatttagtca atattatcag tttttattag aaaagacaga
     5161 aggggcttat gttttttatg acacagaaaa cgagaccaac ttaaaatatt ttctcaagaa
     5221 agcaaaagat atgccgtatt atcagttgct attgctaacc tttgatcggc taaatgatat
     5281 gtcacaaagc tagcattgta tttttagggg tattggtatt taaatacttg ctttttgaac
     5341 tatttttctg tatcattaat aatactgaaa tataattttg g
//
LOCUS       DAUCWO010000080         5304 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUCWO010000080 DAUCWO010000000
VERSION     DAUCWO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.080"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            132..1403
                     /locus_tag="VGY20_001564"
     CDS             132..1403
                     /locus_tag="VGY20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ4274392.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNMLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVTVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            1403..2113
                     /locus_tag="VGY20_001565"
     CDS             1403..2113
                     /locus_tag="VGY20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274393.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEIFNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            2125..3345
                     /locus_tag="VGY20_001566"
     CDS             2125..3345
                     /locus_tag="VGY20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4274394.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            3599..>5304
                     /locus_tag="VGY20_001567"
     CDS             3599..>5304
                     /locus_tag="VGY20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEQ4274395.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDISHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SR"
BASE COUNT         1787 a          792 c         1018 g         1707 t
ORIGIN      
        1 ttaatttgtc atagcatata agtcattatt ttgtggcttg ttttcgacta aggactaaga
       61 atttcttgac aacaatataa aaaggtcata tacttgtctt atacaaatag tacaaaaagg
      121 ggaggataga aatgtctaaa agaggaaaaa ttaaaataac aacgaaaaca aagcttatta
      181 cagctagtgt tataacgcta gtattaatta taactggagt agtcttgtgg aaacaacaac
      241 aaaatatgct aacagctgat atcgctaaag aaccttactc tactgttagt gtaactgaag
      301 ggagtattgc ttcttcgact ttactatcag gtactgtaaa ggctttatca gaggaatata
      361 tttattttga tgctaataaa ggaaatgatg caactgttac agttaaagta ggtgatcagg
      421 taacgcaggg ccagcaatta gttcaatata atacaacaac agctcagtca gcttatgata
      481 ctgctgttag gagtcttaac aagattggcc gacaaattaa tcatcttaaa acatacggag
      541 ttcctgctgt tagtacagaa actaataaag atgaagctac cggtgaagag acgacaacaa
      601 cagttcaacc atcagctcag caaaatgcta attataaaca gcagctgcaa gatttaaatg
      661 atgcttatgc agatgcacaa gcagaagtaa ataaagcgca gatagcgtta aatgatacag
      721 tagttatcag tagtgtctct ggaactgttg tggaagtaaa taatgatatt gatccttctt
      781 caaagaacag tcaaacactt gttcacgtag caaccgaagg acagcttcag gtgaaaggaa
      841 cattgacaga gtatgattta gcaaacgtta aggttggtca atctgtaaaa attaagtcta
      901 aagtttattc taatcaagaa tggactggaa aaatatcata tgtttcaaac tatccaactg
      961 agtctaatgc aggttcaaca acgccagcag gtagcactgg agcgggaagt tctacaggag
     1021 ctgcctatga ttacaagatt gatattataa gtcctctcaa ccagcttaaa caaggcttca
     1081 ctgtttctgt tgaggttgtt aatgaagcaa aacaggcctt agttccttta acggctgtta
     1141 ttaagaaaga taaaaaacac tatgtttgga cttatgatga tgctactggc aaagccaaaa
     1201 aagtagaggt gacacttgga aacgcagatg cacaacaaca agaaattcat aaaggagtaa
     1261 ctgttggtga cattgttatt gccaatccag ataaaaatat caaaccggat aaaaaactag
     1321 aaggggttat ttcaataggt accaacacaa aaccggaaaa agattctcaa tcaaagaata
     1381 aaaaatcagg ggtggataaa tagtgtcgca tgaaaaaaaa caattaatgc aattatcaaa
     1441 tattgtgaag agctatcaaa atggggatca agttcttaaa gttttgaaag gtattaattt
     1501 aacagtatat gagggtgaat ttttagctat tatgggtccg tctggctcgg gaaaatcaac
     1561 actaatgaat attattggtt tattagatcg gccaacatcc ggagattaca cattacataa
     1621 tactaaaata gaaatcttta atgatcgtga attagcaaaa gtacgaaatg atgaaatcgg
     1681 ctttgtcttt caacaattct ttctgttagc taaattaact gcattacaaa atgttgagtt
     1741 gcctcttatt tatgctggtg taaacgtctc aaaaagacgt gaacaggcta agcaatttct
     1801 tgaaaaagta ggacttggac gacgtatcaa acatctaccg tcagaactgt caggaggtca
     1861 aaagcaaagg gtggctatcg cacgagcttt agtaaatgac ccatctatta ttttagcgga
     1921 tgaaccaaca ggtgcactag atactaaaac aggtcagcaa attatggaac tcttgacaga
     1981 gttaaacaaa gaaggtaaaa caattattat ggttactcat gaaccagaaa ttgctgattt
     2041 tgcaactcgg aaaattatta ttcgtgatgg tgatatcaca acggatacta cagctagtgt
     2101 tgttattgac taggaggaaa aataatggaa aattggaaat tcgctcttag ctcaatttgg
     2161 gggcataaga tgcgctcaat cctgacaatg ttgggaatta tcatcggtgt agcagcagtt
     2221 gtgattatta tggggttggg aaatgctatg aaaaatagtg taacaagcac attttctagc
     2281 aagcaaaaag atattcaact ctattttcaa gaaaaaggag aagaagaaga cctgtatgcc
     2341 ggattgcaca cgcatgaaaa taatcacgaa gtgaagccag agtggttaga acaaattgtt
     2401 aaagatatcg atggtattga ttcttattac tttaccaatt ctgctacttc tactatttcg
     2461 tatgaaaaaa agaaagtaga caatgcttcg atcatagggg taagtaaaga ttattttaat
     2521 ataaaaaact atgatatcgt agctggtcgt acactaacag ataatgatta ttctaatttt
     2581 tcgcgtatca ttcttttaga tacagtatta gccgatgacc tatttggcga aggtaattat
     2641 aagtcagctt taaataaagt ggtaagttta tctgataaag attatttagt tattggtgtt
     2701 tataaaactg atcagacgcc agtttctttt gatggattat caggcggcgc tgtcatggca
     2761 aatactcagg tagcttcaga gtttggcacc aaagaaatag gatctatcta cattcatgtg
     2821 aacgatattc aaaacagtat gaacttagga aatcaagcag ctgatatgct aaccaacata
     2881 tcgcatatta aagatggaca gtacgctgtt cctgataata gcaaaatcgt tgaagaaatc
     2941 aatagtcagt tcagtatcat gacaacagtc ataggttcaa ttgcagcgat ctctctttta
     3001 gtgggaggaa ttggtgttat gaatattatg ttggtatctg tcacagagcg aactagggaa
     3061 attggtttac gtaaagcact tggtgcaaca cgcctgaaaa ttttatcaca atttttgatt
     3121 gaatccgttg ttctaacagt tcttggaggc ttgataggat tattattagc tcaattaagt
     3181 gtaggggctt taggaaatgc gatgacatta aaaggagctt gtatttctct tgatgtggcc
     3241 ttaatagcag ttttattttc tgcctcaata ggtgtctttt ttggaatgtt gccagctaat
     3301 aaagctagta agttagatcc aattgaggca ttacgttatg aatagaatag ttcgtatcaa
     3361 ttaatctata gacgctgact agtttaacgt ctgattatat ctattttttg tattagcaca
     3421 gtagttatat cattgaagtg ttagtgcatt ttttgaggtg ataatatcta gcaaagctat
     3481 cattggtatg atgatgtgga tagcgtataa gtgatacatc tcttatttct agctgaactt
     3541 cggttcggct aggaagttgt caggcaatga gttgtttaaa ataaaggaga ttagttcaat
     3601 gacattttta tctgatttga tatcattaat gacaaaaatc agattatcct gggtaataaa
     3661 ggcgggtatt tttcaattat tatttgtaac gattgctaat attgtcttat cagaattttt
     3721 ctattttata ttagacgtta ctggtcaata tcatttagat aaagacaatg ttgtgacttt
     3781 tttaaaaaat cctatagcac ttgctttatt aggtgcctat ttatttttat tagctgcttt
     3841 tattcacctt gagttttttg ctctatatcg aattattgcg gatcaagaaa ttagtttcta
     3901 tctttttaga aaacagtttt cttattacct aagggggctt tggaaaacat tttctggtta
     3961 ccaattatta ctttttttgc tttatatcct actgactatt ccagtcttac atattggttt
     4021 atcttctgtg attactcaaa agctttatct tccagaattt attgttgggg aattatcaaa
     4081 gataactagc acaaagtact tgctttatgg cagtcttatt cttgtgtttt accttaacct
     4141 aagattagta tattttttac cattgatagc aatcaaccat cgtacggttg ctcaagcatg
     4201 gagagagagt tggcaaaaga ctaaaaagaa acatgtattg ttatggatga aactttttgc
     4261 aatcaatggt cttacgcttg tagtcttatc gctagctatt tccatgattc ttatttttgt
     4321 tgatatgttt aatcctaagg ggaataatat tattgttcag ctgggagctt tgacctttac
     4381 atgggagctc atttttttta ctactatttt ttttaaactc tgttcagcaa tgattttaaa
     4441 agaggcaatt gaaccacaaa agcaatatga tgagccaaga agaagtaata aggcatatgt
     4501 tgtaatcttt atcgtggtga cagtaggttt tgcttatcaa tctcttgaac gtttaacttt
     4561 ttttgacata tctcactcta agacagttat cgcgcataga ggacttgtat cagcaggtgt
     4621 agaaaattct ctggaagccc ttgaaggtgc taagaaagca ggaagtgatt atgtagaact
     4681 ggatctaatc ttgactaagg ataatcactt tgtggtgtct catgataatc gattgaagcg
     4741 tttagctgga gtaaataaga cgattcgcaa cttaacctta aaagaagttg aacatctaac
     4801 gagtcatcaa ggacattttt cagggcgttt tgtttctttt gacacttttt atcaaaaggc
     4861 taagaagttg aatatgccat tacttattga actcaagcca attggtacag aacctggaaa
     4921 ttatgtcgat ttgtttttag aaacttatca tcgacttggt ataagcaaag ataataaagt
     4981 catgtcttta gatttagaag taatagaagc tatcaagaaa aaaaatccat caattacgac
     5041 tggttatatc ataccaattc aatttggctt ttttggagat gaatttgttg atttctatgt
     5101 cattgaagac ttttcttatc ggtcttattt gtcgtcccaa gctttttgga ataataaaga
     5161 aatttacgtt tggactatta atgatcccaa gcgcatagag cattatctcc taaagcctat
     5221 tcagggaatt attacagatc aaccagcttt aactaatcaa ttgattaaag acttaaaaca
     5281 agataattct tattttagtc gatt
//
LOCUS       DAUCWO010000081         5063 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUCWO010000081 DAUCWO010000000
VERSION     DAUCWO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.081"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            553..3579
                     /locus_tag="VGY20_001568"
     CDS             553..3579
                     /locus_tag="VGY20_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ4274396.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQINSGSQLEQSKESLSLNKRVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDEAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGSLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLIQLTSIRQVPT
                     AVYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSHPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLIAAIKKKKY"
     gene            3599..4000
                     /locus_tag="VGY20_001569"
     CDS             3599..4000
                     /locus_tag="VGY20_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ4274397.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(4180..5055)
                     /locus_tag="VGY20_001570"
     CDS             complement(4180..5055)
                     /locus_tag="VGY20_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ4274398.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSSIKVN"
BASE COUNT         1796 a          782 c          963 g         1522 t
ORIGIN      
        1 acaaattatc ctaacatgat gtattgaaag gagaaatagt ttatgttagt atactgatta
       61 agtgaagctg taatgaggct tatacgatca aaaaagttag agtaacttta taaaacagtg
      121 gtgttgaagc aagtttagct tcttctcaga aatagattat ctataaaaaa tcccaaaagt
      181 agtttgtttc cagttaatga acttcttttg ggaaggcttt tttgctgatc gccacttcac
      241 ttgtatatcg ggaacattca cgctaagatg tataagttga atcgtttgtc tatggcttga
      301 tgaaaattaa tcaaaagata atgttcaact ctataattag tacaagcaaa atgagataac
      361 ttattcatta gtaagcataa gatataacaa gagaaattac cttaaaacag gaagtactag
      421 agtatttaaa ataagtgata gacttggata ttattaggtc gactagtgtc cttttcaata
      481 atttaatatc gttattatta gtatcagcca atcaaatgtg gataagttgg cttttagaaa
      541 ggttttacca caatgaagaa acatcttaaa acagttgcct tgaccctcac tacagtatcg
      601 gtagtcaccc acaatcagga agtttttagt ttagtcaaag agccgattct taaacaaact
      661 caagcttctt catcgatttc tggcgctgac tacgcagaaa gtagcggtaa aagcaagtta
      721 aagattaatg aaacttctgg ccctgttgat gatacagtca ctgacttatt ttcggataaa
      781 cgtactactc ctgaaaaaat aaaagataat cttgctaaag gtccgagaga acaagagtta
      841 aaggcagtaa cagagaatac agaatcagaa aagcagatca attctggatc tcaactagaa
      901 caatcaaaag agtctctttc tttaaataaa agagtgccat caacgtctaa ttgggagatt
      961 tgtgatttta ttactaaggg gaataccctt gttggtcttt caaaatcagg tgttgaaaag
     1021 ttatctcaaa ctgatcatct cgtattgcct agtcaagcag cagatggaac tcaattgata
     1081 caagtagcta gctttgcttt tactccagat aaaaagacgg caattgcaga atataccagt
     1141 agggctggag aaaatgggga aataagccaa ctagatgtgg atggaaaaga aattattaac
     1201 gaaggtgagg tttttaattc ttatctacta aagaaggtaa caatcccaac tggttataaa
     1261 catattggtc aagatgcttt tgtggacaat aagaatattg ctgaggttaa tcttcctgaa
     1321 agcctcgaga ctatttctga ctatgctttt gctcacctag ctttgaaaca gatcgatttg
     1381 ccagataatt taaaagcgat tggagaatta gctttttttg ataatcaaat tacaggtaaa
     1441 ctttctttgc cacgtcagtt aatgcgatta gcagaacgtg cttttaaatc aaaccatatc
     1501 aaaacaattg agtttagagg aaatagtcta aaagtgatag gggaagctag ttttcaagat
     1561 aatgatctga gtcaactaat gctacctgac ggtcttgaaa aaatagaatc agaagctttt
     1621 acaggaaatc caggagatga tcactacaat aaccgtgttg ttttgtggac aaaatctgga
     1681 aaaaatcctt atggtcttgc tactgaaaat acctatgtta atcctgataa gtcactatgg
     1741 caggaaagtc ctgagattga ttatactaaa tggttagagg aagattttac ctatcaaaaa
     1801 aatagtgtta caggtttttc aaataaaggc ttacaaaaag taaaacgtaa taaaaactta
     1861 gaaattccaa aacagcacaa tggtgttact attactgaaa ttggtgataa tgcttttcgc
     1921 aatgttgatt ttcaaaataa aactttacgt aaatatgatt tggaagaagt aaagcttccc
     1981 tcaactattc ggaaaatagg tgcttttgct tttcaatcta ataacttgaa atcttttgaa
     2041 gcaagtgacg atttagaaga gattaaagag ggagccttta tgaataatcg tattgaaacc
     2101 ttggaattaa aagataaatt agttactatt ggtgatgcgg ctttccatat taatcatatt
     2161 tatgccattg ttcttccaga atctgtacaa gaaatagggc gttcagcatt tcggcaaaat
     2221 ggtgcaaata atcttatttt tatgggaagt aaggttaaga ccttaggtga gatggcattt
     2281 ttatcaaata gacttgaaca tctggatctt tctgagcaaa aacagttaac agagattcct
     2341 gttcaagcct tttcagacaa tgccttgaaa gaagtattat taccagcatc actgaaaacg
     2401 attcgagaag aagccttcaa aaagaatcat ttaaaacaac tggaagtggc atctgccttg
     2461 tcccatattg cttttaatgc tttagatgat aatgatggtg atgaacaatt tgacaataaa
     2521 gtggttgtta aaacgcatca taattcctac gcactagcag atggtgagca ttttatcgtt
     2581 gatccagata agttatcttc tacaatggta gaccttgaaa agattttaaa actaatcgaa
     2641 ggtttagatt attctacatt acgtcagact actcaaactc agtttagaga catgactact
     2701 gcaggtaaag cgttgttgtc aaaatctaac ctccgacaag gagaaaaaca aaaattcctt
     2761 caagaagcac aatttttcct tggccgcgtt gatttggatg aagccatagc taaagctgag
     2821 aaggctttag tgaccaagaa ggcaacaaag aatggtcagt tgcttgaaag gagtattaac
     2881 aaagcggtat tagcttataa taatagcgct attaaaaaag ctaatgttaa gcgcttggaa
     2941 aaagagttag acttgttaac aggattagtt gagggaaaag gatcattagc gcaagctaca
     3001 atggtacaag gagtttattt attaaagacg cctttgccat tgccagaata ttatatcgga
     3061 ttgaacgttt attttgacaa gtctggaaaa ttgatttatg cacttgatat gagtgatact
     3121 attggcgagg gacaaaaaga cgcttatggt aatcctatat taaatgttga cgaggataat
     3181 gaaggttatc atgccttggc agttgccact ttagctgatt atgaggggct cgacatcaaa
     3241 acaattttaa atagtaagct tattcaatta acatctattc gtcaggtacc gactgcagtc
     3301 tatcatagag ccggtatttt ccaagctatc caaaatgcag cggcagaagc agaacagtta
     3361 ttgcctaaac caggtacgca ctctgagaag tcaagctcaa gtgaatctgc taactctaaa
     3421 gatagaggat tgcaatcaca cccaaaaacg aatagaggac gacactctgc aatattgcct
     3481 aggacagggt caaaaggcag ctttgtctat ggaatcttag gttacactag cgttgcttta
     3541 ctgtcactaa tagctgctat aaaaaagaaa aaatattaaa ttgatttaag gagaaaaaat
     3601 gataacacaa gaaatgaaag atcttattaa taaccagtta gcaatggttg cgacggttga
     3661 tgccaaaggt caacccaata ttggtccaaa acgttctatg cgtctctggg atgataaaac
     3721 atttatttat aatgaaaata cagatggtca aactcgtatt aacattgaag ataatgggaa
     3781 aattgaaatt gcctttgttg atcgtgagcg ccttttaggt tacaggtttg taggaactgc
     3841 tgaaattcaa acagaaggtg cttattacga agcagcgaaa aaatgggcac aaggacgcat
     3901 gggggtacca aaagcagttg gtattattca tgtcgagcgt atttttaatt tacaatctgg
     3961 tgctaatgca ggtaaagaaa taaacagtga aagtaactaa aattaactgt aacagtattt
     4021 aggaccctta ttttaaacgc taaaaaacaa gcatactctt ttttacagca tagtgactat
     4081 gttgtaaaaa ggggtatgct tattaggtgc tcttaatatt tctagataac tgcatctaat
     4141 aagtttttaa tagacttata gcacattaat agacttcaat tagttgactt taatactaga
     4201 aagagaagaa cagtgatgtt ggtgttcgtt taaagaagta tcgatttcca cagtgactga
     4261 gtttatattg tacctttgac aaatcagtct aatagatttt ttgcaatgtt tttcagtggt
     4321 agattctctc aagcagcaat gtatagttgc tctatgatca ataccatcca tcgaccaaac
     4381 attaagctga ttaacagaca ctatatgagg aagttggctg agttcatggt gcaaagcgca
     4441 atagtctatc gaatcaggaa caccatctaa aaaaatgtta gcagttgcta ctaactttgg
     4501 aagagcttta gataatataa aagaagcaat ggcaattgat aataaaggat caagaatgta
     4561 ccaaggtttc cattttaaga tcagtgacaa tatgataatg gctaaccacc caaggatatc
     4621 ttctaaaaag tgaaggctta atatttcttc attttttgtc tggttagagt gaatgatgaa
     4681 gctagcaaat ccattgatta taattgcaaa aatagctaaa acgaacatac cgtcataatt
     4741 aacaatagta ggatgccata attttggtat cgtttcaatc attactaata atgacccgct
     4801 aataagtatt agatttgttg ttagtgctcc taaaaggcta aatcgcttat agcctagtga
     4861 aaaatttgga ctctcttttt taacagcctt tctttctaag atagctgaga taccaatagc
     4921 aatggcatct ccaaaatcgt ggacagcatc tgctaaaata gcacccgaaa agaataatgt
     4981 tccaaaaata aattcaatga gggaaaaact aagatttaat ataaaaataa tggtgacttt
     5041 tttacttgct ggcataattt tct
//
LOCUS       DAUCWO010000082         4972 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUCWO010000082 DAUCWO010000000
VERSION     DAUCWO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.082"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            368..484
                     /locus_tag="VGY20_001571"
                     /pseudo
     CDS             368..484
                     /locus_tag="VGY20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(670..>4972)
                     /locus_tag="VGY20_001572"
     CDS             complement(670..>4972)
                     /locus_tag="VGY20_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ4274399.1"
                     /translation="KKESIPNKYEKGKKEELKRDAETKLREAQETTKQAIENDPWLSP
                     EQKKAQKENAKTRLDAGLKAVETTESLDKLKEVESDFLDKEKAESIPSQHQAGTPEVA
                     RKTFLDNFEKEAKKEIESIKNDATLTDAEKATAKAKVEAQLQEAKDKAKESKSFDDLK
                     NIQDKFNSELPHTTGKPLKDQQSDAIAELEKKQQEIEKAISEDKTLSKDEKEKQIADS
                     KAKLVAEKEKVSKAPDADAVKKALELGKQEIAKAYVPQNLEDHKKKLLAELKQKANDT
                     EKAIDFDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIQDSHV
                     KGDLEGVKNKAIEDLQKVHDETVAKINGDDTLDKATKEAQVKEADKALAAGKDAITKA
                     DDADKVGAAVTEHTPKIKAAHKTGDLKKVQEEANQALDKAAEKERGEINNDITLTAKD
                     KEQQLKEVETALTKAKAKVAEAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     VTDIKNQHKTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVKDVDAAK
                     ERGMAKLTEAKNADELDKAYGEGVTDIKNQHKSGDPIEARRGLHNKSIDEVAQATKDA
                     ITADTTLTEAEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGK
                     GLDVRKDEHKKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKD
                     ADEADKAYGDGVTAIENAHVIGKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQR
                     KEQLLGVDTEYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEF
                     LLKEADKVTKLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGI
                     ENINNQYQHGDGVDVRKATAKGDLEKEAAKVKALITNDPTLTQADKDKQTAAVDAAKN
                     TAIAAVDKATTADGVNQELGKGITAINKAYRPGEAVKAHKEAAKVKALIAKDPTLTQA
                     DKDKQTAAVDAAKNTAIAAVDKATTAEGINQELGKGITAINKAYRPGEGVKARKEAAK
                     ADLEKEAAKVKALITNDPTLTKADKAKQTEAVAKALKAAIAAVDKATTAEGINQELGK
                     GITAINKAYRPGEGVKARKEAAKANLEKVAKETKALISGDRYLSETEKAVQKQAVEQA
                     LAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNEAKQAAIEAL
                     KQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQSIASVKEAKAKGIA
                     AIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVVSLLLGMVLYSKKKESKD"
BASE COUNT         1156 a         1046 c          878 g         1892 t
ORIGIN      
        1 ttatcaaaaa tgacaaaaaa aataataatt gattaatata gaaacaatta ttatctaata
       61 tactgaattt tataatagat tctatcaata actatgtgta aacaatcaag tagaaatgtc
      121 tttattaagc gaatcaatca aggttgtgga gtaaatgctg gactagattt catgtagaaa
      181 ttagtaaaag ttaggagatt ttttggtcgt ttcaaacgat aacatcatct tcttataaca
      241 agaacttcac tcagagataa actgttctaa tactttagta gcctcttttg agcttgtagc
      301 acgaaaattc gctcgaactg atttaaaata ggcattttat ttgctcgctt gatccctact
      361 gcgcgaccaa gaaacctttt attcactaga acaattaacc cttaaaacca aggaggaagt
      421 ccactggtgg aactaccatc accttcgcag taatcttaat tatcaaacac ccataactaa
      481 atgagtcatc gtttgaacaa aaaacgcctt ataaattctg tacagaaaac tgttgccttt
      541 tcaattgaac tttgaaatgt tttcaataca ctccgttctt gggcccttac gatccaaaaa
      601 accaaggtga tttcaaaatc accttggttt gaaaatatag tagaaagcaa tttggataaa
      661 gccaggtttt tagtctttac tttctttttt cttgctataa agcaccatac ctaaaagaag
      721 agacacaact cctaagccaa caagaacaat tgagtttgca tcacctgatt ttggaagatg
      781 gttcgctgac gatgattttg cgactgcctt attaggcaca taggctgcac ggatagcagc
      841 aataccttta gcttttgctt ctttcacaga cgcaatagat tgtgctgaac gaacagtcgc
      901 aatagccgtc ttaagcgcta atgatacatt ttctgattgt tcagcttttt gagcttcagt
      961 caatttagca tcagttgtaa tcttagcaag tgtttctgcc gcagcttgtt taagagcttc
     1021 aatagcagct tgtttcgctt cgttaagagc agctgttgct tgatcagtat ctttagctaa
     1081 accagcaaca tatgctgaac ggatagctac agtaccaagg ttttctgcca acttaacagc
     1141 ttcaactgtc ttagcagcct caacttgacc aagtgctttc gcaagagctt gctcaacagc
     1201 ttgtttttgg actgcttttt cagtttcgct caagtaacgg tctcctgaaa taagagcttt
     1261 agtttcttta gctacttttt caagattagc tttagcagct tctttacgtg ctttaacacc
     1321 ttcacctgga cggtaagctt tgttgatagc tgtgatgcct ttaccaagtt cttggttaat
     1381 gccttcagct gttgtcgctt tatcaaccgc tgcgatagca gctttaagag ctttagcaac
     1441 agcttctgtt tgtttagctt tatcagcttt tgttaaggtt gggtcgttag taataagagc
     1501 tttcacttta gcagcttctt tttcaagatc agctttagcg gcttctttac gtgctttaac
     1561 accttcacct ggacggtaag ctttattgat agctgtgatg cctttaccaa gttcttggtt
     1621 aatgccttca gctgttgtcg ctttatcaac cgctgcaatt gctgtattct tagctgcgtc
     1681 aaccgctgct gtttgtttgt ctttatcagc ttgagttaag gtcggatcct tagcaataag
     1741 agctttcact ttagcagctt ctttatgtgc tttaacagct tcacctggac ggtaagcttt
     1801 gttgatagct gtgatgcctt taccaagttc ttggttaaca ccatctgctg ttgtcgcttt
     1861 atcaaccgct gcaattgctg tattcttagc tgcgtcaacc gctgctgttt gtttgtcttt
     1921 atcagcttga gtcaaggttg ggtcgttagt aataagagct ttcactttag cagcttcttt
     1981 ttcaagatcg cctttggcag tcgctttacg aacatcaacg ccatcgccat gttggtattg
     2041 gttgttgatg ttttcaatac ccttaccaag agcatcattg atagcatcag ctgtttgagc
     2101 atcatcaaca gacttgattg cgtctaactt agcttgttca actttgttga tttgatcaac
     2161 tttttggtca tgagtcaagg ttggatcatc attgattagt ttcgtcactt tgtctgcttc
     2221 tttaagaaga aattctttgg cagcattacg acgatcatta aggttttgac cttctttgta
     2281 ctgtgccagg atgtcacgaa caccgtcact atatgcttta tcaacacctg cagcatcctt
     2341 agctgcatca atattctcga tacctttagc atactctgta tcaacaccta ataactgttc
     2401 tttacgttga tcatcagtaa gcgttttgtc ttcaataatg agagcttttg tcttagcagc
     2461 agcttcagca aggtctttct tagcaaggtc tttacgagct tcgatacctt taccaataac
     2521 gtgggcattt tcgatcgctg tgacaccgtc accgtaagct ttgtctgctt catctgcatc
     2581 tttagcttca gcaatcttat cagtcgcaag ttcaagctct ttttgaacct ctgctttttg
     2641 ttgttctctc acttctggtg ttaaggttgg atcagcctca atttcagcag tgacacgttt
     2701 agcaacagct tcaagagctt tcttgtgctc atctttacga acgtcaagac ctttacctga
     2761 cttgtgttgg ttcttgatgc tggttacacc gtcaccgtac gctttgtcta aagcatcagc
     2821 atctttagcc ttagcaagtt cttctttagc tttagttgct tctttatcaa cattgccacg
     2881 ttgtgtttct ttttcagctt cagtcaaagt cgtatctgct gtgatagcgt ccttagttgc
     2941 ttgcgccact tcgtcgattg acttgttgtg taatccacga cgagcttcga tagggtcacc
     3001 tgatttgtgt tggttcttga tgtctgtaac accttcaccg taagctttgt ctaactcatc
     3061 agcattctta gcttcagtaa gcttagccat gcctctttct ttagcggcat ctacgtcttt
     3121 aacttgttta gctttttctt cagtcgtcaa agtagggtct ttttcaatcg catctttagt
     3181 ttcttgagcg actctatcaa gttgcttacc atgagcctca cgacgagcta cgacagggtc
     3241 acctgtctta tgttggttct tgatgtctgt gacaccttta ccaagagcat cgtcaacctt
     3301 gtcagctgtg tcagctgcgt taacagcttc tatcgctttc ttaagttcag cgttaacagc
     3361 tttagattgc tcttcttttt ctttgcttgt caaagttgga tcagctgaaa tcttgtcttt
     3421 cgttgcttta gctgcttctt caagtttagc gacttgacct gacttacgag cacctaagtc
     3481 ttgacctgct ttatgaacgg catcaattgt tgctacgcct ttatcacgtg cgtcattgat
     3541 agcgtctgct gtcttagctt ctgccacttt agctttagct ttagtaagag ctgtctcaac
     3601 ttctttcagt tgttgttctt tgtcttttgc cgttaatgtg atatcattat tgatttctcc
     3661 acgttctttt tcagctgctt tgtcaagagc ttggttagcc tcttcttgaa cttttttaag
     3721 atcaccagtt ttatgtgctg ctttaatttt tggtgtgtgc tctgtaacag ctgcacccac
     3781 tttatcagca tcatctgctt tagtgatcgc atctttacct gctgccaaag ctttgtcagc
     3841 ttctttcact tgagcttctt tagtagcttt gtcaagggta tcatcaccat ttattttagc
     3901 aactgtttcg tcatgaactt tttgaagatc ttcaatagct ttattcttaa caccttcaag
     3961 gtctccttta acgtgagaat cttgaatagc tttcttaagt tctgggactt ttttatctag
     4021 ttcttcacga ttgtcaattg ctccaacagc ttctgttgct ttttgaagtt cttcttttgt
     4081 tttcgctttt tgctcttctt tttctttcgc tgtcaaagtt ttatcgaaat caatcgcttt
     4141 ttcagtatca tttgcttttt gcttcaactc tgcaagaagt tttttcttgt gatcttctaa
     4201 gttttgtgga acataagcct tagcaatttc ttgcttacct aactcaagag ctttcttaac
     4261 agcatcagca tcaggtgctt tagaaacttt ttccttctca gcgactaatt tcgctttaga
     4321 atcagcaatt tgtttctctt tttcatcttt tgacaatgtc ttatcttctg agatagcttt
     4381 ttcaatttct tgctgcttct tctctaattc tgcaatagca tcagattgtt gatctttaag
     4441 cggcttacct gttgtgtgtg gtaattcaga attgaactta tcttgaatgt tcttcaaatc
     4501 atcaaacgat tttgattcct tagccttatc tttagcttct tgaagttgtg cttctacttt
     4561 agctttagct gttgcttttt cagcatctgt taatgtagcg tcatttttaa ttgattcaat
     4621 ctctttttta gcttcttttt caaagttatc taagaaagtt ttgcgagcaa cttcaggagt
     4681 tcctgcttga tgttgagatg gaatagattc tgctttttct ttatcaagga agtcactctc
     4741 aacttcttta agtttatcta agctttctgt agtttcgaca gcttttaaac ctgcatctaa
     4801 tcttgtttta gcgttctctt tttgagcttt tttctgttcc ggactcaacc atggatcatt
     4861 ttctatagct tgtttagtag tttcttgagc ttctcttaat tttgtttcag cgtctctttt
     4921 taattcttct ttcttccctt tttcatactt gttgggtatt gattcttttt ta
//
LOCUS       DAUCWO010000083         4960 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUCWO010000083 DAUCWO010000000
VERSION     DAUCWO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.083"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..684)
                     /locus_tag="VGY20_001573"
     CDS             complement(<1..684)
                     /locus_tag="VGY20_001573"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ4274400.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKYITYKELADQQILNDIEIIINTTPVGMINYEE
                     SLPINFDHLD"
     gene            complement(753..824)
                     /gene="shp3"
                     /locus_tag="VGY20_001574"
     CDS             complement(753..824)
                     /gene="shp3"
                     /locus_tag="VGY20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ4274401.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            904..1755
                     /gene="rgg3"
                     /locus_tag="VGY20_001575"
     CDS             904..1755
                     /gene="rgg3"
                     /locus_tag="VGY20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ4274402.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNPYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(2008..>4960)
                     /gene="smc"
                     /locus_tag="VGY20_001576"
     CDS             complement(2008..>4960)
                     /gene="smc"
                     /locus_tag="VGY20_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ4274403.1"
                     /translation="ELDTQLAPLEKQAKVAKQFLELDANRKQLQLDILVKDIDIAQER
                     QTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKKQVLSQESDQTQTTLLELTKLIA
                     DLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDGFQAEEKQRTEQLLHIDQQLCDV
                     KQQLNELSNALERFSSDPDQLMETLREEFVLLMQKEAALSNQLTALKAHLDKEKQARQ
                     HKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEMLLQNYQEGDKRVQELERDYQLN
                     QERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVRAVLQSQKKLGGIIGAVSEHLSF
                     DSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKKNRQGRATFLPLTTIKARSLSEH
                     YHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSSTAIFETIDQANIAARLLGYKVRI
                     VTLDGTELRPGGSFSGGANRQSNTTFIKPELEQISEELTRLVEQLKITEKEVAALQSD
                     LIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQEDSKALLAALDQSQTTHSDESL
                     LAEQARIEEALTAIAKKKSALTCDIDDIKENKDLVRQKTQNIHQALSQARLQERDLLN
                     EKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQDSIQRLPQWQKQLQDATEHKSG
                     AQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTFIRRQTKLETHLEQVANRLRAYA
                     KSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQKTIRALGPINSDAITQYEEVHER
                     LTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKMTFEAIRKSFKETFTQMFGGGSAD
                     LVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKALSALALLFAIIRVKTIPFVILD
                     EVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGTMAAADSIYGITMQESGVSKIVS
                     VKLKEAQEMTN"
BASE COUNT         1454 a          931 c          779 g         1796 t
ORIGIN      
        1 gtccaaatga tcaaaattta taggtaaact ttcttcataa tttatcatac caactggtgt
       61 agtatttatt atgatttcta tatcatttaa aatttgttga tcagccaatt ccttatatgt
      121 aatatacttt aaatcaggga aactctttat caaacttctt gtattctcac taattggctc
      181 cctgtataca actgttggtg agatttgtag tttttgtaaa gtgaaaatag cagctctagc
      241 agtaccgcca gtgcctaaaa cacaacattt tgttatatgg ctaatattaa agaaaactaa
      301 tgtattataa atacctttcc aatcagtatt agttcctgta attatatttc cagatttata
      361 aatattgtta acagatcctg ttatctctgc tactctgtct aaattgtcaa tatacctcat
      421 tacctctctt ttataaggta aagttatatt taatccaata cattttggat catttatcag
      481 atgtttgatt ttatcttcta aacacactga gtctaccaaa atttttttat gtttatattc
      541 atccagaact cctatgttgt gatttagata ttcaaataat tcatctgata tagaatgttc
      601 aactggatta ccaagcaatg cagaatacat aaatttcatc ctcctacgtt taattagttt
      661 actactaaca taaagtacaa gcattacaca gagctaacca ttacctataa gtctttagag
      721 tatctaaaga cttaggtata cacctaagta acttacccac caacaataat gataatatcc
      781 attgctaaaa ttaaaatcgg caaaaatttt gaaattttct tcataagtgg acttcctttc
      841 agtttttatt tattgtacaa taaaaaaaaa cggaattgat ttttgttggg aaagtgggaa
      901 aatatgaaat caaaacttgg tgccacactt agaaaagttc gaaaaggaaa gcaaattagt
      961 ctgtgcttcg tggcagatga acacctttca aaatcacaaa tctctcgttt tgagcgcggg
     1021 gagtcagaga tatcgtgtat ccgactgatc aatattctag acaaattaca cattactttg
     1081 gatgagttcc ttattttaca tgatgaagac tataccaatt ccgaaacatt tgctaacctg
     1141 gtacagtata ttcgtaagca atattcatca caaaatatca ataatatagc atgtttactt
     1201 tccgacactt ctgattacac tctgaattct tttgagaaaa caatggtaaa gtccatttta
     1261 cacacaatgg attcaagcat cattccatca gatgatgaat tacttcaact aacagattat
     1321 ctctttaaag ttgaaaaatg gggctactat gaaatccttt tattgggtaa ctgtgtacga
     1381 acaattaact acaaccccta cttcctattg acaaaggaga tgttaaataa ttacatttac
     1441 tcttcacgga acaaaactaa taaacgaatt gtcactcagc ttgctataaa ttgtttcatt
     1501 cttagcatca ataaggagga attttctaat tgctcctatt taattgatga gataaaaacc
     1561 ttgttagata atgaattaaa cttctatgaa cagacagttt ttctctatgc aacaggttac
     1621 tttgaattta agcgccaatt agaaactgga gttgaaaaga tgaaacaagc catacaagtt
     1681 cttgatattt taaatgaaga caagttaaaa ttacattacg ctgaccattt tgataaacta
     1741 gtcaacaaag attaagtaaa atcattcttt accttttaac gaaacgccaa tcaagggtgt
     1801 ctcaataaaa aatcatctac tctgacaact tttctctttt acctatcgac agctaaaaag
     1861 ccttggaaac tgctatttcc aaggctttct ttgtactcta ttgtatcaat tagcttattc
     1921 cgatttctgt tgctattagt ttaatcatga aatctcctaa cttatggccg aacatccaaa
     1981 aaaacagaag gaactgcctt ctgtttttta attggtcatc tcttgagctt cttttaattt
     2041 tacagataca attttagaga cccccgattc ctgcatggta attccataaa tactatctgc
     2101 tgcagccatt gttcctttac gatgggtcac gacaataaat tgactatctt tgtcaaagcg
     2161 attgaggaaa tccccaaagc gcttaacatt tgcttcatca agagccgctt ctacctcatc
     2221 taaaatcaca aacggaatcg ttttaacacg tataatagca aacaataaag ccagtgctga
     2281 caaagctttt tctccgcctg acatcaagtt aagagattgg attttcttac caggaggctg
     2341 cacagaaatc tctataccag cagacaagag gtctgtttcc gttaaaacca aatcagcaga
     2401 accccctcca aacatctgag taaaggtttc cttaaaactt tttcgaattg cctcaaaagt
     2461 cattttgaaa cgagccttca cttcgctatc catactatta atagtctcta gcaaaagatt
     2521 tttagctttt gtcagatctg tcttttgact tgttaaaaaa gtcaaacgct catgcacttc
     2581 ctcatactga gtaatagcat cactattaat tggtcctagt gcacgaatag tcttttgtaa
     2641 atggtgtaat ttttctttgg cactttctaa gtgatcaata ctatttgtta cctcctttgc
     2701 gtctgctagt gtcatttgaa aatcttctga taaactttta gcataggctc gtaagcgatt
     2761 agccacttgc tctaaatgtg tctctaactt ggtttgacga cgaataaatg tgtcattttt
     2821 ttcgctttct ttggttattt tttctgctgt ttcttctaag cgggcctcat agtcctctat
     2881 ttcaaatctt aattggacta aacgtttttg agcaccagac ttatgctctg tggcatcttg
     2941 taactgtttt tgccactggg gtagtctttg aatgctgtct tgacttacgt tgttgtttag
     3001 tatgctttca agtttgagaa tattttgctg gcattgcttc agctgagttc ttagacgaga
     3061 ttgattagct tgctcaaatt tcttttcatt aagtaaatct ctttcttgca aacgagcttg
     3121 agataaagct tggtgaatgt tttgtgtttt ttgcctaacc aaatctttgt tttctttgat
     3181 atcatcaata tcacaagtta aggcactttt tttcttagca atagctgtta atgcttcttc
     3241 aatacgagct tgctctgcca ataaagattc atcagaatga gtcgtctgac tttgatcaag
     3301 ggcagctaat aaagcctttg aatcttcttg tttttcttgc aattgctgat aagccatttg
     3361 agctctttgt tcagctaagc gtgcctggtc accggctaat ttcaattgtg tcagttcttc
     3421 tttttttgct atcaaatcgc tttgcaaggc agctacttct ttttcagtta tttttaattg
     3481 ctcaactaat cgcgttaatt cctcactgat ttgttctaac tctggtttga taaaagttgt
     3541 attgctttgt ctattagctc ctcctgaaaa agaaccacct ggtcgtaatt ctgttccatc
     3601 aagagtaaca attcttacct tatatcctaa taagcgcgct gcaatattag cttggtcaat
     3661 agtttcaaag atagcagtac tagataaaag gttttggata atagctgaaa gactatcatc
     3721 atagcgaacc aatgattctg cagttcccag ataaccttca caagtcgcta attgacgatg
     3781 atagtgttct gacaatgatc tagcctttat agtagttaaa ggtaaaaaag ttgctcgtcc
     3841 ttggcgattt tttttcaaat aagcaattgc tctttttgct gcagcttcgt ctgttacaat
     3901 aatatgctga ctattagcac ctaatgcaat ttcaagagct gtttggtaat cagaatcaaa
     3961 agaaaggtgt tcactgactg cacctatgat ccctccaagc tttttttgtg attgaaggac
     4021 agctcgtact cccgcataga attgactatg acttttttga atagattcta agctagcctt
     4081 acgagcttct tttccttttt tttgatccaa caagtcaaat agtctttctt gattcagttg
     4141 gtaatctctt tctaattcct gaacgcgttt atcaccttct tgataatttt ggagcaacat
     4201 ttcgacttgc tctttttgag ctttataatg agcttgagct ttttgagact cgtcattaag
     4261 ttgatcaagc ttagtaacaa gtagctgata ttcttgagcc ttatgttgac gagcctgttt
     4321 ttctttgtct aaatgggctt ttaaagctgt cagctgattt gataaggccg cctctttttg
     4381 catcaataat acaaactctt ctcttagagt ttccattaat tggtcgggat cacttgaaaa
     4441 acgctctaat gcattgctca attcgtttaa ctgttgtttg acatcacaaa gttgttgatc
     4501 aatatgcaat aattgttctg tacgctgctt ttcttctgct tgaaaaccat ccaattgttc
     4561 ttgtaactgt tcgagatgtt ttttggcttc tgcctttttc tctgcttctt ggccactttc
     4621 tagcttaacc aattcaattt gcttttccaa gtcagctatt agcttggtta attctaataa
     4681 tgttgtttgg gtttgatctg actcttgact aagaacctgc tttttttgct tgaatttttg
     4741 gtaatcttct tccatagatt gacgcttagc gtaataagat gctaagtctt gttgtaaagc
     4801 agctaaagct tcagtatctt tagtctgtct ttcttgagct atatcaatat ctttgactag
     4861 aatatctagt tgtagttgct tacgattggc atcaagttct aaaaattgtt tagctacttt
     4921 agcttgcttt tcaagtgggg ccaactgcgt gtctagctca
//
LOCUS       DAUCWO010000084         4928 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUCWO010000084 DAUCWO010000000
VERSION     DAUCWO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.084"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            43..261
                     /locus_tag="VGY20_001577"
     CDS             43..261
                     /locus_tag="VGY20_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ4274404.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            281..2440
                     /gene="nrdE"
                     /locus_tag="VGY20_001578"
     CDS             281..2440
                     /gene="nrdE"
                     /locus_tag="VGY20_001578"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ4274405.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITELASIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSNIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYADGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            2644..3603
                     /gene="nrdF"
                     /locus_tag="VGY20_001579"
     CDS             2644..3603
                     /gene="nrdF"
                     /locus_tag="VGY20_001579"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ4274406.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            3578..4891
                     /locus_tag="VGY20_001580"
     CDS             3578..4891
                     /locus_tag="VGY20_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ4274407.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVALT
                     SLWSQRCNLSKADQKLLLACASGAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADARSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVTKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
BASE COUNT         1462 a          998 c          881 g         1587 t
ORIGIN      
        1 ccatttaaga tggaaccatt actaatttta gaaggacaag acatgattac agtatattcc
       61 aaaaataatt gcatgcagtg caaaatgact aagaaatttt tagaacaaca cggagccaac
      121 ttccaggaaa ttaatatcga tgaacatcct gaaaaagtgg attatgtgaa aagtcttggc
      181 ttcacatctg ctcctgttat cgaagcagat aacctcgtat tttctggctt ccaacctgca
      241 aagttaaaag aattgattta ataagacaag gaaaaataat atgagtctca aagatcttgg
      301 cgatatttca tattttcgcc taaataatga aattaatcgt cctgttaatg gtaaaattcc
      361 acttcataaa gacaaagaag ctttaaaagc ttttttcgct gaaaatgtgc tgccaaacac
      421 catgtctttt acttccatta cggaaaaaat tgagtattta atctcaaatg attacattga
      481 atcagctttt attcagaaat accaccctga atttattact gaattagcta gcataatcaa
      541 atcagaaaat tttcgcttta aatcatttat ggcagcctac aagttctacc agcaatacgc
      601 cttaaaaaca aatgatggag agcattattt agaaaacctt gaagaccgtg tcttgtttaa
      661 tgctttgtat tttgcagatg gtcaagaaga cttagcaaaa gatttagccg ttgaaatgat
      721 taaccaacgt taccaaccgg ctactccttc ctttttaaat gctggtcgaa gccgtcgtgg
      781 tgaattggtc tcttgtttct tgattcaagt aactgatgac atgaactcta tcggacgttc
      841 tatcaactct gctttgcaat tatcccgtat tggtggaggt gttgggatta ccttgtctaa
      901 cctccgtgaa gctggcgcac caatcaaagg ctatgctggt gcagcctcag gagttgttcc
      961 tgttatgaaa ttatttgaag atagtttttc ttattcaaat caacttgggc aacgtcaagg
     1021 agctggtgtt gtttacctaa atgtttttca tcctgatatc attgctttct tatctactaa
     1081 aaaagaaaat gccgatgaaa aggtgcgtgt taaaaccttg tcactaggga ttaccgttcc
     1141 tgataaattc tacgaattag ctcgtaaaaa cgaggacatg tatctcttta gtccttacaa
     1201 tgttgaaaaa gaatatggca ttccctttaa ctatctcgac attaccaata tgtacgatga
     1261 gttagtggcg aaccctaaaa ttactaagac taaaattaaa gctcgtgatc ttgaaacaga
     1321 gatttcaaaa ttacaacaag aatctggtta cccttatatc atcaatattg atacagctaa
     1381 taaagctaat cctatcgatg gaaaaatcat catgagcaac ttgtgttctg aaattttaca
     1441 agttcaaaca cctagcctta tcaatgatgc gcaagagttt gtagaaatgg gaactgatat
     1501 ttcatgtaac ttaggttcca ctaatatcct gaacatgatg acctcaccag actttggccg
     1561 ttctattaag accatgacac gtgccttaac cttcgtgacc gactcatcaa acatcgaagc
     1621 tgtcccaacc attaaacatg gtaacagtca agcacatact tttggtcttg gagctatggg
     1681 actacattct taccttgctc aacatcatat tgaatatggc agtccagaat ccatcgagtt
     1741 tactgatatt tactttatgc tcctgaatta ttggaccttg gtcgaatcca ataacatcgc
     1801 tcgtgagcgc caaactacct ttgttggctt tgagaactct aagtacgctg atggtagtta
     1861 ctttgataaa tacgttacag gacactttgt tccaaaatct gatttggtca aaagtctctt
     1921 taaagaccat tttattccgc aagcttcaga ttgggaggct cttcgcgacg ccgttcaaaa
     1981 agatggtctt tatcatcaaa accgactagc agttgctcca aatggctcta tttcttatat
     2041 caatgactgc tctgcttcta ttcacccaat cacacaacgc atcgaagagc gtcaagaaaa
     2101 gaaaattggt aaaatctact atcctgcaaa tggtttgtct acggatacca ttccttacta
     2161 tacatctgct tacgatatgg acatgcgcaa agttattgat gtctatgccg ctgcgaccga
     2221 acatgtggac caaggcttgt cattaactct attccttcgt agtgagttgc ctatggagct
     2281 ttatgagtgg aaaacacaaa gcaaacaaac cactcgtgat ttatccatct tacgaaacta
     2341 cgctttcaat aaaggcatta aatctatcta ctatatccgt acctttacgg atgatgggga
     2401 agaagtgggc gcaaaccaat gtgaatcttg tgtcatttaa ttggttaaga agtaatgatg
     2461 ctaaagcatc tattacttct acggcagtcg ctttaggcga tctgcctaaa ccactcacta
     2521 actcattcgt tacccttttg aatctattta aacttattat taccggtaga actttggtcg
     2581 gttctactgg tttatctcga tatccttaac gcactttact actctttagg agatcatttt
     2641 tctatgacta catattatga agcaattaac tggaatgaaa tcgaagacgt cattgacaag
     2701 tcaacttggg aaaagttgac cgaacaattt tggcttgata cccgtatccc tttatcaaat
     2761 gacttagacg attggcgcaa actttctctt caagaaaaag atcttgttgg caaggttttt
     2821 ggaggattaa ccctacttga taccatgcaa tcagaaactg gtgtcgaagc tattcgtgct
     2881 gatgttcgca ctcctcacga agaagctgtc ttaaacaata ttcaattcat ggaatcagtt
     2941 cacgctaaat cttattcgtc tatcttttca accttaaaca caaaaaaaga aattgaagag
     3001 atctttgagt ggactaataa taatgagttt cttcaagaaa aagcacgtat tatcaatgac
     3061 atttacgcta atggagatgc cttacaaaag aaagttgcct caacatatct cgaaactttc
     3121 cttttttatt ctggcttttt cacccctctt tactatttgg gaaataataa gctagcaaat
     3181 gttgctgaaa tcattaaact aattattcgt gatgaatctg tacatggtac ttatatcggt
     3241 tacaaattcc agcttggttt taacgaacta ccagaagatg agcaagagaa ttttcgtgat
     3301 tggatgtatg acctccttta tcagctgtat gaaaatgaag aaaaatacac caaaacgctt
     3361 tatgatggcg tagggtggac tgaagaagtt atgacctttt tacgctacaa tgctaacaaa
     3421 gctcttatga atttaggaca ggatccttta ttcccagata cagcaaacga tgttaaccca
     3481 attgttatga atggtatttc aacaggaaca tcaaaccatg actttttctc acaggtaggg
     3541 aatggctatc ttcttggttc tgtagaagct atgtctgatg acgattataa ttatggactc
     3601 taatagtgct cacgagactg ataatttatc agtctctttt cttaactttt gttataatag
     3661 tcttatgaaa cgacactttt tactcttgac cttttacctc tttttaacag gtttaacagc
     3721 tggccttgtt gcttttatct taacaaaagc cattcacctg attcagtccc tcagttttgg
     3781 attcagtcaa ggttctttta gcactatgat tgctagtgtt ccaccacaaa gacgagctct
     3841 ttcactcctc tttgctggac ttcttgcagg tcttgggtgg cacctattgg ctaaaaaagg
     3901 aaaggacatt caatctattc agcaaatcat tcaagatgac atctcattta gtccctggac
     3961 acagttttgg catggttggc tccaattaac gaccgtatct atgggagctc ctgtcggccg
     4021 agaaggagct tcacgggaag tcgctgttgc cttgacgtct ctttggagcc aaagatgtaa
     4081 cttgtcaaaa gccgatcaaa aacttttatt agcttgcgct tctggcgctg cccttggtgc
     4141 tgtctacaat gctcccttag ccactatttt atttatcttg gaagctattc ttaaccgttg
     4201 gtcccttaaa aatatatacg ctgcctgcct aacgagctat gtggctgtgg aaacagttgc
     4261 tttattacaa ggacgacatg agattcaata cctaatgcct cagcaacatt ggacgctagg
     4321 aacccttatt tggtccgtcc tggctggtct tatcctttcc ctctttgctc acgcttacaa
     4381 acatcttttg gaacaccttc ctaaggctga tgccagaagt cagtggttta ttccgaaagt
     4441 tctcatcgct tttagcctta tcgcaggact cagcattttc ttcccagaga ttttaggaaa
     4501 tggcaaagct ggattactct tttttctcca tgaagaacca catctgagct atattagctg
     4561 gctacttgtt accaaagctg tcgctatctc tttagttttt gcttctggag caaaaggggg
     4621 taaaattgct ccttctatga tgcttggagg tgctagtggc ttactactag ctatccttag
     4681 tcagtacctt attcccttgt ctctatcaaa tacgttagcc attatggttg gtgcaacaat
     4741 ctttttaggt gtgatcaata agataccctt ggcggcacca gtttttctgg ttgaaattac
     4801 aggccaatcg ttactaatga ttatcccttt agcattagct aacctcattt tttattttag
     4861 ctatcagttt tatcgtttca tccttaaatg attagttaat tactttttta ataaaaagct
     4921 tcggatga
//
LOCUS       DAUCWO010000085         4711 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUCWO010000085 DAUCWO010000000
VERSION     DAUCWO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.085"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(364..779)
                     /locus_tag="VGY20_001581"
                     /pseudo
     CDS             complement(364..779)
                     /locus_tag="VGY20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1200..1733)
                     /locus_tag="VGY20_001582"
     CDS             complement(1200..1733)
                     /locus_tag="VGY20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ4274408.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1813..2589)
                     /gene="recX"
                     /locus_tag="VGY20_001583"
     CDS             complement(1813..2589)
                     /gene="recX"
                     /locus_tag="VGY20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ4274409.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2704..4059
                     /gene="rlmD"
                     /locus_tag="VGY20_001584"
     CDS             2704..4059
                     /gene="rlmD"
                     /locus_tag="VGY20_001584"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ4274410.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITAVKRNFAEAKLLTVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1428 a          741 c          987 g         1555 t
ORIGIN      
        1 cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt cgaatcccgt
       61 acggactata gctatttagt aactttggtt aaagtaatcg aactaacttg gttgattgta
      121 ttttaatgat aaaaagctta aatttgttat acaacaaatt taagcttttt attgtttctt
      181 tttaattgca gattgaggtt taaaagacac tttacaatgt tctcaagtca tgtgttgttg
      241 taggttgtat gcacatatta caaatcccat tatagaagaa attgtttaat taatggatag
      301 taatggatgg tatggagatc ttaatgtggt tttaatggta attttagcag ttatagtaag
      361 acaaaaggtg acttggaaaa gcttatgcag acgattctta actcccacaa tatcttctgt
      421 cagattttat tagacttctt cttggacatg agttggttta cgatttagta caaattgaga
      481 ttgagctagt gtttcagttt caattttgtc tgttttacga acgtgcaggc tattcaattg
      541 cttcttagct tctaaaggat taaaccgagt ataagcgtag ccattatctg cgaaaaaacc
      601 tgaagacgac gagaatagac attaatcgct ttaaggataa tctcagggtt atggacggtt
      661 ttcaagtttc caagaagatg attgaggccg atggtatcgt tttgtcttga atgattcctt
      721 attttagtgg tttcattttc aatacacgac gtcaagcgtc ctacatactt tgataaaatt
      781 ctacctaaaa gaggtgtctt gtcagttttg attacaacat caagtgccaa aagactttgc
      841 gacttaacaa gaaacttcta ctttaacgca aagaaaaagt agtgagtact ctctttcgtc
      901 ggagatttcc tcgctactaa acttagtatt ttttgatact ttccttaggt agtgcttaag
      961 gcagcgactt tctttggaat tccatgcctt atttttgaga tcctaggctc aaaaatgcca
     1021 agtggcgtca acattaacat tgacgccact tttaccacaa ggaaaaacat ctgttttggc
     1081 tcactccaca cgcagaaata ggaaattgga tgtcaaatca gcagagctgt tcatgctttt
     1141 tgagcctaaa actgagtttg tctgcgttct gagcactcct tgggagtgct ggacaactct
     1201 taacgattct tcagttcaag ataacgtttg taccaaatag tgatataggc cttggaaaat
     1261 ggtccttttt catgattaat ccaatcaacc aatattttga cattttcttt taggataaag
     1321 tccatatcgg ctgaatactg catctctttt ttatgaatct cgtattcatc aacatcaagt
     1381 aatctctttt caccatccgc aaagactttg acatctaaat cgtaatcaat gtatttaagg
     1441 gcctcagtgt ccatcatgta tggactagct aagttacagt aataagagac gccattatct
     1501 cgaatcatgg ctataatgtt aaaccagtat tttttatgaa agtaaacgat tgcaggttcg
     1561 cgggtcaccc atcgtctacc gtcactttct gtgacaagag tgtgatcatt aactccaatg
     1621 agggcatttt cagttgtttt tagtaccata gtatcgcgcc aagtacggtg caaactacca
     1681 tcatgcttat aactttgaat tgtaataaag tcgccttctt taggtaattt catgccttac
     1741 caactttcta caattaaact tatcctctat agtctatcat aaatgtgtga aaaaatcact
     1801 tcttttgtca gtctataaat aatttcttaa cttgcaatta atatcgtcgc tgttgtagcc
     1861 ttttcggtag agagcctgat agagcttttg ctttaaggta taaccatcat atttgcgact
     1921 gagtttacga tattgtttgt ctaattcttt gtcaagaaga tcttctattt cttgattatc
     1981 ttggtcaaaa ttaaggtgat tgaggctatg tttagctaga tcgtatgaaa agcctttggt
     2041 cagtaatgct tgggtgattt tatctttcaa ggcttttggt ggcaattttt cttgatattt
     2101 gtcaaagagt ttttggctta ctttttgagc gagttggcta aagtcagttt gagataagat
     2161 aggatcaatg tcatgacttg caatgccttt ttgtaataat ttttgtttta agacttgggg
     2221 acctttatca ccatttaact gattttggcg aatgtaggta tcagccaatt tggtgtcgtc
     2281 tatccattgt tcttcttgga gttgagtgat gatgtcagca ataatgtgtt cttcaatctc
     2341 atgcttgcgc aggtaatcag caacttgctt gttgctgcgt tgttgaaagg aaagaaaata
     2401 aagggctaaa tttttgccgt aggacagttg ggcaaaatgt ttcatgtctt caagctgatc
     2461 attgtcaagg actttatctt tactcaacat aaaccgaaca atagtatctt ctgttacata
     2521 aagggattcg tcattatcca attcgataag gtagaggcgt tttttctttt caattttagt
     2581 gattttcata ctttgattat atcaaaaatg gtaaaataga agatgaaaat actgctaaaa
     2641 atgtgttgct tgtgaatgct tttggtggtg agattaggat attagaggaa ctaaggggaa
     2701 gagatggtcg taaaggttaa acagaaaatt cctttaaaaa ttaagcgaat gggaattaac
     2761 ggtgagggga ttggttttta tcagaaaact ttggtatttg tccctggtgc tttaaaggga
     2821 gaagatattt tttgccaaat cacggcggtt aaacggaatt ttgcagaggc caagctgtta
     2881 acggtaaaca aggcttctaa gaatcgtgtc aaaccagctt gttctgttta tgaaacttgt
     2941 ggtggttgcc aaatcatgca tctagcctat cctaaacagc tagatttcaa agatgatgtg
     3001 attaggcagg cccttaaaaa gttcaaacca actggctatg aacaatttga gattagaccg
     3061 actttaggaa tgaaaaagcc agatcactat cgggccaaat tgcagtttca gttgcgatca
     3121 tttggcggga cggtcaaggc tggtttattt tcccagggaa gtcatcgctt ggttcctatt
     3181 gacaattgct tggtacagga tcaactaacg caagatatta tcaataagat tacgcagtta
     3241 gtggacaagt acaagttgcc catttacaat gagcgcaaga ttgctgggat tcggacgata
     3301 atggttcgta aggcacaggc tagtgatcag gtacagatta ttgttgtgag tagcaaagaa
     3361 gttcggttag cgaatttcat tggcgaattg actaaggcat ttcctcaggt taaaacggtt
     3421 gccttgaata gtaatcgttc taaatcgagt gagatttatg gggacgaaac ggaaattcta
     3481 tggggacaag aggcgattca tgaagaagtt ttagattatg gatttgcgtt atcaccgaga
     3541 gctttttacc aacttaatcc acagcagaca gaagttttgt atggtgaggt ggtgaaggcc
     3601 ttggatgtag ggagcaaaga tcatattatt gatgcctatt gtggggttgg ctcgattggc
     3661 tttgcttttg cagggaaggt caagtctgtg cgggggatgg atattatccc agaagcgatt
     3721 gaggatgcac aaaaaaatgc caaagccatg ggttttgata acgcttacta tgaggcaggg
     3781 aaggcagagg atattattcc taaatggtat aaacaaggtt accgcgcgga tgccatcatt
     3841 gtggacccac caagaactgg tttagatgat aaattattaa aaacgattct tcattaccag
     3901 cctaaacaga tggtttatgt gtcttgtaat acctccactt tggcccgcga tttggtgcag
     3961 ctaacaaagg tttatgatgt tcactatatc caaagtgtag acatgttccc ccatacagct
     4021 aggaccgagg ctgtcgtgaa gttacaaaaa cgtgtatgat tgagtggcaa taacaactca
     4081 agttttataa gctcaggtaa tgggtttgag caatagcttg acatagtagg gatgtcaaaa
     4141 acggtttact aaggatagtc atattattga taactctatg tttattttag aaacattcat
     4201 ggttaaaaaa gtttgttagt ttgtcactaa gtagggttta tttgaagagc aagtacacac
     4261 agacaatatt agggtaattg gttgttgaaa aaaagctgtt ttttgctata aattagaaaa
     4321 ctgagtggtc aggaggttac attaccagaa gttattttag tatgctaata tgaacgatga
     4381 tatagaggat ttatcctgat ttaatctttt aagctgtaag aaacctttgc aaaaactgta
     4441 ggctgtgtga gtctttatgt gttagatatt aaagataaat gcaacacttt tttataatga
     4501 gatcaacaac tgataaatct aagttgataa ccagtaaaat gatcggaatg tagaaaagtt
     4561 acttaatcaa gcttttcgta atttaactgt tagaatgaca gaacttatga taaaataaag
     4621 aatatgatta aaatgaaaaa gctcagttta tctattacgg agttcgaaat gaaaagaaaa
     4681 tcgagatgtg ataacatatt ttgaacgaga a
//
LOCUS       DAUCWO010000086         4584 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUCWO010000086 DAUCWO010000000
VERSION     DAUCWO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.086"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..642)
                     /locus_tag="VGY20_001585"
     CDS             complement(<1..642)
                     /locus_tag="VGY20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEQ4274411.1"
                     /translation="MKNISPLRYPGGKSQVYDYVRELVIANDAITYIEPYMGGMGIAL
                     KLLLNNNVHKIMVNDYDKAIYAFWYSVLNYTEQLIEKINTTPITIDEWKLQREVQKNK
                     NNCDDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIKLIA
                     SYKKQIKLYNMDAEKFIRLNITKTKNSFTFFDPPYYTKGPGLYTNFYNHENHHS"
     gene            complement(681..2345)
                     /locus_tag="VGY20_001586"
     CDS             complement(681..2345)
                     /locus_tag="VGY20_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4274412.1"
                     /translation="MEKIEFIQYRKLKNITIDFDKNINIIAGTNGTCKSSILHLISNS
                     HQRVSNSSRNPILSTIQKMNKIFNPKIESLARGDKKYNDPAPGMTGTLYTAFYENYSL
                     DFRRHNSRTVNNTERFAIKPKYKRGTSDRLPEIPIIYLGMFRLFSYGEWEGESKFHNI
                     INKLPKQYLDQLSMLYKKFTGYEIKFDGKLNNLGGIRSKTEFSTNTEGIDSNTISAGE
                     DNLLTILISLVSLKAYYESITPDTEKTVESILLIDELDASLHPEFQIKLLEEFKTFSK
                     DYKIQIFFTTHSLSLIDYSLDKKIGNIIYLIDQIDHVDLLKHLNKYSINALLQNKLVN
                     NVYVKQPIPVLLEDNEAKDFFQNLLQAYFERYQLSLESFFHIIDAKISSSAIKALAQD
                     RYTNQSSLRIISITDGDEQLGQQYLTHNLFSLPGNYSPEELIFKYLKEVLLDDYNFWQ
                     KQDIIAAGYTKKLVQESFITQLDELEAKLNQPGSHHGVKREENKKIYNCDTNFWNYVM
                     KYWIDDTNNQDEVLKFFDNLKIAFHKTAPFYNINPTSSWPFITGQL"
     gene            complement(2347..2730)
                     /locus_tag="VGY20_001587"
     CDS             complement(2347..2730)
                     /locus_tag="VGY20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ4274413.1"
                     /translation="MVKINEMLSKYNIKLFEFPDTMWKRSGFYYPDLRIIYVNQNLSQ
                     IEKEKVILHELGHIEHDPKQYQRLLLKYENQADRFMIRELIKNYLSSHNVVDFNWLQF
                     ATTYQISTTWGQQMIQEEFQNIVGV"
     gene            complement(2714..3055)
                     /locus_tag="VGY20_001588"
     CDS             complement(2714..3055)
                     /locus_tag="VGY20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274414.1"
                     /translation="MDEKDLKRIIESRYNSVRAFAIENDIPYTTMRSILERGVMNAKA
                     ETIFKICDILGINPESFAEEKTDWQATIDLSNLREKVVMFDGKPLSDKDVEKIEAIIK
                     ISLGVGNGEDK"
     gene            3253..3465
                     /locus_tag="VGY20_001589"
     CDS             3253..3465
                     /locus_tag="VGY20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEQ4274415.1"
                     /translation="MKNIEHIRKEKGVSLVDIADCLRLKSQTVREKINGDSDFKFGEA
                     LKVQQTFFPEFDIVYLFKERKESSVG"
     gene            3493..4212
                     /locus_tag="VGY20_001590"
     CDS             3493..4212
                     /locus_tag="VGY20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ4274416.1"
                     /translation="MNHLINVTLNENQEPVVSARDLHKELKVKTRFSEWVKQNFKILE
                     EGYDFTSVVGTTVVNNGAVREIQDYVLSLDAAKNLAMVSKTDKGKEVRKYFIQVEKDF
                     NSPEKIMARALLMADKKVHKLEAQIEADRPKVLFADAVEASETSILIGDFAKILRQNG
                     YNIGQNRLFAWLRENGFLIRKNGESYNMPTQRSMDMKLFEVKERTHQEPNGSIRISKT
                     TKMTGKGQQYFINKFLNEKVG"
     gene            4216..4383
                     /locus_tag="VGY20_001591"
                     /pseudo
     CDS             4216..4383
                     /locus_tag="VGY20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1476 a          728 c          778 g         1602 t
ORIGIN      
        1 actatgatga ttttcatgat tgtaaaagtt tgtatataat cctggtcctt ttgtgtaata
       61 tggagggtca aaaaaagtga aagaattttt tgttttggtg atatttaaac gaatgaactt
      121 ttcagcatcc atattataaa gtttaatttg ttttttataa gaggcaatta gctttatttt
      181 tttaatagtt ttttctttgt tgaaacggca atctaattta tagttcccat cttgttttaa
      241 cccaccaatt acaccagctt ttatgatacc tgagcgattt gttcgattga ggaataaggt
      301 tgagaaacct aaggttagca agtcgtcaca attattttta tttttttgga cttctctttg
      361 aagtttccac tcatcaatgg tgataggagt agtatttatt ttttcgatta gctgttccgt
      421 ataatttaat actgaatacc aaaaagcata gatagctttg tcatagtcgt taaccatgat
      481 tttatgaaca ttgttattta agagtaactt taaggctatt cccatccctc ccatataggg
      541 ttcaatatag gtgattgcat cattagctat aacgagttct cttacataat cataaacttg
      601 agacttacca ccgggatatc ttaacggact aatgtttttc ataagctatt cctcctttcc
      661 atatagaata cactattttt ttaaagttgg ccagtaataa atggccaact tgaagtagga
      721 ttgatattat aaaacggtgc ggtcttatga aaagctattt ttaagttgtc aaaaaatttc
      781 aatacctcat cttgattatt agtatcatct atccaatatt tcataacata attccaaaaa
      841 tttgtgtcac aattatagat ctttttattt tcttctcttt ttacaccgtg gtgagaacca
      901 ggttgattta attttgcttc tagttcatca agttgagtaa taaaagattc ttgaacaagt
      961 tttttcgtat atcctgctgc gataatatct tgcttctgcc aaaaattgta gtcatctaat
     1021 aagacttctt taagatactt aaaaataagt tcttctggag aataattccc tggtagacta
     1081 aataaattgt gtgttaaata ttgctgacct aattgttcat caccgtcagt gattgatatg
     1141 attcttagtg atgactgatt ggtatatcta tcttgggcta gtgcctttat cgccgaagag
     1201 gatattttag catctattat gtggaagaat gattctagtg ataattggta tctttcaaaa
     1261 tatgcttgaa gtaaattttg aaaaaaatct ttagcttcgt tatcttctaa taaaacaggt
     1321 ataggttgct tgacatatac attgttcact aatttatttt gtaacaaggc attaattgag
     1381 tatttattga gatgttttaa taaatcaaca tgatctattt ggtcaattaa atagattata
     1441 ttgcctattt ttttatctag tgagtaatca atcaatgaga gactatgagt agtgaaaaaa
     1501 atctgtattt tataatcttt tgaaaaagtc ttaaattctt ctaaaagttt aatttgaaat
     1561 tcaggatgca aagaggcatc taattcatca atgagtagaa tactttctac tgttttctcg
     1621 gtatcgggag taatactttc ataataagct tttagtgata caagtgaaat caatatggtt
     1681 aaaaggttgt cttctccagc agatattgta tttgagtcaa taccttcggt gtttgttgaa
     1741 aactctgttt tagatctgat gcctcccaaa ttgtttaatt taccatcaaa tttaatttcg
     1801 tatccggtga attttttgta aagcatactt agctgatcaa gatactgttt tggtaattta
     1861 ttgataatat tgtgaaactt actttcaccc tcccattctc cgtacgagaa tagccggaac
     1921 atccctaggt atattattgg aatttccggg agtctatcag aagttcctct tttatatttt
     1981 ggtttaatag caaaacgctc agtattatta acagttctac tattgtgtct cctaaaatct
     2041 agtgaataat tttcgtaaaa tgctgtgtaa agtgttccag tcataccagg agcaggatca
     2101 ttgtattttt tatctcctct ggccaacgat tcgattttag gattaaatat tttgttcatt
     2161 ttctgaattg ttgataggat tggatttctt gaagaattcg agactctttg atgggaatta
     2221 ctaattaaat gcagaatgga tgatttacaa gttccattag taccggctat tatattaata
     2281 ttcttatcaa aatcaatcgt aatgttttta agttttctat actgtataaa ttctattttt
     2341 tccatcttac actcccacga tattctgaaa ctcttcctgt atcatttgtt gcccccaggt
     2401 agtcgagatt tgatatgtcg tagcaaactg taaccaatta aaatcaacga cattgtggga
     2461 tgacaagtaa tttttgatca attccctaat cataaatcta tcggcttgat tttcatattt
     2521 tagtagcaat ctttgatatt gcttaggatc gtgttcgata tggcccagct catgtaaaat
     2581 aactttttct ttttctattt gtgatagatt ttgattgaca taaattatcc taaggtcagg
     2641 atagtaaaac cctgaccttt tccacattgt gtcgggaaac tcgaataact ttatgttgta
     2701 cttactaagc atctcattta tcttcaccat tccctactcc taaagatatt ttaataatag
     2761 cttcaatttt ctctacgtct ttgtcagaca aaggtttgcc gtcgaacatc acaacctttt
     2821 cgcgtagatt ggataggtca atagtggcct gccaatcagt tttttcttca gcaaaacttt
     2881 ctgggttaat tccaagaata tcacatattt taaaaatagt ttctgctttt gcattcatca
     2941 caccgcgctc taaaatggag cgcattgttg tgtatgggat atcattttca atagcaaaag
     3001 ccctaacgct attatatcta ctttcgataa tcctctttaa atctttttca tccatttttt
     3061 taatttcctt ttttcttttt ctactatata ataacatacg aaaaatcata tttcaagaca
     3121 aaaaaataac aaaaaattta aaaaaactgt tgactgtata cgaaaaatca tatataatta
     3181 aatcaagctt aaggaaacga cagcttttta aaataaaata atatacgaaa aatcgtattg
     3241 gaaaggaatg atatgaaaaa catcgaacac attcgaaaag aaaaaggtgt ttcattggtt
     3301 gatattgccg actgtttacg gttgaaatct caaacagttc gagaaaaaat taacggtgac
     3361 tctgatttta aatttggtga agcattgaaa gttcaacaaa ctttttttcc agaatttgat
     3421 atcgtctatc tttttaaaga gcgtaaagaa tcatctgtag gatgatatac gaaaattcgt
     3481 agaaaggaaa ttatgaatca tctaattaac gtaacactaa acgaaaatca agaaccagta
     3541 gtaagtgcaa gagacttaca taaagaatta aaagtcaaaa ctcgttttag tgagtgggta
     3601 aaacaaaact ttaaaattct tgaagaagga tatgatttta caagtgtagt tggaactacg
     3661 gttgtaaata atggggctgt cagagaaatc caagactacg ttctctcact tgatgctgcc
     3721 aaaaatttgg caatggtatc caagactgac aaaggcaaag aagtccgtaa atacttcatc
     3781 caggttgaaa aagacttcaa cagtcctgag aaaatcatgg caagggcgtt gctgatggct
     3841 gataagaaag tgcacaagct ggaagcacaa atcgaggcag accgtccaaa ggtgctattt
     3901 gctgatgcgg ttgaggctag tgagacatct atcttgattg gtgattttgc taaaatctta
     3961 cgtcagaatg gttataacat cggtcaaaat cgcttattcg cttggttgcg tgagaatggc
     4021 ttcctgattc gtaaaaatgg cgagagctac aacatgccga cacaacgaag catggacatg
     4081 aagctgttcg aagtcaagga acggacgcac caggagccaa atggcagtat ccgtatcagc
     4141 aaaactacca agatgacagg taaaggccag cagtatttta ttaataaatt tttaaatgag
     4201 aaagtggggt agagcatgag accaaaacga tacccatata gcgggaagaa aaacagaaaa
     4261 gcaaaagaca tcagcttgag actgatgtct aaagaggaat tatctgattt tagtcgtcag
     4321 attgctgaag ccactcgtga taatgtcgaa ccatttcgag agccgcaaag tatgatgagc
     4381 taagtattaa ctttgcaagg tttacagatt ccccttcgct ttctttcgca tatctattgt
     4441 aatggtactc aataacttcc agaatttctt cggttttacc attgcttaga gattgggcga
     4501 aattgttgaa atctttattc atataatcac cttttttcga gatgattata tcaaaaaagt
     4561 ccgacgggaa tcggactcaa aaca
//
LOCUS       DAUCWO010000087         4572 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUCWO010000087 DAUCWO010000000
VERSION     DAUCWO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.087"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            17..727
                     /locus_tag="VGY20_001592"
     CDS             17..727
                     /locus_tag="VGY20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4274417.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            720..2072
                     /gene="vicK"
                     /locus_tag="VGY20_001593"
     CDS             720..2072
                     /gene="vicK"
                     /locus_tag="VGY20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ4274418.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            2076..2885
                     /locus_tag="VGY20_001594"
     CDS             2076..2885
                     /locus_tag="VGY20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4274419.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            3339..4031
                     /locus_tag="VGY20_001595"
     CDS             3339..4031
                     /locus_tag="VGY20_001595"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ4274420.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            4032..>4572
                     /locus_tag="VGY20_001596"
     CDS             4032..>4572
                     /locus_tag="VGY20_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4274421.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIK"
BASE COUNT         1432 a          713 c         1001 g         1426 t
ORIGIN      
        1 tttctgaggt atagacatga aaaaaatact tattgtggat gatgaaaaac cgatttctga
       61 cattattaag tttaatttga caaaagaagg ttatgacatt gttacagctt ttgatggacg
      121 cgaagcggta accatttttg aagaagaaaa gccagattta attattcttg atttgatgct
      181 ccctgagttg gacggtcttg aagtagccaa ggaaattcgt aaaaccagtc atgtcccgat
      241 tattatgttg tcggctaaag atagtgagtt tgacaaggtt attggacttg aaattggggc
      301 tgatgattac gtgaccaagc ccttttctaa tcgggaattg ctggcgcgtg tcaaggctca
      361 tctgcgtcgt accgaaacta ttgaaacggc tgttgcagaa gaaaatgctt cttcaggtac
      421 acaggaacta accattggta atttacagat tttaccagat gcgtttgttg ctaaaaaaca
      481 tggtcaagag gtagagttga ctcatcgtga atttgaacta ttgcatcatc tagctaacca
      541 tatgggtcag gtgatgacac gagaacactt attggaaatt gtttggggat atgattattt
      601 tggcgatgtg cgcacggttg atgtgactgt tcgtcgtctc cgtgaaaaaa ttgaagacac
      661 accaagtcgt cctgagtata ttttaacaag acgtggtgtt gggtactaca tgaaatctta
      721 tgactagaga tatcattgga aacttatcca catttgaatt agcgatcctt atcttacttg
      781 tttttgttgc tttttacttt atccatcttg cggtgcgtga ttaccgaaat gcacgtatta
      841 ttcggatgat gagccataaa atccgagact tgattaatgg tcgctatact gatataatcg
      901 acgaaaaagc agacattgag ttaatggagc tttcagacca gttaaatgac ctgtcagatg
      961 tttttcgctt gacgcatgaa aatcttgccc aagaaaaaaa tcgcttggca agtattttgg
     1021 cttatatgtc agatggtgta cttgctacag accggtctgg taaaatcatc atgattaacg
     1081 agacagctcg caagcaatta aatttaagta aagaagaggc actaaagaaa aacattacag
     1141 atttgttaga aggtgatact tcatatacct accgtgattt ggtatccaaa acaccagtgg
     1201 taactgttaa tagccgaaat gatatgggtg agtttgtttc attacgcttg cgttttgcgt
     1261 tgaataggag agagagtggt tttatttcgg gcttggttgt ggtgctccat gacaccacag
     1321 aacaagaaaa agaagaacgt gaacgccgtc tttttgtttc taatgtaagt catgaattaa
     1381 ggaccccttt aacttcggtt aaatcctact tggaggctct tgatgaaggt gcacttaaag
     1441 aagatattgc tccaagtttc ataaaagttt ctcttgatga aactaatcgg atgatgcgta
     1501 tgatttcaga tcttttaaac ctgtctcgga ttgataatca agtaacccaa ttagcagtag
     1561 agatgactaa ttttactgct tttataactt ctattttaaa cagatttgat ttggttaaaa
     1621 atcagcatac aggtacagga aaagtctatg aaattgtaag agattaccct attacctctg
     1681 tctggcttga aattgataat gataaaatga cacaggttat cgagaatatt ttgaacaatg
     1741 ccattaagta ttctccagat ggtggaaaaa ttacagtccg tatgaaaaca acagataccc
     1801 aattaattat ttccatttca gaccaaggac taggtatccc taaaacagat ttgcctctta
     1861 tttttgatcg gttctatcgt gtagacaagg caagaagtcg tgcccaagga gggaccggtc
     1921 taggccttgc cattgctaaa gaaatcatca agcagcacca tggctttatc tgggctaaga
     1981 gtgactatgg taaaggatcg acctttacta ttgtcttgcc ttatgaaaaa gatgcagcca
     2041 tctatgaaga atgggaggaa gatgtagact aatcaatgaa tgagagtggt tttaaatata
     2101 gtattttagc atctgggtcc acaggaaatt gtttttattt agaaacacct aaaaaacgct
     2161 tattgattga tgcagggctt actggtaaga aaattaccag tcttcttgct gaaattgacc
     2221 gcaaacctga ggatttggat gctattttga ttacccatga gcactctgac catattaagg
     2281 gtgtaggcgt tatggcccgc aaataccatt tggatatcta tgctaatgaa aaaacatggc
     2341 agctgatgga tgagtgcaat atgctaggta agcttgatgc ctcacaaaaa catattttcc
     2401 aaagggataa agttttgacc tttggagatg ttgatatcga aagttttgga gtcagccatg
     2461 acgctattga tcctcaattt tatcggatta tgaaagataa taagtctttt gtgatgttga
     2521 cagatacagg atatgtcagt gataggatga caggtattat tgaaaatgca gatggctact
     2581 tgattgaatc caatcatgat attgaaattt tgagatcagg ctcttatcct tggagcttga
     2641 agcagcgtat tttatctgat ttaggacatt tatcaaatga agatggagcg ggagccatga
     2701 ttagaagcct ggggtacaat accaaaaaaa tatacctagg tcatctgagt aaagaaaata
     2761 acatcaaaga gttagcgcat atgacgatgg tcaatcaact ggctatggca gatttagcag
     2821 taggtacaga ctttacggtc catgatacct ctccagatac tgcttgtcca ttaactgata
     2881 tttgagataa gaaactaaaa gagaatatgc aaagtagcgc tgattggtga tataccagtc
     2941 agggctattt tttgatgtct taaagcgtat tgtcttctga gttcaatgaa ctctcattgg
     3001 ttatccttta gagcatcaat agacttaaat tagcctgtag ttttggcttg tctgatctat
     3061 ttatcaaagg tgttgaggat tataaagata aggtagtttt ttagttgttc agactaaaaa
     3121 caaaatagct ctatactatc tgtagtggga ctgtagtggg actgtagtgg gaaattcccc
     3181 tcagatagtg tagagtcttt tgattgtatg agaaagtttc ctttgacggg tatttaagat
     3241 gtttaataaa tcacgaaaaa tccacttaga gtcggttttc taggtggatt tttggtatac
     3301 taaataaggt ctactgacaa atatgaaagg gattttttat gaaacagctt gaagagttac
     3361 tctcaacatc ttttgatatt caatttaatg atttgacttt actagaaaca gcttttaccc
     3421 atacctctta cgctaacgag catcgtctcc taaacgtttc acataatgaa cgcttggaat
     3481 ttttaggaga cgctgttcta caattgatta tttcagagta tttatttgca aagtatccta
     3541 aaaagacaga aggagatatg tctaagttac gttccatgat tgtacgtgag gaaagcttgg
     3601 caggtttttc acgattttgt tcttttgacg cttatatcaa actaggaaaa ggtgaggaaa
     3661 agtcaggtgg tcgccgtcgt gatactattt taggagactt atttgaagcc tttttaggtg
     3721 ctcttttatt agataagggg attgatgcag tacgtcgctt cttgaaacaa gtcatgattc
     3781 ctcaggtgga aaaagggaat tttgaaaggg ttaaagacta taaaacttgt cttcaagagt
     3841 ttttgcaaac aaaaggtgat gtggctattg attatcaagt cattagtgag aaaggacctg
     3901 cccacgctaa acaatttgag gtatcgatag ttgttaatgg tgctgtcctt agtaaaggtt
     3961 taggtaagtc taaaaaatta gcggagcaag atgctgctaa aaatgccctt gcccaactta
     4021 gtgaggttta gatgttttta aaagaaattg agttggaagg gttcaagtcc tttgcggaca
     4081 agactaaaat tgaatttgat aagggagtaa ctgcagtcgt aggaccaaat ggttcaggta
     4141 aaagtaatat tacagaaagt ttacgttggg ctttaggaga atcaagtgct aaaaatctgc
     4201 gtgggggcaa gatgccagat gtcatttttg ctgggacaca aaaccgtaat cctctaaact
     4261 atgctaaggt agcggttgtt cttgataatt ccgatcattt tataaaaact gccaagaaag
     4321 aaatccgtgt cgaaaggcat atttaccgaa atggagatag tgactatctg attgatggtc
     4381 gcaaggtaag gctccgtgat attcatgatt tgtttatgga tacagggctt ggtcgtgatt
     4441 ctttctccat tatttctcaa ggtcgtgtag aggaaatttt caatagtaaa cctgaagaaa
     4501 ggcgcgccat ttttgaagaa gcagctggtg ttttaaaata taaaacacgt aaaaaagaaa
     4561 ctcagattaa at
//
LOCUS       DAUCWO010000088         4337 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUCWO010000088 DAUCWO010000000
VERSION     DAUCWO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.088"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1246
                     /locus_tag="VGY20_001597"
     CDS             <1..1246
                     /locus_tag="VGY20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274422.1"
                     /translation="IGKNEFEILYKRSSDERANWYISHLLTHDFAVKELRLGTLESYF
                     LKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFLGILLVGNV
                     TTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQSV
                     EFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSRKSTIFKLACGLYDNYEGNIY
                     INGINLRSIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTG
                     VDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPS
                     SALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCISETGSFADLN
                     IKGMKFKNLLDKEIGATYDTSN"
     gene            1230..2474
                     /locus_tag="VGY20_001598"
     CDS             1230..2474
                     /locus_tag="VGY20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEQ4274423.1"
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFD
                     KSLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGI
                     ALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSG
                     ITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPML
                     ILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMA
                     WCYGMPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIY
                     DLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVS
                     GIVLFLLNPYSDEELLWQRIFI"
     gene            2493..>4337
                     /locus_tag="VGY20_001599"
     CDS             2493..>4337
                     /locus_tag="VGY20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="HEQ4274424.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     YDSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKV
                     SFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNI
                     SFSKPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRP
                     PICNINSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYK
                     YDNGGNYLKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEY
                     QLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEAL
                     LNGGHIEFYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFG
                     RFYGLSETVRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSVFTTSWDS
                     ENELRLEDIYIRYSDGKFHFTTIDGENELKFTMNNMLNSDSQSRVLRLLVDLSEFEYG
                     LSHWSLFPWDILAQERVYIPEILFEDIPIATAQWNLSVIREEIIHQKIF"
BASE COUNT         1624 a          487 c          806 g         1420 t
ORIGIN      
        1 aattggtaaa aatgaatttg agatactata taaaaggagt agcgatgaga gagcaaattg
       61 gtatattagt catcttttaa cacatgattt tgctgtcaag gagttgaggt taggtacgtt
      121 agaaagttat tttctaaaaa aatatgataa aaataatcaa ttatttatta atcaggaaaa
      181 tagtataaat agatctaaaa tgaaatacgg tatagtatta ggtgtattgg agattgctat
      241 acaatctatt attatgttcc ttgcagttag agaagcattt ctaggaatat tattagttgg
      301 aaatgtaact acttttataa ggtctttatc tacaatgcaa acaagtacac aaaatatagt
      361 aaataatatc tattctttct ataatggttc attgtacatg gagttattgt atgattttac
      421 gcatgatgag ttagtacata accctataat cgaaagtacg gttgatacaa ttcagtcagt
      481 tgaatttgaa agagtttctt tttcatataa tggtaaggat aatgtaataa acgatataag
      541 tttttcaatt aaagcaggag agaggttagc aattgttggt gaaaatggtt ctagaaaaag
      601 tacgattttc aaattagcct gcggtcttta cgataattat gaaggcaata tatatataaa
      661 tggtattaat cttcgttcaa tacagaaaaa gtcatactac aagagaatat ccgcattgtt
      721 ccaagatttt ttaaagtatg aattgacact gcgtgaaaat gttggattgg gtgaattatc
      781 aaagttatat agtgatgagg atttaataag tgcactgaag tatacaggag ttgattcgat
      841 attttacttt aatcaggaaa aattaaatca gatagatctg gaacagcaat tagggaattg
      901 gtttgaggat gggagacagt tatctggtgg acagtggcaa aaaattgcat tatcaagagt
      961 atatctcaaa gaagcggatt gctatttgct agatgagcct agttctgcgt tagatcctga
     1021 gtctgaaaca aaaattttta aaacattttt tgaattatcg caaaataaaa ttggtatttt
     1081 tattacgcat aagccaagca ttactcaatc tgtgaataaa attttgtatc tagataatgg
     1141 atgtatatct gaaacaggaa gtttcgctga tttaaatatt aaaggaatga agtttaaaaa
     1201 cttattagat aaagaaattg gagccacata tgacacctca aattaaacag atagtagatt
     1261 acattgcaga tagttttata aattatgaac agatagtagc tgaaagtgag aataaagtac
     1321 taattaatat tgaatctaaa tatagaaata ccttttttga taaatctctg agtcatggtg
     1381 tgccatcatt aatcttaatg tatagttcac tatataaagt gacaaaagat gagagatatc
     1441 taacattctc caacatatac gttgagaaat tagtagacat aatctctaga gatggtatag
     1501 aatctccgtc tctctatgct ggcactgcag gtattgcatt agctattaaa gaagcgagta
     1561 tatctggaaa gtattatccg aaattattat catccttaaa ttgtcttcta aaagaacaaa
     1621 ttaaagacaa attggtagta agtttttcta atattgagaa aggaattatt gagccatatg
     1681 attatgatat ggttaacgga tttagtggaa taactaatta cttactcctt gaacaagaat
     1741 ttttttctgt agagttaaat caaattggaa attatctttt gaaatatatt gagacaattt
     1801 taaataaggt aactaattgt tctgaagata gagaagctga atttgatcta ggaattgcac
     1861 atggaattac tggtcctatg ctaatcctag caaaattaaa atcagaaaaa attttgagtg
     1921 tagaagtagg agatatactg aataaagcta taaatttaat gtttttattt agaagggatg
     1981 acaaactatg gcctggaaaa atttatacta gtaatatttt ggatcttgat ttaaattatc
     2041 ttcctactag aatggcatgg tgttacggta tgcctggaac tgcagttgcc ctttttaaaa
     2101 tgtgtcaatt gatagattta aattttattg acgatattaa tgaaagttta attgctcaga
     2161 tgagaagttc agacaaaaat gtctattctc ttacaatgtg ccatggtttg gcaggagtaa
     2221 cttttatata tgacttgatt ggtaaaaatt attcaagtga gaggctaact cgctttgcag
     2281 aagagcttcg ccaacaaata attacatcgt tttcaactac aaaagtattt gggtatactg
     2341 acagagaaaa atttggagaa atagaagttg aactggagtc aatagggata ttgcaagggg
     2401 tatcagggat agtgttattc ttattaaatc cttacagtga cgaggagcta ttgtggcaaa
     2461 gaatatttat ttaatttgaa aaggagaaca aaatgtataa ggtatttcca actggtatga
     2521 ttcgtattcc attgcaaaaa caagaatttt ataataaaga ctatgaagat ttaataaatg
     2581 ataaagtttt tatggaacaa cttttagttg ctagtcccag tctttatgat agtgtaatag
     2641 attttgataa atgtaatgaa aagaaaaagc aaaatgtaaa agtctcatta aatagttatt
     2701 ctaaaagagc agcgtttaga actactccat ttggtatctt tactgctatt aatacagttg
     2761 atttgacaaa gggaaccact tctaatgtac aaaaggttag ttttattaaa aaagcagtcc
     2821 cagattattc atggatatat tctctagtaa aatcgtatga aataagtaac cttgaaaaac
     2881 tttcattaaa aataaataca gcggcgttta ctcaaggtga cagatatgta ctaccattta
     2941 cagtgaatga atcagaggaa gatagaaaca taagtttttc aaaaccaatt aagttacttg
     3001 tagaaaaatg taaaactcga tatataaaat atgaagagtt aatagatgta tttaaaaata
     3061 attatccaga tatagtgtca gacatgctag agtcatatat acacgactta gttacaaatg
     3121 attttttaat ttcagattta agaccaccaa tttgtaatat caattcctta gattatctac
     3181 tatctaaatt agaagaagga acactatgta ctgacttaac tactttaaag aagatgattg
     3241 aagattacaa cgatttaaat attggggaag gtagagactt acttttgcag atttttgata
     3301 aaatggaaag tatttataaa tatgataacg gtggaaatta cttaaaagtt gattcattag
     3361 cagagtatga aggcgatttt aatattctat ctgaaacggc tcgggaaagt atagaacgac
     3421 tcgcagagat gtttgtaaaa ttaaatacaa atgtaaagag aagagggggg aggaaaaatt
     3481 cacttgaaga gtatcagttg aaatttattg gcaaatatgg agaaaactgc tcaattccct
     3541 ttgttgaagt aataaataaa gatgcaggca taggttttcc tgaatattac aaaggaggag
     3601 aaggtgaagc aatagagtta agcgatccaa taatgcaaat gtttgaaaag aaatatgagg
     3661 aagctctatt aaatggaggg catatcgaat tttattcaaa agatttagat gattttcaaa
     3721 cagatcaaag caatagttta gattcgtttg aattaaattt taatataaaa ataataaata
     3781 atgatgtgaa gttatattta ggagctaata tcggttctgg acaagcaggt agaagctttg
     3841 gaagattcta tggattaagc gaaacagtaa gagaaacaat taagaattta aaccatcaaa
     3901 ataatacgaa tgtagagttg tcatttgttc ctaaacagat aagacttgcg aatgtaattc
     3961 aaaattatag tgatgagtct tataatactt cagtttttac cacatcatgg gattcagaga
     4021 acgaactgcg attggaggat atttatataa gatatagtga tggtaagttt cattttacaa
     4081 ctatcgatgg ggaaaatgag ttgaaattta ccatgaataa tatgctaaat tccgatagcc
     4141 aaagcagggt tttgagattg ttggttgatt tatctgagtt tgagtacggt ttatcgcatt
     4201 ggtcattatt tccttgggat attttggctc aggaaagagt atatattcct gaaattttat
     4261 ttgaagacat tccgattgcc actgctcagt ggaatttatc agtaattaga gaagagatta
     4321 tacatcaaaa aatattt
//
LOCUS       DAUCWO010000089         4309 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUCWO010000089 DAUCWO010000000
VERSION     DAUCWO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.089"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..266
                     /locus_tag="VGY20_001600"
     CDS             <1..266
                     /locus_tag="VGY20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEQ4274425.1"
                     /translation="ELQVQKDLKLRKENRIQSIHSSLAIEQNSLTVEQITAIIDGKRV
                     LGNPREIREVKNAYEAYEEILTLTPYDESHFLKMKEFQQYIYR"
     gene            290..1114
                     /locus_tag="VGY20_001601"
     CDS             290..1114
                     /locus_tag="VGY20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine ligase"
                     /protein_id="HEQ4274426.1"
                     /translation="MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYL
                     MPTFRGEAVDHRHLSQFYHSECEIEGDLHDVMNLVDRYIKFLVSEILRECRDSIFAIT
                     KDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
                     VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQ
                     VDASEYSWYLEMKKTRPMKSSGFGLGVERLIAWILKCDDVRKVQLISRENGIDYLP"
     gene            1128..2372
                     /locus_tag="VGY20_001602"
                     /pseudo
     CDS             1128..2372
                     /locus_tag="VGY20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
     gene            2297..3316
                     /locus_tag="VGY20_001603"
     CDS             2297..3316
                     /locus_tag="VGY20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEQ4274427.1"
                     /translation="MFGKQGIRCARKGDERNIKIIVNRVNSERNIKSIGICSPSYPGT
                     FHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGEISGGVQDRAEELNQLIPRVDVIM
                     TSIGGYNSNSILKYIDYDLFKQKSPIFIGYSDTTALALALYNKTGCITYLSQSVISNF
                     GEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFN
                     KGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGI
                     FDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKI
                     TLSLS"
     gene            3329..3529
                     /locus_tag="VGY20_001604"
     CDS             3329..3529
                     /locus_tag="VGY20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274428.1"
                     /translation="MNNRESKLTGFYDVFEEEVEKINHIHNVFVEESEKFGFQQMKIA
                     MVEHRKKYLDATDVHYTKYKNI"
     gene            3649..4134
                     /locus_tag="VGY20_001605"
     CDS             3649..4134
                     /locus_tag="VGY20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274429.1"
                     /translation="MLFRDRIPTYKGYRRQFNQILLGAWGIESAYVDAEIIVATWRGL
                     QRFKGIKVEFIQLSNKNIFDVLEKDLSKKLRFEDISIEAILGKYLCNNDIEIIKCLYE
                     KDKINMELLISLISKISNKLVKQEFIKVLVLYEYVKTFYQQIAFTFHYLIYMEQDIIQ
                     V"
     gene            4131..>4309
                     /locus_tag="VGY20_001606"
     CDS             4131..>4309
                     /locus_tag="VGY20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274430.1"
                     /translation="MNYKIFIRTKSGDIFDIADGGRIDDMVSKFNKVNVLGVCMGIGT
                     TVLSQEIEYEIEDRI"
BASE COUNT         1616 a          502 c          840 g         1351 t
ORIGIN      
        1 tagaattaca agtacagaaa gatttaaaat taagaaaaga aaatcgtatt cagtctattc
       61 actcatcact cgctattgaa caaaattctt taacagtaga acaaataaca gccattattg
      121 atgggaaaag agtgttagga aacccccgtg agataagaga agtaaaaaac gcttatgaag
      181 cttatgaaga aattttaacg ctaactccct atgatgaatc acatttttta aaaatgaagg
      241 aatttcaaca gtacatttac cggtaacaac atcgtcaatc acaagtccta tggggttggg
      301 gagtgattcg ctacctgtaa aaatttcctt atttgatgtt gatacctatc ttgcagattc
      361 gatgcagttt cacttagagt atatacttag actttttcca aagtcaggag ttcattactt
      421 aatgcctact tttagaggag aagcagtaga tcatcgacat ttatcgcaat tttatcatag
      481 tgagtgtgag atagagggag atttacacga tgtgatgaat ttagtggata gatatattaa
      541 gtttctggtt agtgaaattc ttagagagtg tcgagatagt atttttgcta taacaaaaga
      601 tgtaagtcat ttggaaagtt ttattgatcg aaagatacca attattacgt ttgaagaggc
      661 ttgtactata ttggaaaatg aaatagattc attggaatat aatgatgaac atcattttta
      721 tgtgttaaca gatgttgggg aaaagaaatt aatggaacat tttgagggag tagtttgggt
      781 aaaaaattat ccctgtaaaa gtgtcccttt ttatcaaagg ttctcaacag atcatctatt
      841 tgcctataat gcagatttgt tgttaggaat aggcgaaact gtaggttgtg gagaaagatg
      901 cgaaacatat gaagaagttg tagaaaatat gaattattta caagtagatg cttctgaata
      961 tagttggtat ttagaaatga aaaaaacaag acctatgaaa tcttcaggat ttggtttagg
     1021 agtagagcgt ttaatagcgt ggatattgaa gtgcgatgat gttcgaaaag ttcaattaat
     1081 cagtagagaa aatgggattg attatttacc ataaaggagg aagaaaaatg aaaaagaata
     1141 ttttaattgt aggtggaggt ccagagataa ctatatgtta tgatttaatt aacaatagta
     1201 cggagtataa tttcttttca gtatcagaaa ttcctccgat gtatccagag ttgtttgtag
     1261 aaacaattga aaccagcttt agaaattttg gaaagacgat tgaacaaatt aaagagctca
     1321 aatattctat tgatgctgtt gcttgttggg atgaagcttt aacgcatata gctgatgata
     1381 tttcaaaaga attaggattg aaccctattt catcgctaga ttctcaatca ttcaggttta
     1441 aagatcgaat gagaatggta tgtgaagctg gtggactaaa aatgcccaaa tataaaatta
     1501 tcaatcaatt ttcagataca aataaaataa tcaattggaa atatccatta atagtgaaac
     1561 caacctcttt tttagcaagt ataggagtga aaaaagttta taatttttca gaattacaac
     1621 aagcagtttc tcaaatgctt aatgtaaaat ttccggttta catagcttca ggggtatatg
     1681 aattggggaa ttatataatt tagaacctag agttttggtg gaagagttta ttgatggaga
     1741 agaatatagc ttagagagcg tggtaagaaa tggaatctat acacctctag ggataactaa
     1801 aaaatagtgg atgaaaaatt atttatggat gaaataggcc atattttccc ttccaattta
     1861 aacaaagaag aaaaaagtag agtatattct tgggcagaaa aattacatca aattttgcaa
     1921 ttaaatcata ttacgactca tacagaattt agaattggaa gaaatggaga tattatttta
     1981 atagaaattg gagcaagaat cggtggagat tgtatccaaa cttaatgaaa aacagcttta
     2041 tagtcaaggg atttgatttt tataatactt atttaaaagc tagactaggt ttacaagaag
     2101 agttaaatac agacactaat aagttttcag gaattgtatt tttcagactc aatccagaga
     2161 attacggaaa aatatttaat ggagtgagtt acgatgcacc taaaacttca ctaatatttg
     2221 aagaaaagat gtataaagag gaaggaactg tattgccaaa tccgattaat tggagtaatg
     2281 agagagtagg atatgtttgt ttggaaaaca aggaatacgc tgtgctagaa aaggagatga
     2341 aagaaatatt aaaatcattg tcaatagagt aaatagtgaa agaaacatca aatctatagg
     2401 tatttgttca ccctcctatc cggggacatt tcattatcag aaacgatata agagaggagt
     2461 agaattttta caaaataaag gatttactat agtagagggg aatttaacag gaaaagaagg
     2521 ttatagaggg gaaatttcag gaggtgttca agacagagct gaagaattaa atcaacttat
     2581 tccgcgtgtg gatgtcatca tgacttccat aggtggctac aattctaatt ctatattaaa
     2641 gtatatagac tacgatctat ttaaacagaa atctcctatc tttattggat attctgacac
     2701 aacagctctt gctttagcct tatataataa gactggatgt ataacttacc tgtctcagag
     2761 cgttatctct aattttggag agtttgaacc gtttaatgaa ttaaattatt tctattttga
     2821 cttcatgctt cagagtaagt gcgaaacctt aatggtacaa ataccagatg tttggacgga
     2881 tgagtggata aattgggaaa cctatgaaag aaccaaaaaa acaaataaaa atgagtggat
     2941 tatttttaac aaaggtgaat ttaacggtac tttgatcgga ggaaatctcg ataccattgt
     3001 aggaataata ggaactgagt atatgccaaa aataacagag gataccatac tcttgttaga
     3061 agatgtatat acggatctag gaagattata taggaatttc acaacgcttg ccttacatgg
     3121 tatttttgat aaaataggag gacttattat ttcaaaattt gaaacaatag gggaaaatag
     3181 cgatgttatt aatgatatca ttaacgaatt tgtagggcat agaaagattc ctattttatt
     3241 gaattttgat tgtggtcata cccatcctag ctgtttaatg cctattggtg gtaaaattac
     3301 gttgagttta agctaaaaga ggtggtatat gaacaataga gaaagtaaat taacgggttt
     3361 ctatgatgtt tttgaggaag aagtagaaaa aataaatcat attcataatg tttttgtaga
     3421 ggagtcagaa aaatttggct ttcaacagat gaagattgct atggtagagc atagaaaaaa
     3481 atacttagat gcaaccgatg ttcattatac aaaatacaaa aatatttgaa gttcatagag
     3541 taaaagaaga gtcagtatac gccctccaat cagatttggc acttagtatg tcgccgttgt
     3601 atagctaact taccacaaca aatacctgta aagtggattc aagacagagt gttatttaga
     3661 gatagaattc ctacatataa aggttataga agacaattta atcaaatatt attaggtgca
     3721 tggggaatag aatcagctta tgtggatgca gaaataatag ttgcaacttg gcgaggcttg
     3781 caacgattta aaggaattaa agtagaattt attcaactgt ctaacaaaaa tatatttgat
     3841 gttttggaga aagatttatc taaaaagtta agatttgaag atatttcaat tgaagcaata
     3901 ttgggtaaat atttgtgtaa taatgacata gaaatcataa aatgtcttta tgaaaaagac
     3961 aaaataaata tggaactctt aattagtctt atttcaaaaa taagtaacaa actggtgaaa
     4021 caggaattta taaaagtttt agttctgtac gagtatgtaa aaacttttta ccagcagatt
     4081 gcatttactt ttcattatct aatttatatg gaacaggaca ttattcaagt atgaactata
     4141 aaatcttcat acgaactaag tcaggtgaca tatttgatat tgctgatggt ggtagaattg
     4201 atgatatggt ttctaaattt aataaagtaa acgtattagg agtttgcatg ggaataggaa
     4261 ccactgtttt atcacaagag attgagtatg aaattgaaga tagaataat
//
LOCUS       DAUCWO010000090         4282 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUCWO010000090 DAUCWO010000000
VERSION     DAUCWO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.090"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            178..1443
                     /gene="purD"
                     /locus_tag="VGY20_001607"
     CDS             178..1443
                     /gene="purD"
                     /locus_tag="VGY20_001607"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ4274431.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTGAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQNDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            1721..2212
                     /gene="purE"
                     /locus_tag="VGY20_001608"
     CDS             1721..2212
                     /gene="purE"
                     /locus_tag="VGY20_001608"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ4274432.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            2199..3272
                     /gene="purK"
                     /locus_tag="VGY20_001609"
     CDS             2199..3272
                     /gene="purK"
                     /locus_tag="VGY20_001609"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ4274433.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPTNDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVMTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTLFAEDADEVEEF"
     gene            3293..4282
                     /locus_tag="VGY20_001610"
                     /pseudo
     CDS             3293..4282
                     /locus_tag="VGY20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1227 a          837 c         1030 g         1188 t
ORIGIN      
        1 ggctttactt gatattaaat aactgtttga ttggctaata gagtgttcct tatttcaaga
       61 attgtggtct actatcacat ttttacaatg tttcaattat tgataattaa tacattgtat
      121 aaaaaggcga acgtttatga tataatatct aattataata cgaaagaggt ggatgttttg
      181 aaattacttg ttgttggttc aggtggtcgt gaacatgcga ttgctaagaa gttgttagcg
      241 tctaagggtg tggatcaggt ttttgtggca cctggtaatg atggtatgac cttggatggt
      301 ctggacttgg tgaatatagt agtttccgaa cattccaggt tgattgcctt tgctaaggag
      361 aatgagattt cttgggcctt tattggtcct gatgatgcgc tagcagctgg tatcgttgat
      421 gatttcaaca gtgctggact cagagctttt ggtccaacca aggcagcagc ggagctagag
      481 tggtcaaaag actttgccaa ggaaatcatg gtcaaataca atgtcccaac aggagcctat
      541 ggcacatttt cagactttga aaaagccaaa gcctacatcg aagagcaggg cgcacctatc
      601 gtggtcaagg ctgatggctt ggcgctaggt aagggtgtgg tcgtggctga aaccgttgag
      661 caggcggtag aggcggcgca agagatgctt ctggacaata agtttggcga ttcaggagcg
      721 cgtgtggtta tcgaggaatt cttggatggc gaggagtttt ccctctttgc ctttgttaat
      781 ggtgataagt tctacatcat gccgacagct caggatcaca agcgtgcctt tgacggagac
      841 aaggggccaa acactggtgg tatgggtgcc tatgcgccag ttcctcatct acctcagagc
      901 gtggtggata cagcggttga gacgatcgtt aggcctgtcc ttgagggtat gattgccgaa
      961 gggcgtcctt atctaggcgt cctctatgcg gggcttatcc tgacggctga tggtcctaag
     1021 gttatcgagt tcaactcacg tttcggtgac cctgaaactc agattatcct ccctcgcctg
     1081 acttccgatt tcgctcagaa tattgacgat atcatgatgg gcattgagcc ttacatcact
     1141 tggcagaatg acggtgtgac tttgggtgtt gtcgttgcct cagaaggcta tccgctcgat
     1201 tacgagaaag gtgtgccact gcctgaaaag accgacggcg atatcatcac ctactatgcc
     1261 ggagctaagt ttgcggaaaa tagcgagcta cttctctcaa acggaggacg tgtctatatg
     1321 ctcgtcacca cagaagacag cgtcaaagca gggcaggaca aaatctatac ccaactggcc
     1381 caacaagaca caacaggtct cttctaccga aatgacatcg gaagcaaagc tataagagaa
     1441 taagactaat cattcaaata ttagcgtcag caatcagcga ccgaaggtcg ctccaaaaag
     1501 aaagaagaaa ggaactgaac acgggactaa tttcctagga aaaaaataaa actacctgga
     1561 cgcaagcgtc atcgttagtt ttctgcgtcg taggcgtctg atgctaaagc acctagtcgc
     1621 cctgacgtgg gttcgtcttc gaaatcaaag atttcggact taccgcatat ttttctacgg
     1681 aaagtttcga caagtcgaaa cgccccttta tatcttaatt atgaaaactc caattatttc
     1741 tattatcatg ggctccaaat ctgactgggc aaccatgcaa aaaacggctg aagttctaga
     1801 caactttggc attgcttacg agaagaaagt cgtctctgct caccgcacgc ccgacctcat
     1861 gttcaagcat gctgaggaag cacgtggtcg tggcatcaaa atcatcatcg caggagcagg
     1921 tggggcagct cacttgcctg gtatggtggc agctaaaaca acgctgcctg ttatcggtgt
     1981 gcctgtcaaa tcacgtgccc tttcaggttt ggattcactc tattccattg tgcagatgcc
     2041 tggcggtgtg cctgtggcaa ccatggctat cggagaagcg ggagcgacta acgctgccct
     2101 gacagccctc cgtatccttt ctatcgagga ccaaaatctg gctgatgcgc tagctcattt
     2161 ccatgaggaa caaggaaaaa tcgcagagga gtcaagtggt gaactcattt aagaccattg
     2221 ggattattgg tggtggtcag ctagggcaga tgatggccat tgcggccatc tatatgggcc
     2281 acaaggtcat cacgctggat cctactaacg actgccctgc ctcccgcgtt agcgaggtaa
     2341 ttgtggcgcc ttacgatgat gttgaggctt tgggacaatt ggctgcgcgt tgcgatgtgt
     2401 tgacttatga gtttgagaat gttgatgcgg acggtttgga tgccgttgtg tcagcttgtc
     2461 agctaccgca ggggaccgat ctgctccgca tttcccaaaa ccgtatcgtt gaaaaagact
     2521 ttctggcaaa taaggctggt gtgacagtcg ctccctataa ggtgatgacg tctagccttg
     2581 acctaggggg gcttgatttg accaagacct atgtcctcaa gacagcgaca ggtggttatg
     2641 acggtcatgg gcaaaagatt atccgctcag cagaagattt gccagaggcg cagcaattag
     2701 ctaactcagc tcagtgtgtc ttggaagagt ttgtcaattt cgaccttgaa atctcagtta
     2761 tcgtgtctgg aaacggagag gatgtgacgg tctttcccgt tcaggaaaat atccactgca
     2821 ataatatcct gtcaaaaact atcgtgccag ctcgcatctc agatcaactg gctgataagg
     2881 ctaagaaaat ggcggtgcag attgccaaga aactccagct gtcaggaact ctctgtgtgg
     2941 aaatgtttgc gaccgcagat gatatcattg tcaatgaaat tgccccacgt ccccacaact
     3001 cagggcacta ctcaatcgag gcttgcgact tttcacagtt tgacacccac attttgggcg
     3061 tgctaggagc gccgctccca accatccacc tccatgctcc agccgtcatg cttaatgtcc
     3121 tcggacaaca cgtccagcag gcaattgact atgtgactga aaaccctagc gcccacctcc
     3181 acatgtatgg taaactagaa gcgaaacata accgcaaaat gggacatgtg acactatttg
     3241 cagaagatgc ggatgaggtg gaggagtttt agaacaggag tagattggta agatggagtt
     3301 aagggaattt aaagcgtggt tattaaaaaa ccattttgaa gaagatgagt tatacaagga
     3361 atctataaag tgttatcaag tggaagcata taaggctgcc tatatttttt cgtatttagc
     3421 aaatcataag ttcattgcta aattagctat tgactacagt ggtgtcccat caaattttat
     3481 gtctaaattt cccgatgatg aagttagaaa gtcgagttgg caaaaacgtg tgaattcctt
     3541 aaaaaatgaa gatgagtggg agcagacggt taacgaaaaa ttaattcaag catgggaaga
     3601 tgataatgta ttcaaattac ctttcaaaat taaagaaaaa tattctcata tgagggtatt
     3661 gcgtaataat gcagcccatg cgaaagaccg tcgaatttct gaatcaacag ttttagaatt
     3721 atggaatgaa attgagtata tatatcctta ctttgtaatc aacggaacga ttgatgcatg
     3781 gatggaagat ttagaaaaac tagttagata ttcaaatagt agcatagaat ctcaaaacca
     3841 attacagaat aaatttggag atttttctac tgaaaataag accttaattg ttaaaaagat
     3901 tatacaaaaa tatttggtag attctgaatg ggatgaaacg tacccagata tagtgatgaa
     3961 ttttttatta tatatttaat tatattaatc aaattggtaa agacttaacg gaaaaagagg
     4021 aattgtatct gtctattgga acaggtattt atcatttcca agctgaagct attgatattt
     4081 tttctcaatt tgaagagtct ctgagtgatt atacatttag atatttttgt gaagaatttt
     4141 cggataattt ttggaagttt attgattcta tggattccga agttaataaa gagaacttga
     4201 cagctactag tttagaaatt ttgaagaaaa attctgctat tttagtaaat gcagaattta
     4261 atgagagccg atttttatct ga
//
LOCUS       DAUCWO010000091         4268 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUCWO010000091 DAUCWO010000000
VERSION     DAUCWO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.091"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            291..554
                     /locus_tag="VGY20_001611"
     CDS             291..554
                     /locus_tag="VGY20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ4274434.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            559..2292
                     /gene="ptsP"
                     /locus_tag="VGY20_001612"
     CDS             559..2292
                     /gene="ptsP"
                     /locus_tag="VGY20_001612"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ4274435.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWVGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            2477..3904
                     /locus_tag="VGY20_001613"
     CDS             2477..3904
                     /locus_tag="VGY20_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ4274436.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEADADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
     gene            3999..>4268
                     /locus_tag="VGY20_001614"
     CDS             3999..>4268
                     /locus_tag="VGY20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEQ4274437.1"
                     /translation="MKKLNVILVGLLSILMLSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSDLVIKAVKHIKKDQKDYYYFSPIKQADDFFVDNLPVSLYKKKNS"
BASE COUNT         1346 a          757 c          920 g         1245 t
ORIGIN      
        1 cacaacatat tgtgttgaaa taaaaaacag aaacctaaat attgtgtcaa tctggattct
       61 tatggttaga gctctagcgt tttggtgata gaagctaagc agttttaata aagcattaaa
      121 gtctgaaaaa taaattgtga aaaacgtgaa aataaaagaa aataatttca cattttttgt
      181 cgtttaaata tttaatcttg aaaataaaaa agcattgtgc tataatcggt tgttgtaagg
      241 gttatcatgt gatggctcaa aaatacacaa cttaaaagga gaaccatact atggcttcaa
      301 aagactttca cattgttgca gaaacaggta ttcatgcgcg tccagcgact ttgcttgttc
      361 aaacagctag caaatttgct tcagacatca ctcttgacta caaaggtaaa gcagtaaatc
      421 taaaatcaat catgggtgtt atgagtcttg gtgttggtca aggtgcagat gttactatct
      481 cagctgaagg tgcagatgct gaagatgcca ttgcagcaat tgaagaaaca atgactaaag
      541 agggattggc ataagactat gacagaaatg cttaaaggaa ttgcagcctc agacggcgtt
      601 gctgttgcta aagcatatct actagttcag ccggatttgt catttgagac tgttacagtc
      661 gcagatacaa atgcagaaga agctcgcctt gatgttgcac tccaagctgc acaagacgag
      721 ctttctgtta tccgtgaaaa tgcagtagaa agcttaggtg aagaagcagc agccgttttt
      781 gatgcccatt taatggttct tgctgatcca gaaatgatca gccaggttaa agaaacgatt
      841 cgcgcaaaac aaacgaatgc agaaacaggt cttaaagaag tgactgacat gttcatcacc
      901 atctttgaag gcatggaaga taacccatac atgcaagaac gtgcagcgga catccgcgac
      961 gttgcaaagc gtgtgttggc tcacctttta ggtgtaaaac ttccaaatcc agctacaatc
     1021 aatgaagaat caatcgttat cgcacacgat ttgacacctt cagatactgc tcaacttaac
     1081 aaacaatttg taaaagcatt tgttacaaat atcggtggtc gtacaagtca ctcagctatc
     1141 atggcacgta cacttgagat cgctgcggta cttggaacaa atgatattac aaaacgtgtt
     1201 aaagatggtg atgtgattgc cgttaatggt atcactggtg aagtgattat cgatccaagc
     1261 gaagatcaag tacttgcttt taaagaagct ggtgcggctt atgccaaaca aaaagcagag
     1321 tggtctctcc ttaaagatgc gcatactgag acagctgatg gcaaacactt tgaattggct
     1381 gctaatatcg gtacgcctaa agacgttgaa ggtgttaatg acaatggcgc tgaagctgtt
     1441 ggcctttacc gtactgagtt cttgtacatg gattctcaag acttcccaac tgaagacgaa
     1501 caatacgaag cttacaaggc agtgcttgaa ggcatgaatg gcaaacctgt tgtggttcgt
     1561 acgatggata ttggtggcga caaggaactt ccttactttg accttccaaa agaaatgaac
     1621 ccattcctcg gtttccgtgc tcttcgtatc tcaatctctg aaactgggga tgccatgttc
     1681 cgcacacaaa tgcgtgcgct tcttcgcgcc tctgttcacg gacaacttcg tatcatgttc
     1741 ccaatggtag cacttcttaa agaattccgt gctgcaaaag cagtctttga cgaagaaaaa
     1801 gcaaacttgc ttgcagaagg cgttgcggtt gctgatgaca tccaagttgg tatcatgatt
     1861 gagattcctg cagctgctat gcttgcagac caatttgcta aggaagttga tttcttctca
     1921 attggaacaa acgaccttat ccaatacact atggcagcag accgtatgaa cgaacaagta
     1981 tcataccttt accaaccata caacccatca atattacgtt tgatcaacaa tgtgatcaaa
     2041 gcagcgcacg ctgaaggtaa atgggtaggt atgtgtggtg agatggcagg tgaccaacaa
     2101 gctgttccac ttcttgttgg aatgggcttg gatgagtttt ctatgtcagc aacttcagta
     2161 cttcgtacgc gtagtttaat gaagaaactt gactctgcta agatggaaga atatgcaaat
     2221 cgtgcgctta cagaatgttc aacagcagaa gaagttcttg aactttctaa agaatacgtt
     2281 tctgaagatt aacgggttaa agagttggtt taaaccagct cttttttgct agctaaaaat
     2341 acaattactt tttggtgtga aatcatttga gttatcaaat aattatcaga attttcagat
     2401 atttagtgta gccaaatctt gaattatatc ggaaaagtga tagaataaaa ttactatcat
     2461 aaaaggagat aaaacgttgg caaaacaata taaaaattta gtgaacggtg aatggaaact
     2521 atcagaaaac gagattacca tttacgcacc agcaacaggt gaagagttag gatcagttcc
     2581 agcgatgacg caggcagagg tagatgctgt ttacgcttca gctaaaaagg ctctatcaga
     2641 ttggcgcgcc ttgtcttatg tggaacgtgc agcttacctt cataaagcgg ctgatatttt
     2701 agtacgtgat gctgaaaaga tcggcgcgat tctttcaaaa gaagtagcca aaggtcacaa
     2761 ggcagctgtc agtgaagtta ttcgtaccgc tgaaatcatt aattatgcag cagaagaagg
     2821 gcttcgtatg gaaggtgaag ttcttgaagg tggtagcttc gaagccgcaa gtaagaagaa
     2881 gattgctatt gttcgtcgtg aaccagttgg tttagttctt gccatctcac cttttaatta
     2941 tcccgttaac ttggcaggtt ctaaaattgc tccagccctt atttcaggaa atgttgttgc
     3001 tcttaaacca ccaacacaag gctctatttc tggtttgtta ctagcagaag cttttgcaga
     3061 agctggtatt ccagcaggtg tctttaatac cattacaggg cgaggttctg ttattgggga
     3121 ttacatcgtt gagcatgaag cggttaactt tatcaacttt acaggttcta ctccaattgg
     3181 ggaaggaatc ggtaaattag cgggtatgcg accaattatg cttgagcttg gcggtaagga
     3241 ttctgctatc gttttggaag atgcagatct tgttttagca gcgaaaaata ttgtagccgg
     3301 tgcttttggt tactcaggac aacgttgtac agcggttaaa cgcgttcttg tgatggacaa
     3361 ggtggcggat caattggcgg ctgagattaa aacacttgtt gaaaaactaa gtgtcggaat
     3421 gcctgaagcc gatgctgata ttacaccatt aattgataca tcagctgctg attttgttga
     3481 agggttgatt aaagatgcaa ctgataaggg agctactgct ttgacagcct ttaatcgtga
     3541 aggcaatctt atttcacccg ttctctttga tcatgtgaca actgacatgc gtttggcatg
     3601 ggaagagccg ttcggcccag tattaccaat tattcgtgta accactgtag aagaagccat
     3661 caagatttct aatgagtctg aatatggttt gcaagcttct atttttacaa ctaatttccc
     3721 aaaagctttt ggcattgctg agcaattaga agttggaact gttcacctta acaataaaac
     3781 acaacgtgga acagataatt tcccattctt aggcgctaaa aaatcaggtg caggggtaca
     3841 aggagttaaa tattctatcg aagctatgac aactgttaaa tctgttgtat ttgatatcca
     3901 gtaataaaaa aaataaacaa aaagttaggt taacttaact ttttgtttat ttttttaatg
     3961 ttttttgtta caatagtcta tacagatgga ggcaactatt gaaaaaatta aatgttattc
     4021 ttgttggttt attaagcatt ctgatgttga gtttagctat tgtgtttatt aatcgttgga
     4081 aactaaacga agatagtcag cgtatagttt tggctgaaaa gaaaaaaaac acgtcagatt
     4141 tagtgatcaa agctgtaaaa catattaaaa aagatcaaaa agactattat tatttttccc
     4201 cgataaaaca agcagatgat ttttttgtag ataatttacc tgtttcatta tacaaaaaaa
     4261 agaattca
//
LOCUS       DAUCWO010000092         4207 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUCWO010000092 DAUCWO010000000
VERSION     DAUCWO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.092"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            78..2597
                     /gene="secA"
                     /locus_tag="VGY20_001615"
     CDS             78..2597
                     /gene="secA"
                     /locus_tag="VGY20_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ4274438.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            2713..3069
                     /locus_tag="VGY20_001616"
     CDS             2713..3069
                     /locus_tag="VGY20_001616"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ4274439.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            3066..4166
                     /locus_tag="VGY20_001617"
     CDS             3066..4166
                     /locus_tag="VGY20_001617"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ4274440.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
BASE COUNT         1302 a          736 c          936 g         1233 t
ORIGIN      
        1 taactttacc aacctttctt gaactgcctg catatttatg ttaaaatgaa atgattagat
       61 gatcgaaagg aaagagaatg gccaatattc tacgcaaagt tatcgaaaat gacaaaggcg
      121 aactaagaaa attagaaaaa attgcaaaaa aagtagaatc ctacgcagac caaatggcgt
      181 cattgtcgga tagagatcta caaggaaaaa cacttgaatt taaagaacgc tatcaaaaag
      241 gtgagaccct cgaacaacta ttacctgaag ctttcgcagt tgttcgtgaa gcagcaaaac
      301 gtgtgttggg cttgttccct tatcgtgttc aaattatggg aggaattgtt cttcataacg
      361 gggatgtccc tgaaatgcgt actggtgaag gaaaaactct tacagccacc atgccagttt
      421 atctcaatgc tattgctggt gagggtgttc acgttattac tgttaatgag tatctttcaa
      481 cgcgtgacgc taccgaaatg ggtgaagtgt atagctggtt aggtctttca gttgggatca
      541 acctagctgc taagtctcct gctgaaaaac gtgaagctta taattgtgat attacttatt
      601 caaccaactc tgaagtcggt tttgattacc ttcgtgacaa catggttgtt cgccaggaag
      661 acatggtaca acgcccactc aattttgcct tggttgatga agtggactca gtcttaattg
      721 acgaggcaag gacaccttta attgtttctg gtgctgttag ctctgaaacc aatcaattgt
      781 atattcgtgc tgacatgttt gtaaaaactc tgacgtcagt ggattatgtt attgatgtgc
      841 ccactaaaac gattggatta agcgattcag gtattgataa agctgagagt tattttaact
      901 tgagcaatct ttacgatatt gaaaatgtag ccttgactca ctttatagac aacgcccttc
      961 gtgcaaacta tattatgttg ttagatatcg attatgtggt cagcgaagat ggtgagattc
     1021 ttattgttga ccaatttaca ggtcgtacga tggaagggcg tcgcttctca gacggcttgc
     1081 accaagctat tgaggcaaaa gaaggcgttc gtattcagga agaatcaaaa accagcgcct
     1141 caatcactta ccaaaatatg tttcgtatgt acaaaaaatt ggcaggtatg actggtactg
     1201 cgaagaccga ggaagaagaa tttcgcgaag tttataatat gcgtattatt ccaattccaa
     1261 ccaatcgtcc aattgcccgt attgatcaca ctgatttgct ttacccgacc ttagaatcga
     1321 agtttagagc agttgtggaa gacgttaaaa cgcgtcatgc aaaaggacaa cctatccttg
     1381 taggtacggt tgctgttgaa actagtgatt tgatttcacg caagctggtt gaagcaggga
     1441 ttcctcatga ggttttgaat gctaaaaatc actttaaaga agctcaaatc attatgaatg
     1501 ctgggcaacg tggtgctgtt acaatcgcaa ctaacatggc tggtcgtggt actgatatca
     1561 agcttggtga aggtgttcgt gaacttggtg ggttatgtgt cattggtaca gagcgtcatg
     1621 aaagtcgtcg tatcgataac cagcttcgtg ggcgttcagg tcgtcaagga gatccaggtg
     1681 aatcccaatt ctatctttca ttagaagatg atttgatgag acgtttcggc tctgatcgta
     1741 taaaagcctt tttggatcgg atgaaattag atgaagaaga cactgttatt aaatcaggta
     1801 tgttagggcg tcaagtggaa tctgctcaaa aacgggttga aggaaataac tacgataccc
     1861 gtaaacaggt cttacaatac gatgatgtga tgcgtgaaca gcgtgaaatt atctatgcta
     1921 accgtcgtga tgtcataact gctaatcgag atcttggccc agaaattaaa gctatgatca
     1981 agcgtactat cgaccgtgct gtagatgctc atgcacgtag caatcgtaaa gatgctatag
     2041 atgctattgt aacttttgct aggacctcgc tggtaccaga agagtctatt agtgctaaag
     2101 agttacgtgg tttaaaagat gatcaaatca aagagaagct ttatcaaaga gcgcttgcta
     2161 tctatgacca gcaactatca aaacttcgtg atcaagaagc tattattgaa ttccaaaaag
     2221 tactcatttt gatgattgtg gataacaaat ggactgaaca cattgatgcc ttagatcaac
     2281 ttcgtaatgc tgttgggctt cgggggtatg ctcaaaataa ccctgtggtg gaataccaag
     2341 cagaaggttt caagatgttc caagatatga ttggtgccat cgagtttgat gtaacacgta
     2401 ccatgatgaa agcacaaatt cacgaacaag aacgtgaacg tgcaagtcaa cgtgccacaa
     2461 cagcagcacc tcaaaacatc caatcgcagc agtcagctaa caccgatgat ttacctaaag
     2521 tggagcgtaa cgaagcttgt ccatgtggtt caggcaaaaa attcaaaaat tgtcacggtc
     2581 gtaaatcctt ctcataagaa cgagacacaa caggacaaag ttaggtatat agaaagaagc
     2641 tgagaataat tctcagcttc tcactgttta aatcttctaa cattaagatg aaataaggtt
     2701 tggtggaaag taatgattgt tggacatgga atagacttac aagagatttc agctattgaa
     2761 aaagtttatc aaagaaatcc tcgctttgct caaaaaattt taactgagca ggaattggct
     2821 atttttgaga gttttcctta caaacgtcga ctcagctatt tggcaggacg ctggtcaggt
     2881 aaggaagctt ttgctaaggc tatagggaca ggtattgggc ggttaacctt tcaagatatt
     2941 gagattttaa atgatgttag aggatgtccc attctgacaa aatctccgtt taaaggaaat
     3001 agttttatta gcatctcaca tagtggcaat tatgtacagg ctagtgttat tttggaggat
     3061 aaaaaatgat ttcaagtttc catcgcccaa cagttgcaag ggtcaattta caagctatta
     3121 aggagaatgt tgccagtgtt caaaagcata ttccactagg ggtaaaaacg tatgcagttg
     3181 tcaaggctga tgcttatggt catggtgctg tccaggtgtc aaaagcactc ctacctcaag
     3241 tggatgggta ctgtgtgtca aatcttgatg aggctttgca attacgtcaa gcaggtattg
     3301 ataaagagat tttaattctt ggggtcctcc tgccaaatga attagagtta gcagttgcta
     3361 atgctattac tgttacaatc gctagtttag actggatagc tttagctaga ctggagaaaa
     3421 aagaatgtca aggcttaaaa gttcatgtaa aagttgattc tggtatgggg cggatcgggc
     3481 ttcgttcttc aaaagaagtc aatttattga ttgatagtct aaaagagttg ggtgctgatg
     3541 tagaaggtat ttttactcat tttgccacag ctgatgaggc agatgatact aaatttaacc
     3601 agcagttaca gttttttaaa aagctgatag ctggacttga ggataagcct cgtttagtac
     3661 atgctagtaa ttcagccaca agtatctggc atagtgatac catttttaat gctgttcgtt
     3721 taggaattgt cagttatggt ttgaatccaa gtggttctga tctaagctta ccatttccac
     3781 tgcaagaggc tttatctcta gaatctagct tagtgcatgt caagatgatt tcagctggtg
     3841 atacagtcgg ttatggagct acttatactg ccaaaaagtc tgaatatgta gggaccgtcc
     3901 caatcggtta tgcagatggc tggaccagga acatgcaagg cttttcggtg ttagttgatg
     3961 gacaattctg tgaaattata gggcgtgtat cgatggatca actgaccata cgacttccca
     4021 aagcatatcc tttaggaaca aaagtcactt tgattggcag caatcagcaa aaaaatattt
     4081 ctacaacaga tatcgcaaat taccgtaata caatcaatta tgaagttcta tgccttttaa
     4141 gtgaccgtat tcctcggata tattaagaaa atcacgtaaa aataattgca ttgtgcttat
     4201 ctttaat
//
LOCUS       DAUCWO010000093         4142 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUCWO010000093 DAUCWO010000000
VERSION     DAUCWO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.093"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            458..1966
                     /locus_tag="VGY20_001618"
     CDS             458..1966
                     /locus_tag="VGY20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274441.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(2315..3664)
                     /locus_tag="VGY20_001619"
     CDS             complement(2315..3664)
                     /locus_tag="VGY20_001619"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ4274442.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
BASE COUNT         1244 a          813 c          724 g         1361 t
ORIGIN      
        1 aactagctct ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaac
       61 tttaaggtta ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg
      121 agaataggtt ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag
      181 gtgccctccc ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa
      241 agtcaacagc aaaaatatga tatcacccgc gtgttcttag ctctctcaat tatgggctaa
      301 aaacacgcat caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga
      361 ctatactggt aatcctattg attttagttg acttttacgg tagattcgct acaatatata
      421 ggattataca atttaattta aggagagaaa aattacattg ttagattatt atttggaatc
      481 cgatattatt gaccagaaaa tcctactggc tattttgttg acagaaaaag aactgacgat
      541 ggcagcagct tgttctcaaa caactttgac tgctttaaaa ataaagcagt atcttcgaca
      601 atttaacgcc ttatttaaag ggtacttacg tattgagctt attaaatctc gtatttattg
      661 tgaggtatta aatgataagc gagaagcatt tttttatgat atttttgcct tatcagatac
      721 tctaaaaatg ttgacattct tattactaga taatcccaaa cacaaatcaa tcgctgttta
      781 tacacgaaaa caagggatat cccagtcaaa agcttatcgt cttattcata aattaaaaca
      841 ttatttacaa gacattggtt taaatattgt agacaatact gttatcggag atgaacttaa
      901 aatacggtat ttgattgctc ttttacataa agagtacggc attatattat atgatataca
      961 acctgcagac attgaaacga ttcatgcttt tatctttgca gcacaaaaaa acttacaacc
     1021 ttctgccttt ttagatagac gcttcttgtt ttttgatgtg ctgttgatgt tgacttggaa
     1081 gcgccatcgc tatcctgttc atttgcctca tttagctctt tttgaacatc tcaaatcctt
     1141 gcctattttt gacaatatca aaaccattgc cgtggatgag ctagcacctc gaactcgtgt
     1201 cacattttcg tctgatgatt ttgactatct ctttttaatt tatttaacga ctgataactc
     1261 cttcttgagt gggtactgga caagtcatca gcgacaacaa ctttaccatt taatcaccaa
     1321 agatcccgat tatcatttac taatccatcg tcttcaggca ctagttggaa aatattccga
     1381 tattcatgag catatcccta acttaattcc ttttttcaaa agaactctct acaatttaca
     1441 aactttaatt acctttgatg gttattattt tgatcaatat caaggtaaca tgctactact
     1501 agataagctt gaaaccgtta tcaaagactg gctttatgac acaggccgtc aaggcagtat
     1561 tagttctggt cacttacact tgatgtgttt ataccttgag cagatcttgg aaagctctat
     1621 tgctccaatc aatattactg ttattgagag ccaagaaact gttggtaacg ttatagctaa
     1681 tttcatcacc tcaactatcc cttcttacaa agttgaactg tcacgggtca atatcttatc
     1741 tgataatatt tatccctatg ataaacctgt agatctggtt gtaaccagtc agaagctctt
     1801 gcctttctta aaagaactcg gtgtttttcc caaagagacc cgcttgtttg gtttgagttt
     1861 ggactgtatc caacagcagc gcgaagattt aatcaaaacc atcttagcct tgcaccagaa
     1921 ccactaccaa aaacgtttag aagaactttg gagagttcct tcttaatttt ttgacagtcc
     1981 ctcctagata ctttcacagt ctaggggggt ttttaaggtt gtcacttgca accttgataa
     2041 gctctctttt taaccattgc tattcgccta gaaacagcac tggaactgtt ttagcggttc
     2101 aactcctctt gtaggatctt cttccctttt ttgaaggtct tatctgaaat ttggcataat
     2161 aaagtgcgct cccatgttct ggaagcgcac tttattatcg cttttactta accttgtatc
     2221 cactcgatga tggaagtgcc gccccaattc ttagattgct tggctaactt ttaagctctt
     2281 gtacaaggaa gaagtctttt tgactatctg ttgattagag gcgagcatta agctctgctg
     2341 ataatgcttc aaaacctggt tttccaagaa gcgcaaacat gttacgtttg taagcttcaa
     2401 cacctggttg atcaaatggg ttgacagcat tcatgtagcc tgaaacagcg atggcaagct
     2461 caaagaagta aatagtgtaa ccaagggtaa actcgtcttg agctggaaga gtgacaaaca
     2521 tgtttggcac gccgccatct gtgtgggcaa gaagcacacc atctgtagct ttcttgttaa
     2581 caaaatcaac gtctttacct tgaaggtaac caagaccatc taggtcttct gctaattcag
     2641 gaatgataac atttttacga ggattatcaa cacgaataac tgtttcaaag agattacggt
     2701 aaccttcttg gataaattga ccaagtgagt gaaggtctgt tgagaagtta gctgaagttg
     2761 ggtaaatccc tttttggtct tttccttctg attcaccagc taattgtttc caccattcgc
     2821 caaagtactg aagagatggc tcgtagttag ccaagatttc agtgatgtag cctttacggt
     2881 aaagcacatt acgaacagca gcgtattggt aagcaatgtt ttcagagatt ttgtctgaag
     2941 aaaggtcttt acgagcagca tttgcccctt ccataagagc agtaatatct gcacctgatg
     3001 cagcaattgg aagcaaacca acagcagtca agactgagaa acgaccacca acattatctg
     3061 gcaccacaaa tgtttcccag ttgttagcgt cagcttctac cttaacagca cccttaactt
     3121 tgtctgtggt agcgtagata cgtttgttag cttcttcttg gccatatttt ttaaccaaaa
     3181 gttctttgaa cacgcgaaag gcaatagctg gttcagtggt tgtacctgat ttagagatca
     3241 cgttgactga gaactctttg tcttgaacgt actcaactaa atcggcaagg taagtagatg
     3301 aaatagagtt tccagcgtaa agaatttgtg gggctttgcg ctctttggca gtttgcaagt
     3361 tagcaaaatg atgatttaaa aaatcaatcg ctgctttggc accaaggtat gacccaccga
     3421 taccgatcac cacaagaact tcgctgtctg ccttaatctt ttcagcagca gtcaagatac
     3481 gtgcaaattc ctctttgtca tagttttctg gaagatcaag ccagcccaag aaatctgaac
     3541 ctggtccagt tccttcacgc aataacttgt cagcttctgt tacttgacct tgtaaaaaat
     3601 caatttcatg ttgtcccgca aatgactcga ggacttttga ataatcaaat gtaatatgtg
     3661 acatgagttt cgctccctta aaattttaag tttcattagt atgataacct tttctatcta
     3721 atatttcaat ggtttttagt ggttttcacc tgtgaaaaag ctaggttttt tgaaaaggtt
     3781 ttcgtttttt cagacataaa aaatgtgtca atgcaagcat attctcatca tagtggtttt
     3841 ttaagagaaa ataggatttc atatttccta aaaatatcat gaaaaaaggc tctgaggccg
     3901 ctttacctta ataacttatt ttaaggattt tatcatttca atacgggtaa taaccccatc
     3961 atgcatatga tcaatgcata aggccacacc cgcaaaggca attgaagccc caatcacctg
     4021 taaatacttt ctcatgctat cttcttaaca ttacttactc tcattatcta aaaatggcaa
     4081 ttctttgaat acttaaaaag caaagggggg agaatagcac tagtacgttt ttaagtagat
     4141 at
//
LOCUS       DAUCWO010000094         3982 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUCWO010000094 DAUCWO010000000
VERSION     DAUCWO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.094"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1441
                     /locus_tag="VGY20_001620"
     rRNA            <1..1441
                     /locus_tag="VGY20_001620"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1493..1565
                     /locus_tag="VGY20_001621"
     tRNA            1493..1565
                     /locus_tag="VGY20_001621"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Ala,seq:tgc)
     gene            1864..>3982
                     /locus_tag="VGY20_001622"
     rRNA            1864..>3982
                     /locus_tag="VGY20_001622"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1143 a          815 c         1140 g          884 t
ORIGIN      
        1 gggtgagtaa cgcgtaggta acctacctca tagcggggga taactattgg aaacgatagc
       61 taataccgca taagagagac taacgcatgt tagtaattta aaaggggcaa ttgctccact
      121 atgagatgga cctgcgttgt attagctagt tggtgaggta aaggctcacc aaggcgacga
      181 tacatagccg acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc
      241 tacgggaggc agcagtaggg aatcttcggc aatgggggca accctgaccg agcaacgccg
      301 cgtgagtgaa gaaggttttc ggatcgtaaa gctctgttgt tagagaagaa tgatggtggg
      361 agtggaaaat ccaccaagtg acggtaacta accagaaagg gacggctaac tacgtgccag
      421 cagccgcggt aatacgtagg tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg
      481 caggcggttt tttaagtctg aagttaaagg cattggctca accaatgtac gctttggaaa
      541 ctggagaact tgagtgcaga aggggagagt ggaattccat gtgtagcggt gaaatgcgta
      601 gatatatgga ggaacaccgg tggcgaaagc ggctctctgg tctgtaactg acgctgaggc
      661 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga
      721 gtgctaggtg ttaggccctt tccggggctt agtgccggag ctaacgcatt aagcactccg
      781 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg
      841 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcccg
      901 atgcccgctc tagagataga gttttacttc ggtacatcgg tgacaggtgg tgcatggttg
      961 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctattgt
     1021 tagttgccat cattaagttg ggcactctag cgagactgcc ggtaataaac cggaggaagg
     1081 tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg
     1141 ttggtacaac gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc aatctcagtt
     1201 cggattgtag gctgcaactc gcctacatga agtcggaatc gctagtaatc gcggatcagc
     1261 acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt
     1321 gtaacacccg aagtcggtga ggtaacctat taggagccag ccgcctaagg tgggatagat
     1381 gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga tcacctcctt
     1441 tctaaggaaa tggaacacgt ttatcgtctt atttagtttt gagaggtctt gtggggcctt
     1501 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagg
     1561 ctccatcagg atacaatcct actaaactta atacaagtga agttgaacac gcaactcact
     1621 tcctaggaaa atagacaatc ttcccttgtg tgcaaggccc acatggtcag attcctaatt
     1681 ttctacagaa gtttcgctaa agcgagcgtt gcttagtatc ctatataata gtccattgaa
     1741 aattgaatat ctatatcaaa ttccacgatc tagaaataga ttgtagaaaa gtaacaagaa
     1801 aataaaccga aaacgctgtg actatttaat aagttttcta gtttaaaaaa actaggttaa
     1861 taaggttaag ttaataaggg cgcacggtgg atgccttggc actagaagcc gaagaaggac
     1921 gtgacaaacg acgaaatgct ttggggagct gtaagtaagc gctgatccag agatgtccga
     1981 atgggggaac ccactaacta atggttagta tccataactg ttaaggttat gagaaggaag
     2041 acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaacgc gattgcctta
     2101 gtagcggcga gcgaaacggc aagagggcaa accgaagtgt ttacacttcg gggttgtagg
     2161 actgcgacgt gggactttaa aaggatagaa gaattacctg ggaaggtaag ccaaagagag
     2221 taaaagcctc gtatttaaaa ttcttttgag ccctagcagt atcctgagta cggcgagaca
     2281 cgtgaaatct cgttggaatc tgggaggacc atctcccaac cctaaatact ctctagtgac
     2341 cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg gagtgaaata
     2401 gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt
     2461 ttgtagaatg aaccggcgag ttacgatatg atgcgaggtt aagttgaaga gacggagccg
     2521 tagggaaacc gagtcttaat agggcgattt agtatcatgt tgtagacccg aaaccatgtg
     2581 acctacccat gagcaggttg aaggtgaggt aaaactcact ggaggaccga accagggcac
     2641 gttgaaaagt gcttggatga cttgtgggta gcggagaaat tccaaacgaa cttggagata
     2701 gctggttctc tccgaaatag ctttagggct agcgtcgatg ttaagtctct tggaggtaga
     2761 gcactgtttg ggtgaggggt ccatctcgga ttaccaatct cagataaact ccgaatgcca
     2821 acgagatatc atcggcagtc agactgcgag tgctaagatc cgtagtcgaa agggaaacag
     2881 cccagaccac cagctaaggt cccaaaataa ctgttaagtg gaaaaggatg tggggttgca
     2941 cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg taatagctca
     3001 ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacagtttac cgaagctgtg
     3061 gatgacacaa aagtgtcatg gtaggagagc gttctatgtg tgaagaaggt gtaccgtgag
     3121 gagcgctgga acgcatagaa gtgagaatgc cggtatgagt agcgaaagac aggtgagaat
     3181 cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg gttagtcggg
     3241 acctaaggag agaccgaaag gtgtatccga tggccaacag gttgatattc ctgtactaga
     3301 gtatatagtg atggagggac gcagtaggct aactaaaccg gacgattgga agagtccggc
     3361 taagcagtga ggtgtaagat gagtcaaatg cttatcttta taacattgag ctgtgatggg
     3421 gagcgaattt tagtagcgaa gttagtgatg tcacactgcc aagaaaagct tctagcgttt
     3481 aatgatactc tacccgtacc gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg
     3541 atcgagagaa ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg
     3601 tgctgactta ggtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
     3661 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
     3721 gaggagggtt tagcgcaagc gaagatctga attgaagccc cagtaaacgg cggccgtaac
     3781 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
     3841 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
     3901 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
     3961 gtatctgtac cacatgtaca gg
//
LOCUS       DAUCWO010000095         3942 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUCWO010000095 DAUCWO010000000
VERSION     DAUCWO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.095"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            24..575
                     /locus_tag="VGY20_001623"
     CDS             24..575
                     /locus_tag="VGY20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ4274443.1"
                     /translation="MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRH
                     YYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYLKDLLRRQESQ
                     KRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSPERMQFQALIRGE
                     RFKGRRALLRKISPYFKEFAQQL"
     gene            762..1511
                     /gene="truA"
                     /locus_tag="VGY20_001624"
     CDS             762..1511
                     /gene="truA"
                     /locus_tag="VGY20_001624"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ4274444.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1501..2265
                     /locus_tag="VGY20_001625"
     CDS             1501..2265
                     /locus_tag="VGY20_001625"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ4274445.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHVGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            2255..2719
                     /locus_tag="VGY20_001626"
                     /pseudo
     CDS             2255..2719
                     /locus_tag="VGY20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
     gene            2738..3298
                     /locus_tag="VGY20_001627"
     CDS             2738..3298
                     /locus_tag="VGY20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ4274446.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQLMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(3363..>3942)
                     /locus_tag="VGY20_001628"
     CDS             complement(3363..>3942)
                     /locus_tag="VGY20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ4274447.1"
                     /translation="FGIPVSSLLAGAGIAGVAIGLGAQGFLSDVVNGFFILFENQFEV
                     GDNVTISDIEGSVFGVGIRTTQIRGFDGTLHFIPNRSITVVSNKSRGNMRALIEIPLY
                     STVNLSQVTRIIDEVNQKELPNHPQIVGKPNILGPQNNSNGQFTFRIAIFTENGEQFK
                     IYHTFYRLYQEALLKEGIQLPTAISYSITSKS"
BASE COUNT         1238 a          581 c          869 g         1254 t
ORIGIN      
        1 atgaaaaaaa caacaggaca ctaatgtcct gttgtttttt tgtggttctc tttatactag
       61 ggatagttaa aaagaaaagg agactcaaaa tgtcgataga gacaagagca gcgtttgaaa
      121 aagttaagcc cattattttg aaattaaagc gacactatta tattcaattg tgggatagag
      181 atgactggtt acaagaagga catattattt tattacagtt actagaaagg tacccagagt
      241 taattgagga agaagagcgt ttatatcgct attttaaaac aaaattttca tcttatttga
      301 aagatttatt acgccgtcaa gaaagtcaaa agcgtcagtt ccataagtta gcatatgaag
      361 agatagggga ggttgcacat gccattccat cgagagggtt atggctagac gactatgtgg
      421 cttatcaaga ggtaatagct agcttagaga accaattaaa ttcgccagag cgtatgcagt
      481 ttcaagcact tatcaggggc gaacgtttca agggaagacg tgctctactt aggaagatca
      541 gtccctattt taaggaattt gcacagcagt tgtaagcaag acctataagg aattgggggt
      601 aaatctatga aaaaaggaga gagagtttgg aggaaatagc tttcttatag cttttaaatc
      661 gttagtagct tagagtaagc ttcaatacgt ctttcatttg tttgggtaca gtttgattaa
      721 aaatagtttc tcttacgttt aagaaatgtt aaaatagtaa catggtaaga tacaaagcaa
      781 cgatttccta tgatgggact cttttttcgg gttttcaaag acaaagacat ttacgtacag
      841 ttcaagaaga gattgaaaaa acactttata aattaaataa cggtacaaaa attataattc
      901 atggtgcagg acgtacggat gcaggagtac atgcttatgg acaggtgatc cattttgatt
      961 tgcctcaaga acaagaagtt gaaaaattac gatttgctct tgatacacag actcctgaag
     1021 atattgatgt tgttaatata gaaaaagtgg ctgatgattt tcactgtcgt tatcagaagc
     1081 atttaaaaac ttatgaattt ttagttgata atggtcgacc taaaaatccg atgatgcgac
     1141 actataccac tcattaccct tatactctta atattaaact tatgcaagaa gctattaacg
     1201 gtctggtagg tacacacgat tttactggtt ttacagcagc aggaacttcg gtacaaaata
     1261 aggtgcgaac gattaccaaa gcaacggtat ctcgggatga aaaaactgat tttttggtgt
     1321 ttactttttc aggaaatggt tttctctata agcaggtgag gaatatggtg ggaactttgc
     1381 tgaaaattgg taatggtcaa atgccagttg aacaagttaa agtgattttg agctccaaaa
     1441 atcgtcagct agctggacca acgatatctg gaaatgggct ttacttgaag gagatttgtt
     1501 atgaaaactg attatatcgt gactatatct gggaacgata ttttaagtgg aggtggcctc
     1561 tatgctgatt tagcgactta cataaggtat gacctacagg cctttgtagc ggtaacttgt
     1621 ttaactacta gatctgaaga gggctttagt ctatttccgg tagctaaaga aattttccgt
     1681 gatcagttga acagttttac gaacgctcca atttctgcta ttaaaattgg tttgttacct
     1741 aatgcggaaa tgtgtgaaat agttttggat tttataaagg ggcatgtggg aatcccagtg
     1801 gttttagatc ctgttctagc atgtaaagaa atagatgatg ttaaaatagt tcctctaaga
     1861 caggagattt tacaattatt gccttatgta actgttgtta ctccaaattt ggtagaggct
     1921 cagctattat ctcaaaaaga gattgtttct ttaaaagata tgcaagaagc tgctaaatat
     1981 ttttatcaac ttggggcaaa acaggtggtt atcaagggtg gtaatcgatt tagtcagaaa
     2041 aaggctattg atctttttta tgacggtaaa gagattgtta ccttggaatg tcctgtactt
     2101 gagaaaaata atattggcgc aggctgtact tttgcatcta gtatcgctag tcagttagta
     2161 aagaaaaaaa cgccactaga agctgtgaaa aactcaaaag agttagtcta ccaagctatt
     2221 ttacaatctg atagatacgg agtaaaacaa agttatgcaa aatagtaaga tacgacaaat
     2281 gagtttaaca gggatattga cagcattggt tgttgtttta ggaagatttg tcatgctacc
     2341 tactccaaca ggttttttaa cgctgttgga tgcaggaatt tatgctgtca gcttttcctt
     2401 tggatcagct caaggagcta ttgtgggagg gttatcaggt tttttaatcg atttagtggc
     2461 aggataccct cagtggatgt ttcatagttt gattgctcac agtgtccaag ggtattttgc
     2521 gggatggaga ggacgcaagc gttggtttgg tgttgtgata ggttctttta tcatgatttt
     2581 ttggtatttt ttaggctctc ttatgcttgg ttatggcctt tcgggatctt tagccggtat
     2641 ttggggaaac gtcatgcaaa atacattagg actttttgtt ggttttatta tctttaaggc
     2701 catattaagg aaaaaaagag ataaggagag tgagaagttg ttgaataatt tagagaaaca
     2761 aacaagggaa atcgttattg atgttgtaga acgctctgct attcaaccag gtaatctttt
     2821 tgttctaggt ctttcatcta gcgaaatttt gggtagtaga attggaaaac aatctagtct
     2881 agaagtaggt caaatagttg ttgaagttgt gttagatgag ttaaataagc gtggtgttca
     2941 tttggctgta caaggttgtg aacacgtaaa tcgtgccttg gtagtagaga ggcatgtggc
     3001 agaatctaag cagttagaga ttgtcaatgt ggttccgaat cttcatgctg gtggcagtgc
     3061 tcagatggct gcttttcaac ttatgtcaga ccctgtcgaa gtagaagaag ttattgcaca
     3121 cgctggactt gatataggtg atactgctat aggaatgcat attaagcgtg ttcagatccc
     3181 tctgattcct tgtcaacgag agctaggagg tgctcatgtg acagctttgg ctagtcgacc
     3241 aaaattaatt ggtggagcta gggctgatta caatatggat attatccgta aatcctaaac
     3301 ccttttttct ggaataaaac aattggtgtt ttttctagaa aaaaggattt tttaagatta
     3361 tattaagatt ttgaggtgat agaatagctg attgcagtcg gtaattgaat gccttctttg
     3421 agtaaagctt cttgataaag tctataaaaa gtatggtaaa tcttaaattg ttctccattt
     3481 tctgtgaaaa tagcaattct aaaagtgaac tgtccattag agttattttg aggccctaaa
     3541 atatttggtt tgccaacgat ttgaggatgg tttggcaatt ccttttgatt cacttcatca
     3601 atgattcgag tgacttggct caaattaaca gtggaataaa gaggaatttc aattaaggca
     3661 cgcatattac cacgagattt gttgctgaca acggtgatgc tacggtttgg gatgaaatgt
     3721 agtgttccat cgaatccacg aatttgagta gttctaattc caacaccaaa gacactgcct
     3781 tctatgtcag agatagtaac gttatctcct acttcaaatt gattctcaaa taagataaaa
     3841 aagccattga caacgtcaga taaaaatcct tgtgcaccta acccgattgc tactccagca
     3901 attccagctc ctgctagaag actagagaca ggtatgccaa at
//
LOCUS       DAUCWO010000096         3865 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUCWO010000096 DAUCWO010000000
VERSION     DAUCWO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.096"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            42..1301
                     /gene="hasA"
                     /locus_tag="VGY20_001629"
     CDS             42..1301
                     /gene="hasA"
                     /locus_tag="VGY20_001629"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HEQ4274448.1"
                     /translation="MPIFKKTLIVLSFIFLISILIYLNMYLFGASTVGIYGVILITYL
                     VIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESLLETLKSVLAQTYPLSEIYIVDD
                     GSPNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSD
                     TYIYPNALEELLKSFNDKTVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSL
                     TGNILVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQS
                     TARCDTDVPFQLKSYFKQQNRWNKSFFRESIISVKKILSNPIVALWTIFEVVMFMMLI
                     VAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMVKHPASFLLSPLYGILHLFV
                     LQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            1337..2545
                     /gene="hasB"
                     /locus_tag="VGY20_001630"
     CDS             1337..2545
                     /gene="hasB"
                     /locus_tag="VGY20_001630"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEQ4274449.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISYDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTNRYDNELQDVKNK
                     VYSRDIFNRD"
     gene            2727..3641
                     /gene="hasC"
                     /locus_tag="VGY20_001631"
     CDS             2727..3641
                     /gene="hasC"
                     /locus_tag="VGY20_001631"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEQ4274450.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PQVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT         1331 a          642 c          671 g         1221 t
ORIGIN      
        1 ccctgtcttt caaggaaatt aaaaaagaaa gaggtgtaat tgtgcctatt tttaaaaaaa
       61 ctttaattgt tttatccttt atttttttga tatctatctt gatttatcta aatatgtatc
      121 tatttggagc atcaactgta ggaatttatg gagtaatatt aataacctat ctagttatta
      181 aacttggatt atctttcctt tatgagccat ttaaaggaaa gccacatgac tataaagttg
      241 ctgctgtaat tccttcttat aatgaagatg ccgagtcatt attagaaact cttaaaagtg
      301 tgttagcaca gacctatccg ttatcagaaa tttatattgt tgatgatggg agtccaaaca
      361 cagatgcaat acaattaatt gaagagtatg taaatagaga agtggatatt tgtcgaaacg
      421 ttattgttca ccgttccctt gtcaataaag gaaaacgcca tgctcaagcg tgggcatttg
      481 aaagatctga cgctgacgtt tttttaaccg tagattcaga tacttatatc tatccaaatg
      541 ccttagaaga actcctaaaa agcttcaatg ataagacagt ttatgctgca acaggacatt
      601 tgaatgctag aaacagacaa actaatctat taacgcgact tacagatatc cgttacgata
      661 atgcctttgg ggtggagcgt gctgctcaat cattaacagg taatatttta gtttgctcag
      721 gaccattgag tatttatcga cgtgaagtga ttattcccaa cttagagcgc tataaaaatc
      781 aaacattcct aggtttacct gttagcattg gggatgatcg atgtttaaca aattatgcta
      841 ttgatttagg acgcactgtc taccaatcaa cagctagatg tgatactgat gtacctttcc
      901 aattaaaaag ttattttaag caacaaaatc gatggaataa atcttttttt agagaatcta
      961 ttatttctgt taaaaaaatt ctttctaatc ccatcgttgc cttatggact attttcgaag
     1021 tcgttatgtt tatgatgttg attgtcgcaa ttgggaatct tttgtttaat caagctattc
     1081 aattagacct tattaaactt tttgcctttt tatccatcat ctttatcgtt gctttatgtc
     1141 gtaatgttca ttatatggtc aaacatcctg ctagtttttt gttatctcct ctgtatggaa
     1201 tattacactt gtttgtctta cagcctctaa aactttattc tttatgcacc attaaaaata
     1261 cggaatgggg aacacgtaaa aaggtcacta tttttaaata atatatgcat cgagtagtta
     1321 gagaaggagt aattttatga aaatagcagt tgctggatca ggatatgttg gattatcact
     1381 aggagttctt ttatcacttc aaaacgaagt cactattgtt gatattcttc cctctaaagt
     1441 tgataagatt aataatggct tatcaccaat tcaagatgaa tatattgaat attacttaaa
     1501 aagtaagcaa ttatctatta aagcaacttt agatagcaaa gcagcttata aagaagcgga
     1561 actggtcatt attgccacac ctacaaatta caacagtaga attaattatt ttgatacaca
     1621 gcatgttgaa acagttatca aagaggtact aagcgttaat agccatgcaa ctcttatcat
     1681 caaatcaaca attccaatag gtttcattac tgaaatgaga cagaaattcc aaactgatcg
     1741 tattatcttc agccctgaat ttttaagaga atctaaagct ttatatgaca acttatatcc
     1801 aagccgaatt attgtttctt gtgaagaaaa cgattctcca aaagtaaagg cagacgcaga
     1861 aaaatttgca cttttattaa agtctgcagc taaaaaaaat aatgtaccag tacttattat
     1921 gggagcttca gaagctgaag cagtaaaact atttgccaat acttatttag cgttaagggt
     1981 agcttatttt aatgagttag acacttacgc agaatcgaga aaattaaata gtcacatgat
     2041 tattcaagga atttcttatg atgatcgaat aggaatgcat tataataacc catcatttgg
     2101 ttatggaggt tattgtctac ctaaagatac gaagcaatta ttggcaaatt acaataatat
     2161 tccccaaacg ctaattgaag ctatcgtttc atcaaataat gtgcgcaagt cctatattgc
     2221 taagcaaatt atcaacgtct tagaagagcg ggagtcccca gtaaaagtag tcggggttta
     2281 ccgtttaatt atgaaaagta actcagataa ttttagagaa agtgctatca aagatgttat
     2341 tgacattctt aaaagtaaag acattaagat aattatttat gagccaatgt taaacaaact
     2401 tgaatctgaa gatcaatctg tacttgtaaa tgatttagag aatttcaaga aacaagcaaa
     2461 tattatcgta actaatcgct atgataatga attacaagat gttaaaaata aagtttacag
     2521 tagagatatt tttaatagag actagccatg tcttcatcta taacaataat attcattttt
     2581 actttaaaag aactgcagtt ggtggtatga ttgagcacta tgaaatagtc gaaaaatcat
     2641 atgaaaacgt aacatgatgt caaatgaatt ccgttctgaa aacgaatgtc actaagccat
     2701 agattataaa aagtgaggag ttactgatga ccaaagtcag aaaagccatt attcctgctg
     2761 caggtctagg aacacgtttt ttacctgcta ccaaagctct tgccaaagag atgttgccca
     2821 tcgttgataa accaaccatc cagtttatcg tcgaagaagc gctaaaatct ggcatcgagg
     2881 aaatccttgt ggtgaccgga aaagctaaac gctctatcga ggaccatttt gattcaaact
     2941 ttgaattaga atacaacctc caagctaagg ggaaaaatga actgttgaaa ttagtggatg
     3001 aaaccactgc cattaacctt cattttatcc gtcaaagcca cccaagagga ctgggagatg
     3061 ctgtcttaca agccaaagcc tttgtgggca atgaaccctt tgtggtcatg cttggagatg
     3121 acttaatgga cattacaaat gcatccgcta aacctctcac caaacaactc atggaggact
     3181 atgacaagac gcatgcatcc actatcgctg tgatgaaagt tcctcatgaa gatgtgtcta
     3241 gctatggggt tatcgctcct caaggcaagg ctgtcaaggg cctttacagt gtagacacct
     3301 ttgttgaaaa accacaacca gaagatgcgc ctagtgattt ggctattatt ggtcgttacc
     3361 tcctaacccc tgaaattttt gatattttgg aaagacagac ccctggagca ggtaacgaag
     3421 tgcaactcac agatgctatc gataccctca ataaaactca gcgtgtcttt gcacgagaat
     3481 ttaaaggcaa tcgttacgat gttggggata aatttggatt catgaaaaca tctatcgact
     3541 atgccttaga acacccacag gtcaaagagg acttgaaaaa ttacattatc aaactaggaa
     3601 aagctttgga aaaaagtaaa gtaccaacac attcaaagta atgtgttggt actttacttt
     3661 tttccttgtt tatagcaact taagtttata tcttgaaaat tgtcaacgtt ccaaaatata
     3721 aaagttttgt gttatacaac tttactagag ggacatatta ataataggat ttatacgtag
     3781 aaaaatgata aacataagta cagcttttgt taaaactaat taacaagaac ttcaaaacta
     3841 aatagttatt tggacgagta tgata
//
LOCUS       DAUCWO010000097         3465 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUCWO010000097 DAUCWO010000000
VERSION     DAUCWO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.097"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1455)
                     /locus_tag="VGY20_001632"
     CDS             complement(<1..1455)
                     /locus_tag="VGY20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ4274451.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVTAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLNSNKSSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNITDQTVIYL"
     gene            complement(1452..3197)
                     /locus_tag="VGY20_001633"
     CDS             complement(1452..3197)
                     /locus_tag="VGY20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ4274452.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            complement(3194..>3465)
                     /locus_tag="VGY20_001634"
     CDS             complement(3194..>3465)
                     /locus_tag="VGY20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4274453.1"
                     /translation="DTKEVSKLLLQQDAKQEIRIHELSCFIQELQQTIVDKTKQQETR
                     EVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRKK"
BASE COUNT         1174 a          593 c          499 g         1199 t
ORIGIN      
        1 aagataaata accgtttggt cagtaatatt attttttctg tctttattaa tatcttctgc
       61 taatttttgg tacttctgag cgatactcgc tattgtttct tgactataat ttgtcggttt
      121 ttccattatt ttcttggtca cctgtcttgt ccaaacatat gctaaagatt tgtacctagc
      181 atttgttgaa aaacctaacc agtttatatc attatcatta tttaaagccg aaataatcgg
      241 aagaccagat aatattttac cttctttttc ataagaaaat ggtattaaca tactacaaga
      301 tacaataaat aaacctaata cagcacttgc ccgtttgaca gggctcataa ataatttccc
      361 tgctaaaact cggccatgta gataccaagc caccacaata caaatagcaa gtcctacaac
      421 catttctaca atcaccgatt ttttaacaaa gcttgtcact aaacctaatt cttgtaacca
      481 aacaaaatca gttactaata acggctcgct tctcattgat tcttttaaat cattaacaac
      541 agaaataatt gttcccagaa taagcaaaaa gaaggtagtt ggccaatatc tattcgttat
      601 cacgtatgct aaaagagcaa ctagtgtaat agctactatc tgaaaaagag tagctccagg
      661 gaaaacataa tatcctagca atgcttcatc accttttacg ccatactgga acgtatagtt
      721 aaaaactaat gctaaaaaca aactagtagt agctgctact gaaaatgaag atttatttga
      781 gttaaggtgt tttagagcat ttaaaaccac gtatatgatt gttgcaaata tgacaagtaa
      841 aactatcaac agtccactta tttttaacag tgcaatttgg ctattacctg ctgttactaa
      901 ttcagaaaat cgagaactta ttagttttaa cagatgtatg tcattaattg gcacaactgc
      961 taaaatcaga gctacagaaa aagtattacc ttgatagcct aatagcccaa atctatcttt
     1021 ttctattgca aaatatgcta ccaatatttt tattaaagtg gccatcacta aaataatgag
     1081 taaagaaggt agagcttgaa attgataaaa ttgattttta gttaatgacc agatatcaaa
     1141 ccgtttatca ttcaggtgct gtgttatttg tacccaatag ctgacaatta gataagatat
     1201 cacaataaaa ctagttttca aaaacaactg tcgactaact tggcgactca aataataaga
     1261 taatccaatc agtgatataa tatatcccac aaaagaaaca tttagtaaat gagtataata
     1321 gtttaccggt tgtgccgtgg agcttactag ttctttcgcc aaactgtaaa aactgaaata
     1381 aaaaccgaaa acaagaagga taatatggtg actaatattt aaccaattgg tttttaaaaa
     1441 tgtgtcttta atcatttaag tttctccatt aaacgcaaga atatatactt cattgcttta
     1501 gcgggaccaa caataatata ttttaatgct cctttgatac cgcccatttg attaaagtta
     1561 acgtaggtat gagctccttc atcttcacgt aaatttaaca gtttattgtc aataaaaggt
     1621 gttaactcat agggattttt aacaatccta aaatcataag aattccccca cgctatatag
     1681 acaagtaacc gctcaatcgc atgtaatata gagttttgtg gtaaaggttc acttggaata
     1741 tcattttgag ttaattctaa atcaaaaagt gattttagag catcgtattt aaaccataca
     1801 aatgttccat aactcataac aaatgtatcc atagcttgaa aatcaatttg ttttttaaca
     1861 tccatttttc tccacaaaga catcatttct tgagcgatta aatgctcatt ccaagcattg
     1921 acaatcttat taaatctaaa aaaacttgga atatctgcga ttataatacc aatatcatct
     1981 gtctcaaaag cgctaagaat actatctgct ggtttcacga gcatatcaat caattctttt
     2041 cgccaagatt ctcctgccca aaaatcagct tcttttgatt ttttcgtatg gaaatgacca
     2101 atataatcat attgagaaag tttatctttt agtaataaca ttggataaat atctcttcct
     2161 ctatttcccg taacgcgtat atcagcagtt ttaccttttc tttgaagtat ttctttgatc
     2221 tcttttcttt tgatatcact atctgttgta ataaaaagat cataatggaa gttccagtct
     2281 tcgaaagctg ttaaaaattc atctagcaaa tcaacgtaaa aaacatgtaa atgaacagct
     2341 accttttgac aagtttgttt tgctaaccgt tggcagttta ggtatttttg agataaaagg
     2401 tacttagcat ctggcaaaga gatttgcgac atatgtgaaa caataagatc tataggataa
     2461 ttggttgttt ctcgtatcag attcagtaag taaggagcga tatgctggtt agcatcaata
     2521 gcttttactt ttataaaagg cacatggtgt tttaaaattg ctgttggatt ataatatgaa
     2581 aaatccggat gtggcatgaa tgacgactca gcttttctag tatcaaagac cgtctgataa
     2641 ctaaagcctt catttaagag aatggatgtt aattgcgttt cataatgttg aatcacttct
     2701 tgaacgtttt catattctat tatcgattgc caaaattgtt gaaaaacttt gttttgaatg
     2761 acttgatttt taaacgtcat aaaataactt tggacatgtt ctttaaaagc ttttgtacct
     2821 ctattattag taattcccca aaagtcaact gtctcttttt cttcaaaatt ttcaaaataa
     2881 ggagccatct cccaaatagg accaaaacaa gtatcgttca taatcgtcaa tgagtcaaac
     2941 tcttccaatt tgtcaaatcc catagtaatc aagccatcgt gccatgcaga aaaatcaaaa
     3001 cctttatttt tgcgttgcaa aaactcatca ataagacagt gatttttgag acgttttaaa
     3061 tcttcgtgac tcactttact atttgagata aaaacaatct tagaaaacag tgatctcatt
     3121 tgttccaatt gatagtaaac atgtgcactt attttattgt atttgttgaa gtgaacatat
     3181 aataaaatac gattcatttt tttctcctaa taaaagcaat gagcttagac gtgtatttcc
     3241 attgacgtga attaataatg gcatggtatt gttgtcttaa ttcagatagt tgattttttt
     3301 gttcttctat gacattttgc aactccactt cacgtgtttc ttgctgcttt gttttatcta
     3361 cgatagtttg ttgtaattct tgaataaagc aactcaactc atgaattcgt atttcttgct
     3421 tagcatcttg ttgtaacaat agtttagaca cttcttttgt atcta
//
LOCUS       DAUCWO010000098         3441 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUCWO010000098 DAUCWO010000000
VERSION     DAUCWO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.098"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            37..744
                     /gene="speC"
                     /locus_tag="VGY20_001635"
     CDS             37..744
                     /gene="speC"
                     /locus_tag="VGY20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ4274454.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEID
                     FKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDN
                     RIINMKNFSHFDIYLEK"
     gene            complement(813..1565)
                     /locus_tag="VGY20_001636"
     CDS             complement(813..1565)
                     /locus_tag="VGY20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ4274455.1"
                     /translation="MATLDEVLSFAKGLADAGQGVDLDNVYGTQCVDLPNWITTKYFS
                     IALWGNAIDLLDSAAAQGMEVVYNAPGVNPRAGAIFVMVTYAHGYGHTGLVIVTSDGY
                     VLHNIEQNVDGNADALYIGGPARYVDRPFEDGTGYILGWFYPPYDSTPAAVTEPSAPV
                     VAQSDGTYVANPETGTFTVRVAALNVRSAPRLDAEIVATYGENMEFNYDGWIDSDGYI
                     WVTYISVTGVRRYVSVGNSENGRRVTNFGTFR"
     gene            complement(1683..2138)
                     /locus_tag="VGY20_001637"
     CDS             complement(1683..2138)
                     /locus_tag="VGY20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEQ4274456.1"
                     /translation="MIIDLTSLIHLFGDLVRTVEIHVFTLFVCFDIITGLTKGITNKR
                     ANSTKGLSGIIKHFLVVLLVYTVYPYLILLGAKPLAVAFVLFFIACYGISIVENWGQL
                     GLPMPSFVRSFFEKLKRDTDQFDIATIKIDKTGVKLEAPQVDLKQKEEE"
     gene            complement(2148..2759)
                     /locus_tag="VGY20_001638"
     CDS             complement(2148..2759)
                     /locus_tag="VGY20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ4274457.1"
                     /translation="MRNWKVIGKYPQYDSTGAVASTHIIITAEDGSVISQPIKQDLTS
                     TNDTEIIKATLEEFKKSEYVEIAMSEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGKVEPDIYKGMLELIEPAKKGEYQAYDVFTVVDDKHEEQ
                     AGEGNLVFVHVNDSFTYETQTLEDLESEDKVTVIKYADLVKQD"
     gene            complement(2762..3190)
                     /locus_tag="VGY20_001639"
     CDS             complement(2762..3190)
                     /locus_tag="VGY20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ4274458.1"
                     /translation="MDLTLKNKDLNTLYSVLDKIKVTNMRANRGRAKLLAKVETKLSE
                     YAKDEIDIIDQYVAKNDDDKWITDDKGNPKIEDTSKLAELNDFLDELASEPIVIKGGE
                     YSKRFIDFLEYLVESEDEFTSEEIVLIDNILEQFEESKGE"
     gene            complement(3199..>3441)
                     /locus_tag="VGY20_001640"
     CDS             complement(3199..>3441)
                     /locus_tag="VGY20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274459.1"
                     /translation="KVDQAEIYGDDIVFSDDFNIDRGFKMRPSLMPKMVDLNKMYQAI
                     LALGRCWLHANNTAWSWNFDTRSAIIAEYNAHINNL"
BASE COUNT         1047 a          717 c          601 g         1076 t
ORIGIN      
        1 tctcgtacga gtaatacatt taattaagga gaaaaaatga aaaagattaa catcatcaaa
       61 atagttttca taattacagt catactgatt tctactattt cacctatcat caaaagtgac
      121 tctaagaaag acatttcgaa tgttaaaagt gatttacttt atgcatacac tataactcct
      181 tatgattata aagattgcag ggtaaatttt tcaacgacac acacattaaa cattgatact
      241 caaaaatata gagggaaaga ctattatatt agttccgaaa tgtcttatga ggcctctcaa
      301 aaatttaaac gagatgatca tgtagatgtt tttggattat tttatattct taattctcac
      361 accggtgagt acatctatgg aggaattacg cctgctcaaa ataataaagt aaatcataaa
      421 ttattgggaa atctatttat ttcgggagaa tctcaacaga acttaaataa caaaattatt
      481 ctagaaaaag atatcgtaac tttccaggaa attgacttta aaatcagaaa ataccttatg
      541 gataattata aaatttatga cgctacttct ccttatgtaa gcggcagaat cgaaattggc
      601 acaaaagatg ggaaacatga gcaaatagac ttatttgact caccaaatga agggactaga
      661 tcagatattt ttgcaaaata taaagataat agaattatca atatgaagaa ctttagtcat
      721 ttcgatattt atcttgaaaa ataattcatc atacacaaaa aaccgcccag aaattatctg
      781 agcggttttg tcttatctcg gagctttacc tcctatctaa aagtaccaaa gttggtcaca
      841 cgtcgtccgt tttcggaatt tccgacggac acatatcgtc taacaccagt aacactgatg
      901 tatgtcaccc aaatataacc atctgagtca atccaaccat catagttaaa ttccatgttt
      961 tcgccataag ttgccacaat ttctgcgtct aatcgaggcg cagaacggac gtttaaagca
     1021 gcaacacgaa cagtaaaagt acctgtttca gggttagcta cataagtacc atctgactgt
     1081 gcaaccactg gagcgcttgg ctctgtcact gctgctggcg tactatcgta aggagggtaa
     1141 aaccaaccca aaatatatcc agtgccatct tcaaatgggc ggtcaacata tcgagctgga
     1201 ccaccgatgt aaagggcgtc agcattacca tccacgtttt gctcgatgtt atgcagaaca
     1261 tatccgtctg acgtcacaat aactaatcca gtatgaccgt aaccatgagc ataggtcacc
     1321 atcacaaaga tagccccagc tcgtggatta actccaggag cattatagac cacttccatt
     1381 ccttgggcag ctgcactatc tagtaagtca atagcattgc cccaaagggc aatgctaaaa
     1441 tattttgtcg tgatccagtt tggcaagtcc acgcactgcg taccgtaaac attatcaaga
     1501 tcaactcctt ggccagcatc cgctaatcct tttgcaaaag acaagacttc gtctaaagtt
     1561 gccatatcag ttacttcctt tcttcaaaaa atgggcttaa aatcaaagca atcacagata
     1621 atggcacata cagtattgcg attgctataa ctaatgctaa tcgtgtgact gcttgtctca
     1681 tgctactcct cttctttttg ttttaaatca acttgtggcg cctcgagttt aacacctgtt
     1741 ttatcaattt tgattgtcgc aatatcaaac tggtctgtat cacgtttgag cttttcgaaa
     1801 aatgatctga caaaacttgg cattggcaaa cctagttgtc cccaattttc tacgattgaa
     1861 ataccataac aggcgataaa aaataggaca aaggcgactg ctaaaggctt agctccgagc
     1921 aagattaggt aaggatagac agtatagact aacaacacaa ctaaaaagtg tttaataatg
     1981 ccagatagcc cttttgtact gtttgctctc ttattagtaa taccttttgt cagtcctgta
     2041 ataatgtcaa aacagacaaa aagcgtaaaa acgtggattt cgacggtacg aactaaatcc
     2101 ccgaaaaggt gaataagact tgttaaatca ataatcatct aaccacctca atcctgctta
     2161 actaagtccg catacttaat gactgtgact ttatcctctg attctaagtc ctctaaggtc
     2221 tgtgtctcat aagtaaacga gtcgttgaca tggacaaaga ctaagttgcc ttcgccagct
     2281 tgctcttcgt gtttatcgtc gaccaccgta aagacatcat aggcttgata ctcacctttt
     2341 ttggctggct caataagctc taacatacct ttgtagatat cgggttcaac tttcccacca
     2401 cttgtcaata cgtggatggt ttgcaagtta atcatccgct gtgtacgctc tgcggacacc
     2461 ttagctagtc cagcggctgt ttgggctgtt ttagcagtct tagcggtttc ttgtgagatt
     2521 ttttccaagt catcaacttt ttggactgct tcgctcatag cgatttcgac atattcggat
     2581 tttttaaatt cttccaaagt agctttgata atctctgtgt cattagttga ggttaagtcc
     2641 tgcttgattg gttgagagat gactgagcca tcttctgctg tgataatgat gtgtgtactt
     2701 gcgactgctc ctgtgctgtc atattgtgga tatttcccta ttactttcca attgcgcata
     2761 attattctcc tttactttct tcaaattgtt ccaaaatatt atcaattaag acgatttcct
     2821 ctgatgtaaa ctcatcttca gattcgacta aatattccaa aaaatcgata aagcgcttag
     2881 agtactcgcc acctttgatg acgataggct cgcttgctaa ctcgtctaaa aagtcgttaa
     2941 gctcagctaa ctttgaggtg tcctcaattt ttggattacc tttgtcatct gtaatccact
     3001 tgtcatcatc atttttagcg acatattgat caataatatc gatctcatct ttggcatact
     3061 cgcttagctt agtctctact tttgcaagca gcttagcgcg cccacgattt gctcgcatgt
     3121 tcgtgacttt gattttgtct agtacactat atagtgtgtt taaatctttg tttttaagcg
     3181 ttaaatccat tgtttctcct ataagttatt aatgtgtgcg ttatattctg cgatgattgc
     3241 gctgcgtgta tcaaaattcc acgaccaagc cgtgttatta gcatgcagcc agcagcggcc
     3301 gagagccaaa attgcctggt acatcttgtt taagtcgacc atttttggca ttaggctagg
     3361 ccgcatctta aagccacgat cgatgttaaa atcgtcgcta aagacaatat catcaccata
     3421 aatttcggct tgatcgacct t
//
LOCUS       DAUCWO010000099         3432 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUCWO010000099 DAUCWO010000000
VERSION     DAUCWO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.099"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            137..910
                     /locus_tag="VGY20_001641"
     CDS             137..910
                     /locus_tag="VGY20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274460.1"
                     /translation="MLYLNTQTDSDYKEIIFGDIAKFVENMFYHYFSSILFRDLETVD
                     KRMYSFSDDDLISIQSSCLRLSKTFANFNIQRKNFLASDETEMDTFHKKDDIDITVGE
                     KSYNLARSFRTSTINELTVFNFEEMFDIIWLMLGDNLIKSFEVNVCGILFELNGNGIP
                     STFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFLVDDILERMYKENIPK
                     SYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            976..1728
                     /locus_tag="VGY20_001642"
     CDS             976..1728
                     /locus_tag="VGY20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ4274461.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            1725..2327
                     /locus_tag="VGY20_001643"
     CDS             1725..2327
                     /locus_tag="VGY20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274462.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2803..3084)
                     /locus_tag="VGY20_001644"
     CDS             complement(2803..3084)
                     /locus_tag="VGY20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4274463.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(3091..3135)
                     /locus_tag="VGY20_001645"
                     /pseudo
     CDS             complement(3091..3135)
                     /locus_tag="VGY20_001645"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1139 a          647 c          544 g         1102 t
ORIGIN      
        1 agaaaattat tcacaagaaa tacctactcg tatcaaatgt ttaagaaaaa agtataattt
       61 aaagtaaagt gacctaggta atgcaggtca agttagccaa gttgaaaagg gaaagcgaca
      121 agttaccgct tcaatattgc tttatttgaa tactcaaacc gactcggact ataaagaaat
      181 tatttttgga gatattgcca agtttgttga aaatatgttt taccactact tcagctctat
      241 tttatttaga gatttggaaa ctgtagataa aagaatgtat tctttttcgg acgatgactt
      301 aatttcaata caatccagct gtcttagact ttccaagaca ttcgcaaatt tcaatattca
      361 aagaaaaaat tttttggctt ctgatgaaac tgaaatggat acttttcaca agaaagatga
      421 cattgacatt actgttggtg aaaaaagtta taacctcgct agaagtttta gaaccagtac
      481 catcaatgaa ttgactgtct ttaattttga agaaatgttt gatattattt ggttgatgtt
      541 aggggataat ttaattaaat catttgaagt taatgtctgt ggtatcctat ttgaattaaa
      601 cggaaatggc ataccctcaa catttaggca agaaaacatt gatcccctca tcaataaatg
      661 gtggtatgat aatgtttcga cagaaattat ccctaaccta ataaaaaaac tcaaagaaaa
      721 tccactattt aatatcggat tcctggttga tgatattcta gaacgaatgt ataaagaaaa
      781 tatcccaaaa tcttacctta cgtccgtccc gttagttatt tccaaaaaag caaggtcaac
      841 tttatcgatt aagtcaaacg gaagtcaaaa aatagataaa tcaaaatttg cacagatttt
      901 gtccctttag agacggatga cttacacatt caacgtggat tattcttccc ttggagaaag
      961 gggatttcag aacgcttggc gattcctgat ttagataaat tttcatctag cttacaacag
     1021 gggaaaattc ctgtcataaa gcacttcgaa ctcaatttag ataaggggag tcactgggct
     1081 attgctggaa attcaggttc tgggaaaccg tacgctctca cttacttctt gagtgtcctg
     1141 aaacctatgt ccggcctaat tatcattgac ccaaaattcg atacccctag ccaatgggca
     1201 agagaaaata aaattgccgt tatccaccca gtagaaaacc attcaaaatc tgatttcgtt
     1261 tcacaagtca atgaacaatt aaaccagtgt gcaactctca ttcagaaacg acaagctatc
     1321 ttgtatgata atcccaatca tcaatttacc catctaacta ttgttattga tgaagtcctc
     1381 gctctatcag agggagtcaa taagaatatc aaagaagctt ttttctccct actatcacaa
     1441 attgccttgt tgggacatgc tactaaaatc catctattct tgggaagcca gcgttttgac
     1501 cataacacta ttcccatttc agtgagggaa cagctaaacg tattattgca aattggaaat
     1561 atcaatcaga aaaccactca attcttattt ccagacctag atccagaggg gattgtcatt
     1621 ccaacaggac acggaacagg tatcattcaa gttgtcgata atgaacactc ctatcaagtt
     1681 ctacccttac tctgtccaac ctactatact aagcgaggta tcctatgacc actaaaacct
     1741 acttcatgag aagcttcaat ctcatattat acttactgct tattgctcca atctgctatg
     1801 tgttcggttg gctgttcaat cttgtttcag ttcaattatc taccgtcaca tttgtcaatc
     1861 tggatacggt taaaaacgtt actgatacca ttagttctgt atgttatgga ttcgctctta
     1921 tttctttact tctagcctta ttatctggat taaagatgat acaacgcttg aatacagata
     1981 gccttgggaa ctactgccaa tctatttatc gcacctttct attaagacac ttccttttcc
     2041 agcgtgaacg agtacagaag gtaagcaact ttgaacacca gaccgttacc actatcaatc
     2101 caatccataa cgggtttaac cgagctgtac gtaagtgtat cgtagatata cgaaaagatg
     2161 ccattactgt atttctaaaa gttcctagag accaacaggg gcaaaaaatc ctaaaggata
     2221 cgggagtaca actgaaagaa gagattgtca tccaacacac agactattat ttctcttctc
     2281 ctgttcgagt tcgtaaccaa ttgtggttta ttggtcaaaa gcgttaattc cttggggctg
     2341 tggcttgcgt taagcaaaag ccagacagcc ccttttttat attcttatct atcatgacct
     2401 attggggtta ctacgacaac gaaagttaga agctgtgggt atttatttat cttgaaaaga
     2461 ttgctgaact aacgcaataa ctactctacc attcactcta ccttttatta agcaactatc
     2521 aataaatatt gatacaataa ggtttataac agctaaaata gaaaatactt aacctttaca
     2581 gaagtaaaat aattgagatg agaaaagcct atgaaatgaa cgtttcaaag gcttttttcg
     2641 tgtttaaaaa aacaaatgaa aaaacaaaat agagagtagg gttattaaca ctgtcaatcc
     2701 tgtgtacgca ataacaatcc atgctttttg ataaggatat tttgcttttt ctttaatctt
     2761 atcaatctga tctaagctct caccctttgc tatagcttca agttatcggt aagtctgaac
     2821 atcatatgtc tttttaaatc gctcaattct taaggcgata cgcatgatta tcaaccataa
     2881 gatagcaacc ggaccatatc cccaccaacc taaccaagac attataggat aaatactgat
     2941 agcaattagg agtgtcccaa ataacataat atgcgtattc ctatcacatt gtttaatctc
     3001 ctcatcctta ataattaaag tcatttttgg gatatcttct gctattagtt gctctaaagg
     3061 aacctgtaag gacttactta acatcaaaag attttgcagg tctggatagc ttttaccgtt
     3121 ttcccagttt gagatagtct tcttgaaaca tacatcattt cagcaagctc ttcttgtgac
     3181 atctgtaatt tttttcaata ataaggtaac tggtttttaa agcccataat aaatctcctt
     3241 tttaatagct actataatcc ttcttcaata agtttactat caaaagtctt tgacctaagc
     3301 tttttttcgt ttatcttttt gataattatc gtaaggttta cagtctttgt aaaatttatt
     3361 tacaactttg acaatcaaac ttgacttgat cacaaagaca tggtgtttaa ggaaaatatc
     3421 ttcaaaattg aa
//
LOCUS       DAUCWO010000100         3267 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUCWO010000100 DAUCWO010000000
VERSION     DAUCWO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.100"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(106..453)
                     /locus_tag="VGY20_001646"
     CDS             complement(106..453)
                     /locus_tag="VGY20_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEQ4274464.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            complement(443..1570)
                     /locus_tag="VGY20_001647"
     CDS             complement(443..1570)
                     /locus_tag="VGY20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4274465.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKGI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(1567..2547)
                     /locus_tag="VGY20_001648"
     CDS             complement(1567..2547)
                     /locus_tag="VGY20_001648"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ4274466.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAADVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(2687..3265)
                     /locus_tag="VGY20_001649"
     CDS             complement(2687..3265)
                     /locus_tag="VGY20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ4274467.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLFQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
BASE COUNT         1027 a          564 c          612 g         1064 t
ORIGIN      
        1 aagtaagtta tgacagtcaa aatgaacaac atgaattaat agcaatagaa gcgaaatcat
       61 ttattgacat tgtgatatga ctaaaaaaac aaaatttaaa gttgattaga cgactgtttt
      121 gctaaaaatt tcacgttcta caaggctgtc catcaagaaa taaaatttat cttgaaggat
      181 ttgattatct tttgttttaa aaatattaac taatctaaga ccagattgat cggtgatagc
      241 taatttaaaa cctgtgagtt ctttattaat agtgattttt aaagggaaac catcccctga
      301 attaggtatt gtttcgagta aacgtattaa ttggtagtgc cctactttag tttgactaaa
      361 tgtagtatct cttagggtat attttttaat gtttggactg attgtataaa ggtatttaca
      421 gtctttaagc ttaatttctt tttcaaaagc catctgttcc tcctaaaata ttttttaatg
      481 tttgagctaa ttgattaaca tcttcaatgc tattttgatc tgaaaaactg atacgaattg
      541 attcttttaa acgagaagag tcatccccat agtacgcagc taaaacatga cttggattaa
      601 cagctccagc agtacaagca gaaccagttg atactgcaat gcctgctaag tcaagttgtg
      661 ttagtaaaat agtattttgg tagcctaaaa atccaatatt gagaacatga ggaagataat
      721 gagtaccttg attaatatag tagggtagtc cttctaggag gctaatcaag tgatgacgta
      781 aactgataat gtgatcagtt gattggtcaa gacaagtcat agcatcagtc aaagcttgtg
      841 ccataccaat aatacctaac atattttcag tacttgcgcg tcgctttcct tcttgatcac
      901 ccccgtgaag aagagggtcg attggttgtc cgttactgta taaaaagccg catcctttag
      961 gaccatgaaa tttgtgagca gaagcagata aaaagtcaat cccaagttcg ctaggaataa
     1021 tcttgagttt gccaacagct tgaacggcat caacatgaaa agcagcttga tggtctttga
     1081 gcagattccc aatgccctta ataggtaaca ggtcacctgt ttcgttattg gcatacatta
     1141 tgcttaccaa aatggtgtca tctctcaagg cttgttttaa gtcagataaa tttatttgtc
     1201 cattttgaca aggcaaataa gtgacctcaa aaccaaatcg ctcttcgaga taggccatgg
     1261 tatgaagaac agagtggtgc tcaatagtag ttgtgatgag atgtttgccc tttgcttgat
     1321 gagctaaagc atagcctttt atggccatat tattgctttc agttccaccg gacgttacta
     1381 taatttgctg ttcacttgcc cccaagtttc tagcgatagc ttgtcggcat tcacgaagga
     1441 ttttattggc tcggcgcccg tagaaatgaa tactagaggg gttaccaaag ttatcttgca
     1501 tagctgctgt catcgcccta atcacattag gactaagtgg tgtggtggcg gcgttatcaa
     1561 agtaagtcat gcgttatttt tgcctttagg ttgataagaa aaaagtggac ttaagggtct
     1621 tctttcatgg atacgaataa tagcttctgc gattaaatca ctggcgctaa gataagtaac
     1681 gttttctggc acacgatttt tagttttaac agaatctgta acgataattt ctttgattgg
     1741 agctgtttct aatacatcag cagctccacc agcaaataag ccatggctag cgaccgcgta
     1801 agtatcagta gcgcctgagc gttcaagaat tttagcagct tctgcaaagg ttttcccagt
     1861 atttaaaata tcatcaatta aaatggcttt tttaccagaa acatcaccaa taatataacc
     1921 ttgttcacgt tcagaatcat cttgtgcata gtcgataata gcaatcgggg aatcaagata
     1981 ttcagccaag cttctagcac gtttaattcc agaattttta gggctaacga caacaacatc
     2041 agaacctgat aagcctaatt tactgtaacg ttctgcaaaa agagggactg taaagaggtt
     2101 atccactgga atatcaaaaa aaccttgtac ctgaacagca tgtaagtcaa gcgtcactac
     2161 acgatcaata ccagctttag tcagcatatt agctactagt ttagcagtaa tcggttcgcg
     2221 gggcttagcg acacggtctt gacgagagta accaaagtat ggcaatacaa tattgacggt
     2281 attagcactt gcacgtttac aagcatcaat catgatgagt aattcccaaa gattatcatt
     2341 gacaggaaaa ctagtggatt gaataatata gatatcatct ccacgaactg tttcttcaat
     2401 attgatcata atttctccat cggaaaattg acgtgaagac atttttccaa gagggattcc
     2461 agcagcttta gcaatttttt cagcaattgg aagatttgat gtgagtgaga acagtttgat
     2521 ttgcttgtca gcatatcgtt cagtcatgat agatttagct ccttaacttc aataaaattg
     2581 ggtaaactgt tttattaatg agtcacatta cccaaaagtg tttaattgac atccctattt
     2641 taacagaaaa acagaaaaat ttcagttttt ctgtcgcttt tagccattat ttatcgttga
     2701 acttttttaa ggtattaata caccgagcta ctttgctttt agcatatttg aaagtaatgt
     2761 gatgttcaga taaaaaagaa tcaaaatcaa tctttccttg aagagcgtca cttacttcta
     2821 attctaattc atagtctttg gtattggcat ataggttata gtctaaggcc aatttaccta
     2881 tcggtatcac tgtctcgcga cgaacggtag ttaaattgcc aaaagttacc agtgcagatg
     2941 gtttgatacc ttttgaaata ataatatcaa gagcagttga ttcaggaaga ttcccatgtt
     3001 taatcatatc ttttgcttgt tcaagaaaaa gagggacatt gtactcacga ttccccactt
     3061 tttctggcac ttttaaggtt aattcggcgc tatttacaaa tgttcgaata cgtaatgaca
     3121 ttttgttagc ttttaaatca aatgcttttg tatcaatata gtaattagtt tgtgtaacag
     3181 gcgtcacatg tttcatttga aataaaagac ggttatactc attttttgta agtagagttt
     3241 tatattcgat ttctaggtta gtcatgg
//
LOCUS       DAUCWO010000101         3215 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUCWO010000101 DAUCWO010000000
VERSION     DAUCWO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.101"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(97..945)
                     /locus_tag="VGY20_001650"
                     /pseudo
     CDS             complement(97..945)
                     /locus_tag="VGY20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(1233..3125)
                     /gene="sfbI"
                     /locus_tag="VGY20_001651"
     CDS             complement(1233..3125)
                     /gene="sfbI"
                     /locus_tag="VGY20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEQ4274468.1"
                     /translation="MNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLVCAGAIGFG
                     QVAYAADEKTVPNRINSNPEFPWYGYDSYSGRLLRYHNLKVNLNGSKEYQAYCFNLKR
                     FEPKKEESSSPNWYKKLDGSTETFKKYAENPRFSGEELRRHILKVLYNGYPNSNEIMK
                     GIDPLNAILVTQNAIWYYSDSAPINDINNFFTSEANDLNIPPQQLTLMREALRKLISS
                     DENLVKQVPSNFKLSIFESSDKSYQNLLSAEYVPDDPPKPGDTSEHNPKTPELDGTPI
                     PEDPKRPDESSEPALPPLMPELDGEEVPEVPSESLEPALPPLMPELDGEEVPEVPSES
                     LEPALPPLMPELDGEEVPEVPNESLEPALPPLMPELDGQEVPEVPSESLEPALPPLMP
                     ELDGEEIPEVPSESLEPALPPLMPELDGQEVPEKPSVDLPIEVPRYEFNNKDQSPLAG
                     ESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFT
                     KDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIIEDTR
                     PKLVFHFDNNEPKVEENREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKN
                     KQNNKV"
BASE COUNT          896 a          670 c          567 g         1082 t
ORIGIN      
        1 atgagattta attaaacttc atcaaagggg atagtaacta ccagattatt ttacttttac
       61 aacagtttca gtacgataat gaagattcag ggcaagttag tttttaatca cttttttatc
      121 agtcatcttt gaatacataa atactaaaat aatagctata accaccccga aagcagcaca
      181 tactacttct gtccacattc ttgatttatc cacgagtttc attgcttttt tagcctgttg
      241 atactttttg taatgaaatg caattctatg acctctgacc aatagacgct tggtattaac
      301 accatatggt gtgcaggtaa ccagagtagc ataatctttt cccattacct cagaaagaga
      361 tgtgacttga tctggttcaa cagttagtat ctgatcaact ttataagcca atactttatt
      421 taacacacga aagtaaaaag tatctcccct tttcactaaa ttcaaattag taaacatttc
      481 tgccgatgga aggcctctat gagcagaaat aaccgtgtga gtcccgtcgc caccaactgg
      541 taaggcactt ccaaataagt gacctgctcc tttagttagc acctcgtctg tcgtatagtg
      601 gtatatgggt aacgtcactt taatggagga cacttcaaca tattccataa tatcattgtt
      661 ttcaatttga agtaggcttt cgtagttttt atcatgaatg ccatccctaa atgaaaaagc
      721 atctggaacg ggctgaatcc caagcctagc gttatacgcc ttagcctttt gccacatctc
      781 gtccatttct gagggcttct tttgagtcac ttgtttgtga taagttgata acaattaacg
      841 atcacgatag acattccact gactacttag cgtagggtaa agaaaagtgg ctgttcccaa
      901 aaaaaataat aagagtagcc ctagattttt tagtctttct gtcattgcag ttccttatca
      961 ataaaaatcg agactgccta aagacagtct cgattgattt cgacttttga aagcgttaag
     1021 ctttacgacg tttaacaata acttgatttc ttgtaagtct attatacata gaatacacca
     1081 ttttttagcc atcctacttg ctctgaatta tctgtcacct tattattatc agatgtataa
     1141 gtagtacctt ttgaattaac atttgtcttg actgtagcag ttatccacta ttcagcatat
     1201 ttgcgcatta tttcttatgg aacactttta cgtcagactt tattgttttg tttgtttttt
     1261 aaaaggctaa aaatagaaag tactgacaaa ataaggattc caagaaaggc taaaacattt
     1321 ccaatatctc ctgttgcagg aaggatgggt gttatatttt ttgtaggttt ttcccgattc
     1381 tcttccactt tgggctcatt attgtcaaaa tggaacacta acttcggacg cgtatcttca
     1441 ataatagtca ctgtttcact gaaaccactc atgcctgttt gagtgtcttt agtaaattca
     1501 acagactcac tttggcctcc catcaacact cctggctctt tcgtatcttc tgtctcaacc
     1561 tgaggagttg tttgaccact catgcctgtt tgagtgtctt tagtaaattc aacagactca
     1621 ctttggcctc ccatcaacac ttctggctct ttcgtatctt ctgtctcaac catatttcct
     1681 gaaaaacctg tttcattcgg aagtttttta tcaatatcaa cagggttctg ttgatttcca
     1741 tagacttcgg taatatactc cgtctcacca gactcacccg ctagaggtga ctggtcttta
     1801 ttgttaaact cataacgagg aacttcaata ggtaagtcaa cactaggttt ttcagggact
     1861 tcttgaccat ctaactctgg cattaatggg ggaagcgcag gttctaagct ctcgcttgga
     1921 acttctggga tttcttcacc gtctagctct ggcattaatg gaggaagcgc aggttctaag
     1981 ctctcgcttg gaacttctgg gacttcttgg ccatctagct ctggcatcaa tgggggaagc
     2041 gcaggttcta agctctcgtt tggaacttct gggacttctt caccatctag ctctggcatt
     2101 aatgggggaa gcgcaggttc taagctctcg cttggaactt ctgggacttc ttcaccgtct
     2161 agctctggca ttaatggggg aagcgcaggt tctaagctct cgcttggaac ttctgggact
     2221 tcttcaccat ctagctctgg cattaatggg ggaagcgcag gttctgaact ctcatctgga
     2281 cgttttgggt cctcgggaat tggagtgcca tccaactcgg gagttttagg attatgttct
     2341 gacgtatcac caggtttagg aggatcatca ggtacatatt cagcacttaa aagattttgg
     2401 taggacttat cagaagactc aaaaatacta agtttaaagt tcgatggaac ttgtttaacc
     2461 aaattttcat cagaggaaat tagttttctc agagcttcac gcattaacgt tagctgctga
     2521 ggtggtatat taagatcatt ggcttcacta gtaaagaagt tattaatatc attaatcggt
     2581 gcactatccg agtaatacca tatggcattt tgtgttacta agatagcgtt gagagggtct
     2641 atccccttca ttatttcatt actattagga tatccattgt agagaacctt caatatatgt
     2701 cgtcttaatt cctctccact aaacctaggg ttttcagcgt attttttaaa agtttctgta
     2761 ctcccatcta attttttata ccaattagga ctgctacttt cttctttttt tggctcaaac
     2821 cttttcaggt taaaacaata cgcttgatac tccttacttc catttagatt tactttcaga
     2881 ttgtgatatc ttaacaatct tccgctatac gaatcataac cataccaagg aaactcagga
     2941 tttgaattaa tacgattcgg cacagtcttc tcatccgcag cataggctac ttgaccaaaa
     3001 ccgatagcac ccgcgcatac caaaagcata aaaaagactc ccactaaagt gacagcaaat
     3061 cgcctttttc tttttgtgtg tgccaaaaaa ccggcttctt tattcaaaaa cattttgtta
     3121 ttcattttct ctcctctcaa aaacatatat gagcttacca cgttattgtc aagaaagaca
     3181 attttttgat tatatttttc accaaattaa tgttt
//
LOCUS       DAUCWO010000102         3178 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUCWO010000102 DAUCWO010000000
VERSION     DAUCWO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.102"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            225..1241
                     /locus_tag="VGY20_001652"
     CDS             225..1241
                     /locus_tag="VGY20_001652"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ4274469.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            1274..2173
                     /gene="galU"
                     /locus_tag="VGY20_001653"
     CDS             1274..2173
                     /gene="galU"
                     /locus_tag="VGY20_001653"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ4274470.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(2272..2943)
                     /locus_tag="VGY20_001654"
     CDS             complement(2272..2943)
                     /locus_tag="VGY20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ4274471.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         1062 a          656 c          580 g          880 t
ORIGIN      
        1 aaatatcttt atttttaaaa ataagtttcc gaacttaaat aactaaaaag aacaaggcaa
       61 agccatgaca acagctcacc tgatgaagct tattaaacaa cattattaat atagctagct
      121 ctcttgagaa cgccttatta gccaagacca tatacctttt ctaatagagc taatcaaggt
      181 gttatgttat aatagagtga ctagctcttg aaggagaatt tagaatgaca aaacaaaaag
      241 tagctatttt aggacctggt tcttggggaa ctgctctttc tcaggttctt aatgacaatg
      301 gccatgacgt ccgtttatgg ggaaatattc ctgatcaaat tgaagagata aataccaagc
      361 ataccaatag acactacttc aaagacattg tgttagacaa aaacattacg gcaactcttg
      421 atttaggtca agccctatca gatgttgatg cggtgctttt tgttgtgcca actaaagtaa
      481 cacgattagt tgctagacaa gttgccgcca ttttagatca taaagtagtg gtcatgcatg
      541 cctcaaaagg tctagaacca gagacccacg aacgtttatc aactatccta gaagaagaaa
      601 ttccagctca ttttcgtagc gaagtggtcg ttgtatcagg tcctagtcat gctgaagaaa
      661 ctatcgttcg cgatattact ctcatcacgg cagcctcaaa agatattgaa gctgcaaagt
      721 atgttcagtc cctctttagt aaccactact tccgacttta taccaatacc gacgtcatcg
      781 gagttgaaac agctggtgcc ctcaaaaaca ttattgctgt tggagcagga gctctccatg
      841 gacttgggta tggagataat gccaaagcag cggtaattac tcgtgggtta gctgaaatca
      901 cccgtctagg cgtcaagctt ggtgctgacc cgttaaccta cagtgggtta tctggtgttg
      961 gtgatctaat tgtaacaggt acttctgtcc attcacgtaa ctggcgggct ggagctgccc
     1021 ttggacgtgg tgaaaaatta gaagatattg aacgcaacat gggtatggtt atcgaaggca
     1081 tcgcaacaac taaggtagcc tatgaaattg cccaagacct cggagtttac atgccaatta
     1141 caacagctat ttataagtcc atctacgagg gcgctgatat caaagaaagc atcctcggca
     1201 tgatgtcaaa tgaatttcgt tctgaaaatg aatggcatta agccatacgt cactaaaata
     1261 agaggaggtg ctgatgacca aagtcagaaa agctattatt cccgcagcag gtttaggcac
     1321 acgtttttta cctgcaacca aggcccttgc caaggagatg ctacctattg ttgataaacc
     1381 aactattcaa tttatcgttg aagaagctct aaaatcaggt atcgaagaaa ttcttatcgt
     1441 tactggaaaa tccaaacgct ccatagaaga tcattttgat tctaattttg aattagaata
     1501 taatttacag gctaaaggaa aaatcgaact tctaaagtta gtagacgaga ccacttctat
     1561 taatcttcac tttattcgac agagtcatcc aagaggatta ggagacgcag ttcttcaagc
     1621 aaagaccttt gttggcaatg aaccttttgt tgtcatgcta ggtgatgatc tgatggatat
     1681 tacaaatcca aatgtaaaac cattaaccaa acaattgatc gatgactatg aagaaactca
     1741 tgctgcaact attgccgtca tgagggttcc tcacgaggat gtctctaact acggtatcat
     1801 tgctcctcaa gcaaaagcag taaaaggact ctatagtgtg gatacttttg ttgaaaaacc
     1861 acaacctcaa gatgcaccta gtgatttggc aattattgga cgttacttgc tcacaccaga
     1921 aatttttagt attttagaaa aacaagaacc tggtgcaggc aatgaagttc aactgactga
     1981 tgctattgat accctcaata agacacagcg tgtctttgct cgtgagttta aagggaaacg
     2041 ctatgatgtt ggcgataaat ttgggtttat gaaaacttct ttggattatg ccctgaaaca
     2101 ccctcaagtc aaagatgact tgaaagccta catcatccaa ctgggcaaag cactagagaa
     2161 aacaaaacca taaaaataag gagggaaaat ccctccttat ttttatggtt tttgagtcgt
     2221 ttcatcctac tagccatctg agaagtaaca gtgctaacca ctcacttatt tttataaaaa
     2281 cccaagcact aacacaccaa ccaaaataat accatagctc actaaagcca acattcgctt
     2341 ggttttcttc actgttatca ctctcatctt agttggaaaa actatactca gtaacgcacc
     2401 acccacgaca ccgccaatat ggcctgccat actcacgttt ggcataaaaa gattcatcag
     2461 taaattgact acaattaacg tctggtatga cttgcccaaa tctttcaacg cttggttctt
     2521 acccaaaaaa ctcaatacta caatagcagc aaaaagtcca aataacgacg tcgatgctcc
     2581 tgcagctact gtttcaggag tcaaccaaaa agtaaaggca ttgcccataa cacctgataa
     2641 aacataaagt aataaaaaac ggcgtgaacc ccagaggtct tcgacgattt gaccaacaaa
     2701 atatagtgtt aatccattga caaaaaaatg accaaagccg atatgaataa atatcggagt
     2761 caccaatcgc cacaactgat ctggcattgc ttttactagt aatccaaaca tacctccaac
     2821 ttgataaata gcttgagcac cagttgctaa atgtccatat accacttgca ttgctataaa
     2881 aattaagcct gttaacccta aaaggaaaat agtaatagga tatcgtttta ataaccgtgt
     2941 catctaagat aaacctccta aacagcaata tcataaatcg tttgatttgg caatcgtatt
     3001 ggcccgctga taagcttgat tgctaacaac atccactaat aaagcttgat cattttgacg
     3061 ttttaagtct acatccatag ttcgtaaaga ctagaagagt tctttgagaa tctctttttt
     3121 catcatcttc ttatcatacc ataaattagg aaaaaggttc aagactgaca agaaacta
//
LOCUS       DAUCWO010000103         3115 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUCWO010000103 DAUCWO010000000
VERSION     DAUCWO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.103"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..446
                     /gene="tilS"
                     /locus_tag="VGY20_001655"
     CDS             <1..446
                     /gene="tilS"
                     /locus_tag="VGY20_001655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ4274472.1"
                     /translation="YLKKDYYIFIDKFSFKITKIVPKTELVKEEKMLEYDSNLCYRDY
                     YFSFMPKSNEDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAE
                     RQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            451..993
                     /gene="hpt"
                     /locus_tag="VGY20_001656"
     CDS             451..993
                     /gene="hpt"
                     /locus_tag="VGY20_001656"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ4274473.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            1015..2994
                     /gene="ftsH"
                     /locus_tag="VGY20_001657"
     CDS             1015..2994
                     /gene="ftsH"
                     /locus_tag="VGY20_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ4274474.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
BASE COUNT         1081 a          439 c          632 g          963 t
ORIGIN      
        1 attatctcaa aaaagactat tatatattca ttgataaatt ctcttttaaa attacaaaaa
       61 tcgttcctaa gactgagtta gttaaagaag aaaaaatgtt agaatacgat agtaacttat
      121 gctacagaga ttattatttt tcatttatgc caaaaagtaa cgaagatcaa ggacaggtaa
      181 gtataccttt atttagttta tcgtctatta aattaagaag tcgccaatct ggagattata
      241 tttcttttgg tcacttttca aaaaaaataa gacgtttatt tatagatgaa aaattcacaa
      301 tagctgaacg tcaaaatgct atcattggag agcaagatga gcagattata tttgtattga
      361 taggtaataa aacttatttg agaaaagctt gtaaacatga tataatgttg gctaaactgt
      421 atattgataa attagaaaaa gggtgacagc atgctcgagc aagatattca aaaaattctt
      481 tactctgaaa atgatattat tcggaaaact aaaaaattag gagaacaatt aacaaaagat
      541 tatcaagaaa aaaatccgct tatgattggt gtattaaaag gatcagttcc ttttatggca
      601 gaactaatga aacacatcga tactcatgtt gaaattgatt ttatggtagt atcaagttat
      661 catggcggta catctagcag tggtgaagtt aaaattttaa aagatgtaga taccaatatt
      721 gaaggaagag atatcataat tgttgaagat attattgaca ccggtcgcac cttaaaatat
      781 ttacgagata tgtttaagta ccgtaaagct aatactatca aaattgcaac actgtttgat
      841 aaacctgaag gtcgtgttgt taaaattgaa gcagattatg tttgttacaa tattccaaat
      901 gaatttatcg taggctttgg tttagattac gcagaaaatt atcgcaatct tccttacgta
      961 ggtgtattaa aagaagaagt ctattcaaaa tagaaaaagg tcaatgttta ctgtatgaaa
     1021 aataataaaa ataatggttt tgttaaaaat tcttttatat atatattgat gattattgtt
     1081 gtcatcacag gatttcaatt ttatcttaag ggaaccagca cacaaagcca acagattagt
     1141 tattccaaat taattaaaca tcttaaggct ggagatataa aatctttaag ttatcagcct
     1201 agcggtagta ttattgaagt taaagggaaa tatgagaaac ctcaaaaagt gacagttaat
     1261 tcaggactat catttttagg tggaagagct tcaacacagg taacagagtt ctcatcttta
     1321 gtattaccta gtgataccat cttgaaagaa atgacagcag cagctgataa aaatggaaca
     1381 gagcttacag ttaagcagga aagttcaagt ggtacttgga ttaccttctt aatgagcttt
     1441 ttacctattg ttatttttgc tgctttcatg atgatgatga tgaatcaagg tggtggagga
     1501 gctcgtggtg ctatgagttt cggcaaaaat aaggcaaaat ctcaatcaaa aggtgatgtt
     1561 aaagtgagat ttacagacgt tgctggtgcc gaagaagaaa aacaagaatt agtagaagtt
     1621 gttgattttt tgaaaaatcc taaaaaatat aaatcactag gagcacggat tccagcggga
     1681 gttcttctag aggggcctcc aggtactggt aaaacattac tagctaaagc tgtagcaggt
     1741 gaagctggag tgccattttt cagtatttca ggttcagatt ttgtcgaaat gtttgtcggt
     1801 gtaggtgcta gtcgtgttcg ttctttattt gaggatgcta aaaaagctga gcgtgcgatc
     1861 atctttatcg atgaaattga cgctgtaggt cgtcgtcgtg gtgctggcat gggaggcggt
     1921 aacgatgaac gagaacagac ccttaaccaa ttactcattg aaatggatgg ttttgagggt
     1981 aatgagaaca ttatcgttat tgctgcaact aaccgtagtg atgttttaga tccagcttta
     2041 ctaagaccag gtcgttttga tcgtaaggtt ttagttggtc gccctgatgt taaaggacgt
     2101 gaagccattt tacgtgttca tgctaaaaat aaaccacttg caaatgacgt taatttgaaa
     2161 gtagtagccc aacagactcc aggttttgta ggtgctgatc ttgaaaatgt tttaaatgaa
     2221 gctgcgttag ttgctgctcg cagaaataaa attaaaattg atgctagtga tattgatgaa
     2281 gctgaagatc gtgttattgc tggaccatca aagaaagatc gcactatttc gcaaaaagag
     2341 cgagaaatgg ttgcttatca cgaagctggt catactattg ttggtttagt tttatctaat
     2401 gcgcgtgtag ttcacaaggt aactattgta cctcgtggac gagcaggtgg ttatatgatc
     2461 gctcttccaa aagaagatca aatgcttctt tctaaagaag atctgaaaga gcaattagca
     2521 ggattgatgg gtggtcgtgt tgctgaagaa attgttttta atgctcaaac atcaggtgcg
     2581 tcaaatgact ttgagcaagc tactcagata gcaagggcca tggttacgga atatggtatg
     2641 agtgaaaagc ttggaccagt tcaatatgaa ggtaaccatg cgatgatgcc aggacagatc
     2701 tctcctgaaa aagcttactc tgcccaaaca gctcaaatga ttgatgatga ggttcgtgaa
     2761 ctcttaaatc aagctcgtaa tcaagcagct gatatcatca atgagaatcg agatactcat
     2821 aaattgattg ctgaagctct tctcaaatat gaaactctag acgctgcaca aattaaatca
     2881 atttatgaaa ctggtaaaat gccagttgat ttagaaactg atgataatga agctcatgcg
     2941 ttatcttatg acgaaattaa aaataaaatg acagaatctg aatctgcaga atgagataaa
     3001 aaagctgaac ttatagatta agttcagctt ttttatcaac acaatatcct tgtaaacgtt
     3061 atgaattgta ataggtgcct aattgctaga aatgagaaga tgacatactt ttttt
//
LOCUS       DAUCWO010000104         3086 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUCWO010000104 DAUCWO010000000
VERSION     DAUCWO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.104"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..254
                     /locus_tag="VGY20_001658"
     CDS             <1..254
                     /locus_tag="VGY20_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274475.1"
                     /translation="KLFGPRKLLISTITKIEVTKSTLCLHVEDKSYLFYMRKWPKKYF
                     LDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALTRG"
     gene            complement(241..438)
                     /locus_tag="VGY20_001659"
     CDS             complement(241..438)
                     /locus_tag="VGY20_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ4274476.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            487..963
                     /locus_tag="VGY20_001660"
     CDS             487..963
                     /locus_tag="VGY20_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ4274477.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTKKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            978..1658
                     /locus_tag="VGY20_001661"
     CDS             978..1658
                     /locus_tag="VGY20_001661"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ4274478.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPIFFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWI
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    1682..1805
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1820..2350
                     /gene="infC"
                     /locus_tag="VGY20_001662"
     CDS             1820..2350
                     /gene="infC"
                     /locus_tag="VGY20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ4274479.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            2392..2589
                     /gene="rpmI"
                     /locus_tag="VGY20_001663"
     CDS             2392..2589
                     /gene="rpmI"
                     /locus_tag="VGY20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ4274480.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            2648..3007
                     /gene="rplT"
                     /locus_tag="VGY20_001664"
     CDS             2648..3007
                     /gene="rplT"
                     /locus_tag="VGY20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ4274481.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
BASE COUNT         1039 a          533 c          634 g          880 t
ORIGIN      
        1 ttaaattgtt tggtccacgt aagctactta tttctactat tactaagatt gaggtgacca
       61 aatcaacact ttgtctgcac gttgaagata aaagttatct cttttatatg cgcaaatggc
      121 ctaaaaaata ttttcttgat gccttagcgg ttaatccata cttccaagga gaagttatat
      181 taagtgataa ctttattaaa ttagactatt ttgaagttta tcagcatgat aaaaaagccc
      241 ttactcgagg gtaagagctt tagttgggca agatttgaca gctaaaatag cgtccttatc
      301 tgaaggacaa attgactgac ttgtttcaga tgagcttgaa aatgtgacga taccattgtc
      361 atgataatca aaaagtgatg aataggtttg gcatagacca caagcgatgc atttttctgg
      421 aatgatagat actttcataa ctattattct aatatgattt tgttaaatta acaagggggg
      481 acgatcatgg ctaaagaacc atgggaagaa aaaattgttg atgatactat agggacacga
      541 acacgtaaat caagaaatgc tttcattagc acgccttggt tgactgcttt attaagtgta
      601 ttctttgtca tcattgttgc tatacttttt attttcttct atacatcaaa tagcggtagt
      661 aatagacaag ctgaaacaaa tggtttttat ggagcatcca ctcataaaaa aacaaagaaa
      721 gcttctaacg ctaaaaaaac atcaagtagt tcaacaacta cagacacaac accttctagc
      781 gaagaaacac ttgcttctag tgaaggaact ggcgaaaccc ttactgtatt ggcaggtgag
      841 ggggcagctt ctattgcagc ccgtgcaggt atttctgtgg agcagctaca agcacttaat
      901 ccagagcaca tgactcaagg atattggtat gccaatccag gagatcaagt cactattaaa
      961 taattgaaga ggaaaaaatg aaagcaatta aaattgcgat tgatgggcca gcttcaagtg
     1021 gaaaaagtac agtagccaag attattgcaa aaaatcttgg ctatacttat ctagatactg
     1081 gtgctatgta tcgctcagca acttatatcg cactgacaca tggctatacg ggtaaagaag
     1141 tagcccttat tttagaggaa cttgagaaaa atcctatttt ctttaaaaaa gcaaaagatg
     1201 gctcccaatt agtttttctt ggagatgagg acgtgacctt agccattagg caaaatgatg
     1261 tgactaacaa tgtttcttgg atctcagctt tgccagaaat tagagaagag ctggttcatc
     1321 aacaaagacg cattgcacaa gctggtggca ttatcatgga tggtcgtgac attggaacgg
     1381 ttgttctccc agatgcggaa ctaaaaatct ttttagtggc ttctgtcgaa gagcgggcag
     1441 agcgccgcta taaagaaaac ctagaaaaag gaattgaatc agattttgag acgctaaaag
     1501 aagagattgc agctcgtgat tacaaggata gtcatcgtaa ggtttcacct ctaaaagcag
     1561 ctgaagatgc ccttattttt gataccacag gtgtttctat tgatggtgtg gtgcaattta
     1621 tccaagaaaa agcagaaaaa atagttgaca tgtcctagag cctatgttag aatattaaca
     1681 atgagaaaag tagaagtgag agcttctcgc cttgcgactt aggttgtctg gccctaacat
     1741 gtcgaattcc tatggaatat tatgtgtgcg ggcttgattt atcaagtccg ctttcgtttt
     1801 tctctaaaaa aataaagagg tgaagatcat agctaaaaag gatctattca ttaatgatga
     1861 aattcgcgtt cgcgaagttc gtctcgttgg tcttgaaggt gaacaattag gtattaaacc
     1921 attgtctgaa gcgcaatcat tggctgatgc ttcaaatgtt gatttggttt taatccagcc
     1981 acaagctgtt cctcctgttg ccaaacttat ggactatgga aagttcaaat ttgagtatca
     2041 aaagaaacag aaagaacaac gtaaaaaaca aagcgttgtg actgtgaaag aagttcgtct
     2101 cagtccagtt atcgataaag gtgactttga aacaaaactt cgtaacggtc gtaaattcct
     2161 tgaaaaagga aataaagtta aggtttctat tcgtttcaaa ggacgtatga ttactcataa
     2221 agagattggt gccaaggtac tagctgactt tgctgaagcg actcaagata ttgctatcat
     2281 tgagcaacga gcaaaaatgg atggacgcca aatgtttatg caacttgcac caatttcaga
     2341 caagaagtaa ttgtcacagt ttcattatat ctttaagagg agaatactaa aatgccaaaa
     2401 caaaaaacac accgcgcatc agctaaacgt tttaaacgta caggttcagg cggtttgaaa
     2461 cgcttccgcg cctttacatc acaccgtttc cacggaaaaa ctaaaaaaca acgtcgtcat
     2521 cttcgtaaag ctggtttggt tagctcagga gatttcaaac gtatcaaagc aatggttact
     2581 ggtctttaat tttaaaggca gtagacattt acgagtatta taagaattat ttgaaggaga
     2641 actattaatg gcacgtgtta aaggtggcgt tgtttcacgc aaacgtcgta aacgtatttt
     2701 aaaattagct aaaggttact atggagcaaa acacatcttg ttccgtactg caaaagagca
     2761 agtgatgaac tcttactact atgcataccg tgaccgccgt cagaaaaaac gtgatttccg
     2821 taaattatgg atcactcgta tcaatgcggc tgctcgtatg aacggtttgt catactcaca
     2881 attgatgcac ggtttgaaac ttgctgaaat cgaagtaaac cgtaaaatgc ttgctgattt
     2941 agcagtagct gatgcagcag ctttcacagc tcttgcagat gcagccaaag caaaacttgg
     3001 caaataagaa acgctatacc tagaaaataa ctttttatta cctatagaaa gttataaaga
     3061 aacaaaaatg aaggagacga tggacg
//
LOCUS       DAUCWO010000105         3068 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUCWO010000105 DAUCWO010000000
VERSION     DAUCWO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.105"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            242..976
                     /locus_tag="VGY20_001665"
     CDS             242..976
                     /locus_tag="VGY20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ4274482.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSVAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIRGLDYKWSENTSIKEALNE"
     gene            969..1955
                     /locus_tag="VGY20_001666"
     CDS             969..1955
                     /locus_tag="VGY20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ4274483.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYTYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            1965..>3068
                     /locus_tag="VGY20_001667"
     CDS             1965..>3068
                     /locus_tag="VGY20_001667"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ4274484.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVVRNDLEYSI
                     RINFPLISKFFNNDIESQLYVDKHVEKIKKYIEQKYKNISFSIDYHYYLTTTGSCIDF
                     GEEGAVGRGNKTHGIISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTP
                     CQIIMQLNIGSKLYRPTHLFIQTEESVDQERVLE"
BASE COUNT         1154 a          367 c          582 g          965 t
ORIGIN      
        1 aaattgaaaa aatatattta ctatattcac tgtaagaact atgttaacaa tgatttagga
       61 atacattatg tagaactagg taaaggaatt atcgattata aaacaatcat tcatgaaatc
      121 catgaacatt ttatagactt aatttttagt ttagaagtac atgaaaaaga aatagacaaa
      181 aaggcagaag tagtgaggaa gtcttatctg acactaaatg agtatattaa tggaggctaa
      241 aatgaatttg agtatatttg gtttaaagaa tattccttat ttgaaagaag gggatagtat
      301 agaaaagctt attgaagaaa gcattaaaac ttcaggattt tttatcgaag ataatgatgt
      361 gctttgcata gcttctaaag ttgtatctgt cgcagaaggc caggtgatgt ctttaaacga
      421 gatacaagtg agtgatgtag caaaagaaat ccacaggaat attcctcgta aagatcctag
      481 aataattgaa attatgctaa atttagtcaa tagagatctg tcacgtttag atatcaaaaa
      541 aaattacatt ggttgtcgct tggaaaatgg tttaaagttg acaagtggtg gaatcgatag
      601 aaaatcagta gatgaagtat ttcttttacc taataatccg gatgcttctg caaaaagaat
      661 ttcggaatat cttaaaaaat cacttggaaa aaatgttgca gttgttataa cagatagtga
      721 tggtagagag gataaaagag gtgctactca agtagcaatt ggtatttatg gtattcatcc
      781 tttgagaaaa acagaagtaa tagattcaca aggagagaca ataaaattcc aagaagagac
      841 tttgtgtgat atgattgcag cttgtgctgg cttagtaatg gggcaacgtg gaactggaat
      901 tccagctgtc ctcattaggg gcttggatta taaatggtct gaaaatacta gtattaagga
      961 agcgttaaat gagtagaaat caggactgta agaatattct aatcattggt tttggaaaaa
     1021 ttgggaaaat taaagcgtac aagtggctat cacgcggata tgaggtatat gtcaaagatt
     1081 tgaaattctc cagcatgaaa taccttaata caaatagtga tgtgttggat gatttatcac
     1141 gacaatattt tactattgag atttgtactc cgacaaatca ccaccttacg ctattgaaaa
     1201 ccgttataac aaggtatgtc tatacttata ttagcattga aaaaccactg tgtaacaggt
     1261 tagaagattt agaagaaatg aagtcgctag tagaaagtca cccacatcta gcaaaaaaaa
     1321 tgtttgccag tgaacaatac ttcttttcga aaattttgga aaaactgtta caagtagatt
     1381 gtttctctct agataaaatc aaggaaataa cagttaattt ttctaaagat aggattgagg
     1441 ataacgagaa tgggagattt ttagataaag agatattggg ctatggaata gagattcctc
     1501 atatcattgc tgtcatttct tatttaggaa tttcattaga agaattcaaa aaaggaatct
     1561 ttgtgaatgc tatttatata aaagacttag agaaacacga ttattcaata gaaatattat
     1621 cttgtattaa tcaaacagtg attaaaatta ttagtaattt gggaagtttt gcaataagag
     1681 agggcaagat tgaaggaggt gatgctaaaa cagtaagaag agttaccatt gatgacaaaa
     1741 caatactatt tgatccacat cctaaactcg agaggtataa gagtgaattg gtgactggga
     1801 caaatacgtt tcaaatacac gatgatatga tggacacgta tatcagtttg ttagaaggaa
     1861 atgctattcc tgaagattgc tatatcaata atgcaattga gataacaaaa ttattaatta
     1921 acttatattc agaatgtgaa aaaatttatt tatgaggtgt aactatggat ttaaaaatta
     1981 caaacggctt ttatgaccca agtcatttat catacgaagt agtagaacgc aaaggcttag
     2041 gtcaccctga taccttagca gatggaattg ctgagcaaat tgaaattgat tactcacttt
     2101 attgtttgga caagtttggt gttattccac atcataattt tgataagatt attatccgag
     2161 ggggacattc agttcaggat tttggtggaa gtgactttat tgaaccaatt aaaattattt
     2221 ttctaggtag ggcgagcaaa aaatgtttta atagttcaat tccacttttt aaaattcaga
     2281 aaaaggctgc aactaaatat ttaaatagaa ttttacctaa tttagatgtt gaaaattatg
     2341 tagaatttga gacattgacc tcagatttta ctacaaaaac taattggttc tcgcctgaag
     2401 caatagaaga tttaccggag tatctagatg taccaaaggc aaatgataca gctacaatga
     2461 taagttattg gccattaaca atttctgaag aattagcatt aatgattgaa gggtattttt
     2521 ataaattaga taagaatgaa ttaccaacgc caagatttac taaaatgggt ggtgatatta
     2581 aagtaatggt cgtaaggaac gatttagaat attctattag aataaatttt cctttaataa
     2641 gtaaattttt taacaatgat attgaaagtc agttgtatgt tgataaacac gtagaaaaaa
     2701 ttaagaaata tattgaacaa aagtataaaa acataagctt ttctattgat taccattatt
     2761 atttaactac tacaggttct tgtattgatt tcggagaaga aggtgcagtt ggtcgaggta
     2821 ataaaactca tggtatcata tcaagtttta ggccaaatac tatggaagca ccagctggaa
     2881 aaaattgtac ttattttgta ggaaaggttt ggggattttt gtctgatact attgccaaag
     2941 aaatttatga agcattcaat actccctgtc aaatcattat gcaattaaat attggatcaa
     3001 aattgtatag accaacccat ttatttattc agacagaaga aagtgttgat caagaaagag
     3061 tattagaa
//
LOCUS       DAUCWO010000106         3049 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUCWO010000106 DAUCWO010000000
VERSION     DAUCWO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.106"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            24..452
                     /locus_tag="VGY20_001668"
     CDS             24..452
                     /locus_tag="VGY20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ4274485.1"
                     /translation="MDLTLKNKDLNTLYSVLDKIKVTNMRANRGRAKLLAKVVDKINE
                     YAKDETDLIDMYAAKDKDDKFVIDEHKNIKLADPAKLDELNDLLNELADEEIVIKGGE
                     YSKRFIDFLEYLAESEDEFTAQEIIIVDSILEQYEGSKGE"
     gene            455..1066
                     /locus_tag="VGY20_001669"
     CDS             455..1066
                     /locus_tag="VGY20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ4274486.1"
                     /translation="MRNWKVTGKYPQFDSTGAVASTHIIITTEDGSVISQLVKQDLIS
                     TNDTEIIKATLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLTSGGKIDPDIYKGLLELIEPAKKGEYQAYDVFTVVDESHEEQ
                     AGEGNLVFVHVNEPFTYDKQTLEELESEDKVTVIKYADLVKQD"
     gene            1076..1372
                     /locus_tag="VGY20_001670"
     CDS             1076..1372
                     /locus_tag="VGY20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274487.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            1369..1554
                     /locus_tag="VGY20_001671"
     CDS             1369..1554
                     /locus_tag="VGY20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274488.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            1666..3000
                     /locus_tag="VGY20_001672"
     CDS             1666..3000
                     /locus_tag="VGY20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ4274489.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
BASE COUNT         1059 a          529 c          656 g          805 t
ORIGIN      
        1 attaataact tataggagaa acaatggatt taacgcttaa aaacaaagat ttaaacacac
       61 tatatagtgt actagacaaa atcaaagtca cgaacatgcg agcaaaccgc ggacgtgcta
      121 agctactcgc aaaagtagtc gataaaatca atgagtatgc aaaagatgag accgacttaa
      181 ttgatatgta tgcagctaag gataaagatg acaaatttgt cattgatgag cacaaaaaca
      241 tcaagctagc agaccccgct aaactcgacg agctcaacga cctactcaac gagctagctg
      301 atgaagaaat tgtgattaaa gggggtgagt actccaagcg atttatcgat tttttggaat
      361 atttagccga atctgaagat gagtttacag ctcaagaaat cattattgtt gacagcatct
      421 tagagcaata cgaaggaagc aaaggagaat aaccatgaga aattggaaag tgacaggaaa
      481 atacccacaa tttgacagca caggagcagt cgcaagcaca catattatta tcactactga
      541 ggatggctca gtcatctctc aacttgttaa gcaagactta atctcgacta atgacacaga
      601 gattatcaaa gctactttgg aagaatttaa aaaatctgaa tacgttgaaa ttgcaatggg
      661 cgaagccgtg caaaaggtag acgaccttga aaaaatctca caggaaaccg ctaagactgc
      721 caaaactgct caaacagctg ctggactagc taaggtgtcc gcagagcgca cgcaaaagat
      781 gattaactta caaacgatcc acatgttaac gagcggcggc aagattgatc ctgatattta
      841 caaaggtctt ttagagctaa tcgagccagc caaaaaaggt gagtatcaag cctatgatgt
      901 ctttacggta gtagacgagt cgcacgaaga gcaggcagga gaaggtaatc ttgtctttgt
      961 acacgttaac gagccattta cttatgacaa acaaacctta gaggagctag agtcagaaga
     1021 caaagtaaca gttatcaaat atgcagactt agttaagcag gattgaggtg gttagatgat
     1081 agattttgta caaattggtg ctttttgtgg cgctgctttg tctatcttgg gtgtttgggg
     1141 atttatcgtc aatcccttta aaaaagccat ggaagctaat gagtttgcca tggctcaact
     1201 caaggactca attaaggagt tagcttatga gcttaaaaat cttgatcgtg aacgtgagat
     1261 taccaaaaaa attatcgaca gacacgagga gcgtttaggt cgcgtagaag acgaagttat
     1321 tatcaacaag gagcgtatta ttacgctatt taaaaaagga gaagaaaaat gaataactgg
     1381 tttaaaaaag tagcaattaa aacaattaaa acaatggcac aaacggccgt tggtctaatc
     1441 gggtcaagcg tgttaatcac ggatattaat tggccaacaa tgttgtcagc agtgctgttg
     1501 tcaggactaa catgtatcct gatgaatgtg tcacaaatca aggaagagga ataggtcatg
     1561 cgagcaatca cacgattagc attagttata gcaatcgcaa tactgtatgt gccattatct
     1621 gtggttgctt tgatttttta tccattttta gataaggagg gataaatggc tacatatcag
     1681 gaatataaaa gtcgttcaaa tggcaatgct tacgatattg atggatcgct tggtgcgcaa
     1741 tgttgggatg gctacgcaga ctactgtaag tatctaggac tgccatacgc aaactgtaca
     1801 aatacaggat atgcaaggga tatatgggaa caacgtcaca aaaatggtat tttaaactac
     1861 tttgacgaag tagagactat gcaagctgga gatgtcgcaa tttttatggt agttgcaggt
     1921 gttacaccgt atagccatgt ggctattttt gatagtgatg caggtagtgg atacggatgg
     1981 tttttaggtc aaaaccaggg tggagcaaac ggagcatata atttagtaaa aataccatat
     2041 tcaacgactt atcctactgc atttagacca aaagttttta aaaatgcagt tactgttata
     2101 ggtaatatag gactaaataa aggcgattat tttattgatg tatcagctta tcaacaagca
     2161 gacttaaccg ctacttgtca gcaagctggc actactaaaa cgattatcaa agtatccgag
     2221 tcactcgctt ggctgtctga taggcatcag caacaagcta atactagtga ccctattggt
     2281 tattatcact ttggacgatt tggaggagat agcaacttag cgcaacgaga agcagattta
     2341 tttctgtcca atttaccaac caaaaaagtc tcttacttag tcattgatta tgaagactct
     2401 gcaagtgccg acaaagaagc taacactaat gcagttattg cgtttatgga taaaattgca
     2461 aacgctggat ataagcctgt ttattacagc tataaaccat ttacgcttaa taatattgat
     2521 tatcagcaaa ttatcgctaa gtacccaaac agcatttgga tagctggtta tccagactac
     2581 gaagtacgaa cagagccact ttgggagttc ttcccttcaa tggatggtgt gcgctggtgg
     2641 cagttcacaa gtgtaggagt agcaggtggt ttagataaaa atattgtatt attagcagat
     2701 gatagtagca aagtggatat acctaagatt gacaaaccac aaagccagct tacttttaat
     2761 caaaagctag atactaacac taaattagac aactcgaatg taccttacta cgaagcaacc
     2821 cttagaacag actattatgt agagtctaag ccaaacgcaa gtagcgctga taaagaattt
     2881 atcaaggcag gaactcgtgt aagagtctat gaaaaagtga atggatggtc acgtattaac
     2941 gcttctcagt ctgaccagtg ggtcgaagat aagtatttat ctaatgcaac acaagtataa
     3001 aataggaggt aaagctcctt tagataagac aaatgccctc gcttttgcg
//
LOCUS       DAUCWO010000107         3017 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUCWO010000107 DAUCWO010000000
VERSION     DAUCWO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.107"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(270..1142)
                     /locus_tag="VGY20_001673"
     CDS             complement(270..1142)
                     /locus_tag="VGY20_001673"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ4274490.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1366..2676
                     /locus_tag="VGY20_001674"
     CDS             1366..2676
                     /locus_tag="VGY20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ4274491.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            2754..>3017
                     /locus_tag="VGY20_001675"
     CDS             2754..>3017
                     /locus_tag="VGY20_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4274492.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQPVKMVSSTLKIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHAQFEIKKLEKEHKLLQEELALLKKF"
BASE COUNT          883 a          691 c          532 g          911 t
ORIGIN      
        1 ctcctagtta acgactttga ttgattttat tatgatgaat cgtgatttct gaaaacaaaa
       61 ttttcttcat ggatttggcg tggaactttt acaataaaaa cactaacctc tccctatcgt
      121 tttttcaggt gaatgcttgg aattggtata ataagcgcac acgaatcagg tgaaaacagg
      181 gaaagtattt gaagaggcgt ttaaactatc tgacataaaa ctcattctag aggaaagaca
      241 tcctttaaaa agttagttta ttttgcaact tagacatcaa ggtaggttaa acccttcatg
      301 gaaaaatcga gactcttagc actatgggtt aaactaccac tggagacgta atcaatcgct
      361 aaaccacgaa aacggctaat agtggtcata tcaatatttc cagaacattc aatccgagaa
      421 cgtcctgcaa ttagagtaat ggcctgttca atctgttcca atgacatatt atccaacatg
      481 ataatatcaa cacccgccgc cgcagcttct tcggcagcag caaggctttc cacttccacc
      541 tcgaccattt tcacaaaagg ggcataggca cgcgcttgag caatcgcctt ttgaacacta
      601 cctactgccg caatgtgatt gtcttttagc atgatagcat ctgataaatt aaagcgatga
      661 ttatagccac cgccaactct cacggcatat ttctcaaaaa gacgtaaatt aggagtggtt
      721 tttcgagtat caaatacctt aatgcgatca tcgcctaagg cttctacata agcagctgtc
      781 atcgaagcaa tccctgataa atgttgtaaa aaattcaagg caacgcgttc acatgttaag
      841 aggcttctca ccgagcctat gatttctaaa accaaatcgc cactagtcaa acgatcccca
      901 tccttaaatt gatgaggatt ctggaaggtc acctcggcat caaatagggt aaaaaccctt
      961 tgaaaaacgg ttagccccgc taaaacacca gcttccttgg caaaaagcga caccttggct
     1021 tggccatgat gatcaaaaat ggcattggta ctgtaatctt cggaatgaac atcttctcgc
     1081 aaggctgctt tcaatgtatc atctatttga aaaggggtta aatcagttga aatgattgac
     1141 atcactaagt cctctatttt taatatcact tgtaagcact agaggcaatt gctattaact
     1201 tccgtaggaa atcacaaacc tgaatacctc attttaaatt ccaacaagtg tcaagtcaat
     1261 tgtaattttt ttgtgtataa tagtcaagta ccaataggaa acaggtggtc ttttgagcgc
     1321 caaaattgcc aacctatttt tatattctaa ctaaaggaca attcaatgat tcatttaata
     1381 ataattagcg ccattgctct agccattgga attggttacc gcaccaaaat caatattggc
     1441 ctgctggcta ttgctttttc ttacctcatc gcaaccactc tcatgggatt aagccccaaa
     1501 gaacttcttc atttttggcc aacctcactc ttttttacca tttttagtgt ctctctcttt
     1561 tataacgttg caacaactaa cggcactctt gatgttttgg ctcaacacat tctctaccgc
     1621 acacgcaccc accctaacgc cctctacatg attttatacc tgatagcaac ccttttgtct
     1681 gctttaggtg ctggattttt cactactatg gctgtttgct gtcctctagc gattaccctc
     1741 tgtcaaaaag cggacaagca ccctttgatt ggagctcaag ccgtcaattg gggagcttca
     1801 ggaggggcta atttgataac cagtggctca ggcattgtct ttcaaggcct gtttaagcaa
     1861 atgggatggg aagagcaagc tttttcactt ggcaatcata tctttatcgt tagcattatc
     1921 tacccactca tcgtcttgct tttattatct tgttacatcc gctacagtaa gggaagaaca
     1981 aactcatcct tgactattga ccaaccacca gtcctcagca aggtccaaag acaaaccacc
     2041 ttgctcatga tcagcagcat ggttttggtt tggctttttc ccttgttgca tctcattttc
     2101 ccaaacatcg catggatagc tacttatcga caaacatttg atattggctt tgtctcaata
     2161 ctcatggtct gcctcgcctt aaggctcaaa ttagggaagc aagaagccat tttggctaag
     2221 gttccctggg ctataattat tatgctctgc ggcatgagct tactaatgtc tctagcggtc
     2281 aagtcaggtt tagtcacact gatagggcac ctcataacaa caaccattcc tcatttctgg
     2341 ttacctctgt tcttttgtgt gatagccgga gtgatgtcac tctttagttc caccctatcc
     2401 gttgtagccc caactctctt tcccatcatt gctaccatca gtgctcaaag tcctcacatt
     2461 gatattcgct tactcacaac agccactatt attggcgctc tatctactaa tatctctcct
     2521 ttttcatcag ctggttccct cattcaactc tcccttcccc atatagagga acgcagtctt
     2581 gctttcaaaa aacaaattct tctgggtgtg cccatcagct taagtttggc actcttgacc
     2641 atctggattc tcatgctgct agcttcctta agttagattt attgtctaac ttaagtttgg
     2701 acttggtata ctaaagtaga cacaaaatta agtggaaacg aggaaaagtc attatgccaa
     2761 gaaaaacctt tgataaagcc ttcaaactct ctgctgtaaa actcatcctt gaggaagagc
     2821 agcctgtaaa aatggttagt tcaactttaa aaattcatcc caatagtctt tatcgatgga
     2881 ttcaagaata tgaaaaatat ggagaaagtg cgttcccagg acatgggagc gcacttcgtc
     2941 atgctcaatt tgagataaaa aaacttgaaa aagagcacaa actgctacag gaggaattag
     3001 ctcttctaaa aaagttc
//
LOCUS       DAUCWO010000108         2937 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUCWO010000108 DAUCWO010000000
VERSION     DAUCWO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.108"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..271
                     /locus_tag="VGY20_001676"
     CDS             <1..271
                     /locus_tag="VGY20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274493.1"
                     /translation="GQRHHNTNTFDYSKFNSYIPYINYIFYRISSNPGLKEDEKVIDF
                     VKRCNNLISSDYSAESWYSERQMQDVIKKINEKYTILEDSGRESD"
     gene            640..1932
                     /gene="purB"
                     /locus_tag="VGY20_001677"
     CDS             640..1932
                     /gene="purB"
                     /locus_tag="VGY20_001677"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ4274494.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VVKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCYKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDIKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
     gene            2066..>2937
                     /locus_tag="VGY20_001678"
     CDS             2066..>2937
                     /locus_tag="VGY20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274495.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKVAVDIIQAKFEVYQTGDINFGYSILKEFLPQLKRKSIYNLNE
                     LLLIDLYLIILVVSHFSDDIFDVQFYEEITESMLKQHNNLPLEDLFLLNNILLSCADT
                     YIRLKMFGRLKETLQLSHFIMSTIQDFQKMPMYCMYEWKLSIFYLKDINRARNYFEQS
                     ILFTQMTGDTYLVQ"
BASE COUNT          965 a          471 c          612 g          889 t
ORIGIN      
        1 gggtcaacgt catcataata caaatacttt tgactattca aaatttaatt catatattcc
       61 ttatattaac tatatatttt ataggatatc gtcaaatcct ggattaaaag aagatgaaaa
      121 agtaatagat tttgttaaga ggtgtaataa tttaatttca agtgactata gtgcagaatc
      181 ttggtatagt gagcgtcaaa tgcaggatgt aattaaaaaa attaatgaaa aatacactat
      241 attggaagat agtggaaggg agtcggatta atcaaattaa atttggtaga gagtttagga
      301 aatctaactt actcattcta aatgctaagg aaattaagtt aaaaaataga aaggaacaag
      361 ggtagtcgta ttcgctaaac tgaatacagg ctacggactt ccttaactca cagaatttta
      421 agcaatacaa gaaaggagtg agggtgtttc cgagtatttg aactcgggct aacttatctc
      481 ctcaatacaa atcaaataat agaaaggaaa tgggcactag tttcactaaa ctgaacccga
      541 gcggaaagct cggaatttag ataaaccgcc taggacgcaa gcgtccattg ttggtttcct
      601 aaaattcagt cgctttctgg tcgcccttgg tatcttaaaa tgatttcacg ttattcacgc
      661 cctgagatgg cggcaatttg gacagaggaa aataaatacc gtgcttggtt ggaggttgag
      721 attttggctg acgaggcatg ggctgagttg ggtgagattc ctaaggagga tgtggttaag
      781 attcgtgaga aggcggattt cgacattgac cgcattcttg agattgagca ggacacacgt
      841 cacgatgtgg tggctttcac tcgtgcggtt tctgaaacgc ttggtgagga gcgcaagtgg
      901 gtgcactacg gtttgacgtc aactgacgtg gtggacactg cctacggtta cctctacaag
      961 caagctaacg acattatccg tcgcgatctt gagaatttca ccaatatcgt ggcagacaag
     1021 gcgcgtgagc acaaaatgac catcatgatg ggtcgtaccc acggtgttca cgccgagcca
     1081 acgactttcg gtcttaagtt ggcgacttgg tacagcgaga tgaaacgtaa tattgagcgt
     1141 tttgaacatg ctgccgcagg tgtggaagct ggtaagattt caggtgccgt tggtaacttt
     1201 gctaatatcc ctccatttgt ggaacaatat gtctgttaca aattaggcat tcgtccacaa
     1261 gagatttcaa cacaagttct tccacgtgac ctccacgcag aatattttgc agtgcttgca
     1321 agtattgcaa cttctatcga acgtatggcg acagagattc gaggtctgca aaagtcagaa
     1381 caacgtgaag ttgaagaatt ctttgccaaa ggtcagaaag gtagctctgc tatgcctcac
     1441 aaacgcaacc caatcggttc agagaacatg acagggctag cgcgcgtgat tcgtggtcac
     1501 atggtgacag cttatgagaa cgtgtcactt tggcatgagc gtgatatttc acactcatca
     1561 gctgagcgta tcatcacacc tgacacaact atcttgattg actacatgct caaccgcttt
     1621 ggcaatatcg ttaagaactt gactgtcttc ccggaaaata tgatgcgcaa tatggaatca
     1681 acttttggtt tgatttatag tcaacgtgtt atgctcaaat tgattgaaaa aggaatgaca
     1741 cgagaagaag cttatgactt agttcaacct aagacagctt attcctggga caatcaagtg
     1801 gatttcaaac cacttttaga agaagacatc aaagttacct cttgtcttac acaagaagaa
     1861 attgatgaac tatttaatcc gatttattac acaaaacgtg ttgatgatat ttttaagcgt
     1921 ttagggattt aatataaaaa aaagaaggtg attcccttct ttttttgttt aaaaaaacga
     1981 atattttctt taaaaatatt aaaaaggtta gttttttgat tagacgctgt gatataataa
     2041 agaaaacagt tgttggagag aaaccatgtt agaacatttt ggtggaaaag taaaagtgtt
     2101 aagacttgaa aagaggatta gtcgcgagga cttgtgtggg gatgagtctg aactttctgt
     2161 tcgtcaatta gcacggatag aactaggtca atccatacca agtttaagta aggttatttt
     2221 tattgcaaaa gccttaaacg ttagtgtcgg ttacttaact gatggtgctg atttagaact
     2281 acctaagcgt tacaaagaat taaaatacct tatcttaagg acaccaactt acatggatga
     2341 tggaaaatta caagtacgag aagagcagtt tgatgaaatt tttgaggatt attatgataa
     2401 attaccagag gaagagaaag ttgctgttga tattatccaa gcaaaatttg aagtttatca
     2461 aaccggagat attaatttcg gatatagtat tttaaaagaa tttctaccac agctaaaaag
     2521 aaaatctatc tataatttaa atgaattatt acttatagat ttgtatttga ttattctggt
     2581 agtttctcat ttttcagatg atatatttga tgtacagttt tatgaagaaa taacagagag
     2641 catgttaaaa caacacaata atttaccatt ggaagattta tttttactga ataatatctt
     2701 attatcttgt gcggacactt atattcgttt gaagatgttc ggtcgtctaa aagaaacatt
     2761 acaacttagc cactttataa tgtcaactat tcaagatttt caaaaaatgc ctatgtattg
     2821 tatgtatgaa tggaagctat ctatttttta tttgaaagat attaaccgag cacggaatta
     2881 ctttgaacag tctattttat ttactcaaat gacaggagat acatatttag ttcaaaa
//
LOCUS       DAUCWO010000109         2856 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUCWO010000109 DAUCWO010000000
VERSION     DAUCWO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.109"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..348)
                     /locus_tag="VGY20_001679"
     CDS             complement(<1..348)
                     /locus_tag="VGY20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HEQ4274496.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLW
                     LCYCIVLIISITIF"
     gene            complement(351..1100)
                     /locus_tag="VGY20_001680"
     CDS             complement(351..1100)
                     /locus_tag="VGY20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEQ4274497.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGENIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLI
                     ALINLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKI
                     NVYFAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPD
                     YSQIFISLFLGIAFFILLTNLFAISFKKQVK"
     gene            complement(1106..1795)
                     /locus_tag="VGY20_001681"
     CDS             complement(1106..1795)
                     /locus_tag="VGY20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ4274498.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            complement(1801..>2856)
                     /locus_tag="VGY20_001682"
     CDS             complement(1801..>2856)
                     /locus_tag="VGY20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEQ4274499.1"
                     /translation="KYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKD
                     NLGNYNTEIVVPILKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEEL
                     LNNWKKFWLNLPSELFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLP
                     QLIESCIISDIEVSSYKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVC
                     ATYLVLYYLNHFFKDKETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALD
                     RYDFFQEMDRYLMSYMKEYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKV
                     YCFAYYTLNAQQYIEWE"
BASE COUNT         1032 a          483 c          344 g          997 t
ORIGIN      
        1 aaatatagta attgaaataa ttaatactat acaatagcat aaccatagaa atactaattc
       61 tacaataaat ggcttataat tcgactgtga gtaacccaat aaatatttat attttccaga
      121 tgattcttct aaatttttta gttgaatgca cacaatactt gcaacaaaag gaagaagtac
      181 acctactatt tgaaagtagg agaataatac agtcctgctt tgtaaacctc cacgttgact
      241 ccaatagaaa aatagaagta agggaaataa tagtgcagct aaaatatgta ttagtaaata
      301 tgaagtattt ttgattttta aaaaattaga acataaatat gatttcatga ctatttcacc
      361 tgttttttga aagagatagc aaatagattg gtcagtaaga tgaaaaaagc aattcctagg
      421 aacaatgata taaaaatctg tgagtagtct ggaacaaact ccgctccaaa aggtatacta
      481 tttattctca ttcttgtcat tggctcaagc aacttcaacg accaatcata agggaacaaa
      541 taccatttat ttaagagaga aaaacttgta ccaataatta gtggagatat aatattaata
      601 atcatagcaa aatacacatt aatttttctt gatagccata aaataaacgg gatttgccat
      661 agtgtagtca accaaattaa caagatagtt aatagtatcc tacctatact aataagactt
      721 ggagtaggta ggacaaaact aattacaata agcattaagc ttaaaatcat actaattatt
      781 agatttataa gagctatcaa tagaactctg gcatattcta ccttttttaa atcaacatcc
      841 cttgataaaa aaacagaata cttattagaa tctaattcta atttatttaa caaaccagaa
      901 tttacagcaa taataacatt aatccaaata actccccact gatttactgt tgtttccgca
      961 gttaaacgta ggatattttc accacctaat gctgaaaaca ataaagataa catcacacaa
     1021 ataataggga aaataagaat caacttgcta atactcgtat tttttatttt taataaattt
     1081 gataaaaaaa tttgtaccat agcccctacc tcctcttttc aaataaaatt tgattattga
     1141 ataactcttc aagattctca gagccgtcat attctttttc cagaataatc tttccttcat
     1201 agataaatcc aataaaatca gctagatgtt caacttctga cagtatatgg cttgaaatca
     1261 taattgtcat tccttctgat ttaaatgact ctataatttc ccttaattct tgaattccaa
     1321 ttgggtccaa gccattagta ggttcgtcta atataagtag tttaggtgaa tttattaaag
     1381 agatagctat acctaaccgt tgcttcattc caagagaaaa ttgttttact ggtctcttgt
     1441 caatatttcc tagaccaact ttatttaata atggaagtat agtgctttct gaaacaccaa
     1501 gcattgttgt aactaccttc atattatcgt aagcactcaa atttttataa agtggtggtt
     1561 cttcaataat actcccgatt tttcgcagat cccgacgtga ccattctcta ccttcaaaaa
     1621 taatagaacc acttgtctta ctaactaagc ctgtcaaaat tttcataata gttgattttc
     1681 cagcaccatt tggaccgatt aaagcataca cttttccctt aggaatattc atattgacat
     1741 tattcaatat ggttctctta ccataagatt tttttagatt ttgtattttc aacatattag
     1801 ttactcccat tctatatatt gctgagcgtt tagtgtatag taagcaaaac aatacacttt
     1861 ctcttcaaac tctctatcaa tcccgtttaa tcgattcatt gatagatgta aaaaacttcc
     1921 tactaaattg aatttagtat tatagatatc atttgtacca ccaaatcgat tatactcttt
     1981 catataagac atgagatatc tatccatttc ttggaaaaaa tcatatctat caagagctga
     2041 ggtgactcca ttcaaaatct tcatatactc aataggtaaa tcaattggca aatttttgaa
     2101 atctttctta tactttccgg tataattctc aaataagaaa gagcgccttg tttctttatc
     2161 tttgaaaaag tgatttagat aatatagaac caagtaagta gcacaaacta atctttcatc
     2221 atctgaaaat tctattgtat tattaagaaa taagatagat tctttacaaa aaatttcctc
     2281 tgcataactt attaaatgag gtcctccata tctatttact tctggtttat agctactcac
     2341 ttctatgtca gagataatac aactctcaat cagttgaggt aatagatctt gaactactga
     2401 caaatacaaa gaaaagttat tttcaatcga atcacaattt atccgaattc ttatatgatc
     2461 attggtatca ttatatctca tataaaacat gttactttgg ctaaacagtt ctgatggcag
     2521 gtttagccaa aattttttcc agttatttag taattcttct tgtcgctctt ttggaccata
     2581 aaatttgaaa aatagccagt cacaaaatgg taaatatatt ttcttaactt catttttaaa
     2641 ttgaacatta tcgtttgtat tgtttattcc attagattcc ttaagaattg gaactactat
     2701 ctctgtattg tagttcccta aattatcctt aaaaatagac tctccctgct caattcctct
     2761 caaagaaaca aacctatatt tattaatgtt tttaaataaa atctctaaat cactatctat
     2821 agataaatct aacctcaatt cattatctgc atattt
//
LOCUS       DAUCWO010000110         2611 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUCWO010000110 DAUCWO010000000
VERSION     DAUCWO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.110"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            374..1006
                     /locus_tag="VGY20_001683"
     CDS             374..1006
                     /locus_tag="VGY20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274500.1"
                     /translation="MKNTLTLLLVLFAIGVTSSVRADTYGNQRIWGFRTDWEKGRDFG
                     ARPIKKGDSDIKIKTAPEAVLTVKKWENNKWTTIPQKKNTTLSSTEAQKTFSVADGAG
                     LAVFYLGNEHIANIGDKYKVTLTIDGFYLASEEWTVGESLPKDEEERDEAEIQKFIEE
                     LEQRQRESEDQKYAEELFKLSIKEEDHKTWHQRLSESIQDQWWNFKGLFQ"
     gene            complement(1100..1351)
                     /locus_tag="VGY20_001684"
     CDS             complement(1100..1351)
                     /locus_tag="VGY20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274501.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLILAALAMAVVAYAYYDPTVIPY"
     gene            complement(1709..2164)
                     /locus_tag="VGY20_001685"
     CDS             complement(1709..2164)
                     /locus_tag="VGY20_001685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274502.1"
                     /translation="MKSNLKKITAVYLALACINIFSMRPVSADDSVHPTSGSGVVDLG
                     DNVNSARSDVESLQEVGQAQDKGYDMGEKTGYQDGLRVDAPKVPKNPRPTPSTNPYSS
                     NPEAKSTYDEAYKDGYEFGYRKGWDDGHPIQATLDWLWITITSWFSRFI"
     gene            complement(2182..>2611)
                     /locus_tag="VGY20_001686"
     CDS             complement(2182..>2611)
                     /locus_tag="VGY20_001686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274503.1"
                     /translation="ESGAAYVSDYEGPYYEGRNKGKEDGYSAGNHTGVPDAPGPHTSP
                     FDPYSSDKRSSYLYRSGYEEGYTQGYYEGWHNANDSVTEGQDNLEGSSDDSNDSKGLE
                     SQVDSENPMDMSKLSILDLVYGMVKDFWGYMLDVLTGNAK"
BASE COUNT          836 a          529 c          499 g          747 t
ORIGIN      
        1 taaaagacac cccgtaaggt gccttttttc ttgcctgtgg acgccttttt ttatcccctt
       61 tttagtggga cttgtgatgg cctttaaaac aggtctatga aacgatttgt gtgataaaat
      121 cctcaatgct actagataag aaaccctatg aaatgcagta actatctagg atcgagaatc
      181 tcttcaaatg tcatattgtt cttctttatg gacgatatcc gttgcgctaa taggaacttt
      241 agtcagtctg tctgccccta ccaagaatag tcgcgccatt tcccaaccac aagacataaa
      301 ctccctatct ttttagcgca agaattggta taataagcct tgtcttaaaa aatgagcaaa
      361 aggggataaa aaaatgaaaa atacattaac cttgctactg gtactctttg ccatcggggt
      421 aactagtagc gtcagagcgg atacgtatgg caatcaaaga atatggggat ttagaacaga
      481 ttgggaaaaa ggacgagatt ttggtgctcg ccctattaaa aaaggggata gtgatattaa
      541 aattaaaaca gcaccagaag cagtcttaac tgtaaaaaag tgggaaaata ataaatggac
      601 aactattcca caaaaaaaga acaccacttt aagtagtact gaagcacaga aaactttttc
      661 tgttgctgac ggtgcaggtt tagcagtgtt ttatctaggg aatgagcata tagcaaatat
      721 aggcgacaaa tacaaagtaa cactcactat tgatggtttt tacttagcta gtgaagagtg
      781 gaccgtaggg gaatcgctcc caaaagatga agaagaaagg gacgaagctg aaattcaaaa
      841 atttattgaa gagttagagc aaagacaacg agaatcagaa gaccaaaaat acgctgagga
      901 actattcaaa ctttctataa aagaagaaga ccataaaact tggcatcagc ggttaagtga
      961 gagcatccaa gatcagtggt ggaactttaa gggactgttt cagtgagggt ttgctaggct
     1021 atgaggttag ctaaagtaca aaaagcacct gtcaggggtg ctttttgtga tgttgattgc
     1081 gtgcaaagag gggggaaggt tagtagggga tgacggttgg gtcatagtag gcataggcca
     1141 ctactgccat agctagagca gctaagataa gcccagctag cgaccaaacc ttttgcctgc
     1201 ctttgttttg caccagaccg ataagagcta gactacttgc tacaagcggc aagtagagat
     1261 aactataagt agcaaaaaag aagatatgat caagcaggca gacaaaactc accaccatgc
     1321 ttaggacggg caatactttc ttttctggca taaaagctcc tttctcaaca cacggatcac
     1381 taaaacccaa aaaagccacc cctatcagaa gtggcctgac taccaaaggg actatcccaa
     1441 ttatagcaga aaattgtttt aaaatagcgt gatgttaacc aaataatttt tattgtttac
     1501 tataattaat tttgctcact gtcagctgga aagaatgatg ataggaaatc ccatagccag
     1561 tctagtgcag actctaatgc cgcttgtatc ggatgatttg tttcacgcca tccctgatag
     1621 tatcctaatt gataaccatc actatagccg tctccttagc ttttttatcc agattttgct
     1681 catccatttc aagagcatct tgcttagatt aaataaaacg tgagaaccaa gaagttatcg
     1741 ttatccataa ccagtctaaa gttgcttgta ttggatggcc atcatcccac cccttacgat
     1801 atccaaactc atatccgtct ttatacgcct catcgtacgt agatttcgcc tctggattac
     1861 tactataagg attagtagag ggtgtaggtc tcggattttt cggtacttta ggagcatcta
     1921 cccgtaatcc atcctgataa ccagtttttt cacccatatc ataaccttta tcttgtgctt
     1981 gtccaacttc ttgcaatgat tctacatccg acctagctga atttacatta tcacctaagt
     2041 caacaactcc tgatcctgaa gtaggatgaa cggaatcatc agccgatact ggtctcatag
     2101 aaaagatatt tatacatgct aaagctaaat aaacagcagt gattttcttt aaatttgatt
     2161 tcattaaatt aacctatttc cttatttagc atttcctgtg agtacatcta acatatatcc
     2221 ccaaaaatct ttaaccattc cataaactag atctaaaata gaaagttttg acatatccat
     2281 gggattttca ctatctactt gagattctaa cccttttgag tcgtttgagt catcagaaga
     2341 accttctaga ttatcctgac cctcagtaac agaatcattg gcgttatgcc atccctcata
     2401 atatccctga gtatatcctt cctcataacc agatctatat agatagctgc ttcgtttatc
     2461 agagctatag gggtcaaaag gagaagtatg tggtccagga gcatcaggga cacctgtgtg
     2521 attgccagca ctataaccgt cttctttacc cttgttccta ccctcataat atggaccttc
     2581 ataatcactt acataagcag ctcctgattc g
//
LOCUS       DAUCWO010000111         2583 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUCWO010000111 DAUCWO010000000
VERSION     DAUCWO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.111"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(118..1080)
                     /locus_tag="VGY20_001687"
     CDS             complement(118..1080)
                     /locus_tag="VGY20_001687"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ4274504.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            complement(1333..2529)
                     /locus_tag="VGY20_001688"
     CDS             complement(1333..2529)
                     /locus_tag="VGY20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4274505.1"
                     /translation="MKKRILSAVLVSGVTLGAATVVSADNFDAKISDANSRISALTAE
                     QQSAQNQVNALQAQVSSLQAEQDKLTAKNSELEELSKGFEQEIQSLTNQIIARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVITRLVAINKAVSANAKMLEQQKADKASLEE
                     KQAANQEAINTIAANMSAIKENQNALRTQQADLEVAKVNLAIQLASAEDEKASLLNQQ
                     AAAQQAAAEALAAQQAAQAKAREEAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPASPSARPQVSYSSVNNYPVGQCTWGAKSLAPWAGNNWGNGGQWAASAQAAGYS
                     VGSTPMVGAIAVWNDGGYGHVAVVVEVQSSSNIRVMESNYSGKQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT          782 a          534 c          443 g          824 t
ORIGIN      
        1 tatacccaaa atcctttaga attgcatagt tcatcccaca ataattaata ccacattacc
       61 cttacatttt attcaagtca aaaagcccga gatctattat ctcaggcttc gttaagctta
      121 attgcccatc tcaaataaag gtgacaacgg acgtttttca tgaatacgaa taatcgcttc
      181 tgcaaccaaa tcagcaattg aaatttgttc aattttatca ataagacgtt cttttggcag
      241 ataaatagta tcaagaacaa ttagtttttc gatagccgaa cgttgaatat tatcaagtgc
      301 tggtccagaa agaacgggat gcgtgcatga cgcatacaca gctgttgccc ccgcttcagc
      361 aagcgcatct gctgcatggc aaatagtccc tgcggtatca atcatatcgt caatcaagat
      421 acatttcttg cccgaaacat ttccgataat attcatcact tcactggtgt tcatcttatc
      481 cacactacga cgcttatcga taattgcaat tggtgtttgg aggaattgtg ctaatttacg
      541 cgcacgagtc acaccaccat ggtcaggact aacaacgaca acatcttcac ccactaaacc
      601 atgacgatca aaatagtctg caattaaagg tgcacccatc aagtgatcaa ctggaatatc
      661 aaagaaccct tgaatttgag cagcatgtaa atcaaccgtt aataagcgat ctacaccagc
      721 aacttctaac atattagcaa ctaattttga agtaataggt tcacgagaac gcgctttacg
      781 gtcttggcgt gcatagccgt agtaaggcat cacaactgaa attttttcag cactagcacg
      841 ctttaaagca tcgaccataa tcaaaatttc cattaaattg tcattgacag gtgagctagt
      901 tgattgcaaa ataaagacat gatgaccacg aattgattct tcaatattta cttggatttc
      961 accatctgaa aactggcgaa ccgtagactt tccaagttga agacccattg cagacgcaac
     1021 tttttctgct aattccttgt ttgacgataa agcaaacaat tttaaatcag aataagacat
     1081 tttttcctcc aaagttttaa tcctatacca ttttaacgct tttcaagtga attatcaaat
     1141 gaaattgaaa aaccgttaaa gaatgtatta aatatacctt ttatcgactt tatatacttc
     1201 tcttatatta tacataagcg attggccaaa caactctctt ataaattaac catatcgtca
     1261 atactctcag aactattcca caataggagt aatcaaaaag gttagaacaa ctgttctaac
     1321 ctaaataata tgttagtgtg gataaataaa tgtaacacct gttggattaa accaaccacg
     1381 gtggtcagca atatactgtt taccactgta gtttgattcc atcactcgaa tatttgatga
     1441 actttgaact tcaacaacta ctgcgacgtg accataaccg ccatcattcc aaaccgcaat
     1501 agctcctacc ataggtgtag atcctacaga gtagcccgct gcttgagcgc ttgcagccca
     1561 ttgtccaccg ttaccccaat tgtttcctgc ccatggagct aatgatttcg caccccatgt
     1621 acattgtcct actgggtaat tattaacaga actatatgaa acttgtggtc ttgcagaagg
     1681 agatgccgga gccgtgagtg cagctggttc aatagcatta ctactttgcg ctgccggagt
     1741 agcttgtggt gctggagtaa tagcagattt tgctgcttca acagatgctg cttgttgtgc
     1801 agcttcctct cgagctttag cttgtgctgc ttgttgtgct gctaaagctt ctgctgctgc
     1861 ttgttgcgct gccgcctgtt gattaagtaa acttgctttt tcatcttcag cagaagccaa
     1921 ttgaatagct aaattgactt tagcaacttc caaatctgct tgttgtgtac gcaaagcatt
     1981 ttgattttct ttaatggctg acatatttgc agcaattgta ttaatagctt cttggttagc
     2041 cgcttgtttt tcttcaagtg aagctttatc agctttttgt tgctctagca tcttagcatt
     2101 agcagagact gctttgttaa tagcaactaa acgagtaatc acgtcagaaa ttgatttaga
     2161 attcaaaagt gcattaatgt aaccagaagt ttcattgttt ttataagcac tacgagcttg
     2221 attttttaat ttttcattac gagcaataat ttggtttgta agagattgaa tttcttgttc
     2281 gaatcctttt gaaagttctt caagttctga attttttgct gttaatttat cttgttcagc
     2341 ttgtaatgaa ctaacttgtg cctgtaatgc attaacttga ttctgagcag attgctgttc
     2401 tgcagttaag gctgagattc ttgagttagc atcagagatt ttggcatcaa aattatccgc
     2461 gctaactaca gtagctgctc caagggttac accacttaca agaacggctg atagaattct
     2521 ttttttcata ataaaaaaac tactcctttt cgataagaca tcttatattt taccaaaaaa
     2581 aaa
//
LOCUS       DAUCWO010000112         2533 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUCWO010000112 DAUCWO010000000
VERSION     DAUCWO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.112"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            31..798
                     /locus_tag="VGY20_001689"
     CDS             31..798
                     /locus_tag="VGY20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4274506.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
     gene            1189..1350
                     /locus_tag="VGY20_001690"
     CDS             1189..1350
                     /locus_tag="VGY20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274507.1"
                     /translation="MSINKKQVFLTLCGISISVTGIDMLLSGEYLRGVIKLILAFGIF
                     VIFSKDRDE"
     gene            1611..2465
                     /gene="dinD"
                     /locus_tag="VGY20_001691"
     CDS             1611..2465
                     /gene="dinD"
                     /locus_tag="VGY20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEQ4274508.1"
                     /translation="MNGLQHYDELAFENIKHIDENGVEFWYARELQTVLEYAKWDNFL
                     NVIERAKIACENTGNPVAMNFPEVSKIVQLPLGDREIKDYKLSRYACYLIVQNGDPRK
                     EVIALGQSYFAIKTRQQELADNFNKLDEEHKRLAIRQEMKEHNKSLAEAAKMSGVTNY
                     GKFQNFGYKGLYGGMSMQDIHDRKELEKGQSILDYMGSAELAANLFRATQTDEVLRNR
                     QIHDENLANDTHFNVGRTIRKTMQKLGTTMPENLPTPQESIQDLKKKHKQLDKQPDDN
                     QLSLFDDM"
BASE COUNT          899 a          454 c          443 g          737 t
ORIGIN      
        1 ttcagataca aaaagaaagg ggtaagagaa atggctaaaa atagtcccca agatttaata
       61 aatagagaaa ttttctcaac aaatctcaac atgcttatgg ctaaaaagaa tatcaaacag
      121 atagatattc acaacaaact aggaatacct aagagtacga taactggcta tgttaaaggt
      181 cgttcactcc caactgctgg aaacgttcaa aagctggcgg acttcttcgg agttctaaaa
      241 tcagacattg acccccgttt tgattctaat aatattgaaa caaatagtaa tattatccca
      301 tcaaccctac aaaaagtaac atctacttta tctcagctag aacacaagcg acaattaaac
      361 gtccttgatt atgctgaaac acaattagaa caacaaaaca cagtagaaga accacaagcc
      421 acctactaca cttacaatta ctacgaccac gcagcttcag ctggtacagg tcagtatcta
      481 aatgatgtac aagtagaaac aattgaatta ccagtcgatt acgacgctga ttttgtcata
      541 ccggtttatg gcgattctat ggaacccgaa taccattctg gggactatgt atttatcaaa
      601 ctatctatta acctgtcaga tggtgatata ggagtttttg agtattacgg tgatgcttat
      661 atcaaacaac ttgttataaa cgattctgga gcatttctgc atagtctgaa cgacaagtat
      721 gacgatatac tcatagatag agatagtgat ttccgtatta tcggagaagt tattgggagt
      781 tttacatcta aatcatgact atctgatacc cacgcgccga attccactat tttccactaa
      841 ttttggttgc tttaccttta aggactcgca ttttgctctt taagacagcc gaaaagtccg
      901 attttctgaa tactatatgc taaaaatgcc aactgatttt agaagctgtc acaacggaaa
      961 aagtaaatta ataaacgacc gatatatcaa gttctttaag tgaatttacc gagcgtttta
     1021 gaactattga aataggttga cgtattatgt cggtacatac caacattttc cagcattccg
     1081 aaatgcgacc atgtgttcgt gtttgggagt agtaaccttc tattctctaa gttcgctcga
     1141 agttcagcat tatgcgcgtg gttgtatttc aataaaagga gattttctat gagtataaac
     1201 aaaaagcaag tatttttgac actgtgtggt atttctattt cagtgacagg tattgatatg
     1261 cttctatctg gtgagtatct aagaggagtt atcaagttga ttttagcttt tggaattttt
     1321 gtcatattta gcaaagatag agatgaatag cccatagttt ctattttaag ttcgaaaatg
     1381 cccactataa tgtgttaaaa cagacatgaa atactctagc agtgtaaaac tatactccac
     1441 tgctttaagt ctaaaaatga gctaccagag tagaaaaaac gacttataac taaaactatt
     1501 atcttatctg ttaagcagtt gcataaaata tcataaaaat atacataaaa ttatttttat
     1561 tcatttttaa ctcaccgtac cttgaccgtt agaccataga aaggaaaagc atgaacggat
     1621 tacaacatta tgacgaatta gctttcgaaa atattaaaca catagatgaa aacggtgtag
     1681 agttttggta tgcccgtgaa ttacaaactg ttttagagta tgctaaatgg gacaattttt
     1741 taaacgttat tgagcgggct aaaatagctt gtgaaaacac tggaaacccc gtcgctatga
     1801 attttcccga ggtcagcaaa attgtacagc tccctctagg agatagggag attaaagatt
     1861 ataaactatc tcgctatgcc tgctatttga ttgtccaaaa tggtgaccct agaaaagaag
     1921 tcattgcact gggtcaatca tattttgcta ttaaaacccg tcagcaagaa ctagctgata
     1981 atttcaacaa actcgatgaa gagcacaaac gtttagcgat tcgccaagag atgaaggaac
     2041 ataataaatc attagctgaa gctgctaaaa tgtcgggtgt cactaactac ggcaagtttc
     2101 aaaactttgg atataaaggc ctttatggtg gaatgtctat gcaagatatt catgatagaa
     2161 aagaactaga aaaagggcaa agtattcttg attatatggg tagtgctgaa ttggccgcta
     2221 acttattccg tgctactcaa accgatgaag tgttaagaaa tcggcagata cacgatgaaa
     2281 atctagctaa cgatacacac tttaacgtag gtagaacaat cagaaaaaca atgcagaaac
     2341 taggcacaac tatgcccgaa aatctcccta cacctcaaga gagtatccaa gatttgaaaa
     2401 agaaacataa acaactagat aaacaaccag acgataatca actttcactt tttgatgata
     2461 tgtaaaaaat aggcaataat atcctcgtat agctatttca actctcaatc atataatttt
     2521 atcaacttac ccc
//
LOCUS       DAUCWO010000113         2217 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUCWO010000113 DAUCWO010000000
VERSION     DAUCWO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.113"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            73..753
                     /gene="radC"
                     /locus_tag="VGY20_001692"
     CDS             73..753
                     /gene="radC"
                     /locus_tag="VGY20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ4274509.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(755..1450)
                     /locus_tag="VGY20_001693"
     CDS             complement(755..1450)
                     /locus_tag="VGY20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ4274510.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEGKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(1460..2104)
                     /locus_tag="VGY20_001694"
     CDS             complement(1460..2104)
                     /locus_tag="VGY20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ4274511.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT          737 a          376 c          414 g          690 t
ORIGIN      
        1 attcaaaaaa aacaagtttc ctaaaaggaa cttgtttttt tgtcgaataa aaagatggag
       61 gtaatatgat aaatgtattc tataaaatgt gatgataata aagccatgcc aagagaacgt
      121 ttgatgcgac taggagcaga gtctctaagt aatcaagaat tattagcaat tttattacga
      181 acaggtaata aagaaaagca tgtcttagag ctgtcatcct atcttttatc gcatttagac
      241 agtctggcag attttaaaaa gatgtctttg caagaattac aacatttggc aggtatagga
      301 aaagttaaag cgattgaaat taaagctatg attgagttgg tttcccgaat tttagcgaca
      361 gataagacat taactgatag cgtattaacc agtgttcagg tcgctgaaaa aatgatggca
      421 gctttaggag ataaaaaaca agagcattta gtcgtattgt atttagataa tcaaaatcgt
      481 attattgaag aaaaaactat ttttattggg actgtccgac gttcacttgc agaaccaaga
      541 gaaattttat actatgcctg taaaaatatg gcgactagtc tcattgttat tcataatcat
      601 ccttcaggaa atattgaacc tagttctaac gattattgct ttactgaaaa aataaaacga
      661 tcatgtgaag atttaggcat tatctgtcta gatcacatta tcgttagcta taaagattat
      721 tatagttttc gagaaaaatc aacccttttt taagtcagaa gtcattgacg aataagcgga
      781 acaattgtag atcttcatct cgaacacctt ggagaagttc aggatgccac tgaacaccta
      841 aaaaaggaat ggcatcgttg gttgatatga ctgcttcgat ggtaccatca cgtggatctc
      901 tagcgattac ttttaaatcc ttagcaacag tttttaaact ttggcgatga aaagaattaa
      961 ttaatgtctt atgaccataa ataggatata agatagagtc tggctcaata atcatttcgt
     1021 gtgataagaa atcagaaggc gcttcttgcc aatgtgagtc aatgtgttgg ttaagatttc
     1081 ctcctaaggc aacattcatt aactgtgtac cacgacaaat ccctaagatt ggttttttta
     1141 atgttatagc ctccttgatg atagctagct caaatgtgtc tcgctctggt gaaaaatcat
     1201 catcaaaagc agccttccct tcttgataat attttgggtc aacgttttgc ccaccaatta
     1261 aaatgatttt atcaaccata gatacataag ttttagcggc ggcttcatca ccaatgggta
     1321 aaagaagcgg cagcccccca gattgagtga ctgcctgtac aaatccagta ggtgcatatg
     1381 accatgggag attatccaaa gccatattta gtctttgatt tgcagttatt ccaataatcg
     1441 gtttggtcat tatacctcct taatctagat gattttgatt cataaaatag agtagtgtct
     1501 ggagctcact ggtcaaatcg acagattgaa caataactcc tttaggaact tgtaggtgaa
     1561 caggagcaaa actcaagatt cctttgattc ctgcttcaat aagttgatca gtaacttctt
     1621 gagcatgaat gctgggaacc gttaagatag ccgtttcaat gtcagtgttc gcaatccgtt
     1681 ctttgacaga tgagatacca tggactggaa tattatcagc tgttttagta ccaacaagag
     1741 cgttatcatc ggtatcaaaa cccatggcaa tttgcatctt attacgatca tggaagcggt
     1801 aatgtaatag agcacgccct atattaccgc atccaactaa gataacgttt gttgtagagt
     1861 gatcattaag caagtcagca aaaaaattca tcaatttggt aacatcatag ccaaacccac
     1921 gacgccccaa ttctccaaaa taagaaaagt cacggcgtac agtagcagag tcaattccca
     1981 ttgcatctgc aatttgttta gaactggctt tttcaacttg gtcagcatgg aatcgtttaa
     2041 aaatacggta ataaagtgat aagcgctttg cagtcgcttt tggaatagat ttgtcaataa
     2101 ccactatttt actccttgta gatttctcgt taaatatgtc tgtctttatt gtaacaaaaa
     2161 gattgtgaaa aatgcaacat tttgattgtt tttgtatgag ttaaaaaagc taaaatt
//
LOCUS       DAUCWO010000114         2053 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUCWO010000114 DAUCWO010000000
VERSION     DAUCWO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.114"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..988
                     /locus_tag="VGY20_001695"
     CDS             <1..988
                     /locus_tag="VGY20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ4274512.1"
                     /translation="SNVTVSHDVLPNIKAIKNSTTAGLMQSDSYGGGTANMEFQTLTS
                     LPFYNFSSSVSVLYSEVFPKMAKPHTISEFYQGKNRIAMHPASANNFNRKTVYSNLGF
                     SKFLALSGSKDKFKNIENVGLLTSDKTVYNNILSLINPSESQFFSVITMQNHIPWSSD
                     YPEEIVAEGKNFTEEENHNLTSYARLLSFTDKETRAFLEKLTQINKPITVVFYGDHLP
                     GLYPDSAFNKHIENKYLTDYFIWSNGTNEKKNHPLINSSDFTAALFEHTDSKVSPYYA
                     LLTEVLNKASVDKSPDSPEVKAIQNDLKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            1167..1862
                     /locus_tag="VGY20_001696"
     CDS             1167..1862
                     /locus_tag="VGY20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4274513.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1864..>2053
                     /locus_tag="VGY20_001697"
     CDS             1864..>2053
                     /locus_tag="VGY20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HEQ4274514.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGI"
BASE COUNT          684 a          311 c          364 g          694 t
ORIGIN      
        1 atcaaatgtt actgttagcc acgatgtttt acctaatatc aaggcaatca aaaatagcac
       61 aactgcggga ctcatgcagt cagactccta cgggggtgga acggctaaca tggagtttca
      121 aacgttaaca agcttacctt tttataattt ttcttcttca gtatctgttc tttattcaga
      181 agtctttcct aaaatggcca aacctcatac gattagtgag ttttaccaag gaaaaaatcg
      241 tattgcgatg catcctgcta gtgctaacaa ttttaataga aaaacagttt atagtaattt
      301 aggtttttcc aaattcttag ctctatcggg ttctaaggat aagtttaaga acattgaaaa
      361 tgtcggttta ttgactagcg ataaaactgt ctataataat attttatctt taattaatcc
      421 tagtgaaagc caattttttt cagttattac aatgcaaaat catattcctt ggtcatccga
      481 ttatcctgaa gaaattgttg ctgaaggaaa aaatttcacg gaagaagaaa atcacaacct
      541 aacaagttat gctcggttat tatcgtttac tgataaggaa acaagagcat ttttagaaaa
      601 attaacacaa attaacaagc ctatcacagt ggtgttttac ggagatcatt tacccggttt
      661 atatcctgat agtgctttta acaagcatat tgaaaataaa taccttactg attattttat
      721 ttggagtaat ggtactaacg agaaaaaaaa tcatccgctt atcaactcaa gtgattttac
      781 tgcagcttta tttgagcata ctgattcaaa agtatcacct tactatgctt tgttaacaga
      841 ggtactgaat aaagctagtg tcgataaatc accagatagt cctgaagtta aagctattca
      901 gaatgattta aaaaatatcc aatacgatgt gactatagga aaaggttacc ttttgaaaca
      961 caaaactttt tttaagatat cacgttaaga aaaatgctag gctgtccgag tataggcaag
     1021 catcaaggtt tggtaatttg ctcaaatgat tgtcaacaga gtgtctagga ccaggtttaa
     1081 aagattataa ttcttaaggt cttattcgtt tataaaaaat gaatactctt ttaaaatctt
     1141 aattgaaatg aataggagaa gttttcgtga aaaagttaat cattattcct gcttacaatg
     1201 aaagcagtaa tattgtcaat actatacgta ctattgaatc agatgccccg gattttgact
     1261 atatcattat tgatgattgc tcaacggata atacgttagc aatatgtcaa aaacaggggt
     1321 tcaatgttat ttctttgccc attaacctgg gaattggcgg tgcggtgcaa actggctatc
     1381 gttatgcaca aagatgtgga tatgacgttg cagttcaagt agatggagat ggtcagcaca
     1441 atccatgcta tttggaaaaa atggttgagg tattagttca atcttcagta aatatggtaa
     1501 ttggatcacg atttatcaca aaagaagggt ttcagtcatc atttgctcga cgtatcggca
     1561 taaagtattt tacttggctt attgcgctac taacaggaaa aaaaataaca gatgcaacgt
     1621 caggtctaag gttaattgac cgttcattga ttgaacgttt tgctaatcat tatccagatg
     1681 attatcctga acctgaaaca gttgttgatg tattggttag tcattttaaa gtgaaagaaa
     1741 ttcctgttgt aatgaatgag cgacaaggcg gtgtgtcatc tatctcgttg acgaaatcgg
     1801 tctattatat gattaaagtg actttagcta ttttagttgt tagattgaaa ggaaatcgtt
     1861 aaaatgacgg taacagccag tttatttata agttttattg tattaacctt tgttttcttt
     1921 ttgattaact taatcaaaaa agataaacta gcgattaagt actctttatt gtggtttatt
     1981 ttagctttat taattttact atttacgtgg ttacctaaca tattaaataa aatgtctcac
     2041 ttcctgggta ttc
//
LOCUS       DAUCWO010000115         2013 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUCWO010000115 DAUCWO010000000
VERSION     DAUCWO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.115"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            79..1911
                     /gene="lepA"
                     /locus_tag="VGY20_001698"
     CDS             79..1911
                     /gene="lepA"
                     /locus_tag="VGY20_001698"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ4274515.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDTKK"
BASE COUNT          578 a          376 c          478 g          581 t
ORIGIN      
        1 aatccgtaag aaaagatagg caaatgccta ccttttttgt tataatagaa ggaaagacta
       61 aatgtatagg aaaatagaat gaacagtcaa gatttaaaaa aacgtcagga gaagattcgt
      121 aatttctcca ttatcgcaca tattgaccat ggcaaatcta ctctcgctga ccgcattttg
      181 gaaaagacag agacggtttc gtctcgtgaa atgcaggctc agttattgga ctccatggat
      241 ttggagcgtg agcgtgggat taccatcaag ttaaacgcca ttgagcttaa ttacacggct
      301 aaagatgggg aaacctacat tttccacctt attgataccc cagggcatgt ggactttact
      361 tatgaagtat cgcgttcgtt ggcagcttgt gagggagcga ttctagttgt ggatgcagcg
      421 cagggtattg aagcacagac ccttgccaat gtttacctag cccttgataa tgatttggaa
      481 attttaccgg ttatcaataa aattgatttg cctgctgctg accctgagag agtccgccat
      541 gaagtagaag atgtcattgg acttgatgct tctgaggctg ttctggcatc agccaaggct
      601 ggtattggga tcgaagagat tcttgagcaa attgttgaga aggttcctgc tcctacgggt
      661 gatgtggatg cgcctttaca ggccttgatt tttgactctg tgtatgatgc ttaccgaggc
      721 gttatcctgc aagttcggat tgtgaacggt attgtcaagc ctggcgataa aatccagatg
      781 atgtcgaatg gaaaaacttt tgatgttaca gaagttggca ttttcactcc taaggcagtt
      841 ggacgagatt tccttgcaac gggagatgtt ggttatgttg cggcttctat caaaacggta
      901 gcagataccc gtgtggggga tacggtgact ttagctaata accctgctaa ggaagccttg
      961 catggctaca agcagatgaa cccgatggtc tttgcaggga tttatccgat tgaatctaat
     1021 aaatacaatg acttgcgtga agcgcttgaa aaattacaac tgaacgatgc cagtttacag
     1081 tttgaacccg aaacgtcaca agcgcttggc tttggttttc gatgcggctt tttaggcttg
     1141 cttcacatgg atgtgattca agaacgtttg gaacgtgaat ttaacattga tttgatcatg
     1201 acagcaccgt ccgtggtgta tcacgttcac acaacagatg aggacatgat tgaagtgtct
     1261 aacccttcag aatttccaga cccaacccgg gtggctttta ttgaagagcc ttatgttaaa
     1321 gcgcaaatca tggtgccaca agagttcgta ggggctgtta tggaattatc gcagcgcaag
     1381 cgtggtgatt ttgtgaccat ggattacatt gatgataacc gtgtcaatgt gatttaccaa
     1441 atcccgctgg ctgaaattgt ctttgatttc tttgataaat tgaaatcctc aacgcgaggc
     1501 tatgcgagtt ttgattatga catgtcagaa taccgcaggt cacaattggt gaaaatggac
     1561 attcttttga acggtgataa ggtcgatgcg ctcagcttta ttgttcataa agaatttgct
     1621 tatgaacgcg ggaaaattat tgttgataaa ttgaaaaaaa tcattccacg tcaacagttt
     1681 gaagtgccga tccaagccgc tattggtcaa aaaattgttg cgcgttcaga catcaaggcc
     1741 cttcgtaaaa acgttttagc gaagtgttat ggcggtgacg tgtctcgtaa acgtaaattg
     1801 ctcgaaaaac aaaaggctgg taaaaaacgg atgaaggcta ttggttctgt cgaagtccct
     1861 caagaagcct tcttgagtgt tctttcaatg gatgacgata ccaagaaata accaagctta
     1921 accgcttagt cttcagagga gaactcttgt gggtctacat gaccaaaaaa ttgattatct
     1981 cataaaaaaa gaactttaca atctctttca gat
//
LOCUS       DAUCWO010000116         1937 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUCWO010000116 DAUCWO010000000
VERSION     DAUCWO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.116"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            408..1663
                     /locus_tag="VGY20_001699"
                     /pseudo
     CDS             408..1663
                     /locus_tag="VGY20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          646 a          431 c          328 g          532 t
ORIGIN      
        1 attacctcag aaagtgcttg cttgggactg ttagtccgcc tttttgcgct taggagttgt
       61 ttttgaaagc ctttgatgtt ttgtgttaga ccttactagc aactacagtg aaagcgtgga
      121 catttgcgtc tattttaata gggaatggct gtcggttcag attatactgt tactgctact
      181 acaagcccag ccagcgaaca cttgcttttg ttttatacaa gacctacaat ggctagacta
      241 cttgctacaa gcggcaagta gagataacta taagtagcaa aaaagggctc tataatatct
      301 gtagtgggta actcccctac agatattata agactttttg gatgtacaca aaaaatccca
      361 tatgacttat aatgcaaagc gaccaaacca tcattagaaa gaattatatg agacaactct
      421 atcatactac agaattgatt ggaattaaag ataaacacat caccttgaac aaggtttttc
      481 aacgcgagac acctatcgaa gtcttcgcca cacttgacta tcccccacct aagtgtaaac
      541 actgtaaagg agttcagatt aaatacgact cccagaaaca ttctaaaatc ccctttatcg
      601 aaattggtgg tttccctagt ctcattcgtt taaaaaggag aagatctcaa tgcaagtcct
      661 gtcgaaaagt ggctgtagcg gaaacaagtc tggttcaaaa aaaactgtca gatctctgag
      721 cacctcagac aaaaagtagc acaaaccttg gttaatcgag gcgctctcac taacatcgct
      781 caacagtcgg ctagatcatc ctctaccgtc taccgaaaac tcaaccagtt tgtaatcaag
      841 gaagacttct ctaaactact cgaggtgttg tcttgggatg gatttttcta tcaaaagggc
      901 gaactcgcct ttatcgctca agattttgag tccaaaaaaa tcatctccat actggacagc
      961 cggcgacaaa caaccattcg aaatcacttc tttagatact ctaaagaggc tagaaacagt
     1021 gtcaaaatcg ttaccgttga tatgtcagga agctatattt cgctcattaa aaagctattt
     1081 cctcaagcta acatcgtcct agatcgtttc catattgttc agcacatgaa cagagcgctc
     1141 agtcaaaccc ggattcacat catgaagcaa tatgacaaaa agtctctgga atacagagtt
     1201 cttaaatact actgtcaact cattcaaaag gtagccgaaa actctcgctc aacgccttct
     1261 actctagaac ttttagggaa acactaactc ctaaacaatg tctcaataaa ctatttaatc
     1321 tcgttcctga attaaaagat tactatgacc tttatcagtt actccttttt cacttacaag
     1381 agaagaatac tgagcatttt ttgacttaat taaaaaggtg ttacttctgc tcaatcacac
     1441 ctttaaaacg gctttgaaca ccctgatgcg atacaaaaac tatgtcatta atgccattga
     1501 gcttccttgt tcaaacgcca aactagaagc aaccaataaa ctcatcaaac acatcaaacg
     1561 caatgctttt ggctttcgga actttgataa ctttaaaaaa cgcatttact tcgctttgaa
     1621 cataacaaaa gagaagacga gtctcgtctc ctcacgtgtt tagctataag tcacccacta
     1681 cagttgacaa agagcccaaa aaagaagata tgatcaagca ggcagacaaa actcaccgcc
     1741 atgcttagga cgggcaacac tttcttttct ggcataaatg ctcctttctc aacacacggg
     1801 tcactaaaac ccaaaaaagc cacccctatc agaggtgacc tgaccactaa agggactatc
     1861 ccaattatag cagatcggtg tgggaagtac tagaatcaaa gtaattgatt gtaaagttta
     1921 taatatatac aaattta
//
LOCUS       DAUCWO010000117         1932 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUCWO010000117 DAUCWO010000000
VERSION     DAUCWO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.117"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..460
                     /locus_tag="VGY20_001700"
     CDS             <1..460
                     /locus_tag="VGY20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA helicase"
                     /protein_id="HEQ4274516.1"
                     /translation="ILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACV
                     SSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIA
                     GRVGRSIDRPDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN"
     gene            432..1097
                     /locus_tag="VGY20_001701"
     CDS             432..1097
                     /locus_tag="VGY20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ4274517.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVIYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            1177..1725
                     /gene="raiA"
                     /locus_tag="VGY20_001702"
     CDS             1177..1725
                     /gene="raiA"
                     /locus_tag="VGY20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ4274518.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT          717 a          300 c          349 g          566 t
ORIGIN      
        1 aattttaaaa tacttaaaaa aacaaagaaa aacaggctat ccccttttaa tttttctacc
       61 cactattata atggcagaat ctgttacagc aatcttaaaa gagttattgc ccgccgaaca
      121 aatagcttgt gtttctagtc aatcacaaaa caggaaagaa gatatcacag cttttcgtca
      181 agggaaaaaa acgattttaa ttacaacaac aattttagaa aggggtgtga catttcccca
      241 aattgatgtt tttgttcttg ggtctcatca tcgtgtctat agttcccaaa gtcttgttca
      301 aattgctgga cgagttggaa gatctataga cagaccagac ggaacgttat atttctttca
      361 tgaaggtatc agtaaagcaa tgctactagc aaaaaaagag attaaagaaa tgaattataa
      421 agggtattcg catgatctgt ctactatgtc aacaaattag tcaaacacca ataagtatta
      481 cagaaatcat ctttttaagg cgtatctctt caccgatttg tcaacaatgt caaaaaagct
      541 ttcaaaagat aggaaaaagt gtttgtgcga catgttgtgc aaactcagat ataatagctt
      601 gtcgagattg tctaaaatgg gaaaacaaag gatacaatgt aaatcataga agcttatatt
      661 gttataatgc tgctatgaaa gcatacttca gtcaatataa gtttcaagga gactatttat
      721 taagaaaagt ttttgcagta gaacttgccg atgttatcac caagtactat aaaggctata
      781 tcccagtccc ggttcctgta agtcccggtt gttttcgaga aagacaattt aatcaagtga
      841 gcgctattct tgaggcagct aatgttatct acctttctct ttttgaaaag ctagataata
      901 ctcaccaatc ttccagaaca aaaaaagaga gattattagt agaaaaatct tatcgactac
      961 taaaagtatc aaacattcct gataaaatcc ttatagtaga tgatatttat actactggta
     1021 gtacaattat cgctcttaga aaacaattgg ctaaagtagc aaatagtgac attaaaagtt
     1081 tgtcaattgc acgttaaaac ttgcaaattt tcatgaaagc agtataatag tattaaagat
     1141 aaagtaagcg cttcaagcgc gagaaagagg tatcctatga ttaagttcag tattcgtgga
     1201 gaaaatatcg aagtgacaga agctattcgc gattatgtag aatcaaagct tactaaaatc
     1261 gaaaaatatt ttgctaaaga ccaagaaata gatgcacgtg ttaatttaaa agtttatcgg
     1321 gagagatctt caaaagttga ggtaacgatc ccattagatt cagtaaccct tcgtgctgaa
     1381 gatgtttcac aggatatgta cggctctatt gatttagttg ttgataagat cgaacgccaa
     1441 attagaaaaa ataaaaccaa aattgctaaa aaacatcgcg aaaaggttcc aactggtcaa
     1501 gtctttacaa ctgagtttga agctgaagaa gtcgatgaga taccagaagt acaagttgta
     1561 cgtactaaaa atgtgacatt aaaaccaatg gatgttgaag aagcgcgttt acagatggaa
     1621 ctattgggac atgatttctt tatctacact gattctgaag atggtgcgac aaacatttta
     1681 taccgtcgcg aagatggaaa cctaggatta atagaagcta aatagttcac aactaaaacc
     1741 tacagggatg attatacgta ttttaaaagt gggaaaaagc tattcatgtt tagaggtgac
     1801 atgatgaaaa tgagattaag tgatagatac gacagtcatt atactaacaa aagtatctat
     1861 tacagttcct ctttaagtaa atcttacgta aaattcgttt gaaaaaaaga ttagtcccaa
     1921 ttctgggcta ga
//
LOCUS       DAUCWO010000118         1791 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUCWO010000118 DAUCWO010000000
VERSION     DAUCWO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.118"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..247
                     /locus_tag="VGY20_001703"
     CDS             <1..247
                     /locus_tag="VGY20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ4274519.1"
                     /translation="AIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQ
                     QQKFKQIEDILSDKLAIHVSLLFILAMLMSTIFRYYH"
     gene            341..1564
                     /gene="pepT"
                     /locus_tag="VGY20_001704"
     CDS             341..1564
                     /gene="pepT"
                     /locus_tag="VGY20_001704"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ4274520.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQNVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GRDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1605..>1791
                     /locus_tag="VGY20_001705"
     CDS             1605..>1791
                     /locus_tag="VGY20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274521.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGL"
BASE COUNT          551 a          285 c          377 g          578 t
ORIGIN      
        1 agcgattata ggtgcaactg tattagctat gtacctgact tggacaccag tagggtcctc
       61 aactgtctta ggtgttcaaa gtcgttatct gataggaatt atcccattag tgttgcttct
      121 ttttagtagt caacagcaaa agttcaaaca aatagaggat atcctttctg acaaactggc
      181 tattcatgtt tcgcttttgt ttattctcgc aatgttaatg agtactattt ttagatatta
      241 tcattaaact cttaattaga ggctttaaaa aacttagctt gaataagtat tttaaagcct
      301 tttatgctat aattaaattg agaaattatt gaggtggcct atgaagtacg ataacttgtt
      361 agatcgattt ataaaatatg tcaaagtgaa tacaagaagc gatccagaca gtgagactac
      421 tcctagtacg gagagtcaag aagcatttgc cttaacgatt ttaaagcctg aaatggaggc
      481 aattggtttg caaaatgttc attataatcc agttaatggc tatcttattg gaactctgcc
      541 tgccaataat ccaaccttga cgcgtaaaat tggttttata gcccatatgg atacggctga
      601 tttcaatgct gaaaatgtta atccgcagat tattgacaat tatcaaggaa gggacattac
      661 acttggcagt tctaattata agttggatcc aaaagcattt ccaaatctta ataattatat
      721 tggtcaaaca ttgattacca ctgatgggac tacactttta ggggcagatg ataaatctgg
      781 tattgctgaa atcatgacag caatagaatt cttgacatca caaccacaaa tagagcactg
      841 tgacattaaa gtggcttttg gccctgatga agagattggg gtcggagctg ataagtttga
      901 agtggctgat tttgaggtgg attttgctta tactatggat ggcggtcctt taggcgaatt
      961 gcaatatgaa actttcagcg ctgcagctct agaagtgact tttttaggca gaaatgttca
     1021 tcctggtacg gctaaggatc aaatgattaa tgcccttcag ttagcaatag attttcatga
     1081 gaagctacct gctaaagagc ggcctgaata cacagatggt tatcaaggct tctatcactt
     1141 aaccggacta actggaacag ttgaagaggc acgcgcatct tacattattc gtgattttga
     1201 agaagcatca tttgaagcta gaaaagtgaa agttgaaaat atagctcaat cgatgaatgc
     1261 tcatctagga acaaaacgtg tcttggtaga gcttaatgac caatactaca atatgaaaaa
     1321 agttatcgaa aaagatatga ccgctattga gttagctaaa gaagtaatgg aagagcttgc
     1381 tatcaagcct gtaatcgaac ccatacgtgg aggaacagat ggctctaaaa tttcatttat
     1441 gggtattccc actcctaata tttttgcagg gggtgagaat atgcacgggc gttttgagtt
     1501 tgttagctta caaacgatgg agagagctgt cgatgtcatt attggacttg tttgcaaagc
     1561 ctaattatca tgtaattgat atttagtgga aggggcttat aaaaatgatt aaactatttg
     1621 gtaaaataag gtatcactgg cagccagagt tgtcttggtc tattatttat tggtctattg
     1681 cgtttgcacc tatttttgta ggtttatctt tgttgtatga gcgcactgaa atacctagtc
     1741 gggtttttat tctatttgct atttttgctg tcctagttgg cattggatta c
//
LOCUS       DAUCWO010000119         1709 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUCWO010000119 DAUCWO010000000
VERSION     DAUCWO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.119"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            50..1441
                     /locus_tag="VGY20_001706"
     CDS             50..1441
                     /locus_tag="VGY20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ4274522.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLIFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT          544 a          249 c          287 g          629 t
ORIGIN      
        1 taactatgat acacttagta gaaaatcatt tactatagaa ggtgataaca tgactatttt
       61 tcgtaaaaaa aagaaatatt ccaataaaac agaaatgcaa aggcacttca aagttattga
      121 tttaattttt ttaggcctag gttcaatggt tggaacaggt atctttacag ttactggcat
      181 aggcgctgct aagtatgcag gtcctgcttt aacaatatcg attatcattt ccgcgattgc
      241 tattggaatt ttagccttat tttatgcaga atttgcgtct cgtatgcctt ctaacggtgg
      301 tgcttatagt tatgtttatg caaccttggg agagtttcca gcatggcttg ttggctggta
      361 tattattatg gaatttttaa cagctatatc tagtgttgct gttggctggg gaagttattt
      421 aaaaggatta cttgccaatt ataatatagt attaccaaac gctttaaatg gcacgttcaa
      481 tcttaagaat ggaacttaca tagatattct tcctgtttta gtgatgtttt tcgtgacagg
      541 gattgtccta atgaattcga aattagccct gagattcaat agttttttag ttattttaaa
      601 attttcagca ctggctttat ttatctttgt aggcattttt tttattgatc acaataattg
      661 gtctcatttt gctccatatg gtgtaggaca aattactgga ggtaaaacag gtatatttgc
      721 tggtgcttct gttatgttct ttgcattttt aggatttgaa tctatttcaa tggcagttga
      781 tgaagtgaaa gaaccacaaa aaacaatccc taaaggcatc attttatctt tgattattgt
      841 aacagcactc tatatagttg tcaccactat tttaacggga attgtacact acactaaatt
      901 aaatgttcca gacgcggtgg cttttgcttt aagaaatatt agactttatt gggcagctga
      961 ctatgtgtcg atagttgcta ttttgactct tattacggta tgtatttcta tgacctatgc
     1021 attagctaga acaatttaca gtattagtag agatggttta ttacctaaat cactttatac
     1081 tctcactaaa aaaaataaag tgcctcaaaa tgctactctt gtcactggac tacttgctat
     1141 gatttgtgcg ggaatatttc ctttatcgag tctagcagag tttgttaata tttgtacttt
     1201 agcatattta attattctgt cgggtgcaat tattaaactc agaagaatag aaggagaacc
     1261 aaaagcaaat gaatttaaaa ctcctctagt accattccta ccaatgttgg caattatcat
     1321 ttgtctatca tttatgagcc aatataaagc ttttacttgg attgcttttg ctattgccac
     1381 aattattgga acattaatct acttagctta tggttatact cattcaatag aaaataaata
     1441 atgttttgat aataagattt gacattacat tatagactat aatgtttaga agtaaatgag
     1501 cagtaaaatc aatacctact tacttatcaa agttgtaata tttaaaatta cccaacccct
     1561 agagaattga ttagtctcta ggggttgatt ggtacaatga catagactct aaaacgattt
     1621 taatactaga tatatagata aaaatttcaa aaaagaaaat tagttcattt ttgttataca
     1681 ataaaatttg ttatttagca gtaatataa
//
LOCUS       DAUCWO010000120         1705 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUCWO010000120 DAUCWO010000000
VERSION     DAUCWO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.120"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..229
                     /locus_tag="VGY20_001707"
     CDS             <1..229
                     /locus_tag="VGY20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ4274523.1"
                     /translation="SFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILP
                     RTRIDGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(279..653)
                     /locus_tag="VGY20_001708"
     CDS             complement(279..653)
                     /locus_tag="VGY20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274524.1"
                     /translation="MTQKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEESLGQAKEKVEGIGEATSQ
                     TIQSKMEKLTSDKTDAADDDEK"
     gene            complement(1267..1680)
                     /locus_tag="VGY20_001709"
     CDS             complement(1267..1680)
                     /locus_tag="VGY20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4274525.1"
                     /translation="MKLNAIHHVAIIVSDYNLSKNFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGILVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT          567 a          326 c          232 g          580 t
ORIGIN      
        1 ctctttcttt aaatttttat ggcatattaa tgattttaaa cagggtgatt tttccaaagc
       61 agaggaaatc agtcattttc aagccaatac gcttagctta ctaccacaag ctaaaaaaca
      121 agcgatatta ccacgtacca ggattgatgg ggataaaagt gactatctgc ccattcaatt
      181 agatatcatt ccaaaagcca tttcgattat tgttccaaca gaagattaaa aaagtgccta
      241 atggcacttt ttgtcttatc ttaaaggtat tttaaaacct atttttcatc atcatcagca
      301 gcatctgtct tatcgctggt gagtttttcc attttagatt ggatagtttg actagtagct
      361 tcgccaattc cttctacctt ttctttagcc tgtcccaaag attcttcaac tgtttctgcg
      421 gcaataccac cataaacttc aacagcatct ttggattctt gtactttttc cgatactgca
      481 tccagaactt gcttactttt atcttgcaag taagcgttga gatcatctat ttttttagca
      541 ctatctttca agagttgatc ttttttggaa gacgataaac ttaagtaagc aatgctagct
      601 gctgctgatc caagcaacaa cccccaaaaa acgccatgat ttttttgagt cattgtagac
      661 ctactttctt ttagacggtg ttttactatc gttaaacatt tttttagcaa gttttacagc
      721 aggaaccact aataaaacca attgtaacat ttctgtaagt ttaaaatttc tttttttcat
      781 aatagacttc actccttttt tggtctttaa gcctgagtta atttaacatg attgtttcct
      841 ctaacatcaa caacacatcc tttcaactga agagccacaa aattgtctta tctaaaagga
      901 ccaataaatg gttgtcaaag tgaccacatc acttatttta gtattttcaa tcaaaaattg
      961 aagtaaaatt atctatcgta atagataaaa aaacattaat acgaaaacat ttcaacaaag
     1021 tgacaacaca gttattaact tatattgtca ttataacact gtaagagttt atcgtcaaat
     1081 attttgcttt ttctatagtt attctattaa aaaacttcga caagattact ccaaaaattg
     1141 ctcttcactt tgaaagttaa ccaaatcatt taacgatcaa aagagattaa tcaagcaaat
     1201 gaagatacaa caaataacta tttttaacga gttttatagc acgatccagt cgttaaccta
     1261 ataagtctat tcatgtaatt ctaaaggcaa accatccgga tcaaaaaaga aagtcatttt
     1321 ctcacctgtg taatcatcat gcctgatagg ctccactaga attcctaagc tctttagatc
     1381 atctacataa ctctcaatgt tggtaacacg aaaagcaagg tgcctcaaac cacaggcctc
     1441 acttttaaat tctggttgac ttacacgttt aggaggagct tgatagtagg ggtcacttgt
     1501 caccttacca aaaatctcca attcaatacg accacaagat aaatctaatt tgtaatcatg
     1561 cttgtcagga cgataatttt ctctgataat ttcaaatccg agtttattca cataaaaatt
     1621 ttttgacaga ttataatcag atacaataat agcaacgtgg tgaatggcat ttagtttcat
     1681 aatatttctc ctttcaaaaa aaagt
//
LOCUS       DAUCWO010000121         1627 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUCWO010000121 DAUCWO010000000
VERSION     DAUCWO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.121"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(240..371)
                     /locus_tag="VGY20_001710"
     CDS             complement(240..371)
                     /locus_tag="VGY20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEQ4274526.1"
                     /translation="MTIKNQKKYKGVYCDKNGKIFYQADLGVDPVTGKRVQKKARKN"
     gene            complement(374..595)
                     /locus_tag="VGY20_001711"
     CDS             complement(374..595)
                     /locus_tag="VGY20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEQ4274527.1"
                     /translation="MNIGFLEHTARDIIREAKRIAIKKFEEAREIDQNAVQLSKSPFD
                     NRRLGIAPKDIVEELIGILLSEESLESEN"
     gene            682..825
                     /locus_tag="VGY20_001712"
     CDS             682..825
                     /locus_tag="VGY20_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274528.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            complement(896..1114)
                     /locus_tag="VGY20_001713"
     CDS             complement(896..1114)
                     /locus_tag="VGY20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4274529.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
BASE COUNT          536 a          309 c          219 g          563 t
ORIGIN      
        1 gctgttattc cctttaaaag tttagaacca aaatactgaa taaataactt atgatgtggt
       61 aaggctattt cataagttac agcctgaacc ttttgcttat atgctcttag gtaaatagta
      121 ttcatatagt tttcaaacga tatattgtca ttttcaatac taagattatt actaaaccca
      181 tacctaatcc gaaccagttc atcatacgct tctttcattg tgccaaatgg ctctccatac
      241 taattctttc tggctttctt ttgaactcgt ttacctgtta ctggatctac accaagatca
      301 gcttgataaa atatcttacc attcttatcg caataaacac ctttgtactt tttttgattt
      361 tttatagtca tctttaattc tcactttcta aactttcttc agagagtaga atacctatta
      421 actcttctac aatatcttta ggagcaatac caagtcttct attgtcaaag ggcgatttac
      481 ttaattgtac cgcattttgg tcaatttcgc gagcttcttc aaactttttt atagctattc
      541 tttttgcttc tctaataata tctcttgcag tatgttcgag aaaaccaata ttcatcaagc
      601 tttttatatg ttactatttt cacacctcca ctcctttcaa aaaaattgca acctccgtgg
      661 gtatgactgg ttaaatcact catgggaatc tcacccactg gccttttacg ctggcagcct
      721 gaacgttcag aagtatcatt gtccctattt gtggtcatgg cagatagaga accactctat
      781 tttatagcta ataattatcg ctcctatcac ggcgtatctc actaaattgc tcgacaaaca
      841 agaaagtccc acctcggttt attcagttgt caatgaacaa atcaaggcga gacttttatt
      901 tattatgaaa acgatacgga taatctgttt gccctaataa ataatcaata ctgacgttat
      961 agagtttaga aaactgcaca agaacatctg ccattaaggc aacctctcct ctctcaattt
     1021 ttgcataatt tgcatgagag aaggaaagta aatttgcaac aaacttttga gtgaaatcgt
     1081 tatcttctct caaattccta atacgcggat acatctaaca ttctcctaat atcagtataa
     1141 aggagatttg attaaaaaat gtttaattgt cctatttgga caagactatt aaatataagc
     1201 tagtaatcca ttgaaatttt atagtcattt tttagttaaa caagttagat agtatttata
     1261 acttaaaaaa tcatatttat tgctaggtaa acaaaataga tgtatttaac tacttgtcaa
     1321 acagtcttga ttccaacttt taaatattcg tatatgaagt aaagctactg tgcatgaaac
     1381 aaaaaatata acaataagtg gaacaattgc cattttctgt tggttatata gagtatctga
     1441 tattcttaaa agccatgccc atggaataag aaaccatacc ttatcaccaa ggctcgtttc
     1501 acaaaatgct gcagcaataa cacctaccat tgaaatacct aatgccacac ctgtactaaa
     1561 tatatagcta ataatatgat tgatcagata ggttacatag gcaaaaatga tataaattaa
     1621 actattc
//
LOCUS       DAUCWO010000122         1609 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUCWO010000122 DAUCWO010000000
VERSION     DAUCWO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.122"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1045)
                     /locus_tag="VGY20_001714"
     CDS             complement(<1..1045)
                     /locus_tag="VGY20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ4274530.1"
                     /translation="MQQSMTKVKYLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFI
                     SLLFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFT
                     YFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLK
                     EVNVSQQVKNMLES"
     gene            complement(1026..>1609)
                     /locus_tag="VGY20_001715"
     CDS             complement(1026..>1609)
                     /locus_tag="VGY20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ4274531.1"
                     /translation="AIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHITQMA
                     IALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVDFML
                     IMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAALSF
                     LITMLVWLGLSIMIYLFIMNRFKKGRVNATIYD"
BASE COUNT          619 a          254 c          230 g          506 t
ORIGIN      
        1 gggattctag catattttta acttgctggc taacgtttac ttcttttaga aaatccgcga
       61 catatggggt tttaacctgt ccataaagtc taaaccacat tgcagcaaaa agaaaaatga
      121 gtaatagtcc tcccaatttt ttcaagatga ctaacttggt attttttccg aatttttcat
      181 taggaataaa taatggaagg catactaata aaaagtatgt gaattttgaa agaactagta
      241 acccacatag aactaaataa aggaagaatt tggtattata gttagattta tcttttgatg
      301 agagcaagtt aataaacaat ccaattgtta ctaaaactaa accgatagca aagccgtctt
      361 gattaaaaga tgctgctaaa taaatgttca taggtagtaa agtaacaaca gctatcactt
      421 gtttaaaaga accagacaac ttgatagcta taaaagcaag catagcgtag ctaataaggt
      481 tgcacaatct tccgaaataa tatgttaaaa gtataggtaa atccagcgca tttcctacta
      541 aaatacccaa agcttgtggt atataagaaa aactataata tgcattagtt cctttgatgt
      601 aaggatagct atactctctt gtgctgtgtt tagttgcttt caagtttgat gttataaatg
      661 tttttcctga ctgtttaata agcttatcaa catctttgga tattcttaac tttttgttat
      721 tgttggtaag attaatatca ccttctgaga tataaactgt ccttgaataa tgaacatatt
      781 catcaggaat atcatttata ggtgatatga tagcagatat cgttccaaat actaaaataa
      841 aaatgatcgt agcaacctct aacttttttg gtaaaaacac tatcagtaaa gtgctcacca
      901 caaaaagaag actaataaat tttaaaggta gatttaaact attggcatat aaaataactg
      961 tcagaagtaa tgtcaaaaaa gtcgaataaa tatagttatt attttctttc caaagatatt
     1021 taactttagt catagattgt tgcattaact cttcccttct taaatctatt cataataaat
     1081 agataaatca taatggataa tcccaaccaa accaacattg ttattaaaaa gctcaaagca
     1141 gctcctaaaa tatgatattt cataacaaaa agattggtaa ttaataacga aatcaaaaat
     1201 cctcctgtat atggaataag aagaagttgc tgttttctca tagcggttaa gatattatcg
     1261 ataactgtcg caaaactacc aatcgagcct cctagcataa tcaacataaa atctacccaa
     1321 taatccgtaa ggttagtacc atatagtatt gataaaaagg gaataccaaa caaaccactt
     1381 ccaactaacg ctatcagtga aaaaactcct aaataagcaa acaattgaac ttgtatctta
     1441 ttaaactctt ttatttgacc tctgattaaa gcaatagcca tctgtgtaat atgaggtcta
     1501 aaaaataaaa ttagcaaatt cataacaaag gctggcataa ataaaatatt aaaaattgtt
     1561 tgagagccca aagcaacttc acctaaagtc gtcattagtt caatagcgt
//
LOCUS       DAUCWO010000123         1598 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUCWO010000123 DAUCWO010000000
VERSION     DAUCWO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.123"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            15..983
                     /locus_tag="VGY20_001716"
     CDS             15..983
                     /locus_tag="VGY20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ4274532.1"
                     /translation="MFMMDKTVLYFGLVEISFDVPDFIFQKIKEQNNKIRYYSKSINI
                     LGSVKFINSNQQLIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSDTLLI
                     HAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYA
                     GTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTE
                     ISKIYYIKTDKKEKQIYHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPL
                     INYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            1038..>1598
                     /locus_tag="VGY20_001717"
     CDS             1038..>1598
                     /locus_tag="VGY20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ4274533.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFL"
BASE COUNT          588 a          183 c          291 g          536 t
ORIGIN      
        1 tattcctaag accaatgttt atgatggata aaactgtttt atattttggg ttggttgaaa
       61 tttcttttga tgtgcctgac tttatatttc aaaaaataaa agagcagaat aataaaatta
      121 gatattatag caaatcaata aatatattag gtagtgtaaa atttattaac agcaatcagc
      181 aacttataga atatgatggc aatggaaata ttttagttta tggaagatta aagtatttta
      241 aagaaggaat ttcattatac tacattggag aatacttagc tgaatgtctc ttgattgaac
      301 atagtgacac cttattaatt cacgcagcag cagtttataa tcctttatct ggccattcaa
      361 ttttactatt aggagataaa ggtgctggaa aaactacggt tgcaataagg ctttgtatag
      421 aacatgggta tcaccttatt ggtaatgacc aagtaatatt tggttcgaac tctggaatac
      481 tactaactta tgcagggaca tctttcttta aaataagaag gacagctgtt ttgtcggaca
      541 atttactgtt taaattattt agcaaatttt ttaaccgttc attacaattt aataaggcaa
      601 gttgggatga taaaatttca attttcccaa aggagttggg aatacgaact tgtaattttt
      661 ctactgaaat ctcaaaaatt tattatataa agacagacaa gaaagaaaag caaatttatc
      721 attcaatttg gagtgatagt ttagcaagtc tatatctaaa tgagctattg ggaaggcata
      781 tttctggaca ggttgcatgt tttcaatcag ataatggaaa atatttttct acaatgcctt
      841 tgataaatta tgaaaaaaat aatcgggtac gagaacagat agtgaagggt attttcgcta
      901 aacaaaaatt atttaaaatt acagcagcta cgccagctaa gattgcagaa gcgattatag
      961 aaaatgttaa gaaaaactca tagtgtgtgt gaatttgggg attaatatga agtattgaaa
     1021 aaggagtaag agtaattatg tttgtgatag gcattccaac tttaaatgag gcagataata
     1081 tcagtagatt agttaaacaa attgatgagt atgctgtaaa tttagggaaa gaaattatta
     1141 ttattaattc ggatagtaaa agtacagatg gaactccgca gattttttta gaaaccaaaa
     1201 cttataacac caaagtttcg atagtatcag aagctaaagg aaaaggttat aatgtgagaa
     1261 atattttcga atatgcaata aatcatgtgc cgaatttctc cggtttaatt ttgattgatg
     1321 gagacgttgt ctcaatgaaa aaaatgtggt tagaaaagat gtttatagca attgaatctg
     1381 gaaatgatct tataatccca aattatgcta gaaaatcttt tgaaggaaat gcgacaaacc
     1441 attttattta tcccatgtta gtgaaatttt tcaaacgtga tatgccttat caatgcatct
     1501 ctggtgattt tggattttca agaggattga taaaagattt aactttgaag tgtaattggc
     1561 acaaatatac attaggttac gggatagata ttttttta
//
LOCUS       DAUCWO010000124         1592 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUCWO010000124 DAUCWO010000000
VERSION     DAUCWO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.124"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            4..675
                     /gene="sdaAB"
                     /locus_tag="VGY20_001718"
     CDS             4..675
                     /gene="sdaAB"
                     /locus_tag="VGY20_001718"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ4274534.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            687..1559
                     /gene="sdaAA"
                     /locus_tag="VGY20_001719"
     CDS             687..1559
                     /gene="sdaAA"
                     /locus_tag="VGY20_001719"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ4274535.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
BASE COUNT          515 a          288 c          325 g          464 t
ORIGIN      
        1 cctatgaata cacaaaaatt tcaatcagtt tttgacatca ttgggccagt aatgattggt
       61 ccatcaagta gtcacacagc tggtgccgtc agaattggta aggtagtcca ttccatcttt
      121 ggagatattc ctgatgaagt gacgtttcac ctttataatt catttgctaa aacctacaga
      181 ggacatggta ctgataaggc tcttgttgca ggaattatgg gtatggatac tgataatcct
      241 gatattaaaa attctctaga aattgctcat caaaaaggga taaaaatcta ttgggatatc
      301 ttaaaagata gtaatgctcc ccatcccaat actgttaaaa tcagtgtcaa aaaagctgac
      361 aaaacactta gtgttactgg tgtttcaatt ggaggaggaa acattcaagt aacagaacta
      421 aatggttttt ctgtctcgtt atccatgaat actcctacta tagttactgt acacaaagat
      481 attcccggaa tgatcgcaaa ggtaactgat atcctatctt ccaataatat taatatcgcc
      541 acaatgaatg ttacacgtga atctgctggt gaaaaagcta ctatgattat agaagtcgat
      601 tctagagagt gccaagaagc agccaaccaa attgcaaaaa ttcctcatat ttataatgtc
      661 aacttctttg actagaaagg aaagctatgt tttatactat tgaagaactt gtcaaacaag
      721 ctgaccaaca atttaatgga aatattgctg aactcatgat tgccacggaa gtggagatgt
      781 ctggaagaaa tcgagaagat attatcaaaa tcatgtcacg caatttacaa gttatgaaag
      841 cagctgtaac agaaggacta acctcaacca aatctatcag tggtttgaca gggggcgatg
      901 cggtcaaaat ggataactac attaaaaaag ggaacagcct ttctgatact actattctta
      961 atgctgttag aaatgctata gctgtaaacg aattaaatgc taaaatggga cttgtctgtg
     1021 caacacccac tgcaggaagt gcaggatgct taccagcagt gctcgcaaca gctattgaaa
     1081 aacttgactt atcagaaaaa aaacaattag agtttttatt tactgcagga gcttttggtc
     1141 ttgttattgg aaataacgcc tctatttctg gtgcagaagg tggatgccaa gcagaagttg
     1201 gttctgcagc tgctatgagt gcagctgctc ttgttaaagc agctggtgga acatctcatc
     1261 aagctagtca agctattgcg tttgttatca aaaatttatt aggacttgtc tgtgatcctg
     1321 ttgccggcct agtagaagtg ccttgtgtca aacgcaatgc cctaggagca agtttcgccc
     1381 ttgtcgcagc tgatatggct ttagctgata ttgaatctca aattcctgtt gacgaagtta
     1441 ttgatgctat gtatcaagtc ggctctgcca tgccaactgc tttccgtgaa acagcagaag
     1501 gtggtttagc cgctactcca actggacgac gttacagcgt agaaatattt ggcgaataaa
     1561 tagataggta gaaaaattct tagaaatatt tg
//
LOCUS       DAUCWO010000125         1586 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUCWO010000125 DAUCWO010000000
VERSION     DAUCWO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.125"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(279..1400)
                     /gene="mnmA"
                     /locus_tag="VGY20_001720"
     CDS             complement(279..1400)
                     /gene="mnmA"
                     /locus_tag="VGY20_001720"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ4274536.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVTVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          503 a          332 c          257 g          494 t
ORIGIN      
        1 taatcatgaa ataatctgtt ttacgaaata cctaatcatc aaaatatctg ttgtaatact
       61 agagaaagtg agtctcatga tcgtatgatt tctacagtaa ctaataaagt aatgactcaa
      121 cttttacgaa ctatttttaa aaataatgtg attatattta aaatatacag atatcataaa
      181 ttaatccatt aacgcatgat tggtttttat aaataatatc atatgatgtc aaaggaattg
      241 ttgaatgtcc ctttcttttt atcttaataa aagattcatt aaatgtactg acaaggctgc
      301 ccatttttat aagccatgtc aatcatacca ccaccaaggc attcttttcc gtcatagaaa
      361 acaacagctt gaccaggagt aatcgcgcgc tgtggctctg caaaaacaac ttctgcctta
      421 tctcctcgaa catgcaccgt cacgtgtgaa tcgggttgac gatagcgaaa tttagcagta
      481 cattcaaatg tgaactcttc tggcatctca cgtgtaaaat ggatgactga ggcgtcaaga
      541 ctatttgaca ttagggcttc atggtagaaa ccttgaccaa cgtataaaat attttgcgac
      601 aaatctttac cgacaacaaa ccaaggttga ttatccccac catgctgacc accaatgccc
      661 agacccccac gctgaccaat agtataatac ataaggccgg catgttctcc catatcgcga
      721 ccatcaatag tcatcatgcg acctttttgt gccggtaagt actggctcaa aaactgtttg
      781 aaattctttt caccaataaa acaaatacct gttgaatctt tctttttagc cgttgcaagc
      841 cctgctcgct cagcgatttc tctaacttct gacttttgca aatgaccaag ggggaataaa
      901 gtcttttgta gttgttcctg tgatagttga cttaaaaagt aggtttggtc tttgccatta
      961 tcagctccac gtaacatgtg aaccgtacca ttttcatcac gcttgacttg agcatagtgt
     1021 cccgttgcta cataatctgc acctaaagtc atggcatagt caaggaaagc tttaaatttt
     1081 atttccttat tacacatgac atcgggattt ggagtacgac ctgctcggta ttcggccaag
     1141 aaatattcaa atactctgtc ccaatattct ttttcaaaat tgactgaata gtaaggaata
     1201 ccgatttgat cagcaacggc tgctacatct ttgtaatctt cagtagctgt gcaaacacca
     1261 aattcatctg tatcgtccca gtttttcatg aagacaccaa taacatcata accttgctcc
     1321 tttaaaagta gagctgttac agatgaatca acgccaccac tcataccaac gacaacacgt
     1381 atctttgagt tatctgtcat aaaaaattct cccatcaata ataagataga aaaagataat
     1441 catcatatta tgaatattat gattcttttt cttatcagtt aacgtacgat tgaaggtcgt
     1501 cgttttcaaa agtcgatttg aaggtcgatt ttgaagcgcc atcagcgtaa accttattat
     1561 aacaattact gcgtggaatg ccaatc
//
LOCUS       DAUCWO010000126         1566 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUCWO010000126 DAUCWO010000000
VERSION     DAUCWO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.126"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..975
                     /locus_tag="VGY20_001721"
     CDS             <1..975
                     /locus_tag="VGY20_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4274537.1"
                     /translation="IRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVALTRFVK
                     EISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEK
                     QDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSL
                     ISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEENL
                     IRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYT
                     EDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            1132..1272
                     /locus_tag="VGY20_001722"
     CDS             1132..1272
                     /locus_tag="VGY20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ4274538.1"
                     /translation="MTNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          565 a          197 c          273 g          531 t
ORIGIN      
        1 ataagatata atgagatacc ggagttttct aaccattacc ttagaaatgt ttcatataac
       61 atgttgacat tttatattct gggaatagga acgagtatca gcattgtagt tgctttaaca
      121 cggtttgtaa aagaaatttc tttgaatttc aaggaaatca agaagttagc aaataaaatg
      181 gggatagaag tcctttctga aaatgaaaat tattctaaaa taatcgagtt tgatgatata
      241 cttagaactt tacatattaa aggtgataat ttgaaaagtt tgattgagcg agaaatattg
      301 gaaaagcaag atctatcctt tcaaattgca gcactttcgc atgatataaa aacaccttta
      361 acggttttga aaggaaatct tgagttatta gaattgacta ctttaaataa aaatcaggaa
      421 ggttatattg tatctatgaa caatagtatt tctgtttttg aaggctattt caattcttta
      481 atatcttata cacgcatgct ttcagaggat agatcagtga aactaatttt agttgaggag
      541 ctattaagtg agttacattt tgaagtcgat gacttattga atatcaataa tattgaattt
      601 tctatttgta atagacttat tatcacttct ttttatggag atgaggaaaa tctgataaga
      661 gctctttcta accttttagt taatgcaata agatttatgc ccgtattaga taaaaaaatc
      721 gaagttatac tgtctgagtc aggagagcaa attcattttg aaatatggaa taatggtgag
      781 cgtttcagtg atagtacact gaaaaaggga gataaattat tttacacgga agattatagc
      841 agaggtaaca aacattatgg aatcggtttg gcatttgtta aaggcgttgc tatcaaacac
      901 ggtgggaatc ttcaattgaa taatccagct agaggaggtg ctagcgccat aatctcgata
      961 aaaaagaaaa tttaatagat ttgattgaaa ctggagtatt tcaggcaaaa aatcgtaaat
     1021 tttggtgatt tttggctaaa atactttttt tctgaacaaa ttctgaactt ggggtgtcat
     1081 aataatcatg taaatatatc ggatgcaaaa acgtaagaga ggaactagaa aatgactaac
     1141 acaattaaag actttgatct cgatttgaaa acaaataaaa aagacactgc tacaccttat
     1201 gttggtagcc gttacctatg tacccctggt tcttgttgga aattagtttg ctttacaaca
     1261 actgttaaat aataaagtta tcattatcat caacattata atttcaaata atttgatata
     1321 tttataaatt taggggagta gactgatttt agtttgctcc tttattagta gataaggaag
     1381 ttatacatga ttaaagctca gctaaagaat ctaataaaaa caattcatct cgtaaaagag
     1441 gctggtttct ccctactggt gttctcaatt gctattagct tatttcaagg gattttaccc
     1501 atttttagta tgctactagt tcaaaaaatg ttaaacatta taacgactga tataaaaaat
     1561 tttcac
//
LOCUS       DAUCWO010000127         1565 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUCWO010000127 DAUCWO010000000
VERSION     DAUCWO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.127"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            196..1266
                     /gene="xerS"
                     /locus_tag="VGY20_001723"
     CDS             196..1266
                     /gene="xerS"
                     /locus_tag="VGY20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ4274539.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIISLLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          507 a          247 c          297 g          514 t
ORIGIN      
        1 aaaagaagtg acgttagtat ctgtaaaaaa agagatttgt ctcgtgacat ctttccgaaa
       61 aactgtaatt ttcttgaaaa atatttatag gtgtgatata ctgctcttgt aaagaatgaa
      121 tggcataaaa tacatgagac aatttgcgaa aattcatttt tgaaaaaatt gttattgtta
      181 aggaattatg atgatatgag acgcgaatta ttactagaaa aaattgaaac ctataaggcc
      241 atcatgcctt ggtatgtttt agattattac caatccaagt tagctgttcc atacagcttt
      301 accaccttat atgagtattt aaaagaatac aaacgctttt ttgattggct gatggatgct
      361 gatttaacgc aggcgccaaa gattgctgat attgacttga gcacgcttga gcaccttacc
      421 aagaaggatt tagaggcctt tgtgctttat ttgcgggaac gaccttctct caacacctat
      481 tccaccaaag agggtctttc tcaaaccact attaatcgga ccttgtctgc cttatcaagc
      541 ctttataagt acttgactga agaggttgaa aatgaccaag gagagcctta tttttatcgg
      601 aatgttatga aaaaagtgtc aacaaaaaag aaaaaagaaa ccctggcttc cagagcggaa
      661 aatattaagc agaaactgtt tttaggtgat gaaactctag ctttcttgga ttatgtggat
      721 aaggaatatg agcaaaaatt gtctaatcgt gccaaatctt ctttccgtaa aaataaggag
      781 agggatttgg ctattatttc tctactactt gcttctggtg ttcgtttgtc tgaagcagtg
      841 aatcttgatt taaaagatgt caatctaaat atgatgatta tcgaagttat tcgcaaaggc
      901 ggaaaacgtg attcagtcaa tgtagcaggt tttgcgaaag gttatttgga gtcttattta
      961 gctgttcgtc agagacgtta caaggctgaa aaacaggatc ttgctttctt tttaacagaa
     1021 taccgtggcg ttccaaatcg aatggatgct tctagtatag aaaagatggt tggcaagtac
     1081 tctgaggatt ttaaaattcg tgtgactcct cacaaattgc gtcatacgct agcgacaaga
     1141 ctatatgatg ctactaaatc tcaggtgtta gttagtcatc aattaggcca ttcctctact
     1201 caagtcacgg atctttatac ccatattgtc aatgatgagc aaaaaaatgc tttagataac
     1261 ctttaatgtt acataattgt ttttatgtaa cattaaaggt aaaaatagct tcaaaattaa
     1321 ctttaataga tggctactat taaccgtgac agtttggtgt cagaccgttt tccaaactac
     1381 tcaagccact tagaaaaagg aatatcactg ctaactttgt cccttctttg aatgactttt
     1441 tttgcgatgt ttatagatta aatctagtag ggttctcatc aaaaagatga gataagccta
     1501 tttttagtat gtcagtactt ttatttagta tgataatatg ttatcttgta aagtagcttt
     1561 tagga
//
LOCUS       DAUCWO010000128         1539 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUCWO010000128 DAUCWO010000000
VERSION     DAUCWO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.128"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            17..937
                     /locus_tag="VGY20_001724"
     CDS             17..937
                     /locus_tag="VGY20_001724"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ4274540.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNKKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            991..1239
                     /locus_tag="VGY20_001725"
     CDS             991..1239
                     /locus_tag="VGY20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ4274541.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1364..>1539)
                     /locus_tag="VGY20_001726"
     CDS             complement(1364..>1539)
                     /locus_tag="VGY20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4274542.1"
                     /translation="EKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNITVK
                     DNDPKGSIFEVKL"
BASE COUNT          541 a          288 c          222 g          488 t
ORIGIN      
        1 atgattagga gctaccatgt caaatgaatt tatcaacttt gaaaaaattt ctagagagtc
       61 ttggaaaaca ttacatcaaa aagcaaaagc tcttctcaca caagaggaac ttaaaagtat
      121 tactagctta aatgataaca tcagtattaa cgatgtcatt gatatctacc ttccattaat
      181 taatctgatc caggtttaca aaattgcgca agaaaatctt tctttttcta aaagcctttt
      241 tctaaaaaaa gacattcaat taaggccttt tattattgga atttctggtt cagtagctgt
      301 tggtaaatca acaactagtc gtcttttaca acttttatta tcgcgaacgc accccaatag
      361 tcaagtcgaa ctggtaacga ctgatggttt tttatatcct aatcagtttc ttattgaaca
      421 aggactgtta aatcgtaaag gatttccaga atcttataac atggaacttc tcttagattt
      481 tctagattcc atcaaaaatg gtcaaactgc ttttgcaccc gtctattctc atgatattta
      541 cgatattatc ccaaataaaa aacaatcatt taataaccct gacttcctta tcgtcgaagg
      601 tattaatgtt tttcaaaatc aacaaaacaa tcgtttatat atgagtgatt actttgattt
      661 ttctatttat attgatgcag acagcagtca catcgaaacc tggtacattg aacggtttct
      721 aagcatttta aaattagcaa aacgtgaccc tcataattat tatgcccaat atgcacaatt
      781 acctcgatct gaggctatag catttgcgcg caacgtttgg aaaaccgtta atttagaaaa
      841 tcttgaaaaa ttcattgaac ctacacgtaa ccgtgctgaa ttaattttac ataaaagtgc
      901 tgatcataaa attgatgaaa tttacctcaa aaagtgataa agggttgcca aacgcatata
      961 ttttctatat aataatgtag ttagattaat ttggaggtga aaacattggc aaatattaaa
     1021 tcagctatta aacgcgctga acttaacgtt aaagcaaacg aaaaaaactc agcacaaaaa
     1081 tcagctatgc gtactgctat caaagcattt gaagctaacc catctgaaga gcttttccgc
     1141 gctgcttctt caagcatcga taaagctgaa tcaaaaggtt tgattcacaa aaacaaagca
     1201 agccgcgata aagcacgcct tgctgcaaaa cttggctaat aaatatccaa tgaaaactcc
     1261 aactggagtt tttttatacg gtaaaaactc aaaaaataca ataaagcgtt gacagttaaa
     1321 aataacttta aaagggctga ccttattggt cgcccttttt agcttatagt ttgacttcaa
     1381 aaatactacc tttaggatca ttatccttta ctgtaatatt tccttttaaa gacatcacga
     1441 tttgctgagc taaagccaac cccaagccaa atccacctgt ttgccgtgtt ctagctttgt
     1501 caactcgata aaaacgatca aaaatctttt tcttttctt
//
LOCUS       DAUCWO010000129         1500 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUCWO010000129 DAUCWO010000000
VERSION     DAUCWO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.129"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..781
                     /locus_tag="VGY20_001727"
     CDS             <1..781
                     /locus_tag="VGY20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4274543.1"
                     /translation="HFMNQKLKNYSSGMQVRLAFSVAIKAQGDVLILDEVLAVGDEAF
                     QRKCNDYFMERKDSGKTTILVTHDMGAVKKYCNRAVLIEDGLVKAYGEPFDVANQYSV
                     DNTETAEDAMNAEKISVSDIAKDLKVSLISNPRITPNDTITFEVSYEVLKDDFTYIAF
                     SLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQCQLSQLNDIKLKLEVTVRDKNGQMLL
                     FSTAEQSPQIVIQRNDIDQDDLSALDSASGLYQRNGKWVFH"
     gene            806..>1500
                     /locus_tag="VGY20_001728"
     CDS             806..>1500
                     /locus_tag="VGY20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ4274544.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTIYRMTTDSDSR
                     PTDTPKMIHRIQKL"
BASE COUNT          529 a          227 c          277 g          467 t
ORIGIN      
        1 tcattttatg aatcaaaaat taaaaaatta ttcttctggc atgcaggtac gtttagcatt
       61 ttcagtggct attaaagccc aaggtgacgt tcttatttta gatgaggtgc ttgctgtagg
      121 agatgaagct tttcagcgaa aatgtaatga ttattttatg gaaagaaaag acagtggtaa
      181 gacgactatt ttagtgacac atgatatggg agctgttaag aagtattgta accgtgcagt
      241 tcttattgaa gatggtcttg ttaaagcata tggagaacca tttgatgttg ctaatcagta
      301 tagtgtagat aatacagaga cagctgagga tgcaatgaat gctgaaaaaa tatctgtatc
      361 tgatattgcc aaagatttga aagtgtcatt aatctcaaat ccaagaataa caccaaatga
      421 cacaatcaca tttgaagttt cttatgaagt tttaaaagat gattttacct acattgcctt
      481 ttctttaacg gatattgata gaaacatttg ggtttacaac gacaattctt tagattatcc
      541 aacacaaggt ataggtagaa aatgcatttc ctaccaatgt caactatctc aacttaatga
      601 tataaaacta aaattagaag taactgtccg agataagaac ggtcagatgc tactcttttc
      661 aacagcagaa caatcacccc agatagtgat tcaaagaaac gatattgatc aagatgatct
      721 gtcagctcta gattcagcaa gtggcttgta tcaacgtaat gggaagtggg tttttcatta
      781 atgagtaata gctagaaggg aatttatgta taaagtttca attatttgca ccaattataa
      841 caaagctcct tggatatctg atgcacttga cagtttttta agccaagtga cagattttga
      901 ggttgaaatt attgttattg atgatgcttc tacagatgac tcacgagaga ttttaaagag
      961 ttatcaaaaa aagtcttcag gaaaaattaa attactcttt aacgaaacta atattggaat
     1021 aacaaagact tggataaaag catgccttta tgcaaaaggc aaatatattg ctagatgtga
     1081 tggtgatgat tactggacgg atagttttaa actccaaaaa caagttgatg tcttagaagc
     1141 ttccaaacgg tcacgatggt gtaatactga ttttgacttt gtaaatagtc aaggcgactt
     1201 attatatgct aatgcttttt catctggtca tacacctttg acagatactt acgaaaaagt
     1261 tttagcctta aaagggatga cgatggcatc gacatggtta gttgatgctg atttaatgag
     1321 acaagtaaac caaaaaatca atgtagatac accagacgat acttttgata ttcaactaga
     1381 gttatttcaa ctgactcaac tgacctatat taaagactca acgacgattt atcgcatgac
     1441 aaccgattct gattcaagac ctactgatac accaaaaatg attcatcgta ttcaaaaact
//
LOCUS       DAUCWO010000130         1400 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUCWO010000130 DAUCWO010000000
VERSION     DAUCWO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.130"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            311..>1400
                     /locus_tag="VGY20_001729"
     CDS             311..>1400
                     /locus_tag="VGY20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ4274545.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSEDYNK"
BASE COUNT          528 a          199 c          210 g          463 t
ORIGIN      
        1 ccaaaaaaag gaaaaatcag gataatttat ttaatataaa ttaaataaat tatctcaaaa
       61 taattgaatt gttataaaat attttagcaa acttttcaaa tagttttatc taattttgtg
      121 aaaaaaatag atatattttg atttttaaaa aatatcaaga aatatgataa ctggtaaagg
      181 atatattgta gaaaactata ttttactaac actagaatcc cgacaattaa gtcaaaaaaa
      241 gttatcactc acctaacctg aataaaaatg gtataatcgt aaggatcaca ctatttatta
      301 aggaaacatt atgaaaattg ttcagggagt acaacttcat ctgattaaga ccaaacagtt
      361 taaaaccaat catattacct ttcgtttttc aggtgatttg aaccaaaaaa cagtagcaaa
      421 aagagtctta gtggcacaga tgttagcaac agctaatgag tgttatccaa ctgttaggca
      481 gtttagagaa aaattagcac gactttatgg agctagcttg tcaactaacg tgttaacaaa
      541 agggttagtt catattgtgg atatcgatat tacttttatt caagatagat atgcttgcaa
      601 tggggaaaaa atactagatg aaatgattca gtttttaaaa gatatcttgt tctcacccct
      661 attatccatc gcccagtatc agcctaaagt ttttgagact gaaaaaaata atttgattaa
      721 ctacatcgag tcagatagag aggattcttt ttactacagc tctcttaagg taaaagaatt
      781 gttttattgt aataagaatc tacaaatgtc agaatatggt agcccagaac taattgcaaa
      841 agagactgcc tatactagct atcaagaatt tcataaaatg ctcaatgaag atcagattga
      901 tattttcatt ttaggagatt ttgatgatta tcgcgttgtt cagctaattc atcaatttcc
      961 acttgataac cgaaataaaa acctaaattt ctttcattta caaaattctg tcaatattat
     1021 taaagaatct attgaaaaaa gagcagttca tcaatctatt ttacaattag cttatcactt
     1081 cccttcagtt tttggacaac gtgactatta tgctttagtt cttttaaatg ggttgttagg
     1141 ctcttttgca cattcccgtc tctttataaa aattagagaa gaagaaggac ttgcttatag
     1201 tatagggtgt cgttttgata gttatacagg tttatttgaa atttatactg gaattgacag
     1261 tcaacatcgc actaaaacgt tacaactaat catccaagaa ttaaatgcca ttaaaatggg
     1321 acgattctca gaacaattga ttaaaaagac cagatcgatg ttgttgaata acgccttatt
     1381 atcagaagat tataataaaa
//
LOCUS       DAUCWO010000131         1342 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUCWO010000131 DAUCWO010000000
VERSION     DAUCWO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.131"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..306
                     /locus_tag="VGY20_001730"
     CDS             <1..306
                     /locus_tag="VGY20_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEQ4274546.1"
                     /translation="AKMILNSSAKTIGIYRINSKKDSDNCRESSTIDVAKLLKSSGKD
                     VIIFEPLINQKKFLGCPLINDFNEFIKYSDIIVANRIDDALRKCNSKVFTRDIFQYD"
     gene            391..>1342
                     /locus_tag="VGY20_001731"
     CDS             391..>1342
                     /locus_tag="VGY20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4274547.1"
                     /translation="MNNRKKNIQYLVYSKVIYRIGDVMFDFANNTFLAGLNPASLSLV
                     AVYQFLESVIGVLFNLFGGVIADSFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAI
                     VLTNVILAFMSAFSSPSYKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYK
                     LLGIHGVLLLDGLSFLIAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHK
                     SIFIITVLSALVNFFLAAYNLLLPYSNQMFGEISAGLYGTFLTAEAIGGFIGAILSGF
                     VNKELSSMRLILFLSLSGLMLMLAPPFYIMFHNAIILALSP"
BASE COUNT          449 a          172 c          221 g          500 t
ORIGIN      
        1 gcaaaaatga ttttgaacag ctcagctaaa acaataggaa tttatagaat taattccaaa
       61 aaagattcgg ataattgtag ggaatcttct acaattgatg ttgctaaact tttaaaaagc
      121 agtggtaagg atgttatcat ctttgagcca ttaattaacc aaaaaaagtt tttggggtgc
      181 cctttaatta atgattttaa tgaatttatt aaatattcgg atattatagt tgccaataga
      241 atagatgatg ctctgagaaa atgtaattca aaagttttta cacgtgatat ttttcagtat
      301 gattaacaaa aacatttttg cgaaaaaata tttttaagta ataaattcag ttaaaagtta
      361 ttatatctga aaattttaaa ggtctttaaa atgaacaata ggaaaaaaaa tattcaatat
      421 ttagtgtata gtaaagttat atatcgaatt ggcgatgtta tgtttgattt tgctaacaat
      481 acttttcttg ctgggcttaa tccagcgtct ctatcattag ttgcagtata tcagtttcta
      541 gaaagtgtca taggtgttct atttaattta tttggtggag tcattgcaga cagtttcaag
      601 cggaaaaaaa ttattattac tacaaatatc ttatgtggca cagcttgctt agtactctcg
      661 ttcctaacaa aagagcaatg gttggtttat gccattgtac taactaatgt tatcttggca
      721 tttatgagtg ctttttctag tccatcctat aaagcattta caaaagaaat tgttaaaaaa
      781 gatagtatat cacaacttaa ttcattgcta gagacaacaa gtactgtaat caaagtaaca
      841 gttcctatgg tagcaatttt cttatataag ctacttggta tacacggtgt attactattg
      901 gatggactat catttctaat agctgcttta ctaatttcct ttattttacc tgttaatgac
      961 gaagtggtga taaaggagaa ggtgacaatc agagaaatat ttaacgattt aaaaatagga
     1021 tttaaatatg tttacagcca taagtccatc tttattatta ccgttctttc tgcacttgtt
     1081 aatttttttc tagccgctta taatttattg ttgccctata gtaatcaaat gtttggagaa
     1141 atttcagctg gactttatgg cactttttta acagcagaag caattggtgg atttattggg
     1201 gcaatattaa gtggttttgt gaataaagaa ttatcaagta tgagattgat attgtttcta
     1261 tctttatcag gactgatgtt aatgttagct cccccatttt atattatgtt tcataatgca
     1321 attattttag ctttgtcacc ag
//
LOCUS       DAUCWO010000132         1322 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUCWO010000132 DAUCWO010000000
VERSION     DAUCWO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.132"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            281..562
                     /locus_tag="VGY20_001732"
     CDS             281..562
                     /locus_tag="VGY20_001732"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4274548.1"
                     /translation="MTVLNYVNTGDAKSIHNHLFHYTRQVRREVKVITIGILSAHYYE
                     ITMLFLQAKIILNCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(1037..1124)
                     /locus_tag="VGY20_001733"
     tRNA            complement(1037..1124)
                     /locus_tag="VGY20_001733"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Ser,seq:gct)
     gene            complement(1134..1207)
                     /locus_tag="VGY20_001734"
     tRNA            complement(1134..1207)
                     /locus_tag="VGY20_001734"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Ile,seq:gat)
     gene            complement(1242..1312)
                     /locus_tag="VGY20_001735"
     tRNA            complement(1242..1312)
                     /locus_tag="VGY20_001735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1278..1280),aa:Gly,seq:tcc)
BASE COUNT          459 a          284 c          175 g          404 t
ORIGIN      
        1 gtttttttat aagctaacta tcgaaaactg gtcatctata aatcttagta ttccatcact
       61 caatcacaat tcataaagac tcgcaaactt tcacaacttt taattctata ctttctgtag
      121 taggtaaatc cacctcaaag attatagagt ctttttaaac atatgtaaaa agttccatat
      181 gacgatctat aatgaaaagc gaccaaacta tcatttcaga aagactcata tagaataatc
      241 cgattatagt ataatttcat taggaatcta aaaccttagc atcacggttt taaattatgt
      301 aaatactggg gacgcaaaaa gcattcataa tcatctcttt cattatacca gacaggttag
      361 aagggaggtc aaagttatta ccataggcat actcagtgcc cactactatg agattactat
      421 gctttttcta caggctaaaa ttatcctcaa ttgttttcat atcccccaac gtcttagaca
      481 agctataact cgctttcgta tccgaatcat aaatacttct gctataaaac ctatcgcgta
      541 tagaactctc aaactttact gaaaactcat acagcatgat tcacggaagc tcatcaataa
      601 tagctttatt gcccaatttt tcgtacgccc ataactaaca tctagaaaag aaaagctctt
      661 actcacaaga tctctgaatt tcaataatta ttcctaactc tatcaactct tgctcttccc
      721 atttttcaga caatttttaa aatttttcta aaagaaaaga acaagattag caatatactt
      781 aaactcaact actctaaggc caaattagaa acagtcaaca accttatcaa agccatcaag
      841 ttaaacgttt tacttctcta cagtagtcaa agagcctatc attttagtta tcgaaataat
      901 gaacgattta aatattctat ttagttggtt ttgaagatta caaaaaaaag acgataaccg
      961 tcttctttcc cctttattta taaatcatcc attaatatgt ttcatttaac atattaacca
     1021 aaatatcttt acaaaacgga gaataaggga ttcgaaccct tgcgccagtt acccgaccta
     1081 acgatttagc aaaccgtcct cttcagcctc ttgagtaatt ctccaaatat atatgggcac
     1141 gagtggactc gaaccaccga cctcacgctt atcaggcgtg cgctctaacc acctgagcta
     1201 cgcgcccaag cttttattga tatagcttgg gaaaactata aagcgggtga cgagaatcga
     1261 actcgcgaca acagcttgga aggctgtagt tttaccacta aactacaccc gcaatatgat
     1321 aa
//
LOCUS       DAUCWO010000133         1198 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUCWO010000133 DAUCWO010000000
VERSION     DAUCWO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.133"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            31..804
                     /locus_tag="VGY20_001736"
     CDS             31..804
                     /locus_tag="VGY20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4274549.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
BASE COUNT          411 a          185 c          224 g          378 t
ORIGIN      
        1 tataccctaa gacactgata ggagataaga atgaaaaaga aagagcgtca tgaaaaaatt
       61 ctcgatatct taaaagttga cggttttata aaagtaaaag atattatcga tgaaatgaac
      121 atctcagata tgaccgctag acgagatctc gatacattgg cagataaagg attacttatc
      181 aggacccatg gtggtgctca atatttggat tattcttcag caaaggacga aggtcacgag
      241 aaaacacaca ctgaaaagaa ggtcttacag actaccgaaa aaaaacaaat tgcacaaaga
      301 gctaaaaaca ttattaaaga tggcgaaact gtctttatag gtccagggac aacattagag
      361 catctagctg tcgaattgaa aaatagaaat atcagggtca ttacaaatag ccttcctgtt
      421 tttttaattt tgcacccaag cactactatt gatttgattt taattggtgg tgaatgtcgt
      481 gaaattactg gagcttttgt tggtgctatt gccattaata atctagaatc attaactttt
      541 tcaaaggctt ttgttagcgc aaatgcagtt tacaataatt ctattgcgac gtacagtgat
      601 ctcgaaggag aagttcaaag ggttgcattg aataatgctg ttgagaaaat ccttctagtt
      661 gatagtacca agtttgaaag ttatgacttc tataatttct accaactaga tcaagtggac
      721 ttaattgtga ctgataaaaa tgtgacaact gatatcatag aaaagtacaa gaaattctca
      781 aaaatcataa tcgctgacaa gtaaaggact tactcatatt aaaaagcact tcagggttta
      841 gatatatccc ctgtcaagta aaaagggagc atatctgaaa gtagtgtttg tattataaag
      901 aagtttcttg ctaacttatt ttctggtttt tttcagactt atcagtcttg tactgttcct
      961 ttttctgatg aataacgtgg aaataggcag acagctatat atttcacctc tagaatggtt
     1021 gtcattactg tcatcaagac atgcttgttc tttatcgtat ggagtgaagc tttcttttag
     1081 acatcaacat acctcttaag tagattttgg ttcgttacca tgtctactta agaggtgtct
     1141 tcaaagcgct ttaaaacgtt gatataacag tatattaacg ttttgagaat tgtgaagc
//
LOCUS       DAUCWO010000134         1042 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUCWO010000134 DAUCWO010000000
VERSION     DAUCWO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.134"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            945..>1042
                     /locus_tag="VGY20_001737"
     CDS             945..>1042
                     /locus_tag="VGY20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4274550.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDN"
BASE COUNT          368 a          114 c          183 g          377 t
ORIGIN      
        1 tgaaagtcat tcaagacagt ttattaagtc cagtgatatc ctatccagca atgatatcga
       61 gaatttaaag ttaagagcgt tgtttttatt acaggaagaa aaacagtatc cacatgggct
      121 gtcagaggta gaatgggacg gaattttgtc taatacaatt aacaatattt atagatatag
      181 tagtgaggaa cattcgttat atttattacc actttattta ttgagagtat acaattatct
      241 aaagtacaac taagttttca atttttatta atcttgagaa gaagtgtgat tggtggaata
      301 tatttcaata tgtttagctt atctaaataa taaatacagt atgtaagtaa atttatttat
      361 aatatttttg atactttgag tagtaacgat aatatttaat tttttgatgg attactttaa
      421 ttgactttag tgtgttagat gccagtttgg atttttgata tttgtgttga gtttaaaaga
      481 taaaaacact taatgaaaat ctgttttttc ttagaaaggg gaccagtatg attgagacgc
      541 taatgagtac gttggaaatg gttagaaaaa aatcctagta ggactgcaat cgaagatgac
      601 tttgaggaat ataaaaagag aaatctacca atctttgtca gtgaagatga tagtgtgatc
      661 tattatagac cagggttcat ttttaatgaa ttctatccca atatttcagg ttggagacaa
      721 gaactaaaga ctaagcttac tagtatttct ttaatttctt tacattttat tggtgttgtt
      781 gacgaacaaa aaaaggggga atatctaggt tctttttctg atacatctga attcttaagt
      841 agacatccaa aaatttatat catctcattt aggtataaaa atcaatttta tattctttct
      901 aacaaaaaaa ttgatttata aaaagtttta gaatagaggt taacatgaaa attacagttg
      961 taggcattgg atacgttgga ttatcgatag gactcctact tgcaaaggaa cacgacgtta
     1021 ccttttttga tattgataat aa
//
LOCUS       DAUCWO010000135         1012 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUCWO010000135 DAUCWO010000000
VERSION     DAUCWO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.135"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>1012)
                     /locus_tag="VGY20_001738"
     CDS             complement(<1..>1012)
                     /locus_tag="VGY20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4274551.1"
                     /translation="RGGPSKMGRKRKKNLEEMTEVERLQKELEYPRAENAVLKKLREY
                     RLRRRSKTQRATEIIQALRNQFPLEMLLEILDLSRSTYYYQVKRLAQGDKDIELKHVI
                     REIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAIRIRRKRKYSSYKGEV
                     GKKADNLIKRHFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPN
                     LKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNG
                     MMESFFGILKSEMFYGLETTYQSLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTK
                     SF"
BASE COUNT          264 a          214 c          195 g          339 t
ORIGIN      
        1 tgaaaggatt tagttctgta ttggacagga ctaagtcctt ttagttttgc tttaattcgt
       61 ttgttgttgt agtaaaaaat gtaatctgta atagcttctt caagcttgtc aagtgattga
      121 taagttgtct cgagtccata aaacatttca gatttgagaa tgccaaagaa ggattccatc
      181 atgccgttat ccgggctatt ccctttgcgg gacgtggatg gcaaaatacc tttagactct
      241 aaaaaatcat ggtaggactg gtgttggtat tgccacccct ggtcactatg aagaatagtt
      301 ccgctgtatg aatccgctgg aaaagtcttc tcaagcatgg tttgaacctg tttcaagtta
      361 ggagaccgag acagagtgaa atcaataatc tcactgttat agccgtcaag aacaggtgat
      421 aagtagagtt ttccttcagg taaggcaaac tccgtcacat cagtgtaaca tttttcgtaa
      481 ggtttagagc cttcgaagtg acgtttaatc agattatcag ccttcttacc aacctctcct
      541 ttgtaagaag aatacttgcg cttccgacga atgcggattg ctaggcccat gactttcatg
      601 agacgttgaa ctttcttatg attgaccaca aatcctcggt tacgcaactc catatgaata
      661 cgacgataac cgtaattacc tttatgctca tcataaatct ctcgaatcac atgctttagt
      721 tctatatcct tatctccttg agctagtcgc ttcacttgat agtaataggt ggagcgtgat
      781 aaatcaagga tttcaagtag catctctaag ggaaattgat tccttaatgc ttgaatgatt
      841 tctgttgctc tttgagtttt gcttcgtctt ctcaagcggt attctctcag cttttttagc
      901 acagcattct ccgctctagg gtattctaat tccttctgaa gacgttcaac ctctgtcatc
      961 tcttccaaat ttttcttacg tttacgcccc atcttactcg gtccccctct tg
//
LOCUS       DAUCWO010000136          921 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUCWO010000136 DAUCWO010000000
VERSION     DAUCWO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.136"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..741
                     /locus_tag="VGY20_001739"
     rRNA            <1..741
                     /locus_tag="VGY20_001739"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            827..>921
                     /gene="rrf"
                     /locus_tag="VGY20_001740"
     rRNA            827..>921
                     /gene="rrf"
                     /locus_tag="VGY20_001740"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          239 a          197 c          266 g          219 t
ORIGIN      
        1 gcgttgttgg gatactaccc ttgtgttatg gctactctaa cccagatagg ttatccctat
       61 cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaag agtaacggag
      121 gcgcccaaag gttccctcag attggttgga aatcaatcgc agagtgtaaa ggtataaggg
      181 agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag tgatccggtg
      241 gtaccgaatg gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta
      301 tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat
      361 cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc
      421 tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta ggaaatttga
      481 gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt accagttgtt
      541 ttgccaaagg catcgctggg tagctatgta gggaagggat aagcgctgaa agcatctaag
      601 tgcgaagccc ccctcaagat gagatttccc atgattttat atcagtaaga gccctgagag
      661 atgatcaggt agataggtta ggagtgtaag tgtagcgata catgtagcgg actaatacta
      721 atagctcgag gacttatcca aaaagaaata ttgacaacgt tacggattct tgttagacta
      781 tagatattca attttgagtg ggttatgaaa atagcacatt tacaagttaa gtgacgatag
      841 cctaggagat acacctgtac ccatgccgaa cacagcagtt aagccctaga acgcctgatg
      901 tagttggggg ttgccccctg t
//
LOCUS       DAUCWO010000137          918 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUCWO010000137 DAUCWO010000000
VERSION     DAUCWO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.137"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            86..358
                     /gene="rpsP"
                     /locus_tag="VGY20_001741"
     CDS             86..358
                     /gene="rpsP"
                     /locus_tag="VGY20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ4274552.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            368..607
                     /locus_tag="VGY20_001742"
     CDS             368..607
                     /locus_tag="VGY20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ4274553.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT          296 a          150 c          162 g          310 t
ORIGIN      
        1 ttaaactgag ttatactgtt taacatagga atttagcgta gctgtatttc ttatacatta
       61 ctaaaagaaa agagactatt taaaaatggc agtaaaaatc cgtttaactc gtatgggttc
      121 taagaaaaaa cctttctacc gtattaatgt tgcagattca cgtgcaccac gtgatggtcg
      181 ttttatcgaa acagttggaa catacaaccc attagtggct gaaaatcaaa ttactattaa
      241 agaggatcgt gttcttgagt ggttatctaa aggagcgcag ccttctgata ccgttcgtaa
      301 catcttgtct aaagctggtg ttatggctaa gttccacgac caaaaattct ctaaataata
      361 atagcctatg gataccattg aaaatcttat tatcgctatt gtgaaacctt tgatttcaca
      421 accagataat cttaccatta aaattgaaga tactcctgac tttttagagt atcatcttga
      481 cttggatgct caagacatcg gtcgtgttat cggcaaaaaa ggtcgaacta ttactgcgat
      541 aagatcgatt gtctactcgg ttccaacact aggaaaaaaa gttcgcttag ttattgatga
      601 gaaataatca ctaacttgag atggattgaa aagcttgttg actttaagtc aacaagcttt
      661 ttgaatagca aaaatcttaa tatttctgaa atgaaaagat tagatttttc tgtctttaac
      721 taaaaagaag ttaaagaatg gcttagctga ttgcctttat aagagattat actggttttt
      781 gatagctgct gtcatgtaaa atagctacaa ttcatcttac gagattgacc ttagcattgc
      841 cagtgcagtc tttattttcc aacctgctgt ttgttgtatg tttatagttt cctaaaaaat
      901 gctctctcag ttttttgg
//
LOCUS       DAUCWO010000138          855 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUCWO010000138 DAUCWO010000000
VERSION     DAUCWO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.138"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>855)
                     /locus_tag="VGY20_001743"
     CDS             complement(<1..>855)
                     /locus_tag="VGY20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEQ4274554.1"
                     /translation="LVLKSVEAFIMGLEIYNKPTIKYRIEGFSFFICNAWELMLKAEM
                     LNRGFSIYYSDNPERTLSLEYVLKKIYSDENTRIRLNLEKIIELRNISTHFITEDYEI
                     KYAPLFQACVLNLASEMKRFHNKDITESIAHNFLTISESYEPLSNEEIKLKYPSEIAE
                     KFIKQANEVDVLSKEYNSEKFSIDIRQNLYITKKKDEADFVVSISKDAETPVSLVKEL
                     KDPSDTHKYSYSNVITAVQERLKKSAIKLGYEKGFNTYVLNLVIDFYNIKSETKFSYC
                     HLIGSTQTY"
BASE COUNT          271 a          132 c          109 g          343 t
ORIGIN      
        1 ataagtctgt gttgatccta taagatgaca atatgaaaat tttgtttcac tcttaatatt
       61 atagaaatca atcactaaat ttaatacata agtattgaac cctttttcat agccaagttt
      121 tatcgcagac ttttttagcc tttcctgaac tgctgttatt acattgctat aggaatattt
      181 atgagtatct gacggatctt ttaattcttt aactagtgaa acaggggttt cagcatcttt
      241 tgaaatacta acaacgaagt cagcctcatc ttttttctta gtaatataaa gattttgtct
      301 aatatcaatt gaaaattttt cagaattata ctctttactc agtacatcaa cttcattagc
      361 ttgtttgatg aatttttctg caatttctga aggatatttt agctttattt cctcattgga
      421 taaaggttcg taactttctg atattgttaa aaaattatgt gctattgatt cggtaatatc
      481 tttattatgg aatcttttca tttctgacgc taaatttaaa acgcatgctt gaaataatgg
      541 agcatattta atttcatagt cttcagtaat aaagtgggtg ctaatatttc ttaattcaat
      601 tattttttca agatttaacc gaattctggt attttcatca gaatatatct tttttaaaac
      661 atattctagg ctcaatgttc tttctggatt atcactgtaa taaatactaa accctcggtt
      721 aagcatctca gcttttagca ttagttccca agcattacat ataaaaaaac taaagccttc
      781 aattcgatat tttattgtcg gtttattata tatttcgagc cccataataa aagcttcgac
      841 actttttaaa actaa
//
LOCUS       DAUCWO010000139          822 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUCWO010000139 DAUCWO010000000
VERSION     DAUCWO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.139"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            310..558
                     /locus_tag="VGY20_001744"
     CDS             310..558
                     /locus_tag="VGY20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ4274555.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            595..>822
                     /locus_tag="VGY20_001745"
     CDS             595..>822
                     /locus_tag="VGY20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ4274556.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFV"
BASE COUNT          245 a          112 c          156 g          309 t
ORIGIN      
        1 tttctgtatc atataaaagt taaaaaattt taagttttag gagcatatca gttgcatttt
       61 aaatttaaag gtgtataata acaatataaa ttaaataacg agtggtcact ttataaaata
      121 ttatcaaaat aacattttat aggattttga aatatcggta ttcaaacaaa aaataaacta
      181 acactttatt atttagttta tatagtcact ttttgaaagt cgactattaa aagactattg
      241 cttgaagaat aacagctgtt attcttcctt ggacaaaagg tacaggagta tctaaaaagg
      301 agaattatca tgggacttat ttggacttta atcgtcggtg cgcttattgg tgtgattgct
      361 ggagcactta ctaaaaaagg tggttcaatg ggctggattg caaacatcgc tgctggtcta
      421 gttggttctt ccgttggtca agctttactt ggttcgtggg gcccttcttt agcaggaatg
      481 tctttgatcc cgtcagttat tggtgccgtt atcgttgtta tgatcacttc ttttgtacta
      541 aacaaaacta acaactagtt tcctctttat ttagtaagta cttacaaata gagaatgtta
      601 agtagaaaag aggtctgtat gtcaaaattg ttaaagatta gttattgtct tgtcggactt
      661 gttctattgt ctgtttttgg atgggtagtt ggaataaccg gaggctatat atatttacct
      721 tatagttatc gttggctaag ttggggtatg gatagttttc ccaatctctt agattcagct
      781 ttatcctatt attacttctg gacagccctt gtattatttg tt
//
LOCUS       DAUCWO010000140          773 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUCWO010000140 DAUCWO010000000
VERSION     DAUCWO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.140"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(39..749)
                     /gene="speh"
                     /locus_tag="VGY20_001746"
     CDS             complement(39..749)
                     /gene="speh"
                     /locus_tag="VGY20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeH"
                     /protein_id="HEQ4274557.1"
                     /translation="MRYNCRYSHIDKKIYSMIICLSFLLYSNVVQANSYNTTNRHNLE
                     SLYKHDSNLIEADSIKNSPDIVTSHMLKYSVKDKNLSVFFEKDWISQEFKDKEVDIYA
                     LSAQEVCECPGKRYEAFGGITLTNSEKKEIKVPVNVWDKSKQQPPMFITVNKPKVTAQ
                     EVDIKVRKLLIKKYDIYNNREQKYSKGTVTLDLNSGKDIVFDLYYFGNGDFNSMLKIY
                     SNNERIDSTQFHVDVSIS"
BASE COUNT          245 a          129 c           98 g          301 t
ORIGIN      
        1 tttatctatt tttaataaac agcttcttaa aaatataatt agctgattga cacatctaca
       61 tgaaattgag ttgagtctat tctctcgtta ttggaatata tttttagcat gctattaaag
      121 tctccattgc caaaataata caaatcaaaa acaatatctt tacctgaatt taaatctaag
      181 gtaacagttc ctttagagta tttttgttcc cggttattat agatatcgta tttcttaatc
      241 aataactttc taacttttat atccacttcc tgagcggtta ctttcggttt attgactgta
      301 ataaacatag gcggctgttg tttactttta tcccacacgt ttacaggaac tttaatttct
      361 tttttttctg aattagttaa tgtaattcca ccaaacgctt catacctttt ccctggacat
      421 tcacaaacct cttgtgcaga tagagcataa atatctactt ctttatcttt gaattcctgt
      481 gatatccaat ctttctcaaa aaaaactgac aaatttttat ccttgacact atatttcaac
      541 atatggcttg ttacaatatc tggagaattt tttatactat cggcttcaat caagttagaa
      601 tcatgcttat aaagcgattc tagattatgt ctattggttg tattataaga atttgcttga
      661 acaacattgg aatataaaag aaatgacaaa catataatca tgctgtagat tttcttatca
      721 atatgtgagt agcgacaatt atatctcatt ttttctcctt aagaagtgag agt
//
LOCUS       DAUCWO010000141          688 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUCWO010000141 DAUCWO010000000
VERSION     DAUCWO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.141"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            52..678
                     /locus_tag="VGY20_001747"
     CDS             52..678
                     /locus_tag="VGY20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4274558.1"
                     /translation="MEKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQA"
BASE COUNT          256 a           89 c          137 g          206 t
ORIGIN      
        1 gaaaattgtt aagaaacaag cgtcttgtaa accatttcaa agtatgatag aatggaaaag
       61 taccttaaaa agataggaaa aatgatgaca aagatgtatt atgatgaaaa tccagatagc
      121 ctacacgata ttcatgaagt aaaagtagag ctgcttaatc atccctttac ttttttgacg
      181 gattcaggtg tcttttcaaa gaaaatggtt gatttcggta gccaagtatt attagagaca
      241 ctaaatttca aggagaacga acgtgtactg gacttaggtt gcggctatgg tcctcttggg
      301 attagtctag ccaaggttca aagggtagac gcaaccttag tggacatcaa taatcgtgct
      361 cttgatttag ctagaaaaaa tgcaactaat aaccaagtgg cagtaactat ttttcaatca
      421 aatatttatg aaaatattag tggccatttt gagcatatta ttagtaatcc tcctattaga
      481 gcaggaaaga gagtagttca tagtattatt gaaaaaagca tagatttttt agtggttaat
      541 ggtgacttaa cgattgtgat tcaaaaaaaa caaggagctc ctagcgctaa agcaaaaatg
      601 gcaactattt ttggtaatgt tgagatatta aggaaagata aaggttatta tgtattaaga
      661 agtataaagc aagcctaatt taaataat
//
LOCUS       DAUCWO010000142          675 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUCWO010000142 DAUCWO010000000
VERSION     DAUCWO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.142"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            40..>675
                     /gene="spei"
                     /locus_tag="VGY20_001748"
     CDS             40..>675
                     /gene="spei"
                     /locus_tag="VGY20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047373492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeI"
                     /protein_id="HEQ4274559.1"
                     /translation="MSSVGVINLRNLYSTYDPTEVKGKINEGPPFSGSLFYKNIPYGN
                     SSIELKVELNSVEKANFFSGKRVDIFTLEYSPPCNSNIKKNSYGGITLSDGNRIDKKN
                     IPVNIFIDGVQQKYSYTDISTVSTDKKEVTIQELDVKSRYYLQKHFNIYGFGDVKDFG
                     RSSRFQSGFEEGNIIFHLNSGERISYNLFDTGHGDRESMLKKYSDNKTAYSD"
BASE COUNT          245 a           82 c          127 g          221 t
ORIGIN      
        1 tttatatcag ctataattcc aacgtgttac gcgtatgaga tgagtagtgt gggagttatt
       61 aatttaagga atttatactc tacatatgat ccaacagaag taaaaggaaa aataaatgaa
      121 ggtccgccat tttcaggtag tttgttctac aaaaatattc cttatggcaa tagttcgatt
      181 gaattaaaag tagaacttaa tagcgtagaa aaagctaatt ttttttctgg taaaagggtg
      241 gatatattta ctttggagta ttctcctccc tgtaactcta atataaaaaa gaattcatat
      301 ggaggtatta ctttaagcga cggtaataga attgataaaa aaaatatacc tgttaatatt
      361 ttcatagacg gcgttcaaca aaaatatagc tatacagata taagtacagt gagtactgat
      421 aagaaagagg ttacgattca ggaacttgat gtgaaatcaa gatattatct tcaaaaacat
      481 tttaatatat acggattcgg tgatgttaaa gattttggcc gctcctctag atttcaatct
      541 ggctttgagg aaggaaatat tatttttcat ttgaatagtg gagagagaat ttcttataat
      601 ctttttgata caggacatgg tgacagagag agtatgctaa aaaaatacag tgataataag
      661 accgcttatt ctgat
//
LOCUS       DAUCWO010000143          631 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUCWO010000143 DAUCWO010000000
VERSION     DAUCWO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.143"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(3..76)
                     /locus_tag="VGY20_001749"
     tRNA            complement(3..76)
                     /locus_tag="VGY20_001749"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:acg)
     gene            complement(93..177)
                     /locus_tag="VGY20_001750"
     tRNA            complement(93..177)
                     /locus_tag="VGY20_001750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Leu,seq:taa)
     gene            complement(186..257)
                     /locus_tag="VGY20_001751"
     tRNA            complement(186..257)
                     /locus_tag="VGY20_001751"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Gly,seq:gcc)
     gene            complement(270..342)
                     /locus_tag="VGY20_001752"
     tRNA            complement(270..342)
                     /locus_tag="VGY20_001752"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Thr,seq:tgt)
     gene            complement(353..434)
                     /locus_tag="VGY20_001753"
     tRNA            complement(353..434)
                     /locus_tag="VGY20_001753"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Leu,seq:tag)
     gene            complement(440..512)
                     /locus_tag="VGY20_001754"
     tRNA            complement(440..512)
                     /locus_tag="VGY20_001754"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Lys,seq:ttt)
     gene            complement(544..616)
                     /locus_tag="VGY20_001755"
     tRNA            complement(544..616)
                     /locus_tag="VGY20_001755"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Asp,seq:gtc)
BASE COUNT          165 a          183 c          136 g          147 t
ORIGIN      
        1 gatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
       61 gttgagctaa gggtgctatt tcaaaaaatt aatgccgagg accggaatcg aaccggtacg
      121 atgtaaacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggctcc
      181 tcaaaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt gatgttctac
      241 cactgaacta cgttcgcaac taatctcaat gccggctaca tgacttgaac acgcgaccct
      301 ctgattacaa atcagatgct ctaccaactg agctaagccg gctaatcttt tatgcgggtt
      361 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
      421 tccgccaaac ccgcttttat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
      481 gtcaattgct ctaccaactg agctaacgag tcttttcttc aaaaaatatg actactcttt
      541 gaacggtccc gacgggaatc gaacccgcga tcttcgccgt gacagggcga cgtgataacc
      601 gctacactac gggacctgta ttttccaata c
//
LOCUS       DAUCWO010000144          625 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUCWO010000144 DAUCWO010000000
VERSION     DAUCWO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.144"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            10..>625
                     /locus_tag="VGY20_001756"
     CDS             10..>625
                     /locus_tag="VGY20_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ4274560.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPNQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLE"
BASE COUNT          222 a          124 c          100 g          179 t
ORIGIN      
        1 aaataacata tgacaaaaaa acacttacta acacttcttc tcatctcttt ttttactagc
       61 tttttggtag cttgttcaac aacgaaagat aaagagcctc aaccgtctga ttcagaaatc
      121 attactcccc gactacacca agccgctcat caagataaac gcgctaactt tgaaaaaatt
      181 aaacttgcga ctgttgattc ctcatttaca ggaggaacaa gccttgaaga acttatttca
      241 ctctttggag agcctaacca acatgatcca aaaacagcag gcgaagtaac aatcgacgct
      301 tatacttggc agtttgatca agttactctc actgttaatc tttatcaaaa tagtagtatt
      361 gttaaaacca tctctaattt tacctttgca agagagttag gcttatcgca aaaagaatac
      421 caacaattac aaaaaggaat gtcttatgaa gacgttaaaa agatcttaac agaacctgat
      481 aattatagcc aagcgtcatc tagtgatcat caaactttgc aagcgatttg ggttagtggc
      541 ttaaagacag atacaagcgg agctaatatt tctctcgttt ttgaaaataa tcagttaaca
      601 gaaatgtctc aggtaggact tgaag
//
LOCUS       DAUCWO010000145          614 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUCWO010000145 DAUCWO010000000
VERSION     DAUCWO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.145"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..523)
                     /locus_tag="VGY20_001757"
     CDS             complement(<1..523)
                     /locus_tag="VGY20_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEQ4274561.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVC"
     gene            complement(516..>614)
                     /locus_tag="VGY20_001758"
     CDS             complement(516..>614)
                     /locus_tag="VGY20_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ4274562.1"
                     /translation="IFSLTNNISLQNDKVKRLTQEVALMKKEKTND"
BASE COUNT          215 a          100 c           96 g          203 t
ORIGIN      
        1 tacagacagc tactaatgct aaagtaagat ttttggaata taatattatt gctgtgtaca
       61 ccataaaaat aagagtattt ctgtaggcta gcgatttacc tgcaatatcc agccgttcat
      121 gttgttggaa cattccctga tataaatccg aaaaagcatc tgtcgatcta tagaaacaga
      181 ctagaaagac aattgtactc ttgtagctat ctgtcttagt taacgttaaa taaattactg
      241 ttatggctaa cattaataaa caagtcatca gtcttgccac taagtactga ctaaaggaat
      301 atttttcatt aatatctgtt gcttgataat tacgaacctg aaaaagtcca acaactacca
      361 tcatattagc aaaagaatag gcaaaggcgt aaatatctga atcagccgaa gttagtaacc
      421 tagtaaccac cataagtaat ataactgata ttacagctgt tgataaactt cctaacatat
      481 tccataaaaa aatcgtttgg ttttgttttg aaggattaat cattggtttt ctcttttttc
      541 ataagggcca cttcttgggt cagacgtttt actttatcat tttgaagtga aatattatta
      601 gtcagtgaaa aaat
//
LOCUS       DAUCWO010000146          534 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUCWO010000146 DAUCWO010000000
VERSION     DAUCWO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.146"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>534
                     /locus_tag="VGY20_001759"
     CDS             <1..>534
                     /locus_tag="VGY20_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ4274563.1"
                     /translation="TSIIEISVSNILKINKKATIVIKSTVPIGFTEYLRNRFHYNDII
                     FSPEFLREGSTIHDQLYPSRTIVGNESRNSQLFLDILTDISVEKDSPSLLVGSSEAEA
                     IKLFSNAYLAQKIAFFNELDTFAEMQNLDSKKIIEAIGYDQRIGNSHNNPSFGFGGYC
                     LPKDIKQLEYHFKEIPA"
BASE COUNT          186 a           81 c           90 g          177 t
ORIGIN      
        1 atacttccat tatcgaaatt tctgtaagta atattttaaa gataaacaaa aaggctacaa
       61 ttgtaataaa gtcgacagtt ccaattggtt ttacagaata tttaaggaat cgatttcact
      121 acaacgatat cattttttca cctgagttcc ttagggaagg atcaactatt catgatcaat
      181 tgtatccttc gagaactata gttggaaatg aatctagaaa ttctcaatta ttcttagaca
      241 tactaacaga tatatcggtt gaaaaagact cgccatcttt attagttggc tcttctgaag
      301 cagaagcgat aaagttattt tcgaatgcat acttggcaca aaaaattgct ttttttaatg
      361 agttggatac gtttgctgaa atgcaaaatt tggactcaaa aaaaattatt gaggctatag
      421 gatatgacca gagaatagga aattcgcaca ataatccttc tttcggtttt ggtgggtact
      481 gtcttcctaa ggatattaag caattagagt atcattttaa agaaattcca gcac
//
LOCUS       DAUCWO010000147          463 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAUCWO010000147 DAUCWO010000000
VERSION     DAUCWO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.147"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            21..>463
                     /locus_tag="VGY20_001760"
     CDS             21..>463
                     /locus_tag="VGY20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEQ4274564.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLD
                     AVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEE"
BASE COUNT          127 a           73 c          110 g          153 t
ORIGIN      
        1 ctatagatga ggtgactacg atgattgatt ttattatttc tattgatgat tgcgcagttg
       61 aattggatag tcgtcaatct tggaaaattc gctccccctt atcaaccatt ctatttcttg
      121 tcttcgtttg tcagttagct ggcattgaaa cctggaagga gatggaagat tttattgaaa
      181 tgaatgaacc attgtttgcg acctacgttg atttgagtga aggttgtccg tctcatgata
      241 ccttagagcg tgtgattagt cttgttaatt cagaccgttt aaaagagctt aaagttcaat
      301 ttgagcaatc attgacaagc ttagatgccg ttcatcaact gatttcagtg gacggtaaaa
      361 cgattcgagg caatcgaggt aaaaatcaga agcctgttca tattgtaacg gcttatgatg
      421 ggggtcatca tcttagtttg ggacaggtag cggttgagga gaa
//
LOCUS       DAUCWO010000148          425 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUCWO010000148 DAUCWO010000000
VERSION     DAUCWO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.148"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(62..135)
                     /locus_tag="VGY20_001761"
     tRNA            complement(62..135)
                     /locus_tag="VGY20_001761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Met,seq:cat)
     gene            complement(145..234)
                     /locus_tag="VGY20_001762"
     tRNA            complement(145..234)
                     /locus_tag="VGY20_001762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:tga)
     gene            complement(257..330)
                     /locus_tag="VGY20_001763"
     tRNA            complement(257..330)
                     /locus_tag="VGY20_001763"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Ile,seq:cat)
     gene            complement(351..424)
                     /locus_tag="VGY20_001764"
     tRNA            complement(351..424)
                     /locus_tag="VGY20_001764"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Met,seq:cat)
BASE COUNT          113 a          120 c           98 g           94 t
ORIGIN      
        1 ggagcttcaa tccattgctc taccaactga gctaccgagc caacaactac atacttatgt
       61 attgcgggag caggatttga acctacgacc ttcgggttat gagcccgacg agctacctaa
      121 ctgctccatc ccgcgatagt taaaaggagg atgtgggatt cgaacccacg cacgctttta
      181 cacgcctgac ggttttcaag accgttccct tcagccggac ttgggtaatc ctccaaaata
      241 atgattatct atgatatgga ccttgtagga ctcgaaccta cgaccgctcg gttatgagcc
      301 gagtgctcta accagttgag ctaaaggtcc caagtcatcc ataaaaataa tagcggcgaa
      361 ggggatcgaa cccccgacct cccgggtatg aaccggacgc tctagccagc tgagctacac
      421 cgcca
//
LOCUS       DAUCWO010000149          409 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUCWO010000149 DAUCWO010000000
VERSION     DAUCWO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.149"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>409
                     /locus_tag="VGY20_001765"
     CDS             <1..>409
                     /locus_tag="VGY20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEQ4274565.1"
                     /translation="LINLKERLQKYNITPISIASPLFKWNIEENHLESNLDLYGISAN
                     VTLNEQWLYVRKIIFIAKFLNIKYVRIFGGIGCSVDDFLNNELFLNLLKETEIIFLIE
                     NELGTSVSTADDLLKIGHFIDEKSIKNLKIWFD"
BASE COUNT          156 a           40 c           69 g          144 t
ORIGIN      
        1 agttaattaa tttaaaagaa cggttacaaa agtataatat cactccaatt tcaattgcaa
       61 gcccactttt taaatggaat atagaagaaa atcatttaga gagtaatctt gatttatacg
      121 gtatttcagc taatgttacg ttaaatgaac aatggcttta tgtgaggaaa attattttta
      181 ttgcaaaatt tttaaatatc aaatatgtaa gaatatttgg tgggattggg tgctcagtag
      241 atgacttctt gaataatgaa ctatttttga atttgttaaa agagactgaa attatctttt
      301 taattgagaa cgagttagga acttcagtga gcactgcaga cgatttgtta aaaataggac
      361 attttataga tgaaaaatct ataaaaaatc taaaaatctg gtttgatat
//
LOCUS       DAUCWO010000150          388 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUCWO010000150 DAUCWO010000000
VERSION     DAUCWO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.150"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          152 a           57 c           49 g          130 t
ORIGIN      
        1 cgcgtttttt tctttttcca tttaacaata aaaaaattca tgacgaacat ggtatcataa
       61 gagaattttt actttttaaa aaaaggtcaa agactttctg acaccaaata aaaatagaat
      121 tttttctaac taaaaataat agcacaatct ttggcagcaa tctgaattgt ttttccataa
      181 aagatttaag cacttacgtt tgcttaaaat ataggttatg tgacgtgcgt gcaagatcag
      241 attataacaa aatagttcca aataagccta tcactgagaa agcatcacag aagggttaaa
      301 ctttttttgt attagcgttt tatcagtaca acctaacact tatttttata aaaaaataaa
      361 aaagctattg acaatcttat acaagata
//
LOCUS       DAUCWO010000151          371 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUCWO010000151 DAUCWO010000000
VERSION     DAUCWO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.151"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>371
                     /locus_tag="VGY20_001766"
     CDS             <1..>371
                     /locus_tag="VGY20_001766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ4274566.1"
                     /translation="SLLCRYGLTSAAALLLTFGGVSAVKADEQSNIEALQSQLQALRE
                     NLPKVGDNIPKHDVFRQHVFYKHLEEFEARLDFYLKTLDGSLSQLQGPRGEKGDQGPR
                     GEQGLRGEIGPEGPQGKTGPAG"
BASE COUNT          104 a           76 c          100 g           91 t
ORIGIN      
        1 agccttcttt gtcgctatgg cttaacctca gcggctgctc ttttgctgac ctttggaggt
       61 gtaagcgcgg ttaaggcgga tgagcaaagt aatattgaag cactacagag tcaattacag
      121 gctttacgcg aaaatttgcc taaggtcgga gataatattc ctaagcatga tgttttcagg
      181 caacatgtgt tttataaaca tttggaagag tttgaagctc gactggattt ttacttaaaa
      241 acgttggatg ggagtctatc ccaactgcaa ggtccacgtg gtgaaaaagg agaccagggc
      301 ccacgtggtg aacaagggct acgaggcgaa atcggtcctg aaggcccaca aggcaaaacc
      361 ggcccagcag g
//
LOCUS       DAUCWO010000152          268 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUCWO010000152 DAUCWO010000000
VERSION     DAUCWO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.152"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>268
                     /locus_tag="VGY20_001767"
     CDS             <1..>268
                     /locus_tag="VGY20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ4274567.1"
                     /translation="ELVYRPAKQSEKAEAGDYEHLCQIWEGLTVGTAKVWATEMRDHP
                     DFKQFVLNPTHKIVFIDYEGFRLFVKWKSRNRYKPKKETLVEMLE"
BASE COUNT           93 a           47 c           59 g           69 t
ORIGIN      
        1 gaattagttt acagaccagc taagcaatct gaaaaagcag aagctggtga ctatgaacac
       61 ctttgccaaa tctgggaagg tttgacagtc ggaacggcta aggtttgggc gacagaaatg
      121 cgtgatcatc cagactttaa acagtttgta ttaaatccaa cgcataaaat cgtattcatt
      181 gattacgaag gtttccgttt atttgttaaa tggaaaagtc gtaaccgcta taaacccaag
      241 aaggaaaccc tggtagaaat gcttgaaa
//
LOCUS       DAUCWO010000153          254 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUCWO010000153 DAUCWO010000000
VERSION     DAUCWO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.153"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(50..>254)
                     /locus_tag="VGY20_001768"
     CDS             complement(50..>254)
                     /locus_tag="VGY20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ4274568.1"
                     /translation="FNPLHTKLHEYFGKRGAKIIDGLWVLIFQGIEAFSLWSDKINGG
                     KYFFSKDDVVLIHTFLEGKIKGK"
BASE COUNT           90 a           47 c           24 g           93 t
ORIGIN      
        1 tcatctagtt tgtctgatat ttcatcaaga tttactgtaa tatgaaaagt cattttcctt
       61 ttatcttccc ttctagaaat gtatgaatta ggacaacatc atcttttgaa aaaaaatatt
      121 taccaccatt aattttatca ctccacaatg aaaatgcttc aattccctga aaaatcaaaa
      181 cccataaacc atcaattatt tttgctcctc ttttaccaaa atattcatga agtttcgtat
      241 gtaaaggatt gaaa
//
LOCUS       DAUCWO010000154          237 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134036
            SAMN37094598-rid19214033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUCWO010000154 DAUCWO010000000
VERSION     DAUCWO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094598
            Sequence Read Archive: SRR25711382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859356.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,729
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 37 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 10 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094598-rid19214033.denovo.154"
                     /strain="TG134036"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-12"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT           81 a           37 c           29 g           90 t
ORIGIN      
        1 ccgacaaaaa atacaaagat tctaaaaaag tcaagctatt ttagtttagc ctttgtcaat
       61 cgtatcagtt caaatagaaa atacaagttt tgacaagaat ttatttatgt aacaaacttt
      121 gtttttgatt cttcagacta tctagcgata atgttatctg atccttgtat tcaaccaatt
      181 gatgttaaag aagctgtctt catgattctc taatattcct tcaattttag ctttata
//
